title: Transplanted stem cell-secreted vascular endothelial growth factor effects poststroke recovery, inflammation, and vascular repair.
abstract: Cell transplantation offers a novel therapeutic strategy for stroke; however, how transplanted cells function in vivo is poorly understood. We show for the first time that after subacute transplantation into the ischemic brain of human central nervous system stem cells grown as neurospheres (hCNS-SCns), the stem cell-secreted factor, human vascular endothelial growth factor (hVEGF), is necessary for cell-induced functional recovery. We correlate this functional recovery to hVEGF-induced effects on the host brain including multiple facets of vascular repair and its unexpected suppression of the inflammatory response. We found that transplanted hCNS-SCns affected multiple parameters in the brain with different kinetics: early improvement in blood-brain barrier integrity and suppression of inflammation was followed by a delayed spatiotemporal regulated increase in neovascularization. These events coincided with a bimodal pattern of functional recovery, with, an early recovery independent of neovascularization, and a delayed hVEGF-dependent recovery coincident with neovascularization. Therefore, cell transplantation therapy offers an exciting multimodal strategy for brain repair in stroke and potentially other disorders with a vascular or inflammatory component.
authors: Department of Neurosurgery and Stanford Stroke Center, Stanford Institute for Neuro-Innovation and Translational Neurosciences, Stanford University School of Medicine, Stanford, California 94305-5487, USA.
authors: Nobutaka
authors: N
authors: Horie
authors: Marta P
authors: MP
authors: Pereira
authors: Kuniyasu
authors: K
authors: Niizuma
authors: Guohua
authors: G
authors: Sun
authors: Hadar
authors: H
authors: Keren-Gill
authors: Angelo
authors: A
authors: Encarnacion
authors: Mehrdad
authors: M
authors: Shamloo
authors: Scott A
authors: SA
authors: Hamilton
authors: Kewen
authors: K
authors: Jiang
authors: Stephen
authors: S
authors: Huhn
authors: Theo D
authors: TD
authors: Palmer
authors: Tonya M
authors: TM
authors: Bliss
authors: Gary K
authors: GK
authors: Steinberg
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism / Q000502:physiology; D001921:Brain / Q000098:blood supply; D002454:Cell Differentiation; D002478:Cells, Cultured; D002490:Central Nervous System; D006801:Humans; D018919:Neovascularization, Physiologic; D051381:Rats; D011923:Rats, Nude; D020127:Recovery of Function / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000378:metabolism*; D020521:Stroke / Q000628:therapy*; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D042442:Vascular Endothelial Growth Factors