title: Heparin-like glycosaminoglycan/amine salt-bridge interactions: a new potential tool for HLGAGs analysis using mass spectrometry.
abstract: Characterization of glycosaminoglycans poses a challenge for current analytical techniques, as they are highly acidic, polydisperse and heterogeneous compounds. The purpose of this study is the separation and analysis of a partially depolymerized heparin-like glycosaminoglycan by on-line ion-pairing reversed-phase high-performance liquid chromatography/electrospray mass spectrometry. The gas-phase behavior of two synthesized glycosaminoglycans has been investigated. Dibutylamine was found to be the best suited ion-pairing reagents for mass spectrometry analysis. The optimized ion-pairing conditions provide reproducible and easily interpretable electrospray mass spectra in both negative and positive ESI modes. The glycosaminoglycans are detected as a non-covalent complex with amines. In fact, the observed ionic species and their gas-phase dissociation under CID conditions revealed the presence of salt bridge interactions in the gas phase.
authors: Laboratoire de Chimie Structurale Organique et Biologique - UMR 7201, University of Paris VI, Batiment F, 7éme étage, boîte 45, 4 Place Jussieu, F75252 Paris Cedex 05, France.
authors: Baiyi
authors: B
authors: Xue
authors: Sandra
authors: S
authors: Alves
authors: Coraline
authors: C
authors: Desbans
authors: Marie
authors: M
authors: Souchaud
authors: Aziz
authors: A
authors: Filali-Ansary
authors: Patrick
authors: P
authors: Soubayrol
authors: Jean-Claude
authors: JC
authors: Tabet
mesh_terms: D002082:Butylamines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006025:Glycosaminoglycans / Q000737:chemistry* / Q000302:isolation & purification; D006493:Heparin / Q000737:chemistry*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D002082:Butylamines; D006025:Glycosaminoglycans; C045274:dibutylamine; D006493:Heparin