title: Screening siRNAs targeting a novel gene (HA117) and the development of a derivative recombinant adenovirus delivery system.
abstract: A novel gene, HA117, was discovered in our previous work. Using the pSOS-HUS vector method which we designed at previous study, we screened for small interfering RNAs (siRNAs) that targeted HA117. The pSOS-HUS siRNA screening results were verified and a delivery system was developed that contained a recombinant adenovirus carrying DNA templates for the transcription of the HA117 siRNAs. Of five pairs of DNA templates, siRNA transcribed from HAi5 produced the strongest effect against HA117. A recombinant adenovirus containing HAi5 (Ad-HAi5) was successfully constructed and evaluated. This work has laid the foundation for further study of HA117 gene function using RNA interference technology and has showed the pSOS-HUS vector method was successfully utilized as a rapid and effective screen of siRNAs for a target gene.
authors: The Infection and Digestology Department of Affiliated Children's Hospital of Chongqing Medical University, The Children's Hospital, Chongqing, China.
authors: G H
authors: GH
authors: Zheng
authors: Q
authors: Q
authors: Luo
authors: X Q
authors: XQ
authors: Jin
authors: Y X
authors: YX
authors: Guo
authors: Y H
authors: YH
authors: Xu
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D011506:Proteins; D034741:RNA, Small Interfering