title: Fluorescence recovery after photobleaching.
abstract: This chapter describes the use of microscope-based fluorescence recovery after photobleaching (FRAP). To quantify the dynamics of proteins within a subcellular compartment, we first outline the general aspects of FRAP experiments and then provide a detailed protocol of how to measure and analyse the most important parameters of FRAP experiments such as mobile fraction and half-time of recovery.
authors: Faculty of Life Sciences, The University of Manchester, Manchester, UK.
authors: Alex
authors: A
authors: Carisey
authors: Matthew
authors: M
authors: Stroud
authors: Ricky
authors: R
authors: Tsang
authors: Christoph
authors: C
authors: Ballestrem
mesh_terms: D000465:Algorithms; D000818:Animals; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques; D036681:Fluorescence Recovery After Photobleaching / Q000295:instrumentation / Q000379:methods*; D022001:Focal Adhesions / Q000737:chemistry / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012984:Software; D059008:Time-Lapse Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D015815:Cell Adhesion Molecules; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins