title: Laser sintering fabrication of three-dimensional tissue engineering scaffolds with a flow channel network.
abstract: The fabrication of tissue engineering scaffolds for the reconstruction of highly oxygen-dependent inner organs is discussed. An additive manufacturing technology known as selective laser sintering was employed to fabricate a highly porous scaffold with an embedded flow channel network. A porogen leaching system was used to obtain high porosity. A prototype was developed using the biodegradable plastic polycaprolactone and sodium chloride as the porogen. A high porosity of 90% was successfully obtained. Micro x-ray CT observation was carried out to confirm that channels with a diameter of approximately 1 mm were generated without clogging. The amount of residual salt was 930 µg while the overall volume of the scaffold was 13 cm(3), and it was confirmed that the toxicity of the salt was negligible. The hydrophilization of the scaffold to improve cell adhesion on the scaffold is also discussed. Oxygen plasma ashing and hydrolysis with sodium hydroxide, typically employed to improve the hydrophilicity of plastic surfaces, were tested. The improvement of hydrophilicity was confirmed by an increase in water retention by the porous scaffold from 180% to 500%.
authors: Institute of Industrial Science, University of Tokyo, Komaba 4-6-1, Meguro Tokyo, 153-8505, Japan. niino@iis.u-tokyo.ac.jp
authors: T
authors: T
authors: Niino
authors: D
authors: D
authors: Hamajima
authors: K
authors: K
authors: Montagne
authors: S
authors: S
authors: Oizumi
authors: H
authors: H
authors: Naruke
authors: H
authors: H
authors: Huang
authors: Y
authors: Y
authors: Sakai
authors: H
authors: H
authors: Kinoshita
authors: T
authors: T
authors: Fujii
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018920:Coculture Techniques / Q000379:methods; D005947:Glucose / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007834:Lasers; D051379:Mice; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D012709:Serum Albumin / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry / Q000494:pharmacology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; D012709:Serum Albumin; C016240:polycaprolactone; D012965:Sodium Chloride; D005947:Glucose