title: Reduction in miR-141 is induced by leukemia inhibitory factor and inhibits proliferation in choriocarcinoma cell line JEG-3.
abstract: OBJECTIVE
  Starting from the peri-implantation period, leukemia inhibitory factor (LIF) is a major regulator of trophoblast functions. Micro-RNAs (miRNA) are short non-coding RNA sequences, which regulate expression of genes at post-transcriptional level. The influence of LIF on miRNA expression in trophoblastic cells has not yet been analyzed and was focus of this investigation.
  
  
  METHODS
  JEG-3 choriocarcinoma cells have been stimulated with LIF for 1, 2, 4, 6, and 24 hr. The expression of miR-9, miR-141, miR-21, miR-93, and let-7 g has been analyzed by real-time PCR. Subsequently, miR-141 has been silenced and over-expressed to test its role in the proliferation of JEG-3 cells after 24 and 48 hr.
  
  
  RESULTS
  MiR-141 has been significantly downregulated by more than 50% after LIF stimulation, while miR-21 and miR-93 expression has been significantly upregulated. Silencing of miR-141 completely inhibited the proliferation of JEG-3 cells, while over-expression had no effect.
  
  
  CONCLUSIONS
  LIF regulates expression of miRNA in trophoblastic cells, which may be responsible for several functional effects induced by LIF.
authors: Klinik für Frauenheilkunde und Geburtshilfe, Abteilung für Geburtshilfe, Placenta-Labor, Friedrich-Schiller-Universität Jena, Jena, Germany.
authors: Diana M
authors: DM
authors: Morales-Prieto
authors: Ekkehard
authors: E
authors: Schleussner
authors: Udo R
authors: UR
authors: Markert
mesh_terms: D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D014327:Trophoblasts / Q000378:metabolism
chemical_list: D053605:Leukemia Inhibitory Factor; C533895:MIRN141 microRNA, human; D035683:MicroRNAs