title: Autologous transplantation of adipose-derived mesenchymal stem cells markedly reduced acute ischemia-reperfusion lung injury in a rodent model.
abstract: BACKGROUND
  This study tested the hypothesis that autologous transplantation of adipose-derived mesenchymal stem cells (ADMSCs) can effectively attenuate acute pulmonary ischemia-reperfusion (IR) injury.
  
  
  METHODS
  Adult male Sprague-Dawley (SD) rats (n = 24) were equally randomized into group 1 (sham control), group 2 (IR plus culture medium only), and group 3 (IR plus intravenous transplantation of 1.5 × 106 autologous ADMSCs at 1h, 6h, and 24h following IR injury). The duration of ischemia was 30 minutes, followed by 72 hours of reperfusion prior to sacrificing the animals. Blood samples were collected and lungs were harvested for analysis.
  
  
  RESULTS
  Blood gas analysis showed that oxygen saturation (%) was remarkably lower, whereas right ventricular systolic pressure was notably higher in group 2 than in group 3 (all p < 0.03). Histological scoring of lung parenchymal damage was notably higher in group 2 than in group 3 (all p < 0.001). Real time-PCR demonstrated remarkably higher expressions of oxidative stress, as well as inflammatory and apoptotic biomarkers in group 2 compared with group 3 (all p < 0.005). Western blot showed that vascular cell adhesion molecule (VCAM)-1, intercellular adhesion molecule (ICAM)-1, oxidative stress, tumor necrosis factor-α and nuclear factor-κB were remarkably higher, whereas NAD(P)H quinone oxidoreductase 1 and heme oxygenase-1 activities were lower in group 2 compared to those in group 3 (all p < 0.004). Immunofluorescent staining demonstrated notably higher number of CD68+ cells, but significantly fewer CD31+ and vWF+ cells in group 2 than in group 3.
  
  
  CONCLUSIONS
  ADMSC therapy minimized lung damage after IR injury in a rodent model through suppressing oxidative stress and inflammatory reaction.
authors.affiliation: Department of Emergency Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
authors.forename: Cheuk-Kwan
authors.initials: CK
authors.lastname: Sun
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Yen
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Lin
authors.forename: Tzu-Hsien
authors.initials: TH
authors.lastname: Tsai
authors.forename: Li-Teh
authors.initials: LT
authors.lastname: Chang
authors.forename: Ying-Hsien
authors.initials: YH
authors.lastname: Kao
authors.forename: Sarah
authors.initials: S
authors.lastname: Chua
authors.forename: Morgan
authors.initials: M
authors.lastname: Fu
authors.forename: Sheung-Fat
authors.initials: SF
authors.lastname: Ko
authors.forename: Steve
authors.initials: S
authors.lastname: Leu
authors.forename: Hon-Kan
authors.initials: HK
authors.lastname: Yip
mesh_terms: D055371:Acute Lung Injury / Q000150:complications* / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001158:Arteries / Q000378:metabolism / Q000503:physiopathology; D015415:Biomarkers / Q000378:metabolism; D001794:Blood Pressure; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000473:pathology / Q000503:physiopathology; D007249:Inflammation / Q000150:complications / Q000235:genetics; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D018384:Oxidative Stress / Q000235:genetics; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000150:complications* / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D014182:Transplantation, Autologous; D014661:Vasoconstriction / Q000235:genetics
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D010100:Oxygen