title: Proteomic analysis of the interleukin-4 (IL-4) response in hepatitis B virus-positive human hepatocelluar carcinoma cell line HepG2.2.15.
abstract: Hepatitis B virus (HBV) infection is the leading cause of liver cirrhosis and hepatocellular carcinoma worldwide. In recent decades, significant progress toward understanding the molecular virology and pathogenesis of HBV infection has been made. In addition, multiple treatment modalities have been developed for persons with HBV infection. In the present study, we demonstrated that IL-4 inhibits the expression of hepatitis B surface antigen and hepatitis B e antigen in a HBV stably transfected hepatocellular carcinoma cell line (HepG2.2.15). To reveal the anti-HBV mechanism of IL-4 by proteomics, 2-DE and MS technology were utilized to profile global changes in protein expression in HepG2.2.15 cells after IL-4 treatment. A total of 56 differentially expressed proteins were identified in IL-4-treated HepG2.2.15 cells. To find out the interaction of these changed proteins by bioinformatics, signaling network analysis with the STRING tool showed that the identified proteins are primarily involved in transcription and proteolysis. Taken together, these results offer valuable clues for understanding the molecular mechanisms of the IL-4-mediated anti-HBV response.
authors: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, P R China.
authors: Yuqin
authors: Y
authors: Yao
authors: Jiong
authors: J
authors: Li
authors: Zejun
authors: Z
authors: Lu
authors: Aiping
authors: A
authors: Tong
authors: Wei
authors: W
authors: Wang
authors: Xiaolan
authors: X
authors: Su
authors: Yan
authors: Y
authors: Zhou
authors: Bo
authors: B
authors: Mu
authors: Shijie
authors: S
authors: Zhou
authors: Xiaoan
authors: X
authors: Li
authors: Lijuan
authors: L
authors: Chen
authors: Lantu
authors: L
authors: Gou
authors: Hongbing
authors: H
authors: Song
authors: Jinliang
authors: J
authors: Yang
authors: Yuquan
authors: Y
authors: Wei
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D006509:Hepatitis B / Q000188:drug therapy / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D006513:Hepatitis B e Antigens / Q000096:biosynthesis; D006515:Hepatitis B virus / Q000276:immunology*; D054884:Host-Pathogen Interactions; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D015847:Interleukin-4