title: ANCA: A Family of Fluorescent Probes that Bind and Stain Amyloid Plaques in Human Tissue.
abstract: A new family of fluorescent markers containing an Amino Naphthalenyl-2-Cyano-Acrylate (ANCA) motif has been synthesized and evaluated for its capability to associate with aggregated β-amyloid (Aβ) peptides. These fluorescent probes contain a nitrogen donor group that is connected via a naphthalene unit to an electron acceptor motif containing Water Solubilizing Groups (WSG). Chemical modifications were introduced to explore their effect on the capability of the ANCA-based probes to fluorescently label aggregated Aβ peptides. All synthesized probes bind to aggregated Aβ fibrils with low micromolar affinity and fluorescently stain amyloid deposits in human brain tissue from patients with Alzheimer's disease. We found that structural modifications of the WSG site do not affect considerably the binding affinity. However, changes of the nitrogen donor group alter significantly the binding affinity of these probes. Also, increasing the hydrophilicity of the donor group leads to improved contrast between the Aβ deposits and the surrounding tissue in histological staining experiments.
authors: Department of Chemistry & Biochemistry, University of California, San Diego, 9500 Gilman Drive MC: 0358, La Jolla, CA 92093-0358, USA.
authors: Willy M
authors: WM
authors: Chang
authors: Marianna
authors: M
authors: Dakanali
authors: Christina C
authors: CC
authors: Capule
authors: Christina J
authors: CJ
authors: Sigurdson
authors: Jerry
authors: J
authors: Yang
authors: Emmanuel A
authors: EA
authors: Theodorakis