title: Preliminary investigation of a mice model of Klebsiella pneumoniae subsp. ozaenae induced pneumonia.
abstract: In the present study, we comparatively assessed the pathophysiological mechanisms developed during lung infection of BALB/C female mice infected by an original wild type Klebsiella pneumoniae subsp. ozaenae strain (CH137) or by a referent subspecies K. pneumoniae. subsp. pneumoniae strain (ATCC10031). The mice infected with 2.10⁶ CFU K. p. subsp. pneumoniae (n = 10) showed transient signs of infection and all of them recovered. All of those infected with 1.10⁶ CFU K. p. subsp. ozaenae (n = 10) developed pneumonia within 24 h and died between 48 and 72 h. Few macrophages, numerous polymorphonuclear cells and lymphocytes were observed in their lungs in opposite to K. p. subsp. pneumoniae. In bronchoalveolar lavage, a significant increase in MIP-2, IL-6, KC and MCP-1 levels was only observed in K. p. subsp. ozaenae infected mice whereas high levels of TNF-α were evidenced with the two subspecies. Our findings indicated a lethal effect of a wild type K. p. subsp. ozaenae strain by acute pneumonia reflecting an insufficient alveolar macrophage response. This model might be of a major interest to comparatively explore the pathogenicity of K. p. subsp ozaenae strains and to further explore the physiopathological mechanisms of gram-negative bacteria induced human pneumonia.
authors: Unité de Virologie Médicale et Moléculaire et EA-4303, UFR Médecine, Université Reims Champagne-Ardenne, F-51092 Reims, France.
authors: Fanny
authors: F
authors: Renois
authors: Jérôme
authors: J
authors: Jacques
authors: Thomas
authors: T
authors: Guillard
authors: Hélène
authors: H
authors: Moret
authors: Michel
authors: M
authors: Pluot
authors: Laurent
authors: L
authors: Andreoletti
authors: Christophe
authors: C
authors: de Champs
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015169:Colony Count, Microbial; D016207:Cytokines / Q000032:analysis* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007113:Immunity, Innate; D007710:Klebsiella Infections / Q000276:immunology / Q000382:microbiology / Q000401:mortality / Q000473:pathology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000276:immunology / Q000472:pathogenicity*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016676:Macrophages, Alveolar / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D018410:Pneumonia, Bacterial / Q000276:immunology / Q000382:microbiology / Q000401:mortality / Q000473:pathology*; D013154:Spleen / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D013997:Time Factors
chemical_list: D016207:Cytokines