title: [Effect of phospholipid composition on pharmaceutical properties and anti-tumor activity of stealth liposomes containing brucine].
abstract: OBJECTIVE
  To compare the pharmaceutical properties and the anti-tumor activities of three kinds of stealth liposomes prepared with different phospholipid composition containing brucine.
  
  
  METHODS
  Stealth liposomes with different phospholipids composition, such as soybean phosphatidycholine (SPC), hydrogenated soybean phosphatidylcholine (HSPC) and the complex of SPC and HSPC, were prepared by ammonium sulfate transmembrane gradient method. Pharmaceutical properties such as shape, encapsulation efficiency and size of three stealth liposomes were compared intensively. Anti-tumor activity of SPC, HSPC and novel stealth liposomes composed of both SPC and HSPC were compared by established mouse liver cancer H22 model. Meanwhile, the mice body weight and immune organ weight were also compared.
  
  
  RESULTS
  The encapsulation efficiency of novel, SPC and HSPC stealth liposomes were 77.7%, 64.8% and 74.8%, respectively. The mean diameters of them were less than 100 nm. The tumor inhibition rate of novel, HSPC and SPC stealth liposomes were 57.88%, 49.15%, 23.37%, respectively. The mice body weight, thymus gland index of three stealth liposomes group and spleen index of novel stealth liposomes group had no significant difference with the negative group while SPC and HSPC stealth liposomes group increased the spleen index.
  
  
  CONCLUSIONS
  Phospholipids composition is the key factor which determines the antitumor activity of brucine-loaded stealth liposomes.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. chenminglei_042005@163.com
authors.forename: Minglei
authors.initials: M
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Hou
authors.forename: Yun
authors.initials: Y
authors.lastname: Fang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Sun
authors.forename: Rongrong
authors.initials: R
authors.lastname: Hu
authors.forename: Baocang
authors.initials: B
authors.lastname: Cai
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000009:adverse effects / Q000737:chemistry*; D051379:Mice; D010316:Particle Size; D010743:Phospholipids / Q000737:chemistry*; D013331:Strychnine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D010743:Phospholipids; C083806:brucine; D013331:Strychnine