title: Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo.
abstract: We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.
authors: University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089, USA.
authors: Jae Youn
authors: JY
authors: Hwang
authors: Zeev
authors: Z
authors: Gross
authors: Harry B
authors: HB
authors: Gray
authors: Lali K
authors: LK
authors: Medina-Kauwe
authors: Daniel L
authors: DL
authors: Farkas
mesh_terms: D000465:Algorithms; D000818:Animals; D003287:Contrast Media / Q000493:pharmacokinetics; D016903:Drug Monitoring / Q000379:methods*; D005260:Female; D005708:Gallium / Q000493:pharmacokinetics; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D013050:Spectrometry, Fluorescence; D014183:Transplantation, Heterologous
chemical_list: D003287:Contrast Media; D005708:Gallium; D018719:Erb-b2 Receptor Tyrosine Kinases; C524056:Erbb2 protein, mouse