title: Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37.
abstract: Ankyrin repeat domain 37 (Ankrd37), a protein containing ankyrin repeats (ARs) and a putative nuclear localization signal (NLS), is highly conserved from zebrafish to humans. In mouse testes, Ankrd37 protein was initially present in the cytoplasm of elongating spermatids, and finally restricted to the nuclei of spermatozoa during spermatogenesis. Ankrd37 bound to feminization 1 homolog b (Fem1b) as indicated by yeast two-hybrid screening and co-immunoprecipitation assays. Ankrd37 facilitated the transport of Fem1b protein from cytoplasm to nuclei in co-transfected CHO cells. In addition, the protein level of Ankrd37 was decreased in a Fem1b dose-dependent manner as shown by the transfection experiments, and Ankrd37 was ubiquitinated in the presence of Fem1b. As the nematode Fem-1 has been shown to target its downstream effector TRA-1 for ubiquitin-mediated degradation, we report in the present study that mouse Fem1b targets Ankrd37 for degradation in the same manner.
authors: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors: Yu-Qiang
authors: YQ
authors: Shi
authors: Shang-Ying
authors: SY
authors: Liao
authors: Xin-Jie
authors: XJ
authors: Zhuang
authors: Chun-Sheng
authors: CS
authors: Han
mesh_terms: D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics*; D015153:Blotting, Western; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D019913:Nuclear Localization Signals; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes; D054875:Ubiquitination
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D019913:Nuclear Localization Signals; D025801:Ubiquitin; C498596:Fem1b protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes