title: [The protective effects of SB203580 against mortality and radiation induced intestinal injury of mice].
abstract: This study is to investigate the protective effects of the SB203580 against radiation induced mortality and intestinal injury of mice. A total of 67 male C57BL/6 mice (20.0-22.0 g) were matched according to body weight and randomly assigned to one of three groups: control, total body irradiation exposure (IR, 7.2 Gy) only, and IR (7.2 Gy) + SB203580 (15 mg x kg(-1)). 30 days survival rate was observed in the experiment. In intestinal injury experiment, the expression levels of caspase-3, Ki67, p53 and p-p38 were assayed in the mice intestine crypts. The results showed that the 30 days survival rate was 100% (control), 0 (IR) and 40% (IR+ SB203580), separately. Compared to the IR groups, the positive cells of caspase-3, p53 and p-p38 in crypt cells decreased 33.00%, 21.78% and 34.63%, respectively. The rate of positive cells of Ki67 increased 37.96%. Significant difference was found between all of them (P < 0.01). SB203580 potently protected against radiation-induced lethal and intestinal injury in mice, and it may be a potential radio protector.
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical Collage, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China.
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Chang
authors.forename: Heng
authors.initials: H
authors.lastname: Zhang
authors.forename: Fang-xia
authors.initials: FX
authors.lastname: Guan
authors.forename: Yue-ying
authors.initials: YY
authors.lastname: Wang
authors.forename: De-guan
authors.initials: DG
authors.lastname: Li
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Wu
authors.forename: Cheng-chun
authors.initials: CC
authors.lastname: Wang
authors.forename: Chang-hui
authors.initials: CH
authors.lastname: Zhou
authors.forename: Zhi-bin
authors.initials: ZB
authors.lastname: Zhai
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Hou
authors.forename: Ai-min
authors.initials: AM
authors.lastname: Meng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D053148:Caspase 3 / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000473:pathology; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011725:Pyridines / Q000494:pharmacology*; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011897:Random Allocation; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D014916:Whole-Body Irradiation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D019394:Ki-67 Antigen; D011725:Pyridines; D011837:Radiation-Protective Agents; D016159:Tumor Suppressor Protein p53; D048051:p38 Mitogen-Activated Protein Kinases; C505409:Casp3 protein, mouse; D053148:Caspase 3; C093642:SB 203580