title: Synthesis and biological evaluation of derivatives of 4-deoxypodophyllotoxin as antitumor agents.
abstract: In an attempt to generate compounds with superior bioactivity and reduced toxicity, a series of derivatives of deoxypodophyllotoxin were synthesized by reacting 4'-demethyl-4-deoxypodophyllotoxin with substituted piperazines or their amino acid amides. The cytotoxic activity of these compounds against three human cancer cell lines was evaluated. We found that p-nitrophenylpiperazine substitution (Compound 8b) led to an increase in the potency of the compound. Compound 8b exhibited the most potent cytotoxicity against A-549, HeLa and SiHa cells (IC(50) values were 0.102, 0.180 and 0.0195 μM, respectively). In addition, flow cytometric analysis showed that 8b induced cell cycle arrest in the G1 phase accompanied by apoptosis in A-549 cells.
authors: College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors: Yan
authors: Y
authors: Jin
authors: Jie
authors: J
authors: Liu
authors: Wen-Ting
authors: WT
authors: Huang
authors: Shi-Wu
authors: SW
authors: Chen
authors: Ling
authors: L
authors: Hui
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D013055:Spectrophotometry, Infrared
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; C014451:deoxypodophyllotoxin; D011034:Podophyllotoxin