title: Advances in microfluidic PCR for point-of-care infectious disease diagnostics.
abstract: Global burdens from existing or emerging infectious diseases emphasize the need for point-of-care (POC) diagnostics to enhance timely recognition and intervention. Molecular approaches based on PCR methods have made significant inroads by improving detection time and accuracy but are still largely hampered by resource-intensive processing in centralized laboratories, thereby precluding their routine bedside- or field-use. Microfluidic technologies have enabled miniaturization of PCR processes onto a chip device with potential benefits including speed, cost, portability, throughput, and automation. In this review, we provide an overview of recent advances in microfluidic PCR technologies and discuss practical issues and perspectives related to implementing them into infectious disease diagnostics.
authors: Department of Emergency Medicine, Johns Hopkins University, Baltimore, MD 21205, USA.
authors: Seungkyung
authors: S
authors: Park
authors: Yi
authors: Y
authors: Zhang
authors: Shin
authors: S
authors: Lin
authors: Tza-Huei
authors: TH
authors: Wang
authors: Samuel
authors: S
authors: Yang
mesh_terms: D003141:Communicable Diseases / Q000175:diagnosis*; D003906:Developing Countries; D014943:Global Health; D006801:Humans; D046210:Microfluidic Analytical Techniques; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction