title: Microbore liquid chromatography with UV detection to study the in vivo passage of compound 21, a non-peptidergic AT₂ receptor agonist, to the striatum in rats.
abstract: A microbore liquid chromatography method coupled to UV detection was developed and validated in order to monitor the passage of compound 21 (C21), a non-peptide angiotensin II type 2 receptor agonist, to the striatum of rats. For this purpose, sampling from the striatum was performed using the in vivo microdialysis technique. Separations were performed on a C₁₈ microbore (1mm i.d.) column using gradient elution. The retention time for C21 was found to be 6.3 min. The calibration curve was linear between 10 and 200 ng/ml with a correlation coefficient ≥ 0.999. The limit of detection and the limit of quantification were 3 and 10 ng/ml respectively. The intra-day and the inter-day precision (RSD%) ranged between 0.5 and 4.6% with an average recovery of 101.5±10.0% (mean±SD, n=15). In vivo experiments were performed on rats to measure the concentration of C21 in striatal dialysates after intraperitoneal (10 or 50mg/kg) or intravenous injection (10 mg/kg or 20 mg/kg) of C21 and suggest minimal passage of the compound to the striatum.
authors: Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Laarbeeklaan 103, B-1090 Brussels, Belgium.
authors: Naser
authors: N
authors: Shraim
authors: Birgit
authors: B
authors: Mertens
authors: Ralph
authors: R
authors: Clinckers
authors: Sophie
authors: S
authors: Sarre
authors: Yvette
authors: Y
authors: Michotte
authors: Ann
authors: A
authors: Van Eeckhaut
mesh_terms: D000818:Animals; D002219:Carbamates / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D002853:Chromatography, Liquid / Q000379:methods*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000379:methods*; D009480:Neuropharmacology / Q000379:methods*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D044139:Receptor, Angiotensin, Type 2 / Q000819:agonists*; D013449:Sulfonamides / Q000737:chemistry / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D002219:Carbamates; D018696:Neuroprotective Agents; D044139:Receptor, Angiotensin, Type 2; D013449:Sulfonamides