title: Structural characterization and antioxidant properties of an exopolysaccharide produced by the mangrove endophytic fungus Aspergillus sp. Y16.
abstract: A homogeneous exopolysaccharide, designated As1-1, was obtained from the culture medium of the mangrove endophytic fungus Aspergillus sp. Y16 and purified by anion-exchange and gel-permeation chromatography. Results of chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance (1D and 2D NMR) spectroscopy showed that As1-1 was mainly composed of mannose with small amounts of galactose, and that its molecular weight was about 15 kDa. The backbone of As1-1 mainly consists of (1→2)-linked α-d-mannopyranose units, substituted at C-6 by the (1→6)-linked α-d-mannopyranose, (1→)-linked β-d-galactofuranose and (1→)-linked β-d-mannopyranose units. As1-1 possessed good in vitro antioxidant activity as evaluated by scavenging assays involving 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals. The investigation demonstrated that As1-1 is an exopolysaccharide different from those of other marine microorganisms, and could be a potential antioxidant and food supplement.
authors: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, People's Republic of China.
authors: Yin
authors: Y
authors: Chen
authors: Wenjun
authors: W
authors: Mao
authors: Hongwen
authors: H
authors: Tao
authors: Weiming
authors: W
authors: Zhu
authors: Xiaohui
authors: X
authors: Qi
authors: Yanli
authors: Y
authors: Chen
authors: Hongyan
authors: H
authors: Li
authors: Chunqi
authors: C
authors: Zhao
authors: Yupin
authors: Y
authors: Yang
authors: Yujiao
authors: Y
authors: Hou
authors: Chunyan
authors: C
authors: Wang
authors: Na
authors: N
authors: Li
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001230:Aspergillus / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D043982:Rhizophoraceae / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides