title: Experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer by intracerebral route: final report.
abstract: Final observations on experimental transmission of chronic wasting disease (CWD) from elk (Cervus elaphus nelsoni) and white-tailed deer (Odocoileus virginianus) to fallow deer (Dama dama) are reported herein. During the 5-year study, 13 fawns were inoculated intracerebrally with CWD-infected brain material from white-tailed deer (n = 7; Group A) or elk (n = 6; Group B), and 3 other fawns were kept as uninoculated controls (Group C). As described previously, 3 CWD-inoculated deer were euthanized at 7.6 mo post-inoculation (MPI). None revealed presence of abnormal prion protein (PrP(d)) in their tissues. At 24 (Group A) and 26 (Group B) MPI, 2 deer were necropsied. Both animals had a small focal accumulation of PrP(d) in their midbrains. Between 29 and 37 MPI, 3 other deer (all from Group A) were euthanized. The 5 remaining deer became sick and were euthanized between 51 and 60 MPI (1 from Group A and 4 from Group B). Microscopic lesions of spongiform encephalopathy (SE) were observed in only these 5 animals; however, PrP(d) was detected in tissues of the central nervous system by immunohistochemistry, Western blot, and by commercial rapid test in all animals that survived beyond 24 MPI. This study demonstrates that intracerebrally inoculated fallow deer not only amplify CWD prions, but also develop lesions of spongiform encephalopathy.
authors: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, 1920 Dayton Avenue, PO Box 70, Ames, Iowa, USA. ahamir@mdanderson.org
authors: Amir N
authors: AN
authors: Hamir
authors: Justin J
authors: JJ
authors: Greenlee
authors: Eric M
authors: EM
authors: Nicholson
authors: Robert A
authors: RA
authors: Kunkle
authors: Juergen A
authors: JA
authors: Richt
authors: Janice M
authors: JM
authors: Miller
authors: Mark
authors: M
authors: Hall
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation / Q000662:veterinary*; D003670:Deer; D011328:Prions / Q000008:administration & dosage / Q000302:isolation & purification*; D034081:Wasting Disease, Chronic / Q000473:pathology / Q000635:transmission*
chemical_list: D011328:Prions