title: Antibacterial mesh: a novel technique involving naturally occurring cellular proteins.
abstract: BACKGROUND
  Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh.
  
  
  METHODS
  Lysozyme, human beta defensin (HBD-3), human cathelicidin (LL-37), and lysostaphin were adsorbed to polypropylene mesh at various concentrations. Treated meshes were placed in a suspension of 1 × 10(6) Staphylococcus aureus. Antibacterial action was monitored by turbidimetric assay, fluorescent imaging, and a colony counting method.
  
  
  RESULTS
  A very high rate of lysis of S aureus cells was observed in the lysostaphin-treated group as measured by optical density; none survived as seen on colony count assays. Optical density for mesh coated with lysozyme, HBD-3, and LL-37 did not differ from untreated controls, with 100% survival rates by colony counts.
  
  
  CONCLUSIONS
  Lysostaphin had superior antibacterial activity following adsorption to mesh.
authors: Carolinas Medical Center, Charlotte, NC 28204, USA.
authors: Yuliya
authors: Y
authors: Yurko
authors: Kathleen
authors: K
authors: McDeavitt
authors: Rohan Satish
authors: RS
authors: Kumar
authors: Terri
authors: T
authors: Martin
authors: Ajita
authors: A
authors: Prabhu
authors: Amy E
authors: AE
authors: Lincourt
authors: Alexey
authors: A
authors: Vertegel
authors: B Todd
authors: BT
authors: Heniford
mesh_terms: D000327:Adsorption; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D015169:Colony Count, Microbial; D006801:Humans; D008248:Lysostaphin / Q000494:pharmacology*; D051379:Mice; D009113:Muramidase / Q000494:pharmacology*; D011126:Polypropylenes; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000187:drug effects; D013526:Surgical Mesh / Q000382:microbiology*; D013530:Surgical Wound Infection / Q000517:prevention & control*; D023083:beta-Defensins / Q000494:pharmacology*; D054804:Cathelicidins
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011126:Polypropylenes; D023083:beta-Defensins; D009113:Muramidase; D008248:Lysostaphin; D054804:Cathelicidins