title: Zinc fixation for flow cytometry analysis of intracellular and surface epitopes, DNA content, and cell proliferation.
abstract: Zinc salt-based fixation (ZBF) is a simple, cost-effective, and nonhazardous fixation method for cell suspensions that preserves all cellular structures and enables flow cytometric analysis of both surface and intracellular proteins, DNA content profiles, and pulse-labeling using the thymidine analog EdU in the same cell sample. ZBF performs equally well to formaldehyde in the preservation of surface epitope labeling and forward and side light scatter parameters, as measured by flow cytometry. DNA is maintained at high molecular weight, improving the quantification and allowing subsequent quantitative PCR analysis. Finally, ZBF treatment allows for long-term storage of labeled cells with little change in these parameters.
authors: Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark.
authors: Rikke
authors: R
authors: Christensen
authors: David M
authors: DM
authors: Owens
authors: Anette
authors: A
authors: Thomsen
authors: Søren
authors: S
authors: Pedersen
authors: Uffe Birk
authors: UB
authors: Jensen
mesh_terms: D049109:Cell Proliferation; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D042541:Intracellular Space / Q000737:chemistry; D016707:Tissue Fixation / Q000379:methods*; D015032:Zinc / Q000737:chemistry*
chemical_list: D000939:Epitopes; D004247:DNA; D015032:Zinc