title: Identification of a MYB3R gene involved in drought, salt and cold stress in wheat (Triticum aestivum L.).
abstract: Abiotic stress seriously affects crop growth and productivity. To better understand the mechanisms plant uses to cope with drought, cold and salt stress, it is necessary to isolate and characterize important regulators response to these stresses. In this study, we cloned a MYB gene from wheat (Triticum aestivum L.) and designated it as TaMYB3R1 based on its conserved three repeats in MYB domain. The sequence of TaMYB3R1 protein shares high identity to other plant MYB3R proteins. Subcellular localization experiment in onion epidermal cells proved that TaMYB3R1 localized in the nucleus. Trans-activation essays in yeast cells confirmed that TaMYB3R1 was a transcriptional activator, and only C-terminal region was able to activate the expression of β-galactosidase. DNA-binding test showed the MSA cis element-binding activity of TaMYB3R1. After exogenous application of phytohormone ABA, the expression of TaMYB3R1 was induced, and its transcripts accumulated up to 24h; this is also the case for MeJA treatment, but after it peaked at 4h, it decreased to low levels. However, either SA or ET had no obvious effect on the expression of TaMYB3R1. Furthermore, the TaMYB3R1 was initially expressed at low levels and was gradually induced following treatment with salt, and continued to increase up to 72 h. This was similar for the cold treatment. In contrast, the peak appeared at 6h of the PEG treatment, and then gradually decreased to low levels. Our results suggest that TaMYB3R1 is potentially involved in wheat response to drought, salt and cold stress.
authors.affiliation: Ministry of Agriculture of R.P. China Key Laboratory of Monitoring and Management of Crop Pathogens and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Cai
authors.forename: Shan
authors.initials: S
authors.lastname: Tian
authors.forename: Changlai
authors.initials: C
authors.lastname: Liu
authors.forename: Hansong
authors.initials: H
authors.lastname: Dong
mesh_terms: D003001:Cloning, Molecular; D003080:Cold Temperature; D019295:Computational Biology; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D012965:Sodium Chloride; D001616:beta-Galactosidase