title: Measurement of glycidol hemoglobin adducts in humans who ingest edible oil containing small amounts of glycidol fatty acid esters.
abstract: Hemoglobin (Hb) adducts are frequently used to address and/or monitor exposure to reactive chemicals. Glycidol (G), a known animal carcinogen, has been reported to form Hb adducts. Here, we measure G adduct levels in humans who daily ingest DAG oil, an edible oil consisting mainly of diacylglycerol. Since DAG oil contains a small amount of glycidol fatty acid esters (GEs), possible exposure to G released from GEs has been raised as a possible concern. For measurement of Hb adducts, we employed the N-alkyl Edman method reported by Landin et al. (1996) using gas chromatography-tandem mass spectrometry with minor modifications to detect G-Hb adducts as N-(2,3-dihydroxy-propyl)valine (diHOPrVal). Blood samples were collected from 7 DAG oil users and 6 non-users, and then G-Hb adduct levels were measured. G-Hb adducts were detected in all samples. The average level of diHOPrVal was 3.5±1.9pmol/g globin in the DAG oil users and 7.1±3.1pmol/g globin in the non-users. We conclude that there is no increased exposure to G in individuals who daily ingest DAG oil.
authors: Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan.
authors: Hiroshi
authors: H
authors: Honda
authors: Masayuki
authors: M
authors: Onishi
authors: Kenkichi
authors: K
authors: Fujii
authors: Naohiro
authors: N
authors: Ikeda
authors: Tohru
authors: T
authors: Yamaguchi
authors: Taketoshi
authors: T
authors: Fujimori
authors: Naohiro
authors: N
authors: Nishiyama
authors: Toshio
authors: T
authors: Kasamatsu
mesh_terms: D000328:Adult; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism; D004852:Epoxy Compounds / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020005:Propanols / Q000097:blood*; D053719:Tandem Mass Spectrometry; D014633:Valine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D004042:Dietary Fats, Unsaturated; D004852:Epoxy Compounds; D006454:Hemoglobins; C416044:N-(2,3-dihydroxypropyl)valine; D020005:Propanols; D014633:Valine; C004312:glycidol