title: Emblica officinalis exerts antihypertensive effect in a rat model of DOCA-salt-induced hypertension: role of (p) eNOS, NO and oxidative stress.
abstract: Emblica officinalis (EO) has antioxidant properties that could improve redox-sensitive vascular, cardiac and renal changes associated with deoxycorticosterone acetate/1% NaCl high salt (DOCA/HS)-induced hypertension. We determined whether hydroalcoholic lyophilized extract of EO may influence DOCA/HS-induced hypertension by modulating activity of (p) eNOS and endogenous antioxidants. Hypertension was induced in rats by DOCA-salt (20 mg/kg, s.c.) twice weekly for 5 weeks and replacing drinking water with 1% NaCl solution. These rats received cotreatment of different doses of EO (75, 150 and 300 mg/kg/day) for 5 weeks. EO significantly decreased arterial blood pressure and heart rate along with cardiac and renal hypertrophy in a dose-dependent fashion as compared to DOCA control rats. Increased TBARS and decreased endogenous antioxidants including GSH, SOD and GSHPx activity in serum, heart and kidney tissues of hypertensive rats were also normalized. Furthermore, this antihypertensive activity of EO was also linked with increased serum NO, K(+) levels and decreased Na(+) levels. Moreover, EO robustly increased activated eNOS expression in heart. Our results demonstrate that EO reduces oxidative stress, prevents development and progression of hypertension as well as cardiac and renal hypertrophy in DOCA/HS-induced hypertension via modulation of activated eNOS, endogenous antioxidants, serum NO and electrolyte levels.
authors: Department of Pharmacology, All India Institute of Medical Sciences, New Delhi 110029, India. jagriti2012@rediffmail.com
authors: Jagriti
authors: J
authors: Bhatia
authors: Fauzia
authors: F
authors: Tabassum
authors: Ashok Kumar
authors: AK
authors: Sharma
authors: Saurabh
authors: S
authors: Bharti
authors: Mahaveer
authors: M
authors: Golechha
authors: Sujata
authors: S
authors: Joshi
authors: Md
authors: M
authors: Sayeed Akhatar
authors: Abhay Krishna
authors: AK
authors: Srivastava
authors: Dharamvir Singh
authors: DS
authors: Arya
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D006332:Cardiomegaly / Q000201:enzymology / Q000517:prevention & control; D003900:Desoxycorticosterone; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006973:Hypertension / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D007674:Kidney Diseases / Q000201:enzymology / Q000517:prevention & control; D008297:Male; D009569:Nitric Oxide / Q000097:blood*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010766:Phosphorylation; D029842:Phyllanthus emblica; D010936:Plant Extracts / Q000494:pharmacology*; D011188:Potassium / Q000097:blood; D051381:Rats; D017673:Sodium Chloride, Dietary / Q000097:blood; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors
chemical_list: D000959:Antihypertensive Agents; D010936:Plant Extracts; D017673:Sodium Chloride, Dietary; D017392:Thiobarbituric Acid Reactive Substances; D009569:Nitric Oxide; D003900:Desoxycorticosterone; D005979:Glutathione Peroxidase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase; D005978:Glutathione; D011188:Potassium