title: Glutaredoxin 2a, a mitochondrial isoform, plays a protective role in a human cell line under serum deprivation.
abstract: The roles of mitochondrial glutaredoxin (Grx2a) under serum deprivation were assessed using the human stable HepG2 cell lines overexpressing or down-regulating Grx2a. The Grx2a-overexpressing stable cells displayed enhanced proliferation, decreased reactive oxygen species (ROS) and caspase-3 activity levels, and increased total GSH level, compared to the vector control cells. These characteristics of the overexpressing stable cells were reversed by down-regulating Grx2a in the same cell line. In the limited serum conditions, the Grx2a-overexpressing stable pcDNA3.0/HA-Grx2a cells exhibited higher cellular viabilities and total GSH level, and showed much lower enhancement in ROS and caspase-3 activity levels than the vector control pcDNA3.0/HA cells. However, the Grx2a-down-regulating stable cells gave rise to diminished cellular viabilities and further decreased total GSH level, and contained significantly higher ROS and caspase-3 activity levels, under serum deprivation than the vector control cells. These results suggest that Grx2a plays proliferative and anti-apoptotic roles under serum deprivation.
authors: Department of Biochemistry, College of Natural Sciences, Kangwon National University, 192-1 Hyoja-2-dong, Chuncheon, 200-701, Korea.
authors: Su-Jung
authors: SJ
authors: Kim
authors: Hyun-Joo
authors: HJ
authors: Jung
authors: Hojin
authors: H
authors: Choi
authors: Chang-Jin
authors: CJ
authors: Lim
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D002104:Cadmium / Q000633:toxicity; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D054477:Glutaredoxins / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D020011:Protective Agents / Q000378:metabolism*; D020033:Protein Isoforms / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016895:Culture Media, Serum-Free; C516008:GLRX2 protein, human; D054477:Glutaredoxins; D020011:Protective Agents; D020033:Protein Isoforms; D012333:RNA, Messenger; D002104:Cadmium