title: Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development.
abstract: A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623 bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4 kDa and theoretical pI of 8.45. Six cysteine residues and a potential calcium binding motif that are present in AdLys c-1 are highly conserved relative to those of c-type lysozymes found in other insects. RT-PCR analysis of the AdLys c-1 transcript revealed its presence at high levels in the salivary glands both in larval and adult stages and in the larval caecum. dsRNA mediated gene knockdown experiments were conducted to examine the potential role of this lysozyme during Plasmodium berghei infection. Silencing of AdLys c-1 resulted in a significant reduction in the number of oocysts as compared to control dsGFP injected mosquitoes.
authors: Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
authors: Parichat
authors: P
authors: Lapcharoen
authors: Narumon
authors: N
authors: Komalamisra
authors: Yupha
authors: Y
authors: Rongsriyam
authors: Voranuch
authors: V
authors: Wangsuphachart
authors: Paron
authors: P
authors: Dekumyoy
authors: Jetsumon
authors: J
authors: Prachumsri
authors: Mayur K
authors: MK
authors: Kajla
authors: Susan M
authors: SM
authors: Paskewitz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D017124:Conserved Sequence / Q000235:genetics; D003545:Cysteine / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D007814:Larva; D008288:Malaria / Q000469:parasitology / Q000635:transmission*; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000378:metabolism; D010962:Plasmodium berghei / Q000502:physiology; D012469:Salivary Glands / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D009113:Muramidase; D003545:Cysteine