title: Isolation of detergent-resistant membranes from plant photosynthetic and non-photosynthetic tissues.
abstract: Microdomains, or lipid rafts, are transient membrane regions enriched in sphingolipids and sterols that have only recently, but intensively, been studied in plants. In this work, we report a detailed, easy-to-follow, and fast procedure to isolate detergent-resistant membranes (DRMs) from purified plasma membranes (PMs) that was used to obtain DRMs from Phaseolus vulgaris and Nicotiana tabacum leaves and germinating Zea mays embryos. Characterized according to yield, ultrastructure, and sterol composition, these DRM preparations showed similarities to analogous preparations from other eukaryotic cells. Isolation of DRMs from germinating maize embryos reveals the presence of microdomains at very early developmental stages of plants.
authors: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México DF 04510, Mexico.
authors: Laura
authors: L
authors: Carmona-Salazar
authors: Mohammed
authors: M
authors: El Hafidi
authors: Consuelo
authors: C
authors: Enríquez-Arredondo
authors: Christian
authors: C
authors: Vázquez-Vázquez
authors: Luis E
authors: LE
authors: González de la Vara
authors: Marina
authors: M
authors: Gavilanes-Ruíz
mesh_terms: D003902:Detergents / Q000737:chemistry; D021962:Membrane Microdomains / Q000737:chemistry* / Q000648:ultrastructure; D027805:Phaseolus / Q000737:chemistry* / Q000648:ultrastructure; D010788:Photosynthesis; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure; D013261:Sterols / Q000032:analysis / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry* / Q000648:ultrastructure; D003313:Zea mays / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D003902:Detergents; D013261:Sterols