title: Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria.
abstract: A new commercial low-density microarray which identifies common extended-spectrum β-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.
authors: Laboratoire de Bactériologie, Cliniques Universitaires UCL de Mont-Godinne, Av Dr Therasse 1, B-5530 Yvoir, Belgium. pierre.bogaerts@uclouvain.be
authors: Pierre
authors: P
authors: Bogaerts
authors: Andrea M
authors: AM
authors: Hujer
authors: Thierry
authors: T
authors: Naas
authors: Roberta Rezende
authors: RR
authors: de Castro
authors: Andrea
authors: A
authors: Endimiani
authors: Patrice
authors: P
authors: Nordmann
authors: Youri
authors: Y
authors: Glupczynski
authors: Robert A
authors: RA
authors: Bonomo
mesh_terms: D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology*
chemical_list: D047090:beta-Lactams; D001618:beta-Lactamases