title: In vitro evaluation of chloroaluminum phthalocyanine nanoemulsion and low-level laser therapy on human skin dermal equivalents and bone marrow mesenchymal stem cells.
abstract: Nanotechnology and tissue engineering are promising scientific fields in the development of advanced materials useful to human health. This article describes the preparation of a nanocarrier for the controlled release of a photosensitizer compound associated with low-level light therapy for skin wound healing treatment and applicable to other skin diseases. A biological model was used as an in vitro skin equivalent based on a three-dimensional culture of fibroblasts and mesenchymal stem cells and denominated by dermal equivalent (DE). Results show that it is possible to use the photomodulation process to control the wound healing in a scratching process and to induce the biomolecules release, both of which are related with the inflammatory wound healing process. In the studies, the MMP-2 and MMP-9 expression from zymography analyses were evaluated. All results showed a dependence on enzymatic activity relating to lowlevel laser applications which indicates a potential application in wound healing processes based on phototherapy and nanotechnology.
authors: Center of Nanotechnology and Tissue Engineers, Photobiology and Photomedicine Research Group, FFCLRP- São Paulo University, 14040-901 Ribeirão Preto-SP, Brazil.
authors: F L
authors: FL
authors: Primo
authors: M B
authors: MB
authors: da Costa Reis
authors: M A
authors: MA
authors: Porcionatto
authors: A C
authors: AC
authors: Tedesco
mesh_terms: D001854:Bone Marrow Cells; D018920:Coculture Techniques; D004655:Emulsions; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007211:Indoles / Q000494:pharmacology* / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D036103:Nanotechnology; D009942:Organometallic Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects / Q000528:radiation effects*; D014945:Wound Healing / Q000187:drug effects / Q000528:radiation effects
chemical_list: D004655:Emulsions; D007211:Indoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C044797:chloroaluminum phthalocyanine; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9