title: The effects of prenatal stress on expression of CaMK-II and L-Ca2+ channel in offspring hippocampus.
abstract: The purpose of the present study was to characterize the expressions of phosphorylated Ca(2+)/calmodulin-dependent protein kinase II (p-CaMK-II), total CaMK-II, and L-type Ca(2+) channel in offspring hippocampus that was induced by prenatal restraint stress. Pregnant rats were divided into two groups: the control group and the prenatal stress (PNS) group. Pregnant rats in the PNS group were exposed to restraint stress on day 14-20 of pregnancy three times daily for 45 min. Adult offspring rats were used in this study. The results demonstrated that prenatal restraint stress induced a significant increase in the expression of p-CaMK-II, total CaMK-II, and L-Ca(2+) channel by western blot analysis in offspring hippocampus. The immunohistochemistry results revealed that PNS increased the expressions of CaMK-II and L-Ca(2+) channel in the hippocampal CA3 of offspring rats. These data suggest that PNS can have long-term neuronal effects within hippocampal structure involved in the feedback mechanisms of the hypothalamo-pituitary-adrenal axis.
authors: Department of Physiology, Tianjin University of Traditional Medicine, Tianjin 300193, China. Eqingcai@tjutcm.edu.cn
authors: Qing
authors: Q
authors: Cai
authors: Boli
authors: B
authors: Zhang
authors: Shuyun
authors: S
authors: Huang
authors: Tao
authors: T
authors: Wang
authors: Tao
authors: T
authors: Zhou
mesh_terms: D000818:Animals; D015153:Blotting, Western; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D018811:Maternal Exposure; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological
chemical_list: D020746:Calcium Channels, L-Type; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2