title: Inflammatory response in rat lungs with recurrent exposure to welding fumes: a transcriptomic approach.
abstract: As chronic exposure to welding fumes causes pulmonary diseases, such as pneumoconiosis, public concern has increased regarding continued exposure to these hazardous gases in the workplace. In a previous study, the inflammatory response to welding fume exposure was analysed in rat lungs in the case of recurrent exposure and recovery periods. Thus using lung samples, well-annotated by histological observation and biochemical analysis, this study examines the gene expression profiles to identify phenotype-anchored genes corresponding to lung inflammation and the repair phenomenon after recurrent welding fume exposure. Seven genes (Mmp12, Cd5l, LOC50101, LOC69183, Spp1, and Slc26a4) were found to be significantly up-regulated according to the severity of the lung injury. In addition, the transcription and translation of Trem2, which was up-regulated in response to the repair process, were validated using a real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The differentially expressed genes in the exposure and recovery groups were also classified using k-means and hierarchical clustering, plus their toxicological function and canonical pathways were further analysed using Ingenuity Pathways Analysis Software. As a result, this comprehensive and integrative analysis of the transcriptional changes that occur during repeated exposure provides important information on the inflammation and repair processes after welding-fume-induced lung injury.
authors: Division of Research and Development, Korea Institute of Toxicology, Daejeon, Republic of Korea.
authors: Jung-Hwa
authors: JH
authors: Oh
authors: Mi-Jin
authors: MJ
authors: Yang
authors: Jeong-Doo
authors: JD
authors: Heo
authors: Young-Su
authors: YS
authors: Yang
authors: Han-Jin
authors: HJ
authors: Park
authors: Se-Myo
authors: SM
authors: Park
authors: Myung-Sang
authors: MS
authors: Kwon
authors: Chang-Woo
authors: CW
authors: Song
authors: Seokjoo
authors: S
authors: Yoon
authors: Il Je
authors: IJ
authors: Yu
mesh_terms: D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D019570:Inhalation Exposure / Q000032:analysis*; D008168:Lung / Q000737:chemistry / Q000187:drug effects; D055370:Lung Injury / Q000139:chemically induced* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D059467:Transcriptome; D015854:Up-Regulation; D014896:Welding
chemical_list: D000395:Air Pollutants, Occupational