title: The heat-resistant agglutinin family includes a novel adhesin from enteroaggregative Escherichia coli strain 60A.
abstract: Heat-resistant agglutinin 1 (Hra1) is an accessory colonization factor of enteroaggregative Escherichia coli (EAEC) strain 042. Tia, a close homolog of Hra1, is an invasin and adhesin that has been described in enterotoxigenic E. coli. We devised a PCR-restriction fragment length polymorphism screen for the associated genes and found that they occur among 55 (36.7%) of the enteroaggregative E. coli isolates screened, as well as lower proportions of enterotoxigenic, enteropathogenic, enterohemorrhagic, and commensal E. coli isolates. Overall, 25%, 8%, and 3% of 150 EAEC strains harbored hra1 alone, tia alone, or both genes, respectively. One EAEC isolate, 60A, produced an amplicon with a unique restriction profile, distinct from those of hra1 and tia. We cloned and sequenced the full-length agglutinin gene from strain 60A and have designated it hra2. The hra2 gene was not detected in any of 257 diarrheagenic E. coli isolates in our collection but is present in the genome of Salmonella enterica serovar Heidelberg strain SL476. The cloned hra2 gene from strain 60A, which encodes a predicted amino acid sequence that is 64% identical to that of Hra1 and 68% identical to that of Tia, was sufficient to confer adherence on E. coli K-12. We constructed an hra2 deletion mutant of EAEC strain 60A. The mutant was deficient in adherence but not autoaggregation or invasion, pointing to a functional distinction from the autoagglutinin Hra1 and the Tia invasin. Hra1, Tia, and the novel accessory adhesin Hra2 are members of a family of integral outer membrane proteins that confer different colonization-associated phenotypes.
authors.affiliation: Department of Biology, Haverford College, Haverford, PA 19041, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Mancini
authors.forename: Brooke
authors.initials: B
authors.lastname: Weckselblatt
authors.forename: Yoonjie K
authors.initials: YK
authors.lastname: Chung
authors.forename: Julia C
authors.initials: JC
authors.lastname: Durante
authors.forename: Steven
authors.initials: S
authors.lastname: Andelman
authors.forename: Jessica
authors.initials: J
authors.lastname: Glaubman
authors.forename: Justin D
authors.initials: JD
authors.lastname: Dorff
authors.forename: Samhita
authors.initials: S
authors.lastname: Bhargava
authors.forename: Rebeccah S
authors.initials: RS
authors.lastname: Lijek
authors.forename: Katherine P
authors.initials: KP
authors.lastname: Unger
authors.forename: Iruka N
authors.initials: IN
authors.lastname: Okeke
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism*; D000373:Agglutinins / Q000235:genetics / Q000378:metabolism*; D001422:Bacterial Adhesion; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D048168:Escherichia coli K12 / Q000235:genetics / Q000472:pathogenicity; D017353:Gene Deletion; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D019779:Salmonella enterica / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018830:Adhesins, Escherichia coli; D000373:Agglutinins; D004269:DNA, Bacterial