title: Antibodies to lytic infection proteins in lymphocryptovirus-infected rhesus macaques: a model for humoral immune responses to epstein-barr virus infection.
abstract: Humoral immune responses to rhesus lymphocryptovirus (rhLCV) lytic infection proteins were evaluated in the rhesus macaque animal model for Epstein-Barr virus (EBV) infection. We found a hierarchy of humoral responses to 14 rhLCV lytic infection proteins in naturally infected rhesus macaques, with (i) widespread and robust responses to four glycoproteins expressed as late proteins, (ii) frequent but less robust responses to a subset of early proteins, and (iii) low-level responses to immediate-early proteins. This hierarchy of humoral responses was similar to that reported for EBV-infected humans, with the notable exception of the response to rhBARF1. Serum antibodies to rhBARF1 were frequently detected in healthy rhLCV-infected macaques, but in humans, anti-BARF1 antibodies have been reported primarily in patients with EBV-positive nasopharyngeal carcinoma (NPC). The macaque data accurately predicted that serum antibodies against BARF1 are a normal response to EBV infection when human serum samples are analyzed. The rhesus macaque animal provides a unique perspective on humoral responses to EBV infection in humans and can be a valuable model for EBV vaccine development.
authors: Channing Laboratory, 181 Longwood Avenue, Boston, MA 02115, USA.
authors: Nina
authors: N
authors: Orlova
authors: Mark H
authors: MH
authors: Fogg
authors: Angela
authors: A
authors: Carville
authors: Fred
authors: F
authors: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002277:Carcinoma; D004195:Disease Models, Animal; D020031:Epstein-Barr Virus Infections / Q000276:immunology* / Q000821:virology; D006566:Herpesviridae Infections / Q000276:immunology / Q000821:virology; D006801:Humans; D018143:Lymphocryptovirus / Q000276:immunology* / Q000472:pathogenicity; D008253:Macaca mulatta / Q000276:immunology* / Q000821:virology; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000276:immunology / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C086495:BARF1 protein, Human herpesvirus 4; D011994:Recombinant Proteins; D014764:Viral Proteins