title: Gene expression profiling in male genital lichen sclerosus.
abstract: Male genital lichen sclerosus (MGLSc) has a bimodal distribution in boys and men. It is associated with squamous cell carcinoma (SCC). The pathogenesis of MGLSc is unknown. HPV and autoimmune mechanisms have been mooted. Anti extracellular matrix protein (ECM)1 antibodies have been identified in women with GLSc. The gene expression pattern of LSc is unknown. Using DNA microarrays we studied differences in gene expression in healthy and diseased prepuces obtained at circumcision in adult males with MGLSc (n = 4), paediatric LSc (n = 2) and normal healthy paediatric foreskin (n = 4). In adult samples 51 genes with significantly increased expression and 87 genes with significantly reduced expression were identified; paediatric samples revealed 190 genes with significantly increased expression and 148 genes with significantly reduced expression. Concordance of expression profiles between adult and paediatric samples indicates the same disease process. Functional analysis revealed increased expression in the adult and child MGSLc samples in the immune response/cellular defence gene ontology (GO) category and reduced expression in other categories including genes related to squamous cancer. No specific HPV, autoimmune or squamous carcinogenesis-associated gene expression patterns were found. ECM1 and CABLES1 expression were significantly reduced in paediatric and adult samples respectively.
authors: Dermatology/Chelsea & Westminster Campus, Imperial College School of Medicine, London, UK.
authors: Emma
authors: E
authors: Edmonds
authors: Geraint
authors: G
authors: Barton
authors: Sandrine
authors: S
authors: Buisson
authors: Nick
authors: N
authors: Francis
authors: Frances
authors: F
authors: Gotch
authors: Laurence
authors: L
authors: Game
authors: Munther
authors: M
authors: Haddad
authors: Michael
authors: M
authors: Dinneen
authors: Chris
authors: C
authors: Bunker
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism; D052816:Foreskin / Q000378:metabolism* / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D018459:Lichen Sclerosus et Atrophicus / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011230:Precancerous Conditions / Q000235:genetics / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics / Q000378:metabolism
chemical_list: C486568:CABLES1 protein, human; D002352:Carrier Proteins; D016213:Cyclins; C490607:ECM1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins