title: Immunoguided microdissection techniques.
abstract: Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain-based, automated approach becomes essential. In this chapter, we discuss the use of immunohistochemistry (IHC) and immunofluorescence (IF) to guide the microdissection process via manual and software-driven auto-dissection methods. Although technical challenges still exist with these innovative approaches, we present here methods and protocols to successfully perform immuno-based microdissection on commercially available laser dissection systems.
authors.affiliation: Pathogenetics Unit and Laser Microdissection Core, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Tangrea
authors.forename: Jeffrey C
authors.initials: JC
authors.lastname: Hanson
authors.forename: Robert F
authors.initials: RF
authors.lastname: Bonner
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Pohida
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodriguez-Canales
authors.forename: Michael R
authors.initials: MR
authors.lastname: Emmert-Buck
mesh_terms: D002469:Cell Separation / Q000379:methods; D015925:Cryopreservation; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods*; D007633:Keratins / Q000378:metabolism; D007834:Lasers; D008297:Male; D042282:Microdissection / Q000379:methods*; D016612:Paraffin Embedding; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D012984:Software; D016707:Tissue Fixation
chemical_list: D007633:Keratins