title: Electrospun biomimic nanofibrous scaffolds of silk fibroin/hyaluronic acid for tissue engineering.
abstract: This study aimed to fabricate nanofibrous scaffolds which could biomimic the natural extracellular matrix from aqueous solutions of silk fibroin and hyaluronic acid blends (SF/HA) by means of electrospinning. Scanning electronic microscopy results indicated that electrospun SF/HA nanofibers were ribbon-shaped and their average width obviously decreased with the increase of HA content. However, there is no fiber observed when the volume of HA further increased to 50% of overall volume. After being treated with 75% ethanol vapor for 24 h, the fibers still remained their fibrous morphologies and presented good capability of water-resistance. Fourier transform infrared attenuated total reflectance spectroscopy, (13)C-CP-MAS nuclear magnetic resonance and differential scanning calorimetry results revealed that HA did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. Cell viability studies demonstrated that SF/HA nanofibrous scaffolds significantly promoted cell proliferation. Electrospun SF/HA nanofibers may provide an ideal biomimic tissue-engineering scaffold or vehicle for water-soluble drugs.
authors: a College of Materials and Textile Engineering, Jiaxing University , Zhejiang , 314001 , P. R. China.
authors: Kuihua
authors: K
authors: Zhang
authors: Linpeng
authors: L
authors: Fan
authors: Zhiyong
authors: Z
authors: Yan
authors: Qiaozhen
authors: Q
authors: Yu
authors: Xiumei
authors: X
authors: Mo
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D012831:Bombyx; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology; D000431:Ethanol / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D005740:Gases / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D007085:Ilium / Q000502:physiology; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D012996:Solutions; D013500:Surface Tension; D013552:Swine; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D005740:Gases; D012996:Solutions; D014867:Water; D000431:Ethanol; D006820:Hyaluronic Acid; D005349:Fibroins