title: Biodegradable and biocompatible biomaterial, polyhydroxybutyrate, produced by an indigenous Vibrio sp. BM-1 isolated from marine environment.
abstract: Polyhydroxybutyrate (PHB) is one of the polyhydroxyalkanoates (PHAs) which has biodegradable and biocompatible properties. They are adopted in the biomedical field, in, for example, medical implants and drug delivery carriers. This study seeks to promote the production of PHB by Vibrio sp. BM-1, isolated from a marine environment by improving constituents of medium and implementing an appropriate fermentation strategy. This study successfully developed a glycerol-yeast extract-tryptone (GYT) medium that can facilitate the growth of Vibrio sp. BM-1 and lead to the production of 1.4 g/L PHB at 20 h cultivation. This study also shows that 1.57 g/L PHB concentration and 16% PHB content were achieved, respectively, when Vibrio sp. BM-1 was cultivated with MS-GYT medium (mineral salts-supplemented GYT medium) for 12 h. Both cell dry weight (CDW) and residual CDW remained constant at around 8.2 g/L and 8.0 g/L after the 12 h of cultivation, until the end of the experiment. However, both 16% of PHB content and 1.57 g/L of PHB production decreased rapidly to 3% and 0.25 g/L, respectively from 12 h of cultivation to 40 h of cultivation. The results suggest that the secretion of PHB depolymerase that might be caused by the addition of mineral salts reduced PHB after 12 h of cultivation. However, work will be done to explain the effect of adding mineral salts on the production of PHB by Vibrio sp. BM-1 in the near future.
authors: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors: Yu-Hong
authors: YH
authors: Wei
authors: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors: Wei-Chuan
authors: WC
authors: Chen
authors: Department of Chemical Engineering and Material Science, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors: Ho-Shing
authors: HS
authors: Wu
authors: Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan 320, Taiwan.
authors: Om-Murugan
authors: OM
authors: Janarthanan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D008903:Minerals / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D012492:Salts / Q000737:chemistry; D013997:Time Factors; D014733:Vibrio / Q000254:growth & development / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D002265:Carboxylic Ester Hydrolases; D003470:Culture Media; D006885:Hydroxybutyrates; D008903:Minerals; D011091:Polyesters; D012492:Salts; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C022417:poly-beta-hydroxybutyrate depolymerase