title: Photocatalytical reduction of disulphide bonds in peptides on Ag-loaded nano-TiO2 for subsequent derivatization and determination.
abstract: We reported an alternative strategy to reduce disulphide bonds in peptides with Ag-nanoparticle loaded nano-TiO(2) (Ag/TiO(2)) under UV irradiation. The feasibility of this strategy was adequately demonstrated using the model peptides oxidized glutathione, vasopressin and insulin, which contain various disulphide bonds, as well as by its application to the determination of Cd-induced phytochelatins in Phaeodactylum tricornutum.
authors.affiliation: Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yan
authors.forename: Chunlai
authors.initials: C
authors.lastname: Xu
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Xiao
authors.forename: Limin
authors.initials: L
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Qiuquan
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002104:Cadmium / Q000378:metabolism; D002384:Catalysis; D017377:Diatoms / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D055668:Photochemical Processes; D054811:Phytochelatins / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays; D014667:Vasopressins / Q000737:chemistry
chemical_list: D004220:Disulfides; D007328:Insulin; D010455:Peptides; D002104:Cadmium; D014667:Vasopressins; C009495:titanium dioxide; D012834:Silver; D054811:Phytochelatins; D004228:Dithionitrobenzoic Acid; D014025:Titanium; D005978:Glutathione