title: Differential expression of muscarinic acetylcholine receptor subtypes in Jurkat cells and their signaling.
abstract: Muscarinic acetylcholine receptors expression and signaling in the human Jurkat T cell line were investigated. Semiquantitative real-time PCR and radioligand binding studies, using a wide set of antagonist compounds, showed the co-existence of M(3), M(4), and M(5) subtypes. Stimulation of these subpopulations caused a concentration and time- dependent activation of second messengers and ERK signaling pathways, with a major contribution of the M(3) subtype in a G(q/11)-mediated response. In addition, we found that T-cell stimulation leads to increased expression of M(3) and M(5) both at transcriptional and protein levels in a PLC/PKCθ dependent manner. Our data clarifies the functional role of AChR subtypes in Jurkat cells and pave the way to future studies on the potential cross-talk among these subpopulations and their regulation of T lymphocytes immune function.
authors: Centre for Molecular Biotechnology, Department of Chemical Engineering, Technical University of Catalonia, Barcelona, Spain. m.perez@aston.ac.uk
authors: Mileidys Perez
authors: MP
authors: Alea
authors: Dasiel O
authors: DO
authors: Borroto-Escuela
authors: Wilber
authors: W
authors: Romero-Fernandez
authors: Kjell
authors: K
authors: Fuxe
authors: Pere
authors: P
authors: Garriga
mesh_terms: D006801:Humans; D019169:Jurkat Cells; D018727:Muscarinic Antagonists / Q000494:pharmacology; D043643:Receptor, Muscarinic M1 / Q000096:biosynthesis / Q000235:genetics; D043585:Receptor, Muscarinic M2 / Q000096:biosynthesis / Q000235:genetics; D043587:Receptor, Muscarinic M3 / Q000096:biosynthesis / Q000235:genetics; D043588:Receptor, Muscarinic M4 / Q000096:biosynthesis / Q000235:genetics; D043642:Receptor, Muscarinic M5 / Q000096:biosynthesis / Q000235:genetics; D011976:Receptors, Muscarinic / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D018727:Muscarinic Antagonists; D043643:Receptor, Muscarinic M1; D043585:Receptor, Muscarinic M2; D043587:Receptor, Muscarinic M3; D043588:Receptor, Muscarinic M4; D043642:Receptor, Muscarinic M5; D011976:Receptors, Muscarinic