title: Applicability of thermo-alkali-stable and cellulase-free xylanase from a novel thermo-halo-alkaliphilic Bacillus halodurans in producing xylooligosaccharides.
abstract: An alkaliphilic, moderately thermophilic and halophilic bacterial isolate capable of producing a high titer of extracellular thermo-alkali-stable, cellulase-free endoxylanase was isolated from the paper mill effluents. It was identified as Bacillus halodurans. The purified xylanase was active from pH 7 to 12 and 30 to 100°C with optimal activity at pH 9.0 and 80°C. It had T(1/2) values of 40 and 15 min at 70 and 80°C, respectively. Activity was stimulated by dithiothreitol but strongly inhibited by N-bromosuccinimide. Its action on birchwood xylan and agro-residues liberated xylooligosaccharides of 2-7 degree of polymerization, and thus, the mode of action is similar to endoxylanases of the family 10 glucoside hydrolases.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, New Delhi, India. vikash.udsc@gmail.com
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
mesh_terms: D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D001974:Bromosuccinimide; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004229:Dithiothreitol / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004783:Environmental Microbiology; D004791:Enzyme Inhibitors / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019163:Reducing Agents / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D012336:RNA, Ribosomal, 16S; D019163:Reducing Agents; D043364:Endo-1,4-beta Xylanases; D001974:Bromosuccinimide; D004229:Dithiothreitol