title: Monolith-based immobilized metal affinity chromatography increases production efficiency for plasmid DNA purification.
abstract: Immobilized metal affinity monolith column as a new class of chromatographic support is shown to be superior to conventional particle-based column as plasmid DNA (pDNA) purification platform. By harnessing the affinity of endotoxin to copper ions in the solution, a majority of endotoxin (90%) was removed from the alkaline cell lysate using CuCl(2)-induced precipitation. RNA and remaining endotoxin were subsequently removed to below detection limit with minimal loss of pDNA using either monolith or particle-based column. Monolith column has the additional advantage of feed concentration and flowrate-independent dynamic binding capacity for RNA molecules, enabling purification process to be conducted at high feed RNA concentration and flowrate. The use of monolith column gives three fold increased productivity of pDNA as compared to particle-based column, providing a more rapid and economical platform for pDNA purification.
authors: School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, South Korea.
authors: Min Jae
authors: MJ
authors: Shin
authors: Lihan
authors: L
authors: Tan
authors: Min Ho
authors: MH
authors: Jeong
authors: Ji-Heung
authors: JH
authors: Kim
authors: Woo-Seok
authors: WS
authors: Choe
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D008670:Metals / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D008670:Metals; D012117:Resins, Synthetic; D004247:DNA