title: [Determination of histamine in fish and fish products by tandem solid-phase extraction].
abstract: A simple and practical method was developed for the determination of histamine in fish and fish products by solid-phase extraction and fluorescence derivatization. Histamine was extracted with trichloroacetic acid. The extract was neutralized and diluted with phosphate buffer (pH 6.8), and cleaned up with a tandem-connected octadecyl silica (ODS) and strong cation exchange silica (SCX) cartridge. After removal of the solvent, histamine was derivatized with fluorescamine and analyzed by ion-paired reversed-phase high-performance liquid chromatography with fluorescence detection. Recovery tests of histamine from six kinds of fish and fish products showed acceptable recovery (83-92%) with low relative standard deviation (less than 5%). This method could be useful for determination of histamine in fish.
authors: Osaka Prefectural Institute of Public Health. awazu@iph.pref.osaka.jp
authors: Kaoru
authors: K
authors: Awazu
authors: Chie
authors: C
authors: Nomura
authors: Mizuka
authors: M
authors: Yamaguchi
authors: Hirotaka
authors: H
authors: Obana
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D006632:Histamine / Q000032:analysis*; D052616:Solid Phase Extraction
chemical_list: D006632:Histamine