title: [Construction and expression of hTNF-alpha fusion protein mediated by MMP1].
abstract: This paper is aimed to present a research on fusion protein of human tumor necrosis factor-alpha (hTNF-alpha), matrix metalloproteinase 1 (MMP1), and foldon sequence using the methord of gene engineering. We transformed the recombinant plasmid, which contains the DNA sequences of hTNF-alpha, MMP1, and foldon sequence, into Rosetta2, and successfully induced the fusion protein to express under given conditions by isopropyl beta-D-1-Thiogalactopyranoside (IPTG). Then we purified the expression product through a glutathione S-transferase (GST) resin and collected the interested protein. This research may lay the groundwork for scientific research and clinical application.
authors.affiliation: Department of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China.
authors.forename: Qiaojiajie
authors.initials: Q
authors.lastname: Zhao
authors.forename: Gan
authors.initials: G
authors.lastname: Hou
authors.forename: Dinan
authors.initials: D
authors.lastname: Huang
authors.forename: Shuyong
authors.initials: S
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D020781:Matrix Metalloproteinase 1