title: One-pot synthesis and characterization of cross-linked quaternized chitosan microspheres as protein adsorbent.
abstract: The one-pot synthesis and characterization of cross-linked quaternized chitosan microspheres (CQCM) as a protein adsorbent are presented. First of all, chitosan particles were prepared by spray drying method, and then they were quaternized and cross-linked in turn with glycidyltrimethylammonium (GTMAC) chloride and glutaraldehyde in isopropanol containing 10% water in one-pot. The effect of the reaction temperature, reaction time and the amounts of added GTMAC and glutaraldehyde on the protein adsorption ability of CQCM was investigated. The adsorption behavior of the CQCM prepared in the optimum synthetic conditions was well described by the Langmuir isotherm with maximum adsorption capacity equal to 1424 mg BSA/g dry weight. The particle size ranged from 7.6 to 48.9 μm. The mechanism of adsorption-desorption of BSA to the CQCM was ion-exchange. Finally, the extraction of soybean peroxidase from crude soybean peroxidase solution using the CQCM was performed.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China.
authors.forename: Weican
authors.initials: W
authors.lastname: Zhang
authors.forename: Caiyun
authors.initials: C
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xuemei
authors.initials: X
authors.lastname: Lu
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002625:Chemistry, Organic / Q000379:methods*; D048271:Chitosan / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D008863:Microspheres; D010316:Particle Size; D009195:Peroxidase / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013025:Glycine max / Q000201:enzymology; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors
chemical_list: D003432:Cross-Linking Reagents; D004852:Epoxy Compounds; D000644:Quaternary Ammonium Compounds; D012710:Serum Albumin, Bovine; C045006:glycidyl trimethylammonium; D048271:Chitosan; D009195:Peroxidase; D005976:Glutaral