title: Functional characterisation of Lp_2714, an EAL-domain protein from Lactobacillus plantarum.
abstract: Bioinformatic analysis of lp_2714 from Lactobacillus plantarum WCFS1 demonstrates that it encodes an EAL-domain protein associated with a membrane targeting signal-sequence. Comparison of the predicted primary amino-acid sequence of Lp_2714 shows that it lacks critical catalytic residues and heterologous expression has determined that it does not encode a functional phosphodiesterase. We designate Lp_2714 as a class-3 EAL domain protein probably involved in regulating polysaccharide synthesis on the cell surface the cell.
authors: School of Biosciences, Cardiff University, Cardiff CF10 3US, United Kingdom.
authors: Richard
authors: R
authors: Brown
authors: Julian R
authors: JR
authors: Marchesi
authors: Andrew P
authors: AP
authors: Morby
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016680:Genome, Bacterial; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011135:Polysaccharides, Bacterial; D021382:Protein Sorting Signals