title: Molecular characterization of measles virus strains causing subacute sclerosing panencephalitis in France in 1977 and 2007.
abstract: Measles virus strains from two subacute sclerosing panencephalitis (SSPE) cases diagnosed in 1977 (Laine strain) and in 2007 (Hoedts strain) were studied. Phylogenetic analysis based on C-terminal part of the nucleoprotein and the entire H gene showed that Hoedts strain, circulating in France presumably in the 1980s, belonged to genotype C2. However, Laine strain, suspected to have circulated between 1940s and 1960s, could not be assigned to any known measles virus genotypes. Sequences analysis of the Laine strain suggested that it originated from a measles virus that may have circulating at the same period as the Edmonston strain. The analysis of the whole genome of both SSPE strains revealed biased hypermutations in M, F, and H gene. Some of these mutations like the L165P found in the M protein sequence of the Laine strain, the amino acid position 94, where a mutation M94V was found in the F protein sequence of the Hoedts strain are known to play an important role in the glycoprotein interaction and to impair the ability of measles virus strain to produce cell-free infectious viral particles.This is the first study on molecular characterization of the entire coding region of measles virus isolated from SSPE cases in France.
authors: National Reference Center for Measles, Lyon, France; Immunobiology of Viral Infections, Inserm, Lyon, France; Ecole Normale Supérieure de Lyon, Lyon, France; IFR128 BioSciences Lyon-Gerland Lyon-Sud, Lyon, France; University of Lyon, Lyon, France.
authors: Emilie
authors: E
authors: Moulin
authors: Vanda
authors: V
authors: Beal
authors: Damien
authors: D
authors: Jeantet
authors: Branka
authors: B
authors: Horvat
authors: T Fabian
authors: TF
authors: Wild
authors: Diane
authors: D
authors: Waku-Kouomou
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D005602:France; D005814:Genes, Viral; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008459:Measles virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D013344:Subacute Sclerosing Panencephalitis / Q000821:virology*; D014760:Viral Fusion Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus