title: Isoliquiritigenin from licorice root suppressed neovascularisation in experimental ocular angiogenesis models.
abstract: OBJECTIVE
  To explore the antiangiogenic property of isoliquiritigenin (ISL) on in vivo and in vitro models.
  
  
  METHODS
  Laboratory investigation.
  
  
  METHODS
  The effect of ISL on angiogenesis development was investigated using ex ovo chick chorioallantoic membrane model. Its effect on pathological angiogenesis was examined by (1) silver nitrate cauterisation-induced corneal neovascularisation in BALB/c mice, followed by topical ISL (0.2-50 μM) and CD31 immunofluorescence of corneal blood vessels; (2) argon laser photocoagulation-induced choroidal neovascularisation in C57BL/6 mice, followed by intravitreal ISL (10-200 μM) and fundus fluorescein angiography and immunofluorescence with Griffonia simplicifolia isolectin-B4 (GSA I-B4); and (3) oxygen-induced retinopathy in C57BL/6J mice pups, followed by intravitreal ISL (1-100 μM) and GSA I-B4 immunofluorescence. The vascular area was quantified and analysed by one-way analysis of variance and Student t test. Expression of vascular endothelial growth factor (VEGF) and pigment-epithelium-derived factor in human umbilical vein endothelial cells was analysed by western blotting.
  
  
  RESULTS
  Ex ovo chick chorioallantoic membrane assay showed that ISL dose-dependently suppressed VEGF-induced vessel growth. In vivo experiments illustrated that topical ISL alleviated corneal neovascularisation (IC(50)=7.14 μM, day 7) and intravitreal ISL reduced vessel leakage and GSA I-B4-positive vascular area in choroidal and retinal neovascularisation. ISL was found to dose-dependently suppress VEGF and induce pigment epithelium derived factor expression in cultured endothelial cells.
  
  
  CONCLUSIONS
  Using various experimental models of ocular neovascularisation, the authors have demonstrated that ISL from licorice extract has an antiangiogenic effect. The authors' findings suggest that ISL may be a potential antiangiogenic molecule in the development of therapy for neovascularisation diseases.
authors: Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong.
authors: Vishal
authors: V
authors: Jhanji
authors: Huanming
authors: H
authors: Liu
authors: Kasin
authors: K
authors: Law
authors: Vincent Yau-Wing
authors: VY
authors: Lee
authors: Shao-Fen
authors: SF
authors: Huang
authors: Chi-Pui
authors: CP
authors: Pang
authors: Gary Hin-Fai
authors: GH
authors: Yam
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D002478:Cells, Cultured; D047188:Chalcones / Q000494:pharmacology*; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D020256:Choroidal Neovascularization / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016510:Corneal Neovascularization / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005136:Eye Proteins / Q000096:biosynthesis / Q000187:drug effects; D005260:Female; D005451:Fluorescein Angiography; D005455:Fluorescent Antibody Technique; D005654:Fundus Oculi; D006035:Glycyrrhiza; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000187:drug effects; D009883:Ophthalmic Solutions; D010944:Plants; D015861:Retinal Neovascularization / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015843:Serpins / Q000096:biosynthesis / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D047188:Chalcones; D004791:Enzyme Inhibitors; D005136:Eye Proteins; D009414:Nerve Growth Factors; D009883:Ophthalmic Solutions; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C040920:isoliquiritigenin