title: Purification and characterization of a lectin with high hemagglutination property isolated from Allium altaicum.
abstract: A lectin was purified from the leaves of Allium altaicum and corresponding gene was cloned. The lectin namely Allium altaicum agglutinin (AAA) was ~24 kDa homodimeric protein and similar to a typical garlic leaf lectin. It was synthesized as 177 amino acid residues pre-proprotein, which consisted of 28 and 43 amino acid long N and C-terminal signal peptides, respectively. The plant expressed this protein more in scapes and flowers in comparison to the bulbs and leaves. Hemagglutination activity (with rabbit erythrocytes) was 1,428 fold higher as compared to Allium sativum leaf agglutinin (ASAL) although, the insecticidal activity against cotton aphid (Aphis gossypii) was relatively low. Glycan array revealed that AAA had higher affinity towards GlcAb1-3Galb as compared to ASAL. Homology analysis showed 57-94% similarity with other Allium lectins. The mature protein was expressed in E. coli as a fusion with SUMO peptide in soluble and biologically active form. Recombinant protein retained high hemagglutination activity.
authors: Council of Scientific and Industrial Research, National Botanical Research Institute, Rana Pratap Marg, Lucknow, Uttar Pradesh, India.
authors: Santosh Kumar
authors: SK
authors: Upadhyay
authors: Sharad
authors: S
authors: Saurabh
authors: Rahul
authors: R
authors: Singh
authors: Preeti
authors: P
authors: Rai
authors: Neeraj Kumar
authors: NK
authors: Dubey
authors: K
authors: K
authors: Chandrashekar
authors: Kuldeep Singh
authors: KS
authors: Negi
authors: Rakesh
authors: R
authors: Tuli
authors: P K
authors: PK
authors: Singh
mesh_terms: D000490:Allium / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000187:drug effects; D001483:Base Sequence; D002240:Carbohydrate Sequence; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli; D006384:Hemagglutination / Q000187:drug effects; D006388:Hemagglutinins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D025842:SUMO-1 Protein / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D053719:Tandem Mass Spectrometry
chemical_list: D006388:Hemagglutinins; D007306:Insecticides; D037121:Plant Lectins; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein