title: [The hyposensitization of oval cell to antiproliferative effect of transforming growth factor β1 by hepatitis B virus X gene].
abstract: OBJECTIVE
  To determine whether hepatitis B virus X (HBX) protein expression affect the oval cells' response to anti-proliferative effect of transforming growth factor β1 (TGFβ1) in oval cells.
  
  
  METHODS
  Real-time PCR, Western blot analysis were performed to detect the expression of TGFβRII in HBX-transfected oval cells named HBX-EGFP-LE/6, and EGFP-LE/6, LE/6 control cells. In addition, exogenous TGFβ1 was added into all three oval cell lines, MTT assay was preformed to clarify different responses to the anti-proliferative effect of TGFβ1.
  
  
  RESULTS
  The TGFβRII mRNA levels in LE/6 and EGFP-LE/6 cells were (10.2 ± 1.8) and (8.8 ± 0.9) folds of those in HBX-EGFP-LE/6 cells, the difference was significant (P < 0.05). HBX protein expression also reduced the protein levels of TGFβRII in HBX-EGFP-LE/6 oval cells, compared to the control cells. The MTT results exhibited that, after TGFβ1 addition, proliferative inhibition rate in the HBX-EGFP-LE/6 cells was 18.1% ± 1.5% while those in control cells were 42.2% ± 2.8% and 41.9% ± 5.0%, the difference was significant (P < 0.01).
  
  
  CONCLUSIONS
  HBX protein expression affects TGFβRII transcriptional activity and protein synthesis, and insensitive oval cells to anti-proliferative effect of TGFβ1.
authors: Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
authors: Heng-yi
authors: HY
authors: Wang
authors: Hui-fang
authors: HF
authors: Liang
authors: Xiao-ping
authors: XP
authors: Chen
authors: Wei-peng
authors: WP
authors: Liu
authors: Zhan-guo
authors: ZG
authors: Zhang
authors: Zong-ping
authors: ZP
authors: Yu
authors: Chang-hai
authors: CH
authors: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D012333:RNA, Messenger; D015534:Trans-Activators; D053773:Transforming Growth Factor beta1; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein