title: [Effects of simvastatin on proliferation and apoptosis of acute monocytic leukemia cell line SHI-1].
abstract: The purpose of this study was to investigate the effect of simvastatin (SIM) on proliferation and apoptosis of acute monocytic leukemia cell line SHI-1 and its mechanism. Experiments were divided into control and test groups (5 µmol/L, 10 µmol/L, 20 µmol/L SIM groups). The growth inhibitory rate of SHI-1 cells was detected using methyl thiazolyl tetrazolium (MTT) method. The cell cycle distribution and apoptotic rate were measured by using flow cytometry. The expression of BCL-2, caspase-3 mRNA were determined by reverse transcription polymerase chain reaction (RT-PCR). The expression of BCL-2, caspase-3 protein levels were analyzed by Western blot. The results demonstrated that SIM inhibited the growth of SHI-1 cells in time- and does-dependent manners. Cell cycle analysis showed that SHI-1 cells significantly arrested in S phase (p < 0.05) after treating with SIM for 48 hours, as compared with control group. 5 µmol/L SIM in test group significantly blocked cell cycle progression, but can not induce apoptosis. The expressions of BCL-2 mRNA and protein were down-regulated and caspase-3 mRNA and protein were up-regulated along with the increase of SIM concentration (p < 0.05). It is concluded that SIM is able to inhibit proliferation and induce apoptosis of SHI-1 cells, the mechanism may be associated with downregulating the expression of apoptosis-related gene BCL-2, upregulating the expression of caspase-3.
authors: Key Laboratory of Thrombosis and Hemostasis Subordinated to Ministry of Health, Jiangsu lnsititute of Hematology, Suzhou University First Affiliated Hospital, Suzhou 215006, Jiangsu Province, China.
authors: Yan-Fen
authors: YF
authors: Li
authors: Ri
authors: R
authors: Zhang
authors: Xu-Hui
authors: XH
authors: Zhang
authors: Guang-Hua
authors: GH
authors: Chen
authors: Jian-Nong
authors: JN
authors: Cen
authors: Zi-Ling
authors: ZL
authors: Zhu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D019821:Simvastatin / Q000494:pharmacology*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D019821:Simvastatin; C505407:CASP3 protein, human; D053148:Caspase 3