title: Persistent organic pollutants have dose and CAG repeat length dependent effects on androgen receptor activity in vitro.
abstract: Recently, the effect of exposure to persistent organic pollutants (POPs) on sperm concentration was only seen in men with a short androgen receptor (AR) gene CAG repeat. In order to investigate whether these effects could be observed also in vitro, we tested the impact of 2,2',4,4',5,5'-hexachlorobiphenyl (CB-153) and 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) on 5α-dihydrotestosterone activated ARs containing 16, 22 and 28 CAG repeats, respectively. Single exposure to 4,4'-DDE had the most pronounced effect on the AR activity containing 16 CAG repeats, whereas 28 CAG was the most sensitive variant when a mixture of the two compounds was added. Thus, our in vitro results have confirmed the in vivo data indicating a CAG repeat length dependent effect of endocrine disrupters on the AR activity.
authors: Department of Clinical Sciences, Molecular Genetic Reproductive Medicine, Lund University, Malmö, Sweden. christel.bjork@med.lu.se
authors: Christel
authors: C
authors: Björk
authors: Hannah
authors: H
authors: Nenonen
authors: Aleksander
authors: A
authors: Giwercman
authors: Åke
authors: Å
authors: Bergman
authors: Lars
authors: L
authors: Rylander
authors: Yvonne Lundberg
authors: YL
authors: Giwercman
mesh_terms: D000728:Androgens / Q000494:pharmacology; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003633:Dichlorodiphenyl Dichloroethylene / Q000633:toxicity*; D013196:Dihydrotestosterone / Q000494:pharmacology; D004785:Environmental Pollutants / Q000633:toxicity*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D011944:Receptors, Androgen / Q000235:genetics* / Q000378:metabolism; D018911:Trinucleotide Repeats
chemical_list: D000728:Androgens; D004785:Environmental Pollutants; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D003633:Dichlorodiphenyl Dichloroethylene; D011078:Polychlorinated Biphenyls; C014024:2,4,5,2',4',5'-hexachlorobiphenyl