title: PNPLA3 polymorphism influences liver fibrosis in unselected patients with type 2 diabetes.
abstract: BACKGROUND
  Recently, it has been shown that an allele in the adiponutrin (PNPLA3) gene was strongly associated with increased liver fat content (LFC) and liver fibrosis independent of visceral adiposity and insulin resistance.
  
  
  OBJECTIVE
  In this study, we set out to determine whether the PNPLA3 rs738409 polymorphism was associated with liver fibrosis in unselected patients with type 2 diabetes.
  
  
  METHODS
  Two hundred and thirty-four patients with type 2 diabetes were included in this study.
  
  
  METHODS
  LFC was evaluated using (1) H-MR spectroscopy; fibrosis was measured using the non-invasive FibroTest(®).
  
  
  RESULTS
  Advanced liver fibrosis (stage F2 or above) was observed in 10.2% of the patients while 149 (63.6%) patients had steatosis. The prevalence of steatosis and fibrosis was higher in minor G allele carriers than that in C allele homozygote carriers (70.3 vs 57.1%; P=0.04 and 14.7 vs 7.5%; P=0.07 respectively). In multivariate analysis, the predictive variables for advanced liver fibrosis were age (≥60) (P=0.005), sex (female) (P=0.004) and rs 738409 PNPLA3 polymorphism (P=0.01); body mass index (BMI) and LFC were not associated with liver fibrosis.
  
  
  CONCLUSIONS
  This study confirms that in patients with type 2 diabetes who were not selected for liver abnormalities, liver fibrosis was related to the rs738409 polymorphism independent of BMI or LFC.
authors: Centre de Recherche, INSERM Unité 866, Université de Bourgogne, CHU du Bocage, Dijon, France. jean-michel.petit@chu-dijon.fr
authors: Jean M
authors: JM
authors: Petit
authors: Boris
authors: B
authors: Guiu
authors: David
authors: D
authors: Masson
authors: Laurence
authors: L
authors: Duvillard
authors: Valerie
authors: V
authors: Jooste
authors: Perrine
authors: P
authors: Buffier
authors: Benjamin
authors: B
authors: Bouillet
authors: Marie-Claude
authors: MC
authors: Brindisi
authors: Isabelle
authors: I
authors: Robin
authors: Philippe
authors: P
authors: Gambert
authors: Bruno
authors: B
authors: Verges
authors: Jean-Pierre
authors: JP
authors: Cercueil
authors: Patrick
authors: P
authors: Hillon
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D016009:Chi-Square Distribution; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D005234:Fatty Liver / Q000201:enzymology / Q000235:genetics; D005260:Female; D005602:France; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006720:Homozygote; D006801:Humans; D008049:Lipase / Q000235:genetics*; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D008103:Liver Cirrhosis / Q000097:blood / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D016015:Logistic Models; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease; D016017:Odds Ratio; D010641:Phenotype; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D012720:Severity of Illness Index; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent
chemical_list: D015415:Biomarkers; D008049:Lipase; D008565:Membrane Proteins; C533682:PNPLA3 protein, human; D000217:Acyltransferases; D054512:Phospholipases A2, Calcium-Independent