title: Determination of 5-hydroxytryptamine in serum by electrochemiluminescence detection with the aid of capillary electrophoresis.
abstract: A rapid, sensitive and simple electrochemiluminescence method for the determination of 5-hydroxytryptamine (5-HT) using capillary electrophoresis was proposed. The experimental parameters, including the detection potential, the concentration of Ru(bpy)(3)(2+), the concentration and pH of phosphate buffer for separation and detection, the injection voltage and time and the separation voltage on the determination of 5-HT, were optimized. Under the optimized conditions, the linear concentration range for 5-HT was 3.5 × 10(-9) -5.1 × 10(-3) mol/L, with a detection limit of 5 × 10(-10) mol/L. The relative standard deviations (RSDs) of the ECL intensity and the migration times for six continuous injections of 1.0 µmol/L 5-HT were 2.48% and 1.3%, respectively. The method was successfully applied to 5-HT assay in samples of human serum in 5 min and the extraction recoveries with spiked serum samples were over 94.4%.
authors: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, People's Republic of China.
authors: Yuefang
authors: Y
authors: Hu
authors: Wei
authors: W
authors: Xu
authors: Jianping
authors: J
authors: Li
authors: Lanjuan
authors: L
authors: Li
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D012428:Ruthenium / Q000737:chemistry; D012701:Serotonin / Q000097:blood*
chemical_list: D012701:Serotonin; D012428:Ruthenium