title: Immunoglobulin G locus events in soft tissue sarcoma cell lines.
abstract: Recently immunoglobulins (Igs) have been found to be expressed by cells other than B lymphocytes, including various human carcinoma cells. Sarcomas are derived from mesenchyme, and the knowledge about the occurrence of Ig production in sarcoma cells is very limited. Here we investigated the phenomenon of immunoglobulin G (IgG) expression and its molecular basis in 3 sarcoma cell lines. The mRNA transcripts of IgG heavy chain and kappa light chain were detected by RT-PCR. In addition, the expression of IgG proteins was confirmed by Western blot and immunofluorescence. Immuno-electron microscopy localized IgG to the cell membrane and rough endoplasmic reticulum. The essential enzymes required for gene rearrangement and class switch recombination, and IgG germ-line transcripts were also identified in these sarcoma cells. Chromatin immunoprecipitation results demonstrated histone H3 acetylation of both the recombination activating gene and Ig heavy chain regulatory elements. Collectively, these results confirmed IgG expression in sarcoma cells, the mechanism of which is very similar to that regulating IgG expression in B lymphocytes.
authors: Department of Pathology, Shantou University Medical College, Shantou, China.
authors: Zhengshan
authors: Z
authors: Chen
authors: Jing
authors: J
authors: Li
authors: Yanna
authors: Y
authors: Xiao
authors: Junjun
authors: J
authors: Zhang
authors: Yingying
authors: Y
authors: Zhao
authors: Yuxuan
authors: Y
authors: Liu
authors: Changchun
authors: C
authors: Ma
authors: Yamei
authors: Y
authors: Qiu
authors: Jin
authors: J
authors: Luo
authors: Guowei
authors: G
authors: Huang
authors: Christine
authors: C
authors: Korteweg
authors: Jiang
authors: J
authors: Gu
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D056426:Genetic Loci / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012509:Sarcoma / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D050441:VDJ Exons / Q000235:genetics
chemical_list: D006657:Histones; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D009363:Neoplasm Proteins; D012333:RNA, Messenger