title: Transmission of Fusarium boothii mycovirus via protoplast fusion causes hypovirulence in other phytopathogenic fungi.
abstract: There is increasing concern regarding the use of fungicides to control plant diseases, whereby interest has increased in the biological control of phytopathogenic fungi by the application of hypovirulent mycoviruses as a possible alternative to fungicides. Transmission of hypovirulence-associated double-stranded RNA (dsRNA) viruses between mycelia, however, is prevented by the vegetative incompatibility barrier that often exists between different species or strains of filamentous fungi. We determined whether protoplast fusion could be used to transmit FgV1-DK21 virus, which is associated with hypovirulence on F. boothii (formerly F. graminearum strain DK21), to F. graminearum, F. asiaticum, F. oxysporum f. sp. lycopersici, and Cryphonectria parasitica. Relative to virus-free strains, the FgV1-DK21 recipient strains had reduced growth rates, altered pigmentation, and reduced virulence. These results indicate that protoplast fusion can be used to introduce FgV1-DK21 dsRNA into other Fusarium species and into C. parasitica and that FgV1-DK21 can be used as a hypovirulence factor and thus as a biological control agent.
authors: Department of Agricultural Biotechnology and Center for Fungal Pathogenesis, Seoul National University, Seoul, Korea.
authors: Kyung-Mi
authors: KM
authors: Lee
authors: Jisuk
authors: J
authors: Yu
authors: Moonil
authors: M
authors: Son
authors: Yin-Won
authors: YW
authors: Lee
authors: Kook-Hyung
authors: KH
authors: Kim
mesh_terms: D061046:Biological Control Agents; D005670:Fusarium / Q000166:cytology* / Q000502:physiology*; D010935:Plant Diseases / Q000382:microbiology*; D011523:Protoplasts / Q000166:cytology*; D014908:Triticum / Q000382:microbiology
chemical_list: D061046:Biological Control Agents