title: Molecular characterization and expression of dipeptidase 3, a testis-specific membrane-bound dipeptidase: complex formation with TEX101, a germ-cell-specific antigen in the mouse testis.
abstract: We previously established an anti-sperm head auto-monoclonal antibody designated Ts4. The immunoreactivity of this antibody was also observed in other reproduction-related cells, such as testicular germ cells and early embryos, suggesting that the Ts4-recognized molecules might play a role in the reproductive process. However, the molecular characteristics and functions of the antigens warrant further clarification. In this study, we primarily attempted identification of the mAb-recognized molecules within the mouse testis. An immunoprecipitation method, together with liquid chromatography-tandem mass spectrometry, revealed that the testicular immunoprecipitants with Ts4 contained dipeptidase 3 (DPEP3), a member of the membrane-bound dipeptidase family. A Western blot analysis using an anti-DPEP3 polyclonal antibody established in this study showed that this molecule was glycosylated and formed a disulfide-linked homodimer within the testis. Expression of DPEP3 protein was observed in the testicular germ cells, but not in the Sertoli or interstitial cells, or in any other major organs. Although Western blot analysis of testicular proteins separated by two-dimensional SDS-PAGE failed to demonstrate binding of Ts4 to DPEP3, we found that DPEP3 forms complexes with Ts4-immunoreactive molecules, such as TEX101, on the surfaces of spermatocytes, spermatids, and testicular spermatozoa. Based on data showing in the present study, further studies concerning DPEP3 on the testicular germ cells may help to clarify the molecular mechanisms of testicular germ-cell development.
authors: Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba 279-0021, Japan.
authors: Hiroshi
authors: H
authors: Yoshitake
authors: Mitsuaki
authors: M
authors: Yanagida
authors: Mayuko
authors: M
authors: Maruyama
authors: Kenji
authors: K
authors: Takamori
authors: Akiko
authors: A
authors: Hasegawa
authors: Yoshihiko
authors: Y
authors: Araki
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D000954:Antigens, Surface / Q000096:biosynthesis* / Q000276:immunology; D001323:Autoantibodies / Q000737:chemistry; D004150:Dipeptidases / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000276:immunology; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D046912:Multiprotein Complexes / Q000096:biosynthesis* / Q000276:immunology; D009928:Organ Specificity / Q000276:immunology; D011817:Rabbits; D012708:Sertoli Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000954:Antigens, Surface; D001323:Autoantibodies; D058851:GPI-Linked Proteins; D008565:Membrane Proteins; D046912:Multiprotein Complexes; C426726:TEX101 protein, mouse; D004150:Dipeptidases; C475507:Dpep3 protein, mouse