title: Triterpenoid saponins from the seeds of Caragana microphylla.
abstract: Two new triterpenoid saponins, namely caraganoside C (1) and caraganoside D (2), were isolated from the seeds of Caragana microphylla. Their structures were elucidated on the basis of spectroscopic analyses, including homo- and hetero-nuclear correlation NMR experiments (COSY, HSQC and HMBC). Both 1 and 2 exhibited moderate inhibitory activity against NO production in LPS-stimulated RAW264.7 cells with IC(50) values of 26.4 μM and 32.2 μM, respectively. In addition, 1 showed weak cytotoxicity against MCF-7, HL-60, HCT116, and A549 cell lines.
authors: Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, China.
authors: Gui-Lin
authors: GL
authors: Jin
authors: Cheng-Jian
authors: CJ
authors: Zheng
authors: Wen-Bo
authors: WB
authors: Xin
authors: Zhu-Jun
authors: ZJ
authors: Mao
authors: Pei-Xin
authors: PX
authors: Sun
authors: Zhi-Xin
authors: ZX
authors: Zeng
authors: Lu-Ping
authors: LP
authors: Qin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D032602:Caragana / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D008070:Lipopolysaccharides / Q000633:toxicity; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D012503:Saponins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds; D014315:Triterpenes / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D008070:Lipopolysaccharides; D012503:Saponins; D014315:Triterpenes; C562030:caraganoside C; C562031:caraganoside D; D009569:Nitric Oxide; D004317:Doxorubicin