title: Distribution and pharmacokinetics of alkylphenolic compounds in primary mouse hepatocyte cultures.
abstract: In order to allow an interpretation of in vitro results on the estrogenic potency of 4-nonylphenol (NP) and 4-octylphenol (OP), their distribution and pharmacokinetics were investigated in primary mouse hepatocyte cultures. 10(-5) M NP and 10(-6)M OP were added to the hepatocyte cultures and incubated up to 48 h. The time course of parent NP and OP concentrations was followed with GC-MS both in the medium and in the cell fraction. Both NP and OP concentrations decreased rapidly in the cell cultures, indicating that the alkylphenols underwent a rapid metabolism. The concentrations in the cell fractions were 100 ng NP and 2-6 ng OP/mg cell protein after 2 h, declining to about 20 ng NP and 1-4 ng OP/mg cell protein, respectively, after 48 h.
authors.affiliation: Institute of Toxicology, Swiss Federal Institute of Technology and University of Zurich, Schorenstrasse 16, CH-8603 Schwerzenbach, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Müller
authors.forename: P
authors.initials: P
authors.lastname: Schmid
authors.forename: C
authors.initials: C
authors.lastname: Schlatter