title: Over-expression of chloroperoxidase in Caldariomyces fumago.
abstract: The filamentous fungus Caldariomyces fumago secretes a chloroperoxidase (CPO). To increase its production, we integrated a CPO-expression cassette into the non-transcribed spacer regions of the rDNA in C. fumago. One strain was obtained that had twice the CPO activity when grown in shake-flask and bioreactor compared to the wild-type. The highest CPO activity from the bioreactor cultivation was 3,236 U ml(-1). This is the highest value reported so far.
authors: DECHEMA eV, Biochemical Engineering, Frankfurt am Main, Germany. buchhaupt@dechema.de
authors: Markus
authors: M
authors: Buchhaupt
authors: Kristina
authors: K
authors: Ehrich
authors: Sonja
authors: S
authors: Hüttmann
authors: Jan
authors: J
authors: Guder
authors: Jens
authors: J
authors: Schrader
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics*; D019149:Bioreactors; D002711:Chloride Peroxidase / Q000235:genetics* / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D015870:Gene Expression; D011995:Recombination, Genetic
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D002711:Chloride Peroxidase