title: Gene and protein expression analysis of mesenchymal stem cells derived from rat adipose tissue and bone marrow.
abstract: BACKGROUND
  Mesenchymal stem cells (MSC) are multipotent and reside in bone marrow (BM), adipose tissue and many other tissues. However, the molecular foundations underlying the differences in proliferation, differentiation potential and paracrine effects between adipose tissue-derived MSC (ASC) and BM-derived MSC (BM-MSC) are not well-known. Therefore, we investigated differences in the gene and secretory protein expressions of the 2 types of MSC.
  
  
  RESULTS
  ASC and BM-MSC were obtained from subcutaneous adipose tissue and BM of adult Lewis rats. ASC proliferated as rapidly as BM-MSC, and had expanded 200-fold in approximately 2 weeks. On microarray analysis of 31,099 genes, 571 (1.8%) were more highly (>3-fold) expressed in ASC, and a number of these genes were associated with mitosis and immune response. On the other hand, 571 genes (1.8%) were more highly expressed in BM-MSC, and some of these genes were associated with organ development and morphogenesis. In secretory protein analysis, ASC secreted significantly larger amounts of growth factor and inflammatory cytokines, such as vascular endothelial growth factor, hepatocyte growth factor and interleukin 6, whereas BM-MSC secreted significantly larger amounts of stromal-derived factor-1α.
  
  
  CONCLUSIONS
  There are significant differences between ASC and BM-MSC in the cytokine secretome, which may provide clues to the molecule mechanisms associated with tissue regeneration and alternative cell sources.
authors: Division of Cardiovascular Medicine, Kanazawa University Graduate School of Medicine, Japan.
authors: Chiaki
authors: C
authors: Nakanishi
authors: Noritoshi
authors: N
authors: Nagaya
authors: Shunsuke
authors: S
authors: Ohnishi
authors: Kenichi
authors: K
authors: Yamahara
authors: Shu
authors: S
authors: Takabatake
authors: Tetsuo
authors: T
authors: Konno
authors: Kenshi
authors: K
authors: Hayashi
authors: Masa-Aki
authors: MA
authors: Kawashiri
authors: Toshinari
authors: T
authors: Tsubokawa
authors: Masakazu
authors: M
authors: Yamagishi
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D049109:Cell Proliferation; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011917:Rats, Inbred Lew; D050151:Subcutaneous Fat / Q000166:cytology / Q000378:metabolism*