title: Effect of San'ao Decoction on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma.
abstract: OBJECTIVE
  San'ao Decoction (, SAD), as a representative Chinese medicine (CM) formula, was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide (LPS) enhanced asthma model.
  
  
  METHODS
  The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin (OVA), challenged by OVA and LPS. After Balb/C mice's administration of a dose (0.0024 g/kg) of dexamethasone acetate, and three doses (2.2 g/kg, 4.4 g/kg and 8.8 g/kg) of SAD, airway inflammation and responsiveness were observed. The airway inflammation was detected by counting bronchoalveolar lavage fluid (BALF) cells and lung histopathology. Also, differential expressions of interferon-r (IFN-γ), interleukin-4 (IL-4), and IL-5 in the supernatants of BALF were examined. The changes in airway responsiveness indicated by lung resistance (R(L)) and stimulated by acetylcholine (Ach) were determined.
  
  
  RESULTS
  Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma, while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness, and to some extent, reduced airway inflammation. Meanwhile, the small-dose, medium-dose, and high-dose SAD promoted Th1-type cytokines (IFN-γ) and reduced Th2-type cytokines (IL-4, IL-5) to different extents, which led to a Th1/Th2 balance.
  
  
  CONCLUSIONS
  SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model, but its definite influence on airway inflammation is not remarkable.
authors: Department of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors: Peng-Cheng
authors: PC
authors: Gu
authors: Xin-Sheng
authors: XS
authors: Fan
authors: Chen-Xue
authors: CX
authors: Jiang
authors: Hui-Qin
authors: HQ
authors: Xu
authors: Jing-Hua
authors: JH
authors: Yu
authors: Yu-Ping
authors: YP
authors: Tang
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology*; D016535:Bronchial Hyperreactivity / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011014:Pneumonia / Q000150:complications* / Q000188:drug therapy* / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; D015848:Interleukin-5; D008070:Lipopolysaccharides; C546519:San-Ao decoction; D015847:Interleukin-4; D007371:Interferon-gamma