title: Glycomic analysis of human mast cells, eosinophils and basophils.
abstract: In allergic diseases such as asthma, eosinophils, basophils and mast cells, through release of preformed and newly generated mediators, granule proteins and cytokines, are recognized as key effector cells. While their surface protein phenotypes, mediator release profiles, ontogeny, cell trafficking and genomes have been generally explored and compared, there has yet to be any thorough analysis and comparison of their glycomes. Such studies are critical to understand the contribution of carbohydrates to the induction and regulation of allergic inflammatory responses and are now possible using improved technologies for detecting and characterizing cell-derived glycans. We thus report here the application of high-sensitivity mass spectrometric-based glycomics methodologies to the analysis of N-linked glycans derived from isolated populations of human mast cells, eosinophils and basophils. The samples were subjected to matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) screening analyses and MALDI-TOF/TOF sequencing studies. Results reveal substantive quantities of terminal N-acetylglucosamine containing structures in both the eosinophil and the basophil samples, whereas mast cells display greater relative quantities of sialylated terminal epitopes. For the first time, we characterize the cell surface glycan structures of principal allergic effector cells, which by interaction with glycan-binding proteins (e.g. lectins) have the possibility to dictate cellular functions, and might thus have important implications for the pathogenesis of inflammatory and allergic diseases.
authors: Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
authors: Simon J
authors: SJ
authors: North
authors: Stephan
authors: S
authors: von Gunten
authors: Aristotelis
authors: A
authors: Antonopoulos
authors: Alana
authors: A
authors: Trollope
authors: Donald W
authors: DW
authors: MacGlashan
authors: Jihye
authors: J
authors: Jang-Lee
authors: Anne
authors: A
authors: Dell
authors: Dean D
authors: DD
authors: Metcalfe
authors: Arnold S
authors: AS
authors: Kirshenbaum
authors: Bruce S
authors: BS
authors: Bochner
authors: Stuart M
authors: SM
authors: Haslam
mesh_terms: D001491:Basophils / Q000378:metabolism*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D004804:Eosinophils / Q000378:metabolism*; D054794:Glycomics; D006801:Humans; D008407:Mast Cells / Q000378:metabolism*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid