title: Raman detection of localized transferrin-coated gold nanoparticles inside a single cell.
abstract: We investigated the cellular uptake behavior of non-fluorescent metal nanoparticles (NPs) by use of surface-enhanced Raman scattering (SERS) combined with dark-field microscopy (DFM). The uptake of Au NPs inside a single cell could also be identified by DFM first and then confirmed by z-depth-dependent SERS at micrometer resolution. Guided by DFM for the location of Au NPs, an intracellular distribution assay was possible using Raman dyes with unique vibrational marker bands in order to identify the three-dimensional location inside the single cell by obtaining specific spectral features. Au NPs modified by 4-mercaptobenzoic acid (MBA) bearing its -COOH surface functional group were used to conjugate transferrin (Tf) protein using the 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) reaction. The protein conjugation reaction on Au surfaces was examined by means of color change, absorption spectroscopy, and SERS. Our results demonstrate that DFM techniques combined with SERS may have great potential for monitoring biological processes with protein conjugation at the single-cell level.
authors: Department of Chemistry, Soongsil University, Seoul 156-743, Korea.
authors: Jin-Ho
authors: JH
authors: Park
authors: Jin
authors: J
authors: Park
authors: Uuriintuya
authors: U
authors: Dembereldorj
authors: Keunchang
authors: K
authors: Cho
authors: Kangtaek
authors: K
authors: Lee
authors: Sung Ik
authors: SI
authors: Yang
authors: So Yeong
authors: SY
authors: Lee
authors: Sang-Woo
authors: SW
authors: Joo
mesh_terms: D001565:Benzoates / Q000737:chemistry; D002460:Cell Line; D003593:Cytoplasm / Q000648:ultrastructure*; D004867:Equipment Design; D006046:Gold / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000032:analysis* / Q000737:chemistry; D008853:Microscopy / Q000295:instrumentation; D008958:Models, Molecular; D059010:Single-Cell Analysis / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014168:Transferrin / Q000032:analysis* / Q000737:chemistry
chemical_list: D001565:Benzoates; D013438:Sulfhydryl Compounds; D014168:Transferrin; C013594:4-mercaptobenzoate; D006046:Gold