title: Targeted gene expression profiling in European sea bass (Dicentrarchus labrax, L.) follicles from primary growth to late vitellogenesis.
abstract: A real-time PCR-based gene expression survey was performed on isolated European sea bass follicles from primary growth to late vitellogenesis. Expression levels of 18 transcripts with demonstrated relevance during oogenesis, encoding gonadotropin, thyrotropin, estrogen, androgen, and vitellogenin receptors, steroidogenesis-related as well as growth and transcription factors were measured. Primary oocytes showed high mRNA levels of insulin-like growth factors 1 and 2, bone morphogenetic protein 4, estrogen receptor 2b, androgen receptor b, and SRY-box containing gene 17 together with low transcript amounts of gonadotropin receptors. Follicles at the lipid vesicles stage (i.e., the beginning of the secondary growth phase) showed elevated mRNA amounts of follicle stimulating hormone receptor (fshr) and anti-Mullerian hormone. Early-to-mid vitellogenic follicles showed high mRNA levels of fshr and cytochrome P450, family 19, subfamily A, polypeptide 1a while mid-to-late vitellogenic follicles expressed increasing transcript amounts of luteinizing hormone/choriogonadotropin receptor, steroidogenic acute regulatory protein, and estrogen receptors 1 and 2a. The molecular data presented here may serve as a solid base for future studies focused on unraveling the specific mechanisms orchestrating follicular development in teleost fish.
authors.affiliation: Departamento de Biología Marina y Acuicultura, Instituto de Ciencias Marinas de Andalucía (Consejo Superior de Investigaciones Científicas), Puerto Real, Cádiz, Spain. angel.garcia@icman.csic.es
authors.forename: Angel
authors.initials: A
authors.lastname: García-López
authors.forename: María Isabel
authors.initials: MI
authors.lastname: Sánchez-Amaya
authors.forename: Francisco
authors.initials: F
authors.lastname: Prat
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005060:Europe; D005260:Female; D020869:Gene Expression Profiling; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D006080:Ovarian Follicle / Q000254:growth & development; D017978:Receptors, Growth Factor / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014818:Vitellogenesis / Q000235:genetics*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D017978:Receptors, Growth Factor; D014157:Transcription Factors