title: Bromopyridazinedione-mediated protein and peptide bioconjugation.
abstract: Bromopyridazinedione-mediated bioconjugation to a cysteine containing protein and a disulfide containing peptide is described. The conjugates are cleavable in an excess of thiol, including cytoplasmically-relevant concentrations of glutathione, and show a high level of hydrolytic stability. The constructs have the potential for four points of chemical attachment.
authors: Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
authors: Vijay
authors: V
authors: Chudasama
authors: Mark E B
authors: ME
authors: Smith
authors: Felix F
authors: FF
authors: Schumacher
authors: Danai
authors: D
authors: Papaioannou
authors: Gabriel
authors: G
authors: Waksman
authors: James R
authors: JR
authors: Baker
authors: Stephen
authors: S
authors: Caddick
mesh_terms: D003545:Cysteine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011724:Pyridazines / Q000737:chemistry*
chemical_list: D004220:Disulfides; D010455:Peptides; D011506:Proteins; D011724:Pyridazines; C062482:pyridazine; D005978:Glutathione; D003545:Cysteine