title: Determination of sixteen pesticides in peppers using high-performance liquid chromatography/mass spectrometry.
abstract: A method for the detection and quantification of 16 pesticides: flufenoxuron, fenoxycarb, dimethomorph, acetamiprid, imidacloprid, lufenuron, thiacloprid, thiabendazole, thiophanate-methyl, spinosad, fenbutatin oxide, methoxyfenozide, oxamyl, clothianidin, thiamethoxam and carbendazim has been developed based on high-performance liquid chromatography-mass spectrometry. Pesticide residues were extracted from the samples according to the QuEChERS method which stands for quick, essay, cheap, effective, rugged and safe. Homogenised analytical portions (10 g ± 0.1) of samples of peppers were spiked at two levels (10 and 100 μg kg⁻¹) with a small volume of an appropriate standard mixture solution containing each pesticide. Analyses were performed using electrospray ionization (ESI) and a MSD trap system. Chromatography separation was achieved using a ZORBAX SB-C18 3.5 μm particle size analytical column, 2.1 × 50 mm from Agilent, with gradient elution at a flow-rate of 0.4 mL/min with mobile phases: waters-0.1 % HCOOH-5 mM HCOONH₄ and MeOH-5 mM HCOONH₄. The method has been validated based on the SANCO European Guidelines. Under the optimized conditions the recoveries (n = 7) were in the range 70-110 % with satisfactory precision (CV ≤ 20 %). A linear dynamic range was obtained over a range of concentrations from 10 to 100 μg kg⁻¹ for each of the analytes, with correlation coefficients >0.997.
authors: Instrumental Laboratory, Technological National Center of Canning Industry (CTC), Murcia, Spain.
authors: Ascensión
authors: A
authors: Morales
authors: Irene
authors: I
authors: Ruiz
authors: José
authors: J
authors: Oliva
authors: Alberto
authors: A
authors: Barba
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005060:Europe; D005506:Food Contamination / Q000032:analysis*; D017408:Guidelines as Topic / Q000592:standards; D013058:Mass Spectrometry / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis*; D029222:Piper nigrum / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D010573:Pesticide Residues