title: Entomological study of chikungunya infections in the State of Kelantan, Malaysia.
abstract: OBJECTIVE
  Chikungunya infection has become a public health threat in Malaysia since the 2008 nationwide outbreaks. Aedes albopictus Skuse has been identified as the chikungunya vector in Johor State during the outbreaks. In 2009, several outbreaks had been reported in the State of Kelantan. Entomological studies were conducted in Kelantan in four districts, namely Jeli, Tumpat, Pasir Mas and Tanah Merah to identify the vector responsible for the virus transmission.
  
  
  METHODS
  CHIKV cases records were obtained from State Health Department, Kelantan and localities involved were identified. Larva survey was conducted to collect the immature mosquito stages. Modified aspirators were used to collect the adult mosquitoes. All samples on dry ice were transferred to laboratory and the presence of the virus was detected using reverse transcriptase PCR.
  
  
  RESULTS
  A total of 1,245 mosquito larvae were collected during larval survey and 2,019 adult mosquitoes were collected using aspirator. From these collections, 640 mosquito pools were tested for the presence of CHIKV by RT-PCR but none found positive. Ae. albopictus was the most abundant mosquito collected, followed by Culex sp., Armigeres sp. and Anopheles sp. A total of 2, 814 artificial containers were inspected during the study.
  
  
  CONCLUSIONS
  Since none of the mosquito samples was found to be positive for chikungunya virus, the vector(s) of chikungunya virus in these localities could not be identified.
authors: Institute for Medical Research, Ministry of Health, Kuala Lumpur, Malaysia. watynom@yahoo.com
authors: H
authors: H
authors: Rozilawati
authors: A Y
authors: AY
authors: Faudzi
authors: A A Siti
authors: AA
authors: Rahidah
authors: A H Nor
authors: AH
authors: Azlina
authors: A G
authors: AG
authors: Abdullah
authors: N M
authors: NM
authors: Amal
authors: H Wan
authors: HW
authors: Mansor
authors: H
authors: H
authors: Hani
authors: Y
authors: Y
authors: Apandi
authors: Faezah
authors: F
authors: Noor
authors: Norziyana
authors: W A
authors: WA
authors: Nazni
authors: J
authors: J
authors: Zairi
authors: H L
authors: HL
authors: Lee
mesh_terms: D018354:Alphavirus Infections / Q000453:epidemiology* / Q000635:transmission*; D000818:Animals; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000235:genetics / Q000472:pathogenicity*; D009033:Culicidae / Q000502:physiology / Q000821:virology*; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D007814:Larva / Q000821:virology; D008296:Malaysia / Q000453:epidemiology; D020133:Reverse Transcriptase Polymerase Chain Reaction