title: [Simultaneous determination of four active compounds in tiangou capsules by HPLC].
abstract: OBJECTIVE
  To establish a method for quantitative analysis of gastrodin, baicalin, paeonolum and pinoresinol diglucoside in Tiangou capsules by HPLC.
  
  
  METHODS
  The separation was performed on SinoChrom ODS-BP column (4.6 mm x 150 mm, 5 microm). The mobile phase consisted of acetonitrile and 0.2% phosphoric acid aqueous solution with gradient elution program. The flow rate was 1.0 ml x min(-1) and the column temperature was 30 degrees C. UV detection wavelength was set at 230 nm.
  
  
  RESULTS
  The linear ranges of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 0.1168-1.1680, 0.1142-1.1420, 0.0512-0.5120, 0.0556-0.5560 microg, respectively. The average recoveries of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 100.3% (RSD 0.53%), 99.96% (RSD 1.15%), 99.90% (RSD 1.17%), 99.97% (RSD 1.62%), respectively.
  
  
  CONCLUSIONS
  The method is accurate, simple, feasible and reliable, and is available for the quality control of Tiangou capsules.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250355, China. wangxin470920844@126.com
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Dai
mesh_terms: D001592:Benzyl Alcohols / Q000737:chemistry*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D017705:Lignans / Q000737:chemistry*; D016014:Linear Models; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D001592:Benzyl Alcohols; D002214:Capsules; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005663:Furans; D005960:Glucosides; D017705:Lignans; D009930:Organic Chemicals; C560376:Tiangou