title: Visual detection of melamine in milk samples based on label-free and labeled gold nanoparticles.
abstract: Melamine that can cause serious damage to the organs of animal or human beings was found to bind to polythymine via hydrogen bonding. With this novel discovery, colorimetric detection of melamine based on label-free and labeled gold nanoparticles was developed, respectively. Both of the methods revealed good selectivity for melamine over other components that may exist in milk and good anti-influence ability. The raw milk samples were pretreated according to the National standard method combined with a solid phase extraction monolithic column. The accurate quantification of melamine as low as 41.7 nM and 46.5 nM was obtained, respectively. It also guarantees fast and reliable readout with naked eyes, making visual detection possible. Further comparison between label-free and labeled based methods was discussed in this paper.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors: Hui
authors: H
authors: Huang
authors: Li
authors: L
authors: Li
authors: Guohua
authors: G
authors: Zhou
authors: Zhihong
authors: Z
authors: Liu
authors: Qiao
authors: Q
authors: Ma
authors: Yuqi
authors: Y
authors: Feng
authors: Guoping
authors: G
authors: Zeng
authors: Philip
authors: P
authors: Tinnefeld
authors: Zhike
authors: Z
authors: He
mesh_terms: D000818:Animals; D003124:Colorimetry / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D014227:Triazines / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D014227:Triazines; D006046:Gold; C011907:melamine