title: Attenuation of 1-(m-chlorophenyl)-biguanide induced hippocampus-dependent memory impairment by a standardised extract of Bacopa monniera (BESEB CDRI-08).
abstract: Bacopa monniera is a well-known medhya-rasayana (memory enhancing and rejuvenating) plant in Indian traditional medical system of Ayurveda. The effect of a standardized extract of Bacopa monniera (BESEB CDRI-08) on serotonergic receptors and its influence on other neurotransmitters during hippocampal-dependent learning was evaluated in the present study. Wistar rat pups received a single dose of BESEB CDRI-08 during postnatal days 15-29 showed higher latency during hippocampal-dependent learning accompanied with enhanced 5HT(3A) receptor expression, serotonin and acetylcholine levels in hippocampus. Furthermore, 5HT(3A) receptor agonist 1-(m-chlorophenyl)-biguanide (mCPBG) impaired learning in the passive avoidance task followed by reduction of 5HT(3A) receptor expression, 5HT and ACh levels. Administration of BESEB CDRI-08 along with mCPBG attenuated mCPBG induced behavioral, molecular and neurochemical alterations. Our results suggest that BESEB CDRI-08 possibly acts on serotonergic system, which in turn influences the cholinergic system through 5-HT(3) receptor to improve the hippocampal-dependent task.
authors: Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India. emmanuvel1972@yahoo.com
authors: Koilmani Emmanuvel
authors: KE
authors: Rajan
authors: Hemant K
authors: HK
authors: Singh
authors: Arunagiri
authors: A
authors: Parkavi
authors: Prisila Dulcy
authors: PD
authors: Charles
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D034681:Bacopa / Q000737:chemistry; D001645:Biguanides / Q000633:toxicity*; D005260:Female; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D008297:Male; D008515:Medicine, Ayurvedic; D008568:Memory / Q000187:drug effects*; D008569:Memory Disorders / Q000188:drug therapy / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D044406:Receptors, Serotonin, 5-HT3 / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism
chemical_list: C569478:BESEB CDRI-08; D001645:Biguanides; D010936:Plant Extracts; D044406:Receptors, Serotonin, 5-HT3; D012701:Serotonin; C065734:1-(3-chlorophenyl)biguanide; D000109:Acetylcholine