title: Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
abstract: Dendritic cells (DCs) are the most abundant immune cells in the kidney and form an intricate network in the tubulointerstitium, suggesting that they may play an important role in interstitial infections such as pyelonephritis. Here, we optimized a murine pyelonephritis model by instilling uropathogenic Escherichia coli two times at a 3-hour interval, which produced an infection rate of 84%. By 3 hours after the second instillation, resident kidney DCs began secreting the chemokine CXCL2, which recruits neutrophilic granulocytes. During the time studied, DCs remained responsible for most of the CXCL2 production. Neutrophils began infiltrating the kidney 3 hours after the second instillation and phagocytozed bacteria. Macrophages followed 3 hours later and contributed much less to both CXCL2 production and bacterial phagocytosis. To investigate whether DCs recruit neutrophils into the kidney for antibacterial defense, we used CD11c.DTR mice allowing conditional depletion of CD11c(+) dendritic cells. The absence of CD11c(+) DCs markedly delayed neutrophil recruitment and bacterial clearance. In conclusion, these findings suggest that the tubulointerstitial dendritic cell network serves an innate immune sentinel function against bacterial pyelonephritis.
authors.affiliation: Institutes of Molecular Medicine and Experimental Immunology (IMMEI), Rheinische Friedrich-Wilhelms-Universita¨ t,Bonn, Germany.
authors.forename: André P
authors.initials: AP
authors.lastname: Tittel
authors.forename: Christoph
authors.initials: C
authors.lastname: Heuser
authors.forename: Christina
authors.initials: C
authors.lastname: Ohliger
authors.forename: Percy A
authors.initials: PA
authors.lastname: Knolle
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Engel
authors.forename: Christian
authors.initials: C
authors.lastname: Kurts
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D015994:Incidence; D007668:Kidney / Q000166:cytology / Q000382:microbiology / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010586:Phagocytes / Q000166:cytology; D011704:Pyelonephritis / Q000276:immunology* / Q000382:microbiology*; D014551:Urinary Tract / Q000473:pathology; D037521:Virulence Factors
chemical_list: D039521:CD11c Antigen; D037521:Virulence Factors