title: Advances in DNA-based techniques for the detection of seafood species substitution on the commercial market.
abstract: Increased worldwide trade and processing of seafood has increased the potential for species substitution on the commercial market. To detect and prevent species substitution, several deoxyribonucleic acid (DNA)-based methods have been developed that can be used to identify species in a variety of food types. For large-scale applications, such as regulatory screening, these methods must be rapid, cost-effective, reliable, and have high potential for automation. This review highlights recent technological advances in DNA-based identification methods, with a focus on seafood species identification in automated, high-throughput settings. Advances in DNA isolation methods include silica-based columns for use in high-throughput operations and magnetic bead particles for increased and targeted recovery of DNA. The three most widely used methods for seafood species identification (polymerase chain reaction [PCR] sequencing, PCR-restriction fragment length polymorphism, and species-specific PCR) will be discussed, with a focus on the incorporation of technologies such as rapid PCR cycling, microfluidic chips, and real-time PCR. Emerging methods, including DNA microarrays and next-generation sequencing will also be explored for their potential to identify seafood species on a large scale. Overall, many of the technological advances discussed here offer complementary properties that will enable species identification in a variety of settings and with a range of products.
authors.affiliation: Food Science and Technology, Food Innovation Center, Oregon State University, Portland, OR 97209, USA. Rosalee.hellberg@gmail.com
authors.forename: Rosalee S Rasmussen
authors.initials: RS
authors.lastname: Hellberg
authors.forename: Michael T
authors.initials: MT
authors.lastname: Morrissey
mesh_terms: D000818:Animals; D057205:Automation, Laboratory; D049872:Bivalvia / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000737:chemistry; D016172:DNA Fingerprinting / Q000379:methods*; D005399:Fishes / Q000235:genetics; D057166:High-Throughput Screening Assays; D046210:Microfluidic Analytical Techniques; D058889:Molecular Typing / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D017747:Seafood / Q000032:analysis / Q000145:classification* / Q000592:standards; D013045:Species Specificity
chemical_list: D004247:DNA