title: Protective effects of hydrogen-rich saline in preeclampsia rat model.
abstract: Hydrogen has been reported as a novel antioxidant to selectively reduce levels of toxic reactive-oxygen species (ROS). We investigated the effects of hydrogen-rich saline on the prevention of oxidative injuries in N(omega)-nitro-L-arginine methyl ester (L-NAME) induced rat model of preeclampsia (PE). Sprague-Dawley rats (n = 50) were randomized into five groups: non-pregnant; normal pregnancy; pregnancy + hydrogen saline, 5 ml/kg, intraperitoneal (i.p.); pregnancy + L-NAME, 60 mg/kg (i.p.); pregnancy + L-NAME + hydrogen saline rats. Terminations of pregnancy were performed on day 22 of gestation, when the placentas and kidneys were microscopically inspected; tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and malonyldialdehyde (MDA) were assessed; and the mean systolic BP, level of proteinuria, resorptions, and pups birth weights were recorded. It was found that the pups of hypertensive gravid rats treated with hydrogen-rich saline presented fewer number of resorptions than those of the group of pregnancy + L-NAME, 60 mg/kg i.p. (P < 0.05). Additionally, hydrogen-rich saline treatment decreased the blood and placental MDA, proteinuria and the pro-inflammatory cytokine TNF-α, IL-1β in the placental tissues compared with those in L-NAME-treated rats (all P < 0.05). The mean systolic BP showed no significant difference except on day 22 of gestation (P < 0.05). The preventive administration of hydrogen significantly attenuated the severity of PE, which might be ascribed to a reduction in inflammation response and oxidative stress. It could be concluded that hydrogen can be an effective antioxidant in the management of PE.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, 389 Xincun Rd., Shanghai 200065, PR China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, PR China.
authors.forename: X
authors.initials: X
authors.lastname: Sun
authors.affiliation: International Peace Maternity and Child Health Hospital, Jiaotong University, 200030 Shanghai, PR China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital of Tongji University, 389 Xincun Rd., Shanghai 200065, PR China. Electronic address: Xiaowen_tong@yahoo.com.
authors.forename: X
authors.initials: X
authors.lastname: Tong
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D005327:Fetal Resorption; D006859:Hydrogen / Q000627:therapeutic use*; D053583:Interleukin-1beta / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester; D011225:Pre-Eclampsia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D011247:Pregnancy; D011256:Pregnancy Outcome; D011507:Proteinuria / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012462:Saline Solution, Hypertonic / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000975:Antioxidants; D053583:Interleukin-1beta; D012462:Saline Solution, Hypertonic; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D006859:Hydrogen; D019331:NG-Nitroarginine Methyl Ester