title: Neuroprotective effects of Buyang Huanwu decoction against hydrogen peroxide induced oxidative injury in Schwann cells.
abstract: BACKGROUND
  Buyang Huanwu decoction (BYHWD) is a traditional Chinese medicine and can be used to promote peripheral nerve regeneration. However the regenerative mechanism of BYHWD remains unclear. The objective of this study was to investigate the protective mechanisms of BYHWD in Schwann cells damaged by hydrogen peroxide (H(2)O(2)).
  
  
  METHODS
  Schwann cells which were derived from neonatal sciatic nerves of rats were used in subsequent experiments. Schwann cells were injured by various concentrations of H(2)O(2) (0.25, 0.5 and 1mM final concentration). BYHWD (600 μg/ml final concentration) was added to the medium either simultaneously or 1h later after the addition of H(2)O(2). Subsequently, methyl thiazolyl tetrazolium (MTT) assay was performed. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels were also examined after 12h. The expression of Caspase 3 and the concentration of intercellular Ca(2+) ([Ca(2+)]i) were also determined.
  
  
  RESULTS
  Among three concentrations of H(2)O(2), 0.5mM H(2)O(2) induced Schwann cells swelled and neuritis disappeared after 12h. In the presence of BYHWD, MTT assay showed that more cells were viable in comparison with the H(2)O(2) injury group. Moreover, the addition of BYHWD has also increased the SOD activity with decreased in MDA level. Furthermore, the concentration of [Ca(2+)]i and expression of Caspase 3 were decreased with the addition of BYHWD in culture.
  
  
  CONCLUSIONS
  Our results revealed that BYHWD protected Schwann cells from oxidative injury. The mechanism of BYHWD promoting neural regeneration possibly associated with its anti-oxidative activity.
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology and Department of Anatomy, PR China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
authors.forename: Sanli
authors.initials: S
authors.lastname: Xing
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Feng
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Haiman
authors.initials: H
authors.lastname: Dong
authors.forename: Lihua
authors.initials: L
authors.lastname: Bao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanlai
authors.initials: Y
authors.lastname: Hu
authors.forename: Guibao
authors.initials: G
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Li
authors.forename: Jinhao
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D016877:Oxidants; C460102:buyang huanwu; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; C505410:Casp3 protein, rat; D053148:Caspase 3; D002118:Calcium