title: Bacillus subtilis (DSM17299) significantly reduces Salmonella in broilers.
abstract: Salmonella continues to be a major public health burden worldwide. Poultry are known to be one of the main reservoirs for this zoonotic pathogen. It has previously been shown that a single dose of Bacillus subtilis reduces fecal shedding of Salmonella enterica serovar Enteritidis, whereas no effect on long-term colonization of the cecum has been observed. Here we report experiments that were undertaken to test the efficacy of a conventional diet supplemented with a probiotic (B. subtilis DSM17299) on 1) Salmonella colonization in the intestinal tract of broiler chickens, and 2) fecal shedding of Salmonella under production-like conditions. The trial birds fed the B. subtilis diet showed a significant 58% reduction in Salmonella-positive drag swabs compared with control birds, which had 100% presence of Salmonella. Feeding B. subtilis significantly reduced the average Salmonella load of cecum samples of the chickens, by 3 log units. This reduction in Salmonella colonization might not only positively affect broilers on the live production side by reducing the risk of infection between birds, but could also aid on the processing side by decreasing the amount of Salmonella entering the facility and improving food safety. Furthermore, numerical, but not statistically significant, improvements in feed conversion rate and BW gain at d 42 were observed in the B. subtilis-treated group compared with control birds.
authors.affiliation: Chr. Hansen A/S, Boege Alle 10-12, 2970 Hoersholm, Denmark.
authors.forename: I
authors.initials: I
authors.lastname: Knap
authors.forename: A B
authors.initials: AB
authors.lastname: Kehlet
authors.forename: M
authors.initials: M
authors.lastname: Bennedsen
authors.forename: G F
authors.initials: GF
authors.lastname: Mathis
authors.forename: C L
authors.initials: CL
authors.lastname: Hofacre
authors.forename: B S
authors.initials: BS
authors.lastname: Lumpkins
authors.forename: M M
authors.initials: MM
authors.lastname: Jensen
authors.forename: M
authors.initials: M
authors.lastname: Raun
authors.forename: A
authors.initials: A
authors.lastname: Lay
mesh_terms: D000821:Animal Feed; D000818:Animals; D001412:Bacillus subtilis / Q000145:classification* / Q000502:physiology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004032:Diet / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005516:Food Microbiology; D005766:Gastrointestinal Contents / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology* / Q000517:prevention & control; D019936:Probiotics / Q000494:pharmacology; D012475:Salmonella / Q000502:physiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology* / Q000517:prevention & control