title: Evaluation of FKBP and DHFR based destabilizing domains in Saccharomyces cerevisiae.
abstract: Two orthogonal destabilizing domains have been developed based on mutants of human FKBP12 as well as bacterial DHFR and these engineered domains have been used to control protein concentration in a variety of contexts in vitro and in vivo. FKBP12 based destabilizing domains cannot be rescued in the yeast Saccharomyces cerevisiae; ecDHFR based destabilizing domains are not degraded as efficiently in S. cerevisiae as in mammalian cells or Plasmodium, but provide a starting point for the development of domains with increased signal-to-noise in S. cerevisiae.
authors: Department of Chemical and Systems Biology, Stanford University, CA, United States.
authors: Rishi
authors: R
authors: Rakhit
authors: Sarah R
authors: SR
authors: Edwards
authors: Mari
authors: M
authors: Iwamoto
authors: Thomas J
authors: TJ
authors: Wandless
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D013762:Tetrahydrofolate Dehydrogenase; D022061:Tacrolimus Binding Protein 1A