title: Phosphodiesterase type 9 (PDE9) in the human lower urinary tract: an immunohistochemical study.
abstract: OBJECTIVE
  To examine the roles of the one of the cyclic guanosine monophosphate (cGMP)-specific phosphodiesterases (PDEs), PDE9, in human lower urinary tract (LUT), we performed immunohistochemistry (IHC) using autopsy specimens. To demonstrate the potential functional differences between PDE5 and PDE9 in human LUT, the localization of PDE5 and PDE9 was also compared using laser-capture microdissection (LCM)/reverse transcriptase-polymerase chain reaction (RT-PCR) method.
  
  
  METHODS
  •  We immunolocalized PDE9 in human bladder (60 cases) and prostate (40) specimens using IHC. We performed LCM/RT-PCR evaluation in six human bladders to determine the differential expression patterns of PDE5 and PDE9.
  
  
  RESULTS
  PDE9 immunoreactivity was detected in the bladder urothelium of all the cases and in 85% of the prostatic urethral urothelium. PDE9 immunoreactivity was detected in the perikaryon of the intramural ganglia. LCM/RT-PCR evaluation showed that PDE5 mRNA was exclusively detected in smooth muscle cells but PDE9 mRNA was mainly detected in the urothelium of the human bladder.
  
  
  CONCLUSIONS
  PDE9 is widely distributed in the urothelial epithelium of the human LUT and its potential roles may be different from those of PDE5.
authors: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors: Shuji
authors: S
authors: Nagasaki
authors: Youichi
authors: Y
authors: Nakano
authors: Mariko
authors: M
authors: Masuda
authors: Katsuhiko
authors: K
authors: Ono
authors: Yasuhiro
authors: Y
authors: Miki
authors: Yukiko
authors: Y
authors: Shibahara
authors: Hironobu
authors: H
authors: Sasano
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5 / Q000378:metabolism; D005260:Female; D005724:Ganglia / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D061346:Laser Capture Microdissection; D008297:Male; D008875:Middle Aged; D009130:Muscle, Smooth / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000235:genetics / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001743:Urinary Bladder / Q000378:metabolism*; D019459:Urothelium / Q000378:metabolism; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; C116137:PDE9A protein, human; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517381:PDE5A protein, human