title: Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice.
abstract: OBJECTIVE
  To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (A.E.), a Chinese medicinal herb, against coxsackievirus B3 (CVB3).
  
  
  METHODS
  The antiviral effects of A.E. against CVB3 in vitro (primarily cultured myocardial cells) and in vivo (BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.
  
  
  RESULTS
  A.E. exhibited obvious antiviral: effects in vivo, and serum samples obtained from the rats with oral administration of A.E. (10 μg/mL, 5 μg/mL), reduced the virus titers in the infected myocardial cells (3.00±0.70, 3.55±0.52, P<0.01). Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% (P<0.01) in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P<0.01). Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.
  
  
  CONCLUSIONS
  Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo.
authors: Institution of Healthy and Toxicology, Beijing Centers for Disease Prevention and Control, Beijing, China.
authors: Ji
authors: J
authors: Pang
authors: Jin-peng
authors: JP
authors: Guo
authors: Min
authors: M
authors: Jin
authors: Zhi-qiang
authors: ZQ
authors: Chen
authors: Xin-wei
authors: XW
authors: Wang
authors: Jun-Wen
authors: JW
authors: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections / Q000097:blood / Q000188:drug therapy* / Q000473:pathology / Q000821:virology*; D004770:Enterovirus / Q000187:drug effects*; D007887:Fabaceae / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D014709:Vero Cells; D019562:Viral Load
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D012333:RNA, Messenger