title: Synthesis, characterization and antibacterial activity against Gram positive and Gram negative bacteria of biomimetically coated silver nanoparticles.
abstract: In the present work, we describe a simple procedure to produce biomimetically coated silver nanoparticles (Ag NPs), based on the postfunctionalization and purification of colloidal silver stabilized by citrate. Two biological capping agents have been used (cysteine Cys and glutathione GSH). The composition of the capped colloids has been ascertained by different techniques and antibacterial tests on GSH-capped Ag NPs have been conducted under physiological conditions, obtaining values of Minimum Inhibitory Concentration (MIC) of 180 and 15 μg/mL for Staphylococcus aureus and Escherichia coli, respectively. The antibacterial activity of these GSH capped NPs can be ascribed to the direct action of metallic silver NPs, rather than to the bulk release of Ag(+).
authors: Dipartimento di Chimica, Sezione di Chimica Generale, Università di Pavia, viale Taramelli, 12-27100 Pavia, Italy.
authors: Elvio
authors: E
authors: Amato
authors: Yuri A
authors: YA
authors: Diaz-Fernandez
authors: Angelo
authors: A
authors: Taglietti
authors: Piersandro
authors: P
authors: Pallavicini
authors: Luca
authors: L
authors: Pasotti
authors: Lucia
authors: L
authors: Cucca
authors: Chiara
authors: C
authors: Milanese
authors: Pietro
authors: P
authors: Grisoli
authors: Cesare
authors: C
authors: Dacarro
authors: Jose M
authors: JM
authors: Fernandez-Hechavarria
authors: Vittorio
authors: V
authors: Necchi
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003102:Colloids; D012834:Silver