title: Secretion of N-terminal domain of α-dystroglycan in cerebrospinal fluid.
abstract: α-Dystroglycan (α-DG) plays crucial roles in maintaining the stability of cells. We demonstrated previously that the N-terminal domain of α-DG (α-DG-N) is secreted by cultured cells into the culture medium. In the present study, to clarify its function in vivo, we generated a monoclonal antibody against α-DG-N and investigated the secretion of α-DG-N in human cerebrospinal fluid (CSF). Interestingly, we found that a considerable amount of α-DG-N was present in CSF. α-DG-N in CSF was a sialylated glycoprotein with both N- and O-linked glycan. These observations suggest that secreted α-DG-N may be transported via CSF and have yet unidentified effects on the nervous system.
authors: Department of Neurology, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. f-saito@med.teikyo-u.ac.jp
authors: Fumiaki
authors: F
authors: Saito
authors: Yuko
authors: Y
authors: Saito-Arai
authors: Ayami
authors: A
authors: Nakamura-Okuma
authors: Miki
authors: M
authors: Ikeda
authors: Hiroki
authors: H
authors: Hagiwara
authors: Toshihiro
authors: T
authors: Masaki
authors: Teruo
authors: T
authors: Shimizu
authors: Kiichiro
authors: K
authors: Matsumura
mesh_terms: D002478:Cells, Cultured; D049030:Dystroglycans / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D009474:Neurons / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: C483081:DAG1 protein, human; D006023:Glycoproteins; D049030:Dystroglycans; D019158:N-Acetylneuraminic Acid