title: Effects of dietary oxidized oil on laying performance, lipid metabolism, and apolipoprotein gene expression in laying hens.
abstract: We studied the effects of dietary oxidized oils on serum lipid metabolic indices, estradiol level, and the gene expression of apolipoprotein B-100 and apolipoprotein VLDL-II in laying hens. Hy-Line Grey hens (280 ± 10 d old; average egg production, 90.0 ± 2.5%) were allotted to 1 of 4 dietary treatments, which were supplemented with 0 (control group), 1% (low oxidized group), 2% (moderately oxidized group), or 4% (highly oxidized group) thermally oxidized soybean oil. Each treatment contained 6 replicates, with 12 birds each. The feeding trial lasted for 30 d. Laying performance data were recorded weekly. Other indices were measured on d 0, 2, 6, 14, and 30 of the feeding trial. Hens in the moderately and highly oxidized groups had significantly lowered feed conversion ratios (P < 0.05). Those in the highly oxidized group also had decreased concentrations of serum very low density lipoprotein cholesterol on d 30 (P < 0.05) compared with the very low density lipoprotein cholesterol of hens in the moderately oxidized group. Hens in the moderately oxidized group had significantly increased expression of apolipoprotein B-100 (P < 0.05) from d 6 to 30. Consequently, hepatic triglyceride increased in this group on d 30 (P < 0.05). Serum triglyceride decreased in the moderately oxidized group on d 30 (P < 0.05), which may have been caused by the activation of peroxisome proliferator-activating receptor α. Serum estradiol levels were not significantly affected by oxidized oils at any time of measurement, but were significantly different between d 0 and 30 within the moderately oxidized group. This fact indicated that the effect of oxidized oils on apolipoprotein B-100 might partially be a cumulative result of the increasing secretion of estradiol. The results suggested that oxidized oil may affect the performance of laying hens through the regulation of apolipoproteins and estradiol.
authors.affiliation: Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Yue
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Qi
authors.forename: F
authors.initials: F
authors.lastname: Ji
authors.forename: M F
authors.initials: MF
authors.lastname: Liu
authors.forename: S G
authors.initials: SG
authors.lastname: Wu
authors.forename: H J
authors.initials: HJ
authors.lastname: Zhang
authors.forename: G H
authors.initials: GH
authors.lastname: Qi
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001053:Apolipoproteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens / Q000254:growth & development; D004032:Diet / Q000662:veterinary; D004042:Dietary Fats, Unsaturated / Q000032:analysis / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D001053:Apolipoproteins; D004042:Dietary Fats, Unsaturated; D008055:Lipids; D004958:Estradiol