title: Detection of undistorted continuous wave (CW) electron paramagnetic resonance (EPR) spectra with non-adiabatic rapid sweep (NARS) of the magnetic field.
abstract: A continuous wave (CW) electron paramagnetic resonance (EPR) spectrum is typically displayed as the first harmonic response to the application of 100 kHz magnetic field modulation, which is used to enhance sensitivity by reducing the level of 1/f noise. However, magnetic field modulation of any amplitude causes spectral broadening and sacrifices EPR spectral intensity by at least a factor of two. In the work presented here, a CW rapid-scan spectroscopic technique that avoids these compromises and also provides a means of avoiding 1/f noise is developed. This technique, termed non-adiabatic rapid sweep (NARS) EPR, consists of repetitively sweeping the polarizing magnetic field in a linear manner over a spectral fragment with a small coil at a repetition rate that is sufficiently high that receiver noise, microwave phase noise, and environmental microphonics, each of which has 1/f characteristics, are overcome. Nevertheless, the rate of sweep is sufficiently slow that adiabatic responses are avoided and the spin system is always close to thermal equilibrium. The repetitively acquired spectra from the spectral fragment are averaged. Under these conditions, undistorted pure absorption spectra are obtained without broadening or loss of signal intensity. A digital filter such as a moving average is applied to remove high frequency noise, which is approximately equivalent in bandwidth to use of an integrating time constant in conventional field modulation with lock-in detection. Nitroxide spectra at L- and X-band are presented.
authors: National Biomedical EPR Center, Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
authors: Aaron W
authors: AW
authors: Kittell
authors: Theodore G
authors: TG
authors: Camenisch
authors: Joseph J
authors: JJ
authors: Ratke
authors: Jason W
authors: JW
authors: Sidabras
authors: James S
authors: JS
authors: Hyde
mesh_terms: D000465:Algorithms; D003497:Cyclic N-Oxides / Q000737:chemistry; D003627:Data Interpretation, Statistical; D004574:Electromagnetic Fields; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods* / Q000706:statistics & numerical data; D007202:Indicators and Reagents; D009589:Nitrogen Oxides / Q000737:chemistry; D012815:Signal Processing, Computer-Assisted; D013113:Spin Labels
chemical_list: D003497:Cyclic N-Oxides; D007202:Indicators and Reagents; D009589:Nitrogen Oxides; D013113:Spin Labels; C001803:tempol