title: Enhanced production of plasmid DNA by engineered Escherichia coli strains.
abstract: Escherichia coli strains VH33 (PTS⁻ GalP⁺ strain displaying a strongly reduced overflow metabolism) and VH34 (additionally lacking the pyruvate kinase A) were evaluated for the production of a plasmid DNA (pDNA) vaccine. The parent (W3110) and mutant strains were cultured using 10 g of glucose/L. While the specific growth rates of the three strains were similar, they presented differences in the accumulation of acetate. W3110 accumulated up to 4 g/L of acetate, VH33 produced 1.4 g/L, and VH34 only 0.78 g/L. VH33 and VH34 produced 76% and 300% more pDNA than W3110. Moreover, VH34 demanded 33% less oxygen than VH33 and W3110, which can be advantageous for large-scale applications.
authors: Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Artificios No. 40, Col. Miguel Hidalgo, Del. Álvaro Obregón, México DF, CP 01120, Mexico.
authors: Tania E
authors: TE
authors: Pablos
authors: René
authors: R
authors: Soto
authors: Eugenio Meza
authors: EM
authors: Mora
authors: Sylvie
authors: S
authors: Le Borgne
authors: Octavio T
authors: OT
authors: Ramírez
authors: Guillermo
authors: G
authors: Gosset
authors: Alvaro R
authors: AR
authors: Lara
mesh_terms: D000085:Acetates / Q000378:metabolism; D057005:Bioengineering / Q000379:methods*; D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000085:Acetates; D004247:DNA