title: Transient model of thermal deactivation of enzymes.
abstract: The kinetics of enzyme deactivation provide useful insights on processes that determine the level of biological function of any enzyme. Photinus pyralis (firefly) luciferase is a convenient enzyme system for studying mechanisms and kinetics of enzyme deactivation, refolding, and denaturation caused by various external factors, physical or chemical by nature. In this report we present a study of luciferase deactivation caused by increased temperature (i.e., thermal deactivation). We found that deactivation occurs through a reversible intermediate state and can be described by a Transient model that includes active and reversibly inactive states. The model can be used as a general framework for analysis of complex, multiexponential transient kinetics that can be observed for some enzymes by reaction progression assays. In this study the Transient model has been used to develop an analytical model for studying a time course of luciferase deactivation. The model might be applicable toward enzymes in general and can be used to determine if the enzyme exposed to external factors, physical or chemical by nature, undergoes structural transformation consistent with thermal mechanisms of deactivation.
authors.affiliation: Sci-Tec, Knoxville, TN, USA. ngchen@aurogene.com
authors.forename: Nelson G
authors.initials: NG
authors.lastname: Chen
authors.forename: Kalvin
authors.initials: K
authors.lastname: Gregory
authors.forename: Ye
authors.initials: Y
authors.lastname: Sun
authors.forename: Val
authors.initials: V
authors.lastname: Golovlev
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D004795:Enzyme Stability / Q000502:physiology*; D048888:Fireflies / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D049409:Luciferases, Firefly / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D011489:Protein Denaturation; D013696:Temperature; D013997:Time Factors
chemical_list: D049409:Luciferases, Firefly