title: Synthesis, characterization, cytotoxic activity and DNA binding properties of the novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide.
abstract: A novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2-pyridyl)ethylidene]propanedihydrazide was synthesized and characterized by elemental analysis, spectroscopy (NMR and infrared), and X-ray crystal analysis. The complex showed a moderate activity towards Artemia salina. The highest cytotoxic potential of the complex was observed on the epithelial breast cancer (MDA-361) cell line. The investigated complex induced apoptosis, the early apoptotic cells comprising 28.18%, compared to 5.64% of control cells in the same phase. The interaction of the complex with calf thymus DNA (CT-DNA) was monitored by blue shift and hyperchromism in the UV-vis spectra. The observed intrinsic binding constant (K(b)=4.2×10(5)M(-1)) together with structural analysis of the complex indicate the groove binding.
authors: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors: Rabia
authors: R
authors: Eshkourfu
authors: Božidar
authors: B
authors: Čobeljić
authors: Miroslava
authors: M
authors: Vujčić
authors: Iztok
authors: I
authors: Turel
authors: Andrej
authors: A
authors: Pevec
authors: Kristina
authors: K
authors: Sepčić
authors: Manja
authors: M
authors: Zec
authors: Siniša
authors: S
authors: Radulović
authors: Tatjana
authors: T
authors: Srdić-Radić
authors: Dragana
authors: D
authors: Mitić
authors: Katarina
authors: K
authors: Andjelković
authors: Dušan
authors: D
authors: Sladić
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001156:Artemia / Q000187:drug effects / Q000254:growth & development; D001386:Azides / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008314:Malonates / Q000737:chemistry*; D011725:Pyridines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
chemical_list: D000970:Antineoplastic Agents; D001386:Azides; D007364:Intercalating Agents; D008314:Malonates; D011725:Pyridines; D003035:Cobalt; C543930:2-acetylpyridine; D004247:DNA; C427203:calf thymus DNA; C030290:malonic acid