title: Platelet lysate mucohadesive formulation to treat oral mucositis in graft versus host disease patients: a new therapeutic approach.
abstract: Optimal treatment of oral mucositis (OM) due to graft versus host disease (GvHD) is currently not available. Platelet-derived growth factors (PDGFs) have high capability for tissue healing and may play a role in repairing the mucosal barrier. The aim of the present work was to develop a mucoadhesive formulation to administer platelet lysate to oral cavity prolonging contact time of platelet lysate with oral mucosa. The mucoadhesive formulation was characterized for in vitro properties (PDGF-AB concentration, mucoadhesive properties, cytotoxicity, fibroblast proliferation, wound healing). Moreover, a preliminary clinical study on seven GvHD patients with OM refractory to other therapies was conducted, to evaluate feasibility, safety, and efficacy. GVPL (mucoadhesive gel vehicle mixed with platelet lysate)showed good mucoadhesive properties; additionally, it was characterized by good biocompatibility in vitro on fibroblasts and it was able to enhance fibroblast proliferation and wound healing, maintaining the efficacy for up to 14 days following storage at 2-8°C. In vivo, clinical response was good-to-complete in five, fair in one, none in the remaining one. The in vitro results indicate that GVPL has optimal mucoadhesive and healing enhancer properties, maintained over time (up to 14 days); preliminary clinical results suggest that oral application of platelet lysate-loaded mucoadhesive formulation is feasible, safe, well tolerated, and effective. A larger controlled randomized study is needed.
authors: Immunohaematology and Transfusion Service, Apheresis And Cell Therapy Unit, Fondazione IRCCS Policlinico S. Matteo, Pavia, Italy.
authors: Claudia
authors: C
authors: Del Fante
authors: Cesare
authors: C
authors: Perotti
authors: Maria Cristina
authors: MC
authors: Bonferoni
authors: Silvia
authors: S
authors: Rossi
authors: Giuseppina
authors: G
authors: Sandri
authors: Franca
authors: F
authors: Ferrari
authors: Luigia
authors: L
authors: Scudeller
authors: Carla Marcella
authors: CM
authors: Caramella
mesh_terms: D000179:Acrylates / Q000737:chemistry; D000278:Administration, Buccal; D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002457:Cell Extracts / Q000737:chemistry; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts; D006086:Graft vs Host Disease / Q000150:complications; D006801:Humans; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage* / Q000627:therapeutic use; D013280:Stomatitis / Q000628:therapy*; D014014:Tissue Adhesives / Q000008:administration & dosage; D014783:Viscosity; D014945:Wound Healing
chemical_list: D000179:Acrylates; D002457:Cell Extracts; D010982:Platelet-Derived Growth Factor; D014014:Tissue Adhesives; C117167:carbopol 974P NF; C095477:platelet-derived growth factor AB