title: Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12.
abstract: Strain Yw12, isolated from activated sludge, could completely degrade and utilize methyl parathion as the sole carbon, phosphorus and energy sources for growth in the basic salt media. It could also completely degrade and utilize p-nitrophenol as the sole carbon and energy sources for growth in the minimal salt media. Phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis of 16S rRNA sequence showed that this strain belongs to the genus of Agrobacterium sp. Response surface methodology was used to optimize degradation conditions. Under its optimal degradation conditions, 50 mg l(-1) MP was completely degraded within 2 h by strain Yw12 and the degradation product PNP was also completely degraded within 6 h. Furthermore, strain Yw12 could also degrade phoxim, methamidophos, chlorpyrifos, carbofuran, deltamethrin and atrazine when provided as the sole carbon and energy sources. Enzymatic analysis revealed that the MP degrading enzyme of strain Yw12 is an intracellular enzyme and is expressed constitutively. These results indicated that strain Yw12 might be used as a potential and effective organophosphate pesticides degrader for bioremediation of contaminated sites.
authors: Graduate School, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie, Beijing, China.
authors: Shenghui
authors: S
authors: Wang
authors: Chen
authors: C
authors: Zhang
authors: Yanchun
authors: Y
authors: Yan
mesh_terms: D060054:Agrobacterium / Q000201:enzymology* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000378:metabolism; D001673:Biodegradation, Environmental; D006867:Hydrolases / Q000378:metabolism; D007306:Insecticides / Q000378:metabolism*; D007700:Kinetics; D008743:Methyl Parathion / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D010802:Phylogeny; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000096:biosynthesis; D012336:RNA, Ribosomal, 16S / Q000096:biosynthesis; D012722:Sewage / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; D009596:Nitrophenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D012989:Soil Pollutants; D008743:Methyl Parathion; D006867:Hydrolases; C024836:4-nitrophenol