title: Polymerase chain reaction confirmation of Babesia canis canis and Anaplasma phagocytophilum in dogs suspected of babesiosis in Slovakia.
abstract: Canine babesiosis was considered an imported tick transmitted disease until the first case of autochthonous canine babesiosis in Slovakia was described in 2002. Since then, the number of cases kept increasing every year. The causative agent of babesiosis in dogs is not yet characterized; therefore, the aim of our study was to determine the agent and the rate of infection in the vector tick D. reticulatus in Slovakia. Babesia canis canis was detected in 80 out of 87 blood samples from dogs with clinical manifestations of babesiosis. Six dogs suspected of babesiosis tested positive for presence of Anaplasma phagocytophilum, and one mixed infection of B. c. canis and A. phagocytophilum was detected. B. c. canis was detected in 35.6% questing adults of D. reticulatus. The obtained sequences from blood samples showed 99.7% and from D. reticulatus, 99.4% similarity with the B. c. canis (AY072926) from dogs infected in Croatia. In our study, we characterized the agent of canine babesiosis from blood samples of naturally infected dogs and D. reticulatus, the vector tick. Further, the presence of A. phagocytophilum, bacterium responsible for the canine granulocytic anaplasmosis, was recorded in dogs for the first time in Slovakia.
authors: Institute of Parasitology, SAS, Hlinkova 3, Kosice, Slovakia. majlat@saske.sk
authors: Viktória
authors: V
authors: Majláthová
authors: Igor
authors: I
authors: Majláth
authors: Bronislava
authors: B
authors: Víchová
authors: Ivana
authors: I
authors: Gul'ová
authors: Markéta
authors: M
authors: Derdáková
authors: Edina
authors: E
authors: Sesztáková
authors: Branislav
authors: B
authors: Pet'ko
mesh_terms: D041081:Anaplasma phagocytophilum / Q000302:isolation & purification; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D030561:Databases, Nucleic Acid; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology* / Q000635:transmission*; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012337:RNA, Ribosomal, 18S; D018154:Slovakia / Q000453:epidemiology; D013987:Ticks / Q000469:parasitology*
chemical_list: D012337:RNA, Ribosomal, 18S