title: Growth inhibition and chromosomal instability of cultured marsupial (opossum) cells after treatment with DNA polymerase α inhibitor.
abstract: The DNA replication mechanism has been well established for eutherian mammals (placental mammals such as humans, mice, and cattle), but not, to date, for metatherian mammals (marsupials such as kangaroos, koalas, and opossums). In this study, we found that dehydroaltenusin, a selective inhibitor of mammalian (eutherian) DNA polymerase α, clearly suppressed the growth of metatherian (opossum and rat kangaroo) cultured cells. In cultured opossum (OK) cells, dehydroaltenusin also suppressed the progression of DNA replication. These results suggest that dehydroaltenusin inhibits metatherian as well as eutherian DNA replication. Dehydroaltenusin treatment of OK cells engendered fluctuations in the numbers of chromosomes in the OK cells as well as inhibition of cell growth and DNA replication. This suggests that partial inhibition of DNA replication by dehydroaltenusin causes chromosomal instability in cultured cells.
authors: Laboratory of Biology Education, Graduate School of Mathematics and Science Education, Tokyo University of Science (RIKADAI), Japan. takemura@rs.kagu.tus.ac.jp
authors: Masaharu
authors: M
authors: Takemura
authors: Tomoko
authors: T
authors: Kazama
authors: Kurumi
authors: K
authors: Sakuma
authors: Yoshiyuki
authors: Y
authors: Mizushina
authors: Teruyoshi
authors: T
authors: Oshima
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D043171:Chromosomal Instability / Q000187:drug effects*; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007614:Macropodidae; D008394:Marsupialia; D051379:Mice; D009893:Opossums; D051381:Rats
chemical_list: D001578:Benzopyrans; D004791:Enzyme Inhibitors; D004256:DNA Polymerase I; C095645:dehydroaltenusin