title: The interactions of nitric oxide and adenosine on penicillin-induced epileptiform activity in rats.
abstract: In this study, the influence of nitric oxide (NO) and adenosine systems on penicillin-induced epileptiform activity was examined in rats. NO donor, sodium nitroprusside (SNP, 50 micrograms per rat, i.c.v.) reduced the frequency but not the amplitude of epileptiform discharges. Non-selective NOS inhibitor, N-omega-nitro-L-arginine methyl ester (L-NAME, 100 micrograms per rat, i.c.v.) practically did not exert any effect on the spike frequency and amplitude. Adenosine (100 micrograms per rat, i.c.) reduced spike frequency but not the amplitude, whereas theophylline (100 micrograms per rat, i.c.v.) increased the mean spike frequency and amplitude of penicillin-induced epileptiform discharges. Co-injection of theophylline and L-NAME did not cause a further increase in the epileptiform activity compared with theophylline. When NO production was blocked with L-NAME, the inhibitory effects of adenosine were lost. The obtained results suggest that NO and adenosine may decrease penicillin-induced epileptiform activity in rats and that NO, at least in part, may mediate the anticonvulsant effect of adenosine.
authors: Department of Physiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey. mehmetyildirim@ktu.edu.tr.
authors: Mehmet
authors: M
authors: Yildirim
authors: Abdullah H
authors: AH
authors: Marangoz
authors: Mustafa
authors: M
authors: Ayyildiz
authors: Seyit
authors: S
authors: Ankarali
authors: Cafer
authors: C
authors: Marangoz
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000241:Adenosine / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D058256:Brain Waves / Q000187:drug effects*; D004195:Disease Models, Animal; D004347:Drug Interactions; D004569:Electroencephalography; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004827:Epilepsy / Q000139:chemically induced*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010406:Penicillins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013806:Theophylline / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D020030:Nitric Oxide Donors; D010406:Penicillins; D009599:Nitroprusside; D009569:Nitric Oxide; D013806:Theophylline; D000241:Adenosine; D019331:NG-Nitroarginine Methyl Ester