title: Constant transmission of mitochondrial DNA in intergeneric cloned embryos reconstructed from swamp buffalo fibroblasts and bovine ooplasm.
abstract: Although interspecies/intergeneric somatic cell nuclear transfer (iSCNT) has been proposed as a tool to produce offspring of endangered species, conflict between donor nucleus and recipient cytoplasm in iSCNT embryos has been identified as an impediment to implementation for agricultural production. To investigate the nuclear-mitochondrial interactions on the developmental potential of iSCNT embryos, we analyzed the mtDNA copy numbers in iSCNT embryos reconstructed with water buffalo (swamp type) fibroblasts and bovine enucleated oocytes (buffalo iSCNT). As controls, SCNT embryos were derived from bovine fibroblasts (bovine SCNT). Buffalo iSCNT and bovine SCNT embryos showed similar rates of cleavage and development to the 8-cell stage (P>0.05). However, buffalo iSCNT embryos did not develop beyond the 16-cell stage. Both bovine and buffalo mtDNA content in buffalo iSCNT embryos was stable throughout the nuclear transfer process, and arrested at the 8- to 16-cell stage (P>0.05). In bovine SCNT embryos that developed to the blastocyst stage, mtDNA copy number was increased (P<0.05). In conclusion, both the donor cell and recipient cytoplast mtDNAs of buffalo iSCNT embryos were identified and maintained through the iSCNT process until the 8-16-cell stage. In addition, the copy number of mtDNA per embryo was a useful monitor to investigate nuclear-mitochondrial interactions.
authors: Embryo Technology and Stem Cell Research Center and School of Biotechnology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
authors: Kanokwan
authors: K
authors: Srirattana
authors: Kazutsugu
authors: K
authors: Matsukawa
authors: Satoshi
authors: S
authors: Akagi
authors: Mariko
authors: M
authors: Tasai
authors: Takahiro
authors: T
authors: Tagami
authors: Keijiro
authors: K
authors: Nirasawa
authors: Takashi
authors: T
authors: Nagai
authors: Yukio
authors: Y
authors: Kanai
authors: Rangsun
authors: R
authors: Parnpai
authors: Kumiko
authors: K
authors: Takeda
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002467:Cell Nucleus / Q000502:physiology; D019976:Cloning, Organism; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial / Q000032:analysis*; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology; D047108:Embryonic Development; D005347:Fibroblasts / Q000502:physiology; D053652:Nuclear Transfer Techniques
chemical_list: D021901:DNA, Intergenic; D004272:DNA, Mitochondrial