title: Total chemical synthesis of biologically active vascular endothelial growth factor.
abstract: Efficient access: the 204-residue covalent-dimer vascular endothelial growth factor with full mitogenic activity was prepared from three unprotected peptide segments by one-pot native chemical ligations. The covalent structure of the synthetic VEGF was confirmed by precise mass measurement, and the three-dimensional structure of the synthetic protein was determined by high-resolution X-ray crystallography.
authors: Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, IL 60637, USA.
authors: Kalyaneswar
authors: K
authors: Mandal
authors: Stephen B H
authors: SB
authors: Kent
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D019281:Dimerization; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D042461:Vascular Endothelial Growth Factor A / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides; D042461:Vascular Endothelial Growth Factor A