title: Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging.
abstract: A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.
authors: Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea.
authors: Ju Hui
authors: JH
authors: Park
authors: Kwang Il
authors: KI
authors: Kim
authors: Yong Jin
authors: YJ
authors: Lee
authors: Tae Sup
authors: TS
authors: Lee
authors: Kyeong Min
authors: KM
authors: Kim
authors: Sang-Soep
authors: SS
authors: Nahm
authors: Young-Seo
authors: YS
authors: Park
authors: Gi Jeong
authors: GJ
authors: Cheon
authors: Sang Moo
authors: SM
authors: Lim
authors: Joo Hyun
authors: JH
authors: Kang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004052:Diethylnitrosamine; D005260:Female; D020869:Gene Expression Profiling; D057809:HEK293 Cells; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods*; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000509:alpha-Fetoproteins / Q000235:genetics
chemical_list: D000509:alpha-Fetoproteins; D004052:Diethylnitrosamine; D049409:Luciferases, Firefly