title: Pyridoxamine protects protein backbone from oxidative fragmentation.
abstract: Oxidative damage to proteins is one of the major pathogenic mechanisms in many chronic diseases. Therefore, inhibition of this oxidative damage can be an important part of therapeutic strategies. Pyridoxamine (PM), a prospective drug for treatment of diabetic nephropathy, has been previously shown to inhibit several oxidative and glycoxidative pathways, thus protecting amino acid side chains of the proteins from oxidative damage. Here, we demonstrated that PM can also protect protein backbone from fragmentation induced via different oxidative mechanisms including autoxidation of glucose. This protection was due to hydroxyl radical scavenging by PM and may contribute to PM therapeutic effects shown in clinical trials.
authors.affiliation: Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
authors.forename: Sergei
authors.initials: S
authors.lastname: Chetyrkin
authors.forename: Missy
authors.initials: M
authors.lastname: Mathis
authors.forename: W
authors.initials: W
authors.lastname: Hayes McDonald
authors.forename: Xavier
authors.initials: X
authors.lastname: Shackelford
authors.forename: Billy
authors.initials: B
authors.lastname: Hudson
authors.forename: Paul
authors.initials: P
authors.lastname: Voziyan
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry*; D011733:Pyridoxamine / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000975:Antioxidants; D011506:Proteins; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D011733:Pyridoxamine; D012260:Ribonucleases; D009113:Muramidase; D005947:Glucose