title: Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor.
abstract: Simian virus 40 (SV40) virus-like particles (VLPs) are efficient nanocarriers for gene delivery. VLPs conjugated to human epidermal growth factor (hEGF) were prepared and the cell selectivity of the VLP was examined using human epithelial carcinoma A431 cells, which overexpress the EGF receptor. The endocytic efficiency was determined by the level of Gaussia luciferase activity from the encapsulated protein in hEGF-conjugated VLPs. EGF receptor-mediated endocytosis of hEGF-conjugated VLPs was significantly increased and was confirmed by fluorescence imaging using mCherry encapsulated in hEGF-conjugated VLPs. These results suggest that VLPs of SV40 conjugated to a specific ligand could be used for cell selective gene delivery.
authors: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors: Yuichi
authors: Y
authors: Kitai
authors: Hajime
authors: H
authors: Fukuda
authors: Teruya
authors: T
authors: Enomoto
authors: Yuki
authors: Y
authors: Asakawa
authors: Takahiro
authors: T
authors: Suzuki
authors: Satoshi
authors: S
authors: Inouye
authors: Hiroshi
authors: H
authors: Handa
mesh_terms: D045744:Cell Line, Tumor; D004705:Endocytosis / Q000502:physiology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D018014:Gene Transfer Techniques; D006801:Humans; D015151:Immunoblotting; D008156:Luciferases; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D013539:Simian virus 40; D014771:Virion / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D004815:Epidermal Growth Factor; D008156:Luciferases