title: Sandaracinus amylolyticus gen. nov., sp. nov., a starch-degrading soil myxobacterium, and description of Sandaracinaceae fam. nov.
abstract: A novel starch-degrading myxobacterium designated NOSO-4(T) (new organism of the Sorangiineae strain 4) was isolated in 1995 from a soil sample containing plant residues, collected in Lucknow, Uttar Pradesh, India. The novel bacterium shows typical myxobacterial characteristics such as gram-negative, rod-shaped vegetative cells, swarming colonies, fruiting body-like aggregates and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic. Based on 16S rRNA gene sequences, NOSO-4(T) shows highest similarity (96.2 %) with the unidentified bacterial strain O29 (accession no. FN554397), isolated from leek (Allium porrum) rhizosphere, and to the myxobacteria Jahnella thaxteri (88.9 %) and Chondromyces pediculatus (88.5 %). Major fatty acids are C(17 : 1) 2-OH, C(20 : 4)ω6 (arachidonic acid), and the straight-chain fatty acids C(17 : 0), C(15 : 0) and C(16 : 0). The genomic DNA G+C content of the novel isolate is 66.8 mol%. It is proposed that strain NOSO-4(T) represents a novel species in a new genus, i.e. Sandaracinus amylolyticus gen. nov., sp. nov., but also belongs to a new family, Sandaracinaceae fam. nov. The type strain of the type species, S. amylolyticus sp. nov., is NOSO-4(T) ( = DSM 53668(T) = NCCB 100362(T)).
authors: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors: Kathrin I
authors: KI
authors: Mohr
authors: Department of Pharmaceutical Biotechnology, Saarland University, Campus C2 3 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus C2 3 66123 Saarbrücken, Germany.
authors: Ronald O
authors: RO
authors: Garcia
authors: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors: Klaus
authors: K
authors: Gerth
authors: Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors: Herbert
authors: H
authors: Irschik
authors: Department of Pharmaceutical Biotechnology, Saarland University, Campus C2 3 66123 Saarbrücken, Germany.|Department of Microbial Natural Products, Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) - Helmholtz Centre for Infection Research (HZI), Saarland University, Campus C2 3 66123 Saarbrücken, Germany.|Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
authors: Rolf
authors: R
authors: Müller
mesh_terms: D000332:Aerobiosis; D001433:Bacteriolysis; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D052836:Heterotrophic Processes; D007194:India; D008124:Locomotion; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D013213:Starch