title: Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces.
abstract: Strains of gram-negative anaerobic rods were isolated from chinchilla (Chinchilla lanigera) faeces, and three strains, ST161(T), ST33 and ST37(T), were investigated taxonomically. Based on phylogenetic analyses and specific phenotypic characteristics, the three strains were allocated to the genus Bacteroides. Phylogenetic analyses of their 16S rRNA gene sequences revealed that strain ST161(T) formed a distinct line of descent, with highest sequence similarity to strain ST33 (98.7 %) and Bacteroides oleiciplenus JCM 16102(T) (97.7 %). High levels of DNA-DNA relatedness (79-89 %) were found between strains ST161(T) and ST33, but low levels were found between strain ST161(T) and B. oleiciplenus JCM 16102(T) (33-37 %) and between strain ST33 and B. oleiciplenus JCM 16102(T) (33-37 %). These data clearly indicated that strains ST161(T) and ST33 represent a single novel species. 16S rRNA gene sequence analyses showed that strain ST37(T) also formed a distinct line of descent, with highest sequence similarity to Bacteroides acidifaciens JCM 10556(T) (96.5 %) and Bacteroides caccae JCM 9498(T) (95.6 %). Analysis of hsp60 gene sequences also supported these relationships. Based on phenotypic and phylogenetic characteristics, two novel species, Bacteroides stercorirosoris sp. nov. and Bacteroides faecichinchillae sp. nov., are thus proposed. The type strains of B. stercorirosoris and B. faecichinchillae are ST161(T) ( = JCM 17103(T) = CCUG 60872(T)) and ST37(T) ( = JCM 17102(T) = CCUG 60873(T)), respectively. The DNA G+C contents of strains ST161(T) and ST37(T) were 45.7 and 41.0 mol%, respectively.
authors: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors: Maki
authors: M
authors: Kitahara
authors: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors: Mitsuo
authors: M
authors: Sakamoto
authors: Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
authors: Sayaka
authors: S
authors: Tsuchida
authors: Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
authors: Koh
authors: K
authors: Kawasumi
authors: Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
authors: Hiromi
authors: H
authors: Amao
authors: Benno Laboratory, RIKEN Innovation Center, Wako, Saitama 351-0198, Japan.
authors: Yoshimi
authors: Y
authors: Benno
authors: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
authors: Moriya
authors: M
authors: Ohkuma
mesh_terms: D000332:Aerobiosis; D000818:Animals; D015373:Bacterial Typing Techniques; D001439:Bacteroides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002682:Chinchilla / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S