title: Phylogenetic analysis of hepatitis B virus genotype F complete genome sequences from Chilean patients with chronic infection.
abstract: Molecular epidemiological data concerning the hepatitis B virus (HBV) in Chile are not known completely. Since the HBV genotype F is the most prevalent in the country, the goal of this study was to obtain full HBV genome sequences from patients infected chronically in order to determine their subgenotypes and the occurrence of resistance-associated mutations. Twenty-one serum samples from antiviral drug-naive patients with chronic hepatitis B were subjected to full-length PCR amplification, and both strands of the whole genomes were fully sequenced. Phylogenetic analyses were performed along with reference sequences available from GenBank (n = 290). The sequences were aligned using Clustal X and edited in the SE-AL software. Bayesian phylogenetic analyses were conducted by Markov Chain Monte Carlo simulations (MCMC) for 10 million generations in order to obtain the substitution tree using BEAST. The sequences were also analyzed for the presence of primary drug resistance mutations using CodonCode Aligner Software. The phylogenetic analyses indicated that all sequences were found to be the HBV subgenotype F1b, clustered into four different groups, suggesting that diverse lineages of this subgenotype may be circulating within this population of Chilean patients.
authors: Gastroenterology Section, Department of Medicine, University of Chile Clinical Hospital, Santiago, Chile. mvenegas@redclinicauchile.cl
authors: Mauricio
authors: M
authors: Venegas
authors: Mónica V
authors: MV
authors: Alvarado-Mora
authors: Rodrigo A
authors: RA
authors: Villanueva
authors: João R
authors: JR
authors: Rebello Pinho
authors: Flair J
authors: FJ
authors: Carrilho
authors: Stephen
authors: S
authors: Locarnini
authors: Lilly
authors: L
authors: Yuen
authors: Javier
authors: J
authors: Brahm
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002648:Child; D002677:Chile; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D024921:Drug Resistance, Multiple, Viral / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral