title: Binding of zearalenone, aflatoxin B1, and ochratoxin A by yeast-based products: a method for quantification of adsorption performance.
abstract: A methodology was developed to quantify the efficiency of yeast-based products for adsorption of three mycotoxins: zearalenone (ZEA), aflatoxin B(1) (AFB(1)), and ochratoxin A (OTA). Eight products were tested (yeast cell wall or inactivated yeast). The described experimental protocol based on in vitro tests provided reliable isotherms for each mycotoxin. The most suitable models were the Hill model for ZEA, the Langmuir model for AFB(1), and the Freundlich model for OTA. From these models, original mathematical affinity criteria were defined to quantify the product adsorption performances for each mycotoxin. The best yeast product, a yeast cell wall from baker's yeast, can adsorb up to 68% of ZEA, 29% of AFB(1), and 62% of OTA, depending on the mycotoxin concentrations. The adsorption capacity largely depended both on yeast composition and mycotoxin, but no direct correlation between yeast composition and adsorption capacity was found, confirming that adsorption of mycotoxin on yeast-based products involves complex phenomena. The results of this study are useful for comparing the adsorption efficiency of various yeast products and understanding the mechanisms involved in adsorption.
authors: Université de Toulouse, Institut National Poyltechnique de Toulouse, École Nationale Supérieure des Ingénieurs en Arts Chimiques et Technologiques, Laboratoire de Génie Chimique, UMR-CNRS 5503, 4 allée Emile Monso, BP 44362, F-31030 Toulouse cedex 4, France. claire.joanniscassan@ensiacet.fr
authors: Claire
authors: C
authors: Joannis-Cassan
authors: Mariana
authors: M
authors: Tozlovanu
authors: Kheira
authors: K
authors: Hadjeba-Medjdoub
authors: Nathalie
authors: N
authors: Ballet
authors: Annie
authors: A
authors: Pfohl-Leszkowicz
mesh_terms: D000327:Adsorption; D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019540:Area Under Curve; D003257:Consumer Product Safety; D005506:Food Contamination / Q000517:prevention & control*; D006801:Humans; D015651:Mycotoxicosis / Q000517:prevention & control; D009793:Ochratoxins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015003:Yeasts / Q000737:chemistry / Q000502:physiology*; D015025:Zearalenone / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A; D015025:Zearalenone; D016604:Aflatoxin B1