title: rAAV-mediated delivery of brain-derived neurotrophic factor promotes neurite outgrowth and protects neurodegeneration in focal ischemic model.
abstract: Stroke is one of the neurological diseases which lead to permanently neuronal damage after temporary or long-term occlusion of vessels or after heart attack. However, there are few efficient strategies to prevent or treat this kind of insult in clinical because the consequence is irreversible and could be long-lasting after the onset of stroke. Gene therapy especially using viral system has long been addressed to be of great potential to reduce the damage. Here, we generated recombinant adeno-associated virus (rAAV) carrying brain-derived neurotrophic factor (BDNF) gene. Cells infected with rAAV-BDNF could be able to produce functional BDNF which promoted neurite outgrowth and protected neurons from apoptosis induced by serum deprivation. Further more, single injection of rAAV showed neuroprotection against cell death in focal ischemic model. These results showed that rAAV-mediated gene delivery is functional, which shed light to the future application of viral system-based gene therapy in clinical.
authors: Department of Neurology, The fourth hospital of Harbin medical university, Heilongjiang, 150001,China.
authors: Jingyu
authors: J
authors: Zhang
authors: Zhigang
authors: Z
authors: Yu
authors: Zhiqiang
authors: Z
authors: Yu
authors: Zichao
authors: Z
authors: Yang
authors: Hong
authors: H
authors: Zhao
authors: Luran
authors: L
authors: Liu
authors: Jiexu
authors: J
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D002545:Brain Ischemia / Q000473:pathology / Q000628:therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000008:administration & dosage* / Q000235:genetics / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000235:genetics; D000229:Dependovirus / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D009410:Nerve Degeneration / Q000473:pathology / Q000517:prevention & control*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019076:Transgenes / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D018076:DNA, Complementary; D018696:Neuroprotective Agents