title: Improvement of a Venus-based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in living cells.
abstract: Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction.
authors: Laboratory of Molecular Biology and Cell Informatics, Division of Bioscience and Informatics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan. cnakagawa@bioinfo.osakafu-u.ac.jp
authors: Chika
authors: C
authors: Nakagawa
authors: Kazuto
authors: K
authors: Inahata
authors: Shigenori
authors: S
authors: Nishimura
authors: Kenji
authors: K
authors: Sugimoto
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001681:Biological Assay / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D017354:Point Mutation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000737:chemistry*; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000737:chemistry*; D014162:Transfection
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; C054562:yellow fluorescent protein, Bacteria