title: Visualization of MG53-mediated cell membrane repair using in vivo and in vitro systems.
abstract: Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular understanding of the basic biology of tissue repair, as well as for potential translational applications in regenerative medicine. Here we detail the experimental protocols for exploring the in vivo function of MG53 in repair of muscle injury using treadmill exercise protocols on mouse models, for testing the ex vivo membrane repair capacity by measuring dye entry into isolated muscle fibers, and for monitoring the dynamic process of MG53-mediated vesicle trafficking and cell membrane repair in cultured cells using live cell confocal microscopy.
authors.affiliation: Department of Physiology and Biophysics, Robert Wood Johnson Medical School, USA. noah.weisleder@umdnj.edu
authors.forename: Noah
authors.initials: N
authors.lastname: Weisleder
authors.forename: Peihui
authors.initials: P
authors.lastname: Lin
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.forename: Matthew
authors.initials: M
authors.lastname: Orange
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Jianjie
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis* / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry* / Q000502:physiology*; D005080:Exercise Test / Q000379:methods; D005528:Foot / Q000502:physiology; D006614:Hindlimb / Q000502:physiology; D008565:Membrane Proteins; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000293:injuries / Q000502:physiology
chemical_list: D002352:Carrier Proteins; C538824:MG53 protein, mouse; D008565:Membrane Proteins