title: Kinetic and chemical mechanisms of homocitrate synthase from Thermus thermophilus.
abstract: The homocitrate synthase from Thermus thermophilus (TtHCS) is a metal-activated enzyme with either Mg(2+) or Mn(2+) capable of serving as the divalent cation. The enzyme exhibits a sequential kinetic mechanism. The mechanism is steady state ordered with α-ketoglutarate (α-Kg) binding prior to acetyl-CoA (AcCoA) with Mn(2+), whereas it is steady state random with Mg(2+), suggesting a difference in the competence of the E·Mn·α-Kg·AcCoA and E·Mg·α-Kg·AcCoA complexes. The mechanism is supported by product and dead-end inhibition studies. The primary isotope effect obtained with deuterioacetylCoA (AcCoA-d(3)) in the presence of Mg(2+) is unity (value 1.0) at low concentrations of AcCoA, whereas it is 2 at high concentrations of AcCoA. Data suggest the presence of a slow conformational change induced by binding of AcCoA that accompanies deprotonation of the methyl group of AcCoA. The solvent kinetic deuterium isotope effect is also unity at low AcCoA, but is 1.7 at high AcCoA, consistent with the proposed slow conformational change. The maximum rate is pH independent with either Mg(2+) or Mn(2+) as the divalent metal ion, whereas V/K(α-Kg) (with Mn(2+)) decreases at low and high pH giving pK values of about 6.5 and 8.0. Lysine is a competitive inhibitor that binds to the active site of TtHCS, and shares some of the same binding determinants as α-Kg. Lysine binding exhibits negative cooperativity, indicating cross-talk between the two monomers of the TtHCS dimer. Data are discussed in terms of the overall mechanism of TtHCS.
authors: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019.
authors: Vidya Prasanna
authors: VP
authors: Kumar
authors: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019.
authors: Ann H
authors: AH
authors: West
authors: Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019. Electronic address: pcook@ou.edu.
authors: Paul F
authors: PF
authors: Cook
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D041961:Deuterium Exchange Measurement; D007656:Ketoglutaric Acids / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D007652:Oxo-Acid-Lyases / Q000737:chemistry* / Q000378:metabolism; D016963:Thermus thermophilus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D007656:Ketoglutaric Acids; D000105:Acetyl Coenzyme A; C021365:homocitrate synthase; D007652:Oxo-Acid-Lyases