title: Design and characterization of calcium alginate microparticles coated with polycations as protein delivery system.
abstract: Bovine serum albumin (BSA) loaded calcium alginate microparticles (MPs) produced in this study by a w/o emulsification and external gelation method exhibited spherical and fairly smooth and porous morphology with 1.052 ± 0.057 µm modal particle size. The high permeability of the calcium alginate hydrogel lead to a potent burst effect and too fast protein release. To overcome these problems, MPs were coated with polycations, such as chitosan, poly-L-lysine and DEAE-dextran. Our results demonstrated that coated MPs showed slower release and were able to significantly reduce the release of BSA in the first hour. Therefore, this method can be applied to prepare coated alginate MPs which could be an optimal system for the controlled release of biotherapeutic molecules. Nevertheless, further studies are needed to optimize delivery properties which could provide a sustained release of proteins.
authors: NanoBioCel Group, Laboratory of Pharmaceutics, School of Pharmacy, University of the Basque Country, Vitoria, Spain.
authors: J
authors: J
authors: Zarate
authors: L
authors: L
authors: Virdis
authors: G
authors: G
authors: Orive
authors: M
authors: M
authors: Igartua
authors: R M
authors: RM
authors: Hernández
authors: J L
authors: JL
authors: Pedraz
mesh_terms: D000464:Alginates / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D003637:DEAE-Dextran / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D015195:Drug Design; D004655:Emulsions / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D020723:Glucuronic Acid / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D010316:Particle Size; D011073:Polyamines / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D000071228:Polyelectrolytes; D011107:Polylysine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000464:Alginates; D048271:Chitosan; D020099:Coated Materials, Biocompatible; D003637:DEAE-Dextran; D004655:Emulsions; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; D012710:Serum Albumin, Bovine; C051546:alginate-polylysine-alginate