title: Antibody responses to the immunodominant Cryptosporidium gp15 antigen and gp15 polymorphisms in a case-control study of cryptosporidiosis in children in Bangladesh.
abstract: Although Cryptospridium hominis is the dominant Cryptosporidium species infecting humans, immune responses to cognate antigens in C. hominis-infected persons have not been reported. We investigated antibody responses to the immunodominant gp15 antigen from C. hominis and C. parvum, in C. hominis-infected Bangladeshi children less than five years of age with diarrhea (cases) and uninfected children with diarrhea (controls). We also investigated polymorphisms in the C. hominis gp15 sequence from cases. Serum IgG responses to gp15 from both species were significantly greater in cases than controls. In spite of polymorphisms in the gp15 sequence, there was a significant correlation between antibody levels to gp15 from both species, indicating cross-reactivity to conserved epitopes. Cases with acute diarrhea had a significantly greater serum IgA response to gp15 compared with those with persistent diarrhea, suggesting that this response may be associated with protection from prolonged disease. These findings support further investigation of gp15 as a vaccine candidate.
authors: Division of Geographic Medicine and Infectious Diseases, Tufts Medical Center and Department of Public Health and Community Medicine, Tufts University School of Medicine, Boston, Massachusetts 02111, USA. gallison@tuftsmedicalcenter.org
authors: Genève M
authors: GM
authors: Allison
authors: Kathleen A
authors: KA
authors: Rogers
authors: Anoli
authors: A
authors: Borad
authors: Sabeena
authors: S
authors: Ahmed
authors: Mohammad Mahbubul
authors: MM
authors: Karim
authors: Anne V
authors: AV
authors: Kane
authors: Patricia L
authors: PL
authors: Hibberd
authors: Elena N
authors: EN
authors: Naumova
authors: Stephen B
authors: SB
authors: Calderwood
authors: Edward T
authors: ET
authors: Ryan
authors: Wasif A
authors: WA
authors: Khan
authors: Honorine D
authors: HD
authors: Ward
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001459:Bangladesh; D016022:Case-Control Studies; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000276:immunology*; D003458:Cryptosporidium / Q000276:immunology*; D003967:Diarrhea / Q000276:immunology* / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007223:Infant; D007231:Infant, Newborn; D011110:Polymorphism, Genetic
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G