title: Myeloid lineage cells inhibit neurite outgrowth through a myosin II-dependent mechanism.
abstract: The molecular mechanisms that underlie the axonal damage that accompanies CNS inflammation are largely unknown. Here, we investigate the effects of immune cells on neuronal viability and axonal growth and show that conditioned media from myeloid lineage cells inhibit neurite outgrowth without causing apoptosis. Treatment with monocyte conditioned medium enhances myosin light chain phosphorylation in neurons and the neurite outgrowth inhibitory effect of myeloid lineage cells can be attenuated with the myosin II inhibitor blebbistatin. Our results suggest that in the context of CNS inflammation myeloid cells may limit axonal repair in the CNS via a myosin II-dependent mechanism.
authors: Department of Neurology and Neurosurgery, Montreal Neurological Institute, 3801 Rue University, Montreal, Quebec, Canada H3A 2B4.
authors: Madeline
authors: M
authors: Pool
authors: Isabel
authors: I
authors: Rambaldi
authors: Bryce A
authors: BA
authors: Durafourt
authors: Melissa C
authors: MC
authors: Wright
authors: Jack P
authors: JP
authors: Antel
authors: Amit
authors: A
authors: Bar-Or
authors: Alyson E
authors: AE
authors: Fournier
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation / Q000276:immunology*; D019070:Cell Lineage / Q000276:immunology; D002478:Cells, Cultured; D006131:Growth Inhibitors / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D022423:Myeloid Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016501:Neurites / Q000276:immunology* / Q000473:pathology; D024501:Nonmuscle Myosin Type IIB / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006131:Growth Inhibitors; D024501:Nonmuscle Myosin Type IIB