title: Analysis of different medulloblastoma histotypes by two-dimensional gel and MALDI-TOF.
abstract: OBJECTIVE
  The purpose of this study is to detect different protein profiles in medulloblastoma (MDB) that may be clinically relevant and to check the correspondence of histological classification of MDB with proteomic profiles.
  
  
  METHODS
  Surgical specimens, snap frozen at the time of neurosurgery, entered the proteomic study. Eight samples from patients (age range, 4 months-26 years) with different MDB histotypes (five classic, one desmoplastic/nodular, one with extensive nodularity, and one anaplastic) were analyzed by two-dimensional gel electrophoresis. One sample for each histotype was further characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis.
  
  
  RESULTS
  Eighty-six unique proteins were identified and compared to histology, with the determination of proteins expressed by single histotypes and of a smaller number of proteins shared by two or three histotypes. The sharp difference of protein expression was found to be in agreement with WHO histological classification, with the identification of type-specific proteins with limited overlapping between histotypes.
  
  
  CONCLUSIONS
  Proteomic analysis confirmed and strengthened the difference between histotypes as biologically relevant. Cluster analysis enhanced the distance of extensive nodularity MDB from other histotypes. Possible innovative approaches to therapy may rely upon a proteomic-based classification of MDB tightly correlated to histology. The utility of snap freezing tumoral samples must be stressed and should become a mandatory task for pathologists.
authors: Molecular Biotechnology Centre (MBC), University of Turin, Via Nizza 52. CAP, 10126 Torino, Italy. cristina.zanini@unito.it
authors: Cristina
authors: C
authors: Zanini
authors: Giorgia
authors: G
authors: Mandili
authors: Daniele
authors: D
authors: Bertin
authors: Francesco
authors: F
authors: Cerutti
authors: Denisa
authors: D
authors: Baci
authors: Marco
authors: M
authors: Leone
authors: Isabella
authors: I
authors: Morra
authors: Luca
authors: L
authors: di Montezemolo Cordero
authors: Marco
authors: M
authors: Forni
mesh_terms: D000293:Adolescent; D000328:Adult; D002528:Cerebellar Neoplasms / Q000145:classification / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008527:Medulloblastoma / Q000145:classification / Q000378:metabolism*; D054464:Peroxiredoxins / Q000032:analysis / Q000378:metabolism; D011506:Proteins / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D050777:Stathmin / Q000032:analysis / Q000378:metabolism; D055815:Young Adult
chemical_list: D011506:Proteins; D050777:Stathmin; C086065:PRDX2 protein, human; D054464:Peroxiredoxins