title: Combination of polymorphisms in luteinizing hormone β, estrogen receptor β and progesterone receptor and susceptibility to infertility and endometriosis.
abstract: OBJECTIVE
  To determine whether the combination of PR (PROGINS), ERβ G+1730A and/or LHβ G1502A polymorphisms in infertile women with and without endometriosis and in a control group increases the risk of infertility and/or endometriosis.
  
  
  METHODS
  Case-control study including 201 infertile women with endometriosis, 80 infertile women without endometriosis and 206 fertile women as control group. PROGINS was identified by PCR (polymerase chain reaction) and ERβ G+1730A and LHβ G1502A were identified by PCR-RFLP (restriction fragment length polymorphism).
  
  
  RESULTS
  A statistically significant difference was found for the combination of LHβ+ERβ polymorphisms among infertile patients with endometriosis and control group (p=0.003, OR=2.468), among infertile patients with endometriosis I/II and control group (p=0.002, OR=3.081), among infertile patients with endometriosis III/IV and control group (p=0.035, OR=2.136) and for the combination of LHβ+PROGINS polymorphisms among infertile patients with endometriosis I/II and control group (p=0.014, OR=3.081). However, the odds of developing endometriosis are not enhanced in the presence of the two polymorphisms, being similar to the odds when only LH polymorphism is present.
  
  
  CONCLUSIONS
  Individually, the presence of LHβ G1502A and ERβ G+1730A polymorphisms is associated with infertility and endometriosis associated infertility. However, when two polymorphisms are present in the same individual it does not appear to increase the chance of developing endometriosis or infertility.
authors.affiliation: Department of Gynecology and Obstetrics, Faculdade de Medicina do ABC, Santo André, SP, Brazil.
authors.forename: Denise Maria
authors.initials: DM
authors.lastname: Christofolini
authors.forename: Fábia Lima
authors.initials: FL
authors.lastname: Vilarino
authors.forename: Fernanda Abani
authors.initials: FA
authors.lastname: Mafra
authors.forename: Gustavo Mendonça
authors.initials: GM
authors.lastname: André
authors.forename: Bianca
authors.initials: B
authors.lastname: Bianco
authors.forename: Caio Parente
authors.initials: CP
authors.lastname: Barbosa
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D011110:Polymorphism, Genetic; D011980:Receptors, Progesterone / Q000235:genetics*
chemical_list: D047629:Estrogen Receptor beta; D037101:Luteinizing Hormone, beta Subunit; D011980:Receptors, Progesterone