title: A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax.
abstract: Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.
authors.affiliation: Singapore Immunology Network, Biopolis, Agency for Science Technology and Research, Singapore. bruce_russell@immunol.a-star.edu.sg
authors.forename: Bruce
authors.initials: B
authors.lastname: Russell
authors.forename: Rossarin
authors.initials: R
authors.lastname: Suwanarusk
authors.forename: Céline
authors.initials: C
authors.lastname: Borlon
authors.forename: Fabio T M
authors.initials: FT
authors.lastname: Costa
authors.forename: Cindy S
authors.initials: CS
authors.lastname: Chu
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Rijken
authors.forename: Kanlaya
authors.initials: K
authors.lastname: Sriprawat
authors.forename: Lucile
authors.initials: L
authors.lastname: Warter
authors.forename: Esther G L
authors.initials: EG
authors.lastname: Koh
authors.forename: Benoit
authors.initials: B
authors.lastname: Malleret
authors.forename: Yves
authors.initials: Y
authors.lastname: Colin
authors.forename: Olivier
authors.initials: O
authors.lastname: Bertrand
authors.forename: John H
authors.initials: JH
authors.lastname: Adams
authors.forename: Umberto
authors.initials: U
authors.lastname: D'Alessandro
authors.forename: Georges
authors.initials: G
authors.lastname: Snounou
authors.forename: Francois
authors.initials: F
authors.lastname: Nosten
authors.forename: Laurent
authors.initials: L
authors.lastname: Rénia
mesh_terms: D002478:Cells, Cultured; D006403:Hematologic Tests / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods* / Q000592:standards; D054884:Host-Pathogen Interactions; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology; D010966:Plasmodium vivax / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology*; D015203:Reproducibility of Results; D012156:Reticulocytes / Q000469:parasitology*