title: Validity and reliability of perinatal biomarkers of adiposity after storage as dried blood spots on paper.
abstract: OBJECTIVE
  To validate use of chip-based immunoaffinity capillary electrophoresis on dried blood spot samples (DBSS) to measure obesity-related hormones.
  
  
  METHODS
  Chip-based immunoaffinity capillary electrophoresis was used to measure adiponectin, leptin and insulin in capillary serum and DBSS in pregnant women and infant heelstick at birth and six weeks. Concordance of measurements was determined with Pearson's correlation and Bland-Altman plots.
  
  
  RESULTS
  We report high concordance between results obtained from serum and DBSS.
  
  
  CONCLUSIONS
  Ease of sample collection and storage makes DBSS an optimal method for use in studies involving neonates and young children, as well as studies conducted in areas where freezer storage is not available.
authors: Department of Pediatrics, University of Utah, Salt Lake City, Utah 84158, USA. Nicole.mihalopoulos@hsc.utah.edu
authors: Nicole L
authors: NL
authors: Mihalopoulos
authors: Terry M
authors: TM
authors: Phillips
authors: Hillarie
authors: H
authors: Slater
authors: J Anne
authors: JA
authors: Thomson
authors: Michael W
authors: MW
authors: Varner
authors: M Nazeem
authors: MN
authors: Nanjee
authors: Laurie J
authors: LJ
authors: Moyer-Mileur
mesh_terms: D052242:Adiponectin / Q000097:blood*; D050154:Adiposity; D015415:Biomarkers / Q000097:blood; D002196:Capillaries / Q000737:chemistry; D059788:Dried Blood Spot Testing / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007328:Insulin / Q000097:blood*; D020738:Leptin / Q000097:blood*; D009765:Obesity / Q000175:diagnosis; D011247:Pregnancy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D052242:Adiponectin; D015415:Biomarkers; D007328:Insulin; D020738:Leptin