title: Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation.
abstract: Two polymorphic forms of an extracellular arabinogalactan protein (AGP1 and AGP2), obtained from the conditioned media of two carrot suspension-cultured cell lines, have been identified in terms of binding of the anti-plasma membrane antibodies JIM4 and MAC207. AGP1 and AGP2 have been used as immunogens to generate further anti-AGP monoclonal antibodies. JIM14 identified an epitope carried by AGP2 and also by glycoproteins of low molecular weight localized to the plant cell wall. In addition, further antibodies (JIM13 and JIM15) identified carbohydrate epitopes of the AGPs that also occur on plasma membrane glycoproteins and are expressed by patterns of cells that reflect cell position at the carrot root apex. Indirect immunofluorescence microscopy indicated that JIM13 recognized the surface of cells forming the epidermis and cells marking the region and axis of the future xylem. JIM15 recognized a pattern of cells directly complementary to the JIM13 pattern. The panel of anti-AGP monoclonal antibodies now available indicates groups of cells within the root meristem that may reflect an early pre-pattern of the tissues of the mature root structure and suggests extensive modulation of cell surface AGPs during cell development and the positioning of cells within the apex.
authors: For correspondance: centre for Plant Biochemistry and Biotechnology, Unversity of Leeds, Leeds LS2 9JT, UK (fax +44 532 333167)Department of Cell Biology, AFRC institute of Plant Science Research, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors: J P
authors: JP
authors: Knox
authors: For correspondance: centre for Plant Biochemistry and Biotechnology, Unversity of Leeds, Leeds LS2 9JT, UK (fax +44 532 333167)Department of Cell Biology, AFRC institute of Plant Science Research, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors: P J
authors: PJ
authors: Linstead
authors: For correspondance: centre for Plant Biochemistry and Biotechnology, Unversity of Leeds, Leeds LS2 9JT, UK (fax +44 532 333167)Department of Cell Biology, AFRC institute of Plant Science Research, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors: J Peart C
authors: JPC
authors: Cooper
authors: For correspondance: centre for Plant Biochemistry and Biotechnology, Unversity of Leeds, Leeds LS2 9JT, UK (fax +44 532 333167)Department of Cell Biology, AFRC institute of Plant Science Research, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.
authors: K
authors: K
authors: Roberts
mesh_terms: D000911:Antibodies, Monoclonal; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D018552:Daucus carota / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000502:physiology; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D018519:Meristem / Q000276:immunology; D008970:Molecular Weight; D009088:Mucoproteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D019441:Plant Epidermis / Q000166:cytology; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology / Q000502:physiology; D018517:Plant Roots / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D052584:Xylem / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins