title: Claudin-4: functional studies beyond the tight junction.
abstract: Claudin-4 is an unusual member of the claudin family; in addition to its role in epithelial tight junction barrier function, it is a receptor for the Clostridium perfringens enterotoxin. We have also found that claudin-4 is regulated in mucosal epithelium M cells, both in increased expression of the protein and in redistribution into endocytosis vesicles. Our ongoing studies are studying the potential for developing ligands specific to claudin-4 for targeted delivery of cargo such as proteins and poly(DL-lactide-co-glycolide) nanoparticles to mucosal M cells. Methods for the study of claudin-4 movement within epithelial cells, and delivery of nanoparticles through targeted binding of claudin-4 are described.
authors: Holly A
authors: HA
authors: Eckelhoefer
authors: Thejani E
authors: TE
authors: Rajapaksa
authors: Jing
authors: J
authors: Wang
authors: Mary
authors: M
authors: Hamer
authors: Nancy C
authors: NC
authors: Appleby
authors: Jun
authors: J
authors: Ling
authors: David D
authors: DD
authors: Lo
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology*; D062506:Claudin-4; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D016503:Drug Delivery Systems / Q000379:methods*; D004768:Enterotoxins / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods; D049452:Green Fluorescent Proteins; D006651:Histocytochemistry / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009092:Mucous Membrane / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D020349:Surface Plasmon Resonance / Q000379:methods; D022161:Transport Vesicles / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D062506:Claudin-4; D017931:DNA Primers; D004768:Enterotoxins; D008024:Ligands; D008565:Membrane Proteins; C035598:enterotoxin, Clostridium; D049452:Green Fluorescent Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid