title: Genotyping of Toxoplasma gondii isolates from cats in different geographic regions of China.
abstract: Fourteen isolates of Toxoplasma gondii were isolated from cats from 4 different geographic provinces (Anhui, Hubei, Shanxi and Guangdong) in China and their genetic diversity with 8 nuclear loci SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico, was analysed by restriction fragment length polymorphisms (RFLPs). Two genotypes from these 14 isolates were identified but none of them belongs to the typical genetic types (types I, II and III). It is unexpected that such high similarity was observed in these 14 isolates although their original regions are significantly distant. Our results strongly indicate that the three traditional clonal lineages of types I, II and III of this parasite may not be preponderant in China. In addition, our results show that the genotypes of T. gondii in China may be highly clonal with atypical genotypes and higher virulence.
authors.affiliation: Department of Parasitology and Key Laboratories of Pathogen Biology and Zoonoses, Anhui Medical University, Hefei 230032, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Chen
authors.forename: J M
authors.initials: JM
authors.lastname: Gao
authors.forename: X X
authors.initials: XX
authors.lastname: Huo
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.forename: F
authors.initials: F
authors.lastname: Halm-Lai
authors.forename: Y H
authors.initials: YH
authors.lastname: Xu
authors.forename: W J
authors.initials: WJ
authors.lastname: Song
authors.forename: G
authors.initials: G
authors.lastname: Hide
authors.forename: J L
authors.initials: JL
authors.lastname: Shen
authors.forename: Z R
authors.initials: ZR
authors.lastname: Lun
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000662:veterinary; D002371:Cat Diseases / Q000401:mortality / Q000469:parasitology*; D002415:Cats; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D060005:Genotyping Techniques / Q000662:veterinary; D005843:Geography; D051379:Mice; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D014122:Toxoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000401:mortality / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan