title: Limitations of spoligotyping and variable-number tandem-repeat typing for molecular tracing of Mycobacterium bovis in a high-diversity setting.
abstract: This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rodriguez-Campos
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.forename: José Luis
authors.initials: JL
authors.lastname: Sáez-Llorente
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.forename: Antonio
authors.initials: A
authors.lastname: Jiménez
authors.forename: Ana
authors.initials: A
authors.lastname: Mateos
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D002161:Camelids, New World; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D014644:Genetic Variation; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D058889:Molecular Typing; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013030:Spain / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D004269:DNA, Bacterial