title: Electrospun nanofibrous scaffolds for controlled release of adeno-associated viral vectors.
abstract: The integration of viral gene delivery with key features of biomaterial scaffolds that modulate viral delivery in a controlled manner offers a promising strategy for numerous tissue engineering applications. In this study adeno-associated virus (AAV), which is widely utilized in human gene therapy as a gene carrier due to its safety and efficient gene delivery capability, was encapsulated within electrospun nanofibrous scaffolds composed of blended mixtures of elastin-like polypeptides (ELP) and poly (ε-caprolactone) (PCL) and was employed to transduce fibroblasts adherent on the scaffolds. Combinatorial interactions between ELP and PCL chains upon physical blending significantly altered the mechanical properties (i.e. wettability, elastic modulus, strain, etc.) of the ELP/PCL composites, thus providing key tools to mediate controlled release of AAV vectors and robust cellular transduction on the fibrous scaffolds. The ability of ELP/PCL composites to manipulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.
authors: Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors: Slgirim
authors: S
authors: Lee
authors: Jung-Suk
authors: JS
authors: Kim
authors: Hun Su
authors: HS
authors: Chu
authors: Go-Woon
authors: GW
authors: Kim
authors: Jong-In
authors: JI
authors: Won
authors: Jae-Hyung
authors: JH
authors: Jang
mesh_terms: D000818:Animals; D000229:Dependovirus; D004549:Elastin / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D014161:Transduction, Genetic / Q000379:methods*
chemical_list: D010455:Peptides; D011091:Polyesters; C016240:polycaprolactone; D004549:Elastin