title: Molecular typing of adenovirus circulating in a Colombian paediatric population with acute respiratory infection.
abstract: Human adenoviruses (HAdV) cause a broad spectrum of diseases including acute respiratory infection (ARI), and are responsible for 5% of cases requiring hospitalization in children aged <5 years in Colombia; however, little is known about the circulating types, partly due to the lack of reliable typing tests. In order to evaluate a VA gene PCR-sequencing approach for identification of HAdV circulating types in a Colombian population, 52 nasopharyngeal aspirates/swabs from children with ARI were processed. After a BLAST analysis, matches with species B (41/48, 85·42%), C (6/48, 12·5%), and D (1/48, 2·08%) were found; and at the type level, type 3 (22/48, 45·83%) was the most frequent. This initial effort to expand our knowledge about the molecular epidemiology of HAdV circulating in Bogota, Colombia, showed that HAdV-B was the predominant circulating species in the study period and reports, for the first time in Colombia, the presence of HAdV-D in a respiratory sample.
authors: Laboratorio de Diagnóstico Molecular y Bioinformática, Universidad de los Andes, Bogotá, Colombia.
authors: L J
authors: LJ
authors: Rojas
authors: C A
authors: CA
authors: Jaramillo
authors: M F
authors: MF
authors: Mojica
authors: M P
authors: MP
authors: Escalante
authors: P
authors: P
authors: Delgado
mesh_terms: D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002675:Child, Preschool; D003105:Colombia / Q000453:epidemiology; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D058889:Molecular Typing; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D004279:DNA, Viral