title: [Studies on constituents of cultures of fungus Phellinus igniarius].
abstract: OBJECTIVE
  To investigate the chemical constituents of the culture of Phellinus igniarius and their phamacological activities.
  
  
  METHODS
  The constituents were isolated by using a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, and reversed-phase HPLC. Structures of the isolates were identified by spectroscopic data analysis. Cytotoxic, neuroprotective, hepatoprotective, anti-inflammatory, and anti-HIV activities were screened by using cell-based models.
  
  
  RESULTS
  Twenty-nine constituents were isolated. Their structures were identified as three sesquiterpenes: 3S,9R,10S-3-hydroxy-11, 12-O-isopropyldrimene(1), 3S, 9R, 10S-3, 11, 12-trihydroxydrimene (2), and 3S, 4S, 9R, 10S-11, 12, 14-trihydroxydrimene(3); three steriods: 24R-ergosta-4, 6, 8(14), 22-tetraen-3-one (4), stigmasta-7, 22-diene-3b, 5a, 6a-triol (5), and 5a, 8a-epi dioxyergosta-6, 22-diene-3b-ol (6); fourteen cyclo-dipeptide: cyclo (L-Pro-L-Val) (7), cycle (L-Leu-D-Pro) (8), cyclo (L-Leu-L-Pro) (9), cyclo (ILe-Pro) (10), cyclo (Gly-Leu) (11), cyclo (Phe-Ser) (12), cyclo (Ala-Pro) (13), cyclo (Ala-Phe) (14), cyclo (4-HyP-Phe) (15) , cyclo (L-Phe-D-Pro) (16), cyclo (D-Phe-D-Pro) (17), cyclo (6-HyP-Phe) (18), cycle (Gln-Pro) (19), and cycle (Asn-Leu) (20); and nine other compounds: N-acetyl-phenylalanine (21), adenosine (22), phenyldiethanol (23), o-hydroxy-phenylethanol (24), benzoic acid (25), p-methoxybenzoic acid (26), m-methoxybenzoic acid (27), hexadecanoic acid (28), and 3-pyridinecarboxylic acid (29). In the in vitro assays, at a concentration of 1 x 10(-5) mol x L(-1), compounds 5 and 8 showed neuroprotective activity against MPP+ induced PC12-syn cell damage, with a relative cell proliferation rate of 90.3% and 87.5% (P < 0.05). At 1 x 10(-5) mol x L(-1), compounds 12 and 18 showed hepatoprotective activities against DL-galactosamine-induced toxicity examined in WB-F344 cell, with cell survival rates of 25% and 24%, respectivily.
  
  
  CONCLUSIONS
  Compounds 1-29 were obtained from P. igniarius for the first time. Compounds 5 and 8 showed potent PC12-syn protective activities, while 12 and 18 showed hepato cytes (WB-F344 cells) protective activities.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College Beiying 100050, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.forename: Sheng
authors.initials: S
authors.lastname: Lin
authors.forename: Chenggen
authors.initials: C
authors.lastname: Zhu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Yue
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Yang
authors.forename: Jungui
authors.initials: J
authors.lastname: Dai
authors.forename: Jiangong
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry* / Q000254:growth & development*; D049109:Cell Proliferation / Q000187:drug effects; D046508:Culture Techniques; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000032:analysis / Q000494:pharmacology; D009930:Organic Chemicals / Q000032:analysis* / Q000494:pharmacology; D016716:PC12 Cells / Q000187:drug effects; D051381:Rats
chemical_list: D018696:Neuroprotective Agents; D009930:Organic Chemicals