title: A polysaccharide from Strongylocentrotus nudus eggs protects against myelosuppression and immunosuppression in cyclophosphamide-treated mice.
abstract: To assess the chemoprotective properties of a polysaccharide from Strongylocentrotus nudus eggs (SEP), myelosuppressed and immunosuppressed mouse models were generated by administration of cyclophosphamide (Cy) and then treated with SEP. SEP (16 mg/kg/d) remarkably increased spleen and thymus indices, activated the proliferation of leukocytes and erythrocytes and platelets from peripheral blood, and exhibited co-mitogenic activity on ConA- or LPS-stimulated splenocytes in a dose-dependent manner. An increased percentage of CD34(+) cells in bone marrow of Cy-treated mice was also observed. Furthermore, SEP elevated CD4(+) T lymphocyte counts as well as the CD4/CD8 ratio dose-dependently, and it increased interleukin-2 (IL-2), IgA, IgM, and IgG levels in the sera of Cy-treated mice. Pre-incubation with TLR2 and TLR4 blocking antibodies inhibited splenocyte proliferation and its IL-2 secretion. Finally, SEP significantly induced Akt phosphorylation in splenocytes from Cy-treated mice, suggesting that chemoprotection by SEP was mediated through the PI3K/Akt signaling pathway. These findings indicate that SEP plays an important role in the protection against myelosuppression and immunosuppression in Cy-treated mice and could be a potential immunomodulatory agent.
authors: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors: Hui
authors: H
authors: Wang
authors: Mengyu
authors: M
authors: Wang
authors: Jing
authors: J
authors: Chen
authors: Ying
authors: Y
authors: Tang
authors: Jie
authors: J
authors: Dou
authors: Jian
authors: J
authors: Yu
authors: Tao
authors: T
authors: Xi
authors: Changlin
authors: C
authors: Zhou
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D007108:Immune Tolerance / Q000187:drug effects*; D007136:Immunoglobulins / Q000097:blood; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000737:chemistry* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D047269:Strongylocentrotus / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D007136:Immunoglobulins; D007376:Interleukin-2; D011134:Polysaccharides; D003520:Cyclophosphamide