title: Using the Dunn chemotaxis chamber to analyze primary cell migration in real time.
abstract: The directed migration of cells (chemotaxis) occurs not only during wound healing and inflammatory responses but also during embryonic development. However, the intracellular signaling pathways that enable a cell to detect a chemoattractant and subsequently migrate toward the source are not clearly defined. The Dunn chemotaxis chamber in conjunction with time-lapse microscopy is a powerful tool that enables the user to observe directly the morphological response of cells to a chemoattractant in real time. Here, using the Dunn chemotaxis chamber, we describe the response of murine bone marrow-derived macrophages to colony stimulating factor-1. This is a particularly useful protocol as it can be adapted to study bone marrow-derived macrophages isolated from genetically modified mice and thus study the requirement of a specific protein in cell migration and chemotaxis.
authors: Division of Cancer Studies, King’s College London, London, UK.
authors: Sanjay
authors: S
authors: Chaubey
authors: Anne J
authors: AJ
authors: Ridley
authors: Claire M
authors: CM
authors: Wells
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D054443:Cell Migration Assays / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000379:methods; D002633:Chemotaxis; D008264:Macrophages / Q000166:cytology / Q000502:physiology; D051379:Mice; D059008:Time-Lapse Imaging / Q000295:instrumentation* / Q000379:methods