title: Simultaneous determination of histamine and prostaglandin D2 using an LC-ESI-MS/MS method with positive/negative ion-switching ionization modes: application to the study of anti-allergic flavonoids on the degranulation of KU812 cells.
abstract: A new method for simultaneous determination of histamine and prostaglandin D(2) (PGD(2)) by liquid chromatography-electrospray ionization tandem mass spectrometry operated in positive and negative ionization switching modes was developed and validated without a previous derivatization step. This method was used to measure histamine and PGD(2) release following degranulation of KU812 human basophilic cells, using pyrazol and d(4)-PGD(2) as internal standards. Analyses were performed on a liquid chromatography system employing a Cosmosil 5C(18) PAQ column and an isocratic elution with mixed solution of methanol-water (7:3, v/v) with 0.0015% trifluoroacetic acid at a flow rate of 0.2 mL/min. A triple-quadrupole mass spectrometer operating in selected reaction monitoring mode simultaneously monitored using the following transitions: positive m/z 112/95 for histamine and negative m/z 351/271 for PGD(2). The retention times of histamine and pyrazol were 4.2 and 5.0 min, respectively. PGD(2) and d(4)-PGD(2) had retention times of 8.5 min. The limits of detection were 0.3 and 0.5 ng/mL for histamine and PGD(2), respectively. The relative standard deviations of the retention time and peak area for histamine were between 1.6% and 7.7%, and were 1.2% and 7.8% for PGD(2). This method was used to evaluate the anti-allergic effects of 26 flavonoids and sodium cromoglicate which are first-line anti-allergic drugs. Of these compounds, baicalein and morin were the most potent inhibitors.
authors: Kobe Pharmaceutical University, Higashinada, Kobe, Japan. j-koyama@kobepharma-u.ac.jp
authors: Junko
authors: J
authors: Koyama
authors: Shiori
authors: S
authors: Taga
authors: Kae
authors: K
authors: Shimizu
authors: Maki
authors: M
authors: Shimizu
authors: Izumi
authors: I
authors: Morita
authors: Atsuko
authors: A
authors: Takeuchi
mesh_terms: D018926:Anti-Allergic Agents / Q000032:analysis*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis*; D006632:Histamine / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D015394:Molecular Structure; D015230:Prostaglandin D2 / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D013997:Time Factors
chemical_list: D018926:Anti-Allergic Agents; D005419:Flavonoids; D006632:Histamine; D015230:Prostaglandin D2