title: Licocalchone-C extracted from Glycyrrhiza glabra inhibits lipopolysaccharide-interferon-γ inflammation by improving antioxidant conditions and regulating inducible nitric oxide synthase expression.
abstract: The genus Glycyrrhiza consists of about 30 species, amoung these, G. glabra is the source of several phenolic compounds, known as flavonoids, such as licoagrodin, licoagrochalcones, licoagroaurone and licochalcone C, kanzonol Y, glyinflanin B and glycyrdione A, which have shown various pharmacological activities, including antitumor, antiparasitic, antileishmanial, anti-ulcer and antioxidative effects. Among these compounds, licochalcone C was isolated but its biology has not been fully examined. In our study we reproduced an inflammatory state by treating THP-1 (human myelomonocytic leukaemia) cells with pro-inflammatory stimuli, such as LPS and IFN-γ and we investigated the possible antioxidant activity of licochalcone C at a concentration of 50 μM. Our results show that treatment with licochalcone C attenuates the LPS-IFN-γ-induced inflammatory response by significantly decreasing the expression and activity of iNOS via NFκB (nuclear factor kappa-B), by influencing extracellular O₂⁻ production, and by modulating the antioxidant network activity of SOD (superoxide dismutase), CAT (catalase) and GPx (glutathione peroxidase) activity. Based on these results we hypothesize that Licochalcone C has antioxidant properties since it reduces the production of superoxide radicals and consequently reduces the activity of iNOS.
authors: Department of Human Movement Sciences, University G. D'Annunzio of Chieti, 66123, Italy. s.franceschelli@unich.it
authors: Sara
authors: S
authors: Franceschelli
authors: Mirko
authors: M
authors: Pesce
authors: Isabella
authors: I
authors: Vinciguerra
authors: Alessio
authors: A
authors: Ferrone
authors: Graziano
authors: G
authors: Riccioni
authors: Antonia
authors: A
authors: Patruno
authors: Alfredo
authors: A
authors: Grilli
authors: Mario
authors: M
authors: Felaco
authors: Lorenza
authors: L
authors: Speranza
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D045744:Cell Line, Tumor; D047188:Chalcones / Q000302:isolation & purification* / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D047188:Chalcones; D008070:Lipopolysaccharides; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II