title: Functional analysis of the two Brassica AP3 genes involved in apetalous and stamen carpelloid phenotypes.
abstract: The Arabidopsis homeotic genes APETALA3 (AP3) and PISTILLATA (PI) are B genes which encode MADS-box transcription factors and specify petal and stamen identities. In the current study, the stamen carpelloid (SC) mutants, HGMS and AMS, of B. rapa and B. napus were investigated and two types of AP3 genes, B.AP3.a and B.AP3.b, were functional characterized. B.AP3.a and B.AP3.b share high similarity in amino acid sequences except for 8 residues difference located at the C-terminus. Loss of this 8 residues in B.AP3.b led to the change of PI-derived motifs. Meanwhile, B.AP3.a specified petal and stamen development, whereas B.AP3.b only specified stamen development. In B. rapa, the mutations of both genes generated the SC mutant HGMS. In B. napus that contained two B.AP3.a and two B.AP3.b, loss of the two B.AP3.a functions was the key reason for the apetalous mutation, however, the loss-of-function in all four AP3 was related to the SC mutant AMS. We inferred that the 8 residues or the PI-derived motif in AP3 gene probably relates to petal formation.
authors: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi, China.
authors: Yanfeng
authors: Y
authors: Zhang
authors: Xuefang
authors: X
authors: Wang
authors: Wenxue
authors: W
authors: Zhang
authors: Fei
authors: F
authors: Yu
authors: Jianhua
authors: J
authors: Tian
authors: Dianrong
authors: D
authors: Li
authors: Aiguang
authors: A
authors: Guo
mesh_terms: D001937:Brassica / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D035264:Flowers / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism
chemical_list: D010940:Plant Proteins