title: Characterization of spatial cell distribution in multilayer sheet of human keratinocytes through a stereoscopic cell imaging system.
abstract: A novel system was developed to characterize the spatial distribution of human keratinocytes in cultured epithelial sheet, based on stereoscopic observation of nuclei using a confocal laser microscope in combination with image processing. The presented technique allowed understanding of the proliferative and growth-arrested states of cells in multilayered sheet.
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, Japan.
authors: Mee-Hae
authors: MH
authors: Kim
authors: Naoki
authors: N
authors: Tsubakino
authors: Shogo
authors: S
authors: Kagita
authors: Masahito
authors: M
authors: Taya
authors: Masahiro
authors: M
authors: Kino-oka
mesh_terms: D002467:Cell Nucleus; D002478:Cells, Cultured; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D015603:Keratinocytes / Q000166:cytology*; D018613:Microscopy, Confocal / Q000379:methods*