title: [Enhancer activity of DNA fragments from FXYD5-COX7A region of human chromosome 19].
abstract: The enhancer activity of four previously identified within the one megabase region of human chromosome 19 DNA fragments was investigated. All four fragments had similar tissue-specific profile--maximum of enhancer activity was observed in HEK293 and minimum in HeLa cells. Enhancers obtained had pronounced specificity toward cytomegalovirus promoter compared with SV40 promoter. Functional dissection of one of the fragments (enhancer 14) demonstrated that only its inner 127 b.p. part possessed enhancer activity. The negative regulators, i.e. silencers or insulators are probably located in flanking regions of enhancer 14 and limit its effect on promoter. At the same time, enhancer activity of enhancer 14 depends on its orientation relative to promoter that isn't typical to majority of enhancer elements. Inner 127 b.p. fragment contains 11 transcription factor binding sites; 8 of them are factors that take part in immune system regulation. Enhancer 14 is located 500 b.p. upstream of transcription start site of TYROBP (DAP12) gene that codes for of T-killer cells activator protein and possibly functions as tissue-specific enhancer for this gene.
authors: A N
authors: AN
authors: Sorotokina
authors: I P
authors: IP
authors: Chernov
authors: E A
authors: EA
authors: Stukacheva
authors: L G
authors: LG
authors: Nikolaev
authors: E D
authors: ED
authors: Sverdlov
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D003587:Cytomegalovirus / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D058507:Host Specificity; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D007473:Ion Channels; D008562:Membrane Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D009928:Organ Specificity; D055607:Receptors, Natural Killer Cell / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C451410:FXYD5 protein, human; D007473:Ion Channels; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D055607:Receptors, Natural Killer Cell; C110546:TYROBP protein, human; D004247:DNA; C513901:COX7A1 protein, human; D003576:Electron Transport Complex IV