title: The influence of cyclomaltooligosaccharides (cyclodextrins) on the enzymatic decomposition of l-phenylalanine catalyzed by phenylalanine ammonia-lyase.
abstract: Cyclomaltohexaose (α-cyclodextrin) and cyclomaltoheptaose (β-cyclodextrin) as well as their four methyl ether derivatives, that is, hexakis(2,3-di-O-methyl)cyclomaltohexaose, hexakis(2,3,6-tri-O-methyl)cyclomaltohexaose, heptakis(2,3-di-O-methyl)cyclomaltoheptaose, and heptakis(2,3,6-tri-O-methyl)cyclomaltoheptaose were investigated as the additives in the course of enzymatic decomposition of l-phenylalanine catalyzed by phenylalanine ammonia-lyase. Only a few of those additives behaved like classical inhibitors of the enzymatic reaction under investigation because the values of the Michaelis constants that were obtained, as well as the maximum velocity values depended mostly atypically on the concentrations of those additives. In most cases cyclodextrins caused mixed inhibition, both competitive and noncompetitive, but they also acted as activators for selected concentrations. This atypical behaviour of cyclodextrins is caused by three different and independent effects. The inhibitory effect of cyclodextrins is connected with the decrease of substrate concentration and unfavourable influence on the flexibility of the enzyme molecules. On the other hand, the activating effect is connected with the decrease of product concentration (the product is an inhibitor of the enzymatic reaction under investigation). All these effects are caused by the ability of the cyclodextrins to form inclusion complexes.
authors.affiliation: Department of Physical Chemistry, Medical University of Warsaw, Banacha 1, Warsaw, Poland. tomasz.gubica@wum.edu.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gubica
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Pełka
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pałka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Temeriusz
authors.forename: Marianna
authors.initials: M
authors.lastname: Kańska
mesh_terms: D003505:Cyclodextrins / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D010649:Phenylalanine / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism*; D047391:alpha-Cyclodextrins / Q000494:pharmacology; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D003505:Cyclodextrins; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; C487420:heptakis(2,3,6-tri-O-methyl)cyclomaltoheptaose; D010649:Phenylalanine; D010650:Phenylalanine Ammonia-Lyase; C032613:alpha-cyclodextrin