title: Laser-capture microdissection for Factor VIII-expressing endothelial cells in cancer tissues.
abstract: The immunostaining based Laser-capture microdissection (LCM) method called immune-LCM allows us to quantify the mRNA. Immune-LCM has recently been introduced to enhance identification of cells carrying a particular protein from frozen tissue samples. We have recently performed the immune-LCM of formaldehyde-fixated, paraffin-embedded tissues immunostained with a monoclonal antibody Factor VIII. This method could be useful for quantitative gene expression analysis in blood vessels from tumors of patients that have been treated with antiangiogenic drugs, allowing for validation of the effect of drug on the expected targets. Such capability might be exceedingly useful for the evaluation of the bioactivity of new drugs. This method is also useful to compare gene expression patterns in tumor cells versus endothelial cells during tumor progression or tumor angiogenesis.
authors.affiliation: Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Niigata, Japan. tomoendo@dent.niigata-u.ac.jp
authors.forename: Tomoatsu
authors.initials: T
authors.lastname: Kaneko
authors.forename: Takashi
authors.initials: T
authors.lastname: Okiji
authors.forename: Reika
authors.initials: R
authors.lastname: Kaneko
authors.forename: Hideaki
authors.initials: H
authors.lastname: Suda
authors.forename: Jacques E
authors.initials: JE
authors.lastname: Nör
mesh_terms: D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D005169:Factor VIII / Q000378:metabolism*; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy / Q000379:methods; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016612:Paraffin Embedding / Q000379:methods; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012034:Refrigeration; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D012313:RNA; D005169:Factor VIII; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases