title: The number of the genes in a functional category matters during rat liver regeneration after partial hepatectomy.
abstract: Rat liver regeneration after partial hepatectomy (PH) is a good model to study the regulation of cell proliferation. We isolated hepatocytes from regenerating liver at different time points after PH and used microarray Rat Genome 230 2.0 chip to analyze the functional profiles of all up- or down-regulated genes manually and with automatic gene ontological tools. We found that the transcript expressions of PH and sham operation group were apparently different. For PH group, in the priming phase (2-12  h), signaling, transcription, response to stimulus genes predominated in up-regulated genes; in the proliferation phase (24-72 h), cell proliferation genes predominated; in the termination phase (120-168  h), differentiation and translation genes predominated; while metabolism genes predominated in the down-regulated genes at all time points (2-168  h). These functional profiles are consistent with the cellular and molecular phenomenon observed during liver regeneration, and can be closely connected with the biological process. Moreover, the results indicated that not only the quantity of specific genes but also the number of the genes in the specific functional category was regulated during liver regeneration, which means the number of similar genes in a specific functional category matters as well as the regulation of the genes. The changes of the number of the regulated cell proliferation genes and metabolism genes during liver regeneration were similar to the expression patterns of some cell division genes and metabolism genes.
authors: Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang, China.
authors: Yun
authors: Y
authors: Jiang
authors: Lian-Xing
authors: LX
authors: Zhang
authors: Cui-Fang
authors: CF
authors: Chang
authors: Gai-Ping
authors: GP
authors: Wang
authors: Rui-Jia
authors: RJ
authors: Shi
authors: Yan-Jie
authors: YJ
authors: Yang
authors: Cun-Shuan
authors: CS
authors: Xu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D006498:Hepatectomy; D008115:Liver Regeneration / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger