title: Binding of group B streptococcal phosphoglycerate kinase to plasminogen and actin.
abstract: The glycolytic enzyme, phosphoglycerate kinase (PGK) of group B streptococci (GBS), has previously been identified as expressed on the GBS cell surface. The data presented describes the ability of group B streptococcal phosphoglycerate kinase (GBS-PGK) to bind to plasminogen and to bind actin. GBS-PGK binding to plasminogen was inhibited by the lysine analogue, 6-aminocaproic acid, suggesting plasminogen binding is achieved through GBS-PGK lysine residues. In addition to GBS-PGK surface expression, GBS-PGK was also found to be released from the bacterial cell suggesting GBS-PGK may affect its environment independent of GBS. To determine the effect of GBS-PGK on the actin cytoskeleton within a host cell, GBS-PGK attached to green fluorescent protein was transfected into and expressed in HeLa cells. Transfected GBS-PGK disrupted the actin cytoskeleton resulting in a compact or ovoid shaped HeLa cell rather than a typical epithelioid appearance. In conclusion, we have shown GBS-PGK binds to plasminogen and actin. We have also shown that GBS-PGK can be released from the bacterial cell and that transfected GBS-PGK can alter the epithelial cell cytoskeleton.
authors: The Department of Laboratory Medicine and Pathology, The University of Alberta, Edmonton, Alberta, Canada. T.Boone@provlab.ab.ca
authors: Tyler J
authors: TJ
authors: Boone
authors: Carey-Ann D
authors: CA
authors: Burnham
authors: Gregory J
authors: GJ
authors: Tyrrell
mesh_terms: D000199:Actins / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010735:Phosphoglycerate Kinase / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D013292:Streptococcus agalactiae / Q000201:enzymology*
chemical_list: D000199:Actins; D010958:Plasminogen; D010735:Phosphoglycerate Kinase