title: The TallyHo polygenic mouse model of diabetes: implications in wound healing.
abstract: BACKGROUND
  Impairments in wound healing represent a significant source of morbidity and mortality in patients with diabetes. To help uncover the derangements associated with diabetic wound healing, murine animal models have been extensively used. In this article, the authors present results, and the accompanying wound healing implications, from experiments across three validated wound healing models using a newer polygenic strain of diabetes.
  
  
  METHODS
  The authors investigated the wound healing impairments of the TallyHo/JnJ diabetic mouse strain, using three validated wound healing models: an incisional model, a splinted excisional model, and a cutaneous ischemia-reperfusion injury model. Appropriate control strain mice were used for comparison. Wounds were analyzed using gross, histologic, and molecular techniques.
  
  
  RESULTS
  TallyHo mice displayed deficits across all three wound healing models. There was a reduced resistance/response to oxidative stress and a global decrease in the initial inflammatory response to healing. In addition, there was a global decrease in the stimulus for angiogenesis and collagen formation, ultimately leading to reduced reepithelialization, granulation tissue formation, wound contraction, and wound tensile strength. Gross and histologic findings were corroborated with molecular data, which revealed a significant down-regulation of important cytokines, including vascular endothelial growth factor, neutrophilic attractant protein-2, monocyte chemoattractant protien-1, heme oxygenase-1, interleukin-1β, and interleukin-6, when normalized to the control strain (p<0.05).
  
  
  CONCLUSIONS
  The TallyHo polygenic mouse model of diabetes demonstrates predictable and clinically relevant wound healing impairments that offer important implications into the derangements of diabetic wound healing observed clinically. Therapeutics targeting these specific derangements could provide improvements in the care of diabetic wounds.
authors: Chicago, Ill. From the Laboratory for Wound Repair and Regeneration, Division of Plastic and Reconstructive Surgery, Northwestern University.
authors: Donald W
authors: DW
authors: Buck
authors: Da P
authors: DP
authors: Jin
authors: Matthew
authors: M
authors: Geringer
authors: Seok Jong
authors: SJ
authors: Hong
authors: Robert D
authors: RD
authors: Galiano
authors: Thomas A
authors: TA
authors: Mustoe
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D062109:Dermatologic Surgical Procedures; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology* / Q000601:surgery; D004195:Disease Models, Animal; D015536:Down-Regulation; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008967:Molecular Biology; D012313:RNA / Q000032:analysis; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D012016:Reference Values; D015427:Reperfusion Injury / Q000473:pathology / Q000601:surgery; D012867:Skin / Q000293:injuries / Q000473:pathology; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
chemical_list: D016207:Cytokines; D012313:RNA