title: Evaluation of dentin formed in autogenous tooth transplantation in the dog: a comparison between one- and two-stage surgical techniques.
abstract: This study was designed to compare the thickness of dentin formed associated with autogenous tooth transplantation in dogs, using either one- or two-stage surgical techniques. The study consisted of three Beagles, older than 5 months, in which six incisors and six premolars were transplanted to mechanically prepared recipient sockets. One group was transplanted using a one-stage method to recipient beds prepared immediately before transplantation. The second groups of teeth were transplanted using a two-stage method in which the recipient beds were prepared and left to heal for 7 days before transplantation. Dogs were injected with xylenol orange, calcein and oxytetracycline at 2 days before, 3 and 9 weeks after transplantation, respectively, for vital staining. Clinical examinations were carried out every week, and the animals were euthanized 9 weeks later. The jaws were resected, fixed in formaldehyde and embedded in resin. Undemineralized sections were cut and examined by fluorescent microscopy. The thickness of dentin formed in the third week after transplantation and 9 weeks was evaluated by undertaking histomorphometric analysis and analysed using the Mann-Whitney U test (P = 0.05). All the transplanted teeth in both groups survived, and the dentin was formed. No statistically significant difference was found in the thickness of dentin formed in the third week and formed in the third to ninth week between the treatment groups (P = 0.999 and P = 0.998, respectively). This study demonstrated that there was no difference between the two surgical techniques in terms of the thickness of dentin formed in transplanted teeth.
authors.affiliation: Department of Dentistry, University School of Medicine, Coimbra, Portugal. m.mferreira@netcabo.pt
authors.forename: Manuel Marques
authors.initials: MM
authors.lastname: Ferreira
authors.forename: Maria Filomena Rabaça
authors.initials: MF
authors.lastname: Botelho
authors.forename: Lina
authors.initials: L
authors.lastname: Carvalho
authors.forename: Maria Reis
authors.initials: MR
authors.lastname: Silva
authors.forename: Barbara
authors.initials: B
authors.lastname: Oliveiros
authors.forename: Eunice Virgínia Palmeirão
authors.initials: EV
authors.lastname: Carrilho
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D003782:Dental Pulp / Q000098:blood supply / Q000502:physiology*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism*; D004285:Dogs; D008297:Male; D018919:Neovascularization, Physiologic; D014070:Tooth / Q000637:transplantation*; D020390:Tooth Socket / Q000601:surgery; D014182:Transplantation, Autologous / Q000379:methods*