title: Effects of uranium uptake on transcriptional responses, histological structures and survival rate of the crayfish Procambarus clarkii.
abstract: This work aims to investigate the accumulation levels and effects (transcriptional responses, histopathology and survival rate) associated with a wide range of dissolved uranium (U) concentrations (0, 0.03, 0.6, 4 and 8 mg/L of U) on adult male crayfish Procambarus clarkii during 4 (T4) and 10 (T10) days of exposure. The follow-up of the crayfish mortality showed that P. clarkii was highly resistant to U. Increasing waterborne U concentrations led to increasing bioaccumulation in key crayfish organs and increasing histological damages. U distribution in tissues was also evaluated using transmission electron microscopy and showed the presence of a detoxified form of U in the gill's epithelium in the shape of flakes. Expression levels of mitochondrial genes (cox1, atp6 and 12S gene) and genes involved in oxidative stress (sod(Mn) and mt) were examined together with the housekeeping gene 18S. atp6 and mt genes of P. clarkii were cloned and sequenced before analysis. Significant correlations were observed between U bioaccumulation and the down-regulation of both cox1 and sod(Mn) genes. This work provides a first U toxicogenomic and histopathological pattern of P. clarkii, identify U biomarkers and associate gene expression endpoints to accumulation levels. It also provides new insights into the mechanisms involved in U stress.
authors: Laboratory of Radioecology and Ecotoxicology, Institute of Radioprotection and Nuclear Safety, Bd 186, BP 3, 13115 Saint-Paul-Lez-Durance, France.
authors: Simone
authors: S
authors: Al Kaddissi
authors: Alexia
authors: A
authors: Legeay
authors: Patrice
authors: P
authors: Gonzalez
authors: Magali
authors: M
authors: Floriani
authors: Virginie
authors: V
authors: Camilleri
authors: Rodolphe
authors: R
authors: Gilbin
authors: Olivier
authors: O
authors: Simon
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015415:Biomarkers / Q000032:analysis; D015536:Down-Regulation; D015870:Gene Expression; D050259:Genes, Mitochondrial; D005880:Gills / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D043143:Hepatopancreas / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D018384:Oxidative Stress; D014158:Transcription, Genetic; D014501:Uranium / Q000493:pharmacokinetics*; D014875:Water Pollutants, Radioactive / Q000493:pharmacokinetics*
chemical_list: D015415:Biomarkers; D014875:Water Pollutants, Radioactive; D014501:Uranium