title: [Influence of different processing methods on Angelica sinensis polysaccharides from same origin].
abstract: OBJECTIVE
  To study the influences of different processing methods on the content of Angelica sinensis polysaccharides (APS) from the same origin.
  
  
  METHODS
  The contents of neutral polysaccharides and acidic polysaccharides in various samples of A. sinensis were determined by phenol-sulfuric acid and carbazole-sulfuric acid method, respectively. The proliferation ability of lymphocyte was detected by MTT method after the cells were cultured with different concentrations of APS from two samples processed by different methods.
  
  
  RESULTS
  The different processing methods had different effects on the contents of polysaccharide. The maximum content of APS (26.03%) was found in the sample processed by microwave drying medium-fired, but the minimum content of APS (2.25%) was found in the sample processed by vacuum drying at 50 TC. Furthermore, the APS (high concentration group, P < 0.01) processed by microwave drying medium-fired could both accelerate proliferation of spleen lymphocytes directly and increase proliferation of T cells of mice induced by Con A. However, the APS processed by far-infrared drying did not show conspicuous immune enhancement activity.
  
  
  CONCLUSIONS
  Different processing methods have different effects on the contents of APS and the proliferation ability of lymphocytes.
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China. ruoxin0371@163.com
authors.forename: Jieli
authors.initials: J
authors.lastname: Lv
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
authors.forename: Hui
authors.initials: H
authors.lastname: Yan
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Bingsheng
authors.initials: B
authors.lastname: Song
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004339:Drug Compounding / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides