title: Inhibition of pre-B cell colony-enhancing factor attenuates inflammation and apoptosis induced by pandemic H1N1 2009 in lung endothelium.
abstract: The recent pandemic influenza A (H1N1 2009) virus infection has caused acute lung injury in susceptible population resulting in high mortality in ICU patients. In this report, we observed the effect of pre-B cell colony-enhancing factor (PBEF) on the inflammation and apoptosis in H1N1-infected human pulmonary microvascular endothelial cells (HPMECs). We constructed an in vitro HPMEC monolayer model. The results showed that H1N1 2009 induced the increased expression of inflammatory cytokines (IL-6/IL-8/TNF-α/IP-10) and apoptosis factors (FasL/TRAIL) in infected HPMECs. However, PBEF silencing with siRNA inhibited the expression of some inflammatory cytokines and decreased the apoptosis mediated by FasL. We conclude that PBEF might be partially responsible for the localized inflammatory response to H1N1 2009 in the lung microvascular endothelium and the H1N1-induced endothelial cell apoptosis probably through the FasL-mediated pathway.
authors: Department of Respiratory Medicine, Jinling Hospital, Nanjing University School of Medicine, 305 East Zhongshan Road, Nanjing 210002, China.
authors: Wei
authors: W
authors: Gao
authors: Qing
authors: Q
authors: Mao
authors: Ai-Wen
authors: AW
authors: Feng
authors: Hui-Ming
authors: HM
authors: Sun
authors: Wen-Kui
authors: WK
authors: Sun
authors: Xin
authors: X
authors: Lu
authors: Xin
authors: X
authors: Su
authors: Yi
authors: Y
authors: Shi
mesh_terms: D055371:Acute Lung Injury / Q000201:enzymology / Q000473:pathology / Q000821:virology; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000096:biosynthesis / Q000502:physiology; D004285:Dogs; D055785:Gene Knockdown Techniques / Q000379:methods; D006801:Humans; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000502:physiology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000472:pathogenicity*; D007251:Influenza, Human / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D008833:Microcirculation / Q000235:genetics / Q000502:physiology; D054409:Nicotinamide Phosphoribosyltransferase / Q000037:antagonists & inhibitors* / Q000502:physiology*; D058873:Pandemics; D020545:Respiratory Mucosa / Q000098:blood supply / Q000201:enzymology* / Q000473:pathology
chemical_list: D051017:Apoptosis Regulatory Proteins; D016207:Cytokines; D018836:Inflammation Mediators; D054409:Nicotinamide Phosphoribosyltransferase