title: Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air.
abstract: Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×10(4) to 6.92×10(4) copies/m(3) air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.
authors: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, Shandong, China.
authors: Jing
authors: J
authors: Lv
authors: Baozhi
authors: B
authors: Wei
authors: Tongjie
authors: T
authors: Chai
authors: Xianzhu
authors: X
authors: Xia
authors: Zengmin
authors: Z
authors: Miao
authors: Meiling
authors: M
authors: Yao
authors: Yuwei
authors: Y
authors: Gao
authors: Rong
authors: R
authors: Huang
authors: Huili
authors: H
authors: Yang
authors: Uwe
authors: U
authors: Roesler
mesh_terms: D000388:Air / Q000032:analysis; D000391:Air Microbiology; D000818:Animals; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000821:virology*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers