title: Dependence of the cytotoxicity of multi-walled carbon nanotubes on the culture medium.
abstract: This study examined the influence of multi-walled carbon nanotubes (MWNTs) on the growth of the unicellular protozoan Tetrahymena pyriformis. Contrary to the findings from most other investigations, our experiment indicated that MWNTs stimulated growth of the cells cultured in proteose peptone yeast extract medium (PPY). Atomic force microscopy images and thermogravimetric analysis showed the spontaneous formation of peptone-MWNT conjugates in the medium by noncovalent binding. Uptake of large amounts of the conjugates by Tetrahymena pyriformis was responsible for growth stimulation, evidenced by images with fluorescently labelled peptone. After the PPY medium was replaced by a filtrated pond water medium (FPW), however, inhibition of the growth of cells exposed to MWNTs occurred. Measurements of the level of malondialdehyde and superoxide dismutase activity demonstrated further that MWNTs might be either toxic or nontoxic, depending on the medium used to cultivate Tetrahymena pyriformis. The biological effects of the interaction of MWNTs with some composites in culture media would be helpful for understanding the mechanisms of the toxicity of carbon nanotubes to living systems.
authors: Laboratory of Nano-biology and Medicine, Shanghai Institute of Applied Physics, Chinese Academy of Science, Shanghai 201800, People's Republic of China. Graduate School of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors: Ying
authors: Y
authors: Zhu
authors: Tiecheng
authors: T
authors: Ran
authors: Yuguo
authors: Y
authors: Li
authors: Jinxue
authors: J
authors: Guo
authors: Wenxin
authors: W
authors: Li