title: Photoactivated green fluorescence emission by femtosecond oscillator from indole solutions.
abstract: We reported a novel femtosecond-laser-activated fluorescence emission from indole solutions upon excitation by the second harmonic wavelength of a femtosecond oscillator. A new absorption band around 400 nm and corresponding fluorescent band in the green domain were produced after the irradiation of femtosecond laser. This femtosecond-laser-activated luminescence process that allows the use of visible wavelength as a substitute for UV light to excite fluorescence from indole would extend applications based on indole chromophore. Furthermore, the photoactived emission can act as a fluorescence lifetime probe to measure the polarity in complex biological systems since it is polarity-sensitive. High performance liquid chromatography with fluorescence detector (HPLC-FLU) and high performance liquid chromatography with mass spectrometer (HPLC-MS) analysis demonstrate that the origin of the photoactivated fluorescence is new molecular species that generated in indole solution upon femtosecond laser irradiation.
authors: State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China. wangchen@mail.siom.ac.cn
authors: Chen
authors: C
authors: Wang
authors: Lei
authors: L
authors: Feng
authors: Li
authors: L
authors: Liu
authors: Yuanlong
authors: Y
authors: Wang
authors: Lingling
authors: L
authors: Qiao
authors: Jielei
authors: J
authors: Ni
authors: Jian
authors: J
authors: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D007211:Indoles / Q000737:chemistry*; D007834:Lasers; D013058:Mass Spectrometry; D009991:Oscillometry; D055668:Photochemical Processes; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014466:Ultraviolet Rays
chemical_list: D007211:Indoles; D012996:Solutions; C030374:indole