title: An infectious full-length cDNA clone of potato virus Y(NTN-NW), a recently reported strain of PVY that causes potato tuber necrotic ringspot disease.
abstract: A full-length infectious cDNA clone of potato virus Y (PVY) was constructed based on an isolate of the PVY(NTN-NW) strain, SYR-II-2-8, which is able to cause potato tuber necrotic ringspot disease (PTNRD). Silent point mutations were introduced to modify sequences similar to the prokaryotic promoter elements detected at the 5' terminus of the P3 coding region of SYR-II-2-8. This, along with modification of the growing conditions of E. coli, led to the successful construction of a stable full-length infectious cDNA clone, named p2-8C3. The biological properties of p2-8C3 were identical to those of SYR-II-2-8, both of which induced PTNRD in potato, veinal necrosis in tobacco, and similar symptoms in the potato cultivars inoculated.
authors.affiliation: Laboratory of Plant Pathology, Faculty of Agriculture, Utsunomiya University, Mine-machi 350, Utsunomiya 321-8505, Japan. mhmdsaidsyr@hotmail.com
authors.forename: M
authors.initials: M
authors.lastname: Chikh Ali
authors.forename: A
authors.initials: A
authors.lastname: Said Omar
authors.forename: T
authors.initials: T
authors.lastname: Natsuaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D011198:Solanum tuberosum / Q000821:virology*; D014026:Nicotiana / Q000821:virology
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral