title: Transgenic zebrafish recapitulating tbx16 gene early developmental expression.
abstract: We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.
authors: Department of Molecular Biosciences, Special Research Centre for the Molecular Genetics of Development, University of Adelaide, Adelaide, South Australia, Australia. simon.wells@adelaide.edu.au
authors: Simon
authors: S
authors: Wells
authors: Svanhild
authors: S
authors: Nornes
authors: Michael
authors: M
authors: Lardelli
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D005453:Fluorescence; D018507:Gene Expression Regulation, Developmental; D016678:Genome / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007395:Interneurons / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020825:T-Box Domain Proteins; D029961:Zebrafish Proteins; C112539:tbx16 protein, zebrafish; D049452:Green Fluorescent Proteins