title: Influence of lipid membrane rigidity on properties of supporting polymer.
abstract: Temperature-sensitive hydrogel polymers are utilized as responsive layers in various applications. Although the polymer's native characteristics have been studied extensively, details concerning its properties during interaction with biorelated structures are lacking. This work investigates the interaction between a thermoresponsive polymer cushion and different lipid membrane capping layers probed by neutron reflectometry. N-isopropylacrylamide copolymerized with methacroylbenzophenone first supported a lipid bilayer composed of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and subsequently 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The polymer-membrane systems were investigated above and below the polymer transition temperature (37 and 25°C). Although the same cushion supported each lipid membrane, the polymer hydration profile and thickness were markedly different for DPPE and DPPC systems. Because DPPE and DPPC have different bending rigidities, these results establish that the polymer-membrane interaction is critically mediated by the mechanics of the membrane, providing better insight into cell-hydrogel interactions.
authors: Lujan Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
authors: Michael S
authors: MS
authors: Jablin
authors: Manish
authors: M
authors: Dubey
authors: Mikhail
authors: M
authors: Zhernenkov
authors: Ryan
authors: R
authors: Toomey
authors: Jarosław
authors: J
authors: Majewski
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D017666:Deuterium Oxide / Q000737:chemistry; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000737:chemistry*; D009502:Neutrons; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D011108:Polymers; D014867:Water; C052970:poly-N-isopropylacrylamide; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; D017666:Deuterium Oxide