title: Bio-electrocatalysis of NADH and ethanol based on graphene sheets modified electrodes.
abstract: Characterization and application of graphene sheets modified glassy carbon electrodes (graphene/GC) have been presented for the electrochemical bio-sensing. A probe molecule, potassium ferricyanide is employed to study the electrochemical response at the graphene/GC electrode, which shows better electron transfer than graphite modified (graphite/GC) and bare glassy carbon (GC) electrodes. Based on the highly enhanced electrochemical activity of NADH, alcohol dehydrogenase (ADH) is immobilized on the graphene modified electrode and displays a more desirable analytical performance in the detection of ethanol, compared with graphite/GC or GC based bio-electrodes. It also exhibits good performance of ethanol detection in the real samples. From the results of electrochemical investigation, graphene sheets with a favorable electrochemical activity could be an advanced carbon electrode materials for the design of electrochemical sensors and biosensors.
authors: Department of Chemistry and Key Lab of Molecular Engineering of Polymers of Chinese Ministry of Education, Fudan University, Shanghai 200433, PR China.
authors: Kai
authors: K
authors: Guo
authors: Kun
authors: K
authors: Qian
authors: Song
authors: S
authors: Zhang
authors: Jilie
authors: J
authors: Kong
authors: Chengzhong
authors: C
authors: Yu
authors: Baohong
authors: B
authors: Liu
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000434:Alcoholic Beverages / Q000032:analysis; D000818:Animals; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D009243:NAD / Q000032:analysis* / Q000737:chemistry*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D004800:Enzymes, Immobilized; D009243:NAD; D000431:Ethanol; D006108:Graphite; D000426:Alcohol Dehydrogenase