title: The PmrA/PmrB regulatory system controls the expression of the wzzfepE gene involved in the O-antigen synthesis of Salmonella enterica serovar Typhimurium.
abstract: The degree of polymerization of O-antigen from Salmonella enterica serovar Typhimurium is controlled by the products of the wzz(s)(t) and wzz(fepE) genes. In the present study we investigated the role of the PmrA/PmrB regulatory system in wzz(fepE) transcription. We report that the direct binding of the PmrA regulator to a specific promoter site induces the expression of the wzz(fepE) gene. This effect increases the amount of very long (VL) O-antigen, which is required for the resistance of Salmonella to serum human complement and polymyxin B, and for the replication of the bacteria within macrophages. The results obtained here highlight functional differences between Wzz(fepE) and Wzz(st), although the genes for both proteins are regulated in a PmrA-dependent way.
authors: Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Tucumán) and Instituto de Química Biologica 'Dr Bernabe Bloj', Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
authors: María de Las Mercedes
authors: MLM
authors: Pescaretti
authors: Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Tucumán) and Instituto de Química Biologica 'Dr Bernabe Bloj', Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
authors: Fabián E
authors: FE
authors: López
authors: Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Tucumán) and Instituto de Química Biologica 'Dr Bernabe Bloj', Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
authors: Roberto D
authors: RD
authors: Morero
authors: Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Tucumán) and Instituto de Química Biologica 'Dr Bernabe Bloj', Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
authors: Mónica A
authors: MA
authors: Delgado
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D019081:O Antigens / Q000096:biosynthesis*; D011112:Polymyxin B / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D019081:O Antigens; C409039:PmrB protein, bacteria; D012333:RNA, Messenger; D014157:Transcription Factors; C081902:pmrA protein, Bacteria; C489587:rol protein, Salmonella typhimurium; D011112:Polymyxin B