title: Comparison of neoeriocitrin and naringin on proliferation and osteogenic differentiation in MC3T3-E1.
abstract: Naringin is considered the main effective compound of Drynaria Rhizome, which is used commonly in the treatment of osteoporosis in traditional Chinese medicine. However, we found neoeriocitrin, a new compound isolated from Drynaria Rhizome, showed a better activity than naringin on proliferation and osteogenic differentiation in MC3T3-E1. Both neoeriocitrin and naringin exhibited the best effect on proliferation and osteogenic differentiation at concentration of 2μg/ml. Neoeriocitrin more significantly improved proliferation and alkaline phosphatase (ALP) activity as well as up-regulated Runx2, COLI and OCN expression by 56%, 37% and 14% respectively than naringin. Furthermore, neoeriocitrin could rescue the inhibition effect of cell differentiation induced by PD98059 to some degree. Therefore, neoeriocitrin may be a new promising candidate drug for treatment of osteoporosis.
authors: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 10084, PR China.
authors: Lina
authors: L
authors: Li
authors: Zhen
authors: Z
authors: Zeng
authors: Guoping
authors: G
authors: Cai
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004187:Disaccharides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology; D015870:Gene Expression; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D029621:Polypodiaceae / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D004187:Disaccharides; D044950:Flavanones; D005419:Flavonoids; D012333:RNA, Messenger; C553460:neoeriocitrin; D000469:Alkaline Phosphatase; C005274:naringin; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one