title: Production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid B, from sugarcane juice by Ustilago scitaminea NBRC 32730.
abstract: Mannosylerythritol lipids (MELs) are glycolipid biosurfactants excreted by fungal strains. They show not only excellent surface-active properties but also versatile biochemical actions. Ustilago scitaminea NBRC 32730 has been reported mainly to produce a mono-acetylated and di-acylated MEL, MEL-B, from sucrose as sole carbon source. In order to make biosurfactant production more efficient, we focused our attention on the use of sugarcane juice, one of the most economical resources. The fungal strain produced MEL-B at the yield of 12.7 g/L from only sugarcane juice containing 22.4% w/w sugars. Supplementation with organic (yeast extract, peptone, and urea) and inorganic (sodium nitrate and ammonium nitrate) nitrogen sources markedly enhanced the production yield. Of the nitrogen sources, urea gave the best yield. Under optimum conditions, the strain produced 25.1 g/L of MEL-B from the juice (19.3% sugars) supplemented with 1 g/L of urea in a jar fermenter at 25 °C over 7 d. The critical micelle concentration (CMC) and the surface-tension at the CMC for the present MEL-B were 3.7×10(-6) M and 25.2 mN/m respectively. On water-penetration scan, the biosurfactant efficiently formed the lamella phase (L(α)) and myelins over a wide range of concentrations, indicating excellent surface-active and self-assembling properties. More significantly, the biosurfactant showed a ceramide-like skin-care property in a three-dimensional cultured human skin model. Thus, sugarcane juice is likely to be effective in glycolipid production by U. scitaminea NBRC 32730, and should facilitate the application of MELs.
authors: Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors: Tomotake
authors: T
authors: Morita
authors: Yuko
authors: Y
authors: Ishibashi
authors: Naoto
authors: N
authors: Hirose
authors: Koji
authors: K
authors: Wada
authors: Makoto
authors: M
authors: Takahashi
authors: Tokuma
authors: T
authors: Fukuoka
authors: Tomohiro
authors: T
authors: Imura
authors: Hideki
authors: H
authors: Sakai
authors: Masahiko
authors: M
authors: Abe
authors: Dai
authors: D
authors: Kitamoto
mesh_terms: D002244:Carbon; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry; D015394:Molecular Structure; D009584:Nitrogen; D031786:Saccharum / Q000737:chemistry*; D017592:Skin Care; D013395:Sucrose / Q000737:chemistry; D013500:Surface Tension; D013501:Surface-Active Agents / Q000138:chemical synthesis / Q000737:chemistry*; D014588:Ustilago / Q000737:chemistry* / Q000254:growth & development
chemical_list: D003470:Culture Media; D006017:Glycolipids; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D013395:Sucrose; D002244:Carbon; D009584:Nitrogen