title: ¹H, ¹³C and ¹⁵N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72.
abstract: Ssu72 helps regulate transcription and co-transcriptional mRNA processing by dephosphorylating serine residues at the 5th position in the heptad repeats of the C-terminal domain of RNA polymerase II. Here we use multidimensional, multinuclear NMR experiments to assign the backbone and side-chain resonances of the 23 kDa Ssu72 from Drosophila melanogaster in the phosphate-bound state, and use NMR titrations to examine the phosphate-binding properties of three active site mutants.
authors: Department of Biochemistry, Duke University Medical Center, 270 Sands Building, Research Drive, Durham, NC 27710, USA.
authors: Jon W
authors: JW
authors: Werner-Allen
authors: Pei
authors: P
authors: Zhou
mesh_terms: D000818:Animals; D001665:Binding Sites; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000235:genetics; D019906:Nuclear Magnetic Resonance, Biomolecular; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029721:Drosophila Proteins; D017027:Protein Tyrosine Phosphatases; C557721:Ssu72 protein, Drosophila