title: Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus.
abstract: The subcellular localization of the 11-kDa protein (p11) encoded by ORF3 of Garlic virus X (GarVX; genus Allexivirus, family Alphaflexiviridae) was examined by confocal microscopy. Granules with intense fluorescence were visible on the endoplasmic reticulum when p11 fused with green or red fluorescent protein (GFP or RFP) was expressed in epidermal cells of Nicotiana benthamiana. Moreover, the p11-RFP granules moved in the cytoplasm, along the cell periphery and through the cell membranes to adjacent cells. A 17-kDa protein (p17) of garlic interacting with p11 was identified by yeast two-hybridization and bimolecular fluorescence complementation assay. When p17 fused to GFP was expressed in epidermal cells of N. benthamiana, it localized to the nucleolus. However, in the presence of GarVX p11, the distribution of p17 changed to that of p11, but did not appear to affect the pattern of movement of p11.
authors: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MOA and Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors: Yuwen
authors: Y
authors: Lu
authors: Fei
authors: F
authors: Yan
authors: Wei
authors: W
authors: Guo
authors: Hongying
authors: H
authors: Zheng
authors: Lin
authors: L
authors: Lin
authors: Jiejun
authors: J
authors: Peng
authors: Michael J
authors: MJ
authors: Adams
authors: Jianping
authors: J
authors: Chen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002466:Cell Nucleolus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005737:Garlic / Q000378:metabolism* / Q000821:virology*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins