title: An ultra-high sensitive bioanalytical method for plasma melatonin by liquid chromatography-tandem mass spectrometry using water as calibration matrix.
abstract: For the endogenous substances with an ultra-low level in biological fluids, such as melatonin, the blank biological matrix is obviously not "blank". This problem leads to a serious issue of the bioanalytical methods development and validation by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This work developed and validated an ultra-high sensitive bioanalytical method for plasma melatonin by LC-MS/MS using water as calibration matrix. The lower limit of quantitation of the method was verified to be 1.0 pg/mL and the method exhibited a linear range of 1-5000 pg/mL. Potential matrix effects, accuracy and precision were fully monitored and validated by two complementary quality control approaches respectively using water and the pooled plasma as matrix. The intra-run and inter-run precisions were less than 11.5% and 12.2%, respectively, and the relative error was below ± 13.8% for all of 5 quality control levels. The method was successfully applied to investigate the daytime (8:00 AM-8:00 PM) baseline level of endogenous plasma melatonin, as well as the pharmacokinetic profiles of exogenous melatonin after oral administration in beagle dogs.
authors: Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, China.
authors: An-Qi
authors: AQ
authors: Wang
authors: Bo-Ping
authors: BP
authors: Wei
authors: Yan
authors: Y
authors: Zhang
authors: Yu-Jun
authors: YJ
authors: Wang
authors: Liang
authors: L
authors: Xu
authors: Ke
authors: K
authors: Lan
mesh_terms: D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards; D004285:Dogs; D006801:Humans; D008297:Male; D008550:Melatonin / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards; D014867:Water / Q000032:analysis
chemical_list: D014867:Water; D008550:Melatonin