title: Effects of PerClot® on the healing of full-thickness skin wounds in rats.
abstract: PerClot(®) is a hemostatic material made of polysaccharide from modified starch and has been shown to assist in topical hemostasis. The principal goal in treating surgical and non-surgical wounds is the need for rapid closure of the lesion. This study investigated whether topical application of PerClot(®) could improve impaired wound healing in Sprague-Dawley (SD) rats. Full-thickness skin wounds were created on the back of the rats. Immediately, PerClot(®) was introduced into the wound bed, while wounds receiving starch or nothing served as controls. Wound closure was monitored using well-recognized wound-healing parameters: histological examination for inflammatory cells and fibroblast infiltration, newly formed capillaries, and collagen deposition. Meanwhile, transforming growth factor (TGF-β1) was measured by immunochemistry. Wound closure was significantly accelerated by local application of PerClot(®). Furthermore, PerClot(®)-treated wounds showed significantly increased fibroblast numbers at 5 days post-wounding, and newly formed capillaries at 7 days post-wounding, and collagen regeneration at 7 and 14 days post-wounding. The number of infiltrating fibroblasts expressing TGF-β1 was significantly higher than that in the controls at 7 and 14 days post-wounding. PerClot(®) can improve the wound healing and this effect might involve an increase in the activity of fibroblasts and increased release of TGF-β1.
authors.affiliation: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xi'an, China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Haiyin
authors.initials: H
authors.lastname: Dong
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Pengtao
authors.initials: P
authors.lastname: Zhao
authors.forename: Wen
authors.initials: W
authors.lastname: Niu
authors.forename: Dunquan
authors.initials: D
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Ji
authors.forename: Chen
authors.initials: C
authors.lastname: Xing
authors.forename: Dongding
authors.initials: D
authors.lastname: Lu
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Li
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002196:Capillaries / Q000502:physiology; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006487:Hemostasis / Q000187:drug effects / Q000502:physiology; D006490:Hemostatics / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply / Q000187:drug effects / Q000293:injuries; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology
chemical_list: D006490:Hemostatics; D053773:Transforming Growth Factor beta1; D003094:Collagen