title: Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodium falciparum and P. vivax.
abstract: We compared light microscopy examination and a semi-nested multiplex PCR (SnM-PCR) assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small sub-unit ribosomal RNA (ssrRNA) gene sequences was applied. Microscopical examination showed that 48.5% (33.8% P. vivax and 14.7% P.falciparum) and 50% (35.3% P. vivax and 14.7% P. falciparum) of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy.
authors: Department of Medical Parasitology and Mycology, School of Public Health, Tehron University of Medical Sciences, Tehran, Islamic Republic of Iran.
authors: M
authors: M
authors: Noteghpour
authors: H
authors: H
authors: Abed Khojasteh
authors: H
authors: H
authors: Keshavarz
authors: H
authors: H
authors: Hajjaran
authors: Gh
authors: G
authors: Edrissian
authors: A
authors: A
authors: Rahimpi
authors: N
authors: N
authors: Gobakhloo
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000302:isolation & purification; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis*; D016780:Malaria, Vivax / Q000175:diagnosis*; D008297:Male; D008853:Microscopy / Q000295:instrumentation*; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan