title: Investigation of DNA binding mechanism, photoinduced cleavage activity, electrochemical properties and biological functions of mixed ligand copper(II) complexes with benzimidazole derivatives: synthesis and spectral characterization.
abstract: The benzimidazole derivative Schiff bases and their copper(II) (Cu(II)) mixed-polypyridyl complexes (1-4) have been synthesized and characterized by the spectral and analytical techniques. DNA binding/cleavage studies indicate a stronger binding capability for the complex 4 which is confirmed by the absorbance, viscometric and gel-electrophoresis studies. The photocleavage of plasmid pBR322 DNA reveals that hydroxyl radical (OH(•)) and singlet oxygen ((1)O(2)) are likely to be the reactive species. Analysis of the growth activity shows that the antimicrobial effect of these Schiff bases on Gram-negative bacteria is higher than that on Gram-positive. Furthermore, the complexes having nitro group show an increased antimicrobial effect.
authors: Research Department of Chemistry, VHNSN College, Virudhunagar, India. drn_raman@yahoo.co.in
authors: Natarajan
authors: N
authors: Raman
authors: Abraham
authors: A
authors: Selvan
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001562:Benzimidazoles / Q000737:chemistry*; D001665:Binding Sites / Q000187:drug effects; D003300:Copper / Q000737:chemistry*; D053837:DNA Cleavage; D004269:DNA, Bacterial / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D055664:Electrochemical Techniques; D008024:Ligands; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D055668:Photochemical Processes; D010957:Plasmids; D012545:Schiff Bases / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D001562:Benzimidazoles; D004269:DNA, Bacterial; D008024:Ligands; D009942:Organometallic Compounds; D012545:Schiff Bases; D003300:Copper; C031000:benzimidazole