title: Ecotoxicological assessment of TiO2 byproducts on the earthworm Eisenia fetida.
abstract: The increasing production of nanomaterials will in turn increase the release of nanosized byproducts to the environment. The aim of this study was to evaluate the behaviour, uptake and ecotoxicity of TiO(2) byproducts in the earthworm Eisenia fetida. Worms were exposed to suspensions containing 0.1, 1 and 10 mg/L of byproducts for 24 h. Size of TiO(2) byproducts showed aggregation of particles up to 700 μm with laser diffraction. Only worms exposed at 10 mg/L showed bioaccumulation of titanium (ICP-AES), increasing expression of metallothionein and superoxide dismutase mRNA (Real-time PCR) and induction of apoptotic activity (Apostain and TUNEL). TiO(2) byproducts did not induce cytotoxicity on cœlomocytes, but a significant decrease of phagocytosis was observed starting from 0.1 mg/L. In conclusion, bioaccumulation of byproducts and their production of reactive oxygen species could be responsible for the alteration of the antioxidant system in worms.
authors: Laboratoire Interactions Ecotoxicité, Biodiversité, Ecosystèmes, Université Paul Verlaine - Metz, CNRS UMR 7146, Rue du Général Delestraint, 57070 Metz, France. emilie.bigorgne@umail.univ-metz.fr
authors: Emilie
authors: E
authors: Bigorgne
authors: Laurent
authors: L
authors: Foucaud
authors: Emmanuel
authors: E
authors: Lapied
authors: Jérôme
authors: J
authors: Labille
authors: Céline
authors: C
authors: Botta
authors: Catherine
authors: C
authors: Sirguey
authors: Jaïro
authors: J
authors: Falla
authors: Jérôme
authors: J
authors: Rose
authors: Erik J
authors: EJ
authors: Joner
authors: François
authors: F
authors: Rodius
authors: Johanne
authors: J
authors: Nahmani
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D054750:Ecotoxicology; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism; D049329:Nanostructures / Q000633:toxicity; D009835:Oligochaeta / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014025:Titanium / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D012989:Soil Pollutants; C009495:titanium dioxide; D008668:Metallothionein; D014025:Titanium; D013482:Superoxide Dismutase