title: Wilms' tumor 1-specific cytotoxic T lymphocytes can be expanded from adult donors and cord blood.
abstract: The use of WT1-specific CTL is one potential strategy to treat leukemic relapse following allogeneic stem cell transplant (SCT). Previous studies have largely focused on generating WT1-CTL from adult donors by cloning. We demonstrate that WT1-CTL can be generated from healthy adult donors and from cord blood by stimulating with an overlapping pool of peptides derived from full length WT1 and selecting antigen-specific cells based on the expression of CD137. The rapid expansion with anti-CD3 and IL-2 resulted in a 100-200-fold expansion. These CTL lysed WT1 expressing targets, including leukemia lines, in a HLA restricted manner.
authors.affiliation: Department of Pediatrics, Division of Hematology, Oncology, and Stem Cell Transplantation, Penn State College of Medicine, Hershey, PA 17033, USA. dkolaserikrishnadas@hmc.psu.edu
authors.forename: Deepa K
authors.initials: DK
authors.lastname: Krishnadas
authors.forename: Mindy M
authors.initials: MM
authors.lastname: Stamer
authors.forename: Kimberly
authors.initials: K
authors.lastname: Dunham
authors.forename: Lei
authors.initials: L
authors.lastname: Bao
authors.forename: Kenneth G
authors.initials: KG
authors.lastname: Lucas
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D005240:Feasibility Studies; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D015789:HLA-A2 Antigen / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D016219:Immunotherapy, Adoptive; D007371:Interferon-gamma; D007938:Leukemia / Q000235:genetics / Q000276:immunology* / Q000628:therapy*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014019:Tissue Donors; D014184:Transplantation, Homologous; D025721:WT1 Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D015789:HLA-A2 Antigen; D010446:Peptide Fragments; D025721:WT1 Proteins; D007371:Interferon-gamma