title: In vitro removal of beta-2-microglobulin from uremic blood with an immunoadsorption wall.
abstract: Dialysis-related amyloidosis (DRA), caused by the accumulation of beta-2-microglobulin (β-2M), remains a major concern in long-term renal replacement therapies. For years, we have developed an immunoadsorption wall (iWall) for the removal of β-2M. In this study, we employed a new approach taking advantage of the melting of a buffer ice rod to improve the conditions associated with the manufacturing of an iWall and tested its performance with uremic serum and blood. The preliminary results reveal that the present iWalls thus prepared not only possess the superior properties of affinity and specificity but also show structural stability and acceptable hemocompatibility. We believe that this breakthrough might provide a promising path to successful treatment of DRA as well as establish a useful platform for studying removal of certain pathological toxins accumulated in the blood.
authors.affiliation: Department of Chemical and Materials Engineering, Cheng Shiu University, Kaohsiung, Taiwan. thyang@csu.edu.tw
authors.forename: Tsung-Hua
authors.initials: TH
authors.lastname: Yang
authors.forename: Bo-Jhou
authors.initials: BJ
authors.lastname: Lin
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Ma
authors.forename: Kang-Ju
authors.initials: KJ
authors.lastname: Chou
mesh_terms: D000686:Amyloidosis / Q000097:blood / Q000209:etiology / Q000517:prevention & control*; D003956:Dialysis / Q000009:adverse effects / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D008422:Materials Testing; D016060:Sorption Detoxification / Q000379:methods*; D014511:Uremia / Q000097:blood* / Q000150:complications; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001613:beta 2-Microglobulin