title: An ultrasensitive immunosensor array for determination of staphylococcal enterotoxin B.
abstract: Staphylococcal enterotoxin B (SEB) is a potent gastrointestinal toxin and is heat resistant. SEB is also a potential bioterrorism agent. The ability to measure accurately very low amounts of staphylococcal enterotoxin B in food and other samples is very important. A highly sensitive and stable sandwich fluorescence immunoassay based on a pair of monoclonal antibodies against SEB which were produced by us was developed. Classical sandwich immunoassay was adopted and the glass slides were used as the base of the immunologic reaction. The functionalized fluorescent core-shell silica nanoparticles were used as labels. The fluorescence issued from the labels was detected by a laser-induced fluorescence millimeter sensor array detection platform. The fluorescence intensity has a linear relationship with the amount of SEB in the range of 50 pg/mL-5 ng/mL, and the detection limit of SEB was 20 pg/mL (the absolute detection limit was 0.02 pg). The relative standard deviation (RSD) for 5 parallel measurements of SEB (1 ng/mL) was 9.2%.
authors: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
authors: Xiaoming
authors: X
authors: Zhang
authors: Fei
authors: F
authors: Liu
authors: Ruifang
authors: R
authors: Yan
authors: Pan
authors: P
authors: Xue
authors: Yongming
authors: Y
authors: Li
authors: Lili
authors: L
authors: Chen
authors: Chaojun
authors: C
authors: Song
authors: Cui
authors: C
authors: Liu
authors: Boquan
authors: B
authors: Jin
authors: Zhujun
authors: Z
authors: Zhang
authors: Kun
authors: K
authors: Yang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D005456:Fluorescent Dyes / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D007834:Lasers; D053758:Nanoparticles / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D004768:Enterotoxins; D005456:Fluorescent Dyes; C031818:enterotoxin B, staphylococcal