title: Biosynthesis and secretion of mouse cysteine-rich with EGF-like domains 2.
abstract: In this study, we found that Cysteine-rich with EGF-like domains 2 (CRELD2), a novel endoplasmic reticulum stress-inducible protein, is not only localized in the ER-Golgi apparatus but also spontaneously secreted. Deletion of four C-terminal amino acids from mouse CRELD2 or addition of tag-peptides to its C-terminus dramatically enhanced CRELD2 secretion. Intra- and extra-cellular CRELD2 is differentially glycosylated and its spontaneous secretion was significantly prevented by overexpression of a dominant negative mutant Sar1 and treatment with brefeldin A. Overexpression of wild-type GRP78 remarkably enhanced the secretion of wild-type but not mutant CRELD2. Our results demonstrate both that CRELD2 is a novel secretory glycoprotein regulated by Sar1 and GRP78 and that the C-terminal of CRELD2 plays a crucial role in its secretion.
authors: Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu, Japan. oohashi@gifu-u.ac.jp
authors: Kentaro
authors: K
authors: Oh-hashi
authors: Ryosuke
authors: R
authors: Kunieda
authors: Yoko
authors: Y
authors: Hirata
authors: Kazutoshi
authors: K
authors: Kiuchi
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000378:metabolism; D006023:Glycoproteins; D006031:Glycosylation; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics / Q000502:physiology
chemical_list: C561361:CRELD2 protein, mouse; D015815:Cell Adhesion Molecules; D000091342:Endoplasmic Reticulum Chaperone BiP; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D020559:Monomeric GTP-Binding Proteins