title: Resistance to powdery mildew in Spanish barley landraces is controlled by different sets of quantitative trait loci.
abstract: Twenty-two landrace-derived inbred lines from the Spanish Barley Core Collection (SBCC) were found to display high levels of resistance to a panel of 27 isolates of the fungus Blumeria graminis that exhibit a wide variety of virulences. Among these lines, SBCC145 showed high overall resistance and a distinctive spectrum of resistance compared with the other lines. Against this background, the main goal of the present work was to investigate the genetic basis underlying such resistance using a doubled haploid population derived from a cross between SBCC145 and the elite spring cultivar Beatrix. The population was genotyped with the 1,536-SNP Illumina GoldenGate Oligonucleotide Pool Assay (Barley OPA-1 or BOPA1 for short), whereas phenotypic analysis was performed using two B. graminis isolates. A major quantitative trait locus (QTL) for resistance to both isolates was identified on the long arm of chromosome 6H (6HL) and accounted for ca. 60% of the phenotypic variance. Depending on the B. graminis isolate tested, three other minor QTLs were detected on chromosomes 2H and 7H, which explained less than 5% of the phenotypic variation each. In all cases, the alleles for resistance derived from the Spanish parent SBCC145. The position, the magnitude of the effect observed and the proportion of phenotypic variation accounted for by the QTL on 6HL suggest this is a newly identified locus for broad-based resistance to powdery mildew.
authors: Department of Genetics and Plant Production, Aula Dei Experimental Station, EEAD-CSIC, Avda Montañana 1005, 50059 Zaragoza, Spain.
authors: C
authors: C
authors: Silvar
authors: A M
authors: AM
authors: Casas
authors: E
authors: E
authors: Igartua
authors: L J
authors: LJ
authors: Ponce-Molina
authors: M P
authors: MP
authors: Gracia
authors: G
authors: G
authors: Schweizer
authors: M
authors: M
authors: Herz
authors: K
authors: K
authors: Flath
authors: R
authors: R
authors: Waugh
authors: D
authors: D
authors: Kopahnke
authors: F
authors: F
authors: Ordon
mesh_terms: D000483:Alleles; D001203:Ascomycota / Q000472:pathogenicity*; D001947:Breeding; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D007113:Immunity, Innate; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D040641:Quantitative Trait Loci
chemical_list: D005819:Genetic Markers