title: Mapping functional domains of transcription factors.
abstract: Transcription factors are modular in nature in all organisms. In general, they have a DNA binding domain, one or more transcription activation and/or repressor domain, and often a dimerization domain. In many cases, transcription factors also have other protein-protein interaction domain(s). Mapping these functional domains in transcription factors is critical in understanding their molecular function. In this chapter, protocols for mapping the DNA binding domain and the transcription activation domain of a bHLH class of transcription factor are described. In principle, these protocols can be applied to other classes of transcription factors for mapping their functional domains.
authors: Section of Molecular Cell and Developmental Biology, Institute for Cellular and Molecular Biology, The University of Texas, Austin, TX, USA. lingzhu@mail.utexas.edu
authors: Ling
authors: L
authors: Zhu
authors: Enamul
authors: E
authors: Huq
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D010449:Peptide Mapping / Q000379:methods*; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D025941:Protein Interaction Mapping / Q000379:methods*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors