title: Podosome rings generate forces that drive saltatory osteoclast migration.
abstract: Podosomes are dynamic, actin-containing adhesion structures that collectively self-organize as rings. In this study, we first show by observing osteoclasts plated on bead-seeded soft substrates that podosome assemblies, such as rings, are involved in tension forces. During the expansion of a podosome ring, substrate displacement is oriented outward, suggesting that podosomal structures push the substrate away. To further elucidate the function of forces generated by podosomes, we analyze osteoclast migration. Determining the centers of mass of the whole cell (G) and of actin (P), we demonstrate that osteoclasts migrate by "jumps" and that the trajectories of G and P are strongly correlated. The velocity of the center of mass as a function of time reveals that osteoclasts rapidly catch up with podosomal structures in a periodic pattern. We conclude that actin dynamics inside the cell are not only correlated with cell migration, but drive it.
authors: Laboratoire de Physique, UMR 5672, Lyon 69364, France.
authors: Shiqiong
authors: S
authors: Hu
authors: Emmanuelle
authors: E
authors: Planus
authors: Dan
authors: D
authors: Georgess
authors: Christophe
authors: C
authors: Place
authors: Xianghui
authors: X
authors: Wang
authors: Corinne
authors: C
authors: Albiges-Rizo
authors: Pierre
authors: P
authors: Jurdic
authors: Jean-Christophe
authors: JC
authors: Géminard
mesh_terms: D000199:Actins / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D021982:Cell-Matrix Junctions / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D010010:Osteoclasts / Q000378:metabolism / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D059010:Single-Cell Analysis; D013314:Stress, Mechanical; D059008:Time-Lapse Imaging
chemical_list: D000199:Actins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins