title: Fine depth resolution of two-photon absorption-induced photoacoustic microscopy using low-frequency bandpass filtering.
abstract: Photoacoustic microscopy usually uses high-frequency photoacoustic waves, which provide not only high spatial resolution but also limitation of the penetration depth. In this study, we developed two-photon absorption-induced photoacoustic microscopy (TP-PAM) to improve the depth resolution without use of high-frequency photoacoustic waves. The spatial resolution in TP-PAM is determined by two-photon absorption. TP-PAM with a 20X objective lens (numerical aperture: 0.4) provides an optically-determined depth resolution of 44.9 ± 2.0 μm, which is estimated by the full width at half maximum of the photoacoustic signal from an infinitely small target, using low-frequency bandpass filtering of photoacoustic waves. The combination of TP-PAM and frequency filtering provides high spatial resolution.
authors: Department of Methodologies for Medical Research, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan. yamaoka@koto.kpu-m.ac.jp
authors: Yoshihisa
authors: Y
authors: Yamaoka
authors: Mika
authors: M
authors: Nambu
authors: Tetsuro
authors: T
authors: Takamatsu
mesh_terms: D054459:Elasticity Imaging Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005374:Filtration / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*