title: T-DNA as a gene tag.
abstract: The characteristics of Agrobacterium-mediated plant transformation are such that the T-DNA can be used as an insertional mutagen and, by extension, a gene tag. An increasing number of mutants have been obtained as a result of T-DNA insertion and the versatility of this experimental system can be exploited further to tag sequences of DNA which control gene expression. While the isolation of specific mutations by T-DNA tagging in the past has been fortuitous, T-DNA-based vectors have the potential to generate transgenic plants with specific, selectable mutations.
authors: Max-Planck-institut für Züchtungsforschung, Carl von Linne Weg 10, D5000 Kö ln 30, Germany.
authors: Walden
authors: W
authors: Richard
authors: Max-Planck-institut für Züchtungsforschung, Carl von Linne Weg 10, D5000 Kö ln 30, Germany.
authors: Hiroaki
authors: H
authors: Hayashi
authors: Max-Planck-institut für Züchtungsforschung, Carl von Linne Weg 10, D5000 Kö ln 30, Germany.
authors: Jeff
authors: J
authors: Schell
mesh_terms: D004269:DNA, Bacterial; D018744:DNA, Plant; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA