title: Investigation of temperature-responsivity and aqueous chromatographic characteristics of a thermo-responsive monolithic column.
abstract: A thermo-responsive and macroporous monolithic cryogel was directly prepared by in situ free-radical redox cryo-polymerization in a stainless steel chromatographic column (100 mm × 4.6mm i.d.) using N-isopropylacrylamide (NIPAAm) as functional monomer and PEG-20,000 as porogen at -12°C. The internal morphology of resulting monolithic cryogel was estimated by scanning electron microscopy (SEM). Based on that, a submicron skeleton structure was observed. Besides, the gravimetrically determined rates of swelling/deswelling for thermo-responsive monolithic cryogel were much higher than that of hydrogel adopting the same component proportion via conventional method (25°C for 24h). Simultaneously, a temperature-dependent resolution of steroids was also achieved using only water as a mobile phase. The theoretical plate number of every analyte was more than 2000.
authors: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China. lhy1610@126.com
authors: Haiyan
authors: H
authors: Liu
authors: Mingquan
authors: M
authors: Liu
authors: Ligai
authors: L
authors: Bai
authors: Suying
authors: S
authors: Sun
authors: Yankun
authors: Y
authors: Liu
authors: Gengliang
authors: G
authors: Yang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000180:Acrylic Resins; D002845:Chromatography / Q000379:methods*; D059985:Cryogels; D020100:Hydrogels / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D013193:Stainless Steel / Q000737:chemistry; D013256:Steroids / Q000302:isolation & purification; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D059985:Cryogels; D020100:Hydrogels; D011108:Polymers; D013256:Steroids; D014867:Water; D013193:Stainless Steel; C052970:poly-N-isopropylacrylamide; D011092:Polyethylene Glycols