title: On-chip DNA methylation analysis using osmium complexation.
abstract: The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.
authors.affiliation: Nucleic Acid Chemistry Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Umemoto
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto