title: Stimulatory Effect of Autologous Adipose Tissue-Derived Stromal Cells in an Atelocollagen Matrix on Wound Healing in Diabetic db/db Mice.
abstract: We aimed to evaluate the effectiveness of the application of an atelocollagen matrix containing autologous adipose tissue-derived stromal cells (ASCs) on wound healing in diabetic (db/db) mice. Cultured ASCs from db/db mice and from db/+ mice secreted identical amounts of growth factors, cytokines, and type I collagen. ASCs from db/db mice proliferated at the same rate as those from db/+ mice. When DiI-labeled ASCs were applied to full-thickness round skin wounds on the backs of diabetic db/db mice, histological observation at 2 weeks showed that red fluorescent-labeled tissues were formed in the epidermis, dermis, and capillaries. Twelve db/db mice were treated with either matrix alone or matrix containing ASCs and then sacrificed at 1 or 2 weeks. A histological examination demonstrated significantly advanced granulation tissue formation, capillary formation, and epithelialization in those wounds treated with atelocollagen matrix containing ASCs, compared with wounds treated with matrix alone.
authors.affiliation: Department of Plastic and Reconstructive Surgery, National Defense Medical College, Saitama 359-8513, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Nambu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishihara
authors.forename: Satoko
authors.initials: S
authors.lastname: Kishimoto
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yanagibayashi
authors.forename: Naoto
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Azuma
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kanatani
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyosawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno