title: Proteomic analysis of laser microdissected ovarian cancer tissue with SELDI-TOF MS.
abstract: The evolution of ovarian cancer will highly depend on platinum sensitivity or resistance. In an attempt to better understand this mechanism of resistance, we combined laser microdissection (LMD) of ovarian tumor cells with mass spectrometric techniques. To obtain disease-specific markers we isolated ovarian tumor cells with LMD without contamination of surrounding cells. Proteins were extracted by chemical lysis and subsequent peptide and protein information was gathered using surface-enhanced laser desorption/ionization-time of flight mass spectrometry.
authors.affiliation: Division of Gynecological Oncology, Department of Obstetrics and Gynecology, University Hospitals Leuven, Katholieke Universiteit Leuven, Leuven, Belgium. Isabelle.Cadron@uz.kuleuven.ac.be
authors.forename: Isabelle
authors.initials: I
authors.lastname: Cadron
authors.forename: Toon
authors.initials: T
authors.lastname: Van Gorp
authors.forename: Philippe
authors.initials: P
authors.lastname: Moerman
authors.forename: Etienne
authors.initials: E
authors.lastname: Waelkens
authors.forename: Ignace
authors.initials: I
authors.lastname: Vergote
mesh_terms: D015925:Cryopreservation / Q000379:methods; D005260:Female; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008867:Microtomy / Q000379:methods; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D020543:Proteome