title: HPLC separation of all aldopentoses and aldohexoses on an anion-exchange stationary phase prepared from polystyrene-based copolymer and diamine: the effect of NaOH eluent concentration.
abstract: To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D₆ stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pK(a) values, but also the chemical structures of the cyclic aldoses.
authors.affiliation: Department of Human Environmental Sciences, Ochanomizu University, 2-1-1 Otuka, Bunkyo-ku, Tokyo 112-0012, Japan.
authors.forename: Kadumi
authors.initials: K
authors.lastname: Inoue
authors.forename: Kei-ichi
authors.initials: K
authors.lastname: Kitahara
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Aikawa
authors.forename: Sadao
authors.initials: S
authors.lastname: Arai
authors.forename: Takako
authors.initials: T
authors.lastname: Masuda-Hanada
mesh_terms: D001089:Arabinose / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003959:Diamines / Q000737:chemistry*; D005690:Galactose / Q000302:isolation & purification; D005947:Glucose / Q000302:isolation & purification; D006601:Hexoses / Q000302:isolation & purification*; D008358:Mannose / Q000302:isolation & purification; D010429:Pentoses / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D012266:Ribose / Q000302:isolation & purification; D014994:Xylose / Q000302:isolation & purification
chemical_list: D003959:Diamines; D006601:Hexoses; D010429:Pentoses; D011108:Polymers; D011137:Polystyrenes; C057406:gulose; C010997:idose; C002055:allose; C043333:lyxose; D012266:Ribose; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D008358:Mannose; D005690:Galactose