title: Capillary zone electrophoresis for the analysis of glycoforms of cellobiohydrolase.
abstract: Cellobiohydrolase (CBH) is an important enzyme for the conversion of lignocellulosic biomass to ethanol. This work separated the glycoforms of CBH possessing different numbers of neutral mannoses using capillary zone electrophoresis (CZE) in a 50 mM, pH 7.5 phosphate buffer. The method analysed CBH in an intact form using a polyacrylamide coated fused silica capillary without requiring additives or labelling of the enzyme. The migration time of the major peak was found to be 21.6±0.1 min (n=3) and the approach is suitable for testing of batch-to-batch consistency of CBH. Ease-of-use, automation and speed are the other benefits due to which the use of CZE for analysing glycoforms of CBH was concluded to be ideal.
authors: School of Electrical and Electronics Engineering, The University of Manchester, Sackville Street Building, Sackville Street, Manchester M13 9PL, UK. ruchi.gupta@postgrad.manchester.ac.uk
authors: Ruchi
authors: R
authors: Gupta
authors: Sara J
authors: SJ
authors: Baldock
authors: Peter R
authors: PR
authors: Fielden
authors: Bruce D
authors: BD
authors: Grieve
mesh_terms: D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000379:methods; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D014242:Trichoderma / Q000201:enzymology
chemical_list: D005656:Fungal Proteins; D043366:Cellulose 1,4-beta-Cellobiosidase