title: Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.).
abstract: Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a LEA gene, CarLEA4 (GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG). CarLEA4 contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of CarLEA4 were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds. CarLEA4 was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of CarLEA4 was strongly induced by drought, salt, heat, cold, ABA, IAA, GA(3) and MeJA. Our results suggest that CarLEA4 encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.
authors: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
authors: Hanyan
authors: H
authors: Gu
authors: Yuying
authors: Y
authors: Jia
authors: Xiansheng
authors: X
authors: Wang
authors: Quanjia
authors: Q
authors: Chen
authors: Shubing
authors: S
authors: Shi
authors: Lin
authors: L
authors: Ma
authors: Jusong
authors: J
authors: Zhang
authors: Hua
authors: H
authors: Zhang
authors: Hao
authors: H
authors: Ma
mesh_terms: D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000378:metabolism; D029863:Cicer / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009928:Organ Specificity / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; C066246:late embryogenesis abundant protein, plant; D049452:Green Fluorescent Proteins