title: Heterofucan from Sargassum filipendula induces apoptosis in HeLa cells.
abstract: Fucan is a term used to denominate a family of sulfated polysaccharides rich in sulfated l-fucose. Heterofucan SF-1.5v was extracted from the brown seaweed Sargassum filipendula by proteolytic digestion followed by sequential acetone precipitation. This fucan showed antiproliferative activity on Hela cells and induced apoptosis. However, SF-1.5v was not able to activate caspases. Moreover, SF-1.5v induced glycogen synthase kinase (GSK) activation, but this protein is not involved in the heterofucan SF-1.5v induced apoptosis mechanism. In addition, ERK, p38, p53, pAKT and NFκB were not affected by the presence of SF-1.5v. We determined that SF-1.5v induces apoptosis in HeLa mainly by mitochondrial release of apoptosis-inducing factor (AIF) into cytosol. In addition, SF-1.5v decreases the expression of anti-apoptotic protein Bcl-2 and increased expression of apoptogenic protein Bax. These results are significant in that they provide a mechanistic framework for further exploring the use of SF-1.5v as a novel chemotherapeutics against human cervical cancer.
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.|Federal Institute of Education, Science and Technology of Rio Grande do Norte (IFRN), Santa Cruz-RN, Brazil.
authors: Leandro
authors: L
authors: Silva Costa
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Cinthia Beatrice
authors: CB
authors: Silva Telles
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Ruth
authors: R
authors: Medeiros Oliveira
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Leonardo Thiago
authors: LT
authors: Duarte Barreto Nobre
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Nednaldo
authors: N
authors: Dantas-Santos
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Rafael
authors: R
authors: Barros Gomes Camara
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Mariana
authors: M
authors: Santana Santos Pereira Costa
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Jailma
authors: J
authors: Almeida-Lima
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Raniere Fagundes
authors: RF
authors: Melo-Silveira
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Ivan Rui
authors: IR
authors: Lopes Albuquerque
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Edda Lisboa
authors: EL
authors: Leite
authors: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN, Brazil.
authors: Hugo Alexandre
authors: 0000-0003-2252-1221
authors: HA
authors: Oliveira Rocha
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051033:Apoptosis Inducing Factor / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005643:Fucose / Q000737:chemistry*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D011134:Polysaccharides; D005643:Fucose