title: Therapeutic effect of antisecretory factor-rich egg yolk on the late phases of 2,4,6-trinitrobenzenesulphonic acid colitis in mice.
abstract: Antisecretory factor (AF) is expressed in all tissues of mammals, inhibits intestinal hypersecretion and has anti-inflammatory properties as well. Endogenous AF synthesis may be stimulated by feeding hydrothermally processed cereals. Alternatively, freeze-dried egg yolk can be used as a source of exogenous AF. Several reports have suggested that AF from freeze-dried egg yolk may be useful in inflammatory bowel disease. We assessed the effect of freeze-dried, AF-rich egg yolk intake on 2,4,6-trinitrobenzenesulphonic acid (TNBS) colitis. Balb/c mice were randomised to receive (1) AF in sterile drinking-water (4 g/l, n 38) and (2) sterile drinking-water alone (vehicle, n 38) from TNBS or saline administration onwards. Different subsets of mice were killed at weeks 1-3 after TNBS or saline administration. Macroscopic and microscopic damage was assessed in colonic specimens. Eicosanoid and cytokine production was evaluated in supernatants of 24 h-incubated colonic explants. Myeloperoxidase activity was measured in frozen colonic samples, while apoptosis was assessed in paraffined samples by the in situ oligoligation method. AF-treated mice showed a milder colonic damage compared with the vehicle group, which became statistically significant at week 3. This was accompanied by decreased IL-2, IL-1 and leukotriene B4 production at weeks 2 and 3, as well as increased interferon-γ at week 1, in AF-treated mice compared with vehicle-treated mice. AF-treated mice had significantly increased counts of apoptotic cells in the lamina propria at weeks 1 and 2 post-TNBS. In conclusion, the administration of AF-rich egg yolk has a therapeutic effect in the late phases of TNBS colitis in Balb/c mice.
authors: Gastroenterology and Nutrition Research Unit, Germans Trias i Pujol Research Institute in Health Sciences, Carretera del Canyet s/n, 08916 Badalona, Catalonia, Spain. jmanye.igtp.germanstrias@gencat.cat
authors: Josep
authors: J
authors: Mañé
authors: Violeta
authors: V
authors: Lorén
authors: Elisabet
authors: E
authors: Pedrosa
authors: Isabel
authors: I
authors: Ojanguren
authors: Eugeni
authors: E
authors: Domènech
authors: Miquel A
authors: MA
authors: Gassull
authors: Eduard
authors: E
authors: Cabré
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced*; D004530:Egg Yolk / Q000737:chemistry*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009479:Neuropeptides / Q000032:analysis / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*
chemical_list: D009479:Neuropeptides; C049628:antisecretory factor; D014302:Trinitrobenzenesulfonic Acid