title: Trypanosoma cruzi DNA replication includes the sequential recruitment of pre-replication and replication machineries close to nuclear periphery.
abstract: In eukaryotes, many nuclear processes are spatially compartmentalized. Previously, we have shown that in Trypanosoma cruzi, an early-divergent eukaryote, DNA replication occurs at the nuclear periphery where chromosomes remain constrained during the S phase of the cell cycle. We followed Orc1/Cdc6, a pre-replication machinery component and the proliferating cell nuclear antigen (PCNA), a component of replication machinery, during the cell cycle of this protozoon. We found that, at the G(1) stage, TcOrc1/Cdc6 and TcPCNA are dispersed throughout the nuclear space. During the G(1)/S transition, TcOrc1/Cdc6 migrates to a region close to nuclear periphery. At the onset of S phase, TcPCNA is loaded onto the DNA and remains constrained close to nuclear periphery. Finally, in G(2), mitosis and cytokinesis, TcOrc1/Cdc6 and TcPCNA are dispersed throughout the nuclear space. Based on these findings, we propose that DNA replication in T. cruzi is accomplished by the organization of functional machineries in a spatial-temporal manner.
authors: Laboratório de Parasitologia, Instituto Butantan, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Simone Guedes
authors: SG
authors: Calderano
authors: Patrícia Diogo
authors: PD
authors: de Melo Godoy
authors: Maria Cristina M
authors: MC
authors: Motta
authors: Renato A
authors: RA
authors: Mortara
authors: Sergio
authors: S
authors: Schenkman
authors: M Carolina
authors: MC
authors: Elias
mesh_terms: D021581:Active Transport, Cell Nucleus; D018797:Cell Cycle Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D016054:DNA, Protozoan / Q000096:biosynthesis* / Q000378:metabolism; D016193:G1 Phase; D051738:Origin Recognition Complex / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism; D016196:S Phase; D014349:Trypanosoma cruzi / Q000166:cytology* / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D016054:DNA, Protozoan; D051738:Origin Recognition Complex; D018809:Proliferating Cell Nuclear Antigen; D015800:Protozoan Proteins