title: Bioinformatics analysis and prediction for structure and function of nitric oxide synthase and similar proteins from Plasmodium berghei.
abstract: OBJECTIVE
  To search and analyze nitric oxide synthase (NOS) and similar proteins from Plasmodium berghei(Pb).
  
  
  METHODS
  The structure and function of nitric oxide synthase and similar proteins from Plasmodium berghei were analyzed and predicted by bioinformatics.
  
  
  RESULTS
  PbNOS were not available, but nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium (NADPH)-cytochrome p450 reductase(CPR) were gained. PbCPR was in the nucleus of Plasmodium berghei, while 134aa-229aa domain was localize in nucleolar organizer. The amino acids sequence of PbCPR had the closest genetic relationship with Plasmodium vivax showing a 73% homology. The tertiary structure of PbCPR displayed the forcep-shape with wings, but no wings existed in the tertiary structure of its' host, Mus musculus(Mm). 137aa-200aa, 201aa-218aa, 220aa-230aa, 232aa-248, 269aa-323aa, 478aa-501aa and 592aa-606aa domains of PbCPR showed no homology with MmCPRs', and all domains were exposed on the surface of the protein.
  
  
  CONCLUSIONS
  NOS can't be found in Plasmodium berghei and other Plasmodium species. PbCPR may be a possible resistance site of antimalarial drug, and the targets of antimalarial drug and vaccine. It may be also one of the mechanisms of immune evasion. This study on Plasmodium berghei may be more suitable to Plasmodium vivax. And 137aa-200aa, 201aa-218aa, 220aa-230aa, 232aa-248, 269aa-323aa, 478aa-501aa and 592aa-606aa domains of PbCPR are more ideal targets of antimalarial drug and vaccine.
authors.affiliation: School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571101, PR China. yuyanghoutu2006@yahoo.com.cn
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Fan
authors.forename: Gang
authors.initials: G
authors.lastname: Lv
authors.forename: Lingmin
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Gan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Saifeng
authors.initials: S
authors.lastname: Zhong
authors.forename: Guogang
authors.initials: G
authors.lastname: Yan
authors.forename: Guifen
authors.initials: G
authors.lastname: Lin
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology; D051379:Mice; D008958:Models, Molecular; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D010962:Plasmodium berghei / Q000201:enzymology* / Q000235:genetics*; D010966:Plasmodium vivax / Q000235:genetics; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D015800:Protozoan Proteins; D019001:Nitric Oxide Synthase; D009251:NADPH-Ferrihemoprotein Reductase