title: MicroRNA 132 alters sleep and varies with time in brain.
abstract: MicroRNA (miRNA) levels in brain are altered by sleep deprivation; however, the direct effects of any miRNA on sleep have not heretofore been described. We report herein that intracerebroventricular application of a miRNA-132 mimetic (preMIR-132) decreased duration of non-rapid-eye-movement sleep (NREMS) while simultaneously increasing duration of rapid eye movement sleep (REMS) during the light phase. Further, preMIR-132 decreased electroencephalographic (EEG) slow-wave activity (SWA) during NREMS, an index of sleep intensity. In separate experiments unilateral supracortical application of preMIR-132 ipsilaterally decreased EEG SWA during NREMS but did not alter global sleep duration. In addition, after ventricular or supracortical injections of preMIR-132, the mimetic-induced effects were state specific, occurring only during NREMS. After local supracortical injections of the mimetic, cortical miRNA-132 levels were higher at the time sleep-related EEG effects were manifest. We also report that spontaneous cortical levels of miRNA-132 were lower at the end of the sleep-dominant light period compared with at the end of the dark period in rats. Results suggest that miRNAs play a regulatory role in sleep and provide a new tool for investigating sleep regulation.
authors: Washington State University-Spokane, Health Sciences Bldg. 280E, 412 E Spokane Falls Blvd., Spokane, WA 99202, USA. cjdavis@wsu.edu
authors: Christopher J
authors: CJ
authors: Davis
authors: James M
authors: JM
authors: Clinton
authors: Ping
authors: P
authors: Taishi
authors: Stewart G
authors: SG
authors: Bohnet
authors: Kimberly A
authors: KA
authors: Honn
authors: James M
authors: JM
authors: Krueger
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002940:Circadian Rhythm; D004569:Electroencephalography; D007276:Injections, Intraventricular; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D018716:Molecular Mimicry; D009841:Oligonucleotides / Q000008:administration & dosage / Q000378:metabolism; D017440:Photoperiod; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep; D012895:Sleep, REM; D013997:Time Factors
chemical_list: C548821:MIRN132 microRNA, rat; D035683:MicroRNAs; D009841:Oligonucleotides