title: Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: uncovering cross-talk of signaling components.
abstract: The present study focused on comparative proteome analyses of low- and high-temperature stresses and potential protein-protein interaction networks, constructed by using a bioinformatics approach, in response to both stress conditions.The data revealed two important points: first, the results indicate that low-temperature stress is tightly linked with oxidative stress as well as photosynthesis; however, no specific mechanism is revealed in the case of the high-temperature stress response. Second, temperature stress was revealed to be linked with nitrogen and ammonia assimilation. Moreover, the data also highlighted the cross-talk of signaling pathways. Some of the detected signaling proteins, e.g., Hik14, Hik26 and Hik28, have potential interactions with differentially expressed proteins identified in both temperature stress conditions. Some differentially expressed proteins found in the Spirulina protein-protein interaction network were also examined for their physical interactions by a yeast two hybrid system (Y2H). The Y2H results obtained in this study suggests that the potential PPI network gives quite reliable potential interactions for Spirulina. Therefore, the bioinformatics approach employed in this study helps in the analysis of phenomena where proteome analyses of knockout mutants have not been carried out to directly examine for specificity or cross-talk of signaling components.
authors.affiliation: BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Pavinee
authors.initials: P
authors.lastname: Kurdrid
authors.affiliation: BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Jittisak
authors.initials: J
authors.lastname: Senachak
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Matura
authors.initials: M
authors.lastname: Sirijuntarut
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Rayakorn
authors.initials: R
authors.lastname: Yutthanasirikul
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Phuttawadee
authors.initials: P
authors.lastname: Phuengcharoen
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Wattana
authors.initials: W
authors.lastname: Jeamton
authors.affiliation: BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Supapon
authors.initials: S
authors.lastname: Cheevadhanarak
authors.affiliation: BEC Unit, National Center for Genetic Engineering and Biotechnology, 49 Soi Theintalay 25, Thakham, Bangkhuntien, Bangkok, 10150, Thailand.
authors.forename: Apiradee
authors.initials: A
authors.lastname: Hongsthong