title: Analysis of polymorphism and transcription of the effector gene Avr1b in Phytophthora sojae isolates from China virulent to Rps1b.
abstract: The effector gene Avr1b-1 of Phytophthora sojae determines the efficacy of the resistance gene Rps1b in soybean. The sequences of the Avr1b-1 locus in 34 Chinese isolates of P. sojae were obtained and analysed by polymerase chain reaction (PCR) and inverse PCR. Four different alleles and a complete deletion mutation of the Avr1b-1 gene were identified. Molecular analysis of the deletion breakpoints in the Avr1b-1 locus revealed that an 8-kb DNA sequence containing Avr1b-1 was deleted and a 12.7-kb DNA sequence was inserted at the same locus. A truncated transposase gene was found and five transposable elements were predicted in the inserted sequence, suggesting that the deletion of Avr1b-1 might be attributed to transposon movement. The transcription of Avr1b-1 was analysed in virulent isolates containing four alleles of Avr1b-1 by real-time reverse transcription-PCR. In all virulent isolates, only those isolates containing the second allele transcripted Avr1b-1.
authors: College of Plant Protection, Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing 210095, China.
authors: Linkai
authors: L
authors: Cui
authors: Weixiao
authors: W
authors: Yin
authors: Suomeng
authors: S
authors: Dong
authors: Yuanchao
authors: Y
authors: Wang
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D002681:China; D019295:Computational Biology; D017353:Gene Deletion; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics*; D016678:Genome / Q000235:genetics; D008969:Molecular Sequence Data; D010838:Phytophthora / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology; D011110:Polymorphism, Genetic; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
chemical_list: D011506:Proteins