title: Exploring the Schistosoma mansoni adult male transcriptome using RNA-seq.
abstract: Schistosoma mansoni is one of the agents of schistosomiasis, a chronic and debilitating disease. Here we present a transcriptome-wide characterization of adult S. mansoni males by high-throughput RNA-sequencing. We obtained 1,620,432 high-quality ESTs from a directional strand-specific cDNA library, resulting in a 26% higher coverage of genome bases than that of the public ESTs available at NCBI. With a 15×-deep coverage of transcribed genomic regions, our data were able to (i) confirm for the first time 990 predictions without previous evidence of transcription; (ii) correct gene predictions; (iii) discover 989 and 1196 RNA-seq contigs that map to intergenic and intronic genomic regions, respectively, where no gene had been predicted before. These contigs could represent new protein-coding genes or non-coding RNAs (ncRNAs). Interestingly, we identified 11 novel Micro-exon genes (MEGs). These data reveal new features of the S. mansoni transcriptional landscape and significantly advance our understanding of the parasite transcriptome.
authors: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.
authors: Giulliana Tessarin
authors: GT
authors: Almeida
authors: Murilo Sena
authors: MS
authors: Amaral
authors: Felipe Cesar Ferrarezi
authors: FC
authors: Beckedorff
authors: João Paulo
authors: JP
authors: Kitajima
authors: Ricardo
authors: R
authors: DeMarco
authors: Sergio
authors: S
authors: Verjovski-Almeida
mesh_terms: D000818:Animals; D020451:Contig Mapping; D005091:Exons; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D017238:Genes, Helminth / Q000235:genetics*; D050259:Genes, Mitochondrial; D049751:Genome, Helminth; D059014:High-Throughput Nucleotide Sequencing; D008297:Male; D008969:Molecular Sequence Data; D018510:RNA, Helminth / Q000737:chemistry*; D012549:Schistosoma japonicum / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics*; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D018510:RNA, Helminth