title: Gene expression profiling and its utility in prediction of local relapse after breast-conserving therapy in early breast cancer.
abstract: BACKGROUND
  Local relapse (LR) after breast-conserving therapy (BCT) is not accurately predicted by histological/clinical factors. Gene expression profiling was used here to discover LR-associated transcriptional alterations.
  
  
  METHODS
  Gene expression profiling was carried out of 81 early breast carcinomas obtained from 30 patients who developed a LR (LR(+)) after BCT and 51 who did not (LR(-)). LR(+) and LR(-) samples were matched for known LR risk features.
  
  
  RESULTS
  LR was not associated with a given molecular subtype. Supervised analysis identified a 212-gene signature, which was not validated in independent tumors. No gene set or biological pathway was differentially expressed between LR(+) and LR(-) groups. Twelve published prognostic expression signatures failed to distinguish these groups of carcinomas. The gene expression profiles of 9 cases of LR and the corresponding primary tumors were very similar despite the delivery of radiotherapy.
  
  
  CONCLUSIONS
  In this series, the onset of LR was not predicted by gene expression alterations.
authors: Department of Molecular Oncology, Marseille Cancer Research Center, Paoli Calmettes Institute, UMR891 Inserm, Marseille, France.
authors: Renaud
authors: R
authors: Sabatier
authors: Pascal
authors: P
authors: Finetti
authors: Nathalie
authors: N
authors: Cervera
authors: Agnes
authors: A
authors: Tallet
authors: Mohamed
authors: M
authors: Benchalal
authors: Gilles
authors: G
authors: Houvenaeghel
authors: Jocelyne
authors: J
authors: Jacquemier
authors: Daniel
authors: D
authors: Birnbaum
authors: François
authors: F
authors: Bertucci
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D015894:Genome, Human; D006801:Humans; D015412:Mastectomy, Segmental; D046228:Microarray Analysis; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology / Q000532:radiotherapy / Q000601:surgery*; D011379:Prognosis