title: Changes of glycogen metabolism in the gills and hepatic tissue of tilapia (Oreochromis mossambicus) during short-term Cd exposure.
abstract: The aim of the study was to test the hypothesis that the mechanism of glycogen metabolism has taken place in gills rather than in liver during Cd exposure. Male tilapia were exposed to 44.45 μM ambient Cd for 12h, and we found blood glucose significantly increased, however, lactate levels showed no significant changes. The glycogen phosphorylase (GP) activity increased immediately after 0.75 to 3h of Cd exposure in the gills, and after 1 to 6h in the liver, respectively. In addition, the glycogen level depleted faster in the gills than in the liver. Plasma cortisol level increased from 0.25 to 1h and recovered after 3h, while the glucagon did not significantly change during Cd exposure. Glucocorticoid receptor (GR) mRNA expression decreased after 0.75 h in the gills, while it significantly increased after 6h in the liver. Ca(2+), Na(+), Cl(-), and K(+) significantly decreased upon Cd exposure within 6h following Cd-induced toxic stress. We suggested that the cortisol is the spontaneous stimulation of glycogen metabolism in the gills, and it triggers a subsequent energy supply later in the liver. Taken together, the profile of glycogen metabolism between gills and liver during Cd-exposure stress provide good support to our hypothesis.
authors: Department of Aquatic Biosciences, National Chiayi University, Chiayi 600, Taiwan.
authors: Yu-Siang
authors: YS
authors: Lin
authors: Shu-Chuan
authors: SC
authors: Tsai
authors: Hui-Chen
authors: HC
authors: Lin
authors: Chung-Der
authors: CD
authors: Hsiao
authors: Su Mei
authors: SM
authors: Wu
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D005880:Gills / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D017210:Tilapia / Q000378:metabolism*
chemical_list: D008112:Liver Glycogen; D002104:Cadmium; D006003:Glycogen