title: Development of 30 novel polymorphic Expressed Sequence Tags (EST)-derived microsatellite markers for the Miiuy Croaker, Miichthys miiuy.
abstract: Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were from 0.1024 to 0.7917 and from 0.2732 to 0.8845, respectively. Nine loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium was significant between 22 pairs of loci. These polymorphic microsatellite loci will be useful for genetic diversity analysis and molecule-assisted breeding for M. miiuy.
authors: Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China; E-Mails: tianjunxu@163.com (T.X.); ssundianqiaod@163.com (D.S.); yuenasun@163.com (Y.S.).
authors: Tianjun
authors: T
authors: Xu
authors: Dianqiao
authors: D
authors: Sun
authors: Yuena
authors: Y
authors: Sun
authors: Rixin
authors: R
authors: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D017931:DNA Primers / Q000378:metabolism; D020224:Expressed Sequence Tags; D015723:Gene Library; D056426:Genetic Loci; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers