title: LC-MS/MS quantification of sulforaphane and indole-3-carbinol metabolites in human plasma and urine after dietary intake of selenium-fortified broccoli.
abstract: This study aimed at developing a sensitive LC-MS/MS method for the quantification of sulforaphane (SFN) and indole-3-carbinol metabolites in plasma and urine after dietary intake of regular and selenium-fertilized broccoli using stable isotope dilution analysis. In a three-armed, placebo-controlled, randomized human intervention study with 76 healthy volunteers, 200 g of regular (485 μg of total glucosinolates and <0.01 μg of selenium per gram fresh weight) or selenium-fertilized broccoli (589 μg of total glucosinolates and 0.25 μg of selenium per gram fresh weight) was administered daily for 4 weeks. Glucoraphanin and glucobrassicin metabolites quantified in plasma and urine were SFN-glutathione, SFN-cysteine, SFN-cysteinylglycine, SFN-acetylcysteine, and indole-3-carboxaldehyde, indole-3-carboxylic acid, and ascorbigen, respectively. Dietary intake of selenium-fertilized broccoli increased serum selenium concentration analyzed by means of atomic absorption spectroscopy by up to 25% (p < 0.001), but affected neither glucosinolate concentrations in broccoli nor their metabolite concentrations in plasma and urine compared to regular broccoli.
authors: German Research Center for Food Chemistry, Freising, Germany.
authors: Johanna
authors: J
authors: Hauder
authors: Stefanie
authors: S
authors: Winkler
authors: Achim
authors: A
authors: Bub
authors: Corinna E
authors: CE
authors: Rüfer
authors: Marc
authors: M
authors: Pignitter
authors: Veronika
authors: V
authors: Somoza
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001937:Brassica / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004032:Diet; D005260:Female; D005308:Fertilizers / Q000032:analysis; D005961:Glucosinolates / Q000378:metabolism; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D017879:Isothiocyanates; D008297:Male; D008875:Middle Aged; D012643:Selenium / Q000032:analysis / Q000378:metabolism*; D013454:Sulfoxides; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013861:Thiocyanates / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D005308:Fertilizers; D005961:Glucosinolates; D007211:Indoles; D017879:Isothiocyanates; D013454:Sulfoxides; D013861:Thiocyanates; C016517:indole-3-carbinol; C048308:glucobrassicin; C016766:sulforaphane; D012643:Selenium