title: Proteomic profiling of two maize inbreds during early gibberella ear rot infection.
abstract: Fusarium graminearum is the causal agent of gibberella ear rot in maize ears, resulting in yield losses due to mouldy and mycotoxin-contaminated grain. This study represents a global proteomic approach to document the early infection by F. graminearum of two maize inbreds, B73 and CO441, which differ in disease susceptibility. Mock- and F. graminearum-treated developing kernels were sampled 48 h post-inoculation over three field seasons. Infected B73 kernels consistently contained higher concentrations of the mycotoxin deoxynivalenol than the kernels of the more tolerant inbred CO441. A total of 2067 maize proteins were identified in the iTRAQ analysis of extracted kernel proteins at a 99% confidence level. A subset of 878 proteins was identified in at least two biological replicates and exhibited statistically significantly altered expression between treatments and/or the two inbred lines of which 96 proteins exhibited changes in abundance >1.5-fold in at least one of the treatments. Many proteins associated with the defense response were more abundant after infection, including PR-10 (PR, pathogenesis-related), chitinases, xylanase inhibitors, proteinase inhibitors, and a class III peroxidase. Kernels of the tolerant inbred CO441 contained higher levels of these defense-related proteins than B73 kernels even after mock treatment, suggesting that these proteins may provide a basal defense against Fusarium infection in CO441.
authors.affiliation: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON, Canada.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Valar
authors.initials: V
authors.lastname: Anoop
authors.forename: Steve
authors.initials: S
authors.lastname: Gleddie
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Harris
mesh_terms: D000465:Algorithms; D053898:Biosynthetic Pathways; D030562:Databases, Protein; D060467:Disease Resistance; D005670:Fusarium / Q000472:pathogenicity*; D005874:Gibberella / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006813:Humidity; D013058:Mass Spectrometry; D054883:Oxylipins / Q000737:chemistry; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology; D040901:Proteomics / Q000379:methods*; D013696:Temperature; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry* / Q000276:immunology / Q000382:microbiology
chemical_list: D054883:Oxylipins; D010940:Plant Proteins; D014255:Trichothecenes; C007262:deoxynivalenol