title: Identification of a chemoreceptor zinc-binding domain common to cytoplasmic bacterial chemoreceptors.
abstract: We report the identification and characterization of a previously unidentified protein domain found in bacterial chemoreceptors and other bacterial signal transduction proteins. This domain contains a motif of three noncontiguous histidines and one cysteine, arranged as Hxx[WFYL]x(21-28)Cx[LFMVI]Gx[WFLVI]x(18-27)HxxxH(boldface type indicates residues that are nearly 100% conserved). This domain was first identified in the soluble Helicobacter pylori chemoreceptor TlpD. Using inductively coupled plasma mass spectrometry on heterologously and natively expressed TlpD, we determined that this domain binds zinc with a subfemtomolar dissociation constant. We thus named the domain CZB, for chemoreceptor zinc binding. Further analysis showed that many bacterial signaling proteins contain the CZB domain, most commonly proteins that participate in chemotaxis but also those that participate in c-di-GMP signaling and nitrate/nitrite sensing, among others. Proteins bearing the CZB domain are found in several bacterial phyla. The variety of signaling proteins using the CZB domain suggests that it plays a critical role in several signal transduction pathways.
authors: Department of Biomolecular Engineering, University of California at Santa Cruz, Santa Cruz, CA 95064, USA.
authors: Jenny
authors: J
authors: Draper
authors: Kevin
authors: K
authors: Karplus
authors: Karen M
authors: KM
authors: Ottemann
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis; D003001:Cloning, Molecular; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000235:genetics / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000235:genetics; D006639:Histidine / Q000737:chemistry / Q000378:metabolism; D009154:Mutation; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015032:Zinc / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D006639:Histidine; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP; D015032:Zinc; D003545:Cysteine