title: Avian influenza viruses infect primary human bronchial epithelial cells unconstrained by sialic acid α2,3 residues.
abstract: Avian influenza viruses (AIV) are an important emerging threat to public health. It is thought that sialic acid (sia) receptors are barriers in cross-species transmission where the binding preferences of AIV and human influenza viruses are sias α2,3 versus α2,6, respectively. In this study, we show that a normal fully differentiated, primary human bronchial epithelial cell model is readily infected by low pathogenic H5N1, H5N2 and H5N3 AIV, which primarily bind to sia α2,3 moieties, and replicate in these cells independent of specific sias on the cell surface. NHBE cells treated with neuraminidase prior to infection are infected by AIV despite removal of sia α2,3 moieties. Following AIV infection, higher levels of IP-10 and RANTES are secreted compared to human influenza virus infection, indicating differential chemokine expression patterns, a feature that may contribute to differences in disease pathogenesis between avian and human influenza virus infections in humans.
authors: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.
authors: Christine M
authors: CM
authors: Oshansky
authors: Jennifer A
authors: JA
authors: Pickens
authors: Konrad C
authors: KC
authors: Bradley
authors: Les P
authors: LP
authors: Jones
authors: Geraldine M
authors: GM
authors: Saavedra-Ebner
authors: James P
authors: JP
authors: Barber
authors: Jackelyn M
authors: JM
authors: Crabtree
authors: David A
authors: DA
authors: Steinhauer
authors: S Mark
authors: SM
authors: Tompkins
authors: Ralph A
authors: RA
authors: Tripp
mesh_terms: D000293:Adolescent; D000818:Animals; D001717:Birds / Q000821:virology*; D001980:Bronchi / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D018925:Chemokines / Q000378:metabolism; D002923:Cilia / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology / Q000821:virology*; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology*; D007251:Influenza, Human / Q000473:pathology / Q000821:virology*; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology; D017201:Virus Shedding / Q000187:drug effects / Q000502:physiology
chemical_list: D018925:Chemokines; D011956:Receptors, Cell Surface; C092488:sialic acid receptor; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid