title: Detection of the effect of environmental toxicants on the synthetic peptide modified neural cell chip.
abstract: A cell based chip was designed to differentiate and to detect the effects of environmental chemicals on the neurite outgrowth in PC12 cell. To fabricate platform of cell chip, gold surfaces were modified by RGD based synthetic oligopeptide. Nanoscale controlled self-assembled peptide layer was investigated by Atomic Force Microscopy (AFM). On the fabricated cell chip, PC12 cell was immobilized and the differentiation of neurite outgrowth in PC12 cells was done by neurite growth factor (NGF). Differentiation of PC12 cell was confirmed by immunofluorescence study. Further the differentiation and the length of neurite was confirmed by confocal microscopy study. Voltammetry behavior of the neurite induced PC12 and the electrochemical behavior of the environmental toxicants effect on the neurite outgrowth was measured by cyclic voltammetry (CV). Self-assembled layer mediated cell immobilization technique and voltammetric signal analysis system can be applied to construct the neural cell chip for the detection of large number of environmental toxins and various neurotoxicants.
authors.affiliation: Interdisciplinary Program of Integrated Biotechnology, Sogang University, #1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
authors.forename: Rama
authors.initials: R
authors.lastname: Bhusal
authors.forename: Cheol-Heon
authors.initials: CH
authors.lastname: Yea
authors.forename: Waleed Ahmed
authors.initials: WA
authors.lastname: El-Said
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D016716:PC12 Cells; D010455:Peptides / Q000737:chemistry*; D051381:Rats
chemical_list: D004785:Environmental Pollutants; D010455:Peptides