title: Human feeder cell line for derivation and culture of hESc/hiPSc.
abstract: We have generated a human feeder cell line from early second trimester Placental Stromal Fibroblasts (ihPSF) stably over-expressing the polycomb protein BMI-1. These feeder cells retain the ability to maintain human Embryonic Stem cells (hESc) over long-term culture whereas hTERT or BMI-1/hTERT immortalised feeder cell lines do not. ihPSFs were able to support the derivation of a new hESc line in near xenofree (free of non-human animal components) conditions and support continued culture of newly derived hESc and human induced Pluripotent Stem (hiPS) cell lines in complete xenofree conditions necessary for clinical use.
authors.affiliation: North West Embryonic Stem Cell Centre, Faculty of Life Sciences, Core Technology Facility, University of Manchester, 46 Grafton Street, Manchester, M13 9NT, UK. t.mckay@qmul.ac.uk
authors.forename: Tristan R
authors.initials: TR
authors.lastname: McKay
authors.forename: Maria V
authors.initials: MV
authors.lastname: Camarasa
authors.forename: Banu
authors.initials: B
authors.lastname: Iskender
authors.forename: Jinpei
authors.initials: J
authors.lastname: Ye
authors.forename: Nicola
authors.initials: N
authors.lastname: Bates
authors.forename: Duncan
authors.initials: D
authors.lastname: Miller
authors.forename: Jayne C
authors.initials: JC
authors.lastname: Fitzsimmons
authors.forename: Daniel
authors.initials: D
authors.lastname: Foxler
authors.forename: Maureen
authors.initials: M
authors.lastname: Mee
authors.forename: Tyson V
authors.initials: TV
authors.lastname: Sharp
authors.forename: John
authors.initials: J
authors.lastname: Aplin
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Brison
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Kimber
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling / Q000379:methods; D039904:Pluripotent Stem Cells / Q000166:cytology*