title: Aberrant production of soluble co-stimulatory molecules CTLA-4 and CD28 in patients with chronic hepatitis B.
abstract: Co-stimulation signals are critical for anti-viral immunity. Here we investigated whether there is altered expression and the clinical significance of soluble co-stimulatory molecules in patients with chronic HBV infection. Serum concentrations and ex vivo production of soluble CTLA-4, CD28, CD80 and CD86 were analyzed by enzyme-linked immunosorbent assay. Serum sCTLA-4 and sCD28 concentrations in all chronic HBV patients were significantly higher than concentrations in healthy control subjects. Serum sCTLA-4 and sCD28 correlated significantly with alanine aminotransferase in all chronic HBV patients. Upon mitogen stimulation of peripheral blood mononuclear cells, the percentage increases in ex vivo production of sCD28 and sCD80 and the percentage decrease in sCTLA-4 release were all significantly lower in chronic HBV patients than those in healthy subjects. The aberrant production of soluble co-stimulatory molecules should reflect the dysregulation of T cell activation and is related to the pathogenesis and severity of chronic HBV infection.
authors: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
authors: Ju
authors: J
authors: Cao
authors: Liping
authors: L
authors: Zhang
authors: Shifeng
authors: S
authors: Huang
authors: Pu
authors: P
authors: Chen
authors: Lin
authors: L
authors: Zou
authors: Hui
authors: H
authors: Chen
authors: Yu
authors: Y
authors: Xiang
authors: Xiaofei
authors: X
authors: Lai
authors: Guosheng
authors: G
authors: Ren
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D051940:B7-2 Antigen / Q000097:blood / Q000378:metabolism; D018106:CD28 Antigens / Q000097:blood* / Q000378:metabolism*; D060908:CTLA-4 Antigen / Q000097:blood* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019694:Hepatitis B, Chronic / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D051940:B7-2 Antigen; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D000410:Alanine Transaminase