title: Immunogenic peptides from phage display libraries with potential of protecting mice against the Pseudorabies virus.
abstract: Phage display selection of combinatorial peptide libraries has demonstrated its almost unlimited potential in identifying binding ligands for many targets. The method shows promise for selection of immunogenic peptides against pathogens by antibodies. We have undertaken a study designed to select such mimics for one of the representatives of Herpesviridae, the Pseudorabies virus (PrV), infecting pigs and causing severe neurological complications known as Aujeszky's disease. By screening a 12mer linear and a 7mer cysteine-constrained libraries with immunoglobulins of a rabbit immunized with the virus, a family of 10 antigenic and immunogenic peptides was derived sharing a sequence motif K(L/P/V)GDP(R/K/L). Groups of six C57BL/6 mice were immunized with bacteriophages expressing peptides with this motif sequences. Some of the mice were found to be positive in seroneutralization assay; in a challenge setting, all but two immunized mice survived, albeit presenting some disease symptoms. We discuss the perspectives and limits of generating peptide leads by library screening with immune polyclonal antiserum for designing pure epitope-based vaccines to PrV in the future.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, México DF, 04510, Mexico. karlen@servidor.unam.mx
authors.forename: Karlen
authors.initials: K
authors.lastname: Gazarian
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gazarian
authors.forename: José Iván Sánchez
authors.initials: JI
authors.lastname: Betancourt
authors.forename: Rogelio A
authors.initials: RA
authors.lastname: Alonso Morales
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001435:Bacteriophages / Q000276:immunology*; D016384:Consensus Sequence; D011558:Herpesvirus 1, Suid / Q000472:pathogenicity*; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D019151:Peptide Library; D010455:Peptides / Q000276:immunology*; D011557:Pseudorabies / Q000276:immunology / Q000517:prevention & control*; D011817:Rabbits; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control*; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D019151:Peptide Library; D010455:Peptides; D014759:Viral Envelope Proteins; C089580:glycoprotein E, Suid herpesvirus 1