title: Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP.
abstract: Organophosphate and -phosphonates and their thio derivatives are often used in agroindustry as herbicides and insecticides, but their potential off-targets in the plant are poorly investigated. Here, we use competitive activity-based protein profiling (ABPP) of serine hydrolases (SHs) to detect targets of these agrochemicals and other compounds in Arabidopsis thaliana. Using broad-range and specific probes, and by overexpression of various SHs in planta, we are able to confirm eight SH-compound interactions, including selective inhibition of carboxylesterase CXE12, prolyloligopeptidase, methylesterase MES2 and tripeptidyl peptidase TPP2. These observations can be used for the design of novel probes and selective inhibitors and may help to assess physiological effects of agrochemicals on crop plants.
authors.affiliation: The Plant Chemetics Laboratory, Chemical Genomics Centre of the Max Planck Society, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, D-50829 Cologne, Germany.
authors.forename: Farnusch
authors.initials: F
authors.lastname: Kaschani
authors.forename: Sabrina
authors.initials: S
authors.lastname: Nickel
authors.forename: Bikram
authors.initials: B
authors.lastname: Pandey
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Cravatt
authors.forename: Markus
authors.initials: M
authors.lastname: Kaiser
authors.forename: Renier A L
authors.initials: RA
authors.lastname: van der Hoorn
mesh_terms: D016573:Agrochemicals / Q000737:chemistry* / Q000494:pharmacology; D017360:Arabidopsis / Q000201:enzymology; D006540:Herbicides / Q000737:chemistry; D007306:Insecticides / Q000737:chemistry; D010755:Organophosphates / Q000737:chemistry; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D040901:Proteomics; D057057:Serine Proteases / Q000737:chemistry* / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*
chemical_list: D016573:Agrochemicals; D006540:Herbicides; D007306:Insecticides; D010755:Organophosphates; D010940:Plant Proteins; D015842:Serine Proteinase Inhibitors; D057057:Serine Proteases