title: mRNA expression profiling reveals conserved and non-conserved miR-140 targets.
abstract: microRNAs are non-coding RNAs that regulate gene expression. A significant proportion of microRNAs is perfectly conserved across the vertebrate clade, including miR-140, which is specifically expressed in cartilage. Although it has been computationally predicted that a large majority of microRNA targets are conserved, experimental evidence for this hypothesis remains scarce. In this work we use mRNA expression profiles obtained after manipulation of miR-140 activity levels in human and chicken primary chondrocytes to explore the extent of miR-140 target conservation. Our data suggest that miR-140 has a large number of targets conserved between human and chicken and we validate one of these, BMP2. However, we also found a significant number of non-conserved targets in the two species. In addition, we found that a commercially available scrambled siRNA, which is regularly used as a negative control, regulate the accumulation of many genes.
authors: School of Biological Sciences, University of East Anglia, Norwich, UK.
authors: Francisco Esteban
authors: FE
authors: Nicolas
authors: Helio
authors: H
authors: Pais
authors: Frank
authors: F
authors: Schwach
authors: Morten
authors: M
authors: Lindow
authors: Sakari
authors: S
authors: Kauppinen
authors: Vincent
authors: V
authors: Moulton
authors: Tamas
authors: T
authors: Dalmay
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D017124:Conserved Sequence; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D016415:Sequence Alignment
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering