title: Novel Sample Preparation for Mass Spectral Analysis of Complex Biological Samples.
abstract: The ability to combine a selective capture strategy with on chip MALDI-TOF analysis allows for rapid, sensitive analysis of a variety of different analytes. In this overview a series of applications of capture enhanced laser desorption ionization time of flight (CELDI-TOF) mass spectrometry are described. The key feature of the assay is an off-chip capture step that utilizes high affinity bacterial binding proteins to capture a selected ligand. This allows large volumes of sample to be used and provides for a concentration step prior to transfer to a gold chip for traditional mass spectral analysis. The approach can also be adapted to utilize specific antibody as the basis of the capture step. The direct and indirect CELDI-TOF assays are rapid, reproducible and can be a valuable proteomic tool for analysis of low abundance molecules present in complex mixtures like blood plasma.
authors: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27707.
authors: Eric A
authors: EA
authors: Porsch
authors: Cecelia A
authors: CA
authors: Shertz
authors: Michael D
authors: MD
authors: Boyle