title: Radical scavenging capacity and antimutagenic properties of purified proteins from Solanum betaceum fruits and Solanum tuberosum tubers.
abstract: In this study, antioxidant activities in free-radical-mediated oxidative systems and the genotoxic/antigenotoxic effects of two proteins with molecular mass around 17 kDa, purified from Solanum betaceum fruits (cyphomine) and Solanum tuberosum tubers (solamarine), were investigated. Both proteins inhibited uric acid formation with IC(50) values between 55 and 60 μg/mL, and both proteins were able to reduce oxidative damage by scavenging hydroxyl radicals and superoxide anion in a dose-dependent manner. Furthermore, the DPPH• reduction assay showed SC(50) values of 55-73 μg/mL. Cyphomine and solamarine were able to retain their antioxidant activity after heat treatment at 80 °C for 15 min. Allium cepa and Salmonella /microsome assays showed no genotoxic and mutagenic effects. Solamarine showed an antimutagenic effect against a direct mutagen (4-nitro-o-phenylenediamine). Consequently, the present study showed that the investigated proteins are promising ingredients for the development of functional foods with a beneficial impact on human health and an important source for the production of bioactive peptides.
authors: Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina.
authors: Roxana Mabel
authors: RM
authors: Ordóñez
authors: Iris Catiana
authors: IC
authors: Zampini
authors: Fabiola
authors: F
authors: Rodríguez
authors: Florencia
authors: F
authors: Cattaneo
authors: Jorge Esteban
authors: JE
authors: Sayago
authors: María Inés
authors: MI
authors: Isla
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry; D009152:Mutagenicity Tests; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D035281:Plant Tubers / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D032322:Solanum / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010940:Plant Proteins