title: Easy strategy to increase salt resistance of antimicrobial peptides.
abstract: The efficacies of many antimicrobial peptides are greatly reduced under high salt concentrations, limiting their development as pharmaceutical compounds. Here, we describe an easy strategy to increase salt resistance of antimicrobial peptides by replacing tryptophan or histidine residues with the bulky amino acids β-naphthylalanine and β-(4,4'-biphenyl)alanine. The activities of the salt-sensitive peptide P-113 were diminished at high salt concentrations, whereas the activities of its β-naphthylalanine and β-(4,4'-biphenyl)alanine-substituted variant were less affected.
authors.affiliation: Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan.
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Yu
authors.forename: Chih-Hsiung
authors.initials: CH
authors.lastname: Tu
authors.forename: Bak-Sau
authors.initials: BS
authors.lastname: Yip
authors.forename: Heng-Li
authors.initials: HL
authors.lastname: Chen
authors.forename: Hsi-Tsung
authors.initials: HT
authors.lastname: Cheng
authors.forename: Kuo-Chun
authors.initials: KC
authors.lastname: Huang
authors.forename: Hsiu-Jung
authors.initials: HJ
authors.lastname: Lo
authors.forename: Jya-Wei
authors.initials: JW
authors.lastname: Cheng
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000737:chemistry; D002175:Candida / Q000187:drug effects*; D054808:Histatins / Q000737:chemistry* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D054712:Salinity; D055049:Salt Tolerance; D012965:Sodium Chloride; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D054808:Histatins; C570444:beta-(4,4'-biphenyl)alanine; D015091:beta-Alanine; D012965:Sodium Chloride; C063914:beta-naphthylalanine; D000409:Alanine