title: Liquid chromatography tandem mass spectrometry with ion trap and triple quadrupole analyzers for determination of thyreostatic drugs in urine and muscle tissue.
abstract: Liquid chromatography tandem mass spectrometry methods were developed and validated to screen for and confirm residues of the thyreostatic drugs: tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil in bovine and porcine urine and muscle tissues using dimethylthiouracil as internal standard. Thyreostats were extracted from urine samples with diethyl ether after derivatisation with 3-iodobenzylbromide in basic medium (pH 8.0) and analyzed by gradient elution on a Nucleosil C18 column with ion trap mass spectrometry detection using an electrospray source and triple quadrupole MS detection with turbo spray source. Thyreostats were extracted from muscle tissue with methanol, the denaturation of matrix protein was performed and then the same steps as for the urine samples were carried out. The methods were validated in accordance with the Commission Decision 2002/657/EC. Good thyreostats recoveries were obtained (from 82% to 117%) as well as acceptable within-lab reproducibility. The values of the decision limit CCα and the detection capability CCβ of five thyreostatic drugs are found to be below the recommended concentration set at 10 μg L(-1) (kg(-1)). The results of the validation demonstrate that liquid chromatography mass spectrometry with ion trap detection does not meet the criteria for confirmation for some thyreostats and therefore was applied for screening purpose only.
authors: Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland. bwozniak@piwet.pulawy.pl
authors: Barbara
authors: B
authors: Wozniak
authors: Iwona Matraszek
authors: IM
authors: Zuchowska
authors: Jan
authors: J
authors: Zmudzki
authors: Piotr
authors: P
authors: Jedziniak
authors: Beata
authors: B
authors: Korycinska
authors: Katarzyna
authors: K
authors: Sielska
authors: Sebastian
authors: S
authors: Witek
authors: Alicja
authors: A
authors: Klopot
mesh_terms: D000818:Animals; D013956:Antithyroid Agents / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D009132:Muscles / Q000737:chemistry; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D013956:Antithyroid Agents