title: Chemical characterization of Iraqi propolis samples and assessing their antioxidant potentials.
abstract: Propolis samples, collected from different geographical locations in Iraq (Baghdad, Dahuk, Mosul and Salah ad-Din), were analyzed and assessed for their anti-oxidant activity. Concentrations of phenolic compounds (flavonoids, phenolic acids and their esters) in propolis were estimated using high performance liquid chromatography coupled to electrospray mass spectrometry. Thirty-eight different compounds were identified and 33 of them were polyphenols. Other compounds were tentatively identified as clerodane diterpenoids, and one was considered unknown. Semi-quantitative measurements showed that phenolic acids and their esters were the predominant constituents in propolis extracts, followed by flavones and flavonols, and then flavanones and dihydroflavonols. Propolis samples were further spectrophotometrically characterized using the Folin-Ciocalteu reagent for the determination of total phenolic compounds. The free radical scavenging activities of propolis samples were also evaluated by using the 2,2-diphenyl-1-picrylhydrazyl assay. The results revealed that propolis extracts exhibited strong free radical scavenging activity.
authors: Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq.
authors: Ghassan M
authors: GM
authors: Sulaiman
authors: Khulood W
authors: KW
authors: Al Sammarrae
authors: Ali H
authors: AH
authors: Ad'hiah
authors: Massimo
authors: M
authors: Zucchetti
authors: Roberta
authors: R
authors: Frapolli
authors: Ezia
authors: E
authors: Bello
authors: Eugenio
authors: E
authors: Erba
authors: Maurizio
authors: M
authors: D'Incalci
authors: Renzo
authors: R
authors: Bagnati
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D007493:Iraq; D011429:Propolis / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D011429:Propolis