title: Chondrogenesis of mesenchymal stem cells and dedifferentiated chondrocytes by transfection with SOX Trio genes.
abstract: In this study, bone marrow-derived mesenchymal stem cells (MSCs), adipose-derived mesenchymal stem cells (ASCs) and dedifferentiated chondrocytes were transfected with SOX5, 6, and 9 genes (SOX Trio) and grown under pellet culture conditions (encapsulated in a fibrin hydrogel) to evaluate the chondrogenic potential in vitro and in vivo. RT-PCR, real-time quantitative PCR (qPCR), histology, and immunohistochemical assays were performed to determine the chondrogenic potential of the stem cells and dedifferentiated chondrocytes. Chondrogenic genes and proteins were more highly expressed in SOX Trio-expressing cells than in untransfected cells. In addition, not only specific genes and proteins, but cartilage-forming tissues were observed in nude mice transplanted with fibrin hydrogel encapsulated SOX Trio-expressing MSCs, ASCs, and dedifferentiated chondrocytes. Both in vitro and in vivo analyses revealed that fibrin hydrogel encapsulated cultured or transplanted cells transfected with the SOX Trio successfully differentiated into mature chondrocytes and could be used for the reconstruction of hyaline articular cartilage.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, 606-16 Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Republic of Korea.
authors.forename: Han Na
authors.initials: HN
authors.lastname: Yang
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Dae Gyun
authors.initials: DG
authors.lastname: Woo
authors.forename: Su Yeon
authors.initials: SY
authors.lastname: Jeon
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Do
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lim
authors.forename: Seung Who
authors.initials: SW
authors.lastname: Kim
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D054337:Cell Dedifferentiation; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D020219:Chondrogenesis; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D055758:SOX9 Transcription Factor / Q000235:genetics*; D055755:SOXD Transcription Factors / Q000235:genetics*; D014162:Transfection
chemical_list: D055758:SOX9 Transcription Factor; D055755:SOXD Transcription Factors