title: Protein labeling enhances aptamer selection by methods of kinetic capillary electrophoresis.
abstract: Methods of kinetic capillary electrophoresis (KCE) facilitate highly efficient selection of DNA aptamers for protein targets. The inability to detect native proteins at low concentrations in capillary electrophoresis creates, however, a significant obstacle for many important protein targets. Here we suggest that protein labeling with new Chromeo dyes can help to overcome this obstacle. By labeling a number of proteins with Chromeo P503, we show that the labeling procedure enables accurate detection of proteins in CE without significantly affecting their electrophoretic mobility or their ability to bind DNA. Moreover, Chromeo P503 does not appear to label the amino-groups of buffer components to a significant extent, making the labeling procedure compatible with a large number of selection and run buffers. Fluorescent labeling of protein targets with Chromeo dyes empowers selection of aptamers by KCE methods and promises to increase the rate at which aptamers for new targets are being developed and introduced in various applications.
authors: Department of Chemistry, York University, Toronto, Ontario, Canada.
authors: Stephanie
authors: S
authors: de Jong
authors: Sergey N
authors: SN
authors: Krylov
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism*; D002021:Buffers; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011166:Porphyrins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D002021:Buffers; D005456:Fluorescent Dyes; D011166:Porphyrins; D011506:Proteins; C006101:P503