title: Impact of paracrine signals from brain microvascular endothelial cells on microglial proliferation and migration.
abstract: Neuronal survival can be influenced by activated microglia, but limited evidence exists on the effects of paracrine signaling from brain microvascular endothelial cells (BMECs) on microglial action. Therefore, we examined the effects of normal BMECs conditioned medium (BMECs-CM) on activated microglia induced by pro-inflammatory cytokine macrophage inflammatory protein-1beta (MIP-1β), a chemokine that released from ischemic BMECs and has been proved to stimulate microglial proliferation. Our results showed that BMECs-CM inhibited the proliferation and transmigration of microglia induced by MIP-1β. Moreover, BMECs-CM significantly suppressed the expression of the MIP-1β receptor, CCR5, and the phosphorylation of p38 and JNK (P<0.05). These findings suggest that BMECs-CM could inhibit MIP-1β-induced microglial activation. Future therapeutic strategies that prioritize the early recovery of BMECs could be beneficial for microglial inhibition and further increase neuronal survival.
authors.affiliation: School of Preclinical Medicine, Beijing University of Chinese Medicine, Chao Yang District, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Peng-Tao
authors.initials: PT
authors.lastname: Li
authors.forename: Huan
authors.initials: H
authors.lastname: Du
authors.forename: Jin-Cai
authors.initials: JC
authors.lastname: Hou
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Li
authors.forename: Yan-Shu
authors.initials: YS
authors.lastname: Pan
authors.forename: Qian
authors.initials: Q
authors.lastname: Hua
authors.forename: Hui-Cong
authors.initials: HC
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000166:cytology* / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation / Q000502:physiology; D054407:Chemokine CCL4 / Q000494:pharmacology*; D017077:Culture Media, Conditioned / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008833:Microcirculation; D017628:Microglia / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D019899:Paracrine Communication / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019713:Receptors, CCR5 / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D054407:Chemokine CCL4; D017077:Culture Media, Conditioned; D019713:Receptors, CCR5; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases