title: Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "dehalococcoides" strains.
abstract: A microbial consortium that reductively dechlorinates trichloroethene, cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with methanogenesis was enriched from chloroethene-contaminated soil from Japan. Dechlorination activity was maintained for over 4 years. Using quantitative polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were detected. Their growth and dechlorination activities were classified into two types: one that grows by converting cis-DCE to ethene and the other that grows by converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC reductive dehalogenases were detected. Inhibitors of methanogenesis (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of cis-DCE and of VC respectively. These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides."
authors: Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Hakozaki, Fukuoka, Japan.
authors: Taiki
authors: T
authors: Futagami
authors: Fuyuki
authors: F
authors: Okamoto
authors: Hiroshi
authors: H
authors: Hashimoto
authors: Kotaro
authors: K
authors: Fukuzawa
authors: Koji
authors: K
authors: Higashi
authors: K H M Nazmul Hussain
authors: KH
authors: Nazir
authors: Erina
authors: E
authors: Wada
authors: Akiko
authors: A
authors: Suyama
authors: Kaoru
authors: K
authors: Takegawa
authors: Masatoshi
authors: M
authors: Goto
authors: Kanji
authors: K
authors: Nakamura
authors: Kensuke
authors: K
authors: Furukawa
mesh_terms: D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods; D005030:Ethylenes / Q000032:analysis / Q000378:metabolism; D054879:Halogenation / Q000502:physiology; D008697:Methane / Q000032:analysis / Q000378:metabolism; D019638:Methanomicrobiaceae / Q000254:growth & development; D059013:Microbial Consortia; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012988:Soil Microbiology; D013045:Species Specificity; D014241:Trichloroethylene / Q000032:analysis* / Q000378:metabolism; D014752:Vinyl Chloride / Q000032:analysis / Q000378:metabolism
chemical_list: D005030:Ethylenes; D012336:RNA, Ribosomal, 16S; D014241:Trichloroethylene; C036216:ethylene; D008697:Methane; D014752:Vinyl Chloride