title: CD34+ cell enumeration by flow cytometry: a comparison of systems and methodologies.
abstract: BACKGROUND
  An increasing number of medical centers can collect bone marrow, peripheral blood, or umbilical cord stem cells. Pathology laboratories should accommodate this trend, but investment in additional equipment may be impractical.
  
  
  OBJECTIVE
  To compare CD34(+) cell counting results by using 2 widely available flow cytometry systems, with and without the use of a separate hematology analyzer (ie, single-platform versus dual-platform methodologies).
  
  
  METHODS
  Whole blood and peripheral blood stem cell (PBSC) samples were analyzed from 13 healthy allogeneic PBSC donors and 46 autologous PBSC donors with various malignancies. The Cytomics FC500 (Beckman Coulter, Fullerton, California) was compared with the FACSCalibur (BD Biosciences, San Jose, California). Dual-platform CD34(+) cell counting incorporated data from a KX-21 hematology analyzer (Sysmex, Kobe, Japan).
  
  
  RESULTS
  Subtle differences in CD34(+) cell counting between 2 systems and 2 methods did not achieve statistical significance.
  
  
  CONCLUSIONS
  Different systems and methods for CD34(+) cell enumeration, properly validated, can support care for patients undergoing transplants and provide meaningful data for multicenter studies or meta-analyses.
authors: Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima City, Fukushima, Japan.
authors: Alain
authors: A
authors: Ngoma
authors: Shunnichi
authors: S
authors: Saito
authors: Hitoshi
authors: H
authors: Ohto
authors: Kazuhiko
authors: K
authors: Ikeda
authors: Hiroyasu
authors: H
authors: Yasuda
authors: Kinuyo
authors: K
authors: Kawabata
authors: Takahiro
authors: T
authors: Kanno
authors: Atsushi
authors: A
authors: Kikuta
authors: Kazuhiro
authors: K
authors: Mochizuki
authors: Kenneth E
authors: KE
authors: Nollet
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis* / Q000276:immunology*; D002452:Cell Count / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000166:cytology / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged
chemical_list: D018952:Antigens, CD34