title: A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase II from thermophilic bacterium Carboxydothermus hydrogenoformans.
abstract: We established an Na(2)S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO(2) reduction activity, of 16.9 U/mg.
authors: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Japan. tinou@kais.kyoto-u.ac.jp
authors: Takahiro
authors: T
authors: Inoue
authors: Takashi
authors: T
authors: Yoshida
authors: Kei
authors: K
authors: Wada
authors: Takashi
authors: T
authors: Daifuku
authors: Keiichi
authors: K
authors: Fukuyama
authors: Yoshihiko
authors: Y
authors: Sako
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism*; D001419:Bacteria / Q000737:chemistry / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D005285:Fermentation; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism*; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase