title: Megapixel digital PCR.
abstract: We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.
authors: Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Kevin A
authors: KA
authors: Heyries
authors: Carolina
authors: C
authors: Tropini
authors: Michael
authors: M
authors: Vaninsberghe
authors: Callum
authors: C
authors: Doolin
authors: Oleh I
authors: OI
authors: Petriv
authors: Anupam
authors: A
authors: Singhal
authors: Kaston
authors: K
authors: Leung
authors: Curtis B
authors: CB
authors: Hughesman
authors: Carl L
authors: CL
authors: Hansen
mesh_terms: D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004867:Equipment Design; D020869:Gene Expression Profiling / Q000295:instrumentation*; D044085:Microfluidics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*