title: Human leukocyte antigen-G expression on dendritic cells induced by transforming growth factor-beta1 and CD4+ T cells proliferation.
abstract: During antigen capture and processing, mature dendritic cells (DC) express large amounts of peptide-MHC complexes and accessory molecules on their surface. DC are antigen-presenting cells that have an important role in tolerance and autoimmunity. The transforming growth factor-beta1 (TGF-Beta1) cytokine has a regulatory role on the immune and non-immune cells. The aim of this study is to evaluate the effect of TGF-Beta1 on the induction of human leukocyte antigen-G (HLA-G) expression on the DC which is derived from monocyte.
  
  
  
  In this study, we evaluated the effect of TGF-Beta1 in induction HLA-G expression on the monocyte-derived DC by flowcytometry and then CD4+ T cell proliferative responses in the presence of DC-treated TGF-Beta1 was studied.
  
  
  
  The results of this study showed that DC bearing HLA-G down-regulated activation of CD4+ T cells and production of IL-6 and IL-17 in comparison with control (P<0.05).
  
  
  
  It is concluded that TGF-Beta1 has an important regulatory role in CD4+ T cell proliferation by increasing HLA-G on DC and these cells can probably prevent unexpected immune responses in vivo.
authors: Dept. of Microbiology and Immunology, Mazandaran University of Medical Sciences, Sari, Iran.
authors: Saeid
authors: S
authors: Abediankenari
authors: Dept. of Pathology, Faculty of Medicine, Mazandaran 
  University of Medical Sciences, Sari, Iran.
authors: Maryam
authors: M
authors: Ghasemi
authors: Dept. of Microbiology and Immunology, 
  Walter Oncology Center, Indianapolis, Indiana, USA.
authors: Young-June
authors: YJ
authors: Kim
mesh_terms: D051940:B7-2 Antigen / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D059951:HLA-G Antigens / Q000276:immunology*; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D010641:Phenotype; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D051940:B7-2 Antigen; D006684:HLA-DR Antigens; D059951:HLA-G Antigens; D020381:Interleukin-17; D015850:Interleukin-6; D008070:Lipopolysaccharides; D053773:Transforming Growth Factor beta1