title: [Construction of recombinant adenovirus of SEA and CD80 genes co-expression regulated by mouse TERT promoter and identification of its expression in hepatoma cells].
abstract: OBJECTIVE
  To construct recombinant co-expression adenovirus vector of SEA and CD80 genes regulated by mouse TERT(telomerase reverse transcriptase, TERT) promoter and to observe the expression of SEA and CD80 in the Hepa1-6 cells mediated by it.
  
  
  METHODS
  Using AdEasy adenovirus system, the core promoter region of mTERT was subcloned to shuttle plasmid pShuttle2 and Myc-Max response element was inserted upstream of it to regulate the expression of SEA and CD80. The recombinant co-expression adenovirus vector of SEA and CD80 genes was constructed and named as Ad-MMRE-mTERT-BIS. Hepatoma cell line Hepa1-6 and fibroblast cell line NIH3T3 were infected by recombinant adenovirus at MOI(multiplicity of infection)of 100, the expression of SEA and CD80 on the surface of cells was detected by indirect immunofluorescent staining.
  
  
  RESULTS
  SEA and CD80 was specifically co-expressed on the surface of infected Hepa1-6 cells but not on NIH3T3 cells.
  
  
  CONCLUSIONS
  The recombinant co-expression adenovirus vector of SEA and CD80 gene regulated by mTERT promoter was sucessfully constructed and make targeting-expression of SEA and CD80 on the surface of hepatoma cells, which lays the foundation for further research on application of SEA and CD80 in targeted genetherapy for hepatoma.
authors: Department of Pathology and Laboratory, No.306 Hospitol of PLA, Beijing, China. sishy306@sohu.com
authors: Shao-yan
authors: SY
authors: Si
authors: Shu-jun
authors: SJ
authors: Song
authors: Bing-xin
authors: BX
authors: Xu
authors: Gang
authors: G
authors: Zhao
authors: Xiao-qing
authors: XQ
authors: Tan
authors: Jun-li
authors: JL
authors: Liu
authors: Jian-zhong
authors: JZ
authors: Zhang
authors: Zhi-guo
authors: ZG
authors: Liu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D045744:Cell Line, Tumor; D004274:DNA, Recombinant / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy; D051379:Mice; D041681:NIH 3T3 Cells; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D020218:Response Elements / Q000235:genetics; D015183:Restriction Mapping; D019098:Telomerase / Q000235:genetics*
chemical_list: D018122:B7-1 Antigen; D004274:DNA, Recombinant; D004768:Enterotoxins; C026066:enterotoxin A, Staphylococcal; D019098:Telomerase