title: Methionine and homocysteine modulate the rate of ROS generation of isolated mitochondria in vitro.
abstract: Dietary methionine restriction and supplementation in mammals have beneficial (antiaging) and detrimental effects respectively, which have been related to chronic modifications in the rate of mitochondrial ROS generation. However it is not known if methionine or its metabolites can have, in addition, direct effects on the rate of mitochondrial ROS production. This is studied here for the methionine cycle metabolites S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), homocysteine and methionine itself in isolated rat liver, kidney, heart, and brain mitochondria. The results show that methionine increases ROS production in liver and kidney mitochondria, homocysteine increases it in kidney and decreases it in the other three organs, and SAM and SAH have no effects. The variations in ROS production are localized at complexes I or III. These changes add to previously described chronic effects of methionine restriction and supplementation in vivo.
authors: Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain.
authors: Jose
authors: J
authors: Gomez
authors: Ines
authors: I
authors: Sanchez-Roman
authors: Alexia
authors: A
authors: Gomez
authors: Carlota
authors: C
authors: Sanchez
authors: Henar
authors: H
authors: Suarez
authors: Monica
authors: M
authors: Lopez-Torres
authors: Gustavo
authors: G
authors: Barja
mesh_terms: D000818:Animals; D006710:Homocysteine / Q000378:metabolism* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D006710:Homocysteine; D008715:Methionine; D006861:Hydrogen Peroxide