title: Analysis of the effector activity of Ara h 2 and Ara h 6 by selective depletion from a crude peanut extract.
abstract: It is important to know the contribution of specific allergens to a complex allergenic extract and to have a dependable method to assess the effector activity of an extract specifically depleted of that allergen. We have previously shown that removal of the major peanut allergen, Ara h 2, from a crude peanut extract (CPE) minimally altered the effector activity of the extract. Here we describe in detail the methodology used to generate specific rabbit anti-peptide antibodies to remove a related peanut allergen, Ara h 6, from CPE and describe an improvement in the RBL SX-38 cell assay used to assess the effector activity of treated extracts. Our results show that although Ara h 2 and Ara h 6 can be selectively removed from a CPE, removal of each alone from a CPE had no significant effect on the effector activity. However, removal of Ara h 2 and Ara h 6 together significantly reduced the effector activity of CPE.
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA.
authors.forename: Xueni
authors.initials: X
authors.lastname: Chen
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Daphne
authors.initials: D
authors.lastname: Moutsoglou
authors.forename: Glenn
authors.initials: G
authors.lastname: Ruiz
authors.forename: S-E
authors.initials: SE
authors.lastname: Yen
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Dreskin
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000494:pharmacology*; D000917:Antibody Formation; D052179:Antigens, Plant / Q000276:immunology*; D015549:Basophil Degranulation Test; D045424:Complex Mixtures / Q000276:immunology; D006023:Glycoproteins / Q000172:deficiency / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D021183:Peanut Hypersensitivity / Q000097:blood / Q000276:immunology*
chemical_list: D055516:2S Albumins, Plant; D000906:Antibodies; D052179:Antigens, Plant; C500890:Ara h 2 allergen, Arachis hypogaea; C492737:Ara h 6 allergen, Arachis hypogaea; D045424:Complex Mixtures; D006023:Glycoproteins