title: Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed.
abstract: One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.
authors: Key Laboratory of Chemical Safety and Health, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, People's Republic of China.
authors: P J
authors: PJ
authors: Luo
authors: W X
authors: WX
authors: Jiang
authors: X
authors: X
authors: Chen
authors: J Z
authors: JZ
authors: Shen
authors: Y N
authors: YN
authors: Wu
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D000900:Anti-Bacterial Agents; C035534:florfenicol; D013839:Thiamphenicol