title: In vitro activity against Staphylococcus aureus of a novel antimicrobial agent, PRF-119, a recombinant chimeric bacteriophage endolysin.
abstract: Antistaphylococcal activity of the novel chimeric endolysin PRF-119 was evaluated with the microdilution method. The MIC(50) and MIC(90) of 398 methicillin-susceptible Staphylococcus aureus isolates were 0.098 μg/ml and 0.391 μg/ml, respectively (range, 0.024 to 0.780 μg/ml). Both the MIC(50) and MIC(90) values of 776 methicillin-resistant S. aureus isolates were 0.391 μg/ml (range, 0.024 to 1.563 μg/ml). All 192 clinical isolates of coagulase-negative staphylococci exhibited MIC values of >50 μg/ml. In conclusion, PRF-119 exhibited very good activity specifically against S. aureus.
authors: University Hospital of Münster, Institute of Medical Microbiology, D-48149 Münster, Germany.
authors: Evgeny A
authors: EA
authors: Idelevich
authors: Christof
authors: C
authors: von Eiff
authors: Alexander W
authors: AW
authors: Friedrich
authors: Domenico
authors: D
authors: Iannelli
authors: Guoqing
authors: G
authors: Xia
authors: Georg
authors: G
authors: Peters
authors: Andreas
authors: A
authors: Peschel
authors: Ingrid
authors: I
authors: Wanninger
authors: Karsten
authors: K
authors: Becker
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D011994:Recombinant Proteins; D010450:Endopeptidases; C001143:endolysin