title: Osteopontin upregulation in rotavirus-induced murine biliary atresia requires replicating virus but is not necessary for development of biliary atresia.
abstract: Biliary atresia (BA) is a progressive fibro-inflammatory pediatric liver disease in which osteopontin (OPN), a glycoprotein with inflammatory and fibrogenic activity, may play a pathogenic role. The current studies were conducted in a mouse model of rotavirus-induced BA to test the hypotheses that live but not inactivated rotavirus causes antigenemia, upregulation of hepatic OPN expression, and induction of BA and fibrosis; and that OPN is necessary for development of BA. Prolonged or transient antigenemia developed in mice inoculated with live or inactivated virus, respectively, but only live virus upregulated hepatic OPN and caused BA and fibrosis. OPN was expressed in intra- and extrahepatic bile ducts in healthy mice and in mice with BA. OPN-deficient mice, similar to WT mice, developed BA. Together, these data show that live but not inactivated rotavirus causes upregulation of hepatic OPN expression and BA but that OPN is not necessary for development of BA.
authors: Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA. phertel@bcm.edu
authors: Paula M
authors: PM
authors: Hertel
authors: Sue E
authors: SE
authors: Crawford
authors: Milton J
authors: MJ
authors: Finegold
authors: Mary K
authors: MK
authors: Estes
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001656:Biliary Atresia / Q000473:pathology*; D004195:Disease Models, Animal; D006801:Humans; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D053495:Osteopontin / Q000096:biosynthesis*; D012376:Rodent Diseases / Q000473:pathology / Q000821:virology; D012400:Rotavirus Infections / Q000473:pathology*; D015854:Up-Regulation; D014766:Viremia
chemical_list: C506896:Spp1 protein, mouse; D053495:Osteopontin