title: Escherichia coli translocation in experimental short bowel syndrome: probiotic supplementation and detection by polymerase chain reaction.
abstract: BACKGROUND
  After massive bowel resection, bacterial overgrowth is frequent and favors the occurrence of Gram-negative intestinal bacterial translocation (BT). Probiotics have been recommended in several diseases and may also have beneficial effects on BT. Conversely, polymerase chain reaction (PCR) technique has shown better sensitivity than conventional methods in bacterial detection and has not been investigated in experimental models of short bowel syndrome and BT.
  
  
  OBJECTIVE
  To test the hypothesis that Bifidobacterium lactis (BL) administration decreases Escherichia coli bacterial translocation (ECBT) in experimental short bowel syndrome and to confirm the better sensitivity of PCR technique to detect ECBT.
  
  
  METHODS
  Forty-eight adult Wistar rats, orally fed with standard rat chow and tap water ad libitum, were maintained in individual metabolic cages for 10 days and divided into three groups:                                                                 Control group (n = 15): non-manipulated animals.                                                                                                RES group (n = 15): 80% gut resection. 1 ml of sterile water was administered daily after orogastric intubation.                                                                                                RES-PRO group (n = 18): same resection as RES group and daily administration of 7.8 × 10(9) BL (CFU).                                                                                                             At the end of the study, portal blood, peripheral blood and mesenteric lymph node (MLN) samples were recovered and cultured. Also, genomic DNA from E. coli was detected by PCR technique.
  
  
  RESULTS
  In conventional culture there was no ECBT in control animals whereas 73% of RES and 33% of RES-PRO animals showed it. PCR detected ECBT in 47, 87 and 33%, respectively, showing higher sensitivity.
authors: Donostia Hospital, Osakidetza, San Sebastián, Spain. ignacio.eizaguirre@osakidetza.net
authors: Inaki
authors: I
authors: Eizaguirre
authors: Pablo
authors: P
authors: Aldazabal
authors: Nerea Garcia
authors: NG
authors: Urkia
authors: Anabel
authors: A
authors: Asensio
authors: Jose María García
authors: JM
authors: Arenzxana
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D001644:Bifidobacterium; D004269:DNA, Bacterial / Q000032:analysis*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology*; D007421:Intestine, Small / Q000382:microbiology / Q000601:surgery; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000188:drug therapy / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial