title: Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate the pathological functions of OMVs from P. aeruginosa.
authors.affiliation: Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Choi
authors.forename: Dae-Kyum
authors.initials: DK
authors.lastname: Kim
authors.forename: Seng Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Jaewook
authors.initials: J
authors.lastname: Lee
authors.forename: Jun-Pyo
authors.initials: JP
authors.lastname: Choi
authors.forename: Sangchul
authors.initials: S
authors.lastname: Rho
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Yoon-Keun
authors.initials: YK
authors.lastname: Kim
authors.forename: Daehee
authors.initials: D
authors.lastname: Hwang
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D002853:Chromatography, Liquid; D055354:Exosomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D001425:Bacterial Outer Membrane Proteins; D020543:Proteome