title: [Antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin against herpes simplex virus 2 in vitro].
abstract: OBJECTIVE
  To investigate the antiviral activity of 3-hydroxyphthalic anhydride-modified ovalbumin (HP-OVA) against herpes simplex virus 2 (HSV-2) in vitro.
  
  
  METHODS
  By chemical modification, ovalbumin (OVA) was treated with 3-hydroxyphthalic anhydride (HP) to prepare HP-OVA. The anti-HSV-2 activity against HSV-2 333 virus in vitro and the cytotoxicity of HP-OVA in African green monkey kidney cells (Vero cells) were detected by MTT colorimetric assay. The inhibitory effects of HP-OVA on 17 strains of vaginal lactobacilli were observed by microscopy.
  
  
  RESULTS
  Anhydride-modified ovalbumin significantly inhibited the infection by HSV-2 with an IC(50) of 23.56±8.33 µg/ml. HP-OVA showed only low cytotoxicity to the host cells with a CC(50) over 1 mg/ml. HP-OVA did not produce significant inhibitory effect on the 17 strains of vaginal lactobacilli (MIC>1 mg/ml).
  
  
  CONCLUSIONS
  Anhydride-modified protein HP-OVA exhibits potent anti-HSV-2 activity in vitro and can be a good microbicide candidate for prevention of sexually transmitted diseases.
authors.affiliation: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. helili-1@yahoo.com
authors.forename: Li-li
authors.initials: LL
authors.lastname: He
authors.forename: Jiang-man
authors.initials: JM
authors.lastname: Duan
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Qiu
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.forename: Shu-wen
authors.initials: SW
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D010047:Ovalbumin / Q000737:chemistry / Q000494:pharmacology*; D010796:Phthalic Anhydrides / Q000737:chemistry* / Q000494:pharmacology; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D010796:Phthalic Anhydrides; C478070:3-hydroxyphthalic anhydride; D010047:Ovalbumin