title: A common carcinogen benzo[a]pyrene causes p53 overexpression in mouse cervix via DNA damage.
abstract: Benzo[a]pyrene (BaP) is cytotoxic and/or genotoxic to lung, stomach and skin tissue in the body. However, the effect of BaP on cervical tissue remains unclear. The present study detected DNA damage and the expression of the p53 gene in BaP-induced cervical tissue in female mice. Animals were intraperitoneally injected and orally gavaged with BaP at the doses of 2.5, 5, and 10mg/kg twice a week for 14 weeks. The single-cell gel electrophoresis (SCGE) assay was used to detect the DNA damage. Immunohistochemistry (IHC) and in situ hybridization (ISH) were used to detect the expression of p53 protein and p53 mRNA, respectively. The results showed that BaP induced a significant and dose-dependent increase of the number of cells with DNA damaged and the tail length as well as Comet tail moment in cervical tissue. The expression level of p53 protein and mRNA was increased. The results demonstrate that BaP may show toxic effect on the cervix by increasing DNA damage and the expression of the p53 gene.
authors: Institute of Cancer Research, Department of Biological Science and Engineering, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University School of Life Science and Technology, Xi'an, Shaanxi 710049, China. gaomeili@mail.xjtu.edu.cn
authors: Meili
authors: M
authors: Gao
authors: Yongfei
authors: Y
authors: Li
authors: Ying
authors: Y
authors: Sun
authors: Jiangang
authors: J
authors: Long
authors: Yu
authors: Y
authors: Kong
authors: Shuiyun
authors: S
authors: Yang
authors: Yili
authors: Y
authors: Wang
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000187:drug effects* / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D016158:Genes, p53; D051379:Mice; D008813:Mice, Inbred ICR; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002273:Carcinogens; D016159:Tumor Suppressor Protein p53; D001564:Benzo(a)pyrene