title: Cladribine enhances apoptotic cell death in lung carcinoma cells over-expressing DNase γ.
abstract: Worldwide, lung cancer is the most common form of cancer and often has a poor prognosis. Establishment of effective therapies for lung cancer is a major concern in clinical cancer research. We compared the cytotoxic effects of chemotherapeutic agents including cisplatin, 5-fluorouracil, vinorelbine and cladribine, on a human lung cancer cell line, A549, and its derivative transfected with the DNase γ gene. We observed selective cytotoxicity of cladribine on the DNase γ-expressing sub-cell line of A549. Cladribine induces selective apoptosis in DNase γ-expressing A549 cells, which depends on activation of caspases. These results suggest that a combination therapy that includes cladribine along with the introduction of DNase γ has potential as a new therapeutic strategy for lung cancer.
authors: Faculty of Science and Engineering, Tokyo University of Science, 1–1–1 Daigaku-dori, Yamaguchi, Yamaguchi 756–0884, Japan.
authors: Akemi
authors: A
authors: Hayakawa
authors: Haruhiko
authors: H
authors: Suzuki
authors: Yuzuru
authors: Y
authors: Kamei
authors: Sei-ichi
authors: S
authors: Tanuma
authors: Junji
authors: J
authors: Magae
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D017338:Cladribine / Q000494:pharmacology*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology*
chemical_list: D017338:Cladribine; D004706:Endodeoxyribonucleases; C105124:deoxyribonuclease gamma