title: Role of SNX16 in the dynamics of tubulo-cisternal membrane domains of late endosomes.
abstract: In this paper, we report that the PX domain-containing protein SNX16, a member of the sorting nexin family, is associated with late endosome membranes. We find that SNX16 is selectively enriched on tubulo-cisternal elements of this membrane system, whose highly dynamic properties and formation depend on intact microtubules. By contrast, SNX16 was not found on vacuolar elements that typically contain LBPA, and thus presumably correspond to multivesicular endosomes. We conclude that SNX16, together with its partner phosphoinositide, define a highly dynamic subset of late endosomal membranes, supporting the notion that late endosomes are organized in distinct morphological and functional regions. Our data also indicate that SNX16 is involved in tubule formation and cholesterol transport as well as trafficking of the tetraspanin CD81, suggesting that the protein plays a role in the regulation of late endosome membrane dynamics.
authors.affiliation: Department of Biochemistry, University of Geneva, Geneva, Switzerland.
authors.forename: Ben
authors.initials: B
authors.lastname: Brankatschk
authors.forename: Véronique
authors.initials: V
authors.lastname: Pons
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.forename: Jean
authors.initials: J
authors.lastname: Gruenberg
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D020126:Brefeldin A / Q000494:pharmacology; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism; D058105:Polymerization / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D058305:Sorting Nexins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D060225:Tetraspanin 28; D016707:Tissue Fixation
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; D058305:Sorting Nexins; D060225:Tetraspanin 28; D020126:Brefeldin A; D002784:Cholesterol