title: The fraction of myosin motors that participate in isometric contraction of rabbit muscle fibers at near-physiological temperature.
abstract: The duty ratio, or the part of the working cycle in which a myosin molecule is strongly attached to actin, determines motor processivity and is required to evaluate the force generated by each molecule. In muscle, it is equal to the fraction of myosin heads that are strongly, or stereospecifically, bound to the thin filaments. Estimates of this fraction during isometric contraction based on stiffness measurements or the intensities of the equatorial or meridional x-ray reflections vary significantly. Here, we determined this value using the intensity of the first actin layer line, A1, in the low-angle x-ray diffraction patterns of permeable fibers from rabbit skeletal muscle. We calibrated the A1 intensity by considering that the intensity in the relaxed and rigor states corresponds to 0% and 100% of myosin heads bound to actin, respectively. The fibers maximally activated with Ca(2+) at 4°C were heated to 31-34°C with a Joule temperature jump (T-jump). Rigor and relaxed-state measurements were obtained on the same fibers. The intensity of the inner part of A1 during isometric contraction compared with that in rigor corresponds to 41-43% stereospecifically bound myosin heads at near-physiological temperature, or an average force produced by a head of ~6.3 pN.
authors.affiliation: Institute of Mechanics, Lomonosov Moscow University, Moscow, Russia.
authors.forename: Andrey K
authors.initials: AK
authors.lastname: Tsaturyan
authors.forename: Sergey Y
authors.initials: SY
authors.lastname: Bershitsky
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Koubassova
authors.forename: Manuel
authors.initials: M
authors.lastname: Fernandez
authors.forename: Theyencheri
authors.initials: T
authors.lastname: Narayanan
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ferenczi
mesh_terms: D000818:Animals; D007537:Isometric Contraction / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000502:physiology*; D009218:Myosins / Q000378:metabolism*; D011817:Rabbits; D013696:Temperature; D014961:X-Ray Diffraction
chemical_list: D009218:Myosins