title: [Determination of diosgenin and ruscogenin in Radix Ophiopogonis by nonaqueous capillary electrophoresis].
abstract: Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.
authors.affiliation: Chemical Department of Mianyang Normal University, Mianyang 621000, China. hbm790117@yahoo.com.cn
authors.forename: Bao-mei
authors.initials: BM
authors.lastname: Huang
authors.forename: Cheng-wei
authors.initials: CW
authors.lastname: Yao
authors.forename: Qing-quan
authors.initials: QQ
authors.lastname: Bian
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Jin-yuan
authors.initials: JY
authors.lastname: Mo
mesh_terms: D004144:Diosgenin / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D031441:Ophiopogon / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D013150:Spirostans / Q000032:analysis*
chemical_list: D013150:Spirostans; C012541:ruscogenin; D004144:Diosgenin