title: Differential effects of kidney-lung cross-talk during acute kidney injury and bacterial pneumonia.
abstract: Acute injuries of the kidney or lung each represent serious, complex clinical problems, and their combination drastically decreases patient survival. However, detailed understanding of interactions between these two organs is scarce. To evaluate this further, we used the folic acid (FA) and myohemoglobinuria models of acute kidney injury (AKI) together with Pseudomonas aeruginosa inhalation to study kidney-lung cross-talk in mice during acute kidney and lung injury. Subgroups of mice received antineutrophil antibody or platelet-depleting serum to assess the role of neutrophil and platelets, respectively. AKI by itself did not cause clinically relevant acute lung injury. Pneumonia was neutrophil dependent, whereas pneumonia-induced AKI was platelet dependent. AKI attenuated pulmonary neutrophil recruitment and worsened pneumonia. Mice with AKI had lower oxygen saturations and greater bacterial load than mice without. Neutrophils isolated from mice with FA-induced AKI also had impaired transmigration and F-actin polymerization in vitro. Thus, during acute kidney and pneumonia-induced lung injury, clinically relevant kidney-lung interactions are both neutrophil and platelet dependent.
authors: Department of Critical Care Medicine, CRISMA Center, University of Pittsburgh, 3550 Terrace Street, Pittsburgh, PA 15261, USA. kas148@pitt.edu
authors: Kai
authors: K
authors: Singbartl
authors: Jeffery V
authors: JV
authors: Bishop
authors: Xiaoyan
authors: X
authors: Wen
authors: Raghavan
authors: R
authors: Murugan
authors: Saurabh
authors: S
authors: Chandra
authors: Marie-Dominique
authors: MD
authors: Filippi
authors: John A
authors: JA
authors: Kellum
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000150:complications* / Q000503:physiopathology*; D000818:Animals; D058491:Bacterial Load; D001792:Blood Platelets / Q000502:physiology; D004195:Disease Models, Animal; D005492:Folic Acid / Q000633:toxicity; D007668:Kidney / Q000503:physiopathology*; D008168:Lung / Q000382:microbiology / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology; D009928:Organ Specificity; D018410:Pneumonia, Bacterial / Q000150:complications* / Q000382:microbiology / Q000503:physiopathology*; D011552:Pseudomonas Infections / Q000150:complications / Q000382:microbiology / Q000503:physiopathology; D011550:Pseudomonas aeruginosa
chemical_list: D005492:Folic Acid