title: Physicochemical characterization and antioxidant activity of melanin from a novel strain of Aspergillus bridgeri ICTF-201.
abstract: OBJECTIVE
  The aim of the study is to isolate and characterize a melanin pigment from a new strain of Aspergillus bridgeri isolated from rhizosphere soil of Eucalyptus tree and to investigate its antioxidant activity.
  
  
  RESULTS
  The extracellular pigment was alkali soluble, acid-resistant and insoluble in organic solvents and water. The pigment was precipitated on treatment with FeCl(3), ammoniacal AgNO(3) and potassium ferricyanide and was bleached in the presence of oxidants and reductants. It was confirmed as melanin based on the Fourier transform infrared and electron paramagnetic resonance spectroscopy techniques apart from chemical analysis. Inhibition of melanin production by inhibitors like tricyclazole, 6-hydroxyflavanone, 4-hydroxy-7-methoxy-3-phenyl-coumarin, 7-hydroxy-4-phenyl-coumarin and 7-hydroxy-3,4,8-trimethylcoumarin confirmed that melanin produced by A. bridgeri is synthesized by 1,8-dihydroxynaphthalene (DHN)-melanin pathway. The melanin showed good free radical scavenging activity by DPPH method with an EC(50) of 54.12 μg ml(-1).
  
  
  CONCLUSIONS
  The results of the study indicate that the melanin produced by the newly isolated A. bridgeri strain is a member of DHN melanin family and exhibited significant free radical scavenging activity.
  
  
  CONCLUSIONS
  This is the first report on characterization of DHN melanin produced by a novel strain of A. bridgeri and may find potential application as a natural antioxidant in the cosmetic and pharmaceutical industries.
authors: Chemical Biology Laboratory, Indian Institute of Chemical Technology, Hyderabad, India. cgkumar@iict.res.in
authors: C Ganesh
authors: CG
authors: Kumar
authors: P
authors: P
authors: Mongolla
authors: S
authors: S
authors: Pombala
authors: A
authors: A
authors: Kamle
authors: J
authors: J
authors: Joseph
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism*; D001230:Aspergillus / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000187:drug effects; D004578:Electron Spin Resonance Spectroscopy; D008543:Melanins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D009284:Naphthols; D017550:Spectroscopy, Fourier Transform Infrared; D013844:Thiazoles / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: C423455:1,8-dihydroxynaphthalene melanin; D000975:Antioxidants; D008543:Melanins; D009284:Naphthols; D013844:Thiazoles; C041660:1,8-dihydroxynaphthalene; C041191:tricyclazole