title: Host-dependent suppression of RNA silencing mediated by the viral suppressor p19 in potato.
abstract: p19 protein encoded by tomato bushy stunt virus (TBSV) is known as a suppressor of RNA silencing via inhibition of small RNA-guided cleavage in plants. In this study, we generated TBSVp19-expressing patatin-RNAi transgenic potatoes to identify the inhibitory mechanisms of RNA silencing mediated by TBSVp19. In TBSVp19-expressing patatin-RNAi lines, reduction of patatin-derived siRNA accumulation and complementation of patatin transcripts were detected in comparison with the non-TBSVp19-expressing patatin-RNAi line, suggesting that TBSVp19 suppresses the siRNA-mediated silencing pathway. Interestingly, no apparent effect on the accumulation of miRNA168 and other miRNAs was detected in TBSVp19-expressing lines; previous studies reported that p19 induced the accumulation of both miRNA168 and its target Argonaute 1 (AGO1) mRNA, but suppressed AGO1 translation via up-regulation of miRNA168 in Arabidopsis. In addition, the expression of Argonaute 1 (AGO1-1 and AGO1-2) and Dicer-like 1 (DCL1) was not significantly altered in p19-expressing lines. Interestingly, no translational inhibition of AGO1 mediated by p19 was detected. These results suggest that p19 suppresses siRNA-mediated silencing in potato, but may not affect miRNA-mediated silencing, possibly due to the host-dependent manner of p19 activity.
authors: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, Korea.
authors: Joon-Woo
authors: JW
authors: Ahn
authors: Jong Suk
authors: JS
authors: Lee
authors: Seyed Javad
authors: SJ
authors: Davarpanah
authors: Jae-Heung
authors: JH
authors: Jeon
authors: Youn-Il
authors: YI
authors: Park
authors: Jang Ryol
authors: JR
authors: Liu
authors: Won Joong
authors: WJ
authors: Jeong
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D015152:Blotting, Northern; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism; D017864:Tombusvirus / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; C102165:DCL protein, Lycopersicon esculentum; D035683:MicroRNAs; C493885:P19 protein, tomato bushy stunt virus; D010940:Plant Proteins; D034741:RNA, Small Interfering; D014764:Viral Proteins; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases