title: Cytotoxicity of 45S5 bioglass paste used for dentine hypersensitivity treatment.
abstract: OBJECTIVE
  45S5 bioglass mixed with 50% phosphoric acid has been suggested to treat dentine hypersensitivity and incipient enamel caries. This study is going to evaluate the biocompatibility of using the aforementioned technique with the rat pulpal cells.
  
  
  METHODS
  The relative cytotoxicity of 45S5 bioglass on rat dental pulp cells was compared to the cytotoxicity of a temporary filling material (Caviton; GC, Japan), Type 1 glass ionomer cement (Fuji I; GC, Tokyo, Japan) and commercial desensitising agent (SuperSeal; Phoenix Dental, Fenton, MI, USA) using a transwell insert model. Cell viability was measured by means of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The number of viable cell counts were compared using one way ANOVA (p<0.05). The morphological alterations of the pulp cells were observed directly by phase contrast microscope.
  
  
  RESULTS
  The results of this study indicated that cell viability recorded by the 45S5 bioglass paste group did not differ significantly from those of the Caviton, glass ionomer or superseal, moreover pulpal cells microscopic analysis revealed that 45S5 bioglass elicited minimal toxic effect.
  
  
  CONCLUSIONS
  45S5 bioglass paste can serve as a biocompatible material that can potentially be used safely on dentine.
authors: Cariology and Operative Dentistry Department, Graduate school of Medical and Dental Sciences, Tokyo Medical and Dental University, Japan. drahmedbakry@gmail.com
authors: Ahmed Samir
authors: AS
authors: Bakry
authors: Yukihiko
authors: Y
authors: Tamura
authors: Masayuki
authors: M
authors: Otsuki
authors: Shohei
authors: S
authors: Kasugai
authors: Keiichi
authors: K
authors: Ohya
authors: Junji
authors: J
authors: Tagami
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002133:Calcium Sulfate / Q000737:chemistry; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000633:toxicity*; D004396:Coloring Agents; D003738:Dental Cements / Q000737:chemistry; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D057133:Dentin Desensitizing Agents / Q000633:toxicity*; D005898:Glass / Q000737:chemistry; D005899:Glass Ionomer Cements / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D019815:Oxalic Acid / Q000737:chemistry; D010756:Phosphoric Acids / Q000633:toxicity; D051381:Rats; D012387:Root Canal Filling Materials / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D014753:Vinyl Compounds / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; D003738:Dental Cements; D057133:Dentin Desensitizing Agents; D005899:Glass Ionomer Cements; C510984:NovaMin; D010756:Phosphoric Acids; D012387:Root Canal Filling Materials; C474198:SuperSeal; D013778:Tetrazolium Salts; D013844:Thiazoles; D014753:Vinyl Compounds; C425330:bioactive glass 45S5; C086076:calcium sulfate, zinc oxide, vinyl acetate, zinc phosphate root canal filling; C084541:Fuji glass-ionomer lining cement; D019815:Oxalic Acid; C030242:phosphoric acid; C022616:thiazolyl blue; D015034:Zinc Oxide; D002133:Calcium Sulfate