title: Laboratory methods in the study of endometrial Claudin-4.
abstract: Immunohistochemistry is a suitable method for the detection of proteins from the Claudin family and several antibodies are commercially available for the detection of Claudin congeners. Immunodetection of Caludin-4 in the paraffin-embedded specimens might be a useful tool for studying the role of these proteins in the cyclic transformation of the endometrium and its role in the endometrial receptivity; furthermore, other components of the junctional zone involved in the transformational process of the endometrium can be detected by means of immunohistochemistry/immunofluorescence with several polyclonal or monoclonal antibodies. The aim of this chapter is to comprehensively overview the materials and methods to perform the endometrial biopsy and to detect Claudin-4 in paraffin-embedded samples of endometrium. Additionally, the interpretation of the results is addressed.
authors: Huntington Center for Reproductive Medicine of Brazil, Sao Paulo, Brazil.
authors: Paulo
authors: P
authors: Serafini
authors: André Monteiro
authors: AM
authors: da Rocha
authors: Gary Daniel
authors: GD
authors: Smith
authors: Eduardo Leme Alves
authors: EL
authors: da Motta
authors: Edmund Chada
authors: EC
authors: Baracat
mesh_terms: D001710:Biotin; D062506:Claudin-4; D004717:Endometrium / Q000378:metabolism*; D004801:Eosine Yellowish-(YS); D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism*; D016612:Paraffin Embedding / Q000379:methods
chemical_list: C568157:CLDN4 protein, human; D062506:Claudin-4; D008565:Membrane Proteins; D001710:Biotin; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin