title: Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris.
abstract: The cattle tick Rhipicephalus (Boophilus) microplus is responsible for great economic losses. It is mainly controlled chemically, with limitations regarding development of resistance to the chemicals. Vaccines may help control this parasite, thereby reducing tick pesticide use. In this light, we performed subcloning of the gene of the protein Bm86-GC, the homologue protein that currently forms the basis of vaccines (Gavac(TM) and TickGard(PLUS)) that have been developed against cattle ticks. The subcloning was done in the pPIC9 expression vector, for transformation in the yeast Pichia pastoris. This protein was characterized by expression of the recombinant Mut+ strain, which expressed greater quantities of protein. The expressed protein (rBm86-CG) was recognized in the Western-blot assay using anti-Gavac, anti-TickGard, anti-larval extract and anti-rBm86-CG polyclonal sera. The serum produced in cattle vaccinated with the antigen CG rBm86 presented high antibody titers and recognized the native protein. The rBm86-GC has potential relevance as an immunogen for vaccine formulation against cattle ticks.
authors: Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
authors: Rodrigo Casquero
authors: RC
authors: Cunha
authors: Renato
authors: R
authors: Andreotti
authors: Fábio Pereira Leivas
authors: FP
authors: Leite
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D002417:Cattle; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000276:immunology; D010843:Pichia / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology; D048494:Rhipicephalus / Q000276:immunology; D014612:Vaccines / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D000906:Antibodies; C085065:BM86 protein, Boophilus; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines