title: [SIRT1 inhibits IL-1β mRNA transcription in lipopolysaccharide tolerant THP-1 cells].
abstract: OBJECTIVE
  To explore the role of silent information regulation 2 homolog 1 (SIRT1) in the regulation of IL-1β mRNA transcription in lipopolysaccharide (LPS) tolerant THP-1 cells.
  
  
  METHODS
  THP-1 human promonocyte model of endotoxin tolerance that simulates the sepsis leukocyte phenotype was used. Chromatin immunoprecipitation assay (ChIP) and real-time PCR were applied to quantify the binding of SIRT1 and histone H3 lys9/H4 lys16 acetylation to IL-1β promoter. IL-1β mRNA transcription was studied after knocking down the SIRT1.
  
  
  RESULTS
  The binding of SIRT1 to IL-1β promoter increased about 5 times in tolerant THP-1 cells (P < 0.05), which was accompanied by the low level of histone H3 lys9/H4 lys16 acetylation (P < 0.05, compared with normal cells). Knocking-down of SIRT1 increased the transcription of IL-1β mRNA up to the level of 68% of normal cells (P < 0.05), which was accompanied by the increase of histone H3 lys9/H4 lys16 acetylation (P < 0.05). However, there was no significant difference of p65 lys310 acetylation between normal and tolerant cells.
  
  
  CONCLUSIONS
  SIRT1 inhibited the IL-1β mRNA transcription in tolerant THP-1 cells but had not related to p65 lys310 acetylation. However, it was related to IL-1β promoter acetylation.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Chen
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Li
authors.forename: Mei-li
authors.initials: ML
authors.lastname: Cong
authors.forename: Yan-jun
authors.initials: YJ
authors.lastname: Kang
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Guo
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000107:Acetylation; D002460:Cell Line; D006657:Histones / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008070:Lipopolysaccharides; D012333:RNA, Messenger / Q000235:genetics; D056564:Sirtuin 1 / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D006657:Histones; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D012333:RNA, Messenger; C447939:SIRT1 protein, human; D056564:Sirtuin 1