title: Microbial utilization and selectivity of pectin fractions with various structures.
abstract: To evaluate the fermentation properties of oligosaccharides derived from pectins and their parent polysaccharides, a 5-ml-working-volume, pH- and temperature-controlled fermentor was tested. Six pectic oligosaccharides representing specific substructures found within pectins were prepared. These consisted of oligogalacturonides (average degrees of polymerization [DP] of 5 and 9), methylated oligogalacturonides (average DP of 5), oligorhamnogalacturonides (average DP of 10 as a disaccharide unit of galacturonic acid and rhamnose), oligogalactosides (average DP of 5), and oligoarabinosides (average DP of 6). The influence of these carbohydrates on the human fecal microbiota was evaluated. Use of neutral sugar fractions resulted in an increase in Bifidobacterium populations and gave higher organic acid yields. The Bacteroides-Prevotella group significantly increased on all oligosaccharides except oligogalacturonides with an average DP of 5. The most selective substrates for bifidobacteria were arabinan, galactan, oligoarabinosides, and oligogalactosides.
authors: Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading, United Kingdom.
authors: Chatchaya
authors: C
authors: Onumpai
authors: Sofia
authors: S
authors: Kolida
authors: Estelle
authors: E
authors: Bonnin
authors: Robert A
authors: RA
authors: Rastall
mesh_terms: D000704:Analysis of Variance; D017360:Arabidopsis / Q000737:chemistry; D058491:Bacterial Load / Q000379:methods; D001439:Bacteroides / Q000378:metabolism; D001644:Bifidobacterium / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology* / Q000592:standards; D005591:Chemical Fractionation / Q000379:methods; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009844:Oligosaccharides / Q000378:metabolism; D010368:Pectins / Q000378:metabolism*; D012210:Rhamnose / Q000378:metabolism; D012639:Seeds / Q000737:chemistry
chemical_list: D005232:Fatty Acids, Volatile; D006603:Hexuronic Acids; D009844:Oligosaccharides; C007819:galacturonic acid; D010368:Pectins; D012210:Rhamnose