title: Correlation of immunohistologic and perfusion vascular parameters with MR contrast enhancement using image-guided biopsy specimens in gliomas.
abstract: OBJECTIVE
  The purpose of this study was to correlate the status of magnetic resonance contrast enhancement with immunohistologic vascular parameters such as microvascular cellular proliferation (MVCP), microvascular density (MVD), vascular endothelial growth factor receptor-2 (VEGFR-2) expression, and World Health Organization (WHO) grade obtained from image-guided biopsy specimens. We also compared perfusion computed tomography (PCT) parameters such as cerebral blood volume (CBV), cerebral blood flow (CBF), and permeability surface area-product (PS) with the presence or absence of contrast enhancement.
  
  
  METHODS
  A total of 26 image-guided biopsy specimens in 16 patients with treatment naive gliomas were obtained from contrast-enhancing (CE) and nonenhancing (NE) regions of the glioma. Contrast enhancement status was correlated with MVD, MVCP, VEGFR-2 expression, and WHO grade obtained from the biopsy specimen as well as with the PCT parameters.
  
  
  RESULTS
  Contrast enhancement showed statistically significant correlation with MVCP (P = .003) and PS (P = .007) when compared with various immunohistologic and perfusion vascular parameters. WHO grade of the biopsy specimen showed statistically significant correlation with contrast enhancement (P = .002), MVCP (P < .001), and PS values (P = .028).
  
  
  CONCLUSIONS
  Contrast enhancement in gliomas is primarily from a break in blood-brain barrier as evidenced by its correlation with PS and MVCP, whereas it was not statistically correlated with CBV and MVD even though it showed a positive trend. Contrast enhancement also showed significant correlation with WHO grade suggesting a biopsy from CE region in a heterogeneous glioma probably will still yield the most aggressive part of the glioma is also shown by its association with MVCP and PS estimates.
authors: Division of Neuroradiology, Department of Radiology, Henry Ford Health System, Detroit, MI 48202, USA. rajanj@rad.hfh.edu
authors: Rajan
authors: R
authors: Jain
authors: Jayant
authors: J
authors: Narang
authors: Jorge
authors: J
authors: Gutierrez
authors: Lonni R
authors: LR
authors: Schultz
authors: Lisa
authors: L
authors: Scarpace
authors: Mark
authors: M
authors: Rosenblum
authors: Tom
authors: T
authors: Mikkelsen
authors: Jack P
authors: JP
authors: Rock
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy / Q000379:methods*; D001783:Blood Flow Velocity; D001810:Blood Volume; D001932:Brain Neoplasms / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D002560:Cerebrovascular Circulation; D016009:Chi-Square Distribution; D003287:Contrast Media; D005260:Female; D057918:Fiducial Markers; D005910:Glioma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D008833:Microcirculation; D008875:Middle Aged; D009367:Neoplasm Staging; D011446:Prospective Studies; D014057:Tomography, X-Ray Computed; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D003287:Contrast Media; D040301:Vascular Endothelial Growth Factor Receptor-2