title: Pro-inflammatory cytokine expression of spleen dendritic cells in mouse toxoplasmosis.
abstract: Dendritic cells have been known as a member of strong innate immune cells against infectious organelles. In this study, we evaluated the cytokine expression of splenic dendritic cells in chronic mouse toxoplasmosis by tissue cyst-forming Me49 strain and demonstrated the distribution of lymphoid dendritic cells by fluorescence-activated cell sorter (FACS). Pro-inflammatory cytokines, such as IL-1α, IL-1β, IL-6, and IL-10 increased rapidly at week 1 post-infection (PI) and peaked at week 3 PI. Serum IL-10 level followed the similar patterns. FACS analysis showed that the number of CD8α(+)/CD11c(+) splenic dendritic cells increased at week 1 and peaked at week 3 PI. In conclusion, mouse splenic dendritic cells showed early and rapid cytokine changes and may have important protective roles in early phases of murine toxoplasmosis.
authors: Department of Parasitology, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
authors: Ho-Woo
authors: HW
authors: Nam
authors: Hye-Jin
authors: HJ
authors: Ahn
authors: Hyun-Jong
authors: HJ
authors: Yang
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D016207:Cytokines / Q000097:blood* / Q000378:metabolism*; D003713:Dendritic Cells / Q000737:chemistry / Q000276:immunology*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D012376:Rodent Diseases / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013997:Time Factors; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: D039521:CD11c Antigen; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D016207:Cytokines