title: Antioxidant and immunological activities of water-soluble polysaccharides from Aconitum kusnezoffii Reichb.
abstract: Aconitum kusnezoffii Reichb., one of the earliest recorded toxic species of genus Aconitum, has been used as traditional Chinese medicine and medicinal diet over the last 2500 years to treat heart failure congestion, neuralgia, rheumatism and gout, etc. In the present paper, four water-soluble polysaccharide fractions isolated from the tubers of A. kusnezoffii Reichb. were studied the antioxidant and immunological activities for the first time. In vitro antioxidant assays indicated that fraction WKCP-A had noticeable scavenging activities on DPPH radical, hydroxyl radical, superoxide anion, H(2)O(2) and self-oxidation of 1,2,3-phentriol, ferrous ion-chelating ability and reducing power. Moreover, the in vivo immunological assay exhibited that fractions WKCP-A and WKHP could more significantly enhance splenic lymphocyte proliferation and macrophage phagocytosis than other fractions. Therefore, the water-soluble polysaccharides from A. kusnezoffii Reichb., especially WKCP-A, have the potential to be explored as novel natural antioxidants and immunostimulating agents for using in functional foods or medicine.
authors: Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province, PR China.
authors: Tingting
authors: T
authors: Gao
authors: Shuai
authors: S
authors: Ma
authors: Jiayin
authors: J
authors: Song
authors: Hongtao
authors: H
authors: Bi
authors: Yanduo
authors: Y
authors: Tao
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D016166:Free Radical Scavengers / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007155:Immunologic Factors / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology*; D012685:Sepharose / Q000031:analogs & derivatives; D012995:Solubility / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000378:metabolism; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D007155:Immunologic Factors; D007502:Iron Chelating Agents; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; C047264:sepharose CL 6B; D012685:Sepharose; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl