title: Population genetic structure of Culex quinquefasciatus in India by ISSR marker.
abstract: OBJECTIVE
  To characterize the genetic structure of various populations of Culex quinquefasciatus (Cx. quinquefasciatus) from India representing different geoclimatic locations.
  
  
  METHODS
  Inter simple sequence repeat (ISSR) markers were used. A set of 20 primers were screened with the laboratory populations of mosquito species. Finally the IS 40 primer was chosen based on the scorable banding pattern showing 100 percent polymorphism among the various populations. The statistical analysis was done using POPGENE 1.31 software. The consensus tree was generated based on UPGMA modified from NEIGHBOR procedure of PHYLIP Version 3.5.
  
  
  RESULTS
  The cluster analysis shows the main cluster which is divided into two sub cluster representing all the populations separated as per their phylogeographic and geoclimatic condition.
  
  
  CONCLUSIONS
  The findings will be helpful in understanding the population variation under different ecological conditions and development of effective vector management strategies.
authors.affiliation: Entomology Division, Defence R & D Establishment, Gwalior, Madhya Pradesh, India.
authors.forename: M J
authors.initials: MJ
authors.lastname: Mendki
authors.forename: A K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer
authors.forename: O P
authors.initials: OP
authors.lastname: Agrawal
authors.forename: Shri
authors.initials: S
authors.lastname: Prakash
authors.forename: B D
authors.initials: BD
authors.lastname: Parashar
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D003465:Culex / Q000145:classification / Q000235:genetics*; D004463:Ecology; D014644:Genetic Variation; D005828:Genetics, Population; D007194:India; D007303:Insect Vectors; D018895:Microsatellite Repeats; D010802:Phylogeny