title: Experimental depression induces renal oxidative stress in rats.
abstract: Depression has been associated to inflammatory and oxidative events. Previous report has shown renal oxidative stress in patients with depression. In order to analyze if depressive status is related to renal oxidative and inflammatory events, Sprague Dawley rats were submitted to forced swimming test (FST) and the renal oxidative metabolism, monocyte-macrophage infiltration and Angiotensin II (Ang II) expression were determined. Rats were submitted to FST daily (30 min) for 15 days. Motor activity was analyzed before FST. Kidney sections were homogenized to measure nitric oxide (NO), malondialdehyde (MDA), reduced glutathione (GSH) and catalase activity by enzymatic and biochemical methods. Renal frozen sections were studied for superoxide anion (O2-), monocyte/macrophage infiltration and Ang II expression by histochemical and immunofluorescence methods. In addition, three groups of FST rats were treated with losartan, sertraline or water for 18 days with further renal O2-analysis. In the FST group, struggle time, motor activity, food intake and body weight gain were found decreased. Increased number of glomerular, interstitial and tubular O2-positive cells was observed in FST rats. High renal content of nitrite/nitrate (NO), MDA and decreased amount of GSH were found in FST rats. Values of renal ED-1 or Ang II positive cells in FST rats remained similar to controls; however, AT1 receptor blocking (losartan) and sertraline reduced both depressive-like behavior and renal O2-expression. These data suggests that depression-like behavior in rats is involved in kidney oxidative stress probably mediated by AT1 receptors.
authors: Catedra de Inmunologia, Escuela de Bioanalisis, Facultad de Medicina, Universidad del Zulia, Venezuela.
authors: Adriana
authors: A
authors: Pedreañez
authors: José Luis
authors: JL
authors: Arcaya
authors: Edgardo
authors: E
authors: Carrizo
authors: Jaimar
authors: J
authors: Rincón
authors: Ninoska
authors: N
authors: Viera
authors: Caterina
authors: C
authors: Peña
authors: Renata
authors: R
authors: Vargas
authors: Jesús
authors: J
authors: Mosquera
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D003863:Depression / Q000473:pathology* / Q000503:physiopathology*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D009043:Motor Activity / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism; D013550:Swimming / Q000523:psychology; D013997:Time Factors
chemical_list: D013481:Superoxides; D000804:Angiotensin II; D009569:Nitric Oxide; D008315:Malondialdehyde; D005978:Glutathione