title: Poly(I:C) induce bone marrow precursor cells into myeloid-derived suppressor cells.
abstract: Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MDSC with LPS plus IFN-γ inhibited the DC development but enhanced MDSC functions, such as NO release and T cell suppression. In our study, bone marrow precursor cells cultures in GM-CSF and IL-4 were treated with poly(I:C) through the culture, Gr1(+)CD11b(+) cells with MDSC functions, such as NO release and T cell suppression were accumulated in the culture system. Then the similar phenomenon was observed in the vesicular stomatitis virus infection in vivo. In conclusion, we demonstrated that the bone marrow precursor cells in the presence of GM-CSF and IL-4 can differentiate into MDSC, which is dependent on the dynamic of interaction with poly(I:C).
authors: National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai, China.
authors: Cong
authors: C
authors: Liu
authors: Chaoxiong
authors: C
authors: Zhang
authors: Hongjuan
authors: H
authors: Lu
authors: Jianming
authors: J
authors: Cai
authors: Zhigang
authors: Z
authors: Wang
authors: Jie
authors: J
authors: Chen
authors: Fang
authors: F
authors: Liu
authors: Zhumei
authors: Z
authors: Wu
authors: Xiaogang
authors: X
authors: Liu
authors: Weimin
authors: W
authors: Sun
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D022423:Myeloid Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D010641:Phenotype; D011070:Poly I-C / Q000494:pharmacology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000821:virology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D018116:Vesiculovirus / Q000502:physiology
chemical_list: D039481:CD11b Antigen; D016207:Cytokines; D011070:Poly I-C