title: [Mechanism of cinnamic aldehyde-inducing apoptosis of chronic myeloid leukemic cells in vitro].
abstract: The aim of this study was to investigate the apoptosis-inducing effect of cinnamic aldehyde (CA) on chronic myeloid leukemic (CML) cells and its mechanism. K562 cells and primary bone marrow mononuclear cells (MNC) from patients with CML were treated by various concentrations of CA. Flow cytometry was employed to measure the apoptosis of K562 cells and primary CML bone marrow MNC. Western blot was used to determine the expression of C-MYC and the phosphorylation of CrkL in K562 cells, and real-time polymerase chain reaction (real-time PCR) was used to quantify the expression of BCR-ABL mRNA in K562 cells. The results indicated that CA induced the apoptosis of K562 cells in a time- and dose-dependent manner. CA induced apoptosis of CML MNC dose-dependently. CA inhibited the expression of BCR-ABL mRNA and C-MYC, reduced CrkL phosphorylation levels in K562 cells. It is concluded that CA induces apoptosis of CML cells in vitro. Down-regulation of the expression and function of BCR-ABL may be one of its most important anti-leukemia mechanisms.
authors: Department of Hematology, Shenzhen Sixth People Hospital (Nanshan Hospital), Shenzhen 518052, Guangdong Province, China.
authors: Li-Qiong
authors: LQ
authors: Liu
authors: Ze-Lin
authors: ZL
authors: Liu
authors: Xin
authors: X
authors: Wang
authors: Hai-Yan
authors: HY
authors: Cui
authors: Meng-Di
authors: MD
authors: Jin
authors: Dan-Yu
authors: DY
authors: Wang
authors: Shi-Ang
authors: SA
authors: Huang
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology*
chemical_list: D000171:Acrolein; D016044:Fusion Proteins, bcr-abl; C012843:cinnamaldehyde