title: An artificial enzyme-based assay: DNA detection using a peroxidase-like copper-creatinine complex.
abstract: We report an artificial enzyme-based DNA assay using a peroxidase-like copper (Cu)-creatinine complex as a catalyst for 3,3',5,5'-tetramethylbenzidine (TMB) oxidation. The assay employs double signal amplification and a homogeneous catalytic reaction: (i) fast catalytic growth of Cu on a gold (Au) nanoparticle (NP) label forms a thick Cu layer (first amplification); (ii) dissolution of the Cu layer generates many Cu-creatinine complexes per NP (generation of homogeneous catalysts); (iii) peroxidase-like Cu-creatinine complexes rapidly convert TMB into a colored product (second amplification). To investigate the effect of ligand on the catalytic activities of Cu complexes, the kinetics of catalytic TMB oxidation is tested with and without using imidazole ring-containing ligands (creatinine, imidazole, and poly(l-histidine)). Both fast oxidation of TMB and slow further oxidation of the colored product are required for high signal-to-background ratios. Cu-creatinine complex allows relatively fast oxidation and slow further oxidation. Fast seed-mediated Cu growth on Au NP and slow Cu autonucleation (i.e., slow formation of Cu NP in the absence of Au NP) are also required for high signal-to-background ratios. In tris-EDTA (tris(hydroxymethyl)aminomethane-ethylenediaminetetraacetic acid) buffer (pH 7.7) containing high concentrations of Cu(2+) (90 mM), ascorbic acid (50mM), and Mg(2+) (200 mM), Cu growth on Au NP is very fast and autonucleation is significantly suppressed. Fast catalytic oxidation by Cu-creatinine complex along with fast Cu growth on Au NP allows a detection limit of 0.1 pM for DNA in a simple microplate format.
authors: Department of Chemistry and Chemistry Institute of Functional Materials, Pusan National University, Busan 609-735, Republic of Korea.
authors: Amardeep
authors: A
authors: Singh
authors: Srikanta
authors: S
authors: Patra
authors: Jeong-Ah
authors: JA
authors: Lee
authors: Kang Hyun
authors: KH
authors: Park
authors: Haesik
authors: H
authors: Yang
mesh_terms: D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D003404:Creatinine / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010084:Oxidation-Reduction; D009195:Peroxidase / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D006046:Gold; D003300:Copper; D004247:DNA; D003404:Creatinine; D009195:Peroxidase