title: Inhibition of D-amino acid oxidase activity by antipsychotic drugs evaluated by a fluorometric assay using D-kynurenine as substrate.
abstract: A facile fluorometric assay using D-kynurenine as a substrate was utilized for evaluating the inhibition of D-amino acid oxidase (DAAO), which is one of the products of a susceptibility gene for schizophrenia, by commercial antipsychotic drugs, namely, chlorpromazine (CPZ), carbamazepine, sulpiride, quetiapine, and imipramine. CPZ inhibited DAAO (65.8 ± 13.2 μM, n = 3) as reported previously, and other drugs also inhibited DAAO activity. Among these, quetiapine had the smallest IC(50) value (19.5 ± 2.60 μM, n = 3). The proposed assay can be useful for the evaluation or screening of DAAO-inhibitory drugs.
authors: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, Chiba, Japan.
authors: Sumiko
authors: S
authors: Iwasa
authors: Hiroshi
authors: H
authors: Tabara
authors: Ziyu
authors: Z
authors: Song
authors: Moe
authors: M
authors: Nakabayashi
authors: Yuusaku
authors: Y
authors: Yokoyama
authors: Takeshi
authors: T
authors: Fukushima
mesh_terms: D014150:Antipsychotic Agents / Q000494:pharmacology*; D002746:Chlorpromazine / Q000494:pharmacology; D003605:D-Amino-Acid Oxidase / Q000037:antagonists & inhibitors*; D003987:Dibenzothiazepines / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005470:Fluorometry / Q000379:methods*; D057166:High-Throughput Screening Assays; D007737:Kynurenine; D000069348:Quetiapine Fumarate; D012559:Schizophrenia / Q000235:genetics
chemical_list: D014150:Antipsychotic Agents; D003987:Dibenzothiazepines; D000069348:Quetiapine Fumarate; D007737:Kynurenine; D003605:D-Amino-Acid Oxidase; D002746:Chlorpromazine