title: An amide-containing metal-organic tetrahedron responding to a spin-trapping reaction in a fluorescent enhancement manner for biological imaging of NO in living cells.
abstract: Metal-organic polyhedra represent a unique class of functional molecular containers that display interesting molecular recognition properties and fascinating reactivity reminiscent of the natural enzymes. By incorporating a triphenylamine moiety as a bright blue emitter, a robust cerium-based tetrahedron was developed as a luminescent detector of nitronyl nitroxide (PTIO), a specific spin-labeling nitric oxide (NO) trapper. The tetrahedron encapsulates molecules of NO and PTIO within the cavity to prompt the spin-trapping reaction and transforms the normal EPR responses into a more sensitively luminescent signaling system with the limit of detection improved to 5 nM. Twelve-fold amide groups are also functionalized within the tetrahedron to modify the hydrophilic/lipophilic environment, ensuring the successful application of biological imaging in living cells.
authors: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116012, China.
authors: Jian
authors: J
authors: Wang
authors: Cheng
authors: C
authors: He
authors: Pengyan
authors: P
authors: Wu
authors: Jing
authors: J
authors: Wang
authors: Chunying
authors: C
authors: Duan
mesh_terms: D000577:Amides / Q000737:chemistry*; D045744:Cell Line, Tumor; D002563:Cerium / Q000737:chemistry*; D003584:Cytological Techniques / Q000379:methods; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D009569:Nitric Oxide / Q000032:analysis*; D019097:Spin Trapping / Q000379:methods*
chemical_list: D000577:Amides; D049408:Luminescent Agents; D002563:Cerium; D009569:Nitric Oxide