title: Sugar-binding properties of the two lectin domains of LEC-1 with respect to the Galβ1-4Fuc disaccharide unit present in protostomia glycoconjugates.
abstract: Galβ1-4Fuc disaccharide unit was recently reported to be the endogenous structure recognized by the galectin LEC-6 isolated from the nematode Caenorhabditis elegans. LEC-1, which is another major galectin from this organism, is a tandem repeat-type galectin that contains two carbohydrate recognition domains, the N-terminal lectin domain (LEC-1Nh) and the C-terminal lectin domain (LEC-1Ch), and was also found to have an affinity for the Galβ1-4Fuc disaccharide unit. In the present study, we compared the binding strengths of LEC-1, LEC-1Nh, and LEC-1Ch to Galβ1-4Fuc, Galβ1-3Fuc, and Galβ1-4GlcNAc units as well as to LEC-6-ligand N-glycans by using frontal affinity chromatography (FAC) analysis. The two lectin domains of LEC-1 exhibited the highest affinity for Galβ1-4Fuc, though sugar-binding properties differed somewhat between LEC-1Nh and LEC-1Ch. Furthermore, these two domains had significantly lower affinities for the LEC-6-binding glycans. These results suggest that the endogenous recognition unit of LEC-1 is likely to be Galβ1-4Fuc, and that the endogenous ligands for LEC-1 are different from those for LEC-6.
authors: Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University, 1–1 Keyakidai, Sakado, Saitama 350–0295, Japan.
authors: Tomoharu
authors: T
authors: Takeuchi
authors: Ken-Ichi
authors: K
authors: Sugiura
authors: Kazusa
authors: K
authors: Nishiyama
authors: Hideyo
authors: H
authors: Takahashi
authors: Hideaki
authors: H
authors: Natsugari
authors: Yoichiro
authors: Y
authors: Arata
authors: Ken-Ichi
authors: K
authors: Kasai
mesh_terms: D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism*; D006001:Glycoconjugates / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D006001:Glycoconjugates; D037102:Lectins