title: The detection of 3 leptin receptor isoforms in crucian carp gill and the influence of fasting and hypoxia on their expression.
abstract: To understand leptin signaling pathway in the crucian carp (Carassius carassius), we cloned 3 leptin receptor isoform complementary DNAs (ie, the long form [cclpr-L], the short form [cclpr-s1], and the secreted form [cclpr-s2]). Variant cclpr-L had a 3,255-bp open reading frame and a complete intracellular domain with box 1 and box 2 consensus sequences. By contrast, cclpr-s1 contained only 4 amino acids in its intracellular domain, without the "box 1" motif, which is conserved among membrane-bound leptin receptor short isoforms in mammals. Variant cclpr-s2 had no transmembrane domain, suggesting that it is a soluble form of the receptor, and alternative splicing of cclpr-s2 mRNA employs a different mechanism for the generation of soluble leptin receptor by intron retention. The fasting-treated fish showed significantly lower cclpr-L mRNA levels in gill tissue than the control group, whereas cclpr-s2 mRNA levels did not vary significantly among the groups. Treatment with hypoxia significantly increased mRNA levels of both cclpr-L and cclpr-s2 in gill tissue. To our knowledge, this is the first study of leptin receptor isoforms expression in teleosts.
authors: College of Chemistry and Life Science, Zhejiang Normal University, Jinhua Zhejiang, China. sky109@zjnu.edu.cn
authors: Y-B
authors: YB
authors: Cao
authors: J L
authors: JL
authors: Xue
authors: L-Y
authors: LY
authors: Wu
authors: W
authors: W
authors: Jiang
authors: P-N
authors: PN
authors: Hu
authors: J
authors: J
authors: Zhu
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D003001:Cloning, Molecular; D005880:Gills / Q000502:physiology*; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D054411:Receptors, Leptin / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D054411:Receptors, Leptin