title: Capture of dengue virus type 3 using anionic polymer-coated magnetic beads.
abstract: Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available detection methods. We here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). The beads were incubated with cell culture medium of DENV-3-infected mosquito cells, then separated from the supernatant by applying a magnetic field and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase chain reaction, which detected the presence of DENV-3 genomic RNA on the beads, and Western blotting, which determined the major DENV-3 envelope protein on the beads. Therefore, this capture method may enable an improvement in DENV-3 detection.
authors: Department of Virology, Center for Infectious Disease Control, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan. sakudo@med.u-ryukyu.ac.jp
authors: Akikazu
authors: A
authors: Sakudo
authors: Promsin
authors: P
authors: Masrinoul
authors: Yasuharu
authors: Y
authors: Tanaka
authors: Kazuyoshi
authors: K
authors: Ikuta
mesh_terms: D000330:Aedes / Q000821:virology; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003716:Dengue Virus / Q000302:isolation & purification*; D008280:Magnetics; D011108:Polymers / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011108:Polymers