title: Increased oxidative stress and gut ischemia caused by prolonged pneumoperitoneum in patients undergoing robot-assisted laparoscopic radical prostatectomy.
abstract: OBJECTIVE
  To investigate changes in plasma oxidative stress markers associated with prolonged pneumoperitoneum during robot-assisted laparoscopic radical prostatectomy (RALP).
  
  
  METHODS
  In all, patients of ASA Physical Status II-III who intended to undergo RALP were enrolled in the study. Arterial plasma levels of malondialdehyde (MDA) and intramucosal pH were measured 1 minute before and at 1 hour, 2 hours, 3 hours, and 4 hours after the introduction of pneumoperitoneum at an insufflation pressure of 15 mmHg; likewise, they were again measured every 10 minutes after deflation for 60 minutes, at 2 hours and 12 hours after deflation.
  
  
  RESULTS
  The mean duration of pneumoperitoneum was around 4 hours. After induction of pneumoperitoneum, the MDA concentrations were significantly elevated at various time points as compared with the preinsufflation value. Significant increase of MDA value was noted also 30 minutes after deflation as compared with the pre-deflation levels. The intramucosal pH value decreased significantly after CO(2) insufflation compared with the preinsufflation values. It also increased significantly 2 hours after CO(2) deflation as compared with the pre-deflation values.
  
  
  CONCLUSIONS
  A prolonged pneumoperitoneum in RALP results in decreased splanchnic blood flow. Pneumoperitoneum itself produces oxidative stress, and ischemia-reperfusion model after deflation of pneumoperitoneum produces more oxidative stress.
authors: Department of Anesthesiology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, ROC.
authors: Chiao-Fen
authors: CF
authors: Luo
authors: Yung-Fong
authors: YF
authors: Tsai
authors: Chia-Hung
authors: CH
authors: Chang
authors: Chun-Te
authors: CT
authors: Wu
authors: Huang-Ping
authors: HP
authors: Yu
mesh_terms: D000368:Aged; D005745:Gastric Acidity Determination; D041981:Gastrointestinal Tract / Q000098:blood supply*; D006801:Humans; D007511:Ischemia / Q000209:etiology*; D010535:Laparoscopy; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011468:Prostatectomy; D012371:Robotics / Q000379:methods*
chemical_list: D008315:Malondialdehyde