title: NELL-1 binds to APR3 affecting human osteoblast proliferation and differentiation.
abstract: Nel-like protein 1 (NELL-1) is an osteoinductive molecule associated with premature calvarial suture closure. Here we identified apoptosis related protein 3 (APR3), a membrane protein known as a proliferation suppressor, as a binding protein of NELL-1 by biopanning. NELL-1 and APR3 colocalized on the nuclear envelope of human osteoblasts. NELL-1 significantly inhibited proliferation of osteoblasts co-transfected with APR3 through further down-regulation of Cyclin D1. The co-expression of NELL-1 and APR3 enhanced Ocn and Bsp expression and mineralization. RNAi of APR3 significantly reduced the differentiation effect of NELL-1. These findings suggest that the effects of NELL-1 on osteoblastic differentiation and proliferation are partly through binding to APR3.
authors: Zhejiang California International NanoSystems Institute, Zhejiang University, Hangzhou, PR China.
authors: Xuan
authors: X
authors: Zou
authors: Jia
authors: J
authors: Shen
authors: Feng
authors: F
authors: Chen
authors: Kang
authors: K
authors: Ting
authors: Zhong
authors: Z
authors: Zheng
authors: Shen
authors: S
authors: Pang
authors: Janette N
authors: JN
authors: Zara
authors: John S
authors: JS
authors: Adams
authors: Chia
authors: C
authors: Soo
authors: Xinli
authors: X
authors: Zhang
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism; D006801:Humans; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism* / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D019151:Peptide Library; D011485:Protein Binding / Q000502:physiology
chemical_list: C000631012:ATRAID protein, human; D051017:Apoptosis Regulatory Proteins; D002135:Calcium-Binding Proteins; D026901:Membrane Transport Proteins; C104144:NELL1 protein, human; D009419:Nerve Tissue Proteins; D019151:Peptide Library; D019938:Cyclin D1