title: Gene expression in Trypanosoma brucei: lessons from high-throughput RNA sequencing.
abstract: Trypanosoma brucei undergoes major biochemical and morphological changes during its development from the bloodstream form in the mammalian host to the procyclic form in the midgut of its insect host. The underlying regulation of gene expression, however, is poorly understood. More than 60% of the predicted genes remain annotated as hypothetical, and the 5' and 3' untranslated regions important for regulation of gene expression are unknown for >90% of the genes. In this review, we compare the data from four recently published high-throughput RNA sequencing studies in light of the different experimental setups and discuss how these data can enhance genome annotation and give insights into the regulation of gene expression in T. brucei.
authors: Biology of Host-Parasite Interactions, Parasitology, Institut Pasteur, 75724 Paris, France.
authors: T Nicolai
authors: TN
authors: Siegel
authors: Kapila
authors: K
authors: Gunasekera
authors: George A M
authors: GA
authors: Cross
authors: Torsten
authors: T
authors: Ochsenreiter
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D026723:Polyadenylation; D022821:RNA Splice Sites; D012326:RNA Splicing; D020871:RNA Stability; D017423:Sequence Analysis, RNA / Q000379:methods; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014370:Tsetse Flies / Q000469:parasitology; D020506:Untranslated Regions
chemical_list: D022821:RNA Splice Sites; D020506:Untranslated Regions