title: Induction of Epstein-Barr virus (EBV) lytic cycle in vitro causes lipid peroxidation, protein oxidation and DNA damage in lymphoblastoid B cell lines.
abstract: BACKGROUND
  We investigated the oxidative modifications of lipids, proteins and DNA, potential molecular targets of oxidative stress, in two lymphoblastoid cell lines: B95-8 and Raji, after EBV lytic cycle induction. Conjugated dienes level was measured as biomarker of lipid peroxidation. Malondialdehyde adduct and protein carbonyl levels, as well as protein thiol levels were measured as biomarkers of protein oxidation. DNA fragmentation was evaluated as biomarker of DNA oxidation.
  
  
  RESULTS
  After 48 h (peak of lytic cycle), a significant increase in conjugated dienes level was observed in B95-8 and Raji cell lines (p = 0.0001 and p = 0.019 respectively). Malondialdehyde adduct, protein carbonyl levels were increased in B95-8 and Raji cell lines after EBV lytic cycle induction as compared to controls (MDA-adduct: p = 0.008 and p = 0.006 respectively; Carbonyl: p = 0.003 and p = 0.0039 respectively). Proteins thiol levels were decreased by induction in B95-8 and Raji cell lines (p = 0.046; p = 0.002 respectively). DNA fragmentation was also detected in B95-8 and Raji cell lines after EBV lytic cycle induction as compared to controls.
  
  
  CONCLUSIONS
  The results of this study demonstrate the presence of increased combined oxidative modifications in lipids, proteins in B95-8 and Raji cells lines after EBV lytic cycle induction. These results suggest that lipid peroxidation, protein oxidation and DNA fragmentation are generally induced during EBV lytic cycle induction and probably contribute to the cytopathic effect of EBV.
authors: Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, Tunisia. bochra.gargouri@yahoo.fr.
authors: Bochra
authors: B
authors: Gargouri
authors: Rihab
authors: R
authors: Nasr
authors: Malek
authors: M
authors: Mseddi
authors: Riadh
authors: R
authors: Benmansour
authors: Saloua
authors: S
authors: Lassoued
mesh_terms: D000818:Animals; D002144:Callithrix; D002460:Cell Line; D004249:DNA Damage; D020031:Epstein-Barr Virus Infections / Q000503:physiopathology*; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D054448:Precursor Cells, B-Lymphoid / Q000821:virology*; D050050:Protein Carbonylation; D057074:Virus Release
chemical_list: D008315:Malondialdehyde