title: Susceptibility of Vaccinia virus to chemical disinfectants.
abstract: Vaccinia virus (VACV) is the cause of bovine vaccinia (BV), an emerging zoonotic disease that affects dairy cows and milkers. Some chemical disinfectants have been used on farms affected by BV to disinfect cow teats and milkers' hands. To date, there is no information about the efficacy of disinfectants against VACV. Therefore, this study aimed to assess the virucidal activity of some active disinfectants commonly used in the field. Sodium hypochlorite, quaternary ammonium combined with chlorhexidine, and quaternary ammonium combined with glutaraldehyde were effective in inactivating the virus at all concentrations tested. Iodine and quaternary ammonium as the only active component were partially effective. The presence of bovine feces as organic matter and light decreased the effectiveness of sodium hypochlorite. These results show that an appropriated disinfection and asepsis of teats and hands may be helpful in the control and prevention of BV and other infections with VACV.
authors: Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, e Laboratório de Vírus, Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. tercialud@gmail.com
authors: Tércia Moreira Ludolfo
authors: TM
authors: de Oliveira
authors: Izabelle Silva
authors: IS
authors: Rehfeld
authors: Maria Isabel Maldonado
authors: MI
authors: Coelho Guedes
authors: Jaqueline Maria Siqueira
authors: JM
authors: Ferreira
authors: Erna Geessien
authors: EG
authors: Kroon
authors: Zélia Inês Portela
authors: ZI
authors: Lobato
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004202:Disinfectants / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D014616:Vaccinia virus / Q000187:drug effects*; D014709:Vero Cells
chemical_list: D004202:Disinfectants