title: Protective role of air potato (Dioscorea bulbifera) of yam family in myocardial ischemic reperfusion injury.
abstract: Hydroalcoholic extract of Dioscorea bulbifera (DB), a yam variety called air potato, was tested for its protective effect on myocardial ischemic/reperfusion (I/R) injury in rats due to apoptosis and necrosis. Myocardial I/R injury was induced by 30 min ischemia followed by 2 h reperfusion by perfusing isolated rat hearts with Krebs Henseilet bicarbonate (KHB) buffer in a Langendorff set up. Pretreatment of DB (150 mg kg(-1) body weight) for 30 days significantly reduced myocardial infarct size and improved the ventricular function (aortic flow and coronary flow, LVDP, LVmax dp/dt). Role of DB on apoptosis was also evaluated by determining caspase 3 as well as by examining pro-apoptotic and anti-apoptotic proteins Bax and Bcl2 by Western blot analysis followed by TUNEL assay. DB also prevented I/R-mediated down regulation of survival protein Akt and HO-1. Our results indicated that Dioscorea bulbifera could ameliorate myocardial ischemia and reperfusion injury by improving ventricular function and inhibition of cardiomyocyte necrosis and apoptosis.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, Tamilnadu, India.
authors.forename: Hannah Rachel
authors.initials: HR
authors.lastname: Vasanthi
authors.forename: Subhendu
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Diptarka
authors.initials: D
authors.lastname: Ray
authors.forename: Karuppiah Shanmugasundara
authors.initials: KS
authors.lastname: Pandian Jayachandran
authors.forename: Istvan
authors.initials: I
authors.lastname: Lekli
authors.forename: Dipak Kumar
authors.initials: DK
authors.lastname: Das
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002316:Cardiotonic Agents / Q000494:pharmacology*; D027742:Dioscorea / Q000737:chemistry*; D020287:In Situ Nick-End Labeling; D066298:In Vitro Techniques; D008297:Male; D009203:Myocardial Infarction / Q000188:drug therapy / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D009336:Necrosis; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D051017:Apoptosis Regulatory Proteins; D002316:Cardiotonic Agents; D010936:Plant Extracts