title: Simplified off-gas analyses in animal cell cultures for process monitoring and control purposes.
abstract: Batch-to-batch reproducibility of animal cell cultures can significantly be enhanced using process control procedures. Most informative signals for advanced process control can be derived from the volume fractions of oxygen and carbon dioxide in the vent line of the reactors. Here we employed simple low-cost sensors, previously not considered for off-gas analysis at a laboratory-scale cell cultures, and compared them with a simultaneously used quadrupole mass spectrometer, i.e., the standard equipment. A decisive advantage is that the sensors did not need any calibration and are easy to use. We show that monitoring and advanced control of cell cultures can significantly be simplified using the devices tested here and that the same batch-to-batch reproducibility can be obtained with much less effort than before.
authors: Institute of Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany. mathias.aehle@biochemtech.uni-halle.de
authors: Mathias
authors: M
authors: Aehle
authors: Artur
authors: A
authors: Kuprijanov
authors: Sebastian
authors: S
authors: Schaepe
authors: Rimvydas
authors: R
authors: Simutis
authors: Andreas
authors: A
authors: Lübbert
mesh_terms: D000818:Animals; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D016466:CHO Cells; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D005740:Gases / Q000737:chemistry*; D010101:Oxygen Consumption
chemical_list: D005740:Gases; D002245:Carbon Dioxide