title: InlB-mediated Listeria monocytogenes internalization requires a balanced phospholipase D activity maintained through phospho-cofilin.
abstract: Internalization of Listeria monocytogenes into non-phagocytic cells is tightly controlled by host cell actin dynamics and cell membrane alterations. However, knowledge about the impact of phosphatidylcholine cleavage driven by host cell phospholipase D (PLD) on Listeria internalization into epithelial cells is limited. Here, we report that L. monocytogenes activates PLD in Vero cells during the internalization. With immunostaining it was shown that both PLD1 and PLD2 surrounded partially or completely the phagocytic cup of most L. monocytogenes. Either up- or down-regulation of PLD expression (activity) diminished Listeria internalization. Both PLD1 and PLD2 in Vero cells were required for efficient Listeria internalization, and could substitute for each other in the regulation of Listeria internalization. Further, exogenous InlB activated host cell PLD1 and PLD2 via the Met receptor, and restored host PLD activation by InlB-deficient L. monocytogenes. InlB-induced PLD activation and Listeria internalization were tightly controlled by phospho-cycling of cofilin. PLD1, but not PLD2, was involved in cofilin-mediated PLD activation and Listeria internalization. These data indicate that cofilin-dependent PLD activation induced by InlB may represent a novel regulation mechanism for efficient Listeria internalization into epithelial cells.
authors: Department for Hospital Infection Control & Research, Institute of Disease Control & Prevention of PLA, Academy of Military Medical Sciences, Beijing, China.
authors: Xuelin
authors: X
authors: Han
authors: Rentao
authors: R
authors: Yu
authors: Lei
authors: L
authors: Ji
authors: Dongyu
authors: D
authors: Zhen
authors: Sha
authors: S
authors: Tao
authors: Shuai
authors: S
authors: Li
authors: Yansong
authors: Y
authors: Sun
authors: Liuyu
authors: L
authors: Huang
authors: Zhe
authors: Z
authors: Feng
authors: Xianping
authors: X
authors: Li
authors: Gaige
authors: G
authors: Han
authors: Martina
authors: M
authors: Schmidt
authors: Li
authors: L
authors: Han
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002522:Chlorocebus aethiops; D004847:Epithelial Cells / Q000382:microbiology; D054884:Host-Pathogen Interactions; D007150:Immunohistochemistry; D008089:Listeria monocytogenes / Q000472:pathogenicity*; D008565:Membrane Proteins / Q000378:metabolism*; D010587:Phagocytosis; D010739:Phospholipase D / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D014709:Vero Cells
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D001426:Bacterial Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; C095768:inlB protein, Listeria monocytogenes; D010739:Phospholipase D