title: Scaffoldin modules serving as "cargo" domains to promote the secretion of heterologous cellulosomal cellulases by Clostridium acetobutylicum.
abstract: The secretion of large heterologous cellulases by Clostridium acetobutylicum was formerly shown to be deleterious. To circumvent this issue, various scaffoldins' modules were grafted at their N termini. Family 3a cellulose binding module combined with an X2 module(s) was found to trigger the secretion of Clostridium cellulolyticum cellulases by the solventogenic bacterium.
authors.affiliation: Laboratoire de Chimie Bactérienne CNRS IMM, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Angélique
authors.initials: A
authors.lastname: Chanal
authors.forename: Florence
authors.initials: F
authors.lastname: Mingardon
authors.forename: Marielle
authors.initials: M
authors.lastname: Bauzan
authors.forename: Chantal
authors.initials: C
authors.lastname: Tardif
authors.forename: Henri-Pierre
authors.initials: HP
authors.lastname: Fierobe
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D044602:Cellulases / Q000235:genetics / Q000378:metabolism*; D044603:Cellulosomes / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000201:enzymology* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins; C074074:cellulose binding protein A, Clostridium cellulovorans; D044602:Cellulases