title: Increased migration of a human glioma cell line after in vitro CyberKnife irradiation.
abstract: A human glioblastoma multiforme cell line (U87) and its derived-spheroids were irradiated either using a conventional irradiation (CIR) or a CK-like irradiation (IIR) in which the 8 Gy was delivered intermittently over a period of 40 minutes. The ability of glioma cells to migrate into a matrigel matrix was evaluated on days 1-8 from irradiation. Irradiation with CK-driven IIR significantly increased the invasion potential of U87 cells in a matrigel-based assay. In contrast to CIR, IIR was associated with increased levels of TGF-β at four days (Real time PCR), β1-integrin at 4-5 days (real-time PCR and western blot) and no elevation in phosphorylated AKT at days 4 and 5 (western blot). Our data suggests that glioma cell invasion as well as elevations of TGF-β and β1-integrin are associated with IIR and not CIR.
authors.affiliation: Laboratory of Clinical Investigation, Fondazione IRCCS Istituto Neurologico C. Besta, Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Canazza
authors.forename: Chiara
authors.initials: C
authors.lastname: Calatozzolo
authors.forename: Luisa
authors.initials: L
authors.lastname: Fumagalli
authors.forename: Achille
authors.initials: A
authors.lastname: Bergantin
authors.forename: Francesco
authors.initials: F
authors.lastname: Ghielmetti
authors.forename: Laura
authors.initials: L
authors.lastname: Fariselli
authors.forename: Danilo
authors.initials: D
authors.lastname: Croci
authors.forename: Andrea
authors.initials: A
authors.lastname: Salmaggi
authors.forename: Emilio
authors.initials: E
authors.lastname: Ciusani
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects; D016543:Central Nervous System Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D003094:Collagen; D004338:Drug Combinations; D005909:Glioblastoma / Q000473:pathology / Q000532:radiotherapy; D005910:Glioma / Q000378:metabolism / Q000473:pathology* / Q000532:radiotherapy*; D006801:Humans; D019012:Integrin beta1 / Q000378:metabolism; D007797:Laminin; D009361:Neoplasm Invasiveness; D051058:Oncogene Protein v-akt / Q000378:metabolism; D010766:Phosphorylation / Q000528:radiation effects; D011509:Proteoglycans; D016634:Radiosurgery / Q000295:instrumentation; D016212:Transforming Growth Factor beta / Q000378:metabolism; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D004338:Drug Combinations; D019012:Integrin beta1; D007797:Laminin; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; C059485:matrigel; D003094:Collagen; D051058:Oncogene Protein v-akt