title: [The development of a test-system for the quantitative and qualitative evaluation of DNA content in criminalistic objects by the real-time polymerase chain reaction].
abstract: An original test-system for the preliminary quantitative and qualitative evaluation of isolated DNA is proposed by the polymerase chain reaction in real time (PCR-RT) based on the TaqMan technology. This test-system permits to simultaneously measure the amount of DNA in the sample, identify the genetic gender, and detect PCR inhibitors. The method has been approbated in the practical work of forensic medical experts.
authors: M I
authors: MI
authors: Lapenkov
authors: N V
authors: NV
authors: Plakhina
authors: Ia I
authors: IaI
authors: Alekseev
authors: D A
authors: DA
authors: Varlamov
mesh_terms: D000818:Animals; D003415:Crime / Q000331:legislation & jurisprudence; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D053803:Forensic Genetics / Q000331:legislation & jurisprudence / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling
chemical_list: D007202:Indicators and Reagents; D004247:DNA