title: Proteomic analysis of secretory products from the model gastrointestinal nematode Heligmosomoides polygyrus reveals dominance of venom allergen-like (VAL) proteins.
abstract: The intestinal helminth parasite, Heligmosomoides polygyrus bakeri offers a tractable experimental model for human hookworm infections such as Ancylostoma duodenale and veterinary parasites such as Haemonchus contortus. Parasite excretory-secretory (ES) products represent the major focus for immunological and biochemical analyses, and contain immunomodulatory molecules responsible for nematode immune evasion. In a proteomic analysis of adult H. polygyrus secretions (termed HES) matched to an extensive transcriptomic dataset, we identified 374 HES proteins by LC-MS/MS, which were distinct from those in somatic extract HEx, comprising 446 identified proteins, confirming selective export of ES proteins. The predominant secreted protein families were proteases (astacins and other metalloproteases, aspartic, cysteine and serine-type proteases), lysozymes, apyrases and acetylcholinesterases. The most abundant products were members of the highly divergent venom allergen-like (VAL) family, related to Ancylostoma secreted protein (ASP); 25 homologues were identified, with VAL-1 and -2 also shown to be associated with the parasite surface. The dominance of VAL proteins is similar to profiles reported for Ancylostoma and Haemonchus ES products. Overall, this study shows that the secretions of H. polygyrus closely parallel those of clinically important GI nematodes, confirming the value of this parasite as a model of helminth infection.
authors: Institute of Immunology and Infection Research, University of Edinburgh, Edinburgh EH9 3JT, UK.
authors: James P
authors: JP
authors: Hewitson
authors: Yvonne
authors: Y
authors: Harcus
authors: Janice
authors: J
authors: Murray
authors: Maaike
authors: M
authors: van Agtmaal
authors: Kara J
authors: KJ
authors: Filbey
authors: John R
authors: JR
authors: Grainger
authors: Stephen
authors: S
authors: Bridgett
authors: Mark L
authors: ML
authors: Blaxter
authors: Peter D
authors: PD
authors: Ashton
authors: David A
authors: DA
authors: Ashford
authors: Rachel S
authors: RS
authors: Curwen
authors: R Alan
authors: RA
authors: Wilson
authors: Adam A
authors: AA
authors: Dowle
authors: Rick M
authors: RM
authors: Maizels
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D004195:Disease Models, Animal; D005767:Gastrointestinal Diseases / Q000469:parasitology*; D015801:Helminth Proteins / Q000032:analysis* / Q000378:metabolism; D009351:Nematospiroides dubius / Q000737:chemistry*; D040901:Proteomics / Q000379:methods
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins