title: Intensified extraction of ionized natural products by ion pair centrifugal partition extraction.
abstract: The potential of centrifugal partition extraction (CPE) combined with the ion-pair (IP) extraction mode to simultaneously extract and purify natural ionized saponins from licorice is presented in this work. The design of the instrument, a new laboratory-scale Fast Centrifugal Partition Extractor (FCPE300(®)), has evolved from centrifugal partition chromatography (CPC) columns, but with less cells of larger volume. Some hydrodynamic characteristics of the FCPE300(®) were highlighted by investigating the retention of the stationary phase under different flow rate conditions and for different biphasic solvent systems. A method based on the ion-pair extraction mode was developed to extract glycyrrhizin (GL), a biologically active ionic saponin naturally present in licorice (Glycyrrhiza glabra L., Fabaceae) roots. The extraction of GL was performed at a flow rate of 20 mL/min in the descending mode by using the biphasic solvent system ethyl acetate/n-butanol/water in the proportions 3/2/5 (v/v/v). Trioctylmethylammonium with chloride as a counter-ion (Al336(®)) was used as the anion extractant in the organic stationary phase and iodide, with potassium as counter-ion, was used as the displacer in the aqueous mobile phase. From 20 g of a crude extract of licorice roots, 2.2g of GL were recovered after 70 min, for a total process duration of 90 min. The combination of the centrifugal partition extractor with the ion-pair extraction mode (IP-CPE) offers promising perspectives for industrial applications in the field of natural product isolation or for the fractionation of natural complex mixtures.
authors: UMR CNRS 6229, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de la Housse, BP 1039, 51687 Reims, Cedex 2, France.
authors: Mahmoud
authors: M
authors: Hamzaoui
authors: Jane
authors: J
authors: Hubert
authors: Jamila
authors: J
authors: Hadj-Salem
authors: Bernard
authors: B
authors: Richard
authors: Dominique
authors: D
authors: Harakat
authors: Luc
authors: L
authors: Marchal
authors: Alain
authors: A
authors: Foucault
authors: Catherine
authors: C
authors: Lavaud
authors: Jean-Hugues
authors: JH
authors: Renault
mesh_terms: D002498:Centrifugation / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D006035:Glycyrrhiza / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification*; D012503:Saponins / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D012503:Saponins