title: Highly parallel fabrication of nanopatterned surfaces with nanoscale orthogonal biofunctionalization imprint lithography.
abstract: Large areas of nanopatterns of specific chemical functionality are needed for biological experiments and biotechnological applications. We present nanoscale orthogonal biofunctionalization imprint lithography (NOBIL), a parallel top-down imprinting and lift-off technique based on step-and-flash imprint lithography (SFIL) that is able to create centimetre-scale areas of nanopatterns of two biochemical functionalities. A photoresist precursor is polymerized with a template in place, and the thin resist layer is etched to create an undercut for lift-off. Gold nano-areas on a silicon dioxide background are then independently functionalized using self-assembly that translates the nanopattern into a cell-adhesive/cell-rejective functionality pattern. We demonstrate the technique by creating fibronectin areas down to a pattern size of 60 nm against a polyethylene glycol (PEG) background, and show initial results of cells stably seeded over an array of 1 mm(2) areas of controlled size and pitch.
authors: Department of Biomedical Engineering and Center for Nano and Molecular Science and Technology, The University of Texas at Austin, 1 University Station, C0800, Austin, TX 78712, USA.
authors: Harold E
authors: HE
authors: Gaubert
authors: Wolfgang
authors: W
authors: Frey