title: Liquid chromatography-tandem mass spectrometry for quantification of lacosamide, an antiepileptic drug, in rat plasma and its application to pharmacokinetic study.
abstract: A rapid, simple and sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) was developed for the determination of an antiepileptic drug, lacosamide, in rat plasma. The method involves the addition of acetonitrile and internal standard solution to plasma samples, followed by centrifugation. An aliquot of the supernatant was diluted with water and directly injected into the LC/MS/MS system. The separations were performed on column packed with octadecylsilica (5 µm, 2.0 × 50 mm) with 0.1% formic acid and acetonitrile as mobile phase, and the detection was performed on tandem mass spectrometry by the multiple-reaction monitoring via an electrospray ionization source. The standard curve was linear over the concentration range from 0.3 to 1000 ng/mL. The lower limit of quantification was 0.3 ng/mL using 50 μL of rat plasma sample. The intra- and inter-assay precision and accuracy were found to be less than 11.7 and 8.8%, respectively. The developed analytical method was successfully applied to the pharmacokinetic study of lacosamide in rats.
authors: Life Science R&D Park, SK Holdings Co. Ltd, Daejeon, 305-712, Korea.
authors: Soo-Jin
authors: SJ
authors: Kim
authors: Tae-Sung
authors: TS
authors: Koo
authors: Dong-Jin
authors: DJ
authors: Ha
authors: Myoungki
authors: M
authors: Baek
authors: Sang-Kil
authors: SK
authors: Lee
authors: Dong-Soo
authors: DS
authors: Shin
authors: Hongsik
authors: H
authors: Moon
mesh_terms: D000081:Acetamides / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000078334:Lacosamide; D057230:Limit of Detection; D051381:Rats; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000081:Acetamides; D000927:Anticonvulsants; D000078334:Lacosamide