title: Role of the COX-independent pathways in the ulcer-healing action of epigallocatechin gallate.
abstract: The modulation of the cyclooxygenase-independent pathway by the green tea-derived polyphenol, epigallocatechin gallate (EGCG) during its healing action against indomethacin (IND)-induced stomach ulceration in mice was investigated. On the 3rd day of its administration, IND (18 mg kg(-1)) induced maximum stomach ulceration which was associated with increased myeloperoxidase (MPO) activity (2.1-fold, p < 0.001), and inducible nitric oxide synthase (iNOS) expression (2.5-fold, p < 0.001), along with augmented levels of serum nitrite (1.3-fold, p < 0.001), selectins and cell adhesion molecules (CAMs), as well as reduced endothelial nitric oxide synthase (eNOS) expression (53%, p < 0.001). Treatment with EGCG (2 mg kg(-1)) and omeprazole (3 mg kg(-1)) for 3 days reversed these parameters, and provided excellent (76-77%) ulcer healing.
authors.affiliation: IPGME & R, 244B, Acharya Jagadish Chandra Bose Road, Kolkata 700 020, India.
authors.forename: Biplab
authors.initials: B
authors.lastname: Adhikary
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Yadav
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Bandyopadhyay
authors.forename: Subrata
authors.initials: S
authors.lastname: Chattopadhyay
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D005786:Gene Expression Regulation; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000097:blood / Q000235:genetics; D009573:Nitrites / Q000097:blood; D009853:Omeprazole / Q000494:pharmacology; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D019087:Selectins / Q000097:blood; D015398:Signal Transduction; D013276:Stomach Ulcer / Q000188:drug therapy*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014456:Ulcer / Q000188:drug therapy*; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood
chemical_list: D000897:Anti-Ulcer Agents; D015850:Interleukin-6; D009573:Nitrites; D019087:Selectins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D015847:Interleukin-4; D002392:Catechin; C045651:epigallocatechin gallate; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D009853:Omeprazole