title: Selective ex-vivo photothermal ablation of human pancreatic cancer with albumin functionalized multiwalled carbon nanotubes.
abstract: The process of laser-mediated ablation of cancer cells marked with biofunctionalized carbon nanotubes is frequently called "nanophotothermolysis". We herein present a method of selective nanophotothermolisys of pancreatic cancer (PC) using multiwalled carbon nanotubes (MWCNTs) functionalized with human serum albumin (HSA). With the purpose of testing the therapeutic value of these nanobioconjugates, we have developed an ex-vivo experimental platform. Surgically resected specimens from patients with PC were preserved in a cold medium and kept alive via intra-arterial perfusion. Additionally, the HSA-MWCNTs have been intra-arterially administered in the greater pancreatic artery under ultrasound guidance. Confocal and transmission electron microscopy combined with immunohistochemical staining have confirmed the selective accumulation of HSA-MWCNTs inside the human PC tissue. The external laser irradiation of the specimen has significantly produced extensive necrosis of the malign tissue after the intra-arterial administration of HSA-MWCNTs, without any harmful effects on the surrounding healthy parenchyma. We have obtained a selective photothermal ablation of the malign tissue based on the selective internalization of MWCNTs with HSA cargo inside the pancreatic adenocarcinoma after the ex-vivo intra-arterial perfusion.
authors: Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors: Lucian
authors: L
authors: Mocan
authors: Flaviu A
authors: FA
authors: Tabaran
authors: Teodora
authors: T
authors: Mocan
authors: Constantin
authors: C
authors: Bele
authors: Anamaria Ioana
authors: AI
authors: Orza
authors: Ciprian
authors: C
authors: Lucan
authors: Rares
authors: R
authors: Stiufiuc
authors: Ioana
authors: I
authors: Manaila
authors: Ferencz
authors: F
authors: Iulia
authors: Iancu
authors: I
authors: Dana
authors: Florin
authors: F
authors: Zaharie
authors: Gelu
authors: G
authors: Osian
authors: Liviu
authors: L
authors: Vlad
authors: Cornel
authors: C
authors: Iancu
mesh_terms: D055011:Ablation Techniques / Q000379:methods*; D019540:Area Under Curve; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D018869:Heat-Shock Response; D006651:Histocytochemistry; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods; D018613:Microscopy, Confocal; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009336:Necrosis; D010190:Pancreatic Neoplasms / Q000601:surgery*; D012709:Serum Albumin / Q000008:administration & dosage* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D018709:Statistics, Nonparametric; D013696:Temperature
chemical_list: D037742:Nanotubes, Carbon; D012709:Serum Albumin; D016650:Fluorescein-5-isothiocyanate