title: Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells.
abstract: Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
authors: Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors: Yun Soo
authors: YS
authors: Jang
authors: Kyoung-Ju
authors: KJ
authors: Song
authors: Ju Young
authors: JY
authors: Kim
authors: Young Ah
authors: YA
authors: Lee
authors: Kyeong Ah
authors: KA
authors: Kim
authors: Sang Kyou
authors: SK
authors: Lee
authors: Myeong Heon
authors: MH
authors: Shin
mesh_terms: D002154:Calpain / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004151:Dipeptides / Q000378:metabolism; D004748:Entamoeba histolytica / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000469:parasitology*; D055785:Gene Knockdown Techniques; D006801:Humans
chemical_list: D004151:Dipeptides; C071482:calpeptin; D002154:Calpain; C449000:m-calpain; C449002:mu-calpain