title: LC-ESI/MS/MS method for rapid screening and confirmation of 44 exogenous anabolic steroids in human urine.
abstract: A sensitive and rapid method based on liquid chromatography-triple-quadrupole tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI) has been developed and validated for the screening and confirmation of 44 exogenous anabolic steroids (29 parent steroids and 15 metabolites) in human urine. The method involves an enzymatic hydrolysis, liquid-liquid extraction, and detection by LC-MS/MS. A triple-quadrupole mass spectrometer was operated in positive ESI mode with selected reaction monitoring (SRM) mode for the screening and product ion scan mode for the confirmation. The protonated molecular ions were used as precursor ions for the SRM analysis and product ion scan. The intraday and interday precisions of the target analytes at concentrations of the minimum required performance levels for the screening were 2-14% and 2-15%, respectively. The limits of detection for the screening and confirmation method were 0.1-10 ng/mL and 0.2-10 ng/mL, respectively, for 44 steroids. This method was successfully applied to analysis of urine samples from suspected anabolic steroid abusers.
authors: Doping Control Center, Korea Institute of Science and Technology, Seoul, Korea.
authors: Byoung Wook
authors: BW
authors: Jeon
authors: Hye Hyun
authors: HH
authors: Yoo
authors: Eun Sook
authors: ES
authors: Jeong
authors: Ho Jun
authors: HJ
authors: Kim
authors: Changbae
authors: C
authors: Jin
authors: Dong Hyun
authors: DH
authors: Kim
authors: Jaeick
authors: J
authors: Lee
mesh_terms: D045930:Anabolic Agents / Q000652:urine*; D002853:Chromatography, Liquid; D006801:Humans; D057230:Limit of Detection; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013256:Steroids / Q000652:urine*; D013997:Time Factors; D016482:Urinalysis / Q000379:methods* / Q000592:standards*
chemical_list: D045930:Anabolic Agents; D013256:Steroids