title: Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study.
abstract: A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction procedure consisting of deproteination and extraction with 3 parts of 6% trichloroacetic acid aqueous solution followed by volume make up with the aqueous component of the mobile phase obtained best recoveries of the two analytes. Samples were separated on a Supelco Discovery HS C(18) (150 mm × 4.6 mm, 5 μm) analytical column protected by a Perkin Elmer C(18) (30 mm × 4.6 mm, 10 μm) guard cartridge. The mobile phase, methanol/acetonitrile (50/50, v/v):0.05% trifluoroacetic acid (19:81, v/v), operated at 50°C column oven temperature was pumped at a flow rate of 2.0 mL min(-1) and the column eluents were monitored at a wavelength of 285 nm. When Sample was injected into the Perkin Elmer high performance liquid-chromatography system through Rheodyne manual (or auto-sampler) injector equipped with 20 μL loop, separation was achieved within 4 min. The present method demonstrated acceptable values for selectivity, linearity within the expected concentration range (0.004-5.0 μg mL(-1); r(2)>0.999 for both analytes), recovery (>95% for cefdinir and >96% for cefixime), precision (%RSD<2.0 for cefdinir and <2.2 for cefixime), sensitivity (limit of detection: 1 ng mL(-1) and lower limit of quantification: 4 ng mL(-1) for both analytes), stability of solutions, and robustness. The method was efficiently applied to a pharmacokinetic study in healthy volunteers.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar -25120, Pakistan.
authors.forename: Abbas
authors.initials: A
authors.lastname: Khan
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Khan
authors.forename: Khalid
authors.initials: K
authors.lastname: Javed
authors.forename: Abad
authors.initials: A
authors.lastname: Khan
authors.forename: Lateef
authors.initials: L
authors.lastname: Ahmad
authors.forename: Yasar
authors.initials: Y
authors.lastname: Shah
authors.forename: Fazli
authors.initials: F
authors.lastname: Nasir
mesh_terms: D000077525:Cefdinir; D020682:Cefixime / Q000097:blood* / Q000493:pharmacokinetics; D002511:Cephalosporins / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D006801:Humans
chemical_list: D002511:Cephalosporins; D020682:Cefixime; D000077525:Cefdinir