title: Effect of neurotrophin-4 on endoplasmic reticulum stress-related neuronal apoptosis in diabetic and high glucose exposed rat retinas.
abstract: The purpose of this study was to investigate the effect of NT-4 on the endoplasmic reticulum (ER) stress-related apoptosis of retinal neurons of isolated retinas. The retinas were isolated from normal and diabetic rats, and the normal retinas were exposed to high glucose (HG). Our results showed that the number of TUNEL-positive, and PERK- and CHOP-positive cells was significantly higher in diabetic and HG exposed retinas than in normal retinas. In diabetic and HG exposed retinas supplemented with NT-4, the number of TUNEL-positive, and PERK- and CHOP-positive cells was significantly lower than in retinas without NT-4. The neuroprotective effect of NT-4 on retinas cultured under diabetic stress was correlated with the suppression in the expression of PERK and CHOP, ER stress-related factors.
authors.affiliation: Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan. Tarii@aol.com
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Oshitari
authors.forename: Natsuyo
authors.initials: N
authors.lastname: Yoshida-Hata
authors.forename: Shuichi
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D048909:Diabetes Complications / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000502:physiology*; D005947:Glucose / Q000633:toxicity*; D008297:Male; D009414:Nerve Growth Factors / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D055351:Retinal Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D051742:Transcription Factor CHOP / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019892:eIF-2 Kinase / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D005947:Glucose; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D051742:Transcription Factor CHOP; D019892:eIF-2 Kinase; C497251:Ddit3 protein, rat; C073922:neurotrophin 4