title: Reusable chromium-coated quartz crystal microbalance for immunosensing.
abstract: The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol.
authors.affiliation: Departamento de Ingeniería Eléctrica, Electrónica, Automática y Comunicaciones, ETSI Industriales, Universidad de Castilla La Mancha, 13071 Ciudad Real, Spain. MariaJesus.Oliver@uclm.es
authors.forename: M J
authors.initials: MJ
authors.lastname: Oliver
authors.forename: J
authors.initials: J
authors.lastname: Hernando-García
authors.forename: P
authors.initials: P
authors.lastname: Pobedinskas
authors.forename: K
authors.initials: K
authors.lastname: Haenen
authors.forename: A
authors.initials: A
authors.lastname: Ríos
authors.forename: J L
authors.initials: JL
authors.lastname: Sánchez-Rojas
mesh_terms: D000818:Animals; D002857:Chromium / Q000737:chemistry*; D006041:Goats; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000737:chemistry; D010084:Oxidation-Reduction; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D011817:Rabbits
chemical_list: D007074:Immunoglobulin G; D002857:Chromium