title: Telomere DNA deficiency is associated with development of human embryonic aneuploidy.
abstract: Aneuploidy represents the most prevalent form of genetic instability found in human embryos and is the leading genetic cause of miscarriage and developmental delay in newborns. Telomere DNA deficiency is associated with genomic instability in somatic cells and may play a role in development of aneuploidy commonly found in female germ cells and human embryos. To test this hypothesis, we developed a method capable of quantifying telomere DNA in parallel with 24-chromosome aneuploidy screening from the same oocyte or embryo biopsy. Aneuploid human polar bodies possessed significantly less telomere DNA than euploid polar bodies from sibling oocytes (-3.07 fold, P = 0.016). This indicates that oocytes with telomere DNA deficiency are prone to aneuploidy development during meiosis. Aneuploid embryonic cells also possessed significantly less telomere DNA than euploid embryonic cells at the cleavage stage (-2.60 fold, P = 0.002) but not at the blastocyst stage (-1.18 fold, P = 0.340). The lack of a significant difference at the blastocyst stage was found to be due to telomere DNA normalization between the cleavage and blastocyst stage of embryogenesis and not due to developmental arrest of embryos with short telomeres. Heterogeneity in telomere length within oocytes may provide an opportunity to improve the treatment of infertility through telomere-based selection of oocytes and embryos with reproductive competence.
authors: Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, United States of America. ntreff@rmanj.com
authors: Nathan R
authors: NR
authors: Treff
authors: Jing
authors: J
authors: Su
authors: Deanne
authors: D
authors: Taylor
authors: Richard T
authors: RT
authors: Scott
mesh_terms: D000782:Aneuploidy; D001755:Blastocyst / Q000378:metabolism; D045744:Cell Line, Tumor; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015894:Genome, Human / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D009865:Oocytes / Q000378:metabolism; D016615:Telomere / Q000235:genetics*