title: Autoimmunity to endoplasmic reticulum chaperone GRP94 in myasthenia gravis.
abstract: Immune responses to ER stress have been closely related to the pathogenesis of autoimmune diseases. Using an immunoprecipitation assay, 24 (7.1%) of 336 MG serum samples immunoprecipitated a 90-kDa protein from the muscle cellular extracts, but none of the disease or healthy control sera. The 90-kDa protein was affinity-purified and found to match to ER chaperon GRP94 by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy analysis. The frequency of associated autoimmune diseases was much higher in the anti-GRP94-positive than the -negative patients (71% versus 11%, p<0.001). Autoimmunity to ER chaperone GRP94 is associated with a subset of MG patients who have additional autoimmune diseases.
authors.affiliation: Department of Neurology, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan. sgsuzuki@z3.keio.jp
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kimiaki
authors.initials: K
authors.lastname: Utsugisawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Iwasa
authors.forename: Takashi
authors.initials: T
authors.lastname: Satoh
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Nagane
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Masataka
authors.initials: M
authors.lastname: Kuwana
authors.forename: Norihiro
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood; D001324:Autoantigens / Q000096:biosynthesis / Q000097:blood; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology*; D008875:Middle Aged; D018832:Molecular Chaperones / Q000276:immunology*; D009157:Myasthenia Gravis / Q000276:immunology* / Q000378:metabolism*; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; C053387:endoplasmin