title: Chitinase production in solid-state fermentation from Oerskovia xanthineolytica NCIM 2839 and its application in fungal protoplasts formation.
abstract: The present study reports the economic production of thermostable chitinase production from Oerskovia xanthineolytica NCIM 2839 by solid-state fermentation (SSF) technique and its application in fungal protoplasts formation. The Oerskovia xanthineolytica NCIM 2839 was found to produce thermostable chitinase 148 U g(-1) of solid substrate in SSF using wheat bran with colloidal chitin as base. Protoplasts of A. niger were formed by using crude chitinase produced in SSF and formed protoplasts were confirmed by using scanning electron microscopy. This is the simple and economical method for protoplast formation which makes it possible applications in strain improvement of various fungi by protoplasts fusion in Biotechnological industries.
authors: Department of Microbiology, Shivaji University, Vidyanagar, Kolhapur 416004, India. shlshwaghmare@gmail.com
authors: Shailesh R
authors: SR
authors: Waghmare
authors: Swaroop S
authors: SS
authors: Kulkarni
authors: Jai S
authors: JS
authors: Ghosh
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000254:growth & development*; D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D001709:Biotechnology / Q000379:methods; D002473:Cell Wall / Q000378:metabolism; D002688:Chitinases / Q000302:isolation & purification* / Q000378:metabolism*; D005285:Fermentation; D008855:Microscopy, Electron, Scanning; D011523:Protoplasts
chemical_list: D002688:Chitinases