title: Rapid detection of lily symptomless virus with CdTe quantum dots by flow cytometry.
abstract: Lily symptomless virus (LSV) is the most common lily virus, being detected in many species and hybrids. We established a microsphere-based fluorescent immunoassay for the determination of LSV, using a polyclonal antibody against LSV covalently bound to carboxy-modified microspheres able to capture LSV antigen. A monoclonal antibody against LSV conjugated to quantum dots (QDs) was used as a fluorescent probe, enabling LSV to be fluorescently detected by a combination of encoded beads and QDs. This method was 16 times more sensitive than ELISA in the detection of LSV, and could potentially be applied to the simultaneous detection of inhomogeneous matter.
authors: Chinese Academy of Inspection and Quarantine, Beijing, PR China.
authors: Mingqiang
authors: M
authors: Zou
authors: Fan
authors: F
authors: Zhang
authors: Jinfeng
authors: J
authors: Li
authors: Nan
authors: N
authors: Wang
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry*; D017788:Carlavirus / Q000302:isolation & purification* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D027762:Lilium / Q000821:virology*; D008863:Microspheres; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D013691:Tellurium; C028337:cadmium telluride