title: Coherent Raman scanning fiber endoscopy.
abstract: Coherent Raman scattering methods allow for label-free imaging of tissue with chemical contrast and high spatial and temporal resolution. However, their imaging depth in scattering tissue is limited to less than 1 mm, requiring the development of endoscopes to obtain images deep inside the body. Here, we describe a coherent Raman endoscope that provides stimulated Raman scattering images at seven frames per second using a miniaturized fiber scanner, a custom-designed objective lens, and an optimized scheme for collection of scattered light from the tissue. We characterize the system and demonstrate chemical selectivity in mouse tissue images.
authors: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
authors: Brian G
authors: BG
authors: Saar
authors: Richard S
authors: RS
authors: Johnston
authors: Christian W
authors: CW
authors: Freudiger
authors: X Sunney
authors: XS
authors: Xie
authors: Eric J
authors: EJ
authors: Seibel
mesh_terms: D000818:Animals; D004724:Endoscopy / Q000379:methods*; D051379:Mice; D012867:Skin; D013059:Spectrum Analysis, Raman / Q000379:methods*