title: Ghrelin stimulates myogenic differentiation in a mouse muscle satellite cell line and in primary cultures of bovine myoblasts.
abstract: Ghrelin is an acylated hormone that influences food intake, energy metabolism and reproduction, among others. Ghrelin may also stimulate proliferating myoblast cell differentiation and multinucleated myotube fusion. The aim of this work was to assess the effect of human ghrelin (hGHRL) and human ghrelin fragment 1-18 (hGHRL1-18) on myoblast differentiation by means of mRNA expression and protein level. Two types of cells were tested, the cell line i28 obtained from mouse skeletal muscle and primary cultures of bovine myoblasts. Both ghrelin and its N-terminal fragment hGHRL1-18 were used at concentrations of 0, 0.01, 0.1, 1, 10 and 100 nm. Treatments were applied to pre-confluent cultures and were maintained for 4 days. We determined that between 0.1 and 100 nm, hGHRL and hGRHL1-18 had similar effects on myogenic differentiation of i28 cells (p < 0.01). On the other hand, only the higher concentrations (10 and 100 nm) of hGHRL stimulated bovine myoblast differentiation. These results could be attributed to the presence, in both i28 cells and in bovine myoblasts, of the mRNA for GHS-R1a and CD36 receptors. The use of ghrelin in livestock production is still questionable because of the limited effects shown in this study, and additional research is needed in this field.
authors.affiliation: Programa de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Estado de México, México.
authors.forename: D
authors.initials: D
authors.lastname: Montoya-Flores
authors.forename: O
authors.initials: O
authors.lastname: Mora
authors.forename: E
authors.initials: E
authors.lastname: Tamariz
authors.forename: L
authors.initials: L
authors.lastname: González-Dávalos
authors.forename: A
authors.initials: A
authors.lastname: González-Gallardo
authors.forename: A
authors.initials: A
authors.lastname: Antaramian
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: A
authors.initials: A
authors.lastname: Varela-Echavarría
authors.forename: J L
authors.initials: JL
authors.lastname: Romano-Muñoz
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D054439:Ghrelin / Q000494:pharmacology*; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D054439:Ghrelin; D018008:Myogenin; D051763:PAX7 Transcription Factor