title: Effects of Antiseptics on Pulpal Healing under Calcium Hydroxide Pulp Capping: A Pilot Study.
abstract: OBJECTIVE
  The objective of this pilot study was to evaluate the effects of three different antiseptic materials on healing processes of direct pulp therapies with Ca(OH)(2) histopathologically.
  
  
  METHODS
  Twenty-eight upper and lower first molar teeth from 7 male Wistar rats were used in this study. Four cavities were prepared in each rat in four quadrants, and each quadrant represented different experimental groups. In Group I: 0.5% sodium hypochlorite (NaOCl); in Group II: 2% chlorhexidine digluconate (CHX); in Group III: 0.1% octenidine dihydrochloride (OCT); and in Group IV 0.9% sterile saline was applied to the exposure site with a sterile cotton pellet for 3 minutes. After hemorrhage control, the pulps were capped with hard setting Ca(OH)(2) and, finally, restored with IRM. The animals were euthanized at 21 days post-operatively. After sacrificing, routine histological procedures were performed and evaluated statistically with non-parametric Kruskal-Wallis test among the groups and two-by-two comparisons by using the Mann-Whitney U test for inflammatory response and tissue organization scores at the confidence interval of 95%.
  
  
  RESULTS
  There were significant differences in inflammatory response and tissue organization scores between the groups (P<.05). Statistical evaluation of inflammatory response showed that Group IV was significantly different from Groups I, II and III separately with a higher inflammatory cell response (P<.05) whereas no significant differences were detected between the other groups in two-by-two comparisons (P>.05). Healthy coronal and radicular pulp tissue organization scores indicated that the Group I has better pulp tissue organization than Group IV and this was significantly different (P<.05) whereas no significant differences were observed between the other groups separately (P>.05).
  
  
  CONCLUSIONS
  The antiseptic materials used in this study created an environment that, rather than saline solution, may affect clinical and histological success in a positive way.
authors.affiliation: Department of Paediatric Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Cenkhan
authors.initials: C
authors.lastname: Bal
authors.forename: Alev
authors.initials: A
authors.lastname: Alacam
authors.forename: Tamer
authors.initials: T
authors.lastname: Tuzuner
authors.forename: Resmiye Ebru
authors.initials: RE
authors.lastname: Tirali
authors.forename: Emre
authors.initials: E
authors.lastname: Baris