title: Effects of D-allose on the endocytic activity of dendritic cells and the subsequent stimulation of T cells.
abstract: We examined the effects of a rare sugar, D-allose, which is 6-carbon monosaccharide, on endocytosis and T cell stimulation by dendritic cells (DCs). The endocytosis of BCG-anti-BCG immune complexes by DCs markedly decreased in D-allose-containing medium. Co-culture with T cells (mixed leukocyte reaction, MLR) of DCs, which had been exposed to BCG in D-allose-supplemented medium, induced apoptosis of CD4(+) T cells in a manner dependent on D-allose concentration. After the MLR, DCs cultured in the medium with D-allose expressed less CD40 and more Fas ligands than those cultured without D-allose. It was suggested that the functions of DCs, internalization, processing and the subsequent antigen presentation to T cells, are down-regulated via the action of d-allose.
authors.affiliation: Department of Inflammation Pathology, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan. tasue@med.kagawa-u.ac.jp
authors.forename: Sumiko
authors.initials: S
authors.lastname: Tanaka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology / Q000378:metabolism; D001500:BCG Vaccine / Q000276:immunology / Q000493:pharmacokinetics; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects* / Q000276:immunology; D053222:Fas Ligand Protein / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D005947:Glucose / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism
chemical_list: D051940:B7-2 Antigen; D001500:BCG Vaccine; D019013:CD40 Antigens; D053222:Fas Ligand Protein; D019014:fas Receptor; C002055:allose; D005947:Glucose