title: Isoegomaketone induces apoptosis through caspase-dependent and caspase-independent pathways in human DLD1 cells.
abstract: Isoegomaketone (IK) is an essential oil component of Perilla frutescens (L.), but the mechanism by which IK induces apoptosis has never been studied. The purpose of this study was to elucidate the IK-induced apoptotic pathway in DLD1 human colon cancer cells. We observed that IK treatment over 24 h significantly inhibited cell viability in a dose-dependent manner. We also found that IK triggered cleavage of PARP. Moreover, IK treatment resulted in cleavage of caspase-8, -9, and -3 in a dose- and time-dependent manner. IK treatment also resulted in cleavage of Bid and translocation of Bax, and triggered the release of cytochrome c from the mitochondria to the cytoplasm. Furthermore, it resulted in the translocation of apoptosis inducing factor (AIF), a caspase-independent mitochondrial apoptosis factor, from the mitochondria into the nucleus. Overall, these results suggest that IK induces apoptosis through caspase-dependent and capase-independent pathways in DLD1 cells.
authors: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk, Republic of Korea.
authors: Byoung Ok
authors: BO
authors: Cho
authors: Chang Hyun
authors: CH
authors: Jin
authors: Yong Dae
authors: YD
authors: Park
authors: Hyung Won
authors: HW
authors: Ryu
authors: Myung Woo
authors: MW
authors: Byun
authors: Kwon Il
authors: KI
authors: Seo
authors: Il Yun
authors: IY
authors: Jeong
mesh_terms: D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000378:metabolism*; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D020169:Caspases / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005663:Furans / Q000008:administration & dosage* / Q000737:chemistry*; D006801:Humans; D007659:Ketones / Q000008:administration & dosage* / Q000737:chemistry*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D051033:Apoptosis Inducing Factor; D051023:BH3 Interacting Domain Death Agonist Protein; D005663:Furans; D007659:Ketones; D010938:Plant Oils; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; C568138:isoegomaketone; D045304:Cytochromes c; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D020169:Caspases