title: Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles.
abstract: In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO(2)-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO(2)-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.
authors: Key Education Ministry Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China.
authors: Meihe
authors: M
authors: Zhang
authors: Ruo
authors: R
authors: Yuan
authors: Yaqin
authors: Y
authors: Chai
authors: Wenjuan
authors: W
authors: Li
authors: Huaan
authors: H
authors: Zhong
authors: Cun
authors: C
authors: Wang
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D005947:Glucose / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C009495:titanium dioxide; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D006046:Gold; D048271:Chitosan; D014025:Titanium; D005947:Glucose