title: Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients.
abstract: Gastric cancer is the fourth most common cancer worldwide, with a high rate of death and low 5-year survival rate. To date, there is a lack of efficient therapeutic protocols for gastric cancer. Recent studies suggest that cancer stem cells (CSCs) are responsible for tumor initiation, invasion, metastasis, and resistance to anticancer therapies. Thus, therapies that target gastric CSCs are attractive. However, CSCs in human gastric adenocarcinoma (GAC) have not been described. Here, we identify CSCs in tumor tissues and peripheral blood from GAC patients. CSCs of human GAC (GCSCs) that are isolated from tumor tissues and peripheral blood of patients carried CD44 and CD54 surface markers, generated tumors that highly resemble the original human tumors when injected into immunodeficient mice, differentiated into gastric epithelial cells in vitro, and self-renewed in vivo and in vitro. Our findings suggest that effective therapeutic protocols must target GCSCs. The capture of GCSCs from the circulation of GAC patients also shows great potential for identification of a critical cell population potentially responsible for tumor metastasis, and provides an effective protocol for early diagnosis and longitudinal monitoring of gastric cancer.
authors: Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
authors: Tie
authors: T
authors: Chen
authors: Kun
authors: K
authors: Yang
authors: Jianhua
authors: J
authors: Yu
authors: Wentong
authors: W
authors: Meng
authors: Dandan
authors: D
authors: Yuan
authors: Feng
authors: F
authors: Bi
authors: Fang
authors: F
authors: Liu
authors: Jie
authors: J
authors: Liu
authors: Bing
authors: B
authors: Dai
authors: Xinzu
authors: X
authors: Chen
authors: Fang
authors: F
authors: Wang
authors: Fan
authors: F
authors: Zeng
authors: Hong
authors: H
authors: Xu
authors: Jiankun
authors: J
authors: Hu
authors: Xianming
authors: X
authors: Mo
mesh_terms: D000230:Adenocarcinoma / Q000097:blood* / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D013274:Stomach Neoplasms / Q000097:blood* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1