title: [Analysis for post-transplant changes of peripheral lymphocyte subsets in liver transplant receptors with HBV infection].
abstract: OBJECTIVE
  To investigate the characters and changes of peripheral lymphocyte subsets and cytokines in liver transplant receptors with HBV infection in short phases after liver transplantation, and to provide evidences for monitoring post-transplant immune condition of liver transplant receptors.
  
  
  METHODS
  Peripheral lymphocyte subsets and cytokine levels in pre- and post- transplant 12 h, 3 d, 10 d, 30 d, 60 d of 20 cases of patients with HBV-associated severe hepatic diseases were investigated and analyzed, and were compared respectively with those of 22 cases of healthy adults as control (HC) with flow cytometry (FCM) and ELISA.
  
  
  RESULTS
  The patients' accounts of peripheral lymphocyte subsets before liver transplantation were lower than those of HC significantly, but the accounts decreased significantly after transplantation 12 h. Three days later, the accounts of lymphocyte subsets increased significantly. The percentages of CD3, CD4, CD8 and NK cells got to stable stage from post-transplantation 10 d, and the absolute accounts of post-transplantation 60 d were higher than those of pre-transplantation, but were still lower than those of HC; The IFN-γ and IL-10 levels of post-transplantation 12 h increased several times and decreased after 3 days. The IL-10 levels in post-transplantation 60 d were still higher than those of HC.
  
  
  CONCLUSIONS
  The absolute accounts of peripheral lymphocyte subsets increased to stable levels from post-transplantation 10 d, but were still lower than those of HC; Post-transplant immune condition was to Th2 polarization.
authors: No.302 Hospital of PLA, Beijing, China. fengyanqing68@sohu.com
authors: Yan-qing
authors: YQ
authors: Feng
authors: Wei-wei
authors: WW
authors: Chen
authors: Jia
authors: J
authors: Liu
authors: Zhen-hong
authors: ZH
authors: Liu
authors: Jun-lian
authors: JL
authors: Yang
authors: Ning
authors: N
authors: Yang
authors: Ping
authors: P
authors: Lv
authors: Yuan-li
authors: YL
authors: Mao
mesh_terms: D000328:Adult; D000368:Aged; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006509:Hepatitis B / Q000276:immunology* / Q000378:metabolism / Q000601:surgery*; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period; D013997:Time Factors
chemical_list: D016207:Cytokines