title: Feasibility of a closed-system cell processor (ACP215) for automated preparation of washed platelet concentrates.
abstract: OBJECTIVE
  This study was aimed at evaluating the feasibility of the ACP215 closed-system cell processor for preparing washed platelet concentrates.
  
  
  METHODS
  Platelet washing was performed with either the ACP215 system or the manual technique with M-sol. Plasma protein removal and platelet recovery were estimated, and the washed platelet concentrates were stored for 5 days. Samples were collected after washing and on days 1, 3 and 5 of storage to determine the effects of the washing methods on the in vitro platelet qualities (platelet count, platelet volume, pH, glucose and lactate concentrations, hypotonic shock response, aggregation response and CD62P expression level).
  
  
  RESULTS
  Platelet recovery was 86·9 ± 2·1% and 85·9 ± 1·9% (P = 0·305), and plasma protein removal was 95·8 ± 0·9% and 96·9 ± 0·7% (P = 0·016) after washing with the ACP215 system and manual technique, respectively. No statistically significant differences in the in vitro platelet qualities were observed between the washing methods.
  
  
  CONCLUSIONS
  The ACP215 system is a feasible alternative to manual, labour-intensive, techniques for preparing washed platelet concentrates.
authors.affiliation: Japanese Red Cross Miyagi Blood Center, Sendai, Miyagi, Japan. s-oikawa@miyagi.bc.jrc.or.jp
authors.forename: S
authors.initials: S
authors.lastname: Oikawa
authors.forename: D
authors.initials: D
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Sawamura
authors.forename: T
authors.initials: T
authors.lastname: Itoh
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods*; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology; D001798:Blood Proteins / Q000302:isolation & purification; D005240:Feasibility Studies; D006801:Humans; D015539:Platelet Activation
chemical_list: D001798:Blood Proteins