title: Nocardioides daejeonensis sp. nov., a denitrifying bacterium isolated from sludge in a sewage-disposal plant.
abstract: Strain MJ31(T), a gram-reaction-positive, aerobic, rod-shaped, non-motile bacterium, was isolated from a sludge sample collected at the Daejeon sewage-disposal plant, in South Korea, and characterized in order to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MJ31(T) belonged to the genus Nocardioides, appearing most closely related to Nocardioides dubius KSL-104(T) (98.6 % sequence similarity), Nocardioides jensenii DSM 20641(T) (97.6 %), Nocardioides daedukensis MDN22(T) (97.2 %) and Nocardioides mesophilus MSL-22(T) (97.0 %). The chemotaxonomic properties of strain MJ31(T) were consistent with those of the genus Nocardioides: MK-8(H(4)) was the predominant menaquinone, iso-C(16 : 0), iso-C(17 : 0) and C(18 : 1)ω9c were the predominant cellular fatty acids, and the cell-wall peptidoglycan was based on LL-2,6-diaminopimelic acid. The genomic DNA G+C content of strain MJ31(T) was 71.2 mol%. Some differential phenotypic properties and low DNA-DNA relatedness values (<28 %) with the type strains of closely related species indicated that strain MJ31(T) represents a novel species, for which the name Nocardioides daejeonensis sp. nov. is proposed. The type strain is MJ31(T) ( = KCTC 19772(T) = JCM 16922(T)).
authors: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors: Sung-Geun
authors: SG
authors: Woo
authors: Department of Bio and Environmental Technology, Division of Environmental and Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors: Sathiyaraj
authors: S
authors: Srinivasan
authors: School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.|Research and Development Division, H-Plus Eco Ltd, BVC 301, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors: Jihoon
authors: J
authors: Yang
authors: Korea Testing Certification, Chemical Industry Team, 587-10, Sinsa-Dong, Gangnam-Gu, Seoul, Republic of Korea.
authors: Yong-An
authors: YA
authors: Jung
authors: Department of Bio and Environmental Technology, Division of Environmental and Life Science, College of Natural Science, Seoul Women's University, 623 Hwarangno, Nowon-gu, Seoul 139-774, Republic of Korea.
authors: Myung Kyum
authors: MK
authors: Kim
authors: Research and Development Division, H-Plus Eco Ltd, BVC 301, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
authors: Myungjin
authors: M
authors: Lee
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058440:Denitrification; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; D003960:Diaminopimelic Acid