title: Characterization of the transgenic rice event TT51-1 and construction of a reference plasmid.
abstract: Transgenic rice TT51-1 (BT63) is an insect resistant strain that was granted for safety certificate in China in 2009. This study characterizes the transgenic event TT51-1 using a GenomeWalker strategy. The organization of the transgenes indicated that the transgenes on two plasmids, pFHBT1 and pGL2RC7, had been integrated at the same locus. The sequence of the event TT51-1 spanned 8725 bp, including a truncated Cry1Ab/Ac cassette, an intact Cry1Ab/Ac cassette, two Amp gene segments, and an Hph gene segment. The 5' and 3' plant flanking sequences were isolated and used to locate the transgenes to chromosome 10 in TT51-1. The isolated TT51-1 fragment and a fragment of the rice PLD gene were integrated into a plasmid vector, to create plasmid pK-TT51 as a calibrator for detecting rice containing TT51-1. Analysis of unknown samples indicated that the reference plasmid was a reliable alternative to TT51-1 genomic DNA.
authors: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.
authors: Yinglong
authors: Y
authors: Cao
authors: Gang
authors: G
authors: Wu
authors: Yuhua
authors: Y
authors: Wu
authors: Shujing
authors: S
authors: Nie
authors: Li
authors: L
authors: Zhang
authors: Changming
authors: C
authors: Lu
mesh_terms: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D004731:Endotoxins / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D019076:Transgenes
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis