title: High-performance thin-layer chromatography/mass spectrometry for the analysis of neutral glycosphingolipids.
abstract: This mini-review summarizes the protocol we have developed for the analysis of neutral glycosphingolipids (GSLs) by high-performance thin layer chromatography (HPTLC)-mass spectrometry (MS). We also present results obtained using this glycolipidomic approach to study neutral GSLs from mouse kidney, spleen, and small intestine. Finally, we discuss what is required for further development of this method, as well as what is expected for the future of glycolipid biology.
authors: Tokai University, Kanagawa, Japan. akmszk@tokai-u.jp
authors: Akemi
authors: A
authors: Suzuki
authors: Masao
authors: M
authors: Miyazaki
authors: Junko
authors: J
authors: Matsuda
authors: Azusa
authors: A
authors: Yoneshige
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D020383:Neutral Glycosphingolipids / Q000032:analysis* / Q000737:chemistry; D009928:Organ Specificity; D012996:Solutions
chemical_list: D020383:Neutral Glycosphingolipids; D012996:Solutions