title: Improvement of the trivalent inactivated flu vaccine using PapMV nanoparticles.
abstract: Commercial seasonal flu vaccines induce production of antibodies directed mostly towards hemaglutinin (HA). Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). Thus, seasonal flu vaccine adjuvanted with PapMV nanoparticles can induce universal protection to influenza, which is a major advancement when facing a pandemic.
authors: Department of Microbiology Infectiology and Immunology, Infectious Disease Research Centre, Laval University, Quebec City, Canada.
authors: Christian
authors: C
authors: Savard
authors: Annie
authors: A
authors: Guérin
authors: Karine
authors: K
authors: Drouin
authors: Marilène
authors: M
authors: Bolduc
authors: Marie-Eve
authors: ME
authors: Laliberté-Gagné
authors: Marie-Christine
authors: MC
authors: Dumas
authors: Nathalie
authors: N
authors: Majeau
authors: Denis
authors: D
authors: Leclerc
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D029441:Carica / Q000821:virology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D053758:Nanoparticles / Q000821:virology*; D012621:Seasons; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D015164:Vaccines, Inactivated / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated; D007371:Interferon-gamma