title: Role of Trypanosoma cruzi peroxiredoxins in mitochondrial bioenergetics.
abstract: Trypanosoma cruzi cytosolic (TcCPx) and mitochondrial tryparedoxin peroxidase (TcMPx) play a fundamental role in H(2)O(2) detoxification. Herein, mitochondrial bioenergetics was evaluated in cells that overexpressed TcCPx (CPx) and TcMPx (MPx) and in pTEX. In MPx, a higher expression was observed for TcCPx, and the same correlation was true for CPx. Differences in H(2)O(2) release among the overexpressing cells were detected when the mitochondrial respiratory chain was inhibited using antimycin A or thenoyltrifluoroacetone. MPx had higher O(2) consumption rates than pTEX and CPx, especially in the presence of oligomycin. In all of the cells, the mitochondrial membrane potential and the ATP levels were similar. Because of the mild uncoupling that was observed in MPx, the presence or induction of a proton transporter in the mitochondrial membrane is suggested when TcMPx is expressed at higher levels. Our results show a possible interplay between the cytosolic and mitochondrial antioxidant systems in a trypanosomatid.
authors: Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Bioquímica, IB, UNICAMP, Campinas, São Paulo, Brazil.
authors: Eduardo de Figueiredo
authors: Ede F
authors: Peloso
authors: Simone Cespedes
authors: SC
authors: Vitor
authors: Luis Henrique Gonzaga
authors: LH
authors: Ribeiro
authors: María Dolores
authors: MD
authors: Piñeyro
authors: Carlos
authors: C
authors: Robello
authors: Fernanda Ramos
authors: FR
authors: Gadelha
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D004734:Energy Metabolism; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D008928:Mitochondria / Q000201:enzymology*; D054464:Peroxiredoxins / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D054464:Peroxiredoxins