title: Artificial polymeric receptors on the cell surface promote the efficient cellular uptake of quantum dots.
abstract: In our previous paper, secondary-amine appended cationic polymer 1 was used as a scaffold to display artificial receptors on a cell surface (R. Kamitani et al., ChemBioChem, 2009, 10, 230). This polymer can be retained on the cell surface for more than 30 min before being slowly internalized into the cells. In this study, our aim is to achieve the efficient internalization of quantum dots (QDs) into target cells via artificial receptors on the polymer. As a receptor molecule, N-acetylglucosamine (GlcNAc) moieties were introduced into the polymer, and GlcNAc binding protein-displaying QDs were used as a ligand. We found that ligand-presenting QDs could be internalized effectively into cells via polymer-mediated endocytosis, whereas QDs were not internalized into untreated cells. These data suggest that our method based on cell-surface engineering using polymers affords a new approach to the delivery of various poorly permeable nanoparticles into cells.
authors: Research Institute for Electronic Science (RIES), Hokkaido University, Kita-Ku, Sapporo, Japan. kniikura@poly.es.hokudai.ac.jp
authors: Kenichi
authors: K
authors: Niikura
authors: Katsuyuki
authors: K
authors: Nambara
authors: Takaharu
authors: T
authors: Okajima
authors: Ryosuke
authors: R
authors: Kamitani
authors: Shin
authors: S
authors: Aoki
authors: Yasutaka
authors: Y
authors: Matsuo
authors: Kuniharu
authors: K
authors: Ijiro
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D004705:Endocytosis; D006801:Humans; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D045663:Quantum Dots; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D011108:Polymers; D014909:Wheat Germ Agglutinins; D000117:Acetylglucosamine