title: Susceptibility of the cactus weevil Metamasius spinolae to Beauveria bassiana and Metarhizium anisopliae under laboratory and field conditions.
abstract: OBJECTIVE
  To evaluate different entomopathogenic fungal isolates against the cactus weevil Metamasius spinolae under laboratory and field conditions, and select an isolate to be used as a tool in the management of this insect pest.
  
  
  RESULTS
  Four experiments were carried out. The effect of temperature on the in vitro growth of eight isolates of entomopathogenic fungi (two Metarhizium anisopliae and six Beauveria bassiana) was assessed. The susceptibility of adult M. spinolae to the same isolates was evaluated. Using three selected isolates, the interaction between susceptibility and sex of the insect was studied. Finally, a field experiment was carried out to evaluate infection of adult M. spinolae by the same three isolates under natural abiotic conditions. Overall, growth rate was greatest at 25°C for all the isolates. In vitro growth of M. anisopliae was greater than B. bassiana. Mortality of adult M. spinolae was greater when inoculated with B. bassiana compared with isolates of M. anisopliae. Susceptibility had no interaction with the sex of the insect. The proportion of insects succumbing to infection was smaller when incubated under field conditions than when incubated under laboratory conditions.
  
  
  CONCLUSIONS
  The experiments described here showed a complex interaction between entomopathogenic fungi and M. spinolae, and these data allows us to select isolate Bb107 as a first step towards its use in the management of this pest insect.
  
  
  CONCLUSIONS
  Our results showed that entomopathogenic fungi can be used for the control of M. spinolae, which may help reduce the use of chemical insecticides and, therefore, the exposure of Opuntia ficus-indica producers to pesticides.
authors: Especialidad de Entomología y Acarología, Colegio de Postgraduados, Campus Montecillo, Texcoco, Estado de Mexico, Mexico.
authors: N
authors: N
authors: Orduño-Cruz
authors: A W
authors: AW
authors: Guzmán-Franco
authors: E
authors: E
authors: Rodríguez-Leyva
authors: J
authors: J
authors: López-Collado
authors: J M
authors: JM
authors: Valdéz-Carrasco
authors: G
authors: G
authors: Mora-Aguilera
mesh_terms: D000818:Animals; D052982:Beauveria / Q000254:growth & development / Q000472:pathogenicity*; D029421:Cactaceae / Q000469:parasitology*; D005260:Female; D008297:Male; D052981:Metarhizium / Q000254:growth & development / Q000472:pathogenicity*; D010572:Pest Control, Biological / Q000379:methods*; D013696:Temperature; D048889:Weevils / Q000382:microbiology*