title: Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status.
abstract: Flow cytometers are probably the most multipurpose laboratory devices available. They can analyse a vast and very diverse range of cell parameters. This technique has left its mark on cancer, human immunodeficiency virus and immunology research, and is indispensable in routine clinical diagnostics. Flow cytometry (FCM) is also a well-known tool for the detection and physiological status assessment of microorganisms in drinking water, marine environments, food and fermentation processes. However, flow cytometers are seldom used in plant pathology, despite FCM's major advantages as both a detection method and a research tool. Potential uses of FCM include the characterization of genome sizes of fungal and oomycete populations, multiplexed pathogen detection and the monitoring of the viability, culturability and gene expression of plant pathogens, and many others. This review provides an overview of the history, advantages and disadvantages of FCM, and focuses on the current applications and future possibilities of FCM in plant pathology.
authors: Plant Sciences Unit, Institute for Agricultural and Fisheries Research, Caritasstraat 21, 9090 Melle, Belgium. liesbet.dhondt@ilvo.vlaanderen.be
authors: Liesbet
authors: L
authors: D'Hondt
authors: Monica
authors: M
authors: Höfte
authors: Erik
authors: E
authors: Van Bockstaele
authors: Leen
authors: L
authors: Leus
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D016681:Genome, Fungal / Q000235:genetics*; D061066:Plant Pathology; D010944:Plants / Q000382:microbiology*