title: Isoarnottinin 4'-glucoside, a glycosylated coumarin from Prangos uloptera, with biological activity.
abstract: Coumarins are a well-known group of natural products distributed in the plant kingdom especially in the family Apiaceae with various biological activities. Isoarnottinin 4'-glucoside is a simple glycosylated coumarin found previously in a few genera of Apiaceae, and its biological activities have not been previously described in details. In the present paper, the compound was isolated from Prangos uloptera (Apiaceae) leaves using HPLC techniques. Antimicrobial, phytotoxic and cytotoxic activities of the compound were evaluated by disk diffusion, lettuce assay and MTT method. Our results indicated that the compound has high antibacterial effect against Erwinia carotovora, a common plant pathogen with MIC value of 100 microg/mL. The compound also exhibited significant phytotoxic activity against lettuce and modest cytotoxic activity against HeLa cell line with IC50 of 0 .84 mg/mL. It could be concluded that isoamottinin 4'-glucoside may play phytoalexin or allelopathic role for plant and may be a candidate for an antibacterial agent or a bioherbicide.
authors: Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. razavi694@gmail.com
authors: Seyed Mehdi
authors: SM
authors: Razavi
authors: Gholamreza
authors: G
authors: Zarrini
authors: Farah Gholami
authors: FG
authors: Rad
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D019661:Apiaceae / Q000502:physiology*; D017209:Apoptosis / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism* / Q000627:therapeutic use; D004756:Enterobacteriaceae Infections / Q000188:drug therapy* / Q000503:physiopathology; D018525:Germination / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism / Q000627:therapeutic use; D006031:Glycosylation; D006367:HeLa Cells; D006540:Herbicides / Q000378:metabolism; D006801:Humans; D018545:Lactuca / Q000187:drug effects* / Q000382:microbiology; D016973:Pectobacterium carotovorum / Q000378:metabolism* / Q000472:pathogenicity; D010675:Pheromones / Q000378:metabolism; D010935:Plant Diseases / Q000517:prevention & control; D018515:Plant Leaves
chemical_list: D000900:Anti-Bacterial Agents; D003374:Coumarins; D005960:Glucosides; D006540:Herbicides; D010675:Pheromones; C560109:isoarnottinin 4'-glucoside