title: Dissection of the functional structure of aptamer17, which specifically recognizes differentiated PC12 cells.
abstract: A specific single-stranded DNA (ssDNA) aptamer (aptamer17) that specifically recognizes differentiated PC12 cells had been previously obtained after 6 rounds of whole cell-based subtractive systematic evolution of ligands by exponential enrichment selection from a random ssDNA library. To further investigate the relationship between the structure and function of this aptamer, 3 truncated ssDNA aptamers were designed according to the predicted secondary structure of aptamer17. Our results show that the stem-loop is the core structure of the aptamers required for specific binding to differentiated PC12 cells, specifically loops I and II. Aptamer17 and the truncated aptamers with this basic structure could bind specifically to differentiated PC12 cells and identify these cells from a mixture of differentiated and undifferentiated PC12 cells. Therefore, truncated forms of aptamer17 may be useful in the clinic to identify undifferentiated and differentiated PC12 cells from a mixture of cells.
authors.affiliation: Department of Stomatology, General Hospital of PLA, Beijing, China.
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Liu
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xi
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Deng
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Ding
authors.forename: Bingfeng
authors.initials: B
authors.lastname: Chu
authors.forename: Dong-Hua
authors.initials: DH
authors.lastname: Su
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D055029:Inverted Repeat Sequences; D009690:Nucleic Acid Conformation; D016716:PC12 Cells; D051381:Rats; D013329:Structure-Activity Relationship
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded