title: Primary stromal cells isolated from human various histological/pathological prostate have different phenotypes and tumor promotion role.
abstract: BACKGROUND
  Prostate stromal cells are known to regulate epithelial growth as well as support and maintain epithelial function. However, how stromal cells regulate epithelial cells and what differences among various histological/pathological prostate stromal cells in prostate cancer progression still remain unclear. This study aimed to investigate the different phenotypes of human various histological/pathological prostate stromal cells, and their role in tumor promotion.
  
  
  METHODS
  The different phenotypes of the human normal prostatic peripheral zonal primary stromal cells (NPPF), transitional zonal primary stromal cells (NPTF), and prostate cancer associated primary stromal cells (CAF) were examined with growth curves and Annexin V-fluorescein isothiocyanate (FITC) assay. The different effects on prostate cancer cell line C4-2B by NPPF, NPTF, and CAF were examined with MTT assay and Annexin V-FITC assay. The gene expression of different histological/pathological prostate stromal cells was profiled by microarray and hierarchical cluster analysis.
  
  
  RESULTS
  The growth rate of NPPF, NPTF and CAF gradually increased, followed by decreasing apoptosis. In vitro stromal-C4-2B cell line co-culture models, the proliferation and apoptosis of C4-2B cell line were differently affected by human various histological/pathological prostate stromal cells. CAF showed the most powerful effect to C4-2B cell line, as opposed to a weakest effect of NPTF. Microarray and hierarchical cluster analysis showed that the differentially expressed genes of CAF and NPPF were less than NPPF and NPTF, or CAF and NPTF. This was consistent with clinical observations that prostate cancer mostly derived from the peripheral zone and does not usually occur in the transitional zone.
  
  
  CONCLUSIONS
  NPPF, NPTF and CAF possess extremely different biological characteristics and gene expression, which may play an important role in genesis and development of prostate cancer.
authors: Department of Urology, First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China.
authors: Xiao-Hai
authors: XH
authors: Wang
authors: Fu-Jun
authors: FJ
authors: Zhao
authors: Bang-Min
authors: BM
authors: Han
authors: Qi
authors: Q
authors: Jiang
authors: Yong-Chuan
authors: YC
authors: Wang
authors: Jian-Hong
authors: JH
authors: Wu
authors: Yue-Qing
authors: YQ
authors: Tang
authors: Yue-Ping
authors: YP
authors: Zhang
authors: Shu-Jie
authors: SJ
authors: Xia
mesh_terms: D000328:Adult; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002478:Cells, Cultured; D016000:Cluster Analysis; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011467:Prostate / Q000166:cytology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism*; D014407:Tumor Cells, Cultured