title: [Identification of protein markers for gestational diabetes mellitus complicated by pregnancy-induced hypertensive syndrome].
abstract: OBJECTIVE
  To identify the serum protein markers for the gestational diabetes mellitus (GDM) complicated by pregnancy-induced hypertensive (PIH) syndrome to provide a molecular biological basis for the screening, prevention and therapy of the related diseases.
  
  
  METHODS
  Serum samples were collected from the patients with GDM, PIH syndrome, and GDM complicated by PIH syndrome. IgG and albumins were removed from the samples before SDS -PAGE. The protein bands showing significant differences among the 3 samples were collected, digested and identified with mass spectrometry, and the function of the identified proteins was analyzed.
  
  
  RESULTS
  Three SDS-PAGE were performed in parallel to confirm the differentially expressed proteins. Mass spectrometry indicated that the proteins showing obvious differences among the 3 samples were haptoglobin, protein SMG8 and apoptosis-inducing factor-1.
  
  
  CONCLUSIONS
  The protein markers identified in GDM complicated by PIH syndrome may be integrated into the proteomic database of gestational metabolic diseases. Identification of the associated protein markers may provide significant experimental data for the prevention, diagnosis and therapy of the related diseases.
authors.affiliation: Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. wangshuoshi1985@126.com
authors.forename: Shuo-shi
authors.initials: SS
authors.lastname: Wang
authors.forename: Shui-wang
authors.initials: SW
authors.lastname: Hu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
mesh_terms: D000328:Adult; D051033:Apoptosis Inducing Factor / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016640:Diabetes, Gestational / Q000097:blood* / Q000175:diagnosis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006242:Haptoglobins / Q000032:analysis*; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood* / Q000175:diagnosis; D011247:Pregnancy; D040901:Proteomics / Q000379:methods; D055815:Young Adult
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D015415:Biomarkers; D006242:Haptoglobins