title: Myosins and DYNLL1/LC8 in the honey bee (Apis mellifera L.) brain.
abstract: Honey bees have brain structures with specialized and developed systems of communication that account for memory, learning capacity and behavioral organization with a set of genes homologous to vertebrate genes. Many microtubule- and actin-based molecular motors are involved in axonal/dendritic transport. Myosin-Va is present in the honey bee Apis mellifera nervous system of the larvae and adult castes and subcastes. DYNLL1/LC8 and myosin-IIb, -VI and -IXb have also been detected in the adult brain. SNARE proteins, such as CaMKII, clathrin, syntaxin, SNAP25, munc18, synaptophysin and synaptotagmin, are also expressed in the honey bee brain. Honey bee myosin-Va displayed ATP-dependent solubility and was associated with DYNLL1/LC8 and SNARE proteins in the membrane vesicle-enriched fraction. Myosin-Va expression was also decreased after the intracerebral injection of melittin and NMDA. The immunolocalization of myosin-Va and -IV, DYNLL1/LC8, and synaptophysin in mushroom bodies, and optical and antennal lobes was compared with the brain morphology based on Neo-Timm histochemistry and revealed a distinct and punctate distribution. This result suggested that the pattern of localization is associated with neuron function. Therefore, our data indicated that the roles of myosins, DYNLL1/LC8, and SNARE proteins in the nervous and visual systems of honey bees should be further studied under different developmental, caste and behavioral conditions.
authors: Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, MG, Brazil. lkcalabria@gmail.com
authors: Luciana Karen
authors: LK
authors: Calábria
authors: Pablo Marco Veras
authors: PM
authors: Peixoto
authors: Andreia Barcelos
authors: AB
authors: Passos Lima
authors: Leonardo Gomes
authors: LG
authors: Peixoto
authors: Viviane Rodrigues Alves
authors: VR
authors: de Moraes
authors: Renata Roland
authors: RR
authors: Teixeira
authors: Claudia Tavares
authors: CT
authors: Dos Santos
authors: Letícia Oliveira
authors: LO
authors: E Silva
authors: Maria de Fátima Rodrigues
authors: Mde F
authors: da Silva
authors: Ana Alice Diniz
authors: AA
authors: dos Santos
authors: Norberto
authors: N
authors: Garcia-Cairasco
authors: Antônio Roberto
authors: AR
authors: Martins
authors: Enilza Maria
authors: EM
authors: Espreafico
authors: Foued Salmen
authors: FS
authors: Espindola
mesh_terms: D000255:Adenosine Triphosphate; D000818:Animals; D001516:Bees / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000378:metabolism*; D008555:Melitten; D009218:Myosins / Q000378:metabolism*; D016202:N-Methylaspartate; D050600:SNARE Proteins / Q000378:metabolism*; D016708:Synaptophysin / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D050600:SNARE Proteins; D016708:Synaptophysin; D008555:Melitten; D016202:N-Methylaspartate; D000255:Adenosine Triphosphate; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D009218:Myosins