title: Maesopsin 4-O-beta-D-glucoside, a natural compound isolated from the leaves of Artocarpus tonkinensis, inhibits proliferation and up-regulates HMOX1, SRXN1 and BCAS3 in acute myeloid leukemia.
abstract: The leaves of Artocarpus tonkinensis are used in Vietnamese traditional medicine for treatment of arthritis, and the compound maesopsin 4-O-β-D-glucoside (TAT-2), isolated from them, inhibits the proliferation of activated T cells. Our goal was to test the anti-proliferative activity of TAT-2 on the T-cell leukemia, Jurkat, and on the acute myeloid leukemia, OCI-AML. TAT-2 inhibited the growth of OCI-AML (and additional acute myeloid leukemia cells) but not Jurkat cells. Growth inhibition was shown to be due to inhibition of proliferation rather than increase in cell death. Analysis of cytokine release showed that TAT-2 stimulated the release of TGF-β, yet TGF-β neutralization did not reverse the maesopsin-dependent effect. Gene expression profiling determined that maesopsin modulated 19 identifiable genes. Transcription factor CP2 was the gene most significantly modulated. Real-time PCR validated that up-regulation of sulphiredoxin 1 homolog (SRXN1), hemeoxygenase 1 (HMOX1), and breast carcinoma amplified sequence 3 (BCAS3) were consistently modulated.
authors: Section of Pharmacology, Toxicology and Chemotherapy, Department of Clinical and Experimental Medicine, University of Perugia, Via del Giochetto, Perugia, Italy.
authors: N
authors: N
authors: Pozzesi
authors: S
authors: S
authors: Pierangeli
authors: Carmine
authors: C
authors: Vacca
authors: L
authors: L
authors: Falchi
authors: V
authors: V
authors: Pettorossi
authors: M P
authors: MP
authors: Martelli
authors: T T
authors: TT
authors: Thuy
authors: P T
authors: PT
authors: Ninh
authors: A M
authors: AM
authors: Liberati
authors: C
authors: C
authors: Riccardi
authors: T V
authors: TV
authors: Sung
authors: D V
authors: DV
authors: Delfino
mesh_terms: D031622:Artocarpus / Q000737:chemistry; D001572:Benzofurans / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D020869:Gene Expression Profiling / Q000379:methods; D005960:Glucosides / Q000494:pharmacology*; D018922:HL-60 Cells; D051547:Heme Oxygenase-1 / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D019169:Jurkat Cells; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015458:Leukemia, T-Cell / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000096:biosynthesis / Q000235:genetics*; D013601:T-Lymphocytes / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism; D020298:U937 Cells; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C470217:BCAS3 protein, human; D001572:Benzofurans; D004268:DNA-Binding Proteins; D005960:Glucosides; D009363:Neoplasm Proteins; C501906:TFCP2 protein, human; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C485327:maesopsin 4-O-glucoside; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D050862:Oxidoreductases Acting on Sulfur Group Donors; C504024:SRXN1 protein, human