title: [A novel method for multiplex detection of gastroenteritis-associated viruses].
abstract: To develop and optimize a simultaneous detection method of RotavirusA, Norovirus GI, GII, Sapovirus, human astrovirus, enteric adenoviruses and HBoV2 with GenomeLab GeXP analysis system. The sensitivity was verified to be 10(4) copies/microL with plasmids containing the viral targets in triplicate on different days, and no cross-reaction with enterovirus71, human Parechovirus and PicobirnavirusII was observed. Finally, we successfully developed a high throughout, rapid and maneuverable multiplex RT-PCR assay for simultaneous detection of seven viruses related with viral gastroenteritis, which provide a novel method for the molecular diagnosis of diarrhea-associated virus.
authors.affiliation: State Key Laboratory of Molecular Virology and Genetic Engineering, Institute of Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zi-Qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Xun
authors.initials: X
authors.lastname: Gong
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Li
authors.forename: Wan-Zhu
authors.initials: WZ
authors.lastname: Yang
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Yang
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014780:Viruses / Q000302:isolation & purification*