title: Nucleolar proteins with altered expression in leukemic cell lines.
abstract: The function of the nucleolus is intimately connected to cell proliferation, division and growth. Many cancer cells have enlarged nucleoli, and several nucleolar proteins have been linked to tumorigenesis. In order to find proteins whose expression is altered in the nucleoli of leukemic cells, we carried out two-dimensional difference gel electrophoresis (2-D DIGE) analyses. Prohibitin (PHB) and TAR-DNA-binding protein-43 (TDP-43) were strongly expressed in the nucleoli of the pre-B-ALL cell line MHH-CALL3. Our results demonstrate that leukemic cells have differences in their nucleolar protein composition, and suggest that it may be possible to exploit these differences in identification of leukemia subtypes.
authors.affiliation: Paediatric Research Centre, University of Tampere Medical School and Tampere University Hospital, Tampere, Finland. kaisa.teittinen@uta.fi
authors.forename: Kaisa J
authors.initials: KJ
authors.lastname: Teittinen
authors.forename: Pauliina
authors.initials: P
authors.lastname: Kärkkäinen
authors.forename: Johanna
authors.initials: J
authors.lastname: Salonen
authors.forename: Gunilla
authors.initials: G
authors.lastname: Rönnholm
authors.forename: Hanna
authors.initials: H
authors.lastname: Korkeamäki
authors.forename: Mauno
authors.initials: M
authors.lastname: Vihinen
authors.forename: Nisse
authors.initials: N
authors.lastname: Kalkkinen
authors.forename: Olli
authors.initials: O
authors.lastname: Lohi
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002466:Cell Nucleolus / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000378:metabolism*; D000091402:Prohibitins; D012097:Repressor Proteins / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000717328:PHB protein, human; D000091402:Prohibitins; D012097:Repressor Proteins