title: Transcription profiles of LPS-stimulated THP-1 monocytes and macrophages: a tool to study inflammation modulating effects of food-derived compounds.
abstract: An assay was developed to study inflammation-related immune responses of food compounds on monocytes and macrophages derived from THP-1 cell line. First strategy focused on the effects after stimulation with either lipopolysaccharide (LPS) or Concanavalin A (ConA). Gene expression kinetics of inflammation-related cytokines (IL-1β, IL-6, IL-8, IL-10 and TNF-α), inflammation-related enzymes (iNOS and COX-2), and transcription factors (NF-κB, AP-1 and SP-1) were analyzed using RT-PCR. Time dependent cytokine secretion was investigated to study the inflammation-related responses at protein level. LPS stimulation induced inflammation-related cytokine, COX-2 and NF-κB genes of THP-1 monocytes and THP-1 macrophages with the maximum up-regulation at 3 and 6 h, respectively. These time points, were subsequently selected to investigate inflammation modulating activity of three well known immuno-modulating food-derived compounds; quercetin, citrus pectin and barley glucan. Co-stimulation of LPS with either quercetin, citrus pectin, or barley glucan in THP-1 monocytes and macrophages showed different immuno-modulatory activity of these compounds. Therefore, we propose that simultaneously exposing THP-1 cells to LPS and food compounds, combined with gene expression response analysis are a promising in vitro screening tool to select, in a limited time frame, food compounds for inflammation modulating effects.
authors.affiliation: Cell Biology and Immunology Group, Wageningen University and Research Centre, Marijkeweg 40, P.O. Box 338, 6700 AH, Wageningen, The Netherlands.
authors.forename: Wasaporn
authors.initials: W
authors.lastname: Chanput
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Mes
authors.forename: Robert A M
authors.initials: RA
authors.lastname: Vreeburg
authors.forename: Huub F J
authors.initials: HF
authors.lastname: Savelkoul
authors.forename: Harry J
authors.initials: HJ
authors.lastname: Wichers
mesh_terms: D045744:Cell Line, Tumor; D002957:Citrus / Q000737:chemistry; D003208:Concanavalin A / Q000494:pharmacology; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D005936:Glucans / Q000494:pharmacology*; D001467:Hordeum / Q000737:chemistry; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000276:immunology; D010368:Pectins / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D005936:Glucans; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D008934:Mitogens; D014157:Transcription Factors; D003208:Concanavalin A; D010368:Pectins; D011794:Quercetin; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human