title: Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology.
abstract: Present study investigated the effect of dietary supplementation of Bacillus subtilis LS 1-2 on growth performance, apparent nutrient retention, caecal microbial population and intestinal morphology in broilers. Three hundred and twenty day-old Ross broiler chicks were randomly allotted to four treatments on the basis of BW in a randomized complete block design. Experimental diets were fed in two phases: starter (d 0-21) and finisher (d 22-35). Dietary treatments were basal diet supplemented with 0% (control), 0.15%, 0.30% and 0.45% B. subtilis LS 1-2. Supplementation of increasing levels of B. subtilis LS 1-2 showed linear improvement (P<0.05) in growth performance and apparent nutrient retention. At d 35, birds supplemented with increasing levels of B. subtilis LS 1-2 showed decrease in caecal Clostridium and Coliform count (linear, P<0.05). Moreover, supplementation of B. subtilis LS 1-2 increased (linear, P<0.05) villus height and villus height to crypt depth ratio in both duodenum and ileum. Results obtained in the present study indicate that B. subtilis LS 1-2 can be used as a growth promoter in broiler diets and can improve intestinal microbial balance and gut health of broilers.
authors.affiliation: College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Sinol
authors.initials: S
authors.lastname: Sen
authors.forename: S L
authors.initials: SL
authors.lastname: Ingale
authors.forename: Y W
authors.initials: YW
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: J D
authors.initials: JD
authors.lastname: Lohakare
authors.forename: E K
authors.initials: EK
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
authors.forename: M H
authors.initials: MH
authors.lastname: Ryu
authors.forename: I K
authors.initials: IK
authors.lastname: Kwon
authors.forename: B J
authors.initials: BJ
authors.lastname: Chae
mesh_terms: D000818:Animals; D001412:Bacillus subtilis; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D004063:Digestion / Q000502:physiology; D007421:Intestine, Small / Q000033:anatomy & histology* / Q000382:microbiology; D019936:Probiotics