title: Osteoblast growth and bone-healing response to three-dimensional poly(ε-caprolactone fumarate) scaffolds.
abstract: Poly(ε-caprolactone fumarate) (PCLF) scaffold formulations were assessed as a delivery system for recombinant human bone morphogenetic protein (rhBMP-2) for bone tissue engineering. The formulations included PCLF with combinations of poly(vinyl alcohol) (PVA) and hydroxyapatite (HA). The assessments included in vitro and in vivo assays. In vitro assays validated cell attachment using a pre-osteoblast cell line (MC3T3-E1). Additionally, in vitro release profiles of rhBMP-2 from PCLF scaffolds were determined up to 21 days. The data suggested that PCLF incorporated with PVA and HA accelerated rhBMP-2 release and that the released protein was bioactive. For the in vivo study, a critical-sized defect (CSD) model in rabbit calvaria was used to test PCLF scaffolds. At 6 weeks post-implantation, significantly more bone formation was measured in PCLF scaffolds containing rhBMP-2 than in scaffolds without rhBMP-2. In conclusion, we demonstrated that PCLF delivered biologically active rhBMP-2, promoted bone healing in a CSD and has potential as a bone tissue engineering scaffold.
authors: Department of Biomedical Engineering, Bone Tissue Engineering Center, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
authors: Jinku
authors: J
authors: Kim
authors: Aditi
authors: A
authors: Sharma
authors: Brett
authors: B
authors: Runge
authors: Heather
authors: H
authors: Waters
authors: Bruce
authors: B
authors: Doll
authors: Sean
authors: S
authors: McBride
authors: Pedro
authors: P
authors: Alvarez
authors: Mahrokh
authors: M
authors: Dadsetan
authors: Michael J
authors: MJ
authors: Yaszemski
authors: Jeffrey O
authors: JO
authors: Hollinger
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D017102:Fracture Healing; D050723:Fractures, Bone / Q000473:pathology / Q000628:therapy*; D005650:Fumarates / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism* / Q000473:pathology; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D005650:Fumarates; D011091:Polyesters; D011994:Recombinant Proteins; C506476:poly(caprolactone fumarate)