title: Amplified ribosomal DNA restriction analysis as a routine tool to assess toxicant driven changes in hindgut bacterial populations of Porcellio dilatatus (Crustacea: Isopoda).
abstract: Changes in saprophytic organism's gut microbial communities may present a threat to organic matter breakdown which can ultimately lead to soil function impairment. In this study, Amplified Ribosomal DNA Restriction Analysis (ARDRA) was evaluated as a potential simple molecular tool to assess shifts in bacterial community structure in hindgut populations of Porcellio dilatatus exposed to contaminated food. This prospective tool can also be used for a variety of purposes and samples prior to the use of more specific and sophisticated methods.
authors.affiliation: CESAM and Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Miguel J G
authors.initials: MJ
authors.lastname: Santos
authors.forename: José Paulo
authors.initials: JP
authors.lastname: Sousa
authors.forename: Igor
authors.initials: I
authors.lastname: Tiago
authors.forename: António
authors.initials: A
authors.lastname: Veríssimo
authors.forename: Marco F L
authors.initials: MF
authors.lastname: Lemos
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D004785:Environmental Pollutants / Q000378:metabolism*; D033321:Isopoda / Q000382:microbiology*; D010575:Pesticides / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015032:Zinc / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D010575:Pesticides; D004262:DNA Restriction Enzymes; D015032:Zinc