title: [The construction of NUDC shRNA interference vector with red fluorescence protein].
abstract: OBJECTIVE
  Used RFP gene to construct a RNA interference vector for convenience to obtain the good effective hairpins sequence.
  
  
  METHODS
  NUDC and RFP genes were cloned into pDs vector separately, resulting in pDs-NUDC- RFP. as above, human U6 promoter and 9 hairpins sequence of NUDC were cloned into the pDs- NUDC-RFP vector separately.The RNA interfererence vectors target to 9 points of NUDC were constructed. Construct- ed recombinant vectors and then were identified by restrictive digestion and DNA sequencing.293T cells were transfected with the recombinant DNA samples by the liposome complex method, and then fluorescence photographs were taken.
  
  
  RESULTS
  Enzyme digestion and DNA sequencing showed that the target gene and shRNA fragments were correctly inserted in pDs vector. fluorescence photographs showed that shNUDC-A is the best effective fragment.
  
  
  CONCLUSIONS
  The NUDC gene targeted shRNA and its vector are successfully constructed.
authors: Department of Biology of Guangdong Medical College, Zhanjiang, China.
authors: Xia
authors: X
authors: Liao
authors: Shi-feng
authors: SF
authors: Pang
authors: Qiang
authors: Q
authors: Zhang
authors: Fang
authors: F
authors: Yang
authors: Ke-qin
authors: KQ
authors: Zheng
authors: Ru-bin
authors: RB
authors: Zhou
authors: Ding-guo
authors: DG
authors: Cao
authors: Yu-guang
authors: YG
authors: Zou
mesh_terms: D019677:Artificial Gene Fusion / Q000379:methods*; D018797:Cell Cycle Proteins / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000172:deficiency* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D015183:Restriction Mapping; D000097573:Red Fluorescent Protein
chemical_list: D018797:Cell Cycle Proteins; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D000097573:Red Fluorescent Protein; C512648:NUDC protein, human