title: Glutathione peroxidase 2 in Saccharomyces cerevisiae is distributed in mitochondria and involved in sporulation.
abstract: Gpx2, one of three glutathione peroxidase homologs (Gpx1, Gpx2, and Gpx3) in Saccharomyces cerevisiae, is an atypical 2-Cys peroxiredoxin that prefers to use thioredoxin as a reducing agent in vitro. Despite Gpx2 being an antioxidant, no obvious phenotype of gpx2Δ mutant cells in terms of oxidative stress has yet been found. To gain a clue as to Gpx2's physiological function in vivo, here we identify its intracellular distribution. Gpx2 was found to exist in the cytoplasm and mitochondria. In mitochondria, Gpx2 was associated with the outer membrane of the cytoplasmic-side, as well as the inner membrane of the matrix-side. The redox state of the mitochondrial Gpx2 was regulated by Trx1 and Trx2 (cytoplasmic thioredoxin), and by Trx3 (mitochondrial matrix thioredoxin). In addition, we found that the disruption of GPX2 reduced the sporulation efficiency of diploid cells.
authors.affiliation: Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Yuuta
authors.initials: Y
authors.lastname: Ukai
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kishimoto
authors.forename: Takumi
authors.initials: T
authors.lastname: Ohdate
authors.forename: Singo
authors.initials: S
authors.lastname: Izawa
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D003593:Cytoplasm / Q000201:enzymology; D005979:Glutathione Peroxidase / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D010084:Oxidation-Reduction; D054464:Peroxiredoxins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism / Q000502:physiology*; D013172:Spores, Fungal / Q000201:enzymology / Q000502:physiology*; D013879:Thioredoxins / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C103243:TRX1 protein, S cerevisiae; C516110:TRX2 protein, S cerevisiae; D013879:Thioredoxins; C492173:GPX2 protein, S cerevisiae; D054464:Peroxiredoxins; D005979:Glutathione Peroxidase