title: Interaction of lysozyme with gold nanorods: conformation and activity investigations.
abstract: Gold nanorods, with their unique morphology and optical properties have offered new prospects for biomedical and biosensing applications. Herein, the interaction between gold nanorods and a model protein has been monitored using spectroscopic techniques. The enzyme retains high fraction of its native structure with a slight increase in the helical content at the expense of β-turns. Kinetic investigations revealed notable increase of enzyme affinity for substrate without significant decrease in the V(max). Emission spectra of tryptophan residues in the presence of chaotropic agent highlighted the maintenance of internal quenching due to the induced compactness. Comparison of the gold nanorod treated lysozyme with free enzyme revealed higher thermodynamic stability under denaturing condition. Results from this study encourage the possibility of utilizing gold nanorods as promising nanocarrier candidates for a new generation of drug delivery applications.
authors: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors: Tahereh Tohidi
authors: TT
authors: Moghadam
authors: Bijan
authors: B
authors: Ranjbar
authors: Khosro
authors: K
authors: Khajeh
authors: Seyed Masoud
authors: SM
authors: Etezad
authors: Khosrow
authors: K
authors: Khalifeh
authors: Mohamad Reza
authors: MR
authors: Ganjalikhany
mesh_terms: D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D019791:Guanidine / Q000494:pharmacology; D007700:Kinetics; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D043942:Nanotubes / Q000737:chemistry*; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary; D058767:Protein Unfolding / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014364:Tryptophan / Q000378:metabolism
chemical_list: D006046:Gold; D014364:Tryptophan; D009113:Muramidase; D019791:Guanidine