title: Phosphoproteins regulated by heat stress in rice leaves.
abstract: BACKGROUND
  High temperature is a critical abiotic stress that reduces crop yield and quality. Rice (Oryza sativa L.) plants remodel their proteomes in response to high temperature stress. Moreover, phosphorylation is the most common form of protein post-translational modification (PTM). However, the differential expression of phosphoproteins induced by heat in rice remains unexplored.
  
  
  METHODS
  Phosphoprotein in the leaves of rice under heat stress were displayed using two-dimensional electrophoresis (2-DE) and Pro-Q Diamond dye. Differentially expressed phosphoproteins were identified by MALDI-TOF-TOF-MS/MS and confirmed by Western blotting.
  
  
  RESULTS
  Ten heat-phosphoproteins were identified from twelve protein spots, including ribulose bisphos-phate carboxylase large chain, 2-Cys peroxiredoxin BAS1, putative mRNA binding protein, Os01g0791600 protein, OSJNBa0076N16.12 protein, putative H(+)-transporting ATP synthase, ATP synthase subunit beta and three putative uncharacterized proteins. The identification of ATP synthase subunit beta was further validated by Western-blotting. Four phosphorylation site predictors were also used to predict the phosphorylation sites and the specific kinases for these 10 phosphoproteins.
  
  
  CONCLUSIONS
  Heat stress induced the dephosphorylation of RuBisCo and the phosphorylation of ATP-β, which decreased the activities of RuBisCo and ATP synthase. The observed dephosphorylation of the mRNA binding protein and 2-Cys peroxiredoxin may be involved in the transduction of heat-stress signaling, but the functional importance of other phosphoproteins, such as H+-ATPase, remains unknown.
authors: Key Laboratory of Ministry of Education for Genetic, Breeding and Multiple Utilization of Crops, Fuzhou 350002, China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Xinhai
authors: X
authors: Chen
authors: Key Laboratory of Ministry of Education for Genetic, Breeding and Multiple Utilization of Crops, Fuzhou 350002, China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Wenfeng
authors: W
authors: Zhang
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Baoqian
authors: B
authors: Zhang
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Jiechao
authors: J
authors: Zhou
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Yongfei
authors: Y
authors: Wang
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Qiaobin
authors: Q
authors: Yang
authors: College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Yuqin
authors: Y
authors: Ke
authors: Key Laboratory of Ministry of Education for Genetic, Breeding and Multiple Utilization of Crops, Fuzhou 350002, China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.|College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors: Huaqin
authors: H
authors: He