title: Phosphatidylinositol phospholipase C mediates carbon sensing and vegetative nuclear duplication rates in Aspergillus nidulans.
abstract: In this work, we disrupted one of three putative phosphatidylinositol phospholipase C genes of Aspergillus nidulans and studied its effect on carbon source sensing linked to vegetative mitotic nuclear division. We showed that glucose does not affect nuclear division rates during early vegetative conidial germination (6-7 h) in either the wild type or the plcA-deficient mutant. Only after 8 h of cultivation on glucose did the mutant strain present some decrease in nuclear duplication. However, decreased nuclear division rates were observed in the wild type when cultivated in media amended with polypectate, whereas our plcA-deficient mutant did not show slow nuclear duplication rates when grown on this carbon source, even though it requires induction and secretion of multiple pectinolytic enzymes to be metabolized. Thus, plcA appears to be directly linked to high-molecular-weight carbon source sensing.
authors.affiliation: Laboratório de Enzimologia Industrial, Universidade de São Paulo, Ribeirão Preto-SP, Brazil. apvanzela@yahoo.com.br
authors.forename: Ana Paula de Figueiredo Conte
authors.initials: AP
authors.lastname: Vanzela
authors.forename: Suraia
authors.initials: S
authors.lastname: Said
authors.forename: Rolf Alexander
authors.initials: RA
authors.lastname: Prade
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D048750:Cell Nucleus Division; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D002244:Carbon; D054801:Phosphoinositide Phospholipase C; D005947:Glucose