title: Detection of urease-negative Bordetella bronchiseptica from the field.
abstract: Four urease-negative Bordetella bronchiseptica isolates originating from pigs were examined by phenotypic and molecular methods. The phenotypic properties of the isolates were in harmony with the data of the literature, except for the lack of urease activity in conventional tube test, API 20 NE and Diatabs™ assays. Using genotypic methods, the urease-negative isolates did not differ from the urease-positive reference strain. They were positive in species-specific and ureC PCR, and all strains showed uniform bands in PCR-RFLP studies of flaA genes. The reason for the lack of urease activity, a characteristic considered species specific for B. bronchiseptica, needs to be studied further. The finding underlines the significance of genotyping when the phenotypic identification of B. bronchiseptica seems questionable.
authors: Veterinary Medical Research Institute of the Hungarian Academy of Sciences Hungária krt, Budapest,Hungary.
authors: Bernadett
authors: B
authors: Khayer
authors: Zsuzsanna
authors: Z
authors: Rónai
authors: Eniko
authors: E
authors: Wehmann
authors: Tibor
authors: T
authors: Magyar
mesh_terms: D000818:Animals; D001885:Bordetella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016950:Bordetella bronchiseptica / Q000201:enzymology* / Q000235:genetics*; D006814:Hungary / Q000453:epidemiology; D012222:Rhinitis, Atrophic / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D014510:Urease / Q000235:genetics / Q000378:metabolism*
chemical_list: D014510:Urease