title: Covalent heparin modification of a polysulfone flat sheet membrane for selective removal of low-density lipoproteins: a simple and versatile method.
abstract: A simple, convenient and economical method for the heparinization of PSf membranes is described, with the aim of preparing an LDL adsorber for simultaneous LDL apheresis and hemodialysis. An atmospheric pressure glow discharge generator is used to activate the PSf membrane surface, with subsequent chemical binding of heparin in the presence of EDC and NHS. ATR-FTIR spectroscopy and XPS measurements confirm successful surface modification. The PSf-Hep membrane shows good blood compatibility, with a relatively low amount and normal morphology of adherent platelets. ELISA results indicate that the PSf-Hep membrane exhibits excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in hemodialysis with simultaneous LDL removal.
authors: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors: Jing
authors: J
authors: Li
authors: Xiao-Jun
authors: XJ
authors: Huang
authors: Jing
authors: J
authors: Ji
authors: Ping
authors: P
authors: Lan
authors: Jörg
authors: J
authors: Vienken
authors: Thomas
authors: T
authors: Groth
authors: Zhi-Kang
authors: ZK
authors: Xu
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry*; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods; D001792:Blood Platelets / Q000378:metabolism; D006493:Heparin / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification*; D010973:Platelet Adhesiveness; D011108:Polymers / Q000737:chemistry*; D006435:Renal Dialysis / Q000295:instrumentation / Q000379:methods; D013450:Sulfones / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D008077:Lipoproteins, LDL; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; D006493:Heparin