title: Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression.
abstract: OBJECTIVE
  To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.
  
  
  METHODS
  Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. Then the expression and activity of matrix metalloproteinase (MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells.
  
  
  RESULTS
  18 nmol/L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol/L triptolide and 3 μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells, by about 50% and 35%, respectively, compared with the control. Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells.
  
  
  CONCLUSIONS
  These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha 410013, P.R. China.
authors.forename: Shengbo
authors.initials: S
authors.lastname: Yang
authors.forename: Can
authors.initials: C
authors.lastname: Gu
authors.forename: Guiying
authors.initials: G
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Haiquan
authors.initials: H
authors.lastname: Wen
authors.forename: Qianjin
authors.initials: Q
authors.lastname: Lu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Huang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000494:pharmacology*; D015536:Down-Regulation; D004852:Epoxy Compounds / Q000494:pharmacology; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D061965:Matrix Metalloproteinase Inhibitors; D009361:Neoplasm Invasiveness; D010616:Phenanthrenes / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D004852:Epoxy Compounds; D061965:Matrix Metalloproteinase Inhibitors; D010616:Phenanthrenes; C001899:triptolide; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9