title: Cloning of a vacuolar H(+)-pyrophosphatase gene from the halophyte Suaeda corniculata whose heterologous overexpression improves salt, saline-alkali and drought tolerance in Arabidopsis.
abstract: Salt, saline-alkali conditions, and drought are major environmental factors limiting plant growth and productivity. The vacuolar H(+)-translocating inorganic pyrophosphatase (V-H(+)-PPase) is an electrogenic proton pump that translocates protons into vacuoles in plant cells. Expression of V-H(+)-PPase increases in plants under a number of abiotic stresses, and is thought to have an important role in adaptation to abiotic stress. In this work, we report the isolation and characterization of the gene, ScVP, encoding a vacuolar inorganic pyrophosphatase (V-H(+)-PPase) from the halophyte, Suaeda corniculata. Semi-quantitative reverse transcription-polymerase chain reaction analysis showed that ScVP was induced in roots, stems and leaves under treatment with salt, saline-alkali and drought. Compared with wild-type (WT) Arabidopsis, transgenic plants overexpressing ScVP accumulated more Na(+) in leaves and roots, and showed increased tolerance to high salinity, saline-alkali and drought stresses. The germination percentage of transgenic Arabidopsis seeds was higher than that of WT seeds under the abiotic stresses. The root length of transgenic plants under salt stress was longer than that of WT plants. Furthermore, the rate of water loss during drought stress was higher in WT than in transgenic plants. Collectively, these results indicate that ScVP plays an important role in plant tolerance to salt, saline-alkali and drought stress.
authors.affiliation: Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Dong
authors.forename: Xiu-Duo
authors.initials: XD
authors.lastname: Fan
authors.forename: Xiu-Ming
authors.initials: XM
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Li
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D000468:Alkalies / Q000494:pharmacology*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D019663:Chenopodiaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D043564:Inorganic Pyrophosphatase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055051:Salt-Tolerant Plants / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D020539:Sequence Analysis, Protein; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects / Q000201:enzymology; D014867:Water / Q000378:metabolism
chemical_list: D000468:Alkalies; D018076:DNA, Complementary; D012333:RNA, Messenger; D014867:Water; D012965:Sodium Chloride; D012964:Sodium; D043564:Inorganic Pyrophosphatase