title: Decreased motility of human spermatozoa presenting phosphatidylserine membrane translocation-cells selection with the swim-up technique.
abstract: Phosphatidylserine membrane translocation (PST) is considered to be a marker of apoptosis; however, numerous studies have reported on its role in processes not related to cell death. The purpose of the study was to investigate: (1) what is the impact of PST on the motility of spermatozoa, and (2) does the swim-up isolation involve the percentage of cells presenting PST? Semen of 28 normozoospermic men (WHO criteria) was analyzed. High motility spermatozoa were isolated by the swim-up technique. The percentage of spermatozoa with PST in neat semen and after swim-up isolation was assessed with Annexin-V labeled with fluorescein, using flow cytometry technique. The spermatozoas' motility was measured with a computer-assisted analysis system. The kinetic subpopulations of spermatozoa were identified with dedicated software and analyzed regarding PST. Vital spermatozoa with PST demonstrated progressive movement. The motion analysis system revealed a very strong positive correlation between the percentage of vital spermatozoa with PST and the percentage of spermatozoa belonging to the slow subpopulation (r = 0.83; p < 0.05), as well as a very strong negative correlation between the percentage of vital spermatozoa with PST and the percentage of spermatozoa belonging to the rapid subpopulation (r = -0.86; p < 0.05). After the swim-up isolation, the percentage of vital spermatozoa presenting PST significantly decreased (2.4 ± 2.1% vs. 5.2 ± 2.4%; p < 0.05). Spermatozoa with PST present progressive movement; however, their motility is decreased. Isolation of spermatozoa with the swim-up technique eliminates the cells with PST.
authors: Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806, Poznan, Poland. mkotwic@ump.edu.pl
authors: Malgorzata
authors: M
authors: Kotwicka
authors: Magdalena
authors: M
authors: Jendraszak
authors: Izabela
authors: I
authors: Skibinska
authors: Piotr
authors: P
authors: Jedrzejczak
authors: Leszek
authors: L
authors: Pawelczyk
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D010718:Phosphatidylserines / Q000378:metabolism*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
chemical_list: D010718:Phosphatidylserines