title: Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography.
abstract: A phenylboronate affinity monolith was prepared and applied to the selective capture of glycoproteins from unfractionated protein mixtures. The monolith was synthesized in a 100 μm i.d capillary by an in situ polymerization procedure using a pre-polymerization mixture consisting of 4-vinylphenylboronic acid (VPBA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, diethylene glycol and ethylene glycol as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The prepared monolith was characterized in terms of the morphology, pore property, and recognition property. The selectivity and dynamic binding capacity were evaluated by using standard glycoproteins and nonglycoproteins as model proteins. The chromatographic results demonstrated that the phenylboronate affinity monolith had higher selectivity and binding capacity for glycoprotein than nonglycoprotein. The resulting phenylboronate affinity monolith was used as the sorbent for in-tube solid phase microextraction (in-tube SPME), and the extraction performance of the monolith was assessed by capture of ovalbumin from egg white sample.
authors: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China. zianlin@fzu.edu.cn
authors: Zi An
authors: ZA
authors: Lin
authors: Ji Lei
authors: JL
authors: Pang
authors: Yao
authors: Y
authors: Lin
authors: Hui
authors: H
authors: Huang
authors: Zong Wei
authors: ZW
authors: Cai
authors: Lan
authors: L
authors: Zhang
authors: Guo Nan
authors: GN
authors: Chen
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry*; D002645:Chickens / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D008689:Methacrylates / Q000737:chemistry; D010047:Ovalbumin / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: C427523:4-vinylphenyl boronic acid; D001897:Boronic Acids; D008689:Methacrylates; D011108:Polymers; D014753:Vinyl Compounds; C004919:ethylene dimethacrylate; D010047:Ovalbumin