title: Experimental and numerical analysis of high-resolution injection technique for capillary electrophoresis microchip.
abstract: This study presents an experimental and numerical investigation on the use of high-resolution injection techniques to deliver sample plugs within a capillary electrophoresis (CE) microchip. The CE microfluidic device was integrated into a U-shaped injection system and an expansion chamber located at the inlet of the separation channel, which can miniize the sample leakage effect and deliver a high-quality sample plug into the separation channel so that the detection performance of the device is enhanced. The proposed 45° U-shaped injection system was investigated using a sample of Rhodamine B dye. Meanwhile, the analysis of the current CE microfluidic chip was studied by considering the separation of Hae III digested ϕx-174 DNA samples. The experimental and numerical results indicate that the included 45° U-shaped injector completely eliminates the sample leakage and an expansion separation channel with an expansion ratio of 2.5 delivers a sample plug with a perfect detection shape and highest concentration intensity, hence enabling an optimal injection and separation performance.
authors: Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan; E-Mails: clchang@mail.npust.edu.tw (C.-L.C.); jcleong@mail.npust.edu.tw (J.-C.L.).
authors: Chin-Lung
authors: CL
authors: Chang
authors: Jik-Chang
authors: JC
authors: Leong
authors: Ting-Fu
authors: TF
authors: Hong
authors: Yao-Nan
authors: YN
authors: Wang
authors: Lung-Ming
authors: LM
authors: Fu
mesh_terms: D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D046209:Electrophoresis, Microchip / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases