title: [25-hydroxyvitamin D3-induced increases of normal human airway epithelial cell permeability is not mediated by upregulated ZO-1 expression].
abstract: OBJECTIVE
  To observe the effect of 25-hydroxyvitamin D3 on the permeability and ZO-1 expression in normal human airway epithelial cells.
  
  
  METHODS
  MTT assay was used to assess the viability of human airway epithelial cell line 16HBE following a 24-hour exposure to different concentrations of 25-hydroxy vitamin D3, and the transepithelial electrical resistance (TER) of the cell monolayer was measured using a Millicell-ERS voltohmmeter. Real-time quantitative RT-PCR was employed to determine the changes of ZO-1 mRNA expression in the cells following the exposures.
  
  
  RESULTS
  Exposure to 25-hydroxyvitamin D3 resulted in significantly increased permeability of 16HBE cells, but the exspression of ZO-1 showed no obvious changes. 25-hydroxyvitamin D3 at 4×10(-9) mol/L showed the strongest effect in increasing the permeability of cell monolayer.
  
  
  CONCLUSIONS
  25-hydroxyvitamin D3 increases the permeability of normal bronchial airway epithelial cell monolayer in vitro, but this effect is not mediated by upregulation of ZO-1 expression.
authors.affiliation: Department of Respiratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xiao-ting
authors.initials: XT
authors.lastname: Zhou
authors.forename: Hai-jin
authors.initials: HJ
authors.lastname: Zhao
authors.forename: Shao-xi
authors.initials: SX
authors.lastname: Cai
mesh_terms: D001980:Bronchi / Q000166:cytology* / Q000378:metabolism; D002112:Calcifediol / Q000493:pharmacokinetics / Q000494:pharmacology*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D002112:Calcifediol