title: Detection of neural stem cells function in rats with traumatic brain injury by manganese-enhanced magnetic resonance imaging.
abstract: BACKGROUND
  Previously we had successfully tracked adult human neural stem cells (NSCs) labeled with superparamagnetic iron oxide particles (SPIOs) in host human brain after transplantation in vivo non-invasively by magnetic resonance imaging (MRI). However, the function of the transplanted NSCs could not be evaluated by the method. In the study, we applied manganese-enhanced MRI (ME-MRI) to detect NSCs function after implantation in brain of rats with traumatic brain injury (TBI) in vivo.
  
  
  METHODS
  Totally 40 TBI rats were randomly divided into 4 groups with 10 rats in each group. In group 1, the TBI rats did not receive NSCs transplantation. MnCl2·4H2O was intravenously injected, hyperosmolar mannitol was delivered to disrupt rightside blood brain barrier, and its contralateral forepaw was electrically stimulated. In group 2, the TBI rats received NSCs (labeled with SPIO) transplantation, and the ME-MRI procedure was same to group 1. In group 3, the TBI rats received NSCs (labeled with SPIO) transplantation, and the ME-MRI procedure was same to group 1, but diltiazem was introduced during the electrical stimulation period. In group 4, the TBI rats received phosphate buffered saline (PBS) injection, and the ME-MRI procedure was same to group 1.
  
  
  RESULTS
  Hyperintense signals were detected by ME-MRI in the cortex areas associated with somatosensory in TBI rats of group 2. These signals, which could not be induced in TBI rats of groups 1 and 4, disappeared when diltiazem was introduced in TBI rats of group 3.
  
  
  CONCLUSIONS
  In this initial study, we mapped implanted NSCs activity and its functional participation within local brain area in TBI rats by ME-MRI technique, paving the way for further pre-clinical research.
authors: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors: Hai-Liang
authors: HL
authors: Tang
authors: Hua-Ping
authors: HP
authors: Sun
authors: Xing
authors: X
authors: Wu
authors: Hong-Ying
authors: HY
authors: Sha
authors: Xiao-Yuan
authors: XY
authors: Feng
authors: Jian-Hong
authors: JH
authors: Zhu
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology / Q000601:surgery*; D002465:Cell Movement; D007089:Image Enhancement; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008345:Manganese; D058953:Neural Stem Cells / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008345:Manganese