title: Correlation between eicosanoids in bronchoalveolar lavage fluid and in exhaled breath condensate.
abstract: Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation. In this study we have compared the concentrations of lipid mediators in EBC with concentrations in bronchoalveolar lavage fluid (BALF). We included 37 patients undergoing bronchoscopy (12 sarcoidosis, 12 COPD, 6 lung cancer, 5 chronic cough, 1 Wegener's granulomatosis, 1 sclerodermia). Patients were not allowed to have exacerbation or any change in concomitant medication for at least 4 weeks prior to the study. In all patients, EBC was collected immediately prior to the bronchoscopy. The levels of cys-LTs, LTB<formula>_{4}</formula>, 8-isoprostane were significantly higher in BALF compared to EBC (p < 0.0001, p< 0.001, p< 0.0001 for cys-LTs, LTB4, 8-isoprostane respectively). Moreover, there was a strong positive correlation between both leukotriene B<formula>_{4}</formula> and 8-isoprostane in BALF and EBC (r=0.53 and r=0.79, p< 0.01, respectively) in patients with sarcoidosis and COPD but there was no correlation between eicosanoids BALF and EBC in patients with chronic cough and lung cancer. This is the first study to compare EBC and BALF in different lung diseases which demonstrated significant correlations between the levels of eicosanoids in BALF and EBC in patients with COPD and sarcoidosis. EBC may be useful in measuring inflammation in several inflammatory lung diseases.
authors: Department of Pneumology & Allergology, Medical University of Lodz, Poland.
authors: Adam
authors: A
authors: Antczak
authors: Wojciech
authors: W
authors: Piotrowski
authors: Jerzy
authors: J
authors: Marczak
authors: Maciej
authors: M
authors: Ciebiada
authors: Pawel
authors: P
authors: Gorski
authors: Peter J
authors: PJ
authors: Barnes
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers; D001772:Blood Cell Count; D001944:Breath Tests / Q000379:methods*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D001999:Bronchoscopy / Q000379:methods; D016022:Case-Control Studies; D003545:Cysteine / Q000032:analysis*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D015232:Dinoprostone / Q000032:analysis*; D005260:Female; D006801:Humans; D015289:Leukotrienes / Q000032:analysis*; D008171:Lung Diseases / Q000097:blood / Q000175:diagnosis; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D015289:Leukotrienes; C112381:cysteinyl-leukotriene; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D015232:Dinoprostone; D003545:Cysteine