title: Influenza virus hemagglutinin: a model for protein N-glycosylation in recombinant Escherichia coli.
abstract: BACKGROUND
  The hemagglutinin molecule of influenza virus is considered as an ideal model to study biological processes as well as the effect of glycosylation on the function of glycoproteins.
  
  
  OBJECTIVE
  The large subunit of the influenza virus A/New Caledonia/20/99 (H1N1) hemagglutinin (HA1) was expressed in recombinant Escherichia coli containing the glycosylation system of Campylobacter jejuni. This viral glycoprotein contains glycosylation motifs recognized by prokaryotic and eukaryotic oligosaccharyltransferases.
  
  
  METHODS
  In order to express the hemagglutinin large subunit gene, the gene was amplified using reverse transcription polymerase chain reaction (RT-PCR), and it was cloned in pET22b for periplasmic expression.
  
  
  RESULTS
  Western blotting and lectin blotting bands confirmed glycosylation of the HA1 in recombinant E. coli.
  
  
  CONCLUSIONS
  Such a successful accomplishment of hemagglutinin expression in recombinant E. coli can be used to construct carbohydrates in hemagglutinin molecules of different strains in order to produce effective antigens for vaccine and rapid diagnostic kits against new emerging viruses.
authors: Department of Virology (Influenza Unit), Pasteur Institute of Iran, Tehran, Iran.
authors: Behrokh
authors: B
authors: Farahmand
authors: Mahvash
authors: M
authors: Khodabandeh
authors: Fereidoun
authors: F
authors: Mahboudi
authors: Fatemeh
authors: F
authors: Fotouhi
authors: Maryam
authors: M
authors: Saleh
authors: Farzaneh
authors: F
authors: Barkhordari
authors: Mansoureh
authors: M
authors: Tabatabaian
authors: Farnoush Parsaie
authors: FP
authors: Nasab
authors: Masoumeh Tavassoti
authors: MT
authors: Kheiri
mesh_terms: D016123:Campylobacter jejuni / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000378:metabolism*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011994:Recombinant Proteins