title: Mycobacterium litorale sp. nov., a rapidly growing mycobacterium from soil.
abstract: A gram-positive, acid-fast and rapidly growing rod, designated F4(T), was isolated from a soil sample of Haikou in China. The isolate shared 98.2 % 16S rRNA gene sequence similarity with Mycobacterium monacense B9-21-178(T), 96.2 % hsp65 sequence similarity with M. monacense FI-05352 and 79.6 % 16S-23S rRNA internal transcribed spacer sequence similarity with M. monacense B9-21-178(T). DNA-DNA relatedness between the isolate and M. monacense DSM 44395(T) was 43.5 %. The morphological analysis and physiological tests also showed that the isolate differed from any strain reported to date. The mycolic acid profile and the cellular fatty acid composition were also determined. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, it was concluded that strain F4(T) ( = CGMCC 4.5724(T) = JCM 17423(T)) merited classification as the type strain of a novel species, for which the name Mycobacterium litorale sp. nov. is proposed.
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors: Yabo
authors: Y
authors: Zhang
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors: Jianli
authors: J
authors: Zhang
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors: Caiyuan
authors: C
authors: Fang
authors: Institute of Agri-resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors: Huancheng
authors: H
authors: Pang
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, PR China.
authors: Jinping
authors: J
authors: Fan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium