title: Human papillomavirus genotype distribution in Czech women and men with diseases etiologically linked to HPV.
abstract: BACKGROUND
  The HPV prevalence and genotype distribution are important for the estimation of the impact of HPV-based cervical cancer screening and HPV vaccination on the incidence of diseases etiologically linked to HPVs. The HPV genotype distribution varies across different geographical regions. Therefore, we investigated the type-specific HPV prevalence in Czech women and men with anogenital diseases.
  
  
  METHODS
  We analyzed 157 squamous cell carcinoma samples, 695 precancerous lesion samples and 64 cervical, vulvar and anal condylomata acuminate samples. HPV detection and typing were performed by PCR with GP5+/6+ primers, reverse line blot assay and sequencing.
  
  
  RESULTS
  Thirty different HPV genotypes were detected in our study, HPV 16 being the most prevalent type both in precancerous lesions (45%) and squamous cell carcinomas (59%). In benign lesions, HPV 6 (72%) was the most common type. Altogether, 61% of carcinoma samples and 43% of precancerous lesion samples contained HPV 16 and/or 18. The presence of HPV types related to the vaccinal ones (HPV 31, 45, 33, 52, 58) were detected in 16% of carcinoma samples and 18% of precancerous lesion samples. HPV 16 and/or 18 were present in 76% of cervical cancer samples, 33% of CIN1, 43% CIN2 and 71% of CIN3 samples. HPV types 6 and/or 11 were detected in 84% samples of condylomata acuminate samples.
  
  
  CONCLUSIONS
  The prevalence of vaccinal and related HPV types in patients with HPV-associated diseases in the Czech Republic is very high. We may assume that the implementation of routine vaccination against HPV would greatly reduce the burden of HPV-associated diseases in the Czech Republic.
authors.affiliation: Department of Experimental Virology, Institute of Hematology and Blood Transfusion, Prague, Czech Republic. rutach@uhkt.cz
authors.forename: Ruth
authors.initials: R
authors.lastname: Tachezy
authors.forename: Jana
authors.initials: J
authors.lastname: Smahelova
authors.forename: Martina
authors.initials: M
authors.lastname: Salakova
authors.forename: Marc
authors.initials: M
authors.lastname: Arbyn
authors.forename: Lukas
authors.initials: L
authors.lastname: Rob
authors.forename: Petr
authors.initials: P
authors.lastname: Skapa
authors.forename: Tomas
authors.initials: T
authors.lastname: Jirasek
authors.forename: Eva
authors.initials: E
authors.lastname: Hamsikova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001005:Anus Neoplasms / Q000453:epidemiology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology; D003218:Condylomata Acuminata / Q000453:epidemiology / Q000821:virology; D018153:Czech Republic / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D011230:Precancerous Conditions / Q000453:epidemiology / Q000821:virology; D015995:Prevalence; D014846:Vulvar Neoplasms / Q000453:epidemiology / Q000821:virology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology