title: Evaluation of CD103 (αEβ7) integrin expression by CD8 T cells in blood as a surrogate marker to predict cervical T cell responses in the female genital tract during HIV infection.
abstract: Mucosal homing receptors expressed by blood T cells may be useful surrogates for measuring mucosal T cell immune responses at the site of HIV transmission. Here, we investigated whether HIV-specific responses by T cells expressing the mucosal integrin receptor CD103 in blood reliably predicted corresponding HIV-specific responses at the cervix. The frequency of CD8+ T cells expressing CD103 in blood correlated significantly with the number of CD103+CD8+ T cells from the cervix suggesting that CD103 was involved in trafficking of T cells from blood to the cervical mucosa. TGF-β concentrations in plasma were significantly associated with the frequency of CD103 expression by blood but not cervical CD8 T cells. The majority of Gag-responsive CD8 T cells were CD103+ in both blood and at the cervix. Despite this, the magnitude of Gag-specific IFN-γ responses by CD103+CD8+ T cells in blood did not predict similar Gag-specific responses at the cervix.
authors.affiliation: Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Observatory, South Africa.
authors.forename: Agano
authors.initials: A
authors.lastname: Kiravu
authors.forename: Pam
authors.initials: P
authors.lastname: Gumbi
authors.forename: Nonhlanhla N
authors.initials: NN
authors.lastname: Mkhize
authors.forename: Abraham
authors.initials: A
authors.lastname: Olivier
authors.forename: Lynnette
authors.initials: L
authors.lastname: Denny
authors.forename: Jo-Ann
authors.initials: JA
authors.lastname: Passmore
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D015415:Biomarkers; D001826:Body Fluids / Q000737:chemistry / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002584:Cervix Uteri / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D016207:Cytokines / Q000032:analysis / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation; D015683:Gene Products, gag / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000635:transmission; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007156:Immunologic Memory; D039001:Integrin alpha Chains / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D007371:Interferon-gamma / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D009928:Organ Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000097:blood; D019562:Viral Load
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D016207:Cytokines; D015683:Gene Products, gag; D039001:Integrin alpha Chains; D053773:Transforming Growth Factor beta1; C091367:alpha E integrins; D007371:Interferon-gamma