title: The auxin signalling network translates dynamic input into robust patterning at the shoot apex.
abstract: The plant hormone auxin is thought to provide positional information for patterning during development. It is still unclear, however, precisely how auxin is distributed across tissues and how the hormone is sensed in space and time. The control of gene expression in response to auxin involves a complex network of over 50 potentially interacting transcriptional activators and repressors, the auxin response factors (ARFs) and Aux/IAAs. Here, we perform a large-scale analysis of the Aux/IAA-ARF pathway in the shoot apex of Arabidopsis, where dynamic auxin-based patterning controls organogenesis. A comprehensive expression map and full interactome uncovered an unexpectedly simple distribution and structure of this pathway in the shoot apex. A mathematical model of the Aux/IAA-ARF network predicted a strong buffering capacity along with spatial differences in auxin sensitivity. We then tested and confirmed these predictions using a novel auxin signalling sensor that reports input into the signalling pathway, in conjunction with the published DR5 transcriptional output reporter. Our results provide evidence that the auxin signalling network is essential to create robust patterns at the shoot apex.
authors: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, Lyon, France. teva.vernoux@ens-lyon.fr
authors: Teva
authors: T
authors: Vernoux
authors: Géraldine
authors: G
authors: Brunoud
authors: Etienne
authors: E
authors: Farcot
authors: Valérie
authors: V
authors: Morin
authors: Hilde
authors: H
authors: Van den Daele
authors: Jonathan
authors: J
authors: Legrand
authors: Marina
authors: M
authors: Oliva
authors: Pradeep
authors: P
authors: Das
authors: Antoine
authors: A
authors: Larrieu
authors: Darren
authors: D
authors: Wells
authors: Yann
authors: Y
authors: Guédon
authors: Lynne
authors: L
authors: Armitage
authors: Franck
authors: F
authors: Picard
authors: Soazig
authors: S
authors: Guyomarc'h
authors: Coralie
authors: C
authors: Cellier
authors: Geraint
authors: G
authors: Parry
authors: Rachil
authors: R
authors: Koumproglou
authors: John H
authors: JH
authors: Doonan
authors: Mark
authors: M
authors: Estelle
authors: Christophe
authors: C
authors: Godin
authors: Stefan
authors: S
authors: Kepinski
authors: Malcolm
authors: M
authors: Bennett
authors: Lieven
authors: L
authors: De Veylder
authors: Jan
authors: J
authors: Traas
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D017404:In Situ Hybridization, Fluorescence; D007210:Indoleacetic Acids / Q000494:pharmacology*; D018519:Meristem / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008962:Models, Theoretical; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D038081:Organogenesis; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D030821:Plants, Genetically Modified; D015398:Signal Transduction / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C491592:IAA1 protein, Arabidopsis; D007210:Indoleacetic Acids; D009687:Nuclear Proteins; D010937:Plant Growth Regulators