title: [Relationship between orthodontics root resorption following experimental tooth movement and the level of dentin sialoph-osphoprotein and dentin sialoprotein in gingival crevicular fluid].
abstract: OBJECTIVE
  To investigate the relationship of expression of dentin sialoph-osphoprotein (DSPP) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) with root resorption following experimental tooth movement in rats.
  
  
  METHODS
  36 Wistar rats were divided into 3 groups on average randomly: Control group, light force group and heavy force group. The experimental teeth were drawn-off mesially by the force of 0.392 N in light force group and 0.98 N in heavy force group, with both of the maxillary central incisors as the tooth of anchorage. At the 7th day, the gingival crevicular fluid of rats were collected; the histological slices were made, including the experimental tooth and periodontal tissue; the tissues was stained with hematoxylin-eosin (HE) staining and tartrate resistant acid phosphatase (TRAP) staining to observe the histological changes of the root resorption of rats. Then the expression of DSPP and DSP were assayed by using biochemistry techniques of Western blot.
  
  
  RESULTS
  Histological observation: There was not root resorption in control group. Neither root resorption nor cementoclast was observed in light force group. And in heavy force group visible root resorption came out in pressure zone. Western blot results: There was expression of DSPP and no DSP in control group, and there was the expression of DSPP and DSP in both light force group and heavy force group. The result of statistical analysis showed that there were significant differences in the expression of DSPP and DSP among three groups. The highest one was heavy force group, followed by the light force group and control group with the least amount of proteins.
  
  
  CONCLUSIONS
  There is the expression of DSPP and DSP in gingival crevicular fluid following experimental tooth movement with root resorption.
authors.affiliation: Dept. of Orthodontics, College of Stomatology, Jilin University, Changchun 130021, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Zuo
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Huan
authors.initials: H
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Tian
mesh_terms: D000818:Animals; D003804:Dentin; D016326:Extracellular Matrix Proteins; D005883:Gingival Crevicular Fluid; D007180:Incisor; D008297:Male; D009970:Orthodontics; D010750:Phosphoproteins; D051381:Rats; D017208:Rats, Wistar; D012391:Root Resorption; D012795:Sialoglycoproteins; D014087:Tooth Movement Techniques; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein