title: Aurothiomalate as preventive and chain-breaking antioxidant in radical degradation of high-molar-mass hyaluronan.
abstract: The potential anti- or pro-oxidative effects of a disease-modifying antirheumatic drug, aurothiomalate, to protect high-molar-mass hyaluronan against radical degradation were investigated along with L-glutathione - tested in similar functions. Hyaluronan degradation was induced by the oxidative system Cu(II) plus ascorbate known as the Weissberger's oxidative system. The time- and dose-dependent changes of the dynamic viscosity of the hyaluronan solutions were studied by the method of rotational viscometry. Additionally, the antioxidative activity of aurothiomalate expressed as a radical-scavenging capacity based on a decolorization 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay was inspected. At the higher concentrations tested, L-glutathione showed excellent scavenging of (.) OH and peroxyl-type radicals, however, at the lowest concentration applied, its pro-oxidative effect was revealed. The effects of aurothiomalate on hyaluronan degradation were similar to that of L-glutathione, however, at the lowest concentration tested, no significant pro-oxidant effect was observed.
authors.affiliation: Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, SK-84104, Bratislava, Slovakia. Katarina.Valachova@savba.sk
authors.forename: Katarína
authors.initials: K
authors.lastname: Valachová
authors.forename: Andrea
authors.initials: A
authors.lastname: Vargová
authors.forename: Peter
authors.initials: P
authors.lastname: Rapta
authors.forename: Eva
authors.initials: E
authors.lastname: Hrabárová
authors.forename: František
authors.initials: F
authors.lastname: Dráfi
authors.forename: Katarína
authors.initials: K
authors.lastname: Bauerová
authors.forename: Ivo
authors.initials: I
authors.lastname: Juránek
authors.forename: Ladislav
authors.initials: L
authors.lastname: Soltés
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D018501:Antirheumatic Agents / Q000494:pharmacology*; D052160:Benzothiazoles; D002240:Carbohydrate Sequence; D005978:Glutathione / Q000494:pharmacology; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013451:Sulfonic Acids / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018501:Antirheumatic Agents; D052160:Benzothiazoles; D017382:Reactive Oxygen Species; D013451:Sulfonic Acids; D013844:Thiazoles; D006052:Gold Sodium Thiomalate; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006820:Hyaluronic Acid; D005978:Glutathione