title: ¹H, ¹⁵N and ¹³C backbone chemical shift assignment of the titin A67-A68 domain tandem.
abstract: Single molecules of the giant protein titin extend across half of the muscle sarcomere, from the Z-line to the M-line, and have roles in muscle assembly and elasticity. In the A-band titin is attached to thick filaments and here the domain arrangement occurs in regular patterns of eleven called the large super-repeat. The large super-repeat itself occurs eleven times and forms nearly half the titin molecule. Interactions of the large super-repeats with myosin are consistent with a role in thick filament assembly. Here we report backbone assignments of the titin A67-A68 domain tandem (Fn-Ig) from the third super-repeat (A65-A75) completed using triple resonance NMR experiments.
authors.affiliation: Institute for Molecular and Cellular Biology and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: András
authors.initials: A
authors.lastname: Czajlik
authors.forename: Gary S
authors.initials: GS
authors.lastname: Thompson
authors.forename: Ghulam N
authors.initials: GN
authors.lastname: Khan
authors.forename: Arnout P
authors.initials: AP
authors.lastname: Kalverda
authors.forename: Steve W
authors.initials: SW
authors.lastname: Homans
authors.forename: John
authors.initials: J
authors.lastname: Trinick
mesh_terms: D064211:Connectin; D009124:Muscle Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011494:Protein Kinases / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D020449:Repetitive Sequences, Amino Acid
chemical_list: D064211:Connectin; D009124:Muscle Proteins; D011494:Protein Kinases