title: Plant lipid transfer protein allergens: no cross-reactivity between those from foods and olive and Parietaria pollen.
abstract: BACKGROUND
  Cross-reactivity among plant food allergens belonging to the nonspecific lipid transfer protein (LTP) family is well known. In contrast, the relationship among these allergens and their putative homologs from olive (Ole e 7) and Parietaria (Par j 1) pollen has not been clarified.
  
  
  METHODS
  Sera with specific IgE to LTP allergens were obtained from peach-, mustard- and olive pollen-allergic patients. Purified LTP allergens from foods (peach, apple, mustard and wheat) and pollens (olive, mugwort and Parietaria) were tested by ELISA and ELISA-inhibition assays.
  
  
  RESULTS
  Plant food LTP-allergic patients showed a significantly higher number of sera (89-100 vs. 33-64%) with specific IgE and mean specific IgE levels (0.30-1.56 vs. 0.21-0.34 OD units) to the 4 food LTP allergens tested than to olive Ole e 7 and Parietaria Par j 1 pollen. ELISA-inhibition assays indicated cross-inhibition between food LTP allergens but no cross-reactivity between these allergens and Ole e 7 and Par j 1, or, even more, between the LTP allergens from olive and Parietaria pollen.
  
  
  CONCLUSIONS
  LTP allergens from olive and Parietaria pollen cross-react neither with allergenic LTPs from plant foods nor between themselves. Therefore, both pollens do not seem to be related with the LTP syndrome.
authors: Unidad de Bioquímica, Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, UPM, Madrid, España.
authors: Leticia
authors: L
authors: Tordesillas
authors: Sofía
authors: S
authors: Sirvent
authors: Araceli
authors: A
authors: Díaz-Perales
authors: Mayte
authors: M
authors: Villalba
authors: Javier
authors: J
authors: Cuesta-Herranz
authors: Rosalía
authors: R
authors: Rodríguez
authors: Gabriel
authors: G
authors: Salcedo
mesh_terms: D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D003429:Cross Reactions; D005512:Food Hypersensitivity; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D031658:Olea / Q000276:immunology; D032403:Parietaria / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D016415:Sequence Alignment
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D002352:Carrier Proteins; C107504:OLE6 protein, Olea europaea; C105336:PMAI protein, Parietaria judaica; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; D007073:Immunoglobulin E