title: Osteoblast differentiation of amniotic fluid-derived stem cells irradiated with visible light.
abstract: The effect of visible light irradiation on the expression of pluripotent genes (Oct-4, Sox2, and Nanog) in amniotic fluid-derived stem cells (AFSCs) and on the osteogenic differentiation ability of AFSCs was investigated using light-emitting diodes (LEDs) at 0-2 mW/cm(2) in various wavelengths: [blue (470 nm), green (525 nm), yellow (600 nm), and red (630 nm)]. Pluripotent gene expression in AFSCs was up-regulated by visible light irradiation from a LED for more than 6 h. Green light irradiation of AFSCs up-regulated the expression of pluripotent genes more significantly than irradiation with other light. The osteogenic differentiation of AFSCs was facilitated by green and blue light irradiation. Facilitated differentiation into osteogenic cells by visible light irradiation was not mediated by reactive oxygen species (ROS); alkaline phosphatase activity (a marker of early osteogenic differentiation) and gene expression of osteopontin (a marker of late osteogenic differentiation) did not change significantly between AFSCs in differentiation medium with or without a ROS scavenger (vitamin C). The mitogen-activated protein kinase/extracellular signal-regulated protein kinase pathway, as well as other unknown signaling pathways, may be responsible for the activation of signaling pathways that facilitate the differentiation of AFSCs into osteogenic cells on light irradiation.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan, Taiwan. higuchi@ncu.edu.tw
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.forename: Po-Yen
authors.initials: PY
authors.lastname: Shen
authors.forename: Jun-Kai
authors.initials: JK
authors.lastname: Zhao
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Ling
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Han-Chow
authors.initials: HC
authors.lastname: Wang
authors.forename: Jun-Tang
authors.initials: JT
authors.lastname: Bing
authors.forename: Shih-Tien
authors.initials: ST
authors.lastname: Hsu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000653:Amniotic Fluid / Q000166:cytology*; D002454:Cell Differentiation / Q000528:radiation effects*; D002478:Cells, Cultured; D006801:Humans; D008027:Light; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D017382:Reactive Oxygen Species; D000469:Alkaline Phosphatase