title: Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry.
abstract: Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococcus aureus. The directionally released antibiotic alters S. aureus virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.
authors: Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA.
authors: David J
authors: DJ
authors: Gonzalez
authors: Center for Marine Biotechnology and Biomedicine, University of California at San Diego, La Jolla, CA 92093, USA.|Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Nina M
authors: NM
authors: Haste
authors: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Andrew
authors: A
authors: Hollands
authors: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Tinya C
authors: TC
authors: Fleming
authors: Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Matthew
authors: M
authors: Hamby
authors: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Kit
authors: K
authors: Pogliano
authors: Department of Pediatrics, University of California at San Diego, La Jolla, CA 92093, USA.|Center for Marine Biotechnology and Biomedicine, University of California at San Diego, La Jolla, CA 92093, USA.|Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Victor
authors: V
authors: Nizet
authors: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, USA.|Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, USA.|Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093, USA.
authors: Pieter C
authors: PC
authors: Dorrestein
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000302:isolation & purification / Q000378:metabolism*; D018920:Coculture Techniques; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006801:Humans; D055666:Lipopeptides / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D055666:Lipopeptides; D009842:Oligopeptides; D010456:Peptides, Cyclic; C049901:plipastatin; C009365:surfactin peptide