title: Ratiometric detection of mitochondrial thiols with a two-photon fluorescent probe.
abstract: We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90-190 μm depth without interference from other biologically relevant species through the use of two-photon microscopy.
authors: Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
authors: Chang Su
authors: CS
authors: Lim
authors: Goutam
authors: G
authors: Masanta
authors: Hyung Joong
authors: HJ
authors: Kim
authors: Ji Hee
authors: JH
authors: Han
authors: Hwan Myung
authors: HM
authors: Kim
authors: Bong Rae
authors: BR
authors: Cho
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D008928:Mitochondria / Q000737:chemistry*; D017785:Photons; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds