title: p2 of rice stripe virus (RSV) interacts with OsSGS3 and is a silencing suppressor.
abstract: A rice cDNA library was screened by a galactosidase 4 (Gal4)-based yeast two-hybrid system with Rice stripe virus (RSV) p2 as bait. The results revealed that RSV p2 interacted with a rice protein exhibiting a high degree of identity with Arabidopsis thaliana suppressor of gene silencing 3 (AtSGS3). The interaction was confirmed by bimolecular fluorescence complementation assay. SGS3 has been shown to be involved in sense transgene-induced RNA silencing and in the biogenesis of trans-acting small interfering RNAs (ta-siRNAs), possibly functioning as a cofactor of RNA-dependent RNA polymerase 6 (RDR6). Given the intimate relationships between virus and RNA silencing, further experiments were conducted to show that p2 was a silencing suppressor. In addition, p2 enhanced the accumulation and pathogenicity of Potato virus X in Nicotiana benthamiana. Five genes that have been demonstrated to be targets of TAS3-derived ta-siRNAs were up-regulated in RSV-infected rice. The implications of these findings are discussed.
authors: Institute of Plant Virology, Fujian Agriculture and Forestry University, Key Laboratory of Plant Virology of Fujian Province, Fuzhou, Fujian, 350002, China.
authors: Zhenguo
authors: Z
authors: Du
authors: Donglai
authors: D
authors: Xiao
authors: Jianguo
authors: J
authors: Wu
authors: Dongsheng
authors: D
authors: Jia
authors: Zhengjie
authors: Z
authors: Yuan
authors: Ying
authors: Y
authors: Liu
authors: Liuyang
authors: L
authors: Hu
authors: Zhao
authors: Z
authors: Han
authors: Taiyun
authors: T
authors: Wei
authors: Qiying
authors: Q
authors: Lin
authors: Zujian
authors: Z
authors: Wu
authors: Lianhui
authors: L
authors: Xie
mesh_terms: D000595:Amino Acid Sequence; D015723:Gene Library; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D029364:Tenuivirus / Q000235:genetics / Q000472:pathogenicity*; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins