title: PKCα take part in CCR7/NF-κB  autocrine signaling loop in CCR7-positive squamous cell carcinoma of head and neck.
abstract: Metastatic squamous cell carcinoma of head and neck (SCCHN) has been shown to express chemokine receptor 7 (CCR7). The role of nuclear factor-κB (NF-κB) in propagating an autocrine signaling loop in CCR7-positive SCCHN cells may provide a clinically useful biomarker of disease status and response to therapy. In this article, we hypothesized that PKCα might be involved in the CCR7/NF-κB autocrine signaling loop. Results showed that CCL19 induced the activation of PKCα, and the increased activity of PKCα was abolished by CCR7 mAb. PKCα inhibition with Gö6976 led to significant reduction in the activation and nuclear translocation of NF-κB induced by CCL19. Immunohistochemical assay also showed that CCR7 and PKCα were highly expressed in SCCHN and correlated with each other, which was significantly related to lymph node metastasis and clinical stage. Taken together, PKCα is involved in the CCR7/NF-κB autocrine signaling loop.
authors: Department of Orthodontics, School of Stomatology, China Medical University, No. 117 Nanjing Bei Jie, Heping District, Shenyang, Liaoning 110002, People's Republic of China.
authors: Zhao
authors: Z
authors: Zhen-jin
authors: Li
authors: L
authors: Peng
authors: Liu
authors: L
authors: Fa-yu
authors: Sun
authors: S
authors: Liyan
authors: Sun
authors: S
authors: Chang-fu
mesh_terms: D000328:Adult; D000368:Aged; D019898:Autocrine Communication / Q000235:genetics; D002227:Carbazoles / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D054415:Chemokine CCL19 / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D051571:Protein Kinase C-alpha / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D054400:Receptors, CCR7 / Q000235:genetics / Q000378:metabolism*
chemical_list: C515460:CCL19 protein, human; C515370:CCR7 protein, human; D002227:Carbazoles; D054415:Chemokine CCL19; D016328:NF-kappa B; D054400:Receptors, CCR7; C081021:Go 6976; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha