title: Holo-Ni2+ Helicobacter pylori NikR contains four square-planar nickel-binding sites at physiological pH.
abstract: The crystal structure of Helicobacter pylori holo-NikR, a Ni(2+)-dependent transcription factor, determined at pH 7.3, shows four square-planar nickel-binding sites, involving one cysteinate and three histidine ligands. This observation reconciles previous inconsistencies among calorimetric data, structural information at non-physiological pH, and computational studies.
authors: Faculty of Science and Technology, Free University of Bolzano, 39100, Bolzano, Italy. stefano.benini@unibz.it
authors: Stefano
authors: S
authors: Benini
authors: Michele
authors: M
authors: Cianci
authors: Stefano
authors: S
authors: Ciurli
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D016480:Helicobacter pylori; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009532:Nickel / Q000737:chemistry*; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D009532:Nickel