title: [Application of multiprobe fluorescence in situ hybridization panel in the diagnosis of acute myeloid leukemia].
abstract: OBJECTIVE
  To assess the value of multiprobe fluorescence in situ hybridization (FISH) panel in the diagnosis of acute myeloid leukemia (AML).
  
  
  METHODS
  The multiprobe AML/MDS panel comprising 8 different FISH probes for AML1/ETO transfusion gene, PML-RARα transfusion gene, CBFβ/MYH11 transfusion gene, MLL breakapart, P53 deletion, Del(5q), -7/Del(7q), and Del(20q) was tested in 40 cases of AML, and the results were compared with those by conventional cytogenetic G-banding (CCG) test.
  
  
  RESULTS
  With multiprobe FISH panel, 22 of the 40 AML cases were found to carry 7 types of cytogenetic abnormalities, namely AML1/ETO transfusion gene, PML-RARα transfusion gene, MLL breakapart, P53 deletion, Del(5q), -7/Del(7q) and trisomy 8. The positive ratio of the multiprobe FISH was 57.5%. CCG only identified 8 cases with the corresponding cytogenetic abnormalities and 3 cases with other cytogenetic abnormalities, and the positive ratio was only 27.50%.
  
  
  CONCLUSIONS
  Mutiprobe FISH panel is more rapid, accurate and effective than CCG in the diagnosis of AML.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lulusuc@163.com
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Xu
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: DU
authors.forename: Lan-Lin
authors.initials: LL
authors.lastname: Song
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Na
authors.initials: N
authors.lastname: Xu
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Zhang
authors.forename: Bin-Tao
authors.initials: BT
authors.lastname: Huang
authors.forename: Xu-Jing
authors.initials: XJ
authors.lastname: Luo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015342:DNA Probes; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D015342:DNA Probes