title: Changes in 5-HT1A receptor in the dorsal raphe nucleus in a rat model of post-traumatic stress disorder.
abstract: Post-traumatic stress disorder (PTSD) is characterized mainly by symptoms of re-experiencing, avoidance and hyperarousal as a consequence of catastrophic and traumatic events that are distinguished from ordinary stressful life events. Single-prolonged stress (SPS) is an established animal model for post-traumatic stress disorder (PTSD). The dorsal raphe nucleus (DR)-serotonin (5-HT) system is markedly affected by swim stress and has been implicated in affective disorders. The 5-HT1A receptor (5-HT1AR) is critically involved in regulating mood and anxiety levels. In this study, we investigated changes in the expression of 5-HT1AR in the DR of rats after SPS that may reveal part of the pathogenesis of PTSD. 5-HT1AR expression in the DR was examined using immunohistochemistry, Western blotting and reverse transcription polymerase chain reaction. The expression of 5-HT1AR in the DR after SPS exposure was increased when compared to that in the control group (P<0.05). These findings indicate an increase in 5-HT1AR in the DR of SPS rats, which may play important roles in the pathogenesis of PTSD rats.
authors: Institute of Pathology and Pathophysiology, and Department of Histology and Embryology, Basic Medical Sciences College, China Medical University, Shenyang, Liaoning 110001, P.R. China.
authors: Fei-Fei
authors: FF
authors: Luo
authors: Fang
authors: F
authors: Han
authors: Yu-Xiu
authors: YX
authors: Shi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011903:Raphe Nuclei / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics / Q000378:metabolism*; D013313:Stress Disorders, Post-Traumatic / Q000378:metabolism* / Q000473:pathology*
chemical_list: D012333:RNA, Messenger; D044282:Receptor, Serotonin, 5-HT1A