title: Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.
abstract: Detailed studies of host/parasite interactions are currently limited because in situ gene sequencing or monitoring of parasite gene expression is so far limited to genes presenting a high loci copy number in the Schistosome genome or a high level of expression. Indeed, how to investigate the host parasite molecular interplay when parasites are not directly accessible in vivo? Here we describe a method to circumvent this problem and to analyze DNA and RNA of Schistosoma mansoni during the interaction with its intermediate snail host Biomphalaria glabrata. We propose a technique for improved DNA and RNA extraction from the intra-molluscan stage of the parasite recovered after fixation of infected snails in Raillet-Henry solution. The extractions can be used for genetic analysis, transcription studies and microsatellite genotyping.
authors: CNRS, UMR 5244, Ecologie et Evolution des Interactions, Perpignan F-66860, France.
authors: Jean-François
authors: JF
authors: Allienne
authors: André
authors: A
authors: Théron
authors: Benjamin
authors: B
authors: Gourbal
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000469:parasitology*; D006224:Cricetinae; D017931:DNA Primers; D018509:DNA, Helminth / Q000302:isolation & purification*; D004199:Disease Vectors; D008099:Liver / Q000469:parasitology; D008647:Mesocricetus; D018895:Microsatellite Repeats; D033661:Oocysts / Q000502:physiology; D018510:RNA, Helminth / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012550:Schistosoma mansoni / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth; D018510:RNA, Helminth