title: Subcellular resolution mapping of endogenous cytokine secretion by nano-plasmonic-resonator sensor array.
abstract: Local extracellular signaling is central for cellular interactions and organizations. We report a novel sensing technique to interrogate extracellular signaling at the subcellular level. We developed an in situ immunoassay based on giant optical enhancement of a tunable nano-plasmonic-resonator array fabricated by nanoimprint lithography. Our nanoplasmonic device significantly increases the signal-to-noise ratio to enable the first time submicrometer resolution quantitative mapping of endogenous cytokine secretion. Our study shows a markedly high local interleukin-2 (IL-2) concentration within the immediate vicinity of the cell which finally validates a decades-old hypothesis on autocrine physiological concentration and spatial range. This general sensing technique can be applied for a broad range of cellular communication studies to improve our understanding of subcellular signaling and function.
authors.affiliation: NSF Nanoscale Science and Engineering Center (NSEC), 3112 Etcheverry Hall, University of California, Berkeley, California 94720, United States.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.forename: Sadao
authors.initials: S
authors.lastname: Ota
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.forename: Jongeun
authors.initials: J
authors.lastname: Ryu
authors.forename: Christopher
authors.initials: C
authors.lastname: Rhodes
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiong
authors.forename: Sheraz
authors.initials: S
authors.lastname: Kalim
authors.forename: Li
authors.initials: L
authors.lastname: Zeng
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007118:Immunoassay; D019169:Jurkat Cells; D036103:Nanotechnology; D013347:Subcellular Fractions / Q000378:metabolism*; D020349:Surface Plasmon Resonance
chemical_list: D016207:Cytokines