title: Transgenic sugar beet tolerant to imidazolinone obtained by Agrobacterium-mediated transformation.
abstract: Sugar beet is highly sensitive to imidazolinone herbicides thus rotational restrictions exist. In order to develop imidazolinone tolerant sugar beets als gene from Arabidopsis thaliana encoding acetolactate synthase with S653N mutation was used for genetic transformation. Transgenic sugar beet plants were obtained by Agrobacterium-mediated transformation of aseptic seedlings using vacuum-infiltration. The efficiency of genetic transformation was 5.8%. RT-PCR analysis of obtained plants revealed accumulation of specific als transcript. The resistance to imidazolinone was proved for developed transgenic sugar beet plants in vitro and in greenhouse conditions after spraying with imazethapyr (Pursuit, BASF).
authors.affiliation: Institute of Cell Biology & Genetic Engeneering Natl. Acad. Sci. of Ukraine, Kyiv. iicb@iicb.kiev.ua
authors.forename: E M
authors.initials: EM
authors.lastname: Kishchenko
authors.forename: I K
authors.initials: IK
authors.lastname: Komarnitskii
authors.forename: N V
authors.initials: NV
authors.lastname: Kuchuk
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D027461:Beta vulgaris / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D053209:Herbicide Resistance; D006540:Herbicides / Q000737:chemistry / Q000494:pharmacology*; D048288:Imidazolines / Q000737:chemistry / Q000494:pharmacology*; D009539:Nicotinic Acids / Q000737:chemistry / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014170:Transformation, Genetic
chemical_list: D006540:Herbicides; D048288:Imidazolines; D009539:Nicotinic Acids; C093630:imazethapyr