title: Ordered nanopattern arrangement of gold nanoparticles on β-sheet peptide templates through nucleobase pairing.
abstract: We have demonstrated a unique method for rational arrangement of gold (Au) nanoparticles on a β-sheet peptide template through nucleobase pairing. For the template, the 16-mer peptide 1 was synthesized, which is based on an alternating amphiphilic sequence of Asp-Leu. Here Leu at the sixth position is replaced by thymine-modified Lys, and a polyethylene glycol chain is introduced to the C-terminus. The surface of Au nanoparticles was modified with the complementary adenyl group. Peptide 1 formed a stable β-sheet monolayer at the air/water interface under an appropriate surface pressure. The monolayer film transferred onto a mica surface by the Langmuir-Blodgett method showed a linearly striped pattern with 6.1 nm average stripe width and 6 nm average interval between stripes, derived from β-sheet assembly. The adenine-bound Au nanoparticles were successfully immobilized on the thymine-bound template through a complementary adenine-thymine hydrogen bonding pair. Interestingly, linear assembly structures of the Au nanoparticles were observed, thus being successfully reproduced by the original striped pattern of the template of 1. Our method might readily fabricate Au materials with our desirable 2D pattern through fine-tuning of β-sheet sequence and nucleobase position.
authors: Department of Frontier Materials, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Nagoya, Aichi, 466-8555, Japan.
authors: Takayuki
authors: T
authors: Nonoyama
authors: Masayoshi
authors: M
authors: Tanaka
authors: Yoshihito
authors: Y
authors: Inai
authors: Masahiro
authors: M
authors: Higuchi
authors: Takatoshi
authors: T
authors: Kinoshita
mesh_terms: D000327:Adsorption; D000388:Air; D000538:Aluminum Silicates / Q000737:chemistry; D020029:Base Pairing; D006046:Gold / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D014867:Water / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D010455:Peptides; D014867:Water; D006046:Gold; C011934:mica