title: Sequence analysis of the spliced-leader intergenic region (SL-IR) and random amplified polymorphic DNA (RAPD) of Trypanosoma rangeli strains isolated from Rhodnius ecuadoriensis, R. colombiensis, R. pallescens and R. prolixus suggests a degree of co-evolution between parasites and vectors.
abstract: Spliced leader intergenic region (SL-IR) sequences from 23 Trypanosoma rangeli strains isolated from the salivary glands of Rhodnius colombiensis, R. ecuadoriensis, R. pallescens and R. prolixus and two human strains revealed the existence of 4 genotypes with CA, GT, TA, ATT and GTAT microsatellite repeats and the presence of insertions/deletions (INDEL) and single nucleotide polymorphism (SNP) characterizing each genotype. The strains isolated from the same vector species or the same Rhodnius evolutionary line presented the same genotypes, even in cases where strains had been isolated from vectors captured in geographically distant regions. The dendrogram constructed from the SL-IR sequences separated all of them into two main groups, one with the genotypes isolated from R. prolixus and the other group containing three well defined sub-groups with the genotypes isolated from R. pallescens, R. colombiensis and R. ecuadoriensis. Random amplified polymorphic DNA (RAPD) analysis showed the same two main groups and sub-groups supporting strict T. rangeli genotypes' association with Rhodnius species. Combined with other studies, these results suggest a possible co-evolutionary association between T. rangeli genotypes and their vectors.
authors: Laboratorio de Investigaciones en Parasitología Tropical-LIPT, Universidad del Tolima, AA 546, Altos de Santa Helena, Ibagué, Colombia.
authors: Daniel Alfonso
authors: DA
authors: Urrea
authors: Felipe
authors: F
authors: Guhl
authors: Claudia Patricia
authors: CP
authors: Herrera
authors: Alejandra
authors: A
authors: Falla
authors: Julio César
authors: JC
authors: Carranza
authors: César
authors: C
authors: Cuba-Cuba
authors: Omar
authors: O
authors: Triana-Chávez
authors: Edmundo C
authors: EC
authors: Grisard
authors: Gustavo Adolfo
authors: GA
authors: Vallejo
mesh_terms: D000818:Animals; D005075:Biological Evolution; D021901:DNA, Intergenic / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D019143:Evolution, Molecular; D014644:Genetic Variation; D018503:Genome, Protozoan / Q000235:genetics*; D005838:Genotype; D006790:Host-Parasite Interactions; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology; D010802:Phylogeny; D020038:RNA, Spliced Leader / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012239:Rhodnius / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D057509:Trypanosoma rangeli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D021901:DNA, Intergenic; D016054:DNA, Protozoan; D020038:RNA, Spliced Leader