title: Seroprevalence of antibodies against Anisakis simplex larvae among health-examined residents in three hospitals of southern parts of Korea.
abstract: The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.
authors: Department of Parasitology and Genetics, Kosin University College of Medicine, Busan 602-702, Korea.
authors: Jung
authors: J
authors: Kim
authors: Jin Ok
authors: JO
authors: Jo
authors: Seon Hee
authors: SH
authors: Choi
authors: Min Kyoung
authors: MK
authors: Cho
authors: Hak Sun
authors: HS
authors: Yu
authors: Hee Jae
authors: HJ
authors: Cha
authors: Meesun
authors: M
authors: Ock
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017129:Anisakiasis / Q000453:epidemiology*; D017163:Anisakis / Q000276:immunology*; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000737:chemistry; D015153:Blotting, Western; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006761:Hospitals; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007723:Korea / Q000453:epidemiology; D007814:Larva / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D016036:Seroepidemiologic Studies; D017678:Sex Distribution; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007073:Immunoglobulin E