title: Crystal structures and repair studies reveal the identity and the base-pairing properties of the UV-induced spore photoproduct DNA lesion.
abstract: UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base-pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.
authors.affiliation: Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Korbinian
authors.initials: K
authors.lastname: Heil
authors.forename: Andrea Christa
authors.initials: AC
authors.lastname: Kneuttinger
authors.forename: Sabine
authors.initials: S
authors.lastname: Schneider
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lischke
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D020029:Base Pairing; D003461:Crystallography; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D019281:Dimerization; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D013058:Mass Spectrometry; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D011506:Proteins; C434331:phosphoramidite; D004247:DNA; C088014:spore photoproduct lyase