title: Calcineurin colocalizes with P-bodies and stress granules during thermal stress in Cryptococcus neoformans.
abstract: Calcineurin is a calcium-calmodulin-activated serine/threonine-specific phosphatase that operates during cellular responses to stress and plays a prominent role in transcriptional control, whereas regulatory events beyond transcription are less well characterized. This study reveals a novel transcription-independent role of calcineurin during the temperature stress response in the human fungal pathogen Cryptococcus neoformans. The diffusely cytoplasmic calcineurin catalytic subunit Cna1 relocates to endoplasmic reticulum (ER)-associated puncta and the mother-bud neck when cells are subjected to 37°C. More than 50% of Cna1 puncta contain the P-body constituent decapping enzyme Dcp1 and the stress granule constituent poly(A)-binding protein Pub1. These results support a model in which calcineurin orchestrates thermal stress responses by associating with sites of mRNA processing.
authors: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
authors: Lukasz
authors: L
authors: Kozubowski
authors: Eanas F
authors: EF
authors: Aboobakar
authors: Maria E
authors: ME
authors: Cardenas
authors: Joseph
authors: J
authors: Heitman
mesh_terms: D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D003455:Cryptococcus neoformans / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006358:Hot Temperature; D039102:Poly(A)-Binding Proteins / Q000378:metabolism; D013312:Stress, Physiological; D014158:Transcription, Genetic
chemical_list: D039102:Poly(A)-Binding Proteins; D019703:Calcineurin; D010450:Endopeptidases; C020071:dipeptidyl carboxypeptidase