title: Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario.
abstract: Antimicrobial susceptibilities and toxin types were determined for 275 Clostridium perfringens isolates collected in Ontario in the spring of 2005. Minimal inhibitory concentrations (MICs) of C. perfringens isolates for 12 antimicrobials used in therapy, prophylaxis, and/or growth promotion of cattle (n = 40), swine (n = 75), turkeys (n = 50), and chickens (n = 100) were determined using the microbroth dilution method. Statistical analyses and MIC distributions showed reduced susceptibility to bacitracin, clindamycin, erythromycin, florfenicol, and tetracycline for some isolates. Reduced susceptibility to bacitracin was identified in chicken (64%) and turkey (60%) isolates. Swine isolates had predominantly reduced susceptibility to clindamycin (28%) and erythromycin (31%), whereas bovine isolates had reduced susceptibility to clindamycin (10%) and florfenicol (10%). Reduced susceptibility to tetracycline was spread across all species. No clear reduced susceptibility, but elevated MIC(50) for virginiamycin was found in chicken isolates in comparison with isolates from other species. Toxin typing revealed that C. perfringens type A is the dominant toxin type isolated in this study across all 4 host species.
authors: Animal Health Laboratory, Laboratory Services, University of Guelph, Ontario. dslavic@uoguelph.ca
authors: Durđa
authors: D
authors: Slavić
authors: Patrick
authors: P
authors: Boerlin
authors: Marta
authors: M
authors: Fabri
authors: Kim C
authors: KC
authors: Klotins
authors: Jennifer K
authors: JK
authors: Zoethout
authors: Pat E
authors: PE
authors: Weir
authors: Debbie
authors: D
authors: Bateman
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003016:Clostridium perfringens / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D024881:Drug Resistance, Bacterial; D005838:Genotype; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009864:Ontario; D013552:Swine / Q000382:microbiology*; D014422:Turkeys / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents