title: Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells.
abstract: Oral carcinoma is a serious public health problem and the leading cause of head and neck cancer mortality worldwide. Moreover, oral cancer patients often present symptoms at a late stage and show a high recurrence rate after treatment. Therefore, there is an urgent need to identify novel biomarkers for early diagnosis or clinical oral cancer therapy. In this study, we employed a subset of lentiviral short hairpin RNAs targeted against various kinases and phosphatases, designed by The RNAi Consortium, to screen systemically and in a high-throughput manner for potential growth regulators of oral cancer cells. The screen revealed a total of 50 candidate genes, for which more than 90% of growth inhibition in human oral squamous cancer HSC-3 cells was obtained. Furthermore, bioinformatic analysis of these candidate genes identified transforming growth factor-β receptor type II- and fms-related tyrosine kinase 3-related molecular pathways that are involved in NF-κB-mediated growth of HSC-3 cells. These candidate genes may be potential biomarkers for early diagnosis of oral cancer. In addition, these candidate genes represent potential targets for anticancer drug design helping to develop a personalized treatment to combat oral cancer.
authors: Graduate Institute of Life Sciences, National Defense Medical Center, No. 161, Section 6, Min-Chuan East Road, Taipei, Taiwan.
authors: Ming-Han
authors: MH
authors: Yeh
authors: Tzung-Chieh
authors: TC
authors: Tsai
authors: Han-Peng
authors: HP
authors: Kuo
authors: Nai-Wen
authors: NW
authors: Chang
authors: Miao-Rong
authors: MR
authors: Lee
authors: Jing-Gung
authors: JG
authors: Chung
authors: Ming-Hsui
authors: MH
authors: Tsai
authors: Jah-Yao
authors: JY
authors: Liu
authors: Ming-Ching
authors: MC
authors: Kao
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019295:Computational Biology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics; D057166:High-Throughput Screening Assays; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D016086:Lentivirus / Q000235:genetics; D009062:Mouth Neoplasms / Q000201:enzymology / Q000235:genetics*; D016328:NF-kappa B / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics*; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors; D015203:Reproducibility of Results; D055765:Shc Signaling Adaptor Proteins / Q000378:metabolism; D015398:Signal Transduction; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D051941:fms-Like Tyrosine Kinase 3 / Q000037:antagonists & inhibitors
chemical_list: D014408:Biomarkers, Tumor; D016328:NF-kappa B; D034741:RNA, Small Interfering; D018125:Receptors, Transforming Growth Factor beta; C528024:SHC1 protein, human; D055765:Shc Signaling Adaptor Proteins; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1; D010770:Phosphotransferases; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3; D017346:Protein Serine-Threonine Kinases; D051550:I-kappa B Kinase; C496560:IKBKB protein, human; D000077294:Receptor, Transforming Growth Factor-beta Type II; D010744:Phosphoric Monoester Hydrolases