title: The coactivator activator CoAA regulates PEA3 group member transcriptional activity.
abstract: The PEA3 (polyoma enhancer activator 3) group members [ERM (ETS-related molecule), ER81 (ETS-related 81) and PEA3] of the Ets transcription factor family are involved in migration and dissemination processes during organogenesis and cancer development. In the present study, we report that the hnRNP (heterogeneous nuclear ribonucleoprotein)-like protein CoAA (Coactivator activator) interacts with the PEA3 group members and modulates their transcriptional activity. We also demonstrate that the CoAA YQ domain, containing tyrosine/glutamine-rich hexapeptide repeats, is necessary for the interaction, whereas the two N-terminal RRMs (RNA recognition motifs) of CoAA are required to enhance transcriptional activity. Finally, we show that CoAA is involved in the migration-enhancing action of PEA3 on MCF7 human cancer cells, suggesting that CoAA might be an important regulator of PEA3 group member activity during metastasis.
authors: Institut de Biologie de Lille, CNRS/Université Lille Nord de France, Institut Pasteur de Lille, France.
authors: Kathye
authors: K
authors: Verreman
authors: Jean-Luc
authors: JL
authors: Baert
authors: Alexis
authors: A
authors: Verger
authors: Hervé
authors: H
authors: Drobecq
authors: Elisabeth
authors: E
authors: Ferreira
authors: Yvan
authors: Y
authors: de Launoit
authors: Didier
authors: D
authors: Monte
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000502:physiology*; D011817:Rabbits; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology*
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C436317:RBM14 protein, human; D014157:Transcription Factors; C065788:transcription factor PEA3