title: Anti-lipid peroxidation and induction of apoptosis in the erythroleukaemic cell line K562 by extracts from (Tunisian) Rhamnus alaternus L. (Rhamnaceae).
abstract: Total oligomer flavonoids (TOF) enriched and ethyl acetate (EA) extracts from Rhamnus alaternus induce apoptotic death in human chronic myelogenous leukaemia K562 cell line, as demonstrated by gel electrophoresis, which demonstrates the characteristic ladder patterns of DNA fragmentation and the proteolytic cleavage of poly(ADP ribose) polymerase (PARP). The effect of R. alaternus extract in reducing oxidative stress was evaluated by anti-lipid peroxidation which was monitored by measuring malondialdehyde level in K562 cultured cells. The TOF and EA extracts were found to be effective to protect against lipid peroxidation. Their IC₅₀ values were 196 and 273 µg mL⁻¹, respectively. These findings suggest that R. alaternus extracts exhibit potential antioxidant and proapoptotic properties.
authors: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne, 5019 Monastir, Tunisie.
authors: Rebai Ben
authors: RB
authors: Ammar
authors: Aicha
authors: A
authors: Neffati
authors: Ines
authors: I
authors: Skandrani
authors: Mohamed Ben
authors: MB
authors: Sghaier
authors: Wissem
authors: W
authors: Bhouri
authors: Kamel
authors: K
authors: Ghedira
authors: Leila
authors: L
authors: Chekir-Ghedira
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D031955:Rhamnus / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D008315:Malondialdehyde; D011065:Poly(ADP-ribose) Polymerases