title: Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill.
abstract: One water-soluble polysaccharide (ABP-W1) was purified from the fruiting bodies of Agaricus blazei by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Its molecular weight was about 3.9×10(2) kDa as determined by high-performance size-exclusion chromatography (HPSEC). The structural feature of ABP-W1 was investigated by a combination of chemical and instrumental analysis, including partial hydrolysis with acid, periodate oxidation-Smith degradation, acetylation, methylation analysis and nuclear magnetic resonance spectroscopy (NMR (1)H, (13)C). The results revealed that ABP-W1 had a backbone consisting of (1→6)-linked-α-D-galactopyranosyl and (1→2,6)-linked-α-D-glucopyranosyl, which was branched with one single terminal (1→)-α-D-glucopyranosyl at the O-2 position of (1→2,6)-linked-α-D-glucopyranosyl along the main chain in the ratio of 1:1:1. The observation of the complex-formation between ABP-W1 and Congo Red indicated that ABP-W1 probably existed in a triple-strand helical conformation in water. Based on the data obtained, ABP-W1 was composed of a repeating unit with a structure as below: [structure: see text].
authors.affiliation: Qiqihar Medical University, Qiqihar, PR China. jichengliu@yahoo.cn
authors.forename: Jicheng
authors.initials: J
authors.lastname: Liu
authors.forename: Chunjing
authors.initials: C
authors.lastname: Zhang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Wang
authors.forename: Haitao
authors.initials: H
authors.lastname: Yu
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Xiuzhen
authors.initials: X
authors.lastname: Yang
authors.forename: Zhecheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Xianchun
authors.initials: X
authors.lastname: Wen
authors.forename: Yongxu
authors.initials: Y
authors.lastname: Sun
authors.forename: Chunlei
authors.initials: C
authors.lastname: Yu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D002850:Chromatography, Gel; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D008745:Methylation; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011134:Polysaccharides