title: Proteome and cytokine serum profiling to diagnose a mycosis fungoides.
abstract: OBJECTIVE
  The aim of this study was to estimate a possibility of mycosis fungoides (MF) diagnostics based on protein profiling in blood serum.
  
  
  METHODS
  We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Protein profiling was carried out using SELDI TOF MS SELDI-TOF and also profiling of 27 cytokines with multiplex immunoassay technology was implemented.
  
  
  RESULTS
  MS data analysis of sera did not give satisfactory statistical discrimination between the groups. Antibody-based cytokine profiling revealed a number of cytokines with a change in their concentrations in both MF and psoriasis (IL-1Ra, IL-4, G-CSF). The C-X-C motif chemokine 10 (IP-10, CXCL10) cytokine had a significantly increased concentration (p<0,001) in samples from MF patients as compared with the other groups.
  
  
  CONCLUSIONS
  IP-10 may be considered as a promising biomarker for the differentiation between MF and other skin conditions.
authors.affiliation: Institute of Biomedical Chemistry, Moscow, Russia. sergei.moshkovskii@ibmc.msk.ru
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Moshkovskii
authors.forename: Elizaveta E
authors.initials: EE
authors.lastname: Sokolova
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Brattseva
authors.forename: Maria A
authors.initials: MA
authors.lastname: Karpova
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Pyatnitskiy
authors.forename: Anna A
authors.initials: AA
authors.lastname: Kubanova
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D054357:Chemokine CXCL10 / Q000097:blood*; D003937:Diagnosis, Differential; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D053590:Interleukin 1 Receptor Antagonist Protein / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009182:Mycosis Fungoides / Q000097:blood / Q000175:diagnosis*; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis*; D011565:Psoriasis / Q000097:blood / Q000175:diagnosis*; D012867:Skin; D012878:Skin Neoplasms / Q000097:blood / Q000175:diagnosis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D015415:Biomarkers; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D053590:Interleukin 1 Receptor Antagonist Protein; D020543:Proteome; D016179:Granulocyte Colony-Stimulating Factor; D015847:Interleukin-4