title: Clinical and histological healing of a new collagen matrix in combination with the coronally advanced flap for the treatment of Miller class-I recession defects: an experimental study in the minipig.
abstract: OBJECTIVE
  To describe the histological and clinical outcomes of the use of a xenogeneic collagen matrix (CM) in combination with the coronally advanced flap (CAF) in the treatment of localized Miller class-I gingival recessions.
  
  
  METHODS
  Gingival recession defects were surgically created on 12 minipigs. The defects were randomly treated with either the CAF procedure and the interposition of a CM (test) or the CAF alone (control). Clinical and histological outcomes at 1, 4 and 12 weeks were evaluated.
  
  
  RESULTS
  Histometrically, in the test group, there was a shorter junctional epithelial dimension [2.26 (SD 0.23) mm] compared with the control [2.79 (SD 0.77) mm]. On the contrary, the amount of newly formed cementum was larger in the test group [1.08 (SD 0.41) mm] than in the control group [0.75 (SD 0.25) mm], although the differences were not statistically significant.
  
  
  CONCLUSIONS
  Both techniques rendered similar clinical outcomes, achieving complete root coverage at the end of the study. Nevertheless, the CM graft attained more tissue regeneration, characterized by a shorter epithelium and a larger new cementum formation. The use of a xenogeneic CM resulted in the incorporation of the xenograft within the adjacent host connective tissues in the absence of significant inflammation.
authors.affiliation: Graduate Periodontology Program, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.
authors.forename: Fabio
authors.initials: F
authors.lastname: Vignoletti
authors.forename: Javier
authors.initials: J
authors.lastname: Nuñez
authors.forename: Nicola
authors.initials: N
authors.lastname: Discepoli
authors.forename: Francesco
authors.initials: F
authors.lastname: De Sanctis
authors.forename: Raul
authors.initials: R
authors.lastname: Caffesse
authors.forename: Fernando
authors.initials: F
authors.lastname: Muñoz
authors.forename: Monica
authors.initials: M
authors.lastname: Lopez
authors.forename: Mariano
authors.initials: M
authors.lastname: Sanz
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000627:therapeutic use*; D003238:Connective Tissue / Q000637:transplantation*; D003739:Dental Cementum / Q000502:physiology*; D003746:Dental Enamel Proteins / Q000627:therapeutic use; D005260:Female; D005889:Gingival Recession / Q000601:surgery*; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D011897:Random Allocation; D012038:Regeneration; D013524:Surgical Flaps; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins