title: Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency.
abstract: There is currently an unmet need for the supply of autologous, patient-specific stem cells for regenerative therapies in the clinic. Mesenchymal stem cell differentiation can be driven by the material/cell interface suggesting a unique strategy to manipulate stem cells in the absence of complex soluble chemistries or cellular reprogramming. However, so far the derivation and identification of surfaces that allow retention of multipotency of this key regenerative cell type have remained elusive. Adult stem cells spontaneously differentiate in culture, resulting in a rapid diminution of the multipotent cell population and their regenerative capacity. Here we identify a nanostructured surface that retains stem-cell phenotype and maintains stem-cell growth over eight weeks. Furthermore, the study implicates a role for small RNAs in repressing key cell signalling and metabolomic pathways, demonstrating the potential of surfaces as non-invasive tools with which to address the stem cell niche.
authors.affiliation: Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow, G12 8QQ, Scotland, UK.
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: McMurray
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Gadegaard
authors.forename: P Monica
authors.initials: PM
authors.lastname: Tsimbouri
authors.forename: Karl V
authors.initials: KV
authors.lastname: Burgess
authors.forename: Laura E
authors.initials: LE
authors.lastname: McNamara
authors.forename: Rahul
authors.initials: R
authors.lastname: Tare
authors.forename: Kate
authors.initials: K
authors.lastname: Murawski
authors.forename: Emmajayne
authors.initials: E
authors.lastname: Kingham
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
mesh_terms: D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D013499:Surface Properties