title: Validation study on avermectine residues in foodstuffs.
abstract: Avermectines are antiparasitic agents widely used as veterinary drugs for food producing animals. The European Community, due to their side effects, limited the use of these molecules establishing maximum residue limits (MRLs) in some foods. A validated qualitative and quantitative high performance liquid chromatography method with fluorescence detection (HPLC-FL) is presented for the simultaneous determination of ivermectin (IVM), abemectin (ABA), moxidectin (MOX), eprinomectin (EPR), doramectin (DOR) and emamectin (EMA) in foodstuffs (muscle, eggs and milk). Samples were extracted with acetonitrile, purified with liquid-liquid extraction (LLE), and analysed by HLPC-FL previous derivatization with trifluoroacetic anhydride (TFAA) in presence of 1-methyl-imidazole (MI) and acetic acid. To date, the presented method is the first validated for the matrix eggs, and in accordance with the requirements set by Commission Decision 2002/657/EC. Recoveries of the methods, calculated spiking the samples in the range 5.0-100.0 μg kg(-1), were 64-83% for muscle, 65-89% for milk and 63-84% for eggs. The precision (CV) ranged between 9.2 and 17.1% for muscle, 9.9 and 16.6% for milk and from 9.4 to 17.4% for eggs. Linearity for the six analytes was calculated from 5.0 to 200.0 μg kg(-1). The main advantages of the presented method are its rapidity, the specificity, the good precision and recovery that make it very suitable to the detection and determination of avermectines.
authors: Istituto Zooprofilattico Sperimentale Regioni Lazio e Toscana, Rome, Italy. luigi.giannetti@izslt.it
authors: L
authors: L
authors: Giannetti
authors: A
authors: A
authors: Giorgi
authors: F
authors: F
authors: Necci
authors: G
authors: G
authors: Ferretti
authors: F
authors: F
authors: Buiarelli
authors: B
authors: B
authors: Neri
mesh_terms: D000089:Acetic Anhydrides; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D000977:Antiparasitic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis; D005463:Fluoroacetates; D005504:Food Analysis / Q000379:methods*; D008892:Milk / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D014269:Trifluoroacetic Acid / Q000737:chemistry
chemical_list: D000089:Acetic Anhydrides; D000097:Acetonitriles; D000977:Antiparasitic Agents; D005463:Fluoroacetates; C017958:trifluoroacetic anhydride; D014269:Trifluoroacetic Acid; C032159:acetonitrile