title: Inhibiting effect of bioactive metabolites produced by mushroom cultivation on bacterial quorum sensing-regulated behaviors.
abstract: OBJECTIVE
  This study aimed to search for novel quorum sensing (QS) inhibitors from mushroom and to analyze their inhibitory activity, with a view to their possible use in controlling detrimental infections.
  
  
  METHODS
  The bioactive metabolites produced by mushroom cultivation were tested for their abilities to inhibit QS-regulated behavior. All mushroom strains were cultivated in potato-dextrose medium by large-scale submerged fermentation. The culture supernatant was condensed into 0.2 vol by freeze-drying. The condensed supernatant was sterilized by filtration through a 0.22-μm membrane filter and added to Chromobacterium violaceum CV026 cultures, which were used to monitor QS inhibition. Inhibitory activity was measured by quantifying violacein production using a microplate reader.
  
  
  RESULTS
  The results have revealed that, of 102 mushroom strains, the bioactive metabolites produced by 14 basidiomycetes were found to inhibit violacein production, a QS-regulated behavior in C. violaceum.
  
  
  CONCLUSIONS
  Higher fungi can produce QS-inhibitory compounds.
authors.affiliation: State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Dongying. hzhu @ live.cn
authors.forename: Hu
authors.initials: H
authors.lastname: Zhu
authors.forename: Shou-Xian
authors.initials: SX
authors.lastname: Wang
authors.forename: Shuai-Shuai
authors.initials: SS
authors.lastname: Zhang
authors.forename: Chun-Xu
authors.initials: CX
authors.lastname: Cao
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry; D002861:Chromobacterium / Q000187:drug effects* / Q000502:physiology; D004353:Drug Evaluation, Preclinical; D005658:Fungi / Q000737:chemistry*; D007211:Indoles / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D007211:Indoles; C063155:violacein