title: Use of volatile compound metabolic signatures in poultry liver to back-trace dietary exposure to rapidly metabolized xenobiotics.
abstract: The study investigated the feasibility of using volatile compound signatures of liver tissues in poultry to detect previous dietary exposure to different types of xenobiotic. Six groups of broiler chickens were fed a similar diet either noncontaminated or contaminated with polychlorinated dibenzo-p-dioxins/-furans (PCDD/Fs; 3.14 pg WHO-TEQ/g feed, 12% moisture), polychlorinated biphenyls (PCBs; 0.08 pg WHO-TEQ/g feed, 12% moisture), polybrominated diphenyl ethers (PBDEs; 1.63 ng/g feed, 12% moisture), polycyclic aromatic hydrocarbons (PAHs; 0.72 μg/g fresh matter), or coccidiostats (0.5 mg/g feed, fresh matter). Each chicken liver was analyzed by solid-phase microextraction - mass spectrometry (SPME-MS) for volatile compound metabolic signature and by gas chromatography - high resolution mass spectrometry (GC-HRMS), gas chromatography - tandem mass spectrometry (GC-MS/MS), and liquid chromatography - tandem mass spectrometry (LC-MS/MS) to quantify xenobiotic residues. Volatile compound signature evidenced a liver metabolic response to PAH although these rapidly metabolized xenobiotics are undetectable in this organ by the reference methods. Similarly, the volatile compound metabolic signature enabled to differentiate the noncontaminated chickens from those contaminated with PBDEs or coccidiostats. In contrast, no clear signature was pointed out for slowly metabolized compounds such as PCDD/Fs and PCBs although their residues were found in liver at 50.93 (±6.71) and 0.67 (±0.1) pg WHO-TEQ/g fat, respectively.
authors: INRA, UR370 QuaPA, MASS Team, Saint-Genès-Champanelle, France.
authors: Philippe
authors: P
authors: Berge
authors: Jérémy
authors: J
authors: Ratel
authors: Agnès
authors: A
authors: Fournier
authors: Catherine
authors: C
authors: Jondreville
authors: Cyril
authors: C
authors: Feidt
authors: Brigitte
authors: B
authors: Roudaut
authors: Bruno
authors: B
authors: Le Bizec
authors: Erwan
authors: E
authors: Engel
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism; D003049:Coccidiostats / Q000378:metabolism; D004032:Diet; D004781:Environmental Exposure / Q000032:analysis*; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D055442:Metabolome; D011078:Polychlorinated Biphenyls / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011200:Poultry / Q000378:metabolism*; D014835:Volatilization; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D003049:Coccidiostats; D055768:Halogenated Diphenyl Ethers; D000072317:Polychlorinated Dibenzodioxins; D011084:Polycyclic Aromatic Hydrocarbons; D015262:Xenobiotics; D011078:Polychlorinated Biphenyls