title: [Evaluation of three methods for in vitro detection of antifungal susceptibility of Candida species].
abstract: Reference methods E.Def 7.1 and M27-A3 detect in vitro resistance; however, they are expensive and very laborious. Thus, their actual use in hospital laboratories is limited. There are commercial techniques available, having easier accessibility and development, which would yield results comparable to those of the reference methods. The objectives of this study were: a) to compare the results of minimal inhibitory concentration of 82 Candida spp. clinic isolates according to reference method E.Def 7.1 and ATB® Fungus 3; b) to compare the results of fluconazole susceptibility testing by disk diffusion in agar with Neo-SensitabsTM tablets and Malbrán disks with those of the reference methods. Minimal inhibitory concentration for amphotericin B, 5-flucytosine, fluconazole and itraconazole was performed according to the E.Def 7.1 and the ATB Fungus 3 methods and diffusion was carried out with fluconazole disks and tablets. General concordance between the reference method and ATB Fungus 3 was 90.2 % and 96.3 and 92.7% for diffusion with disks and tablets. The ATB Fungus 3 method was effective to determine susceptibility against amphotericin B and 5-flucytosine; however, discrepancies were observed with azole drugs. Disk diffusion methods are useful to determine susceptibility to fluconazole; however, 3 very major, 1 major and 2 minor errors were observed with the tablets, whereas only 3 minor errors were observed with the disks.
authors: Sección Microbiología, Laboratorio Central, Hospital Alemán, Ciudad Autónoma de Buenos Aires, Argentina. ivanam27@gmail.com
authors: Ivana
authors: I
authors: Maldonado
authors: Liliana
authors: L
authors: Fernández Canigia
authors: Walter
authors: W
authors: Vivot
authors: Patricia
authors: P
authors: Domecq
authors: Graciela
authors: G
authors: Davel
authors: Susana
authors: S
authors: Córdoba
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D001118:Argentina; D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000382:microbiology; D052978:Disk Diffusion Antimicrobial Tests; D025141:Drug Resistance, Fungal; D026141:Drug Resistance, Multiple, Fungal; D005260:Female; D015725:Fluconazole / Q000494:pharmacology; D005437:Flucytosine / Q000494:pharmacology; D006801:Humans; D017964:Itraconazole / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D013045:Species Specificity; D013607:Tablets
chemical_list: D000935:Antifungal Agents; D013607:Tablets; D017964:Itraconazole; D000666:Amphotericin B; D015725:Fluconazole; D005437:Flucytosine