title: Functionalized silica-based nanoparticles for photodynamic therapy.
abstract: OBJECTIVE
  The strategy developed aims to favor the vascular effect of photodynamic therapy by targeting tumor-associated vascularization using peptide-functionalized nanoparticles. We previously described the conjugation of a photosensitizer to a peptide targeting neuropilin-1 overexpressed in tumor angiogenic vessels.
  
  
  METHODS
  In this study, we have designed and photophysically characterized a multifunctional nanoparticle consisting of a surface-localized tumor vasculature targeting peptides and encapsulated photodynamic therapy and imaging agents.
  
  
  CONCLUSIONS
  The elaboration of these multifunctional silica-based nanoparticles is reported. Nanoparticles functionalized with approximately 4.2 peptides bound to recombinant neuropilin-1 protein. Nanoparticles conferred photosensitivity to cells overexpressing neuropilin-1, providing evidence that the chlorin grafted within the nanoparticle matrix can be photoactivated to yield photocytotoxic effects in vitro.
authors: Laboratoire Réactions et Génie des Procédés (LRGP), Nancy-University, CNRS, Nancy, France.
authors: Pierre
authors: P
authors: Couleaud
authors: Denise
authors: D
authors: Bechet
authors: Régis
authors: R
authors: Vanderesse
authors: Muriel
authors: M
authors: Barberi-Heyob
authors: Anne-Charlotte
authors: AC
authors: Faure
authors: Stéphane
authors: S
authors: Roux
authors: Olivier
authors: O
authors: Tillement
authors: Sabine
authors: S
authors: Porhel
authors: François
authors: F
authors: Guillemin
authors: Céline
authors: C
authors: Frochot
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D015394:Molecular Structure; D053758:Nanoparticles / Q000737:chemistry*; D039942:Neuropilin-1 / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D010455:Peptides; D017319:Photosensitizing Agents; D011994:Recombinant Proteins; D039942:Neuropilin-1; D012822:Silicon Dioxide