title: Deferoxamine affects heat shock protein expression in heart after intracerebral hemorrhage in aged rats.
abstract: Cardiac dysfunction can occur after intracerebral hemorrhage (ICH). This study examined the expression of heat shock proteins (HSPs) in the heart after ICH in aged rats and whether deferoxamine (DFX), an iron chelator, affects HSP expression. Male Fischer 344 rats (18 months old) received an injection of 100 μl autologous blood into the right caudate, whereas sham-operated rats had a needle insertion. The rats were treated with DFX or vehicle at 2 and 6 h after ICH and then every 12 h for 3 days. Rats were killed 3 days after ICH, and the hearts were sampled for Western blot analysis of HSP-27 and HSP-32. Western blotting showed that levels of HSP-32 were reduced in the heart after ICH (p<0.05), and this reduction was normalized by DFX (p<0.05). DFX had no effects on heart HSP-32 levels in sham-operated rats. ICH also resulted in a reduction in HSP-27 (p<0.05), but DFX treatment reduced HSP-27 further (p<0.05). In addition, DFX reduced HSP-27 levels in sham rats. In conclusion, ICH causes HSP-27 and -32 reductions in the heart of aged rats. Deferoxamine treatment has different effects on the expression of HSP-27 and HSP-32.
authors: Department of Neurosurgery, University of Michigan, Ann Arbor, MI 48109-2200, USA.
authors: Hua
authors: H
authors: Hu
authors: Lin
authors: L
authors: Wang
authors: Masanobu
authors: M
authors: Okauchi
authors: Richard F
authors: RF
authors: Keep
authors: Guohua
authors: G
authors: Xi
authors: Ya
authors: Y
authors: Hua
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000473:pathology*; D003676:Deferoxamine / Q000494:pharmacology* / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000187:drug effects*; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D017262:Siderophores / Q000494:pharmacology* / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D055551:HSP27 Heat-Shock Proteins; D017262:Siderophores; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D003676:Deferoxamine