title: [Expression of ADAR1 isoforms in murine acute T-ALL leukemia model].
abstract: This study was purposed to investigate the expression of ADAR1 isoforms of P110 and P150 during the development of murine leukemia. A Notch1 over-expressing murine T cell acute lymphoblastic leukemia model was used to study the expression of ADAR1. BMMNC were isolated at different stages of disease and CD45.2(+)GFP(+) leukemia cells were sorted by flow cytometry at late stage. The expression of ADAR1 was detected by real time quantitative PCR. The results showed that mouse bone marrow cells from both leukemia and control groups expressed P110 and P150. Difference of P110 and P150 mRNA expression were observed during the development of leukemia. The expression of P110 dramatically increased and was significantly higher than that in control group. However, the expression level of P150 in leukemia group decreased stably and reached one-fourth of that in control group at 14 day. Furthermore, similar expression patterns could be detected in sorted CD45.2(+)GFP(+) leukemia cells. It is concluded that the mRNA expressions of P110 and P150 show diverse patterns in the development of leukemia, suggesting that RNA editing mediated by ADAR1 isoforms may play different roles in leukemia.
authors: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors: Cui-Hua
authors: CH
authors: Ma
authors: Chen
authors: C
authors: Tian
authors: Jing-Hui
authors: JH
authors: Chong
authors: Ying-Xu
authors: YX
authors: Shi
authors: Jin-Hong
authors: JH
authors: Wang
authors: Yong-Min
authors: YM
authors: Lin
authors: Jing
authors: J
authors: Xu
authors: Guo-Guang
authors: GG
authors: Zheng
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D015870:Gene Expression; D051379:Mice; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase