title: High-performance liquid chromatographic assay for the determination of nilotinib in human plasma.
abstract: A precise and convenient high-performance liquid chromatography (HPLC) method has been established to assay nilotinib in human plasma. Chromatographic separation of nilotinib was performed on a LiChrosphere(®)100 RP-18(e) column (250 mm×4.0 mm, 5 µm) using a mixture of acetonitrile and 0.01 M phosphate buffer (pH 3.0) (42 : 58, v/v) under isocratic conditions at a flow rate of 1.0 ml/min with ultraviolet (UV) detection at 266 nm. The calibration curve showed linearity at concentrations between 250 ng/ml and 5000 ng/ml (r(2)>0.999). The mean±S.D. absolute recovery of nilotinib from plasma was 99.2±3.3%. The coefficients of variation of both intra- and inter-day precision were below 9.1%. These results indicate that this new HPLC-based quantification may be useful for therapeutic drug monitoring of nilotinib to help manage treatment in patients with chronic myeloid leukemia in clinical practice.
authors: Department of Clinical Pharmaceutical Sciences, School of Pharmaceutical Sciences, Kumamoto University, 5–1 Oehonmachi, Kumamoto 862–0973, Japan.
authors: Misato
authors: M
authors: Yuki
authors: Yuji
authors: Y
authors: Yamakawa
authors: Takashi
authors: T
authors: Uchida
authors: Takeru
authors: T
authors: Nambu
authors: Tatsuya
authors: T
authors: Kawaguchi
authors: Akinobu
authors: A
authors: Hamada
authors: Hideyuki
authors: H
authors: Saito
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D047428:Protein Kinase Inhibitors / Q000097:blood*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D011743:Pyrimidines / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D047428:Protein Kinase Inhibitors; D011743:Pyrimidines; D011505:Protein-Tyrosine Kinases; C498826:nilotinib