title: Characterization and vaccination of two novel Schistosoma japonicum genes screened from a cercaria cDNA library.
abstract: Two novel genes, SJCWL05 and SJCWL06, were harvested from screening of Schistosoma japonicum (S. japonicum) cercaria cDNA library by using pig sera vaccinated (VPS) with S. japonicum immature egg ws-vaccine (S. japonicum iEw). Prokaryotic recombinant plasmids pGEX-4T-1/SJCWL05 and pGEX-4T-1/SJCWL06 were constructed to analyze their immunogenicity, which was confirmed by SDS-PAGE and Western blotting. Two eukaryotic recombinant plasmids, pcDNA3/SJCWL05 and pcDNA3/SJCWL06, were constructed, and their ability to protect mice against challenge of S. japonicum was evaluated. All mice vaccinated with pcDNA3/SJCWL05 or pcDNA3/SJCWL06 developed ELISA-specific anti-S. japonicum SIEA (S. japonicum soluble immature egg antigens) antibody. Immunoprotection experiments showed that worms and liver eggs reduced 34.64% and 39.14% in the pcDNA3/SJCWL05 group and those reduced 27.17% and 27.95% in the pcDNA3/SJCWL06 group, respectively. The reduction rates of intestine and uterine eggs in female worms of both groups reached 39.45% and 38.5% as well as 30.02% and 28.7%, respectively. Results of our study suggest that novel genes, SJCWL05 and SJCWL06, are potential vaccine candidates against schistosomiasis japonica.
authors: Department of Parasitology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, 410078, People's Republic of China.
authors: Zhi
authors: Z
authors: Tian
authors: Shi-Ping
authors: SP
authors: Wang
authors: Shao-Hua
authors: SH
authors: Zeng
authors: Xue-Qin
authors: XQ
authors: Liu
authors: Dong-Mei
authors: DM
authors: Gao
authors: Qi-Mei
authors: QM
authors: Feng
authors: Shu-Ju
authors: SJ
authors: Zhang
authors: Yun-Fei
authors: YF
authors: Zhou
authors: Xi-Ya
authors: XY
authors: Wang
authors: Ying-Ding
authors: YD
authors: Xia
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D058487:Cercaria / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015723:Gene Library; D006801:Humans; D007422:Intestines / Q000469:parasitology; D051379:Mice; D010270:Parasite Egg Count; D010957:Plasmids / Q000008:administration & dosage; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control*; D014599:Uterus / Q000469:parasitology; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic