title: Anandamide enhances expression of heat shock proteins Hsp70 and Hsp25 in rat lungs.
abstract: Anandamide (AEA), an endogenous cannabinoid and vanilloid receptor ligand, possesses anti-inflammatory properties. Transport of AEA through cytoplasm is facilitated by heat shock protein (HSP) Hsp70, which enhances the rate of cellular AEA uptake, possibly via direct interactions. In lungs, increased HSP expression is an endogenous, protective mechanism against acute lung inflammation. We hypothesised that AEA could enhance the expression of cytoprotective Hsp70 and Hsp25. Anaesthetised rats were injected intravenously with 1mg/kg AEA or saline. Lungs were removed 2 and 24 h after injection for evaluation of HSP expression. Hsp70 and Hsp25 expression in lungs was evaluated by immunohistochemistry. The relative levels of these HSPs in lung sections were determined through optical density measurements and western blotting of lung homogenates. Western blot and immunohistochemistry analyses indicated that expression of both proteins was significantly higher in AEA-injected animals than in control animals 2 and 24 h after treatment. AEA administration enhanced Hsp70 and Hsp25 expression in lungs. Therefore, AEA-HSP interactions could be involved in mechanisms protecting against lung inflammation, indicating a possible use of AEA as a treatment for lung inflammation.
authors.affiliation: Mossakowski Medical Research Centre PAS, Laboratory of Respiratory Reflexes, Poland. kopczynb@cmdik.pan.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Kopczyńska
authors.forename: Dorota
authors.initials: D
authors.lastname: Sulejczak
authors.forename: Marlena
authors.initials: M
authors.lastname: Wełniak-Kamińska
authors.forename: Aleksander
authors.initials: A
authors.lastname: Gietka
authors.forename: Paweł
authors.initials: P
authors.lastname: Grieb
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000378:metabolism / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D019610:Cytoprotection / Q000187:drug effects; D063388:Endocannabinoids; D005786:Gene Expression Regulation / Q000187:drug effects*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D053284:Polyunsaturated Alkamides / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D001095:Arachidonic Acids; D063388:Endocannabinoids; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D053284:Polyunsaturated Alkamides; C078814:anandamide