title: Methyl jasmonate induces ATP biosynthesis deficiency and accumulation of proteins related to secondary metabolism in Catharanthus roseus (L.) G. hairy roots.
abstract: Jasmonates are specific signal molecules in plants that are involved in a diverse set of physiological and developmental processes. However, methyl jasmonate (MeJA) has been shown to have a negative effect on root growth and, so far, the biochemical mechanism for this is unknown. Using Catharanthus roseus hairy roots, we were able to observe the effect of MeJA on growth inhibition, cell disorganization and cell death of the root cap. Hairy roots treated with MeJA induced the perturbation of mitochondrial membrane integrity and a diminution in ATP biosynthesis. Furthermore, several proteins were identified that were involved in energy and secondary metabolism; the changes in accumulation of these proteins were observed with 100 μM MeJA. In conclusion, our results suggest that a switch of the metabolic fate of hairy roots in response to MeJA could cause an increase in the accumulation of secondary metabolites. This is likely to have important consequences in the production of specific alkaloids important for the pharmaceutical industry.
authors: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Col. Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, México.
authors: Eliel
authors: E
authors: Ruiz-May
authors: Clelia
authors: C
authors: De-la-Peña
authors: Rosa M
authors: RM
authors: Galaz-Ávalos
authors: Zhentian
authors: Z
authors: Lei
authors: Bonnie S
authors: BS
authors: Watson
authors: Lloyd W
authors: LW
authors: Sumner
authors: Víctor M
authors: VM
authors: Loyola-Vargas
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects; D030008:Catharanthus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D018518:Plant Root Cap / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D020543:Proteome / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects
chemical_list: D000085:Acetates; D000596:Amino Acids; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D020543:Proteome; D000255:Adenosine Triphosphate; C072239:methyl jasmonate