title: Inhibitory effects of extracts from the marine alga Caulerpa taxifolia and of toxin from Caulerpa racemosa on multixenobiotic resistance in the marine sponge Geodia cydonium.
abstract: The invasive growth of the introduced green alga Caulerpa taxifolia, already affecting the richness and diversity of the littoral ecosystems, has become a major ecological problem in the Mediterranean Sea. Previously, we demonstrated that the water pollutant tributyltin induces apoptosis in tissue of the marine sponge Geodia cydonium at concentrations of 3 μM and higher. Here we show that exposure of G. cydonium to low (non-toxic) concentrations of Caulerpa extract or purified caulerpin (10 μg/ml) together with low doses of tributyltin (1 μM; non-toxic), results in a strong apoptotic effect. Evidence is presented that the enhancement of toxicity of tributyltin by Caulerpa extract is at least partially caused by inhibition of the multixenobiotic resistance (MXR) pump by the algal toxin. Caulerpa extract, as well as caulerpin, strongly enhance the accumulation of the test substrate of MXR, rhodamine B, in the gills of the mussel Dreissena polymorpha, used as a model system for testing MXR-inhibiting potential.
authors.affiliation: Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Duesbergweg 6, 55099 Mainz, Germany.
authors.forename: H C
authors.initials: HC
authors.lastname: Schröder
authors.forename: F A
authors.initials: FA
authors.lastname: Badria
authors.forename: S N
authors.initials: SN
authors.lastname: Ayyad
authors.forename: R
authors.initials: R
authors.lastname: Batel
authors.forename: M
authors.initials: M
authors.lastname: Wiens
authors.forename: H M
authors.initials: HM
authors.lastname: Hassanein
authors.forename: B
authors.initials: B
authors.lastname: Kurelec
authors.forename: W E
authors.initials: WE
authors.lastname: Müller