title: Quantitative analysis of the relative transcript levels of four chlorophenol reductive dehalogenase genes in Desulfitobacterium hafniense PCP-1 exposed to chlorophenols.
abstract: Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as was cprA5, but concomitantly with the appearance of 2,4-dichlorophenol (DCP). In 3,5-DCP-amended cultures, only cprA5 was upregulated. In pentachlorophenol-amended cultures grown for 12 h, cprA2 and cprA3 were upregulated but not cprA5. cprA4 was not upregulated significantly in cultures containing any tested chlorophenols.
authors: INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, Québec, Canada.
authors: Ariane
authors: A
authors: Bisaillon
authors: Réjean
authors: R
authors: Beaudet
authors: François
authors: F
authors: Lépine
authors: Richard
authors: R
authors: Villemur
mesh_terms: D002733:Chlorophenols / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D045858:Desulfitobacterium / Q000187:drug effects* / Q000201:enzymology*; D020869:Gene Expression Profiling; D010088:Oxidoreductases / Q000096:biosynthesis*
chemical_list: D002733:Chlorophenols; D003470:Culture Media; D010088:Oxidoreductases