title: Isolation and characterization of microsatellite loci from apomictic Hypericum perforatum (Hypericaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to study the origins and evolution of apomictic Hypericum perforatum, a polyploid invasive perennial herb indigenous to Europe that produces compounds of medicinal/pharmaceutical importance.
  
  
  RESULTS
  Eleven primer sets were developed using a non-radioactive protocol. All loci were polymorphic, showing from 2 to 9 alleles per locus. Observed and expected heterozygosity averaged 0.861 and 0.772, respectively, across four studied populations. Most primers also amplified successfully in eight other Hypericum species.
  
  
  CONCLUSIONS
  Markers developed in this study provide genetic tools for studies of apomixis and invasiveness, both on the intra- and interspecific levels.
authors: Apomixis Research Group, Department of Cytogenetics and Genome Analysis, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Gatersleben, Germany 06466. corral@ipk-gatersleben.de
authors: Jose M
authors: JM
authors: Corral
authors: Marta Puente
authors: MP
authors: Molins
authors: Olawale M
authors: OM
authors: Aliyu
authors: Timothy F
authors: TF
authors: Sharbel
mesh_terms: D060832:Apomixis / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D056426:Genetic Loci / Q000235:genetics*; D020902:Hypericum / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D018744:DNA, Plant