title: Multihydroxylation of ursolic acid by Pestalotiopsis microspora isolated from the medicinal plant Huperzia serrata.
abstract: The structural modification of ursolic acid by an endophytic fungus Pestalotiopsis microspora, isolated from medicinal plant Huperzia serrata was reported for the first time. The structure diversity was very important for the SAR study of ursolic acid and its derivatives. Incubation of ursolic acid 1 with P. microspora afforded four metabolites: 3-oxo-15α, 30-dihydroxy-urs-12-en-28-oic acid (2), 3β, 15α-dihydroxy-urs-12-en-28-oic acid (3), 3β, 15α, 30- trihydroxy-urs-12-en-28-oic acid (4) and 3,4-seco-ursan-4,30-dihydroxy-12-en-3,28-dioic acid (5). All products were new compounds and their structures elucidation was mainly based on the spectroscopic data.
authors.affiliation: Institute of Medical Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
authors.forename: Shao-bin
authors.initials: SB
authors.lastname: Fu
authors.forename: Jun-shan
authors.initials: JS
authors.lastname: Yang
authors.forename: Jin-long
authors.initials: JL
authors.lastname: Cui
authors.forename: Qing-feng
authors.initials: QF
authors.lastname: Meng
authors.forename: Xu
authors.initials: X
authors.lastname: Feng
authors.forename: Di-An
authors.initials: DA
authors.lastname: Sun
mesh_terms: D001711:Biotransformation; D060026:Endophytes / Q000378:metabolism; D032501:Huperzia / Q000382:microbiology*; D006900:Hydroxylation; D015394:Molecular Structure; D004718:Saccharomycetales / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism*; D013098:Xylariales / Q000302:isolation & purification / Q000378:metabolism*; D000097245:Ursolic Acid
chemical_list: D014315:Triterpenes; D000097245:Ursolic Acid