title: Biofumigation for control of pale potato cyst nematodes: activity of brassica leaf extracts and green manures on Globodera pallida in vitro and in soil.
abstract: The effects of brassica green manures on Globodera pallida were assessed in vitro and in soil microcosms. Twelve of 22 brassica accessions significantly inhibited the motility of G. pallida infective juveniles in vitro. Green manures of selected brassicas were then incorporated into soil containing encysted eggs of G. pallida. Their effect on egg viability was estimated by quantifying nematode actin 1 mRNA by RT-qPCR. The leaf glucosinolate profiles of the plants were determined by high-performance liquid chromatography. Three Brassica juncea lines (Nemfix, Fumus, and ISCI99) containing high concentrations of 2-propenyl glucosinolate were the most effective, causing over 95% mortality of encysted eggs of G. pallida in polyethylene-covered soil. The toxic effects of green manures were greater in polyethylene-covered than in open soil. Toxicity in soil correlated with the concentration of isothiocyanate-producing glucosinolate but not total glucosinolate in green manures.
authors: Centre for Plant Sciences, University of Leeds , Leeds LS2 9JT, UK.
authors: James S
authors: JS
authors: Lord
authors: Luca
authors: L
authors: Lazzeri
authors: Howard J
authors: HJ
authors: Atkinson
authors: Peter E
authors: PE
authors: Urwin
mesh_terms: D000818:Animals; D001937:Brassica / Q000737:chemistry*; D008372:Manure / Q000032:analysis*; D010572:Pest Control, Biological / Q000379:methods*; D010935:Plant Diseases / Q000469:parasitology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D015800:Protozoan Proteins / Q000235:genetics; D012987:Soil / Q000032:analysis / Q000469:parasitology*; D011198:Solanum tuberosum / Q000469:parasitology*; D014431:Tylenchoidea / Q000187:drug effects* / Q000235:genetics
chemical_list: D008372:Manure; D010936:Plant Extracts; D015800:Protozoan Proteins; D012987:Soil