title: The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional.
abstract: Correlative microscopy is a sophisticated approach that combines the capabilities of typically separate, but powerful microscopy platforms: often including, but not limited, to conventional light, confocal and super-resolution microscopy, atomic force microscopy, transmission and scanning electron microscopy, magnetic resonance imaging and micro/nano CT (computed tomography). When targeting rare or specific events within large populations or tissues, correlative microscopy is increasingly being recognized as the method of choice. Furthermore, this multi-modal assimilation of technologies provides complementary and often unique information, such as internal and external spatial, structural, biochemical and biophysical details from the same targeted sample. The development of a continuous stream of cutting-edge applications, probes, preparation methodologies, hardware and software developments will enable realization of the full potential of correlative microscopy.
authors.affiliation: Delaware Biotechnology Institute Bio-Imaging Center, University of Delaware, Newark, DE 19711, United States.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Caplan
authors.forename: Marc
authors.initials: M
authors.lastname: Niethammer
authors.forename: Russell M
authors.initials: RM
authors.lastname: Taylor
authors.forename: Kirk J
authors.initials: KJ
authors.lastname: Czymmek
mesh_terms: D000818:Animals; D002477:Cells / Q000737:chemistry; D055032:Electron Microscope Tomography; D005453:Fluorescence; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D015335:Molecular Probes / Q000737:chemistry; D010083:Oxidants, Photochemical / Q000737:chemistry; D025941:Protein Interaction Mapping / Q000379:methods*; D012984:Software; D014021:Tissue Preservation
chemical_list: D015335:Molecular Probes; D010083:Oxidants, Photochemical