title: Comparison of the host specificities of two bacteroidales quantitative PCR assays used for tracking human fecal contamination.
abstract: The sewage-associated real-time quantitative PCR (qPCR) assays BacHum and HF183 SYBR were compared for specificity against local fecal sources. Both assays were equally sensitive to sewage, but BacHum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.
authors: Donald Bren School of Environmental Science and Management, University of California, Santa Barbara, CA 93106-5131, USA. lvandewerfhorst@bren.ucsb.edu
authors: Laurie C
authors: LC
authors: Van De Werfhorst
authors: Bram
authors: B
authors: Sercu
authors: Patricia A
authors: PA
authors: Holden
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D041963:Bacteroidetes / Q000235:genetics* / Q000302:isolation & purification*; D002415:Cats; D046091:Charadriiformes; D004285:Dogs; D005189:False Positive Reactions; D005243:Feces / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011821:Raccoons; D012680:Sensitivity and Specificity