title: [Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2].
abstract: The inhibitory ratio (1%) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and 1% was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC50) was about 2.26 mg x mL(-1). There was a good relationship between the logarithm concentrations of artificial musk and 1% when the concentrations of artificial musk ranged from 0.31-20.0 mg x mL(-1). The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.
authors.affiliation: China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Luo
authors.forename: Cheng
authors.initials: C
authors.lastname: Jin
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Rui-qing
authors.initials: RQ
authors.lastname: Wen
authors.forename: Xing-feng
authors.initials: XF
authors.lastname: Li
authors.forename: Rui-sheng
authors.initials: RS
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D007124:Immunoenzyme Techniques / Q000379:methods; D020128:Inhibitory Concentration 50; D021281:Quantitative Structure-Activity Relationship
chemical_list: D000893:Anti-Inflammatory Agents; D052246:Cyclooxygenase 2 Inhibitors; D005229:Fatty Acids, Monounsaturated; C008563:musk; D051546:Cyclooxygenase 2