title: Iron accumulation and DNA damage in a pig model of intracerebral hemorrhage.
abstract: Cerebral iron overload causes brain injury after intracerebral hemorrhage (ICH) in rats and pigs. The current study examined whether an iron chelator, deferoxamine, can reduce ICH-induced DNA damage in pigs. Pigs received an injection of autologous blood into the right frontal lobe. Deferoxamine (50 mg/kg, i.m.) or vehicle was given 2 h after ICH and then every 12 h up to 7 days. Animals were killed at day 3 or day 7 after ICH to examine iron accumulation and DNA damage. We found that ICH resulted in the development of a reddish perihematomal zone, with iron accumulation and DNA damage within that zone. Deferoxamine treatment reduced the perihematomal reddish zone, and the number of Perls' (p<0.01) and TUNEL (p<0.01) positive cells. In conclusion, iron accumulates in the perihematomal zone and causes DNA damage. Systemic deferoxamine treatment reduces ICH-induced iron overload and DNA damage in pigs.
authors: Department of Neurosurgery, University of Michigan, Ann Arbor, MI 48109-2200, USA.
authors: Yuxiang
authors: Y
authors: Gu
authors: Ya
authors: Y
authors: Hua
authors: Yangdong
authors: Y
authors: He
authors: Lin
authors: L
authors: Wang
authors: Hua
authors: H
authors: Hu
authors: Richard F
authors: RF
authors: Keep
authors: Guohua
authors: G
authors: Xi
mesh_terms: D000818:Animals; D002543:Cerebral Hemorrhage / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology*; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology*; D003676:Deferoxamine / Q000627:therapeutic use; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000187:drug effects / Q000473:pathology; D020287:In Situ Nick-End Labeling / Q000379:methods; D007501:Iron / Q000378:metabolism*; D008297:Male; D017262:Siderophores / Q000627:therapeutic use; D013552:Swine; D013997:Time Factors
chemical_list: D017262:Siderophores; D007501:Iron; D003676:Deferoxamine