title: Antitumor effect of aspirin in glioblastoma cells by modulation of β-catenin/T-cell factor-mediated transcriptional activity.
abstract: OBJECTIVE
  The goal in this study was to investigate the antitumor effect of aspirin in glioblastoma cells and the molecular mechanism involved in its antineoplastic activities.
  
  
  METHODS
  The authors used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, flow cytometry, the annexin V method, and Transwell cell invasion test to detect the proliferation and invasive activity of U87 and A172 glioma cells before and after being treated with aspirin. To determine the effects of aspirin on β-catenin/T-cell factor (TCF) transcription activity, reporter constructs containing 3 repeats of the wild-type (TOPflash) or mutant (FOPflash) TCF-binding sites were used. Reverse transcriptase polymerase chain reaction and Western blot analyses were used to detect the expression of multiple β-catenin/TCF target genes following aspirin treatment.
  
  
  RESULTS
  The transcriptional activity of the β-catenin/TCF complex was strongly inhibited by aspirin. Increasing the concentration of aspirin resulted in decreased expression of c-myc, cyclin D1, and fra-1 mRNA and protein in U87 and A172 cells in a dose-dependent manner. Aspirin inhibited glioma cell proliferation and invasive ability, and induced apoptotic cell death.
  
  
  CONCLUSIONS
  The results suggest that aspirin is a potent antitumor agent, and that it exerts its antineoplastic action by inhibition of the β-catenin/TCF signaling pathway in glioma cells.
authors: Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Taijin, People’s Republic of China.
authors: Fengming
authors: F
authors: Lan
authors: Xiao
authors: X
authors: Yue
authors: Lei
authors: L
authors: Han
authors: Xubo
authors: X
authors: Yuan
authors: Zhendong
authors: Z
authors: Shi
authors: Kai
authors: K
authors: Huang
authors: Yang
authors: Y
authors: Yang
authors: Jian
authors: J
authors: Zou
authors: Junxia
authors: J
authors: Zhang
authors: Tao
authors: T
authors: Jiang
authors: Peiyu
authors: P
authors: Pu
authors: Chunsheng
authors: C
authors: Kang
mesh_terms: D001241:Aspirin / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D051820:TCF Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: D016861:Cyclooxygenase Inhibitors; D051820:TCF Transcription Factors; D051176:beta Catenin; D001241:Aspirin