title: Insomnia, platelet serotonin and platelet monoamine oxidase in chronic alcoholism.
abstract: Insomnia is a common sleep disorder frequently occurring in chronic alcoholic patients. Neurobiological basis of insomnia, as well as of alcoholism, is associated with disrupted functions of the main neurotransmitter systems, including the serotonin (5-hydroxytryptamine, 5-HT) system. Blood platelets are considered a limited peripheral model for the central 5-HT neurons, since both platelets and central 5-HT synaptosomes have similar dynamics of 5-HT. Platelet 5-HT concentration and platelet monoamine oxidase type B (MAO-B) are assumed to represent biomarkers for particular symptoms and behaviors in psychiatric disorders. The hypothesis of this study was that platelet 5-HT concentration and platelet MAO-B activity will be altered in chronic alcoholic patients with insomnia compared to comparable values in patients without insomnia. The study included 498 subjects: 395 male and 103 female medication-free patients with alcohol dependence and 502 healthy control subjects: 325 men and 177 women. The effects of early, middle and late insomnia (evaluated using the Hamilton Depression Rating Scale), as well as sex, age and smoking on platelet 5-HT concentration and platelet MAO-B activity were evaluated using one-way ANOVA and multiple regression analysis by the stepwise method. Platelet 5-HT concentration, but not platelet MAO-B activity, was significantly reduced in alcoholic patients with insomnia compared to patients without insomnia. Multiple regression analysis revealed that platelet 5-HT concentration was affected by middle insomnia, smoking and sex, while platelet MAO activity was affected only by sex and age. The present and previous data suggest that platelet 5-HT concentration might be used, after controlling for sex and smoking, as a biomarker for insomnia in alcoholism, PTSD and in rotating shift workers.
authors: Center for Alcoholism and Other Addictions, Psychiatric Hospital Vrapce, Bolnicka cesta 32, HR-1000 Zagreb, Croatia.
authors: Korona
authors: K
authors: Nenadic Sviglin
authors: Gordana
authors: G
authors: Nedic
authors: Matea
authors: M
authors: Nikolac
authors: Maja
authors: M
authors: Mustapic
authors: Dorotea
authors: D
authors: Muck-Seler
authors: Fran
authors: F
authors: Borovecki
authors: Nela
authors: N
authors: Pivac
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000437:Alcoholism / Q000097:blood* / Q000150:complications; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000097:blood*; D012701:Serotonin / Q000097:blood*; D012737:Sex Factors; D007319:Sleep Initiation and Maintenance Disorders / Q000097:blood* / Q000209:etiology; D012907:Smoking; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D012701:Serotonin; D008995:Monoamine Oxidase