title: Inflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot study.
abstract: Sympathetic ophthalmia is a bilateral uveitis that develops after penetrating injury to one eye. This study aimed to identify the inflammatory cellular sub-phenotypes and expression of pertinent inflammatory cytokines/chemokines in sympathetic ophthalmia (SO). Dalen-Fuchs nodules (DFN), granulomas, and non-granulomatous foci of inflammation were micro-dissected from 15 cases. RNA was extracted, and quantitative PCR was performed to measure IL-17, IL-18, IL-23, IFN-γ, CCL19, CXCL11, CCL17, and CCL22 transcripts. Immunohistochemical methods were used to characterize CD3, CD4, CD8, CD20, CD68, and CD163 expression. Non-granulomatous lymphocytes were predominantly CD3-positive and expressed more IFN-γ than cells within granulomas, consistent with Th1 cells. In contrast, granulomas and DFN contained mainly CD68+, CD163+/- and expressed more IL-17, IL-18, IL-23, CCL19, and CXCL11 than non-granulomatous cells. Our data indicate for the first time that M1 macrophages are the predominant inflammatory cells within granulomas and DFN of SO. We further observed high levels of IL-17 within granulomas and the presence of Th1 and M1 cells.
authors.affiliation: Immunopathology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Washington, DC, USA.
authors.forename: Emiko
authors.initials: E
authors.lastname: Furusato
authors.forename: DeFen
authors.initials: D
authors.lastname: Shen
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Cao
authors.forename: Bungo
authors.initials: B
authors.lastname: Furusato
authors.forename: Robert B
authors.initials: RB
authors.lastname: Nussenblatt
authors.forename: Elisabeth J
authors.initials: EJ
authors.lastname: Rushing
authors.forename: Chi-Chao
authors.initials: CC
authors.lastname: Chan
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018925:Chemokines / Q000096:biosynthesis*; D002648:Child; D016207:Cytokines / Q000096:biosynthesis*; D005131:Eye Injuries / Q000150:complications / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D042282:Microdissection; D008875:Middle Aged; D009879:Ophthalmia, Sympathetic / Q000378:metabolism* / Q000473:pathology; D010865:Pilot Projects; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D014605:Uveitis / Q000473:pathology; D055815:Young Adult
chemical_list: D018925:Chemokines; D016207:Cytokines; D012313:RNA