title: [Resolution of optical isomers of beta-amino ketone compounds with two chiral centres by high performance liquid chromatography].
abstract: A high performance liquid chromatographic method was developed for the resolution of optical isomers of beta-amino ketone compounds containing two chiral centres. An AE. Lichrom AM 1 chiral chromatographic column (250 mm x 4.6 mm, 5 microm) was used. The influences o the mobile phase polarity, flow rate and elution mode were investigated. The optimum conditions were the use of hexane-isopropyl alcohol as the mobile phase with a flow rate of 1 mL/min and gradient elution. Under the above chromatographic conditions, the optical isomers of four kinds beta-amino ketone compounds containing two chiral centres were baseline separated (Rs > 1.5). It was proved that the method is fast and easy to separate the optical isomers of beta-amino ketone compounds containing two chiral centres.
authors.affiliation: Key Laboratory of Macromolecular Science of Shaanxi Province, School of Chemistry & Materials Science, Shaanxi Normal University, Xi' an 710062, China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Li
authors.forename: Youpingi
authors.initials: Y
authors.lastname: Tian
authors.forename: Liren
authors.initials: L
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007097:Imines / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D007097:Imines; D007659:Ketones