title: Protective role of α2HS-glycoprotein in HBV-associated liver failure.
abstract: In this study, levels of plasma α2-Heremans-Schmid glycoprotein, serum tumor necrosis factor-α, serum liver function parameters and short-term mortality were measured in 100 hepatitis B patients. Release of interleukin-6 and tumor necrosis factor-α from the lipopolysaccharide-stimulated peripheral blood mononuclear cells in the presence/absence of spermine and α2-Heremans-Schmid glycoprotein were analyzed by enzyme-linked immunosorbent assay to determine the significance and potential mechanism of α2-Heremans-Schmid glycoprotein in hepatitis B virus-associated liver damage. Results showed that serum α2-Heremans-Schmid glycoprotein levels in acute-on-chronic liver failure patients were significantly lower than that in chronic hepatitis B patients or healthy controls (p < 0.05). A negative dependence between serum human α2-Heremans-Schmid glycoprotein and tumor necrosis factor-α levels was observed. Interleukin-6 and tumor necrosis factor-α levels in the lipopolysaccharide-induced peripheral blood mononuclear cell supernates were significantly reduced by spermine and/or α2-Heremans-Schmid glycoprotein. The latter two proteins jointly inhibited cytokine release. These observations suggest that plasma α2-Heremans-Schmid glycoprotein is an independent marker of liver damage and a prognostic indicator of hepatitis B virus chronicity. It may reduce liver inflammation by partially inhibiting release of inflammatory factors from activated peripheral blood mononuclear cells.
authors: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China; E-Mails: daixh0630@163.com (X.-H.D.); xmf427@126.com (M.-F.X.); rr_xy@hotmail.com (R.-R.Z.); zhangbx73@sina.com (B.-X.Z.); huguansheng@126.com (G.-S.H.); huangabing0330@yahoo.com.cn (Z.-B.H.).
authors: Xia-Hong
authors: XH
authors: Dai
authors: Pan
authors: P
authors: Zhang
authors: Mei-Fang
authors: MF
authors: Xiao
authors: Rong-Rong
authors: RR
authors: Zhou
authors: Bao-Xin
authors: BX
authors: Zhang
authors: Guan-Sheng
authors: GS
authors: Hu
authors: Ze-Bing
authors: ZB
authors: Huang
authors: Xue-Gong
authors: XG
authors: Fan
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006515:Hepatitis B virus / Q000502:physiology*; D019694:Hepatitis B, Chronic / Q000378:metabolism / Q000473:pathology; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D017093:Liver Failure / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D013096:Spermine / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D060749:alpha-2-HS-Glycoprotein / Q000032:analysis*
chemical_list: D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D060749:alpha-2-HS-Glycoprotein; D013096:Spermine