title: Subchondral bone changes in three different canine models of osteoarthritis.
abstract: OBJECTIVE
  To test the hypothesis that changes in subchondral bone are significantly different among three canine models of osteoarthritis (OA).
  
  
  METHODS
  In 21 purpose-bred mongrel dogs, OA was induced in one knee joint via either anterior cruciate ligament transection (ACLt; n = 5), medial femoral condylar groove creation (GR; n = 6), or medial meniscal release (MR; n = 5). Five dogs that had sham surgery (SH; n = 5) in one knee joint served as controls. Lameness scoring was performed every 4 weeks. Twelve weeks after surgery, the knee joints were examined by histology and histomorphometry.
  
  
  RESULTS
  Articular cartilage pathology as determined by Mankin scores was significantly severe in all three OA models compared to SH controls in the medial tibia (P < 0.001 to P = 0.026). ACLt had significantly thinner subchondral plate thickness (Sp.Th) in both the medial and lateral tibias while MR had significantly thicker Sp.Th in the medial tibia compared to SH controls (P < 0.001 to P = 0.011). Trabecular bone volume (BV/TV) and trabecular bone thickness (Tb.Th) for ACLt were significantly less than SH controls in the tibias (P < 0.001 to P = 0.011). Tibial Sp.Th, BV/TV, and Tb.Th were all moderately to strongly correlated with lameness scores obtained throughout the study period (r = -0.436 to r = -0.738, P < 0.001 to P = 0.047) while Mankin scores showed moderate to strong correlations with Sp.Th in each OA model (r = 0.465 to r = 0.816, P < 0.001 to P = 0.033).
  
  
  CONCLUSIONS
  Changes in Sp.Th are associated with articular cartilage damage while tibial Sp.Th and BV/TV and Tb.Th appear to be all influenced by joint loading alterations.
authors: Comparative Orthopaedic Laboratory, University of Missouri, Columbia, MO 65211, USA. kurokik@missouri.edu
authors: K
authors: K
authors: Kuroki
authors: C R
authors: CR
authors: Cook
authors: J L
authors: JL
authors: Cook
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000473:pathology / Q000601:surgery; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006614:Hindlimb / Q000473:pathology; D007794:Lameness, Animal / Q000473:pathology; D008592:Menisci, Tibial / Q000473:pathology / Q000601:surgery; D020370:Osteoarthritis, Knee / Q000473:pathology*; D013977:Tibia / Q000473:pathology*