title: Skin-healing activity and toxicological evaluation of a proteinase fraction from Carica candamarcensis.
abstract: Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of skin healing activity by the group of proteinases known as P1G10. By using a hairless mouse model, we compared the healing effect following topical application of various concentrations of P1G10. The data confirm that healing actions take place between 0.1 and 1%, without adverse local irritation or systemic toxicological action after a prolonged period of use. The wound healing effect is unaltered when P1G10 is previously inhibited with iodoacetamide. The low permeation of the hydrosoluble formulation Polawax(®) supports the maintenance of the drug at the site of application. These results extend the healing properties of these groups of enzymes in situations of dermatological trauma and open the way to future clinical applications.
authors: Department of Pharmacology, Instituto de Ciencias Biologicas, Antonio Carlos, Brazil.
authors: Fernanda O
authors: FO
authors: Lemos
authors: Lucas A M
authors: LA
authors: Ferreira
authors: Valbert N
authors: VN
authors: Cardoso
authors: Geovanni D
authors: GD
authors: Cassali
authors: Carlos E
authors: CE
authors: Salas
authors: Miriam T P
authors: MT
authors: Lopes
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D029441:Carica / Q000201:enzymology; D003546:Cysteine Endopeptidases / Q000494:pharmacology* / Q000627:therapeutic use; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005782:Gels; D006023:Glycoproteins / Q000494:pharmacology* / Q000627:therapeutic use; D007840:Latex / Q000737:chemistry*; D008297:Male; D051379:Mice; D008812:Mice, Hairless; D008517:Phytotherapy; D012867:Skin / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology*
chemical_list: D003911:Dextrans; D005782:Gels; D006023:Glycoproteins; D007840:Latex; C025614:sephadex; C090775:Carica candamarencis glycosylated cysteine proteinase; D003546:Cysteine Endopeptidases