title: Tetraphenylethylene-based glycoconjugate as a fluorescence "turn-on" sensor for cholera toxin.
abstract: Tetraphenylethylene (TPE)-based glycoconjugates were easily synthesized by copper(I)-catalyzed "click reactions" between propargyl-attached TPE and azido-functionalized sugars. The TPE compound bearing lactosyl moieties (Lac-TPE) was found to be a fluorescence "turn-on" sensor for cholera toxin by virtue of aggregation-induced emission characteristics of the TPE motif owing to the specific interaction of lactose with the cholera toxin B subunit, whilst a cellobiose-functionalized TPE derivative did not show any response to the toxin. Therefore, Lac-TPE shows promising applications in the detection of cholera toxin, as well as in the investigation of carbohydrate-protein interaction.
authors: National Center for Nanoscience and Technology, Beijing 100190, China.
authors: Xin-Ming
authors: XM
authors: Hu
authors: Qi
authors: Q
authors: Chen
authors: Jin-Xiang
authors: JX
authors: Wang
authors: Qian-Yi
authors: QY
authors: Cheng
authors: Chao-Guo
authors: CG
authors: Yan
authors: Jie
authors: J
authors: Cao
authors: Yu-Jian
authors: YJ
authors: He
authors: Bao-Hang
authors: BH
authors: Han
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002772:Cholera Toxin / Q000032:analysis*; D018360:Crystallography, X-Ray; D005030:Ethylenes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D007785:Lactose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013267:Stilbenes / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005030:Ethylenes; D005456:Fluorescent Dyes; D006001:Glycoconjugates; D013267:Stilbenes; D002772:Cholera Toxin; D007785:Lactose