title: Simultaneous determination of cyadox and its metabolites in plasma by high-performance liquid chromatography tandem mass spectrometry.
abstract: Cyadox is a novel antimicrobial growth-promoter of the quinoxalines. For food safety and pharmacokinetic studies, a convenient, sensitive and reproducible LC-ESI-MS/MS method was developed for the simultaneous determination of cyadox and its major metabolites, quinoxaline-2-carboxylic acid, 1,4-bisdesoxycyadox, cyadox-1-monoxide and cyadox-4-monoxide in chicken plasma. Plasma sample was subjected to a simple deproteinisation with acetonitrile. Analysis was performed on a C18 column by detection with mass spectrometry in multiple reaction monitoring mode. A gradient elution program with 0.2% formic acid, methanol and acetonitrile was performed at a flow rate of 0.2 mL/min. The decision limits (CCαs) of five analytes in plasma ranged from 1.0 to 4.0 μg/L, and the detection capabilities (CCβs) were <10 μg/L. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The extraction recoveries of five analytes were between 87.4 and 93.9% in plasma at the spiked levels of 5 (10)-200 μg/L with the relative standard deviations <10% for each analyte. The developed method demonstrated a satisfactory applicability in real plasma samples.
authors: Department of Pharmacology and Toxicology, College of Veterinary Medicine, South China Agriculture University, Guangzhou, P. R. China.
authors: Limin
authors: L
authors: He
authors: Kaiyong
authors: K
authors: Liu
authors: Yijuan
authors: Y
authors: Su
authors: Jiahui
authors: J
authors: Zhang
authors: Yahong
authors: Y
authors: Liu
authors: Zhenling
authors: Z
authors: Zeng
authors: Binghu
authors: B
authors: Fang
authors: Guijun
authors: G
authors: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D011810:Quinoxalines / Q000097:blood / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D011810:Quinoxalines; C020728:cyadox