title: Structural characterization and study of immunoenhancing properties of heteroglycan isolated from a somatic hybrid mushroom (PfloVv1aFB) of Pleurotus florida and Volvariella volvacea.
abstract: A water soluble polysaccharide isolated from the hot aqueous extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleurotus florida and Volvariella volvacea was found to consist of d-glucose, d-galactose, and d-mannose in a molar ratio of nearly 4:1:1 and showed macrophage, splenocyte, and thymocyte activation. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: [structure: see text].
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, West Bengal, India.
authors: Sukesh
authors: S
authors: Patra
authors: Kankan K
authors: KK
authors: Maity
authors: Sanjay K
authors: SK
authors: Bhunia
authors: Biswajit
authors: B
authors: Dey
authors: Soumitra
authors: S
authors: Mandal
authors: Tapas K
authors: TK
authors: Maiti
authors: Samir R
authors: SR
authors: Sikdar
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002678:Chimera; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D051379:Mice; D020076:Pleurotus / Q000254:growth & development*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects; D055367:Volvariella / Q000254:growth & development*
chemical_list: D000276:Adjuvants, Immunologic; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose