title: Bile UPLC-MS fingerprinting and bile acid fluxes during human liver transplantation.
abstract: Bile flow restoration is a crucial step in the recovery process post transplantation of the liver. Here, metabolic trajectories based on changes in bile secretion - a known marker of functionality - have been utilised as an approach for discovering bile fluxes during transplantation. A total of ten liver transplants were monitored and from these 68 bile samples from both donors and recipients were collected and analysed using ultra-performance LC-MS in combination with multivariate statistical analysis. Based on the principal component scores constructed from the total bile fingerprint, differentiation of the bile acid concentrations before and after transplantation was detected. A trend was also observed, by constructing metabolic trajectories, whereby the post-transplant profiles approached the position of pre-transplant profiles within 30-60 min of the restoration of bile secretion function. The ten major conjugated bile acid salts were measured and a significant increase in concentrations of taurocholic acid and taurochenodeoxycholic acid were seen after transplantation. In addition, the ratios of secondary bile acids detected in gall bladder and hepatic bile were measured before and after transplantation. This study suggests that bile acid ratios in the donor liver at the pre-transplant and post-transplant stage may be important and that profiling of secreted bile after transplantation may aid clinical assessment and progress post-transplantation.
authors: Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom. cristina.legido_quigley@kcl.ac.uk
authors: Cristina
authors: C
authors: Legido-Quigley
authors: Lindsay
authors: L
authors: McDermott
authors: Hector
authors: H
authors: Vilca-Melendez
authors: Gerard M
authors: GM
authors: Murphy
authors: Nigel
authors: N
authors: Heaton
authors: John C
authors: JC
authors: Lindon
authors: Jeremy K
authors: JK
authors: Nicholson
authors: Elaine
authors: E
authors: Holmes
mesh_terms: D000328:Adult; D000368:Aged; D001646:Bile / Q000737:chemistry* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000032:analysis* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D015999:Multivariate Analysis
chemical_list: D001647:Bile Acids and Salts; D015415:Biomarkers