title: Hybrid coaxial electrospun nanofibrous scaffolds with limited immunological response created for tissue engineering.
abstract: Electrospinning using synthetic and natural polymers is a promising technique for the fabrication of scaffolds for tissue engineering. Numerous synthetic polymers are available to maximize durability and mechanical properties (polyurethane) versus degradability and cell adhesion (polycaprolactone). In this study, we explored the feasibility of creating scaffolds made of bicomponent nanofibers from both polymers using a coaxial electrospinning system. We used a core of poly(urethane) and a sheath of a mixture of poly(ε-caprolactone) and gelatin, all dissolved in 1,1,1,3,3,3-hexafluror-2-propanol. These nanofibrous scaffolds were then evaluated to confirm their core-sheath nature and characterize their morphology and mechanical properties under static and dynamic conditions. Furthermore, the antigenicity of the scaffolds was studied to confirm that there is no significant foreign body response to the scaffold itself that would preclude its use in vivo. The results show the advantages of combining both natural and synethic polymers to create a coaxial scaffold capable of withstanding dynamic culture conditions and encourage cellular migration to the interior of the scaffold for tissue-engineering applications. Also, the results show that there is no significant immunoreactivity in vivo to the components of the scaffolds.
authors: Department of Surgery, Cardiovascular Tissue Engineering Laboratory, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
authors: Jessica M
authors: JM
authors: Gluck
authors: Paymon
authors: P
authors: Rahgozar
authors: Nilesh P
authors: NP
authors: Ingle
authors: Fironia
authors: F
authors: Rofail
authors: Asdghig
authors: A
authors: Petrosian
authors: Michael G
authors: MG
authors: Cline
authors: Maria C
authors: MC
authors: Jordan
authors: Kenneth P
authors: KP
authors: Roos
authors: William Robb
authors: WR
authors: Maclellan
authors: Richard J
authors: RJ
authors: Shemin
authors: Sepideh
authors: S
authors: Heydarkhan-Hagvall
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000379:methods; D005549:Foreign-Body Reaction / Q000276:immunology*; D005780:Gelatin / Q000737:chemistry; D020007:Implants, Experimental; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D011140:Polyurethanes / Q000737:chemistry; D016062:Porosity; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; C016240:polycaprolactone; D005780:Gelatin