title: Targeting genes in living mammals by RNA interference.
abstract: More than a decade has passed since the discovery of RNA interference (RNAi), an eukaryotic sequence-specific degradation of mRNA induced by complementary double-stranded RNA (dsRNA). RNAi became a common tool for controlled down-regulation of gene expression in cultured cells, as well as in various model organisms. This review summarizes RNAi-based tools for silencing genes in living mammals, which include: (i) transgenic RNAi strategies, where RNAi is triggered by a transgene transmitted through the germline and (ii) approaches, where an RNAi trigger is delivered into an adult animal.
authors: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska, Prague, Czech Republic.
authors: Katerina
authors: K
authors: Podolska
authors: Petr
authors: P
authors: Svoboda
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D055785:Gene Knockdown Techniques / Q000379:methods*; D015316:Genetic Therapy; D008322:Mammals / Q000235:genetics*; D034622:RNA Interference