title: Biodegradation kinetics of 4-fluorocinnamic acid by a consortium of Arthrobacter and Ralstonia strains.
abstract: Arthrobacter sp. strain G1 is able to grow on 4-fluorocinnamic acid (4-FCA) as sole carbon source. The organism converts 4-FCA into 4-fluorobenzoic acid (4-FBA) and utilizes the two-carbon side-chain for growth with some formation of 4-fluoroacetophenone as a dead-end side product. We also have isolated Ralstonia sp. strain H1, an organism that degrades 4-FBA. A consortium of strains G1 and H1 degraded 4-FCA with Monod kinetics during growth in batch and continuous cultures. Specific growth rates of strain G1 and specific degradation rates of 4-FCA were observed to follow substrate inhibition kinetics, which could be modeled using the kinetic models of Haldane-Andrew and Luong-Levenspiel. The mixed culture showed complete mineralization of 4-FCA with quantitative release of fluoride, both in batch and continuous cultures. Steady-state chemostat cultures that were exposed to shock loadings of substrate responded with rapid degradation and returned to steady-state in 10-15 h, indicating that the mixed culture provided a robust system for continuous 4-FCA degradation.
authors: Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.
authors: Syed A
authors: SA
authors: Hasan
authors: Piet
authors: P
authors: Wietzes
authors: Dick B
authors: DB
authors: Janssen
mesh_terms: D001173:Arthrobacter / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001565:Benzoates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D002934:Cinnamates / Q000378:metabolism*; D007700:Kinetics; D059013:Microbial Consortia; D043367:Ralstonia / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism
chemical_list: D001565:Benzoates; D002934:Cinnamates; D014874:Water Pollutants, Chemical; C042046:4-fluorocinnamic acid; D002244:Carbon; C022893:4-fluorobenzoic acid