title: Validation of an analytical method for the quantification of pyrethrins on lemons and apricots using high-performance liquid chromatography/mass spectrometry.
abstract: An analytical procedure has been developed for the determination of natural pyrethrins (pyrethrin I, pyrethrin II, jasmolin I, jasmolin II, cinerin I and cinerin II) in lemon and apricot. The QuEChERS method, which stands for quick, easy, cheap, effective, rugged and safe was used for extraction. Analysis of the extract was performed by liquid-chromatography-electrospray ionisation-tandem mass spectrometry. The ions prominent in the ESI spectra were [M+H]+ for the six compounds. A Zorbax SB-C18 column was used with a programmed gradient mobile phase consisting of (A) water containing 0.1% formic acid and 5 mM ammonium formate and (B) ACN. The method was linear within the investigated concentration range, displaying a calibration curve correlation factor of 0.99. The coefficients of variation obtained (RSD) were below 20% and the recoveries were in the 70-110% range.
authors: Instrumental Laboratory, Technological National Center of Canning Industry (CTC), Murcia, Spain.
authors: Irene
authors: I
authors: Ruiz
authors: Ascensión
authors: A
authors: Morales
authors: José
authors: J
authors: Oliva
authors: Alberto
authors: A
authors: Barba
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D027861:Prunus / Q000737:chemistry*; D011722:Pyrethrins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D010573:Pesticide Residues; D011722:Pyrethrins