title: Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain.
abstract: Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.
authors: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing.
authors: Jiwei
authors: J
authors: Ding
authors: Lishan
authors: L
authors: Su
authors: Guangxia
authors: G
authors: Gao
mesh_terms: D015536:Down-Regulation; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005089:Exocytosis; D015870:Gene Expression; D020868:Gene Silencing / Q000187:drug effects*; D057809:HEK293 Cells; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D054730:Protein Interaction Domains and Motifs; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000494:pharmacology*; D014162:Transfection; D014771:Virion / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D057074:Virus Release / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate; C115635:citron-kinase; D017346:Protein Serine-Threonine Kinases