title: Lack of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is accompanied by increased CK2α' levels.
abstract: DNA-PKcs is the catalytic subunit of DNA-dependent protein kinase, an enzyme necessary for non-homologous end-joining (NHEJ) and hence repair of DNA double strand breaks. Characterization of two isogenic cell lines, M059K and M059J, which are DNA-PKcs-proficient and -deficient, respectively, revealed that lack of DNA-PKcs is accompanied by an increase in the protein level of one of the catalytic isozymes of protein kinase CK2, i.e., CK2α' and a concomitant increase in CK2 activity. The increase was also detectable at the mRNA level as measured by quantitative real time PCR. However, no increase at the DNA level was observed either by comparative PCR or fluorescent in situ hybridization indicating that gene amplification is not involved. Interestingly, only CK2α' was increased and not the other two subunits of CK2, i.e., CK2β or CK2α. In addition, the increase in CK2α' protein level was also observed in a DNA-PKcs-deficient mouse cell line.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense, Denmark.
authors.forename: Birgitte B
authors.initials: BB
authors.lastname: Olsen
authors.forename: Ulrike
authors.initials: U
authors.lastname: Fischer
authors.forename: Tine L
authors.initials: TL
authors.lastname: Rasmussen
authors.forename: Matthias
authors.initials: M
authors.lastname: Montenarh
authors.forename: Eckart
authors.initials: E
authors.lastname: Meese
authors.forename: Gerhard
authors.initials: G
authors.lastname: Fritz
authors.forename: Olaf-Georg
authors.initials: OG
authors.lastname: Issinger
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D045744:Cell Line, Tumor; D051747:DNA-Activated Protein Kinase / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology; D005784:Gene Amplification; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000201:enzymology / Q000235:genetics; D006801:Humans; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D047390:Casein Kinase II; D051747:DNA-Activated Protein Kinase