title: Nanostructured films as a novel substrate for chondrocytes growth.
abstract: We fabricated a large area silica nano-particle monolayer on glass substrates for the cell growth by the Langmuir-Blodgett technique. A thin film of 300 nm sized mono-dispersed silica particles was constructed on the air-water interface and transferred onto a glass substrate. Chondrocytes were cultured on nano-structured substrates and bare glass substrates for 8 days. The characterizations of chondrocytes on nano-structured substrate were conducted on 3rd and 6th day using confocal laser microscopy and with MTT assay for 8 days. The chondrocytes cultured on nano-structured substrate showed podia like spike and their size was larger than that formed on bare glass substrate. The metabolic activity of chondrocytes on nano-structured substrate was lower than that on bare glass substrate at early-stage, but it was recovered after 4 days.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
authors.forename: Hyo-Sop
authors.initials: HS
authors.lastname: Kim
authors.forename: Woo Hee
authors.initials: WH
authors.lastname: Choi
authors.forename: Young A
authors.initials: YA
authors.lastname: Song
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Byoung-Hyun
authors.initials: BH
authors.lastname: Min
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Kim
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D006801:Humans; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures