title: Narrowing down the apricot Plum pox virus resistance locus and comparative analysis with the peach genome syntenic region.
abstract: Sharka disease, caused by the Plum pox virus (PPV), is one of the main limiting factors for stone fruit crops worldwide. Only a few resistance sources have been found in apricot (Prunus armeniaca L.), and most studies have located a major PPV resistance locus (PPVres) on linkage group 1 (LG1). However, the mapping accuracy was not sufficiently reliable and PPVres was predicted within a low confidence interval. In this study, we have constructed two high-density simple sequence repeat (SSR) improved maps with 0.70 and 0.68 markers/cm, corresponding to LG1 of 'Lito' and 'Goldrich' PPV-resistant cultivars, respectively. Using these maps, and excluding genotype-phenotype incongruent individuals, a new binary trait locus (BTL) analysis for PPV resistance was performed, narrowing down the PPVres support intervals to 7.3 and 5.9 cm in 'Lito' and 'Goldrich', respectively. Subsequently, 71 overlapping oligonucleotides (overgo) probes were hybridized against an apricot bacterial artificial chromosome (BAC) library, identifying 870 single BACs from which 340 were anchored onto a map region of approximately 30-40 cm encompassing PPVres. Partial BAC contigs assigned to the two allelic haplotypes (resistant/susceptible) of the PPVres locus were built by high-information content fingerprinting (HICF). In addition, a total of 300 BAC-derived sequences were obtained, and 257 showed significant homology with the peach genome scaffold_1 corresponding to LG1. According to the peach syntenic genome sequence, PPVres was predicted within a region of 2.16 Mb in which a few candidate resistance genes were identified.
authors: Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, Moncada, Valencia, Spain.
authors: Elsa María
authors: EM
authors: Vera Ruiz
authors: José Miguel
authors: JM
authors: Soriano
authors: Carlos
authors: C
authors: Romero
authors: Tetyana
authors: T
authors: Zhebentyayeva
authors: Javier
authors: J
authors: Terol
authors: Elena
authors: E
authors: Zuriaga
authors: Gerardo
authors: G
authors: Llácer
authors: Albert Glenn
authors: AG
authors: Abbott
authors: María Luisa
authors: ML
authors: Badenes
mesh_terms: D002874:Chromosome Mapping; D020090:Chromosome Segregation / Q000235:genetics; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D020451:Contig Mapping; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology; D017801:Plum Pox Virus / Q000276:immunology*; D027861:Prunus / Q000235:genetics* / Q000276:immunology / Q000821:virology*; D026801:Synteny / Q000235:genetics*
chemical_list: D005819:Genetic Markers