title: Isolation of Succinivibrionaceae implicated in low methane emissions from Tammar wallabies.
abstract: The Tammar wallaby (Macropus eugenii) harbors unique gut bacteria and produces only one-fifth the amount of methane produced by ruminants per unit of digestible energy intake. We have isolated a dominant bacterial species (WG-1) from the wallaby microbiota affiliated with the family Succinivibrionaceae and implicated in lower methane emissions from starch-containing diets. This was achieved by using a partial reconstruction of the bacterium's metabolism from binned metagenomic data (nitrogen and carbohydrate utilization pathways and antibiotic resistance) to devise cultivation-based strategies that produced axenic WG-1 cultures. Pure-culture studies confirm that the bacterium is capnophilic and produces succinate, further explaining a microbiological basis for lower methane emissions from macropodids. This knowledge also provides new strategic targets for redirecting fermentation and reducing methane production in livestock.
authors: CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia 4067, Australia.
authors: P B
authors: PB
authors: Pope
authors: W
authors: W
authors: Smith
authors: S E
authors: SE
authors: Denman
authors: S G
authors: SG
authors: Tringe
authors: K
authors: K
authors: Barry
authors: P
authors: P
authors: Hugenholtz
authors: C S
authors: CS
authors: McSweeney
authors: A C
authors: AC
authors: McHardy
authors: M
authors: M
authors: Morrison
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D004064:Digestive System / Q000382:microbiology*; D005260:Female; D005285:Fermentation; D016680:Genome, Bacterial; D007614:Macropodidae / Q000382:microbiology*; D054892:Metagenome; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D013213:Starch / Q000378:metabolism; D019802:Succinic Acid / Q000378:metabolism*; D044063:Succinivibrionaceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D013213:Starch; D019802:Succinic Acid; D008697:Methane