title: Characterization of gut-derived intraepithelial lymphocyte (IEL) residing in human papillomavirus (HPV)-infected intraepithelial neoplastic lesions.
abstract: OBJECTIVE
  Mucosal T cells are the most likely direct effectors in host anti-human papillomavirus adaptive immunity and regression of cervical intraepithelial neoplasia (CIN) lesions. There are no studies addressing intraepithelial lymphocytes (IELs) in CIN lesions.
  
  
  METHODS
  Cervical lymphocytes were collected using cytobrushes from patients with CIN and analyzed by FACS analysis. Comparisons were made between populations of cervical T cells in CIN regressors and non-regressors.
  
  
  RESULTS
  A median of 74% of cervical lymphocytes were CD3(+) T cells. Populations of integrin αEβ7(+) IEL in CIN lesions varied markedly among patients (6-57%). Approximately half of integrin β7(+) T cells were CD45RA-negative memory T cells. The number of integrin αEβ7(+) cells among cervical T cells was significantly higher in CIN regressors when compared to non-regressors.
  
  
  CONCLUSIONS
  Higher cervical IEL numbers are associated with spontaneous regression of CIN. Accumulation of cervical integrin αEβ7(+) IEL may be necessary for local adaptive effector functions.
authors.affiliation: Faculty of Medicine, Department of Obstetrics and Gynecology, University of Tokyo, Hongo, Bunkyo-ku, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kojima
authors.forename: Kei
authors.initials: K
authors.lastname: Kawana
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujii
authors.forename: Terufumi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Shiho
authors.initials: S
authors.lastname: Miura
authors.forename: Kensuke
authors.initials: K
authors.lastname: Tomio
authors.forename: Ayako
authors.initials: A
authors.lastname: Tomio
authors.forename: Aki
authors.initials: A
authors.lastname: Yamashita
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Adachi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Sato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nagamatsu
authors.forename: Danny J
authors.initials: DJ
authors.lastname: Schust
authors.forename: Shiro
authors.initials: S
authors.lastname: Kozuma
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D002584:Cervix Uteri / Q000276:immunology / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006801:Humans; D018928:Immunity, Mucosal; D016023:Integrins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology*; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications* / Q000276:immunology / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000276:immunology* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000276:immunology* / Q000473:pathology / Q000821:virology
chemical_list: D016023:Integrins; C070709:integrin alphaEbeta7