title: Diversity of bacterial communities related to the nitrogen cycle in a coastal tropical bay.
abstract: A culture-independent molecular phylogenetic analysis was carried out to study for the first time the diversity of bacterial ammonia monooxygenase subunit A (amoA) and nitrogenase reductase subunit H (nifH) genes from Urca inlet at Guanabara Bay in Rio de Janeiro, Brazil. Most bacterial amoA and nifH sequences exhibited identities of less than 95% to those in the GenBank database revealing that novel ammonia-oxidizing bacteria and nitrogen-fixing microorganisms may exist in this tropical marine environment. The observation of a large number of clones related to uncultured bacteria also indicates the necessity to describe these microorganisms and to develop new cultivation methodologies.
authors: Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil.
authors: Alessandra M
authors: AM
authors: Gonzalez
authors: Ricardo P
authors: RP
authors: Vieira
authors: Alexander M
authors: AM
authors: Cardoso
authors: Maysa M
authors: MM
authors: Clementino
authors: Rodolpho M
authors: RM
authors: Albano
authors: Leda
authors: L
authors: Mendonça-Hagler
authors: Orlando B
authors: OB
authors: Martins
authors: Rodolfo
authors: R
authors: Paranhos
mesh_terms: D001419:Bacteria / Q000201:enzymology / Q000235:genetics*; D061006:Bays / Q000382:microbiology*; D001938:Brazil; D017753:Ecosystem; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D016013:Likelihood Functions; D058458:Nitrogen Cycle / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D014329:Tropical Climate
chemical_list: D010088:Oxidoreductases; C029130:nitrogenase reductase; C045582:ammonia monooxygenase