title: Negative electron transfer dissociation Fourier transform mass spectrometry of glycosaminoglycan carbohydrates.
abstract: Electron transfer through gas phase ion-ion reactions has led to the widespread application of electron- based techniques once only capable in ion trapping mass spectrometers. Although any mass analyzer can in theory be coupled to an ion-ion reaction device (typically a 3-D ion trap), some systems of interest exceed the capabilities of most mass spectrometers. This case is particularly true in the structural characterization of glycosaminoglycan (GAG) oligosaccharides. To adequately characterize highly sulfated GAGs or oligosaccharides above the tetrasaccharide level, a high resolution mass analyzer is required. To extend previous efforts on an ion trap mass spectrometer, negative electron transfer dissociation coupled with a Fourier transform ion cyclotron resonance mass spectrometer has been applied to increasingly sulfated heparan sulfate and heparin tetrasaccharides as well as a dermatan sulfate octasaccharide. Results similar to those obtained by electron detachment dissociation are observed.
authors: Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
authors: Franklin E
authors: FE
authors: Leach
authors: Jeremy J
authors: JJ
authors: Wolff
authors: Zhongping
authors: Z
authors: Xiao
authors: Melissa
authors: M
authors: Ly
authors: Tatiana N
authors: TN
authors: Laremore
authors: Sailaja
authors: S
authors: Arungundram
authors: Kanar
authors: K
authors: Al-Mafraji
authors: Andre
authors: A
authors: Venot
authors: Geert-Jan
authors: GJ
authors: Boons
authors: Robert J
authors: RJ
authors: Linhardt
authors: I Jonathan
authors: IJ
authors: Amster
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D003871:Dermatan Sulfate / Q000737:chemistry; D005583:Fourier Analysis; D006025:Glycosaminoglycans / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine
chemical_list: D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate