title: Three-dimensional X-ray observation of atmospheric biological samples by linear-array scanning-electron generation X-ray microscope system.
abstract: Recently, we developed a soft X-ray microscope called the scanning-electron generation X-ray microscope (SGXM), which consists of a simple X-ray detection system that detects X-rays emitted from the interaction between a scanning electron beam (EB) and the thin film of the sample mount. We present herein a three-dimensional (3D) X-ray detection system that is based on the SGXM technology and designed for studying atmospheric biological samples. This 3D X-ray detection system contains a linear X-ray photodiode (PD) array. The specimens are placed under a CuZn-coated Si₃N₄ thin film, which is attached to an atmospheric sample holder. Multiple tilt X-ray images of the samples are detected simultaneously by the linear array of X-ray PDs, and the 3D structure is calculated by a new 3D reconstruction method that uses a simulated-annealing algorithm. The resulting 3D models clearly reveal the inner structure of the bacterium. In addition, the proposed method can easily be used for diverse samples in a broad range of scientific fields.
authors: Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Umezono, Tsukuba, Ibaraki, Japan. t-ogura@aist.go.jp
authors: Toshihiko
authors: T
authors: Ogura
mesh_terms: D000465:Algorithms; D001272:Atmosphere / Q000032:analysis*; D001419:Bacteria / Q000648:ultrastructure*; D003198:Computer Simulation; D004783:Environmental Microbiology; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation*; D008954:Models, Biological; D014965:X-Rays