title: Ocular tropism of influenza A viruses: identification of H7 subtype-specific host responses in human respiratory and ocular cells.
abstract: Highly pathogenic avian influenza (HPAI) H7 virus infection in humans frequently results in conjunctivitis as a major symptom. However, our understanding of what properties govern virus subtype-specific tropism, and of the host responses responsible for eliciting ocular inflammation and pathogenicity following influenza virus infection, are not well understood. To study virus-host interactions in ocular tissue, we infected primary human corneal and conjunctival epithelial cells with H7, H5, and H1 subtype viruses. We found that numerous virus subtypes were capable of infecting and replicating in multiple human ocular cell types, with the highest titers observed with highly pathogenic H7N7 and H5N1 viruses. Similar patterns of proinflammatory cytokine and chemokine production following influenza virus infection were observed in ocular and respiratory cells. However, primary ocular cells infected with HPAI H7N7 viruses were found to have elevated levels of interleukin-1β (IL-1β), a cytokine previously implicated in ocular disease pathology. Furthermore, H7N7 virus infection of corneal epithelial cells resulted in enhanced and significant increases in the expression of genes related to NF-κB signal transduction compared with that after H5N1 or H1N1 virus infection. The differential induction of cytokines and signaling pathways in human ocular cells following H7 virus infection marks the first association of H7 subtype-specific host responses with ocular tropism and pathogenicity. In particular, heightened expression of genes related to NF-κB-mediated signaling transduction following HPAI H7N7 virus infection in primary corneal epithelial cells, but not respiratory cells, identifies activation of a signaling pathway that correlates with the ocular tropism of influenza viruses within this subtype.
authors.affiliation: Influenza Division, National Center for Immunization and Respiratory Diseases,Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Belser
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Katz
authors.forename: Terrence M
authors.initials: TM
authors.lastname: Tumpey
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004847:Epithelial Cells / Q000821:virology*; D005123:Eye / Q000821:virology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000276:immunology / Q000472:pathogenicity*; D011991:Receptors, Virus / Q000378:metabolism*; D012137:Respiratory System / Q000821:virology*; D015398:Signal Transduction; D056189:Viral Tropism
chemical_list: D016207:Cytokines; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011991:Receptors, Virus