title: NudC is required for interkinetic nuclear migration and neuronal migration during neocortical development.
abstract: NudC is a highly conserved protein necessary for cytoplasmic dynein-mediated nuclear migration in Aspergillus nidulans. NudC interacts genetically with Aspergillus NudF and physically with its mammalian orthologue Lis1, which is crucial for nuclear and neuronal migration during brain development. To test for related roles for NudC, we performed in utero electroporation into embryonic rat brain of cDNAs encoding shRNAs as well as wild-type and mutant forms of NudC. We show here that NudC, like Lis1, is required for neuronal migration during neocorticogenesis and we identify a specific role in apical nuclear migration in radial glial progenitor cells. These results identify a novel neuronal migration gene with a specific role in interkinetic nuclear migration, consistent with cytoplasmic dynein regulation.
authors.affiliation: Department of Pathology and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA. silvia.cappello@helmholtz-muenchen.de
authors.forename: Silvia
authors.initials: S
authors.lastname: Cappello
authors.forename: Pascale
authors.initials: P
authors.lastname: Monzo
authors.forename: Richard B
authors.initials: RB
authors.lastname: Vallee
mesh_terms: D000818:Animals; D001233:Aspergillus nidulans / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D002552:Cerebral Ventricles / Q000166:cytology / Q000378:metabolism; D005799:Genes, Dominant / Q000235:genetics; D008938:Mitosis; D019579:Neocortex / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D051381:Rats; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D009687:Nuclear Proteins; C094004:Nudc protein, rat