title: An RNA G-quadruplex in the 3' UTR of the proto-oncogene PIM1 represses translation.
abstract: We have identified a conserved G-quadruplex forming sequence in the 3' untranslated region (UTR) of the proto-oncogene PIM1. Circular dichroism and thermal denaturation studies revealed that the PIM1 mRNA guanine-rich sequence forms a highly stable intramolecular G-quadruplex structure. Reporter gene assay demonstrated that the PIM1 RNA G-quadruplex represses translation. It is the first experimental evidence of an RNA G-quadruplex structure located in the 3' UTR that may act as posttranscriptional regulator.
authors: Institut für Molekulare Biowissenschaften, Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt/Main, Germany.
authors: Amit
authors: A
authors: Arora
authors: Beatrix
authors: B
authors: Suess
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D045744:Cell Line, Tumor; D002942:Circular Dichroism; D005656:Fungal Proteins; D054856:G-Quadruplexes; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D000090063:Proto-Oncogene Mas; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D005656:Fungal Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D020928:Mitogen-Activated Protein Kinases; C432694:Pim1 protein, Pichia pastoris