title: Negative feedback from integrins to cadherins: a micromechanical study.
abstract: The coupling between cell-cell and cell-matrix adhesion systems is known to affect the stability of the adhesive status of cells, as well as tissue cohesion. In this work, we perform quantitative assays of integrin-cadherin cross talk in controlled and reproducible conditions. This is achieved by plating cells on microprinted fibronectin patterns of different sizes, and simulating the formation of an intercellular contact with a microbead coated with E-cadherin extracellular domains and brought to the cell membrane. Using an optical trap, we measure the average rigidity modulus of the E-cadherin bead-cell contact as a function of the contact incubation time and of the cell spreading area. For a given incubation time, this rigidity modulus decreases by three orders of magnitude as the cell-matrix contact area, A, increases from 100 to 700 μm(2). In a similar way, the dynamics of formation of the bead-cell contact gets slower as this area increases. This is clear evidence for a strong negative feedback from cell-fibronectin onto cell-cell adhesive contacts, for which we discuss some possible mechanisms.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR CNRS 7057 Université Paris-Diderot, Paris, France.
authors.forename: Alia
authors.initials: A
authors.lastname: Al-Kilani
authors.forename: Olivier
authors.initials: O
authors.lastname: de Freitas
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dufour
authors.forename: François
authors.initials: F
authors.lastname: Gallet
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000187:drug effects; D015820:Cadherins / Q000378:metabolism*; D002232:Carbocyanines / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D055119:Elastic Modulus / Q000187:drug effects; D025461:Feedback, Physiological / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D016023:Integrins / Q000378:metabolism*; D051379:Mice; D008863:Microspheres; D009842:Oligopeptides / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D015820:Cadherins; D002232:Carbocyanines; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; C089187:cyanine dye 3; D049452:Green Fluorescent Proteins; D011107:Polylysine; D004533:Egtazic Acid; C047981:arginyl-glycyl-aspartic acid