title: Mitochondrial targeting of peroxiredoxin 5 is preserved from annelids to mammals but is absent in pig Sus scrofa domesticus.
abstract: Peroxiredoxin 5 (PRDX5) is a thioredoxin peroxidase able to reduce hydrogen peroxide, alkyl hydroperoxides and peroxynitrite. In human, PRDX5 was reported to be localized in the cytosol, the mitochondria, the peroxisomes and the nucleus. Mitochondrial localization results from the presence of an N-terminal mitochondrial targeting sequence (MTS). Here, we examined the conservation of mitochondrial localization of PRDX5 in animal species. We found that PRDX5 MTS is present and functional in the annelid lugworm Arenicola marina. Surprisingly, although mitochondrial targeting is well conserved among animals, PRDX5 is missing in mitochondria of domestic pig. Thus, it appears that mitochondrial targeting of PRDX5 may have been lost throughout evolution in animal species, including pig, with unknown functional consequences.
authors.affiliation: Université catholique de Louvain, Institut des Sciences de la Vie, 4-5 place Croix du Sud, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Valérie
authors.initials: V
authors.lastname: Van der Eecken
authors.forename: André
authors.initials: A
authors.lastname: Clippe
authors.forename: Paul P
authors.initials: PP
authors.lastname: Van Veldhoven
authors.forename: Bernard
authors.initials: B
authors.lastname: Knoops
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D006801:Humans; D008322:Mammals; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000378:metabolism*; D011077:Polychaeta; D021382:Protein Sorting Signals; D021381:Protein Transport; D016415:Sequence Alignment
chemical_list: D021382:Protein Sorting Signals; D054464:Peroxiredoxins