title: Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly.
abstract: Histone H3 variant H3.3, while differing from canonical H3 (H3.1) by only five amino acids, is assembled into nucleosomes, along with histone H4, at genic regions by the histone chaperone HIRA, whereas H3.1 is assembled into nucleosomes in a CAF-1-dependent reaction. Here, we show that phosphorylation of histone H4 Ser 47 (H4S47ph), catalyzed by the PAK2 kinase, promotes nucleosome assembly of H3.3-H4 and inhibits nucleosome assembly of H3.1-H4 by increasing the binding affinity of HIRA to H3.3-H4 and reducing association of CAF-1 with H3.1-H4. These results reveal a mechanism whereby H4S47ph distinctly regulates nucleosome assembly of H3.1 and H3.3.
authors: Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
authors: Bin
authors: B
authors: Kang
authors: Mintie
authors: M
authors: Pu
authors: Gangqing
authors: G
authors: Hu
authors: Weihong
authors: W
authors: Wen
authors: Zigang
authors: Z
authors: Dong
authors: Keji
authors: K
authors: Zhao
authors: Bruce
authors: B
authors: Stillman
authors: Zhiguo
authors: Z
authors: Zhang
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D056488:Histone Chaperones / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D018832:Molecular Chaperones; D009707:Nucleosomes / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D012694:Serine / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D054462:p21-Activated Kinases
chemical_list: C491641:ASF1A protein, human; C491640:ASF1B protein, human; C489780:CNOT8 protein, human; D018797:Cell Cycle Proteins; D002843:Chromatin; C086057:HIRA protein, human; D056488:Histone Chaperones; D006657:Histones; D018832:Molecular Chaperones; D009707:Nucleosomes; D014157:Transcription Factors; D012694:Serine; C515907:PAK2 protein, human; D054462:p21-Activated Kinases