title: Improved in vitro development of cloned bovine embryos using S-adenosylhomocysteine, a non-toxic epigenetic modifying reagent.
abstract: In this study, fibroblast cells were stably transfected with mouse POU5F1 promoter-driven enhanced green fluorescent protein (EGFP) to investigate the effect of S-adenosylhomocysteine (SAH), the reversible non-toxic inhibitor of DNA-methyltransferases (DNMTs), at different intervals post-fusion on in vitro development of cloned bovine embryos. Treatment with SAH for 12 hr resulted in 54.6 ± 7.7% blastocyst production, which was significantly greater than in vitro fertilized embryos (IVF: 37.2 ± 2.7%), cloned embryos treated with SAH for 72 hr (31.0 ± 7.6%), and control cloned embryos (34.6 ± 3.6%). The fluorescence intensities of the EGFP-POU5F1 reporter gene at all intervals of SAH treatment, except of 72 hr, were significantly higher than control somatic cell nuclear transfers (SCNT) embryos. The intensity of DNA-methylation in cloned embryos treated with SAH for 48 hr was similar to that of IVF embryos, and was significantly lower than the other SCNT groups. The levels of H3K9 acetylation in all SCNT groups were significantly lower than IVF embryos. Real-time PCR analysis of gene expression revealed significantly higher expression of POU5F1 in cloned versus IVF blastocysts. Neither embryo production method (SCNT vs. IVF) nor the SAH treatment interval affected expression of the BCL2 gene. Cloned embryos at all intervals of SAH treatment, except for 24 hr, had significantly increased VEGF transcript compared to IVF and control SCNT embryos. It was suggested that the time interval of DNMT inhibition may have important consequences on different in vitro features of bovine SCNT, and the improving effects of DNMT inhibition on developmental competency of cloned embryos are restricted to a specific period of time preceding de novo methylation.
authors: Faculty of Agriculture, Department of Animal Science, University Putra of Malayasia, Kualalumpur, Malaysia.
authors: Shahram
authors: S
authors: Jafari
authors: Morteza S
authors: MS
authors: Hosseini
authors: Mahdi
authors: M
authors: Hajian
authors: Mohsen
authors: M
authors: Forouzanfar
authors: Farnoosh
authors: F
authors: Jafarpour
authors: Parvaneh
authors: P
authors: Abedi
authors: Somayyeh
authors: S
authors: Ostadhosseini
authors: Hassan
authors: H
authors: Abbasi
authors: Hamid
authors: H
authors: Gourabi
authors: Abdol H
authors: AH
authors: Shahverdi
authors: Ahmad Vosough Taghi
authors: AV
authors: Dizaj
authors: Maryam
authors: M
authors: Anjomshoaa
authors: Wahid
authors: W
authors: Haron
authors: Noor
authors: N
authors: Noorshariza
authors: Halimhatoon
authors: H
authors: Yakub
authors: Mohammad H
authors: MH
authors: Nasr-Esfahani
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000378:metabolism; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D019175:DNA Methylation / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000502:physiology; D044127:Epigenesis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D012435:S-Adenosylhomocysteine / Q000494:pharmacology*; D014162:Transfection
chemical_list: D006657:Histones; D050814:Octamer Transcription Factor-3; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012435:S-Adenosylhomocysteine