title: Hydrazide-functionalized magnetic microspheres for the selective enrichment of digested tryptophan-containing peptides in serum.
abstract: The extreme complexity of protein samples is becoming a great challenge for proteomic analysis, especially for those having large dynamic range of protein abundance. To solve this problem, and to overcome the limitation of the current proteomic technologies, a new method using hydrazide-functionalized magnetic microspheres was established in this study. With this method, tryptophan (Trp)-containing peptides can be selectively and sensitively enriched from complex and low-volume samples. Furthermore, combined with 1D-LC-MS/MS analysis, the strategy was successfully applied to the proteomic study of mouse serum. The proportion of Trp-containing peptides was increased from 19.4% to 80.2% through enrichment, and the complexity of the sample was reduced more than two times. An additional 113 Trp-containing peptides and 48 novel proteins were detected compared to the conventional method. This enrichment method provides a means for identifying more proteins as potential biomarkers in serum and other complex samples.
authors: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors: Yanyan
authors: Y
authors: Yu
authors: Mingqi
authors: M
authors: Liu
authors: Guoquan
authors: G
authors: Yan
authors: Yifeng
authors: Y
authors: He
authors: Congjian
authors: C
authors: Xu
authors: Huali
authors: H
authors: Shen
authors: Pengyuan
authors: P
authors: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D008280:Magnetics; D051379:Mice; D008863:Microspheres; D008969:Molecular Sequence Data; D010455:Peptides / Q000097:blood* / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D013379:Substrate Specificity; D014364:Tryptophan
chemical_list: D006834:Hydrazines; D010455:Peptides; C029424:hydrazine; D014364:Tryptophan