title: Butanol production from crystalline cellulose by cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4.
abstract: We investigated butanol production from crystalline cellulose by cocultured cellulolytic Clostridium thermocellum and the butanol-producing strain, Clostridium saccharoperbutylacetonicum (strain N1-4). Butanol was produced from Avicel cellulose after it was incubated with C. thermocellum for at least 24 h at 60°C before the addition of strain N1-4. Butanol produced by strain N1-4 on 4% Avicel cellulose peaked (7.9 g/liter) after 9 days of incubation at 30°C, and acetone was undetectable in this coculture system. Less butanol was produced by cocultured Clostridium acetobutylicum and Clostridium beijerinckii than by strain N1-4, indicating that strain N1-4 was the optimal strain for producing butanol from crystalline cellulose in this coculture system.
authors.affiliation: Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan. s3nakaya@nodai.ac.jp
authors.forename: Shunichi
authors.initials: S
authors.lastname: Nakayama
authors.forename: Keiji
authors.initials: K
authors.lastname: Kiyoshi
authors.forename: Toshimori
authors.initials: T
authors.lastname: Kadokura
authors.forename: Atsumi
authors.initials: A
authors.lastname: Nakazato
mesh_terms: D000440:Butanols / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
chemical_list: D000440:Butanols; D002482:Cellulose