title: Actin filament segmentation using dynamic programming.
abstract: We introduce a novel algorithm for actin filament segmentation in 2D TIRFM image sequences. This problem is difficult because actin filaments dynamically change shapes during their growth, and the TIRFM images are usually noisy. We ask a user to specify the two tips of a filament of interest in the first frame. We then model the segmentation problem in an image sequence as a temporal chain, where its states are tip locations; given candidate tip locations, actin filaments' body points are inferred by a dynamic programming method, which adaptively generates candidate solutions. Combining candidate tip locations and their inferred body points, the temporal chain model is efficiently optimized using another dynamic programming method. Evaluation on noisy TIRFM image sequences demonstrates the accuracy and robustness of this approach.
authors.affiliation: Department of Computer Science & Engineering, Lehigh University, USA.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Li
authors.forename: Tian
authors.initials: T
authors.lastname: Shen
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Huang
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000465:Algorithms; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity