title: Photoactivation and imaging of optical highlighter fluorescent proteins.
abstract: A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be "turned on" by photoactivation. This unit discusses technical aspects for the general use of photoactivatable fluorescent proteins and introduces some specific applications in the concluding remarks.
authors: Biophotonics Section, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA.
authors: George H
authors: GH
authors: Patterson
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods*; D008164:Luminescent Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques; D010777:Photochemistry / Q000379:methods*
chemical_list: D008164:Luminescent Proteins