title: Intraindividual variability of homocysteine and related thiols concentrations in follicular fluid.
abstract: OBJECTIVE
  To determine intraindividual variability in concentrations of homocysteine and related thiols in follicular fluids of particular follicles after ovarian stimulation and assess the differences between follicles with/or without oocytes.
  
  
  METHODS
  HPLC-FD analysis of plasma and follicular fluid cysteine, homocysteine, cysteinylglycine and glutathione in women undergoing IVF.
  
  
  RESULTS
  In blood plasma, the homocysteine, cysteine, and cysteinylglycine concentrations decreased significantly during stimulation with rec FSH (p<0.001). We found significant differences in concentrations of cysteine and glutathione between follicles with or without retrieved oocytes. High intraindividual variability in concentrations of thiols was determined.
  
  
  CONCLUSIONS
  The concentration variability of thiols between single follicles is very high and we recommend mean at least from 3 follicles with/or without oocytes for characterization of each woman. It is the best to examine individual follicles for further research and analysis of fertility outcomes.
authors: Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, Brno, Czech Republic. mkralik@med.muni.cz
authors: Michaela
authors: M
authors: Kralikova
authors: Jitka
authors: J
authors: Melounova
authors: Igor
authors: I
authors: Crha
authors: Milena
authors: M
authors: Matejovicova
authors: Jana
authors: J
authors: Zakova
authors: Jiri
authors: J
authors: Jarkovsky
authors: Jaroslava
authors: J
authors: Tallova
mesh_terms: D000328:Adult; D003545:Cysteine / Q000097:blood / Q000378:metabolism; D004151:Dipeptides / Q000097:blood / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D006710:Homocysteine / Q000097:blood / Q000378:metabolism*; D006801:Humans; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D013438:Sulfhydryl Compounds; D006710:Homocysteine; C028505:cysteinylglycine; D005978:Glutathione; D003545:Cysteine