title: Characterization of OXA-181, a carbapenem-hydrolyzing class D beta-lactamase from Klebsiella pneumoniae.
abstract: Klebsiella pneumoniae KP3 was isolated from a patient transferred from India to the Sultanate of Oman. K. pneumoniae KP3 was resistant to all β-lactams, including carbapenems, and expressed the carbapenem-hydrolyzing β-lactamase OXA-181, which differs from OXA-48 by four amino acid substitutions. Compared to OXA-48, OXA-181 possessed a very similar hydrolytic profile. The bla(OXA-181) gene was located on a 7.6-kb ColE-type plasmid and was linked to the insertion sequence ISEcp1. The ISEcp1-mediated one-ended transposition of bla(OXA-181) was also demonstrated.
authors.affiliation: Service de Bactériologie-Virologie-Hygiène, Hôpital de Bicêtre, 78 rue de Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Potron
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Emilie
authors.initials: E
authors.lastname: Lafeuille
authors.forename: Zaina
authors.initials: Z
authors.lastname: Al Maskari
authors.forename: Fatma
authors.initials: F
authors.lastname: Al Rashdi
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015780:Carbapenems / Q000378:metabolism* / Q000494:pharmacology*; D004251:DNA Transposable Elements; D005260:Female; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D004251:DNA Transposable Elements; D001618:beta-Lactamases; C063912:carbapenemase