title: Association of platelet-SDF-1 with hemodynamic function and infarct size using cardiac MR in patients with AMI.
abstract: OBJECTIVE
  Platelet-derived stromal-cell-derived factor-1 (SDF-1) plays an important role in trafficking hematopoetic progenitor cells for tissue regeneration and neovascularisation. The aim was to evaluate platelet-SDF-1 and CD34(+) progenitor cells in patients with acute myocardial infarction (AMI) compared with hemodynamic function and infarct size using late gadolinium enhancement (LGE) cardiac magnetic resonance (CMR) imaging.
  
  
  METHODS
  We consecutively evaluated 40 patients with AMI, who received coronary angiography for primary coronary intervention. Blood was sampled for flow cytometry to determine mean fluorescence intensity (MFI) of platelet-SDF-1 and for isolation of CD34(+) progenitor cells. 48h and three months after coronary stenting, all patients underwent 1.5T CMR for volumetric assessment and LGE.
  
  
  RESULTS
  Patients with enhanced platelet-SDF-1 expression (median≥68.5MFI) showed a significant amelioration of left ventricular ejection fraction (LVEF) (baseline vs. follow-up mean±SD: 45±6% vs. 56±6%; P=0.018) and of stroke volume (73.1±19.1mL vs. 89.9±21.3mL; P=0.032) at three-month follow-up in contrast to patients with a decreased platelet-SDF-1 expression level (LVEF: 53±8% vs. 56±10%; P=0.267; stroke volume: 85.6±23.1mL vs. 87.4±23.2mL; P=0.803). Inversely, LGE infarct size showed significantly reduced in patients with enhanced platelet-SDF-1 expression at three months (18.9±12mL vs. 6.3±5.1mL; P=0.002) compared to patients with decreased platelet-SDF-1 (12.7±12.7mL vs. 7.6±8.4mL; P=0.156). Time-dependent autocorrelation coefficients shifted for both SV (lag 1: r=-0.368; P=0.001) and the number of CD34(+) cells (lag 1: r=0.633; P=0.001) to a positive autocorrelation (SV; lag 2: r=0.295; P=0.001; CD34(+) cells; lag 2: r=0.287; P=0.001). Patients with increased number of CD34(+) cells (median≥420cells/hpf) showed a significant amelioration of stroke volume in three-month follow-up (83.9±5.3mL vs. 99.4±4.1mL; P=0.020) compared with patients with decreased number of CD34(+) cells (69.3±4.1mL vs. 76.1±3.2mL; P=0.282).
  
  
  CONCLUSIONS
  Platelet-SDF-1 and number of CD34(+) progenitor cells are associated with CMR hemodynamic function in patients with AMI.
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Eberhard-Karls-Universität Tübingen, Germany.
authors: Tobias
authors: T
authors: Geisler
authors: Lisa
authors: L
authors: Fekecs
authors: Thomas
authors: T
authors: Wurster
authors: Amedeo
authors: A
authors: Chiribiri
authors: Andreas
authors: A
authors: Schuster
authors: Eike
authors: E
authors: Nagel
authors: Stephan
authors: S
authors: Miller
authors: Meinrad
authors: M
authors: Gawaz
authors: Konstantinos
authors: K
authors: Stellos
authors: Boris
authors: B
authors: Bigalke
mesh_terms: D015415:Biomarkers / Q000097:blood; D054377:Chemokine CXCL12 / Q000097:blood*; D005260:Female; D006801:Humans; D019028:Magnetic Resonance Imaging, Cine / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000150:complications / Q000175:diagnosis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D018487:Ventricular Dysfunction, Left / Q000097:blood* / Q000175:diagnosis* / Q000209:etiology
chemical_list: D015415:Biomarkers; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12