title: Multiple approaches to evaluate the toxicity of the biomass fuel cow dung (kanda) smoke.
abstract: Cow dung (Kanda) is a major source of energy in rural and urban population of developing countries and is burnt in traditional open stoves in confined space of kitchen without proper ventilation. In epidemiological studies, biomass fuel smoke has been reported to be responsible for several respiratory disorders in exposed population. In a laboratory experiment, female wistar rats were exposed to kanda smoke for 60 min/day over a period of 12 weeks. Chemical analysis of smoke showed the presence of PAHs. The increase in CYP1A1, GST-ya, GST-yc expression was found in 12 week exposed lung tissues as compared with controls. The exposure to smoke resulted in significant alteration in the BALF cells in the form of clustering of alveolar macrophages and giant cell formation with vacuolated cytoplasm. The macrophages also showed thickness and villi like projections on the cell surface thus reducing their phagocytic activities. Histopathological changes in lung tissue were manifested in the form of damage to bronchiolar epithelium, edema and thickening of alveolar septa and emphysema after 4 and 8 week of exposure. These findings suggest that exposure to kanda smoke increases pulmonary tissue damage and may result in various forms of respiratory infections in the exposed popultion.
authors: Inhalation Toxicology Division, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, PO Box No. 80, Lucknow 226001, UP, India.
authors: Kewal
authors: K
authors: Lal
authors: U
authors: U
authors: Mani
authors: Ruchi
authors: R
authors: Pandey
authors: Nrashant
authors: N
authors: Singh
authors: Abhay K
authors: AK
authors: Singh
authors: D K
authors: DK
authors: Patel
authors: Mahendra P
authors: MP
authors: Singh
authors: R C
authors: RC
authors: Murthy
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000473:pathology*; D008372:Manure; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012906:Smoke / Q000009:adverse effects* / Q000032:analysis; D036401:Toxicity Tests, Chronic
chemical_list: D000393:Air Pollutants; D008372:Manure; D011084:Polycyclic Aromatic Hydrocarbons; D012906:Smoke; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase