title: Identification of bacterial target proteins for the salicylidene acylhydrazide class of virulence-blocking compounds.
abstract: A class of anti-virulence compounds, the salicylidene acylhydrazides, has been widely reported to block the function of the type three secretion system of several Gram-negative pathogens by a previously unknown mechanism. In this work we provide the first identification of bacterial proteins that are targeted by this group of compounds. We provide evidence that their mode of action is likely to result from a synergistic effect arising from a perturbation of the function of several conserved proteins. We also examine the contribution of selected target proteins to the pathogenicity of Yersinia pseudotuberculosis and to expression of virulence genes in Escherichia coli O157.
authors: Institute of Infection, Immunity, and Inflammation, College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom.
authors: Dai
authors: D
authors: Wang
authors: Caroline E
authors: CE
authors: Zetterström
authors: Mads
authors: M
authors: Gabrielsen
authors: Katherine S H
authors: KS
authors: Beckham
authors: Jai J
authors: JJ
authors: Tree
authors: Sarah E
authors: SE
authors: Macdonald
authors: Olwyn
authors: O
authors: Byron
authors: Tim J
authors: TJ
authors: Mitchell
authors: David L
authors: DL
authors: Gally
authors: Pawel
authors: P
authors: Herzyk
authors: Arvind
authors: A
authors: Mahajan
authors: Hanna
authors: H
authors: Uvell
authors: Richard
authors: R
authors: Burchmore
authors: Brian O
authors: BO
authors: Smith
authors: Mikael
authors: M
authors: Elofsson
authors: Andrew J
authors: AJ
authors: Roe
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004927:Escherichia coli Infections / Q000188:drug therapy; D019453:Escherichia coli O157 / Q000378:metabolism / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006834:Hydrazines / Q000737:chemistry / Q000494:pharmacology*; D037521:Virulence Factors / Q000096:biosynthesis*; D015011:Yersinia pseudotuberculosis / Q000378:metabolism / Q000472:pathogenicity; D015012:Yersinia pseudotuberculosis Infections / Q000188:drug therapy
chemical_list: D000900:Anti-Bacterial Agents; D006834:Hydrazines; D037521:Virulence Factors