title: Chemical characterization of a procyanidin-rich extract from sorghum bran and its effect on oxidative stress and tumor inhibition in vivo.
abstract: The present study was to characterize a procyanidin-rich extract (PARE) from sorghum ( Sorghum bicolor (L.) Moench) bran and assess its biological activities. The procyanidin oligomers were separated and identified by normal-phase HPLC equipped with fluorescence (FLD) and mass spectrometry (MS) detectors. In addition, the effects of PARE on oxidative stress in mice induced by D-galactose as well as tumor inhibition in C57BL/6J mice bearing Lewis lung cancer were investigated. Administration of D-galactose significantly (p < 0.05) lowered the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). This was accompanied by a significant (p < 0.05) increase in malondialdehyde (MDA) levels in both liver and serum. Administration of PARE (150 mg/kg) significantly (p < 0.05) reversed the d-galactose-induced oxidative stress by enhancing the activities of antioxidant enzymes. Furthermore, PARE administration inhibited tumor growth and metastasis formation by suppressing vascular endothelial growth factor (VEGF) production. The results suggested that PARE had antioxidant and antitumor activities.
authors.affiliation: Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
authors.forename: Peiyou
authors.initials: P
authors.lastname: Qin
authors.forename: Yang
authors.initials: Y
authors.lastname: Yao
authors.forename: Xianjun
authors.initials: X
authors.lastname: Meng
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Zou
authors.forename: Kai
authors.initials: K
authors.lastname: Zhu
authors.forename: Guixing
authors.initials: G
authors.lastname: Ren
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000032:analysis / Q000494:pharmacology*; D018827:Carcinoma, Lewis Lung / Q000737:chemistry / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D044945:Proanthocyanidins / Q000032:analysis* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D045868:Sorghum / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D016588:Anticarcinogenic Agents; D010936:Plant Extracts; D044945:Proanthocyanidins; D042461:Vascular Endothelial Growth Factor A