title: Analysis of laser-microdissected prostate cancer tissues reveals potential tumor markers.
abstract: Prostate cancer (PCA) is a clinically heterogeneous and often multifocal disease with a clinical outcome difficult to predict. A deeper knowledge of the molecular basis of the disease may lead to a better prediction of prognosis. Therefore, in this study we investigated the molecular basis of PCA by identifying potential tumor markers in laser-microdisected PCA tissues. Among a group of PCA patients, quantitative RT-PCR analysis was performed to compare the expression of 70 genes. These genes were selected from the results of two microarrays which investigated the gene expression profile differences between moderately or poorly differentiated prostate carcinoma glands and the corresponding normal glands. Among the genes examined, CDKN2A, GATA3, CREBBP, ITGA2, NBL1 and TGM4 were down-regulated in the prostate carcinoma glands compared to the corresponding normal glands, whereas TFF3, TMPRSS2 and ERG were up-regulated. Our findings indicate that these genes may play roles as tumor suppressor genes or oncogenes in PCA, and may serve as potential tumor markers and novel therapeutic targets.
authors: Institute of Pathology, University Hospital Bonn, D-53127 Bonn, Germany.
authors: David
authors: D
authors: Adler
authors: Andreas
authors: A
authors: Lindstrot
authors: Reinhard
authors: R
authors: Buettner
authors: Nicolas
authors: N
authors: Wernert
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D066298:In Vitro Techniques; D061346:Laser Capture Microdissection / Q000379:methods*; D008297:Male; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D000071230:Transcriptional Regulator ERG; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism; D000071165:Trefoil Factor-3
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; C072312:ERG protein, human; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C490087:NBL1 protein, human; D010455:Peptides; D011506:Proteins; C082495:TFF3 protein, human; D015534:Trans-Activators; D000071230:Transcriptional Regulator ERG; D000071165:Trefoil Factor-3; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; C418702:transglutaminase 4; D011503:Transglutaminases; D012697:Serine Endopeptidases; C421305:TMPRSS2 protein, human