title: Cellular response to the deposition of diesel exhaust particle aerosols onto human lung cells grown at the air-liquid interface by inertial impaction.
abstract: The pathogenesis of disease resulting from exposure to diesel exhaust particles (DEP) is often studied using cultured lung cells. Frequently, researchers expose cells to DEP by spiking a suspension of particles in liquid onto the apical surface. This is not representative of in vivo exposure, where aerosols are deposited onto cell surfaces at the air-liquid interface (ALI). Inertial impaction provides an opportunity to deliver high doses of particles with aerodynamic diameters>∼1 μm to the surface of cells in seconds in a reproducible and predictable manner. A custom device was constructed to deposit DEP aerosols onto the surface of Calu-3 and A549 cells grown at the ALI. The pro-inflammatory and toxic cellular response to exposure to the deposited DEP aerosols was measured and compared to the response of cells exposed to DEP as suspensions. Calu-3 cells showed evidence of an oxidative stress response for both exposure types, while there was strong evidence to suggest that the method of aerosol delivery was harmful to the A549 cells.
authors.affiliation: Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Cooney
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Hickey
mesh_terms: D000336:Aerosols; D000393:Air Pollutants / Q000633:toxicity*; D001273:Atmosphere Exposure Chambers; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004867:Equipment Design; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000166:cytology; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D018675:Toxicity Tests / Q000295:instrumentation* / Q000379:methods; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D000336:Aerosols; D000393:Air Pollutants; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D001335:Vehicle Emissions; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007770:L-Lactate Dehydrogenase; C022616:thiazolyl blue