title: Inhibition of dynamin by dynole 34-2 induces cell death following cytokinesis failure in cancer cells.
abstract: Inhibitors of mitotic proteins such as Aurora kinase and polo-like kinase have shown promise in preclinical or early clinical development for cancer treatment. We have reported that the MiTMAB class of dynamin small molecule inhibitors are new antimitotic agents with a novel mechanism of action, blocking cytokinesis. Here, we examined 5 of the most potent of a new series of dynamin GTPase inhibitors called dynoles. They all induced cytokinesis failure at the point of abscission, consistent with inhibition of dynamin while not affecting other cell cycle stages. All 5 dynoles inhibited cell proliferation (MTT and colony formation assays) in 11 cancer cell lines. The most potent GTPase inhibitor, dynole 34-2, also induced apoptosis, as revealed by cell blebbing, DNA fragmentation, and PARP cleavage. Cell death was induced specifically following cytokinesis failure, suggesting that dynole 34-2 selectively targets dividing cells. Dividing HeLa cells were more sensitive to the antiproliferative properties of all 5 dynoles compared with nondividing cells, and nontumorigenic fibroblasts were less sensitive to cell death induced by dynole 34-2. Thus, the dynoles are a second class of dynamin GTPase inhibitors, with dynole 34-2 as the lead compound, that are novel antimitotic compounds acting specifically at the abscission stage.
authors.affiliation: Children's Medical Research Institute, The University of Sydney, Locked Bag 23, Wentworthville, NSW 2145, Australia. mchircop@cmri.org.au
authors.forename: Megan
authors.initials: M
authors.lastname: Chircop
authors.forename: Swetha
authors.initials: S
authors.lastname: Perera
authors.forename: Anna
authors.initials: A
authors.lastname: Mariana
authors.forename: Hui
authors.initials: H
authors.lastname: Lau
authors.forename: Maggie P C
authors.initials: MP
authors.lastname: Ma
authors.forename: Jayne
authors.initials: J
authors.lastname: Gilbert
authors.forename: Nigel C
authors.initials: NC
authors.lastname: Jones
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Gordon
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Young
authors.forename: Andrew
authors.initials: A
authors.lastname: Morokoff
authors.forename: Jennette
authors.initials: J
authors.lastname: Sakoff
authors.forename: Terence J
authors.initials: TJ
authors.lastname: O'Brien
authors.forename: Adam
authors.initials: A
authors.lastname: McCluskey
authors.forename: Phillip J
authors.initials: PJ
authors.lastname: Robinson
mesh_terms: D000178:Acrylamides / Q000494:pharmacology*; D000818:Animals; D050256:Antimitotic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D065095:Calcineurin Inhibitors; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D048749:Cytokinesis / Q000187:drug effects*; D034281:Dynamins / Q000037:antagonists & inhibitors*; D005347:Fibroblasts / Q000187:drug effects; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D009369:Neoplasms / Q000201:enzymology; D011123:Polyploidy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D014404:Tubulin
chemical_list: C541935:2-cyano-N-octyl-3-(1-(3-dimethylaminopropyl)-1H-indol-3-yl)acrylamide; D000178:Acrylamides; D050256:Antimitotic Agents; D000970:Antineoplastic Agents; D065095:Calcineurin Inhibitors; D007211:Indoles; D047428:Protein Kinase Inhibitors; D011804:Quinolines; C512984:RO 3306; D013844:Thiazoles; D014404:Tubulin; D034281:Dynamins