title: Rottlerin dissolves pre-formed protein amyloid: a study on hen egg white lysozyme.
abstract: BACKGROUND
  Deposition of protein fibrillar aggregates called amyloids in the tissue, is the principal cause of several degenerative diseases. Here, we have shown the disaggregation potential of rottlerin towards hen egg white lysozyme (HEWL) fibrils formed under alkaline conditions (pH-12.2).
  
  
  METHODS
  Several biophysical methods like Atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and fluorescence emission spectra were used for the study.
  
  
  CONCLUSIONS
  Rottlerin exhibited instantaneous disaggregation effect on HEWL fibrils as monitored by Thioflavin T assay, anisotropy study and AFM imaging. Further we have monitored the conformational changes induced by rottlerin on the fibril in terms of surface hydrophobicity and secondary structure through 8-anilino-1-naphthalene sulfonic acid (ANS) fluorescence and FTIR study respectively. We have also attempted to elucidate the type of interaction between HEWL and rottlerin at pH-12.2 employing techniques like quenching study and FTIR.
  
  
  CONCLUSIONS
  Rottlerin seems to have potential application as anti-amyloid compound.
authors: Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India.
authors: Nandini
authors: N
authors: Sarkar
authors: Manjeet
authors: M
authors: Kumar
authors: Vikash Kumar
authors: VK
authors: Dubey
mesh_terms: D000098:Acetophenones / Q000494:pharmacology*; D000682:Amyloid / Q000737:chemistry / Q000187:drug effects*; D058227:Amyloidogenic Proteins; D001578:Benzopyrans / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D018625:Microscopy, Atomic Force; D009113:Muramidase / Q000737:chemistry*; D011487:Protein Conformation / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000098:Acetophenones; D000682:Amyloid; D058227:Amyloidogenic Proteins; D001578:Benzopyrans; C085746:rottlerin; C421864:hen egg lysozyme; D009113:Muramidase