title: Methods being developed for preparation, delivery and transplantation of a tissue-engineered corneal endothelium.
abstract: Corneal transplantation is the only available treatment to restore vision in patients suffering from endotheliopathies. Tissue engineering offers new alternatives for the replacement of dysfunctional corneal endothelial cells. This review reports the current knowledge on the reconstruction and transplantation of a tissue-engineered corneal endothelium. It describes the cell source, cell isolation, culture, carriers used for reconstruction, as well as alternatives to using carriers. It also reviews the different in vitro and in vivo assessments used to characterize the tissue-engineered endothelium and the different animal models used to test its functionality. Finally, a brief discussion describes the steps and requirements for the technological transfer from bench to bedside.
authors: Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, LOEX-Centre de Recherche FRSQ du Centre Hospitalier Affilié Universitaire de Québec and Département d'Oto-Rhino-laryngologie et d'Ophtalmologie, Faculté de Médecine, Université Laval, Québec, QC, Canada.
authors: Stéphanie
authors: S
authors: Proulx
authors: Isabelle
authors: I
authors: Brunette
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003315:Cornea / Q000098:blood supply*; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery*; D016039:Corneal Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000473:pathology / Q000637:transplantation*; D006801:Humans; D023822:Tissue Engineering / Q000379:methods*; D057170:Translational Research, Biomedical