title: Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences.
abstract: BACKGROUND
  Ischaemia-reperfusion is one of the main causes of kidney complications. The most frequent lesion is acute tubular necrosis. Ozone oxidative preconditioning exerts a modulatory effect of redox state of renal cells in models of ischaemia-reperfusion, by stimulating endogenous antioxidant mechanisms. Similar results have been obtained in more recent studies using ischaemic postconditioning.
  
  
  OBJECTIVE
  To evaluate the effect of ozone oxidative postconditioning on renal function and morphology in an ischaemia-reperfusion rat model.
  
  
  METHODS
  We used forty female Wistar rats weighing between 150g-200g randomly divided into 4 groups (negative control, positive control, oxygen and ozone). The groups: positive control, oxygen and ozone were subjected to 60 minutes of ischaemia and 10 days of reperfusion. During reperfusion, the oxygen group was given 26mg/kg body weight of oxygen, and the ozone group 0.5mg/kg body weight of ozone, rectally. At the end of the experiment urine and blood samples were taken for renal function tests and kidneys were removed for histological study.
  
  
  RESULTS
  The ozone group showed no significant differences for filtration fraction and proteinuria compared to the negative control group. The glomerular filtrate rate, renal plasma flow and creatinine showed a slight improvement in comparison with oxygen and positive control groups. The ozone group showed significantly less overall histological damage than the positive control and oxygen groups.
  
  
  CONCLUSIONS
  Ozone postconditioning showed to have a protective effect in preserving renal function and morphology.
authors: Departamento de Investigaciones, Instituto de Nefrología Dr. Abelardo Buch, Ave. 26 y Ave. Rancho Boyeros, La Habana, Cuba. aimeefdez@infomed.sld.cu
authors: A
authors: A
authors: Fernández Iglesias
authors: L
authors: L
authors: González Núñez
authors: J L
authors: JL
authors: Calunga Fernández
authors: S
authors: S
authors: Rodríguez Salgueiro
authors: E
authors: E
authors: Santos Febles
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D004334:Drug Administration Schedule; D004353:Drug Evaluation, Preclinical; D005260:Female; D005919:Glomerular Filtration Rate; D007511:Ischemia / Q000188:drug therapy*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D007683:Kidney Tubular Necrosis, Acute / Q000188:drug therapy / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D008297:Male; D010100:Oxygen / Q000008:administration & dosage / Q000627:therapeutic use; D010126:Ozone / Q000008:administration & dosage / Q000627:therapeutic use*; D011507:Proteinuria / Q000209:etiology / Q000517:prevention & control; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D010126:Ozone; D010100:Oxygen