title: [Promoter methylation status of hPer3 gene in AML patients and the in vitro effect of decitabine on the status].
abstract: OBJECTIVE
  To investigate the clinical significance of promoter methylation status of hPer3 gene in acute myeloid leukemia (AML) patients and the in vitro effect of decitabine (DCA) on AML cell lines HL-60 and U937.
  
  
  METHODS
  The promoter methylation status of hPer3 gene and mRNA expression levels in bone marrow of 206 AML and 40 iron deficiency anemia (IDA) patients (as control) were detected by methylation specific PCR (MS-PCR) and real-time PCR (RT-PCR). The HL-60 and U937 cell lines were treated with different concentrations of DCA for 48 and 72 h. The inhibition rates of cell proliferation were detected by methyl thiazolyl tetrazolium (MTT); the early apoptosis rates by staining with Annexin V and PI; the CD14 and CD11b expressions by flow cytometry (FCM); the promoter methylation status of hPer3 gene by MS-PCR; and the hPer3 mRNA expressions levels by RT-PCR.
  
  
  RESULTS
  The promoter methylation rates of hPer3 in newly diagnosed (ND) group, partial remission(PR) group, complete remission (CR) group, relapse (R) group and control group were 93.65% (59/63), 54.39% (31/57), 24.66% (18/73), 61.54% (8/13) and 0% (0/40), and the hPer3 mRNA expression levels were 0.19 ± 0.08, 6.28 ± 2.11, 52.76 ± 14.17, 8.18 ± 4.36, 75.03 ± 18.16, respectively. There was a significant statistic difference between any two group (P < 0.01) excepting for between PR and R group (P > 0.05). After DCA treatment, the promoter hypermethylation status of hPer3 was reduced and the mRNA and CD14, CD11b expression levels were up regulated in a dose dependent manner with an induction of cell apoptosis.
  
  
  CONCLUSIONS
  Promotor methylation status and mRNA expression of hPer3 gene may be indicators for evaluating AML. DCA can induce the expression of hPer3 gene and cells apoptosis in AML.
authors: Zhoushan Hospital, Zhoushan 316004, China.
authors: Ye-kai
authors: YK
authors: Wang
authors: Ji-hang
authors: JH
authors: Zhou
authors: Shi-quan
authors: SQ
authors: Zhou
authors: Guo-an
authors: GA
authors: Fang
authors: Yi-wei
authors: YW
authors: Li
authors: Lei
authors: L
authors: Qiu
authors: Xu-chun
authors: XC
authors: Yang
authors: Xiao-guang
authors: XG
authors: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D049109:Cell Proliferation; D019175:DNA Methylation; D000077209:Decitabine; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D056950:Period Circadian Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D020298:U937 Cells; D055815:Young Adult
chemical_list: C512644:PER3 protein, human; D056950:Period Circadian Proteins; D000077209:Decitabine; D001374:Azacitidine