title: Expression of matrix metalloproteinase-9 and -12 in porcine lung infections.
abstract: Matrix metalloproteinases (MMPs) play a variety of roles during organogenesis, in the immune response and during acute and chronic diseases as well as in tissue remodelling. During the last decade, the pig has become used increasingly as a model for human diseases; however, studies on the expression of porcine MMPs are limited. In the present study species-specific antibodies were produced to investigate the expression of MMP-9 and MMP-12 immunohistochemically in lungs from pigs infected with Actinobacillus pleuropneumoniae, Pasteurella multocida and Staphylococcus aureus. The immunolabelling of lung tissues (one infected and one control pig representing each infection) was evaluated for cellular distribution and intensity, which was scored semiquantitatively. When compared with healthy, non-infected controls, the expression of both MMP-9 and MMP-12 was higher in infected lungs. The highest expressions were seen in the alveolar epithelium (MMP-9) and alveolar macrophages (MMP-12). These results are in accordance with studies of human lungs.
authors: Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark. cvb@life.ku.dk
authors: C S
authors: CS
authors: Bruun
authors: P S
authors: PS
authors: Leifsson
authors: L K
authors: LK
authors: Johansen
authors: H E
authors: HE
authors: Jensen
authors: J
authors: J
authors: Nielsen
authors: M
authors: M
authors: Fredholm
mesh_terms: D000189:Actinobacillus Infections / Q000201:enzymology* / Q000382:microbiology; D016977:Actinobacillus pleuropneumoniae; D000818:Animals; D008168:Lung / Q000201:enzymology / Q000382:microbiology; D008171:Lung Diseases / Q000201:enzymology* / Q000382:microbiology; D053508:Matrix Metalloproteinase 12 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010326:Pasteurella Infections / Q000201:enzymology* / Q000382:microbiology; D016979:Pasteurella multocida; D013203:Staphylococcal Infections / Q000201:enzymology* / Q000382:microbiology; D013211:Staphylococcus aureus; D013552:Swine
chemical_list: D020780:Matrix Metalloproteinase 9; D053508:Matrix Metalloproteinase 12