title: Enhanced resistance of Saccharomyces cerevisiae to vanillin by expression of lacA from Trametes sp. AH28-2.
abstract: Saccharomyces cerevisiae is affected by the presence of certain phenolic compounds such as vanillin during fermentation of pretreated lignocellulosic hydrolysates. Since vanillin can be polymerized in the presence of laccase into compounds with lower toxicity, the laccase gene, lacA, from Trametes sp. AH28-2 was fused to the α-factor signal sequence and transferred into S. cerevisiae CEN.PK strains for secretory expression. Furthermore, the chaperone gene, KAR2, was overexpressed to promote the translocation of laccase. In the presence of 8 mmol/L vanillin, a shorter lag phase was observed in the lacA gene expressing strains. The vanillin-specific conversion rate of the lacA-expressing strain BSJX0A2 was 0.069 g g(-1)biomass h(-1), while it was 0.065 g g(-1)biomass h(-1) in the reference strain.
authors: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors: Lei
authors: L
authors: Ji
authors: Yu
authors: Y
authors: Shen
authors: Lili
authors: L
authors: Xu
authors: Bingyin
authors: B
authors: Peng
authors: Yazhong
authors: Y
authors: Xiao
authors: Xiaoming
authors: X
authors: Bao
mesh_terms: D001483:Base Sequence; D001547:Benzaldehydes / Q000494:pharmacology*; D018533:Biomass; D017931:DNA Primers; D042845:Laccase / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D055454:Trametes / Q000201:enzymology / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D017931:DNA Primers; C100058:vanillin; D042845:Laccase