title: Streptococcus mutans biofilm adhesion on composite resin surfaces after different finishing and polishing techniques.
abstract: This study evaluated Streptococcus mutans biofilm adhesion on the surface of three composite resins (nanofilled, Filtek Z350, 3M ESPE, Salt Lake City, UT, USA; nanohybrid, Vit-1-escence, Ultradent Products, South Jordan, UT, USA; and microhybrid, Esthet X, Dentsply, Milford, DE, USA) following different finishing and polishing techniques. Sixty standardized samples (6 × 3 mm) of each composite were produced and randomly divided into three finishing and polishing treatments (n=20): 1) control group: composite resin surface in contact with Mylar matrix strips with no finishing or polishing performed, 2) Sof-Lex aluminum oxide disc technique (3M ESPE, and 3) carbide bur finishing and Astrobrush polishing technique (Ultradent). Half the samples of each group were incubated in human saliva for 1 hour, and all the samples were subjected to S mutans (ATCC 35688) biofilm development. The mean log of CFU/mL present in the S mutans biofilm was calculated, and data were statistically analyzed by three-way analysis of variance and the Tukey test (p<0.05). Human saliva incubation promoted a significant increase of bacterial adherence on all three of the composites' surfaces, regardless of the polishing treatment performed (p<0.05). Of the three, the nanofilled composite (Filtek Z350) had the lowest bacterial adherence with each of the finishing and polishing techniques despite the presence or absence of human saliva (p<0.05). Mylar matrix strips (control group) promoted the lowest bacterial adhesion on the surface of the microhybrid and nanofilled composites in the absence of human saliva.
authors: Department of Biosciences and Oral Diagnosis, Univ. Estadual Paulista, Sao Jose dos Campos, Brazil.
authors: C A
authors: CA
authors: Pereira
authors: E
authors: E
authors: Eskelson
authors: V
authors: V
authors: Cavalli
authors: P C S
authors: PC
authors: Liporoni
authors: A O C
authors: AO
authors: Jorge
authors: M A
authors: MA
authors: do Rego
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D001422:Bacterial Adhesion / Q000502:physiology*; D058491:Bacterial Load; D018441:Biofilms / Q000254:growth & development; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry*; D003775:Dental Polishing / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry; D011093:Polyethylene Terephthalates / Q000737:chemistry; D012463:Saliva / Q000382:microbiology; D017655:Silicon Compounds / Q000737:chemistry; D013295:Streptococcus mutans / Q000502:physiology*; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D017973:Tungsten Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D017554:Carbon Compounds, Inorganic; D003188:Composite Resins; D003764:Dental Materials; C466112:Esthet-X; D011093:Polyethylene Terephthalates; D017655:Silicon Compounds; D017973:Tungsten Compounds; C515948:Vit-l-escence Trans Ice; C533204:filtek Z350; D014867:Water; C002802:tungsten carbide; C025539:Lavsan; D000537:Aluminum Oxide; C022088:silicon carbide