title: Erythrocytic enzymes and antioxidant status in people with type 2 diabetes: beneficial effect of Syzygium cumini leaf extract in vitro.
abstract: The aim of the present study was to investigate the effects of Syzygium cumini leaf extract (ASc), on Adenosine deaminase (ADA) and Acetylcholinesterase (AChE) activities, and also on oxidative stress parameters in erythrocytes hemolysates (RBCs) and erythrocytes membranes (ghosts) from type 2 diabetics patients (Type 2 DM) under in vitro conditions. Non protein thiol groups (NP-SH), AChE, Catalase (CAT) and Superoxide Dismutase (SOD) activities were measure in RBCs. Further, ADA activity, Thiobarbituric Acid-Reactive Substances (TBARS) levels and protein thiol groups (P-SH) were estimated in ghosts. Also, P-SH and Vitamin C (VIT C) were measure in plasma sample. The results demonstrated that ADA and AChE activities, besides TBARS levels were higher in erythrocytes of Type 2 DM, while SOD activity and NP-SH levels were decreased when compared to control group. ASc, in vitro, reduced ADA and AChE activities and some parameters of oxidative stress. Furthermore, we observed correlations between VIT C and P-SH levels, ADA activity and P-SH levels, as well as NP-SH and TBARS levels in diabetics. The results suggest that ASc in vitro is able to promote the reduction of inflammation and oxidative stress parameters, and act against biochemical changes occurring in Diabetes mellitus (DM).
authors: Postgraduate Program in Pharmaceutical Sciences, Health Science Centre, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors: Karine Santos
authors: KS
authors: De Bona
authors: Luziane Potrich
authors: LP
authors: Bellé
authors: Paula Eliete Rodrigues
authors: PE
authors: Bittencourt
authors: Gabriela
authors: G
authors: Bonfanti
authors: Lariane Oliveira
authors: LO
authors: Cargnelluti
authors: Victor Camera
authors: VC
authors: Pimentel
authors: Amanda Roggia
authors: AR
authors: Ruviaro
authors: Maria Rosa Chitolina
authors: MR
authors: Schetinger
authors: Tatiana
authors: T
authors: Emanuelli
authors: Maria Beatriz
authors: MB
authors: Moretto
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D000110:Acetylcholinesterase; D000243:Adenosine Deaminase