title: Rapid determination of estrogens in milk samples based on magnetite nanoparticles/polypyrrole magnetic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry.
abstract: In this study, a nanocomposite of polypyrrole-coated magnetite nanoparticles (denoted as MNPs/PPy) was prepared and employed as magnetic solid-phase extraction (MSPE) sorbent for extraction of estrogens from milk samples. Because the polypyrrole coating possessed a highly π-conjugated structure and hydrophobicity, MNPs/PPy showed excellent performance for the estrogen extraction. Estrogens could be captured directly by MNPs/PPy from milk samples without protein precipitation. Moreover, the extraction could be carried out within 3 min. Thus, a rapid, simple, and effective method for the analysis of estrogens in milk samples was established by coupling MNPs/PPy-based MSPE with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The limits of detections for estrogens investigated were in the range of 5.1-66.7 ng/L. The recoveries of estrogens (concentration range of 0.5-20 ng/mL) from milk samples were in the range of 83.4-108.5%, with relative standard deviations ranging between 4.2 and 15.4%.
authors: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, People's Republic of China.
authors: Qiang
authors: Q
authors: Gao
authors: Dan
authors: D
authors: Luo
authors: Mei
authors: M
authors: Bai
authors: Zong-Wei
authors: ZW
authors: Chen
authors: Yu-Qi
authors: YQ
authors: Feng
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000032:analysis*; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles; D008892:Milk / Q000737:chemistry*; D011108:Polymers; D011758:Pyrroles; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D004967:Estrogens; D058185:Magnetite Nanoparticles; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole