title: Stromal cell-derived factor-1 significantly induces proliferation, migration, and collagen type I expression in a human periodontal ligament stem cell subpopulation.
abstract: BACKGROUND
  The pivotal role of chemokine stromal cell-derived factor-1 (SDF-1) in bone marrow mesenchymal stem cells recruitment and tissue regeneration has already been reported. However, its roles in human periodontal ligament stem cells (PDLSCs) remain unknown. PDLSCs are regarded as candidates for periodontal tissue regeneration and are used in stem cell-based periodontal tissue engineering. The expression of chemokine receptors on PDLSCs and the migration of these cells induced by chemokines and their subsequent function in tissue repair may be a crucial procedure for periodontal tissue regeneration.
  
  
  METHODS
  PDL tissues were obtained from clinically healthy premolars extracted for orthodontic reasons and used to isolate single-cell colonies by the limited-dilution method. Immunocytochemical staining was used to detect the expression of the mesenchymal stem cell marker STRO-1. Differentiation potentials were assessed by alizarin-red staining and oil-red O staining. The expression of SDF-1 receptor CXCR4 was evaluated by real-time polymerase chain reaction (PCR) and immunocytochemical staining. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and bromodeoxyuridine incorporation assay were used to determine the viability and proliferation of the PDLSC subpopulation. Expression of collagen type I and alkaline phosphatase was detected by real-time PCR to determine the effect of SDF-1 on cells differentiation.
  
  
  RESULTS
  Twenty percent of PDL single-cell colonies expressed STRO-1 positively, and this specific subpopulation was positive for CXCR4 and formed minerals and lipid vacuoles after 4 weeks induction. SDF-1 significantly increased proliferation and stimulated the migration of this PDLSC subpopulation at concentrations between 100 and 400 ng/mL. CXCR4 neutralizing antibody could block cell proliferation and migration, suggesting that SDF-1 exerted its effects on cells through CXCR4. SDF-1 promoted collagen type I level significantly but had little effect on alkaline phosphatase level.
  
  
  CONCLUSIONS
  SDF-1 may have the potential of promoting periodontal tissue regeneration by the mechanism of guiding PDLSCs to destructive periodontal tissue, promoting their activation and proliferation and influencing the differentiation of these stem cells.
authors: Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China.
authors: Lingqian
authors: L
authors: Du
authors: Pishan
authors: P
authors: Yang
authors: Shaohua
authors: S
authors: Ge
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis / Q000187:drug effects*; D006801:Humans; D008055:Lipids / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology*; D019718:Receptors, CXCR4 / Q000032:analysis / Q000502:physiology; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D000954:Antigens, Surface; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D024042:Collagen Type I; D008055:Lipids; D019718:Receptors, CXCR4; C525591:STRO-1 antigen, human; D000469:Alkaline Phosphatase