title: Quantitation of drugs via molecularly imprinted polymer solid phase extraction and electrospray ionization mass spectrometry: benzodiazepines in human plasma.
abstract: The association of solid phase extraction with molecularly imprinted polymers (MIP) and electrospray ionization mass spectrometry (ESI-MS) is applied to the direct extraction and quantitation of benzodiazepines in human plasma. The target analytes are sequestered by MIP and directly analyzed by ESI-MS. Due to the MIP highly selective extraction, ionic suppression during ESI is minimized; hence no separation is necessary prior to ESI-MS, which greatly increases analytical speed. Benzodiazepines (medazepam, nitrazepam, diazepam, chlordiazepoxide, clonazepam and midazolam) in human plasma were chosen as a proof-of-principle case of drug analyses by MIP-ESI-MS in a complex matrix. MIP-ESI-MS displayed good figures of merits for medazepam, nitrazepam, diazepam, chlordiazepoxide and midazolam, with analytical calibration curves ranging from 10 to 250 μg L(-1) (r > 0.98) with limit of quantification <10 μg L(-1) and acceptable within-day and between-day precision and accuracy.
authors.affiliation: Laboratory of Toxicants and Drugs Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas-Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000, Alfenas, MG, Brazil. eduardocfig@yahoo.com.br
authors.forename: Eduardo Costa
authors.initials: EC
authors.lastname: Figueiredo
authors.forename: Regina
authors.initials: R
authors.lastname: Sparrapan
authors.forename: Gustavo Braga
authors.initials: GB
authors.lastname: Sanvido
authors.forename: Mariane Gonçalves
authors.initials: MG
authors.lastname: Santos
authors.forename: Marco Aurélio Zezzi
authors.initials: MA
authors.lastname: Arruda
authors.forename: Marcos Nogueira
authors.initials: MN
authors.lastname: Eberlin
mesh_terms: D001569:Benzodiazepines / Q000097:blood* / Q000302:isolation & purification; D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D011108:Polymers; D001569:Benzodiazepines