title: The induction of human CL-P1 expression in hypoxia/reoxygenation culture condition and rat CL-P1 after ischemic/reperfusion treatment.
abstract: BACKGROUND
  Oxidative stress-induced endothelial dysfunction and oxidized low-density lipoprotein (LDL) might play a key role in the pathogenesis of atherosclerosis. We recently identified a vascular endothelial scavenger receptor, collectin placenta 1 (CL-P1), which acts as a receptor for oxidized LDL as well as for microbes.
  
  
  METHODS
  We demonstrate how hypoxic and oxidative stress induced CL-P1 expression and compared their effects with the expression of lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1), an endothelial scavenger receptor expressed by oxidative stress.
  
  
  RESULTS
  Hypoxia/reoxygenation induced CL-P1 mRNA and protein expression in human umbilical vein endothelial cells (HUVECs). The expression of LOX-1 mRNA in these cells peaked slightly at 24 h, while the expression of CL-P1 had an onset at 72 h and was sustained for 120 h after reoxygenation. Furthermore, the exposure of rat carotid artery endothelium to ischemia/reperfusion increased the maximal CL-P1 mRNA expression at 72 h and expression of its protein peaked at 7 days after this treatment. We demonstrate that CL-P1 up-regulation is induced in vitro and in vivo by oxidative stress.
  
  
  CONCLUSIONS
  The inducible expression of CL-P1 by oxidative stress might play a crucial role in endothelial dysfunction or chronic activation leading to the pathogenesis of atherosclerosis.
authors: Department of Microbiology and Immunochemistry, Asahikawa Medical University, Asahikawa 078-8510, Japan.
authors: Satoshi
authors: S
authors: Koyama
authors: Katsuki
authors: K
authors: Ohtani
authors: Jun
authors: J
authors: Fukuzawa
authors: Naoyuki
authors: N
authors: Yao
authors: Mitsuko
authors: M
authors: Fukuda
authors: Seong-Jae
authors: SJ
authors: Jang
authors: Naoyuki
authors: N
authors: Hasebe
authors: Kenjiro
authors: K
authors: Kikuchi
authors: Hiroyuki
authors: H
authors: Itabe
authors: Itsuro
authors: I
authors: Yoshida
authors: Yasuhiko
authors: Y
authors: Suzuki
authors: Nobutaka
authors: N
authors: Wakamiya
mesh_terms: D000249:Adenosine Monophosphate; D000818:Animals; D002460:Cell Line; D037222:Collectins / Q000096:biosynthesis* / Q000235:genetics; D004730:Endothelium, Vascular / Q000378:metabolism; D005260:Female; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D008077:Lipoproteins, LDL; D008297:Male; D011247:Pregnancy; D051381:Rats; D051116:Receptors, Scavenger / Q000096:biosynthesis* / Q000235:genetics; D015427:Reperfusion Injury / Q000503:physiopathology*; D051127:Scavenger Receptors, Class E / Q000096:biosynthesis
chemical_list: C422442:COLEC12 protein, human; C561017:COLEC12 protein, rat; D037222:Collectins; D008077:Lipoproteins, LDL; C495137:OLR1 protein, human; C495136:OLR1 protein, rat; D051116:Receptors, Scavenger; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; D000249:Adenosine Monophosphate