title: Molecular identification of Taenia tapeworms by Cox1 gene in Koh Kong, Cambodia.
abstract: We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
authors: Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine, Cheongju 361-711, Korea.
authors: Hyeong-Kyu
authors: HK
authors: Jeon
authors: Tai-Soon
authors: TS
authors: Yong
authors: Woon-Mok
authors: WM
authors: Sohn
authors: Jong-Yil
authors: JY
authors: Chai
authors: Sung-Jong
authors: SJ
authors: Hong
authors: Eun-Taek
authors: ET
authors: Han
authors: Hoo-Gn
authors: HG
authors: Jeong
authors: Tep
authors: T
authors: Chhakda
authors: Muth
authors: M
authors: Sinuon
authors: Duong
authors: D
authors: Socheat
authors: Keeseon S
authors: KS
authors: Eom
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002160:Cambodia; D002648:Child; D051545:Cyclooxygenase 1 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D041202:Taenia saginata / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D041201:Taenia solium / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D013622:Taeniasis / Q000469:parasitology*; D055815:Young Adult
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D015801:Helminth Proteins; D051545:Cyclooxygenase 1