title: Contribution of hypoxia-inducible factor-1α to transcriptional regulation of vascular endothelial growth factor in bovine developing luteal cells.
abstract: Vascular endothelial growth factor (VEGF)-dependent angiogenesis is crucial for corpus leteum formation and their functional maintenance in mammalian ovaries. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1α-mediated transcriptional activation contributes to the increased expression of VEGF gene in response to hypoxia in the bovine developing luteal cells (LCs). By real-time RT-PCR analysis, VEGF messenger RNA (mRNA) expression was found to significantly increase under hypoxia or treatment with desferrioxamine (DFX), cobalt chloride (CoCl(2)) or even N-carbobenzoxyl-L-leucinyl-L-leucinyl-L-norvalinal (MG-132), while these increased VEGF mRNA expressions could also be blocked by ferrous ammonium sulfate (FAS) or cis-element oligodeoxynucleotide (dsODN) transfection under hypoxia. Further analysis also found that these changes of VEGF mRNA were consistent with HIF-1α expression or HIF-1 activity. Taken together, our results indicate that VEGF is transcriptionally activated by hypoxia through HIF-1α-mediated mechanisms in LCs. This hypoxia-induced transcriptional activation may be one of the important mechanisms mediating the increase of VEGF expression in developing LCs during mammalian corpus leteum formation.
authors: Department of Animal Science, College of Animal Science and Technology, Anhui Science and Technology University, Bengbu, China.
authors: Zhenghong
authors: Z
authors: Zhang
authors: Dingzhong
authors: D
authors: Yin
authors: Zhengchao
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology; D005260:Female; D005296:Ferrous Compounds / Q000494:pharmacology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000502:physiology*; D007976:Leupeptins / Q000494:pharmacology; D008184:Luteal Cells / Q000502:physiology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000502:physiology*; D015533:Transcriptional Activation / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000502:physiology*
chemical_list: D005296:Ferrous Compounds; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007976:Leupeptins; D009838:Oligodeoxyribonucleotides; D000644:Quaternary Ammonium Compounds; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D003035:Cobalt; C038178:ammonium ferrous sulfate; C018021:cobaltous chloride; D003676:Deferoxamine; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde