title: An ATP-binding cassette subfamily G full transporter is essential for the retention of leaf water in both wild barley and rice.
abstract: Land plants have developed a cuticle preventing uncontrolled water loss. Here we report that an ATP-binding cassette (ABC) subfamily G (ABCG) full transporter is required for leaf water conservation in both wild barley and rice. A spontaneous mutation, eibi1.b, in wild barley has a low capacity to retain leaf water, a phenotype associated with reduced cutin deposition and a thin cuticle. Map-based cloning revealed that Eibi1 encodes an HvABCG31 full transporter. The gene was highly expressed in the elongation zone of a growing leaf (the site of cutin synthesis), and its gene product also was localized in developing, but not in mature tissue. A de novo wild barley mutant named "eibi1.c," along with two transposon insertion lines of rice mutated in the ortholog of HvABCG31 also were unable to restrict water loss from detached leaves. HvABCG31 is hypothesized to function as a transporter involved in cutin formation. Homologs of HvABCG31 were found in green algae, moss, and lycopods, indicating that this full transporter is highly conserved in the evolution of land plants.
authors: Chinese Academy of Sciences, Lanzhou 730000, China. guoxiong@lzb.ac.cn
authors: Guoxiong
authors: G
authors: Chen
authors: Takao
authors: T
authors: Komatsuda
authors: Jian Feng
authors: JF
authors: Ma
authors: Christiane
authors: C
authors: Nawrath
authors: Mohammad
authors: M
authors: Pourkheirandish
authors: Akemi
authors: A
authors: Tagiri
authors: Yin-Gang
authors: YG
authors: Hu
authors: Mohammad
authors: M
authors: Sameri
authors: Xinrong
authors: X
authors: Li
authors: Xin
authors: X
authors: Zhao
authors: Yubing
authors: Y
authors: Liu
authors: Chao
authors: C
authors: Li
authors: Xiaoying
authors: X
authors: Ma
authors: Aidong
authors: A
authors: Wang
authors: Sudha
authors: S
authors: Nair
authors: Ning
authors: N
authors: Wang
authors: Akio
authors: A
authors: Miyao
authors: Shun
authors: S
authors: Sakuma
authors: Naoki
authors: N
authors: Yamaji
authors: Xiuting
authors: X
authors: Zheng
authors: Eviatar
authors: E
authors: Nevo
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D055864:Droughts; D019143:Evolution, Molecular; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics / Q000378:metabolism*; D008563:Membrane Lipids / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D008563:Membrane Lipids; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014867:Water; C000521:cutin