title: Distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in Culex tarsalis Mosquitoes from western Canada with viruses isolated in California and Colorado.
abstract: In a previous study, a new flavivirus genome sequence was identified in Culex tarsalis mosquitoes obtained in Alberta, Canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. Nonstructural protein 5-encoding sequences amplified from Cx. tarsalis pools from western Canada have shown a high similarity to genome sequences of novel flaviviruses isolated from mosquitoes in California and Colorado. Despite wide distribution of this virus, designated Calbertado virus, strains demonstrate a high degree of nonstructural protein 5 nucleotide (> 90%) and amino acid (> 97%) identity. The ecology and geographic range of Calbertado virus warrants further study because it may potentially influence transmission of mosquito-borne flaviviruses, including important human pathogens such as West Nile and Saint Louis encephalitis viruses.
authors: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada. shaun.tyler@phac-aspc.gc.ca
authors: Shaun
authors: S
authors: Tyler
authors: Bethany G
authors: BG
authors: Bolling
authors: Carol D
authors: CD
authors: Blair
authors: Aaron C
authors: AC
authors: Brault
authors: Kanti
authors: K
authors: Pabbaraju
authors: M Veronica
authors: MV
authors: Armijos
authors: David C
authors: DC
authors: Clark
authors: Charles H
authors: CH
authors: Calisher
authors: Michael A
authors: MA
authors: Drebot
mesh_terms: D000416:Alberta; D000818:Animals; D001483:Base Sequence; D002140:California; D003120:Colorado; D009033:Culicidae / Q000821:virology*; D017931:DNA Primers; D005416:Flavivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012367:RNA, Viral