title: High-resolution extracellular stimulation of dispersed hippocampal culture with high-density CMOS multielectrode array based on non-Faradaic electrodes.
abstract: We introduce a method to electrically stimulate individual neurons at single-cell resolution in arbitrary spatiotemporal patterns with precise control over stimulation thresholds. By exploiting a custom microelectronic chip, up to 65,000 non-Faradaic electrodes can be uniquely addressed with electrode density exceeding 6500 electrodes mm(-2). We demonstrate extracellular stimulation of dispersed primary hippocampal neuronal cultures using the chip at single-cell resolution.
authors: Bioelectronic Systems Lab, Department of Electrical Engineering, Columbia University, 500 West 120th Street 1300, New York, NY, USA. leina@ee.columbia.edu
authors: N
authors: N
authors: Lei
authors: S
authors: S
authors: Ramakrishnan
authors: P
authors: P
authors: Shi
authors: J S
authors: JS
authors: Orcutt
authors: R
authors: R
authors: Yuste
authors: L C
authors: LC
authors: Kam
authors: K L
authors: KL
authors: Shepard
mesh_terms: D000465:Algorithms; D000818:Animals; D016477:Artifacts; D020013:Calcium Signaling / Q000502:physiology; D002138:Calibration; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D005110:Extracellular Space / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008839:Microelectrodes; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012666:Semiconductors