title: Para-hydrogen induced polarization of amino acids, peptides and deuterium-hydrogen gas.
abstract: Signal Amplification by Reversible-Exchange (SABRE) is a method of hyperpolarizing substrates by polarization transfer from para-hydrogen without hydrogenation. Here, we demonstrate that this method can be applied to hyperpolarize small amounts of all proteinogenic amino acids and some chosen peptides down to the nanomole regime and can be detected in a single scan in low-magnetic fields down to 0.25 mT (10 kHz proton frequency). An outstanding feature is that depending on the chemical state of the used catalyst and the investigated amino acid or peptide, hyperpolarized hydrogen-deuterium gas is formed, which was detected with (1)H and (2)H NMR spectroscopy at low magnetic fields of B(0) = 3.9 mT (166 kHz proton frequency) and 3.2 mT (20 kHz deuterium frequency).
authors: Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany. stgloeggler@gmx.de
authors: Stefan
authors: S
authors: Glöggler
authors: Rafael
authors: R
authors: Müller
authors: Johannes
authors: J
authors: Colell
authors: Meike
authors: M
authors: Emondts
authors: Martin
authors: M
authors: Dabrowski
authors: Bernhard
authors: B
authors: Blümich
authors: Stephan
authors: S
authors: Appelt
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry; D005740:Gases / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D006865:Hydrogenation; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D005740:Gases; D010455:Peptides; D006859:Hydrogen; D003903:Deuterium