title: Isolation of yellow fever virus (YFV) from naturally infected Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009.
abstract: After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus.
authors.affiliation: Núcleo de Doenças de Transmissão Vetorial, Instituto Adolfo Lutz, Secretaria de Estado da Saúde de São Paulo, São Paulo, SP, Brasil. renatoefabi@gmail.com
authors.forename: Renato Pereira de
authors.initials: RP
authors.lastname: Souza
authors.forename: Selma
authors.initials: S
authors.lastname: Petrella
authors.forename: Terezinha Lisieux Moraes
authors.initials: TL
authors.lastname: Coimbra
authors.forename: Adriana Yurika
authors.initials: AY
authors.lastname: Maeda
authors.forename: Iray Maria
authors.initials: IM
authors.lastname: Rocco
authors.forename: Ivani
authors.initials: I
authors.lastname: Bisordi
authors.forename: Vivian Regina
authors.initials: VR
authors.lastname: Silveira
authors.forename: Luiz Eloy
authors.initials: LE
authors.lastname: Pereira
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Sarai Joaquim Dos Santos
authors.initials: SJ
authors.lastname: Silva
authors.forename: Fernanda Gisele
authors.initials: FG
authors.lastname: Silva
authors.forename: Felipe Scassi
authors.initials: FS
authors.lastname: Salvador
authors.forename: Rosa Maria
authors.initials: RM
authors.lastname: Tubaki
authors.forename: Regiane Tironi
authors.initials: RT
authors.lastname: Menezes
authors.forename: Mariza
authors.initials: M
authors.lastname: Pereira
authors.forename: Eduardo Sterlino
authors.initials: ES
authors.lastname: Bergo
authors.forename: Roberto Colozza
authors.initials: RC
authors.lastname: Hoffmann
authors.forename: Roberta Maria Fernandes
authors.initials: RM
authors.lastname: Spinola
authors.forename: Cílea Hatsumi
authors.initials: CH
authors.lastname: Tengan
authors.forename: Melissa Mascheratti
authors.initials: MM
authors.lastname: Siciliano
mesh_terms: D000508:Alouatta; D000818:Animals; D001938:Brazil; D009033:Culicidae / Q000145:classification / Q000821:virology*; D007303:Insect Vectors / Q000145:classification / Q000821:virology*; D051379:Mice; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015004:Yellow Fever / Q000635:transmission; D015005:Yellow fever virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral