title: Spatial filter based 3D resolution improvement and polarization properties of multiphoton multiple-excitation-spot-optical microscopy.
abstract: Three-dimensional (3D) resolution improvement in multi-photon multiple-excitation-spot-optical microscopy is proposed. Specially designed spatial filter is employed for improving the overall 3D resolution of the imaging system. An improvement up to a factor of 14.5 and sub-femto liter volume excitation is achieved. The system shows substantial sidelobe reduction (<4%) due to the non-linear intensity dependence of multiphoton process. Polarization effect on x-oriented and freely rotating dipoles shows dramatic change in the field distribution at the focal-plane. The resulting point-spread function has the ability to produce several strongly localized polarization dependent field patterns which may find applications in optical engineering and bioimaging.
authors: Department of Instrumentation and Applied Physics, Indian Institute of Science, Bangalore 560012, India.
authors: Shilpa
authors: S
authors: Dilipkumar
authors: Alberto
authors: A
authors: Diaspro
authors: Partha Pratim
authors: PP
authors: Mondal
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D017785:Photons