title: Preparation of amifostine polylactide-co-glycolide microspheres and its irradiation protective to mouse through oral administration.
abstract: The objectives of this study were to prepare the amifostine polylactide-co-glycolide (PLGA) microsphere and investigate its irradiation protective to mouse through oral administration. Amifostine-loaded PLGA microsphere was formulated using a modified double emulsion-solvent evaporation technique. The microsphere particle was spherical with a mean diameter of 2.8 ± 0.1 μm. Release data of amifostine PLGA microsphere was tested in phosphate-buffered saline at 37°C using a dialysis method and its release profiles was biphasic, showing a relatively large burst effect (50%) over the first 6 h, followed by a slower release phase, which sustained with 80% amifostine released in 48 h and almost 100% release till 6 days (144 h). A diffusion-controlled release model (Higuchi equation, R² = 0.9725) was obtained for amifostine releasing from PLGA microsphere. The radiation experiment was performed by applied cobalt-60 γ-radiation source. One hour before γ-radiation exposure, the mouse was orally given free amifostine and PLGA microsphere, respectively. The irradiation effects, such as blood cell concentration, superoxidase dismutase (SOD) activity and malondialdehyde (MDA) level were monitored. The results indicated that amifostine PLGA microsphere was more irradiation protective to mouse than that of free amifostine under the same oral administration route.
authors: Key Laboratory for Space Biosciences & Biotechnology, Faculty of Life Science, Northwestern Polytechnical University, Xi'an, China. lutinglixinxin@nwpu.edu.cn
authors: Ting-Li
authors: TL
authors: Lu
authors: Wei-guang
authors: WG
authors: Sun
authors: Wen
authors: W
authors: Zhao
authors: Tao
authors: T
authors: Chen
mesh_terms: D000284:Administration, Oral; D004999:Amifostine / Q000008:administration & dosage* / Q000737:chemistry; D000704:Analysis of Variance; D000818:Animals; D004337:Drug Carriers; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D008297:Male; D051379:Mice; D008863:Microspheres; D023421:Models, Animal; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011837:Radiation-Protective Agents / Q000008:administration & dosage* / Q000737:chemistry; D011897:Random Allocation
chemical_list: D004337:Drug Carriers; D011837:Radiation-Protective Agents; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004999:Amifostine