title: Transcriptional variations mediated by an alternative promoter of the FPR3 gene.
abstract: Formyl peptide receptor 3 (FPR3) is a potential player in innate immunity and appears with FPR2 as a FPR cluster during primate evolution. Comparative genome analyses indicate that a segmental duplication (SD) event upstream of the FPR3 gene after the divergence of New and Old World monkeys led to the emergence of an alternative promoter. In this study we combined computational and experimental approaches to identify a FPR3 gene that is controlled by an alternative promoter derived during a SD event. Its transcriptional activity was detected by quantitative reverse transcription polymerase chain reaction. Human alternative transcripts (FPR3-1 and FPR3-2) showed tissue-specific patterns with strong expressions in lung or uterus, while the FPR3-1 transcript of rhesus macaque is broadly expressed in various tissues. Overall, transcriptional variations of FPR3 occur by an alternative promoter during primate evolution.
authors: Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors: Hong-Seok
authors: HS
authors: Ha
authors: Jae-Won
authors: JW
authors: Huh
authors: Jeong-An
authors: JA
authors: Gim
authors: Kyudong
authors: K
authors: Han
authors: Heui-Soo
authors: HS
authors: Kim
mesh_terms: D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D019143:Evolution, Molecular; D020869:Gene Expression Profiling; D023061:Gene Order; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011323:Primates; D011401:Promoter Regions, Genetic; D044042:Receptors, Formyl Peptide / Q000235:genetics*; D056916:Segmental Duplications, Genomic; D014158:Transcription, Genetic
chemical_list: C469985:FPR3 protein, human; D044042:Receptors, Formyl Peptide