title: Biotransformation of dehydroepiandrosterone with Macrophomina phaseolina and β-glucuronidase inhibitory activity of transformed products.
abstract: The biotransformation of dehydroepiandrosterone (1) with Macrophomina phaseolina was investigated. A total of eight metabolites were obtained which were characterized as androstane-3,17-dione (2), androst-4-ene-3,17-dione (3), androst-4-ene-17β-ol-3-one (4), androst-4,6-diene-17β-ol-3-one (5), androst-5-ene-3β,17β-diol (6), androst-4-ene-3β-ol-6,17-dione (7), androst-4-ene-3β,7β,17β-triol (8), and androst-5-ene-3β,7α,17β-triol (9). All the transformed products were screened for enzyme inhibition, among which four were found to inhibit the β-glucuronidase enzyme, while none inhibited the α-chymotrypsin enzyme.
authors.affiliation: H. E. J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. hej@cyber.net.pk
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
authors.forename: Salman
authors.initials: S
authors.lastname: Zafar
authors.forename: Naik Tameen
authors.initials: NT
authors.lastname: Khan
authors.forename: Saeed
authors.initials: S
authors.lastname: Ahmad
authors.forename: Shagufta
authors.initials: S
authors.lastname: Noreen
authors.forename: Bishnu P
authors.initials: BP
authors.lastname: Marasini
authors.forename: Abdulaziz A
authors.initials: AA
authors.lastname: Al-Khedhairy
authors.lastname: Atta-Ur-Rahman
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D001711:Biotransformation; D003687:Dehydroepiandrosterone / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D005966:Glucuronidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D003687:Dehydroepiandrosterone; D005966:Glucuronidase