title: [Research on the effects of PIAS3 expression on the invasion of glioma TJ905 cells].
abstract: OBJECTIVE
  To investigate the function and possible mechanisms of PIAS3 expression on the invasion of TJ905 cells.
  
  
  METHODS
  PIAS3 overexpression vectors were constructed and PIAS3 siRNA were chemically synthesized, which were separately transfected into TJ905 cells for upregulation or downregulation of PIAS3 expression levels in TJ905 cells. After that, the invasive effects of TJ905 cells were measured by Transwell assay, and the expression of PIAS3, tissue inhibitor of metalloproteinases (TIMP)3, matrix metalloprotease (MMP)-2, and MMP-9 were identified by Western blot.
  
  
  RESULTS
  In vitro transfection efficiency of plasmids and oligonucleotides were separately 85.3% ± 3.1% and 95.1% ± 2.9%. PIAS3 overexpression plasmid transfection in vitro could effectively improve the expression of PIAS3 protein in TJ905 cells and inhibit the invasion of TJ905 cells (P < 0.05), and cell penetration ratio reduced from 87.9% ± 9.3% to 37.3% ± 7.9% compared with control group, while it upregulated TIMP3 and downregulated MMP-2, MMP-9 protein expression (P < 0.05); PIAS3 siRNA transfection could inhibit the PIAS3 protein expression of TJ905 cells and promote the invasion of TJ905 cells (P < 0.05), and cell penetration ratio increased from 83.9% ± 7.1% to 93.2% ± 3.1% compared with control group, while it downregulated TIMP3 and upregulated MMP-2, MMP-9 protein expression (P < 0.05).
  
  
  CONCLUSIONS
  PIAS3 expression is closely related to the invasion properties of glioma TJ905 cells.
authors: Department of Neurosurgery, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215300, China.
authors: Tian-Hong
authors: TH
authors: Pan
authors: Ying-Yi
authors: YY
authors: Wang
authors: Shu-Guang
authors: SG
authors: Zhang
authors: Jun-Xia
authors: JX
authors: Zhang
authors: Rui
authors: R
authors: Li
authors: Wei
authors: W
authors: Yan
authors: Ao-Lin
authors: AL
authors: He
authors: Zhi-Min
authors: ZM
authors: Wang
authors: Lei
authors: L
authors: Shi
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000378:metabolism; D014162:Transfection
chemical_list: D018832:Molecular Chaperones; C484745:PIAS3 protein, human; D050828:Protein Inhibitors of Activated STAT; D034741:RNA, Small Interfering; C088390:TIMP3 protein, human; D019717:Tissue Inhibitor of Metalloproteinase-3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9