title: Gastrointestinal digestion and absorption of Pen j 1, a major allergen from Kuruma prawn, Penaeus japonicus.
abstract: Tropomyosin had been identified as a major allergen in shrimp. The digestion and absorption of tropomyosin (Pen j 1) from kuruma prawn were investigated by ex vivo, in vitro, and in vivo techniques in order to elucidate the relationship between the allergenicity of the allergen and its gastrointestinal behavior. Pen j 1 transported the Caco-2 monolayer in a dose-dependent manner, and also enhanced the permeability of lucifer yellow, a marker of paracellular transportation, at high concentrations of the allergen. Studies with everted sacs revealed that Pen j 1 was rapidly degraded to small peptides (MW<3.5 kDa) and amino acids by intestinal proteases and absorbed from enterocytes. Furthermore, Pen j 1 orally administered to rats tended to remain in the stomach rather than in the small intestine, after which the allergen moved to the epithelial cells. These observations suggest that Pen j 1 may be absorbed via the gastric mucosa prior to its digestion in the intestines.
authors: Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Japan.
authors: Ayumi
authors: A
authors: Kunimoto
authors: Miyuki
authors: M
authors: Yokoro
authors: Kaeko
authors: K
authors: Murota
authors: Rintaro
authors: R
authors: Yamanishi
authors: Toshiko
authors: T
authors: Suzuki-Yamamoto
authors: Makiko
authors: M
authors: Suzuki
authors: Chikao
authors: C
authors: Yutani
authors: Shinobu
authors: S
authors: Doi
authors: Miki
authors: M
authors: Hiemori
authors: Hiromi
authors: H
authors: Yamashita
authors: Yoshitaka
authors: Y
authors: Takahashi
authors: Hideaki
authors: H
authors: Tsuji
authors: Masumi
authors: M
authors: Kimoto
mesh_terms: D000042:Absorption; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D018938:Caco-2 Cells; D002460:Cell Line; D002463:Cell Membrane Permeability; D049109:Cell Proliferation; D004063:Digestion / Q000276:immunology*; D041981:Gastrointestinal Tract / Q000276:immunology*; D006801:Humans; D008297:Male; D033561:Penaeidae / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
chemical_list: D000485:Allergens