title: Differential distribution of blood-derived proteins in xenografted human adenocarcinoma tissues by in vivo cryotechnique and cryobiopsy.
abstract: Tumor behavior depends on the complex tumor interstitium and microenvironment, which influence transport of fluid and soluble molecules from blood vessels. The purpose of this study was to reveal how complex tumor tissues affect the immunodistribution of serum proteins and time-dependent translocation of bovine serum albumin (BSA) from blood vessels, using relatively differentiated human adenocarcinoma produced by the xenografted A549 cell line. Histological architecture and immunodistribution of the serum proteins in adenocarcinomatous tissues were clearly detected by the in vivo cryotechnique and cryobiopsy. Both albumin and IgG1 were detected in blood vessels, connective tissues around the tumor mass, and the interstitium among tumor cell nests. IgM was mainly detected in blood vessels and connective tissues around the tumor mass but was not detected in the interstitium among the tumor cell nests. At 10 or 30 min after BSA injection, BSA was observed only in blood vessels, but 1 h after the injection, it was also detected in the interstitium and surrounding connective tissues of the tumor mass. The present findings showed topographic variation of molecular permeation in the adenocarcinomatous tumor mass. The interstitial tissues with augmented permeability of serum proteins would increase accessibility of tumor cells to blood-derived molecules.
authors: Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi, 409-3898, Japan.
authors: Yuqin
authors: Y
authors: Bai
authors: Nobuhiko
authors: N
authors: Ohno
authors: Nobuo
authors: N
authors: Terada
authors: Sei
authors: S
authors: Saitoh
authors: Tadao
authors: T
authors: Nakazawa
authors: Nobuki
authors: N
authors: Nakamura
authors: Ryohei
authors: R
authors: Katoh
authors: Shinichi
authors: S
authors: Ohno
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D001706:Biopsy / Q000379:methods*; D001808:Blood Vessels / Q000473:pathology*; D002417:Cattle; D045744:Cell Line, Tumor; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000378:metabolism; D007075:Immunoglobulin M / Q000032:analysis / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D016612:Paraffin Embedding; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000032:analysis / Q000378:metabolism; D014018:Tissue Distribution; D016707:Tissue Fixation / Q000379:methods*; D014183:Transplantation, Heterologous / Q000379:methods
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012710:Serum Albumin, Bovine