title: [Transplantation of bone marrow-derived endothelial progenitor cells preconditioned with ex vivo 17β-estradiol enhances healing efficacy after acute myocardial infarction].
abstract: OBJECTIVE
  To investigate the therapeutic efficacy of intravenous implanted bone marrow-derived endothelial progenitor cells (BM-EPC) preconditioned with 17β-estradiol in ovariectomized mice model of acute myocardial infarction (AMI).
  
  
  METHODS
  BM-EPC were cultured and identified from ovariectomized BALB/C mice tibia and femur. The ovariectomized BALB/C mice models of acute myocardial infarction (AMI) were established, and randomly divided into 17β-estradiol + BM-EPC group (n = 6), BM-EPC group (n = 6) and control group (n = 6). Three days after AMI, BM-EPC pretreated with or without 17β-estradiol was infused via tail vein. The equal volume of saline was infused in control group. Twenty-five days after infusion, left ventricular (LV) function and dimensions, capillary density and ratio of fibrosis area to LV area were measured.
  
  
  RESULTS
  LV function and dimensions, capillary density and LV fibrosis were significantly improved in 17β-estradiol + BM-EPC group than in control group [(LVDs: (3.09 ± 0.05) mm vs. (3.27 ± 0.10)mm, P < 0.05; LVDd: (4.18 ± 0.07) mm vs. (4.31 ± 0.05) mm, P < 0.05; FS: (33.0 ± 3.8)% vs. (26.0 ± 3.2)%, P < 0.05; capillary density: (1428 ± 214)/mm² vs. (1070 ± 168)/mm², P < 0.05; ratio of fibrosis: (38.8 ± 4.9)% vs. (49.0 ± 4.6)%, P < 0.05]. However, Above mentioned parameters were similar between BM-EPC group and control group (P > 0.05).
  
  
  CONCLUSIONS
  BM-EPC preconditioned with 17β-estradiol can enhance capillary density, decrease LV fibrosis and improve cardiac function in this mice model of AMI.
authors.affiliation: Department of Cardiac Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Ye
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology*; D004958:Estradiol; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009203:Myocardial Infarction / Q000601:surgery*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D019172:Transplantation Conditioning / Q000379:methods*
chemical_list: D004958:Estradiol