title: Bell pepper endornavirus: molecular and biological properties, and occurrence in the genus Capsicum.
abstract: Bell peppers (Capsicum annuum) harbour a large dsRNA virus. The linear genome (14.7 kbp) of two isolates from Japanese and USA bell pepper cultivars were completely sequenced and compared. They shared extensive sequence identity and contained a single, long ORF encoding a 4815 aa protein. This polyprotein contained conserved motifs of putative viral methyltransferase (MTR), helicase 1 (Hel-1), UDP-glycosyltransferase and RNA-dependent RNA polymerase. This unique arrangement of conserved domains has not been reported in any of the known endornaviruses. Hence this virus, for which the name Bell pepper endornavirus (BPEV) is proposed, is a distinct species in the genus Endornavirus (family Endornaviridae). The BPEV-encoded polyprotein contains a cysteine-rich region between the MTR and Hel-1 domains, with conserved CXCC motifs shared among several endornaviruses, suggesting an additional functional domain. In agreement with general endornavirus features, BPEV contains a nick in the positive-strand RNA molecule. The virus was detected in all bell pepper cultivars tested and transmitted through seed but not by graft inoculations. Analysis of dsRNA patterns and RT-PCR using degenerate primers revealed putative variants of BPEV, or closely related species, infecting other C. annuum genotypes and three other Capsicum species (C. baccatum, C. chinense and C. frutescens).
authors.affiliation: Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Okada
authors.affiliation: Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Kiyota
authors.affiliation: Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS 39762, USA.
authors.forename: Sead
authors.initials: S
authors.lastname: Sabanadzovic
authors.affiliation: Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Moriyama
authors.affiliation: Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Fukuhara
authors.affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Saha
authors.affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73402, USA.
authors.forename: Marilyn J
authors.initials: MJ
authors.lastname: Roossinck
authors.affiliation: Laboratoire de Physiologie Vegetale, Universite de Cocody-Abidjan, UFR Biosciences, 22 BP, 582 Abidjan 22, Côte d'Ivoire.
authors.forename: Ake
authors.initials: A
authors.lastname: Severin
authors.affiliation: Department of Plant Pathology & Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Valverde
mesh_terms: D020816:Amino Acid Motifs; D002212:Capsicum / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D007564:Japan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014481:United States; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins