title: Interleukin-6 up-regulates the expression of interleukin-15 is associated with MAPKs and PI3-K signaling pathways in the human keratinocyte cell line, HaCaT.
abstract: Interleukin (IL)-15 is an important inflammatory cytokine and plays a key role in autoimmune disease. At present, IL-15 gene expression and regulation related to many innate immunity trigger signals have been clarified in some specific cell types, but the relationship of IL-6 and IL-15 in the human keratinocyte cell line (HaCaT) is unknown. In this study, we investigated the effect of IL-6 on the expression of IL-15 and selected signaling pathways in HaCaT cells. Results demonstrated that IL-6 up-regulated the expression of IL-15 both at the mRNA and protein levels. Meanwhile, IL-6 was able to activate MAPKs-ERK1/2 and PI3K-AKT signaling pathways. Furthermore, the high expression of IL-15 induced by IL-6 was down-regulated while MAPKs-ERK1/2 and PI3K-AKT signaling pathways were, respectively, blocked by PD98059 and LY294002. These findings indicate that the expression of IL-15 up-regulated by IL-6 is associated with MAPKs-ERK1/2 and PI3K-AKT signaling pathways in HaCaT cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Anhui Medical University, 69 Meishan Road, Hefei, 230032, Anhui, China.
authors.forename: Qian-Shan
authors.initials: QS
authors.lastname: Tao
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chai
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jia
authors.forename: Sheng-Quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D002460:Cell Line; D002867:Chromones / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D019409:Interleukin-15 / Q000096:biosynthesis / Q000235:genetics*; D015850:Interleukin-6 / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
chemical_list: D002867:Chromones; D005419:Flavonoids; C569274:IL15 protein, human; C508600:IL6 protein, human; D019409:Interleukin-15; D015850:Interleukin-6; D009025:Morpholines; D012333:RNA, Messenger; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one