title: Honeybee royal jelly and nobiletin stimulate CRE-mediated transcription in ERK-independent and -dependent fashions, respectively, in PC12D cells.
abstract: To prove the pharmacological actions of honeybee royal jelly (RJ) on the nervous system, we examined the effects of RJ on CRE-mediated transcription. RJ increased CRE-mediated transcription in PC12D cells. Moreover, CRE-mediated transcriptional activity by RJ was enhanced by nobiletin. U0126, a MEK inhibitor, inhibited CRE-mediated transcription by combining RJ plus nobiletin without affecting transcription by RJ alone. These results suggest that RJ stimulates CRE-mediated transcription via an ERK-independent cascade, whereas the increasing CRE-mediated transcriptional effect by nobiletin is dependent on ERK phosphorylation. Combining RJ plus nobiletin may activate effectively neuronal functions via enhancement of CRE-mediated transcription.
authors: Department of Anti-dementia Functional Food Development, Graduate School of Engineering, Tohoku University, Japan.
authors: Hironori
authors: H
authors: Fujiwara
authors: Ako
authors: A
authors: Kogure
authors: Masahiro
authors: M
authors: Sakamoto
authors: Tohru
authors: T
authors: Yamakuni
authors: Yoshihiro
authors: Y
authors: Mimaki
authors: Kiyoshi
authors: K
authors: Murata
authors: Nobuyuki
authors: N
authors: Hitomi
authors: Kikuji
authors: K
authors: Yamaguchi
authors: Yasushi
authors: Y
authors: Ohizumi
mesh_terms: D000818:Animals; D001516:Bees; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005227:Fatty Acids / Q000494:pharmacology*; D047309:Flavones / Q000494:pharmacology*; D020930:MAP Kinase Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009420:Nervous System / Q000187:drug effects / Q000378:metabolism; D016716:PC12 Cells; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*; D000099219:Royal Jelly
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D005227:Fatty Acids; D047309:Flavones; D020930:MAP Kinase Kinase Kinases; D000099219:Royal Jelly; C008661:nobiletin