title: [In vivo and in vitro research study of the influence on placenta angiogenesis by gene silencing of netrin-1].
abstract: OBJECTIVE
  To study influence on angiogenesis of placenta by gene silencing of netrin-1.
  
  
  METHODS
  Netrin-1 gene in human umbilical vein endothelial cells (HUVEC) and placenta of pregnant rats were silenced by RNA interference. The following methods were used in this study, including the phenytetrazoliumromide (MTT) for viability, clone formation for proliferation, transwell for migration, and tube formation for angiogenesis in vitro. The change of fetal growth was recorded. Placental microvessel density in pregnant rats was measured by immunohistochemical CD(34) staining in vivo.
  
  
  RESULTS
  (1) HUVEC: viability and proliferation of HUVEC were remarkably inhibited by gene silencing of netrin-1, which number of clone formation, migration cell, tube formation were from (69 ± 6)%, 86 ± 17, 37 ± 9 decreased to (46 ± 5)%, 46 ± 13 and 17 ± 5 (P < 0.05) respectively. (2) Placenta of pregnant rats: after netrin-1 gene silenced, fetal weight were decreased from (2.39 ± 0.17) g to (2.12 ± 0.10) g (P < 0.05). Placental microvessel density was decreased from (258 ± 38)/mm(2) to (197 ± 32)/mm(2) in vivo (P < 0.05).
  
  
  CONCLUSIONS
  Gene silencing of netrin-1 could inhibit viability, proliferation, migration, tubal formation of HUVEC and angiogenesis of placenta. Netrin-1 plays an important role in regulating angiogenesis in placenta.
authors: Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors: Han
authors: H
authors: Xie
authors: Li
authors: L
authors: Zou
authors: Jian-wen
authors: JW
authors: Zhu
authors: Yun
authors: Y
authors: Yang
authors: Shao-ping
authors: SP
authors: Zhong
authors: Qian-hua
authors: QH
authors: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D020868:Gene Silencing; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000235:genetics* / Q000378:metabolism; D000075388:Netrin-1; D010920:Placenta / Q000098:blood supply* / Q000378:metabolism; D011247:Pregnancy; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C000617351:NTN1 protein, human; D009414:Nerve Growth Factors; C000617354:Ntn1 protein, rat; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1