title: Claudin-5 expression in in vitro models of the blood-brain barrier.
abstract: Claudins are transmembrane proteins that form the backbone of the tight junctions (TJs) at the blood-brain barrier (BBB). TJs are cellular structures that physically obstruct the inter-endothelial space and restrict the paracellular diffusion of blood-borne substances from the peripheral circulation into the CNS. TJs are also dynamic structures that rapidly respond to external signals that produce changes in BBB permeability. We focus here on the biochemical and immunohistochemical properties of claudin-5 as expressed in three in vitro models of the BBB, and show that the contact co-culture of endothelial cells with glial cells significantly increases claudin-5 expression.
authors: Itzik
authors: I
authors: Cooper
authors: Katayun
authors: K
authors: Cohen-Kashi-Malina
authors: Vivian I
authors: VI
authors: Teichberg
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D015153:Blotting, Western / Q000379:methods; D018929:Cell Culture Techniques / Q000379:methods*; D057167:Claudins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism* / Q000502:physiology; D007150:Immunohistochemistry / Q000379:methods; D009457:Neuroglia / Q000378:metabolism* / Q000502:physiology; D013552:Swine; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D057167:Claudins