title: Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths.
abstract: A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide.
authors: Institute of Advanced Energy, Kyoto University, Uji, Kyoto, Japan.
authors: Shun
authors: S
authors: Nakano
authors: Eiji
authors: E
authors: Nakata
authors: Takashi
authors: T
authors: Morii
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010455:Peptides; C503402:Rev peptide