title: Correlation of the Ga-68-bombesin analog Ga-68-BZH3 with receptors expression in gliomas as measured by quantitative dynamic positron emission tomography (dPET) and gene arrays.
abstract: OBJECTIVE
  The kinetics of Ga-68-BZH3, a Ga-68-bombesin analog, was compared to molecular biological data obtained from gene arrays in seven patients with a recurrent glioma. The primary aim of this study was the correlation of receptor expression and tracer kinetics.
  
  
  METHODS
  Dynamic positron emission tomography studies were performed and the data were analyzed by a volume-of-interest technique using a two-tissue compartment model as well as a non-compartment model. Gene array data were obtained from gene array analysis of tumor tissue samples.
  
  
  RESULTS
  The correlation analysis revealed a significant nonlinear correlation of r = 0.89 (p < 0.03) for k1 and BB(2) (gastrin-releasing peptide receptor). BB(1) and BB(3) were not significantly correlated with k1. vb and k3 were not significantly correlated with the expression data of the receptors on the p < 0.05 level.
  
  
  CONCLUSIONS
  The parameter k1 is correlated with the expression of BB(2) based on gene array data. The quantitative analysis of the Ga-68-BZH3 kinetics can be used to predict the receptor expression of BB(2) in gliomas based on k1 of the compartment analysis. However, this study is limited to the expression data on the mRNA level and further studies are needed to assess the correlation of gene expression on the protein level.
authors: Medical PET Group-Biological Imaging [E060-1], CCU Nuclear Medicine, German Cancer Research Center, 69120, Heidelberg, Germany. lgs@ads-lgs.de
authors: Ludwig G
authors: LG
authors: Strauss
authors: Dirk
authors: D
authors: Koczan
authors: Marcel
authors: M
authors: Seiz
authors: Jochen
authors: J
authors: Tuettenberg
authors: Kirsten
authors: K
authors: Schmieder
authors: Leyun
authors: L
authors: Pan
authors: Caixia
authors: C
authors: Cheng
authors: Antonia
authors: A
authors: Dimitrakopoulou-Strauss
mesh_terms: D001839:Bombesin / Q000031:analogs & derivatives* / Q000378:metabolism; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D005710:Gallium Radioisotopes / Q000378:metabolism*; D020869:Gene Expression Profiling; D005910:Glioma / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017711:Nonlinear Dynamics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods*; D011849:Radioactive Tracers; D018004:Receptors, Bombesin / Q000235:genetics* / Q000378:metabolism; D046888:Tissue Array Analysis
chemical_list: D005710:Gallium Radioisotopes; D011849:Radioactive Tracers; D018004:Receptors, Bombesin; D001839:Bombesin