title: Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus.
abstract: Sp1-like proteins and Kruppel-like factors (KLFs) are highly related zinc-finger proteins that have crucial roles in transcription. One expressed sequence tag (EST, HPA-N-S01-EST0038) from shrimps is homologous to Sp1. This study reports the cloning and characteristics of a KLF from shrimp, Penaeus monodon (PmKLF). The full-length PmKLF cDNA is 1702 bp, encoding a polypeptide of 360 amino acids. Sequence analysis revealed that the sequence of PmKLF is similar to that of KLF11 in humans, mice and zebrafish. RT-PCR analysis indicated that PmKLF mRNA is expressed in all examined tissues. Additionally, immunofluorescence analysis revealed that GFP-KLF fusion protein is located in the nucleus as dots in an insect cell line, Sf9. Localization of PmKLF in the nucleus is also observed in the hemolymph from white spot syndrome virus (WSSV)-infected and WSSV-uninfected Litopenaeus vannamei. Knockdown of the expression of PmKLF transcript in WSSV-infected shrimp resulted in delayed cumulative mortalities, suggesting that PmKLF is important to WSSV infection. Moreover, inhibition of PmKLF expression reduced the copy number of WSSV and ie1 expression, revealing that PmKLF affects WSSV infection via interfering with ie1 expression.
authors: Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, ROC. changlk@ntu.edu.tw
authors: Li-Kwan
authors: LK
authors: Chang
authors: Ping-Han
authors: PH
authors: Huang
authors: Wei-Tao
authors: WT
authors: Shen
authors: Shu-Han
authors: SH
authors: Yang
authors: Wang-Jing
authors: WJ
authors: Liu
authors: Chu-Fang
authors: CF
authors: Lo
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D055785:Gene Knockdown Techniques; D006458:Hemolymph / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007313:Insecta; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D033561:Penaeidae; D012097:Repressor Proteins / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000472:pathogenicity / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; C115117:KLF11 protein, human; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D016329:Sp1 Transcription Factor; D014764:Viral Proteins