title: Osteoinduction by Ca-P biomaterials implanted into the muscles of mice.
abstract: The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.
authors: Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
authors: Rui-na
authors: RN
authors: Yang
authors: Feng
authors: F
authors: Ye
authors: Li-jia
authors: LJ
authors: Cheng
authors: Jin-jing
authors: JJ
authors: Wang
authors: Xiao-feng
authors: XF
authors: Lu
authors: Yu-jun
authors: YJ
authors: Shi
authors: Hong-song
authors: HS
authors: Fan
authors: Xing-dong
authors: XD
authors: Zhang
authors: Hong
authors: H
authors: Bu
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D002516:Ceramics; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000601:surgery; D016348:Osseointegration; D010012:Osteogenesis
chemical_list: D000880:Anthraquinones; D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C004468:Alizarin Red S