title: [Effects on collagen I and III after transplantation of porcine acellular dermal matrix with hyaluronic acid].
abstract: OBJECTIVE
  To investigate the changes of collagens I and III after the addition of hyaluronic acid in the transplantation of porcine acellular dermal matrix.
  
  
  METHODS
  Full-thickness skin defects were created on the dorsa of Japanese white rabbits. And the rabbits were divided randomly into 3 groups: Group A (hyaluronic acid, porcine acellular dermal matrix plus thin skin autografts), Group B (porcine acellular dermal matrix plus thin skin autografts) and Group C (skin autografts). Skin biopsies were performed at Day 50 post-grafting to detect the contents of collagens I and III by histological examinations, immunohistochemistry method and Western blot.
  
  
  RESULTS
  The areas of skin graft were (13.3 ± 1.2), (9.5 ± 0.9) and (10.0 ± 1.4) cm(2) in Groups A, B and C respectively. Group A was larger than Groups B and C(all P < 0. 01). There was no statistical difference between Groups B and C (P > 0.05). The expressions of collagen I were 1894 ± 164, 515 ± 38 and 395 ± 43 in Groups A, B and C respectively. Group A was higher than Groups B and C (P < 0.01). And the expressions of collagen III were 5411 ± 435, 874 ± 70 and 2078 ± 175 in Groups A, B and C respectively. Group C was higher than Group B and yet lower than Group A (all P < 0.01). The ratios of collagen I and collagen III in Group A (0.39) and Group B (0.59) were higher than that of Group C (0.19) (all P < 0.01).
  
  
  CONCLUSIONS
  The addition of hyaluronic acid may boost the expression of collagens I and III and decrease the ratio of collagen I/collagen III. Thus it facilitates wound healing and basilar membrane remodeling and alleviates the contraction of skin transplant.
authors.affiliation: Department of Burns & Plastic Surgery, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhao
authors.forename: Jia-Ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Song
authors.forename: Tian-Jun
authors.initials: TJ
authors.lastname: Sun
authors.forename: Dong-Jie
authors.initials: DJ
authors.lastname: Li
authors.forename: Ling-Ying
authors.initials: LY
authors.lastname: Liu
authors.forename: Quan-Wen
authors.initials: QW
authors.lastname: Gao
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Liang
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis*; D024061:Collagen Type III / Q000096:biosynthesis*; D020405:Dermis / Q000637:transplantation*; D005109:Extracellular Matrix / Q000637:transplantation*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D011817:Rabbits; D012867:Skin / Q000378:metabolism; D016038:Skin Transplantation; D013552:Swine; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D006820:Hyaluronic Acid