title: Interference of E2-2-mediated effect in endothelial  cells by FAM96B through its limited expression of  E2-2.
abstract: The basic helix–loop–helix protein E2-2 is known to play a role in quiescence of endothelial cells (ECs). However, it is unclear how the activity of E2-2 is controlled in the cells. In this study, we identified FAM96B as an interaction partner of E2-2. FAM96B interfered with E2-2-mediated effects on luciferase reporter activities. Furthermore, the suppression of vascular endothelial growth factor receptor 2 promoter activity by E2-2 was rescued by the expression of FAM96B in a dose-dependent manner. Interestingly, FAM96B decreased the expression of ectopic and endogenous E2-2 proteins. Mutational analysis revealed that the middle region of FAM96B is required for the limited expression of E2-2 protein. When FAM96B was expressed in ECs, the EC migration, proliferation, and tube formation were potentiated. Taken together, these findings suggest that FAM96B acts as a regulator of E2-2 through the control of its protein expression.
authors: Department of Experimental Pathology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors: Weiwen
authors: W
authors: Yang
authors: Fumiko
authors: F
authors: Itoh
authors: Hirotoshi
authors: H
authors: Ohya
authors: Fukiko
authors: F
authors: Kishimoto
authors: Aya
authors: A
authors: Tanaka
authors: Naoko
authors: N
authors: Nakano
authors: Susumu
authors: S
authors: Itoh
authors: Mitsuyasu
authors: M
authors: Kato
mesh_terms: D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D006224:Cricetinae; D042783:Endothelial Cells / Q000378:metabolism*; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D008667:Metalloproteins; D051379:Mice; D041681:NIH 3T3 Cells; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D058118:Transcription Factor 7-Like 2 Protein / Q000235:genetics / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002352:Carrier Proteins; D008667:Metalloproteins; D009687:Nuclear Proteins; D058118:Transcription Factor 7-Like 2 Protein; D040301:Vascular Endothelial Growth Factor Receptor-2; C569498:CIAO2B protein, human; C544894:TCF7L2 protein, human