title: Elevated physiological levels of folic acid can increase in vitro growth and invasiveness of prostate cancer cells.
abstract: OBJECTIVE
  • To investigate the effects of different folic acid concentrations on the growth and invasiveness of prostate cancer cell lines. • To determine if observed changes are correlated with changes in levels of the potential prostate cancer biomarker, sarcosine, a byproduct of folate metabolism.
  
  
  METHODS
  • The prostate cancer cell lines PC-3, LNCaP and DU145 were cultured in media containing 4, 20 or 100 nm of folic acid and assayed for growth over 9 days by counting viable cells at 3-day intervals, or for invasion by passage through a Matrigel-coated transwell membrane. • Cells grown in the different folic acid media were collected and subjected to metabolomic analysis by gas chromatography and mass spectrometry to measure levels of intracellular sarcosine.
  
  
  RESULTS
  • The results show that higher levels of folic acid can increase cell growth in PC-3 and LNCaP prostate cancer cell lines, and may also increase the invasive capacity of PC-3, LNCaP and DU145 cells. • We did not observe a correlation between increased invasion from higher folic acid concentrations and levels of sarcosine, but there were significant changes in other metabolites in cells grown in higher levels of folic acid.
  
  
  CONCLUSIONS
  • These findings suggest that folic acid has an important and potentially negative role in prostate cancer progression.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Calgary and Calgary Laboratory Services, Calgary, Alberta, Canada.
authors.forename: Lars F
authors.initials: LF
authors.lastname: Petersen
authors.forename: Nigel T
authors.initials: NT
authors.lastname: Brockton
authors.forename: Ashraf
authors.initials: A
authors.lastname: Bakkar
authors.forename: Shuhong
authors.initials: S
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.forename: Aalim M
authors.initials: AM
authors.lastname: Weljie
authors.forename: Tarek A
authors.initials: TA
authors.lastname: Bismar
mesh_terms: D049109:Cell Proliferation; D005492:Folic Acid / Q000502:physiology*; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000473:pathology*; D012521:Sarcosine / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000737:chemistry
chemical_list: D005492:Folic Acid; D012521:Sarcosine