title: Expression of thyroid-specific transcription factors in thyroid carcinoma, contralateral thyroid lobe and healthy thyroid gland in dogs.
abstract: Thyrotropin receptor (TSH-R), thyroglobulin (Tg), thyroperoxidase (TPO), thyroid specific transcription factor-1 (TTF-1), paired box 8 transcription factor (PAX-8), insulin like growth factor-1 (IGF-1) and estrogen receptor alpha (ERα) transcripts were determined by real-time PCR in follicular carcinoma and contralateral (CL) lobes, and healthy thyroid canine glands. Concentrations of TSH-R, PAX-8, and ERα mRNA were not different among groups; the carcinoma group had lower Tg and TPO mRNA than healthy and CL groups, while no differences were found between the two latter groups, suggesting that the carcinoma tissue presents an altered capacity to synthesize thyroid hormones. The transcription factor that promotes thyrocytes proliferation, TTF-1 as well as IGF-1, presented a greater mRNA expression in the CL group, suggesting that the CL lobe may function in a compensatory state.
authors: Laboratory of Nuclear Techniques, Veterinary Faculty, University of Uruguay, Lasplaces 1550, 11600 Montevideo, Uruguay. ppessina@hotmail.com
authors: P
authors: P
authors: Pessina
authors: V
authors: V
authors: Castillo
authors: M
authors: M
authors: Araújo
authors: M
authors: M
authors: Carriquiry
authors: A
authors: A
authors: Meikle
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D047628:Estrogen Receptor alpha / Q000096:biosynthesis*; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D007453:Iodide Peroxidase / Q000096:biosynthesis*; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis*; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011989:Receptors, Thyrotropin / Q000096:biosynthesis*; D013954:Thyroglobulin / Q000096:biosynthesis*; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000378:metabolism*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D047628:Estrogen Receptor alpha; D009687:Nuclear Proteins; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499495:Pax8 protein, Canis familiaris; D011989:Receptors, Thyrotropin; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D007334:Insulin-Like Growth Factor I; D013954:Thyroglobulin; D007453:Iodide Peroxidase