title: [Effect of regulation of Y-box protein 1 by RNA interference on the doxorubicin induced mdr1 gene expression in K562 cells].
abstract: OBJECTIVE
  To investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells.
  
  
  METHODS
  K562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected.
  
  
  RESULTS
  The mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells.
  
  
  CONCLUSIONS
  Doxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.
authors.affiliation: Department of Central Laboratory, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Shen
authors.forename: Wen-Lin
authors.initials: WL
authors.lastname: Xu
authors.forename: Qiao-Yun
authors.initials: QY
authors.lastname: Chen
authors.forename: Fa-Chun
authors.initials: FC
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Fei
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D020014:K562 Cells; D021381:Protein Transport; D034622:RNA Interference; D034741:RNA, Small Interfering; D051840:Y-Box-Binding Protein 1 / Q000235:genetics*
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D034741:RNA, Small Interfering; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D004317:Doxorubicin