title: Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome.
abstract: The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine phosphorylation motifs (TPMs), in 32 H. pylori strains isolated from subjects with several forms of gastric diseases, was also explored. High resolution sequence analysis of the above described genes was performed. The nucleotide sequences obtained were translated into amino acids using MEGA (version 4.0) software for EPIYA. An MJ-Network was constructed for obtaining TPM haplotypes by using NETWORK (version 4.5) software. The findings of the study suggest that Indian H. pylori strains share a common ancestry with Europeans. No specific association of haplotypes with the outcome of disease was revealed through additional network analysis of TPMs.
authors: Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, Andhra Pradesh, India.
authors: Santosh K
authors: SK
authors: Tiwari
authors: Vishwas
authors: V
authors: Sharma
authors: Varun Kumar
authors: VK
authors: Sharma
authors: Manoj
authors: M
authors: Gopi
authors: R
authors: R
authors: Saikant
authors: Amrita
authors: A
authors: Nandan
authors: Avinash
authors: A
authors: Bardia
authors: Sivaram
authors: S
authors: Gunisetty
authors: Prasanth
authors: P
authors: Katikala
authors: Md Aejaz
authors: MA
authors: Habeeb
authors: Aleem A
authors: AA
authors: Khan
authors: C M
authors: CM
authors: Habibullah