title: Determination of the N-glycosylation patterns of seed proteins: applications to determine the authenticity and substantial equivalence of genetically modified (GM) crops.
abstract: Methods have been developed to determine the N-glycosylation pattern of proteins at the single-seed level in two different biological systems. These were the well-characterized and widely consumed storage protein phaseolin from several species of Phaseolus (bean) and the α-amylase inhibitor from the same Phaseolus species expressed transgenically in pea. The N-glycosylation pattern of the α-amylase inhibitor expressed transgenically in pea was different from that of the inhibitor present in common bean (P. vulgaris), the species of origin of the gene. However, multivariate analysis showed that the differences in N-glycan patterns between the α-amylase inhibitors from common bean and pea were less than those between the inhibitors from common bean and two related bean species, lima bean (Phaseolus lunatus) and tepary bean (Phaseolus acutifolius).
authors.affiliation: Rothamsted Research, Harpenden, Hertfordshire, United Kingdom.
authors.forename: Justin T
authors.initials: JT
authors.lastname: Marsh
authors.forename: Theodora
authors.initials: T
authors.lastname: Tryfona
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Powers
authors.forename: Elaine
authors.initials: E
authors.lastname: Stephens
authors.forename: Paul
authors.initials: P
authors.lastname: Dupree
authors.forename: Peter R
authors.initials: PR
authors.lastname: Shewry
authors.forename: Alison
authors.initials: A
authors.lastname: Lovegrove
mesh_terms: D006031:Glycosylation; D018532:Pisum sativum / Q000737:chemistry / Q000235:genetics; D037121:Plant Lectins / Q000737:chemistry / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000145:classification*; D012639:Seeds / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D037121:Plant Lectins; D010940:Plant Proteins; C061510:alpha-amylase inhibitor, Phaseolus vulgaris; C048826:phaseolin protein, Phaseolus vulgaris