title: Increasing the antibacterial effect of lysozyme by immobilization on multi-walled carbon nanotubes.
abstract: We report a facile strategy to obtain multiwalled carbon nanotubes (MWCNTs) functionalized with covalently bonded lysozyme. The functionalization procedure has been investigated by means of several techniques, including thermogravimetry, Raman spectroscopy, transmission electron microscopy, and cyclic voltammetry. A functionalization of about 1 lysozyme molecule every 4000 carbon atoms is obtained. The modified lysozyme-CNTs nanocomposite shows a significant increase of the antibacterial activity towards the Gram-positive S. aureus if compared with lysozyme in solution.
authors.affiliation: Department of General Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Merli
authors.forename: Monica
authors.initials: M
authors.lastname: Ugonino
authors.forename: Antonella
authors.initials: A
authors.lastname: Profumo
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fagnoni
authors.forename: Eliana
authors.initials: E
authors.lastname: Quartarone
authors.forename: Piercarlo
authors.initials: P
authors.lastname: Mustarelli
authors.forename: Livia
authors.initials: L
authors.lastname: Visai
authors.forename: Marco S
authors.initials: MS
authors.lastname: Grandi
authors.forename: Pietro
authors.initials: P
authors.lastname: Galinetto
authors.forename: Patrizia
authors.initials: P
authors.lastname: Canton
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000494:pharmacology; D009113:Muramidase / Q000737:chemistry* / Q000494:pharmacology*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D009113:Muramidase