title: Metagenomic comparison of direct and indirect soil DNA extraction approaches.
abstract: Full pyrosequencing runs of both direct-extracted (high yield, low DNA size) and indirect-extracted DNA (low yield, high DNA size) from the same prairie soil show that the sequence distribution of the majority of the metabolic functions and species detected were statistically similar. Although some microbial functions differed at the 95% confidence interval in bootstrap analyses, the overall functional diversity was the same.
authors: Environmental Microbial Genomics, Ecole Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue, 69134 Ecully, France.
authors: Tom O
authors: TO
authors: Delmont
authors: Patrick
authors: P
authors: Robe
authors: Ian
authors: I
authors: Clark
authors: Pascal
authors: P
authors: Simonet
authors: Timothy M
authors: TM
authors: Vogel
mesh_terms: D004247:DNA / Q000032:analysis* / Q000235:genetics*; D016678:Genome; D056186:Metagenomics / Q000379:methods*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D012987:Soil; D004247:DNA