title: Biomarkers of organophosphorus (OP) exposures in humans.
abstract: There are ongoing events where aircraft engine lubricant containing tricresyl phosphates (TCPs) contaminates aircraft cabins. Some individuals have experienced tremors or other neurological symptoms that may last for many months following exposures. Mass spectrometric (MS) protocols are being developed to determine the percentage of "biomarker proteins" that are modified by such exposures, specifically on active site serines. Both plasma butyrylcholinesterase (BChE) and red cell acylpeptide hydrolase (APH) are readily inhibited by 2-(ortho-cresyl)-4H-1,3,2-benzodioxaphosphoran-2-one (CBDP) or phenyl saligenin cyclic phosphate (PSP) and have the potential to provide information about the level of exposure of an individual. We have developed immunomagnetic bead-based single-step purification protocols for both BChE and APH and have characterized the active site serine adducts of BChE by MS.
authors.affiliation: Department of Medicine, Division Medical Genetics, University of Washington, Seattle, WA 98195-7720, United States.
authors.forename: Judit
authors.initials: J
authors.lastname: Marsillach
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Richter
authors.forename: Jerry H
authors.initials: JH
authors.lastname: Kim
authors.forename: Richard C
authors.initials: RC
authors.lastname: Stevens
authors.forename: Michael J
authors.initials: MJ
authors.lastname: MacCoss
authors.forename: Daniela
authors.initials: D
authors.lastname: Tomazela
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Suzuki
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Schopfer
authors.forename: Oksana
authors.initials: O
authors.lastname: Lockridge
authors.forename: Clement E
authors.initials: CE
authors.lastname: Furlong
mesh_terms: D015415:Biomarkers / Q000097:blood; D002091:Butyrylcholinesterase / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004781:Environmental Exposure / Q000009:adverse effects*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009943:Organophosphorus Compounds / Q000097:blood*; D010447:Peptide Hydrolases / Q000097:blood
chemical_list: D015415:Biomarkers; D009943:Organophosphorus Compounds; D002091:Butyrylcholinesterase; D010447:Peptide Hydrolases; C038220:acylaminoacyl-peptidase