title: Response of human mesenchymal stem cells (hMSCs) to the topographic variation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) films.
abstract: The influence of the topographic morphology of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) films on human mesenchymal stem cells (hMSCs) was investigated in this study. PHBHHx films with various surface characteristics were prepared by compression-molding, solvent-casting  and electrospinning. The adhesion, proliferation and differentiation behaviors of hMSCs were significantly modulated by the surface characteristics of these films. HMSCs could aggregate and form cellular clusters on the cast PHBHHx films, and the time to form cellular aggregates increased  as the surface roughness increased. The aggregated hMSCs on the cast films kept their original surface markers and presented much higher viability during the regular culture and lower differentiation ability upon osteogenic induction than the spread cells on the compression-molded films and  TCPS. HMSCs spread well and showed a specific orientation on the surface of the random electrospun fibrous films, they were not able to migrate into the interior of electrospun fibrous films, and they revealed the highest viability during the regular culture but a lower differentiation activity  upon osteogenic induction. The electrospun fibrous PHBHHx films could serve as a suitable substrate for large quantity culturing of hMSCs when undifferentiated hMSCs are desired.
authors.affiliation: Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan, Taiwan 320, Republic of China.
authors.forename: Bo-Yi
authors.initials: BY
authors.lastname: Yu
authors.forename: Po-Ya
authors.initials: PY
authors.lastname: Chen
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Sun
authors.forename: Yu-Tsang
authors.initials: YT
authors.lastname: Lee
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000737:chemistry* / Q000494:pharmacology*; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002208:Caproates / Q000737:chemistry* / Q000494:pharmacology*; D002449:Cell Aggregation / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects; D012997:Solvents / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D001672:Biocompatible Materials; D002208:Caproates; D012997:Solvents; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates