title: Specific serum IgG, but not IgA, antibody against purified Opisthorchis viverrini antigen associated with hepatobiliary disease and cholangiocarcinoma.
abstract: Opisthorchiasis caused by Opisthorchis viverrini infection induces hepatobiliary disease (HBD)-associated cholangiocarcinoma (CCA) via a chronic inflammatory immune response. Here, we evaluated specific IgG and IgA antibodies against different fractions of O. viverrini antigen in residents from an endemic community in Northeast Thailand with varying hepatobiliary abnormalities. Crude somatic O. viverrini antigen was purified into three fractions (viz., P1, P2 and P3) by gel infiltration chromatography and these served as antigens for detection of fluke-specific IgG and IgA antibodies by enzyme-linked immunosorbent assay (ELISA). The results revealed fluke-specific IgG and IgA antibody levels-against these antigens from subjects with O. viverrini-positive HBD-higher than in subjects with O. viverrini-negative HBD. Interestingly, the rank of fluke-specific IgG (and not IgA) antibody levels against crude extract and P1 antigens was CCA>severe HBD>mild HBD>healthy individuals. Purified antigens reduced cross-reactivity with other parasites compared to the crude antigen. Multiple linear regression analysis showed that HBD status was significantly associated with the liver fluke-specific IgG antibody against purified antigens. These results suggest that purified O. viverrini-antigen improves serodiagnosis for the evaluation of opisthorchiasis-associated HBD, and may be useful in the screening of opisthorchiasis in subjects at risk of developing CCA.
authors: Centre for Research and Development in Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors: Porntip
authors: P
authors: Pinlaor
authors: Porntip
authors: P
authors: Pongsamart
authors: Nuttanan
authors: N
authors: Hongsrichan
authors: Arunnee
authors: A
authors: Sangka
authors: Thitima
authors: T
authors: Srilunchang
authors: Eimorn
authors: E
authors: Mairiang
authors: Paiboon
authors: P
authors: Sithithaworn
authors: Somchai
authors: S
authors: Pinlaor
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D001650:Bile Duct Neoplasms / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging / Q000276:immunology; D001653:Bile Ducts, Intrahepatic / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology; D001659:Biliary Tract / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology; D001660:Biliary Tract Diseases / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging / Q000276:immunology; D018281:Cholangiocarcinoma / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging / Q000276:immunology; D002850:Chromatography, Gel; D006224:Cricetinae; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005399:Fishes / Q000469:parasitology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016014:Linear Models; D008099:Liver / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000150:complications / Q000000981:diagnostic imaging / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000175:diagnosis / Q000276:immunology*; D009891:Opisthorchis / Q000276:immunology*; D013045:Species Specificity; D013785:Thailand; D014463:Ultrasonography
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G