title: Roseivivax isoporae sp. nov., isolated from a reef-building coral, and emended description of the genus Roseivivax.
abstract: A bacterial strain, isolated from a sample of reef-building coral (Isopora palifera) collected off the coast of southern Taiwan, was characterized using a polyphasic taxonomic approach. The strain, designated sw-2(T), was Gram-staining-negative, aerobic, rod-shaped and motile, with subpolar flagella, and formed greyish pink colonies. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain sw-2(T) was most closely related to Roseivivax halodurans Och 239(T) (97.4 % sequence similarity) and Roseivivax halotolerans Och 210(T) (96.4 %). The novel strain did not require NaCl for growth and exhibited optimal growth at 35-40 °C, at pH 7.5-8.0 and with 3-7 % (w/v) NaCl. It produced bacteriochlorophyll a under aerobic conditions. Summed feature 8 (C(18:1)ω7c and/or C(18:1)ω6c; 63.7 %) predominated in the cellular fatty acid profile. The novel strain's major respiratory quinone was ubiquinone Q-10 and its genomic DNA G+C content was 68.8 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidyldimethylethanolamine, sulfo-quinovosyl diacylglycerol and three uncharacterized phospholipids. The level of DNA-DNA relatedness between strain sw-2(T) and Roseivivax halodurans Och 239(T) was only 15.0 %. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of the novel strain from all established species of the genus Roseivivax. Based on the genotypic, phenotypic and chemotaxonomic data, strain sw-2(T) represents a novel species in the genus Roseivivax, for which the name Roseivivax isoporae sp. nov. is proposed. The type strain is sw-2(T) ( = LMG 25204(T) = BCRC 17966(T)).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, Kaohsiung, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Research Center for Biodiversity, Academia Sinica, Nangang 115, Taipei, Taiwan, ROC.
authors.forename: Chaolun Allen
authors.initials: CA
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, Tajen University, Yanpu, Pingtung 907, Taiwan, ROC.
authors.forename: Jih-Terng
authors.initials: JT
authors.lastname: Wang
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride