title: Preoperative analysis of 11q loss using circulating tumor-released DNA in serum: a novel diagnostic tool for therapy stratification of neuroblastoma.
abstract: Allelic deletion of the long arm of chromosome 11 (11q loss) is closely associated with the prognosis of neuroblastoma (NB). Here we examined 11q loss using tumor-released DNA fragments in the sera of 24 cases. The allelic intensity score of a panel of polymorphic markers in 11q23 in serum DNA was significantly different between the 11q loss-positive group and the11q loss-negative group. The 11q loss-positive and -negative groups did not overlap when a cut-off value of 0.5 was chosen for the allelic intensity score. Our serum-based 11q loss analysis could predict the allelic status of 11q in tumors.
authors: Department of Pediatrics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors: Shigeki
authors: S
authors: Yagyu
authors: Tomoko
authors: T
authors: Iehara
authors: Takahiro
authors: T
authors: Gotoh
authors: Mitsuru
authors: M
authors: Miyachi
authors: Yoshiki
authors: Y
authors: Katsumi
authors: Ken
authors: K
authors: Kikuchi
authors: Kunihiko
authors: K
authors: Tsuchiya
authors: Shinya
authors: S
authors: Osone
authors: Hiroshi
authors: H
authors: Kuroda
authors: Tohru
authors: T
authors: Sugimoto
authors: Tadashi
authors: T
authors: Sawada
authors: Hajime
authors: H
authors: Hosoi
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002675:Child, Preschool; D002872:Chromosome Deletion; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D004247:DNA / Q000097:blood*; D053938:DNA Fragmentation; D006801:Humans; D007223:Infant; D018895:Microsatellite Repeats / Q000235:genetics; D009447:Neuroblastoma / Q000175:diagnosis* / Q000235:genetics / Q000628:therapy; D011379:Prognosis; D017384:Sequence Deletion
chemical_list: D004247:DNA