title: Paraoxonase 1 gene polymorphisms, paraoxonase/arylesterase activities and oxidized low-density lipoprotein levels in patients with migraine.
abstract: The vascular endothelial dysfunction has been implicated in the pathogenesis of migraine. Oxidized low-density lipoprotein (ox-LDL) may impair endothelial function. Paraoxonase-1 (PON-1) prevents oxidative modification of LDL cholesterol (LDL-C). So we investigated serum PON-1 and arylesterase (ARE) activities, PON-1 55 L/M and 192Q/R polymorphisms and the serum lipid profile in patients with migraine. Biochemical parameters and PON-1 polymorphism analyses were assessed in 104 patients with migraine and 86 healthy subjects. Ox-LDL was detected by ELISA, and polymorphisms were determined using PCR-restriction fragment length polymorphism analysis. Patients with migraine had lower PON-1 and ARE activities (p < 0·001, for both) and higher ox-LDL and LDL-C levels (p < 0·001, for both) and ox-LDL: LDL-C ratio (p < 0·005) than the controls. The genotype distribution and the allele frequencies for PON-1 55 L/M and 192Q/R polymorphisms were not different among the study populations. The results of our current study indicate that migrainous patients have decreased serum PON-1 and ARE activities and increased serum ox-LDL levels, which may have a clinical importance in the treatment of migraine.
authors.affiliation: Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, Turkey. serapyildirim10@hotmail.com
authors.forename: Serap
authors.initials: S
authors.lastname: Yıldırım
authors.forename: Sedat
authors.initials: S
authors.lastname: Akar
authors.forename: Mutlu
authors.initials: M
authors.lastname: Kuyucu
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Yıldırım
authors.forename: Senol
authors.initials: S
authors.lastname: Dane
authors.forename: Recep
authors.initials: R
authors.lastname: Aygül
mesh_terms: D000328:Adult; D000483:Alleles; D043303:Aryldialkylphosphatase / Q000097:blood* / Q000235:genetics*; D015992:Body Mass Index; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D015894:Genome, Human; D005838:Genotype; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008881:Migraine Disorders / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D010084:Oxidation-Reduction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D014421:Turkey; D055815:Young Adult
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human