title: [Apoptosis and expression of Bcl-2 and caspase-3 in ciclosporin-induced gingival overgrowth of rats].
abstract: OBJECTIVE
  To observe the effect of Ciclosporin (CsP) on apoptosis and expression of the associated protein Bcl-2, Caspase-3 in gingival epithelium of rats in order to approach the mechanism of CsP-induced gingival epithelium overgrowth.
  
  
  METHODS
  Eighty SPF grade male 7-week-old Wistar rats were randomly divided into experimental group and control group, and each group was divided into 4 subgroups according to the duration of treatment (10, 20, 30 and 40 days). The experimental objects were given fresh milk including CsP intragastrically and the control ones were given only fresh milk. After perfusion of 4% paraform for internal fixation, the specimens' bucco-lingual paraffin sections at lower first molar were made. Apoptosis was detected using TdT-mediated dUT nick end labeling (TUNEL) and the expression of Bcl-2 and Caspase-3 using immunohistochemisty of PV. The apoptotic index, positive cell rate of Caspase-3 and average gray scale of Bcl-2 was measured with an image analysis system. Data were analyzed by two-way analysis of variance of factorial design.
  
  
  RESULTS
  The apoptosis index and positive cell rate of Caspase-3 were down-regulated in the experimental group, and were significant difference from the control group (P < 0.05). The average gray scale of Bcl-2 was up-regulated in the experimental group, and was significant difference from the control group (P < 0.05).
  
  
  CONCLUSIONS
  CsP-induced gingival epithelial overgrowth is likely to associated with interference to the path of mitochondrial apoptosis and inhibition apoptosis.
authors.affiliation: Dept. of Periodontology, College of Stomatology, Harbin Medical University, Harbin 150001, China.
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Liu
authors.forename: Su
authors.initials: S
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3; D016572:Cyclosporine; D019214:Gingival Overgrowth; D020287:In Situ Nick-End Labeling; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2; D051381:Rats; D017208:Rats, Wistar
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D016572:Cyclosporine; D053148:Caspase 3