title: Diversity of anti-haemostatic proteins in the salivary glands of Rhodnius species transmitters of Chagas disease in the greater Amazon.
abstract: The triatomines in the tribe Rhodniini are the main vectors of the Trypanosoma cruzi to humans in recent outbreaks of acute Chagas disease in the Amazon. These insects dwelling in palm trees do not colonize the human domicile. Their success to transmit the infection relies partially on the efficacy of their salivary gland apparatuses. Here we show the transcriptome of the Rhodnius brethesi and Rhodnius robustus salivary glands, comprising 56 and 122 clusters, respectively. Approximately one third of these clusters are described for the first time. The LC-MS/MS analysis identified 123 and 111 proteins in R. brethesi and R. robustus sialome, respectively. Noteworthy, lipocalin platelet aggregation inhibitors, inositol polyphosphate 5-phosphatases, and Kazal domain proteins, which are essential for the insect's successful acquisition of blood meals, were found in our analysis. Moreover, glutathione S transferase and antigen-5, which play roles in the insect's defense and resistance against insecticide, were also observed.
authors: Chagas Disease Multidisciplinary Research Laboratory, Faculty of Medicine, University of Brasília, Federal District, Brazil.
authors: Ana C M
authors: AC
authors: Bussacos
authors: Ernesto S
authors: ES
authors: Nakayasu
authors: Mariana M
authors: MM
authors: Hecht
authors: Juliana A
authors: JA
authors: Parente
authors: Célia M A
authors: CM
authors: Soares
authors: Antônio R L
authors: AR
authors: Teixeira
authors: Igor C
authors: IC
authors: Almeida
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology / Q000635:transmission; D020869:Gene Expression Profiling; D005982:Glutathione Transferase / Q000032:analysis / Q000235:genetics; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D019476:Insect Proteins / Q000032:analysis; D007303:Insect Vectors / Q000737:chemistry* / Q000472:pathogenicity; D007305:Insecticide Resistance / Q000235:genetics; D054834:Lipocalins / Q000032:analysis / Q000235:genetics; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000235:genetics; D010975:Platelet Aggregation Inhibitors / Q000032:analysis; D012239:Rhodnius / Q000737:chemistry* / Q000469:parasitology; D012469:Salivary Glands / Q000737:chemistry* / Q000469:parasitology; D014862:Wasp Venoms / Q000032:analysis / Q000235:genetics
chemical_list: D019476:Insect Proteins; D054834:Lipocalins; D010975:Platelet Aggregation Inhibitors; D014862:Wasp Venoms; C040583:white-faced hornet venom antigen 5; D005982:Glutathione Transferase; D010744:Phosphoric Monoester Hydrolases; D000072181:Inositol Polyphosphate 5-Phosphatases