title: [Effect of sulfide to nitrate ratios on the strains coupling nitrogen removal from wastewater and hydrogen sulfide removal from biogas].
abstract: Two strains Pseudomonas fluorescens and Pseudomonas aeruginosa were isolated from the reactors coupling nitrogen removal from wastewater and hydrogen sulfide removal from biogas. It was investigated that sulfide to nitrate ratios had effects on the simultaneous removal of sulfide and nitrate of the two strains under the conditions of pH 7.0, temperature 30 degrees C, anaerobic, cultured for 2 days. The results showed that the nitrate-nitrogen removal rate of Pseudomonas aeruginosa was higher than that of Pseudomonas fluorescens under the same conditions. The sulfide-sulfur removal rates of the two strains had little difference. The best molar n(S)/n(N) of sulfide and nitrate removal rates of Pseudomonas fluorescens and Pseudomonas aeruginosa were 5/2-5/3, 5/3-5/4, respectively. Pseudomonas fluorescens could transform NO3- -N into NO2-N fast, its principal NOx- -N not removed was NO2- -N, especially at the low initial concentration of NOx- -N. The NOx- -N not removed was NO3- -N by Pseudomonas aeruginosa. The best molar n(S)/n(N) of sulfur as reaction outcome of sulfide-sulfur were 5/4, 5/3 by Pseudomonas fluorescens and Pseudomonas aeruginosa, respectively.
authors.affiliation: Biogas Scientific Research Institute of Ministry of Agriculture,Chengdu 610041, China. cza0903@yahoo.com.cn
authors.forename: Zi-ai
authors.initials: ZA
authors.lastname: Chen
authors.forename: Liang-wei
authors.initials: LW
authors.lastname: Deng
authors.forename: Li
authors.initials: L
authors.lastname: He
mesh_terms: D001673:Biodegradation, Environmental; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D006862:Hydrogen Sulfide / Q000302:isolation & purification*; D009566:Nitrates / Q000032:analysis; D009584:Nitrogen / Q000302:isolation & purification*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013440:Sulfides / Q000032:analysis; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D056804:Biofuels; D009566:Nitrates; D013440:Sulfides; D009584:Nitrogen; D006862:Hydrogen Sulfide