title: Light-directed delivery of nucleic acids.
abstract: A major barrier within the field of non-viral gene therapy toward therapeutic strategies, e.g., tumor therapy, has been lack of appropriate specific delivery strategies to the intended target tissues or cells. In this chapter, we describe a protocol for light-directed delivery of nucleic acids through the use of photochemical internalization (PCI) technology. PCI is based on a photosensitizing compound that localizes to endocytic membranes. Upon illumination, the photosensitizing compound induces damage to the endocytic membranes, resulting in release of endocytosed material, i.e., nucleic acids into cytosol. The main benefit of the strategy described is the possibility for site-specific delivery of nucleic acids to a place of interest.
authors: Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, N-0310, Norway. Sigurd.Boe@rr-research.no
authors: Sigurd
authors: S
authors: Bøe
authors: Lina
authors: L
authors: Prasmickaite
authors: Birgit
authors: B
authors: Engesæter
authors: Eivind
authors: E
authors: Hovig
mesh_terms: D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D004705:Endocytosis / Q000187:drug effects; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008027:Light; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012516:Osteosarcoma / Q000188:drug therapy* / Q000473:pathology; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism*; D026082:Singlet Oxygen; D014162:Transfection
chemical_list: D009696:Nucleic Acids; D017319:Photosensitizing Agents; D026082:Singlet Oxygen