title: Calcium-sensing receptor activating phosphorylation of PKCδ translocation on mitochondria to induce cardiomyocyte apoptosis during ischemia/reperfusion.
abstract: The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) that activates intracellular effectors; for example, it causes inositol phosphate (IP) and 1,2 diacylglycerol (DAG) accumulation, stimulating the release of intracellular calcium and the activation of the protein kinase Cs (PKCs). The activation of CaSR by ischemia/reperfusion (I/R) induces cardiomyocyte apoptosis through the mitochondrial apoptotic pathway; however, the underlying mechanisms remain unclear. In this study, rat hearts were subjected to 30 min of ischemia followed by 2 h of reperfusion in the presence of a CaSR activator, GdCl(3). Our results revealed that, under these conditions, the expression of CaSR was increased, the number of apoptotic cardiomyocytes was significantly increased (as shown by terminal deoxy-nucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay) and the cells with a typical apoptotic morphology were observed using transmission electron microscopy. Our data further showed that mitochondria isolated from hearts that had undergone I/R combined with GdCl(3) exhibited a significant increase in the translocation of phosphorylated PKCδ to the mitochondria, an increase in cytochrome c (cyt c) release from the mitochondria and a marked decrease in mitochondrial potential. The administration of rottlerin, an inhibitor of PKCδ, significantly reduced reperfusion-induced apoptosis, phospho-PKCδ translocation to the mitochondria and the release of cyt c from the mitochondria. Thus, the involvement of CaSR in cardiac apoptosis through the mitochondrial pathway during I/R with GdCl(3) is related to phospho-PKCδ translocation to the mitochondria.
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150086, China.
authors.forename: Huishuang
authors.initials: H
authors.lastname: Zheng
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.forename: Fanghao
authors.initials: F
authors.lastname: Lu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhanfeng
authors.initials: Z
authors.lastname: Jin
authors.forename: Huan
authors.initials: H
authors.lastname: Ren
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Leng
authors.forename: Jing
authors.initials: J
authors.lastname: Jia
authors.forename: Guangxia
authors.initials: G
authors.lastname: Hu
authors.forename: Shiyun
authors.initials: S
authors.lastname: Dong
authors.forename: Xin
authors.initials: X
authors.lastname: Zhong
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Li
authors.forename: Baofeng
authors.initials: B
authors.lastname: Yang
authors.forename: Changqing
authors.initials: C
authors.lastname: Xu
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D042541:Intracellular Space / Q000378:metabolism; D008297:Male; D018613:Microscopy, Confocal; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000648:ultrastructure; D015428:Myocardial Reperfusion Injury / Q000201:enzymology* / Q000473:pathology*; D009206:Myocardium / Q000201:enzymology / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology* / Q000648:ultrastructure; D010766:Phosphorylation; D051745:Protein Kinase C-delta / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D044169:Receptors, Calcium-Sensing / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D044169:Receptors, Calcium-Sensing; C473605:extracellular calcium cation-sensing receptor, rat; D045304:Cytochromes c; C497260:Prkcd protein, rat; D051745:Protein Kinase C-delta; D002118:Calcium