title: Identification and molecular mapping of a single Arabidopsis thaliana locus determining resistance to a phytopathogenic Pseudomonas syringae isolate.
abstract: We present a model pathosystem to dissect genetically the disease resistance response of plants against phytopathogenic bacteria. The interaction between Pseudomonas syringae pathovar maculicola (Psm) and Arabidopsis thaliana displays phenotypic varia-ion which depends on the genotype of both partners. Compatible interactions are defined by sustained in-planta bacterial growth and are normally accompanied of their appearance. For compatible interactions, resistance is defined by limited in-planta bacterial growth accompanied by a typical 'hypersensitive response' (HR). We show that at least parts of this system fit the paradigms of Flor's 'gene-for-gene' hypothesis. We identify functionally a putative bacterial avirulence gene (avrRpm 1) from a Psm isolate which conditions the HR on A. thaliana ecotypes Oy-0 abd Col- 0, but not Nd-0. We also demonstrate that resistance to the Psm strain from which avrRpm1 was isolated segregates as a single trait in the crosses Col-o x Nd-0 and Nd-0 x Oy-0. Furthermore, we map this locus (RPM1) molecularly in the Col-0 x Nd-0 cross to a relatively small interval defined by two RFLP markers on A. thliana chromosome 3. Resistance in the second cross also maps to this locus and co-segregates with resistance to avrRpm1.
authors: Joint first authorsFor correspondance (fax +49 221 5062613).Max-Delbrülck-Laboratorium, Carl-von-LinnU+00E9-Weg 10, D5000 Köln 30, Germany.
authors: Thomas
authors: T
authors: Debener
authors: Joint first authorsFor correspondance (fax +49 221 5062613).Max-Delbrülck-Laboratorium, Carl-von-LinnU+00E9-Weg 10, D5000 Köln 30, Germany.
authors: Hiltrud
authors: H
authors: Lehnackers
authors: Joint first authorsFor correspondance (fax +49 221 5062613).Max-Delbrülck-Laboratorium, Carl-von-LinnU+00E9-Weg 10, D5000 Köln 30, Germany.
authors: Martin
authors: M
authors: Arnold
authors: Joint first authorsFor correspondance (fax +49 221 5062613).Max-Delbrülck-Laboratorium, Carl-von-LinnU+00E9-Weg 10, D5000 Köln 30, Germany.
authors: Jeffery L
authors: JL
authors: Dangl
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D054884:Host-Pathogen Interactions / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D044224:Pseudomonas syringae / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins