title: Selection of irrigation fluid to eradicate free cancer cells during head and neck cancer surgery.
abstract: BACKGROUND
  Free cancer cells can be spilled out from exposed tumors or ruptured tumors. We examined the cytocidal effect of various irrigation fluids on free cancer cells in an animal wound model mimicking head and neck surgery.
  
  
  METHODS
  Cancer cell-contaminated wounds were made with C3H/HeJ mice and syngeneic squamous cell carcinoma (SCC VII) cells. Distilled water, 5% povidone-iodine, 1.5% H(2)O(2), normal saline, and cisplatin were used to irrigate for 5 minutes. In vitro tumor growth assays were done with different concentrations and exposure times of povidone-iodine and distilled water.
  
  
  RESULTS
  In the animal study, povidone-iodine significantly inhibited tumor growth. Povidone-iodine caused substantial inhibition of in vitro tumor growth, even at the concentration of 0.05%. After 30 seconds of exposure to 1% povidone-iodine, cancer cells were completely inhibited.
  
  
  CONCLUSIONS
  Povidone-iodine could be selected preferentially for the irrigation fluid during head and neck surgery, especially when the wound is suspected of cancer cell contamination.
authors: Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul, South Korea. jhunhah@snu.ac.kr
authors: J Hun
authors: JH
authors: Hah
authors: Dong Hwan
authors: DH
authors: Roh
authors: Young Ho
authors: YH
authors: Jung
authors: Kwang Hyun
authors: KH
authors: Kim
authors: Myung-Whun
authors: MW
authors: Sung
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006258:Head and Neck Neoplasms / Q000601:surgery*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007431:Intraoperative Complications / Q000517:prevention & control; D051379:Mice; D008809:Mice, Inbred C3H; D009366:Neoplasm Seeding; D011206:Povidone-Iodine / Q000494:pharmacology*; D011897:Random Allocation; D012680:Sensitivity and Specificity; D007507:Therapeutic Irrigation / Q000379:methods; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D011206:Povidone-Iodine; D006861:Hydrogen Peroxide