title: miR-30b/30d regulation of GalNAc transferases enhances invasion and immunosuppression during metastasis.
abstract: To metastasize, a tumor cell must acquire abilities such as the capacity to colonize new tissue and evade immune surveillance. Recent evidence suggests that microRNAs can promote the evolution of malignant behaviors by regulating multiple targets. We performed a microRNA analysis of human melanoma, a highly invasive cancer, and found that miR-30b/30d upregulation correlates with stage, metastatic potential, shorter time to recurrence, and reduced overall survival. Ectopic expression of miR-30b/30d promoted the metastatic behavior of melanoma cells by directly targeting the GalNAc transferase GALNT7, resulted in increased synthesis of the immunosuppressive cytokine IL-10, and reduced immune cell activation and recruitment. These data support a key role of miR-30b/30d and GalNAc transferases in metastasis, by simultaneously promoting cellular invasion and immunosuppression.
authors: Department of Pathology, New York University Medical Center, NY 10016, USA.
authors: Avital
authors: A
authors: Gaziel-Sovran
authors: Miguel F
authors: MF
authors: Segura
authors: Raffaella
authors: R
authors: Di Micco
authors: Mary K
authors: MK
authors: Collins
authors: Douglas
authors: D
authors: Hanniford
authors: Eleazar
authors: E
authors: Vega-Saenz de Miera
authors: John F
authors: JF
authors: Rakus
authors: John F
authors: JF
authors: Dankert
authors: Shulian
authors: S
authors: Shang
authors: Robert S
authors: RS
authors: Kerbel
authors: Nina
authors: N
authors: Bhardwaj
authors: Yongzhao
authors: Y
authors: Shao
authors: Farbod
authors: F
authors: Darvishian
authors: Jiri
authors: J
authors: Zavadil
authors: Adrian
authors: A
authors: Erlebacher
authors: Lara K
authors: LK
authors: Mahal
authors: Iman
authors: I
authors: Osman
authors: Eva
authors: E
authors: Hernando
mesh_terms: D000818:Animals; D001483:Base Sequence; D049109:Cell Proliferation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D007108:Immune Tolerance / Q000235:genetics*; D016753:Interleukin-10 / Q000378:metabolism; D008545:Melanoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D017350:N-Acetylgalactosaminyltransferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D015854:Up-Regulation; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D016753:Interleukin-10; D035683:MicroRNAs; D017350:N-Acetylgalactosaminyltransferases; D009363:Neoplasm Proteins; D011134:Polysaccharides; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C554019:MIRN130 microRNA, human; C546086:MIRN30b microRNA, human