title: Poly(m-phenylenediamine) nanospheres and nanorods: selective synthesis and their application for multiplex nucleic acid detection.
abstract: In this paper, we demonstrate for the first time that poly(m-phenylenediamine) (PMPD) nanospheres and nanorods can be selectively synthesized via chemical oxidation polymerization of m-phenylenediamine (MPD) monomers using ammonium persulfate (APS) as an oxidant at room temperature. It suggests that the pH value plays a critical role in controlling the the morphology of the nanostructures and fast polymerization rate favors the anisotropic growth of PMPD under homogeneous nucleation condition. We further demonstrate that such PMPD nanostructures can be used as an effective fluorescent sensing platform for multiplex nucleic acid detection. A detection limit as low as 50 pM and a high selectivity down to single-base mismatch could be achieved. The fluorescence quenching is attributed to photoinduced electron transfer from nitrogen atom in PMPD to excited fluorophore. Most importantly, the successful use of this sensing platform in human blood serum system is also demonstrated.
authors: State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, People's Republic of China.
authors: Yingwei
authors: Y
authors: Zhang
authors: Hailong
authors: H
authors: Li
authors: Yonglan
authors: Y
authors: Luo
authors: Xu
authors: X
authors: Shi
authors: Jingqi
authors: J
authors: Tian
authors: Xuping
authors: X
authors: Sun
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D057230:Limit of Detection; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D015341:Nucleic Acid Probes / Q000737:chemistry; D009696:Nucleic Acids / Q000032:analysis* / Q000097:blood; D010655:Phenylenediamines / Q000138:chemical synthesis* / Q000737:chemistry*; D013696:Temperature
chemical_list: D015341:Nucleic Acid Probes; D009696:Nucleic Acids; D010655:Phenylenediamines; C435915:poly(1,3-phenylenediamine)