title: [Effect of curcumol on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE-2].
abstract: OBJECTIVE
  To investigate the effect of curcumol on the proliferation, apoptosis and the expression NF-κB in nasopharyngeal carcinoma cell line CNE-2.
  
  
  METHODS
  CNE-2 cells were treated with curcumol at different concentration(12.5, 25, 50, 100 mg/L) and the control group; the effect of proliferation was detected by MTT method; the apoptosis was analyzed by hoechst 33342 flourescence staning and flow cytometry; the expression of NF-κB was detected with western blotting.
  
  
  RESULTS
  After treated with curcumol, CNE-2 cell's proliferation was significantly reduced (P<0.01) and its apoptosis was increased as the curcumol concentration rising, the apoptosis of 100 mg/L curcumol group even can reach to 45.5% and it was a significantly difference compared with control group (P<0.01); the expression of NF-κB was down regulated as raising the curcumol concentration, there was a significantly difference compared with control group (P<0.01).
  
  
  CONCLUSIONS
  Curcumol is capable of significantly inhibiting proliferation and inducing apoptosis of CNE-2' cells in vitro, the mechanism of curcumol anti-tumor may be related to the down regulated of the NF-κB protein level.
authors: School of Pharmaceutical, Guilin Medical University, Guilin, China. juanlovelife@163.com
authors: Juan
authors: J
authors: Wang
authors: Xu
authors: X
authors: Chen
authors: Jian-hong
authors: JH
authors: Zeng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D012717:Sesquiterpenes / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D012717:Sesquiterpenes; C022801:curcumol