title: EMMPRIN and its ligand cyclophilin A as novel diagnostic markers in inflammatory cardiomyopathy.
abstract: BACKGROUND
  During inflammatory cardiomyopathy matrix metalloproteinases are crucially involved in cardiac remodeling. The aim of the present study was to investigate whether the "extracellular matrix metalloproteinase inducer" EMMPRIN (CD147) and its ligand Cyclophilin A (CyPA) are upregulated in inflammatory cardiomyopathy and may serve as diagnostic markers. Therefore, a series of 102 human endomyocardial biopsies were analyzed for the expression of EMMPRIN and CyPA and correlated with histological and immunohistological findings.
  
  
  RESULTS
  Endomyocardial biopsies were stained for EMMPRIN and CyPA in addition to standard histology (HE, Trichrom) and immunohistological stainings (MHC-II, CD68, CD3). 39 (38.2%) biopsies met the immunohistological criteria of an inflammatory cardiomyopathy. EMMPRIN, which was predominantly expressed on cardiomyocytes, was slightly (but significantly) upregulated in non inflammatory cardiomyopathies compared to normal histopathological findings and highly upregulated in inflammatory cardiomyopathy compared to both non inflammatory cardiomyopathy and normal histopathology. In contrast, CyPA reveals no enhanced expression in non inflammatory cardiomyopathies and a highly enhanced expression in inflammatory cardiomyopathy, where it is closely associated with leucocytes infiltrates. We found a strong correlation between both EMMPRIN and CyPA with the expression of MHC-II molecules (correlation coefficient 0.475 and 0.527, p<0.05). Moreover, we found a correlation for both EMMPRIN and CyPA with CD68 (correlation coefficient 0.393 and 0.387, p<0.05) and CD3 (correlation coefficient 0.360 and 0.235, p<0.05).
  
  
  CONCLUSIONS
  EMMPRIN is enhanced in both inflammatory and non inflammatory cardiomyopathies and can serve as a marker of myocardial remodeling. CyPA may represent a novel and specific marker for cardiac inflammation.
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Peter
authors: P
authors: Seizer
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Tobias
authors: T
authors: Geisler
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Boris
authors: B
authors: Bigalke
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Martin
authors: M
authors: Schneider
authors: Abteilung für Molekulare Pathologie, Eberhard Karls Universität Tübingen, Germany.
authors: Karin
authors: K
authors: Klingel
authors: Abteilung für Molekulare Pathologie, Eberhard Karls Universität Tübingen, Germany.
authors: Reinhard
authors: R
authors: Kandolf
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Konstantinos
authors: K
authors: Stellos
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Jürgen
authors: J
authors: Schreieck
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany.
authors: Meinrad
authors: M
authors: Gawaz
authors: Medizinische Klinik III, Kardiologie und Kreislauferkrankungen, Germany. Electronic address: andreas.may@med.uni-tuebingen.de.
authors: Andreas E
authors: AE
authors: May
mesh_terms: D000328:Adult; D000368:Aged; D051926:Basigin / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D009202:Cardiomyopathies / Q000175:diagnosis* / Q000378:metabolism*; D021984:Cyclophilin A / Q000378:metabolism*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008024:Ligands; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*
chemical_list: C497886:BSG protein, human; D015415:Biomarkers; D018836:Inflammation Mediators; D008024:Ligands; D051926:Basigin; D021984:Cyclophilin A