title: Suppression of regrowth of normal skin flora under chlorhexidine gluconate dressings applied to chlorhexidine gluconate-prepped skin.
abstract: BACKGROUND
  Catheter colonization and bloodstream infection during the first week after insertion of a central venous catheter have been shown to result from the patient's own skin flora.
  
  
  METHODS
  The backs of 32 healthy subjects were prepped with a 2% chlorhexidine gluconate (CHG)/70% isopropyl alcohol antiseptic. Three dressings, 2 of which contained CHG, were placed on the prepped skin in a randomized design. Samples of aerobic bacteria were collected using the cup scrub method. Skin under the dressings was sampled by quadrant on days 1, 4, and 7. Relative suppression of regrowth was compared using an adjusted paired t test.
  
  
  RESULTS
  Mean log counts were 3.2 log(10) colony-forming units (CFU)/cm(2) before antisepsis and 0.4 after antisepsis. Mean log counts obtained on days 1, 4, and 7 were 0.4, 0.3, and 0.5 log(10) CFU/cm(2) for the CHG gel; 0.4, 0.4, and 0.9 log(10) CFU/cm(2) for the CHG disk; and 0.9, 1.2, and 1.5 log(10) CFU/cm(2) for the Control, respectively.
  
  
  CONCLUSIONS
  Skin flora was not completely eradicated during antisepsis, and bacterial regrowth occurred postantisepsis. The use of CHG dressings helped sustain a reduced bacterial count on the skin. The continuously releasing CHG gel maintained suppression to a greater extent than the CHG disk at 7 days (P = .01).
authors: MICROBIOTEST, Division of Microbac Laboratories, Inc, Sterling, VA, USA.
authors: Muhammad H
authors: MH
authors: Bashir
authors: Linda K M
authors: LK
authors: Olson
authors: Shelley-Ann
authors: SA
authors: Walters
mesh_terms: D019840:2-Propanol / Q000008:administration & dosage; D000328:Adult; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage*; D001420:Bacteria, Aerobic / Q000187:drug effects* / Q000302:isolation & purification*; D001458:Bandages / Q000382:microbiology*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D015169:Colony Count, Microbial; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000382:microbiology*; D013997:Time Factors
chemical_list: D000891:Anti-Infective Agents, Local; C010882:chlorhexidine gluconate; D019840:2-Propanol; D002710:Chlorhexidine