title: Isolation, purification and structure identification of two phenolic glycosides from the roots of Incarvillea younghusbandii Sprague and their antioxidant activities.
abstract: Using a bioassay-guided fractionation technique, two compounds were isolated from the roots of Incarvillea younghusbandii Sprague through silica gel, reverse-phase C18 column chromatography and reverse-phase HPLC. Their structures were identified as acteoside (1) and isoacteoside (2) by ESI-MS, GC-MS, 1D- and 2D-NMR. 1 and 2 showed *OH scavenging capacity similar with benzoic acid, higher O2*- (or *OH) scavenging capacity than ascorbic acid, far higher hepatic LPO inhibitory activities than 2, 6-di-tert-butyl-4-methylphenol (BHT) or ascorbic acid, and more powerful effect on protecting erythrocytes from oxidative damage than ascorbic acid. The *OH scavenging capacity was positively proportional to the concentrations of 1 and 2 ranging from 0.015 6 to 0.500 0 mg x mL(-1). The hepatic LPO inhibitory activities increased with the increasing concentrations of 1 and 2 from 0.001 9 to 0.250 0 mg x mL(-1), but decreased slightly with the increasing concentration from 0.250 0 to 1.0000 mg x L(-1).
authors: Key Laboratory ofBio-resources and Eco-environment, Ministry ofEducation, College of Life Science, Sichuan University, Chengdu 610064, China.
authors: Wei-gao
authors: WG
authors: Pan
authors: Si-ping
authors: SP
authors: Jiang
authors: Peng
authors: P
authors: Luo
authors: Ping
authors: P
authors: Gao
authors: Bin
authors: B
authors: Chen
authors: Hai-tao
authors: HT
authors: Bu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029402:Bignoniaceae / Q000737:chemistry*; D016166:Free Radical Scavengers; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D059808:Polyphenols
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C064683:isoacteoside