title: An IRF-3 homolog that is up-regulated by DNA virus and poly I:C in turbot, Scophthalmus maximus.
abstract: In this study, we described the structure, mRNA tissue distribution and regulation of an IRF-3 gene from turbot, Scophthalmus maximus (SmIRF-3). The gene sequence of SmIRF-3 is 6077 bp long, composed of 11 exons and 10 introns similar to known IRF-3 genes of fish, and encodes a peptide of 466 amino acids. The deduced protein sequence shares the highest identity of 56.0-81.2% with fish IRF-3 and possesses a DNA-binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD) known to be important for the functions of IRF-3 in vertebrates. Phylogenetic analysis grouped SmIRF-3 with other IRF3s of vertebrates. SmIRF-3 transcripts were detectable in limited tissue types of healthy fish, with higher expression observed in head, kidney, spleen and kidney,. The SmIRF-3 was transcriptionally up-regulated by turbot reddish body iridovirus (TRBIV) and polyinosinic:polycytidylic acid (poly I:C) in the head kidney, spleen and gills, with showing a two wave induced expression during a 7-day time course in all cases. The highest inducibility and the likely earliest increase of SmIRF-3 expression were observed in the spleen, and poly I:C was a stronger inducer. In addition, the maximal expression level of SmIRF-3 arose prior to that of the Mx in all the cases.
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China. huguobin@mail.ouc.edu.cn
authors.forename: Guo-Bin
authors.initials: GB
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Lou
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qiu-Ming
authors.initials: QM
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005412:Flatfishes / Q000235:genetics*; D040461:Gene Components; D005786:Gene Expression Regulation / Q000276:immunology*; D060226:Head Kidney / Q000378:metabolism; D050838:Interferon Regulatory Factor-3 / Q000235:genetics* / Q000378:metabolism*; D017994:Iridovirus / Q000276:immunology*; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D011070:Poly I-C / Q000276:immunology*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism
chemical_list: D017931:DNA Primers; D050838:Interferon Regulatory Factor-3; D012333:RNA, Messenger; D011070:Poly I-C