title: Regeneration of irradiated salivary glands with stem cell marker expressing cells.
abstract: BACKGROUND
  Stem cell therapy could be a potential way for reducing radiation-induced hyposalivation and improving the patient's quality of life. However, the identification and purification of salivary gland stem cells have not been accomplished. This study aims to better characterize the stem/progenitor cell population with regenerative potential residing in the mouse salivary gland.
  
  
  METHODS
  Mouse submandibular gland tissue, isolated cells and cultured 3 day old salispheres were tested for their expression of stem cell markers c-Kit, CD133, CD49f, and CD24 using immunohistochemistry for tissue and flow cytometry for cells. Mice were locally irradiated with a single dose of 15 Gy and transplanted with cells expressing defined markers.
  
  
  RESULTS
  Cells expressing known stem cell markers are localized in the larger ducts of the mouse salivary gland. Isolated cells and cells from day 3 salispheres also express these markers: c-Kit (0.058% vs. 0.65%), CD133 (6% vs. 5%), CD49f (78% vs. 51%), and CD24 (60% vs. 60%, respectively). Intraglandular transplantation of these cells into irradiated salivary glands of mice resulted in stem cell marker-specific recovery of salivary gland function.
  
  
  CONCLUSIONS
  Different stem cell-associated markers are expressed in mouse salivary gland cells, which upon transplantation are able to regenerate the irradiation damaged salivary gland.
authors: Department of Cell Biology, Section Radiation and Stress Cell Biology, University of Groningen, The Netherlands.
authors: Lalitha S Y
authors: LS
authors: Nanduri
authors: Martti
authors: M
authors: Maimets
authors: Sarah A
authors: SA
authors: Pringle
authors: Marianne
authors: M
authors: van der Zwaag
authors: Ronald P
authors: RP
authors: van Os
authors: Robert P
authors: RP
authors: Coppes
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D051927:CD24 Antigen / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000378:metabolism; D006258:Head and Neck Neoplasms / Q000532:radiotherapy; D007124:Immunoenzyme Techniques; D039503:Integrin alpha6 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012038:Regeneration; D012469:Salivary Glands / Q000166:cytology* / Q000528:radiation effects*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D014987:Xerostomia / Q000209:etiology / Q000628:therapy
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D006023:Glycoproteins; D039503:Integrin alpha6; D010455:Peptides; C000606024:Prom1 protein, mouse; D019009:Proto-Oncogene Proteins c-kit