title: Production of transgenic bovine cloned embryos using piggybac transposition.
abstract: Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.
authors: Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute, Seoul National University, Seoul 151–742, Republic of Korea.
authors: Su
authors: S
authors: Kim
authors: Islam M
authors: IM
authors: Saadeldin
authors: Woo Jae
authors: WJ
authors: Choi
authors: Song Jeon
authors: SJ
authors: Lee
authors: Won Wu
authors: WW
authors: Lee
authors: Bong Han
authors: BH
authors: Kim
authors: Hyo Jun
authors: HJ
authors: Han
authors: Du Hee
authors: du H
authors: Bang
authors: Byeong Chun
authors: BC
authors: Lee
authors: Goo
authors: G
authors: Jang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002417:Cattle / Q000196:embryology / Q000235:genetics*; D004318:Doxycycline / Q000378:metabolism; D005347:Fibroblasts; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering / Q000379:methods / Q000662:veterinary*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014162:Transfection / Q000379:methods / Q000662:veterinary; D000097573:Red Fluorescent Protein
chemical_list: D004318:Doxycycline; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein