title: Development and validation of an HPLC-PDA method for the determination of myrsinoic acid B in the extracts of Rapanea ferruginea Mez.
abstract: New, simple, rapid and precise HPLC-PDA method has been developed and validated for quantification of biomarker myrsinoic acid B in stem bark extracts of Rapanea ferruginea Mez. The method employs a Phenomenex C18 column (250 mm × 4.6mm I.D., 5 μm) with acetonitrile:methanol:water (pH 2.6 with phosphoric acid) at 48:30:22 as mobile phase, at a flow rate of 0.7 mL min(-1) and photo diode array (PDA) detection at 270 nm. The validation data show that the method is specific, accurate, precise and robust. The method was linear, over a range of 5-100.0 μg mL(-1), with a limit of detection of 0.369 μg mL(-1) and limit of quantification of 1.233 μg mL(-1). The method has also shown consistent recoveries (average of 101.3% and 0.12% RSD) of the biomarker, with low intra and inter-day relative standard deviation (1.26% and 1.62%, respectively). The evaluated hydroethanolic extract and dry extract presented MAB values of 63.53 and 36.07 mg g(-1), respectively.
authors: Núcleo de Investigações Químico-Farmacêuticas (Niqfar), Programa de Mestrado em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), P.O. BOx 360, 88302-202 Itajaí, SC, Brazil.
authors: Thaisa
authors: T
authors: Baccarin
authors: Rodrigo S
authors: RS
authors: Muceneeki
authors: Tania M B
authors: TM
authors: Bresolin
authors: Rosendo A
authors: RA
authors: Yunes
authors: Angela
authors: A
authors: Malheiros
authors: Ruth M
authors: RM
authors: Lucinda-Silva
mesh_terms: D000475:Alkenes / Q000032:analysis*; D000700:Analgesics / Q000737:chemistry / Q000592:standards; D001572:Benzofurans / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003890:Desiccation; D055808:Drug Discovery; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000592:standards; D029623:Primulaceae / Q000737:chemistry*; D011786:Quality Control; D013997:Time Factors
chemical_list: D000475:Alkenes; D000700:Analgesics; D001572:Benzofurans; D010936:Plant Extracts; C468150:myrsinoic acid B