title: Phosphoribosylamidotransferase, the first enzyme for purine de novo synthesis, is required for conidiation in the sclerotial mycoparasite Coniothyrium minitans.
abstract: Coniothyrium minitans is an important sclerotial parasite of the fungal phytopathogen, Sclerotinia sclerotiorum. Previously, we constructed a T-DNA insertional library, and screened for many conidiation-deficient mutants from this library. Here, we report a T-DNA insertional mutant ZS-1T21882 that completely lost conidiation. In mutant ZS-1T21882, the T-DNA was integrated into a gene (CmPrat-1) which encodes phosphoribosylamidotransferase (PRAT, EC 2.4.2.14), an enzyme catalyzing the first committed step in de novo purine nucleotide synthesis. Gene replacement and complementation experiments confirmed that phosphoribosylamidotransferase is essential for conidiation of C. minitans. Mutant ZS-1T21882 did not grow on modified Czapek-Dox broth (MCD), but it grew well on MCD amended with IMP or AMP. The conidial production of this mutant was dependent on the dosage of IMP amended. At low concentrations, such as 0.1 mM and 0.25 mM, the mutant produced very few pycnidia, while up to 0.75 mM or higher, the conidiation of this mutant was restored completely. cAMP could not restore the conidiation of mutant ZS-1T21882 when amended into MCD, but could when amended into PDA. Neither GMP nor cGMP could restore the conidiation in MCD or in PDA. Our findings suggest that phosphoribosylamidotransferase is essential for conidiation of C. minitans via adenosine related molecules. Furthermore, when dual cultured with its host, this mutant produced conidia in the host mycelium and on the sclerotia of S. sclerotiorum, but not in dead mycelium or on dead sclerotia, suggesting that C. minitans is likely to able to obtain adenosine or related components from its host during parasitization.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Gong
authors.forename: Jiatao
authors.initials: J
authors.lastname: Xie
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
authors.forename: Jiasen
authors.initials: J
authors.lastname: Cheng
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
authors.forename: Junbin
authors.initials: J
authors.lastname: Huang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fu
mesh_terms: D000241:Adenosine / Q000096:biosynthesis*; D000582:Amidophosphoribosyltransferase / Q000096:biosynthesis / Q000378:metabolism*; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000502:physiology; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics / Q000502:physiology; D025282:Mycelium / Q000201:enzymology; D015398:Signal Transduction; D013172:Spores, Fungal / Q000201:enzymology* / Q000235:genetics / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D000242:Cyclic AMP; D000582:Amidophosphoribosyltransferase; D000241:Adenosine