title: Brassinolide alleviates salt stress and increases antioxidant activity of cowpea plants (Vigna sinensis).
abstract: Soil salinity is one of the most severe factors limiting growth and physiological response in Vigna sinensis plants. Plant salt stress tolerance requires the activation of complex metabolic activities including antioxidative pathways, especially reactive oxygen species and scavenging systems within the cells which can contribute to continued growth under water stress. The present investigation was carried out to study the role of brassinolide in enhancing tolerance of cowpea plants to salt stress (NaCl). Treatment with 0.05 ppm brassinolide as foliar spray mitigated salt stress by inducing enzyme activities responsible for antioxidation, e.g., superoxide dismutase, peroxidase, polyphenol oxidase, and detoxification as well as by elevating contents of ascorbic acid, tocopherol, and glutathione. On the other hand, total soluble proteins decreased with increasing NaCl concentrations in comparison with control plants. However, lipid peroxidation increased with increasing concentrations of NaCl. In addition to, the high concentrations of NaCl (100 and 150 mM) decreased total phenol of cowpea plants as being compared with control plants. SDS-PAGE of protein revealed that NaCl treatments alone or in combination with 0.05 ppm brassinolide were associated with the disappearance of some bands or appearance of unique ones in cowpea plants. Electrophoretic studies of α-esterase, β-esterase, polyphenol oxidase, peroxidase, acid phosphatase, and superoxide dismutase isoenzymes showed wide variations in their intensities and densities among all treatments.
authors: Plant Physiology, Biological and Geological Sciences, Faculty of Education, Ain Shams University, El Makres St. Roxy, Cairo, Egypt.
authors: Ali Abdel Aziz
authors: AA
authors: El-Mashad
authors: Heba Ibrahim
authors: HI
authors: Mohamed
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D060406:Brassinosteroids / Q000494:pharmacology*; D004156:Catechol Oxidase / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D015227:Lipid Peroxidation; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D055049:Salt Tolerance / Q000187:drug effects*; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D060406:Brassinosteroids; D007527:Isoenzymes; D010636:Phenols; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; D017392:Thiobarbituric Acid Reactive Substances; D012965:Sodium Chloride; D004156:Catechol Oxidase; D009195:Peroxidase; D013482:Superoxide Dismutase; D004950:Esterases; D000135:Acid Phosphatase; D005978:Glutathione; D001205:Ascorbic Acid; C023623:brassinolide