title: Gene expression in accumbens GABA neurons from inbred rats with different drug-taking behavior.
abstract: Inbred Lewis and Fisher 344 rat strains differ greatly in drug self-administration; Lewis rats operantly self-administer drugs of abuse including nicotine, whereas Fisher self-administer poorly. As shown herein, operant food self-administration is similar. On the basis of their pivotal role in drug reward, we hypothesized that differences in basal gene expression in GABAergic neurons projecting from nucleus accumbens (NAcc) to ventral pallidum (VP) play a role in vulnerability to drug-taking behavior. The transcriptomes of NAcc shell-VP GABAergic neurons from these two strains were analyzed in adolescents, using a multidisciplinary approach that combined stereotaxic ionotophoretic brain microinjections, laser-capture microdissection (LCM) and microarray measurement of transcripts. Laser-capture microdissection enriched the gene transcripts detected in gamma-aminobutyric acid (GABA) neurons compared to the residual NAcc tissue: a ratio of neuron/residual >1 and false discovery rate (FDR) <5% yielded 6623 transcripts, whereas a ratio of >3 yielded 3514. Strain-dependent differences in gene expression within GABA neurons were identified; 322 vs. 60 transcripts showed 1.5-fold vs. 2-fold differences in expression (FDR < 5%). Classification by gene ontology showed that these 322 transcripts were widely distributed, without categorical enrichment. This is most consistent with a global change in GABA neuron function. Literature mining by Chilibot found 38 genes related to synaptic plasticity, signaling and gene transcription, all of which determine drug abuse; 33 genes have no known association with addiction or nicotine. In Lewis rats, upregulation of Mint-1, Cask, CamkII , Ncam1, Vsnl1, Hpcal1 and Car8 indicates that these transcripts likely contribute to altered signaling and synaptic function in NAcc GABA projection neurons to VP.
authors: Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA. bsharp@uthsc.edu
authors: B M
authors: BM
authors: Sharp
authors: H
authors: H
authors: Chen
authors: S
authors: S
authors: Gong
authors: X
authors: X
authors: Wu
authors: Z
authors: Z
authors: Liu
authors: K
authors: K
authors: Hiler
authors: W L
authors: WL
authors: Taylor
authors: S G
authors: SG
authors: Matta
mesh_terms: D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D003216:Conditioning, Operant / Q000502:physiology; D004195:Disease Models, Animal; D059330:GABAergic Neurons / Q000378:metabolism*; D020869:Gene Expression Profiling; D005917:Globus Pallidus / Q000166:cytology / Q000378:metabolism; D061346:Laser Capture Microdissection; D008297:Male; D009042:Motivation / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000145:classification / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D009714:Nucleus Accumbens / Q000166:cytology / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D012054:Reinforcement, Psychology; D013045:Species Specificity; D019966:Substance-Related Disorders / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D012313:RNA