title: Time course investigation of intervertebral disc degeneration produced by needle-stab injury of the rat caudal spine: laboratory investigation.
abstract: OBJECTIVE
  In this study, the authors' goal was to investigate the long-term progression of disc degeneration and the participating mechanisms induced by needle puncture in a rat caudal disc model.
  
  
  METHODS
  The C5-6 and C7-8 intervertebral discs of the caudal spine in rats were stabbed laterally using 21-gauge hypodermic needles to a depth of 5 mm from the subcutaneous surface with the aid of fluoroscopy. Signs of degeneration in the disc of the tail were analyzed from Day 1 to Week 30 by in vivo MR imaging, histology, and biochemical and/or molecular analyses.
  
  
  RESULTS
  Magnetic resonance imaging showed a progressive decrease in T2 density and MR imaging index throughout the entire investigation, starting at Day 1 after the needle puncture. However, histological scores revealed a bimodal pattern, showing that severity increased in the first 17 days, declined thereafter, and increased again by 30 weeks. Gene expression analysis showed a transient up-regulation in gene expression of aggrecan, type II collagen, and BMP-2, and inhibition of type I collagen. The MMP-3 mRNA levels were up-regulated at all tested time points within 6 weeks postinjury. Furthermore, the degenerated disc did not recover spontaneously, as shown by decreases in T2 density, MR imaging index, and sulfated glycosaminoglycan content in conjunction with increases in histological scores at 15 and 30 weeks postsurgery.
  
  
  CONCLUSIONS
  This study demonstrates that needle puncture into a tail disc in the rat induces a rapid and progressive disc degeneration process without spontaneous recovery. Changes in gene expression profiles of the disc matrix molecules as well as anabolic and catabolic factors at early time points further delineate the mechanism of disc degeneration in this newly developed animal model.
authors: Spine Research Laboratory, Department of Neurosurgery, University of Michigan, Ann Arbor, Michigan, USA.
authors: Huina
authors: H
authors: Zhang
authors: Sushan
authors: S
authors: Yang
authors: Lin
authors: L
authors: Wang
authors: Paul
authors: P
authors: Park
authors: Frank
authors: F
authors: La Marca
authors: Scott J
authors: SJ
authors: Hollister
authors: Chia-Ying
authors: CY
authors: Lin
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D007403:Intervertebral Disc / Q000378:metabolism / Q000473:pathology*; D055959:Intervertebral Disc Degeneration / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013122:Spinal Diseases / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D053674:Aggrecans; D055396:Bone Morphogenetic Protein 2; D024043:Collagen Type II