title: Serum paraoxonase/arylesterase activity affects outcome in ischemic stroke patients.
abstract: BACKGROUND
  The severity of neurological deficits arising from ischemic stroke may be related to serum redox homeostasis. The aim of this study was to estimate the effect of serum paraoxonase (PON), arylesterase (ARE) activities and conjugated dienes (CD) on patient outcome during a 1-year follow-up period.
  
  
  METHODS
  The study included 468 consecutive ischemic stroke patients (251 males, 217 females) with an average age of 67.5 ± 12.4 years. Clinical evaluation was based on vital signs, National Institutes of Health Stroke Scale (NIHSS) scored at the time of admission and on the 7th day after stroke, as well as modified Rankin scale (mRS) and Barthel index (BI) scored at 30, 90, 180 and 360 days after stroke onset. Serum PON, ARE activities and CD concentration were measured with the use of spectrophotometric methods.
  
  
  RESULTS
  Serum PON activity alone correlated directly with a favorable outcome during a 3-month observation period. Serum ARE activity correlated directly only with the mRS score in a 1-year observation. PON/ARE ratio showed the strongest direct correlation with favorable stroke outcome expressed by BI and inverse correlation with mRS as compared to serum PON or ARE activities assessed alone. PON/ARE affected the NIHSS score on admission (rS = -0.119, p = 0.014) and on the 7th day after stroke (rS = 0.120, p = 0.015); it also showed an association with the BI and mRS on the 30th (rS = 0.145, p = 0.007 and rS = -0.098, p = 0.049, respectively), 90th (rS = 0.147, p = 0.009, rS = -0.133, p = 0.008, respectively), as well as 180th, and 360th day after stroke. We did not find correlations between the serum CD concentration and stroke outcome.
  
  
  CONCLUSIONS
  The PON/ARE ratio is an important predictor of ischemic stroke outcome and can be used in clinical practice rather than evaluating either PON or ARE activity alone.
authors.affiliation: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, Przybyszewskiego Str. 49, Poznan, Poland. swami622@gmail.com
authors.forename: Slawomir
authors.initials: S
authors.lastname: Michalak
authors.forename: Radoslaw
authors.initials: R
authors.lastname: Kazmierski
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Hellmann
authors.forename: Ewa
authors.initials: E
authors.lastname: Wysocka
authors.forename: Dagmara
authors.initials: D
authors.lastname: Kocialkowska-Adamczewska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wencel-Warot
authors.forename: Wiesław L
authors.initials: WL
authors.lastname: Nowinski
mesh_terms: D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011379:Prognosis; D012720:Severity of Illness Index; D020521:Stroke / Q000097:blood* / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase