title: Effects of granulocyte-colony stimulating factor on chromosome aneuploidy and replication asynchrony in healthy peripheral blood stem cell donors.
abstract: As peripheral blood has surpassed bone marrow as a predominant source of stem cells for transplantation, use of the cytokine granulocyte colony-stimulating factor (G-CSF) to mobilize peripheral blood stem cells (PBSCs) is increasing. Issues regarding potential genotoxic effects of even short-term, low-dose G-CSF treatment for the healthy donors have been raised. To address the question of chromosomal instability, we used FISH to evaluate the peripheral blood lymphocytes of 22 PBSC donors and 22 matched controls at 5 time points over a 12-month period. The specimens obtained were a pre-G-CSF, followed by collections at the time of PBSC harvest (days 5-7) and at 2, 6, and 12 months after donation. Eight additional PBSC donors provided a single sample at 12 months. Nine loci (mapped to chromosomes 7, 8, 9, 17, 21, and 22) were evaluated for aneuploidy, including 3 mapped to chromosome 7 because of the specific relevance of monosomy 7. Replication timing was evaluated for chromosome 15 and 17 loci. No evidence was found of G-CSF-induced chromosomal instability. This work supports the epidemiologic data that have demonstrated no increased risk for hematologic malignancies in G-CSF-primed PBSC donors.
authors: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN, USA.
authors: Betsy
authors: B
authors: Hirsch
authors: Leann
authors: L
authors: Oseth
authors: Meghan
authors: M
authors: Cain
authors: Erin
authors: E
authors: Trader
authors: Shelley
authors: S
authors: Pulkrabek
authors: Bruce
authors: B
authors: Lindgren
authors: Xianghua
authors: X
authors: Luo
authors: Mary
authors: M
authors: Clay
authors: John
authors: J
authors: Miller
authors: Dennis
authors: D
authors: Confer
authors: Daniel
authors: D
authors: Weisdorf
authors: Jeffrey
authors: J
authors: McCullough
mesh_terms: D000782:Aneuploidy; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002877:Chromosomes, Human / Q000187:drug effects* / Q000648:ultrastructure; D002897:Chromosomes, Human, Pair 7; D004261:DNA Replication / Q000187:drug effects*; D005500:Follow-Up Studies; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology / Q000633:toxicity*; D019650:Hematopoietic Stem Cell Mobilization / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D036102:Peripheral Blood Stem Cell Transplantation; D011446:Prospective Studies; D012107:Research Design; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D016179:Granulocyte Colony-Stimulating Factor