title: A 2-D gel reference map of the basic human heart proteome.
abstract: We have undertaken the identification of basic proteins (pH 6-11) of the human heart using 2-DE. Tissue from the left ventricle of human heart was lysed and proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading followed by separation on 12% SDS polyacrylamide gels in the second dimension. Proteins were then identified by mass spectrometry and analysed using Proline, a proteomic data analysis platform that was developed in-house. The proteome map contains 176 identified spots with 151 unique proteins and has been made available as part of the UCD-2DPAGE database at http://proteomics-portal.ucd.ie:8082. The associated mass spectrometry data have been submitted to PRIDE (Accession number ♯10098). This reference map, and the other heart reference maps available through the UCD-2DPAGE database, will aid further proteomic studies of heart diseases such as dilated cardiomyopathy and ischaemic heart disease.
authors.affiliation: UCD Conway Institute of Biomolecular and Biomedical Research, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. julie.polden@ucd.ie
authors.forename: Julie
authors.initials: J
authors.lastname: Polden
authors.forename: Ciara A
authors.initials: CA
authors.lastname: McManus
authors.forename: Cris
authors.initials: C
authors.lastname: Dos Remedios
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dunn
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*
chemical_list: D020543:Proteome