title: An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp. strain TTNP3.
abstract: In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the β- and γ-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu.
authors.affiliation: Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences Northwestern Switzerland, Muttenz, Switzerland. boris.kolvenbach@fhnw.ch
authors.forename: Boris A
authors.initials: BA
authors.lastname: Kolvenbach
authors.forename: Hyazinth
authors.initials: H
authors.lastname: Dobrowinski
authors.forename: Jan
authors.initials: J
authors.lastname: Fousek
authors.forename: Cestmir
authors.initials: C
authors.lastname: Vlcek
authors.forename: Andreas
authors.initials: A
authors.lastname: Schäffer
authors.forename: Frederic L P
authors.initials: FL
authors.lastname: Gabriel
authors.forename: Hans-Peter E
authors.initials: HP
authors.lastname: Kohler
authors.forename: Philippe F X
authors.initials: PF
authors.lastname: Corvini
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005798:Genes, Bacterial; D006873:Hydroquinones / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D017422:Sequence Analysis, DNA; D020578:Sphingomonas / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D006873:Hydroquinones; D010636:Phenols; C002091:hydroxymuconic semialdehyde; D010088:Oxidoreductases; D049308:Dioxygenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C021530:maleylacetate reductase; C031927:hydroquinone