title: [Protection against acute hypoxic/reoxygenation injury to kidney for rabbit with morphine hypoxic preconditioning by observing the expression of caspase-3 protein].
abstract: The maintenance of the balance between oxygen supply and oxygen consumption is a key measure in preventing acute kidney hypoxic/reoxygenation injury. Morphine can inhibit metabolism and reduce the oxygen consumption. We tried to investigate the protective effects of morphine hypoxic preconditioning on acute kidney hypoxic/reoxygenation injury in rabbit and its influence on expression of caspase-3 protein. Kidney hypoxic and reoxygenation were induced by making the tested rabbits inhale 8% oxygen for three hours firstly, and then putting them in the air to breathe in normal oxygen for another three hours. Morphine hypoxic preconditioning was induced by administering morphine 3 mg/kg, and then hypoxic of 8% oxygen was induced. Caspase-3 protein expression in renal tissue was assessed by immunohistochemical method. In the present study, the expressions of caspase-3 protein were significantly higher in saline-control hypoxic group than in morphine hypoxic preconditioning group ((29.3+/-5.7)% vs. (12.16+1.23)%, P<0.05). These observations suggested that morphine hypoxic preconditioning can protect rabbit against acute kidney hypoxic/reoxygenation injury by decreasing expression of caspase-3 protein.
authors.affiliation: Department of Anesthesiology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Jiang
authors.forename: Juan
authors.initials: J
authors.lastname: Ni
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Luo
authors.forename: Huafeng
authors.initials: H
authors.lastname: Li
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D009020:Morphine; D013482:Superoxide Dismutase; D053148:Caspase 3