title: Diazirine based photoaffinity labeling.
abstract: Diazirines are among the smallest photoreactive groups that form a reactive carbene upon light irradiation. This feature has been widely utilized in photoaffinity labeling to study ligand-receptor, ligand-enzyme and protein-protein interactions, and in the isolation and identification of unknown proteins. This review summarizes recent advances in the use of diazirines in photoaffinity labeling.
authors.affiliation: Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel.
authors.forename: Luba
authors.initials: L
authors.lastname: Dubinsky
authors.forename: Bastiaan P
authors.initials: BP
authors.lastname: Krom
authors.forename: Michael M
authors.initials: MM
authors.lastname: Meijler
mesh_terms: D003978:Diazomethane / Q000138:chemical synthesis / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D009696:Nucleic Acids / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000737:chemistry; D019920:Photoaffinity Labels / Q000737:chemistry*; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D004798:Enzymes; D009696:Nucleic Acids; D004364:Pharmaceutical Preparations; D019920:Photoaffinity Labels; D011506:Proteins; D003978:Diazomethane