title: Two dimensional difference gel electrophoresis analysis of cerebrospinal fluid in tuberculous meningitis patients.
abstract: Tuberculous meningitis (TBM) is a serious complication of tuberculosis that affects the central nervous system. Present methods to diagnose TBM are not suitable for early diagnosis. Molecular markers and sensitive methods to identify them in the early stage of infection of TBM are critically needed for efficient management. We have done the proteomic analysis of TBM cerebrospinal fluid (n=20) with 2-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. We identified 11 human proteins and 8 mycobacterial proteins with changed expression levels in comparison to controls. Arachidonate 5-lipoxygenase and glial fibrillary acidic protein, two of the identified proteins, were validated with western blot technique on a larger set of disease and control samples (n=40). These two proteins were also analyzed in fungal meningitis samples. We suggest that arachidonate 5-lipoxygenase can be considered for validation as a potential marker for diagnosis of TBM.
authors: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors: Jitender
authors: J
authors: Kataria
authors: Lokesh A
authors: LA
authors: Rukmangadachar
authors: Gururao
authors: G
authors: Hariprasad
authors: Jithesh
authors: J
authors: O
authors: Manjari
authors: M
authors: Tripathi
authors: Alagiri
authors: A
authors: Srinivasan
mesh_terms: D000328:Adult; D001094:Arachidonate 5-Lipoxygenase / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D016919:Meningitis, Cryptococcal / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000235:genetics; D014390:Tuberculosis, Meningeal / Q000134:cerebrospinal fluid / Q000175:diagnosis; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D001094:Arachidonate 5-Lipoxygenase