title: Expression of phosphoinositide-specific phospholipase C isoforms in human umbilical vein endothelial cells.
abstract: OBJECTIVE
  The signalling system of phosphoinositides (PIs) is involved in a number of cell and tissue functions including membrane trafficking, ion channel activity, cell cycle, apoptosis, differentiation and cell and tissue polarity. Recently, a role in cell migration was hypothesised for PI and related molecules including the phosphoinositide-specific phospholipases C (PI-PLCs), main players in PI signalling. The expression of PI-PLCs is tissue-specific and evidence suggests that it varies under different conditions such as tumour progression or cell activation. In order to obtain a complete picture, the expression of all PI-PLC isoforms was analysed in human endothelial cells (EC).
  
  
  METHODS
  Using molecular biology methods (RT-PCR), the expression of PI-PLC isoforms was analysed in human umbilical vein endothelial cells (HUVEC), a widely used experimental model for human EC.
  
  
  RESULTS
  All the PI-PLC isoforms except PI-PLC β1, PI-PLC ε and PI-PLC ζ were expressed in HUVEC.
  
  
  CONCLUSIONS
  The growing interest in the complex cascade of events occurring in angiogenesis will provide useful insights for therapeutic strategies. The expression of PI-PLC isoforms in HUVEC is a useful tool for further studies directed to understanding their role in angiogenesis. However, although HUVEC represent a widely used experimental model for human macrovascular EC, limitations remain in that they cannot fully represent the metabolic properties and interactions of the EC distributed in the entire organism.
authors: Organi di Senso Department, Sapienza University of Rome, Rome, Italy.
authors: V R
authors: VR
authors: Lo Vasco
authors: L
authors: L
authors: Pacini
authors: T
authors: T
authors: Di Raimo
authors: D
authors: D
authors: D'arcangelo
authors: R
authors: R
authors: Businaro
mesh_terms: D002478:Cells, Cultured; D042783:Endothelial Cells / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes; D054801:Phosphoinositide Phospholipase C / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D054801:Phosphoinositide Phospholipase C