title: Isolation and characterization of a fungus able to degrade pyrethroids and 3-phenoxybenzaldehyde.
abstract: Fungal strain HU, isolated from activated sludge and identified as a member of the genus Cladosporium based on morphology and sequencing of 28S rRNA, was shown to degrade 90% of fenvalerate, fenpropathrin, β-cypermethrin, deltamethrin, bifenthrin, and permethrin (100 mgL(-1)) within 5 days. Fenvalerate was utilized as sole carbon and energy source and co-metabolized in the presence of sucrose. Degradation of fenvalerate occurred at pH 5-10 at 18-38°C. The fungus first hydrolyzed the carboxylester linkage to produce α-hydroxy-3-phenoxy-benzeneacetonitrile and 3-phenoxybenzaldehyde, and subsequently degraded these two compounds with a q(max), K(s) and K(i) of 1.73 d(-1), 99.20 mgL(-1) and 449.75 mgL(-1), respectively. Degradation followed first-order kinetics. These results show that the fungal strain may possess potential to be used in bioremediation of pyrethroid-contaminated environments.
authors: Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, PR China.
authors: Shaohua
authors: S
authors: Chen
authors: Qiongbo
authors: Q
authors: Hu
authors: Meiying
authors: M
authors: Hu
authors: Jianjun
authors: J
authors: Luo
authors: Qunfang
authors: Q
authors: Weng
authors: Kaiping
authors: K
authors: Lai
mesh_terms: D001483:Base Sequence; D001547:Benzaldehydes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D017931:DNA Primers; D005658:Fungi / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009570:Nitriles / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011722:Pyrethrins / Q000378:metabolism*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*
chemical_list: D001547:Benzaldehydes; D017931:DNA Primers; D009570:Nitriles; D011722:Pyrethrins; D012339:RNA, Ribosomal, 28S; C064808:3-phenoxybenzaldehyde; C017690:fenvalerate