title: Genotypic dependent effect of exogenous glutathione on Cd-induced changes in proteins, ultrastructure and antioxidant defense enzymes in rice seedlings.
abstract: Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Xiushui63) and tolerant (Bing97252) rice genotypes to evaluate how different genotypes responded to Cd toxicity in presence of glutathione (GSH). Results showed that GSH alleviates Cd-toxicity, ameliorates Cd-induced damages on leaf/root ultrastructures. Nine proteins in roots were identified, using 2-DE coupled with mass spectrometry, whose expression were down-regulated in Xiushui63, up-regulated/unchanged in Bing97252 by Cd; coinstantaneously enhanced/unchanged in Cd+GSH over Cd alone treatment in both genotypes. They are l-ascorbate peroxidase, putative short-chain dehydrogenase/reductase, Glycolipid transfer protein, elongation factor, Os04g0652700, carbonic anhydrase, Os08g0374000, chitinase, and putative disease resistance response protein. Eight proteins in leaves with expression of increase in Bing97252 but down-regulate/unchange in Xiushui63, categorized as four groups of their functions: carbon metabolism, TCA cycle, photorespiration and RNA processing. Furthermore, we identified eight proteins with repressed expression in Cd-treated and up-regulated in Cd+GSH-treated rice leaves of Xiushui63.
authors: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
authors: Yue
authors: Y
authors: Cai
authors: Fangbin
authors: F
authors: Cao
authors: Kang
authors: K
authors: Wei
authors: Guoping
authors: G
authors: Zhang
authors: Feibo
authors: F
authors: Wu
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002104:Cadmium / Q000032:analysis*; D018506:Gene Expression Regulation, Plant; D005838:Genotype; D005978:Glutathione / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D012275:Oryza / Q000187:drug effects / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D011506:Proteins / Q000032:analysis*; D020543:Proteome; D040901:Proteomics / Q000379:methods; D012639:Seeds; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D006017:Glycolipids; D011506:Proteins; D020543:Proteome; D002104:Cadmium; D005978:Glutathione