title: ShRNA-mediated gene silencing of heat shock protein 70 inhibits human colon cancer growth.
abstract: Heat shock protein 70 (Hsp70), a chaperone involved in tumor progression, is overexpressed in various human tumors. However, its role in colon cancer progression is not completely understood. In the present study, two shRNA plasmid vectors against Hsp70 were constructed and stably transfected into the colon cancer cell line HT29 to determine the effect of Hsp70 on cell proliferation, cell cycle distribution and cell apoptosis in HT29 cells in vitro, and its effect on xenograft tumor growth and apoptosis in vivo. Cell proliferation was determined using MTT assay. The results revealed that Hsp70 silencing efficiently inhibited the growth of HT29 cells in culture, induced cell cycle arrest at the G1 phase, and significantly increased apoptosis. Moreover, stable clones from the Hsp70 shRNA-2 vector suppressed xenograft tumor growth and enhanced apoptosis in vivo compared with a mock and vector control group. In conclusion, specific Hsp70 shRNA silencing may inhibit colon cancer growth, indicating that Hsp70 silencing is a potential therapeutic strategy for the treatment of colon cancer.
authors: Department of General Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, P.R. China.
authors: Ming-An
authors: MA
authors: Zhong
authors: Hui
authors: H
authors: Zhang
authors: Xin-Yong
authors: XY
authors: Qi
authors: Ai-Guo
authors: AG
authors: Lu
authors: Tian-Geng
authors: TG
authors: You
authors: Wei
authors: W
authors: Gao
authors: Xin-Lai
authors: XL
authors: Guo
authors: Zhu-Qing
authors: ZQ
authors: Zhou
authors: Yao
authors: Y
authors: Yang
authors: Cong-Jun
authors: CJ
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D049109:Cell Proliferation; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005822:Genetic Vectors / Q000235:genetics; D050957:HSP110 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D050957:HSP110 Heat-Shock Proteins; C121993:HSPA4 protein, human; D034741:RNA, Small Interfering