title: Local inflammatory markers and systemic endotoxin in aggressive periodontitis.
abstract: While much research has focused on local and systemic factors contributing to periodontal disease, little is known regarding mechanisms linking these factors. We have previously reported a systemic hyper-inflammatory response to bacterial endotoxin in localized aggressive periodontitis (LAP). The objectives of this study were to delineate cyto/chemokines in gingival crevicular fluid (GCF) and evaluate systemic levels of endotoxin associated with LAP. Clinical parameters, GCF, and peripheral blood were collected from: 34 LAP, 10 healthy siblings, and nine healthy unrelated control individuals. Cyto/chemokines were quantified in GCF, systemic endotoxin levels were quantified in plasma, and correlation analysis was performed among all parameters. Nine mediators were elevated in LAP diseased sites as compared with healthy sites (TNFα, INFγ, IL1β, IL2, IL6, IL10, Il12p40, GMCSF, and MIP1α, p < 0.001), while MCP1, IL4, and IL8 were elevated in healthy sites (p < 0.01). Four- to five-fold-higher endotoxin levels were detected in LAP plasma compared with that from healthy participants (p < 0.0001), which correlated with all clinical parameters and most cyto/chemokines analyzed. In conclusion, higher systemic levels of endotoxin were found in LAP, which correlates with an exacerbated local inflammatory response and clinical signs of disease. (Clinicaltrials.gov number, NCT01330719).
authors: Department of Periodontology, University of Florida College of Dentistry, PO Box 100434, Gainesville, FL 32610-0434, USA. lshaddox@dental.ufl.edu
authors: L M
authors: LM
authors: Shaddox
authors: J
authors: J
authors: Wiedey
authors: N L
authors: NL
authors: Calderon
authors: I
authors: I
authors: Magnusson
authors: E
authors: E
authors: Bimstein
authors: J A
authors: JA
authors: Bidwell
authors: E F
authors: EF
authors: Zapert
authors: I
authors: I
authors: Aukhil
authors: S M
authors: SM
authors: Wallet
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000032:analysis; D000293:Adolescent; D001741:Black or African American; D010520:Aggressive Periodontitis / Q000097:blood / Q000175:diagnosis*; D015415:Biomarkers; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000032:analysis*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D007378:Interleukins / Q000032:analysis; D008070:Lipopolysaccharides / Q000097:blood*; D008297:Male; D012044:Regression Analysis; D018709:Statistics, Nonparametric; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; D016207:Cytokines; D007378:Interleukins; D008070:Lipopolysaccharides; C493277:MAPKAP1 protein, human; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor