title: Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction.
abstract: An interesting clinical option for optimizing healing tissue is the use of platelet concentrate. Platelets contain high quantities of growth factors, among these TGF-β1 and VEGF, which are known to be implicated in tissue regeneration. CGF is produced by processing blood samples with a special centrifuge device; three layers are formed: top acellular plasma (PPP), middle CGF and bottom red blood cells (RBC) layers. Given that to date there are no data concerning the biological characteristic of CGF, the aim of this study was to evaluate the presence of TGF-β1 and VEGF in CGF and also in PPP and RBC layers. In addition, since circulating stem cells are recruited from blood to injured tissue for healing we also evaluated the presence of CD34 positive cells. Our data show the presence of TGF-β1 and VEGF in CGF and RBC layers. In addition, we show CD34 positive cells in CGF.
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Division of Human Anatomy, University of Brescia, V.le Europa 11, 25123 Brescia, Italy. rodella@med.unibs.it
authors.forename: Luigi Fabrizio
authors.initials: LF
authors.lastname: Rodella
authors.forename: Gaia
authors.initials: G
authors.lastname: Favero
authors.forename: Ramon
authors.initials: R
authors.lastname: Boninsegna
authors.forename: Barbara
authors.initials: B
authors.lastname: Buffoli
authors.forename: Mauro
authors.initials: M
authors.lastname: Labanca
authors.forename: Giorgio
authors.initials: G
authors.lastname: Scarì
authors.forename: Luigi
authors.initials: L
authors.lastname: Sacco
authors.forename: Tiziano
authors.initials: T
authors.lastname: Batani
authors.forename: Rita
authors.initials: R
authors.lastname: Rezzani
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000032:analysis / Q000378:metabolism; D001773:Blood Cells / Q000737:chemistry* / Q000378:metabolism*; D002458:Cell Fractionation; D005260:Female; D005337:Fibrin / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D018952:Antigens, CD34; D036341:Intercellular Signaling Peptides and Proteins; D005337:Fibrin