title: Rhododendrin, an analgesic/anti-inflammatory arylbutanoid glycoside, from the leaves of Rhododendron aureum.
abstract: To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Ericaceae), phytochemical isolation and pharmacological assays (writhing assays and vascular permeability assay for analgesic action in mice; carrageenan-induced paw edemaand TPA-induced ear edema assays of anti-inflammatory action in rats) were performed. Four compounds were isolated from the active fraction (BuOH fraction) by silica gel column chromatography and identified as (-)-rhododendrol, (-)-rhododendrin, avicularin and hyperoside by spectroscopic methods. Rhododendrin, the main compound of the BuOH fraction, exhibited significant analgesic actions in mice and anti-inflammatory actions in rats. This compound accounted for 3.1% of the MeOH extract and 0.48% of dried leaves, respectively, on HPLC analysis. These results suggest that rhododendrin is the major biologically active substance in the leaves of R. aureum with analgesic/anti-inflammatory activity.
authors: Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Korea.
authors: Myung-Hoe
authors: MH
authors: Kim
authors: Agung
authors: A
authors: Nugroho
authors: Jongwon
authors: J
authors: Choi
authors: Jong Hee
authors: JH
authors: Park
authors: Hee-Juhn
authors: HJ
authors: Park
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D006168:Guinea Pigs; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017207:Rats, Sprague-Dawley; D029793:Rhododendron / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D006027:Glycosides; D010936:Plant Extracts