title: Sonic hedgehog agonist fails to induce neural stem cell precursors in a porcine model of experimental intracranial hemorrhage.
abstract: BACKGROUND
  The role of endogenous neural stem cell progenitors in recovery from intracranial hemorrhage remains to be elucidated. Proliferation of such stem cells in the subventricular zone has been described in rodent models of experimental intracranial hemorrhage. Administration of a sonic hedgehog agonist at the time of hemorrhage was hypothesized to increase the quantity of such precursor cells.
  
  
  METHODS
  Two groups of pigs were subjected to injection of autologous blood into the right frontal lobe. One group was also injected at the same site with a sonic hedgehog agonist at the time of the hemorrhage to stimulate cell growth, and the other was given a vehicle control. The pigs received intravenous BrdU for 5 days postoperatively to label replicating cells, and then were sacrificed at intervals up to 21 days.
  
  
  RESULTS
  Pigs in the hemorrhage only group demonstrated increased and more persistent BrdU staining in the subventricular zone relative to pigs in the group that received sonic hedgehog agonist. The latter group demonstrated increased BrdU activity in non-neural lineage cells in the area of the hemorrhage.
  
  
  CONCLUSIONS
  Sonic hedgehog agonist did not induce subventricular zone neural stem cell progenitor division after experimental intracranial hemorrhage in a pig model.
authors: Department of Neurosurgery, University of Rochester, New York, NY 10010, USA.
authors: Jing
authors: J
authors: Tong
authors: Jonathan M
authors: JM
authors: Latzman
authors: Judah
authors: J
authors: Rauch
authors: David S
authors: DS
authors: Zagzag
authors: Jason H
authors: JH
authors: Huang
authors: Uzma
authors: U
authors: Samadani
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002552:Cerebral Ventricles / Q000473:pathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000089622:Doublecortin Domain Proteins; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D053823:Hedgehog Proteins / Q000819:agonists*; D020300:Intracranial Hemorrhages / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects*; D009479:Neuropeptides / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D013552:Swine; D013997:Time Factors
chemical_list: D000089622:Doublecortin Domain Proteins; D005904:Glial Fibrillary Acidic Protein; D053823:Hedgehog Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D011725:Pyridines; C546888:Shh protein, rat; D010751:Phosphopyruvate Hydratase; D001973:Bromodeoxyuridine