title: [Detection of enteroviruses in urban wastewater in Yopougon, Abidjan].
abstract: OBJECTIVE
  The aim of this study was to determine prevalence of enteroviruses in raw municipal wastewater and specify serotypes circulating.
  
  
  METHODS
  After virus concentration by WHO recommend method, aqueous phase of 68 samples was inoculate on BGM, RD, L20B and Hep2 cells lines. Typing after titration was performed by microplate neutralization test with specific horse immune serum against EV (kit RIVM/WHO). NPEV strains were confirmed by RT-PCR test in 5'NC genomic region. Antigenic and molecular tests of Polioviruses intratypic differentiation were performed to determine vaccine or wild origin of Poliovirus strains.
  
  
  RESULTS
  Viral isolation obtained 43/68 (65.15 %) positives samples. Among these 35/43 (81. 39%) were EV and 8/43 (19.04 %) non EV strain. Among EVs, 34/35 (97.14 %) were NEPV and 2/35 (5.71 %) vaccinal Poliovirus strains type 2 (Sabin-like). NEPV typing gave 77 % untypeable strains by conventionally method. The average virus concentration estimated in the raw sewage is 4.28×10(5) PFU/L.
  
  
  CONCLUSIONS
  Various contacts between people and these waters expose them to health risks.
authors: Unité des virus du système nerveux, département virus épidémique, institut Pasteur de Côte d'Ivoire, BP 490, Abidjan 01, Côte d'Ivoire. akouamc@yahoo.fr
authors: K J
authors: KJ
authors: Momou
authors: C
authors: C
authors: Akoua-Koffi
authors: D S
authors: DS
authors: Akré
authors: E V
authors: EV
authors: Adjogoua
authors: L
authors: L
authors: Tiéoulou
authors: M
authors: M
authors: Dosso
mesh_terms: D002460:Cell Line; D002947:Cities; D007560:Cote d'Ivoire; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D005843:Geography; D006801:Humans; D008828:Microbiological Techniques; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D011891:Rain; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000382:microbiology / Q000821:virology*; D014504:Urban Health; D014773:Virology / Q000379:methods
chemical_list: D004279:DNA, Viral; D012722:Sewage