title: Investigating the embryo/larval toxic and genotoxic effects of γ irradiation on zebrafish eggs.
abstract: Eggs/larval of freshwater fish (Danio rerio) were exposed to low dose rates of external gamma radiation (from 1 to 1000 mGy d(-1)) over a 20-day period, with the objective of testing the appropriateness of the 10 mGy d(-1) guideline suggested by the IAEA. The present study examines different endpoints, mortality and hatching time and success of embryos as well as the genotoxicity of γ-irradiations (after 48 h). The 20-day embryo-larval bioassay showed an enhanced larval resistance to starvation after chronic exposure to γ irradiation (from low 1 mGy d(-1) to high dose rate 1000 mGy d(-1)) and an acceleration in hatching time. Gamma irradiation led to increased genotoxic damage Ito zebrafish egg (40-50% DNA in tail in Comet assay) from the lowest dose rate (1 mGy d(-1)). Possible mechanisms of γ radiotoxicity and implications for radioprotection are discussed.
authors: Laboratoire de Radioécologie et d'Ecotoxicologie, Institut de Radioprotection et de Sûreté Nucléaire, Cadarache, Bat 186, BP3, 13115 Saint-Paul-lez-Durance Cedex, France. olivier.simon@irsn.fr
authors: O
authors: O
authors: Simon
authors: S
authors: S
authors: Massarin
authors: F
authors: F
authors: Coppin
authors: T G
authors: TG
authors: Hinton
authors: R
authors: R
authors: Gilbin
mesh_terms: D000818:Animals; D054750:Ecotoxicology / Q000379:methods*; D047108:Embryonic Development / Q000528:radiation effects; D005720:Gamma Rays; D007814:Larva / Q000528:radiation effects; D009152:Mutagenicity Tests / Q000379:methods*; D010063:Ovum / Q000528:radiation effects; D018570:Risk Assessment / Q000379:methods; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*