title: A novel anti-lymphoma protein RE26 from Rozites emodensis (Berk.) Moser.
abstract: A novel antitumor protein, designated RE26, with anti-lymphoma activity was purified from a Tris-HCl buffer extract of Rozites emodensis (Berk.) Moser by three successive steps of ion exchange chromatography. SDS-PAGE and gel filtration chromatography revealed that RE26 is a monomeric protein of 26 kDa, and isoelectrofocusing assay indicated its isoelectric point of 4.3-4.4. RE26 has high stability over a wide pH range (pH 3-11) but is sensitive to temperature and only stable under 40 °C. Partial amino acid sequences of two RE26 peptide fragments were determined by Edman degradation as GLEEEETLLLLFFPP and GTEQE. The half-maximal inhibitory concentration (IC₅₀) of RE26 against tested lymphoma cell lines was around 4 μg/ml. In vitro experiments showed that RE26 could specifically bind to lymphoma cells; activate the caspases, including caspases 3, 8, and 9 in host cells; and induce apoptosis. Experiments in nude mice indicated local RE26 injection adjacent to tumor site could inhibit lymphoma formation.
authors.affiliation: Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610064, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Gong
authors.forename: Minjie
authors.initials: M
authors.lastname: Xia
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiao
authors.initials: J
authors.lastname: Gu
authors.forename: Xuyang
authors.initials: X
authors.lastname: Liu
authors.forename: Jingqiu
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis; D002460:Cell Line; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020128:Inhibitory Concentration 50; D008223:Lymphoma / Q000188:drug therapy; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins