title: C. trachomatis in female reproductive tract infections and RFLP-based genotyping: a 16-year study from a tertiary care hospital.
abstract: Presence of Chlamydia trachomatis in endocervix was determined in 2466 women attending a tertiary care hospital in New Delhi, India over a period of 16 years, using a monoclonal-based direct immunofluorescence assay, tissue culture isolation, and a conventional PCR assay. Chlamydia antigen could be detected in 391 out of 2466 (15.85%) of patients studied; in 27.27% women with PID, 16.74% women with cervicitis, 16.03% women with infertility, and 12.06% women with adverse pregnancy outcomes, respectively. There was a statistically significant decreasing trend in Chlamydia antigen positivity between the years 1994-1999 and 2000-2004; the apparent decline in antigen positivity between the years 2000-2004 and 2005-2010 was not statistically significant. Antigen detection assay detected equal number of positives as the PCR assay; tissue culture isolation demonstrated lower positivity. In a few representative specimens from cervicitis patients, genotyping was done using RFLP pattern analysis of C. trachomatis MOMP gene amplified by PCR assay, all of these belonged to Chlamydia trachomatis serovar E.
authors: Department of Ocular Microbiology, Dr RP Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India. gita.satpathy@gmail.com
authors: Satpathy
authors: S
authors: Gita
authors: Mittal
authors: M
authors: Suneeta
authors: Sharma
authors: S
authors: Anjana
authors: Nayak
authors: N
authors: Niranjan
authors: Mohanty
authors: M
authors: Sujata
authors: R M
authors: RM
authors: Pandey
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000235:genetics; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000235:genetics*; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology*; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008137:Longitudinal Studies; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D018272:Porins / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis