title: Batatasin I, a naturally occurring phenanthrene derivative, isolated from tuberous roots of Dioscorea batatas suppresses eicosanoids generation and degranulation in bone marrow derived-mast cells.
abstract: To find anti-inflammatory compounds from the tuberous roots of Dioscorea batatas, we isolated 6-hydroxy-2,4,7-trimethoxyphenanthrene (batatasin I) from the dichloromethane (CH(2)Cl(2)) fraction of this plant. Batatasin I inhibited both the generation of prostaglandin D(2) (PGD(2)), leukotriene C(4) (LTC(4)) and degranulation reaction in mouse bone marrow-derived mast cells (BMMCs). This compound inhibited cyclooxygenase-2 (COX-2) dependent PGD(2) generation in a dose dependent manner, with IC(50) values of 1.78 µM. Western blotting probed with specific anti-COX-2 antibodies showed that the decrease in the quantity of the PGD(2) generation was accompanied by a decrease in the COX-2 protein level. In addition, this compound also inhibited the production of 5-lipoxygenase (5-LOX) dependent LTC(4) in a dose dependent manner (IC(50), 1.56 µM). Batatasin I also inhibited the mast cell degranulation reaction (IC(50), 6.7 µM) in BMMCs. This result indicates that batatasin I could be developed as an anti-inflammatory agent through further investigation.
authors: College of Pharmacy, Yeungnam University, Gyeongsan 712–749, Republic of Korea.
authors: Yue
authors: Y
authors: Lu
authors: Meihua
authors: M
authors: Jin
authors: Sang-Jo
authors: SJ
authors: Park
authors: Kun Ho
authors: KH
authors: Son
authors: Jong-Keun
authors: JK
authors: Son
authors: Hyeun Wook
authors: HW
authors: Chang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects*; D015550:Cell Degranulation / Q000187:drug effects*; D027742:Dioscorea / Q000737:chemistry*; D015777:Eicosanoids / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D015289:Leukotrienes / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: C568298:6-hydroxy-2,4,7-trimethoxyphenanthrene; D000893:Anti-Inflammatory Agents; D015777:Eicosanoids; D015289:Leukotrienes; D010616:Phenanthrenes