title: Effects of acupuncture at Taixi acupoint (KI3) on kidney proteome.
abstract: This research explored and identified the protein composition of rat kidneys after acupuncture at the Taixi acupoint (KI3). Twelve adult male Wistar rats were randomly divided into a control group (n = 6) and an acupuncture group (n = 6). Rats in the acupuncture group received electroacupuncture on the bilateral KI3 for seven days. The kidneys were perfused with ice-cold saline and all kidney proteins were isolated. After protein sample preparation, two-dimensional gel electrophoresis (2-DE) was performed. The interesting spots were analyzed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). There were nine protein spots with three-fold up-regulation in the kidney after the acupuncture. NAD-dependent isocitrate dehydrogenase and quinone reductase, the proteins involved in energy metabolism, the reduction of endogenous quinones, chemoprotection, and electrophilic stress, were identified. The data indicated that acupuncture at the KI3 of the kidney meridian of the foot shaoyin was able to increase NAD-dependent isocitrate dehydrogenase and quinone reductase expression in the kidney, and supported the relationship between the kidney and KI3.
authors: College of Acupuncture and Moxibustion, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, PR China.
authors: Chun-Ri
authors: CR
authors: Li
authors: Ze-Dong
authors: ZD
authors: Cheng
authors: Zhi-Xing
authors: ZX
authors: Zhang
authors: Andre
authors: A
authors: Kim
authors: Jong-Myung
authors: JM
authors: Ha
authors: Yuan-Yuan
authors: YY
authors: Song
authors: Jie
authors: J
authors: Zheng
authors: Yi-Guo
authors: YG
authors: Chen
mesh_terms: D026881:Acupuncture / Q000379:methods*; D015669:Acupuncture Points; D000818:Animals; D004558:Electric Stimulation / Q000379:methods*; D015671:Electroacupuncture; D015180:Electrophoresis, Gel, Two-Dimensional; D005528:Foot; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D020543:Proteome; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D011506:Proteins; D020543:Proteome; D009243:NAD; D007521:Isocitrate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone)