title: In situ follicular lymphoma with progressive transformation of the germinal centers confirmed by laser capture microdissection, IGH gene rearrangement analysis, and fluorescence in situ hybridization for t(14;18).
abstract: The authors report an unusual case of in situ follicular lymphoma associated with progressive transformation of the germinal centers. The patient was a 74-year-old Chinese woman with sequential lymphadenopathy in the right and left cervical regions over a period of 2 months. The first biopsy revealed in situ follicular lymphoma with progressive transformation of germinal centers, and the biopsy of the second lymph node led to a diagnosis of in situ follicular lymphoma. The immunophenotype, polymerase chain reaction amplification of the immunoglobulin heavy chain gene, and fluorescence in situ hybridization for t(14;18) were analyzed in each biopsy specimen, which showed both specimens to have t(14;18)(q32;q21) and revealed progression from polyclonality to monoclonality. These findings suggest a case of multicentric in situ follicular lymphoma and provide new insights into the pathogenesis of this disease.
authors: Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Jin-nan
authors: JN
authors: Li
authors: Wen-yan
authors: WY
authors: Zhang
authors: Yuan
authors: Y
authors: Tang
authors: Gan-di
authors: GD
authors: Li
authors: Dan-dan
authors: DD
authors: Dong
mesh_terms: D000368:Aged; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000473:pathology*; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D002999:Clone Cells; D018450:Disease Progression; D005260:Female; D015321:Gene Rearrangement; D018858:Germinal Center / Q000473:pathology*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D061346:Laser Capture Microdissection; D008223:Lymphoma / Q000235:genetics / Q000473:pathology*; D008224:Lymphoma, Follicular / Q000235:genetics / Q000473:pathology*; D014178:Translocation, Genetic
chemical_list: D007143:Immunoglobulin Heavy Chains