title: Impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cutaneous wound healing.
abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a representative of a large group of polyhalogenated aromatic hydrocarbons that are widespread environmental contaminants. Administration of TCDD to laboratory animals or cultured cells results in a number of adverse effects that are well documented. For example, the effects of TCDD observed in developing organisms indicate that exposure to this class of environmental contaminants significantly alters embryo morphogenesis. However, it is not clear whether tissue regeneration in adult animals may be similarly affected. With this in mind, we examined the impact of TCDD exposure on wound healing using a murine cutaneous wound healing model. Our results indicate that TCDD exposure did not significantly alter the time needed for wound closure. However, in the TCDD-treated mice, a significant decrease in tensile strength in the healed wounds was observed which is indicative of an aberrantly healed wound. Immunostaining revealed that exposure to TCDD increased the population of macrophages detected within the wounded tissue at the latter stages of wound healing. Our findings support the idea that exposure to environmental contaminants such as TCDD is proinflammatory in the wounded tissue, disrupts normal healing and ultimately produces in a poorly healed wound.
authors: Department of Molecular and Biomedical Pharmacology, College of Medicine, University of Kentucky, MS 305, Lexington, KY 40536, United States.
authors: Valentina
authors: V
authors: Moirangthem
authors: Wendy S
authors: WS
authors: Katz
authors: Wen
authors: W
authors: Su
authors: Eun-Young
authors: EY
authors: Choi
authors: R W Cameron
authors: RW
authors: Dingle
authors: Georgia M
authors: GM
authors: Zeigler
authors: William V
authors: WV
authors: Everson
authors: C Darrell
authors: CD
authors: Jennings
authors: Ming
authors: M
authors: Gong
authors: Hollie I
authors: HI
authors: Swanson
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000293:injuries / Q000473:pathology; D013718:Tensile Strength; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D019363:Cytochrome P-450 CYP1A1