title: Carbon tetrachloride: a hepatotoxin causes oxidative stress in murine peritoneal macrophage and peripheral blood lymphocyte cells.
abstract: BACKGROUND
  Carbon tetrachloride (CCl₄) is frequently used as a chemical inducer of tissue damage. Their effects on mouse peritoneal macrophages and also in peripheral blood lymphocytes are still unknown.
  
  
  OBJECTIVE
  Therefore we tried to focus on intracellular oxidative stress produced by CCl₄ in mouse macrophage and lymphocyte cells.
  
  
  METHODS
  Intraperitoneal administration of CCl₄ induces intracellular superoxide anions production in mouse macrophages and peripheral blood lymphocytes and leads a subsequent lipid peroxidation and protein oxidation. N-acetyl cystein (NAC) and vitamin C were administered intraperitoneally at a dose of 150 mg/kg and their effect on demodulating the oxidative stress is also checked.
  
  
  CONCLUSIONS
  Several in vitro approaches have already been established as a free radical scavenging models, but this free radical screening models is not always correlated with the in vivo screening models. NAC and vitamin C were administered intraperitoneally and significant reduction of the oxidative stress in term of scavenging of toxic superoxide anion observed in both the macrophages and lymphocytes.
  
  
  CONCLUSIONS
  Therefore we are hopeful that our work will light a new insight into the screening of in vivo free radical scavenging model for evaluating anti-inflammatory compounds.
authors: Division of Pharmacology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata,West Bengal, India. asisbala_ju@yahoo.co.in
authors: Asis
authors: A
authors: Bala
authors: Pallab Kanti
authors: PK
authors: Haldar
authors: Biswakanth
authors: B
authors: Kar
authors: Sagar
authors: S
authors: Naskar
authors: Upal Kanti
authors: UK
authors: Mazumder
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism* / Q000473:pathology; D016166:Free Radical Scavengers / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D013481:Superoxides; D002251:Carbon Tetrachloride; D001205:Ascorbic Acid; D000111:Acetylcysteine