title: A novel function for a redox-related LEA protein (SAG21/AtLEA5) in root development and biotic stress responses.
abstract: SAG21/AtLEA5 belongs to the late embryogenesis-associated (LEA) protein family. Although it has been implicated in growth and redox responses, its precise roles remain obscure. To address this problem, we characterized root and shoot development and response to biotic stress in SAG21/AtLEA5 over-expressor (OEX) and antisense (AS) lines. AS lines exhibited earlier flowering and senescence and reduced shoot biomass. Primary root length was reduced in AS lines, as was the number of laterals relative to the primary root. Root hair number was unchanged but root hair length was proportional to SAG21/AtLEA5 expression level, with longer root hairs in OEX lines and shorter root hairs in AS, relative to wild type. Growth of the fungal nectroph, Botrytis cinerea and of a virulent bacterial pathogen (Pseudomonas syringae pv. tomato) was affected by SAG21/AtLEA5 expression; however, growth of an avirulent P.syringae strain was unaffected. A SAG21/AtLEA5-YFP fusion was localized to mitochondria, raising the intriguing possibility that SAG21 interacts with proteins involved in mitochondrial ROS signalling, which in turn, impacts on root development and pathogen responses.
authors: School of Biological Sciences, Cardiff University, Cardiff, CF10 3TL, UK.
authors: Faezah Mohd
authors: FM
authors: Salleh
authors: Karly
authors: K
authors: Evans
authors: Benjamin
authors: B
authors: Goodall
authors: Helena
authors: H
authors: Machin
authors: Shaheen B
authors: SB
authors: Mowla
authors: Luis A J
authors: LA
authors: Mur
authors: John
authors: J
authors: Runions
authors: Frederica L
authors: FL
authors: Theodoulou
authors: Christine H
authors: CH
authors: Foyer
authors: Hilary J
authors: HJ
authors: Rogers
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000382:microbiology / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D020171:Botrytis / Q000254:growth & development; D016922:Cellular Senescence; D005786:Gene Expression Regulation / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D009928:Organ Specificity; D010084:Oxidation-Reduction; D010641:Phenotype; D035261:Plant Components, Aerial / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000254:growth & development / Q000502:physiology; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development; D017382:Reactive Oxygen Species / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D036226:Seedlings / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology*; D013997:Time Factors
chemical_list: D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D011993:Recombinant Fusion Proteins; C000588750:SAG21 protein, Arabidopsis