title: Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis.
abstract: The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
authors: Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Sciences, West Pomeranian University of Technology in Szczecin, Doktora Judyma 24, 71-466 Szczecin, Poland. jolanta.karakulska@zut.edu.pl
authors: J
authors: J
authors: Karakulska
authors: A
authors: A
authors: Pobucewicz
authors: P
authors: P
authors: Nawrotek
authors: M
authors: M
authors: Muszyńska
authors: A J
authors: AJ
authors: Furowicz
authors: D
authors: D
authors: Czernomysy-Furowicz
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015373:Bacterial Typing Techniques; D002417:Cattle; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008414:Mastitis, Bovine / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins