title: Variability of parvovirus B19 genotype 2 in plasma products with different compositions in the inactivation sensitivity by liquid-heating.
abstract: OBJECTIVE
  Our previous report showed that parvovirus B19 genotype 1 in different solutions derived from plasma preparations showed different heat-sensitivity patterns during liquid-heating. In this study, we similarly examined B19 genotype 2.
  
  
  METHODS
  Two plasma samples one containing B19 genotype 1 and the other genotype 2 DNA were used. Four process samples collected immediately before the heat treatment step in the manufacture of albumin, immunoglobulin, haptoglobin and antithrombin preparations were spiked with B19 and subsequently treated at 60°C for 10 h. A low pH immunoglobulin solution was also spiked with B19 and treated at room temperature for 14 days. Infectivity was then measured.
  
  
  RESULTS
  B19 genotype 2, similar to genotype 1, showed three patterns of inactivation: (i) a rapid inactivation in the albumin and immunoglobulin preparations, (ii) a slow inactivation in the haptoglobin preparation and (iii) only limited inactivation in the antithrombin preparation. Its sensitivity in the low pH immunoglobulin solutions also resembled that of genotype 1.
  
  
  CONCLUSIONS
  Both genotypes 1 and 2 of B19 varied in sensitivity to liquid-heating and low pH among different plasma preparations.
authors.affiliation: Osaka Research Laboratory, Research and Development Division, Benesis Corporation, Osaka, Japan. tsujikawa.muneo@mc.mt-pharma.co.jp
authors.forename: M
authors.initials: M
authors.lastname: Tsujikawa
authors.forename: H
authors.initials: H
authors.lastname: Nishigaki
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: R
authors.initials: R
authors.lastname: Furuki
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Adan-Kubo
authors.forename: Y
authors.initials: Y
authors.lastname: Shimamura
authors.forename: T
authors.initials: T
authors.lastname: Urayama
authors.forename: S
authors.initials: S
authors.lastname: Hattori
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Yunoki
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
mesh_terms: D058731:Blood Safety / Q000379:methods*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006361:Heating; D006358:Hot Temperature; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000494:pharmacology; D008854:Microscopy, Electron; D016732:Parvovirus B19, Human / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010949:Plasma / Q000821:virology*; D038301:Virus Inactivation
chemical_list: D016756:Immunoglobulins, Intravenous