title: Rb1 protects endothelial cells from hydrogen peroxide-induced cell senescence by modulating redox status.
abstract: Senescence of endothelial cells has been proposed to play an important role in endothelial dysfunction and atherogenesis. In the present study we aimed to investigate whether ginsenoside Rb1, a major constituent of ginseng, protects endothelial cells from H(2)O(2)-induced endothelial senescence. While H(2)O(2) induced premature senescent-like phenotype of human umbilical vein endothelial cells (HUVECs), as judged by increased senescence-associated β-galactosidase (SA-β-gal) activity, enlarged, flattened cell morphology and sustained growth arrest, our results demonstrated that Rb1 protected endothelial cells from oxidative stress induced senescence. Mechanistically, we found that Rb1 could markedly increase intracellular superoxide dismutase (Cu/Zn SOD/SOD1) activity and decrease the malondialdehyde (MDA) level in H(2)O(2)-treated HUVECs, and suppress the generation of intracellular reactive oxygen species (ROS). Consistent with these findings, Rb1 could effectively restore the protein expression of Cu/Zn SOD, which was down-regulated in H(2)O(2) treated cells. Taken together, our data demonstrate that Rb1 exhibits antioxidant effects and antagonizes H(2)O(2)-induced cellular senescence.
authors: Department of Cardiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors: Ding-Hui
authors: DH
authors: Liu
authors: Yan-Ming
authors: YM
authors: Chen
authors: Yong
authors: Y
authors: Liu
authors: Bao-Shun
authors: BS
authors: Hao
authors: Bin
authors: B
authors: Zhou
authors: Lin
authors: L
authors: Wu
authors: Min
authors: M
authors: Wang
authors: Lin
authors: L
authors: Chen
authors: Wei-Kang
authors: WK
authors: Wu
authors: Xiao-Xian
authors: XX
authors: Qian
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D016166:Free Radical Scavengers; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D060888:Real-Time Polymerase Chain Reaction; D016160:Retinoblastoma Protein / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D016166:Free Radical Scavengers; D016160:Retinoblastoma Protein; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase