title: Identification of CEACAM6 as an intermediate filament-associated protein expressed in Sertoli cells of rat testis.
abstract: Ceacam6 (carcinoembryonic antigen-related cell adhesion molecule 6 gene) has recently been isolated by differential display followed by RT-PCR and DNA sequence analyses. Ceacam6 is a member of an immunoglobulin superfamily and encodes a protein of 266 amino acid residues possessing one immunoglobulin (Ig)-like domain. RT-PCR analysis showed that Ceacam6 was dominantly expressed in rat testis and its expression level prominently increased after 6 wk of postnatal development in testis. Immunohistochemical analyses using the anti-CEACAM6 antibody revealed that CEACAM6 colocalized with intermediate filaments (vimentin) in Sertoli cells and interstitial cells. The association between CEACAM6 and vimentin was observed throughout postnatal development in rat testis. Transfection experiments performed in COS-7 cells suggested that overexpression of CEACAM6 brought about aggregation of vimentin filament around nuclei with which CEACAM6 colocalized and that the N-terminus region of CEACAM6, including the Ig-like domain, seemed to be required for association with vimentin filaments. Interaction between CEACAM6 and vimentin in rat testis and transfected COS-7 cells was confirmed by immunoprecipitation. Our observations strongly suggested that CEACAM6 might be a novel intermediate filament-associated protein involved in regulation of vimentin architecture in Sertoli cells.
authors: Laboratory of Zoology, Graduate School of Agriculture, Kyushu University, Fukuoka, Japan.
authors: Hitoshi
authors: H
authors: Kurio
authors: Hiroshi
authors: H
authors: Hatsuda
authors: Emi
authors: E
authors: Murayama
authors: Takane
authors: T
authors: Kaneko
authors: Hiroshi
authors: H
authors: Iida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D019556:COS Cells; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D007382:Intermediate Filaments / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C570771:CEACAM6 protein, rat; D015815:Cell Adhesion Molecules; D014746:Vimentin