title: Capillary electrophoresis time-of-flight mass spectrometry for a confident elucidation of a glycopeptide map of recombinant human erythropoietin.
abstract: Capillary electrophoresis coupled to orthogonal accelerated time-of-flight mass spectrometry (CE/TOFMS) was used for the analysis of O- and N-glycopeptides of recombinant human erythropoietin (rhEPO). O(126) and N(83) with a tetraantennary complex type glycan (N(83)-4Ant) were selected as glycopeptide models to develop an optimum CE/TOFMS methodology capable of detecting and characterizing the wide variety of glycopeptides present in the glycoprotein digest. Glycopeptide adsorption in the inner surface of the fused-silica capillary was prevented after using a capillary conditioning of 1 M HAc between runs. On the other hand, different acidic conditions in the sheath liquid (SL) and in the background electrolyte (BGE) were tested with the aim of studying their influence in glycopeptide fragmentation. Finally, the fragmentor voltage value of the TOF-MS instrument was optimized to avoid the involuntary fragmentation of the native glycopeptides. Hence, the established method may be regarded as an excellent starting point to obtain reliable glycopeptide maps of complex glycoproteins such as rhEPO by CE/TOFMS.
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Diagonal 647, 08028 Barcelona, Spain. estelagimenez@ub.edu
authors.forename: Estela
authors.initials: E
authors.lastname: Giménez
authors.forename: Raquel
authors.initials: R
authors.lastname: Ramos-Hernan
authors.forename: Fernando
authors.initials: F
authors.lastname: Benavente
authors.forename: José
authors.initials: J
authors.lastname: Barbosa
authors.forename: Victoria
authors.initials: V
authors.lastname: Sanz-Nebot
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004921:Erythropoietin / Q000737:chemistry*; D006020:Glycopeptides / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D011994:Recombinant Proteins
chemical_list: D006020:Glycopeptides; D010446:Peptide Fragments; D011994:Recombinant Proteins; D004921:Erythropoietin