title: Opsonization, biodistribution, cellular uptake and apoptosis study of PEGylated PBCA nanoparticle as potential drug delivery carrier.
abstract: OBJECTIVE
  For nanocarrier-based targeted delivery systems, preventing phagocytosis for prolong circulation half life is a crucial task. PEGylated poly(n-butylcyano acrylate) (PBCA) NP has proven a promising approach for drug delivery, but an easy and reliable method of PEGylation of PBCA has faced a major bottleneck.
  
  
  METHODS
  PEGylated PBCA NPs containing docetaxel (DTX) by modified anionic polymerization reaction in aqueous acidic media containing amine functional PEG were made as an single step PEGylation method. In vitro colloidal stability studies using salt aggregation method and antiopsonization property of prepared NPs using mouse macrophage cell line RAW264 were performed. In vitro performance of anticancer activity of prepared formulations was checked on MCF7 cell line. NPs were radiolabeled with 99mTc and intravenously administered to study blood clearance and biodistribution in mice model.
  
  
  RESULTS
  These formulations very effectively prevented phagocytosis and found excellent carrier for drug delivery purpose. In vivo studies display long circulation half life of PBCA-PEG20 NP in comparison to other formulations tested.
  
  
  CONCLUSIONS
  The PEGylated PBCA formulation can work as a novel tool for drug delivery which can prevent RES uptake and prolong circulation half life.
authors: Pharmacy Department TIFAC Centre of Relevance & Excellence in New Drug Delivery Systems G.H. Patel Pharmacy Building, The Maharaja Sayajirao University of Baroda, Donor's Plaza, Fatehgunj, Vadodara 390002, India.
authors: Kiran Ramanlal
authors: KR
authors: Chaudhari
authors: Mukesh
authors: M
authors: Ukawala
authors: Arehalli S
authors: AS
authors: Manjappa
authors: Abhinesh
authors: A
authors: Kumar
authors: Piyush Kishor
authors: PK
authors: Mundada
authors: Anil Kumar
authors: AK
authors: Mishra
authors: Rashi
authors: R
authors: Mathur
authors: Jukka
authors: J
authors: Mönkkönen
authors: Rayasa S Ramchandra
authors: RS
authors: Murthy
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics*; D004659:Enbucrilate / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D006207:Half-Life; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D053758:Nanoparticles; D010587:Phagocytosis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics*; D043823:Taxoids / Q000008:administration & dosage; D013667:Technetium / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D043823:Taxoids; D000077143:Docetaxel; D011092:Polyethylene Glycols; D013667:Technetium; D004659:Enbucrilate