title: Effect of trehalose coating on basic fibroblast growth factor release from tailor-made bone implants.
abstract: Artificial bone implants are often incorporated with osteoinductive factors to facilitate early bone regeneration. Calcium phosphate, the main component in artificial bone implants, strongly binds these factors, and in a few cases, the incorporated proteins are not released from the implant under conditions of physiological pH, thereby leading to reduction in their osteoinductivity. In this study, we coated tailor-made bone implants with trehalose to facilitate the release of basic fibroblast growth factor (bFGF). In an in vitro study, mouse osteoblastic cells were separately cultured for 48 hr in a medium with a untreated implant (T-), trehalose-coated implant (T+), bFGF-incorporated implant (FT-), and bFGF-incorporated implant with trehalose coating (FT+). In the FT+ group, cell viability was significantly higher than that in the other groups (P<0.05). Scanning electron microscopy (SEM) and X-ray diffraction (XRD) revealed that trehalose effectively covered the surface of the artificial bone implant without affecting the crystallinity or the mechanical strength of the artificial bone implant. These results suggest that coating artificial bone implants with trehalose could limit the binding of bFGF to calcium phosphate.
authors.affiliation: Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. tjdwls1101@hotmail.com
authors.forename: Sungjin
authors.initials: S
authors.lastname: Choi
authors.forename: Jongil
authors.initials: J
authors.lastname: Lee
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Igawa
authors.forename: Shigeki
authors.initials: S
authors.lastname: Suzuki
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nishimura
authors.forename: Ung-il
authors.initials: UI
authors.lastname: Chung
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones; D002130:Calcium Phosphates / Q000737:chemistry; D049109:Cell Proliferation; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D014199:Trehalose / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D016222:Fibroblast Growth Factor 2; C020243:calcium phosphate; D014199:Trehalose