title: Calcineurin subunit B is an immunostimulatory protein and acts as a vaccine adjuvant inducing protective cellular and humoral responses against pneumococcal infection.
abstract: Protective immunity involves a dynamic balance between humoral and cellular immune responses. In the present work we demonstrated that recombinant human calcineurin subunit B (rhCnB) stimulated the expression of the surface molecules CD83, CD80, CD86, CD40, and HLA-DR. It also promoted secretion of inflammatory cytokines IL-6, TNF-α, and IL-1β by human PBMC-derived dendritic cells. In in vivo experiments, splenocytes from BALB/c mice immunized with pneumolysin plus rhCnB contained a higher percentage of CD3(+)CD4(+) T lymphocytes, produced more antigen-specific splenocyte proliferation activity, and had higher anti-pneumolysin immunoglobulin G (IgG) titers. Transcript levels of cytokines such as IL-4, IL-10, and IFN-γ in the splenocytes were also upregulated when in vitro stimulated with pneumolysin. Thus, rhCnB promoted a mixed Th1/Th2 type immune response when given together with the specific antigen PN. RhCnB could have potential as a prophylactic vaccine adjuvant.
authors: Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, Beijing 100875, PR China.
authors: Jing
authors: J
authors: Li
authors: Minling
authors: M
authors: Hu
authors: Junxia
authors: J
authors: Guo
authors: Zhenyi
authors: Z
authors: Su
authors: Qun
authors: Q
authors: Wei
mesh_terms: D000276:Adjuvants, Immunologic / Q000378:metabolism; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D019703:Calcineurin / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011008:Pneumococcal Infections / Q000276:immunology* / Q000517:prevention & control; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000378:metabolism; D022541:Streptococcal Vaccines; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000472:pathogenicity; D013301:Streptolysins / Q000008:administration & dosage / Q000276:immunology / Q000378:metabolism; D058408:Th1-Th2 Balance / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D022541:Streptococcal Vaccines; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; D019703:Calcineurin; C503672:PPP3R2 protein, human