title: Effect of six tropical tanniferous plant extracts on larval exsheathment of Haemonchus contortus.
abstract: Tanniferous plants represent a promising alternative for controlling gastrointestinal nematodes of small ruminants. This experiment evaluated the effects of extracts from the leaf and stem of Anadenanthera colubrina, Leucaena leucocephala and Mimosa tenuiflora on larval exsheathment of Haemonchus contortus in vitro and verified the role of tannins in this process. Third-stage larvae of H. contortus were incubated with extracts for 3 hours and were exposed to sodium hypochlorite solution. The extracts were tested at 300 μg.mL(-1) and accompanied by controls: phosphate buffer solution (PBS) and polyvinyl polypyrrolidone (PVPP). The larval exsheathment was evaluated for 60 minutes, and the results were subjected to the Kruskal-Wallis test (p < 0.05). The six extracts blocked larval exsheathment. After PVPP addition, a tannin inhibitor, the exsheathment percentage was similar to the PBS (p > 0.05), except for L. leucocephala and M. tenuiflora leaf extracts. However, pre-incubation with PVPP of these two extracts significantly changed larval exsheathment when compared to the non-treated extracts (p < 0.05). These results suggest that A. colubrina, L. leucocephala and M. tenuiflora could be useful in gastrointestinal nematode control and that tannins are probably the main compounds involved in the observed effects. However, in vivo and toxicological studies should be conducted.
authors: Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Av. Dedé Brazil 1700, Campus do Itaperi, Fortaleza, CE, Brazil.
authors: Lorena Mayana Beserra de
authors: LM
authors: Oliveira
authors: Claudia Maria Leal
authors: CM
authors: Bevilaqua
authors: Iara Tersia Freitas
authors: IT
authors: Macedo
authors: Selene Maia de
authors: SM
authors: Morais
authors: Maria Vivina Barros
authors: MV
authors: Monteiro
authors: Claudio Cabral
authors: CC
authors: Campello
authors: Wesley Lyeverton Correia
authors: WL
authors: Ribeiro
authors: Emanuelle Karine Frota
authors: EK
authors: Batista
mesh_terms: D000818:Animals; D006189:Haemonchus / Q000187:drug effects*; D007814:Larva / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D013634:Tannins / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D013634:Tannins