title: Is p53 controlling spermatogenesis in male mice with the deletion on the Y chromosome?
abstract: The aim of the study was to evaluate the influence of the chromosome Y structure and Trp53 genotype on semen quality parameters. Mice with partial deletion of the Y chromosome (B10.BR-Ydel) have severely altered sperm head morphology when compared with males that possess the complete Y chromosome (B10.BR). Control males from B10.BR and B10.BR-Ydel mice, and mutant males from B10.BR-p53 -/- and B10.BR-Ydel-p53 -/- experimental groups were used. We assessed testis weight, sperm head abnormalities, viability of spermatozoa (eosin test), percentage of motile and immature sperm, and performed a hypo-osmotic test to detect abnormal tail membrane integrity. Sperm morphology and maturation were controlled by the genes within the deleted region of the Y chromosome. Testis weight was higher in the mutants than in the control males, possibly due to cell accumulation in Trp53-deficient males as the concentration of sperm was significantly increased in the mutants. An elevated percentage of abnormal sperm was noted in B10.BR-p53 -/- and B10.BR-Ydel-p53 -/- male mice. We suggest that, in Trp53-deficient mice, the sperm cells that escape apoptosis are the ones that have abnormal morphology. The only sperm quality parameter affected by the interplay between Trp53 and chromosome Y genes was sperm motility, which was elevated in B10.BR-p53 -/- males, but remained unchanged in B10.BR-Ydel-p53 -/- males.
authors: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Cracow, Poland.
authors: Tomasz
authors: T
authors: Lech
authors: Aniela
authors: A
authors: Golas
authors: Jozefa
authors: J
authors: Styrna
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002872:Chromosome Deletion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009929:Organ Size / Q000235:genetics; D055101:Semen Analysis; D013077:Sperm Head / Q000473:pathology; D013081:Sperm Motility / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000033:anatomy & histology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014998:Y Chromosome
chemical_list: D016159:Tumor Suppressor Protein p53