title: Expression analysis of Clavata1-like and Nodulin21-like genes from Pinus sylvestris during ectomycorrhiza formation.
abstract: The ecology and physiology of ectomycorrhizal (EcM) symbiosis with conifer trees are well documented. In comparison, however, very little is known about the molecular regulation of these associations. In an earlier study, we identified three EcM-regulated Pinus expressed sequence tags (EST), two of which were identified as homologous to the Medicago truncatula nodulin MtN21. The third EST was a homologue to the receptor-like kinase Clavata1. We have characterized the expression patterns of these genes and of auxin- and mycorrhiza-regulated genes after induction with indole-3-butyric acid in Pinus sylvestris and in a time course experiment during ectomycorrhizal initiation with the co-inoculation of 2,3,5-triiodobenzoic acid, an auxin transport inhibitor. Our results suggest that different P. sylvestris nodulin homologues are associated with diverse processes in the root. The results also suggest a potential role of the Clv1-like gene in lateral root initiation by the ectomycorrhizal fungus.
authors.affiliation: Uppsala BioCenter, Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Box 7026, 75007 Uppsala, Sweden.
authors.forename: Gregory
authors.initials: G
authors.lastname: Heller
authors.forename: Karl
authors.initials: K
authors.lastname: Lundén
authors.forename: Roger D
authors.initials: RD
authors.lastname: Finlay
authors.forename: Frederick O
authors.initials: FO
authors.lastname: Asiegbu
authors.forename: Malin
authors.initials: M
authors.lastname: Elfstrand
mesh_terms: D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008565:Membrane Proteins / Q000096:biosynthesis*; D038821:Mycorrhizae / Q000254:growth & development* / Q000502:physiology; D041605:Pinus sylvestris / Q000235:genetics* / Q000382:microbiology* / Q000502:physiology; D010940:Plant Proteins / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D013559:Symbiosis
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins; C045625:nodulin; D020794:Receptor Protein-Tyrosine Kinases