title: Novel SNPs in the PRDM16 gene and their associations with performance traits in chickens.
abstract: The PR domain containing 16 (PRDM16) is a member of the Prdm family, and is known to regulate cell differentiation. In the present study, DNA pool sequencing methods were employed to screen genetic variations in the chicken PRDM16 gene. The results revealed four novel single nucleotide polymorphisms (SNPs): NC_006108.2: g.92188G>A, XM_417551: c.1161C>T (Ala/Ala, 387aa), c.1233C>T (Ser/Ser, 411aa) and c.1433G>A (Ser/Asn, 478aa). The BglI polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect c.1161C>T, while HhaI Forced PCR-RFLP methods were used to detect 1233C>T and c.1433G>A in 964 chickens. The chickens comprised 38 grandparents, 66 F(1) parents and 860 F(2) birds derived from an F(2) resource population of Gushi chickens crossed with Anka broilers. The associations of the polymorphisms in the chicken PRDM16 gene with performance traits were analyzed in the 860 F(2) chickens. The results indicated that the three SNPs were significantly associated with growth, fatness and meat quality traits in the chickens. In particular, the polymorphisms of the missense SNP (c.1433G>A) had positive effects on chicken body weight and body size at different stages. It affected also fatness traits significantly. Comparison of the different genotypes of c.1433G>A showed that the GG genotype favored chicken growth and fatness traits.
authors.affiliation: College of Livestock Husbandry and Veterinary Engineering, Henan Research Center of Breeding Resources for Poultry, Henan Agricultural University, No 95 Wenhua Road, Zhengzhou, 450002 Henan, People's Republic of China. rlhan@126.com
authors.forename: Ruili
authors.initials: R
authors.lastname: Han
authors.forename: Yang
authors.initials: Y
authors.lastname: Wei
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Kang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Guirong
authors.initials: G
authors.lastname: Sun
authors.forename: Guoxi
authors.initials: G
authors.lastname: Li
authors.forename: Yichun
authors.initials: Y
authors.lastname: Bai
authors.forename: Yadong
authors.initials: Y
authors.lastname: Tian
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000367:Age Factors; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D049628:Body Size; D002645:Chickens / Q000235:genetics* / Q000254:growth & development*; D003433:Crosses, Genetic; D056726:Genetic Association Studies; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D008460:Meat / Q000592:standards*; D020125:Mutation, Missense / Q000235:genetics; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D014157:Transcription Factors