title: Interleukin-18-mediated interferon-gamma secretion is regulated by thymosin beta 4 in human NK cells.
abstract: Thymosin beta 4 (Tβ4) is the major G-actin sequestering molecule and is abundant in lymphoid tissues. However, it is not clear what regulates Tβ4 expression and what its function is on natural killer (NK) cells. We investigated whether interleukin-18 (IL-18) has a role in Tβ4 expression and if enhanced Tβ4 influences IL-18-mediated interferon-gamma (IFN-γ) secretion. In this study, recombinant human IL-18 (rhIL-18) enhanced the endogenous level of Tβ4 through p38MAPK and JNK signaling pathway in the human NK cell line, NK-92MI. Overexpression of endogeneous Tβ4 stimulated IFN-γ expression and secretion. Additionally, pretreatment with an inhibitor for Tβ4 decreased IL-18-enhanced IFN-γ secretion, and transfection with Tβ4-specific short hairpin RNA resulted in reduction of IFN-γ production in primary NK cells as well as in the human NK cell line. Taken together, these data indicated that Tβ4 is regulated by IL-18 and is involved in IL-18-enhanced IFN-γ secretion in NK cells.
authors: Department of Life Science, Sookmyung Women's University, Seoul, Republic of Korea.
authors: Ha-reum
authors: HR
authors: Lee
authors: Sun Young
authors: SY
authors: Yoon
authors: Seok Bean
authors: SB
authors: Song
authors: Yoorim
authors: Y
authors: Park
authors: Tae Sung
authors: TS
authors: Kim
authors: Seonghan
authors: S
authors: Kim
authors: Dae Young
authors: DY
authors: Hur
authors: Hyun Keun
authors: HK
authors: Song
authors: Hyunjeong
authors: H
authors: Park
authors: Daeho
authors: D
authors: Cho
mesh_terms: D015870:Gene Expression / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D020382:Interleukin-18 / Q000378:metabolism* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D013947:Thymosin / Q000235:genetics / Q000378:metabolism*; D000099201:Thymosin beta(4)
chemical_list: D007371:Interferon-gamma; D020382:Interleukin-18; D013947:Thymosin; D000099201:Thymosin beta(4)