title: Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization.
abstract: Modular bispecific antibodies (BsAb's) that interact directly with a gold surface were engineered for immobilization on biosensing devices. The BsAb's consist of the variable fragments of antigold and antilysozyme antibodies connected via one of three linkers derived from naturally occurring proteins. The BsAb's were bound tightly to both the gold surface and to lysozyme, thus functioning as interface molecules between lysozyme and the gold surface without a substantial loss of antigen-binding activity. The antigen-binding capacity (the ratio of the amount of immobilized lysozyme to the amount of immobilized BsAb) on the gold surface reached 82%. An analysis of the correlation between binding capacity and linker characteristics indicated that the presence of a long, rigid linker sequence derived from a cellulase resulted in a higher antigen-binding capacity than did the presence of a long but relatively flexible glycine-rich linker. This result suggests a strategy for designing linkers suitable for BsAb-based biomolecular immobilization.
authors: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aoba 6-6-11, Aramaki-aza, Aoba-ku, Sendai 980-8579, Japan.
authors: Hideki
authors: H
authors: Watanabe
authors: Kengo
authors: K
authors: Kanazaki
authors: Takeshi
authors: T
authors: Nakanishi
authors: Hidenori
authors: H
authors: Shiotsuka
authors: Satoru
authors: S
authors: Hatakeyama
authors: Masaru
authors: M
authors: Kaieda
authors: Takeshi
authors: T
authors: Imamura
authors: Mitsuo
authors: M
authors: Umetsu
authors: Izumi
authors: I
authors: Kumagai
mesh_terms: D018033:Antibodies, Bispecific / Q000737:chemistry / Q000378:metabolism*; D000941:Antigens / Q000378:metabolism; D032701:Biomimetics / Q000379:methods*; D006046:Gold; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding
chemical_list: D018033:Antibodies, Bispecific; D000941:Antigens; D006046:Gold; D009113:Muramidase