title: Novel polymorphic microsatellite loci for the Korean Black Scraper (Thamnaconus modestus), and their application to the genetic characterization of wild and farmed populations.
abstract: In this study, we developed 20 polymorphic microsatellite markers for the Korean black scraper, Thamnaconus modestus (Günther, 1877), Monacanthidae, and used them to compare allelic variation between wild and hatchery populations in Korea. All loci were readily amplified and demonstrated allelic variability, with the number of alleles ranging from 5-35 in the wild population and 5-22 in the farmed population. The average observed and expected heterozygosities were estimated, respectively, as 0.74 and 0.80 in the hatchery samples and 0.78 and 0.81 in the wild ones. These results indicate lower genetic variability in the hatchery population than in the wild population and minor, but significant, genetic differentiation between the two populations (F(ST) = 0.005, P < 0.01). Additionally, cross-amplification was tested in another monacanthid species, Stephanolepis cirrhifer; many loci were found that yielded useful information. The high degree of polymorphism exhibited by the 20 microsatellites will be useful in future aquaculture and population genetic studies for developing conservation and management plans.
authors: New Strategy Research Center, National Fisheries Research and Development Institute, Busan 619-705, Korea; E-Mails: bilee@nfrdi.go.kr (B.I.L.); yckim@nfrdi.go.kr (Y.C.K.).
authors: Hye Suck
authors: HS
authors: An
authors: Eun Mi
authors: EM
authors: Kim
authors: Jang Wook
authors: JW
authors: Lee
authors: Chun Mae
authors: CM
authors: Dong
authors: Bai Ik
authors: BI
authors: Lee
authors: Yi Cheong
authors: YC
authors: Kim
mesh_terms: D000483:Alleles; D000818:Animals; D017756:Aquaculture; D005787:Gene Frequency; D015723:Gene Library; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D056910:Republic of Korea; D023721:Tetraodontiformes / Q000235:genetics*