title: Effects of TiO2 nanotubes with different diameters on gene expression and osseointegration of implants in minipigs.
abstract: Titanium dioxide (TiO(2)) nanotubes can accelerate the adhesion and differentiation of osteoblasts, yet little is known how this nano-modified implant surface affects osseointegration at molecular level in vivo. The aim of this study was to investigate the effects of TiO(2) nanotubes with different diameters (30 nm, 70 nm and 100 nm) on biological attachment mechanism of implants to bone in vivo by studying the gene expression and bone formation around the implants. The histological features and fluorochrome labeling changes of bone around implants on the non-decalcified sections (at 3, 5 and 8 weeks after implantation) were investigated by using traditional light- and fluorescent microscopy, and the gene expression of alkaline phosphatase (ALP), osterix (Osx), collagen-I (Col-I) and tartrate-resistant acid phosphatase (TRAP) was examined by using real-time PCR at 1, 2, 3, 4 and 5 weeks after implantation. Comparing with machined titanium implants, a significant increase in bone-implant contact (BIC) and gene expression levels was found in the bone attached to implants with TiO(2) nanotubes, especially with 70 nm diameter nanotubes. At the same time, the sequential fluorescent labeling images illustrated dynamic bone deposition. In conclusion, TiO(2) nanotubes can modulate bone formation events at the bone-implant interface as to reach favorable molecular response and osseointegration; in addition, the diameters of nanotubes can be precisely controlled in order to obtain better bone formation.
authors: Department of Prosthodontics, School of Stomatology, Capital Medical University, Beijing, China.
authors: Na
authors: N
authors: Wang
authors: Hongyi
authors: H
authors: Li
authors: Wulong
authors: W
authors: Lü
authors: Jinghui
authors: J
authors: Li
authors: Jinshu
authors: J
authors: Wang
authors: Zhenting
authors: Z
authors: Zhang
authors: Yiran
authors: Y
authors: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D020007:Implants, Experimental; D008297:Male; D043942:Nanotubes / Q000737:chemistry*; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010316:Particle Size; D011897:Random Allocation; D013499:Surface Properties; D013552:Swine; D013556:Swine, Miniature; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C009495:titanium dioxide; D014025:Titanium