title: Enhancement of bioconversion efficiency of limonin by Pseudmonas putida G7.
abstract: The biocatalytic activity of periplasmic dehydrogenase of Pseudomonas putida G7 strain to catalyse limonin was enhanced when whole cells were permeabilized with EDTA (1 μM) lysozyme (100 μg/ml). The treated cells were entrapped in dialysis membranes to increase the stability. Permeabilized cells (1 g dry weight) entrapped in dialysis membrane could biotransform 73.67% of limonin in unpasteurized mandarin juices in a single-batch cycle of 3 h. Furthermore, permeabilized cells stored for 45 days in phosphate buffered saline (at 4 or 30°C) retained enzyme activity and were reusable for up to eight batch cycles of limonin reduction. The results of this study suggest a potential application of permeabilized P. putida G7 cells for reducing limonin levels in mandarin juices.
authors: Department of Biotechnology and Environmental Sciences, Thapar University, Patiala-147004, Punjab, India.
authors: Meenakshi
authors: M
authors: Malik
authors: Abhijit
authors: A
authors: Ganguli
authors: Moushumi
authors: M
authors: Ghosh
mesh_terms: D001711:Biotransformation; D002384:Catalysis; D002957:Citrus / Q000737:chemistry*; D003956:Dialysis; D005638:Fruit / Q000737:chemistry*; D036701:Limonins / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D010539:Permeability; D028321:Plant Preparations / Q000737:chemistry; D016958:Pseudomonas putida / Q000166:cytology / Q000201:enzymology*
chemical_list: D036701:Limonins; D028321:Plant Preparations; D010088:Oxidoreductases; C001546:limonin