title: In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.
abstract: Trypanosoma brucei imports all mitochondrial transfer RNAs (tRNAs) from the cytosol. By using cell lines that allow independent tetracycline-inducible RNA interference and isopropyl-β-D-thiogalactopyranoside-inducible expression of a tagged tRNA, we show that ablation of Tim17 and mitochondrial heat-shock protein 70, components of the inner-membrane protein translocation machinery, strongly inhibits import of newly synthesized tRNAs. These findings, together with previous results in yeast and plants, suggest that the requirement for mitochondrial protein-import factors might be a conserved feature of mitochondrial tRNA import in all systems.
authors: Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
authors: Florence
authors: F
authors: Tschopp
authors: Fabien
authors: F
authors: Charrière
authors: André
authors: A
authors: Schneider
mesh_terms: D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D034443:RNA Transport / Q000502:physiology*; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000494:pharmacology; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D033681:Mitochondrial Membrane Transport Proteins; D020648:Peptide Elongation Factor 1; D000077278:RNA, Mitochondrial; D007544:Isopropyl Thiogalactoside; D012313:RNA; D012343:RNA, Transfer; D025261:Mitochondrial Proton-Translocating ATPases; D013752:Tetracycline