title: Nuclear receptor profiling of ovarian granulosa cell tumors.
abstract: Granulosa cell tumors of the ovary (GCT) represent ~5% of malignant ovarian tumors. The adult form is defined by a mutation in the FOXL2 gene. GCT exhibit many of the features of normal proliferating granulosa cells. We have profiled the expression of the 48 human nuclear receptors (NR) by quantitative RT-PCR in a panel of GCT and in two GCT-derived cell lines, COV434 and KGN. The highest level of expression is seen for COUP-TF2 with abundant expression of PPARγ, SF-1, and TR-α. Estrogen receptor (ER)-β is the most abundant of the steroid receptors with relatively high expression also of AR, ER-α, and PR. The concordance of expression for each NR across the tumors is remarkably high with same discordance between the cell lines and the tumors, particularly the COV434 line. No significant differences were observed with respect to tumor stage for NR expression. These findings provide a full profile of NR expression in GCT which will enable full characterization of their roles and potential as therapeutic targets.
authors.affiliation: Prince Henry's Institute of Medical Research, Institute of Medical Research, Clayton, Victoria 3168, Australia.
authors.forename: Maria
authors.initials: M
authors.lastname: Alexiadis
authors.forename: Natalie
authors.initials: N
authors.lastname: Eriksson
authors.forename: Stacey
authors.initials: S
authors.lastname: Jamieson
authors.forename: Melissa
authors.initials: M
authors.lastname: Davis
authors.forename: Ann E
authors.initials: AE
authors.lastname: Drummond
authors.forename: Simon
authors.initials: S
authors.lastname: Chu
authors.forename: Colin D
authors.initials: CD
authors.lastname: Clyne
authors.forename: George E
authors.initials: GE
authors.lastname: Muscat
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Fuller
mesh_terms: D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000235:genetics; D020869:Gene Expression Profiling; D006106:Granulosa Cell Tumor / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C421604:FOXL2 protein, human; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D018160:Receptors, Cytoplasmic and Nuclear