title: A black soybean seed coat extract prevents obesity and glucose intolerance by up-regulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diet-fed mice.
abstract: Black soybean seed coat extract (BE) is a polyphenol-rich food material consisting of 9.2% cyanidin 3-glucoside, 6.2% catechins, 39.8% procyanidins, and others. This study demonstrated that BE ameliorated obesity and glucose intolerance by up-regulating uncoupling proteins (UCPs) and down-regulating inflammatory cytokines in C57BL/6 mice fed a control or high-fat diet containing BE for 14 weeks. BE suppressed fat accumulation in mesenteric adipose tissue, reduced the plasma glucose level, and enhanced insulin sensitivity in the high-fat diet-fed mice. The gene and protein expression levels of UCP-1 in brown adipose tissue and UCP-2 in white adipose tissue were up-regulated by BE. Moreover, the gene expression levels of major inflammatory cytokines, tumor necrosis factor-α and monocyte chemoattractant protein-1 were remarkably decreased by BE in white adipose tissue. BE is a beneficial food material for the prevention of obesity and diabetes by enhancing energy expenditure and suppressing inflammation.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kanamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Fumio
authors.initials: F
authors.lastname: Nanba
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takanori
authors.initials: T
authors.lastname: Tsuda
authors.forename: Itsuko
authors.initials: I
authors.lastname: Fukuda
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nakamura-Tsuruta
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D002001:Adipose Tissue, Brown / Q000737:chemistry; D052436:Adipose Tissue, White / Q000737:chemistry; D000818:Animals; D016207:Cytokines / Q000032:analysis* / Q000235:genetics; D015536:Down-Regulation; D018149:Glucose Intolerance / Q000517:prevention & control*; D007249:Inflammation; D007473:Ion Channels / Q000032:analysis / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins / Q000032:analysis / Q000235:genetics; D009765:Obesity / Q000517:prevention & control*; D010936:Plant Extracts / Q000008:administration & dosage*; D012639:Seeds / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*; D014475:Uncoupling Agents / Q000032:analysis*; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D007473:Ion Channels; D024101:Mitochondrial Proteins; D010936:Plant Extracts; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2