title: Biochemical and histopathological investigation of the protective effect of disulfiram in ischemia-induced ovary damage.
abstract: It was biochemically and histopathologically investigated whether disulfiram has protective effects on ischemia-induced ovary damage. For this purpose, levels of tGSH, superoxide dismutase (SOD), malondialdehyde (MDA), and 8-OH Gua/Gua were investigated in ischemic rat ovary tissue. Results show that used doses of disulfiram (10, 25, and 50 mg/kg) prevent MDA, a product of ischemia-induced lipid peroxidation, formation in female rat ovary tissue and prevent decrease of enzymatic and non-enzymatic (SOD, GSH) antioxidant parameters. Additionally, all doses of disulfiram significantly prevent DNA damage when compared to control group. Fewer histopathological findings were observed in tissues with higher antioxidant levels and lower oxidant and DNA damage levels.
authors.affiliation: Nenehatun Obstetrics and Gynecology Hospital, Erzurum, Turkey.
authors.forename: Unal
authors.initials: U
authors.lastname: Isaoglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Muhammet
authors.initials: M
authors.lastname: Calik
authors.forename: Beyzagul
authors.initials: B
authors.lastname: Polat
authors.forename: Ebubekir
authors.initials: E
authors.lastname: Bakan
authors.forename: Ali
authors.initials: A
authors.lastname: Kurt
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Albayrak
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D004249:DNA Damage; D004221:Disulfiram / Q000627:therapeutic use*; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D005260:Female; D007511:Ischemia / Q000150:complications*; D010049:Ovarian Diseases / Q000209:etiology / Q000517:prevention & control*; D010053:Ovary / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology; D020011:Protective Agents / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004791:Enzyme Inhibitors; D020011:Protective Agents; D004221:Disulfiram