title: Mechanism of isoproturon resistance in Phalaris minor: in silico design, synthesis and testing of some novel herbicides for regaining sensitivity.
abstract: Isoproturon, 3-p-cumenyl-1 dimethylurea was the only herbicide controlling Phalaris minor, a major weed growing in wheat fields till the early 1980s. Since it has acquired resistance against isoproturon, like other substituted urea herbicides, where the identified target site for isoproturon is in the photosynthetic apparatus at D1 protein of Photosystem-II (PS-II). Nucleotide sequence of susceptible and resistant psbA gene of P. minor has been reported to have four point mutations. During the present work D1 protein of both susceptible and resistant biotypes of P Minor has been modeled. Transmembrane segments of amino acids were predicted by comparing with the nearest homolog of bacterial D1 protein. Volume and area of active site of both susceptible and resistant biotypes has been simulated. Isoproturon was docked at the active site of both, susceptible and resistant D1 proteins. Modeling and simulation of resistance D1 protein indicates that the resistance is due to alteration in secondary structure near the binding site, resulting in loss in cavity area, volume and change in binding position, loss of hydrogen bonds, hydrophobic interaction and complete loss of hydrophobic sites. To regain sensitivity in resistant biotype new derivatives of isoproturon molecules have been proposed, synthesized and tested. Among the 17 derivatives we found that the N-methyl triazole substituted isoproturon is a potential substitute for isoproturon.
authors.affiliation: Indian Institute of Information Technology, Deoghat, Jhalwa, Allahabad 211012, India.
authors.forename: Durg Vijay
authors.initials: DV
authors.lastname: Singh
authors.forename: Kuruba
authors.initials: K
authors.lastname: Adeppa
authors.forename: Krishna
authors.initials: K
authors.lastname: Misra
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004351:Drug Resistance / Q000235:genetics; D006540:Herbicides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D031783:Phalaris / Q000187:drug effects* / Q000235:genetics; D010671:Phenylurea Compounds / Q000494:pharmacology*; D045332:Photosystem II Protein Complex / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D011485:Protein Binding
chemical_list: D006540:Herbicides; D010671:Phenylurea Compounds; D045332:Photosystem II Protein Complex; C028904:isoproturon