title: Effects of amino acids infused into the vein on ghrelin-induced GH, insulin and glucagon secretion in lactating cows.
abstract: To investigate the effects of amino acids on ghrelin-induced growth hormone (GH), insulin and glucagon secretion in lactating dairy cattle, six Holstein cows were randomly assigned to two infusion treatments in a cross-over design. Mixture solution of amino acids (AMI) or saline (CON) was continuously infused into the left side jugular vein via catheter for 4 h. At 2 h after the start of infusion, synthetic bovine ghrelin was single injected into the right side jugular vein through the catheter. Ghrelin injection immediately increased plasma GH, glucose and non-esterified fatty acids (P<0.05) with no difference between both treatments. Additionally, plasma insulin and glucagon concentrations were increased by ghrelin injection in both treatments. The peak value of plasma insulin concentration was greater in AMI compared with CON (P<0.05). Plasma glucagon concentration showed no difference in the peak value reached at 5 min between both treatments, and then the plasma levels in AMI compared with CON showed sustained higher values (P<0.05). After plasma glucose concentration reached the peak, the decline was greater in AMI compared with CON (P<0.05). These results showed that the increased plasma amino acids may enhance ghrelin action which in turn enhances insulin and glucagon secretions in lactating cows.
authors: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors: Rika
authors: R
authors: Fukumori
authors: Akinori
authors: A
authors: Yokotani
authors: Toshihisa
authors: T
authors: Sugino
authors: Fumiaki
authors: F
authors: Itoh
authors: Shiro
authors: S
authors: Kushibiki
authors: Hiroyuki
authors: H
authors: Shingu
authors: Naoko
authors: N
authors: Moriya
authors: Yoshihisa
authors: Y
authors: Hasegawa
authors: Masayasu
authors: M
authors: Kojima
authors: Kenji
authors: K
authors: Kangawa
authors: Taketo
authors: T
authors: Obitsu
authors: Kohzo
authors: K
authors: Taniguchi
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000502:physiology*; D005260:Female; D054439:Ghrelin / Q000494:pharmacology*; D005934:Glucagon / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism*; D007262:Infusions, Intravenous; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007774:Lactation / Q000502:physiology; D011897:Random Allocation
chemical_list: D000596:Amino Acids; D054439:Ghrelin; D007328:Insulin; D013006:Growth Hormone; D005934:Glucagon