title: Summarizing probe intensities of affymetrix GeneChip 3' expression arrays taking into account day-to-day variability.
abstract: Microarray experiments are affected by several sources of variability. The paper demonstrates the major role of the day-to-day variability, it underlines the importance of a randomized block design when processing replicates over several days to avoid systematic biases and it proposes a simple algorithm that minimizes the day dependence.
authors.affiliation: Università degli Studi di Pavia, Pavia. paolo.magni@unipv.it
authors.forename: Paolo
authors.initials: P
authors.lastname: Magni
authors.forename: Angela
authors.initials: A
authors.lastname: Simeone
authors.forename: Sandra
authors.initials: S
authors.lastname: Healy
authors.forename: Antonella
authors.initials: A
authors.lastname: Isacchi
authors.forename: Roberta
authors.initials: R
authors.lastname: Bosotti
mesh_terms: D000465:Algorithms; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D019295:Computational Biology / Q000592:standards*; D020869:Gene Expression Profiling / Q000592:standards*; D006801:Humans; D015233:Models, Statistical; D015335:Molecular Probes; D020411:Oligonucleotide Array Sequence Analysis / Q000592:standards*; D015203:Reproducibility of Results
chemical_list: D015335:Molecular Probes