title: Early effects of portal flow modulation after extended liver resection in rat.
abstract: BACKGROUND
  The incidence of small-for-size-liver-syndrome after liver transplantation and extended liver resection may be reduced by portal flow modulation. However, many aspects of the small-for-size-liver-syndrome pathogenesis are still unclear. In this experimental study we evaluated the early effects of portal flow modulation after 80% hepatic resection in rats.
  
  
  METHODS
  Rats were randomised in: sham operation (G1), conventional hepatic resection (G2), splenectomy and hepatic resection (G3), splenic transposition followed by hepatic resection after three weeks (G4). Six hours after operation, oxygen saturation of hepatic vein blood, glutathione, and standard liver markers were measured from hepatic venous blood. Glutathione measurement and histopatological examination were performed in the remnant liver.
  
  
  RESULTS
  Total bilirubin and liver glutathione did not show differences between groups. Aspartate aminotransferase and alanine aminotransferase significantly increased in G2-G4 groups. Blood glutathione and oxygen saturation of hepatic vein blood were lower in G2 than in other groups. A gradient of micro-vesicular degeneration was more severe in G2 compared with G3 and G4. Apoptosis, hemorrhagic necrosis, mitochondrial damage and leucocyte adhesion were evident in G2.
  
  
  CONCLUSIONS
  The portal flow modulation induced by splenectomy or splenic transposition was effective in limiting early damage after extended liver resection.
authors: Department of General Surgery and Organ Transplantation, San Martino University Hospital, Genoa, Italy. didomenico.stefano@gmail.com
authors: Stefano
authors: S
authors: Di Domenico
authors: Gregorio
authors: G
authors: Santori
authors: Nicola
authors: N
authors: Traverso
authors: Emanuela
authors: E
authors: Balbis
authors: Annalisa
authors: A
authors: Furfaro
authors: Federica
authors: F
authors: Grillo
authors: Raffaella
authors: R
authors: Gentile
authors: Bruna
authors: B
authors: Bocca
authors: Maximiliano
authors: M
authors: Gelli
authors: Enzo
authors: E
authors: Andorno
authors: Ahmed
authors: A
authors: Dahame
authors: Damiano
authors: D
authors: Cottalasso
authors: Umberto
authors: U
authors: Valente
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D006498:Hepatectomy / Q000009:adverse effects*; D008099:Liver / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D008102:Liver Circulation / Q000502:physiology; D008297:Male; D009336:Necrosis; D009929:Organ Size / Q000502:physiology*; D010100:Oxygen / Q000097:blood; D011168:Portal System / Q000503:physiopathology*; D051381:Rats; D013156:Splenectomy
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D001663:Bilirubin; D010100:Oxygen