title: Detection, isolation, and characterization of acidophilic methanotrophs from Sphagnum mosses.
abstract: Sphagnum peatlands are important ecosystems in the methane cycle. Methane-oxidizing bacteria in these ecosystems serve as a methane filter and limit methane emissions. Yet little is known about the diversity and identity of the methanotrophs present in and on Sphagnum mosses of peatlands, and only a few isolates are known. The methanotrophic community in Sphagnum mosses, originating from a Dutch peat bog, was investigated using a pmoA microarray. A high biodiversity of both gamma- and alphaproteobacterial methanotrophs was found. With Sphagnum mosses as the inoculum, alpha- and gammaproteobacterial acidophilic methanotrophs were isolated using established and newly designed media. The 16S rRNA, pmoA, pxmA, and mmoX gene sequences showed that the alphaproteobacterial isolates belonged to the Methylocystis and Methylosinus genera. The Methylosinus species isolated are the first acid-tolerant members of this genus. Of the acidophilic gammaproteobacterial strains isolated, strain M5 was affiliated with the Methylomonas genus, and the other strain, M200, may represent a novel genus, most closely related to the genera Methylosoma and Methylovulum. So far, no acidophilic or acid-tolerant methanotrophs in the Gammaproteobacteria class are known. All strains showed the typical features of either type I or II methanotrophs and are, to the best of our knowledge, the first isolated (acidophilic or acid-tolerant) methanotrophs from Sphagnum mosses.
authors: Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Nijmegen, The Netherlands.
authors: Nardy
authors: N
authors: Kip
authors: Wenjing
authors: W
authors: Ouyang
authors: Julia
authors: J
authors: van Winden
authors: Ashna
authors: A
authors: Raghoebarsing
authors: Laura
authors: L
authors: van Niftrik
authors: Arjan
authors: A
authors: Pol
authors: Yao
authors: Y
authors: Pan
authors: Levente
authors: L
authors: Bodrossy
authors: Elly G
authors: EG
authors: van Donselaar
authors: Gert-Jan
authors: GJ
authors: Reichart
authors: Mike S M
authors: MS
authors: Jetten
authors: Jaap S Sinninghe
authors: JS
authors: Damsté
authors: Huub J M
authors: HJ
authors: Op den Camp
mesh_terms: D000143:Acids / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D017753:Ecosystem; D005798:Genes, Bacterial; D008697:Methane / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D044003:Sphagnopsida / Q000382:microbiology*
chemical_list: D000143:Acids; D003470:Culture Media; D004269:DNA, Bacterial; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D008697:Methane