title: Formulation, pharmacokinetics and biodistribution of Ofloxacin-loaded albumin microparticles and nanoparticles.
abstract: Albumin microparticles containing Ofloxacin (Fluoroquinolone derivative commonly used in hospitals) were formulated by the spray dry method. By decreasing the pump feed rate or the total polymer concentration, a mixture of albumin/hypromellose acetate succinate (HPMCAS) microparticles and nanoparticles (MP/NP), containing Ofloxacin, were formulated. MP/NP were characterized, in vitro (particle size, zeta potential, and encapsulation efficiency). A comparison of the pharmacokinetics and biodistribution of aqueous Ofloxacin and Ofloxacin-loaded MP/NP, in Balb/c mice, revealed that peak concentrations were reduced in the serum, liver, spleen and brain, and a more sustained release was observed in serum and all of the organs tested for Ofloxacin MP/NP, compared to aqueous Ofloxacin. The MP/NP formulation allowed extended release by 24 h in the liver and more than 48 h in the brain. In serum, the elimination rate of Ofloxacin MP/NP is slower, the half life is longer, area under the plasma concentration time curve is decreased and volume of distribution is increased.
authors: Poxvirus and Rabies Branch, Centers for Disease Control, 1600 Clifton Rd, Atlanta, GA 30329, USA. kfl0@cdc.gov
authors: K N
authors: KN
authors: Lee
authors: Y
authors: Y
authors: Ye
authors: J H
authors: JH
authors: Carr
authors: K
authors: K
authors: Karem
authors: M J
authors: MJ
authors: D'Souza
mesh_terms: D000418:Albumins; D000818:Animals; D000900:Anti-Bacterial Agents; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D015242:Ofloxacin / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D010316:Particle Size; D010599:Pharmacokinetics; D014018:Tissue Distribution
chemical_list: D000418:Albumins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D015242:Ofloxacin