title: Highlighting chromosome loops in DNA-picked chromatin (DPC).
abstract: Growing evidence supports the concept that dynamic intra- and inter-chromosomal links between specific loci contribute to the creation of cell-type specific gene expression profiles. Therefore, analysis of the establishment of peculiar functional correlations between sites, also distant on linear DNA, that govern the transcriptional process appears to be of fundamental relevance. We propose here an experimental approach showing that 17β-estradiol-induced transcription associates to formation of loops between the promoter and termination regions of hormone-responsive genes. This strategy reveals as a tool to be also suitably used, in conjunction with automated techniques, for an extensive analysis of sites shared by multiple genes for induced expression.
authors: Dipartimento di Patologia Generale, Seconda Università degli Studi di Napoli; Naples, Italy.
authors: Ciro
authors: C
authors: Abbondanza
authors: Caterina
authors: C
authors: De Rosa
authors: Maria Neve
authors: MN
authors: Ombra
authors: Fabiana
authors: F
authors: Aceto
authors: Nicola
authors: N
authors: Medici
authors: Lucia
authors: L
authors: Altucci
authors: Bruno
authors: B
authors: Moncharmont
authors: Giovanni Alfredo
authors: GA
authors: Puca
authors: Antonio
authors: A
authors: Porcellini
authors: Enrico Vittorio
authors: EV
authors: Avvedimento
authors: Bruno
authors: B
authors: Perillo
mesh_terms: D045744:Cell Line, Tumor; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D002877:Chromosomes, Human / Q000737:chemistry* / Q000235:genetics; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D019254:Genes, bcl-2 / Q000235:genetics; D056466:Histone Demethylases / Q000378:metabolism; D006801:Humans; D009690:Nucleic Acid Conformation; D040901:Proteomics / Q000379:methods*; D020218:Response Elements / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004958:Estradiol; D056466:Histone Demethylases