title: [Correction of the alkylnitrite method for the measurement of ethanol in blood: direct experimental evidence].
abstract: A coefficient (multiplier) for the correction of a systemic inaccuracy of ethanol detection in the blood by the alkylnitrite method arising from calibration against an aqueous solution has been improved. The experiment was carried out as a multilaboratory study (with the participation of six laboratories). The value of the coefficient was estimated at 0.82 +/- 0.014 (SD) instead of 0.95 computed earlier (CV = 1.7%). The main factor influencing the value of the coefficient turned out to be the time of preparation of the model ethanol-blood mixture for comparison with the aqueous solution. The coefficient of 0.82 was obtained using fresh blood samples that of 0.93 to 0.96 with blood samples previously stored during 3-10 days. The results of the study suggest different ethanol distribution patterns in vivo and in vitro in the aqueous phase of the blood stored for a few days.
authors: T O
authors: TO
authors: Barinskaia
authors: E M
authors: EM
authors: Salomatin
authors: A V
authors: AV
authors: Smirnov
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D002138:Calibration; D000431:Ethanol / Q000097:blood*; D006801:Humans; D007753:Laboratories / Q000592:standards; D009573:Nitrites / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D009573:Nitrites; D012996:Solutions; D014867:Water; D000431:Ethanol