title: Genome-wide transcriptional profiling analysis of all trans retinoic acid-treated tongue carcinoma SCC-9 cells.
abstract: BACKGROUND
  All trans retinoic acid (ATRA) is used as standard of care in promyelocytic leukemia. Not much is known about the gene expression profile in ATRA-treated tongue cancer cells. We performed a genome-wide transcriptional profiling of ATRA-treated tongue cancer cells to understand the pathways that mediate ATRA action in tongue cancer.
  
  
  METHODS
  We measured the effects of ATRA on the proliferation of SCC-9 human tongue carcinoma cells. The differential gene expression profile was measured by microarray analysis of untreated and ATRA-treated cells and expression of key genes was validated by real-time RT-PCR.
  
  
  RESULTS
  ATRA treatment (24 and 48 hr) significantly inhibited SCC-9 cell proliferation in a dose-dependent manner. SCC-9 cells treated for 48 hr with ATRA showed upregulation of 276 genes, including ANGPTL4, GDF15, ICAM1 and TUSC4, and downregulation of 43 genes, including CXCL10. Validation by real-time PCR showed a significant upregulation of intracellular adhesion molecule 1 (ICAM1) and downregulation of CXCL10 and IL32.
  
  
  CONCLUSIONS
  ATRA had an anti-tumor effect in tongue cancer cells. This effect is likely mediated via upregulation of ICAM1 and downregulation of CXCL10 and IL32.
authors: Department of Oral Medicine, Guanghua School of Stomatology and Institute of Stomatological Research, Sun Yat-Sen University, Guangzhou, Guangdong, People's Republic of China.
authors: Yaohui
authors: Y
authors: Dai
authors: Juan
authors: J
authors: Wang
authors: Juan
authors: J
authors: Xia
authors: Yun
authors: Y
authors: Hong
authors: Na
authors: N
authors: Chen
authors: Bin
authors: B
authors: Cheng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D055106:Genome-Wide Association Study; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D016014:Linear Models; D020411:Oligonucleotide Array Sequence Analysis; D014062:Tongue Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C496971:IL32 protein, human; D007378:Interleukins; D018799:Intercellular Adhesion Molecule-1; D014212:Tretinoin