title: Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis.
abstract: Clover yellow vein virus (ClYVV) causes lethal systemic necrosis in legumes, including broad bean (Vicia faba) and pea (Pisum sativum). To identify host genes involved in necrotic symptom expression after ClYVV infection, we screened cDNA fragments in which expression was changed in advance of necrotic symptom expression in broad bean (V. faba cv. Wase) using the differential display technique and secondarily with Northern blot analysis. Expression changes were confirmed in 20 genes, and the six that exhibited the most change were analyzed further. These six genes included a gene that encodes a putative nitrate-induced NOI protein (VfNOI), and another was homologous to an Arabidopsis gene that encodes a glycine- and proline-rich protein GPRP (VfGPRP). We recently reported that necrotic symptom development in ClYVV-infected pea is associated with expression of salicylic acid (SA)-dependent pathogenesis-related (PR) proteins and requires SA-dependent host responses. Interestingly, VfNOI and VfGPRP expression was correlated with that of the putative SA-dependent PR proteins in ClYVV-infected broad bean. However, broad bean infected with a recombinant ClYVV expressing the VfGPRP protein showed weaker symptoms and less viral multiplication than that infected with ClYVV expressing the GFP protein. These results imply that VfGPRP plays a role in defense against ClYVV rather than in necrotic symptom expression.
authors.affiliation: Pathogen-Plant Interactions Group, Plant Breeding Science, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. knakahar@res.agr.hokudai.ac.jp
authors.forename: Kenji S
authors.initials: KS
authors.lastname: Nakahara
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kitazawa
authors.forename: Go
authors.initials: G
authors.lastname: Atsumi
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Choi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Uyeda
mesh_terms: D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018076:DNA, Complementary / Q000032:analysis / Q000096:biosynthesis; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009336:Necrosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D018532:Pisum sativum / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000821:virology; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D057865:Plant Immunity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017800:Potyvirus / Q000472:pathogenicity / Q000502:physiology*; D020156:Salicylic Acid / Q000378:metabolism; D016415:Sequence Alignment; D031307:Vicia faba / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D020156:Salicylic Acid