title: Platelet proteome in healthy volunteers who smoke.
abstract: Smoking accelerates atherosclerosis and is a well-known risk factor for acute cardiovascular complications; however, the mechanisms of these effects have not been completely clarified. Recently developed proteomic approaches may offer new clues when combined with well-established functional tests. Platelet proteome of healthy smokers and non-smokers was resolved by two-dimensional difference gel electrophoresis, compared by Decyder software and identified by mass spectrometry analysis (nano-LC-MS/MS). In smokers, three proteins (Factor XIII-A subunit, platelet glycoprotein IIb and beta-actin) were significantly up-regulated, whereas WDR1 protein and chaperonine HSP60 were down-regulated. Furthermore, the highest scored network derived by Ingenuity Pathway Analysis using the modulated proteins as input showed the involvement of several proteins to be related to inflammation and apoptosis. Platelet function tests and the levels of markers of platelet and leukocyte activation were not different in smokers vs. non-smoker subjects. The platelet proteomic approach confirms that cigarette smoking triggers several inflammatory reactions and may help clarify some of the molecular mechanisms of smoke effect on cellular systems relevant for vascular integrity and human health.
authors: Laboratory of Analytical Techniques and Proteomics, Research Laboratories, Catholic University, Campobasso, Italy. dellacorte2007@yahoo.it
authors: Anna
authors: A
authors: Della Corte
authors: Chiara
authors: C
authors: Tamburrelli
authors: Marilena
authors: M
authors: Crescente
authors: Lucia
authors: L
authors: Giordano
authors: Marco
authors: M
authors: D'Imperio
authors: Michela
authors: M
authors: Di Michele
authors: Maria Benedetta
authors: MB
authors: Donati
authors: Giovanni
authors: G
authors: De Gaetano
authors: Domenico
authors: D
authors: Rotilio
authors: Chiara
authors: C
authors: Cerletti
mesh_terms: D000328:Adult; D002450:Cell Communication / Q000502:physiology; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012907:Smoking / Q000097:blood*
chemical_list: D020543:Proteome