title: [Construction and identification of BIRC5 shRNA lentiviral expression vector].
abstract: OBJECTIVE
  Design and synthesis complementary DNA sequences of 3 pairs of short hairpin structure and a pair of negative control sequence by RNA interference technique and construct and identify a lentiviral interference vector with human BIRC5 gene as target gene.
  
  
  METHODS
  The designed and synthesised Single-Stranded primer were annealed to Double-Stranded oligo sequences and subcloned into linear pMAGic lentiviral plasmid vector digested by enzyme Age I and EcoR I. Screening positive clone after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing.
  
  
  RESULTS
  335 bp straps of positive clone and 298 bp straps of negative clone form PCR amplification production have been obtained after gel electrophoresis, the designed and synthesised sequences have been contained in these clone straps confirmed by the result of DNA sequencing.
  
  
  CONCLUSIONS
  Four pairs of BIRC5 shRNA recombinant lentiviral expression vector were constructed successfully, which laid the foundation for researching the inhibition of BIRC5 siRNA target against tumor cells proliferation, induction apoptosis and gene therapy.
authors: Medicine College of Xi'an Jiaotong University, Xi'an, China.
authors: Guo-zhou
authors: GZ
authors: Rao
authors: Ang
authors: A
authors: Li
authors: Yong-jin
authors: YJ
authors: Zhu
authors: Jian-feng
authors: JF
authors: Shi
authors: Jian-zhong
authors: JZ
authors: Gou
authors: Yin-cheng
authors: YC
authors: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000172:deficiency* / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015202:Protein Engineering / Q000379:methods*; D034741:RNA, Small Interfering / Q000235:genetics*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin