title: Real-time detection of blaKPC in clinical samples and surveillance specimens.
abstract: A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity.
authors: Pathology & Laboratory Medicine, NorthShore University Health System, 2650 Ridge Avenue, Evanston, IL 60201, USA. kmangold@northshore.org
authors: Kathy A
authors: KA
authors: Mangold
authors: Kristine
authors: K
authors: Santiano
authors: Ronit
authors: R
authors: Broekman
authors: Catherine A
authors: CA
authors: Krafft
authors: Barbara
authors: B
authors: Voss
authors: Vivien
authors: V
authors: Wang
authors: Donna M
authors: DM
authors: Hacek
authors: Elena A
authors: EA
authors: Usacheva
authors: Richard B
authors: RB
authors: Thomson
authors: Karen L
authors: KL
authors: Kaul
authors: Lance R
authors: LR
authors: Peterson
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D017931:DNA Primers; D001618:beta-Lactamases