title: Recombinant immunotoxin anti-c-Met/PE38KDEL inhibits proliferation and promotes apoptosis of gastric cancer cells.
abstract: BACKGROUND
  Our study aims to evaluate the anti-growth effects of recombinant immunotoxin (IT) anti-c-Met/PE38KDEL on gastric cancer cells, and its mechnisms.
  
  
  METHODS
  Gastric cancer cells were treated with increasing doses of IT and c-Met protein was quantified by Western blotting. Cell proliferation was determined by Cell Counting Kit-8 assay (CCK). [3H]-leucine incorporation assay was used to evaluate IT inhibition of protein synthesis. Cell apoptosis was quantified by flow cytometry. Caspase activities were measured using colorimetric protease assays.
  
  
  RESULTS
  Cell growth and protein synthesis of the gastric cancer cell lines were suppressed by IT in a dose- and time-dependent manner. IT also induced apoptosis in a dose-dependent manner. The apoptosis rates of gastric cancer cell lines MKN-45 and SGC7901 were 19.19% and 27.37%, respectively when treated with 50 ng/ml of IT. There were significant increase of caspase-3 activity at 24 hr of IT treatment (100 ng/ml) (P < 0.01) in these gastric cancer cell lines.
  
  
  CONCLUSIONS
  IT anti-c-Met/PE38KDEL has anti-growth effects on the gastric cancer cell lines in vitro, and it provides an experimental basis for c-Met-targeted therapy towards in vivo testing.
authors: Department of Gastroenterology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, PR China.
authors: Xu
authors: X
authors: Wei
authors: Zhu Xiao
authors: ZX
authors: Juan
authors: Feng Xiao
authors: FX
authors: Min
authors: Cai
authors: C
authors: Nan
authors: Zhang Xiu
authors: ZX
authors: Hua
authors: Feng Zheng
authors: FZ
authors: Qing
authors: Liu
authors: L
authors: Zheng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D005098:Exotoxins / Q000494:pharmacology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology / Q000494:pharmacology; D000922:Immunotoxins / Q000494:pharmacology*; D019859:Proto-Oncogene Proteins c-met / Q000096:biosynthesis / Q000276:immunology; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D005098:Exotoxins; D007128:Immunoglobulin Fragments; D000922:Immunotoxins; D019859:Proto-Oncogene Proteins c-met; D053148:Caspase 3