title: Antimicrobial resistance of Staphylococcus pseudintermedius isolates from healthy dogs and dogs affected with pyoderma in Japan.
abstract: Staphylococcus pseudintermedius strains were isolated from healthy dogs and dogs with pyoderma in 2000-2002 and 2009. All the isolates from dogs with pyoderma in 1999-2000 and from healthy dogs in 2000-2002 and 2009 were susceptible to cefalexin and/or other cephalosporins and oxacillin. However, 7.1-12.5 and 11.4% of S. pseudintermedius isolates from dogs with pyoderma in 2009 were resistant to cephalosporins and oxacillin, respectively. All S. pseudintermedius isolates from dogs with pyoderma in 1999-2000 and those from healthy dogs in 2000-2002 were susceptible to fluoroquinolones; however, 50% of the S. pseudintermedius strains isolated from dogs with pyoderma in 2009 and 30% of the S. pseudintermedius strains isolated from healthy dogs in 2009 were resistant to fluoroquinolones. Of the 21 oxacillin-resistant S. pseudintermedius (MRSP) isolates, 11 carried SCCmec type V and 10 carried hybrid SCCmec types II-III. Staphylococcus pseudintermedius strains that were resistant to only one of three fluoroquinolones had a mutation in the quinolone resistance determination region of grlA, whereas S. pseudintermedius strains that were resistant to two or more fluoroquinolones had mutations in the quinolone resistance determination regions of both grlA and gyrA.
authors: Laboratory of Veterinary Microbiology, Faculty of Veterinary Medicine, Kitasato University, Aomori 034-8628, Japan.
authors: Kenta
authors: K
authors: Onuma
authors: Taishi
authors: T
authors: Tanabe
authors: Hisaaki
authors: H
authors: Sato
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use; D004283:Dog Diseases / Q000188:drug therapy / Q000382:microbiology*; D004285:Dogs / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D007564:Japan; D016106:Methicillin Resistance; D011711:Pyoderma / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000890:Anti-Infective Agents