title: Combined analysis of EPHX1, GSTP1, GSTM1 and GSTT1 gene polymorphisms in relation to chronic obstructive pulmonary disease risk and lung function impairment.
abstract: Smoking is considered as the major causal factor of chronic obstructive pulmonary disease (COPD). Nevertheless, a minority of chronic heavy cigarette smokers develops COPD. This suggests important contribution of other factors such as genetic predisposing. Our objective was to investigate combined role of EPHX1, GSTP1, M1 and T1 gene polymorphisms in COPD risk, its phenotypes and lung function impairment. Prevalence of EPHX1, GSTP1, M1 and T1 gene polymorphisms were assessed in 234 COPD patients and 182 healthy controls from Tunisia. Genotypes of EPHX1 (Tyr113His; His139Arg) and GSTP1 (Ile105Val; Ala114Val) polymorphisms were performed by PCR-RFLP, while the deletion in GSTM1 and GSTT1 genes was determined using multiplex PCR. Analysis of combinations showed a significant association of 113His/His EPHX1/null-GSTM1 (OR=4.07) and null-GSTM1/105Val/Val GSTP1 (OR =3.56) genotypes with increased risk of COPD (respectively P=0.0094 and P=0.0153). The null-GSTM1/ null-GSTT1, 105Val/Val GSTP1/null GSTT1, 113His/His EPHX1/null-GSTM1 and null-GSTM1/105Val/Val GSTP1 genotypes were related to emphysema (respectively P=0.01; P=0.009; P=0.008 and P=0.001). Combination of 113His/His EPHX1/null-GSTM1 genotypes showed a significant association with the decrease of Δ FEV1 in patients (P =0.028).In conclusion, our results suggest combined EPHX1, GSTP1, GSTM1 and GSTT1 genetic polymorphisms may play a significant role in the development of COPD, emphysema and decline of the lung function.
authors: Biochemistry and Molecular Biology Laboratory, Monastir, Tunisia. lakhdarramzi@yahoo.fr
authors: Ramzi
authors: R
authors: Lakhdar
authors: Sabri
authors: S
authors: Denden
authors: Jalel
authors: J
authors: Knani
authors: Nadia
authors: N
authors: Leban
authors: Houria
authors: H
authors: Daimi
authors: Mohsen
authors: M
authors: Hassine
authors: Gérard
authors: G
authors: Lefranc
authors: Jemni Ben
authors: JB
authors: Chibani
authors: Amel Haj
authors: AH
authors: Khelil
mesh_terms: D000368:Aged; D015992:Body Mass Index; D016022:Case-Control Studies; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D017353:Gene Deletion; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease / Q000235:genetics; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D029424:Pulmonary Disease, Chronic Obstructive / Q000453:epidemiology / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D014416:Tunisia / Q000453:epidemiology
chemical_list: D004851:Epoxide Hydrolases; D005819:Genetic Markers; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human; C522330:EPHX1 protein, human