title: [Expression of DOG-1 in gastrointestinal stromal tumor and its diagnostic application].
abstract: OBJECTIVE
  To investigate the expression of DOG-1 in gastrointestinal stromal tumors (GIST) and its diagnostic application.
  
  
  METHODS
  Immunohistochemical EnVision technique was used to assess the expression of DOG-1 in 84 cases of GIST in comparison with CD117 and CD34.
  
  
  RESULTS
  All 84 cases of GIST consisted of variable proportions of spindle and epithelioid tumor cells or just one type of the tumor cell. The expression rates of DOG-1, CD117 and CD34 were 91.3% (42/46), 95.7% (44/46) and 82.6% (38/46), in the group of very low and low risk GIST, and were 100% (38/38), 100% (38/38) and 78.9% (30/38), respectively, in the group of moderate and high risk GIST. True leiomyomas, schwannomas, fibromatosis and normal gastrointestinal mucoca did not express these markers. Moreover, the sensitivity and specificity of DOG-1 in the detection of GIST were similar to those of CD117, without statistical difference (P > 0.05) between the two markers. However, the sensitivity and specificity of DOG-1 detection of moderate and high risk GIST were significantly higher than those of CD34 (P < 0.01).
  
  
  CONCLUSIONS
  DOG-1 is a novel marker of gastrointestinal stromal tumors. It has the sensitivity and specificity higher than CD34, especially in the detection of moderate and high risk GIST. Combined DOG-1 and CD117 immunohistochemistry will likely improve the diagnostic accuracy of GIST.
authors.affiliation: Department of Pathology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610072, China. liuxiang686@yeah.net
authors.forename: Xing-lian
authors.initials: XL
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Dong
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Fang-hua
authors.initials: FH
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000075369:Anoctamin-1; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis; D018118:Chloride Channels; D005260:Female; D046152:Gastrointestinal Stromal Tumors / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D012680:Sensitivity and Specificity
chemical_list: C476213:ANO1 protein, human; D000075369:Anoctamin-1; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D018118:Chloride Channels; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D019009:Proto-Oncogene Proteins c-kit