title: Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation.
abstract: The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.
authors: School of Life Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
authors: Wern-Joo
authors: WJ
authors: Sohn
authors: Hye-In
authors: HI
authors: Jung
authors: Min-A
authors: MA
authors: Choi
authors: Jin-Hyun
authors: JH
authors: Han
authors: Gi-Jeong
authors: GJ
authors: Gwon
authors: Hitoshi
authors: H
authors: Yamamoto
authors: Sanggyu
authors: S
authors: Lee
authors: Zae Young
authors: ZY
authors: Ryoo
authors: Eui-Kyun
authors: EK
authors: Park
authors: Hong-In
authors: HI
authors: Shin
authors: Han-Sung
authors: HS
authors: Jung
authors: Jae-Young
authors: JY
authors: Kim
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014059:Tongue / Q000166:cytology* / Q000378:metabolism*
chemical_list: C496464:Fgf10 protein, mouse; D051526:Fibroblast Growth Factor 10; D051497:Receptor, Fibroblast Growth Factor, Type 2