title: Sensitive fluorescence assay of anthrax protective antigen with two new DNA aptamers and their binding properties.
abstract: A homogeneous assay of the protective antigen in anthrax toxin is reported using two new PA-specific aptamers for selective and sensitive detection, based on reduction in the fluorescence emission according to the formation of the aptamer-PA ternary complex. PA at 1 nM was readily detected using OliGreen as a fluorophore in HEPES buffer. We also demonstrated that the PA detection could be performed in blood serum. The binding interaction between the aptamer and PA was strong enough to dehybridize double-stranded DNA paired completely with 12 bases at room temperature. Moreover, this fluorescence study revealed that the binding sites of the two aptamers were located differently on the PA protein. We believe our approach may lay the groundwork for the real-time detection of PA.
authors: Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 120-750, Korea.
authors: Byul Nim
authors: BN
authors: Oh
authors: Sungeun
authors: S
authors: Lee
authors: Hye-Yeon
authors: HY
authors: Park
authors: Jin-Ook
authors: JO
authors: Baeg
authors: Moon-Young
authors: MY
authors: Yoon
authors: Jinheung
authors: J
authors: Kim
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry / Q000378:metabolism*; D003486:Cyanides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D020011:Protective Agents / Q000032:analysis*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000942:Antigens, Bacterial; D052157:Aptamers, Nucleotide; D001427:Bacterial Toxins; D003486:Cyanides; D005456:Fluorescent Dyes; D020011:Protective Agents; C030325:anthrax toxin