title: Polymersome-loaded capsules for controlled release of DNA.
abstract: The formation of a novel drug-delivery carrier for the controlled release of plasmid DNA that comprises layer-by-layer polymer capsules subcompartmentalized with pH-sensitive nanometer-sized polymersomes is reported. The amphiphilic diblock copolymer poly(oligoethylene glycol methacrylate)-block-poly(2-(diisopropylamino)ethyl methacrylate) forms polymersomes at physiological pH, but transitions to unimeric polymer chains upon acidification to cellular endocytic pH. These polymersomes can thus release an encapsulated payload in response to a change in pH from physiological to endocytic conditions. Multicomponent layer-by-layer capsules are formed by exploiting the ability of tannic acid to act as an efficient hydrogen-bond donor for both the polymersomes and poly(N-vinyl pyrrolidone) at physiological pH. These capsules show release of a plasmid DNA payload encapsulated within the polymersome subcompartments in response to changes in pH between physiological and endocytic conditions.
authors: Department of Chemical and Biomolecular Engineering, The University of Melbourne, Victoria 3010, Australia.
authors: Hannah
authors: H
authors: Lomas
authors: Angus P R
authors: AP
authors: Johnston
authors: Georgina K
authors: GK
authors: Such
authors: Zhiyuan
authors: Z
authors: Zhu
authors: Kang
authors: K
authors: Liang
authors: Martin P
authors: MP
authors: van Koeverden
authors: Siripong
authors: S
authors: Alongkornchotikul
authors: Frank
authors: F
authors: Caruso
mesh_terms: D002214:Capsules; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D002214:Capsules; D002232:Carbocyanines; D011108:Polymers; C085321:cyanine dye 5; D004247:DNA