title: Multiple subtypes of serotonin receptors in the feeding circuit of a pond snail.
abstract: In the central nervous system of the pond snail Lymnaea stagnalis, serotonergic transmission plays an important role in controlling feeding behavior. Recent electrophysiological studies have claimed that only metabotropic serotonin (5-HT(2)) receptors, and not ionotropic (5-HT(3)) receptors, are used in synapses between serotonergic neurons (the cerebral giant cells, CGCs) and the follower buccal motoneurons (the B1 cells). However, these data are inconsistent with previous results. In the present study, we therefore reexamined the serotonin receptors to identify the receptor subtypes functioning in the synapses between the CGCs and the B1 cells by recording the compound excitatory postsynaptic potential (EPSP) of the B1 cells evoked by a train of stimulation to the CGC in the presence of antagonists: cinanserin for 5-HT(2) and/or MDL72222 for 5-HT(3). The compound EPSP amplitude was partially suppressed by the application of these antagonists. The rise time of the compound EPSP was longer in the presence of MDL72222 than in that of cinanserin. These results suggest that these two subtypes of serotonin receptors are involved in the CGC-B1 synapses, and that these receptors contribute to compound EPSP. That is, the fast component of compound EPSP is mediated by 5-HT(3)-like receptors, and the slow component is generated via 5-HT(2)-like receptors.
authors: Laboratory of Functional Biology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki 769-2193, Japan.
authors: Ryo
authors: R
authors: Kawai
authors: Suguru
authors: S
authors: Kobayashi
authors: Yutaka
authors: Y
authors: Fujito
authors: Etsuro
authors: E
authors: Ito
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002928:Cinanserin / Q000494:pharmacology; D005247:Feeding Behavior / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D037342:Pertussis Toxin / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000145:classification* / Q000502:physiology*; D012702:Serotonin Antagonists / Q000494:pharmacology; D012908:Snails / Q000502:physiology*; D014326:Tropanes / Q000494:pharmacology
chemical_list: D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D014326:Tropanes; D037342:Pertussis Toxin; D002928:Cinanserin; C042253:bemesetron