title: Effects of Human Adipose-derived Stem Cells on Cutaneous Wound Healing in Nude Mice.
abstract: BACKGROUND
  Despite numerous treatments available for deteriorated cutaneous wound healing such as a diabetic foot, there is still the need for more effective therapy. Adipose-derived stem cells (ASCs) are mesenchymal stem cells, which are self-renewing and multipotent. Mesenchymal stem cells have the potential for tissue repair and regeneration.
  
  
  OBJECTIVE
  To investigate the effects of human ASCs on the healing of cutaneous wounds in nude mice.
  
  
  METHODS
  15-mm round full-thickness skin defects were generated on the back of BALB/c nude mice. The mice were divided into three groups for wound coverage: (i) human ASCs-populated collagen gel, (ii) human dermal fibroblasts-populated collagen gel, and (iii) collagen gel alone. Wound contraction was prevented with a splint method. Wound size was measured 10 days after injury. At 28 days histological analysis was performed.
  
  
  RESULTS
  Both ASCs and dermal fibroblasts accelerated wound closure, but dermal fibroblasts were more effective than ASCs. At 28 days, the dermal portion of ASCs or dermal fibroblasts wound scars were thicker than collagen gel wound scars.
  
  
  CONCLUSIONS
  ASCs and dermal fibroblasts stimulate cutaneous wound healing and improve scar thickness.
authors: Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Korea.
authors: Seung Ho
authors: SH
authors: Lee
authors: Joon Ho
authors: JH
authors: Lee
authors: Kwang Hyun
authors: KH
authors: Cho