title: Gene expression profile in cultured human umbilical vein endothelial cells exposed to a 300 mT static magnetic field.
abstract: In a previous investigation we reported that exposure to a moderate (300 mT) static magnetic field (SMF) causes transient DNA damage and promotes mitochondrial biogenesis in human umbilical vein endothelial cells (HUVECs). To better understand the response of HUVECs to the 300 mT SMF, a high-quality subtracted cDNA library representative of genes induced in cells after 4 h of static magnetic exposure was constructed. The global gene expression profile showed that several genes were induced after the SMF exposure. The characterized clones are involved in cell metabolism, energy, cell growth/division, transcription, protein synthesis, destination and storage, membrane injury, DNA damage/repair, and oxidative stress response. Quantitative real-time polymerase chain reaction (qRT-PCR) experiments were performed at 4 and 24 h on four selected genes. Their expression profiles suggest that HUVEC's response to SMF exposure is transient. Furthermore, compared to control cells, an up-regulation of several genes involved in cell growth and division was observed. This up-regulation is likely to be the cause of the slight, but significant, increase in cell proliferation at 12 h post-treatment. These results provide additional support to the notion that SMFs may be harmless to human health, and could support the rationale for their possible use in medical treatments.
authors.affiliation: Dipartimento di Scienze Biomolecolari, Università degli Studi di Urbino "Carlo Bo", Italy. emanuela.polidori@uniurb.it
authors.forename: Emanuela
authors.initials: E
authors.lastname: Polidori
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Chiara
authors.initials: C
authors.lastname: Martinelli
authors.forename: Evelin
authors.initials: E
authors.lastname: Colombo
authors.forename: Lucia
authors.initials: L
authors.lastname: Casadei
authors.forename: Deborah
authors.initials: D
authors.lastname: Agostini
authors.forename: Piero
authors.initials: P
authors.lastname: Sestili
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015723:Gene Library; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D060526:Magnetic Fields / Q000009:adverse effects*; D060888:Real-Time Polymerase Chain Reaction; D012449:Safety; D059467:Transcriptome