title: The osteogenic differentiation of dog bone marrow mesenchymal stem cells in a thermo-sensitive injectable chitosan/collagen/β-glycerophosphate hydrogel: in vitro and in vivo.
abstract: Type I collagen was added to the composite chitosan solution in a ratio of 1:2 to build a physical cross-linked self-forming chitosan/collagen/β-GP hydrogel. Osteogenic properties of this novel injectable hydrogel were evaluated. Gelation time was about 8 min which offered enough time for handling a mixture containing cells and the subsequent injection. Scanning electronic microscopy (SEM) observations indicated good spreading of bone marrow mesenchymal stem cells (BMSCs) in this hydrogel scaffold. Mineral nodules were found in the dog-BMSCs inoculated hydrogel by SEM after 28 days. After subcutaneous injection into nude mouse dorsum for 4 weeks, partial bone formation was observed in the chitosan/collagen/β-GP hydrogel loaded with pre-osteodifferentiated dog-BMSCs, which indicated that chitosan/collagen/β-GP hydrogel composite could induce osteodifferentiation in BMSCs without exposure to a continual supply of external osteogenic factors. In conclusion, the novel chitosan/collagen/β-GP hydrogel composite should prove useful as a bone regeneration scaffold.
authors: Institute of Stomatology, Chinese PLA General Hospital, Haidian District, Beijing, China.
authors: Bin
authors: B
authors: Sun
authors: Wei
authors: W
authors: Ma
authors: Fang
authors: F
authors: Su
authors: Yi
authors: Y
authors: Wang
authors: Jiaqiang
authors: J
authors: Liu
authors: Dongshen
authors: D
authors: Wang
authors: Hongchen
authors: H
authors: Liu
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D004285:Dogs; D005994:Glycerophosphates / Q000737:chemistry*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning
chemical_list: D005994:Glycerophosphates; D020100:Hydrogels; D003094:Collagen; D048271:Chitosan; C031463:beta-glycerophosphoric acid