title: Minimizing matrix effects while preserving throughput in LC-MS/MS bioanalysis.
abstract: BACKGROUND
  Phospholipids are known to cause matrix effects in LC-MS analysis and are not effectively removed by one of the most common method of sample preparation: organic solvent protein precipitation. The objective of this research is to minimize phospholipid interferences chromatographically.
  
  
  RESULTS
  In this article we examine several chromatographic approaches and highlight the method we developed that allows for the rapid gradient separation of model drug molecules from phospholipids.
  
  
  CONCLUSIONS
  The new approach (which utilizes a mixture of methanol and acetonitrile as the organic mobile phase on a 2.1 × 20 mm C18 column) minimized phospholipids-related matrix effects in the analysis of plasma samples prepared by protein precipitation and is suitable for high-throughput bioanalysis in drug discovery.
authors.affiliation: Drug Innovation Pharmacokinetics, Vertex Pharmaceuticals Inc., Cambridge, MA 02139, USA.
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Tsao
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Brummel
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D010743:Phospholipids / Q000097:blood* / Q000737:chemistry*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000097:Acetonitriles; D004364:Pharmaceutical Preparations; D010743:Phospholipids; D000432:Methanol; C032159:acetonitrile