title: Boyden chamber.
abstract: The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
authors: Barts and The London School of Medicine and Dentistry, Blizard Institute of Cell and Molecular Science, London, UK. m.falasca@qmul.ac.uk
authors: Marco
authors: M
authors: Falasca
authors: Claudio
authors: C
authors: Raimondi
authors: Tania
authors: T
authors: Maffucci
mesh_terms: D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D054443:Cell Migration Assays / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D002633:Chemotaxis; D003470:Culture Media; D006801:Humans; D008567:Membranes, Artificial; D016062:Porosity
chemical_list: D003470:Culture Media; D008567:Membranes, Artificial