title: Expression of constitutive nitric oxide synthase isoforms in varicose vein wall; preliminary results.
abstract: There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpaired t-test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works.
authors: Department of Anatomy, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, Slovakia.
authors: Zora
authors: Z
authors: Haviarová
authors: Andrea
authors: A
authors: Janegová
authors: Pavel
authors: P
authors: Janega
authors: Stefan
authors: S
authors: Durdík
authors: Peter
authors: P
authors: Kováč
authors: Viera
authors: V
authors: Stvrtinová
authors: Peter
authors: P
authors: Mráz