title: Use of host- and bacteria-derived salivary markers in detection of periodontitis: a cumulative approach.
abstract: In the present study, we propose a novel diagnostic approach, using 3 different salivary markers, representing periodontal pathogen burden, inflammation, and tissue degradation, for detecting periodontitis. The salivary concentrations of Porphyromonas gingivalis, interleukin-1β, and matrix metalloproteinase-8, available from salivary specimens of 165 subjects (84 subjects with advanced periodontitis and 81 controls), were calculated together to obtain a cumulative risk score (CRS). In the calculation of CRS, the concentrations of each marker were divided into tertiles, and cumulative sub-score per each subject were calculated by the multiplication of the tertile values. Three CRS groups, indicating the lowest, medium, or highest risk, were formed with the cumulative sub-scores. Logistic regression analysis and ROC curves were performed to study the association of CRS with periodontitis. The results indicate that CRS, calculated from the 3 salivary biomarkers, is associated with advanced periodontitis more strongly than any of the markers individually. CRS offers a novel, non-invasive model for advanced periodontitis risk categorization that is especially useful in large population surveys where a periodontal examination is not feasible.
authors: Institute of Dentistry, University of Turku, Finland. ulvi.gursoy@utu.fi
authors: Ulvi Kahraman
authors: UK
authors: Gursoy
authors: Eija
authors: E
authors: Könönen
authors: Pirkko J
authors: PJ
authors: Pussinen
authors: Taina
authors: T
authors: Tervahartiala
authors: Kati
authors: K
authors: Hyvärinen
authors: Anna L
authors: AL
authors: Suominen
authors: Veli-Jukka
authors: VJ
authors: Uitto
authors: Susanna
authors: S
authors: Paju
authors: Timo
authors: T
authors: Sorsa
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000175:diagnosis* / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D016015:Logistic Models; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D008875:Middle Aged; D016017:Odds Ratio; D016966:Porphyromonas gingivalis / Q000235:genetics*; D012372:ROC Curve; D012307:Risk Factors; D012463:Saliva / Q000378:metabolism* / Q000382:microbiology
chemical_list: D015415:Biomarkers; D053583:Interleukin-1beta; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8