title: Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional Chinese medicine preparation.
abstract: A novel method combining high performance liquid chromatography (HPLC) fingerprint and simultaneous quantitative analysis of multiple active components was developed and validated for quality evaluation of one type of traditional Chinese medicine preparations: Shuang-huang-lian (SHL) oral liquid formulation. For fingerprint analysis, 45 peaks were selected as the common peaks to evaluate the similarities among several different SHL oral liquid preparations collected from manufacturers. Additionally, simultaneous quantification of eleven markers, including chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, apigenin, baicalein and wogonin, was performed. Statistical analysis of the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Finally, concentrations of these eleven markers in SHL oral liquid prepared by different manufacturers in China were determined. These results demonstrated that the combination of HPLC chromatographic fingerprint and simultaneous quantification of multi-ingredients offers an efficient and reliable approach for quality evaluation of SHL oral liquid preparations.
authors: Department of Pharmaceutical Analysis, Xuzhou Medical College, Xuzhou Jiangsu 221004, China.
authors: Dong-Zhi
authors: DZ
authors: Yang
authors: Yi-Qiang
authors: YQ
authors: An
authors: Xiang-Lan
authors: XL
authors: Jiang
authors: Dao-Quan
authors: DQ
authors: Tang
authors: Yuan-Yuan
authors: YY
authors: Gao
authors: Hong-Tao
authors: HT
authors: Zhao
authors: Xiao-Wen
authors: XW
authors: Wu
mesh_terms: D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004339:Drug Compounding / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D008516:Medicine, Chinese Traditional / Q000592:standards*; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; C516400:shuang-huang-lian