title: Research on the relationship between thick greasy tongue fur formation and vascular endothelial cell permeability with the protein expression of zonula occludens-1.
abstract: OBJECTIVE
  To determine the relationship of thick greasy tongue fur formation and permeability of vascular endothelial cells (ECs) with the protein expression of zonula occludens-1 (ZO-1).
  
  
  METHODS
  Sprague Dawley rats were randomly divided into a model group of severe acute pancreatitis (SAP) and a sham-operated (SO) group. The SAP rats were further divided into two subgroups on the basis of tongue-coating status: a thick greasy tongue fur group (SAP-TGF) and a normal tongue fur group (SAP-NF). Six rats were chosen randomly from every group mentioned above for an Evans blue assay 5 days after model establishment. For the histomorphology analysis, the expressions of ZO-1 protein and mRNA were studied by hematoxylin-eosin (H&E) staining, transmission electron microscope, Western blot, and Q-PCR using blood and tongue tissues, which were collected from 8 rats randomly chosen from each group.
  
  
  RESULTS
  The papillae density of the rat tongue surface and the caryocinesis frequency of the basal layer were significantly increased in the SAP-TGF group compared with the SO group (P<0.05). Evans blue levels in the tongue tissue of the SAP-TGF group were significantly higher than that of the SO and SAP-NF groups (P<0.05). Vascular ECs were wider and obviously fissured in the SAP-TGF group under transmission electron microscope observation. The protein and mRNA expression of ZO-1 in the SAP-TGF group were lower than those in the SAP-NF (P<0.05).
  
  
  CONCLUSIONS
  Reproductive activity enhancement of glossal epithelial cells was one of the main characteristics of thick greasy tongue fur formation. An increase in vasopermeability was closely associated with thick greasy tongue fur formation. Tight junction structural variation of vascular ECs might play an important role in the pathological and physiological process of thick greasy tongue fur formation.
authors: Department of Infection and Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors: Wen-Jie
authors: WJ
authors: Qi
authors: Miao-Miao
authors: MM
authors: Zhang
authors: Hong
authors: H
authors: Wang
authors: Yan
authors: Y
authors: Wen
authors: Bao-En
authors: BE
authors: Wang
authors: Shu-Wen
authors: SW
authors: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002463:Cell Membrane Permeability; D042783:Endothelial Cells / Q000166:cytology*; D005070:Evans Blue / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014059:Tongue / Q000473:pathology* / Q000648:ultrastructure; D062826:Zonula Occludens-1 Protein
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C569044:Tjp1 protein, rat; D062826:Zonula Occludens-1 Protein; D005070:Evans Blue