title: From Cerius² based stereoview to mouse and enzyme: the model systems for discovery of novel urokinase inhibitors.
abstract: Antifibrinolytic therapy during major complex surgery could reduce blood loss and allogeneic transfusion. Novel antagonists of the plasminogen activator and the corresponding model system are of clinical importance. In this paper (1S,2'S,3S)-1-[2-(S)-carboxylindolemethylenemethineaminoeth-1-yl]-2,3,4,5-tetrahydropyrolo[1,2:1,6]pyrazino[3,4:2,3]-1,2,3,4-tetrahydrocarboline-2,5-dione (CIPPC) was presented as a novel antagonist of plasminogen activator, and its blood coagulation and action mechanism were investigated by using a model system which consisted of a mouse-tail bleeding assay, in vitro and in vivo fibrinolysis inhibition assays, a thrombus formation assay and a plasminogen (PLG) electrophoresis assay.
authors: College of Pharmaceutical Sciences, Capital Medical University, Beijing, PR China.
authors: Shenghui
authors: S
authors: Mei
authors: Jiawang
authors: J
authors: Liu
authors: Ming
authors: M
authors: Zhao
authors: Wenjing
authors: W
authors: Wang
authors: Yuji
authors: Y
authors: Wang
authors: Guofeng
authors: G
authors: Wu
authors: Meiqing
authors: M
authors: Zheng
authors: Shiqi
authors: S
authors: Peng
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001798:Blood Proteins / Q000737:chemistry / Q000494:pharmacology*; D005342:Fibrinolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D010958:Plasminogen / Q000378:metabolism*; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001798:Blood Proteins; C031220:urokinase inhibitor; D010958:Plasminogen; D010960:Plasminogen Activators