title: High mobility group nucleosomal binding domain 2 protein protects bladder epithelial cells from Klebsiella pneumoniae invasion.
abstract: Due to the predominance of multiple-antibiotic-resistant Klebsiella pneumoniae strains, the search for new approaches for the prevention of K. pneumoniae infections is now under intensive investigation. The objective of the present study was to investigate the effects of high mobility group nucleosomal binding domain 2 (HMGN2) protein, which acts on the bladder epithelial cells T 24, on the invasion of K. pneumoniae 03183 and explore its possible mechanisms. Pretreatment with HMGN2 significantly reduced K. pneumoniae 03183 uptake by T 24 cells. In T 24 cells, there were no detectable cytotoxic effects of HMGN2 at any concentration between 32 and 256 µg/ml after 2 h incubation. HMGN2 exhibited no appreciable antibacterial activity against K. pneumoniae 03183. Fluorescence microscopy and flow cytometry analysis revealed that HMGN2 blocked K. pneumoniae 03183-induced actin polymerization. K. pneumoniae 03183-induced phosphorylation of extracellular signal-regulated kinase (ERK) and cofilin were prevented by pretreatment with HMGN2. These results indicated that pretreatment with HMGN2 inhibited cofilin phosphorylation and then induced actin disruption which may block ERK phosphorylation. These changes led to inhibition of K. pneumoniae 03183 invasion of T 24 bladder epithelial cells.
authors: Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University, Chengdu, Sichuan Province 610041, China.
authors: Yue
authors: Y
authors: Cao
authors: Guixia
authors: G
authors: Wu
authors: Bo
authors: B
authors: Fan
authors: Fengjin
authors: F
authors: Zheng
authors: Xiang
authors: X
authors: Gao
authors: Na
authors: N
authors: Liu
authors: Xiaokang
authors: X
authors: Liu
authors: Ning
authors: N
authors: Huang
mesh_terms: D000199:Actins / Q000378:metabolism; D015153:Blotting, Western; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology*; D005434:Flow Cytometry; D024242:HMGN2 Protein / Q000502:physiology*; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction; D001743:Urinary Bladder / Q000166:cytology / Q000382:microbiology*
chemical_list: D000199:Actins; D024242:HMGN2 Protein; D020928:Mitogen-Activated Protein Kinases