title: Natural products modulate Shigella-host-cell interaction.
abstract: This study focused on identifying possible new options derived from natural sources for the treatment of bacterial infections. Several natural products were investigated for their potential in modulating Shigella-host-cell interactions. The proliferation of Shigella sonnei was effectively inhibited inside HEp-2 cells in the presence of 4-methoxycinnamic acid and propolin D. Propolin D also significantly reduced the apoptosis of infected macrophage-like U937 cells and moderately reduced the secretion of interleukin (IL)-1β and IL-18, which probably resulted from the inhibition of invasion plasmid antigen B secretion by this compound. Further characterization showed that propolin D did not prevent escape of Shigella from phagocytic vacuoles, as evidenced by actin-based motility and by the fact that addition of chloroquine did not further reduce the number of intracellular c.f.u. The role of propolin D in modulating autophagy could not be established under the experimental conditions used. As these compounds had no direct anti-Shigella activity in vitro, it was concluded that these compounds modulated Shigella-host-cell interactions by targeting yet-to-be defined mechanisms that provide benefits to host cells.
authors: Centre for Vaccine Development, School of Life Sciences, Xi'an JiaoTong University, Xi'an, PR China.
authors: Dan
authors: D
authors: Xu
authors: Center for Microbiological Preparedness (KCB), Smittskyddsinstitutet, SE-171 82 Solna, Sweden.
authors: Amir
authors: A
authors: Saeed
authors: Centre for Vaccine Development, School of Life Sciences, Xi'an JiaoTong University, Xi'an, PR China.
authors: Yili
authors: Y
authors: Wang
authors: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
authors: Véronique
authors: V
authors: Seidel
authors: Center for Microbiological Preparedness (KCB), Smittskyddsinstitutet, SE-171 82 Solna, Sweden.
authors: Gunnar
authors: G
authors: Sandström
authors: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.|Centre for Vaccine Development, School of Life Sciences, Xi'an JiaoTong University, Xi'an, PR China.
authors: Jun
authors: J
authors: Yu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D002470:Cell Survival / Q000276:immunology; D002934:Cinnamates / Q000494:pharmacology* / Q000627:therapeutic use; D004405:Dysentery, Bacillary / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D056945:Hep G2 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D008826:Microbial Sensitivity Tests; D012764:Shigella sonnei / Q000276:immunology*; D020298:U937 Cells
chemical_list: D002934:Cinnamates; D005419:Flavonoids; D053583:Interleukin-1beta; D016209:Interleukin-8; C063318:4-methoxycinnamic acid