title: Ultrasound-mediated interferon β gene transfection inhibits growth of malignant melanoma.
abstract: We investigated the effects of ultrasound-mediated transfection (sonotransfection) of interferon β (IFN-β) gene on melanoma (C32) both in vitro and in vivo. C32 cells were sonotransfected with IFN-β in vitro. Subcutaneous C32 tumors in mice were sonicated weekly immediately after intra-tumor injection with IFN-β genes mixed with microbubbles. Successful sonotransfection with IFN-β gene in vitro was confirmed by ELISA, which resulted in C32 growth inhibition. In vivo, the growth ratio of tumors transfected with IFN-β gene was significantly lower than the other experimental groups. These results may lead to a new method of treatment against melanoma and other hard-to-treat cancers.
authors: Department of Dermatology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka City 814-0180, Japan.
authors: Kazuki
authors: K
authors: Yamaguchi
authors: Loreto B
authors: LB
authors: Feril
authors: Katsuro
authors: K
authors: Tachibana
authors: Akira
authors: A
authors: Takahashi
authors: Miki
authors: M
authors: Matsuo
authors: Hitomi
authors: H
authors: Endo
authors: Yoshimi
authors: Y
authors: Harada
authors: Juichiro
authors: J
authors: Nakayama
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics* / Q000378:metabolism; D008545:Melanoma / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012878:Skin Neoplasms / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics
chemical_list: D016899:Interferon-beta