title: Stomatin-like protein 2 binds cardiolipin and regulates mitochondrial biogenesis and function.
abstract: Stomatin-like protein 2 (SLP-2) is a widely expressed mitochondrial inner membrane protein of unknown function. Here we show that human SLP-2 interacts with prohibitin-1 and -2 and binds to the mitochondrial membrane phospholipid cardiolipin. Upregulation of SLP-2 expression increases cardiolipin content and the formation of metabolically active mitochondrial membranes and induces mitochondrial biogenesis. In human T lymphocytes, these events correlate with increased complex I and II activities, increased intracellular ATP stores, and increased resistance to apoptosis through the intrinsic pathway, ultimately enhancing cellular responses. We propose that the function of SLP-2 is to recruit prohibitins to cardiolipin to form cardiolipin-enriched microdomains in which electron transport complexes are optimally assembled. Likely through the prohibitin functional interactome, SLP-2 then regulates mitochondrial biogenesis and function.
authors: The Centre for Human Immunology, Robarts Research Institute, Department of Microbiology and Immunology, and Department of Medicine, The University of Western Ontario, London, Ontario, Canada.
authors: Darah A
authors: DA
authors: Christie
authors: Caitlin D
authors: CD
authors: Lemke
authors: Isaac M
authors: IM
authors: Elias
authors: Luan A
authors: LA
authors: Chau
authors: Mark G
authors: MG
authors: Kirchhof
authors: Bo
authors: B
authors: Li
authors: Eric H
authors: EH
authors: Ball
authors: Stanley D
authors: SD
authors: Dunn
authors: Grant M
authors: GM
authors: Hatch
authors: Joaquín
authors: J
authors: Madrenas
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D017209:Apoptosis; D001798:Blood Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D002308:Cardiolipins / Q000378:metabolism*; D004579:Electron Transport; D006801:Humans; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D000091402:Prohibitins; D034622:RNA Interference; D034741:RNA, Small Interfering; D012097:Repressor Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D002308:Cardiolipins; D008565:Membrane Proteins; D000091402:Prohibitins; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C406287:STOML2 protein, human; D000255:Adenosine Triphosphate