title: Human fallopian tube mesenchymal stromal cells enhance bone regeneration in a xenotransplanted model.
abstract: We have recently reported that human fallopian tubes, which are discarded during surgical procedures of women submitted to sterilization or hysterectomies, are a rich source of human fallopian tube mesenchymal stromal cells (htMSCs). It has been previously shown that human mesenchymal stromal cells may be useful in enhancing the speed of bone regeneration. This prompted us to investigate whether htMSCs might be useful for the treatment of osteoporosis or other bone diseases, since they present a pronounced capacity for osteogenic differentiation in vitro. Based on this prior knowledge, our aim was to evaluate, in vivo, the osteogenic capacity of htMSCs to regenerate bone through an already described xenotransplantation model: nonimmunosuppressed (NIS) rats with cranial defects. htMSCs were obtained from five 30-50 years old healthy women and characterized by flow cytometry and for their multipotenciality in vitro capacity (osteogenic, chondrogenic and adipogenic differentiations). Two symmetric full-thickness cranial defects on each parietal region of seven NIS rats were performed. The left side (LS) of six animals was covered with CellCeram (Scaffdex)-a bioabsorbable ceramic composite scaffold that contains 60% hydroxyapatite and 40% β-tricalciumphosphate-only, and the right side (RS) with the CellCeram and htMSCs (10(6) cells/scaffold). The animals were euthanized at 30, 60 and 90 days postoperatively and cranial tissue samples were taken for histological analysis. After 90 days we observed neobone formation in both sides. However, in animals euthanized 30 and 60 days after the procedure, a mature bone was observed only on the side with htMSCs. PCR and immunofluorescence analysis confirmed the presence of human DNA and thus that human cells were not rejected, which further supports the imunomodulatory property of htMSCs. In conclusion, htMSCs can be used successfully to enhance bone regeneration in vivo, opening a new field for future treatments of osteoporosis and bone reconstruction.
authors: Human Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, Brazil.
authors: Tatiana
authors: T
authors: Jazedje
authors: Daniela F
authors: DF
authors: Bueno
authors: Bruno V P
authors: BV
authors: Almada
authors: Heloisa
authors: H
authors: Caetano
authors: Carlos E
authors: CE
authors: Czeresnia
authors: Paulo M
authors: PM
authors: Perin
authors: Silvio
authors: S
authors: Halpern
authors: Mariangela
authors: M
authors: Maluf
authors: Lucila P
authors: LP
authors: Evangelista
authors: Marcelo G
authors: MG
authors: Nisenbaum
authors: Marília T
authors: MT
authors: Martins
authors: Maria R
authors: MR
authors: Passos-Bueno
authors: Mayana
authors: M
authors: Zatz
mesh_terms: D000328:Adult; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002454:Cell Differentiation; D019070:Cell Lineage; D004247:DNA / Q000378:metabolism; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008875:Middle Aged; D008954:Models, Biological; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous
chemical_list: D004247:DNA