title: SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a powerful tool for deciphering the protein-DNA interaction space.
abstract: DNA-binding proteins, including transcription factors, play essential roles in many biological processes. The identification of the DNA sequences to which these proteins bind is a first, yet still challenging, step for determining their functions. SELEX provides an excellent tool for deciphering protein DNA-binding sequence specificity, and it has been widely adopted for addressing fundamental biological questions. SELEX is an experimental procedure that involves the progressive selection, from a large combinatorial double-stranded oligonucleotide library, of DNA ligands with variable DNA-binding affinities and specificities by repeated rounds of partition and amplification. In this chapter, we describe a SELEX protocol that we have successfully applied to both plant and animal MYB transcription factors.
authors: Department of Plant Cellular and Molecular Biology, Plant Biotechnology Center, The Ohio State University, Columbus, OH, USA. chenglin.ch@gmail.com
authors: Chenglin
authors: C
authors: Chai
authors: Zidian
authors: Z
authors: Xie
authors: Erich
authors: E
authors: Grotewold
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D018744:DNA, Plant / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D008024:Ligands; D016133:Polymerase Chain Reaction; D011485:Protein Binding / Q000235:genetics; D052156:SELEX Aptamer Technique / Q000379:methods*; D014157:Transcription Factors / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D008024:Ligands; C500589:Myb protein, Arabidopsis; D014157:Transcription Factors