title: ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance.
abstract: Multidrug and toxic compound extrusion (MATE) proteins comprise the most recently identified family of multidrug transporters. In plants, the numbers of MATE proteins has undergone a remarkable expansion, underscoring the importance of these transporters within this kingdom. Here, we describe the identification and characterization of Activated Disease Susceptibility 1 (ADS1) which encodes a putative MATE transport protein. An activation tagging screen uncovered the ads1-Dominant (ads1-D) mutant, which was subsequently characterized by molecular, genetic and biochemical approaches. The ads1-D mutant was compromised in both basal and nonhost resistance against microbial pathogens. Further, plant defence responses conferred by RPS4 were also disabled in ads1-D plants. By contrast, depletion of ADS1 transcripts by RNA-interference (RNAi) promoted basal disease resistance. Unexpectedly, ads1-D plants were found to constitutively accumulate reactive oxygen intermediates (ROIs). However, analysis of ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants indicated that an increase in ROIs did not impact ads1-D-mediated disease susceptibility. Our findings imply that ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression. Collectively, these data highlight an important role for MATE proteins in the establishment of plant disease resistance.
authors.affiliation: Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh, UK.
authors.forename: Xinli
authors.initials: X
authors.lastname: Sun
authors.forename: Eleanor M
authors.initials: EM
authors.lastname: Gilroy
authors.forename: Andrea
authors.initials: A
authors.lastname: Chini
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Nurmberg
authors.forename: Ingo
authors.initials: I
authors.lastname: Hein
authors.forename: Christophe
authors.initials: C
authors.lastname: Lacomme
authors.forename: Paul R J
authors.initials: PR
authors.lastname: Birch
authors.forename: Adil
authors.initials: A
authors.lastname: Hussain
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Yun
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Loake
mesh_terms: D020171:Botrytis / Q000378:metabolism; D060467:Disease Resistance; D004198:Disease Susceptibility; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D044224:Pseudomonas syringae / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism
chemical_list: D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D020156:Salicylic Acid