title: Biocompatibility of PLGA/sP(EO-stat-PO)-coated mesh surfaces under constant shearing stress.
abstract: BACKGROUND
  In order to allow inflammatory response modification and ultimately improvement in tissue remodeling, we developed a new surface modification for meshes that will serve as a carrier for other substances. Biocompatibility is tested in an animal model.
  
  
  METHODS
  The animal model for diaphragmatic hernia repair was established in prior studies. Meshes were surface modified with star-configured PEO (polyethylene oxide)-based molecules [sP(EO-stat-PO)]. An electrospun nanoweb of short-term absorbable PLGA (polylactide-co-glycolide) with integrated sP(EO-stat-PO) molecules was applied onto the modified meshes. This coating also served as aerial sealing of the diaphragm. A final layer of hydrogel was applied to the product. Adhesive properties, defect size and mesh shrinkage were determined, and histological and immunohistochemical investigations performed after 4 months.
  
  
  RESULTS
  The mean defect size decreased markedly in both modified mesh groups. Histologically and with regard to apoptosis and proliferation rate, smooth muscle cells, collagen I/III ratio and macrophage count, no statistically significant difference was seen between the 3 mesh groups.
  
  
  CONCLUSIONS
  In this proof-of-principle investigation, we demonstrate good biocompatibility for this surface-modified mesh compared to a standard polypropylene-based mesh. This new coating represents a promising tool as a carrier for bioactive substances in the near future.
authors.affiliation: Department of Surgery, University Hospital, Technical University of Aachen, Aachen, Germany. gboehm@ukaachen.de
authors.forename: Gabriele
authors.initials: G
authors.lastname: Böhm
authors.forename: Yelena
authors.initials: Y
authors.lastname: Ushakova
authors.forename: Hamid Patrick
authors.initials: HP
authors.lastname: Alizai
authors.forename: Till
authors.initials: T
authors.lastname: Braunschweig
authors.forename: Christina
authors.initials: C
authors.lastname: Lente
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Heffels
authors.forename: Jürgen
authors.initials: J
authors.lastname: Groll
authors.forename: Ulf Peter
authors.initials: UP
authors.lastname: Neumann
authors.forename: Karsten
authors.initials: K
authors.lastname: Junge
mesh_terms: D000818:Animals; D017209:Apoptosis; D001672:Biocompatible Materials; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000009:adverse effects / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006548:Hernia, Diaphragmatic / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D059685:Herniorrhaphy / Q000009:adverse effects / Q000379:methods; D008264:Macrophages / Q000473:pathology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000009:adverse effects / Q000737:chemistry*; D011098:Polyglactin 910 / Q000009:adverse effects / Q000737:chemistry*; D011817:Rabbits; D033081:Shear Strength; D013314:Stress, Mechanical; D013526:Surgical Mesh / Q000009:adverse effects; D000267:Tissue Adhesions / Q000209:etiology / Q000473:pathology; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D003094:Collagen