title: Effect of Achyranthes bidentata polysaccharides on the expression of BCL-2 and bax in hepatic tissues after exhaustive exercise in rats.
abstract: This study aims to assess the effects of Achyranthes bidentata polysaccharides (ABPS) on the expression of bcl-2 and bax in hepatic tissues after exhaustive exercise in order to provide theoretical support for the application of ABPS in the field of sports nutrition. Thirty male Sprague-Dawley rats were randomized into three groups, each consisting of 10 rats: Normal control group (NCG), Exhausting exercises control group (EECG), ABPS treated group (ATG). ABPS were fed orally by gastric intubation to rats of ABPS treated group (ATG) once daily for 7 days. Control animals (EECG and NCG) received the same amount of isotonic sodium chloride solution. Exhaustive exercise was performed on a rodent treadmill. The SP (streptavidin peroxidase) method for immunohistochemical staining was adopted to test the protein expression of bax and bcl-2 in the hepatic tissues of the rats. Exhausting exercises increased bax protein expression of hepatic tissues of rats and bax/bcl-2 ratio dramatically, but a decreased bcl-2 protein expression. In the rats fed ABPS orally by gastric intubation, the bax protein expression and bax/bcl-2 ratio obviously decreased, while bcl-2 protein expression increased. The result indicated that bax and bcl-2 co-regulated the exercise-induced hepatocyte apoptosis. Feeding ABPS orally by gastric intubation to rats can inhibit the hepatocyte apoptosis in exhaustive exercise.
authors: Department of Physical Education, Central South University, Changsha City, Hunan Province, 410083, China. jinyewlin@yahoo.com.cn
authors: Jinyang
authors: J
authors: Lin
authors: Zhuoying
authors: Z
authors: Zhang
authors: Ying
authors: Y
authors: Shan
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000818:Animals; D017209:Apoptosis; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010805:Physical Conditioning, Animal; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D051028:bcl-2-Associated X Protein / Q000187:drug effects* / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; D011134:Polysaccharides; D051028:bcl-2-Associated X Protein