title: Creation of a cellooligosaccharide-assimilating Escherichia coli strain by displaying active beta-glucosidase on the cell surface via a novel anchor protein.
abstract: We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h.
authors: Organization of Advanced Science and Technology, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
authors: Tsutomu
authors: T
authors: Tanaka
authors: Hitomi
authors: H
authors: Kawabata
authors: Chiaki
authors: C
authors: Ogino
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics; D018533:Biomass; D002475:Cellobiose / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013053:Spectrophotometry; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D002475:Cellobiose; D001617:beta-Glucosidase