title: EspG of enteropathogenic and enterohemorrhagic E. coli binds the Golgi matrix protein GM130 and disrupts the Golgi structure and function.
abstract: The enteric pathogens enteropathogenic Escherichia coli (EPEC), enterohaemorrhagic E. coli (EHEC) and Shigella flexneri all translocate at least one effector protein of the EspG protein family into host cells via a type III secretion system (T3SS). The EspG family comprises EspG, EspG2 and VirA. From a Y2H screen, we identified the Golgi matrix protein GM130 as a potential binding partner of EspG. We confirmed EspG:GM130 protein interaction by affinity co-purification. In co-immunoprecipitation experiments EspG was co-precipitated with GM130 while both GM130 and tubulins were co-precipitated with EspG. When expressed ectopically in HeLa cells, the EspG protein family all localized to the Golgi and induced fragmentation of the Golgi apparatus. All EspG family proteins were also able to disrupt protein secretion to a greater extent than the T3SS effector NleA/EspI, which has previously been shown to localize to the Golgi and interact with SEC24 to disrupt COPII vesicle formation. We hypothesize that EspG:GM130 interaction disrupts protein secretion either through direct disruption of GM130 function or through recruitment of other EspG interacting proteins to the Golgi.
authors: Centre for Molecular Microbiology and Infection, Imperial College, London SW7 2AZ, UK.
authors: Abigail
authors: A
authors: Clements
authors: Katherine
authors: K
authors: Smollett
authors: Sau Fung
authors: SF
authors: Lee
authors: Elizabeth L
authors: EL
authors: Hartland
authors: Martin
authors: M
authors: Lowe
authors: Gad
authors: G
authors: Frankel
mesh_terms: D001324:Autoantigens / Q000378:metabolism*; D054324:Enterohemorrhagic Escherichia coli / Q000378:metabolism*; D054308:Enteropathogenic Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011485:Protein Binding
chemical_list: D001324:Autoantigens; D029968:Escherichia coli Proteins; C429282:EspG protein, E coli; C097741:Golgin subfamily A member 2; D008565:Membrane Proteins