title: Molecular characterization of the probiotic strain Bacillus cereus var. toyoi NCIMB 40112 and differentiation from food poisoning strains.
abstract: Bacillus cereus var. toyoi strain NCIMB 40112 (Toyocerin), a probiotic authorized in the European Union as feed additive for swine, bovines, poultry, and rabbits, was characterized by DNA fingerprinting applying pulsed-field gel electrophoresis and multilocus sequence typing and was compared with reference strains (of clinical and environmental origins). The probiotic strain was clearly characterized by pulsed-field gel electrophoresis using the restriction enzymes Apa I and Sma I resulting in unique DNA patterns. The comparison to the clinical reference strain B. cereus DSM 4312 was done with the same restriction enzymes, and again a clear differentiation of the two strains was possible by the resulting DNA patterns. The use of the restriction enzymes Apa I and Sma I is recommended for further studies. Furthermore, multilocus sequence typing analysis revealed a sequence type (ST 111) that was different from all known STs of B. cereus strains from food poisoning incidents. Thus, a strain characterization and differentiation from food poisoning strains for the probiotic strain was possible.
authors: Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany. guenter.klein@tiho-hannover.de
authors: Günter
authors: G
authors: Klein
mesh_terms: D000821:Animal Feed / Q000382:microbiology*; D001409:Bacillus cereus / Q000145:classification / Q000235:genetics*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D019936:Probiotics; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial