title: The neuromuscular system in freshwater furcocercaria from Belarus. II Diplostomidae, Strigeidae, and Cyathocotylidae.
abstract: The neuromuscular system (NMS) in cercariae of Diplostomum pseudospathaceum, Cotylurus szidati, Australapatemon burti, Holostephanus volgensis, and Paracoenogonimus ovatus was studied with immunocytochemical methods and confocal scanning laser microscopy. The patterns of F-actin in the musculature, 5-HT immunoreactive (-IR), FMRF-amide-IR neuronal elements, and α-tubulin-IR in sensory receptors were investigated. The NMS in the five species studied were compared with each other and with three species of Schistosomatidae studied earlier (Bilharziella polonica, Trichobilharzia szidati, and Trichobilharzia franki). No major structural differences in the musculature, the 5-HT-IR or FMRF-IR neuronal elements were noticed between the cercariae. The minor variations observed in the musculature were related to the size and organization of the muscle fibers. The checked pattern formed by the transverse muscle fibers in the tail stems of D. pseudospathaceum, C. szidati, A. burti, H. volgensis, and P. ovatus was not observed in B. polonica, T. szidati, and T. franki. A trend in the differentiation of the longitudinal muscle fibers in the furca from evenly distributed fibers in H. volgensis and P. ovatus to many bundles in D. pseudospathaceum and two well-organized lateral bundles in C. szidati, A. burti, and Trichobilharzia spp. was observed. The transverse muscle fibers in the furca follow the same trend. The number of 5-HT-IR neurons in the cercarial bodies varied between 10 and 16. In cercariae of H. volgensis and P. ovatus, the central nervous system (CNS) was less centralized compared to the CNS in the other species studied, with only two 5-HT-IR marker neurons in each brain ganglion and the other neurons distributed evenly along the main cords. In the tails of H. volgensis and P. ovatus, many transverse 5-HT-IR comissures were found. In the tails of higher strigeidid cercariae, only a few crosslinks were observed. The number and distribution of sensory receptors on the bodies and tails of the cercarial species differed from each other. A trend in the differentiation of the sensory receptors in the tails was discerned. A process of grouping and decrease in number of ciliated receptors in the stem and in the furca from H. volgensis and P. ovatus to Schistosomatid cercariae took place.
authors: Centre of Parasitology of A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninsky prospect, 33, Moscow 119071, Russia. otolstenkov@gmail.com
authors: Oleg O
authors: OO
authors: Tolstenkov
authors: Ludmila N
authors: LN
authors: Akimova
authors: Nadezhda B
authors: NB
authors: Terenina
authors: Margaretha K S
authors: MK
authors: Gustafsson
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D019835:FMRFamide / Q000032:analysis; D005618:Fresh Water / Q000469:parasitology*; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D032342:Muscle Cells / Q000737:chemistry / Q000166:cytology; D009132:Muscles / Q000033:anatomy & histology / Q000737:chemistry; D009420:Nervous System / Q000033:anatomy & histology / Q000737:chemistry; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D002094:Republic of Belarus; D012701:Serotonin / Q000032:analysis; D014200:Trematoda / Q000033:anatomy & histology* / Q000302:isolation & purification*
chemical_list: D000199:Actins; D012701:Serotonin; D019835:FMRFamide