title: Proteomic changes related to "bewildered" circulating platelets in the acute coronary syndrome.
abstract: Acute coronary syndromes (ACS) are associated with platelet activation. The aim of the present study was to study the protein expression level associated with glycolysis, oxidative stress, cytoskeleton and cell survival in platelets obtained during an ACS. Platelets from 42 coronary ischemic patients, divided into patients admitted within 24 h after the onset of chest pain (ACS group; n=16) and patients with stable coronary ischemic disease (CAD, n=26), were analyzed using proteomics. The expression levels of proteins involved in cellular cytoskeleton (F-actin capping, β-tubulin, α-tubulin isotypes 1 and 2, vinculin, vimentin and two Ras-related protein Rab-7b isotypes), glycolysis pathway (glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and two pyruvate kinase isotypes) and cellular-related antioxidant system (manganese superoxide dismutase) and even the expression and activity of glutathione-S-transferase were significantly reduced in platelets from ACS patients compared to CAD patients. Moreover, reduction in the expression of proteins associated with cell survival such as proteasome subunit β type 1 was also observed in ACS platelets compared with CAD platelets. Principal component and logistic regression analysis suggested the existence of factors (proteins) expressed in the platelets inversely associated with acute coronary ischemia. In summary, these results suggest the existence of circulating antioxidant, cytoskeleton and glycolytic-"bewildered" platelets during the acute phase of a coronary event.
authors.affiliation: Cardiovascular Research Unit, Cardiology Department, Hospital Clínico San Carlos, Madrid, Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: López-Farré
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Zamorano-Leon
authors.forename: Luis
authors.initials: L
authors.lastname: Azcona
authors.forename: Javier
authors.initials: J
authors.lastname: Modrego
authors.forename: Petra J
authors.initials: PJ
authors.lastname: Mateos-Cáceres
authors.forename: Juan
authors.initials: J
authors.lastname: González-Armengol
authors.forename: Pedro
authors.initials: P
authors.lastname: Villarroel
authors.forename: Rosario
authors.initials: R
authors.lastname: Moreno-Herrero
authors.forename: Pablo
authors.initials: P
authors.lastname: Rodríguez-Sierra
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura
authors.forename: Juan
authors.initials: J
authors.lastname: Tamargo
authors.forename: Carlos
authors.initials: C
authors.lastname: Macaya
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000378:metabolism*; D000368:Aged; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005434:Flow Cytometry; D006019:Glycolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D015539:Platelet Activation; D025341:Principal Component Analysis; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D020543:Proteome