title: C-S targeted biodegradation of dibenzothiophene by Stenotrophomonas sp. NISOC-04.
abstract: Crude oil-contaminated soil samples were gathered across Khuzestan oilfields (National Iranian South Oil Company, NISOC) consequently experienced a screening procedure for isolating C-S targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. Among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a C-S targeted manner in aqueous phases and medium mostly consisting of separately biphasic water-gasoline. The 16S rDNA of the isolate was amplified using eubacterial-specific primers and then sequenced. Based on sequence data analysis, the microorganism, designated NISOC-04, clustered most closely with the members of the genus Stenotrophomonas. Gas chromatography indicated that Stenotrophomonas sp. NISOC-04 utilizes 82% of starting 0.8 mM dibenzothiophene within a 48-h-long exponential growth phase. Growth curve analysis revealed the inability of Stenotrophomonas sp. NISOC-04 to utilize dibenzothiophene (DBT) as the exclusive carbon or carbon/sulfur source. Gibbs' assay showed no 2-hydroxy biphenyl accumulation, but HPLC confirmed the presence of 2-hydroxy biphenyl as the final product of DBT desulfurization. Under sulfur starvation, Stenotrophomonas sp. NISOC-04 produced a huge biomass with untraceable sulfur and utilized atmospheric insignificant sulfur levels.
authors.affiliation: Biotechnology & Biology Research Center, Shahid Chamran University, Ahvaz, Iran. moslempapizadeh@yahoo.com
authors.forename: Moslem
authors.initials: M
authors.lastname: Papizadeh
authors.forename: Mohammad Roayaei
authors.initials: MR
authors.lastname: Ardakani
authors.forename: Hossein
authors.initials: H
authors.lastname: Motamedi
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasouli
authors.forename: Mohammad
authors.initials: M
authors.lastname: Zarei
mesh_terms: D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005742:Gasoline; D010578:Petroleum / Q000378:metabolism*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000378:metabolism*; D020588:Stenotrophomonas / Q000235:genetics / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
chemical_list: D001713:Biphenyl Compounds; D005742:Gasoline; D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D013876:Thiophenes; D013455:Sulfur; D002244:Carbon; C004369:2-phenylphenol; C016366:dibenzothiophene