title: CD34 expression in human hair follicles and tricholemmoma: a comprehensive study.
abstract: BACKGROUND
  There has recently been controversy regarding whether clone My10 is superior to clone QBEND-10 for labeling cells of tricholemmal lineage. Moreover, there have been no previous reports on the CD34 expression in human vellus hair follicles.
  
  
  METHODS
  We performed a comprehensive study of the CD34 expression in human terminal and vellus hair follicles and in 10 tricholemmomas using both the QBEND-10 and the My10 clones. We also performed two different procedures of immunostaining, which included the using of the standard avidin-biotin-peroxidase (ABC) complex system and the Envision system.
  
  
  RESULTS
  The most sensitive marker of CD34 for normal human hair follicles and tricholemmomas is QBEND-10 using the ABC system. The degree and strength of the CD34 positive staining mainly depended on the method being used (whether it was the ABC system or the Envision system) rather than the clone. CD34 staining was rarely (20-30%) seen in the anagen and catagen vellus hair follicles, and could only be seen by the QBEND-10 clone using the ABC system. CD34 expression in the tricholemmomas represented either a diffuse or peripheral pattern.
  
  
  CONCLUSIONS
  CD34 may not be a tricholemmal lineage-specific antigen, but may be related to certain functions of the cells.
authors.affiliation: Division of Dermatology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan. misago@post.saga-med.ac.jp
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Misago
authors.forename: Shuji
authors.initials: S
authors.lastname: Toda
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Narisawa
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34 / Q000276:immunology / Q000378:metabolism*; D006201:Hair Diseases / Q000378:metabolism / Q000473:pathology*; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008562:Membrane Glycoproteins / Q000276:immunology; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D008562:Membrane Glycoproteins; C050120:My-10 glycoprotein