title: [Expression of annexin A7 in spermatogonial stem cells].
abstract: OBJECTIVE
  To study the expression of Annexin A7 in the mouse testis, especially in different types of spermatogonia.
  
  
  METHODS
  We prepared Annexin A7 recombinant protein using prokaryotic expression, adsorbed the Annexin A7 antibody with it after identified by mass spectrometry, and detected the expression of Annexin A7 by Western-blot and immunohistochemistry.
  
  
  RESULTS
  Annexin A7 was expressed in a development-dependent manner in the spermatogonia of the prepubertal mice and in the type-A single (As) and type-A paired (Apr) spermatogonia of adult mice. These results were confirmed by the co-localization of Annexin A7 and Stra8, a known determinant of differentiated spermatogonial stem cells (SSCs).
  
  
  CONCLUSIONS
  Annexin A7 is the internal factor of As and Apr spermatogonia, which might be involved in the biological functions of SSCs.
authors: Jiangsu Provincial Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China.
authors: Chun-Mei
authors: CM
authors: Yu
authors: Ping
authors: P
authors: Zhang
authors: Jing
authors: J
authors: Wang
authors: Ming-Xi
authors: MX
authors: Liu
authors: Hui
authors: H
authors: Wang
authors: Zuo-Min
authors: ZM
authors: Zhou
authors: Jia-Hao
authors: JH
authors: Sha
mesh_terms: D000818:Animals; D017310:Annexin A7 / Q000378:metabolism*; D008297:Male; D051379:Mice; D013093:Spermatogonia / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D017310:Annexin A7