title: SUMOylation of Blimp-1 promotes its proteasomal degradation.
abstract: B lymphocyte induced maturation protein-1 (Blimp-1) is a transcription repressor of the Krueppel-like family. Blimp-1 plays important roles in developmental processes, such as of germ cells and hair follicle stem cells. In B lymphocytes Blimp-1 orchestrates the terminal differentiation into plasma cells. We discovered that Blimp-1 undergoes SUMOylation by SUMO-1. This SUMOylation is modulated by the SUMO protease SENP1. While Blimp-1 is relatively stable in 293T cells, a fusion with SUMO1 rendered it to rapid proteasomal degradation. Increase in SENP1 activity stabilized Blimp-1, while a decrease promoted its degradation. Our data indicate that SUMOylation of Blimp-1 regulates its intracellular stability.
authors: The Institute for Drug Research, The School of Pharmacy, The Hebrew University, Jerusalem, Israel.
authors: Livnat
authors: L
authors: Shimshon
authors: Avital
authors: A
authors: Michaeli
authors: Rivka
authors: R
authors: Hadar
authors: Stephen L
authors: SL
authors: Nutt
authors: Yael
authors: Y
authors: David
authors: Ami
authors: A
authors: Navon
authors: Ari
authors: A
authors: Waisman
authors: Boaz
authors: B
authors: Tirosh
mesh_terms: D002460:Cell Line; D003546:Cysteine Endopeptidases; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D000074462:Positive Regulatory Domain I-Binding Factor 1; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D055550:Protein Stability; D012097:Repressor Proteins / Q000378:metabolism*; D025842:SUMO-1 Protein / Q000378:metabolism; D058207:Sumoylation / Q000502:physiology*
chemical_list: D012097:Repressor Proteins; D025842:SUMO-1 Protein; C087042:PRDM1 protein, human; D000074462:Positive Regulatory Domain I-Binding Factor 1; D010450:Endopeptidases; C404512:SENP1 protein, human; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex