title: Production of 2,3-butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens.
abstract: In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3-butanediol (2,3-BD) designated as B10-127 was isolated in our lab. The strain B10-127 produced 2,3-BD effectively under the condition of 20% glucose (quality concentration), showed a high-glucose tolerance. The effects of initial glucose concentration, temperature, pH and agitation on 2,3-BD production were investigated in this work and the proper parameters were identified. Accordingly, the fed-batch culture of B10-127 in larger scales (5 l) showed a remarkable 2,3-BD producing potency. The maximum 2,3-BD concentration reached 92.3 g/l at 96 h with a 2,3-BD productivity of 0.96 g/l h. To our knowledge, the results were new records on 2,3-BD fermentation by Bacillus, which shown an excellent candidate for the microbial fermentation of 2,3-BD on an industrial scale.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu Province 214122, PR China.
authors.forename: Taowei
authors.initials: T
authors.lastname: Yang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Rao
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Haifeng
authors.initials: H
authors.lastname: Xia
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
authors.forename: Shangtian
authors.initials: S
authors.lastname: Yang
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D005947:Glucose