title: Synthesis of functional SiO₂-coated graphene oxide nanosheets decorated with Ag nanoparticles for H₂O₂ and glucose detection.
abstract: In this paper, we report on the first preparation of well-defined SiO(2)-coated graphene oxide (GO) nanosheets (SiO(2)/GO) without prior GO functionalization by combining sonication with sol-gel technique. The functional SiO(2)/GO nanocomposites (F-SiO(2)/GO) obtained by surface functionalization with NH(2) group were subsequently employed as a support for loading Ag nanoparticles (AgNPs) to synthesize AgNP-decorated F-SiO(2)/GO nanosheets (AgNP/F-SiO(2)/GO) by two different routes: (1) direct adsorption of preformed, negatively charged AgNPs; (2) in situ chemical reduction of silver salts. The morphologies of these nanocomposites were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). It is found that the resultant AgNP/F-SiO(2)/GO exhibits remarkable catalytic performance for H(2)O(2) reduction. This H(2)O(2) sensor has a fast amperometric response time of less than 2s. The linear range is estimated to be from 1×10(-4) M to 0.26 M (r=0.998) and the detection limit is estimated to be 4 × 10(-6) M at a signal-to-noise ratio of 3, respectively. We also fabricated a glucose biosensor by immobilizing glucose oxidase (GOD) into AgNP/F-SiO(2)/GO nanocomposite-modified glassy carbon electrode (GCE) for glucose detection. Our study demonstrates that the resultant glucose biosensor can be used for the glucose detection in human blood serum.
authors: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, PR China.
authors: Wenbo
authors: W
authors: Lu
authors: Yonglan
authors: Y
authors: Luo
authors: Guohui
authors: G
authors: Chang
authors: Xuping
authors: X
authors: Sun
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose; D055664:Electrochemical Techniques / Q000379:methods; D005947:Glucose / Q000032:analysis / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D010087:Oxides; D012834:Silver; D012822:Silicon Dioxide; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose