title: Effect of Aspergillus fumigatus and Candida albicans on pro-inflammatory response in cystic fibrosis epithelium.
abstract: BACKGROUND
  The identification of filamentous fungi and/or yeasts in the airway secretions of individuals with cystic fibrosis (CF) is becoming increasingly prevalent; yet the importance of these organisms in relation to underlying inflammation is poorly defined.
  
  
  METHODS
  Cystic fibrosis bronchial epithelial cells (CFBE) and human bronchial epithelial cells (HBE) were co-incubated with Candida albicans whole cells or Aspergillus fumigatus conidia for 24 h prior to the measurement of pro-inflammatory cytokines IL-6 and IL-8 by ELISA.
  
  
  RESULTS
  Treatment of HBE or CFBE with C. albicans whole cells did not alter cytokine secretion. However treatment of CFBE with A. fumigatus conidia resulted in a 1.45-fold increase in IL-6 and a 1.65-fold increase in IL-8 secretion in comparison to basal levels; in contrast there was far less secretion from HBE cells.
  
  
  CONCLUSIONS
  Our data indicate that A. fumigatus infection modulates a pro-inflammatory response in CF epithelial cells while C. albicans does not.
authors: Respiratory Medicine Research Group, Centre for Infection and Immunity, Queens University Belfast, Health Sciences Building, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, UK.
authors: James A
authors: JA
authors: Reihill
authors: John E
authors: JE
authors: Moore
authors: J Stuart
authors: JS
authors: Elborn
authors: Madeleine
authors: M
authors: Ennis
mesh_terms: D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D002176:Candida albicans / Q000302:isolation & purification*; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D007249:Inflammation / Q000382:microbiology*; D020545:Respiratory Mucosa / Q000166:cytology* / Q000382:microbiology*