title: Genome-wide identification of Chlamydia trachomatis antigens associated with tubal factor infertility.
abstract: OBJECTIVE
  To identify Chlamydia trachomatis antigens that can be used to differentially diagnose tubal factor infertility in comparison with previously reported heat shock protein 60.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  Academic medical center.
  
  
  METHODS
  Infertile women with and without tubal pathology diagnosed laparoscopically.
  
  
  METHODS
  None.
  
  
  METHODS
  Antibody responses to C. trachomatis in infertile women with or without tubal pathologies using a C. trachomatis genome-wide proteome array.
  
  
  RESULTS
  Comparison of the antibody profiles revealed 30 C. trachomatis antigens that were preferentially recognized in women with tubal factor infertility, with a detection sensitivity and specificity of 80.6% and 56.5%, respectively, 10 of which showed 100% specificity. A combination of CT443 and CT381 antigens yielded the highest detection sensitivity (67.7%) while maintaining 100% specificity.
  
  
  CONCLUSIONS
  These findings have demonstrated that antibodies to CT443 and CT381, when used in combination, have higher sensitivity and specificity in predicting tubal factor infertility than other indicators for tubal factor infertility, such as heat shock protein 60 antibodies (35.5%, 100%) or hysterosalpingogram (65%, 83%). Using a panel of C. trachomatis antigens to serologically diagnose tubal factor infertility can save the patients from undertaking expensive and invasive procedures for determining tubal pathology and choosing treatment plans.
authors: Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
authors: Allison K
authors: AK
authors: Rodgers
authors: Nicole M
authors: NM
authors: Budrys
authors: Siqi
authors: S
authors: Gong
authors: Jie
authors: J
authors: Wang
authors: Alan
authors: A
authors: Holden
authors: Robert S
authors: RS
authors: Schenken
authors: Guangming
authors: G
authors: Zhong
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology; D018834:Chaperonin 60 / Q000235:genetics; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000235:genetics*; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000276:immunology; D005187:Fallopian Tubes / Q000382:microbiology / Q000473:pathology; D005260:Female; D055106:Genome-Wide Association Study; D006367:HeLa Cells; D006801:Humans; D007047:Hysterosalpingography; D007247:Infertility, Female / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*; D010535:Laparoscopy; D040901:Proteomics; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018834:Chaperonin 60