title: Discovery and characterization of the Crustacean hyperglycemic hormone precursor related peptides (CPRP) and orcokinin neuropeptides in the sinus glands of the blue crab Callinectes sapidus using multiple tandem mass spectrometry techniques.
abstract: The crustacean sinus gland (SG) is a well-defined neuroendocrine site that produces numerous hemolymph-borne agents including the most complex class of endocrine signaling molecules-neuropeptides. Via a multifaceted mass spectrometry (MS) approach, 70 neuropeptides were identified including orcokinins, orcomyotropin, crustacean hyperglycemic hormone (CHH) precursor-related peptides (CPRPs), red pigment concentrating hormone (RPCH), pigment dispersing hormone (PDH), proctolin, RFamides, RYamides, and HL/IGSL/IYRamide. Among them, 15 novel orcokinins, 9 novel CPRPs, 1 novel orcomyotropin, 1 novel Ork/Orcomyotropin-related peptide, and 1 novel PDH were de novo sequenced via collision induced dissociation (CID) from the SG of a model organism Callinectes sapidus. Electron transfer dissociation (ETD) was used for sequencing of intact CPRPs due to their large size and higher charge state. Capillary isoelectric focusing (CIEF) was employed for separation of members of the orcokinin family, which is one of the most abundant neuropeptide families observed in the SG. Collectively, our study represents the most complete characterization of neuropeptides in the SG and provides a foundation for future investigation of the physiological function of neuropeptides in the SG of C. sapidus.
authors: Department of Chemistry, University of Wisconsin, 777 Highland Avenue, Madison, Wisconsin 53705-2222, United States.
authors: Limei
authors: L
authors: Hui
authors: Robert
authors: R
authors: Cunningham
authors: Zichuan
authors: Z
authors: Zhang
authors: Weifeng
authors: W
authors: Cao
authors: Chenxi
authors: C
authors: Jia
authors: Lingjun
authors: L
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000737:chemistry* / Q000378:metabolism; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification; D011498:Protein Precursors / Q000737:chemistry* / Q000302:isolation & purification; D016415:Sequence Alignment; D053719:Tandem Mass Spectrometry
chemical_list: D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D011498:Protein Precursors; C061766:hyperglycemic hormone precursor, crustacean; C079067:orcokinin