title: Neuron-specific impairment of inter-chromosomal pairing and transcription in a novel model of human 15q-duplication syndrome.
abstract: Although the etiology of autism remains largely unknown, cytogenetic and genetic studies have implicated maternal copy number gains of 15q11-q13 in 1-3% of autism cases. In order to understand how maternal 15q duplication leads to dysregulation of gene expression and altered chromatin interactions, we used microcell-mediated chromosome transfer to generate a novel maternal 15q duplication model in a human neuronal cell line. Our 15q duplication neuronal model revealed that by quantitative RT-PCR, transcript levels of NDN, SNRPN, GABRB3 and CHRNA7 were reduced compared with expected levels despite having no detectable alteration in promoter DNA methylation. Since 15q11-q13 alleles have been previously shown to exhibit homologous pairing in mature human neurons, we assessed homologous pairing of 15q11-q13 by fluorescence in situ hybridization. Homologous pairing of 15q11-q13 was significantly disrupted by 15q duplication. To further understand the extent and mechanism of 15q11-q13 homologous pairing, we mapped the minimal region of homologous pairing to a ∼500 kb region at the 3' end of GABRB3 which contains multiple binding sites for chromatin regulators MeCP2 and CTCF. Both active transcription and the chromatin factors MeCP2 and CTCF are required for the homologous pairing of 15q11-q13 during neuronal maturational differentiation. These data support a model where 15q11-q13 genes are regulated epigenetically at the level of both inter- and intra-chromosomal associations and that chromosome imbalance disrupts the epigenetic regulation of genes in 15q11-q13.
authors: Frontier Science Organization, Kanazawa University, 13-1 Takaramachi, Kanazawa, Japan. sihorike@staff.kanazawa-u.ac.jp
authors: Makiko
authors: M
authors: Meguro-Horike
authors: Dag H
authors: DH
authors: Yasui
authors: Weston
authors: W
authors: Powell
authors: Diane I
authors: DI
authors: Schroeder
authors: Mitsuo
authors: M
authors: Oshimura
authors: Janine M
authors: JM
authors: Lasalle
authors: Shin-ichi
authors: S
authors: Horike
mesh_terms: D000818:Animals; D000076246:CCCTC-Binding Factor; D002454:Cell Differentiation; D002460:Cell Line; D002659:Child Development Disorders, Pervasive / Q000235:genetics* / Q000378:metabolism; D002675:Child, Preschool; D058674:Chromosome Duplication; D023902:Chromosome Pairing; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D020440:Gene Duplication; D018392:Genomic Imprinting; D006801:Humans; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014314:Trisomy / Q000235:genetics*
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; C000619698:Ctcf protein, mouse; D051783:Methyl-CpG-Binding Protein 2; D012097:Repressor Proteins