title: High-throughput quantification of hydroxyproline for determination of collagen.
abstract: An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prior to assay is what limits the current method for determining hydroxyproline. This work describes the conditions of alkali hydrolysis that, when combined with the colorimetric assay defined by Woessner, provide a high-throughput, accurate method for the measurement of hydroxyproline.
authors: Natural Extracts Unit, The New Zealand Institute for Plant & Food Research Limited, Port Nelson, Nelson 7043, New Zealand. Kathleen.Hofman@plantandfood.co.nz
authors: Kathleen
authors: K
authors: Hofman
authors: Bronwyn
authors: B
authors: Hall
authors: Helen
authors: H
authors: Cleaver
authors: Susan
authors: S
authors: Marshall
mesh_terms: D000468:Alkalies / Q000737:chemistry; D000818:Animals; D003094:Collagen / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D005399:Fishes; D057166:High-Throughput Screening Assays; D006868:Hydrolysis; D006909:Hydroxyproline / Q000032:analysis*; D012867:Skin / Q000737:chemistry
chemical_list: D000468:Alkalies; D003094:Collagen; D006909:Hydroxyproline