title: Expression and prognostic significance of a new tumor metastasis suppressor gene LASS2 in human bladder carcinoma.
abstract: The LASS2 gene has been identified as a new tumor metastasis suppressor gene and has been seen to correlate with the degree of invasion and recurrence in carcinomas of prostate, breast, liver, ovarian, and pancreas. However, expression and prognostic significance of LASS2 in human bladder carcinoma are largely unknown. In this study, the protein expression of LASS2 in 80 patients with different stages was detected by immunohistochemical staining. The prognostic value of LASS2 in bladder cancers can also be assessed by a long-term follow-up investigation. The mRNA expression level of the LASS2 gene was examined using real-time quantitative PCR (qPCR) in human bladder carcinoma and paired non-tumor bladder tissues, which were obtained from 30 patients who underwent total cystectomy. We found that patients with LASS2-negative bladder cancer were linked to poor clinical prognosis. The expression of LASS2 mRNA was significantly correlated with clinical stage (P < 0.001), depth of tumor invasion (P < 0.001), and recurrence (P < 0.001). Thus, LASS2 expression may be correlated with the development and progression of human bladder carcinoma and may be a prognostic indicator for this carcinoma.
authors.affiliation: Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Jiansong
authors.initials: J
authors.lastname: Wang
authors.forename: Yigang
authors.initials: Y
authors.lastname: Zuo
authors.forename: Mingxia
authors.initials: M
authors.lastname: Ding
authors.forename: Ruping
authors.initials: R
authors.lastname: Yan
authors.forename: Delin
authors.initials: D
authors.lastname: Yang
authors.forename: Changxin
authors.initials: C
authors.lastname: Ke
mesh_terms: D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D051049:Sphingosine N-Acyltransferase / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; D025521:Tumor Suppressor Proteins; C438311:CERS2 protein, human; D051049:Sphingosine N-Acyltransferase