title: Phenolic antioxidants tert-butyl-bisphenol and vitamin E decrease oxidative stress and enhance vascular function in an animal model of rhabdomyolysis yet do not improve acute renal dysfunction.
abstract: Rhabdomyolysis (RM) caused by severe burn releases extracellular myoglobin (Mb) that accumulates in the kidney. Extracellular Mb is a pro-oxidant. This study tested whether supplementation with tert-butyl-bisphenol (BP) or vitamin E (Vit E, as α-tocopherol) at 0.12% w/w in the diet inhibits acute renal failure (ARF) in an animal model of RM. After RM-induction in rats, creatinine clearance decreased (p < 0.01), proteinuria increased (p < 0.001) and renal-tubule damage was detected. Accompanying ARF, biomarkers of oxidative stress (lipid oxidation and hemeoxygenase-1 (HO-1) gene and protein activity) increased in the kidney (p < 0.05). Supplemented BP or Vit E decreased lipid oxidation (p < 0.05) and HO-1 gene/activity and restored aortic cyclic guanylyl monophosphate in control animals (p < 0.001), yet ARF was unaffected. Antioxidant supplementation inhibited oxidative stress, yet was unable to ameliorate ARF in this animal model indicating that oxidative stress in kidney and vascular cells may not be causally related to renal dysfunction elicited by RM.
authors: Discipline of Pathology, Redox Biology Group, The University of Sydney, Sydney, NSW, Australia.
authors: Hyun Bo
authors: HB
authors: Kim
authors: Anu
authors: A
authors: Shanu
authors: Sarah
authors: S
authors: Wood
authors: Sarah N
authors: SN
authors: Parry
authors: Michael
authors: M
authors: Collet
authors: Aisling
authors: A
authors: McMahon
authors: Paul K
authors: PK
authors: Witting
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology / Q000235:genetics; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D051547:Heme Oxygenase-1 / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D009211:Myoglobin / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000097:blood / Q000378:metabolism; D012206:Rhabdomyolysis / Q000150:complications / Q000188:drug therapy*; D024502:alpha-Tocopherol / Q000627:therapeutic use*
chemical_list: C568353:3,3',5,5'-tetra-tert-butyl-biphenyl-4,4'-diol; D000975:Antioxidants; D009211:Myoglobin; D010636:Phenols; D017382:Reactive Oxygen Species; D003404:Creatinine; D051547:Heme Oxygenase-1; D024502:alpha-Tocopherol