title: [Expressions of aquaporins 1 and 3 in the mouse prostate and their significance].
abstract: OBJECTIVE
  To investigate the expressions of aquaporins (AQPs) in the mouse prostatic tissue and their significance, and to provide some evidence for a deeper insight into the physiological function and regulation of AQP expressions in normal and diseased prostatic tissues.
  
  
  METHODS
  The mRNA expressions of AQP0 - 4 in the mouse prostatic tissue were determined by RT-PCR, and the expressions and localizations of AQP1 and AQP3 proteins were characterized by Western blot and immunohistochemistry.
  
  
  RESULTS
  RT-PCR exhibited the mRNA expressions of AQP1 and AQP3, but not those of AQP0, AQP2 and AQP4 in the prostate tissue, while Western blot showed the expression of the AQP1 protein with the relative molecular mass (RMM) of 28 000 and those of the glycosylated and non-glycosylated AQP3 proteins with the RMM of 35 000 and 27 000, respectively. Immunohistochemistry indicated the strong expression of AQP1 in the cyst and plasma membrane of the secretary cells and that of AQP3 in the stroma cells of the prostate.
  
  
  CONCLUSIONS
  The AQP1 and AQP3 genes were expressed in the secretary epithelia of the mouse prostate tissue, which suggests that AQP1 and AQP3 may play an important role in the secretion of prostatic fluid.
authors: Department of Distinguished Professor Tang Ao-qing Laboratory, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China.
authors: Jian-Guo
authors: JG
authors: Zhu
authors: Mei
authors: M
authors: Wu
authors: Ya-Nan
authors: YN
authors: Liu
authors: Dan
authors: D
authors: Zhao
mesh_terms: D000818:Animals; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D051400:Aquaporin 3 / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011467:Prostate / Q000378:metabolism* / Q000473:pathology
chemical_list: C496021:Aqp1 protein, mouse; C496028:Aqp3 protein, mouse; D051398:Aquaporin 1; D051400:Aquaporin 3