title: Nitrosative stress causes amino acid auxotrophy in hmp mutant Salmonella Typhimurium.
abstract: Cytotoxic nitic oxide (NO) damages various bacterial macromolecules, resulting in abnormal metabolism by mechanisms largely unknown. We show that NO can cause amino acid auxotrophy in Salmonella Typhimurium lacking major NO-metabolizing enzyme, flavohemoglobin Hmp. In NO-producing cultures, supplementation with amino acid pool restores growth of Hmp-deficient Salmonella to normal growth phases, whereas excluding Cys or BCAA Leu, Ile, or Val from amino acid pool reduces growth recovery. Data suggest that, without detoxification, NO might inactivate key enzymes in the biosynthesis pathway of amino acids essential for Salmonella replication in amino acid-limiting host environments.
authors.affiliation: Department of Microbiology and Immunology, Chosun University School of Dentistry, Gwangju 501-759, Korea.
authors.forename: Yoon Mee
authors.initials: YM
authors.lastname: Park
authors.forename: Hee Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Young Hee
authors.initials: YH
authors.lastname: Joung
authors.forename: Iel Soo
authors.initials: IS
authors.lastname: Bang
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006420:Hemeproteins / Q000172:deficiency* / Q000235:genetics; D009154:Mutation; D009569:Nitric Oxide / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D006420:Hemeproteins; C086193:flavohemoprotein, Bacteria; D009569:Nitric Oxide