title: Lipid profiles for intrahepatic cholangiocarcinoma identified using matrix-assisted laser desorption/ionization mass spectrometry.
abstract: BACKGROUND
  We evaluated whether direct tissue matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) analysis of lipids may distinguish intrahepatic cholangiocarcinomas from adjacent normal tissue and from other adenocarcinomas that frequently metastasize to liver.
  
  
  METHODS
  Four pairs of frozen surgical specimens of cholangiocarcinomas and adjacent normal tissue were analyzed using histology-directed, MALDI MS analysis. 2,5-dihydroxybenzoic acid / α-cyano-4-hydroxycinnamic acid were manually deposited on tumor-rich areas, and mass spectra were acquired using a MALDI-time of flight instrument.
  
  
  RESULTS
  Cholangiocarcinomas and adjacent normal tissue samples demonstrated different lipid profiles, as evidenced by permutation P value<0.05 for the cross-validated misclassification rate. Cancer-associated lipid alteration was similar between cholangiocarcinomas and pancreatic cancers, but not between cholangiocarcinomas and colorectal cancers. Baseline lipid profiles were different between cholangiocarcinoma and colorectal cancers.
  
  
  CONCLUSIONS
  MALDI MS analysis of lipid distinguishes cancerous epithelium of cholangiocarcinoma from adjacent normal tissue, and between cholangiocarcinomas and colorectal cancers.
authors.affiliation: Division of Mass Spectrometry Research, Korea Basic Science Institute, Cheongwon, Chungbuk 363-883, Republic of Korea.
authors.forename: Young Seung
authors.initials: YS
authors.lastname: Park
authors.forename: Chong Woo
authors.initials: CW
authors.lastname: Yoo
authors.forename: Seok Cheol
authors.initials: SC
authors.lastname: Lee
authors.forename: Sang Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Jae Hwan
authors.initials: JH
authors.lastname: Oh
authors.forename: Byong Chul
authors.initials: BC
authors.lastname: Yoo
authors.forename: Seung Sam
authors.initials: SS
authors.lastname: Paik
authors.forename: Kyeong Geun
authors.initials: KG
authors.lastname: Lee
authors.forename: So Young
authors.initials: SY
authors.lastname: Jin
authors.forename: Song Cheol
authors.initials: SC
authors.lastname: Kim
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Dongho
authors.initials: D
authors.lastname: Choi
authors.forename: Hark Kyun
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000328:Adult; D001650:Bile Duct Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D001653:Bile Ducts, Intrahepatic / Q000378:metabolism* / Q000473:pathology; D018281:Cholangiocarcinoma / Q000175:diagnosis / Q000378:metabolism*; D005260:Female; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids