title: Soil matrix tracer contamination and canopy recycling did not impair ¹³CO₂ plant-soil pulse labelling experiments.
abstract: When conducting (13)CO(2) plant-soil pulse labelling experiments, tracer material might cause unwanted side effects which potentially affect δ(13)C measurements of soil respiration (δ(13)C(SR)) and the subsequent data interpretation. First, when the soil matrix is not isolated from the atmosphere, contamination of the soil matrix with tracer material occurs leading to a physical back-diffusion from soil pores. Second, when using canopy chambers continuously, (13)CO(2) is permanently re-introduced into the atmosphere due to leaf respiration which then aids re-assimilation of tracer material by the canopy. Accordingly, two climate chamber experiments on European beech saplings (Fagus sylvatica L.) were conducted to evaluate the influence of soil matrix (13)CO(2) contamination and canopy recycling on soil (13)CO(2) efflux during (13)CO(2) plant-soil pulse labelling experiments. For this purpose, a combined soil/canopy chamber system was developed which separates soil and canopy compartments in order to (a) prevent diffusion of (13)C tracer into the soil chamber during a (13)CO(2) canopy pulse labelling and (b) study stable isotope processes in soil and canopy individually and independently. In combination with laser spectrometry measuring CO(2) isotopologue mixing ratios at a rate of 1 Hz, we were able to measure δ(13)C in canopy and soil at very high temporal resolution. For the soil matrix contamination experiment, (13)CO(2) was applied to bare soil, canopy only or, simultaneously, to soil and canopy of the beech trees. The obtained δ(13)C(SR) fluxes from the different treatments were then compared with respect to label re-appearance, first peak time and magnitude. By determining the δ(13)C(SR) decay of physical (13)CO(2) back-diffusion from bare soils (contamination), it was possible to separate biological and physical components in δ(13)C(SR) of a combined flux of both. A second pulse labelling experiment, with chambers permanently enclosing the canopy, revealed that (13)CO(2) recycling at canopy level had no effect on δ(13)C(SR) dynamics.
authors.affiliation: Institute of Agricultural Sciences, ETH Zurich, Switzerland. mbarthel@ethz.ch
authors.forename: Matthias
authors.initials: M
authors.lastname: Barthel
authors.forename: Patrick
authors.initials: P
authors.lastname: Sturm
authors.forename: Alexander
authors.initials: A
authors.lastname: Knohl
mesh_terms: D001272:Atmosphere / Q000737:chemistry; D002245:Carbon Dioxide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D029964:Fagus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D063245:Plant Development; D018515:Plant Leaves / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000378:metabolism*; D010969:Plastics / Q000737:chemistry*; D059027:Recycling / Q000379:methods*; D012989:Soil Pollutants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D002247:Carbon Isotopes; D010969:Plastics; D012989:Soil Pollutants; D002245:Carbon Dioxide