title: Tight protein-DNA interactions favor gene silencing.
abstract: The heterochromatin-like structure formed by the yeast silent information regulator complex (SIR) represses transcription at the silent mating type loci and telomeres. Here, we report that tight protein-DNA complexes induce ectopic recruitment of the SIR complex, promoting gene silencing and changes in subnuclear localization when cis-acting elements are nearby. Importantly, lack of the replication fork-associated helicase Rrm3 enhances this induced gene repression. Additionally, Sir3 and Sir4 are enriched genome-wide at natural replication pause sites, including tRNA genes. Consistently, inserting a tRNA gene promotes SIR-mediated silencing of a nearby gene. These results reveal that replication stress arising from tight DNA-protein interactions favors heterochromatin formation.
authors: Institut Curie, Paris, F-75248 France.
authors: Marion
authors: M
authors: Dubarry
authors: Isabelle
authors: I
authors: Loïodice
authors: Chunlong L
authors: CL
authors: Chen
authors: Claude
authors: C
authors: Thermes
authors: Angela
authors: A
authors: Taddei
mesh_terms: D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D017930:Genes, Reporter / Q000235:genetics; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: C084122:SIR3 protein, S cerevisiae; C061573:SIR4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; C459081:Rrm3 protein, S cerevisiae; D004265:DNA Helicases