title: Coding vs non-coding: Translatability of short ORFs found in putative non-coding transcripts.
abstract: Genome analysis has identified a number of putative non-protein-coding transcripts that do not contain ORFs longer than 100 codons. Although evidence strongly suggests that non-coding RNAs are important in a variety of biological phenomena, the discovery of small peptide-coding mRNAs confirms that some transcripts that have been assumed to be non-coding actually have coding potential. Their abundance and importance in biological phenomena makes the sorting of non-coding RNAs from small peptide-coding mRNAs a key issue in functional genomics. However, validating the coding potential of small peptide-coding RNAs is complicated, because their ORF sequences are usually too short for computational analysis. In this review, we discuss computational and experimental methods for validating the translatability of these non-coding RNAs.
authors: Okazaki Institute for Integrated Biosciences, National Institutes of Natural Sciences, 5-1 Myodaiji-Higashiyama, Okazaki, Aichi 444-8787, Japan. kageyama@nibb.ac.jp
authors: Yuji
authors: Y
authors: Kageyama
authors: Takefumi
authors: T
authors: Kondo
authors: Yoshiko
authors: Y
authors: Hashimoto
mesh_terms: D017398:Alternative Splicing / Q000235:genetics; D000818:Animals; D019295:Computational Biology; D005786:Gene Expression Regulation; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics*; D014176:Protein Biosynthesis / Q000235:genetics*; D059368:RNA Isoforms / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D059368:RNA Isoforms; D022661:RNA, Untranslated