title: Effects of autogenous growth factors on heterotopic bone formation of osteogenic cells in small animal model.
abstract: OBJECTIVE
  This study used a new approach to investigate the effective concentrations of growth factors released from platelet concentrate (PC) on the bone formation capacity of osteogenically differentiated rat bone marrow stromal cells (rBMSCs).
  
  
  METHODS
  Rat BMSCs and whole blood were harvested from 40 adult male Spraque-Dawly rats. Rat BMSCs were expanded in an osteogenic medium and seeded on inert collagenous bovine bone matrix (ICBM). Growth factors released from degranulated PC (GFs) containing TGF-β1 1 (25ng/ml)-10ng (250ng/ml) and rhBMP-2 400ng (10μg/ml) were suspended in 40μl platelet poor plasma (PPP) and applied on the ICBM-rBMSC constructs or ICBM only, respectively. The constructs were then transplanted in autologous hosts for 4 weeks. Concurrently, osteoblastic differentiation of rBMSCs on ICBM-rBMSC-PPP constructs was characterized in vitro.
  
  
  RESULTS
  Rat BMSCs in osteogenic medium exhibited phenotypes of mature osteoblasts. The amount of newly formed bone among groups of ICBM-rBMSC-PPP with and without GFs was not significantly different (p>0.05) and was significantly lower than a group of ICBM-PPP-BMP-2 (p<0.05).
  
  
  CONCLUSIONS
  Autogenous GFs had no effect on the capacity of rBMSCs to form new bone. The ability to measure the bone formation capacity of transplanted autologous cells and growth factors in a small animal model was demonstrated.
authors: Department of Oral & Maxillofacial Surgery, Prince of Songkla University, Hat Yai, Songkhla, Thailand. premjit.a@psu.ac.th
authors: Premjit
authors: P
authors: Arpornmaeklong
authors: Prisana
authors: P
authors: Pripatnanont
authors: Winai
authors: W
authors: Kittidumkerng
authors: Winyou
authors: W
authors: Mitarnun
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D001854:Bone Marrow Cells / Q000187:drug effects*; D001857:Bone Matrix; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D015550:Cell Degranulation / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D003094:Collagen; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000601:surgery; D009999:Ossification, Heterotopic / Q000139:chemically induced*; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects*; D010949:Plasma; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D017154:Stromal Cells / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D055396:Bone Morphogenetic Protein 2; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C499469:recombinant human bone morphogenetic protein-2; D015675:Osteocalcin; D003094:Collagen; D000469:Alkaline Phosphatase