title: Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.
abstract: The extra-cellular domain of the influenza virus matrix protein 2 (M2e) is highly conserved between influenza A virus strains compared to hemagglutinin and neuraminidase, and has long been viewed as a potential and universal vaccine target. M2e induces no or only weak and transient immune responses following infection, making it difficult to detect M2e-specific antibodies producing B-cells in human peripheral blood lymphocytes. Recently, using a single-cell manipulation method, immunospot array assay on a chip (ISAAC), we obtained an M2e-specific human antibody (Ab1-10) from the peripheral blood of a healthy volunteer. In this report, we have demonstrate that Ab1-10 reacted not only to seasonal influenza A viruses, but also to pandemic (H1N1) 2009 virus (2009 H1N1) and highly pathogenic avian influenza A virus, and that the antibody-bound M2e of 2009 H1N1 inactivated the virus with high affinity (∼10(-10)M). More importantly, it inhibited 2009 H1N1 viral propagation in vitro. These results suggest that Ab1-10 might be a potential candidate for antibody therapeutics for a wide range of influenza A viruses.
authors: Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan. tox@med.u-toyama.ac.jp
authors: Tatsuhiko
authors: T
authors: Ozawa
authors: Aishun
authors: A
authors: Jin
authors: Kazuto
authors: K
authors: Tajiri
authors: Masaya
authors: M
authors: Takemoto
authors: Tomoko
authors: T
authors: Okuda
authors: Kimiyasu
authors: K
authors: Shiraki
authors: Hiroyuki
authors: H
authors: Kishi
authors: Atsushi
authors: A
authors: Muraguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D001717:Birds; D058501:Enzyme-Linked Immunospot Assay; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000254:growth & development; D053124:Influenza A Virus, H5N1 Subtype / Q000187:drug effects* / Q000254:growth & development; D007252:Influenza Vaccines / Q000096:biosynthesis* / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D056656:Lab-On-A-Chip Devices; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D059010:Single-Cell Analysis; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014763:Viral Matrix Proteins