title: Laser microdissection for gene expression profiling.
abstract: Microarray-based gene expression profiling is revolutionizing biomedical research by allowing expression profiles of thousands of genes to be interrogated in a single experiment. In cancer research, the use of laser microdissection (LM) to isolate RNA from tissues provides the ability to accurately identify molecular profiles from different cell types that comprise the tumor and its surrounding microenvironment. Because RNA is an unstable molecule, the quality of RNA extracted from tissues can be affected by sample preparation and processing. Thus, special protocols have been developed to isolate research-quality RNA after LM. This chapter provides detailed descriptions of protocols used to generate micro-array data from high-quality frozen breast tissue specimens, as well as challenges associated with formalin-fixed paraffin-embedded specimens.
authors.affiliation: Windber Research Institute, Windber, PA, USA.
authors.forename: Lori A
authors.initials: LA
authors.lastname: Field
authors.forename: Brenda
authors.initials: B
authors.lastname: Deyarmin
authors.forename: Craig D
authors.initials: CD
authors.lastname: Shriver
authors.forename: Darrell L
authors.initials: DL
authors.lastname: Ellsworth
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Ellsworth
mesh_terms: D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008867:Microtomy / Q000379:methods; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016612:Paraffin Embedding; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D012313:RNA