title: Isolation and phylogenetic analysis of pandemic H1N1/09 influenza virus from swine in Jiangsu province of China.
abstract: To investigate whether the 2009 pandemic H1N1 influenza A virus was still being transmitted in swine, a total of 1029 nasal swab samples from healthy swine were collected from January to May 2010 in Jiangsu province of China. Eight H1N1 influenza viruses were isolated and identified, and their full length genomes were sequenced. We found that all eight of the H1N1 viruses shared higher than 98.0% sequence identity with the 2009 pandemic virus A/Jiangsu/1/2009 (JS1). In addition, some of these viruses had D225G (3/8) mutations in the receptor binding sites of the hemagglutinin (HA) protein, indicating enhancement of their binding affinity to the sialic α2, 3Gal receptor. In conclusion, the 2009 pandemic H1N1 influenza A virus has retro-infected swine from humans in mainland China, and significant viral evolution is still ongoing in this species.
authors: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
authors: Guo
authors: G
authors: Zhao
authors: Qunping
authors: Q
authors: Fan
authors: Lei
authors: L
authors: Zhong
authors: Yanfang
authors: Y
authors: Li
authors: Wenbo
authors: W
authors: Liu
authors: Xiaowen
authors: X
authors: Liu
authors: Song
authors: S
authors: Gao
authors: Daxin
authors: D
authors: Peng
authors: Xiufan
authors: X
authors: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016679:Genome, Viral / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*