title: Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles.
abstract: Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials due to their antibacterial properties. In this study, we examined the effects of polyvinylpyrrolidone (PVP)-coated AgNPs (average size 2.3nm) on angiogenesis in both an in vivo model and an in vitro endothelial cell line, SVEC4-10. Increased angiogenesis was detected around the injection site of AgNP-containing Matrigel in vivo. AgNPs also increased the infiltration of endothelial cells and the hemoglobin (Hb) content in AgNP-Matrigel plugs implanted into mice. AgNPs induced endothelial cell tube formation on growth factor-reduced Matrigel, production of reactive oxygen species (ROS), and production of angiogenic factors, such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), in SVEC4-10 cells. In addition, AgNPs promoted the activation of FAK, Akt, ERK1/2, and p38, which are all involved in VEGF receptor (VEGFR)-mediated signaling. Finally, AgNP-treated tumors caused angiogenesis around tumors in B16F10 melanomas after they were injected into mice, and the Hb concentration in the tumors increased in a concentration-dependent manner with AgNP treatment. Thus, our study suggests that exposure to AgNPs can cause angiogenesis through the production of angiogenic factors.
authors: Department of Biological Science and the Research Center for Women's Disease, Sookmyung Women's University, Seoul, Republic of Korea.
authors: Kyeongah
authors: K
authors: Kang
authors: Dae-Hyoun
authors: DH
authors: Lim
authors: In-Hong
authors: IH
authors: Choi
authors: Taegyeong
authors: T
authors: Kang
authors: Kangtaek
authors: K
authors: Lee
authors: Eun-Yi
authors: EY
authors: Moon
authors: Young
authors: Y
authors: Yang
authors: Myeong-Sok
authors: MS
authors: Lee
authors: Jong-Seok
authors: JS
authors: Lim
mesh_terms: D000818:Animals; D002460:Cell Line; D004343:Drug Implants; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D008546:Melanoma, Experimental / Q000098:blood supply / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000139:chemically induced* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010316:Particle Size; D011205:Povidone / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012834:Silver / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D040521:Subcutaneous Tissue / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013499:Surface Properties; D042442:Vascular Endothelial Growth Factors / Q000378:metabolism
chemical_list: D004343:Drug Implants; D006454:Hemoglobins; D017382:Reactive Oxygen Species; D042442:Vascular Endothelial Growth Factors; D009569:Nitric Oxide; D012834:Silver; D040262:Receptors, Vascular Endothelial Growth Factor; D011205:Povidone