title: Protein profiling of genomic instability in endometrial cancer.
abstract: DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n = 5), diploid (n = 7), and aneuploid (n = 7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-κB as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer.
authors: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors: Timo
authors: T
authors: Gemoll
authors: Jens K
authors: JK
authors: Habermann
authors: Johanna
authors: J
authors: Lahmann
authors: Silke
authors: S
authors: Szymczak
authors: Caroline
authors: C
authors: Lundgren
authors: Nana K
authors: NK
authors: Bündgen
authors: Thomas
authors: T
authors: Jungbluth
authors: Britta
authors: B
authors: Nordström
authors: Susanne
authors: S
authors: Becker
authors: Marta I
authors: MI
authors: Lomnytska
authors: Hans-Peter
authors: HP
authors: Bruch
authors: Andreas
authors: A
authors: Ziegler
authors: Ulf
authors: U
authors: Hellman
authors: Gert
authors: G
authors: Auer
authors: Uwe J
authors: UJ
authors: Roblick
authors: Hans
authors: H
authors: Jörnvall
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D042822:Genomic Instability; D006801:Humans; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D040081:Protein Array Analysis; D040901:Proteomics; D014746:Vimentin / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D014746:Vimentin