title: Bio-Photonic Sensing Cells over transparent substrates for anti-gestrinone antibodies biosensing.
abstract: In a previous work we introduced the term Bio-Photonic Sensing Cells (BICELLs), referred to periodic networks of nano-pillar suitable for biosensing when are vertically interrogated. In this article, we demonstrate the biosensing capabilities of a type of micrometric size BICELLs made of SU-8 nano-pillars fabricated over transparent substrates. We verify the biochips functionality comparing the theoretical simulations with the experimental results when are optically interrogated in transmission. We also demonstrate a sensitivity enhancement by reducing the pitch among nano-pillars from 800 to 700 nm. Thus, the Limit of Detection achievable in these types of BICELLs is in the order of 64 pg/mL for 700 nm in pitch among nano-pillars in comparison with 292 pg/mL for 800 nm in pitch when are interrogated by Fourier Transform Visible and Infrared Spectrometry. The experiments exhibited a good reproducibility with a relative standard deviation of 0.29% measured within 8 days for a specific concentration. Finally, BICELLs functionality was tested in real conditions with unpurified rabbit serum for detecting anti-gestrinone antibodies, demonstrating the high performance of this type of BICELLs to detect specific antibodies having immobilized the suitable bioreceptors onto the sensing surface.
authors: Centro Láser, Universidad Politécnica de Madrid, Campus Sur, 28031 Madrid, Spain.
authors: F J
authors: FJ
authors: Sanza
authors: M
authors: M
authors: Holgado
authors: F J
authors: FJ
authors: Ortega
authors: R
authors: R
authors: Casquel
authors: D
authors: D
authors: López-Romero
authors: M J
authors: MJ
authors: Bañuls
authors: M F
authors: MF
authors: Laguna
authors: C A
authors: CA
authors: Barrios
authors: R
authors: R
authors: Puchades
authors: A
authors: A
authors: Maquieira
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D005867:Gestrinone / Q000276:immunology*; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D055095:Optics and Photonics / Q000295:instrumentation; D011108:Polymers / Q000737:chemistry; D011817:Rabbits
chemical_list: D000906:Antibodies; D011108:Polymers; D005867:Gestrinone