title: D-Ser-containing humanin shows promotion of fibril formation.
abstract: Humanin (HN), a peptide of 24 amino acid residues, suppresses the neuronal cell death that is induced by the gene products of Alzheimer's disease. HN contains two Ser residues at positions 7 and 14. Because the proportion of D-Ser isomerized from L-Ser in proteins appears to increase as cellular organs age, we explored the structural effects of the isomerization of each Ser residue in HN. By using a thioflavin-T assay to detect fibril formation, we found that an HN derivative that contained two isomerized D-Ser residues had a greater tendency to form fibrils than did wild-type HN or HNs containing single D-Ser residues. A previous report showed that HN containing two D-Ser residues exerts neuroprotective activity. Our data, therefore, suggest that the fibril formation by HN that contains two D-Ser residues may promote HN neuroprotective activity.
authors: Department of Physical Chemistry, Research Institute of Pharmaceutical Sciences, Musashino University, 1-1-20 Shinmachi, Nishitokyo, Tokyo, 202-8585, Japan.
authors: Kanehiro
authors: K
authors: Hayashi
authors: Jumpei
authors: J
authors: Sasabe
authors: Tomohiro
authors: T
authors: Chiba
authors: Sadakazu
authors: S
authors: Aiso
authors: Naoko
authors: N
authors: Utsunomiya-Tate
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000595:Amino Acid Sequence; D052160:Benzothiazoles; D002942:Circular Dichroism; D003224:Congo Red; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D018696:Neuroprotective Agents / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012996:Solutions; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D012996:Solutions; D013844:Thiazoles; C427211:humanin; C009462:thioflavin T; D003224:Congo Red