title: A modified immunoblot method to identify substrates of protein kinases.
abstract: While protein kinases are key components in multiple cellular processes, efficient identification of cognate in vivo substrates remains challenging. Here we describe a powerful method to screen potential substrates of protein kinases by partial transfer of proteins from a 2D-PAGE gel to a Western blot membrane. This approach allowed precise pinpointing of candidate substrate spots in the 2D gel, and identifying physiological substrates of protein kinases in Mycobacterium tuberculosis.
authors: Department of Biological Science, Wayne State University, 5047 Gullen Mall, Detroit, MI 48202, USA. cmkang1@biology.biosci.wayne.edu
authors: Choong-Min
authors: CM
authors: Kang
authors: Wan Jin
authors: WJ
authors: Jahng
authors: Robert N
authors: RN
authors: Husson
authors: Sang Hee
authors: SH
authors: Lee
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011494:Protein Kinases