title: [Effect of bortezomib on the drug sensitivity of imatinib resistant K562/G01 cells].
abstract: OBJECTIVE
  To explore the effect of bortezomib (BOR) on the drug sensitivity of imatinib-resistant chronic myeloid leukemia cell line K562/G01 cell and its mechanism.
  
  
  METHODS
  MTT assay was used to detect the inhibition effect of cell growth, flow cytometry to cell cycle, and real time-PCR to the expression of COX-2 and mdr1 mRNA.
  
  
  RESULTS
  Combination of 10 and 20 nmol/L BOR with imatinib could significantly enhance the sensitivity of K562/G01 to imatinib, the reverse factor was 1.83 and 2.72-fold respectively. Cell cycle arrested at G(2)/M phase could be observed by flow cytometry on BOR treatment. The over-expression of COX-2 and mdr1 could be down-regulated by BOR.
  
  
  CONCLUSIONS
  BOR can enhance the imatinib sensitivity of imatinib resistant K562/G01 cell. The mechanism may be related to cell cycle phase arrested at G2/M and down-regulation of COX-2 and mdr1 expression.
authors.affiliation: Department of Hematology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Liang-Ming
authors.initials: LM
authors.lastname: Ma
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Li
authors.forename: Hua-Ping
authors.initials: HP
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Niu
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Ren
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001549:Benzamides; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D051546:Cyclooxygenase 2 / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D006801:Humans; D000068877:Imatinib Mesylate; D020014:K562 Cells; D010879:Piperazines / Q000494:pharmacology*; D011719:Pyrazines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D001549:Benzamides; D001897:Boronic Acids; D010879:Piperazines; D011719:Pyrazines; D011743:Pyrimidines; D000069286:Bortezomib; D000068877:Imatinib Mesylate; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human