title: A transposon-based activation tagging system for gene function discovery in Arabidopsis.
abstract: Activation tagging is a powerful strategy to find new gene functions, especially from genes that are redundant or show lethal knock-out phenotypes. It has been applied using T-DNA or transposons. En/Spm-I/dSpm engineered transposons are efficient activation tags in Arabidopsis. An immobilized transposase source and an enhancer-bearing non-autonomous element are used in combination with positive and negative selectable markers to generate a population of single- or low-copy, stable insertions. This method describes the steps required for selection of parental lines, generation of a population of stable insertions, and gene identification.
authors: Laboratorio Nacional de Genómica para la Biodiversidad (LANGEBIO), Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Irapuato, Guanajuato, México. nmarsch@ira.cinvestav.mx
authors: Nayelli
authors: N
authors: Marsch-Martínez
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D015870:Gene Expression; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D016324:Sequence Tagged Sites
chemical_list: D004251:DNA Transposable Elements