title: [Intelukin-10 expression with immunorreglatory function in the mucosa of patients with ulcerative colitis].
abstract: BACKGROUND
  Interleukin-10 (IL-10) is an important immunoregulatory cytokine that acts on antigen presenting cells by the inhibiting both the synthesis of cytokines, co-stimulatory and HLA class II molecules.
  
  
  OBJECTIVE
  To study the gene and protein expression of IL-10 in the mucosa from patients with ulcerative colitis (UC).
  
  
  METHODS
  We studied 40 patients with UC and 18 controls without endoscopic evidence of intestinal inflammation. From rectal biopsies was determined the gene expression of IL- 10 by real time polymerase chain reaction (PCR). The detection of the protein in tissue was performed by immunohistochemistry.
  
  
  RESULTS
  patients with UC in remission had significantly higher expression of il-10 gene in mucosa compared to the group of patients with active UC (p = 0.01) and the control group (p = 0.05). All patients with active UC had pancolitis, while patients in remission from distal inflammation, 16 had extra-intestinal manifestations and 23 had mild to moderate inflammation with less than one relapse within a year. Patients with UC in remission had significantly higher expression of IL-10 gene in mucosa compared with the group of patients with active UC (p = 0.01) or the control group (p = 0.05).
  
  
  CONCLUSIONS
  The expression of IL-10 gene is increased in colonic mucosa from patients with UC in remission, confirming that it is an immunoregulatory cytokine that promotes remission in patients with UC.
authors: Clínica de Enfermedad Inflamatoria Intestinal, Departamento de Gastroenterología, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, México, D. F. gabrielafaster@gmail.com
authors: G
authors: G
authors: Fonseca-Camarillo
authors: J
authors: J
authors: Furuzawa-Carballeda
authors: B
authors: B
authors: Martínez-Benítez
authors: R
authors: R
authors: Barreto-Zúñiga
authors: K
authors: K
authors: Yamamoto-Furusho
mesh_terms: D000328:Adult; D001706:Biopsy; D003093:Colitis, Ulcerative / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000473:pathology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012007:Rectum / Q000378:metabolism / Q000473:pathology
chemical_list: D016753:Interleukin-10; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases