title: Sequence variation of the methylene tetrahydrofolate reductase gene (677C>T and 1298 A>C) and traditional risk factors in a South Indian population.
abstract: Methylene tetrahydrofolate reductase (MTHFR) plays a significant role in the metabolism of methionine. MTHFR deficiency is an autosomal recessive trait that could be a significant risk factor for a number of defects, for example, vascular events, due to lower dietary folate intake among South Indians. To find the incidence of 677 C>T and 1298 A>C in MTHFR gene single nucleotide polymorphisms (SNPs) among the south Indian population, polymerase chain reaction and restriction fragment length polymorphism were employed among 152 patients with myocardial infarction and 167 controls. The MTHFR 677CT genotype was found among 35 (22.4%) cases and 08 (4.8%) controls, the MTHFR 677CC allele was found among 115 (73.7%) cases and 159 (94.6%) controls. Also, the analysis of the MTHFR 1298A>C SNP identified the MTHFR 1298CC genotype among 16 (10.3%) cases and 01 (0.6%) control, the MTHFR 1298AC genotype was found in 56 (35.9%) cases and 27 (16.2%) controls, and the MTHFR 1298AA genotype was observed in 80 (51.3%) cases and 139 (82.6%) controls. The C vs. A allele also showed significantly higher frequency among the patients in comparison with the controls (p<0.0001). The results of this study indicate that the MTHFR A1298C SNP is more prevalent among south Indians compared with the MTHFR C677T SNP, suggesting a possible role of MTHFR A1298C in the pathogenesis of heart diseases.
authors.affiliation: Department of Clinical Biochemistry, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, India. dayaphdgenetics@gmail.com
authors.forename: Seetha
authors.initials: S
authors.lastname: Dayakar
authors.forename: Kalal Iravathy
authors.initials: KI
authors.lastname: Goud
authors.forename: Thavanati Parvathi Kumara
authors.initials: TP
authors.lastname: Reddy
authors.forename: Seshagiri P
authors.initials: SP
authors.lastname: Rao
authors.forename: Shyamala B
authors.initials: SB
authors.lastname: Sesikeran
authors.forename: Muralidhar
authors.initials: M
authors.lastname: Sadhnani
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006331:Heart Diseases / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D008754:Methylenetetrahydrofolate Dehydrogenase (NADP)