title: Identification of C4 photosynthesis metabolism and regulatory-associated genes in Eleocharis vivipara by SSH.
abstract: This is the first effort to investigate the candidate genes involved in kranz developmental regulation and C(4) metabolic fluxes in Eleocharis vivipara, which is a leafless freshwater amphibious plant and possesses a distinct culms anatomy structure and photosynthetic pattern in contrasting environments. A terrestrial specific SSH library was constructed to investigate the genes involved in kranz anatomy developmental regulation and C(4) metabolic fluxes. A total of 73 ESTs and 56 unigenes in 384 clones were identified by array hybridization and sequencing. In total, 50 unigenes had homologous genes in the databases of rice and Arabidopsis. The real-time quantitative PCR results showed that most of the genes were accumulated in terrestrial culms and ABA-induced culms. The C(4) marker genes were stably accumulated during the culms development process in terrestrial culms. With respect to C(3) culms, C(4) photosynthesis metabolism consumed much more transporters and translocators related to ion metabolism, organic acids and carbohydrate metabolism, phosphate metabolism, amino acids metabolism, and lipids metabolism. Additionally, ten regulatory genes including five transcription factors, four receptor-like proteins, and one BURP protein were identified. These regulatory genes, which co-accumulated with the culms developmental stages, may play important roles in culms structure developmental regulation, bundle sheath chloroplast maturation, and environmental response. These results shed new light on the C(4) metabolic fluxes, environmental response, and anatomy structure developmental regulation in E. vivipara.
authors: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors: Taiyu
authors: T
authors: Chen
authors: Rongjian
authors: R
authors: Ye
authors: Xiaolei
authors: X
authors: Fan
authors: Xianghua
authors: X
authors: Li
authors: Yongjun
authors: Y
authors: Lin
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D005075:Biological Evolution; D002244:Carbon / Q000378:metabolism*; D004583:Electrons; D031256:Eleocharis / Q000033:anatomy & histology / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D008027:Light; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D009693:Nucleic Acid Hybridization; D010788:Photosynthesis / Q000235:genetics* / Q000528:radiation effects; D010937:Plant Growth Regulators / Q000378:metabolism; D018547:Plant Stems / Q000033:anatomy & histology / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects; D015854:Up-Regulation / Q000528:radiation effects; D014867:Water
chemical_list: D026901:Membrane Transport Proteins; D010937:Plant Growth Regulators; D018749:RNA, Plant; D014867:Water; D000040:Abscisic Acid; D002244:Carbon