title: Genetic engineering of human pluripotent cells using TALE nucleases.
abstract: Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
authors: The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors: Dirk
authors: D
authors: Hockemeyer
authors: Haoyi
authors: H
authors: Wang
authors: Samira
authors: S
authors: Kiani
authors: Christine S
authors: CS
authors: Lai
authors: Qing
authors: Q
authors: Gao
authors: John P
authors: JP
authors: Cassady
authors: Gregory J
authors: GJ
authors: Cost
authors: Lei
authors: L
authors: Zhang
authors: Yolanda
authors: Y
authors: Santiago
authors: Jeffrey C
authors: JC
authors: Miller
authors: Bryan
authors: B
authors: Zeitler
authors: Jennifer M
authors: JM
authors: Cherone
authors: Xiangdong
authors: X
authors: Meng
authors: Sarah J
authors: SJ
authors: Hinkley
authors: Edward J
authors: EJ
authors: Rebar
authors: Philip D
authors: PD
authors: Gregory
authors: Fyodor D
authors: FD
authors: Urnov
authors: Rudolf
authors: R
authors: Jaenisch
mesh_terms: D001483:Base Sequence; D053595:Embryonic Stem Cells / Q000502:physiology*; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008969:Molecular Sequence Data; D043263:Myosin-Light-Chain Phosphatase / Q000235:genetics; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D014157:Transcription Factors; C433629:homeobox protein PITX3; D004720:Endonucleases; D043263:Myosin-Light-Chain Phosphatase