title: [Correlation of mRNA and protein expressions of Runx3 gene in papillary thyroid carcinoma].
abstract: OBJECTIVE
  To explore the mRNA and protein expressions of Runx3 gene in papillary thyroid carcinoma (PTC).
  
  
  METHODS
  Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the Runx3 mRNA and protein levels in 67 human PTC specimens and their matched adjacent non-cancerous epithelium (NCE).
  
  
  RESULTS
  The relative expression value of Runx3 mRNA was 0.31 ± 0.07 in PTC versus 0.92 ± 0.08 in NCE (t = 38.251, P < 0.05). In PTC, it was correlated with the tumor pathological grade and lymph node metastasis (χ(2) = 5.511, 6.492, P < 0.05). The integral optical density value of Runx3 protein confirmed was 1012 ± 221 in PTC versus 1993 ± 199 in NCE (t = 18.413, P < 0.05). In PTC, it was correlated with the tumor TNM stage, pathological grade and lymph node metastasis (χ(2) = 5.550, 9.678, 5.070, P < 0.05). Furthermore there was a distinct correlation between the mRNA and protein expressions of Runx3 gene (χ(2) = 42.699, P < 0.05).
  
  
  CONCLUSIONS
  The mRNA and protein expressions of Runx3 gene in PTC were lower than those in NCE. A lower expression of Runx3 gene may play an important role in the carcinogenesis and progression of PTC.
authors.affiliation: Department of Thyroid Surgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. detaoyin@zzu.edu.cn
authors.forename: De-tao
authors.initials: DT
authors.lastname: Yin
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Cao
authors.forename: Yong-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Hong-qiang
authors.initials: HQ
authors.lastname: Li
authors.forename: Yu-bing
authors.initials: YB
authors.lastname: Zhou
authors.forename: Li-yun
authors.initials: LY
authors.lastname: Zheng
authors.forename: Jin-hua
authors.initials: JH
authors.lastname: Jiang
authors.forename: Qing-duan
authors.initials: QD
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002277:Carcinoma; D002291:Carcinoma, Papillary; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D012333:RNA, Messenger; C493729:Runx3 protein, human