title: Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in soil by quantitative reverse transcription-PCR.
abstract: In this study, we evaluated the effect of soil clay content on RNA isolation and on quantitative reverse transcription-PCR (qRT-PCR) quantification of microbial gene transcripts. The amount of clay significantly altered RNA isolation yields and qRT-PCR analyses. Recommendations are made for quantifying microbial gene transcripts in soil samples varying in clay content.
authors: Université de Moncton, Biology Department, Moncton, New Brunswick, Canada.
authors: A
authors: A
authors: Novinscak
authors: M
authors: M
authors: Filion
mesh_terms: D000538:Aluminum Silicates / Q000032:analysis*; D000077215:Clay; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D000538:Aluminum Silicates; D012333:RNA, Messenger; D012987:Soil; D000077215:Clay