title: Control of a nanoscopic-to-macroscopic transition: modulated phases in four-component DSPC/DOPC/POPC/Chol giant unilamellar vesicles.
abstract: We have found modulated phase morphology in a particular region of composition within the liquid-ordered + liquid-disordered coexistence region in the four-component lipid bilayer mixture DSPC/DOPC/POPC/Chol. By controlling lipid composition, we could see distinct types of modulated liquid-liquid phase morphologies, including linear, irregular, and angular features in giant unilamellar vesicles. We used a combination of confocal, two-photon, wide-field fluorescence, and differential interference contrast microscopies, and used stringent controls to minimize light-induced artifacts. These studies establish that both the size and morphology of membrane rafts can be controlled by the concentration and the type of low-melting lipid in mixtures with cholesterol and a high-melting lipid.
authors.forename: Tatyana M
authors.initials: TM
authors.lastname: Konyakhina
authors.forename: Shih Lin
authors.initials: SL
authors.lastname: Goh
authors.forename: Jonathan
authors.initials: J
authors.lastname: Amazon
authors.forename: Frederick A
authors.initials: FA
authors.lastname: Heberle
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: Gerald W
authors.initials: GW
authors.lastname: Feigenson
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry*; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry*; D013696:Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; D002784:Cholesterol; C010942:1,2-distearoyllecithin; C017251:1,2-oleoylphosphatidylcholine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine