title: Solution structure of a short-chain insecticidal toxin LaIT1 from the venom of scorpion Liocheles australasiae.
abstract: The solution structure of an insecticidal toxin LaIT1, a 36-residue peptide with a unique amino-acid sequence and two disulfide bonds, isolated from the venom of the scorpion Liocheles australasiae was determined by heteronuclear NMR spectroscopy. Structural similarity search showed that LaIT1 exhibits an inhibitory cystine knot (ICK)-like fold, which usually contains three or more disulfide bonds. Mutational analysis has revealed that two Arg residues of LaIT1, Arg(13) and Arg(15), play significant roles in insecticidal activity.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Japan.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Horita
authors.forename: Nobuto
authors.initials: N
authors.lastname: Matsushita
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kawachi
authors.forename: Reed
authors.initials: R
authors.lastname: Ayabe
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nagata
authors.forename: Hisashi
authors.initials: H
authors.lastname: Miyagawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004252:DNA Mutational Analysis; D007306:Insecticides / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics; D012605:Scorpions / Q000378:metabolism*
chemical_list: D007306:Insecticides; D010455:Peptides; D012604:Scorpion Venoms