title: Immunoinformatics prediction of linear epitopes from Taenia solium TSOL18.
abstract: Cysticercosis is a public health problem in several developing countries. The oncosphere protein TSOL18 is the most immunogenic and protective antigen ever reported against porcine cysticercosis, although no specific epitope has been identified to account for these properties. Recent evidence suggests that protection might be associated with conformational epitopes. Linear epitopes from TSOL18 were computationally predicted and evaluated for immunogenicity and protection against porcine cysticercosis. A synthetic peptide was designed based on predicted linear B cell and T cell epitopes that are exposed on the surface of the theoretically modeled structure of TSOL18. Three surface epitopes from TSOL18 were predicted as immunogenic. A peptide comprising a linear arrangement of these epitopes was chemically synthesized. The capacity of the synthetic peptide to protect pigs against an oral challenge with Taenia solium proglottids was tested in a vaccine trial. The synthetic peptide was able to produce IgG antibodies in pigs and was associated to a reduction of the number of cysts, although was not able to provide complete protection, defined as the complete absence of cysts in necropsy. This study demonstrated that B cell and T cell predicted epitopes from TSOL18 were not able to completely protect pigs against an oral challenge with Taenia solium proglottids. Therefore, other linear epitopes or eventually conformational epitopes may be responsible for the protection conferred by TSOL18.
authors: Mirko
authors: M
authors: Zimic
authors: Andrés Hazaet
authors: AH
authors: Gutiérrez
authors: Robert Hugh
authors: RH
authors: Gilman
authors: César
authors: C
authors: López
authors: Miguel
authors: M
authors: Quiliano
authors: Wilfredo
authors: W
authors: Evangelista
authors: Armando
authors: A
authors: Gonzales
authors: Héctor Hugo
authors: HH
authors: García
authors: Patricia
authors: P
authors: Sheen