title: Inhibitory effects of three oleanolic acid glycosides from Achyranthes japonica on the complement classical pathway.
abstract: The present study evaluated the anticomplement effects of isolated compounds from Achyranthes japonica in the classical pathway of the complement system. Using column chromatography, three compounds: achyranthoside C dimethyl ester, achyranthoside C butyl dimethyl ester, and achyranthoside E dimethyl ester were isolated and evaluated for in vitro anticomplement activity. Achyranthoside C dimethyl ester showed the most potent inhibitory activity against the complement system, with 50% inhibitory concentrations (IC(50)) values of 26.2 μg/mL. This is the first report of anticomplement activity of isolated compounds from A. japonica.
authors.affiliation: Department of Urology, College of Medicine, Dong-A University, Busan, South Korea.
authors.forename: Seil
authors.initials: S
authors.lastname: Jung
authors.forename: Jai-Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
authors.forename: Hyung-In
authors.initials: HI
authors.lastname: Moon
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D000818:Animals; D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D051056:Complement Inactivating Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects* / Q000276:immunology; D006461:Hemolysis / Q000276:immunology; D020128:Inhibitory Concentration 50; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D011817:Rabbits; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep
chemical_list: D051056:Complement Inactivating Agents; D010936:Plant Extracts; D012503:Saponins; D009828:Oleanolic Acid; D002725:Chloroform