title: Synthesis, characterization, nucleic acid binding, and cytotoxic activity of a Ru(II) polypyridyl complex.
abstract: The binding properties of [Ru(bpy)(2)(H(2)IIP)](2+) (1) {bpy=2,2'-bipyridine, H(2)IIP=2-(indole-3-yl)-imidazolo[4,5-f][1,10]phenanthroline} with calf thymus DNA (CT-DNA) and yeast tRNA have been investigated comparatively by different spectroscopic and viscosity measurements. The results suggest that the affinity of complex 1 binding with yeast tRNA is stronger than that of complex 1 binding with CT-DNA, and complex 1 is a better enantioselective binder to yeast tRNA than to CT-DNA. The toxicity of complex 1 was concentration dependent, and HL-60 cells are more sensitive to complex 1 than Hep-G2 cells; complex 1 could induce Hep-G2 cell apoptosis.
authors: Key Lab of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan, P.R. China.
authors: Jin
authors: J
authors: Liu
authors: Li-Feng
authors: LF
authors: Tan
authors: Lian-He
authors: LH
authors: Jin
authors: Fang
authors: F
authors: Luan
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D018922:HL-60 Cells; D056945:Hep G2 Cells; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008024:Ligands; D046911:Macromolecular Substances / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008954:Models, Biological; D009696:Nucleic Acids / Q000378:metabolism*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D003603:Cytotoxins; D007364:Intercalating Agents; D008024:Ligands; D046911:Macromolecular Substances; D009696:Nucleic Acids; D010618:Phenanthrolines; D011108:Polymers; D015082:2,2'-Dipyridyl; D012428:Ruthenium