title: A novel amino-oligosaccharide isolated from the culture of Streptomyces strain PW638 is a potent inhibitor of α-amylase.
abstract: A novel amino-oligosaccharide, named SF638-1, was isolated from the culture filtrate of the Streptomyces strain PW638. Its chemical structure was determined by electrospray ionization tandem mass spectrometry (ESI-MS/MS) and two-dimensional nuclear magnetic resonance spectroscopy. The novel compound was a mixed inhibitor of human pancreatic α-amylase, with a K(i) value in the same order of magnitude as that of the α-amylase inhibitor, acarbose. SF638-1 inhibited starch hydrolysis and glucose transfer in vitro, and suppressed postprandial blood glucose elevation in vivo. These results suggest that SF638-1 may be a potent antidiabetic agent.
authors: Nankai University, Tianjin, People's Republic of China.
authors: Peng
authors: P
authors: Meng
authors: Yuanqiang
authors: Y
authors: Guo
authors: Qi
authors: Q
authors: Zhang
authors: Jie
authors: J
authors: Hou
authors: Fang
authors: F
authors: Bai
authors: Peng
authors: P
authors: Geng
authors: Gang
authors: G
authors: Bai
mesh_terms: D000606:Amino Sugars / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D002240:Carbohydrate Sequence; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013302:Streptomyces / Q000737:chemistry*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D000606:Amino Sugars; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D000516:alpha-Amylases