title: Tracing fungi secondary metabolites in Brazil nuts using LC-MS/MS.
abstract: This screening aimed to evaluate quantitatively the occurrence of fungal metabolites in Brazil nuts. Nuts were collected from Agroforest production areas in Amazon basin region. A total of 235 mycotoxins (including the most prominent ones) was screened by a multi-mycotoxin method based on HPLC-MS/MS. The recovery of metabolites by the method was between 56 and 136%. Fifteen mycotoxins were detected and quantified, in at least one sample; namely, aflatoxins (AFB(1), AFB(2), AFG(1), and AFM(1)), sterigmatocystin, methyl-sterigmatocystin, kojic acid, citrinin, cyclosporin A, cyclosporin C, cyclosporin D, cyclosporin H, rugulosin, alternariol-methylether and emodin. This is the first study dealing with the detection of the latter nine metabolites in Brazil nuts. Alternariol-methylether (from 0.75 to 3.2 µg/kg) was the only metabolite detected in all analyzed samples.
authors: IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho / Campus de Gualtar, 4710-057, Braga, Portugal. ofreitas@deb.uminho.pt
authors: Otniel
authors: O
authors: Freitas-Silva
authors: Maria
authors: M
authors: de Lourdes
authors: Mendes
authors: M
authors: de Souza
authors: Armando
authors: A
authors: Venãncio
mesh_terms: D031383:Bertholletia / Q000382:microbiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005658:Fungi / Q000302:isolation & purification* / Q000378:metabolism; D009183:Mycotoxins / Q000032:analysis / Q000302:isolation & purification*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D009183:Mycotoxins