title: Polymorphism of fecundity genes (FecB, FecX, and FecG) in the Indian Bonpala sheep.
abstract: The present study was designed for screening polymorphism of known fecundity genes in prolific Indian Bonpala sheep. Employing tetra-primer amplification refractory mutation system PCR, 11-point mutations of BMP1B, BMP15, and GDF9 genes of 97 Bonpala ewes were genotyped. The FecB locus of the BMPR1B gene and two loci (G1 and G4) of GDF9 gene were found to be polymorphic. In FecB locus, three genotypes, namely, wild type (Fec++, 0.02), heterozygous (FecB+, 0.23), and mutant (FecBB, 0.75) were detected. At G1 locus of GDF9 gene, three genotypes, namely, wild type (GG, 0.89), heterozygous (GA, 0.10), and mutant (AA, 0.01) were detected. At G4 locus of GDF9 gene, three genotypes, namely, wild type (AA, 0.01), heterozygous (AG, 0.14), and mutant (GG, 0.85) were detected. Statistically no significant correlation of polymorphism of FecB, G1, and G4 loci and litter size was found in this breed. All five loci of BMP15 and three loci of GDF 9 genes were monomorphic. This study reports Bonpala sheep as the first sheep breed where concurrent polymorphism at three important loci (FecB, G1, and G4) of two different fecundity genes (BMPR1B and GDF9) has been found.
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Joyabrata
authors.initials: J
authors.lastname: Roy
authors.forename: Shamik
authors.initials: S
authors.lastname: Polley
authors.forename: Sachinandan
authors.initials: S
authors.lastname: De
authors.forename: Ayan
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Subhasis
authors.initials: S
authors.lastname: Batabyal
authors.forename: Subhransu
authors.initials: S
authors.lastname: Pan
authors.forename: Biswajit
authors.initials: B
authors.lastname: Brahma
authors.forename: Tirtha Kumar
authors.initials: TK
authors.lastname: Datta
authors.forename: Surender Lal
authors.initials: SL
authors.lastname: Goswami
mesh_terms: D000818:Animals; D055430:Bone Morphogenetic Protein 15 / Q000235:genetics*; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics*; D016009:Chi-Square Distribution; D004587:Electrophoresis, Agar Gel; D005260:Female; D005298:Fertility; D056726:Genetic Association Studies; D055429:Growth Differentiation Factor 9 / Q000235:genetics*; D007194:India; D016014:Linear Models; D008098:Litter Size / Q000235:genetics*; D017354:Point Mutation; D020641:Polymorphism, Single Nucleotide; D012756:Sheep / Q000235:genetics*
chemical_list: D055430:Bone Morphogenetic Protein 15; D055429:Growth Differentiation Factor 9; D052005:Bone Morphogenetic Protein Receptors, Type I