title: In vitro induction and differentiation of newborn guinea pig hippocampus neural stem cells into cells resembling inner hair cells, using artificial perilymph.
abstract: OBJECTIVE
  To investigate whether artificial perilymph can induce neural stem cells, derived from the hippocampus of newborn guinea pigs, to differentiate into inner ear hair cells, in vitro.
  
  
  METHODS
  Primary neural stem cells derived from the hippocampus of newborn guinea pigs were incubated in medium containing either 10 per cent fetal bovine serum or 5, 10 or 15 per cent artificial perilymph, for three weeks. Differentiated cells were identified using immunofluorescence, Western blot and scanning electron microscopy.
  
  
  RESULTS
  Both fetal bovine serum and artificial perilymph induced the neural stem cells to differentiate into cells with hair-cell-specific antibodies.
  
  
  CONCLUSIONS
  Neural stem cells can survive in both fetal bovine serum and artificial perilymph, and within these media can differentiate into cells with hair-cell-specific antibodies. This provides an experimental basis for transplantation of neural stem cells into the inner ear.
authors: Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors: Y
authors: Y
authors: Wang
authors: M-M
authors: MM
authors: Dong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D008855:Microscopy, Electron, Scanning; D000081412:Myosin VIIa; D009218:Myosins / Q000032:analysis; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D010498:Perilymph; D010784:Photomicrography
chemical_list: D003470:Culture Media; D000081412:Myosin VIIa; D009218:Myosins