title: Interactions between subunits of protein kinase CK2 and their protein substrates influences its sensitivity to specific inhibitors.
abstract: Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α', CK2α and three holoenzymatic structures composed of αα'ββ', α(2)ββ' and α'(2)ββ'. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to specific modulators. All five isoforms of protein kinase CK2 from Saccharomyces cerevisiae were examined for their binding capacity with ATP/GTP and two commonly used ATP-competitive inhibitors TBB and TBI. Enzymes were tested with protein substrates differently interacting with CK2 subunits: Elf1, Fip1, Svf1, P2B and synthetic peptide. Obtained results show that K(m) for ATP varies from 2.4-53 μM for Elf1/CK2α' and Svf1/CK2α, respectively. Similar differences can be seen in case when GTP was used as phosphate donor. The inhibitory effect depends on composition of CK2/substrate complexes. Highest sensitivity to TBB shows all complexes containing αα'ββ' isoform with K (i) values between 0.2 and 1.1 μM. The prospect that TBB and TBI could be utilized to discriminate between different molecular forms of CK2 in yeast cells was examined. Both inhibitors, TBB as well as TBI, decreases cell growth to extents devoting interactions with different CK2 isoforms present in the cell but the presence of β/β'-dimer has a high importance towards sensitivity. Conceivably, a given inhibitor concentration can inhibit only selected CK2-mediated processes in the cell.
authors.affiliation: Department of Molecular Biology, Institute of Biotechnology, John Paul II Catholic University of Lublin, Kraśnicka Av. 102, 20-718 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Janeczko
authors.forename: Maciej
authors.initials: M
authors.lastname: Masłyk
authors.forename: Ryszard
authors.initials: R
authors.lastname: Szyszka
authors.forename: Andrea
authors.initials: A
authors.lastname: Baier
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D050296:Microbial Viability / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D007527:Isoenzymes; D010710:Phosphates; D047428:Protein Kinase Inhibitors; D021122:Protein Subunits; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D047390:Casein Kinase II