title: Interaction between myosin and a trace amount of caldesmon.
abstract: Caldesmon (CaD) is known as an actin binding protein. In this study, we proposed that a trace amount of caldesmon (TACD) could highly, efficiently, interact with myosin by producing a 'domino-like cascade' and characterized that TACD (lowest caldesmon/myosin molar ratio: 1/10,000) significantly increased precipitations and intrinsic tryptophan fluorescence intensity of myosin in both phosphorylated and unphosphorylated states compared to the base controls (P < 0.01). Actin-blocked TACD-myosin interaction, suggesting that it functioned as a negative regulator. Since CaD is not an enzyme, the in vivo significance of the highly efficient TACD-myosin interaction needs further investigation.
authors: Department of Pharmacology, Dalian Medical University, Dalian, China 116044.
authors: Ying
authors: Y
authors: Zhang
authors: Ze-Yao
authors: ZY
authors: Tang
authors: Kazuhiro
authors: K
authors: Kohama
authors: Yuan
authors: Y
authors: Lin
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D002645:Chickens; D005895:Gizzard, Avian / Q000737:chemistry; D059165:Hormesis; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D014335:Tropomyosin / Q000737:chemistry / Q000378:metabolism
chemical_list: D000199:Actins; D002148:Calmodulin-Binding Proteins; D014335:Tropomyosin; D009219:Myosin-Light-Chain Kinase; D009218:Myosins