title: Highly efficient microscale purification of glycerophospholipids by microfluidic cell lysis and lipid extraction for lipidomics profiling.
abstract: This article presents a novel method for small-scale lipidomics of bacterial cells by integrating extraction of glycerophospholipids on a microchip with a nanoelectrospray ionization quadrupole time-of-flight tandem mass spectrometer (nanoESI-Q-TOF MS/MS). The standard starting point for typical macroscale lipid analysis is a multiphase liquid-liquid extraction. Working with small populations of cells (1 to about 1000) requires a scaled down process in order to minimize dilution and facilitate the interface with microscale separation methods for sample cleanup and introduction to mass spectrometry. We have developed a microfluidic system that allows for lysis of bacterial cells, capture of lipids, and elution of captured lipids from a solid phase for microscale purification of lipids. The best on-chip extraction efficiency for glycerophospholipids was as high as 83.3% by integrating silica beads as the packing material with methanol as the eluent. A total of 10 successive measurements were evaluated indicating that the microchip packed with fresh silica beads is capable of being reused four times without any loss in the lipid extraction process. The initial screening based on high-resolution tandem mass spectrometry data along with a discovery profiling approach revealed the presence of 173 identified phospholipid species from microfluidic cell extracts. This work demonstrates the potential of incorporating microchip-based lipid extraction into cellular lipidomics research.
authors.affiliation: Department of Chemistry and Biochemistry, Duquesne University, Pittsburgh, Pennsylvania 15282, USA. sunt@duq.edu
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Sean
authors.initials: S
authors.lastname: Pawlowski
authors.forename: Mitchell E
authors.initials: ME
authors.lastname: Johnson
mesh_terms: D002167:Campylobacter / Q000737:chemistry; D020404:Glycerophospholipids / Q000032:analysis* / Q000302:isolation & purification; D059625:Liquid-Liquid Extraction / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D020404:Glycerophospholipids