title: Plasma pentraxin-3 as a marker of bioincompatibility in hemodialysis patients.
abstract: BACKGROUND
  Hemodialysis (HD)-induced inflammation has a pathogenetic role in patients with end-stage renal disease (ESRD). The aim of the present study was to assess whether pentraxin-3 (PTX3) could be a reliable biomarker of HD-induced inflammation and of membrane biocompatibility.
  
  
  METHODS
  We prospectively enrolled 31 HD patients. Blood samples for determining PTX3, C-reactive protein (CRP), leukocytes and neutrophils were drawn from the arterial needle, before dialysis after the long dialysis-free interval (time 0), at the end of the index session (time 1) and before the next dialysis session (time 2). In 22 of 31 patients, 30 minutes after start of dialysis, PTX3 and CRP plasma levels were measured in blood collected from both the arterial and venous lines (time A - time V) of the dialyzer. In 7 of 22 patients intracellular PTX3 levels in neutrophils were measured at the end of session.
  
  
  RESULTS
  PTX3 venous levels were significantly increased at the end of the index session compared with baseline and in blood samples drawn from the venous line compared with the arterial line of the dialyzer. At time 1, a reduction of intracellular PTX3 in neutrophils was noticed. In contrast, CRP plasma levels were stable during the HD session.
  
  
  CONCLUSIONS
  Our findings suggest that PTX3, which is rapidly produced by several cell types and released by neutrophils upon stimulation, could be a biomarker of HD-induced inflammation and of blood-membrane bioincompatibility.
authors: Nephrology Unit, Humanitas Clinical Institute, Rozzano, Milan, Italy. silvia.oldani@humanitas.it
authors: Silvia
authors: S
authors: Oldani
authors: Silvia
authors: S
authors: Finazzi
authors: Barbara
authors: B
authors: Bottazzi
authors: Cecilia
authors: C
authors: Garlanda
authors: Eleonora
authors: E
authors: Baldassarre
authors: Serenella
authors: S
authors: Valaperta
authors: Ivan
authors: I
authors: Cuccovillo
authors: Marco
authors: M
authors: Albini
authors: Maurice
authors: M
authors: Child
authors: Alessandro
authors: A
authors: Montanelli
authors: Giorgio
authors: G
authors: Graziani
authors: Salvatore
authors: S
authors: Badalamenti
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001672:Biocompatible Materials / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000378:metabolism*; D005260:Female; D017583:Hemodiafiltration / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000628:therapy; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D011446:Prospective Studies; D006435:Renal Dialysis / Q000009:adverse effects*; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D000099153:Pentraxins
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins