title: RodZ regulates the post-transcriptional processing of the Shigella sonnei type III secretion system.
abstract: The expression of the type III secretion system-a main determinant of virulence in Shigella-is controlled by regulator cascades VirF-InvE (VirB) and CpxAR two-component system. A screen for mutants that restore virulence in the cpxA background led to the isolation of a mutant of rodZ, a cytoskeletal protein that maintains the rod-shaped morphology of bacilli. InvE is normally repressed at 30 °C because of decreased messenger RNA (mRNA) stability, but rodZ mutants markedly increase invE-mRNA stability. Importantly, the inhibition of InvE production by RodZ can be genetically separated from its role in cell-shape maintenance, indicating that these functions are distinguishable. Thus, we propose that RodZ is a new membrane-bound RNA-binding protein that provides a scaffold for post-transcriptional regulation.
authors.affiliation: Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku, Tokyo, Japan. jmitobe@nih.go.jp
authors.forename: Jiro
authors.initials: J
authors.lastname: Mitobe
authors.forename: Itaru
authors.initials: I
authors.lastname: Yanagihara
authors.forename: Kiyouhisa
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Shouji
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihama
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D055550:Protein Stability; D012323:RNA Processing, Post-Transcriptional; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012764:Shigella sonnei / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D012329:RNA, Bacterial; C085504:CpxR protein, Bacteria; D011494:Protein Kinases; C092472:CpxA protein, bacteria