title: A convenient method to measure blood-plasma concentration ratio using routine plasma collection in in vivo pharmacokinetic studies.
abstract: A practical time-saving method of determination of equilibrium blood-plasma concentration ratio is described. The method is based on the analysis of compound plasma concentrations in regular blood sample and the blood sample diluted with blank plasma. Since only plasma concentrations are analyzed, the method can be conveniently applied in routine pharmacokinetic studies with minimal additional work for obtaining blood-plasma ratio. The method can also be easily used in in vitro experiment. The results obtained by suggested method are in good agreement with that obtained by common in vitro measurements of blood-plasma ratio.
authors.affiliation: Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA. berezhkovskiy.leo@gene.com
authors.forename: Leonid M
authors.initials: LM
authors.lastname: Berezhkovskiy
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jonathan
authors.initials: J
authors.lastname: Cheong
mesh_terms: D000818:Animals; D001769:Blood / Q000378:metabolism*; D002850:Chromatography, Gel; D010599:Pharmacokinetics; D010949:Plasma / Q000378:metabolism*; D010953:Plasma Volume; D051381:Rats; D013048:Specimen Handling; D053719:Tandem Mass Spectrometry; D013816:Thermodynamics