title: Real-time analysis of living animals by electrospray ionization mass spectrometry.
abstract: Probe electrospray ionization (PESI) is one of the most promising methods in biochemical analysis because it enables us to analyze biological samples very quickly without any special pretreatment. Moreover, due to the small size of the needle tip, this method has advantages such as low invasiveness to the samples, making it possible to analyze the biological profiles of organs or tissues in living animal in situ. In this study, we performed a real-time analysis of living mice that delineates the differences in lipid composition of hepatocytes between normal and steatotic mice. In steatotic mice, the number of peaks and the ion abundance for triacylglycerols were much higher compared with those of control mice. All mice used in this study tolerated the procedure well and survived for more than a month until sacrificed for further analysis. To test a potential for medical diagnosis, human tumor tissues were also measured and we obtained discriminative results judged as useful for diagnostics. These results pave the way into the application of PESI to the in vivo analysis of biological molecules.
authors: Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
authors: Kentaro
authors: K
authors: Yoshimura
authors: Lee Chuin
authors: LC
authors: Chen
authors: Zhan
authors: Z
authors: Yu
authors: Kenzo
authors: K
authors: Hiraoka
authors: Sen
authors: S
authors: Takeda
mesh_terms: D000818:Animals; D003199:Computer Systems; D005234:Fatty Liver / Q000175:diagnosis*; D022781:Hepatocytes / Q000737:chemistry*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis
chemical_list: D008055:Lipids; D014280:Triglycerides