title: Simplified sequence-specific oligonucleotide-based polymerase chain reaction protocol to characterize human major histocompatibility complex A*02 and A*24 specificities.
abstract: Major histocompatibility complex (MHC) multimer technology is used in studies of high scientific and clinical interest for the identification, analysis, purification, and adoptive transfer of virus-specific T cells. MHC peptide multimers are usually specific for MHC A*02 or A*24 specificities because both specificities exhibit a high worldwide frequency. However, commercially available typing methods perform complete typing instead of browsing for these prevailing specificities. In this study we demonstrate an easy and accessible polymerase chain reaction-based method to accurately identify A*02 and A*24 samples in a cost-effective way.
authors: Laboratory of Molecular Immuno-Biology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
authors: Sara
authors: S
authors: Ferrando-Martinez
authors: Manuel
authors: M
authors: Leal
authors: María Francisca
authors: MF
authors: González-Escribano
authors: Yolanda
authors: Y
authors: Vega
authors: Ezequiel
authors: E
authors: Ruiz-Mateos
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005787:Gene Frequency; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics* / Q000276:immunology; D058889:Molecular Typing / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D016133:Polymerase Chain Reaction; D055503:Protein Multimerization / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000737:chemistry* / Q000166:cytology / Q000276:immunology
chemical_list: D017931:DNA Primers; D010455:Peptides