title: Proteome reference map of Lactobacillus acidophilus NCFM and quantitative proteomics towards understanding the prebiotic action of lactitol.
abstract: Lactobacillus acidophilus NCFM is a probiotic bacterium adapted to survive in the gastrointestinal tract and with potential health benefits to the host. Lactitol is a synthetic sugar alcohol used as a sugar replacement in low calorie foods and selectively stimulating growth of L. acidophilus NCFM. In the present study the whole-cell extract proteome of L. acidophilus NCFM grown on glucose until late exponential phase was resolved by 2-DE (pH 3-7). A total of 275 unique proteins assigned to various physiological processes were identified from 650 spots. Differential 2-DE (DIGE) (pH 4-7) of L. acidophilus NCFM grown on glucose and lactitol, revealed 68 spots with modified relative intensity. Thirty-two unique proteins were identified in 41 of these spots changing 1.6-12.7-fold in relative abundance by adaptation of L. acidophilus NCFM to growth on lactitol. These proteins included β-galactosidase small subunit, galactokinase, galactose-1-phosphate uridylyltransferase and UDP-glucose-4-epimerase, which all are potentially involved in lactitol metabolism. This first comprehensive proteome analysis of L. acidophilus NCFM provides insights into protein abundance changes elicited by the prebiotic lactitol.
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Kgs. Lyngby, Denmark.
authors.forename: Avishek
authors.initials: A
authors.lastname: Majumder
authors.forename: Abida
authors.initials: A
authors.lastname: Sultan
authors.forename: Rosa R
authors.initials: RR
authors.lastname: Jersie-Christensen
authors.forename: Morten
authors.initials: M
authors.lastname: Ejby
authors.forename: Bjarne Gregers
authors.initials: BG
authors.lastname: Schmidt
authors.forename: Sampo J
authors.initials: SJ
authors.lastname: Lahtinen
authors.forename: Susanne
authors.initials: S
authors.lastname: Jacobsen
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005947:Glucose / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013402:Sugar Alcohols / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013402:Sugar Alcohols; D005947:Glucose; C014635:lactitol