title: Two-photon polarization microscopy reveals protein structure and function.
abstract: Membrane proteins are a large, diverse group of proteins, serving a multitude of cellular functions. They are difficult to study because of their requirement of a lipid membrane for function. Here we show that two-photon polarization microscopy can take advantage of the cell membrane requirement to yield insights into membrane protein structure and function, in living cells and organisms. The technique allows sensitive imaging of G-protein activation, changes in intracellular calcium concentration and other processes, and is not limited to membrane proteins. Conveniently, many suitable probes for two-photon polarization microscopy already exist.
authors: Laboratory of Cell Biology, Institute of Nanobiology and Structural Biology, Global Change Research Centre, Academy of Sciences of the Czech Republic, Nove Hrdy. lazar@usbe.cas.cz
authors: Josef
authors: J
authors: Lazar
authors: Alexey
authors: A
authors: Bondar
authors: Stepan
authors: S
authors: Timr
authors: Stuart J
authors: SJ
authors: Firestein
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods*; D011487:Protein Conformation; D013329:Structure-Activity Relationship
chemical_list: D008565:Membrane Proteins