title: [Influence of najanalgesin from Naja naja on GLT-1 in spinal cord of rat in neuropathic pain].
abstract: OBJECTIVE
  To investigate the influence of najanalgesin on glutamate-glial transporter 1(GLT-1) in spinal cord of rats after L5 spinal nerve ligation and transection (SNL), and explore the spinal analgesic mechanism of najanalgesin.
  
  
  METHODS
  One hundred male SD rats were randomly divided into 6 groups: sham(A), SNL(B), SNL + najanalgesin(C), SNL + saline (D), SNL + najanalgesin + liposome (E), SNL + najanalgesin + liposome + GLT-1 As-ODNs(F) and treated with intrathecal injections of 10 p.L saline (A and D), 40 ng X kg(-1) najanalgesin (C, E and F), qd, respectively. Besides intrathecal administration of najanalgesin the rats were intrathecally injected with 10 microL of GLT-1 antisense oligodeoxynucleotides (As-ODNs) (F) and 10 micdroL of liposome(E) once daily on day 3. The L4-L6 segments of the spinal cord were isolated in 1, 4 and 7 d(A,B,C and D), 7 d(E and F) after surgery. The mRNA and protein of GLT-1 were determined.
  
  
  RESULTS
  The SNL model has successfully been set up. Compared to sham group, the expression of GLT-1 mRNA and protein level in group B and D both increased firstly and decreased later, the expression of GLT-1 in group C was significantly increased and kept stable, which were also higher when compared to group D in day 7th. Compared to SNL + najanalgesin group, after intrathecal injection of GLT-1 As-ODNs the GLT-1, expression of GLT-1 in F group significantly decreased. While intrathecal administration of liposome had no significant effect on the spinal GLT-1 expression.
  
  
  CONCLUSIONS
  Najanalgesin could increase the mRNA and protein expression of GLT-1 in spinal cord, which may be one of its spinal mechanisms of analgesia.
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. linjianpepsi@yahoo.com.cn
authors.forename: Qinjian
authors.initials: Q
authors.lastname: Lin
authors.forename: Weijian
authors.initials: W
authors.lastname: Jiang
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Liang
authors.forename: Liping
authors.initials: L
authors.lastname: Han
authors.forename: Cuilan
authors.initials: C
authors.lastname: Zhang
authors.forename: Shujin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000818:Animals; D004546:Elapid Venoms / Q000494:pharmacology*; D017815:Elapidae; D027342:Excitatory Amino Acid Transporter 2 / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009437:Neuralgia / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D004546:Elapid Venoms; D027342:Excitatory Amino Acid Transporter 2; D012333:RNA, Messenger; C543072:najanalgesin