title: A set of novel microsatellite markers developed for the traditional Tibetan medicinal plant Halenia elliptica (Gentianaceae).
abstract: OBJECTIVE
  Microsatellite primers were developed in the traditional Tibetan medicinal plant Halenia elliptica D. Don to investigate its genetic diversity and population genetic structure.
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences Containing (FIASCO) repeats protocol, 24 primer sets were identified in two wild populations. Of these primers, 12 displayed polymorphisms and 12 were monomorphic. The number of alleles per locus ranged from 2 to 6, with a mean of 3.9. The expected (H(E)) and observed (H(O)) heterozygosities ranged from 0.191 to 0.784 and from 0.417 to 0.917, respectively. All these primers successfully amplified in two close relatives of H. elliptica, Swertia bimaculata (Siebold & Zucc.) Hook. f. & Thomson ex C. B. Clarke and S. tetraptera Maxim.
  
  
  CONCLUSIONS
  These markers will facilitate further studies on the population genetics of Halenia elliptica and its allied species.
authors: Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
authors: Zhi-Rong
authors: ZR
authors: Zhang
authors: Jing
authors: J
authors: Yang
authors: Yao
authors: Y
authors: Sun
authors: Tian-Gang
authors: TG
authors: Gao
authors: De-Zhu
authors: DZ
authors: Li
authors: Chun-Ying
authors: CY
authors: Xue
mesh_terms: D005821:Genetic Techniques; D005820:Genetic Testing; D029965:Gentianaceae / Q000235:genetics*; D038861:Medicine, Tibetan Traditional; D018895:Microsatellite Repeats / Q000235:genetics*; D010946:Plants, Medicinal / Q000235:genetics*