title: Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer.
abstract: This chapter refers to the application of laser-capture microdissection with oligonucleotide microarray analysis. The protocol described has been successfully used to identify differential transcript expression between contrasting colorectal cancer invasive phenotypes. Tissue processing, RNA extraction, quality control, amplification, fluorescent labelling, purification, hybridisation, and elements of data analysis are covered.
authors.affiliation: Department of Academic Surgery, St. James's University Hospital, Leeds, UK. christhorn77@yahoo.com
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Thorn
authors.forename: Deborah
authors.initials: D
authors.lastname: Williams
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Freeman
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D003627:Data Interpretation, Statistical; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012984:Software; D013194:Staining and Labeling / Q000379:methods
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger