title: Modulation of fibroblast inflammatory response by surface modification of a perfluorinated ionomer.
abstract: An ideal surface for implantable glucose sensors would be able to evade the events leading to chronic inflammation and fibrosis, thereby extending its utility in an in vivo environment. Nafion™, a perfluorinated ionomer, is the membrane material preferred for in situ glucose sensors. Unfortunately, the surface properties of Nafion™ promote random protein adsorption and eventual foreign body encapsulation, thus leading to loss of glucose signal over time. Details of the techniques to render Nafion™ nonprotein fouling are given in a previous article [T. I. Valdes et al., Biomaterials 29, 1356 (2008)]. Once random protein adsorption is prevented, a biologically active peptide can be covalently bonded to the treated Nafion™ to induce cellular adhesion. Cellular responses to these novel decorated Nafion™ surfaces are detailed here, including cell viability, cell spreading, and type I collagen synthesis. Normal human dermal fibroblasts (NHDFs) were cultured on control and modified Nafion™ surfaces. Findings indicate that Nafion™ modified with 10% 2-hydroxyethyl methacrylate and 90% tetraglyme created a nonfouling surface that was subsequently decorated with the YRGDS peptide. NHDFs were shown to have exhibited decreased type I collagen production in comparison to NHDF cells on unmodified Nafion™ surfaces. Here, the authors report evidence that proves that optimizing conditions to prevent protein adsorption and enhance cellular adhesion may eliminate fibrous encapsulation of an implant.
authors: Center for Biomaterials, University of Connecticut Health Center, Farmington, Connecticut 06030-1715, USA.
authors: Thelma I
authors: TI
authors: Valdes
authors: Winston
authors: W
authors: Ciridon
authors: Buddy D
authors: BD
authors: Ratner
authors: James D
authors: JD
authors: Bryers
mesh_terms: D015374:Biosensing Techniques; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000009:adverse effects*; D003094:Collagen / Q000378:metabolism; D005026:Ethylene Glycols / Q000378:metabolism; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D008689:Methacrylates / Q000378:metabolism; D019736:Prostheses and Implants / Q000009:adverse effects*
chemical_list: D020099:Coated Materials, Biocompatible; D005026:Ethylene Glycols; D008689:Methacrylates; C005044:hydroxyethyl methacrylate; C441631:tetraglyme; D003094:Collagen