title: The protective effect of magnesium lithospermate B against glucose-induced intracellular oxidative damage.
abstract: OBJECTIVE
  To investigate the effects of magnesium lithospermate B (LAB) on intracellular reactive oxygen species (ROS) production induced by high dose of glucose or H(2)O(2), we explored the influences of LAB on the expression of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf2) in HEK293T cells after treatment with high dose of glucose.
  
  
  METHODS
  The total nuclear proteins in HEK293T cells were extracted with Cytoplasmic Protein Extraction Kit. The ROS level was determined by flow cytometry. The mRNA and protein expression of HO-1 and Nrf2 were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot.
  
  
  RESULTS
  LAB reduced the ROS production in HEK293T cells cultured under oxidative stress. High dose of glucose enhanced the expression of HO-1 mRNA and HO-1 protein in a time-dependent manner. LAB enhanced the expression of HO-1 mRNA and HO-1 protein in a dose-dependent manner treated with high dose of glucose. The amount of Nrf2 translocation was enhanced after cells were pretreated with 50μmol/L or 100μmol/L LAB. Silencing of Nrf2 gene eliminated the enhanced expression of HO-1 protein induced by high dose of glucose plus LAB.
  
  
  CONCLUSIONS
  LAB plays an important role against glucose-induced intracellular oxidative damage. The enhanced expression of HO-1 mRNA and HO-1 protein caused by LAB is regulated via Nrf2 signal pathway.
authors: Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Xiangya School of Medicine, Changsha 410078, China.
authors: Jian
authors: J
authors: Qu
authors: Xian
authors: X
authors: Ren
authors: Rui-ying
authors: RY
authors: Hou
authors: Xing-ping
authors: XP
authors: Dai
authors: Ying-chun
authors: YC
authors: Zhao
authors: Xiao-jing
authors: XJ
authors: Xu
authors: Wei
authors: W
authors: Zhang
authors: Gan
authors: G
authors: Zhou
authors: Hong-hao
authors: HH
authors: Zhou
authors: Zhao-qian
authors: ZQ
authors: Liu
mesh_terms: D001483:Base Sequence; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005947:Glucose / Q000633:toxicity*; D057809:HEK293 Cells; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D008969:Molecular Sequence Data; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D051267:NF-E2-Related Factor 2; D017382:Reactive Oxygen Species; C061091:lithospermate B; D006861:Hydrogen Peroxide; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D005947:Glucose