title: Enantioselective esterification of (R,S)-2-methylalkanoic acid with Carica papaya lipase in organic solvents.
abstract: Isooctane was the best reaction medium for the enantioselective esterification of (R,S)-2-methylalkanoic acid with n-butanol using Carica papaya lipase as catalyst. Increasing linear alkyl-chain length of racemic 2-methylalkanoic acids from ethyl to hexyl increased the enantioselectivity (E) from 2.1 to 98.2 for the esterification of racemic 2-methylalkanoic acids with n-butanol at 35°C. Decreasing reaction temperature from 40 to 20°C increased the enantioselectivity (E) from 14 to 33 for the esterification of racemic 2-methylhexanoic acids with n-butanol. We obtained a maximum enantioselectivity, of E = 24.3, for the enantioselective esterification of racemic 2-methylhexanoic acids with n-butanol in isooctane at water activity 0.33, and at 35°C.
authors: Department of Biotechnology, Southern Taiwan University, Tainan, Taiwan. cs_chang@mail.stut.edu.tw
authors: Chun-Sheng
authors: CS
authors: Chang
authors: Ssu-Ching
authors: SC
authors: Ho
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism*; D029441:Carica / Q000201:enzymology*; D004951:Esterification; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002264:Carboxylic Acids; D012997:Solvents; D020001:1-Butanol; D008049:Lipase