title: A simple and rapid nuclear staining method for Rhizoctonia solani Kuhn.
abstract: We developed a modified staining technique using acridine orange to stain the nuclei of Rhizoctonia solani. Acridine orange solution was prepared in acetic acid buffer, pH 7.2. Staining for 15 min was critical for observing the nuclei. All of the isolates were found to be multinucleated. The nuclei appeared bright green with light orange background. This method is simple, rapid and reproducible.
authors: Department of Molecular Biology, Yuvaraja's College, University of Mysore, Mysore, Karnataka, India.
authors: M
authors: M
authors: Seema
authors: B D
authors: BD
authors: Punith
authors: N S
authors: NS
authors: Devaki
mesh_terms: D000165:Acridine Orange; D002467:Cell Nucleus / Q000648:ultrastructure; D005456:Fluorescent Dyes; D010935:Plant Diseases / Q000382:microbiology; D012232:Rhizoctonia / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014026:Nicotiana / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange