title: Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids.
abstract: Uniparental chromosome elimination occurs in several interspecific hybrids of plants. We studied the mechanism underlying selective elimination of the paternal chromosomes during the early development of Hordeum vulgare × Hordeum bulbosum embryos. The following conclusions regarding the role of the centromere-specific histone H3 variant (CENH3) in the process of chromosome elimination were drawn: (i) centromere inactivity of H. bulbosum chromosomes triggers the mitosis-dependent process of uniparental chromosome elimination in unstable H. vulgare × H. bulbosum hybrids; (ii) centromeric loss of CENH3 protein rather than uniparental silencing of CENH3 genes causes centromere inactivity; (iii) in stable species combinations, cross-species incorporation of CENH3 occurs despite centromere-sequence differences, and not all CENH3 variants get incorporated into centromeres if multiple CENH3s are present in species combinations; and (iv) diploid barley species encode two CENH3 variants, the proteins of which are intermingled within centromeres throughout mitosis and meiosis.
authors: Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany.
authors: Maryam
authors: M
authors: Sanei
authors: Richard
authors: R
authors: Pickering
authors: Katrin
authors: K
authors: Kumke
authors: Shuhei
authors: S
authors: Nasuda
authors: Andreas
authors: A
authors: Houben
mesh_terms: D002503:Centromere / Q000737:chemistry*; D002678:Chimera; D032461:Chromosomes, Plant; D006657:Histones / Q000378:metabolism*; D001467:Hordeum / Q000235:genetics*; D008540:Meiosis; D008938:Mitosis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D024182:Uniparental Disomy
chemical_list: D006657:Histones; D010940:Plant Proteins