title: Thrombolytic along with anti-platelet activity of crinumin, a protein constituent of Crinum asiaticum.
abstract: Several anticoagulants, anti-platelet and thrombolytic medications are used for the treatment of thrombotic disorders. Anti-coagulants and anti-platelet agents prevent the formation of blood clots but do not dissolve existing clots, whereas thrombolytic agents are able to dissolve a clot but emboli can form even after successful treatment. Thus, none of them provide a permanent and complete solution. In this regard a single molecule that could both dissolve the clot and prevent the formation of new clots would be useful in the treatment of thrombotic diseases. Crinumin, a stable and active (in many adverse conditions) serine protease, shows plasmin-like fibrinolytic activity and inhibits platelet aggregation and P-selectin exposure, as established by photography, phase contrast microscopy, whole blood optical Lumi-aggregometry and flow cytometry. Crinumin could be an efficient and inexpensive therapeutic agent for the treatment and prevention of thromboembolic diseases.
authors: Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors: Kunwar Awaneesh
authors: KA
authors: Singh
authors: Manasa K
authors: MK
authors: Nayak
authors: Medicherla V
authors: MV
authors: Jagannadham
authors: Debabrata
authors: D
authors: Dash
mesh_terms: D000925:Anticoagulants / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D031407:Crinum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005342:Fibrinolysis / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D005434:Flow Cytometry; D006801:Humans; D019007:P-Selectin / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D013923:Thromboembolism / Q000097:blood / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control; D013927:Thrombosis / Q000097:blood / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control
chemical_list: D000925:Anticoagulants; D005343:Fibrinolytic Agents; D019007:P-Selectin; D010936:Plant Extracts; D010940:Plant Proteins; C569001:crinumin, Crinum asiaticum