title: [Molecular cloning and expression analysis of sucrose synthase gene from Dendrobium officinale].
abstract: OBJECTIVE
  Clone of sucrose synthase of Dendribium officinale and expression analysis, to provide the theory basis for research the relationship between polysaccharide synthesis of D. officinale and sucrose synthase activity.
  
  
  METHODS
  According to homologous sequence of sucrose synthase gene on GenBank, application the technology of RT-PCR and RACE, clone the full length of D. officinale. Target gene amplified with T vector was transformed into competent E. coli. BL21, IPTG induced expression, SDS-PAGE analysis.
  
  
  RESULTS
  A full length cDNA encoding sucrose synthase was isolated from the D. officinale, named DOSS1, the GenBank accession number is HQ856835, the cDNA is 2781 bp in length containing an open reading frame of 2424 bp encoding 807 amino acids with a predicted molecular mass of 92.3 x 10(3), the deduced amino acid sequence of D. officinale sucrose synthase shares 95% identity with Mokara yellow (AF530568); shares 90% identity with Oncidium goldiana (AF530567); shares more than 80% with other monocotyledonous plants.
  
  
  CONCLUSIONS
  Cloned the sucrose synthase gene and induced an obvious band successfully.
authors.affiliation: Institute of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China. menghengl@163.com
authors.forename: Hengling
authors.initials: H
authors.lastname: Meng
authors.forename: Chengli
authors.initials: C
authors.lastname: Duan
authors.forename: Fenghui
authors.initials: F
authors.lastname: Xiao
authors.forename: Shengchao
authors.initials: S
authors.lastname: Yang
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Zha
authors.forename: Guosong
authors.initials: G
authors.lastname: Wen
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D020121:5' Untranslated Regions / Q000235:genetics; D003001:Cloning, Molecular; D031667:Dendrobium / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011134:Polysaccharides / Q000096:biosynthesis
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D011134:Polysaccharides; D005964:Glucosyltransferases; C022699:sucrose synthase