title: Implantation of an atelocollagen sponge with autologous bone marrow-derived mesenchymal stromal cells for treatment of vocal fold scarring in a canine model.
abstract: OBJECTIVE
  Vocal fold scarring remains a therapeutic challenge. A new regenerative approach is needed to restore disorganized extracellular matrix. Tissue regeneration requires appropriate cells and a scaffold. Bone marrow-derived mesenchymal stromal cells (BMSCs) are multipotent and secrete many kinds of growth factors to regenerate tissues. Atelocollagen sponges have many large pores that permit cell entry. The present study was performed to evaluate whether implantation of an atelocollagen sponge plus BMSCs is effective for the treatment of vocal fold scarring.
  
  
  METHODS
  Twelve beagles underwent implantation of an atelocollagen sponge or of an atelocollagen sponge with autologous BMSCs (1.0 x 10(6) cells) in the subepithelial pockets of scarred vocal folds. Six months after the operation, vibratory examinations and histologic examinations were performed.
  
  
  RESULTS
  Mucosal vibrations improved significantly for the atelocollagen sponge-implanted vocal folds. Histologic analyses revealed favorable restoration of the extracellular matrix in the lamina propria. Increased distribution of hyaluronic acid and decreased dense collagen deposition were also noted. These improvements were enhanced by implantation of BMSCs.
  
  
  CONCLUSIONS
  Implantation of atelocollagen sponges with autologous BMSCs into scarred vocal folds significantly increased hyaluronic acid distribution and decreased dense collagen deposition in the lamina propria, leading to better mucosal vibration.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kanemaru
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Kitani
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Kojima
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tateya
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000818:Animals; D002921:Cicatrix / Q000473:pathology / Q000601:surgery*; D003094:Collagen; D004195:Disease Models, Animal; D004285:Dogs; D004337:Drug Carriers; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D015503:Surgical Sponges; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014827:Vocal Cords / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D014832:Voice Disorders / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*
chemical_list: D004337:Drug Carriers; C059821:atelocollagen; D003094:Collagen