title: Development and evaluation of nanosized niosomal dispersion for oral delivery of Ganciclovir.
abstract: Encapsulation of Ganciclovir in lipophilic vesicular structure may be expected to enhance the oral absorption and prolong the existence of the drug in the systemic circulation. So the purpose of the present study was to improve the oral bioavailability of Ganciclovir by preparing nanosized niosomal dispersion. Niosomes were prepared from Span40, Span60, and Cholesterol in the molar ratio of 1:1, 2:1, 3:1, and 3:2 using reverse evaporation method. The developed niosomal dispersions were characterized for entrapment efficiency, size, shape, in vitro drug release, release kinetic study, and in vivo performance. Optimized formulation (NG8; Span60:Cholesterol 3:2 molar ratio) has shown a significantly high encapsulation of Ganciclovir (89±2.13%) with vesicle size of 144±3.47 nm (polydispersity index [PDI]=0.08). The in vitro release study signifies sustained release profile of niosomal dispersions. Release profile of prepared formulations have shown that more than 85.2±0.015% drug was released in 24 h with zero-order release kinetics. The results obtained also revealed that the types of surfactant and Cholesterol content ratio altered the entrapment efficiency, size, and drug release rate from niosomes. In vivo study on rats reveals five-time increment in bioavailability of Ganciclovir after oral administration of optimized formulation (NG8) as compared with tablet. The effective drug concentration (>0.69 µg/mL in plasma) was also maintained for at least 8 h on administration of the niosomal formulation. In conclusion, niosomes can be proposed as a potential oral delivery system for the effective delivery of Ganciclovir.
authors: Nanoformulation Research Lab, Faculty of Pharmacy, Hamdard University, New Delhi, India.
authors: Sohail
authors: S
authors: Akhter
authors: Shalini
authors: S
authors: Kushwaha
authors: Musarrat H
authors: MH
authors: Warsi
authors: Mohammed
authors: M
authors: Anwar
authors: Mohammad Zaki
authors: MZ
authors: Ahmad
authors: Iqbal
authors: I
authors: Ahmad
authors: Sushma
authors: S
authors: Talegaonkar
authors: Zeenat I
authors: ZI
authors: Khan
authors: Roop K
authors: RK
authors: Khar
authors: Farhan J
authors: FJ
authors: Ahmad
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001682:Biological Availability; D002626:Chemistry, Pharmaceutical; D002784:Cholesterol / Q000737:chemistry; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D015774:Ganciclovir / Q000008:administration & dosage* / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010599:Pharmacokinetics; D051381:Rats; D013501:Surface-Active Agents / Q000737:chemistry; D044366:Transition Temperature
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008081:Liposomes; D013501:Surface-Active Agents; D002784:Cholesterol; D015774:Ganciclovir