title: Dental implants osteogenic properties evaluated by cDNA microarrays.
abstract: OBJECTIVE
  This study aims at applying cDNA microarray analysis in vitro for establishing and comparing the osteogenic properties of dental implants with different surface characteristics.
  
  
  METHODS
  Saos-2 osteoblasts were cultured in bottom-cone tubes in presence of 5 different dental implants with various surface characteristics. Cells adherent to dental implants were detached and RNA purified. The expression of 18,401 genes was tested by cDNA microarray.
  
  
  RESULTS
  The number and viability of cells adherent to different dental implants varied but without any significant statistical difference. Conversely, gene expression was revealed to be a more sensitive biomarker being remarkably different in cells adherent to different implants. The 5 dental implants significantly modulated the expression of 14 osteogenic activities mainly including bone morphogenetic proteins, osteomodulin, and osteoprotegerin.
  
  
  CONCLUSIONS
  Despite no significant differences having been found in in vitro cell number and viability, cells adherent to 5 differently surfaced implants showed different gene expression profiles. Thus, to evaluate osteogenesis as related to dental implants, it is important to analyze not only the number of adherent cells but also the activation of genes encoding for osteogenic activities.
authors.affiliation: Department of Odontostomatologic Sciences, Faculty of Medicine, University of Genoa, Genoa, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Baldi
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Longobardi
authors.forename: Cristina
authors.initials: C
authors.lastname: Cartiglia
authors.forename: Sebastiano
authors.initials: S
authors.lastname: La Maestra
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pulliero
authors.forename: Paolo
authors.initials: P
authors.lastname: Bonica
authors.forename: Rosana Tindara
authors.initials: RT
authors.lastname: Micale
authors.forename: Maria
authors.initials: M
authors.lastname: Menini
authors.forename: Paolo
authors.initials: P
authors.lastname: Pera
authors.forename: Alberto
authors.initials: A
authors.lastname: Izzotti
mesh_terms: D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics; D002448:Cell Adhesion; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D018076:DNA, Complementary / Q000235:genetics; D015921:Dental Implants; D017267:Dental Prosthesis Design; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D053244:Osteoprotegerin / Q000096:biosynthesis / Q000235:genetics; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D013499:Surface Properties
chemical_list: D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D053244:Osteoprotegerin; D011509:Proteoglycans; C113360:osteoadherin