title: Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.).
abstract: Qfhi.nau-5A is a major quantitative trait locus (QTL) against Fusarium graminearum infection in the resistant wheat germplasm Wangshuibai. Genetic analysis using BC(3)F(2) and BC(4)F(2) populations, derived from selfing two near-isogenic lines (NIL) heterozygous at Qfhi.nau-5A that were developed, respectively, with Mianyang 99-323 and PH691 as the recurrent parent, showed that Qfhi.nau-5A inherited like a single dominant gene. This QTL was thus designated as Fhb5. To fine map it, these two backcross populations and a recombinant inbred line (RIL) population derived from Nanda2419 × Wangshuibai were screened for recombinants occurring between its two flanking markers Xbarc56 and Xbarc100. Nineteen NIL recombinants were identified from the two backcross populations and nine from the RIL population. In the RIL recombinant selection process, selection against Fhb4 present in the RIL population was incorporated. Genotyping these recombinant lines with ten markers mapping to the Xbarc56-Xbarc100 interval revealed four types of Mianyang 99-323-derived NIL recombinants, three types of PH691-derived NIL recombinants, and four types of RIL recombinants. In different field trials, the percentage of infected spikes of these lines displayed a distinct two-peak distribution. The more resistant class had over 55% less infection than the susceptible class. Common to these resistant genotypes, the 0.3-cM interval flanked by Xgwm304 and Xgwm415 or one of these two loci was derived from Wangshuibai, while none of the susceptible recombinants had Wangshuibai chromatin in this interval. This interval harboring Fhb5 was mapped to the pericentromeric C-5AS3-0.75 bin through deletion bin mapping. The precise localization of Fhb5 will facilitate its utilization in marker-assisted wheat breeding programs.
authors: Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors: Shulin
authors: S
authors: Xue
authors: Feng
authors: F
authors: Xu
authors: Mingzhi
authors: M
authors: Tang
authors: Yan
authors: Y
authors: Zhou
authors: Guoqiang
authors: G
authors: Li
authors: Xia
authors: X
authors: An
authors: Feng
authors: F
authors: Lin
authors: Haibin
authors: H
authors: Xu
authors: Haiyan
authors: H
authors: Jia
authors: Lixia
authors: L
authors: Zhang
authors: Zhongxin
authors: Z
authors: Kong
authors: Zhengqiang
authors: Z
authors: Ma
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics; D005670:Fusarium / Q000276:immunology; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology*; D040641:Quantitative Trait Loci; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D005819:Genetic Markers