title: Shotgun proteomic analytical approach for studying proteins adsorbed onto liposome surface.
abstract: The knowledge about the interaction between plasma proteins and nanocarriers employed for in vivo delivery is fundamental to understand their biodistribution. Protein adsorption onto nanoparticle surface (protein corona) is strongly affected by vector surface characteristics. In general, the primary interaction is thought to be electrostatic, thus surface charge of carrier is supposed to play a central role in protein adsorption. Because protein corona composition can be critical in modifying the interactive surface that is recognized by cells, characterizing its formation onto lipid particles may serve as a fundamental predictive model for the in vivo efficiency of a lipidic vector. In the present work, protein coronas adsorbed onto three differently charged cationic liposome formulations were compared by a shotgun proteomic approach based on nano-liquid chromatography-high-resolution mass spectrometry. About 130 proteins were identified in each corona, with only small differences between the different cationic liposome formulations. However, this study could be useful for the future controlled design of colloidal drug carriers and possibly in the controlled creation of biocompatible surfaces of other devices that come into contact with proteins into body fluids.
authors: Dipartimento di Chimica, Sapienza Università di Roma, Rome, Italy.
authors: Anna Laura
authors: AL
authors: Capriotti
authors: Giulio
authors: G
authors: Caracciolo
authors: Chiara
authors: C
authors: Cavaliere
authors: Carlo
authors: C
authors: Crescenzi
authors: Daniela
authors: D
authors: Pozzi
authors: Aldo
authors: A
authors: Laganà
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry; D002853:Chromatography, Liquid; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D013058:Mass Spectrometry; D053758:Nanoparticles; D010316:Particle Size; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D001798:Blood Proteins; D008081:Liposomes; D011506:Proteins