title: Developmental toxicity testing for safety assessment: new approaches and technologies.
abstract: The ILSI Health and Environmental Sciences Institute's Developmental and Reproductive Toxicology Technical Committee held a 2-day workshop entitled "Developmental Toxicology-New Directions" in April 2009. The fourth session of this workshop focused on new approaches and technologies for the assessment of developmental toxicology. This session provided an overview of the application of genomics technologies for developmental safety assessment, the use of mouse embryonic stem cells to capture data on developmental toxicity pathways, dynamical cell imaging of zebrafish embryos, the use of computation models of development pathways and systems, and finally, high-throughput in vitro approaches being utilized by the EPA ToxCast program. Issues discussed include the challenges of anchoring in vitro predictions to relevant in vivo endpoints and the need to validate pathway-based predictions with targeted studies in whole animals. Currently, there are 10,000 to 30,000 chemicals in world-wide commerce in need of hazard data for assessing potential health risks. The traditional animal study designs for assessing developmental toxicity cannot accommodate the evaluation of this large number of chemicals, requiring that alternative technologies be utilized. Though a daunting task, technologies are being developed and utilized to make that goal reachable.
authors.affiliation: National Center for Computational Toxicology, US Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. knudsen.thomas@epa.gov
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Knudsen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kavlock
authors.forename: George P
authors.initials: GP
authors.lastname: Daston
authors.forename: Donald
authors.initials: D
authors.lastname: Stedman
authors.forename: Mary
authors.initials: M
authors.lastname: Hixon
authors.forename: James H
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects*; D053595:Embryonic Stem Cells / Q000187:drug effects; D047109:Fetal Development / Q000187:drug effects*; D006801:Humans; D051379:Mice; D018570:Risk Assessment; D012449:Safety; D015398:Signal Transduction; D013672:Technology; D018675:Toxicity Tests / Q000379:methods*; D015027:Zebrafish / Q000196:embryology