title: Transportin-SR is required for proper splicing of resistance genes and plant immunity.
abstract: Transportin-SR (TRN-SR) is a member of the importin-β super-family that functions as the nuclear import receptor for serine-arginine rich (SR) proteins, which play diverse roles in RNA metabolism. Here we report the identification and cloning of mos14 (modifier of snc1-1, 14), a mutation that suppresses the immune responses conditioned by the auto-activated Resistance (R) protein snc1 (suppressor of npr1-1, constitutive 1). MOS14 encodes a nuclear protein with high similarity to previously characterized TRN-SR proteins in animals. Yeast two-hybrid assays showed that MOS14 interacts with AtRAN1 via its N-terminus and SR proteins via its C-terminus. In mos14-1, localization of several SR proteins to the nucleus was impaired, confirming that MOS14 functions as a TRN-SR. The mos14-1 mutation results in altered splicing patterns of SNC1 and another R gene RPS4 and compromised resistance mediated by snc1 and RPS4, suggesting that nuclear import of SR proteins by MOS14 is required for proper splicing of these two R genes and is important for their functions in plant immunity.
authors: Graduate Program in Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors: Shaohua
authors: S
authors: Xu
authors: Zhibin
authors: Z
authors: Zhang
authors: Beibei
authors: B
authors: Jing
authors: Patrick
authors: P
authors: Gannon
authors: Jinmei
authors: J
authors: Ding
authors: Fang
authors: F
authors: Xu
authors: Xin
authors: X
authors: Li
authors: Yuelin
authors: Y
authors: Zhang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D017343:Genes, Plant / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D057865:Plant Immunity / Q000235:genetics*; D011485:Protein Binding / Q000502:physiology; D021381:Protein Transport / Q000235:genetics; D012326:RNA Splicing / Q000235:genetics*; D028961:beta Karyopherins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D028961:beta Karyopherins; C119888:transportin SR