title: Anti-osteoporosis activity of Cibotium barometz extract on ovariectomy-induced bone loss in rats.
abstract: BACKGROUND
  Recent research has confirmed that Cibotium barometz could inhibits osteoclast formation with no affect on BMM cell viability. However, the influence of Cibotium barometz on osteoporosis in animals is relatively unknown. The purpose of this study is to systemically investigate the effects of Cibotium barometz extract (CBE) on ovariectomy-induced osteoporosis in rats.
  
  
  METHODS
  A total of Seventy-two 3-month-old female Sprague-Dawley rats were used and randomly divided into sham-operated group and five ovariectomized (OVX) groups: OVX with vehicle; OVX with 17β-estradiol (E2, 25 μg/kg/day); OVX with CBE of graded doses (100, 300, or 500 mg/kg/day). Daily oral administration of E2 or CBE began 4 weeks after the surgery and lasted for 16 weeks. Bone mass, bone turnover and strength were analyzed by DEXA, biochemical markers and three-point bending test. The trabecular bone microarchitecture was evaluated by MicroCT.
  
  
  RESULTS
  CBE prevented total BMD decrease in the femur induced by OVX, which was accompanied by a significant decrease in skeletal remodeling, as was evidenced by the decreased levels of the bone turnover markers, such as osteocalcin (OC), alkaline phosphatese (ALP), deoxypyridinoline (DPD), and urinary Ca and P excretions. The treatment could also enhance the bone strength and prevent the deterioration of trabecular microarchitecture.
  
  
  CONCLUSIONS
  The present study indicated that Cibotium barometz extract might be a potential alternative medicine for the prevention and treatment of postmenopausal osteoporosis.
authors.affiliation: Department of Orthopeadics, Xijing Hospital, The Fourth Military Medical University, No 17 Changlexi Road, Xi'an 710032, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhao
authors.forename: Zi-Xiang
authors.initials: ZX
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ya-Bo
authors.initials: YB
authors.lastname: Yan
authors.forename: Qiang
authors.initials: Q
authors.lastname: He
authors.forename: Peng-Chong
authors.initials: PC
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016723:Bone Remodeling / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D055119:Elastic Modulus; D004958:Estradiol / Q000494:pharmacology; D015914:Estrogen Replacement Therapy; D005260:Female; D005269:Femur / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000378:metabolism; D029624:Ferns / Q000737:chemistry; D010024:Osteoporosis / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D010052:Ovariectomy; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D050071:Bone Density Conservation Agents; D004365:Drugs, Chinese Herbal; D004958:Estradiol