title: Hypercholesterolaemia, signs of islet microangiopathy and altered angiogenesis precede onset of type 2 diabetes in the Goto-Kakizaki (GK) rat.
abstract: OBJECTIVE
  The adult non-obese Goto-Kakizaki (GK) rat model of type 2 diabetes, particularly females, carries in addition to hyperglycaemia a genetic predisposition towards dyslipidaemia, including hypercholesterolaemia. As cholesterol-induced atherosclerosis may be programmed in utero, we looked for signs of perinatal lipid alterations and islet microangiopathy. We hypothesise that such alterations contribute towards defective pancreas/islet vascularisation that might, in turn, lead to decreased beta cell mass. Accordingly, we also evaluated islet inflammation and endothelial activation in both prediabetic and diabetic animals.
  
  
  METHODS
  Blood, liver and pancreas were collected from embryonic day (E)21 fetuses, 7-day-old prediabetic neonates and 2.5-month-old diabetic GK rats and Wistar controls for analysis/quantification of: (1) systemic variables, particularly lipids; (2) cholesterol-linked hepatic enzyme mRNA expression and/or activity; (3) pancreas (fetuses) or collagenase-isolated islet (neonates/adults) gene expression using Oligo GEArray microarrays targeted at rat endothelium, cardiovascular disease biomarkers and angiogenesis, and/or RT-PCR; and (4) pancreas endothelial immunochemistry: nestin (fetuses) or von Willebrand factor (neonates).
  
  
  RESULTS
  Systemic and hepatic cholesterol anomalies already exist in GK fetuses and neonates. Hyperglycaemic GK fetuses exhibit a similar percentage decrease in total pancreas and islet vascularisation and beta cell mass. Normoglycaemic GK neonates show systemic inflammation, signs of islet pre-microangiopathy, disturbed angiogenesis, collapsed vascularisation and altered pancreas development. Concomitantly, GK neonates exhibit elevated defence mechanisms.
  
  
  CONCLUSIONS
  These data suggest an autoinflammatory disease, triggered by in utero programming of cholesterol-induced islet microangiopathy interacting with chronic hyperglycaemia in GK rats. During the perinatal period, GK rats show also a marked deficient islet vascularisation in conjunction with decreased beta cell mass.
authors: Laboratoire B2PE, Biologie et Pathologie du Pancréas Endocrine, Unité Biologie Fonctionnelle et Adaptative-EAC CNRS 4413, Université Paris-Diderot, Bâtiment Lamarck, Case 7104, 5 rue Marie-Andrée Lagroua Weill-Hallé, 75205, Paris Cedex 13, France. marie-helene.giroix@univ-paris-diderot.fr
authors: M-H
authors: MH
authors: Giroix
authors: J-C
authors: JC
authors: Irminger
authors: G
authors: G
authors: Lacraz
authors: C
authors: C
authors: Noll
authors: S
authors: S
authors: Calderari
authors: J A
authors: JA
authors: Ehses
authors: J
authors: J
authors: Coulaud
authors: M
authors: M
authors: Cornut
authors: N
authors: N
authors: Kassis
authors: F
authors: F
authors: Schmidlin
authors: J-L
authors: JL
authors: Paul
authors: M
authors: M
authors: Kergoat
authors: N
authors: N
authors: Janel
authors: P A
authors: PA
authors: Halban
authors: F
authors: F
authors: Homo-Delarche
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D003925:Diabetic Angiopathies / Q000503:physiopathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006937:Hypercholesterolemia / Q000503:physiopathology*; D007328:Insulin / Q000097:blood; D050417:Insulin-Secreting Cells / Q000473:pathology; D007515:Islets of Langerhans / Q000098:blood supply; D008297:Male; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D011237:Predictive Value of Tests; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007328:Insulin