title: Application of real-time PCR stool assay for Helicobacter pylori detection and clarithromycin susceptibility testing in Brazilian children.
abstract: BACKGROUND
  Helicobacter pylori ClariRes assay is a novel commercially available real-time PCR assay allowing H. pylori detection and clarithromycin susceptibility testing in either gastric biopsy or stool specimens.
  
  
  OBJECTIVE
  The aim of this study was to validate the novel biprobe real-time assay in stool specimens from 217 dyspeptic children.
  
  
  METHODS
  DNA from gastric biopsies and stool specimens were obtained and submitted to the biprobe real time assay for H. pylori detection and clarithromycin susceptibility testing.
  
  
  RESULTS
  The sensitivity, specificity, and test accuracy were 69, 100 and 93.9% for the detection of H. pylori infection and 83.3, 100 and 95.6%, for detection of clarithromycin resistance.
  
  
  CONCLUSIONS
  This assay proved to be appropriate for H. pylori clarithromycin susceptibility testing, particularly in children populations where a high prevalence of clarithromycin-resistant strains is suspected.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Brazil. scaletsky@unifesp.br
authors.forename: Isabel C A
authors.initials: IC
authors.lastname: Scaletsky
authors.forename: Katia R S
authors.initials: KR
authors.lastname: Aranda
authors.forename: Gabriela T
authors.initials: GT
authors.lastname: Garcia
authors.forename: Manoel E P
authors.initials: ME
authors.lastname: Gonçalves
authors.forename: Silvia R
authors.initials: SR
authors.lastname: Cardoso
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Iriya
authors.forename: Neusa P
authors.initials: NP
authors.lastname: Silva
mesh_terms: D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D017291:Clarithromycin / Q000494:pharmacology*; D005243:Feces / Q000382:microbiology; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D017291:Clarithromycin