title: Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes.
abstract: A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.
authors: Department of Animal Pathology, Faculty of Veterinary Medicine, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy.
authors: Sara
authors: S
authors: Lomonaco
authors: Stephen J
authors: SJ
authors: Knabel
authors: Alessandra
authors: A
authors: Dalmasso
authors: Tiziana
authors: T
authors: Civera
authors: Maria Teresa
authors: MT
authors: Bottero
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D057166:High-Throughput Screening Assays; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008088:Listeriosis / Q000175:diagnosis* / Q000382:microbiology*; D058889:Molecular Typing / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors