title: [Proteomic analysis between keloid and normal skin].
abstract: OBJECTIVE
  To investigate and search correlative proteins of keloid by comparing the results of differential proteomic analysis between keloid and normal skin.
  
  
  METHODS
  From January 2010 to June 2010 two-dimensional gel electrophoresis was used to define patterns of protein expression in keloid skin from 8 patients and matched normal skin from 3 patients. Differential expression protein spots were showed and analyzed by matrix-assisted laser desorption ionization-time of flying/time of flying (MALDI-TOF/TOF) mass spectrometry.
  
  
  RESULTS
  This study succeeded to provide a two-dimensional protein profiling comparison between normal skin and keloid. Gel-analysis software identified an average of 2978 spots in keloid while 3053 spots in normal skin and statistical filtering yielded 40 spots of a 4-fold change, 32 of which were identified by using mass spectrometry, 20 were up-regulated and 12 were down-regulated. Functional analysis revealed that these proteins could be fractionated to carrier proteins (3 proteins), signal transduction proteins (4 proteins), proliferation and apoptosis related proteins (2 proteins), cytoskeleton proteins (6 proteins), extracellular matrix proteins (8 proteins), immunity related proteins (3 proteins), tumor related proteins (2 proteins), and function unknown protein (4 proteins).
  
  
  CONCLUSIONS
  Proteomic analysis can identify the proteins with variance of keloid versus normal skin. The further research to these differential proteins may help reveal the pathogenesis of keloid and provide new treatments for keloid.
authors: Department of Burns, the First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
authors: Bing
authors: B
authors: Tang
authors: Zhi-cheng
authors: ZC
authors: Hu
authors: Bin
authors: B
authors: Zhu
authors: Bin
authors: B
authors: Chen
authors: Kai
authors: K
authors: Zhang
authors: Kun-hua
authors: KH
authors: Hu
authors: Ming-tao
authors: MT
authors: Li
authors: Jia-yuan
authors: JY
authors: Zhu
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D007627:Keloid / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D012867:Skin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D011506:Proteins; D020543:Proteome