title: [Performance of locked nucleic acid probe real-time polymerase chain reaction in the detection of Aspergillus fumigatus].
abstract: OBJECTIVE
  To evaluate the performance of locked nucleic acid (LNA) probe Real-time polymerase chain reaction (PCR) in the detection of Aspergillus fumigatus (A. fumigatus).
  
  
  METHODS
  All clinically cultured isolates of Aspergillus at our hospital were identified by morphology and DNA sequencing assay. The experimental group consists of A. fumigatus (n = 48) while the control group was made up of A. flavus (n = 55), A. versicolor (n = 16), A. nidulans (n = 10), A. sydowii (n = 5) and A. parasiticus (n = 1). The clinical samples consisted of A. fumigatus sinusitis tissue (n = 20) and bronchoalveolar lavage fluid (n = 1). DNA was extracted from all samples. A. fumigatus β-tubulin gene was targeted with LNA probe Real-time PCR assay. LNA probe Real-time PCR was evaluated with regards to specificity, efficiency, linear dynamic range in PCR amplification and limits of detection.
  
  
  RESULTS
  All clinical samples were positively amplified. The specificity was 100% and the PCR efficiency 98.2%. Linear dynamic range was at least six orders of magnitude and the limit of detection 2.5 pg.
  
  
  CONCLUSIONS
  LNA probe Real-time PCR is a promisingly accurate assay of rapidly detecting A. fumigatus practically and cost-efficiently.
authors.affiliation: Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Cao
authors.forename: Hai-Tong
authors.initials: HT
authors.lastname: Gu
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Lu
mesh_terms: D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015341:Nucleic Acid Probes; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D015341:Nucleic Acid Probes