title: Highly efficient enzymatic synthesis of 3'-deoxyapionucleic acid (apioNA) having the four natural nucleobases.
abstract: The synthesis of the 3'-deoxyapionucleoside 3''-triphosphates (apioNTPs) having the four natural nucleobases and their enzymatic incorporation into a DNA-DNA primer-template have been tried. Therminator DNA polymerase was shown to incorporate these apioNTPs effectively giving 43mer DNA-apioNA chimera.
authors: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido 060-0812, Japan.
authors: Mayumi
authors: M
authors: Kataoka
authors: Yasuo
authors: Y
authors: Kouda
authors: Kousuke
authors: K
authors: Sato
authors: Noriaki
authors: N
authors: Minakawa
authors: Akira
authors: A
authors: Matsuda
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D007700:Kinetics; D009841:Oligonucleotides / Q000096:biosynthesis*
chemical_list: D017931:DNA Primers; D009841:Oligonucleotides; D004259:DNA-Directed DNA Polymerase