title: UW is superior compared with HTK after prolonged preservation of renal grafts.
abstract: BACKGROUND
  In recent clinical studies, the efficacy of histidine-tryptophan-ketoglutarate (HTK) in kidney transplantation was questioned. This study compares the efficacy of University of Wisconsin (UW) and HTK solutions on transplantation outcome.
  
  
  METHODS
  Rat kidneys were preserved for different periods of cold ischemia (CIT). Heat capacity of the solutions, temperature of the grafts, renal function (RF), and histology were assessed before and after transplantation, respectively.
  
  
  RESULTS
  After prolonged CIT, recipient survival was superior in the UW - (100%) compared with the HTK group (10%). In the latter, severe tubular necrosis, DNA damage, and renal inflammation were observed, reflected by an increased KIM-1, IL6, and P-selectin expression. CIT correlated negatively with RF in both groups. RF recovered significantly faster in the UW group. LDH-release and ATP depletion after cold storage of tubular cells were lower in UW than in HTK. Heat capacity was significantly higher for UW than for HTK. Accordingly, renal temperature was lower.
  
  
  CONCLUSIONS
  Prolonged preservation in UW solution results in a better renal function and less tissue damage compared with HTK, possibly due to improved cooling and better cell viability of the graft. The use of HTK for renal allografts should therefore be reconsidered, particularly when CIT is expected to be long.
authors: Department of Medicine V (Nephrology/Endocrinology/Rheumatology), University Medical Center Mannheim, University of Heidelberg, Mannheim, Germany. Simone.hoeger@medma.uni-heidelberg.de
authors: Simone
authors: S
authors: Hoeger
authors: Georgia
authors: G
authors: Lueg
authors: Charalambos
authors: C
authors: Tsagogiorgas
authors: Michael
authors: M
authors: Schneider
authors: Sonja
authors: S
authors: Theisinger
authors: Bastian
authors: B
authors: Theisinger
authors: Johann
authors: J
authors: Fontana
authors: Ruediger
authors: R
authors: Waldherr
authors: Bernhard K
authors: BK
authors: Krämer
authors: Peter
authors: P
authors: Schnuelle
authors: Benito
authors: B
authors: Yard
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D003080:Cold Temperature; D016207:Cytokines / Q000235:genetics; D004249:DNA Damage; D005947:Glucose / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology; D009926:Organ Preservation / Q000379:methods* / Q000401:mortality; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: C039984:Bretschneider cardioplegic solution; D016207:Cytokines; D007328:Insulin; D019188:Organ Preservation Solutions; D012333:RNA, Messenger; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose