title: STENOFOLIA regulates blade outgrowth and leaf vascular patterning in Medicago truncatula and Nicotiana sylvestris.
abstract: Dicot leaf primordia initiate at the flanks of the shoot apical meristem and extend laterally by cell division and cell expansion to form the flat lamina, but the molecular mechanism of lamina outgrowth remains unclear. Here, we report the identification of STENOFOLIA (STF), a WUSCHEL-like homeobox transcriptional regulator, in Medicago truncatula, which is required for blade outgrowth and leaf vascular patterning. STF belongs to the MAEWEST clade and its inactivation by the transposable element of Nicotiana tabacum cell type1 (Tnt1) retrotransposon insertion leads to abortion of blade expansion in the mediolateral axis and disruption of vein patterning. We also show that the classical lam1 mutant of Nicotiana sylvestris, which is blocked in lamina formation and stem elongation, is caused by deletion of the STF ortholog. STF is expressed at the adaxial-abaxial boundary layer of leaf primordia and governs organization and outgrowth of lamina, conferring morphogenetic competence. STF does not affect formation of lateral leaflets but is critical to their ability to generate a leaf blade. Our data suggest that STF functions by modulating phytohormone homeostasis and crosstalk directly linked to sugar metabolism, highlighting the importance of coordinating metabolic and developmental signals for leaf elaboration.
authors: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, USA. million.tadege@okstate.edu
authors: Million
authors: M
authors: Tadege
authors: Hao
authors: H
authors: Lin
authors: Mohamed
authors: M
authors: Bedair
authors: Ana
authors: A
authors: Berbel
authors: Jiangqi
authors: J
authors: Wen
authors: Clemencia M
authors: CM
authors: Rojas
authors: Lifang
authors: L
authors: Niu
authors: Yuhong
authors: Y
authors: Tang
authors: Lloyd
authors: L
authors: Sumner
authors: Pascal
authors: P
authors: Ratet
authors: Neil A
authors: NA
authors: McHale
authors: Francisco
authors: F
authors: Madueño
authors: Kirankumar S
authors: KS
authors: Mysore
mesh_terms: D035264:Flowers / Q000033:anatomy & histology / Q000254:growth & development; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism; D046913:Medicago truncatula / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D010641:Phenotype; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D018626:Retroelements; D014026:Nicotiana / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018626:Retroelements