title: Osteogenic potential of cells derived from nasal septum.
abstract: BACKGROUND
  The research addressed to detect new molecular targets in the development of therapeutic strategies aimed to repair bone tissues. The AIM OF THIS STUDY was to determine the potential osteogenic activity of bone cells from the nasal septum and their use to perform accurate molecular analysis from a single sample.
  
  
  METHODS
  The cells, after nasal septum surgery, were subjected to gene silencing, Reverse Transcriptase - Polymerase Chain reactions, immunocytochemistry and chromatin immunoprecipitation.
  
  
  RESULTS
  Cells from the nasal septum can give rise to mature osteoblasts that express osteogenic markers (ALP, Runx2, Slug) and are able to mineralize. We demonstrated that Runx2, a transcription factor critical in early osteospecific differentiation, interacts in vivo with the promoter of the SLUG gene, a marker of osteoblast maturation.
  
  
  CONCLUSIONS
  We demonstrated that nasal septum-derived osteoblasts represent an interesting alternative source for bone forming cells, and a promising material to be utilized in bone cellular therapy.
authors: Department of Biochemistry and Molecular Biology, Section of Molecular Biology, University of Ferrara, Ferrara, Italy.
authors: E
authors: E
authors: Torreggiani
authors: C
authors: C
authors: Bianchini
authors: L
authors: L
authors: Penolazzi
authors: E
authors: E
authors: Lambertini
authors: R
authors: R
authors: Vecchiatini
authors: A
authors: A
authors: Canella
authors: R
authors: R
authors: Gambari
authors: E
authors: E
authors: Magri
authors: S
authors: S
authors: Pelucchi
authors: A
authors: A
authors: Pastore
authors: R
authors: R
authors: Piva
mesh_terms: D000328:Adult; D000368:Aged; D047369:Chromatin Immunoprecipitation; D050659:Core Binding Factor Alpha 1 Subunit; D005260:Female; D005434:Flow Cytometry; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009300:Nasal Septum / Q000166:cytology*; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071250:Snail Family Transcription Factors; D023822:Tissue Engineering / Q000379:methods*; D014157:Transcription Factors; D014162:Transfection
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D034741:RNA, Small Interfering; C493709:RUNX2 protein, human; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors