title: Loss of surface antigens is a conserved feature of apoptotic lymphocytes from several mammalian species.
abstract: Human lymphocytes lose the expression of lineage antigens (LAgs) along apoptosis. Our aim was to extent our previous studies of LAg loss to rodent species, quantifying LAg expression on apoptotic murine lymphocytes using flow cytometry to measure alterations in cell permeability, phosphatidylserine exposure and caspase activation of CD3, CD5, CD4, CD8, CD19 and CD28 LAgs in highly purified lymphocyte populations. We found loss of expression by apoptotic cells of all LAgs studied in the three species analyzed except for CD3 antigen in mouse. We also found an early, rapid and dramatic reduction in the expression of CD28 by early apoptotic cells. We found several homologies across the three species in the kinetic of loss of several LAgs such as CD5, CD4 and CD28. These data suggest that the loss of expression of LAgs by apoptotic lymphocytes is a common and conserved feature of lymphocytes undergoing apoptosis in several mammalian species.
authors: Department of Medicine, University of Alcalá, Madrid, Spain. david.diaz@uah.es
authors: David
authors: D
authors: Diaz
authors: Luis
authors: L
authors: Chara
authors: Julio
authors: J
authors: Chevarria
authors: Maria
authors: M
authors: Ubeda
authors: Leticia
authors: L
authors: Muñoz
authors: Hugo
authors: H
authors: Barcenilla
authors: Miguel Angel
authors: MA
authors: Sánchez
authors: Zaida
authors: Z
authors: Moreno
authors: Jorge
authors: J
authors: Monserrat
authors: Agustin
authors: A
authors: Albillos
authors: Alfredo
authors: A
authors: Prieto
authors: Melchor
authors: M
authors: Alvarez-Mon
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology / Q000378:metabolism; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D015704:CD4 Antigens / Q000276:immunology / Q000378:metabolism; D018956:CD5 Antigens / Q000276:immunology / Q000378:metabolism; D016827:CD8 Antigens / Q000276:immunology / Q000378:metabolism; D053148:Caspase 3 / Q000276:immunology / Q000378:metabolism; D053181:Caspase 8 / Q000276:immunology / Q000378:metabolism; D053453:Caspase 9 / Q000276:immunology / Q000378:metabolism; D020169:Caspases / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D005434:Flow Cytometry; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018941:Antigens, CD19; D000954:Antigens, Surface; D018106:CD28 Antigens; D017252:CD3 Complex; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases