title: Transcriptome analysis of probiotic Lactobacillus casei Zhang during fermentation in soymilk.
abstract: Lactobacillus casei Zhang is a widely recognized probiotic bacterium, which is being commercially used in China. To study the gene expression dynamics of L. casei Zhang during fermentation in soymilk, a whole genome microarray was used to screen for differentially expressed genes when grown to the lag phase, the late logarithmic phase, and the stationary phase. Comparisons of different transcripts next to each other revealed 162 and 63 significantly induced genes in the late logarithmic phase and stationary phase, of which the expression was at least threefold up-regulated and down-regulated, respectively. Approximately 38.4% of the up-regulated genes were associated with amino acid transport and metabolism notably for histidine and lysine biosynthesis, followed by genes/gene clusters involved in carbohydrate transport and metabolism, lipid transport and metabolism, and inorganic ion transport and metabolism. The analysis results suggest a complex stimulatory effect of soymilk-based ecosystem on the L. casei Zhang growth. On the other hand, it provides the very first insight into the molecular mechanism of L. casei strain for how it will adapt to the protein-rich environment.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering, Inner Mongolia Agricultural University, 010018, Huhhot, China.
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Wang
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhong
authors.forename: Ai-Bin
authors.initials: AB
authors.lastname: Wei
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Bao
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tian-Song
authors.initials: TS
authors.lastname: Sun
authors.forename: Andrew
authors.initials: A
authors.lastname: Postnikoff
authors.forename: He
authors.initials: H
authors.lastname: Meng
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Zhang
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002244:Carbon / Q000378:metabolism; D002681:China; D005285:Fermentation; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D017136:Ion Transport / Q000235:genetics; D007780:Lacticaseibacillus casei / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics; D019936:Probiotics / Q000378:metabolism*; D041942:Soy Milk
chemical_list: D000596:Amino Acids; D002244:Carbon