title: [The relationship between phosphatidylinositol-3-kinases/protein-serine-threonine kinase signaling pathway and orthodontic tooth movement].
abstract: OBJECTIVE
  To study the relationship between phosphatidylinositol-3-kinases(PI3K)/protein-serine-threonine kinase(AKt) signaling pathway and orthodontic tooth movement.
  
  
  METHODS
  Twenty-four rabbits were chosen to establish rabbit models for the study. The right maxillary teeth of each animal treated by orthodontics were as the test side, and the untreated left teeth were as the control side. The animals were sacrificed at 3, 5, 7, 14 d, respectively. The prepared tissue specimens were processed for the study. The changes of the expression of PI3K, AKt in periodontal tissues were detected by real-time quantitative-polymerase chain reaction (RQ-PCR) and Western blot techniques.
  
  
  RESULTS
  RQ-PCR showed that the expression of PI3K, AKt mRNA dramatically changed at 3 d. The expression of PI3K, AKt mRNA in the test side was higher than the control side, especially at 7 d, and then decreased. Compared with the control side, there was statistical significant difference in the test side(P < 0.05). The study obtained consistent conclusion from Western blot and RQ-PCR.
  
  
  CONCLUSIONS
  Expression of PI3K, AKt in rabbit periodontal tissues increase during orthodontic tooth movement, which prompts that PI3K/AKt signal pathways relate to orthodontic tooth movement and PI3K/AKt signal pathway involve in the periodontal tissue remodeling.
authors.affiliation: Fengtian Dental Clinic, School of Stomatology, China Medical University, Shenyang 110013, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Su-Fen
authors.initials: SF
authors.lastname: Sun
mesh_terms: D000818:Animals; D019869:Phosphatidylinositol 3-Kinases; D010716:Phosphatidylinositols; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; D011817:Rabbits; D015398:Signal Transduction; D014087:Tooth Movement Techniques
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D010716:Phosphatidylinositols; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger