title: [Expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells].
abstract: This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells (MSC). Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine (IBMX) and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was detected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA-DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.
authors: Institute of Hematological Diseases, Lanzhou Military Area Lamzhou General Hospital, Lanzhou 730050, Gansu Province, China.
authors: Bing
authors: B
authors: Wu
authors: Yan-Chun
authors: YC
authors: Wang
authors: Yan
authors: Y
authors: Wei
authors: Ya-Li
authors: YL
authors: Zhou
authors: Ling
authors: L
authors: Zhang
authors: Qian
authors: Q
authors: Zhang
authors: Cun-Bang
authors: CB
authors: Wang
authors: Hai
authors: H
authors: Bai
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism* / Q000528:radiation effects; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D015870:Gene Expression; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000528:radiation effects; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1