title: Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR.
abstract: An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.
authors: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan.
authors: Longzheng
authors: L
authors: Yu
authors: Shoufa
authors: S
authors: Zhang
authors: Wanfeng
authors: W
authors: Liang
authors: Chunmei
authors: C
authors: Jin
authors: Lijun
authors: L
authors: Jia
authors: Yuzi
authors: Y
authors: Luo
authors: Yan
authors: Y
authors: Li
authors: Shinuo
authors: S
authors: Cao
authors: Junya
authors: J
authors: Yamagishi
authors: Yoshifumi
authors: Y
authors: Nishikawa
authors: Suguru
authors: S
authors: Kawano
authors: Kozo
authors: K
authors: Fujisaki
authors: Xuenan
authors: X
authors: Xuan
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D016794:Theileria / Q000235:genetics* / Q000302:isolation & purification; D013801:Theileriasis / Q000453:epidemiology* / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C115173:major piroplasm surface protein, Theileria