title: In vivo iron quantification in collagenase-induced microbleeds in rat brain.
abstract: Brain microbleeds (BMB) are associated with chronic and acute cerebrovascular disease. Because BMB present in the brain is a source of potentially cytotoxic iron proportional to the volume of extravasated blood, BMB iron content is a potentially valuable biomarker both to assess tissue risk and small cerebral vessel health. We recently reported methods to quantify focal iron sources using phase images that were tested in phantoms and BMB in postmortem tissue. In this study, we applied our methods to small hemorrhagic lesions induced in the in vivo rat brain using bacterial collagenase. As expected by theory, measurements of geometric features in phase images correlated with lesion iron content measured by graphite furnace atomic absorption spectrometry. Iron content estimation following BMB in an in vivo rodent model could shed light on the role and temporal evolution of iron-mediated tissue damage and efficacy of potential treatments in cerebrovascular diseases associated with BMB.
authors: Neurosurgery Center for Research, Training and Education, Loma Linda University, Loma Linda, California 92354, USA.
authors: Grant
authors: G
authors: McAuley
authors: Matthew
authors: M
authors: Schrag
authors: Samuel
authors: S
authors: Barnes
authors: Andre
authors: A
authors: Obenaus
authors: April
authors: A
authors: Dickson
authors: Wolff
authors: W
authors: Kirsch
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D017364:Collagenases; D007091:Image Processing, Computer-Assisted; D020300:Intracranial Hemorrhages / Q000378:metabolism*; D007501:Iron / Q000032:analysis*; D016014:Linear Models; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic
chemical_list: D015415:Biomarkers; D007501:Iron; D017364:Collagenases