title: Aplysqualenol A binds to the light chain of dynein type 1 (DYNLL1).
abstract: A bidirectional affinity system has been developed for the identification of cancer-related natural products and their biological targets. Aplysqualenol A is thus selectively identified as a ligand of the dynein light chain. The use of forward and reverse affinity methods suggests that both small-molecule isolation and target identification can be conducted using conventional molecular biological methods.
authors: Department of Chemistry, University of Puerto Rico, UPR Station, San Juan, 00931-3346, USA.
authors: Brunilda
authors: B
authors: Vera
authors: Abimael D
authors: AD
authors: Rodríguez
authors: James J
authors: JJ
authors: La Clair
mesh_terms: D000595:Amino Acid Sequence; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D056445:Cytoplasmic Dyneins / Q000737:chemistry / Q000378:metabolism*; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D045325:HCT116 Cells; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011485:Protein Binding; D011714:Pyrans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D001688:Biological Products; D005663:Furans; D011714:Pyrans; C578578:aplysqualenol A; C427758:DYNLL1 protein, human; D056445:Cytoplasmic Dyneins