title: Deep tissue fluorescent imaging in scattering specimens using confocal microscopy.
abstract: In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.
authors: Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA. sgclende@indiana.edu
authors: Sherry G
authors: SG
authors: Clendenon
authors: Pamela A
authors: PA
authors: Young
authors: Michael
authors: M
authors: Ferkowicz
authors: Carrie
authors: C
authors: Phillips
authors: Kenneth W
authors: KW
authors: Dunn
mesh_terms: D000818:Animals; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007668:Kidney / Q000737:chemistry* / Q000166:cytology*; D018613:Microscopy, Confocal / Q000379:methods*; D051381:Rats