title: Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association.
abstract: The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indicated that the maize lipoxygenase-1 was localized in cytoplasm. Sucrose-density fractionation experiment and in vitro protein transport to chloroplast showed that the maize lipoxygenase-1 can be associated with chloroplast. Secondary structure alignment revealed putative calcium binding sites in the PLAT domain of maize lipoxygenase-1 and the association of the maize lipoxygenase-1 with membranes was mediated by calcium ion in vitro. Our results provide evidences for calcium-mediated translocation of dual positional LOX without chloroplast targeting sequence from cytoplasm to chloroplast in plants for the first time.
authors.affiliation: Department of Molecular Biotechnology and Kumho Life Science Laboratory, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Kyoungwon
authors.initials: K
authors.lastname: Cho
authors.forename: Younsoo
authors.initials: Y
authors.lastname: Han
authors.forename: Je Chang
authors.initials: JC
authors.lastname: Woo
authors.forename: Bianca
authors.initials: B
authors.lastname: Baudisch
authors.forename: Ralf Bernd
authors.initials: RB
authors.lastname: Klösgen
authors.forename: Seunghan
authors.initials: S
authors.lastname: Oh
authors.forename: Jihoon
authors.initials: J
authors.lastname: Han
authors.forename: Oksoo
authors.initials: O
authors.lastname: Han
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins; D002736:Chloroplasts / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008084:Lipoxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D003313:Zea mays / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008084:Lipoxygenase; D002118:Calcium