title: Fibroblast growth factor-binding protein facilitates the growth and migration of skin-derived precursors.
abstract: BACKGROUND
  Fibroblast growth factors (FGFs) are important regulators of cell proliferation, migration, and differentiation during wound healing. FGF-binding protein (FGF-BP) plays a critical role in activating FGFs by releasing them from the extracellular matrix. Although previous studies have demonstrated a pivotal role for FGF-BP in wound healing and angiogenesis, little is known about the biologic effects of FGF-BP on skin stem cells that contribute to wound healing.
  
  
  OBJECTIVE
  To investigate the effects of FGF-BP on the growth and migration of skin-derived precursors (SKPs).
  
  
  METHODS
  FGF-BP was titrated to determine the optimal concentration that maximally stimulated cell proliferation. Cellular phenotype and telomerase activity were compared in the presence and absence of FGF-BP. The effect of FGF-BP on cell migration was observed by intravenously transplanting SKPs to adult mice.
  
  
  RESULTS
  Cell proliferation was maximally stimulated by FGF-BP at a concentration of 10 ng/mL without changing the intrinsic characteristics of SKPs. Low levels of telomerase activity were detected, and FGF-BP decreased the rate at which telomerase activity was downregulated. In vivo, FGF-BP remarkably enhanced the migration of SKPs to skin lesion sites.
  
  
  CONCLUSIONS
  FGF-BP exerts a positive effect on the growth and migration of SKPs, suggesting a potential role for SKPs in wound healing.
authors.affiliation: Department of Burns and Emergency Surgery, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Shao-Hai
authors.initials: SH
authors.lastname: Qi
authors.forename: Bin
authors.initials: B
authors.lastname: Shu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Ju-Lin
authors.initials: JL
authors.lastname: Xie
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Xu
authors.forename: Xu-Sheng
authors.initials: XS
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005434:Flow Cytometry; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D039902:Multipotent Stem Cells / Q000187:drug effects* / Q000637:transplantation; D010641:Phenotype; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D002352:Carrier Proteins; C489453:Fgfbp1 protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016222:Fibroblast Growth Factor 2; D019098:Telomerase