title: Automatic sample preparation of sulfonamide antibiotic residues in chicken breast muscle by using dynamic microwave-assisted extraction coupled with solid-phase extraction.
abstract: In the work, a rapid, simple and high-throughput sample preparation method was developed for the determination of sulfonamide (SA) antibiotic residues in chicken breast muscle. The extraction and clean-up were online combined and up to 20 samples can be treated simultaneously in 6 min. The SAs were first extracted with acetonitrile under the action of microwave energy, and then the extract was directly introduced into the SPE column for on-line clean-up and concentration. Subsequently, the SAs eluted from the SPE column were determined by liquid chromatography-tandem mass spectrometry. The precisions of extraction results of 20 samples were in the range of 4.9-7.4%. The limits of detection and quantification obtained were in the range of 2.4-3.6 ng/g and 8.6-11.3 ng/g for SAs, respectively. The recoveries of SAs obtained by analyzing chicken muscles at three fortified levels (10, 50 and 500 ng/g) were in the range of 82.6-93.2%. The results of the validation process prove that the proposed method is suitable for treating numbers of complex samples simultaneously in a short time.
authors: College of Chemistry, Jilin University, Changchun, PR China.
authors: Hui
authors: H
authors: Wang
authors: Yang
authors: Y
authors: Xu
authors: Weitao
authors: W
authors: Song
authors: Qi
authors: Q
authors: Zhao
authors: Xiaopan
authors: X
authors: Zhang
authors: Qinglei
authors: Q
authors: Zeng
authors: Haiyan
authors: H
authors: Chen
authors: Lan
authors: L
authors: Ding
authors: Nanqi
authors: N
authors: Ren
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D008872:Microwaves; D018482:Muscle, Skeletal / Q000737:chemistry*; D052616:Solid Phase Extraction; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides