title: Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes.
abstract: BACKGROUND
  The objective of this study was to compare the transfection efficiency of anionic liposomes coated with polyethylenimine (PEI) with that of PEI and Lipofectamine 2000(™) using the plasmid DNA encoding green fluorescent protein in a human hepatoma (Huh7) cell line.
  
  
  METHODS
  Factors affecting transfection efficiency, including type of surfactant, ratio of phosphatidylcholine (PC)/surfactant, carrier/DNA weight ratio, and the presence of serum have been investigated. Anionic liposomes, composed of PC and anionic surfactants, ie, sodium oleate (NaO), sodium taurocholate (NaT), or zwitterionic surfactant (3-[{3-cholamidopropyl}-dimethylammonio]-1-propanesulfonate, CHAPS) at molar ratios of 10:1, 10:1.5, and 10:2 were prepared by the sonication method. Subsequently, they were coated with PEI to produce polycationic liposomes (PCL).
  
  
  RESULTS
  PCL was able to condense with pDNA depending on the PCL/DNA weight ratio. PCL composed of PC:NaO (10:2) showed higher transfection efficiency than NaT and CHAPS at all weight ratios tested. Higher transfection efficiency and gene expression were observed when the carrier/DNA weight ratio increased. The highest transfection efficiency was found at a weight ratio of 0.5.
  
  
  CONCLUSIONS
  This PCL showed remarkably high transfection efficiency with low cytotoxicity to Huh7 cells in vitro, in comparison with PEI and Lipofectamine 2000.
authors: Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand. praneet@su.ac.th
authors: Praneet
authors: P
authors: Opanasopit
authors: Orapan
authors: O
authors: Paecharoenchai
authors: Theerasak
authors: T
authors: Rojanarata
authors: Tanasait
authors: T
authors: Ngawhirunpat
authors: Uracha
authors: U
authors: Ruktanonchai
mesh_terms: D000838:Anions; D002412:Cations / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D002793:Cholic Acids; D024202:Electrophoretic Mobility Shift Assay; D018014:Gene Transfer Techniques; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010713:Phosphatidylcholines / Q000737:chemistry; D011094:Polyethyleneimine / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014162:Transfection / Q000379:methods*
chemical_list: D000838:Anions; D002412:Cations; D002793:Cholic Acids; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D011094:Polyethyleneimine; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate