title: Susceptibility of mosquito and lepidopteran cell lines to the mosquito iridescent virus (IIV-3) from Aedes taeniorhynchus.
abstract: Mosquito iridescent viruses (MIV) are members of the genus Chloriridovirus that currently contains only the type IIV-3 from Aedestaeniorhynchus. The complete genome of invertebrate iridescent virus -3 (IIV-3) has been sequenced and the availability of a tissue culture system would facilitate functional genomic studies. This investigation, using quantitative PCR and electron microscopy, has determined that the mosquito cell lines Aedes aegypti (Aag2), Aedes albopictus (C6/36) and Anopheles gambiae (4a3A) as well as the lepidopteran cell line from Spodoptera frugiperda (SF9) are permissive to IIV-3 infection. However, IIV-3 infection remained longer in Aag2 and C6/36 cells. Virus produced in C6/36 cell line was infectious to larvae of A. taeniorhynchus by injection and per os. Ultrastructural examination of 4a3A and SF9 cells infected with IIV-3 revealed an unusual feature, where virions were localized to mitochondria. It is speculated that containment with mitochondria may play a role in the lack of persistence in these cell lines.
authors: US Department of Agriculture, Agriculture Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL 32608, USA. James.Becnel@ars.usda.gov
authors: James J
authors: JJ
authors: Becnel
authors: Julia W
authors: JW
authors: Pridgeon
mesh_terms: D000330:Aedes / Q000166:cytology / Q000648:ultrastructure / Q000821:virology*; D000818:Animals; D000852:Anopheles / Q000166:cytology / Q000648:ultrastructure / Q000821:virology*; D002460:Cell Line; D007497:Iridoviridae / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D007814:Larva / Q000821:virology; D018411:Spodoptera / Q000166:cytology / Q000821:virology*