title: Posterolateral spinal fusion with nano-hydroxyapatite-collagen/PLA composite and autologous adipose-derived mesenchymal stem cells in a rabbit model.
abstract: Spinal fusion is routinely performed to treat low back pain caused by degeneration of intervertebral discs. An autologous bone graft derived from the iliac crest is the standard procedure used for spinal fusion. However, several shortcomings, including pseudarthrosis, pain and the need for blood transfusion are known to be associated with the procedure. Our study analysed the effectiveness of a new mineralized collagen matrix, nano-hydroxyapatite-collagen-polylactic acid (nHAC-PLA), combined with autologous adipose-derived mesenchymal stem cells (ADMSCs) as a graft material for posterolateral spinal fusion in a rabbit model. Forty rabbits were randomly divided into four groups: autologous iliac crest bone group (ACB), nHAC-PLA composite group (nHAC-PLA), autologous iliac crest bone mixed with nHAC-PLA composite group (ACB + nHAC-PLA), and nHAC-PLA composite combined with ADMSCs (ADMSCs + nHAC-PLA). The viability and the proliferation of the ADMSCs seeded on the scaffolds were evaluated by live/dead kit and MTT assay in vitro, respectively. Lumbar posterolateral fusions were assessed by manual palpation, radiographical and histological procedures, mechanical strength and scanning electronic microscopy (SEM) in 10 weeks of observation. The results showed that the rate of fusion was significantly higher in the ACB and ADMSCs + nHAC-PLA groups than that in the nHAC-PLA and ACB + nHAC-PLA groups. It was not significantly higher in the ACB group than in the ADMSCs + nHAC-PLA group. From microstructural analysis of the samples using histological staining methods, there was more new bone-like tissue formation in the ACB and ADMSCs + nHAC-PLA groups than that in the other two groups at the 10th postoperative week. Our study demonstrated the effective impact of nHAC-PLA combined with ADMSCs in rabbit posterolateral spinal fusion.
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Centre, Academy of Military Medical Sciences, 27 Taiping Road, Beijing 100850, People's Republic of China.
authors.forename: Zi-Bin
authors.initials: ZB
authors.lastname: Tang
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Cao
authors.forename: Ning
authors.initials: N
authors.lastname: Wen
authors.forename: Hai-Bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Zhong-Wen
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Cui-Mi
authors.initials: CM
authors.lastname: Duan
authors.forename: Fu-Zhai
authors.initials: FZ
authors.lastname: Cui
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D019344:Lactic Acid / Q000494:pharmacology*; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D023421:Models, Animal; D015675:Osteocalcin / Q000378:metabolism; D010173:Palpation; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D019919:Prosthesis Implantation; D011817:Rabbits; D011859:Radiography; D013123:Spinal Fusion / Q000379:methods*; D013131:Spine / Q000000981:diagnostic imaging / Q000187:drug effects / Q000601:surgery; D014182:Transplantation, Autologous
chemical_list: D011091:Polyesters; D011108:Polymers; C509317:nano-hydroxyapatite-collagen; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite