title: Proteoliposomes from Mycobacterium smegmatis induce immune cross-reactivity against Mycobacterium tuberculosis antigens in mice.
abstract: Proteoliposomes (PL) obtained from Mycobacterium smegmatis (Ms) were evaluated for their capacity to elicit cross-reactive responses against Mycobacterium tuberculosis (Mtb) antigens in BALB/c mice. Animals immunized with PL adjuvanted with alum (PL-AL) or Freund's Incomplete Adjuvant (PL-IFA) showed significant IgG responses against the PL as well as total Ms lipids. Both groups of animals also showed significant IgG responses against BCG, but only animals immunized with PL-AL produced significant IgG responses against soluble cell wall proteins (SCWP) or whole cell lysate (WCL) of Mtb. Significant DTH responses against WCL were observed in both groups of animals after 24 h, but only PL-AL-immunized mice showed significant DTH responses after 48 h and 72 h. PL-Ms are capable of eliciting cross-reactive humoral and cellular responses against Mtb antigens and thus may be a potential vaccine strategy against tuberculosis.
authors: Instituto Finlay, Ave 27 No. 19805, La Habana, Cuba.
authors: Lissete
authors: L
authors: Rodriguez
authors: Yanelys
authors: Y
authors: Tirado
authors: Fatima
authors: F
authors: Reyes
authors: Alina
authors: A
authors: Puig
authors: Ramlah
authors: R
authors: Kadir
authors: Reinier
authors: R
authors: Borrero
authors: Sonsire
authors: S
authors: Fernandez
authors: Giselle
authors: G
authors: Reyes
authors: Nadine
authors: N
authors: Alvarez
authors: Maria Angeles
authors: MA
authors: Garcia
authors: Maria Elena
authors: ME
authors: Sarmiento
authors: Mohd Nor
authors: MN
authors: Norazmi
authors: Jose Luis
authors: JL
authors: Perez Quinoy
authors: Armando
authors: A
authors: Acosta
mesh_terms: D000276:Adjuvants, Immunologic; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial; D002473:Cell Wall / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology; D020102:Mycobacterium smegmatis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011510:Proteolipids / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology / Q000517:prevention & control; D032581:Tuberculosis Vaccines
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D011510:Proteolipids; D032581:Tuberculosis Vaccines; C020485:proteoliposomes; C072681:Mycobacterium tuberculosis antigens; C041524:aluminum sulfate