title: Tandem repeats in a new toxin gene from Bacillus thuringiensis and in other cry11-like genes.
abstract: A new gene, cry11Bb2 from a field isolate of Bacillus thuringiensis, was cloned for expression in Escherichia coli. The encoded protein, with a deduced molecular mass of 89.5 kDa, exhibits 97 and 79% identities with the overlap regions of Cry11Bb1 from B. thuringiensis ssp. medellin and Cry11Ba1 from ssp. jegathesan, respectively. It is however longer than Cry11Bb1 by 42 amino acids in its carboxy-terminus, of which 32 comprise 2 tandem repeats additional to the 5 existing in the latter polypeptide. Possible roles for this recurrent motif among Cry toxins and their accessory proteins, and for their encoding genes are proposed.
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
authors.forename: Olga
authors.initials: O
authors.lastname: Melnikov
authors.forename: Nadine
authors.initials: N
authors.lastname: Baranes
authors.forename: Monica
authors.initials: M
authors.lastname: Einav
authors.forename: Eitan
authors.initials: E
authors.lastname: Ben-Dov
authors.forename: Robert
authors.initials: R
authors.lastname: Manasherob
authors.forename: Mark
authors.initials: M
authors.lastname: Itsko
authors.forename: Arieh
authors.initials: A
authors.lastname: Zaritsky
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D020080:Tandem Repeat Sequences / Q000235:genetics*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis