title: Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing.
abstract: Heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing were utilised to genotype human parvovirus B19 samples from Brazil and Paraguay. Ninety-seven serum samples were collected from individuals presenting with abortion or erythema infectiosum, arthropathies, severe anaemia and transient aplastic crisis; two additional skin samples were collected by biopsy. After the procedure, all clinical samples were classified as genotype 1.
authors: Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Brasil.
authors: Marcos César Lima de
authors: MC
authors: Mendonça
authors: Ana Maria de Amorim
authors: AM
authors: Ferreira
authors: Marta Gonçalves Matos dos
authors: MG
authors: Santos
authors: Elva Cristina
authors: EC
authors: Oviedo
authors: Maria Sônia Dal
authors: MS
authors: Bello
authors: Marilda Mendonça
authors: MM
authors: Siqueira
authors: Juan Manuel Piñeiro
authors: JM
authors: Maceira
authors: Maria Genoveva
authors: MG
authors: von Hubinger
authors: José Nelson dos Santos Silva
authors: JN
authors: Couceiro
mesh_terms: D001483:Base Sequence; D001938:Brazil; D005260:Female; D005838:Genotype; D020180:Heteroduplex Analysis / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D010239:Paraguay; D010322:Parvoviridae Infections / Q000821:virology*; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology