title: Cell migration in confinement: a micro-channel-based assay.
abstract: This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages even if it does not recapitulate the full complexity of 3D migration. Important parameters such as degree of adhesion, degree of confinement, mechanical properties, and geometry can be varied independently of each other. The device is fully compatible with almost any type of light microscopy and the simple geometry makes automated analysis very easy to perform, which allows screening strategy. The chapters is divided into five parts describing the design of different types of migration chambers, the fabrication of a mold by photolithography, the assembly of the chamber, the loading of cells, and finally the imaging on live or fixed cells.
authors: Subcellular Structure and Cellular Dynamics, Institut Curie, Paris, Cedex, France.
authors: Mélina L
authors: ML
authors: Heuzé
authors: Olivier
authors: O
authors: Collin
authors: Emmanuel
authors: E
authors: Terriac
authors: Ana-Maria
authors: AM
authors: Lennon-Duménil
authors: Matthieu
authors: M
authors: Piel
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D018929:Cell Culture Techniques; D054443:Cell Migration Assays / Q000295:instrumentation / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D012825:Silicon / Q000737:chemistry; D059010:Single-Cell Analysis; D059008:Time-Lapse Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D012825:Silicon