title: Strategy for modular tagged high-throughput amplicon sequencing.
abstract: The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.
authors.affiliation: CSIRO Preventative Health National Research Flagship and Livestock Industries, Queensland Bioscience Precinct, St. Lucia, QLD 4067, Australia.
authors.forename: Daniel Aguirre
authors.initials: DA
authors.lastname: de Cárcer
authors.forename: Stuart E
authors.initials: SE
authors.lastname: Denman
authors.forename: Chris
authors.initials: C
authors.lastname: McSweeney
authors.forename: Mark
authors.initials: M
authors.lastname: Morrison
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D056186:Metagenomics / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers