title: Stephania delavayi Diels. inhibits breast carcinoma proliferation through the p38 MAPK/NF-κB/COX-2 pathway.
abstract: The nuclear factor κB (NF-κB)/inhibitor of κ kinase-β (IKKβ) signaling pathway is important in tumor promotion and progression. MDA-MB-231 human breast carcinoma cells express COX-2 and show a constitutive phosphorylation of NF-κB. Many non-specific inhibitors of IKKβ and NF-κB are used to inhibit tumor promotion and progression. The Stephania delavayi Diels. (S. delavayi Diels.) extract has been reported to safely activate B cell immunity and there is evidence suggesting that it may be a promising new anticancer therapeutic agent. S. delavayi Diels. extract suppressed proliferation of the breast cancer cell lines MDA-MB-231 and MCF-7 by inducing cell death. To aid in the development of the S. delavayi Diels. extract as a therapeutic agent, its mechanisms of action were investigated, in particular its effects on p38 MAPK, NF-κB and COX-2, which play important roles in inflammation and cancer. S. delavayi Diels. stimulated p38 MAPK phosphorylation but reduced NF-κB phosphorylation and COX-2 expression in a dose- and time-dependent manner. Thus, S. delavayi Diels., which appears to act primarily through p38 MAPK/NF-κB/COX-2 signaling in breast carcinomas, may be a potent anticancer agent with target specificity and low toxicity.
authors: Toxicology Center, Korea Institute of Toxicology, Yuseong, Daejeon 305-343, Republic of Korea.
authors: Dae-Hun
authors: DH
authors: Park
authors: Hong
authors: H
authors: De Xu
authors: Jaegal
authors: J
authors: Shim
authors: Yong-Chun
authors: YC
authors: Li
authors: Jong-Hwa
authors: JH
authors: Lee
authors: Soon-Chang
authors: SC
authors: Cho
authors: Sang-Seop
authors: SS
authors: Han
authors: Yun Lyul
authors: YL
authors: Lee
authors: Min-Jae
authors: MJ
authors: Lee
authors: Sung-Won
authors: SW
authors: Kwon
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000378:metabolism*; D005260:Female; D029624:Ferns / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D051546:Cyclooxygenase 2; D048051:p38 Mitogen-Activated Protein Kinases