title: 3H-L-leucine transport by the promiscuous crustacean dipeptide-like cotransporter.
abstract: The crustacean intestine and hepatopancreas display a variety of solute transport mechanisms for transmembrane transfer of dietary contents from lumen to epithelial cytosol. An in vitro intestinal perfusion apparatus was used to characterize mucosal to serosoal (MS) and serosal to mucosal (SM) Zn(2+) -dependent (3)H-L-leucine transport by the intestine of the American lobster, Homarus americanus. Transmural 20 µM MS (3)H-L-leucine fluxes across lobster intestine were a hyperbolic function of luminal zinc concentration (1-50 µM) following Michaelis-Menten kinetics (K(m) = 2.67 ± 0.74 µM; J(max) = 19.56 ± 2.22 pmol/cm(2) ×min). Transmural 20 µM SM (3)H-L-leucine fluxes were not affected by serosal zinc, resulting in a highly significant stimulation of net amino acid transfer to the blood by luminal metal. MS fluxes of 20 µM (3)H-L-leucine were also hyperbolic functions of luminal [Cu(2+)], [Mn(2+)], [Na(+)], and [H(+)]. MS flux of (3)H-L-leucine was a sigmoidal function of luminal [L-leucine] and was stimulated by the addition of 20 µM luminal zinc at both pH 7.0 and 5.5. A greater enhanced amino acid transport occurred at the lower pH 5.5. MS flux of 20 µM (3)H-L-leucine in the presence of 20 µM zinc was significantly inhibited by addition of 100 µM luminal glycylsarcosine, and MS flux of 20 µM (3)H-glycylsarcosine was inhibited by 100 µM L-leucine in the presence of 20 µM zinc. Results suggest that (3)H-L-leucine and metals form a complex (e.g., Leu-Zn-Leu] that may functionally mimic dipeptides and use a dipeptide-like transporter during MS fluxes as suggested for fish and mammals.
authors: Department of Biology, University of North Florida, Jacksonville, Florida 32224, USA.
authors: I
authors: I
authors: Obi
authors: A L
authors: AL
authors: Wells
authors: P
authors: P
authors: Ortega
authors: D
authors: D
authors: Patel
authors: L
authors: L
authors: Farah
authors: F P
authors: FP
authors: Zanotto
authors: G A
authors: GA
authors: Ahearn
mesh_terms: D026905:Amino Acid Transport Systems / Q000378:metabolism*; D000818:Animals; D002412:Cations / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004151:Dipeptides / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000378:metabolism; D008121:Nephropidae / Q000378:metabolism*; D014316:Tritium; D015032:Zinc / Q000378:metabolism*
chemical_list: D026905:Amino Acid Transport Systems; D002412:Cations; D004151:Dipeptides; D014316:Tritium; D007930:Leucine; D015032:Zinc