title: Haemonchus contortus protease inhibition by n-alkyl ferulates from Maprounea guianensis.
abstract: This report describes the inhibition of the proteolytic activity of Haemonchus contortus lysate by a mixture of n-alkyl ferulates isolated from Maprounea guianensis. These compounds were evaluated in vitro for the ability to inhibit the H. contortus proteases using SDS-PAGE copolymerized with gelatine (zymography) and by assaying the hemoglobinolytic activity of these enzymes in solution. The ferulates abrogated the proteolysis compared to E-64, suggesting the activity is the result of a cysteine protease.
authors.affiliation: Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: S C O
authors.initials: SC
authors.lastname: Santos
authors.forename: J A
authors.initials: JA
authors.lastname: López
authors.forename: M
authors.initials: M
authors.lastname: Meira
authors.forename: M T B
authors.initials: MT
authors.lastname: Guedes
authors.forename: J M
authors.initials: JM
authors.lastname: David
authors.forename: F
authors.initials: F
authors.lastname: Zacharias
authors.forename: J P
authors.initials: JP
authors.lastname: David
authors.forename: F W M
authors.initials: FW
authors.lastname: Lima
mesh_terms: D000818:Animals; D003373:Coumaric Acids / Q000737:chemistry*; D019665:Euphorbiaceae / Q000737:chemistry*; D006189:Haemonchus / Q000201:enzymology*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems / Q000737:chemistry; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D003373:Coumaric Acids; D010936:Plant Extracts; D011480:Protease Inhibitors; C004999:ferulic acid