title: Electrochemical and spectroscopic study on the interaction between isoprenaline and DNA using multivariate curve resolution-alternating least squares.
abstract: Interaction of isoprenaline (ISO) with calf-thymus DNA was studied by spectroscopic and electrochemical methods. The behavior of ISO was investigated at a glassy carbon electrode (GCE) by cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV); ISO was oxidized and an irreversible oxidation peak was observed. The binding constant K and the stoichiometric coefficient m of ISO with DNA were evaluated. Also, with the addition of DNA, hyperchromicity of the UV-vis absorption spectra of ISO was noted, while the fluorescence intensity decreased significantly. Multivariate curve resolution-alternating least squares (MCR-ALS) chemometrics method was applied to resolve the combined spectroscopic data matrix, which was obtained by the UV-vis and fluorescence methods. Pure spectra of ISO, DNA and ISO-DNA complex, and their concentration profiles were then successfully obtained. The results indicated that the ISO molecule intercalated into the base-pairs of DNA, and the complex of ISO-DNA was formed.
authors: The State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China. ynni@ncu.edu.cn
authors: Yongnian
authors: Y
authors: Ni
authors: Min
authors: M
authors: Wei
authors: Serge
authors: S
authors: Kokot
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D055664:Electrochemical Techniques; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007545:Isoproterenol / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D010084:Oxidation-Reduction; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D002244:Carbon; D004247:DNA; C427203:calf thymus DNA; D007545:Isoproterenol