title: Molecular cloning and polymorphism analysis of the prion protein gene in Tan sheep of Ningxia, China.
abstract: The resistance or susceptibility of sheep to scrapie is associated with polymorphisms of the prion protein gene (PRNP), particularly, single nucleotide polymorphisms (SNPs) in amino acid positions 136, 154 and 171. The prion protein (PrP) gene sequence and the deduced amino acid alignment of prion protein in Tan sheep, a local Chinese sheep breed traditionally raised in Ningxia, northwestern China, were determined and variability of the PrP amino acids sequence was analyzed in this study. The PrP nucleic acids and amino acids sequences of 112 Tan sheep were highly homogenous, although polymorphism of the PrP gene was detected at several sites, particularly codons 106, 154, and 171. The analysis of both sequences revealed that the most predominant allele at codons 136, 154 and 171 in Tan sheep was ARQ, which was known to be associated with high susceptibility to scrapie in sheep. The result suggests that Tan sheep is potentially susceptible to scrapie. Our findings provide valuable information for future breeding projects to scrapie resistance in Tan sheep.
authors: State Key Laboratories for Agrobiotechnology, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors: Lihua
authors: L
authors: Xu
authors: Zhuming
authors: Z
authors: Zhang
authors: Xiangmei
authors: X
authors: Zhou
authors: Xiaomin
authors: X
authors: Yin
authors: Lifeng
authors: L
authors: Yang
authors: Deming
authors: D
authors: Zhao
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001947:Breeding; D002681:China; D003062:Codon; D004198:Disease Susceptibility / Q000662:veterinary*; D020022:Genetic Predisposition to Disease; D008969:Molecular Sequence Data; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D012608:Scrapie / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D034561:Sheep, Domestic / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon; D011328:Prions