title: Recombinant mussel adhesive protein fp-5 (MAP fp-5) as a bulk bioadhesive and surface coating material.
abstract: Mussel adhesive proteins (MAPs) attach to all types of inorganic and organic surfaces, even in wet environments. MAP of type 5 (fp-5), in particular, has been considered as a key adhesive material. However, the low availability of fp-5 has hampered its biochemical characterization and practical applications. Here, soluble recombinant fp-5 is mass-produced in Escherichia coli. Tyrosinase-modified recombinant fp-5 showed ∼1.11 MPa adhesive shear strength, which is the first report of a bulk-scale adhesive force measurement for purified recombinant of natural MAP type. Surface coatings were also performed through simple dip-coating of various objects. In addition, complex coacervate using recombinant fp-5 and hyaluronic acid was prepared as an efficient adhesive formulation, which greatly improved the bulk adhesive strength. Collectively, it is expected that this work will enhance basic understanding of mussel adhesion and that recombinant fp-5 can be successfully used as a realistic bulk-scale bioadhesive and an efficient surface coating material.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors.forename: Yoo Seong
authors.initials: YS
authors.lastname: Choi
authors.forename: Dong Gyun
authors.initials: DG
authors.lastname: Kang
authors.forename: Seonghye
authors.initials: S
authors.lastname: Lim
authors.forename: Yun Jung
authors.initials: YJ
authors.lastname: Yang
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Kim
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000818:Animals; D049872:Bivalvia; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004926:Escherichia coli; D006820:Hyaluronic Acid / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014014:Tissue Adhesives / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011506:Proteins; D011994:Recombinant Proteins; D014014:Tissue Adhesives; C037403:adhesive protein, mussel; D006820:Hyaluronic Acid; D014442:Monophenol Monooxygenase