title: The role of regulatory T cells during the attenuation of graft-versus-leukemia activity following donor leukocyte infusion in mice.
abstract: We investigated how the graft-versus-leukemia (GVL) effect is attenuated in the tumor microenvironment using a murine model of non-myeloablative allo-HSCT (NM-HSCT) plus delayed donor leukocyte infusion (DLI) in a haploidentical B6→F1 strain combination. In-line with aggravated leukemia growth, the proportions of effector T cells expressing IFN-γ (Teffs) in spleen were reduced and attenuated GVL activity was found to be accompanied by a rebound in CD4(+)Foxp3(+) regulatory T cells (Tregs) in tumor-draining lymph nodes and tumor tissues. DLI-derived Tregs and Teffs may be potential indicators of presence of leukemic progression after DLI in this GVL model.
authors.affiliation: Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Choi
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lim
authors.forename: Byung-Sik
authors.initials: BS
authors.lastname: Cho
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Nack-Gyun
authors.initials: NG
authors.lastname: Chung
authors.forename: Dae Chul
authors.initials: DC
authors.lastname: Jeong
authors.forename: Hyewon
authors.initials: H
authors.lastname: Youn
authors.forename: Chulbom
authors.initials: C
authors.lastname: Lee
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Choi
authors.forename: Chang-Ki
authors.initials: CK
authors.lastname: Min
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology; D020544:Graft vs Leukemia Effect / Q000276:immunology* / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D007938:Leukemia / Q000276:immunology / Q000473:pathology / Q000628:therapy; D017708:Leukocyte Transfusion; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008954:Models, Biological; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D014019:Tissue Donors; D014184:Transplantation, Homologous
chemical_list: D049452:Green Fluorescent Proteins