title: pSEUDO, a genetic integration standard for Lactococcus lactis.
abstract: Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed.
authors.affiliation: Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands.
authors.forename: Joao P C
authors.initials: JP
authors.lastname: Pinto
authors.forename: Araz
authors.initials: A
authors.lastname: Zeyniyev
authors.forename: Harma
authors.initials: H
authors.lastname: Karsens
authors.forename: Hein
authors.initials: H
authors.lastname: Trip
authors.forename: Juke S
authors.initials: JS
authors.lastname: Lolkema
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Jan
authors.initials: J
authors.lastname: Kok
mesh_terms: D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D017930:Genes, Reporter; D005827:Genetics, Microbial / Q000379:methods*; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial