title: YGFP: a spectral variant of GFP.
abstract: We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
authors.affiliation: Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Flemming G
authors.initials: FG
authors.lastname: Hansen
authors.forename: Tove
authors.initials: T
authors.lastname: Atlung
mesh_terms: D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003062:Codon; D011994:Recombinant Proteins; C485192:green fluorescent protein, Aequorea victoria; D049452:Green Fluorescent Proteins