title: Detection and isolation of p-nitrophenol-lowering bacteria from intestine of marine fishes caught in Japanese waters.
abstract: To determine the existence of p-nitrophenol (PNP)-lowering bacteria in intestine of Japanese coastal fish, the gastro-intestinal contents were incubated in Brain Heart Infusion (BHI) broth and minimal medium (MM) broths containing 1 mmol/L PNP at 30 °C for 7 days. Among 26 samples of 19 fish species, 17 samples showed a decrease in PNP of 0.5-0.8 mmol/L in BHI broth, but no decrease was shown in MM broth. Eighteen PNP-lowering bacterial strains were isolated from four fishes. All of the strains were identified as Lactococcus lactis subsp. lactis. Three L. lactis strains JS1-3 isolated from Japanese seabass Lateolabrax japonicus showed the highest PNP-lowering activity (0.44 mmol/L). Optimum temperature and pH for the growth and PNP decreasing corresponded with the marine environment. These results suggested that marine fishes have PNP decreasing bacteria in their intestine. These bacteria might protect host fish from toxicities of PNP and PNP related compounds.
authors: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan. kuda@kaiyodai.ac.jp
authors: Takashi
authors: T
authors: Kuda
authors: Daisuke
authors: D
authors: Kyoi
authors: Hajime
authors: H
authors: Takahashi
authors: Kazuhiro
authors: K
authors: Obama
authors: Bon
authors: B
authors: Kimura
mesh_terms: D000818:Animals; D005399:Fishes / Q000145:classification / Q000378:metabolism* / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D007564:Japan; D013294:Lactococcus lactis / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D008386:Marine Biology; D009596:Nitrophenols / Q000378:metabolism*; D013696:Temperature
chemical_list: D009596:Nitrophenols; C024836:4-nitrophenol