title: Amycolatopsis granulosa sp. nov., Amycolatopsis ruanii sp. nov. and Amycolatopsis thermalba sp. nov., thermophilic actinomycetes isolated from arid soils.
abstract: The taxonomic positions of three thermophilic actinomycetes isolated from arid soil samples were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus Amycolatopsis. 16S rRNA gene sequence data supported the classification of the isolates in the genus Amycolatopsis and showed that they formed distinct branches in the Amycolatopsis methanolica subclade. DNA-DNA relatedness studies between the isolates and their phylogenetic neighbours showed that they belonged to distinct genomic species. The three isolates were readily distinguished from one another and from the type strains of species classified in the A. methanolica subclade based on a combination of phenotypic properties and by genomic fingerprinting. Consequently, it is proposed that the three isolates be classified in the genus Amycolatopsis as representatives of Amycolatopsis granulosa sp. nov. (type strain GY307(T) = NCIMB 14709(T) = NRRL B-24844(T)), Amycolatopsis ruanii sp. nov. (type strain NMG112(T) = NCIMB 14711(T) = NRRL B-24848(T)) and Amycolatopsis thermalba sp. nov. (type strain SF45(T) = NCIMB 14705(T) = NRRL B-24845(T)).
authors.affiliation: Departamento de Entomologia and Acarologia, ESALQ, Universidade de São Paulo, Piracicaba, Brazil.|School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Tiago Domingues
authors.initials: TD
authors.lastname: Zucchi
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Geok Yuan Annie
authors.initials: GYA
authors.lastname: Tan
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Avinash Naga Venkata
authors.initials: ANV
authors.lastname: Bonda
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Frank
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Jenileima Devi
authors.initials: JD
authors.lastname: Kshetrimayum
authors.affiliation: School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Goodfellow
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S