title: CRES-T, an effective gene silencing system utilizing chimeric repressors.
abstract: Chimeric REpressor gene Silencing Technology (CRES-T) is a useful tool for functional analysis of plant transcription factors. In this system, a chimeric repressor that is produced by fusion of a transcription factor to the plant-specific EAR-motif repression domain (SRDX) suppresses target genes of a transcription factor dominantly over the activity of endogenous and functionally redundant transcription factors. As a result, the transgenic plants that express a chimeric repressor exhibit phenotypes similar to loss-of-function of the alleles of the gene encoding the transcription factor. This system is simple and effective and can be used as a powerful tool not only for functional analysis of redundant transcription factors but also for the manipulation of plant traits by active suppression of the gene expression. Strategies for construction of the chimeric repressors and their expression in transgenic plants are described. Transient effector-reporter assays for functional analysis of transcription factors and detection of protein-protein interactions using the trans-repressive activity of SRDX repression domain are also described.
authors: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. nobutaka.mitsuda@aist.go.jp
authors: Nobutaka
authors: N
authors: Mitsuda
authors: Kyoko
authors: K
authors: Matsui
authors: Miho
authors: M
authors: Ikeda
authors: Masaru
authors: M
authors: Nakata
authors: Yoshimi
authors: Y
authors: Oshima
authors: Yukari
authors: Y
authors: Nagatoshi
authors: Masaru
authors: M
authors: Ohme-Takagi
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D020868:Gene Silencing; D050506:Mutant Chimeric Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050506:Mutant Chimeric Proteins; D012097:Repressor Proteins; D014157:Transcription Factors