title: BAFF-R gene induced by IFN-γ in multiple myeloma cells is related to NF-κB signals.
abstract: B-cell activating factor (BAFF) is a potent cell-survival factor expressed in many haematopoietic cells. BAFF regulates B-cell survival, differentiation and proliferation by binding to three tumour necrosis factor receptors: transmembrane activator, calcium modulator and cyclophilin ligand interactor; B-cell maturation antigen; and BAFF receptor (BAFF-R). Although BAFF-R is produced by interferon gamma (IFN-γ), the underlying mechanism remains elusive. In this study, we examined the effects of IFN-γ on BAFF-R expression in cultured human multiple myeloma cells (KM3) both at the transcriptional and posttranscriptional levels. Incubation of KM3 cells with IFN-γ elevated the expression of BAFF-R mRNA and protein levels. IFN-γ elicited marked enhancement of the human BAFF-R promoter activity and nuclear factor kappa B (NF-κB) DNA binding activity. NF-κB dependent on the human BAFF-R gene might be regulated via a transcriptional event through one putative NF-κB site on the BAFF-R gene promoter. These results provide a molecular mechanism for the increase in expression of the BAFF-R gene that is induced by proinflammatory cytokines in responsive cells.
authors: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong, Jiangsu, China.
authors: Xianjuan
authors: X
authors: Shen
authors: Xia
authors: X
authors: Zhang
authors: Guang
authors: G
authors: Xu
authors: Shaoqing
authors: S
authors: Ju
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D053265:B-Cell Activation Factor Receptor / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D019848:Gene Expression Regulation, Archaeal / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009101:Multiple Myeloma / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D000998:Antiviral Agents; D053265:B-Cell Activation Factor Receptor; D016328:NF-kappa B; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA