title: Lentivectors are efficient tools to manipulate the dendritic cell cytoskeleton.
abstract: Dendritic cells (DC) are key cells of the innate immune system required to prime adaptive immunity. Central DC functions including antigen uptake and presentation and DC migration are critically dependent on dynamic cytoskeletal reorganisation, the regulation of which remains poorly understood. Cytoskeletal studies are complicated by the fact that DC cytoarchitecture is altered considerably by maturation stimuli, including many tools employed for biological manipulation. Lentiviral vectors, capable of transducing non-dividing cells such as DC, hold promise both for experimental and therapeutic manipulation of DC gene and protein expression but controversy remains about their effect on DC maturation. Here, we have examined the potential of lentiviral vectors as tools for gene delivery to monocyte derived human DC with preservation of immature DC cytoskeletal structure and function. We show that vesicular stomatitis virus G glycoprotein (VSVG)-pseudotyped lentivectors are most efficient at transducing immature DC and their precursor monocytes. Even at high multiplicities of infection transduced DC retained an immature cytoskeletal phenotype, with no significant alteration of migration, antigen uptake or T-cell stimulation capacities. Furthermore, lentivectors did not alter subsequent functional maturation of the DC cytoskeleton in response to lipopolysaccharide exposure. Together our data show that VSVG-psudotyped lentiviral vectors are an effective tool for gene manipulation in human DC with preservation of functional immaturity and plasticity, making them ideal for studies of the DC cytoskeleton.
authors.affiliation: Molecular Immunology Unit, Wolfson Centre for Gene Therapy of Childhood Disease, UCL Institute of Child Health, Great Ormond Street Hospital for Children NHS Trust, London, United Kingdom.
authors.forename: Joao
authors.initials: J
authors.lastname: Metelo
authors.forename: Natalie
authors.initials: N
authors.lastname: Ward
authors.forename: Adrian J
authors.initials: AJ
authors.lastname: Thrasher
authors.forename: Siobhan O
authors.initials: SO
authors.lastname: Burns
mesh_terms: D003599:Cytoskeleton / Q000502:physiology / Q000821:virology*; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism / Q000821:virology*; D005822:Genetic Vectors; D006801:Humans; D016130:Immunophenotyping; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014161:Transduction, Genetic