title: The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition.
abstract: Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located at the sarcin/ricin loop of the larger rRNA. This cleavage inactivates ribosomes leading to protein biosynthesis inhibition and cell death. It has been proposed that interactions other than those found at the active site of ribotoxins are needed to explain their exquisite specific activity. The study presented shows the ability of a catalytically inactive α-sarcin mutant (H137Q) to bind eukaryotic ribosomes and interfere with in vitro protein biosynthesis. The results obtained are compatible with previous observations that α-sarcin can promote cell death by a mechanism that is independent of rRNA cleavage, expanding the potential set of activities performed by this family of toxins.
authors.affiliation: Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Alvarez-García
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Diago-Navarro
authors.forename: Elías
authors.initials: E
authors.lastname: Herrero-Galán
authors.forename: Lucía
authors.initials: L
authors.lastname: García-Ortega
authors.forename: Juan
authors.initials: J
authors.lastname: López-Villarejo
authors.forename: Nieves
authors.initials: N
authors.lastname: Olmo
authors.forename: Ramón
authors.initials: R
authors.lastname: Díaz-Orejas
authors.forename: José G
authors.initials: JG
authors.lastname: Gavilanes
authors.forename: Alvaro
authors.initials: A
authors.lastname: Martínez-del-Pozo
mesh_terms: D000818:Animals; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D008954:Models, Biological; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae
chemical_list: D005656:Fungal Proteins; D050505:Mutant Proteins; D011500:Protein Synthesis Inhibitors; C014801:alpha-sarcin; D004722:Endoribonucleases