title: Efficacy of fipronil, amitraz and (S)-methoprene combination spot-on for dogs against adult dog fleas (Ctenocephalides canis, Curtis, 1826).
abstract: A novel spot-on formulation combining fipronil, amitraz and (S)-methoprene (CERTIFECT™, Merial Limited, GA, USA) was evaluated in adult Beagle dogs in a study to determine its adulticidal efficacy against the dog flea (Ctenocephalides canis, Curtis, 1826). Sixteen dogs were randomly allocated to treatment groups: 8 dogs served as untreated controls, and 8 dogs were treated once. Treatment consisted of applying a new combination formulation to deliver at least 6.7mg fipronil/kg body weight (bw), 8.0mg amitraz/kg bw, and 6.0mg (S)-methoprene/kg bw. The combination was designed to enhance the efficacy against ticks of the original fipronil/(S)-methoprene combination. Each dog was infested with 100 adult unfed dog fleas within 24h prior to treatment and then at weekly intervals for 8 weeks after treatment. At 24h after treatment or after each subsequent infestation, each dog was combed thoroughly to remove live fleas to be counted. A single treatment with CERTIFECT provided excellent knock-down of fleas within 24h after treatment and controlled re-infestations for up to 7 weeks (efficacy ≥96.5%, p<0.05).
authors.affiliation: Ecole Nationale Vétérinaire de Toulouse, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Bouhsira
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Yoon
authors.forename: Martine
authors.initials: M
authors.lastname: Roques
authors.forename: Coralie
authors.initials: C
authors.lastname: Manavella
authors.forename: Solange
authors.initials: S
authors.lastname: Vermot
authors.forename: Luiz G
authors.initials: LG
authors.lastname: Cramer
authors.forename: Catherine
authors.initials: C
authors.lastname: Ollagnier
authors.forename: Michel
authors.initials: M
authors.lastname: Franc
mesh_terms: D000287:Administration, Topical; D000818:Animals; D058251:Ctenocephalides / Q000187:drug effects* / Q000502:physiology; D004283:Dog Diseases / Q000188:drug therapy* / Q000469:parasitology / Q000517:prevention & control; D004285:Dogs; D004338:Drug Combinations; D005260:Female; D058267:Flea Infestations / Q000188:drug therapy / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D007306:Insecticides / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008726:Methoprene / Q000494:pharmacology / Q000627:therapeutic use; D011720:Pyrazoles / Q000494:pharmacology / Q000627:therapeutic use; D013983:Tick Control / Q000379:methods; D013997:Time Factors; D014052:Toluidines / Q000494:pharmacology / Q000627:therapeutic use; D016896:Treatment Outcome
chemical_list: D004338:Drug Combinations; D007306:Insecticides; D011720:Pyrazoles; D014052:Toluidines; C014983:amitraz; D008726:Methoprene; C082360:fipronil