title: A small cytoplasmic region adjacent to the fourth transmembrane segment of the α7 nicotinic receptor is essential for its biogenesis.
abstract: Deletion of a small cytoplasmic fragment close to the fourth transmembrane segment of the nicotinic α7 receptor (Glu437 to Arg447) abolished membrane expression. Different single mutants showed moderate to strong decreases in expression whereas the latter was totally abolished upon proline substitutions. We hypothesize that preservation of an α-helix formed by the fourth transmembrane segment and the adjacent cytoplasmic region is essential for membrane receptor expression. Moreover, in selected mutants with low or null membrane expression, a significant proportion of mature receptors was present inside the cell. Hence, elements in this cytoplasmic fragment might influence receptor transport to the membrane.
authors: Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, Sant Joan d'Alacant, Alicante, Spain. manuel.criado@umh.es
authors: Manuel
authors: M
authors: Criado
authors: José
authors: J
authors: Mulet
authors: Susana
authors: S
authors: Gerber
authors: Salvador
authors: S
authors: Sala
authors: Francisco
authors: F
authors: Sala
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle; D003593:Cytoplasm / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis; D009154:Mutation; D017433:Protein Structure, Secondary; D011978:Receptors, Nicotinic / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D064569:alpha7 Nicotinic Acetylcholine Receptor
chemical_list: D008565:Membrane Proteins; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor