title: Enhanced protection against infection with transmissible gastroenteritis virus in piglets by oral co-administration of live attenuated Salmonella enterica serovar Typhimurium expressing swine interferon-α and interleukin-18.
abstract: The enhanced effect of cytokine combinations has been assessed empirically, based on their immunobiological mechanisms. However, far less is known of the enhanced protection of practical cytokine combinations against viral infection in the livestock industry, due to cost and production issues associated with mass administration. This study demonstrates the enhanced protection of oral co-administration of swine interferon-α (swIFN-α) and interleukin-18 (swIL-18) against infection with transmissible gastroenteritis virus (TGEV) in piglets using attenuated Salmonella enterica serovar Typhimurium as carrier of cytokine proteins. A single oral co-administration of S. enterica serovar Typhimurium expressing swIFN-α and swIL-18 induced enhanced alleviation of the severity of diarrhea caused by TGEV infection, compared to piglets administered S. enterica serovar Typhimurium expressing swIFN-α or swIL-18 alone. This enhancement was further observed by the reduction of TGEV shedding and replication, and the expression of IFN-stimulated gene products in the intestinal tract. The results suggest that the combined administration of the swIFN-α and swIL-18 cytokines using attenuated S. enterica serovar Typhimurium as an oral carrier provides enhanced protection against intestinal tract infection with TGEV.
authors: College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju, Republic of Korea.
authors: Byung Min
authors: BM
authors: Lee
authors: Young Woo
authors: YW
authors: Han
authors: Seong Bum
authors: SB
authors: Kim
authors: Md Masudur
authors: MM
authors: Rahman
authors: Erdenebileg
authors: E
authors: Uyangaa
authors: Jin Hyoung
authors: JH
authors: Kim
authors: Yoon Seok
authors: YS
authors: Roh
authors: Bumseok
authors: B
authors: Kim
authors: Sang-Bae
authors: SB
authors: Han
authors: Jin Tae
authors: JT
authors: Hong
authors: Koanhoi
authors: K
authors: Kim
authors: Seong Kug
authors: SK
authors: Eo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005260:Female; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control* / Q000821:virology; D007110:Immunity, Active; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology*; D020382:Interleukin-18 / Q000235:genetics / Q000276:immunology*; D007422:Intestines / Q000276:immunology* / Q000821:virology; D058751:Livestock; D051379:Mice; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012486:Salmonella typhimurium / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D013552:Swine; D014162:Transfection; D005760:Transmissible gastroenteritis virus / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D019562:Viral Load / Q000187:drug effects / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects / Q000276:immunology
chemical_list: D016898:Interferon-alpha; D020382:Interleukin-18; D011993:Recombinant Fusion Proteins; D014613:Vaccines, Attenuated