title: MicroRNA-19b downregulates insulin 1 through targeting transcription factor NeuroD1.
abstract: MiR-17-92 cluster miRNAs are disclosed to contribute to the development of multiple organs and tumorigenesis, but their roles in pancreas development remains unclear. In this study, we found that miR-19b, a member of miR-17-92, was highly expressed in the pancreatic progenitor cells, and miR-19b could target the 3' UTR of NeuroD1 mRNA to decrease its protein and mRNA levels. Functional analysis showed that miR-19b exerted little effect on the proliferation of pancreatic progenitors, whereas it inhibited the expression of insulin 1, but not insulin 2 in MIN6 cells. These results suggest that miR-19b can downregulate insulin 1 expression through targeting transcription factor NeuroD1, and thus regulate the differentiation and function of β-cells.
authors.affiliation: Laboratory of Animal Development Biology, College of Life Science, Northeast Forestry University, Harbin, China.
authors.forename: Zhen-Wu
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Ding
authors.forename: Fa
authors.initials: F
authors.lastname: Wang
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: An
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Chun-Bo
authors.initials: CB
authors.lastname: Teng
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D007328:Insulin / Q000235:genetics*; D050417:Insulin-Secreting Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D007328:Insulin; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C561642:MIRN19 microRNA, mouse; C093564:Neurogenic differentiation factor 1