title: Analysis of metabolism of 6FDG: a PET glucose transport tracer.
abstract: BACKGROUND
  We are developing (18)F-labeled 6-fluoro-6-deoxy-D-glucose ([(18)F]6FDG) as a tracer of glucose transport. As part of this process it is important to characterize and quantify putative metabolites. In contrast to the ubiquitous positron emission tomography (PET) tracer (18)F-labeled 2-fluoro-2-deoxy-D-glucose ([(18)F]2FDG) which is phosphorylated and trapped intracellularly, the substitution of fluorine for a hydroxyl group at carbon-6 in [(18)F]6FDG should prevent its phosphorylation. Consequently, [(18)F]6FDG has the potential to trace the transport step of glucose metabolism without the confounding effects of phosphorylation and subsequent steps of metabolism. Herein the focus is to determine whether, and the degree to which, [(18)F]6FDG remains unchanged following intravenous injection.
  
  
  METHODS
  Biodistribution studies were performed using 6FDG labeled with (18)F or with the longer-lived radionuclides (3)H and (14)C. Tissues were harvested at 1, 6, and 24 h following intravenous administration and radioactivity was extracted from the tissues and analyzed using a combination of ion exchange columns, high-performance liquid chromatography, and chemical reactivity.
  
  
  RESULTS
  At the 1 h time-point, the vast majority of radioactivity in the liver, brain, heart, skeletal muscle, and blood was identified as 6FDG. At the 6-h and 24-h time points, there was evidence of a minor amount of radioactive material that appeared to be 6-fluoro-6-deoxy-D-sorbitol and possibly 6-fluoro-6-deoxy-D-gluconic acid.
  
  
  CONCLUSIONS
  On the time scale typical of PET imaging studies radioactive metabolites of [(18)F]6FDG are negligible.
authors: Department of Radiology, Case Western Reserve University, Cleveland, OH 44106, USA. raymond.muzic@case.edu
authors: Raymond F
authors: RF
authors: Muzic
authors: Visvanathan
authors: V
authors: Chandramouli
authors: Hsuan-Ming
authors: HM
authors: Huang
authors: Chunying
authors: C
authors: Wu
authors: Yanming
authors: Y
authors: Wang
authors: Faramarz
authors: F
authors: Ismail-Beigi
mesh_terms: D000818:Animals; D001692:Biological Transport; D002851:Chromatography, High Pressure Liquid; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D005260:Female; D005462:Fluorine Radioisotopes; D005947:Glucose / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry; D049268:Positron-Emission Tomography / Q000379:methods*; D011849:Radioactive Tracers; D051381:Rats; D017207:Rats, Sprague-Dawley; D014867:Water / Q000737:chemistry
chemical_list: D005462:Fluorine Radioisotopes; D011849:Radioactive Tracers; D014867:Water; C035308:6-deoxy-6-fluoroglucose; D003847:Deoxyglucose; D005947:Glucose; D000432:Methanol