title: Microarray-based identification of transcription factor target genes.
abstract: Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.
authors: Molecular Genetics Group, Department of Biology, Faculty of Science, Utrecht University, Utrecht, The Netherlands. martieu@gmail.com
authors: Maartje
authors: M
authors: Gorte
authors: Anneke
authors: A
authors: Horstman
authors: Robert B
authors: RB
authors: Page
authors: Renze
authors: R
authors: Heidstra
authors: Arnold
authors: A
authors: Stromberg
authors: Kim
authors: K
authors: Boutilier
mesh_terms: D047369:Chromatin Immunoprecipitation / Q000379:methods; D004268:DNA-Binding Proteins; D005784:Gene Amplification; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D018749:RNA, Plant; D014157:Transcription Factors