title: Cervical precancerous lesions--chromosomal instability in peripheral blood lymphocytes in relation to lesion stage, age and smoking habits.
abstract: OBJECTIVE
  To evaluate chromosomal damage in peripheral blood lymphocytes (PBL) of patients newly diagnosed with cervical precancerous lesions with respect to age, smoking habits, miscarriages, abortions and lesion stage.
  
  
  METHODS
  Clinical study.
  
  
  METHODS
  Clinic of Gynecology and Obstetrics in Kragujevac, Serbia, during 2009-2010.
  
  
  METHODS
  The analyzed samples included 41 untreated patients aged 24-65 years with a diagnosed low-grade squamous intraepithelial lesion (LSIL; 19 patients) or a high-grade squamous intraepithelial lesion (HSIL; 22 patients). Control samples were obtained from 40 healthy women aged 24-53 years.
  
  
  METHODS
  The frequency of micronuclei (MN) was estimated in circulating lymphocytes by using the cytokinesis-block micronucleus assay.
  
  
  METHODS
  The frequency of MN in PBL.
  
  
  RESULTS
  The mean MN frequencies of both LSIL and HSIL patients were significantly higher than the MN frequency of healthy control women. There was no significant difference in MN frequency between LSIL and HSIL patients, between smokers and nonsmokers in both patient and control samples, or between miscarriage groups and abortion groups of patients. Considering confounder factors, age and health status influenced MN frequency.
  
  
  CONCLUSIONS
  The results suggest that MN frequency in PBL of patients with cervical precancerous lesions corresponds to an increase of chromosomal damage, irrespective of smoking habits, miscarriages, abortions and lesion stages.
authors: Faculty of Science, University of Kragujevac, Radoja Domanovíca12, Kragujevac, Serbia. olivera@kg.ac.rs
authors: Olivera
authors: O
authors: Milošević-Djordjević
authors: Ivana
authors: I
authors: Stošić
authors: Darko
authors: D
authors: Grujičić
authors: Dragić
authors: D
authors: Banković
authors: Slobodan
authors: S
authors: Arsenijević
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000704:Analysis of Variance; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology*; D016022:Case-Control Studies; D043171:Chromosomal Instability; D005260:Female; D006801:Humans; D015994:Incidence; D016014:Linear Models; D008214:Lymphocytes / Q000473:pathology; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011230:Precancerous Conditions / Q000473:pathology*; D011379:Prognosis; D012016:Reference Values; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000473:pathology; D055815:Young Adult