title: Electrochemical immunosensor based on electron transfer mediated by graphene oxide initiated silver enhancement.
abstract: A new electrochemical immunosensor for the detection of protein biomarker platelet-derived growth factor BB (PDGF-BB) was developed based on graphene oxide (GO) initiated silver enhancement. The immunosensor was fabricated based on the traditional sandwich protocol using secondary anti-PDGF-BB antibody (Ab(2)) modified GO as label. Gold electrode was first modified with self-assembled monolayer (SAM) to block the electron transfer between the electrode and K(3)Fe(CN)(6) solution. After the immobilization of primary anti-PDGF-BB antibody (Ab(1)) onto electrode via aminidation to the carboxylic group of SAM and the formation of the sandwich immuno-structure onto electrode surface, the electrode was immersed into silver enhancement solution for silver deposition. The deposited metal silver onto GO then mediated electron transfer across the SAM, producing redox current. The resulting immunosensor displays a wide range of linear response, low detection limit, good reproducibility and stability. The immunosensor was used to the detection of PDGF-BB contents in serum samples with satisfactory results.
authors: Key Laboratory of Resources Chemistry of Nonferrous Metals, Ministry of Education, Central South University, Changsha 410083 China.
authors: Fengli
authors: F
authors: Qu
authors: Hongmei
authors: H
authors: Lu
authors: Minghui
authors: M
authors: Yang
authors: Chunyan
authors: C
authors: Deng
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000379:methods*; D001774:Blood Chemical Analysis; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000276:immunology; D020574:Proto-Oncogene Proteins c-sis; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D010087:Oxides; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D012834:Silver; D006108:Graphite