title: Effects of Panicum miliaceum L. extract on adipogenic transcription factors and fatty acid accumulation in 3T3-L1 adipocytes.
abstract: The dietary intake of whole grains is known to reduce the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. To investigate whether there are anti-adipogenic activities in various Korean cereals, we assessed water extracts of nine cereals. The results showed that treatment of 3T3-L1 adipocytes with Sorghum bicolor L. Moench, Setaria italica Beauvois, or Panicum miliaceum L. extract significantly inhibited adipocyte differentiation, as determined by measuring oil red-O staining, triglyceride accumulation, and glycerol 3-phosphate dehydrogenase activity. Among the nine cereals, P. miliaceum L. showed the highest anti-adipogenic activity. The effects of P. miliaceum L. on mRNA expression of peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1, and the CCAAT/enhancer binding protein-α were evaluated, revealing that the extract significantly decreased the expression of these genes in a dose-dependent manner. Moreover, P. miliaceum L. extract changed the ratio of monounsaturated fatty acids to saturated fatty acids in adipocytes, which is related to biological activity and cell characteristics. These results suggest that some cereals efficiently suppress adipogenesis in 3T3-L1 adipocytes. In particular, the effect of P. miliaceum L. on adipocyte differentiation is associated with the downregulation of adipogenic genes and fatty acid accumulation in adipocytes.
authors.affiliation: Functional Food & Nutrition Division, National Academy of Agricultural Science, Rural Development Administration, 150 Suin-ro, Gwonseon-gu, Suwon, Gyeonggi 441-707, Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Park
authors.forename: Dong-Won
authors.initials: DW
authors.lastname: Seo
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Sung
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Lee
authors.forename: Hwan-Hee
authors.initials: HH
authors.lastname: Jang
authors.forename: Hae-Yeon
authors.initials: HY
authors.lastname: Choi
authors.forename: Jae-Hyn
authors.initials: JH
authors.lastname: Kim
authors.forename: Dong-Sik
authors.initials: DS
authors.lastname: Park