title: Differentiation of human LAN-5 neuroblastoma cells induced by extremely low frequency electronically transmitted retinoic acid.
abstract: BACKGROUND
  Jaques Benveniste suggested that electronic transmission of 4-phorbol-12-b-myristate-13-acetate (PMA) activity, carried out using a simple amplifier configured to function as an oscillator when coupled to two electromagnetic coils, demonstrates the same chemical activity as the active molecule. The results obtained suggested that there are associated signals at the PMA molecules that can be transferred to target neutrophils by artificial physical means in a fashion that mimics the original molecules.
  
  
  METHODS
  Retinoic acid was placed at room temperature on one coil attached to an oscillator (VEGA select 719), while LAN-5 neuroblastoma cells were placed on another coil and incubated under controlled condition. The oscillator was then turned on for 12 hours a day for 5 days, after which cells were counted and morphology studied by contrast microscopy.
  
  
  RESULTS
  The effect of the differentiating agent added to the cell culture by physical means generates a decrease in cell growth, metabolic activity, and the protrusion of a neuritelike structure typical of the differentiated cells.
  
  
  CONCLUSIONS
  These preliminary results suggest that retinoic acid molecules emit signals that can be transferred to LAN-5 neuroblastoma cells by artificial physical means in a manner that seems related to the chemical structure of the source molecules.
authors: Bititalia, Milan, Italy.
authors: Alberto
authors: A
authors: Foletti
authors: Mario
authors: M
authors: Ledda
authors: Enrico
authors: E
authors: D'Emilia
authors: Settimio
authors: S
authors: Grimaldi
authors: Antonella
authors: A
authors: Lisi
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D004581:Electronics / Q000379:methods*; D006801:Humans; D016501:Neurites; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D014212:Tretinoin / Q000737:chemistry
chemical_list: D014212:Tretinoin