title: Physiological diversity within the Kluyveromyces marxianus species [corrected].
abstract: The Kluyveromyces marxianus strains CBS 6556, CBS 397 and CBS 712(T) were cultivated on a defined medium with either glucose, lactose or sucrose as the sole carbon source, at 30 and 37°C. The aim of this work was to evaluate the diversity within this species, in terms of the macroscopic physiology. The main properties evaluated were: intensity of the Crabtree effect, specific growth rate, biomass yield on substrate, metabolite excretion and protein secretion capacity, inferred by measuring extracellular inulinase activity. The strain Kluyveromyces lactis CBS 2359 was evaluated in parallel, since it is the best described Kluyveromyces yeast and thus can be used as a control for the experimental setup. K. marxianus CBS 6556 presented the highest specific growth rate (0.70 h(-1)) and the highest specific inulinase activity (1.65 U mg(-1) dry cell weight) among all strains investigated, when grown at 37°C with sucrose as the sole carbon source. The lowest metabolite formation and highest biomass yield on substrate (0.59 g dry cell weight g sucrose(-1)) was achieved by K. marxianus CBS 712(T) at 37°C. Taken together, the results show a systematic comparison of carbon and energy metabolism among three of the best known K. marxianus strains, in parallel to K. lactis CBS 2359.
authors: Department of Chemical Engineering, University of São Paulo, São Paulo, SP, Brazil. sn-rocha@uol.com.br
authors: Saul Nitsche
authors: SN
authors: Rocha
authors: José
authors: J
authors: Abrahão-Neto
authors: Andreas Karoly
authors: AK
authors: Gombert
mesh_terms: D044822:Biodiversity; D018533:Biomass; D003470:Culture Media / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007716:Kluyveromyces / Q000254:growth & development / Q000502:physiology*; D007785:Lactose / Q000378:metabolism
chemical_list: D003470:Culture Media; D005947:Glucose; D007785:Lactose