title: Identification and characterization of the immunogenic cytotoxic TvCP39 proteinase gene of Trichomonas vaginalis.
abstract: TvCP39 is a 39 kDa cysteine proteinase (CP) involved in Trichomonas vaginalis cytotoxicity that has been found in vaginal secretions and is immunogenic in patients with trichomonosis. The goal of this work was to identify, clone, express, and characterize the tvcp39 gene. The tvcp39 gene was identified using a proteomic approach, and the complete gene was amplified using PCR, cloned, and sequenced. TvCP39 is encoded by a 915-bp cathepsin L-like CP gene. A fragment corresponding to the mature region (TvCP39r) was expressed, purified, and used to produce rabbit polyclonal antibodies and in functional assays. In one- and two-dimensional western blot assays, the anti-TvCP39r antibody reacted with two protein bands of ~28 and 27 kDa and three spots of ~28, 27, and 24 kDa in trichomonad proteinase-rich extracts that could correspond to the mature and processed fragments of the TvCP39 peptidase. The anti-TvCP39r antibody reacted with the parasitic surface and the native TvCP39 present in vaginal washes from patients with trichomonosis. Moreover, the recombinant TvCP39 protein bound to the surface of HeLa cells and protected HeLa cell monolayers from trichomonal destruction in a concentration-dependent manner. In conclusion, our data support TvCP39 as one of the surface proteinases that is glycosylated and is involved in trichomonal cytotoxicity. Thus, TvCP39 is the first glycosylated cysteine proteinase detected in T. vaginalis.
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN # 2508, Col. San Pedro Zacatenco, Delg. Gustavo A. Madero, CP 07360 Mexico, DF, Mexico. ramonluing@yahoo.com.mx
authors.forename: Lucero de Los Ángeles
authors.initials: Lde L
authors.lastname: Ramón-Luing
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Rendón-Gandarilla
authors.forename: Jonathan
authors.initials: J
authors.lastname: Puente-Rivera
authors.forename: Leticia
authors.initials: L
authors.lastname: Ávila-González
authors.forename: Rossana
authors.initials: R
authors.lastname: Arroyo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D056668:Cathepsin L / Q000378:metabolism; D003001:Cloning, Molecular; D057056:Cysteine Proteases / Q000737:chemistry* / Q000235:genetics* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005260:Female; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D014245:Trichomonas Infections / Q000382:microbiology*; D014246:Trichomonas vaginalis / Q000201:enzymology* / Q000235:genetics; D014621:Vagina / Q000382:microbiology
chemical_list: D015415:Biomarkers; D057056:Cysteine Proteases; D056668:Cathepsin L