title: Integrated immunoassay using tuneable surface acoustic waves and lensfree detection.
abstract: The diagnosis of infectious diseases in the Developing World is technologically challenging requiring complex biological assays with a high analytical performance, at minimal cost. By using an opto-acoustic immunoassay technology, integrating components commonly used in mobile phone technologies, including surface acoustic wave (SAW) transducers to provide pressure driven flow and a CMOS camera to enable lensfree detection technique, we demonstrate the potential to produce such an assay. To achieve this, antibody functionalised microparticles were manipulated on a low-cost disposable cartridge using the surface acoustic waves and were then detected optically. Our results show that the biomarker, interferon-γ, used for the diagnosis of diseases such as latent tuberculosis, can be detected at pM concentrations, within a few minutes (giving high sensitivity at a minimal cost).
authors: Division of Biomedical Engineering, University of Glasgow, Oakfield Avenue, Glasgow, UK, G12 8LT.
authors: Yannyk
authors: Y
authors: Bourquin
authors: Julien
authors: J
authors: Reboud
authors: Rab
authors: R
authors: Wilson
authors: Yi
authors: Y
authors: Zhang
authors: Jonathan M
authors: JM
authors: Cooper
mesh_terms: D000162:Acoustics; D015415:Biomarkers / Q000032:analysis; D040421:Cell Phone; D006801:Humans; D007118:Immunoassay; D007371:Interferon-gamma / Q000032:analysis*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014376:Tuberculosis / Q000175:diagnosis / Q000276:immunology
chemical_list: D015415:Biomarkers; D007371:Interferon-gamma