title: [Effect of tanshinone II A on TLR4 and TNF-α of endothelial cells induced by LPS].
abstract: OBJECTIVE
  To observe the influence of tanshinone II A on the expression of toll like receptor 4(TLR4) and tumor necrosis factor-α(TNF-α) in cultured human umbilical veins endothelial cells EA.hy926 induced by LPS, and to investigate the molecular mechanism of tanshinone II A against atherosclerosis(AS).
  
  
  METHODS
  The EA.hy926 cells were cultured in vitro. The mRNA and protein expression of TLR4 were tested by RT-PCR and immunocytochemistry technique respectively. The mRNA and protein expression of TNF-α were detected by RT-PCR and ELISA technique respectively.
  
  
  RESULTS
  The expression of TLR4 and TNF-α were both increased compared to normal group after stimulation of LPS(P<0.05). The expression of TLR4 and TNF-α in tanshinone II A group were obviously inhibited compared to stimulation group(P<0.05).
  
  
  CONCLUSIONS
  The molecular mechanism of tanshinone II A against AS is probably to inhibit the expression of TLR4 and TNF-α.
authors: Liaoning University of Traditional Chinese Medicine, Shenyang, China. jlq-8@163.com
authors: Lian-qun
authors: LQ
authors: Jia
authors: Jun-yi
authors: JY
authors: Feng
authors: Guan-lin
authors: GL
authors: Yang
authors: Wen-na
authors: WN
authors: Chen
authors: Yang
authors: Y
authors: Chen
mesh_terms: D045784:Abietanes / Q000494:pharmacology* / Q000627:therapeutic use; D050197:Atherosclerosis / Q000188:drug therapy; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D045784:Abietanes; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C021751:tanshinone