title: Relationship of interleukin-8 gene polymorphisms with hepatocellular carcinoma susceptibility and pathological development.
abstract: OBJECTIVE
  Hepatocellular carcinoma (HCC) is one of the most frequent malignant neoplasms worldwide, and the second leading cause of death from cancer in Taiwan. Interleukin-8 (IL-8) is an angiogenic chemokine with important roles in the development and progression of many human malignancies including HCC. This study investigates the effects of single-nucleotide polymorphisms (SNPs) in the IL-8 gene on the susceptibility and clinicopathological characteristics of HCC.
  
  
  METHODS
  One hundred thirty-one HCC patients and 340 control subjects were analyzed for four IL-8 SNPs (-251 T/A, +781 C/T, +1633 C/T, and +2767 A/T) using PCR-RFLP genotyping analysis.
  
  
  RESULTS
  After adjusting for other confounders, results show that individuals with the IL-8 +781 T/T polymorphic genotype had a significantly lower risk of developing HCC than those with the wild-type (C/C) genotype (AOR = 0.346; 95% CI: 0.132-0.909). Multiple regression analysis showed that the presence of T/A or A/A at IL-8 -251 may indicate higher potential risk of hepatitis B infection (AOR = 2.847; 95% CI: 1.083-8.656). Additionally, these four IL-8 SNPs did not associate with liver-related clinicopathological markers in serum.
  
  
  CONCLUSIONS
  Genetic polymorphism at IL-8 +781 is an important factor in determining susceptibility to HCC in the Taiwanese population.
authors.affiliation: Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Chien
authors.forename: Chao-Bin
authors.initials: CB
authors.lastname: Yeh
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Li
authors.forename: Lin-Hung
authors.initials: LH
authors.lastname: Wei
authors.forename: Jer-Hwa
authors.initials: JH
authors.lastname: Chang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Peng
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Wu-Hsien
authors.initials: WH
authors.lastname: Kuo
mesh_terms: D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000453:epidemiology* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D008113:Liver Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D013624:Taiwan / Q000453:epidemiology
chemical_list: D016209:Interleukin-8