title: Evaluation of pathogenesis caused in cattle and guinea pig by a Mycobacterium bovis strain isolated from wild boar.
abstract: BACKGROUND
  In many regions of the world, wild mammals act as reservoir of Mycobacterium bovis, a situation that prevents the eradication of bovine tuberculosis. In order to observe whether a strain isolated from a wild boar, previously tested as highly virulent in a mice model, is also virulent in cattle, we performed cattle experimental inoculation with this strain
  
  
  RESULTS
  Groups of Friesian calves were either infected with the wild boar strain M. bovis 04-303 or with the bovine strain NCTC10772 as a control. We found that antigen-specific IFN-γ release in whole blood samples occurred earlier in animals infected with M. bovis 04-303. Both M. bovis strains resulted in a positive skin test, with animals infected with the wild boar isolate showing a stronger response. These results and the presence of more severe organ lesions, with granuloma and pneumonic areas in cattle demonstrate that the wild boar isolate is more virulent than the NCTC10772 strain. Additionally, we tested the infectivity of the M. bovis strains in guinea pigs and found that M. bovis 04-303 had the highest pathogenicity.
  
  
  CONCLUSIONS
  M. bovis strains isolated from wild boars may be pathogenic for cattle, producing TB lesions.
authors: Institute of Biotechnology, Hurlingham, Argentina.
authors: Virginia
authors: V
authors: Meikle
authors: María V
authors: MV
authors: Bianco
authors: Federico C
authors: FC
authors: Blanco
authors: Andrea
authors: A
authors: Gioffré
authors: Sergio
authors: S
authors: Garbaccio
authors: Lucas
authors: L
authors: Vagnoni
authors: Julio
authors: J
authors: Di Rienzo
authors: Ana
authors: A
authors: Canal
authors: Fabiana
authors: F
authors: Bigi
authors: Angel
authors: A
authors: Cataldi
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001681:Biological Assay / Q000662:veterinary; D002417:Cattle; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004197:Disease Reservoirs / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D006168:Guinea Pigs; D006651:Histocytochemistry / Q000662:veterinary; D007371:Interferon-gamma / Q000097:blood; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000662:veterinary; D034421:Sus scrofa / Q000382:microbiology*; D014380:Tuberculosis, Bovine / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology* / Q000635:transmission; D014774:Virulence
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D007371:Interferon-gamma