title: Transcriptional and post-transcriptional regulation of a NAC1 transcription factor in Medicago truncatula roots.
abstract: • Legume roots develop two types of lateral organs, lateral roots and nodules. Nodules develop as a result of a symbiotic interaction with rhizobia and provide a niche for the bacteria to fix atmospheric nitrogen for the plant. • The Arabidopsis NAC1 transcription factor is involved in lateral root formation, and is regulated post-transcriptionally by miRNA164 and by SINAT5-dependent ubiquitination. We analyzed in Medicago truncatula the role of the closest NAC1 homolog in lateral root formation and in nodulation. • MtNAC1 shows a different expression pattern in response to auxin than its Arabidopsis homolog and no changes in lateral root number or nodulation were observed in plants affected in MtNAC1 expression. In addition, no interaction was found with SINA E3 ligases, suggesting that post-translational regulation of MtNAC1 does not occur in M. truncatula. Similar to what was found in Arabidopsis, a conserved miR164 target site was retrieved in MtNAC1, which reduced protein accumulation of a GFP-miR164 sensor. Furthermore, miR164 and MtNAC1 show an overlapping expression pattern in symbiotic nodules, and overexpression of this miRNA led to a reduction in nodule number. • This work suggests that regulatory pathways controlling a conserved transcription factor are complex and divergent between M. truncatula and Arabidopsis.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: D'haeseleer
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Griet
authors.initials: G
authors.lastname: Den Herder
authors.affiliation: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette Cedex, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Laffont
authors.affiliation: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette Cedex, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Plet
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Mortier
authors.affiliation: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette Cedex, France.|Université Paris Diderot Paris 7, 75205 Paris Cedex 13, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Lelandais-Brière
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Stefanie
authors.initials: S
authors.lastname: De Bodt
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Annick
authors.initials: A
authors.lastname: De Keyser
authors.affiliation: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette Cedex, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Crespi
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Marcelle
authors.initials: M
authors.lastname: Holsters
authors.affiliation: Institut des Sciences du Végétal (ISV), Centre National de la Recherche Scientifique (CNRS), 91198 Gif sur Yvette Cedex, France.
authors.forename: Florian
authors.initials: F
authors.lastname: Frugier
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.|Department of Plant Biotechnology and Genetics, Ghent University, 9052 Gent, Belgium.
authors.forename: Sofie
authors.initials: S
authors.lastname: Goormachtig
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology; D046913:Medicago truncatula / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055170:Plant Root Nodulation / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017434:Protein Structure, Tertiary; D018749:RNA, Plant / Q000235:genetics; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D035683:MicroRNAs; C418004:NAC1 protein, Arabidopsis; D010940:Plant Proteins; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014157:Transcription Factors