title: Prevalence of integrons, blaCTX-M and blaTEM resistance markers among ESBL-producing uropathogenic Escherichia coli isolates: first report of genomic blaCTX-M from India.
abstract: Integrons have been observed to be frequently associated with uropathogenic bacteria. This study aimed at 1) determining the prevalence of class 1 integrons among ESBLl-producing uropathogenic Escherichia coli, and 2) analyzing resistance genes associated with different phylogenetic groups of the integron-positive isolates with special reference to bla(CTX-M) and bla(TEM). Twenty-three ESBL-producing E. coli were studied. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) displayed 14 major patterns. Pulse field Gel electrophoresis-typing of 8 randomly selected integron-positive strains ruled out any correlation between genotype and antibiotype. Genomic DNA from 14 strains was PCR-positive for class 1 integrons, bla(CTX-M-15) and bla(TEM-1)-like genes. Integron-sequencing revealed "aadA5-dfrA17-dfrA7" as the most prevalent gene cassette. Our findings unveil the increasing role of the bla(CTX-M) genes in antibiotic resistance and emphasize on the significance of appropriate empirical treatment for Urinary tract infections. Moreover, this is the first study which reports bla(CTX-M) located on genomic DNA of bacteria from India.
authors: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.
authors: M
authors: M
authors: Akram
authors: S
authors: S
authors: Shakil
authors: A U
authors: AU
authors: Khan
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D019450:Genes, MDR; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D007194:India; D032023:Integrons; D010802:Phylogeny; D015995:Prevalence; D014552:Urinary Tract Infections / Q000188:drug therapy / Q000382:microbiology; D056689:Uropathogenic Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases