title: Exploiting the interactions of PNA-DNA films with Ni2+ ions: detection of nucleobase mismatches and electrochemical genotyping of the single-nucleotide mismatch in apoE 4 related to Alzheimer's disease.
abstract: The presence of Ni(2+) enables us to distinguish the presence of single-nucleotide mismatches in PNA (peptide nucleic acids)-DNA films on gold electrodes by electrochemical impedance spectroscopy (EIS). With the help of a modified Randles' equivalent circuit, differences in the charge transfer resistance (ΔR(CT)) before and after the addition of Ni(2+) are a diagnostic measure for the presence of single-nucleotide mismatch. The approach works under real-life conditions with concentrations of the DNA target strand down to 10 fM, and a PNA capture probe is used to genotype the single-nucleotide mismatch in apoE 4 related to Alzheimer's disease (AD).
authors.affiliation: Department of Chemistry, Beijing Normal University, Beijing, China.
authors.forename: Kaijuan
authors.initials: K
authors.lastname: Guo
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Li
authors.forename: Heinz-Bernhard
authors.initials: HB
authors.lastname: Kraatz
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000235:genetics*; D053327:Apolipoprotein E4 / Q000235:genetics*; D020137:Base Pair Mismatch; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004252:DNA Mutational Analysis / Q000379:methods*; D058266:Dielectric Spectroscopy; D006046:Gold / Q000737:chemistry; D006801:Humans; D009532:Nickel / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D053327:Apolipoprotein E4; D020135:Peptide Nucleic Acids; D006046:Gold; D009532:Nickel; D004247:DNA