title: Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior.
abstract: Bone regeneration can be accelerated by localized delivery of appropriate growth factors/biomolecules. Localized delivery can be achieved by a 2-level system: (i) incorporation of biomolecules within biodegradable particulate carriers (nanoparticles), and (ii) inclusion of such particulate carriers (nanoparticles) into suitable porous scaffolds. In this study, freeze-dried porous chitosan-gelatin scaffolds (CH-G: 1:2 ratio by weight) were embedded with various amounts of poly(lactide-co-glycolide) (PLGA) nanoparticles, precisely 16.6%, 33.3% and 66.6% (respect to CH-G weight). Scaffolds loaded with PLGA nanoparticles were subjected to physico-mechanical and biological characterizations including morphological analysis, swelling and dissolution tests, mechanical compression tests and cell viability tests. Results showed that incorporation of PLGA nanoparticles into porous crosslinked CH-G scaffolds: (i) changed the micro-architecture of the scaffolds in terms of mean pore diameter and pore size distribution, (ii) reduced the dissolution degree of the scaffolds, and (iii) increased the compressive modulus. On the other hand, the water uptake behavior of CH-G scaffolds containing PLGA nanoparticles significantly decreased. The incorporation of PLGA nanoparticles did not affect the biocompatibility of CH-G scaffolds.
authors.affiliation: Department of Mechanics, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Turin, Italy. vijay.polito@gmail.com
authors.forename: Vijay Kumar
authors.initials: VK
authors.lastname: Nandagiri
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Gentile
authors.forename: Valeria
authors.initials: V
authors.lastname: Chiono
authors.forename: Chiara
authors.initials: C
authors.lastname: Tonda-Turo
authors.forename: Amos
authors.initials: A
authors.lastname: Matsiko
authors.forename: Zeibun
authors.initials: Z
authors.lastname: Ramtoola
authors.forename: Franco Maria
authors.initials: FM
authors.lastname: Montevecchi
authors.forename: Gianluca
authors.initials: G
authors.lastname: Ciardelli
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D005780:Gelatin; D048271:Chitosan