title: Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: the importance of codon optimization and clone selection.
abstract: In the last 15 years, 80% of all recombinant proteins reported in the literature were produced in the bacterium, Escherichia coli, or the yeast, Pichia pastoris. Nonetheless, developing effective general strategies for producing recombinant eukaryotic membrane proteins in these organisms remains a particular challenge. Using a validated screening procedure together with accurate yield quantitation, we therefore wished to establish the critical steps contributing to high yields of recombinant eukaryotic membrane protein in P. pastoris. Whilst the use of fusion partners to generate chimeric constructs and directed mutagenesis have previously been shown to be effective in bacterial hosts, we conclude that this approach is not transferable to yeast. Rather, codon optimization and the preparation and selection of high-yielding P. pastoris clones are effective strategies for maximizing yields of human aquaporins.
authors.affiliation: Department of Chemistry/Biochemistry, University of Gothenburg, Göteborg, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Öberg
authors.forename: Jennie
authors.initials: J
authors.lastname: Sjöhamn
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Conner
authors.forename: Roslyn M
authors.initials: RM
authors.lastname: Bill
authors.forename: Kristina
authors.initials: K
authors.lastname: Hedfalk
mesh_terms: D020346:Aquaporins / Q000235:genetics / Q000378:metabolism; D003062:Codon / Q000235:genetics*; D018274:Electroporation; D006801:Humans; D018021:Lithium Chloride; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D020346:Aquaporins; D003062:Codon; D011994:Recombinant Proteins; D018021:Lithium Chloride