title: Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch. & C.A.Mey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites.
abstract: The aim of this study is to evaluate the in vitro amoebicidal activity of the aqueous extracts of Pastinaca armenea and Inula oculus-christi from Turkey. In the presence of aqueous extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, I. oculus showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32.00 mg/ml extract solution in the media, no viable trophozoites were determined from the time of 24 h. Moreover, in the presence of 16.00 mg/ml I. oculus-christi extract, no viable trophozoites were detected in 72 h of the experiment. Effectiveness of I. oculus-christi extract was found moderate against the cysts. In the presence of 32.00 mg/ml extract, only 25.3% of the total cysts were killed effectively. In the case of P. armenea, 40.3% and 23.0% of the trophozoites and cysts were killed in the presence of 32.00 mg/ml extract at the end of the experimental process (72 h), respectively.
authors: Department of Parasitology, School of Medicine, Cumhuriyet University, 58140 Sivas, Turkey.
authors: Serpil
authors: S
authors: Degerli
authors: Seyda
authors: S
authors: Berk
authors: Erdogan
authors: E
authors: Malatyali
authors: Bektas
authors: B
authors: Tepe
mesh_terms: D048248:Acanthamoeba castellanii / Q000187:drug effects*; D000563:Amebicides / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D038143:Inula / Q000737:chemistry*; D028529:Pastinaca / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D033761:Spores, Protozoan / Q000187:drug effects; D013997:Time Factors; D053058:Trophozoites / Q000187:drug effects; D014421:Turkey
chemical_list: D000563:Amebicides; D010936:Plant Extracts