title: [Comparison of effect between vascularization osteogenesis and membrane guided osteogenesis in bone repair by tissue engineered bone with pedicled fascial flap packing autologous red bone marrow].
abstract: OBJECTIVE
  To compare the effect between vascularization osteogenesis and membrane guided osteogenesis in the bone repair by the tissue engineered bone with pedicled fascial flap packing autologous red bone marrow (ARBM), so as to provide a reference for the bone defect repair in clinic.
  
  
  METHODS
  The tissue engineered bone was constructed with ARBM and the osteoinductive absorbing recombinant human materials with recombinant human bone morphogenetic protein 2. Sixty New Zealand rabbits (aged 4-5 months, weighing 2.0-2.5 kg) were randomly divided into group A (n = 16), group B (n = 22), and group C (n = 22). The complete periosteum defect model of 1.5 cm in length was prepared in right ulnar bone, then the tissue engineered bone was implanted in the bone defect area in group A, the tissue engineered bone with free fascial flap in group B, and the tissue engineered bone with pedicled fascial flap in group C. At 4, 8, 12, and 16 weeks, the tissue of bone defect area was harvested from 4 rabbits of each group for the general, histological, and immunohistochemical staining observations; at 8, 12, and 16 weeks, 2 rabbits of groups B and C, respectively were selected to perform ink perfusion experiment by axillary artery.
  
  
  RESULTS
  The general observation showed that the periosteum-like tissues formed in the fascial flap of groups B and C, chondroid tissues formed in group B, new bone formed in group C, and the fibrous and connective tissues in group A at 4 and 8 weeks; a few porosis was seen in group A, more new bone in group B, and bone stump formation in group C at 12 and 16 weeks. Histological observation showed that there were few new blood vessels and new bone trabeculae in groups A and B, while there were large amounts of new blood vessels and mature bone trabeculae in group C at 4 and 8 weeks. There were a few new blood vessels and new bone trabeculae in group A; more blood vessels, significantly increased mature trabeculae, and the medullary cavity formation in group B; and gradually decreased blood vessels, the mature bone structure formation, and the re-opened medullary cavity in group C at 12 and 16 weeks. The immunohistochemical staining observation showed that the levels of CD105, CD34, and factor VIII were higher in group C than in groups A and B at different time points. The bone morphometry analysis showed that the trabecular volume increased gradually with time in 3 groups after operation; the trabecular volume in group C was significantly more than those in groups A and B at different time points (P < 0.05); and there was significant difference between groups A and B (P < 0.05) except the volume at 4 weeks (P > 0.05). The vascular image analysis showed that the vascular regenerative area ratio in group C was significantly higher than those in groups A and B at different time points (P < 0.05). The ink perfusion experiment showed that the osteogenic zone had sparse ink area with no obvious change in group B, while the osteogenic zone had more intensive ink area and reached the peak at 8 weeks, then decreased in group C.
  
  
  CONCLUSIONS
  The tissue engineered bone with pedicled fascial flap packing ARBM has the vascularization osteogenesis effect at early stage, but the effect disappears at late stage gradually when the membrane guided osteogenesis is main.
authors: Department of Orthopaedics, First Affiliated Hospital of Hebei North University, Zhangjiakou Hebei 075000, PR China. yxm1120@sohu.com
authors: Xinming
authors: X
authors: Yang
authors: Lei
authors: L
authors: Zhang
authors: Xianyong
authors: X
authors: Meng
authors: Yaoyi
authors: Y
authors: Wang
authors: Wei
authors: W
authors: Shi
authors: Yakun
authors: Y
authors: Du
authors: Zhenshun
authors: Z
authors: Hu
authors: Yanlin
authors: Y
authors: Yin
mesh_terms: D000818:Animals; D001853:Bone Marrow; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration; D018786:Bone Substitutes; D002478:Cells, Cultured; D005205:Fascia / Q000637:transplantation*; D005260:Female; D018919:Neovascularization, Physiologic; D010012:Osteogenesis; D011817:Rabbits; D013524:Surgical Flaps; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
chemical_list: D018786:Bone Substitutes