title: Osteogenic constituents from Pterospermum acerifolium Willd. flowers.
abstract: Phytochemical investigation of ethanol extracts of the Pterospermum acerifolium flowers led to the isolation and identification of two new flavones, 4'-(2-methoxy-4-(1,2,3-trihydroxypropyl) phenoxy luteolin (1) and 5,7,3'-trihydroxy-6-O-β-D-glucopyranosyl flavone (2), and one new lactone, 3,5-dihydroxyfuran-2(5H)-one (3) along with 14 known compounds (4-17). The structure of compounds 1-17 was established based on MS, 1D and 2D NMR, spectroscopic analysis. Eight of these compounds (1-6, 8 and 9) were assessed for osteogenic activity by using primary cultures of rat osteoblast. The compounds 1, 3 and 4 significantly stimulated osteoblast differentiation and mineralization as evident from a marked increase in expression of alkaline phosphatase and alizarin red-S staining of osteoblasts.
authors: Medicinal and Process Chemistry Division, Central Drug Research Institute, CSIR, Lucknow, India.
authors: Preety
authors: P
authors: Dixit
authors: Mohd Parvez
authors: MP
authors: Khan
authors: Gaurav
authors: G
authors: Swarnkar
authors: N
authors: N
authors: Chattopadhyay
authors: Rakesh
authors: R
authors: Maurya
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D047309:Flavones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D019660:Malvaceae / Q000737:chemistry*; D013058:Mass Spectrometry; D008968:Molecular Conformation; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D051381:Rats
chemical_list: C562336:3,5-dihydroxyfuran-2(5H)-one; C562335:4'-(2-methoxy-4-(1,2,3-trihydroxypropyl)phenoxy)luteolin; D000880:Anthraquinones; D047309:Flavones; C004468:Alizarin Red S; D000469:Alkaline Phosphatase; D015107:4-Butyrolactone