title: Phosphoprotein electrophoresis in the presence of Fe(III) ions.
abstract: Preparation of affinity polyacrylamide gels containing immobilized Fe(III) ions for the separation of proteins exhibiting metal ion binding properties is described. The presented method enables uniform distribution of immobilized metal ions in the affinity part of the polyacrylamide separating gel. Affinity gels prepared by this way are suitable to follow the effect of different concentrations of metal ions immobilized in polyacrylamide gel on a protein electrophoretic behavior. Polyacrylamide gels containing immobilized Fe(III) ions were used to study the electrophoretic behavior of two model proteins differing in their phosphate group content: chicken ovalbumin and bovine α-casein. For the electrophoretic separation, both the native and the denaturating conditions were used.
authors: Institute of Pathophysiology and CEH, 1st Faculty of Medicine, Charles University in Prague, Praha, Czech Republic. zkuce@lf1.cuni.cz.
authors: Zdenka
authors: Z
authors: Kučerová
authors: Hana
authors: H
authors: Muselová
authors: Petr
authors: P
authors: Přikryl
authors: Marie
authors: M
authors: Tichá
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D010750:Phosphoproteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005290:Ferric Compounds; D007477:Ions; D010750:Phosphoproteins