title: Multicolor, one- and two-photon imaging of enzymatic activities in live cells with fluorescently Quenched Activity-Based Probes (qABPs).
abstract: Fluorescence imaging provides an indispensable way to locate and monitor biological targets within complex and dynamic intracellular environments. Of the various imaging agents currently available, small molecule-based probes provide a powerful tool for live cell imaging, primarily due to their desirable properties, including cell permeability (as a result of their smaller sizes), chemical tractability (e.g., different molecular structures/designs can be installed), and amenability to imaging a wide variety of biological events. With a few exceptions, most existing small molecule probes are however not suitable for in vivo bioimaging experiments in which high-resolution studies of enzyme activity and localization are necessary. In this article, we reported a new class of fluorescently Quenched Activity-Based Probes (qABPs) which are highly modular, and can sensitively image (through multiple enzyme turnovers leading to fluorescence signal amplification) different types of enzyme activities in live mammalian cells with good spatial and temporal resolution. We have also incorporated two-photon dyes into our modular probe design, enabling for the first time activity-based, fluorogenic two-photon imaging of enzyme activities. This, hence, expands the repertoire of 'smart', responsive probes currently available for live cell bioimaging experiments.
authors: Department of Chemistry, National University of Singapore, Singapore 117543.
authors: Mingyu
authors: M
authors: Hu
authors: Lin
authors: L
authors: Li
authors: Hao
authors: H
authors: Wu
authors: Ying
authors: Y
authors: Su
authors: Peng-Yu
authors: PY
authors: Yang
authors: Mahesh
authors: M
authors: Uttamchandani
authors: Qing-Hua
authors: QH
authors: Xu
authors: Shao Q
authors: SQ
authors: Yao
mesh_terms: D016227:Benzoquinones / Q000737:chemistry; D057056:Cysteine Proteases / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D015394:Molecular Structure; D017785:Photons; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; C004532:quinone; D017027:Protein Tyrosine Phosphatases; D057056:Cysteine Proteases