title: Production of ε-poly-L-lysine by Streptomyces sp. using resin-based, in situ product removal.
abstract: Resin-based, in situ product removal (ISPR) was used to increase production of ε-poly-L-lysine (PL) by Streptomyces sp. GIM8. D152 resin was selected over Amberlite IRC-50, Amberlite IRC-76 and Amberlite IR-120 to develop ISPR using adsorption capacity and desorption ratio as bases. The yield of PL in response to external PL was unaffected in shake-flask culture; however, the production of PL increased to 2.9 from 0.8 g l(-1) shake-flasks using ISPR. In a 5 l fermentor, 23.4 g PL l(-1) was achieved compared to 3.76 g PL l(-1), in the controls by attaching two bags of D152 resin to the probes and baffles of the fermentor.
authors: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
authors: Shengrong
authors: S
authors: Liu
authors: Qingping
authors: Q
authors: Wu
authors: Jumei
authors: J
authors: Zhang
authors: Shuping
authors: S
authors: Mo
mesh_terms: D000327:Adsorption; D002411:Cation Exchange Resins / Q000737:chemistry / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D011107:Polylysine / Q000096:biosynthesis* / Q000302:isolation & purification; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D002411:Cation Exchange Resins; D011107:Polylysine