title: A role for nyctalopin, a small leucine-rich repeat protein, in localizing the TRP melastatin 1 channel to retinal depolarizing bipolar cell dendrites.
abstract: Expression of channels to specific neuronal sites can critically impact their function and regulation. Currently, the molecular mechanisms underlying this targeting and intracellular trafficking of transient receptor potential (TRP) channels remain poorly understood, and identifying proteins involved in these processes will provide insight into underlying mechanisms. Vision is dependent on the normal function of retinal depolarizing bipolar cells (DBCs), which couple a metabotropic glutamate receptor 6 to the TRP melastatin 1 (TRPM1) channel to transmit signals from photoreceptors. We report that the extracellular membrane-attached protein nyctalopin is required for the normal expression of TRPM1 on the dendrites of DBCs in mus musculus. Biochemical and genetic data indicate that nyctalopin and TRPM1 interact directly, suggesting that nyctalopin is acting as an accessory TRP channel subunit critical for proper channel localization to the synapse.
authors: Department of Biochemistry and Molecular Biology, University of Louisville, Louisville, Kentucky 40202, USA.
authors: Jillian N
authors: JN
authors: Pearring
authors: Pasano
authors: P
authors: Bojang
authors: Yin
authors: Y
authors: Shen
authors: Chieko
authors: C
authors: Koike
authors: Takahisa
authors: T
authors: Furukawa
authors: Scott
authors: S
authors: Nawy
authors: Ronald G
authors: RG
authors: Gregg
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001703:Biophysics; D002461:Cell Line, Transformed; D003712:Dendrites / Q000378:metabolism*; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D000090263:Leucine-Rich Repeat Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008954:Models, Biological; D018408:Patch-Clamp Techniques; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology* / Q000254:growth & development; D051245:Retinal Bipolar Cells / Q000166:cytology*; D050053:TRPM Cation Channels / Q000172:deficiency / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D014966:Xanthenes / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D018691:Excitatory Amino Acid Antagonists; C114624:LY 341495; D000090263:Leucine-Rich Repeat Proteins; D008164:Luminescent Proteins; D011506:Proteins; D011509:Proteoglycans; D050053:TRPM Cation Channels; C116297:Trpm1 protein, mouse; D014966:Xanthenes; C560879:nyctalopin protein, mouse; C054562:yellow fluorescent protein, Bacteria