title: Differential global gene expression in cystic fibrosis nasal and bronchial epithelium.
abstract: Respiratory epithelium is the target of therapies, such as gene therapy, for cystic fibrosis (CF) lung disease. To determine the usefulness of the nasal epithelium as a pre-screen for lung-directed therapies, we profiled gene expression in CF and non-CF nasal and bronchial epithelium samples using Illumina HumanRef-8 Expression BeadChips. 863 genes were differentially expressed between CF and non-CF bronchial epithelium but only 15 were differentially expressed between CF and non-CF nasal epithelium (≥1.5-fold, P≤0.05). The most enriched pathway in CF bronchial epithelium was inflammatory response, whereas in CF nasal epithelium it was amino acid metabolism. We also compared nasal and bronchial epithelium in each group and identified differential expression of cellular movement genes in CF patients and cellular growth genes in non-CF subjects. We conclude that CF and non-CF nasal and bronchial epithelium are transcriptionally distinct and CF nasal epithelium is not a good surrogate for the lung respiratory epithelium.
authors.affiliation: Medical Genetics Section, Molecular Medicine Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, UK. v.c.ogilvie@ed.ac.uk
authors.forename: Varrie
authors.initials: V
authors.lastname: Ogilvie
authors.forename: Margaret
authors.initials: M
authors.lastname: Passmore
authors.forename: Laura
authors.initials: L
authors.lastname: Hyndman
authors.forename: Lisa
authors.initials: L
authors.lastname: Jones
authors.forename: Barbara
authors.initials: B
authors.lastname: Stevenson
authors.forename: Abigail
authors.initials: A
authors.lastname: Wilson
authors.forename: Heather
authors.initials: H
authors.lastname: Davidson
authors.forename: Robert R
authors.initials: RR
authors.lastname: Kitchen
authors.forename: Robert D
authors.initials: RD
authors.lastname: Gray
authors.forename: Pallav
authors.initials: P
authors.lastname: Shah
authors.forename: Eric W
authors.initials: EW
authors.lastname: Alton
authors.forename: Jane C
authors.initials: JC
authors.lastname: Davies
authors.forename: David J
authors.initials: DJ
authors.lastname: Porteous
authors.forename: A Christopher
authors.initials: AC
authors.lastname: Boyd
mesh_terms: D000293:Adolescent; D000328:Adult; D001980:Bronchi / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D007633:Keratins / Q000378:metabolism; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D055815:Young Adult
chemical_list: C505032:CFTR protein, human; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D007633:Keratins