title: In vitro cultures of Drosera aliciae as a source of a cytotoxic naphthoquinone: ramentaceone.
abstract: A protocol for the in vitro propagation of Drosera aliciae to increase the yield of the naphthoquinone, ramentaceone, was developed. The highest micropropagation coefficient was obtained using half-strength Murashige-Skoog medium supplemented with 0.4 μM 6-benzyladenine (BA). The genetic fidelity and stability of the regenerated plants was confirmed with RAPD markers. The activity of the isolated ramentaceone was determined against four human tumor cell lines: U937, HeLa, MCF-7, HCT-116 with the highest cytotoxic activity towards the leukemic U937 cell line with an IC(50) value of 3.2 μM.
authors.affiliation: Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, Gdansk, Poland. kawiak@biotech.ug.gda.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Kawiak
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Królicka
authors.forename: Ewa
authors.initials: E
authors.lastname: Łojkowska
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism* / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry; D018744:DNA, Plant / Q000235:genetics; D029787:Drosera / Q000254:growth & development* / Q000378:metabolism*; D042822:Genomic Instability; D005838:Genotype; D006801:Humans; D020128:Inhibitory Concentration 50; D058889:Molecular Typing; D009285:Naphthoquinones / Q000378:metabolism* / Q000494:pharmacology; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D018744:DNA, Plant; D009285:Naphthoquinones