title: A study of elevated interleukin-8 (CXCL8) and detection of leukocyte migration inhibitory activity in patients allergic to beta-lactam antibiotics.
abstract: BACKGROUND
  The leukocyte migration test (LMT) is effective in identifying the causative drug in drug allergies. Both leukocyte migration activating activity (LMAA) and leukocyte migration inhibitory activity (LMIA) are involved in the development of drug allergies. However, no cytokines associated with LMIA have been identified to date. Because CXCL8 played an important role in neutrophil infiltration and activation, we performed the LMT and measured CXCL8 levels in patients with hypersensitivity to beta-lactam antibiotics (beta-lactams) and antipyretic analgesics (APAs) and investigated the pathogenic mechanism of hypersensitivity to these drugs.
  
  
  METHODS
  The LMT was performed according to an improved version of the agarose plate method and CXCL8 levels in the reacted solution that had been stored as described were measured using a solid-phase sandwich enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Migration index (MI) values for the LMT were 77.7 ± 11.7 for patients with hypersensitivity to beta-lactams and 83.6 ± 1.9 for those with hypersensitivity to APAs. The CXCL8 concentrations were significantly higher in patients after beta-lactams administration (175.9 ± 71.2 ng/mL) than those without beta-lactams administration (48.3 ± 34.9 ng/mL). The CXCL8 concentrations were significantly lower in patients after APAs administration (41.7 ± 24.3 ng/mL) than those without APAs administration (63.1 ± 30.2 ng/mL).
  
  
  CONCLUSIONS
  Increased CXCL8 levels produced by beta-lactams administration were accompanied by LMIA. CXCL8 may be involved in LMIA and play a role in beta-lactam allergies. In contrast, the LMIA detected in patients with allergies to APAs may be a cytokine or chemokine other than CXCL8.
authors.affiliation: Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan. abe@nupals.ac.jp
authors.forename: Manabu
authors.initials: M
authors.lastname: Abe
authors.forename: Motohiro
authors.initials: M
authors.lastname: Yagi
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Wakasugi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hattori
authors.forename: Katsuji
authors.initials: K
authors.lastname: Uno
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D054441:Cell Migration Assays, Leukocyte; D002464:Cell Migration Inhibition / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004342:Drug Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult; D047090:beta-Lactams / Q000009:adverse effects* / Q000276:immunology
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D047090:beta-Lactams