title: Hepatoprotective effects of saponarin, isolated from Gypsophila trichotoma Wend. on cocaine-induced oxidative stress in rats.
abstract: The antioxidant effect of saponarin, which is the main flavone isolated from Gypsophila trichotoma Wend., and its protection against cocaine hepatotoxicity were investigated in male Wistar rats. The animals were treated with cocaine (40 mg/kg i.p.) alone and also after 3 consecutive days of pretreatment with saponarin (80 mg/kg p.o.). After 18 hours the rats were sacrificed by decapitation. The production of thiobarbituric acid reactive substances, reduced glutathione (GSH) and the activity of the following antioxidant enzymes: catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, and glutathione-S-transferase were assessed in liver homogenate. Administered alone, cocaine induced significant hepatotoxicity manifested with GSH depletion and reduced antioxidant defences. Saponarin pretreatment, however, decreased cocaine toxicity both by increasing GSH levels and antioxidant enzyme activities. The results of this study proved the antioxidant activity of saponarin and its protective effect against cocaine-induced oxidative stress and hepatotoxicity.
authors: Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria. vesselavitcheva@yahoo.com
authors: Vessela
authors: V
authors: Vitcheva
authors: Rumyana
authors: R
authors: Simeonova
authors: Ilina
authors: I
authors: Krasteva
authors: Maya
authors: M
authors: Yotova
authors: Stefan
authors: S
authors: Nikolov
authors: Mitka
authors: M
authors: Mitcheva
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D047310:Apigenin / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D003042:Cocaine / Q000633:toxicity*; D005960:Glucosides / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014662:Vasoconstrictor Agents / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D005960:Glucosides; D014662:Vasoconstrictor Agents; C457771:saponarin; D047310:Apigenin; D002374:Catalase; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D003042:Cocaine