title: Selenium-containing allophycocyanin purified from selenium-enriched Spirulina platensis attenuates AAPH-induced oxidative stress in human erythrocytes through inhibition of ROS generation.
abstract: Both selenium and allophycocyanin (APC) have been reported to show novel antioxidant activities. In this study, a fast protein liquid chromatographic method for purification of selenium-containing allophycocyanin (Se-APC) from selenium-enriched Spirulina platensis and the protective effect of Se-APC on 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress have been described. After fractionation by ammonium sulfate precipitation, and separation by DEAE-Sepharose ion-exchange and Sephacryl S-300 size exclusion chromatography, Se-APC with purity ratio (A652/A280) of 5.30 and Se concentration of 343.02 μg g(-1) protein was obtained. Se-APC exhibited stronger antioxidant activity than APC by scavenging ABTS (2,2'-azinobis-3-ethylbenzothiazolin-6-sulfonic acid) and AAPH free radicals. The oxidative hemolysis and morphological changes induced by AAPH in human erythrocytes were effectively reversed by coincubation with Se-APC. Lipid oxidation induced by the pro-oxidant agent cupric chloride in human plasma, as evaluated by formation of conjugated diene, was blocked by Se-APC. The accumulation of malondialdehyde, loss of reduced glutathione, and increase in enzyme activities of glutathione peroxidase and reductase induced by AAPH in human erythrocytes were effectively suppressed by Se-APC. Furthermore, Se-APC significantly prevented AAPH-induced intracellular reactive oxygen species (ROS) generation. Taken together, our results suggest that Se-APC demonstrates application potential in treatment of diseases in which excess production of ROS acts as a casual or contributory factor.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou, Guangdong Province, China.
authors.forename: Haobin
authors.initials: H
authors.lastname: Zhang
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Yum-Shing
authors.initials: YS
authors.lastname: Wong
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010798:Phycocyanin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000097:blood*; D012643:Selenium / Q000008:administration & dosage / Q000032:analysis*; D053545:Spirulina / Q000737:chemistry*
chemical_list: D000578:Amidines; D000975:Antioxidants; D016877:Oxidants; D017382:Reactive Oxygen Species; C002497:allophycocyanin; D010798:Phycocyanin; C046728:2,2'-azobis(2-amidinopropane); D012643:Selenium