title: Exploring the reactions of β-amyloid (Aβ) peptide 1-28 with Al(III) and Fe(III) ions.
abstract: The reactions of human β-amyloid peptide 1-28 (Aβ28) with Al(III) and Fe(III) ions were investigated by (1)H NMR and electrospray ionization mass spectrometry (ESI-MS) under pH conditions close to physiological ones. (1)H NMR titrations, performed in the 5.3-8.0 pH range, revealed that no measurable amounts of Aβ28-Al(III) or Aβ28-Fe(III) adducts are formed; such metal adducts could not be obtained even by changing a number of experimental conditions, e.g., temperature, buffer, nature of the salt, etc. These observations were later confirmed by ESI-MS. It is thus demonstrated that Aβ28, at physiological pH, is not able to form binary complexes with Al(III) and Fe(III) ions of sufficient stability to compete with metal hydroxide precipitation. The biological implications of these findings are discussed in the frame of current literature.
authors: Department of Chemistry, University of Siena, Via A. Moro, 53100 Siena, Italy. valensindan@unisi.it
authors: Daniela
authors: D
authors: Valensin
authors: Caterina
authors: C
authors: Migliorini
authors: Gianni
authors: G
authors: Valensin
authors: Elena
authors: E
authors: Gaggelli
authors: Giovanni
authors: G
authors: La Penna
authors: Henryk
authors: H
authors: Kozlowski
authors: Chiara
authors: C
authors: Gabbiani
authors: Luigi
authors: L
authors: Messori
mesh_terms: D000535:Aluminum / Q000737:chemistry* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C084358:amyloid beta-protein (1-28); D000535:Aluminum; D007501:Iron