title: Development of a universal glycosyltransferase assay amenable to high-throughput formats.
abstract: The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
authors: Pharmaceutical Sciences Division, School of Pharmacy, Wisconsin Center for Natural Products Research, University of Wisconsin-Madison, Madison, WI 53705, USA.
authors: Hyun Soo
authors: HS
authors: Lee
authors: Jon S
authors: JS
authors: Thorson
mesh_terms: D001681:Biological Assay; D015374:Biosensing Techniques; D056831:Coordination Complexes / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry*; D016695:Glycosyltransferases / Q000737:chemistry* / Q000378:metabolism; D057166:High-Throughput Screening Assays; D007700:Kinetics; D015032:Zinc
chemical_list: D056831:Coordination Complexes; D015226:Dinucleoside Phosphates; D016695:Glycosyltransferases; D015032:Zinc