title: DNA detection with a polymeric nanochannel device.
abstract: We present the development and the electrical characterization of a polymeric nanochannel device. Standard microfabrication coupled to Focused Ion Beam (FIB) nanofabrication is used to fabricate a silicon master, which can be then replicated in a polymeric material by soft lithography. Such an elastomeric nanochannel device is used to study DNA translocation events during electrophoresis experiments. Our results demonstrate that an easy and low cost fabrication technique allows creation of a low noise device for single molecule analysis.
authors.affiliation: Nanomed Labs, Dipartimento di Fisica, Università di Genova, via Dodecaneso 33, 16146 Genova, Italy. paola.fanzio@iit.it
authors.forename: Paola
authors.initials: P
authors.lastname: Fanzio
authors.forename: Valentina
authors.initials: V
authors.lastname: Mussi
authors.forename: Chiara
authors.initials: C
authors.lastname: Manneschi
authors.forename: Elena
authors.initials: E
authors.lastname: Angeli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Firpo
authors.forename: Luca
authors.initials: L
authors.lastname: Repetto
authors.forename: Ugo
authors.initials: U
authors.lastname: Valbusa
mesh_terms: D004247:DNA / Q000032:analysis*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D012825:Silicon / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon; D004247:DNA; D012825:Silicon