title: [Anti GVHD effect of the induction of immune tolerance in rats embryo].
abstract: OBJECTIVE
  To investigate whether the fetal immune tolerance induction could replace the HLA typing for hematopoietic stem cell transplantation.
  
  
  METHODS
  Immune tolerance of SD rats was induced by injecting host Wistar rats peripheral blood mononuclear cells into yolk sac of the embryo, afterward the mature male offsprings were used as donor. The host female recipients received lethal dose irradiation and bone marrow transplantation(BMT). The Wistar rats transplanted with bone marrow from donor and unrelated SD rats as well as the rats which received radiation alone were used as control. The survival, histopathologically GVHD, the mental status, food and water intake, coat characteristics, activities were observed. Forty days after BMT, autologous and allogenous skin transplantation between donor and recipient rats was performed to observe the engraftment of solid organ.
  
  
  RESULTS
  The survival of the rats received bone marrow grafts from the immune tolerant donor was significantly longer than that of control groups (30 day survival rates were 86.7%, 6.7%, 0%, and 0% respectively), and there was no histopathologically GVHD observed, while in the sham group, the manifestations of GVHD was clearly visible. The skin engraftment rate between the host and the immune tolerant donor was significantly higher than that among non-related rats (84.6% and 0% respectively).
  
  
  CONCLUSIONS
  The induction of immune tolerance in embryo can overcome the HLA barrier and provide a good donor for hematopoietic stem cell and solid organ transplantation.
authors.affiliation: Rheumatology Department, Hematology Department, First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yao
authors.forename: Rui-Lian
authors.initials: RL
authors.lastname: Zhou
authors.forename: Ye-Hui
authors.initials: YH
authors.lastname: Tan
authors.forename: Chang
authors.initials: C
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Jiang
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000276:immunology; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology* / Q000517:prevention & control; D018380:Hematopoietic Stem Cell Transplantation; D006650:Histocompatibility Testing; D007165:Immunosuppression Therapy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D018183:Transplantation Chimera