title: [Production and characterization of the monoclonal antibodies against nonstructural 1 protein of DENV-4].
abstract: OBJECTIVE
  To produce monoclonal antibodies (mAbs) against nonstructural 1 protein (NS1) of DENV-4 and characterization.
  
  
  METHODS
  BALB/c mice were immunized with recombinant DENV4-NS1 protein and inactive DENV-4. Splenocytes of immunized mice were fused with myeloma cells (NS-1) to produce hybridoma cell lines, secreting anti-DENV4-NS1 protein mAbs. ELISA was used to identify the mAbs against NS1of DENV-4. Immnofluorescence assay (IFA) and Western blot analysis were applied to identify the specificity of mAbs.
  
  
  RESULTS
  fifteen mAbs recognizing two different antigenic epitopes on NS1of DENV-4 were obtained. These mAbs had characteristics of specific binding to recombinant NS1 of DENV-4. The IFA showed that all of the mAbs strongly reacted to DENV-4, while had no cross-reactivity to the other three serotypes of dengue virus.
  
  
  CONCLUSIONS
  mAbs against NS1 of DENV-4 with high specificity has been successfully established. These results lay the foundation value for study on the structural and functional of DENV4-NS1 protein and early diagnosis of DENV-4 infection.
authors: Zhujiang Hospital, Southern Medical University, Guangzhou, China. dingxixia@126.com
authors: Xi-xia
authors: XX
authors: Ding
authors: Biao
authors: B
authors: DI
authors: Xiao-yan
authors: XY
authors: Che
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D003716:Dengue Virus; D000939:Epitopes / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D017361:Viral Nonstructural Proteins