title: Transcriptome analysis of the duodenum in Wistar rats fed a trypsin inhibitor derived from squid viscera.
abstract: To investigate the effects of oral administration of a trypsin inhibitor (TI), normal Wistar rats were fed a TI derived from squid (Todarodes pacificus) for 10 weeks and gene expression profiles in the duodenum, pancreas, liver, and muscle were then analyzed using DNA microarrays. Although no significant changes could be observed in growth, food intake, tissue weight, or blood tests among the tissues tested, the duodenum showed the most remarkable changes in the global gene expression profile. Significant up-regulation of mRNAs encoding gastrin, gastrokine, cholecystokinin and somatostatin in the duodenum was validated by qPCR analysis. In gene ontology (GO) analysis of the up-regulated differentially expressed genes (DEGs), GO terms related to keratinization and innate mucosal defense were enriched (p < 0.001) in the category of biological processes in addition to assumable terms such as regulation of secretion and response to nutrients, vesicle-mediated transport, and so forth. In the same analysis, calcium ion binding was listed at the deepest hierarchy in the category of molecular function. These results indicate that the duodenum responds to TI treatment by a wider range of physiological processes than previously assumed such as keratinocyte differentiation and innate mucosal defense, in which calcium plays a crucial role.
authors.affiliation: Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan. kohsukeadachi@kochi-u.ac.jp
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Adachi
authors.forename: Kana
authors.initials: K
authors.lastname: Fukumorita
authors.forename: Michihiro
authors.initials: M
authors.lastname: Araki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Zaima
authors.forename: Satoru
authors.initials: S
authors.lastname: Chiba
authors.forename: Hideki
authors.initials: H
authors.lastname: Kishimura
authors.forename: Hiroki
authors.initials: H
authors.lastname: Saeki
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000235:genetics; D049832:Decapodiformes / Q000737:chemistry*; D004032:Diet; D004386:Duodenum / Q000737:chemistry*; D005755:Gastrins / Q000235:genetics; D020869:Gene Expression Profiling; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000235:genetics; D014361:Trypsin Inhibitors / Q000008:administration & dosage*; D015854:Up-Regulation
chemical_list: D005755:Gastrins; D014361:Trypsin Inhibitors; D013004:Somatostatin; D002766:Cholecystokinin