title: Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology.
abstract: A new suspension array technology is proposed for the simultaneous quantitative determination of five antibiotics-tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol in milk. A novel treatment of milk samples for suspension array with diethyl ether was performed which greatly reduced the interference of the disturbing components in milk on the reaction results with no significant effect on detection sensitivity. Compared with using biotin labeled monoclonal antibody, using of secondary antibody-biotin make the detection sensitivity further improved. The minimum detectable concentration in samples of tylosin, tetracycline, gentamicin, streptomycin, and chloramphenicol were 0.3, 1.5, 4, 20, and 25 ng/ml, respectively, and the working ranges of samples were 6-400, 7-300, 8-200, 90-3000 and 70-8000 ng/ml, respectively. The mean recovery was 89.38-113.73% with a standard deviation within 16.62%. The suspension assay technology is powerful for the fast quantitative analysis of multi-antibiotics residue in milk.
authors: Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Institute of Health and Environmental Medicine, Tianjin 300050, PR China.
authors: Pu
authors: P
authors: Su
authors: Nan
authors: N
authors: Liu
authors: Maoxiang
authors: M
authors: Zhu
authors: Baoan
authors: B
authors: Ning
authors: Ming
authors: M
authors: Liu
authors: Zhihua
authors: Z
authors: Yang
authors: Xiujie
authors: X
authors: Pan
authors: Zhixian
authors: Z
authors: Gao
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001710:Biotin / Q000378:metabolism; D002021:Buffers; D002138:Calibration; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D002021:Buffers; D001710:Biotin