title: Case report: detection of rotavirus RNA in the cerebrospinal fluid of a child with rotavirus gastroenteritis and meningism.
abstract: Although case reports have described detection of rotavirus (RV) in extraintestinal sites such as the liver, kidney, and central nervous system (CNS) of children with RV gastroenteritis, CNS localization in RV infection seems to be rare. RT-PCR and nucleotide sequencing detected a G1P[8] strain in the stool and cerebrospinal fluid (CSF) samples of a patient with concurrent RV-associated enteritis and CNS signs. Upon sequence analysis, the viruses detected in the CSF was identical to the virus detected in the stools. In the VP7- and VP4-based phylogenetic dendograms the strain clustered within the G1-Ic sub-lineage and the P[8]-III lineage. This study supports the hypothesis that RV infection was able to spread from the intestinal tract to the CNS, and likely played a role in the onset of neurological disease.
authors: Section of Microbiology, Department of Pathology and Laboratory Medicine, School of Medicine, University of Parma, Parma, Italy. mariacristina.medici@unipr.it
authors: Maria Cristina
authors: MC
authors: Medici
authors: Laura Anna
authors: LA
authors: Abelli
authors: Paola
authors: P
authors: Guerra
authors: Icilio
authors: I
authors: Dodi
authors: Giuseppe
authors: G
authors: Dettori
authors: Carlo
authors: C
authors: Chezzi
mesh_terms: D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D002555:Cerebrospinal Fluid / Q000821:virology*; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000134:cerebrospinal fluid / Q000821:virology*; D006801:Humans; D008580:Meningism / Q000134:cerebrospinal fluid / Q000821:virology*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000134:cerebrospinal fluid*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000821:virology*; D017423:Sequence Analysis, RNA
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus