title: Nisin quantification by ELISA allows the modeling of its apparent diffusion coefficient in model cheeses.
abstract: The diffusion of small solutes in cheese is of key importance for most enzymatic reactions involved in the ripening process. However, only a limited amount of data is available on salt diffusion and practically none on peptide diffusion. Nisin, a bacteriocin peptide, migrated in model cheeses made from ultrafiltered (UF) retentate. A profile concentration device and an enzyme-linked immunosorbent assay (ELISA), specifically developed for nisin quantification in cheese, were used to model the apparent diffusion coefficients for nisin according to Fick's law. This average coefficient was 49.5 μm(2)/s in UF cheese (n = 2). When 10% gelatin was added to the retentate, this value decreased to 34.4 μm(2)/s (n = 2). The two cheeses differed in their macrostructure (rheology) and microstructure (confocal microscopy). This study provides the first apparent diffusion coefficients for a peptide in cheese and supports the hypothesis that composition and structure influence the diffusion of small solutes such as peptides.
authors.affiliation: INRA, UMR1253, Science and Technology of Milk and Eggs, F-35000 Rennes, France.
authors.forename: Samar
authors.initials: S
authors.lastname: Aly
authors.forename: Juliane
authors.initials: J
authors.lastname: Floury
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Famelart
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Madec
authors.forename: Didier
authors.initials: D
authors.lastname: Dupont
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Gouar
authors.forename: Sylvie
authors.initials: S
authors.lastname: Lortal
authors.forename: Sophie
authors.initials: S
authors.lastname: Jeanson
mesh_terms: D002611:Cheese / Q000032:analysis*; D055598:Chemical Phenomena; D004058:Diffusion; D004797:Enzyme-Linked Immunosorbent Assay; D005511:Food Handling / Q000379:methods; D018613:Microscopy, Confocal; D009561:Nisin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D009561:Nisin