title: Iron(III) mesoporphyrin IX and iron(III) deuteroporphyrin IX bind to the Porphyromonas gingivalis HmuY hemophore.
abstract: Porphyromonas gingivalis acquires heme through an outer-membrane heme transporter HmuR and heme-binding hemophore-like lipoprotein HmuY. Here, we compare binding of iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) to HmuY with that of iron(III) protoporphyrin IX (protoheme) and protoporphyrin IX (PPIX) using spectroscopic methods. In contrast to PPIX, mesoheme and deuteroheme enter the HmuY heme cavity and are coordinated by His134 and His166 residues in a fully analogous way to protoheme binding. However, in the case of deuteroheme two forms of HmuY-iron porphyrin complex were observed differing by a 180° rotation of porphyrin about the α-γ-meso-carbon axis. Since the use of porphyrins either as active photosensitizers or in combination with antibiotics may have therapeutic value for controlling bacterial growth in vivo, it is important to compare the binding of heme derivatives to HmuY.
authors: University of Wrocław, Department of Chemistry, F. Joliot-Curie 14 Street, 50-383 Wrocław, Poland.
authors: Jacek
authors: J
authors: Wojaczyński
authors: Halina
authors: H
authors: Wójtowicz
authors: Marcin
authors: M
authors: Bielecki
authors: Mariusz
authors: M
authors: Olczak
authors: John W
authors: JW
authors: Smalley
authors: Lechosław
authors: L
authors: Latos-Grażyński
authors: Teresa
authors: T
authors: Olczak
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003905:Deuteroporphyrins / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D008074:Lipoproteins / Q000737:chemistry* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics; D008652:Mesoporphyrins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D016966:Porphyromonas gingivalis; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D003905:Deuteroporphyrins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D008652:Mesoporphyrins; D006418:Heme; C028839:deuteroporphyrin-IX; C031468:mesoporphyrin IX