title: DNA copy number profile discriminates between esophageal adenocarcinoma and squamous cell carcinoma and represents an independent prognostic parameter in esophageal adenocarcinoma.
abstract: We report multiplex ligation-dependent probe amplification analysis (MLPA) of DNA copy number alterations in 59 esophageal cancer samples, stratified by histotype. Results showed that squamous cell carcinoma (SCC) samples present clustered abnormalities with several genes altered at high frequency. Instead, esophageal adenocarcinoma (ADC) samples are characterized by a more widespread genomic instability, and in these patients total DNA copy number alterations resulted to be an independent prognostic factor. The detection of characteristic molecular changes represents a step towards a better understanding of the molecular basis of esophageal tumorigenesis, and might offer the potential for the discovery of tumor-specific biomarkers.
authors.affiliation: Immunology and Molecular Oncology Unit, Veneto Institute of Oncology IOV--IRCCS, Padova, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Rumiato
authors.forename: Giulia
authors.initials: G
authors.lastname: Pasello
authors.forename: Marco
authors.initials: M
authors.lastname: Montagna
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Scaini
authors.forename: Gian Luca
authors.initials: GL
authors.lastname: De Salvo
authors.forename: Anna
authors.initials: A
authors.lastname: Parenti
authors.forename: Matteo
authors.initials: M
authors.lastname: Cagol
authors.forename: Alberto
authors.initials: A
authors.lastname: Ruol
authors.forename: Ermanno
authors.initials: E
authors.lastname: Ancona
authors.forename: Alberto
authors.initials: A
authors.lastname: Amadori
authors.forename: Daniela
authors.initials: D
authors.lastname: Saggioro
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics*; D002869:Chromosome Aberrations; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D003937:Diagnosis, Differential; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D017353:Gene Deletion; D018628:Gene Dosage; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D015345:Oligonucleotide Probes / Q000235:genetics; D011379:Prognosis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: C493966:CDKN2B protein, human; D050762:Cyclin-Dependent Kinase Inhibitor p15; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D042461:Vascular Endothelial Growth Factor A