title: Performing the Labeled microRNA pull-down (LAMP) assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs.
abstract: We developed a simple, direct, and cost-effective approach to search for the most likely target genes of a known miRNA in vitro. We term this method "Labeled microRNA (miRNA) pull-down assay system," or LAMP. Briefly, the pre-miRNA is labeled with digoxigenin (DIG), mixed with cell extracts, and immunoprecipitated by anti-DIG antiserum. We concluded that LAMP is an experimental approach for high-throughput identification of the target gene of known miRNAs from both Caenorhabditis elegans and zebrafish (Danio rerio), yielding fewer false-positive results than those produced by using only the bioinformatics approach.
authors: Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan. hsurnai@gmail.com
authors: Ren-Jun
authors: RJ
authors: Hsu
authors: Huai-Jen
authors: HJ
authors: Tsai
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D004076:Digoxigenin / Q000737:chemistry / Q000276:immunology; D057166:High-Throughput Screening Assays / Q000379:methods*; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology; D047468:Immunoprecipitation / Q000379:methods*; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics; D015027:Zebrafish / Q000235:genetics*
chemical_list: D007106:Immune Sera; D035683:MicroRNAs; D012333:RNA, Messenger; D004076:Digoxigenin