title: Attempts to detect West Nile virus in wild birds in Poland.
abstract: The aim of the study was to attempt the detection of West Nile virus (WNV) in wild birds in Poland. Forty-eight species of 1912 wild birds were used for the investigations. The birds were derived from various locations in Poland from early spring till late autumn of the years 2009-2011. The brain samples were homogenised and cellular RNA was isolated. Two methods (RT-PCR and nested RT-PCR) were used. The presence of WNV RNA was not detected in the samples examined. Additionally, a short analysis of the epizootiological situation regarding the presence of WNV in Poland is presented.
authors: National Veterinary Research Institute Puławy, Department of Poultry Viral Diseases, Pulawy, Poland. jowita.niczyporuk@piwet.pulawy.pl
authors: Jowita Samanta
authors: JS
authors: Niczyporuk
authors: Elżbieta
authors: E
authors: Samorek-Salamonowicz
authors: Wojciech
authors: W
authors: Kozdruń
authors: Zbigniew
authors: Z
authors: Mizak
mesh_terms: D000818:Animals; D000835:Animals, Wild; D001715:Bird Diseases / Q000453:epidemiology / Q000821:virology*; D001717:Birds; D011044:Poland / Q000453:epidemiology; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014901:West Nile Fever / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014902:West Nile virus / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral