title: Interleukin-33 induces expression of adhesion molecules and inflammatory activation in human endothelial cells and in human atherosclerotic plaques.
abstract: OBJECTIVE
  Interleukin (IL)-33 is the most recently described member of the IL-1 family of cytokines and it is a ligand of the ST2 receptor. While the effects of IL-33 on the immune system have been extensively studied, the properties of this cytokine in the cardiovascular system are much less investigated. Methods/Results- We show here that IL-33 promoted the adhesion of human leukocytes to monolayers of human endothelial cells and robustly increased vascular cell adhesion molecule-1, intercellular adhesion molecule-1, endothelial selectin, and monocyte chemoattractant protein-1 protein production and mRNA expression in human coronary artery and human umbilical vein endothelial cells in vitro as well as in human explanted atherosclerotic plaques ex vivo. ST2-fusion protein, but not IL-1 receptor antagonist, abolished these effects. IL-33 induced translocation of nuclear factor-κB p50 and p65 subunits to the nucleus in human coronary artery endothelial cells and human umbilical vein endothelial cells and overexpression of dominant negative form of IκB kinase 2 or IκBα in human umbilical vein endothelial cells abolished IL-33-induced adhesion molecules and monocyte chemoattractant protein-1 mRNA expression. We detected IL-33 and ST2 on both protein and mRNA level in human carotid atherosclerotic plaques.
  
  
  CONCLUSIONS
  We hypothesize that IL-33 may contribute to early events in endothelial activation characteristic for the development of atherosclerotic lesions in the vessel wall, by promoting adhesion molecules and proinflammatory cytokine expression in the endothelium.
authors: Department of Internal Medicine II, Medical University of Vienna, Vienna, Austria.
authors: Svitlana
authors: S
authors: Demyanets
authors: Viktoria
authors: V
authors: Konya
authors: Stefan P
authors: SP
authors: Kastl
authors: Christoph
authors: C
authors: Kaun
authors: Sabine
authors: S
authors: Rauscher
authors: Alexander
authors: A
authors: Niessner
authors: Richard
authors: R
authors: Pentz
authors: Stefan
authors: S
authors: Pfaffenberger
authors: Kathrin
authors: K
authors: Rychli
authors: Christof E
authors: CE
authors: Lemberger
authors: Rainer
authors: R
authors: de Martin
authors: Akos
authors: A
authors: Heinemann
authors: Ihor
authors: I
authors: Huk
authors: Marion
authors: M
authors: Gröger
authors: Gerald
authors: G
authors: Maurer
authors: Kurt
authors: K
authors: Huber
authors: Johann
authors: J
authors: Wojta
mesh_terms: D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000209:etiology*; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology; D016328:NF-kappa B / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D058226:Plaque, Atherosclerotic / Q000209:etiology*; D011956:Receptors, Cell Surface / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; C079241:IL1RL1 protein, human; C506536:IL33 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins; D016328:NF-kappa B; D011956:Receptors, Cell Surface; D019869:Phosphatidylinositol 3-Kinases