title: Screening method for the analysis of antiviral drugs in poultry tissues using zwitterionic hydrophilic interaction liquid chromatography/tandem mass spectrometry.
abstract: A screening method for the analysis of seven anti-viral drugs in poultry tissue has been developed. These include anti-influenza drugs (amantadine, rimantadine, zanamivir and oseltamivir and its carboxylate metabolite), anti-herpes drugs (acyclovir and ganciclovir) and an immunomodulator (imiquimod). Poultry tissue was extracted in acetonitrile:water:acetic acid. After sample purification, using a strong cation exchange column, the eluate was split into two fractions. The first portion was dissolved in methanol:water and the second in acetonitrile:methanol:water. Both fractions were analysed on a zwitterionic hydrophilic interaction liquid chromatography column coupled to a triple quadrupole mass spectrometer. The screening method was successfully validated according to Commission Decision 2002/657/EC in three different laboratories with CCβ concentrations of ≤10 μg kg(-1).
authors: Food and Environment Research Agency, Sand Hutton, York, UK. danny.chan@fera.gsi.gov.uk
authors: D
authors: D
authors: Chan
authors: J
authors: J
authors: Tarbin
authors: M
authors: M
authors: Sharman
authors: M
authors: M
authors: Carson
authors: M
authors: M
authors: Smith
authors: S
authors: S
authors: Smith
mesh_terms: D000276:Adjuvants, Immunologic / Q000032:analysis / Q000302:isolation & purification; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D011200:Poultry; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000276:Adjuvants, Immunologic; D000998:Antiviral Agents