title: Distinct metalloproteinase excretion patterns in focal segmental glomerulosclerosis.
abstract: Metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9) degrade type IV collagen, and represent important tissue remodeling enzymes in several kidney disorders. In this study, we measured urinary levels of MMP-2, MMP-9, and the tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in patients with steroid-sensitive nephrotic syndrome (SSNS, n = 18, median age 5) and focal segmental glomerulosclerosis (FSGS, n = 16, median age 15). We found that urinary concentrations of MMP-2, MMP-9, TIMP-1, and TIMP-2 were significantly elevated in FSGS patients as compared to SSNS in both relapse and remission (p < 0.002). Furthermore, urinary levels of these enzymes are increased early on in the FSGS disease process (chronic kidney disease stages 1 and 2). The findings from this pilot study suggest that MMPs and TIMPs have the potential to represent candidate, early non-invasive biomarkers for diagnosis and/or response to therapy. In addition, they may represent therapeutic targets for preventing chronic kidney disease progression in FSGS.
authors: Division of Nephrology & Hypertension, Cincinnati Children's Hospital and Medical Center, 3333 Burnet Ave, MLC 7022, Cincinnati, OH 45229, USA. kimberly.czech@cchmc.org
authors: Kimberly A
authors: KA
authors: Czech
authors: Michael
authors: M
authors: Bennett
authors: Prasad
authors: P
authors: Devarajan
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005923:Glomerulosclerosis, Focal Segmental / Q000201:enzymology / Q000652:urine*; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000652:urine*; D020780:Matrix Metalloproteinase 9 / Q000652:urine*; D009404:Nephrotic Syndrome / Q000652:urine; D010865:Pilot Projects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000652:urine*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000652:urine*
chemical_list: D015415:Biomarkers; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9