title: Generation and characterization of neurogenin1-GFP transgenic medaka with potential for rapid developmental neurotoxicity screening.
abstract: Fish models such as zebrafish and medaka are increasingly used as alternatives to rodents in developmental and toxicological studies. These developmental and toxicological studies can be facilitated by the use of transgenic reporters that permit the real-time, noninvasive observation of the fish. Here we report the construction and characterization of transgenic medaka lines expressing green fluorescent protein (GFP) under the control of the zebrafish neurogenin 1 (ngn1) gene promoter. Neurogenin (ngn1) is a helix-loop-helix transcription factor expressed in proliferating neuronal progenitor cells early in neuronal differentiation and plays a crucial role in directing neurogenesis. GFP expression was detected from 24 h post-fertilization until hatching, in a spatial pattern consistent with the previously reported zebrafish ngn1 expression. Temporal expression of the transgene parallels the expression profile of the endogenous medaka ngn1 transcript. Further, we demonstrate that embryos from the transgenic line permit the non-destructive, real-time screening of ngn1 promoter-directed GFP expression in a 96-well format, enabling higher throughput studies of developmental neurotoxicants. This strain has been deposited with and maintained by the National BioResource Project and is available on request (http://www.shigen.nig.ac.jp/medaka/strainDetailAction.do?quickSearch=true&strainId=5660).
authors: Integrated Systems Toxicology and Toxicity Assessment Divisions, National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA.
authors: Chun-Yang
authors: CY
authors: Fan
authors: Steven O
authors: SO
authors: Simmons
authors: Sheran H W
authors: SH
authors: Law
authors: Karl
authors: K
authors: Jensen
authors: John
authors: J
authors: Cowden
authors: David
authors: D
authors: Hinton
authors: Stephanie
authors: S
authors: Padilla
authors: Ram
authors: R
authors: Ramabhadran
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009420:Nervous System / Q000187:drug effects* / Q000378:metabolism; D009990:Oryzias / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D029941:Fish Proteins; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C486337:neurog1 protein, zebrafish