title: Blood-based biomarkers can differentiate ulcerative colitis from Crohn's disease and noninflammatory diarrhea.
abstract: BACKGROUND
  Blood gene expression profiling has been used in several studies to identify patients with a number of conditions and diseases. A blood test with the ability to differentiate Crohn's disease (CD) from ulcerative colitis (UC) and noninflammatory diarrhea would be useful in the clinical management of these diseases.
  
  
  METHODS
  Affymetrix U133Plus 2.0 GeneChip oligonucleotide arrays were used to generate whole blood gene expression profiles for 21 patients with UC, 24 patients with CD, and 10 control patients with diarrhea, but without colonic pathology.
  
  
  RESULTS
  A supervised learning method (logistic regression) was used to identify specific panels of probe sets which were able to discriminate between UC and CD and from controls. The UC panel consisted of the four genes, CD300A, KPNA4, IL1R2, and ELAVL1; the CD panel comprised the four genes CAP1, BID, NIT2, and NPL. These panels clearly differentiated between CD and UC.
  
  
  CONCLUSIONS
  Gene expression profiles from blood can differentiate patients with CD from those with UC and from noninflammatory diarrheal disorders.
authors: Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA. rburakoff@partners.org
authors: Robert
authors: R
authors: Burakoff
authors: Samuel
authors: S
authors: Chao
authors: Molly
authors: M
authors: Perencevich
authors: Jay
authors: J
authors: Ying
authors: Sonia
authors: S
authors: Friedman
authors: Frederick
authors: F
authors: Makrauer
authors: Robert
authors: R
authors: Odze
authors: Hema
authors: H
authors: Khurana
authors: Choong-Chin
authors: CC
authors: Liew
mesh_terms: D000328:Adult; D000368:Aged; D000619:Aminohydrolases / Q000097:blood / Q000235:genetics; D015703:Antigens, CD / Q000097:blood / Q000235:genetics; D051023:BH3 Interacting Domain Death Agonist Protein / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000097:blood*; D018797:Cell Cycle Proteins / Q000097:blood / Q000235:genetics; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D003598:Cytoskeletal Proteins / Q000097:blood / Q000235:genetics; D003967:Diarrhea / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D051959:ELAV Proteins / Q000097:blood / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D007652:Oxo-Acid-Lyases / Q000097:blood / Q000235:genetics; D011971:Receptors, Immunologic / Q000097:blood / Q000235:genetics; D053574:Receptors, Interleukin-1 Type II / Q000097:blood / Q000235:genetics; D028901:alpha Karyopherins / Q000097:blood / Q000235:genetics
chemical_list: D015703:Antigens, CD; D051023:BH3 Interacting Domain Death Agonist Protein; C494814:BID protein, human; D015415:Biomarkers; C489549:CAP1 protein, human; C515070:CD300A protein, human; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D051959:ELAV Proteins; C508592:IL1R2 protein, human; C106259:KPNA4 protein, human; D011971:Receptors, Immunologic; D053574:Receptors, Interleukin-1 Type II; D028901:alpha Karyopherins; D000619:Aminohydrolases; C021867:nitrilase; D007652:Oxo-Acid-Lyases; C018728:N-acetylneuraminate lyase