title: Identification of specific nuclear structural protein alterations in human breast cancer.
abstract: Breast cancer is the most commonly diagnosed type of cancer and a major cause of death in women. Reliable biomarkers are urgently needed to improve early detection or to provide evidence of the prognosis for each individual patient through expression levels in tumor tissue or body fluids. This proteomic analysis focused on the nuclear structure of human breast cancer tissue, which has been shown to be a promising tool for cancer biomarker development. The nuclear matrix composition of human breast cancer (n = 14), benign controls (n = 2), and healthy controls (n = 2) was analyzed by high-resolution two-dimensional gel electrophoresis and mass spectrometry. Validation studies were performed in an individual sample set consisting of additional breast cancer tissues (n = 3) and additional healthy control tissues (n = 2) by one-dimensional immunoblot. In this setting, we identified five proteins that were upregulated in human breast cancer tissue, but absent in the healthy and benign controls (P < 0.001). These spots were also present in the investigated human breast cancer cell lines, but absent in the MCF10a cell line, which represents normal human epithelial breast cells. Two of the breast cancer-specific proteins have been confirmed to be calponin h2 and calmodulin-like protein 5 by one-dimensional immunoblot. This is the first study demonstrating the expression of both proteins in human breast cancer tissue. Further studies are required to investigate the potential role of these proteins as biomarkers for early diagnosis or prognosis in human breast cancer.
authors: Department of Obstetrics and Gynecology, Centre for Integrated Oncology, University of Bonn, Bonn, Germany.
authors: Manuel
authors: M
authors: Debald
authors: Sebastian
authors: S
authors: Franken
authors: Lukas Carl
authors: LC
authors: Heukamp
authors: Andrea
authors: A
authors: Linke
authors: Matthias
authors: M
authors: Wolfgarten
authors: Klaus-Jürgen
authors: KJ
authors: Walgenbach
authors: Michael
authors: M
authors: Braun
authors: Christian
authors: C
authors: Rudlowski
authors: Volkmar
authors: V
authors: Gieselmann
authors: Walther
authors: W
authors: Kuhn
authors: Gunther
authors: G
authors: Hartmann
authors: Gisela
authors: G
authors: Walgenbach-Brünagel
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D009363:Neoplasm Proteins