title: Inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus in chicken bursal lymphocytes.
abstract: The aim of this study was to investigate the inhibitory effect of Sargassum polysaccharide on oxidative stress induced by infectious bursa disease virus (IBDV) in chicken bursal lymphocytes. The levels of IL-1β, IL-8, IL-10, TNF-α, MCP-1, reduced glutathione and reactive oxygen species in chicken bursal lymphocytes treated with IBDV or both IBDV and Sargassum polysaccharide were measured, and the activities of superoxide dimutase and glutathione peroxidase were evaluated. Our results showed that oxidative stress appeared when chicken bursal lymphocytes were incubated with IBDV for 8h at 100 TCID(50). Sargassum polysaccharide inhibited oxidative stress by increasing the amount of reduced glutathione, promoting the activities of superoxide dimutase and glutathione peroxidase and reducing the level of reactive oxygen species. The polysaccharide also raised IL-1β, IL-8, IL-10 and TNF-α levels in cells infected with IBDV. These findings suggest that Sargassum polysaccharide acts against infection by elevating antioxidant capacity and cytokine levels in chicken bursal lymphocytes.
authors: College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, PR China.
authors: Ling
authors: L
authors: Zhang
authors: Ting-Jun
authors: TJ
authors: Hu
authors: Hong-Li
authors: HL
authors: Liu
authors: Xue-Hong
authors: XH
authors: Shuai
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002060:Bursa of Fabricius / Q000187:drug effects / Q000473:pathology / Q000821:virology*; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000821:virology*; D002849:Chromatography, Gas; D016207:Cytokines / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007243:Infectious bursal disease virus / Q000187:drug effects / Q000502:physiology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000473:pathology* / Q000821:virology; D009005:Monosaccharides / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D016207:Cytokines; D016166:Free Radical Scavengers; D009005:Monosaccharides; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione