title: Biomimetic bone-like composites fabricated through an automated alternate soaking process.
abstract: Hydroxyapatite-gelatin composites have been proposed as suitable scaffolds for bone and dentin tissue regeneration. There is considerable interest in producing these scaffolds using biomimetic methods due to their low energy costs and potential to create composites similar to the tissues they are intended to replace. Here an existing process used to coat a surface with hydroxyapatite under near physiological conditions, the alternate soaking process, is modified and automated using an inexpensive "off the shelf" robotics kit. The process is initially used to precipitate calcium phosphate coatings. Then, in contrast to previous utilizations of the alternate soaking process, gelatin was added directly to the solutions in order to co-precipitate hydroxyapatite-gelatin composites. Samples were investigated by Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and nanoindentation. Calcium phosphate coatings formed by the alternate soaking process exhibited different calcium to phosphate ratios, with correspondingly distinct structural morphologies. The coatings demonstrated an interconnected structure with measurable mechanical properties, even though they were 95% porous. In contrast, hydroxyapatite-gelatin composite coatings over 2mm thick could be formed with little visible porosity. The hydroxyapatite-gelatin composites demonstrate a composition and mechanical properties similar to those of cortical bone.
authors: Engineering Department, Cambridge University, Cambridge CB2 1PZ, UK.
authors: Daniel G T
authors: DG
authors: Strange
authors: Michelle L
authors: ML
authors: Oyen
mesh_terms: D001331:Automation; D040761:Biomimetic Materials / Q000494:pharmacology*; D032701:Biomimetics / Q000379:methods*; D001842:Bone and Bones / Q000187:drug effects*; D017886:Durapatite / Q000737:chemistry; D005780:Gelatin / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D012996:Solutions; D005780:Gelatin; D017886:Durapatite