title: Skin-resident murine dendritic cell subsets promote distinct and opposing antigen-specific T helper cell responses.
abstract: Skin-resident dendritic cells (DCs) are well positioned to encounter cutaneous pathogens and are required for the initiation of adaptive immune responses. There are at least three subsets of skin DC- Langerhans cells (LC), Langerin(+) dermal DCs (dDCs), and classic dDCs. Whether these subsets have distinct or redundant function in vivo is poorly understood. Using a Candida albicans skin infection model, we have shown that direct presentation of antigen by LC is necessary and sufficient for the generation of antigen-specific T helper-17 (Th17) cells but not for the generation of cytotoxic lymphocytes (CTLs). In contrast, Langerin(+) dDCs are required for the generation of antigen specific CTL and Th1 cells. Langerin(+) dDCs also inhibited the ability of LCs and classic DCs to promote Th17 cell responses. This work demonstrates that skin-resident DC subsets promote distinct and opposing antigen-specific responses.
authors.affiliation: Department of Dermatology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
authors.forename: Botond Z
authors.initials: BZ
authors.lastname: Igyártó
authors.forename: Krystal
authors.initials: K
authors.lastname: Haley
authors.forename: Daniela
authors.initials: D
authors.lastname: Ortner
authors.forename: Aleh
authors.initials: A
authors.lastname: Bobr
authors.forename: Maryam
authors.initials: M
authors.lastname: Gerami-Nejad
authors.forename: Brian T
authors.initials: BT
authors.lastname: Edelson
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Zurawski
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.forename: Gerard
authors.initials: G
authors.lastname: Zurawski
authors.forename: Judith
authors.initials: J
authors.lastname: Berman
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Kaplan
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D002176:Candida albicans / Q000276:immunology* / Q000472:pathogenicity; D002177:Candidiasis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D002478:Cells, Cultured; D045142:Cross-Priming; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D037181:Lectins, C-Type / Q000096:biosynthesis; D008213:Lymphocyte Activation; D037241:Mannose-Binding Lectins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D012867:Skin / Q000382:microbiology / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000469:parasitology; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D050976:Basic-Leucine Zipper Transcription Factors; C498497:Cd207 protein, mouse; D037181:Lectins, C-Type; D037241:Mannose-Binding Lectins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012097:Repressor Proteins; C533289:SNFT protein, mouse