title: [A sensitive analytical method for six aflatoxins in rainbow trout muscle and liver].
abstract: A highly sensitive analysis method for six aflatoxins (aflatoxin B₁, B₂, G₁, G₂, M₁ and aflatoxicol) in rainbow trout muscle and liver was developed. Aflatoxins (AFs) were extracted with acetonitrile-water (9 : 1), purified on an immunoaffinity column, and subjected to HPLC with fluorescence detection after post-column photochemical derivatization. The recoveries of AFs at 0.05 μg/kg spiking levels were 71.4-82.4% in muscle and 80.1-93.0% in liver, and the repeatability relative standard deviations (RSDr) were 0.87-4.6% in muscle and 2.0-6.2% in liver. Limits of quantitation (LOQs) and limits of detection(LODs)of AFs were estimated to be 0.004-0.029 μg/kg, and 0.002-0.012 μg/kg, respectively.
authors: Japan Food Research Laboratories, Tama Laboratory, Tokyo. ogisom@jfrl.or.jp
authors: Motoki
authors: M
authors: Ogiso
authors: Ayako
authors: A
authors: Kimura
authors: Masato
authors: M
authors: Chikasou
authors: Maki
authors: M
authors: Saito
authors: Masami
authors: M
authors: Takemoto
authors: Masatoshi
authors: M
authors: Watai
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000378:metabolism*
chemical_list: D000348:Aflatoxins