title: Fully automated one-pot radiosynthesis of O-(2-[18F]fluoroethyl)-L-tyrosine on the TracerLab FX(FN) module.
abstract: BACKGROUND
  An efficient fully automated method for the radiosynthesis of enantiomerically pure O-(2-[(18)F]fluoroethyl)-L-tyrosine ([(18)F]FET) using the GE TracerLab FX(FN) synthesis module via the O-(2-tosyloxyethyl)-N-trityl-L-tyrosine tert-butylester precursor has been developed.
  
  
  METHODS
  The radiolabelling of [(18)F]FET involved a classical [(18)F]fluoride nucleophilic substitution performed in acetonitrile using potassium carbonate and Kryptofix 222, followed by acid hydrolysis using 2N hydrochloric acid.
  
  
  RESULTS
  [(18)F]FET was produced in 35±5% (n=22) yield non-decay-corrected (55±5% decay-corrected) and with radiochemical and enantiomeric purity of >99% with a specific activity of >90 GBq/μmol after 63 min of radiosynthesis including HPLC purification and formulation.
  
  
  CONCLUSIONS
  The automated radiosynthesis provides high and reproducible yields suitable for routine clinical use.
authors: LifeSciences, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC NSW 2232, Sydney, Australia. bts@ansto.gov.au
authors: Thomas
authors: T
authors: Bourdier
authors: Ivan
authors: I
authors: Greguric
authors: Peter
authors: P
authors: Roselt
authors: Tim
authors: T
authors: Jackson
authors: Jane
authors: J
authors: Faragalla
authors: Andrew
authors: A
authors: Katsifis
mesh_terms: D001331:Automation; D007202:Indicators and Reagents / Q000737:chemistry; D007753:Laboratories; D011786:Quality Control; D011854:Radiochemistry / Q000295:instrumentation* / Q000592:standards; D015203:Reproducibility of Results; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; C117289:O-(2-fluoroethyl)tyrosine; D014443:Tyrosine