title: HIV type 1 subtype surveillance in central Kenya.
abstract: Human immunodeficiency virus 1 (HIV-1) infection is characterized by genetic diversity such that specific viral subtypes are predominant in specific geographic areas. To determine circulating subtypes of HIV-1 in different parts of central Kenya, a cross-sectional study was carried out on HIV-1-positive blood samples collected from consenting individuals in eight hospitals of Kenya's central province. Proviral DNA was extracted from peripheral blood mononuclear cells. Polymerase chain reaction and direct sequencing using primers generated from a highly conserved region of HIV-1 env gp41 were carried out. Ninety-six samples were successfully amplified and sequenced. Analysis of the sequences showed that a majority of them belonged to subtype A1 (67/96, 69.8%), followed by subtypes D (18, 18.7%) and C (11/96, 11.5%). Consistent with findings in other parts of Kenya, HIV-1 subtype A1 was the most dominant virus in circulation. Continued surveillance of circulating subtypes of HIV-1 in Kenya is important in determining the evolution of the HIV/AIDS epidemic in Kenya.
authors: Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
authors: Sheila
authors: S
authors: Kageha
authors: Raphael W
authors: RW
authors: Lihana
authors: Vincent
authors: V
authors: Okoth
authors: Matilu
authors: M
authors: Mwau
authors: Fredrick A
authors: FA
authors: Okoth
authors: Elijah M
authors: EM
authors: Songok
authors: Jane M
authors: JM
authors: Ngaira
authors: Samoel A
authors: SA
authors: Khamadi
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D006679:HIV Seropositivity / Q000453:epidemiology* / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007630:Kenya / Q000453:epidemiology; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral