title: Co-expression of multiple target proteins in plants from a tobacco mosaic virus vector using a combination of homologous and heterologous subgenomic promoters.
abstract: To co-express multiple target proteins, we engineered a single-component chimeric tobacco mosaic virus (TMV)-based vector containing homologous and heterologous capsid protein subgenomic RNA promoters. Delivery of this vector into Nicotiana benthamiana plants via agroinfiltration resulted in co-expression of two reporter genes within a single cell. Furthermore, co-expression of a host-specific antisense RNA or a silencing suppressor protein from this vector augmented the accumulation of green fluorescent protein or a vaccine antigen, hemagglutinin from avian influenza virus A/Vietnam/1194/04. These findings suggest that this chimeric vector utilizing the homologous and heterologous subgenomic TMV promoters has a potential for high-level production of multiple therapeutic proteins including monoclonal antibodies.
authors.affiliation: Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark, DE 19711, USA.
authors.forename: Gourgopal
authors.initials: G
authors.lastname: Roy
authors.forename: Sangeetha
authors.initials: S
authors.lastname: Weisburg
authors.forename: Kelly
authors.initials: K
authors.lastname: Foy
authors.forename: Shailaja
authors.initials: S
authors.lastname: Rabindran
authors.forename: Vadim
authors.initials: V
authors.lastname: Mett
authors.forename: Vidadi
authors.initials: V
authors.lastname: Yusibov
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D011994:Recombinant Proteins