title: Zoledronic acid inhibits vasculogenic mimicry in murine osteosarcoma cell line in vitro.
abstract: BACKGROUND
  To study the effects of zoledronic acid (ZA) on the vasculogenic mimicry of osteosarcoma cells in vitro.
  
  
  METHODS
  A Three-dimensional culture of LM8 osteosarcoma cells on a type I collagen matrix was used to investigate whether osteosarcoma cells can develop vasculogenic mimicry, and to determine the effects of ZA on this process. In addition, the cellular ultrastructural changes were observed using scanning electron microscopy and laser confocal microscopy. The effects of ZA on the translocation of RhoA protein from the cytosol to the membrane in LM8 cells were measured via immunoblotting.
  
  
  RESULTS
  ZA inhibited the development of vasculogenic mimicry by the LM8 osteosarcoma cells, decreased microvilli formation on the cell surface, and disrupted the F-actin cytoskeleton. ZA prevented translocation of RhoA protein from the cytosol to the membrane in LM8 cells.
  
  
  CONCLUSIONS
  ZA can impair RhoA membrane localization in LM8 cells, causing obvious changes in the ultrastructure of osteosarcoma cells and induce cell apoptosis, which may be one of the underlying mechanisms by which the agent inhibits the development of vasculogenic mimicry by the LM8 cells.
authors: Department of Orthopaedics, Huazhong University of Science and Technology, Wuhan, China.
authors: Dehao
authors: D
authors: Fu
authors: Xianfeng
authors: X
authors: He
authors: Shuhua
authors: S
authors: Yang
authors: Weihua
authors: W
authors: Xu
authors: Tao
authors: T
authors: Lin
authors: Xiaobo
authors: X
authors: Feng
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D001859:Bone Neoplasms / Q000098:blood supply* / Q000188:drug therapy* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D004164:Diphosphonates / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology*; D051379:Mice; D008871:Microvilli / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000473:pathology* / Q000517:prevention & control*; D009924:Organ Culture Techniques; D012516:Osteosarcoma / Q000098:blood supply* / Q000188:drug therapy* / Q000648:ultrastructure; D021381:Protein Transport / Q000187:drug effects / Q000502:physiology; D000077211:Zoledronic Acid; D020742:rhoA GTP-Binding Protein / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D020533:Angiogenesis Inhibitors; D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D007093:Imidazoles; D000077211:Zoledronic Acid; D020742:rhoA GTP-Binding Protein