title: [Variability of urine proteome of healthy persons during 105-daily isolation].
abstract: Human proteome is very plastic, it changes under the influence of various biological factors. It is of big interest to find out how specific factors of an environment, including a long-term isolation affect on urine proteome. The study was conducted during the experiment with 105-day isolation. In the present investigation we collected urine samples from 6 healthy volunteers (26-41 years old). The physical activity, daily rhythms and diet were controlled. Urine samples were fractionated on magnetic beads MB-HIC C8 (MB - hydrophobic-interaction chromatography) with ClinProt robot (Bruker Daltonics) prior to MALDI-TOF mass spectrometer analysis with Autoflex III TOF/TOF (Bruker Daltonics), working in a positive linear mode. 117 peaks have been obtained in each spectrum of urine. We have shown that even during isolation and under controlled conditions of life a high variability of urine proteome of healthy personas (36 protein MC-peaks in the urine, on average) are revealed.
authors.forename: O A
authors.initials: OA
authors.lastname: Valeeva
authors.forename: L Kh
authors.initials: LKh
authors.lastname: Pastushkov
authors.forename: N A
authors.initials: NA
authors.lastname: Pakharukova
authors.forename: I V
authors.initials: IV
authors.lastname: Dobrokhotov
authors.forename: I M
authors.initials: IM
authors.lastname: Larina
mesh_terms: D000328:Adult; D018504:Ecological Systems, Closed; D006801:Humans; D008297:Male; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013997:Time Factors; D014556:Urine
chemical_list: D020543:Proteome