title: Coccolithophores: functional biodiversity, enzymes and bioprospecting.
abstract: Emiliania huxleyi is a single celled, marine phytoplankton with global distribution. As a key species for global biogeochemical cycling, a variety of strains have been amassed in various culture collections. Using a library consisting of 52 strains of E. huxleyi and an 'in house' enzyme screening program, we have assessed the functional biodiversity within this species of fundamental importance to global biogeochemical cycling, whilst at the same time determining their potential for exploitation in biocatalytic applications. Here, we describe the screening of E. huxleyi strains, as well as a coccolithovirus infected strain, for commercially relevant biocatalytic enzymes such as acid/alkali phosphodiesterase, acid/alkali phosphomonoesterase, EC1.1.1-type dehydrogenase, EC1.3.1-type dehydrogenase and carboxylesterase.
authors: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.|College of Life and Environmental Science, Biosciences, University of Exeter, Exeter, EX4 4QD, UK.
authors: Emma L
authors: EL
authors: Reid
authors: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.|Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK.
authors: Charlotte A
authors: CA
authors: Worthy
authors: CNRS-UPMC Station Biologique de Roscoff, Place Georges Teissier, 29682 Roscoff Cedex, France.
authors: Ian
authors: I
authors: Probert
authors: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.
authors: Sohail T
authors: ST
authors: Ali
authors: College of Life and Environmental Science, Biosciences, University of Exeter, Exeter, EX4 4QD, UK.
authors: John
authors: J
authors: Love
authors: Department of Biological Chemistry, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK.
authors: Johnathan
authors: J
authors: Napier
authors: College of Life and Environmental Science, Biosciences, University of Exeter, Exeter, EX4 4QD, UK.
authors: Jenny A
authors: JA
authors: Littlechild
authors: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.
authors: Paul J
authors: PJ
authors: Somerfield
authors: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.
authors: Michael J
authors: 0000-0001-8504-7171
authors: MJ
authors: Allen
mesh_terms: D055162:Biocatalysis; D044822:Biodiversity; D058087:Haptophyta / Q000737:chemistry / Q000201:enzymology*; D010839:Phytoplankton / Q000737:chemistry / Q000201:enzymology*