title: Early and late effects of bone marrow-derived mononuclear cell therapy on lung and distal organs in experimental sepsis.
abstract: We tested the hypothesis that bone marrow-derived mononuclear cells (BMDMCs) at an early phase of cecal ligation and puncture (CLP)-induced sepsis may have lasting effects on: (1) lung mechanics and histology, (2) the structural remodelling of lung parenchyma, (3) lung, kidney, and liver cell apoptosis, and (4) pro- and anti-inflammatory cytokines and growth factors. At day 1, BMDMC significantly reduced mortality, as well as caspase-3, interleukin (IL)-6 and IL-1β, vascular endothelial growth factor, platelet-derived growth factor, hepatocyte growth factor, and transforming growth factor-β, but increased IL-10 mRNA expression in lung tissue in septic mice contributing to endothelium and epithelium alveolar repair and improvement of lung mechanics. BMDMC also prevented the increase of apoptotic cells in lung, liver, and kidney. At day 7, these early functional and morphological effects were preserved or further improved. In conclusion, in the present model of sepsis, the beneficial effects of early administration of BMDMCs on lung and distal organs were preserved, possibly by paracrine mechanisms.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Debora S
authors.initials: DS
authors.lastname: Ornellas
authors.forename: Tatiana
authors.initials: T
authors.lastname: Maron-Gutierrez
authors.forename: Felipe M
authors.initials: FM
authors.lastname: Ornellas
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Cruz
authors.forename: Gisele P
authors.initials: GP
authors.lastname: Oliveira
authors.forename: Isabela H
authors.initials: IH
authors.lastname: Lucas
authors.forename: Livia
authors.initials: L
authors.lastname: Fujisaki
authors.forename: Mariana G
authors.initials: MG
authors.lastname: Oliveira
authors.forename: Walcy R
authors.initials: WR
authors.lastname: Teodoro
authors.forename: Vera L
authors.initials: VL
authors.lastname: Capelozzi
authors.forename: Paolo
authors.initials: P
authors.lastname: Pelosi
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods; D017690:Cell Transplantation / Q000379:methods; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D008168:Lung / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018805:Sepsis / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D013997:Time Factors
chemical_list: D016207:Cytokines