title: Identification of markers that distinguish IgE- from IgG-mediated anaphylaxis.
abstract: IgG-mediated anaphylaxis occurs in mice and may contribute to human reactions to infused drugs. To distinguish IgE- from putative IgG-mediated human anaphylaxis, we developed blood markers for murine anaphylaxis and evaluated their human relevance. Both IgG- and IgE-mediated anaphylaxis were characterized by decreased basophil and monocyte percentages and an increased neutrophil percentage in mouse blood. IgE- but not IgG-mediated murine anaphylaxis was accompanied by large increases in IL-4 secretion, plasma soluble IL-4 receptor-α (IL-4Rα) concentration, and T-cell membrane IL-4Rα expression. T-cell IL-4Rα expression also increased when mice that express human Fcε receptor Iα were sensitized with IgG-depleted serum from a peanut-allergic individual and challenged with peanut extract. Increased T-cell IL-4Rα expression is likely to also be a marker for human IgE-mediated anaphylaxis, because IgE-activated human basophils secrete IL-4, and IL-4 increases human T-cell IL-4Rα expression in vitro. Murine IgG- but not IgE-mediated anaphylaxis was characterized by decreased neutrophil Fcγ receptor III (FcγRIII) expression that was observed even when the antigen dose was insufficient to induce shock. Human neutrophils cultured with IgG immune complexes also lost FcγRIII. These observations suggest that decreased blood neutrophil FcγRIII expression without increased IL-4Rα expression can be used to determine whether and when IgG-mediated anaphylaxis occurs in man.
authors: Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220, USA.
authors: Marat V
authors: MV
authors: Khodoun
authors: Richard
authors: R
authors: Strait
authors: Laura
authors: L
authors: Armstrong
authors: Noriko
authors: N
authors: Yanase
authors: Fred D
authors: FD
authors: Finkelman
mesh_terms: D000707:Anaphylaxis / Q000097:blood / Q000145:classification / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D053818:Chymases / Q000097:blood; D004195:Disease Models, Animal; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009504:Neutrophils / Q000276:immunology; D021183:Peanut Hypersensitivity / Q000276:immunology; D011956:Receptors, Cell Surface / Q000097:blood; D017452:Receptors, IgG / Q000097:blood; D053662:Interleukin-4 Receptor alpha Subunit
chemical_list: D015415:Biomarkers; D053818:Chymases; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D015847:Interleukin-4; D011956:Receptors, Cell Surface; D017452:Receptors, IgG; C485700:Fcgr3 protein, mouse; C504002:Il4ra protein, mouse; D053662:Interleukin-4 Receptor alpha Subunit; C509230:Mcpt1 protein, mouse