title: [Effects of Bushenguchiwan on expression of matrix metalloproteinase-13 in rats' periodontium].
abstract: OBJECTIVE
  To investigate the influence of Bushenguchiwan on expression of matrix metalloproteinase-13 (MMP-13) in periodontium of rats with experimental periodontitis.
  
  
  METHODS
  The model of experimental periodontitis of rats was established and treated by Bushenguchiwan with different doses. The periodontal tissues from groups of different doses were immunohistochemically stained by antibody of MMP-13. The expression of MMP-13 was examined and semi-quantitative analysis of signals performed by integrated absorbance.
  
  
  RESULTS
  MMP-13 was intensely positive in gingival epithelial cells and periodontal fibroblasts in periodontitis models and negative in normal rat periodontal tissues. After 30 days of Bushenguchiwan treatment with high dose, middle dose and low dose, the expression of MMP-13 (2.9103 ± 0.5534, 3.6588 ± 0.4330, 4.4550 ± 0.4255) was down-regulated respectively compared with model rats (5.3233 ± 0.7993), P < 0.05. After 60 days of treatment the expression of MMP-13 (2.1855 ± 0.5381, 2.8558 ± 0.4759, 3.8980 ± 0.5885) was down-regulated more significantly. with model rats (6.2693 ± 0.4538), P < 0.05.
  
  
  CONCLUSIONS
  Bushenguchiwan could down-regulate the expression of MMP-13 in rats' periodontium and the high dose group had better effect.
authors: Department of Periodontology, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
authors: Qin
authors: Q
authors: Wen
authors: Heng
authors: H
authors: Yang
authors: Yi
authors: Y
authors: Ding
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D004847:Epithelial Cells / Q000201:enzymology; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D005881:Gingiva / Q000166:cytology / Q000201:enzymology; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism*; D010518:Periodontitis / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology; D010519:Periodontium / Q000166:cytology / Q000201:enzymology*; D016966:Porphyromonas gingivalis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat