title: [Effects of CD74 gene on IFNγR gene expression in MG TEC].
abstract: OBJECTIVE
  To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell(TEC).
  
  
  METHODS
  PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.
  
  
  RESULTS
  Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells.
  
  
  CONCLUSIONS
  CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.
authors: Department of Microbiology and Immunology, Zhengzhou University, Zhengzhou, China. liruqian@yahoo.com.cn
authors: Qian-Ru
authors: QR
authors: Li
authors: Wen-Qiao
authors: WQ
authors: Zang
authors: Feng
authors: F
authors: Gao
authors: Ying
authors: Y
authors: Du
authors: Qing-Yong
authors: QY
authors: Zhang
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D056747:Immunomodulation / Q000235:genetics; D008297:Male; D009157:Myasthenia Gravis / Q000235:genetics* / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017471:Receptors, Interferon / Q000235:genetics*; D013950:Thymus Gland / Q000473:pathology*; D014162:Transfection; D055815:Young Adult; D000096743:Interferon gamma Receptor
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor; C045084:invariant chain