title: SNCG shRNA suppressed breast cancer cell xenograft formation and growth in nude mice.
abstract: BACKGROUND
  Overexpression of breast cancer-specific gene 1 (SNCG) is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA (shRNA).
  
  
  METHODS
  Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors. These vectors were then stably transfected into a breast cancer MCF-7 cell line to knockdown SNCG expression. After SNCG knockdown was confirmed, the stable cell lines were inoculated into nude mice. SNCG mRNA and protein expressions were analyzed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry, respectively in both the stable cell lines and xenografts.
  
  
  RESULTS
  All four SNCG shRNA constructs significantly reduced SNCG mRNA and protein levels in MCF-7 cells, as compared to the unrelated sequence control shRNA and the liposome control mice (P < 0.05). SNCG-knockdown MCF-7 cells formed significantly smaller tumor masses than cells expressing the unrelated sequence control or the liposome control mice (P < 0.05).
  
  
  CONCLUSIONS
  SNCG shRNA effectively suppressed breast cancer cell formation in vivo and may be a useful clinical strategy to control breast cancer.
authors: Department of Oncology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450051, China.
authors: Pei-Hong
authors: PH
authors: Shen
authors: Qing-Xia
authors: QX
authors: Fan
authors: Yan-Wei
authors: YW
authors: Li
authors: Wei
authors: W
authors: Zhang
authors: Xiao-Kai
authors: XK
authors: He
authors: Zhen
authors: Z
authors: Wang
authors: Yun-Han
authors: YH
authors: Zhang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023041:Xenograft Model Antitumor Assays; D051845:gamma-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D051845:gamma-Synuclein