title: Use of a novel virus detection assay to identify coronavirus HKU1 in the lungs of a hematopoietic stem cell transplant recipient with fatal pneumonia.
abstract: A 38-year-old female patient with systemic lupus erythematosus presented with pulmonary infiltrates and hypoxemia for several months following immunodepleting autologous hematopoietic stem cell transplantation. She was treated for influenza, which was isolated repeatedly from oropharynx and bronchoalveolar lavage (BAL) fluids, and later empirically for lupus pneumonitis, but died 6 months after transplant. Autopsy findings failed to show influenza in the lungs or lupus pneumonitis. A novel generic polymerase chain reaction (PCR)-based assay using degenerate primers identified human coronavirus (CoV) HKU1 RNA in BAL fluid at autopsy. CoV was confirmed by virus-specific PCRs of lung tissue at autopsy. Electron microscopy showed viral particles consistent with CoV HKU1 in lung tissue both at autopsy and from a previous biopsy. Although human CoV HKU1 infection is not usually severe, in highly immunocompromised patients, it can be associated with fatal pneumonia.
authors.affiliation: Division of Viral Products, Center for Biologics, Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
authors.forename: C
authors.initials: C
authors.lastname: Uhlenhaut
authors.forename: J I
authors.initials: JI
authors.lastname: Cohen
authors.forename: S
authors.initials: S
authors.lastname: Pavletic
authors.forename: G
authors.initials: G
authors.lastname: Illei
authors.forename: J C
authors.initials: JC
authors.lastname: Gea-Banacloche
authors.forename: M
authors.initials: M
authors.lastname: Abu-Asab
authors.forename: T
authors.initials: T
authors.lastname: Krogmann
authors.forename: L
authors.initials: L
authors.lastname: Gubareva
authors.forename: S
authors.initials: S
authors.lastname: McClenahan
authors.forename: P R
authors.initials: PR
authors.lastname: Krause
mesh_terms: D000328:Adult; D001344:Autopsy; D001706:Biopsy; D017934:Coronavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000821:virology*; D017809:Fatal Outcome; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008168:Lung / Q000821:virology*; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods