title: Current trend of pneumococcal serotypes distribution and antibiotic susceptibility pattern in Malaysian hospitals.
abstract: From January 2008 to December 2009, 433 Streptococcus pneumoniae strains were examined to determine the serotype distribution and susceptibility to selected antibiotics. About 50% of them were invasive isolates. The strains were isolated from patients of all age groups and 33.55% were isolated from children below 5 years. The majority was isolated from blood (48.53%) and other sterile specimens (6.30%). Community acquired pneumonia (41.70%) is the most common diagnosis followed by sepsis (9.54%). Serotyping was done using Pneumotest Plus-Kit and antibiotic susceptibility pattern was determined by modified Kirby-Bauer disk diffusion method and measurement of minimum inhibitory concentration (MIC) using E-test strip. Ten most common serotypes were 19F (15.02%), 6B (10.62%), 19A (6.93%), 14 (6.70%), 1 (5.08%), 6A (5.08%), 23F (4.85%), 18C (3.93%), 3 (2.08%) and 5 (1.85%). Penicillin MIC ranged between ≤ 0.012-4 μg/ml with MIC₉₀ of 1 μg/ml. Penicillin resistant rate is 31.78%. The majority of penicillin less-susceptible strains belonged to serotype 19F followed by 19A and 6B. Based on the serotypes distribution 22 (44.00%), 28 (56.00%) and 39 (78.00%) of the invasive isolates from children ≤ 2 years were belonged to serotypes included in the PCV7, PCV10 and PCV13, respectively.
authors: Specialised Diagnostic Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. rohani@imr.gov.my
authors: Rohani M D
authors: RM
authors: Yasin
authors: Norni Mohd
authors: NM
authors: Zin
authors: Azura
authors: A
authors: Hussin
authors: Salbiah Haji
authors: SH
authors: Nawi
authors: Suhailah M D
authors: SM
authors: Hanapiah
authors: Zubaidah Abdul
authors: ZA
authors: Wahab
authors: Ganeswarie
authors: G
authors: Raj
authors: Norazita
authors: N
authors: Shafie
authors: Ng Pey
authors: NP
authors: Peng
authors: Keah K
authors: KK
authors: Chu
authors: Muhhamad Nazri
authors: MN
authors: Aziz
authors: Nurahan
authors: N
authors: Maning
authors: Jamilah Said
authors: JS
authors: Mohamad
authors: Adom
authors: A
authors: Benjamin
authors: Mohd Azmi Bin Mohd
authors: MA
authors: Salleh
authors: Sabarina Sabihah
authors: SS
authors: Zahari
authors: Alex
authors: A
authors: Francis
authors: Norazah
authors: N
authors: Ahmad
authors: Rina
authors: R
authors: Karunakaran
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000382:microbiology; D052978:Disk Diffusion Antimicrobial Tests; D024881:Drug Resistance, Bacterial; D005260:Female; D006801:Humans; D007223:Infant; D008296:Malaysia; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011008:Pneumococcal Infections; D011014:Pneumonia / Q000382:microbiology; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents