title: Bio-electrosprayed living composite matrix implanted into mouse models.
abstract: We show that composite de novo structures can be generated using bio-electrosprays. Mouse lung fibroblasts are bio-electrosprayed directly with a biopolymer to form cell-bearing matrices, which are viable even when implanted subcutaneously into murine hosts. Generated cell-bearing matrices are assessed in-vitro and found to undergo all expected cellular behaviour. Subsequent in-vivo studies demonstrate the implanted living matrices integrating as expected with the surrounding microenvironment. The in-vitro and in-vivo studies elucidate and validate the ability for either bio-electrosprays or cell electrospinning to form a desired living architecture for undergoing investigation for repairing, replacing and rejuvenating damaged and/or ageing tissues.
authors.affiliation: BioPhysics Group, Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom. s.jayasinghe@ucl.ac.uk
authors.forename: Suwan N
authors.initials: SN
authors.lastname: Jayasinghe
authors.forename: Gary
authors.initials: G
authors.lastname: Warnes
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Scotton
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005434:Flow Cytometry; D008168:Lung / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D010641:Phenotype; D011419:Propidium / Q000378:metabolism; D019736:Prostheses and Implants; D040521:Subcutaneous Tissue / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017304:Annexin A5; D001672:Biocompatible Materials; D011419:Propidium