title: Beyond hematoxylin and eosin: the importance of immunohistochemical techniques for evaluating surgically resected constipated patients.
abstract: Chronic constipation requiring surgical ablation for intractability is often a frustrating condition from the pathologist's point of view. In fact, limiting the histological examination to only hematoxylin-eosin staining usually yields only the information that there are no abnormalities. By employing some simple and widely available immunohistochemical methods, discussed in this review, it is possible to gather data that may help in explaining the pathophysiological basis of constipation in these patients.
authors.affiliation: Gastroenterology and Hepatology Section, Department of Clinical and Experimental Medicine, University of Perugia, Perugia, Italy. gabassot@tin.it
authors.forename: G
authors.initials: G
authors.lastname: Bassotti
authors.forename: V
authors.initials: V
authors.lastname: Villanacci
authors.forename: B
authors.initials: B
authors.lastname: Salerni
authors.forename: C A
authors.initials: CA
authors.lastname: Maurer
authors.forename: G
authors.initials: G
authors.lastname: Cathomas
mesh_terms: D004396:Coloring Agents; D003248:Constipation / Q000473:pathology* / Q000601:surgery; D013505:Digestive System Surgical Procedures; D004801:Eosine Yellowish-(YS); D005456:Fluorescent Dyes; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007422:Intestines / Q000473:pathology* / Q000601:surgery; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents; D005456:Fluorescent Dyes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin