title: Isolation and characterization of an immunoenhancing glucan from alkaline extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer.
abstract: A water-soluble glucan, isolated from the alkaline extract of the fruit bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to consist of (1→3,6)-linked, (1→3)-linked, (1→6)-linked, and terminal β-d-glucopyranosyl moieties in a relative proportion of approximately 1:2:1:1. This polysaccharide showed optimum activation of macrophages as well as splenocytes and thymocytes at 10μg/mL. Structural investigation was carried out using sugar analysis, methylation analysis, periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as:
authors: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India.
authors: Sanjoy Kumar
authors: SK
authors: Bhunia
authors: Biswajit
authors: B
authors: Dey
authors: Kankan K
authors: KK
authors: Maity
authors: Sukesh
authors: S
authors: Patra
authors: Soumitra
authors: S
authors: Mandal
authors: Swatilekha
authors: S
authors: Maiti
authors: Tapas K
authors: TK
authors: Maiti
authors: Samir R
authors: SR
authors: Sikdar
authors: Syed S
authors: SS
authors: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D020074:Lentinula / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D013154:Spleen / Q000166:cytology; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D005936:Glucans