title: Detection of Porphyromonas gulae from subgingival biofilms of dogs with and without periodontitis.
abstract: A rapid PCR approach was developed to detect Porphyromonas gulae strains from subgingival samples of dogs with and with periodontitis. The presence of P. gulae was observed in 92% and 56%, respectively, in dogs with and without periodontitis. The new primer pair was specific to detect this microorganism, and this technique could be used to evaluate a correlation between periodontitis and P. gulae in companion animals.
authors: Anaerobe Laboratory, Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Brazil.
authors: Gerusa Neyla Andrade
authors: GN
authors: Senhorinho
authors: Viviane
authors: V
authors: Nakano
authors: Chengxu
authors: C
authors: Liu
authors: Yuli
authors: Y
authors: Song
authors: Sydney M
authors: SM
authors: Finegold
authors: Mario J
authors: MJ
authors: Avila-Campos
mesh_terms: D000818:Animals; D016866:Bacteroidaceae Infections / Q000382:microbiology / Q000662:veterinary*; D018441:Biofilms; D017931:DNA Primers; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005881:Gingiva / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017861:Porphyromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S