title: Glucose but not N-acetylglucosamine accelerates in vitro senescence of human peritoneal mesothelial cells.
abstract: BACKGROUND
  Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysis membrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG) has an identical effect.
  
  
  METHODS
  Replicative aging of MCs was studied during 10 passages performed every three days in cells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30 mmol/L. Changes in population doubling time and ß-galactosidase activity were used as an index of aging and compared with other cellular parameters.
  
  
  RESULTS
  Repeated passages of MC cause their aging, as reflected by prolongation of the population doubling time, increased ß-galactosidase activity, oxidative stress and release of cytokines. Healing of injured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC, whereas NAG does not cause this effect.
  
  
  CONCLUSIONS
  Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.
authors: Department of Pathophysiology, Poznan Medical School, Poznan, Poland.
authors: Marta
authors: M
authors: Ciszewicz
authors: George
authors: G
authors: Wu
authors: Paul
authors: P
authors: Tam
authors: Alicja
authors: A
authors: Polubinska
authors: Andrzej
authors: A
authors: Bre Borowicz
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D000704:Analysis of Variance; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D015314:Dialysis Solutions / Q000737:chemistry / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D009852:Omentum / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010530:Peritoneal Dialysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D015314:Dialysis Solutions; C508600:IL6 protein, human; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D006820:Hyaluronic Acid; D001616:beta-Galactosidase; D005947:Glucose; D000117:Acetylglucosamine