title: Protective effects of the preconditioning with different doses of sodium aescinate on tourniquet-induced ischemic reperfusion.
abstract: OBJECTIVE
  To evaluate the protective effects of sodium aescinate (SA) preconditioning on the tourniquet-induced ischemia-reperfusion (I/R) injury after limbs operation.
  
  
  METHODS
  Seventy-five patients with grade I-II issued by American Society of Anesthesiology undergoing lower limb operation were randomly assigned to 3 groups: the control group, low-dose SA-treated group and high-dose SA-treated group; each group enrolled 25 patients. The patients were treated with 5 mg and 10 mg SA 30 min before tourniquet inflation in the two treatment groups separately, while the patients in the control group received normal saline. Venous blood samples were obtained before tourniquet was inflated (T0 baseline). And 5 (T1), 10 (T2), 20 (T3) min after tourniquet was released. The nitric oxide (NO), malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined by commercial kits. Meanwhile, arterial pressure (MAP) and heart rate (HR) were monitored from an automatic invigilator.
  
  
  RESULTS
  In the control group, MDA and NO levels were increased, and SOD and MAP were decreased significantly after tourniquet deflation compared to T0 baseline (P<0.05). After tourniquet deflation, MDA and NO levels in the two treated groups were significantly decreased; meanwhile, SOD levels and MAP were increased, and the variations of HR were more stable compared with the control group (all P<0.05). There was no significant difference in all of the above between the two treated groups (P>0.05).
  
  
  CONCLUSIONS
  The protective effects of SA preconditioning on tourniquet-induced limb I/R injury might possibly contribute to the increasing of SOD levels, and MAP and the decreasing of MDA and NO levels.
authors: Department of Anesthesiology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors: Guang
authors: G
authors: Yang
authors: Su-Yang
authors: SY
authors: Cui
authors: Li-Bing
authors: LB
authors: Huang
mesh_terms: D000328:Adult; D004305:Dose-Response Relationship, Drug; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D019194:Ischemic Preconditioning; D007866:Leg / Q000098:blood supply* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020011:Protective Agents / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D012964:Sodium / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D014111:Tourniquets; D055986:Vital Signs / Q000187:drug effects; D055815:Young Adult
chemical_list: D020011:Protective Agents; D012964:Sodium