title: Optimum induction of recombinant thymidine phosphorylase and its application.
abstract: Recombinant E. coli pDEOA was constructed and lactose can be used instead of IPTG to induce the expression of thymidine phosphorylase by pDEOA. The use of lactose at concentrations higher than 0.5 mmol/L had an induction effect similar to that of IPTG but resulted in a longer initial induction time and better cell growth. The thymidine phosphorylase induced by lactose was very stable at 50°C. Intact pDEOA cells induced by lactose can be used as a source of thymidine phosphorylase. Under standard reaction conditions, several deoxynucleosides were effectively produced from thymidine.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, ECUST, China, Shanghai, PR China.
authors.forename: Qingbao
authors.initials: Q
authors.lastname: Ding
authors.forename: Ling
authors.initials: L
authors.lastname: Ou
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Wei
mesh_terms: D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D007544:Isopropyl Thiogalactoside / Q000378:metabolism / Q000494:pharmacology; D007785:Lactose / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013939:Thymidine Phosphorylase / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D007544:Isopropyl Thiogalactoside; D013939:Thymidine Phosphorylase; D007785:Lactose