title: Bioluminescent stem-loop probes for highly sensitive nucleic acid detection.
abstract: Here, we report the first bioluminescent stem-loop probe, which is 50 times more sensitive and able to achieve a LOD 25 times lower than fluorescent stem-loop probes. Chemical generation of a signal from Renilla luciferase reduces background noise for improved quantitative utility in nucleic acid biomarker detection.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Hunt
authors.forename: Sapna K
authors.initials: SK
authors.lastname: Deo
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry; D049410:Luciferases, Renilla / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis*; D017434:Protein Structure, Tertiary
chemical_list: D005456:Fluorescent Dyes; D009696:Nucleic Acids; D049410:Luciferases, Renilla