title: Determination of defensin HNP-1 in human saliva of patients with oral mucosal diseases.
abstract: Saliva is a biological fluid that is easily obtainable and that can give useful information both in systemic and oral diseases. In this study, a chromatographic method was applied to determine the amount of defensin HNP-1 in human saliva of patients with oral mucosal diseases before and after treatments and compared with controls. Defensin human neutrophil peptide-1 (HNP-1) was identified and confirmed. The concentration of HNP-1 in saliva was determined by comparing the area of eluted HNP-1 with that of HNP-1 standard. Linear calibration range of defensin HNP-1 was 0.10 to 0.90 μg/10 μL with R(2) values of 0.996. The concentrations of HNP-1 in the saliva of patients with oral lichen planus, Behçet's disease, and recurrent apthous stomatitis were 33.6 ± 10.6, 15.5 ± 7.6, and 36.3 ± 9.5 μg mL(-1) (mean ± S.D.), respectively. The salivary defensin-1 concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients with oral mucosal diseases, the concentration was significantly higher before treatment than after treatment.
authors: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Selcuk University, Konya, Turkey.
authors: Hasan
authors: H
authors: Küçükkolbaşi
authors: Semahat
authors: S
authors: Küçükkolbaşi
authors: Recep
authors: R
authors: Dursun
authors: Filiz
authors: F
authors: Ayyildiz
authors: Hüseyin
authors: H
authors: Kara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000890:Anti-Infective Agents / Q000032:analysis* / Q000378:metabolism; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000378:metabolism / Q000473:pathology*; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology*; D012463:Saliva / Q000737:chemistry*; D055815:Young Adult; D023084:alpha-Defensins / Q000032:analysis* / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D023084:alpha-Defensins; C406413:human neutrophil peptide 1