title: [The biological effect of high glucose on human periodontal ligament fibroblast].
abstract: OBJECTIVE
  The investigate the effect of high glucose on proliferation, total protein synthesis, alkaline phosphatase (ALP) activities, collagen-I(Col-I) and osteocalcin(OCN) secretion of human periodontal ligament fibroblast(hPDLF) in vitro and the regulative function of insulin in this process.
  
  
  METHODS
  Glucose of different concentration (5.5,15,25,35and 45mmol/L) was used to culture hPDLF and insulin was used for each group as treatment control. 24 hrs after administration, cell proliferation was detected by cell counting kit (CCK-8), total protein synthesis, ALP activities, Col-I and OCN secretion were investigated. Data was analyzed with SPSS 13.0 soft ware package.
  
  
  RESULTS
  Glucose of low concentration had no effect on biological function of hPDLF, but glucose of high concentration could inhibit proliferation, total protein synthesis, ALP activities, Col-I and OCN secretion of hPDLF significantly in a concentration-dependent manner. This effect could be blocked by insulin.
  
  
  CONCLUSIONS
  High glucose could inhibit biological function of hPDLF and such effect could be blocked by insulin.
authors.affiliation: Department of Stomatology, General Hospital of Chinese PLA. Beijing 100853, China. liujqmj@163.com
authors.forename: Jia-qiang
authors.initials: JQ
authors.lastname: Liu
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Feng
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts; D005947:Glucose; D006801:Humans; D015675:Osteocalcin; D010513:Periodontal Ligament
chemical_list: D015675:Osteocalcin; D005947:Glucose