title: The frataxin homologue Fra plays a key role in intracellular iron channeling in Bacillus subtilis.
abstract: Frataxin homologues are important iron chaperones in eukarya and prokarya. Using a native proteomics approach we were able to identify the structural frataxin homologue Fra (formerly YdhG) of Bacillus subtilis and to quantify its native iron-binding stoichiometry. Using recombinant proteins we could show in vitro that Fra is able to transfer iron onto the B. subtilis SUF system for iron-sulfur cluster biosynthesis. In a four-constituents reconstitution system (including SufU, SufS, Fra and CitB) we observed a Fra-dependent formation of a [4 Fe-4 S] cluster on SufU that could be efficiently transferred onto the target apo-aconitase (CitB). A Δfra deletion mutant showed a severe growth phenotype associated with a broadly disturbed iron homeostasis; this indicates that Fra is a central component of intracellular iron channeling in B. subtilis.
authors: Fachbereich Chemie/Biochemie der Philipps Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors: Alexander G
authors: AG
authors: Albrecht
authors: Hannes
authors: H
authors: Landmann
authors: David
authors: D
authors: Nette
authors: Olaf
authors: O
authors: Burghaus
authors: Florian
authors: F
authors: Peuckert
authors: Andreas
authors: A
authors: Seubert
authors: Marcus
authors: M
authors: Miethke
authors: Mohamed A
authors: MA
authors: Marahiel
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D018832:Molecular Chaperones / Q000378:metabolism; D040901:Proteomics; D000096923:Frataxin
chemical_list: D007501:Iron; D033862:Iron-Binding Proteins; D018832:Molecular Chaperones; D000096923:Frataxin