title: Inhibition of HIV-1 reverse transcriptase associated activities by the hydroalcoholic extract of Casimiroa edulis seeds.
abstract: The hydroalcoholic extract obtained from the seeds of Casimiroa edulis cultivated in Sardinia (Italy) have been assayed on the two enzymatic-associated activities of the HIV-1 reverse transcriptase (RT), the RNA-dependent DNA polymerase (RDDP) and the ribonuclease H. In biochemical assays, the extract inhibited both activities in a dose-dependent manner, showing a 10-fold more potent inhibition of the HIV-1 RT RDDP activity. Furthermore, the extract was cytotoxic on K562 cell replication.
authors: Department of Applied Sciences in Biosystems, University of Cagliari, Cagliari, Italy.
authors: Francesca
authors: F
authors: Esposito
authors: Luca
authors: L
authors: Zinzula
authors: Andrea
authors: A
authors: Maxia
authors: Enzo
authors: E
authors: Tramontano
authors: Cinzia
authors: C
authors: Sanna
mesh_terms: D032081:Casimiroa / Q000737:chemistry*; D045744:Cell Line, Tumor; D004789:Enzyme Activation / Q000187:drug effects; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D016914:Ribonuclease H / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D018894:Reverse Transcriptase Inhibitors; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H