title: Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system.
abstract: BACKGROUND
  The involvement of mitochondria in pathological states, such as neurodegenerative diseases, sepsis, stroke, and cancer, are well documented. Monitoring of nicotinamide adenine dinucleotide (NADH) fluorescence in vivo as an intracellular oxygen indicator was established in 1950 to 1970 by Britton Chance and collaborators. We use a multiparametric monitoring system enabling assessment of tissue vitality. In order to use this technology in clinical practice, the commercial developed device, the CritiView (CRV), is tested in animal models as well as in patients.
  
  
  RESULTS
  The new CRV enables the optical monitoring of four different parameters, representing the energy balance of various tissues in vivo. Mitochondrial NADH is measured by surface fluorometry/reflectometry. In addition, tissue microcirculatory blood flow, tissue reflectance and oxygenation are measured as well. The device is tested both in vitro and in vivo in a small animal model and in preliminary clinical trials in patients undergoing vascular or open heart surgery. In patients, the monitoring is started immediately after the insertion of a three-way Foley catheter (urine collection) to the patient and is stopped when the patient is discharged from the operating room. The results show that monitoring the urethral wall vitality provides information in correlation to the surgical procedure performed.
authors: Bar-Ilan University, The Mina & Everard Goodman Faculty of Life-Sciences, Ramat-Gan, 52900 Israel. mayevskya@gmail.com
authors: Avraham
authors: A
authors: Mayevsky
authors: Raphael
authors: R
authors: Walden
authors: Eliyahu
authors: E
authors: Pewzner
authors: Assaf
authors: A
authors: Deutsch
authors: Eitan
authors: E
authors: Heldenberg
authors: Jacob
authors: J
authors: Lavee
authors: Salis
authors: S
authors: Tager
authors: Erez
authors: E
authors: Kachel
authors: Ehud
authors: E
authors: Raanani
authors: Sergey
authors: S
authors: Preisman
authors: Violete
authors: V
authors: Glauber
authors: Eran
authors: E
authors: Segal
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002245:Carbon Dioxide / Q000378:metabolism; D006348:Cardiac Surgical Procedures; D003422:Critical Care; D004734:Energy Metabolism; D005470:Fluorometry; D005849:Gerbillinae; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D016014:Linear Models; D008833:Microcirculation; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology*; D008954:Models, Biological; D016343:Monitoring, Intraoperative / Q000295:instrumentation* / Q000379:methods*; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D019265:Spectroscopy, Near-Infrared; D014521:Urethra / Q000166:cytology / Q000502:physiology
chemical_list: D009243:NAD; D002245:Carbon Dioxide; D010100:Oxygen