title: The plant pathogen Streptomyces scabies 87-22 has a functional pyochelin biosynthetic pathway that is regulated by TetR- and AfsR-family proteins.
abstract: Siderophores are high-affinity iron-chelating compounds produced by bacteria for iron uptake that can act as important virulence determinants for both plant and animal pathogens. Genome sequencing of the plant pathogen Streptomyces scabies 87-22 revealed the presence of a putative pyochelin biosynthetic gene cluster (PBGC). Liquid chromatography (LC)-MS analyses of culture supernatants of S. scabies mutants, in which expression of the cluster is upregulated and which lack a key biosynthetic gene from the cluster, indicated that pyochelin is a product of the PBGC. LC-MS comparisons with authentic standards on a homochiral stationary phase confirmed that pyochelin and not enantio-pyochelin (ent-pyochelin) is produced by S. scabies. Transcription of the S. scabies PBGC occurs via ~19 kb and ~3 kb operons and transcription of the ~19 kb operon is regulated by TetR- and AfsR-family proteins encoded by the cluster. This is the first report, to our knowledge, of pyochelin production by a Gram-positive bacterium; interestingly regulation of pyochelin production is distinct from characterized PBGCs in Gram-negative bacteria. Though pyochelin-mediated iron acquisition by Pseudomonas aeruginosa is important for virulence, in planta bioassays failed to demonstrate that pyochelin production by S. scabies is required for development of disease symptoms on excised potato tuber tissue or radish seedlings.
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Ryan F
authors.initials: RF
authors.lastname: Seipke
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Joanna
authors.initials: J
authors.lastname: Bicz
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Paris
authors.initials: P
authors.lastname: Laskaris
authors.affiliation: Department of Biological Sciences, University of Warwick, Coventry, UK.
authors.forename: Alice M
authors.initials: AM
authors.lastname: Yaxley
authors.affiliation: Department of Chemistry, University of Warwick, Coventry, UK.
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Challis
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Loria
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D023061:Gene Order; D008954:Models, Biological; D005810:Multigene Family; D010636:Phenols / Q000378:metabolism*; D010944:Plants / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger; D031224:Raphanus / Q000378:metabolism / Q000382:microbiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism / Q000382:microbiology; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D013844:Thiazoles / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D010636:Phenols; D012333:RNA, Messenger; D012097:Repressor Proteins; D013844:Thiazoles; D014157:Transcription Factors; C025316:pyochelin