title: Cu2+ triggers reversible aggregation of a disordered His-rich dehydrin MpDhn12 from Musa paradisiaca.
abstract: Copper is an essential nutrient, but it is toxic in excess. Here, we cloned and characterized a His-rich low molecular weight dehydrin from Musa paradisiaca, MpDhn12. Analysis by circular dichroism (CD) spectra and a thermal stability assay showed that MpDhn12 is an intrinsically disordered protein, and immobilized-metal affinity chromatography (IMAC) analysis revealed that MpDhn12 can bind Cu(2+) both in vitro and in vivo. Interestingly, MpDhn12 aggregated under excess Cu(2+) conditions, and the aggregation was reversible and impaired by histidine modification with diethylpyrocarbonate (DEPC), while the disordered structure of another dehydrin ERD14 (as a control) was not changed. Furthermore, MpDhn12 could complement the copper-sensitive phenotype of yeast mutant Δsod1. These results together suggested that MpDhn12 may take part in buffering copper levels through chelation and formation of aggregates in excess Cu(2+) conditions. To the best of our knowledge, it is the first report that a dehydrin interchanged between disordered and aggregated state triggered by copper.
authors: State Key Laboratory for Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou, P. R. China.
authors: Peiqiang
authors: P
authors: Mu
authors: Dongru
authors: D
authors: Feng
authors: Jianbin
authors: J
authors: Su
authors: Yang
authors: Y
authors: Zhang
authors: Jinran
authors: J
authors: Dai
authors: Honglei
authors: H
authors: Jin
authors: Bing
authors: B
authors: Liu
authors: Yanming
authors: Y
authors: He
authors: Kangbiao
authors: K
authors: Qi
authors: Hongbin
authors: H
authors: Wang
authors: Jinfa
authors: J
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D003300:Copper / Q000494:pharmacology*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D028521:Musa / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment
chemical_list: D010940:Plant Proteins; C070556:dehydrin proteins, plant; D003300:Copper