title: Live cell fluorescence microscopy techniques.
abstract: The use of fluorescent tags for in vivo tracking of proteins has provided an array of new data on cell function. Correspondingly, a variety of new methods utilizing these fluorescent tags have been developed. These methods must take into account all of the concerns of keeping live samples in conditions as close to physiological norms as possible, including temperature, CO(2) levels, media composition, and reduction of phototoxic effects. The microscope itself should also be designed to maximize the benefits and minimize the risks inherent in these methods. We provide an overview of these concerns.
authors: Bio-Imaging Resource Center, The Rockefeller University, New York, NY, USA.
authors: Shawn A
authors: SA
authors: Galdeen
authors: Alison J
authors: AJ
authors: North
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D003470:Culture Media; D005456:Fluorescent Dyes; D006801:Humans; D008027:Light; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D003470:Culture Media; D005456:Fluorescent Dyes