title: Selective removal of promoter nucleosomes by the RSC chromatin-remodeling complex.
abstract: Purified chromatin rings, excised from the PHO5 locus of Saccharomyces cerevisiae in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, namely that promoter specificity resides primarily in the nucleosomes rather than in the remodeling complex that acts upon them.
authors: Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA. lorch@stanford.edu
authors: Yahli
authors: Y
authors: Lorch
authors: Joachim
authors: J
authors: Griesenbeck
authors: Hinrich
authors: H
authors: Boeger
authors: Barbara
authors: B
authors: Maier-Davis
authors: Roger D
authors: RD
authors: Kornberg
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000235:genetics*; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000502:physiology*; D006655:Histone Deacetylases / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; D009707:Nucleosomes; C103337:RSC complex, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000135:Acid Phosphatase; C488916:PHO5 protein, S cerevisiae; D006655:Histone Deacetylases