title: Targeted multifunctional gold-based nanoshells for magnetic resonance-guided laser ablation of head and neck cancer.
abstract: Image-guided thermal ablation of tumors is becoming a more widely accepted minimally invasive alternative to surgery for patients who are not good surgical candidates, such as patients with advanced head and neck cancer. In this study, multifunctional superparamagnetic iron oxide coated with gold nanoshell (SPIO@Au NS) that have both optical and magnetic properties was conjugated with the targeting agent, C225 monoclonal antibody, against epidermal growth factor receptor (EGFR). C225-SPIO@Au NS have an average a diameter of 82 ± 4.4 nm, contain 142 ± 15 antibodies per nanoshell, have an absorption peak in the near infrared (~800 nm), and have transverse relaxivity (r(2)) of 193 and 353 mM(-1) s(-1) versus Feridex™ of 171 and 300 mM(-1) s(-1), using 1.5 T and 7 T MR scanners, respectively. Specific targeting of the synthesized C225-SPIO@Au NS was tested in vitro using A431 cells and oral cancer cells, FaDu, OSC19, and HN5, all of which overexpress EGFR. Selective binding was achieved using C225-SPIO@Au NS but not with the non-targeting PEG-SPIO@Au NS and blocking group (excess of C225 + C225-SPIO@Au NS). In vivo biodistribution on mice bearing A431 tumors also showed selective targeting of C225-SPIO@Au NS compared with the non-targeting and blocking groups. The selective photothermal ablation of the nanoshells shows that without laser treatment there were no cell death and among the groups that were treated with laser at a power of 36 W/cm(2) for 3 min, only the cells treated with C225-SPIO@Au NS had cell killing (p < 0.001). In summary, successful synthesis and characterization of targeted C225-SPIO@Au NS demonstrating both superparamagnetic and optical properties has been achieved. We have shown both in vitro and in vivo that these nanoshells are MR-active and can be selectively heated up for simultaneous imaging and photothermal ablation therapy.
authors: Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
authors: Marites P
authors: MP
authors: Melancon
authors: Wei
authors: W
authors: Lu
authors: Meng
authors: M
authors: Zhong
authors: Min
authors: M
authors: Zhou
authors: Gan
authors: G
authors: Liang
authors: Andrew M
authors: AM
authors: Elliott
authors: John D
authors: JD
authors: Hazle
authors: Jeffrey N
authors: JN
authors: Myers
authors: Chun
authors: C
authors: Li
authors: R Jason
authors: RJ
authors: Stafford
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry / Q000627:therapeutic use; D006046:Gold / Q000737:chemistry* / Q000627:therapeutic use*; D006258:Head and Neck Neoplasms / Q000628:therapy*; D006801:Humans; D053685:Laser Therapy / Q000379:methods*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D057145:Nanoshells / Q000737:chemistry* / Q000627:therapeutic use*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D006046:Gold; D066246:ErbB Receptors