title: Estrogen promotes B cell activation in vitro through down-regulating CD80 molecule expression.
abstract: Estrogen is the main female hormone of women. It has diverse effects on cell growth, differentiation and homeostatic functions. Accumulated evidence has indicated that estrogen may regulate multiple immune functions and the immune status of women. However, there is little report on the effect of estrogen on mature B cell functions. In this study, we observed the effect of 17β-estradiol (E2) on the proliferation, apoptosis, antibody production and differentiation of splenic B cells of mice in vitro. Splenocytes of female BALB/c mice were isolated and cultured with E2. E2 treatments decreased the expression of CD80 molecule on splenic B cells but enhanced the total IgG antibody production of splenocyte, without promoting the differentiation of B cells to plasma cells. E2 protected splenic B cells from the serum-deficiency-induced apoptosis but had no influence on the proliferation of B cells. These results suggest that estrogen may promote the activity of B cells through down-regulating the expression of CD80 molecule on B cells.
authors: Key Laboratory for Reproductive Medicine of Shandong Province, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, PR China.
authors: Yibing
authors: Y
authors: Fu
authors: Li
authors: L
authors: Li
authors: Xiaowen
authors: X
authors: Liu
authors: Chunyan
authors: C
authors: Ma
authors: Jie
authors: J
authors: Zhang
authors: Yulian
authors: Y
authors: Jiao
authors: Li
authors: L
authors: You
authors: Zi-Jiang
authors: ZJ
authors: Chen
authors: Yueran
authors: Y
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism*; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000378:metabolism; D015536:Down-Regulation; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009994:Osmolar Concentration; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D018122:B7-1 Antigen; D016895:Culture Media, Serum-Free; D004967:Estrogens; D007074:Immunoglobulin G; D004958:Estradiol