title: mRNA expression profiles of P450 3A enzymes in the liver and small intestine of the domestic pig.
abstract: The cytochrome P450 (P450) 3A family is considered to be the most important and abundantly expressed P450 subfamily in mammals. The mRNA expression levels of four P450 3A enzymes in porcine liver and small intestine were investigated using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The expression of P450 3A mRNAs (P450 3A pool) was higher in the liver than that in the small intestine. In the small intestine, the P450 3A mRNAs were gradually decreased from the duodenum to the ileum. P450 3A29 and P450 3A22 were predominantly expressed both in liver and small intestine tissues with larger ratios in the P450 3A pool than the other P450 3A enzymes. These results demonstrate that P450 3A29 and P450 3A22 probably serve as the major P450 3A contributors for both the hepatic and intestinal P450 3A pool. This work provides a deeper comprehension of the contribution of P450 3A enzymes to xenobiotic metabolism in pigs.
authors.affiliation: MAO Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.forename: Menghong
authors.initials: M
authors.lastname: Dai
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Liu
authors.forename: Wenlong
authors.initials: W
authors.lastname: Cui
authors.forename: Daoyuan
authors.initials: D
authors.lastname: Li
authors.forename: Huahai
authors.initials: H
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yahui
authors.initials: Y
authors.lastname: Liu
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000818:Animals; D051544:Cytochrome P-450 CYP3A / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000201:enzymology; D015870:Gene Expression; D007082:Ileum / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine
chemical_list: D012333:RNA, Messenger; D051544:Cytochrome P-450 CYP3A