title: Surface plasmon resonance investigation of RNA aptamer-RNA ligand interactions.
abstract: Instruments based on the surface plasmon resonance (SPR) principle allow label-free detection of interactions between targets immobilized at a solid-liquid interface and partners in solution. This method is well suited to determine the kinetic parameters, the equilibrium constant and the stoichiometry of a reaction. Aptamers are ligands identified from random libraries of RNA, DNA or chemically modified oligonucleotides by in vitro selection (SELEX). Aptamers can be raised against a great variety of targets (small molecules, proteins, nucleic acids, cells, viruses, bacteria). SPR is routinely used in our laboratory for the analysis of RNA aptamer-RNA target complexes. To illustrate SPR investigation of such complexes, we describe here methods that were successfully used to monitor the interaction between the trans-activating responsive element of HIV-1 and an RNA aptamer.
authors: ARNA laboratory, University of Bordeaux, F-33000 Bordeaux, France. carmelo.diprimo@inserm.fr
authors: Carmelo
authors: C
authors: Di Primo
authors: Eric
authors: E
authors: Dausse
authors: Jean-Jacques
authors: JJ
authors: Toulmé
mesh_terms: D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000378:metabolism; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008024:Ligands; D058990:Molecular Targeted Therapy / Q000379:methods*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000138:chemical synthesis; D052156:SELEX Aptamer Technique / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands; D034741:RNA, Small Interfering; D012313:RNA; D001710:Biotin; D019809:Streptavidin