title: Fetal alcohol exposure leads to abnormal olfactory bulb development and impaired odor discrimination in adult mice.
abstract: BACKGROUND
  Children whose mothers consumed alcohol during pregnancy exhibit widespread brain abnormalities and a complex array of behavioral disturbances. Here, we used a mouse model of fetal alcohol exposure to investigate relationships between brain abnormalities and specific behavioral alterations during adulthood.
  
  
  RESULTS
  Mice drank a 10% ethanol solution throughout pregnancy. When fetal alcohol-exposed offspring reached adulthood, we used high resolution MRI to conduct a brain-wide screen for structural changes and found that the largest reduction in volume occurred in the olfactory bulbs. Next, we tested adult mice in an associative olfactory task and found that fetal alcohol exposure impaired discrimination between similar odors but left odor memory intact. Finally, we investigated olfactory bulb neurogenesis as a potential mechanism by performing an in vitro neurosphere assay, in vivo labeling of new cells using BrdU, and in vivo labeling of new cells using a transgenic reporter system. We found that fetal alcohol exposure decreased the number of neural precursor cells in the subependymal zone and the number of new cells in the olfactory bulbs during the first few postnatal weeks.
  
  
  CONCLUSIONS
  Using a combination of techniques, including structural brain imaging, in vitro and in vivo cell detection methods, and behavioral testing, we found that fetal alcohol exposure results in smaller olfactory bulbs and impairments in odor discrimination that persist into adulthood. Furthermore, we found that these abnormalities in olfactory bulb structure and function may arise from deficits in the generation of new olfactory bulb neurons during early postnatal development.
authors: Neurosciences and Mental Health, Hospital for Sick Children, and Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
authors: Katherine G
authors: KG
authors: Akers
authors: Steven A
authors: SA
authors: Kushner
authors: Ana T
authors: AT
authors: Leslie
authors: Laura
authors: L
authors: Clarke
authors: Derek
authors: D
authors: van der Kooy
authors: Jason P
authors: JP
authors: Lerch
authors: Paul W
authors: PW
authors: Frankland
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002648:Child; D004192:Discrimination, Psychological / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000473:pathology / Q000503:physiopathology; D005333:Fetus / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D055495:Neurogenesis / Q000187:drug effects; D009483:Neuropsychological Tests; D009812:Odorants; D009830:Olfactory Bulb / Q000002:abnormalities* / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012903:Smell / Q000187:drug effects*
chemical_list: D000431:Ethanol