title: Understanding the allosteric trigger for the fructose-1,6-bisphosphate regulation of the ADP-glucose pyrophosphorylase from Escherichia coli.
abstract: ADP-glucose pyrophosphorylase is the enzyme responsible for the regulation of glycogen synthesis in bacteria. The enzyme N-terminal domain has a Rossmann-like fold with three neighbor loops facing the substrate ATP. In the Escherichia coli enzyme, one of those loops also faces the regulatory site containing Lys(39), a residue involved in binding of the allosteric activator fructose-1,6-bisphosphate and its analog pyridoxal-phosphate. The other two loops contain Trp(113) and Gln(74), respectively, which are highly conserved among all the ADP-glucose pyrophosphorylases. Molecular modeling of the E. coli enzyme showed that binding of ATP correlates with conformational changes of the latter two loops, going from an open to a closed (substrate-bound) form. Alanine mutants of Trp(113) or Gln(74) did not change apparent affinities for the substrates, but they became insensitive to activation by fructose-1,6-bisphosphate. By capillary electrophoresis we found that the mutant enzymes still bind fructose-1,6-bisphosphate, with similar affinity as the wild type enzyme. Since the mutations did not alter binding of the activator, they must have disrupted the communication between the regulatory and the substrate sites. This agrees with a regulatory mechanism where the interaction with the allosteric activator triggers conformational changes at the level of loops containing residues Trp(113) and Gln(74).
authors: Laboratorio de Enzimología Molecular, Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Paraje El Pozo CC 242, S3000ZAA Santa Fe, Argentina.
authors: Carlos M
authors: CM
authors: Figueroa
authors: María C
authors: MC
authors: Esper
authors: Ana
authors: A
authors: Bertolo
authors: Ana M
authors: AM
authors: Demonte
authors: Mabel
authors: M
authors: Aleanzi
authors: Alberto A
authors: AA
authors: Iglesias
authors: Miguel A
authors: MA
authors: Ballicora
mesh_terms: D000494:Allosteric Regulation; D019075:Electrophoresis, Capillary; D004926:Escherichia coli / Q000201:enzymology*; D005635:Fructosediphosphates / Q000378:metabolism*; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed
chemical_list: D005635:Fructosediphosphates; D051599:Glucose-1-Phosphate Adenylyltransferase; C029063:fructose-1,6-diphosphate