title: [Determination of trace sulfate ion in liposome doxorubicin with capillary electrophoresis].
abstract: A capillary electrophoretic method for the determination of the trace sulfate ion in liposome doxorubicin has been developed with potassium nitrate as the background electrolyte. The components and pH of the buffer solution, the concentration of electroosmotic flow modifier and the separation voltage on the separation were studied in detail. The optimized electrophoretic conditions were as follows: capillary column diameter 50 microm, total length 60 cm and effective length 51.5 cm, 20 mmol/L potassium nitrate buffer solution (pH 7.0) containing 0.4 mmol/L cetyltrimethylammonium chloride (CTAC) as the electroosmotic flow modifier, an applied voltage of -15 kV (reversed polarity) and a detection wavelength of 202 nm. Under the optimized capillary electrophoresis separation conditions, sulfate ion and chloride ion in the liposome doxorubicin breaking emulsion were separated successfully within 3 min, and the relative standard deviations of migration time and peak area of sulfate ion were less than 0.01% and 1.0% respectively. The detection limit was 5 microg/L. This method has been proved to be simple, sensitive and accurate, and also has been applied to determine sulfate ion in liposome doxorubicin sample with satisfactory results.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Zhan
authors.forename: Zhijia
authors.initials: Z
authors.lastname: Zhu
authors.forename: Jingwu
authors.initials: J
authors.lastname: Kang
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage*; D019075:Electrophoresis, Capillary / Q000379:methods*; D008081:Liposomes / Q000737:chemistry*; D013431:Sulfates / Q000032:analysis*
chemical_list: D000903:Antibiotics, Antineoplastic; D008081:Liposomes; D013431:Sulfates; D004317:Doxorubicin