title: Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: implications for catecholamine secretion by PC12 cells.
abstract: PMCA1-4 isoforms have been recently recognised as regulators of various signalling pathways in mammalian cells. PMCAs were found to interact with calcineurin A in an isoform specific manner. In this study we focus on the interaction of calcineurin A with PMCA4 and its effect on catecholamine secretion in PC12 cells with reduced PMCA2 or PMCA3 content. Reduction of synthesis of PMCA2 or PMCA3 led to upregulation of PMCA4 manifested by preferential interaction of PMCA4 with calcineurin A. On the other hand, we observed a significant reduction of dopamine secretion, which did not correspond with an increased [Ca(2+)](c). This result indicates that the interaction of PMCA4 with calcineurin A plays a regulatory role in the signalling during catecholamine secretion.
authors: Department of Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland.
authors: Michalina
authors: M
authors: Kosiorek
authors: Paulina
authors: P
authors: Podszywalow-Bartnicka
authors: Ludmila
authors: L
authors: Zylinska
authors: Krzysztof
authors: K
authors: Zablocki
authors: Slawomir
authors: S
authors: Pikula
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D016716:PC12 Cells; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015854:Up-Regulation
chemical_list: D002395:Catecholamines; D007527:Isoenzymes; D019703:Calcineurin; D053499:Plasma Membrane Calcium-Transporting ATPases