title: Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans.
abstract: When Methanosarcina                         acetivorans grows on carbon monoxide (CO), it synthesizes high levels of a protein, MA4079, homologous to aldehyde dehydrogenases. To investigate the role of MA4079 in M. acetivorans, mutants lacking the encoding gene were generated and phenotypically analyzed. Loss of MA4079 had no effect on methylotrophic growth but led to complete abrogation of methylotrophic growth in the presence of even small amounts of CO, which indicated the mutant's inability to acclimate to the presence of this toxic gas. Prolonged incubation with CO allowed the isolation of a strain in which the effect of MA4079 deletion is suppressed. The strain, designated Mu3, tolerated the presence of high CO partial pressures even better than the wild type. Immunological analysis using antisera against MA4079 suggested that it is not abundant in M. acetivorans. Comparison of proteins differentially abundant in Mu3 and the wild type revealed an elevated level of methyl-coenzyme M reductase and a decreased level of one isoform of carbon monoxide dehydrogenase/acetyl-coenzyme A synthase, which suggests that pleiotropic mutation(s) compensating for the loss of MA4079 affected catabolism. The data presented point toward a role of MA4079 to enable M. acetivorans to properly acclimate to CO.
authors: Institut für Molekulare Biowissenschaften, Molekulare Mikrobiologie und Bioenergetik, Johann Wolfgang Goethe-Universität Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.
authors: Michael
authors: M
authors: Kliefoth
authors: Julian D
authors: JD
authors: Langer
authors: Nicole
authors: N
authors: Matschiavelli
authors: Ellen
authors: E
authors: Oelgeschläger
authors: Michael
authors: M
authors: Rother
mesh_terms: D000064:Acclimatization / Q000235:genetics; D000444:Aldehyde Dehydrogenase / Q000235:genetics* / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology; D002248:Carbon Monoxide / Q000378:metabolism / Q000494:pharmacology; D017020:Methanosarcina / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D009097:Multienzyme Complexes / Q000378:metabolism; D009154:Mutation; D010088:Oxidoreductases / Q000378:metabolism; D020543:Proteome / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D020543:Proteome; D002245:Carbon Dioxide; D002248:Carbon Monoxide; D010088:Oxidoreductases; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; C019823:carbon monoxide dehydrogenase; C021572:methyl coenzyme M reductase