title: Dimethylformamide as a cryoprotectant for canine semen diluted and frozen in ACP-106C.
abstract: The objective was to assess the effect of adding various concentrations of dimethylformamide on characteristics of canine semen diluted in powdered coconut water (ACP-106C; ACP Biotecnologia, Fortaleza, CE, Brazil) and frozen at -196°C. Fifteen ejaculates were collected by manual stimulation from five adult Boxer dogs. The sperm-rich fraction was diluted in ACP-106C (ACP Biotecnologia) containing 10% egg yolk and divided into four aliquots. The cryoprotectants used for each aliquot were 6% glycerol (control group; CG) or 2%, 4%, or 6% dimethylformamide (DF2, DF4, and DF6, respectively). After thawing, total motility (mean ± SEM) for CG (58.4 ± 24.6) was higher (P < 0.05) than that of the other groups (2% dimethylformamide, 24.4 ± 12.3; 4% dimethylformamide, 26.5 ± 16.1; and 6% dimethylformamide, 21.7 ± 17.9). Furthermore, there was a greater percentage of fast, average, and slow moving sperm (assessed with computer-aided semen analysis; CASA) in CG in comparison with the other three groups. Therefore, based on concentrations tested in this study, dimethylformamide, together with ACP-106C (ACP Biotecnologia) and 10% egg yolk as a diluent, yielded unsatisfactory in vitro results for freezing canine semen.
authors: Carnivore Reproduction Laboratory, Veterinary College, State University of Ceará, Fortaleza, CE, Brazil.
authors: A C
authors: AC
authors: Mota Filho
authors: C H A
authors: CH
authors: Teles
authors: R P
authors: RP
authors: Jucá
authors: J F S
authors: JF
authors: Cardoso
authors: D C
authors: DC
authors: Uchoa
authors: C C
authors: CC
authors: Campello
authors: A R
authors: AR
authors: Silva
authors: L D M
authors: LD
authors: Silva
mesh_terms: D000818:Animals; D003059:Cocos / Q000737:chemistry; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004126:Dimethylformamide / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D008297:Male; D028321:Plant Preparations / Q000008:administration & dosage / Q000494:pharmacology*; D012661:Semen; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*
chemical_list: C570992:ACP-106C; D003451:Cryoprotective Agents; D028321:Plant Preparations; D004126:Dimethylformamide