title: Using complementary DNA from MyoD-transduced fibroblasts to sequence large muscle genes.
abstract: Large muscle genes are often sequenced using complementary DNA (cDNA) made from muscle messenger RNA (mRNA) to reduce the cost and workload associated with sequencing from genomic DNA. Two potential barriers are the availability of a frozen muscle biopsy, and difficulties in detecting nonsense mutations due to nonsense-mediated mRNA decay (NMD). We present patient examples showing that use of MyoD-transduced fibroblasts as a source of muscle-specific mRNA overcomes these potential difficulties in sequencing large muscle-related genes.
authors.affiliation: Institute for Neuroscience and Muscle Research, Discipline of Paediatrics and Child Health, Children's Hospital at Westmead, University of Sydney, Locked Bag 4001, Westmead, New South Wales 2145, Australia.
authors.forename: Leigh B
authors.initials: LB
authors.lastname: Waddell
authors.forename: Nicole
authors.initials: N
authors.lastname: Monnier
authors.forename: Sandra T
authors.initials: ST
authors.lastname: Cooper
authors.forename: Kathryn N
authors.initials: KN
authors.lastname: North
authors.forename: Nigel F
authors.initials: NF
authors.lastname: Clarke
mesh_terms: D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger