title: Expression and characterization of c-Myb in prenatal odontogenesis.
abstract: The transcription factor c-Myb is involved in the control of cell proliferation, survival and differentiation. As these processes accompany the morphogenesis of developing teeth, this work investigates the possible role of c-Myb during odontogenesis. Analysis of the expression of c-Myb in the monophyodont mouse was followed by similar analysis in a diphyodont species, the pig, which has a dentition more closely resembling that of the human. The distribution of c-Myb was correlated with the pattern of proliferation and apoptosis and the tooth phenotype of c-Myb mutant mice was also assessed. In the mouse, c-Myb expression was detected throughout prenatal development of the first molar tooth. Negative temporospatial correlation was found between c-Myb expression and apoptosis, while c-Myb expression positively correlated with proliferation. c-Myb-positive cells, however, were more abundant than the proliferating cell nuclear antigen positive cells, suggesting other roles of c-Myb in odontogenesis. In the minipig, in contrast to the mouse, there was an asymmetrical arrangement of c-Myb positive cells, with a higher presence on the labial side of the tooth germ and dental lamina. A cluster of negative cells was situated in the mesenchyme close to the tooth bud. At later stages, the number of positive cells decreased and these cells were situated in the upper part of the dental papilla in the areas of future cusp formation. The expression of c-Myb in both species was strong in the odontoblasts and ameloblasts at the stage of dentin and enamel production suggesting a possible novel role of c-Myb during tooth mineralization.
authors.affiliation: Institute of Animal Physiology and Genetics, v.v.i., Academy of Sciences of the Czech Republic. matalova@iach.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Matalová
authors.forename: Marcela
authors.initials: M
authors.lastname: Buchtová
authors.forename: Abigail S
authors.initials: AS
authors.lastname: Tucker
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Bender
authors.forename: Eva
authors.initials: E
authors.lastname: Janečková
authors.forename: Vlasta
authors.initials: V
authors.lastname: Lungová
authors.forename: Simona
authors.initials: S
authors.lastname: Balková
authors.forename: Jan
authors.initials: J
authors.lastname: Smarda
mesh_terms: D000483:Alleles; D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D003001:Cloning, Molecular; D003817:Dentition; D004622:Embryo, Mammalian / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D009805:Odontogenesis; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature; D014070:Tooth / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C499306:Mybl2 protein, mouse; D018809:Proliferating Cell Nuclear Antigen; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators