title: Label-free colorimetric and quantitative detection of cancer marker protein using noncrosslinking aggregation of Au/Ag nanoparticles induced by target-specific peptide probe.
abstract: Assays for non-enzyme protein based on peptide-protein interaction are few due to the fact that most of peptide-protein bindings do not produce easily measurable output signals. Here we report a homogenous assay for colorimetric and quantitative detection of a cancer marker and promising antitumor target, cyclin A(2), using noncrosslinking aggregation of unmodified AuNPs/AgNPs by utilizing the difference of coagulating ability of a cationic peptide probe (P1) and its binding form toward naked AuNPs/AgNPs. In the absence of cyclin A(2), P1 coagulates particles immediately, whereas cyclin A(2) binding prevents the interaction of P1 with metal particles surface, significantly reducing the magnitude of aggregation. The extent of aggregation is dependent on the concentration of the target protein cyclin A(2) and the difference in color can readily be distinguished by spectrometer and naked eyes. The assay is sensitive and selective. Cyclin A(2) assay using AuNPs as colorimetric indicator is more easily monitored by naked eyes owing to the distinct color change, and 40 nM cyclin A(2) can be detected without the aid of any instruments. Using inexpensive desktop spectrometer, cyclin A(2) assay using AgNPs as colorimetric indicator can detect as low as 30 nM cyclin A(2), which is 20 fold lower than that of cyclin A(2) assay using terbium-chelating peptide as the probe reported recently (Pazos et al., 2008, 130, 9652-9653). This strategy will shed light on developing of unlabeled peptide-based protein biosensors.
authors: Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors: Xiaohui
authors: X
authors: Wang
authors: Li
authors: L
authors: Wu
authors: Jinsong
authors: J
authors: Ren
authors: Daisuke
authors: D
authors: Miyoshi
authors: Naoki
authors: N
authors: Sugimoto
authors: Xiaogang
authors: X
authors: Qu
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000191:economics / Q000379:methods*; D045744:Cell Line, Tumor; D003124:Colorimetry / Q000191:economics / Q000379:methods; D056751:Cyclin A2 / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; C503721:CCNA2 protein, human; D056751:Cyclin A2; D010455:Peptides; D012834:Silver; D006046:Gold