title: First evidence of palytoxin and 42-hydroxy-palytoxin in the marine cyanobacterium Trichodesmium.
abstract: Marine pelagic diazotrophic cyanobacteria of the genus Trichodesmium (Oscillatoriales) are widespread throughout the tropics and subtropics, and are particularly common in the waters of New Caledonia. Blooms of Trichodesmium are suspected to be a potential source of toxins in the ciguatera food chain and were previously reported to contain several types of paralyzing toxins. The toxicity of water-soluble extracts of Trichodesmium spp. were analyzed by mouse bioassay and Neuroblastoma assay and their toxic compounds characterized using liquid chromatography coupled with tandem mass spectrometry techniques. Here, we report the first identification of palytoxin and one of its derivatives, 42-hydroxy-palytoxin, in field samples of Trichodesmium collected in the New Caledonian lagoon. The possible role played by Trichodesmium blooms in the development of clupeotoxism, this human intoxication following the ingestion of plankton-eating fish and classically associated with Ostreopsis blooms, is also discussed.
authors: Toulouse University, UPS, UMR152 UPS-IRD (PHARMA-DEV), 118, route de Narbonne, F-31062 Toulouse cedex 9, France.|Research Institute for the Development (IRD), UMR152, 98848 Noumea, New Caledonia.
authors: Anne Sophie
authors: AS
authors: Kerbrat
authors: Laboratory of Phycotoxins, IFREMER, Rue de l'Ile d'Yeu, BP21105, F-44311 Nantes cedex 3, France.
authors: Zouher
authors: 0000-0001-8078-9459
authors: Z
authors: Amzil
authors: Laboratory of toxic micro-algae (LMT), Louis Malarde Institute (ILM), BP30, 98713 Papeete, Tahiti, French Polynesia.
authors: Ralph
authors: R
authors: Pawlowiez
authors: Biological Science Center, Boston University, 5 Cummington Street, Boston, MA 02215, USA.
authors: Stjepko
authors: S
authors: Golubic
authors: Laboratory of Phycotoxins, IFREMER, Rue de l'Ile d'Yeu, BP21105, F-44311 Nantes cedex 3, France.
authors: Manoella
authors: 0000-0001-5105-6467
authors: M
authors: Sibat
authors: Laboratory of toxic micro-algae (LMT), Louis Malarde Institute (ILM), BP30, 98713 Papeete, Tahiti, French Polynesia.
authors: Helene Taiana
authors: 0000-0002-1184-7034
authors: HT
authors: Darius
authors: Laboratory of toxic micro-algae (LMT), Louis Malarde Institute (ILM), BP30, 98713 Papeete, Tahiti, French Polynesia.
authors: Mireille
authors: M
authors: Chinain
authors: Toulouse University, UPS, UMR152 UPS-IRD (PHARMA-DEV), 118, route de Narbonne, F-31062 Toulouse cedex 9, France.|Research Institute for the Development (IRD), UMR152, 98713 Papeete, Tahiti, French Polynesia.
authors: Dominique
authors: D
authors: Laurent
mesh_terms: D000178:Acrylamides / Q000302:isolation & purification / Q000633:toxicity*; D000818:Animals; D001681:Biological Assay / Q000379:methods; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003064:Cnidarian Venoms / Q000302:isolation & purification / Q000633:toxicity*; D000458:Cyanobacteria / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D011714:Pyrans / Q000302:isolation & purification / Q000633:toxicity*; D053719:Tandem Mass Spectrometry; D018675:Toxicity Tests; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D003064:Cnidarian Venoms; D011714:Pyrans; C547187:42-hydroxypalytoxin; D000095702:Polyether Toxins; C010272:palytoxin