title: Neural stem cells maintain their stemness through protein kinase C ζ-mediated inhibition of TRIM32.
abstract: Several studies over the last couple of years have delivered insights into the mechanisms that drive neuronal differentiation. However, the mechanisms that ensure the maintenance of stemness characteristics in neural stem cells over several rounds of cell divisions are still largely unknown. Here, we provide evidence that the neuronal fate determinant TRIM32 binds to the protein kinase C ζ. Through this interaction, TRIM32 is retained in the cytoplasm. However, during differentiation, this interaction is abrogated and TRIM32 translocates to the nucleus to initiate neuronal differentiation by targeting c-Myc for proteasomal degradation.
authors: Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, Stem Cell Biology and Regeneration Group, Münster, Germany.
authors: Anna-Lena
authors: AL
authors: Hillje
authors: Maik M A
authors: MM
authors: Worlitzer
authors: Thomas
authors: T
authors: Palm
authors: Jens C
authors: JC
authors: Schwamborn
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D015536:Down-Regulation; D007150:Immunohistochemistry; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D011493:Protein Kinase C; D044767:Ubiquitin-Protein Ligases; D000099221:Protein Kinase C zeta; C514011:TRIM32 protein, mouse