title: Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning.
abstract: Cell patterning methods enable researchers to control specific homotypic and heterotypic contact-mediated cell-cell and cell-ECM interactions and to impose defined cell and tissue geometries. To micropattern individual cells to specific points on a substrate with high spatial resolution, we have developed a cell deposition microscope based on the laser-guidance technique. We discuss the theory of optical forces for generating laser guidance and the optimization of the optical configuration (NA ≈ 0.1) to manipulate cells with high speed in three dimensions. Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing. Using this cell deposition microscope, rat mesenchymal stem cells from bone marrow were micropatterned with cardiomyocytes into a substrate microfabricated with polydimethylsiloxane on a 22 mm × 22 mm coverglass to form a single-cell coculturing microenvironment, and their electrophysiological property changes were investigated during the coculturing days.
authors: Department of Bioengineering, COMSET, Clemson University, Clemson, SC 29634, USA.
authors: Zhen
authors: Z
authors: Ma
authors: Russell K
authors: RK
authors: Pirlo
authors: Qin
authors: Q
authors: Wan
authors: Julie X
authors: JX
authors: Yun
authors: Xiaocong
authors: X
authors: Yuan
authors: Peng
authors: P
authors: Xiang
authors: Thomas K
authors: TK
authors: Borg
authors: Bruce Z
authors: BZ
authors: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004566:Electrodes; D007834:Lasers; D059630:Mesenchymal Stem Cells / Q000166:cytology; D018613:Microscopy, Confocal; D032383:Myocytes, Cardiac / Q000166:cytology; D018408:Patch-Clamp Techniques; D051381:Rats
chemical_list: D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; C013830:baysilon