title: Construction and validation of two metagenomic DNA libraries from Cerrado soil with high clay content.
abstract: A challenge of metagenomic studies is in the extraction and purification of DNA from environmental samples. The soils of the Cerrado region of Brazil present several technical difficulties to DNA extraction: high clay content (>55% w/w), low pH (4.7) and high iron levels (146 ppm). Here we describe for the first time the efficient recovery and purification of microbial DNA associated with these unusual soil characteristics and the construction and validation of two metagenomic libraries: a 150,000 clones library with insert size of approximately 8 kb and a 65,000 clones library with insert size of approximately 35 kb. The construction of these metagenomic libraries will allow the biotechnological exploitation of the microbial community present in the soil from this endangered biome.
authors: Department de Biologia Celular, Instituto Central de Ciências Sul, Universidade de Brasília (UnB), Brasília, DF, Brazil. p.alinne@gmail.com
authors: Alinne Pereira
authors: AP
authors: de Castro
authors: Betania Ferraz
authors: BF
authors: Quirino
authors: Heather
authors: H
authors: Allen
authors: Lynn L
authors: LL
authors: Williamson
authors: Jo
authors: J
authors: Handelsman
authors: Ricardo Henrique
authors: RH
authors: Krüger
mesh_terms: D000538:Aluminum Silicates; D044822:Biodiversity; D001938:Brazil; D000077215:Clay; D015723:Gene Library; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis; D054892:Metagenome; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D000538:Aluminum Silicates; D012987:Soil; D007501:Iron; D000077215:Clay