title: Assessment of an ad hoc procedure for isolation and characterization of human albuminome.
abstract: The dynamic range of plasma protein abundance, ranging from milligrams to picograms per milliliter, makes characterization of this proteome nearly impossible with current analytical methods. Plasma preprocessing by high-abundance protein depletion may concomitantly remove important diagnostic information. This article describes an original chromatographic procedure to isolate proteins bound to human serum albumin (HSA). Using HSA as an "affinity agent", we significantly improved the detection and identification of HSA ligands by two-dimensional liquid chromatography tandem mass spectrometry (2D LC-MS/MS). Some of the characterized species were not previously reported in published blood databases. Albumin-binding proteins may be classified as belonging to several putative functional categories and span a wide variety of predicted physiological functions.
authors.affiliation: Department of Experimental and Clinical Medicine, Magna Graecia University, School of Medicine, Germaneto University Campus, 88100 Catanzaro, Italy.
authors.forename: Domenica
authors.initials: D
authors.lastname: Scumaci
authors.forename: Marco
authors.initials: M
authors.lastname: Gaspari
authors.forename: Milena
authors.initials: M
authors.lastname: Saccomanno
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Argirò
authors.forename: Barbara
authors.initials: B
authors.lastname: Quaresima
authors.forename: Concetta M
authors.initials: CM
authors.lastname: Faniello
authors.forename: Pietrantonio
authors.initials: P
authors.lastname: Ricci
authors.forename: Francesco
authors.initials: F
authors.lastname: Costanzo
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cuda
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D020543:Proteome; D012709:Serum Albumin