title: Ethanolic extract of fructus Alpinia oxyphylla protects against 6-hydroxydopamine-induced damage of PC12 cells in vitro and dopaminergic neurons in zebrafish.
abstract: In an attempt to understand the neuroprotective effect of Fructus Alpinia oxyphylla (AOE) and to elucidate its underlying mechanism of action, the ethanolic extract of AOE was investigated using zebrafish and PC12 cell models. AOE prevented and restored 6-hydroxydopamine (6-OHDA)-induced dopaminergic (DA) neuron degeneration and attenuated a deficit of locomotor activity in a zebrafish (Danio rerio) model of Parkinson's disease (PD). Treatment with AOE increased the viability of 6-OHDA-treated PC12 cells in vitro in a dose-dependent manner by attenuating cellular apoptosis. However, protocatechuic acid (PCA) and chrysin, two known polyphenol components of AOE, could not reproduce the neuroprotective activity of AOE in the PD zebrafish or PC12 cell models. A mechanistic study found that the protective effect of AOE against 6-OHDA-induced neuronal injury involved anti-inflammatory action (down-regulation of gene expression of IL-1β and TNF-α) and anti-oxidative action (inhibition of NO production and iNOS expression in PC12 cells). Moreover, the PI3K-AKT pathway might be part of the mechanism of neuroprotection of AOE. The results of this research are expected to provide a scientific rationale for the use of AOE in the treatment of PD. However, it is important that the active components that contribute to the neuroprotective action of AOE are identified and characterized.
authors: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Av. Padre Toma's Pereira S.J. Taipa, Macao SAR, China.
authors: Zai-Jun
authors: ZJ
authors: Zhang
authors: Lorita C V
authors: LC
authors: Cheang
authors: Mei-Wei
authors: MW
authors: Wang
authors: Guo-Hui
authors: GH
authors: Li
authors: Ivan K
authors: IK
authors: Chu
authors: Zhi-Xiu
authors: ZX
authors: Lin
authors: Simon M Y
authors: SM
authors: Lee
mesh_terms: D032429:Alpinia; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D019610:Cytoprotection / Q000187:drug effects*; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000502:physiology; D004625:Embryo, Nonmammalian; D000431:Ethanol / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D008124:Locomotion / Q000187:drug effects; D016627:Oxidopamine / Q000633:toxicity*; D016716:PC12 Cells; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development
chemical_list: C442779:Alpinia oxyphylla fruit extract; D010936:Plant Extracts; D000431:Ethanol; D016627:Oxidopamine