title: Euthanization methods influence cytokine mRNA expression levels in age 0 year Oncorhynchus mykiss.
abstract: Significant differences in cytokine transcription were found between Oncorhynchus mykiss euthanized using the pharmacological agents MS-222 v. benzocaine and also when contrasting death induced by carbon dioxide asphyxiation v. physical methods (cervical dislocation). This study highlights the need to consider the potentially confounding effect of euthanization method on gene expression data.
authors.affiliation: Center for Watershed Sciences, One Shields Avenue, University of California, Davis, CA 95616, USA. lbowen@ucdavis.edu
authors.forename: L
authors.initials: L
authors.lastname: Bowen
authors.forename: J D
authors.initials: JD
authors.lastname: Kiernan
authors.forename: K
authors.initials: K
authors.lastname: Eder
authors.forename: I
authors.initials: I
authors.lastname: Werner
authors.forename: C M
authors.initials: CM
authors.lastname: Leutenegger
authors.forename: M A
authors.initials: MA
authors.lastname: Adkison
authors.forename: R P
authors.initials: RP
authors.lastname: Hedrick
authors.forename: M L
authors.initials: ML
authors.lastname: Johnson
mesh_terms: D062365:Aminobenzoates / Q000494:pharmacology; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001237:Asphyxia / Q000378:metabolism; D001566:Benzocaine / Q000494:pharmacology; D002245:Carbon Dioxide / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism*; D037901:Euthanasia, Animal / Q000379:methods*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D062365:Aminobenzoates; D000777:Anesthetics; D016207:Cytokines; D012333:RNA, Messenger; C003636:tricaine; D002245:Carbon Dioxide; D001566:Benzocaine