title: Mesenchymal stem cell delivery into rat infarcted myocardium using a porous polysaccharide-based scaffold: a quantitative comparison with endocardial injection.
abstract: The use of mesenchymal stem cells (MSCs) for tissue regeneration is often hampered by modest engraftment in host tissue. This study was designed to quantitatively compare MSCs engraftment rates after delivery using a polysaccharide-based porous scaffold or endocardial (EC) injection in a rat myocardial infarction model. Cellular engraftment was measured by quantitative reverse transcription-polymerase chain reaction using MSCs previously transduced with a lentiviral vector that expresses green fluorescent protein (GFP). The use of a scaffold promoted local cellular engraftment and survival. The number of residual GFP(+) cells was greater with the scaffold than after EC injection (9.7% vs. 5.1% at 1 month and 16.3% vs. 6.1% at 2 months, respectively [n=5]). This concurred with a significant increase in mRNA vascular endothelial growth factor level in the scaffold group (p<0.05). Clusters of GFP+ cells were detected in the peri-infarct area, mainly phenotypically consistent with immature MSCs. Functional assessment by echocardiography at 2 months postinfarct also showed a trend toward a lower left ventricular dilatation and a reduced fibrosis in the scaffold group in comparison to direct injection group (n=10). These findings demonstrate that using a porous biodegradable scaffold is a promising method to improve cell delivery and engraftment into damaged myocardium.
authors.affiliation: Inserm, U698, Bio-ingénierie Cardiovasculaire, Universities Paris 7 and Paris 13, Hôpital Bichat, Paris, France. catherine.levisage@inserm.fr
authors.forename: Catherine
authors.initials: C
authors.lastname: Le Visage
authors.forename: Olivier
authors.initials: O
authors.lastname: Gournay
authors.forename: Najah
authors.initials: N
authors.lastname: Benguirat
authors.forename: Sofiane
authors.initials: S
authors.lastname: Hamidi
authors.forename: Laeticia
authors.initials: L
authors.lastname: Chaussumier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Mougenot
authors.forename: James A
authors.initials: JA
authors.lastname: Flanders
authors.forename: Richard
authors.initials: R
authors.lastname: Isnard
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Stéphane
authors.initials: S
authors.lastname: Hatem
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Françoise
authors.initials: F
authors.lastname: Norol
mesh_terms: D000818:Animals; D006328:Cardiac Catheterization; D004562:Electrocardiography; D004699:Endocardium / Q000187:drug effects* / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007267:Injections; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D011134:Polysaccharides / Q000494:pharmacology*; D016062:Porosity / Q000187:drug effects; D019919:Prosthesis Implantation; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry*; D014463:Ultrasonography
chemical_list: D011134:Polysaccharides; D049452:Green Fluorescent Proteins