title: A microfluidic system for fast detection of mitochondrial DNA deletion.
abstract: This study reports an integrated microfluidic system capable of automatic extraction and analysis of mitochondrial DNA (mtDNA). Mitochondria are the energy production and metabolism centres of human and animal cells, which supply most of the energy for maintaining physiological functions and play an important role in the process of cell death. Because it lacks an effective repair system, mtDNA suffers much higher oxidative damage and usually harbours more mutations than nuclear DNA. Alterations of mtDNA have been reported to be strongly associated with mitochondrial dysfunction, mitochondria-related diseases, aging, and many important human diseases such as diabetes and cancers. Thus, an effective tool for automatic detection of mtDNA deletion is in great need. This study, therefore, proposed a microfluidic system integrating three enabling modules to perform the entire protocol for the detection of mtDNA deletion. Crucial processes which included mtDNA extraction, nucleic acid amplification, separation and detection of the target genes were automatically performed. When compared with traditional assays, the developed microfluidic system consumed fewer samples and reagents, achieved a higher mtDNA extraction rate, and could automate all the processes within a shorter period of time (150 minutes). It may provide a powerful tool for the analysis of mitochondria mutations in the near future.
authors: Department of Engineering Science, National Cheng Kung University, Tainan, 701, Taiwan.
authors: Chen-Min
authors: CM
authors: Chang
authors: Li-Fang
authors: LF
authors: Chiu
authors: Pei-Wen
authors: PW
authors: Wang
authors: Dar-Bin
authors: DB
authors: Shieh
authors: Gwo-Bin
authors: GB
authors: Lee
mesh_terms: D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics*; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D012867:Skin / Q000473:pathology
chemical_list: D004272:DNA, Mitochondrial