title: Solid lipid nanoparticles loaded with anti-microRNA oligonucleotides (AMOs) for suppression of microRNA-21 functions in human lung cancer cells.
abstract: OBJECTIVE
  Literature has highlighted the practical use of solid lipid nanoparticles (SLNs) in research, but few reports have combined SLNs with miRNA-based therapy. We aimed to prepare SLNs to load anti-miRNA oligonucleotide (AMO) for miRNA-based therapy in vitro.
  
  
  METHODS
  SLNs were employed to encapsulate AMO by a solvent diffusion method, and then the properties of AMO-CLOSs (cationic lipid binded oligonucleotide (AMO)-loaded SLNs) were characterized. We studied cellular uptake and activation properties of AMO-CLOSs in A549 cells, including antisense efficiency, cell migration and invasion.
  
  
  RESULTS
  AMO-CLOSs were 187 nm in size and 46.6 mV in zeta potential with an approximately toroid morphology in the TEM image. AMO-CLOSs uptake by A549 cells was increased significantly higher and more effective than free AMO. Further results demonstrated that AMO-CLOSs showed high antisense efficiency of microRNA-21 and subsequently decreased the proliferation, migration and invasion of tumor cells.
  
  
  CONCLUSIONS
  These findings suggest that AMO-CLOSs represent a potential new approach for carrying anti-miRNA inhibitors for cancer therapy.
authors: Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors: San-Jun
authors: SJ
authors: Shi
authors: Zhi-Rong
authors: ZR
authors: Zhong
authors: Jie
authors: J
authors: Liu
authors: Zhi-Rong
authors: ZR
authors: Zhang
authors: Xun
authors: X
authors: Sun
authors: Tao
authors: T
authors: Gong
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000077192:Adenocarcinoma of Lung; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008175:Lung Neoplasms / Q000188:drug therapy*; D035683:MicroRNAs / Q000037:antagonists & inhibitors*; D053758:Nanoparticles / Q000737:chemistry; D009841:Oligonucleotides / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D008055:Lipids; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides