title: Ultra high-performance liquid chromatography of porphyrins.
abstract: An ultra high-performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta-, hexa-, penta-carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow-rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias.
authors: Clinical Biochemistry, King's College Hospital, Denmark Hill, London, SE5 9RS, UK.
authors: Christopher M
authors: CM
authors: Benton
authors: Chang Kee
authors: CK
authors: Lim
authors: Harald J
authors: HJ
authors: Ritchie
authors: Caje
authors: C
authors: Moniz
authors: Donald J L
authors: DJ
authors: Jones
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces; D006801:Humans; D011164:Porphyrias / Q000378:metabolism / Q000652:urine; D011166:Porphyrins / Q000032:analysis*; D052616:Solid Phase Extraction; D013050:Spectrometry, Fluorescence
chemical_list: D011166:Porphyrins