title: Size separation of colloidally dispersed nanoparticles using a monolithic capillary column.
abstract: We developed a method to separate colloidally dispersed nanoparticles on monolithic capillary columns. Silica nanoparticles were eluted according to their sizes, and the plots of the logarithm of the size of nanoparticles against their elution volume showed good linearity (r=0.992) over wide range of sizes. Because of the high porosity of the monolithic column (porosity; 88%), the column's length could be increased without clogging of the dispersed samples and the pressure in a long column (500 mm × 0.2 mm i.d.) was low, with a value of 5.8 MPa at a flow rate of 1 μL/min. We demonstrate that this method using monolithic capillary columns could be used as a powerful tool for size separation of nanometer-size materials, which will open a new pathway to quality control of nanomaterials in nanotechnology applications.
authors: Division of Drugs, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. kumikato@nihs.go.jp
authors: Kumiko
authors: K
authors: Sakai-Kato
authors: Shigenori
authors: S
authors: Ota
authors: Toyohide
authors: T
authors: Takeuchi
authors: Toru
authors: T
authors: Kawanishi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D003102:Colloids / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D003102:Colloids; D012822:Silicon Dioxide