title: Effect of trypsin inhibitor activity in soya bean on growth performance, protein digestibility and incidence of sub-clinical necrotic enteritis in broiler chicken flocks.
abstract: 1. The effect of three different levels of dietary trypsin inhibitor activity (achieved by varying the amount of non-toasted full fat soya bean in replacement for toasted full fat soya bean) on the incidence of spontaneously-occurring sub-clinical necrotic enteritis (NE) in broiler chickens was compared. A fourth dietary treatment compared the effect of a diet that used potato protein concentrate as the major protein source. The determined trypsin inhibitor activity increased with the increasing content of non-toasted soya bean: 1·90, 6·21, 8·46 and 3·72 mg/g for the three soya bean diets (0, 100 and 200 g of non-toasted soya bean/kg) and the potato protein diet respectively. 2. Although increasing amounts of the non-toasted full-fat soya bean increased the feed intakes of the birds, there was a marked reduction in protein digestibility, weight gain and feed conversion efficiency. 3. There was a linear increase in sub-clinical NE lesions in the duodenum, jejunum, mid small intestine and ileum with increasing non-toasted soya bean. Caecal Clostridium perfringens counts increased with the increasing dietary content of non-toasted soya bean. Serum α-toxin antibodies were higher in the birds fed the 200 g non-toasted soya bean/kg diet compared with the other diets. 4. The results demonstrated that variation in the amount of non-toasted dietary soya bean not only affects growth performance of broilers but also affects the incidence of sub-clinical necrotic enteritis in the flock. Ensuring the lowest possible trypsin-inhibitor activity in soya bean samples is a valuable tool to improve the health and welfare of birds and in reducing the financial losses from this disease.
authors: National Institute of Poultry Husbandry, Harper Adams University College, Edgmond, Newport, Shropshire, England. mpalliyeguru@harper-adams.ac.uk
authors: M W C D
authors: MW
authors: Palliyeguru
authors: S P
authors: SP
authors: Rose
authors: A M
authors: AM
authors: Mackenzie
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D058345:Asymptomatic Infections / Q000453:epidemiology; D002645:Chickens / Q000254:growth & development / Q000502:physiology*; D004032:Diet / Q000662:veterinary; D004044:Dietary Proteins / Q000378:metabolism; D004751:Enteritis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D015994:Incidence; D007421:Intestine, Small / Q000473:pathology; D008297:Male; D009336:Necrosis / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary; D010940:Plant Proteins / Q000378:metabolism; D011201:Poultry Diseases / Q000453:epidemiology* / Q000473:pathology; D013025:Glycine max / Q000737:chemistry*; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins; D014361:Trypsin Inhibitors