title: First-order multivariate calibration applied to the simultaneous fluorometric determination of the anticancer agents CPT-11 and SN-38 in serum and urine samples.
abstract: The first-order multivariate calibration method was used for the simultaneous determination of the camptothecin derivative, CPT-11 and its main metabolite, SN-38. The method is based on the fluorescence emission of these compounds in acidic media (acetate buffer, pH 4.75) and in the presence of ethanol (50%). The experimental calibration matrix was constructed with 12 samples using a central composite design. The cross-validation method was used for selecting the optimum number of factors. The results showed that simultaneous determination could be performed in the range 0.08 - 1.50 and 0.10 - 0.40 µg mL(-1) for CPT-11 and SN-38, respectively. The method was successfully applied to the simultaneous determination of both analytes in human urine and in serum samples previously extracted with chloroform.
authors: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain. iduran@unex.es
authors: Isabel Durán
authors: ID
authors: Martín-Merás
authors: María Isabel
authors: MI
authors: Rodríguez-Cáceres
authors: María del Carmen
authors: Mdel C
authors: Hurtado-Sánchez
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood / Q000737:chemistry / Q000652:urine; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000652:urine; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D000077146:Irinotecan; D015999:Multivariate Analysis; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D000077146:Irinotecan; D002166:Camptothecin