title: Distinct hepatic lipid profile of hypertriglyceridemic mice determined by easy ambient sonic-spray ionization mass spectrometry.
abstract: Easy ambient sonic-spray ionization mass spectrometry (EASI-MS) was used to interrogate the hepatic lipid profiles of hypertriglyceridemic and control normotriglyceridemic mice. The analyses of ex vivo complex lipid mixtures were made directly with EASI-MS without accompanying separation steps. Intense ions for phosphatidylcholines and triacylglycerols were observed in the positive ion mode whereas the spectra in the negative ion mode provided profiles of phosphatidylethanolamines and phosphatidylinositol. EASI-MS was coupled to high-performance thin-layer chromatography for analysis of free fatty acids. Fourier transform-ion cyclotron resonance-mass spectrometry was also employed to confirm the identity of the detected lipids. We demonstrated higher incorporation of oleic acid in phosphatidylcholine and triacylglycerol composition, higher relative abundance of arachidonic acid containing phosphatidylinositol, and overall distinct free fatty acid profile in the livers of genetic hypertriglyceridemic mice. We propose that these alterations in liver lipid composition are related to the higher tissue and body metabolic rates described in these hypertriglyceridemic mice.
authors: Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences, University of São Paulo, USP, Ribeirão Preto, São Paulo 14040-903, Brazil. alberici@fcfrp.usp.br
authors: Luciane C
authors: LC
authors: Alberici
authors: Helena C F
authors: HC
authors: Oliveira
authors: Rodrigo R
authors: RR
authors: Catharino
authors: Anibal E
authors: AE
authors: Vercesi
authors: Marcos N
authors: MN
authors: Eberlin
authors: Rosana M
authors: RM
authors: Alberici
mesh_terms: D000818:Animals; D006801:Humans; D015228:Hypertriglyceridemia / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis
chemical_list: D008055:Lipids; D014280:Triglycerides