title: Alpha-tubulin (CsTUA) up-regulated during winter dormancy is a low temperature inducible gene in tea [Camellia sinensis (L.) O. Kuntze].
abstract: The present manuscript describes cloning and expression characterization of alpha-tubulin (CsTUA) gene in an evergreen tree tea [Camellia sinensis (L.) O. Kuntze] in response to winter dormancy (WD), abiotic stresses (sodium chloride, polyethylene glycol, and hydrogen peroxide) and plant growth regulators [abscisic acid (ABA), gibberellic acid (GA(3)), indole-3-butyric acid (IBA), and 6-benzylaminopurine (BA)]. CsTUA encoded a putative protein of 449 amino acids with a calculated molecular weight of 49.6 kDa and an isoelectric point (pI) of 5.09. CsTUA shared 76-84 and 90-95% identity at nucleotide and amino acid level, respectively with TUA genes from other plant species. During the period of active growth (PAG), CsTUA showed maximum expression in floral buds as compared to leaf, stem, fruit and root. Though the transcript was not detectable in the younger leaf tissue during the PAG, the expression was induced within 24 h of the low temperature (LT) treatment. The expression was not modulated by the plant growth regulators either in the tissue harvested during PAG or during WD. It was interesting to record that the expression of CsTUA was up-regulated in response to sodium chloride, polyethylene glycol, and hydrogen peroxide. Data has been discussed on the possible role of CsTUA in imparting tolerance to stresses including to LT so that the tea does not exhibit deciduous nature during winters.
authors: Biotechnology Division, Council of Scientific and Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
authors: Asosii
authors: A
authors: Paul
authors: Lakhvir
authors: L
authors: Lal
authors: Paramvir Singh
authors: PS
authors: Ahuja
authors: Sanjay
authors: S
authors: Kumar
mesh_terms: D028241:Camellia sinensis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D003080:Cold Temperature; D035264:Flowers / Q000187:drug effects / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D057445:Plant Dormancy / Q000187:drug effects / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012621:Seasons; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013662:Tea / Q000187:drug effects / Q000235:genetics*; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
chemical_list: D010937:Plant Growth Regulators; D013662:Tea; D014404:Tubulin; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D006861:Hydrogen Peroxide