title: Detection of DNA-hybridization using a near-field scanning microwave microscope.
abstract: A near-field scanning microwave microscope (NSMM) is used to detect sequence-specific hybridization between surface-immobilized and free DNA single strands. Hybridization between target (free) and capture (immobilized) sequences leads to changes in the reflection coefficient (S11) which are measured by the NSMM. These changes are caused by hybridization-induced modification of the dielectric constant profile of the DNA film. NSMM instrumentation does not require labeling of target sequences with fluorophores or other tagging groups. The physical basis of reflection coefficient changes underpinning the NSMM approach is discussed.
authors.affiliation: Department of Physics and Basic Science Institute for Cell Damage Control, Sogang University, C.P.O. 1142, Seoul 121-742, Republic of Korea.
authors.forename: Seungwan
authors.initials: S
authors.lastname: Kim
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Jang
authors.forename: Songhui
authors.initials: S
authors.lastname: Kim
authors.forename: Tae-Dong
authors.initials: TD
authors.lastname: Kim
authors.forename: Harutyun
authors.initials: H
authors.lastname: Melikyan
authors.forename: Arsen
authors.initials: A
authors.lastname: Babajanyan
authors.forename: Kiejin
authors.initials: K
authors.lastname: Lee
authors.forename: Barry
authors.initials: B
authors.lastname: Friedman
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D008853:Microscopy / Q000295:instrumentation*; D008872:Microwaves; D009693:Nucleic Acid Hybridization
chemical_list: D017931:DNA Primers; D004247:DNA