title: Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry.
abstract: In this study, assisted by affinity-guided DMAP strategy, we developed a novel (19)F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of (19)F nuclei, glycoproteins detected by our (19)F-biosensor are signatured by broadened peaks in (19)F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by (19)F MRI.
authors: Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto, Japan.
authors: Yedi
authors: Y
authors: Sun
authors: Yousuke
authors: Y
authors: Takaoka
authors: Shinya
authors: S
authors: Tsukiji
authors: Michiko
authors: M
authors: Narazaki
authors: Tetsuya
authors: T
authors: Matsuda
authors: Itaru
authors: I
authors: Hamachi
mesh_terms: D015374:Biosensing Techniques; D005462:Fluorine Radioisotopes / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011725:Pyridines / Q000737:chemistry
chemical_list: C417980:4-(dimethylamine)pyridine; D005462:Fluorine Radioisotopes; D006023:Glycoproteins; D037102:Lectins; D011725:Pyridines