title: Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris.
abstract: Trichoderma reesei cellulases are important biocatalysts for a wide range of industrial applications that include the paper, feed, and textile industries. T. reesei endoglucanase 1 (egl1) was successfully expressed as an active and stable catalyst in Pichia pastoris for the first time. Codon optimization was applied to egl1 of T. reesei to enhance its expression levels in P. pastoris. When compared with the originally cloned egl1 gene of T. reesei, the synthetic codon optimized egl1 gene (egl1s) was expressed at a higher level in P. pastoris. Batch fermentations of both clones with the same copy number under controlled conditions indicated that codon optimized EGI enzyme activity increased to 1.24 fold after 72 h of methanol induction. Our research indicated that P. pastoris is a suitable host for cellulase production.
authors.affiliation: Sabanci University, Biological Sciences and Bioengineering, Istanbul, Turkey. gunseli@sabanciuniv.edu
authors.forename: Gunseli Bayram
authors.initials: GB
authors.lastname: Akcapinar
authors.forename: Ozgur
authors.initials: O
authors.lastname: Gul
authors.forename: Ugur
authors.initials: U
authors.lastname: Sezerman
mesh_terms: D001483:Base Sequence; D019149:Bioreactors; D002384:Catalysis; D002480:Cellulase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003062:Codon; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005800:Genes, Fungal; D010843:Pichia / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D003062:Codon; D017931:DNA Primers; D011994:Recombinant Proteins; C056374:endoglucanase 1; D002480:Cellulase