title: A multiplexed immunoassay system based upon reciprocating centrifugal microfluidics.
abstract: A novel, centrifugal disk-based micro-total analysis system (μTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. The described reciprocating mechanism results in a reduction in processing time and reagent consumption by one order of magnitude.
authors: Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway, Irvine, California 92697-3975, USA.
authors: Zahra
authors: Z
authors: Noroozi
authors: Horacio
authors: H
authors: Kido
authors: Régis
authors: R
authors: Peytavi
authors: Rie
authors: R
authors: Nakajima-Sasaki
authors: Algimantas
authors: A
authors: Jasinskas
authors: Miodrag
authors: M
authors: Micic
authors: Philip L
authors: PL
authors: Felgner
authors: Marc J
authors: MJ
authors: Madou
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D019117:Burkholderia; D002498:Centrifugation / Q000295:instrumentation*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007074:Immunoglobulin G / Q000276:immunology; D007202:Indicators and Reagents / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007202:Indicators and Reagents