title: Oxygen and cytokine-dependent changes in choline phospholipid saturation in hematopoietic progenitor cells detected by MALDI-TOF mass spectrometry.
abstract: The adaptation of cells to a changing environment is normally accompanied by rapid and/or chronic remodeling of membrane lipids. In order to understand the role played by membrane lipid metabolism in such responses, it is necessary to characterize in more detail the changes in membrane composition occurring in response to defined stimuli. There has been intense interest in characterizing the "stem cell niche" in recent years and an emerging consensus that stem cells are located in regions of low oxygen tension and probably well-isolated from the blood supply. We report here the use of matrix-assisted laser desorption and ionization time-of-flight mass spectrometry to monitor changes in the composition and saturation degree of choline phospholipids of hematopoietic progenitor (FDCPmix) cells under standard nutrient-rich culture conditions and at low oxygen and low glucose concentrations. We found that the increase in proliferation rate driven by high concentrations of interleukin-3 (IL-3) is associated with a decrease in membrane phosphatidylcholine (PC) 18:0/20:4 and sphingomyelin (SM) together with an increase in PC 18:0/18:2 and dihydro SM. Furthermore, this effect is most pronounced under low oxygen and low glucose conditions, independent of cell proliferation rates.
authors: Institute of Medical Physics and Biophysics, University of Leipzig, Medical Faculty, Härtelstr. 16-18, D-04107 Leipzig, Germany. Beate.Fuchs@medizin.uni-leipzig.de
authors: Beate
authors: B
authors: Fuchs
authors: Lydia
authors: L
authors: Schnapka-Hille
authors: Jürgen
authors: J
authors: Schiller
authors: Michael A
authors: MA
authors: Cross
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005947:Glucose / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D010100:Oxygen / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013109:Sphingomyelins / Q000032:analysis
chemical_list: D007377:Interleukin-3; D010713:Phosphatidylcholines; D013109:Sphingomyelins; D005947:Glucose; D010100:Oxygen