title: Simple synthesis of 1,3-cyclopentanedione derived probes for labeling sulfenic acid proteins.
abstract: Facile, two-step synthesis and kinetic characterization of new chemical probes for selective labeling of sulfenic acid (-SOH) in proteins are presented. The synthesis route relies on the simple and highly efficient Michael addition of thiol containing tags or linkers to 4-cyclopentene-1,3-dione, the unsaturated derivative of 1,3-cyclopentanedione.
authors: Department of Internal Medicine, Section on Molecular Medicine, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157, USA.
authors: Jiang
authors: J
authors: Qian
authors: Chananat
authors: C
authors: Klomsiri
authors: Marcus W
authors: MW
authors: Wright
authors: S Bruce
authors: SB
authors: King
authors: Allen W
authors: AW
authors: Tsang
authors: Leslie B
authors: LB
authors: Poole
authors: Cristina M
authors: CM
authors: Furdui
mesh_terms: D000818:Animals; D003517:Cyclopentanes / Q000138:chemical synthesis* / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D013194:Staining and Labeling / Q000379:methods; D013434:Sulfenic Acids / Q000737:chemistry* / Q000302:isolation & purification; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D003517:Cyclopentanes; D011506:Proteins; D013434:Sulfenic Acids; D013438:Sulfhydryl Compounds; C017848:4-cyclopentene-1,3-dione