title: A kinetic spectrophotometric method for simultaneous determination of phenol and its three derivatives with the aid of artificial neural network.
abstract: A novel kinetic spectrophotometric method was developed for determination of pyrocatechol, resorcin, hydroquinone and phenol based on their inhibitory effect on the oxidation of Rhodamine B (RhB) in acid medium at pH=3.0. A linear relationship was observed between the inhibitory effect and the concentrations of the compounds. The absorbance associated with the kinetic reactions was monitored at the maximum wavelength of 557nm. The effects of different parameters such as pH, concentration of RhB and KBrO(3), and temperature of the reaction were investigated and optimum conditions were established. The linear ranges were 0.22-3.30, 0.108-0.828, 0.36-3.96 and 1.52-19.76μg mL(-1) for pyrocatechol, resorcin, hydroquinone and phenol, respectively, and their corresponding detection limits were 0.15, 0.044, 0.16 and 0.60μg mL(-1). The measured data were processed by several chemometrics methods, such as principal component regression (PCR), partial least squares (PLS) and artificial neural network (ANN), and a set of synthetic mixtures of these compounds was used to verify the established models. It was found that the prediction ability of PLS, PCR and RBF-ANN was similar, however, the RBF-ANN model did perform somewhat better than the other methods. The proposed method was also applied satisfactorily for the simultaneous determination of pyrocatechol, resorcin, hydroquinone and phenol in real water samples.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. ynni@ncu.edu.cn
authors: Yongnian
authors: Y
authors: Ni
authors: Zhenzhen
authors: Z
authors: Xia
authors: Serge
authors: S
authors: Kokot
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D016571:Neural Networks, Computer; D019800:Phenol / Q000032:analysis* / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D019800:Phenol