title: Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes.
abstract: Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolytic erbB variants, was evaluated using RT-PCR. Epidermal growth factor receptor (EGFR)/erbB1 expression was confirmed. Wild-type (wt) ErbB2, human erbB2 (HER2)-extracellular domain (ECD) and herstatin mRNA expression were analyzed. Restriction analysis confirmed wt expression. 3T3-L1 cells exhibited HER2-ECD and herstatin mRNA expression, although at extremely low levels (compared to the control cell lines). ErbB3 cDNA was amplified using mouse mammary tumors and rat acines as positive controls. ErbB4 was not positively identified in these cells. In conclusion, this study demonstrates that 3T3-L1 cells express EGFR/erbB1, erbB2 and erbB3, and possibly, certain erbB2 splice or proteolytic variants, which are worth pursuing.
authors: Facultad de Ciencias Médicas, Pontificia Universidad Católica Argentina, Alicia Moreau de Justo 1600, 3º Piso (C1107AFF), Buenos Aires, Argentina. eleonora_pagano@yahoo.com.ar
authors: Eleonora
authors: E
authors: Pagano
authors: Vanina
authors: V
authors: Fontana
authors: Juan Carlos
authors: JC
authors: Calvo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D017398:Alternative Splicing / Q000235:genetics*; D000818:Animals; D049109:Cell Proliferation; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D020893:Receptor, ErbB-3 / Q000235:genetics / Q000378:metabolism; D066247:Receptor, ErbB-4; D017978:Receptors, Growth Factor / Q000235:genetics* / Q000378:metabolism*
chemical_list: D066246:ErbB Receptors; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D066247:Receptor, ErbB-4; D017978:Receptors, Growth Factor; C434665:herstatin; C587689:ERBB4 protein, human; C587690:Erbb4 protein, mouse; C587691:Erbb4 protein, rat