title: Presence of caffeic acid in flaxseed lignan macromolecule.
abstract: Phenolic compounds were extracted from defatted flaxseeds using ethanol-dioxane (1:1, v/v). The crude extract obtained was purified using Amberlite XAD-16 column chromatography with water and methanol as mobile phases. RP-HPLC and SE-HPLC showed a lignan macromolecule (LM) as a dominant phenolic compound in the purified extract. After the alkaline hydrolysis of LM caffeic acid glucoside (CaAG) was isolated using a semi-preparative HPLC and its structure was confirmed by LC-ESI-MS. In LM of the investigated flaxseed, one molecule of caffeic acid corresponded with five molecules of p-coumaric acid and two molecules of ferulic acid. The presence of caffeic acid in the lignan molecule might be very beneficial due to its high antioxidant activity.
authors.affiliation: Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland. a.kosinska@pan.olsztyn.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kosińska
authors.forename: Kamila
authors.initials: K
authors.lastname: Penkacik
authors.forename: Wiesław
authors.initials: W
authors.lastname: Wiczkowski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Amarowicz
mesh_terms: D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000302:isolation & purification*; D019597:Flax / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification; D046911:Macromolecular Substances / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011108:Polymers; D011422:Propionates; D012639:Seeds / Q000737:chemistry*
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D005960:Glucosides; D017705:Lignans; D046911:Macromolecular Substances; D010936:Plant Extracts; D011108:Polymers; D011422:Propionates; C074337:Amberlite XAD-16; C004999:ferulic acid; C495469:p-coumaric acid; C040048:caffeic acid