title: Transformation frequency of a mariner-based transposon in Rickettsia rickettsii.
abstract: Transformation frequencies of a mariner-based transposon system in Rickettsia rickettsii were determined using a plaque assay system for enumeration and isolation of mutants. Sequence analysis of insertion sites in both R. rickettsii and R. prowazekii indicated that insertions were random. Transposon mutagenesis provides a useful tool for rickettsial research.
authors.affiliation: Host-Parasite Interactions Section, Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 598401, USA.
authors.forename: Tina R
authors.initials: TR
authors.lastname: Clark
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Lackey
authors.forename: Betsy
authors.initials: B
authors.lastname: Kleba
authors.forename: Lonnie O
authors.initials: LO
authors.lastname: Driskell
authors.forename: Erika I
authors.initials: EI
authors.lastname: Lutter
authors.forename: Craig
authors.initials: C
authors.lastname: Martens
authors.forename: David O
authors.initials: DO
authors.lastname: Wood
authors.forename: Ted
authors.initials: T
authors.lastname: Hackstadt
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016254:Mutagenesis, Insertional; D012283:Rickettsia prowazekii / Q000235:genetics; D012284:Rickettsia rickettsii / Q000235:genetics*; D014170:Transformation, Genetic; D010948:Viral Plaque Assay
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial