title: Research report: the effects of hyperbaric oxygen preconditioning on myocardial biomarkers of cardioprotection in patients having coronary artery bypass graft surgery.
abstract: We have previously conducted and reported on the primary endpoint of a clinical study which demonstrated that hyperbaric oxygen (HBO2) preconditioning consisting of two 30-minute intervals of 100% oxygen at 2.4 atmospheres absolute (ATA) prior to coronary artery bypass graft (CABG) surgery leads to an improvement in left ventricular stroke work (LVSW) 24 hours following CABG. In that study, 81 patients were randomized to treatment with HBO2 (HBO2; n = 41) or routine treatment (Control Group; n = 40) prior to surgery. The objective of this manuscript is to further report on the result of the exploratory secondary endpoints from that study, specifically the effects of HBO2 preconditioning on biomarkers of myocardial protection. Intraoperative right atrial biopsies were assessed, via an Enzyme Linked ImmunoSorbent Assay (ELISA), for the expression of eNOS and HSP72. In this study, no significant differences were observed between the groups with respect to the quantity of myocardial eNOS and HSP72. However, in the HBO2 Group, following ischemia and reperfusion, the quantities of myocardial eNOS and HSP72 were increased. This suggests that HBO2 preconditioning in this group of patients may be capable of inducing endogenous cardioprotection following ischemic reperfusion injury (IRI).
authors: Department of Cardiothoracic Surgery, Castle Hill Hospital, Cottingham, United Kingdom. jeysenzy@msn.com
authors: Zivan Yogaratnam
authors: ZY
authors: Jeysen
authors: Laden
authors: L
authors: Gerard
authors: Guvendik
authors: G
authors: Levant
authors: Mike
authors: M
authors: Cowen
authors: Alex
authors: A
authors: Cale
authors: Steve
authors: S
authors: Griffin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D001026:Coronary Artery Bypass; D050884:HSP72 Heat-Shock Proteins / Q000032:analysis*; D006325:Heart Atria / Q000737:chemistry / Q000473:pathology; D006801:Humans; D006931:Hyperbaric Oxygenation; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control; D009206:Myocardium / Q000737:chemistry*; D052250:Nitric Oxide Synthase Type III / Q000032:analysis*; D011446:Prospective Studies; D058028:Research Report; D055815:Young Adult
chemical_list: D015415:Biomarkers; D050884:HSP72 Heat-Shock Proteins; D052250:Nitric Oxide Synthase Type III