title: Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers.
abstract: OBJECTIVE
  To evaluate the phytochemical and anti-bacterial efficacy of the seed kernel extract of Mangifera indica (M. indica) against the enteropathogen, Shigella dysenteriae (S. dysenteriae), isolated from the diarrhoeal stool specimens.
  
  
  METHODS
  The preliminary phytochemical screening was performed by the standard methods as described by Harborne. Cold extraction method was employed to extract the bioactive compounds from mango seed kernel. Disc diffusion method was adopted to screen antibacterial activity. Minimum inhibitory concentration (MIC) was evaluated by agar dilution method. The crude extracts were partially purified by thin layer chromatography (TLC) and the fractions were analyzed by high performance thin layer chromatography (HPTLC) to identify the bioactive compounds.
  
  
  RESULTS
  Phytochemical scrutiny of M. indica indicated the presence of phytochemical constituents such as alkaloids, gums, flavanoids, phenols, saponins, steroids, tannins and xanthoproteins. Antibacterial activity was observed in two crude extracts and various fractions viz. hexane, benzene, chloroform, methanol and water. MIC of methanol fraction was found to be (95±11.8) μg/mL. MIC of other fractions ranged from 130-380 μg/mL.
  
  
  CONCLUSIONS
  The present study confirmed that each crude extracts and fractions of M. indica have significant antimicrobial activity against the isolated pathogen S. dysenteriae. The antibacterial activity may be due to the phytochemical constituents of the mango seed kernel. The phytochemical tannin could be the reason for its antibacterial activity.
authors.affiliation: Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirapalli-620 005, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Rajan
authors.forename: T
authors.initials: T
authors.lastname: Thirunalasundari
authors.forename: S
authors.initials: S
authors.lastname: Jeeva
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004405:Dysentery, Bacillary / Q000382:microbiology; D006801:Humans; D031022:Mangifera / Q000737:chemistry*; D008826:Microbial Sensitivity Tests / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D012762:Shigella dysenteriae / Q000187:drug effects* / Q000302:isolation & purification; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D013634:Tannins