title: Backbone and side chain NMR resonance assignments for an archaeal homolog of the endonuclease Nob1 involved in ribosome biogenesis.
abstract: Eukaryotic ribosome biogenesis requires the concerted action of ~200 auxiliary protein factors on the nascent ribosome. For many of these factors structural and functional information is still lacking. The endonuclease Nob1 has been recently identified in yeast as the enzyme responsible for the final cytoplasmatic trimming step of the pre-18S rRNA during the biogenesis of the small ribosomal subunit. Here we report the NMR resonance assignments for a Nob1 homolog from the thermophilic archeon Pyrococcus horikoshii as a prerequisite for further structural studies of this class of proteins.
authors: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany.
authors: Thomas
authors: T
authors: Veith
authors: Jan Philip
authors: JP
authors: Wurm
authors: Elke
authors: E
authors: Duchardt-Ferner
authors: Benjamin
authors: B
authors: Weis
authors: Roman
authors: R
authors: Martin
authors: Charlotta
authors: C
authors: Safferthal
authors: Markus T
authors: MT
authors: Bohnsack
authors: Enrico
authors: E
authors: Schleiff
authors: Jens
authors: J
authors: Wöhnert
mesh_terms: D004720:Endonucleases / Q000737:chemistry* / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D043902:Pyrococcus horikoshii / Q000166:cytology* / Q000201:enzymology*; D012270:Ribosomes / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013696:Temperature
chemical_list: D004720:Endonucleases