title: Chiral discrimination of drugs by DNA tetranucleotides under electrospray ionisation conditions.
abstract: The DNA tetranucleotides, extended versions of GCA at the 3'-end or 5'-end, were used as chiral selectors for the chiral discrimination of atenolol, DOPA, tamsulosin, valacyclovir and zolmitriptan. Chiral discrimination was achieved by investigating the collision-induced dissociation spectra of the [X+Y-2H](2-) ion generated by electrospraying a solution mixture of tetranucleotide (X) and R- or S-analyte drug (Y). The relative abundances of the precursor ion and the product ion, resulting from the loss of drug, were considered for measuring the degree of chiral discrimination. Among all the tetranucleotides studied, AGCA showed the highest chiral discrimination. The present study emphasised the position of an adenine base in the tetranucleotide in chiral discrimination. The suitability of the method for the measurement of optical purity was also demonstrated in the case of zolmitriptan.
authors: National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad-500 607, India.
authors: Tallagadda
authors: T
authors: Sivaleela
authors: Voleti
authors: V
authors: Nagaveni
authors: Sripadi
authors: S
authors: Prabhakar
authors: Mariappanadar
authors: M
authors: Vairamani
mesh_terms: D004247:DNA / Q000737:chemistry*; D016014:Linear Models; D009841:Oligonucleotides / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D009841:Oligonucleotides; D004364:Pharmaceutical Preparations; D004247:DNA