title: Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
abstract: The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. Here we describe a detailed protocol for the reproducible fabrication of PMEDSAH coating on tissue culture polystyrene dishes, and for the feeder-free culture of hES cells on PMEDSAH coating in defined culture medium. This culture system represents a key step toward the fully defined and xenogeneic-free culture of hES cells.
authors: Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
authors: Himabindu
authors: H
authors: Nandivada
authors: Luis G
authors: LG
authors: Villa-Diaz
authors: K Sue
authors: KS
authors: O'Shea
authors: Gary D
authors: GD
authors: Smith
authors: Paul H
authors: PH
authors: Krebsbach
authors: Joerg
authors: J
authors: Lahann
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation; D003094:Collagen; D003470:Culture Media; D004338:Drug Combinations; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007797:Laminin; D008689:Methacrylates / Q000138:chemical synthesis*; D011108:Polymers / Q000138:chemical synthesis*; D011509:Proteoglycans; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis*
chemical_list: D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D008689:Methacrylates; D011108:Polymers; D011509:Proteoglycans; D000644:Quaternary Ammonium Compounds; C568649:poly(2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide); C059485:matrigel; D003094:Collagen