title: Generation and characterization of a panel of anti-phosphoprotein monoclonal antibodies directed against Mokola virus.
abstract: The generation of a new panel of 21 monoclonal antibodies (MAbs) reactive with the P protein of Mokola virus (MOKV) is described. Through competitive ELISA and immunoblotting analyses, these MAbs were classified into several groups. Consistent with prior studies on lyssavirus P protein antigenic structure, many of the sites recognized by these Mabs appear to correspond to sites identified previously. Studies on the reactivity of these anti-MOKV P MAbs against a collection of lyssaviruses identified MAbs that were broadly cross-reactive to all genus members and others that bound selectively to members of different species. In particular the utility of this MAb panel for differentiation of African lyssaviruses was explored. Such a panel will be useful for further examination of the extent of functional complementation between lyssavirus P proteins.
authors.affiliation: Rabies Centre of Expertise, Ottawa Laboratory (Fallowfield), Canadian Food Inspection Agency, 3851 Fallowfield Rd., Ottawa, Ontario, Canada K2H 8P9. susan.nadin-davis@inspection.gc.ca
authors.forename: Susan A
authors.initials: SA
authors.lastname: Nadin-Davis
authors.forename: Lindsay
authors.initials: L
authors.lastname: Elmgren
authors.forename: Mary
authors.initials: M
authors.lastname: Sheen
authors.forename: Claude
authors.initials: C
authors.lastname: Sabeta
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Wandeler
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D018114:Lyssavirus / Q000145:classification / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000276:immunology*; D012703:Serotyping / Q000379:methods; D014764:Viral Proteins / Q000276:immunology*; D014773:Virology / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D010750:Phosphoproteins; D014764:Viral Proteins