title: [Connection of magnetic antisense probe with SK-Br-3 oncocyte mRNA nucleotide detected by high resolution atomic force microscope].
abstract: The present paper is aimed to detect superparamagnetic iron oxide labeled c-erbB2 oncogene antisense oligonucleotide probe (magnetic antisense probe) connected with SK-Br-3 oncocyte mRNA nucleotide by high resolution atomic force microscope (AFM). We transfected SK-Br-3 oncocyte with magnetic antisense probe, then observed the cells by AFM with high resolution and detected protein expression and magnetic resonance imagine (MRI). The high resolution AFM clearly showed the connection of the oligonucleotide remote end of magnetic antisense probe with the mRNA nucleotide of oncocyte. The expression of e-erbB2 protein in SK-Br3 cells were highly inhibited by using magnetic antisense probe. We then obtained the lowest signal to noise ratio (SNR) of SK-Br-3 oncocyte transfected with magnetic antisense probe by MRI (P<0.05). These experiments demonstrated that the high resolution AFM could be used to show the binding of magnetic antisense probe and SK-Br-3 mRNA of tumor cell nuclear.
authors.affiliation: Department of Radiology, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shude
authors.initials: S
authors.lastname: Tan
authors.forename: Yu
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Xinyou
authors.initials: X
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Wen
authors.forename: Shaolin
authors.initials: S
authors.lastname: Li
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000737:chemistry* / Q000235:genetics; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D008280:Magnetics; D018625:Microscopy, Atomic Force / Q000379:methods*; D015336:Molecular Probe Techniques; D015341:Nucleic Acid Probes / Q000737:chemistry* / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D024862:Oxyphil Cells / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D016373:DNA, Antisense; D005290:Ferric Compounds; D015341:Nucleic Acid Probes; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C000499:ferric oxide