title: High efficiency transformation by electroporation of Yarrowia lipolytica.
abstract: Yarrowia lipolytica was usually transformed by heat shock, but linearized integrative vectors always resulted in a low transformation efficiency when electroporation was used. To develop a high efficiency integrative transformation method by electroporation of F. lipolytica, we report here that pretreatment of F. lipolytica with 150 mM LiAc for 1 h before electroporation will approximately 30-fold of increase transformation efficiency. A cell concentration of 10(10)/ml and instrument settings of 1.5 kV will generate the highest transformation efficiencies. We have developed a procedure to transform F. lipolytica that will be able to yield an efficiency of 2.1 × 10(4) transformants/ug for integrative linear DNA. With our modifications, the electroporation procedures became a very efficient and reliable tool for F. lipolytica transformation.
authors: Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu, 300, Taiwan.
authors: Jia-Hung
authors: JH
authors: Wang
authors: Wenpin
authors: W
authors: Hung
authors: Shu-Hsien
authors: SH
authors: Tsai
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003470:Culture Media; D004271:DNA, Fungal / Q000235:genetics; D004229:Dithiothreitol / Q000494:pharmacology*; D018274:Electroporation / Q000379:methods*; D014170:Transformation, Genetic / Q000235:genetics*; D025062:Yarrowia / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D000085:Acetates; D003470:Culture Media; D004271:DNA, Fungal; C488804:lithium acetate; D004229:Dithiothreitol