title: Telomeres in trisomy 21 amniocytes.
abstract: Individuals with trisomy 21 have an increased risk of developing leukemia and premature dementia. They also have a higher rate of telomere loss. The aim of the study was to compare telomere length and the hTERC gene copy number, which encodes the telomerase RNA subunit, in amniocytes of trisomy 21 conceptions and normal pregnancies. A quantitative fluorescence-in-situ protocol (Q-FISH) was used to compare telomere length in amniocytes cultured from 11 trisomy 21 conceptions and from 14 normal pregnancies. Quantification was conducted using novel computer software. Fluorescence in situ hybridization (FISH) was used to assess the percentage of cells with additional copies of hTERC. We found that the immunofluorescence intensity, which represents telomere length, was significantly lower in amniocytes from trisomy 21 conceptions compared to the control group. The trisomy 21 group had a higher number of cells with additional copies of hTERC. This observation could be one of the cytogenetic parameters that represent a state of genetic instability and might play a role in the pathomechanism of typical features of Down syndrome, such as dementia and malignancy.
authors: Genetics Institute, Meir Medical Center, Kfar Saba, Israel. riki.sukenik @ gmail.com
authors: R
authors: R
authors: Sukenik-Halevy
authors: T
authors: T
authors: Biron-Shental
authors: R
authors: R
authors: Sharony
authors: M D
authors: MD
authors: Fejgin
authors: A
authors: A
authors: Amiel
mesh_terms: D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D016022:Case-Control Studies; D018929:Cell Culture Techniques; D003582:Cytogenetics / Q000379:methods*; D003704:Dementia / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D004314:Down Syndrome / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D018628:Gene Dosage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007938:Leukemia / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012313:RNA / Q000235:genetics*; D012307:Risk Factors; D012984:Software; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000737:chemistry
chemical_list: C422952:telomerase RNA; D012313:RNA; D019098:Telomerase