title: Accuracy of differential diagnosis for pancreatic cancer is improved in the combination of RCAS1 and CEA measurements and cytology in pancreatic juice.
abstract: Improvement of diagnostic accuracy for pancreatic cancer in pancreatic disease patients was investigated by examining the combination of three diagnostic methods, i.e., measurements of RCAS1 and CEA levels in pancreatic juice and pancreatic juice cytology. Pancreatic juice was collected from 12 pancreatic cancer (PC) and 26 non-PC patients. RCAS1 and CEA levels were measured by using ELISA. RCAS1 expression on surgically resected tissue was immunohistochemically examined for 2 PC patients. By setting the cutoff level of RCAS1 at 10 U/ml and that of CEA at 18.5 μg/ml, sensitivity of RCAS1 was 42% and that of CEA was 50%. On the other hand, sensitivity and specificity increased from 42% and 85% of RCAS1 alone to 75% and 85% in the examination of RCAS1 + CEA + cytology, and the false-negative rate was also reduced to 25% in this combination. Immunohistochemically, a patient with a high RCAS1 level in pancreatic juice had numerous RCAS1-positive tumor cells in the pancreatic juice. We concluded that RCAS1 and CEA measurements together with cytology in pancreatic juice would be a useful combination method for making a differential diagnosis of PC from non-PC.
authors: Department of Pathology, Kurume University School of Medicine, 67 Asahimachi, Kurume, Fukuoka, 830-0011, Japan. nyoshiki@med.kurume-u.ac.jp
authors: Yoshiki
authors: Y
authors: Naito
authors: Yoshinobu
authors: Y
authors: Okabe
authors: Masakatsu
authors: M
authors: Nagayama
authors: Takuya
authors: T
authors: Nishinakagawa
authors: Tomoki
authors: T
authors: Taira
authors: Akihiko
authors: A
authors: Kawahara
authors: Satoshi
authors: S
authors: Hattori
authors: Kazuyuki
authors: K
authors: Machida
authors: Yusuke
authors: Y
authors: Ishida
authors: Ryohei
authors: R
authors: Kaji
authors: Kazuhiro
authors: K
authors: Mikagi
authors: Hisafumi
authors: H
authors: Kinoshita
authors: Makiko
authors: M
authors: Yasumoto
authors: Jun
authors: J
authors: Akiba
authors: Masayoshi
authors: M
authors: Kage
authors: Manabu
authors: M
authors: Nakashima
authors: Koichi
authors: K
authors: Ohshima
authors: Hirohisa
authors: H
authors: Yano
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000276:immunology; D003581:Cytodiagnosis; D003584:Cytological Techniques; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010182:Pancreatic Diseases / Q000175:diagnosis / Q000276:immunology; D010189:Pancreatic Juice / Q000166:cytology / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000175:diagnosis / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; C120762:EBAG9 protein, human