title: Sulfation of estradiol in human epidermal keratinocyte.
abstract: Epidermis is one of the well-known estrogen target tissues. Information regarding estrogen metabolism in epidermis is still very limited compared to that of estrogen action. In the breast cancer tissue, 17β-estradiol (E(2)) is inactivated by sulfation and the expression level of estrogen sulfotransferase (SULT1E1) is inversely correlated with its malignancy. However, there is little datum about inactivation of estradiol in skin. In order to detect and measure E(2) and its metabolites simultaneously, we established an assay method with radio HPLC. A majority of [(3)H] labeled E(2) was converted to E(2) sulfate in normal human epidermal keratinocyte (NHEK) cells. The estimated activity of sulfotransferase toward E(2) at 20 nM was 0.11±0.01 (pmol/min/mg protein). Significant induction of estrogen sulfotransferase activity was observed in calcium-differentiated NHEK cells (0.58±0.07 (pmol/min/mg protein)). The gene expression of SULT1E1 was fifteen-fold higher in differentiated keratinocyte than in proliferating keratinocyte, whereas that of steroid sulfatase was reduced. These results suggest that E(2) inactivation is primarily mediated by SULT1E1 in keratinocyte and E(2) action is likely suppressed in epidermal differentiation.
authors: Faculty of Pharmacy, Keio University, 1–5–30 Shibakoen, Minato-ku, Tokyo 105–8512, Japan.
authors: Akira
authors: A
authors: Kushida
authors: Kenji
authors: K
authors: Hattori
authors: Nozomi
authors: N
authors: Yamaguchi
authors: Tetsuyuki
authors: T
authors: Kobayashi
authors: Akira
authors: A
authors: Date
authors: Hiroomi
authors: H
authors: Tamura
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013431:Sulfates / Q000378:metabolism*; D015238:Sulfotransferases / Q000378:metabolism
chemical_list: D017931:DNA Primers; D013431:Sulfates; D004958:Estradiol; D015238:Sulfotransferases; C020122:estrone sulfotransferase