title: Tracing the endocytosis of claudin-5 in brain endothelial cells.
abstract: Claudin-5 is a transmembrane tight junction protein highly expressed in brain endothelial cells, the site of the blood-brain barrier. The properties of the brain endothelial tight junction complex are considered to be dependent on claudin-5 cell-cell interaction, putting this protein in a position to play a major role in the maintenance of brain endothelial barrier integrity. Thus, alterations in claudin-5 function can lead to "opening" of the paracellular route and increased brain endothelial barrier permeability. Recent work from the authors's laboratory has established that caveolae-dependent internalization/recycling of claudin-5 is a mechanism underlying transient increases in brain endothelial paracellular permeability in the presence of pro-inflammatory mediators. The biochemical and microscopic techniques presented here were used to investigate trafficking of claudin-5 during those changes in paracellular permeability.
authors: Svetlana M
authors: SM
authors: Stamatovic
authors: Richard F
authors: RF
authors: Keep
authors: Anuska V
authors: AV
authors: Andjelkovic
mesh_terms: D000818:Animals; D019921:Biotinylation; D015153:Blotting, Western / Q000379:methods; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D022461:Caveolins / Q000378:metabolism; D062507:Claudin-5; D004705:Endocytosis / Q000502:physiology*; D042783:Endothelial Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique / Q000379:methods; D049452:Green Fluorescent Proteins / Q000378:metabolism; D047468:Immunoprecipitation / Q000379:methods; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D059008:Time-Lapse Imaging / Q000379:methods; D022161:Transport Vesicles / Q000378:metabolism
chemical_list: D022461:Caveolins; D062507:Claudin-5; C491511:Cldn5 protein, mouse; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins