title: Novel cytotoxity exhibition mode of polybia-CP, a novel antimicrobial peptide from the venom of the social wasp Polybia paulista.
abstract: Antimicrobial peptide polybia-CP was purified from the venom of the social wasp Polybia paulista. It has an amphipathic sequence ILGTILGLLKSL-NH(2) and possesses potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. In this study we synthesized polybia-CP, studied its cytotoxity on tumor cells and proposed its possible mechanism. Our results revealed that polybia-CP exerts its cytotoxic efficacy by disrupting the integrity of cell membrane. Furthermore, molecular dynamics (MD) simulations were employed to investigate the mechanism of membrane perturbation. Both the MD simulations and the experimental data indicated that polybia-CP takes a standard α-helix conformation in the membrane. These findings together with the other experimental results support a speculation of mechanism similar to the "carpet" model.
authors: Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Institute of Biochemistry and Molecular Biology and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, 222 Tian Shui South Road, Lanzhou 730000, PR China. wangkr@lzu.edu.cn
authors: Kairong
authors: K
authors: Wang
authors: Jiexi
authors: J
authors: Yan
authors: Xin
authors: X
authors: Liu
authors: Jindao
authors: J
authors: Zhang
authors: Ru
authors: R
authors: Chen
authors: Bangzhi
authors: B
authors: Zhang
authors: Wen
authors: W
authors: Dang
authors: Wei
authors: W
authors: Zhang
authors: Ming
authors: M
authors: Kai
authors: Jingjing
authors: J
authors: Song
authors: Rui
authors: R
authors: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D006801:Humans; D008297:Male; D056004:Molecular Dynamics Simulation; D011471:Prostatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D011487:Protein Conformation; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014862:Wasp Venoms / Q000737:chemistry*; D014863:Wasps
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D014862:Wasp Venoms