title: The effect of butyric acid on normal tendons: a potential stimulus for extracellular matrix expression.
abstract: We propose comparing angiogenic effects of butyric acid (BA)-impregnated suture vs control suture on an aged tendon model. Twenty-four 3-year-old rabbits underwent bilateral Achilles tendon exposure. BA-impregnated orthopedic suture was sutured into one side, and a control orthopedic suture into the contralateral side similarly. The rabbits were sacrificed at 7, 30, and 45 days and the tendons harvested for gross, histologic, and biochemical study. Histologically, there was increased vascularity/cell migration at all time points in the BA-treated tendons; proteoglycan expression (ie, safranin O staining) increased at 30 and 45 days. DNA concentration was significantly (P = .05) higher in the BA-treated tendon group relative to the control group at 7 days but was unchanged at 30 and 45 days. Similarly, messenger RNA (mRNA) expression of vascular endothelial growth factor (VEGF) was significantly (P = .05) higher in the BA-treated tendon at 7 days. A trend (P = .12) for higher expression in the BA group also was found at 30 days.
authors: San Diego Arthroscopy and Sports Medicine Center, San Diego, California, USA.
authors: Sean C
authors: SC
authors: Tracy
authors: James P
authors: JP
authors: Tasto
authors: Yasushi
authors: Y
authors: Oshima
authors: Ryo
authors: R
authors: Murata
authors: Jason
authors: J
authors: Garcia
authors: David
authors: D
authors: Amiel
mesh_terms: D000125:Achilles Tendon / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D020148:Butyric Acid / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006633:Histamine Antagonists / Q000494:pharmacology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D006633:Histamine Antagonists; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D020148:Butyric Acid; D004247:DNA