title: Intercellular production of tamavidin 1, a biotin-binding protein from Tamogitake mushroom, confers resistance to the blast fungus Magnaporthe oryzae in transgenic rice.
abstract: The blast fungus Magnaporthe oryzae, one of the most devastating rice pathogens in the world, shows biotin-dependent growth. We have developed a strategy for creating disease resistance to M. oryzae whereby intercellular production of tamavidin 1, a biotin-binding protein from Pleurotus cornucopiae occurs in transgenic rice plants. The gene that encodes tamavidin 1, fused to the sequence for a secretion signal peptide derived from rice chitinase gene, was connected to the Cauliflower mosaic virus 35S promoter, and the resultant construct was introduced into rice. The tamavidin 1 was accumulated at levels of 0.1-0.2% of total soluble leaf proteins in the transgenic rice and it was localized in the intercellular space of rice leaves. The tamavidin 1 purified from the transgenic rice was active, it bound to biotin and inhibited in vitro growth of M. oryzae by causing biotin deficiency. The transgenic rice plants showed a significant resistance to M. oryzae. This study shows the possibility of a new strategy to engineer disease resistance in higher plants by taking advantage of a pathogen's auxotrophy.
authors: Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan. yoshimitsu.takakura@jt.com
authors: Yoshimitsu
authors: Y
authors: Takakura
authors: Naomi
authors: N
authors: Oka
authors: Junko
authors: J
authors: Suzuki
authors: Hiroshi
authors: H
authors: Tsukamoto
authors: Yuji
authors: Y
authors: Ishida
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D000595:Amino Acid Sequence; D001710:Biotin / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry; D060467:Disease Resistance / Q000276:immunology*; D005110:Extracellular Space / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry; D015151:Immunoblotting; D020082:Magnaporthe / Q000502:physiology*; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology*; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; C017071:biotin-binding proteins; D001710:Biotin