title: The utility of an isolated mitochondrial fraction in the preparation of liposomes for the specific delivery of bioactives to mitochondria in live mammalian cells.
abstract: OBJECTIVE
  To develop and evaluate liposomal formulations prepared with an isolated mitochondrial fraction as a mitochondria-specific delivery vehicle.
  
  
  METHODS
  Liposomes were prepared with either a crude mitochondrial fraction extracted from cells or lipids extracted from the crude mitochondrial fraction and were then characterized by determining their size and zeta potential. The cell uptake of the liposomes and loaded bioactive was studied using flow cytometry and confocal microscopy. The cytotoxicity of the formulations was tested by MTS cytotoxicity assay.
  
  
  RESULTS
  Liposomes prepared with the mitochondrial extracts were non-toxic and colocalized with mitochondria in F98 cells. Addition of DOTAP to the liposomes facilitated DNA complexation and the DNA delivered intracellularly co-localized with mitochondria.
  
  
  CONCLUSIONS
  The results from this study establish the potential of using a mitochondrial fraction isolated from cells to prepare liposomes capable of delivering biologically active molecules to mitochondria of live mammalian cells.
authors.affiliation: Department of Pharmaceutical Sciences, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, Boston, Massachusetts 02115, USA.
authors.forename: Mayura A
authors.initials: MA
authors.lastname: Wagle
authors.forename: Laura E
authors.initials: LE
authors.lastname: Martinville
authors.forename: Gerard G M
authors.initials: GG
authors.lastname: D'Souza
mesh_terms: D000818:Animals; D001688:Biological Products / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems; D005079:Excipients; D006801:Humans; D008081:Liposomes / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D008322:Mammals; D008928:Mitochondria / Q000737:chemistry* / Q000378:metabolism; D058990:Molecular Targeted Therapy; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats
chemical_list: D001688:Biological Products; D004337:Drug Carriers; D005079:Excipients; D008081:Liposomes; D004364:Pharmaceutical Preparations; D010743:Phospholipids; D004247:DNA