title: Effects of Indian herbal formulation Body Revival on human platelet aggregation and myocardial ischemia in rats.
abstract: OBJECTIVE
  To study the effect of Body Revival (BR), a compound traditional Indian herbal medicine, on human platelet aggregation and isoproterenol (IS)-induced myocardial ischemia (MI) damage in male Wistar rats.
  
  
  METHODS
  BR suspension 10, 20 and 30 μg was mixed with platelet-rich plasma and incubated at 37 degrees centigrade for 30 min, respectively. Then, adenosine diphosphate (ADP, 20 mmol/L) or collagen (2 μg) was added in the mixture and the aggregation was observed against platelet-poor plasma mixed with equal volume of suspension of the same test samples. Wistar rats divided into 4 groups were used to investigate BR's effects on IS-induced MI. Levels of serum creatinine kinase (CK), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated by standard commercial biological kits. Serum nitric oxide (NOx) was also measured. The lipid peroxides (LPO) and protein concentrations in heart tissues were measured.
  
  
  RESULTS
  BR could inhibit ADP- or collagen-induced human platelet aggregation dose-dependently. Moreover, it could protect MI caused by IS in rats. BR reduced the levels of serum CK, AST, ALT and NOx dose-dependently and also lowered LPO in heart tissues in comparison with the MI control (P<0.01).
  
  
  CONCLUSIONS
  BR can inhibit human platelet aggregation and protect MI caused by IS in rats.
authors.affiliation: Department of Pharmacology, Institute of Postgraduate Medical Education and Research, West Bengal University of Health Sciences, Kolkata 700020, West Bengal, India.
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Sur
authors.forename: Biswajit
authors.initials: B
authors.lastname: Auddy
authors.forename: Dipankar
authors.initials: D
authors.lastname: Bhattacharyya
mesh_terms: D000818:Animals; D006801:Humans; D008297:Male; D008515:Medicine, Ayurvedic; D017202:Myocardial Ischemia / Q000188:drug therapy*; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000166:cytology*; D010974:Platelet Aggregation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar