title: Molecular imaging of brain lipid environment of lymphocytes in amyotrophic lateral sclerosis using magnetic resonance imaging and SECARS microscopy.
abstract: This paper highlights some of the key technologies of using two innovative molecular imaging modalites, magnetic resonance imaging (MRI) and nonlinear optical microscopy, for imaging intravenously injected ultra small paramagnetic iron oxide nanoparticles cross linked with antibodies (CLUSPIO) in the amyotrophic lateral sclerosis (ALS) experimental model in vivo or ex vivo, respectively. Intensive efforts have been made in investigating the causes of abnormalities in lipid metabolism, monitored in some neurodegenerative disorders systems. It has been shown that an abnormal accumulation of some common lipids in motor nerve cells may play a critical role in the development of amyotrophic lateral sclerosis. The presented experiments were performed on brain specimens from the transgenic rat model expressing multiple copies of mutated (G93A) human SOD-1 gene, after CD4+ lymphocytes were magnetically labeled with i.v.i. CLUSPIO antibodies. In vivo MRI revealed marked signal intensity enhancements in specific pathological regions of the ALS rat brain as compared to the wild type. Surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy indicated cellular interactions based on lipids association to anti-CD4 CLUSPIO.
authors.affiliation: Universitätsklinik für Radiodiagnostik, Innsbruck Medical University, Innsbruck, Austria. lina.machtoub@i-med.ac.at
authors.forename: L
authors.initials: L
authors.lastname: Machtoub
authors.forename: D
authors.initials: D
authors.lastname: Bataveljić
authors.forename: P R
authors.initials: PR
authors.lastname: Andjus
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D000906:Antibodies; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D003287:Contrast Media; D003911:Dextrans; D004195:Disease Models, Animal; D006801:Humans; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008853:Microscopy; D057054:Molecular Imaging / Q000379:methods*; D009154:Mutation; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D013059:Spectrum Analysis, Raman; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1
chemical_list: D000906:Antibodies; D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; C000606290:SOD1 protein, human; C097921:ferumoxtran-10; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1