title: [Determination of nequinate and buquinolate in livestock products using liquid chromatography-tandem mass spectrometry].
abstract: We studied the simultaneous determination of nequinate and buquinolate, which are used as feed additives to prevent coccidiosis, by means of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The sample was extracted with acetonitrile, then loaded onto an HLB mini-column with 20% methanol. After clean-up with 20% methanol, the analytes were eluted with acetonitrile-methanol (1 : 1). The coccidiostats in the purified samples were determined using ESI-MRM mode LC-MS/MS with a sample matrix calibration curve. Mean recoveries of nequinate and buquinolate from 8 kinds of livestocks samples (chicken muscle, chicken liver, chicken heart, swine muscle, swine heart, cattle muscle, sheep muscle, egg) were in the range of 89.5% to 108.6%, and the relative standard deviation values were <20% (n=10) at the levels of 0.01 μg/g and 0.05 μg/g, respectively. The limits of quantification of these compounds were 0.001 μg/g in each sample.
authors: Tokyo Metropolitan Institute of Public Health. Takayuki_1_Nakajima@member.metro.tokyo.jp
authors: Takayuki
authors: T
authors: Nakajima
authors: Hiroshi
authors: H
authors: Hayashi
authors: Takeo
authors: T
authors: Sasamoto
authors: Maki
authors: M
authors: Kanda
authors: Tomoko
authors: T
authors: Kusano
authors: Youko
authors: Y
authors: Matsushima
authors: Setsuko
authors: S
authors: Kanai
authors: Kazue
authors: K
authors: Takeba
authors: Toshihiro
authors: T
authors: Nagayama
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D003049:Coccidiostats / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D006912:Hydroxyquinolines / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D015363:Quinolones / Q000032:analysis*; D012756:Sheep; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D003049:Coccidiostats; D006912:Hydroxyquinolines; D015363:Quinolones; C057040:nequinate; C100096:buquinolate