title: Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells.
abstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 μg mL(-1) and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.
authors: Nanotechnology Center, Applied Science Department, University of Arkansas, Little Rock, AR, USA.
authors: Alokita
authors: A
authors: Karmakar
authors: Stacie M
authors: SM
authors: Bratton
authors: Enkeleda
authors: E
authors: Dervishi
authors: Anindya
authors: A
authors: Ghosh
authors: Meena
authors: M
authors: Mahmood
authors: Yang
authors: Y
authors: Xu
authors: Lamya Mohammed
authors: LM
authors: Saeed
authors: Thikra
authors: T
authors: Mustafa
authors: Dan
authors: D
authors: Casciano
authors: Anna
authors: A
authors: Radominska-Pandya
authors: Alexandru S
authors: AS
authors: Biris
mesh_terms: D000704:Analysis of Variance; D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D005029:Ethylenediamines / Q000737:chemistry*; D005260:Female; D016158:Genes, p53; D015316:Genetic Therapy / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013818:Thermogravimetry
chemical_list: D005029:Ethylenediamines; D037742:Nanotubes, Carbon; D049452:Green Fluorescent Proteins; C031234:ethylenediamine; D053148:Caspase 3