title: Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques.
abstract: This study presents a selective method of isolation of zearalenone (ZON) and its metabolite, α-zearalenol (α-ZOL), in neoplastically changed human tissue by accelerated solvent and ultrasonic extractions using a mixture of acetonitrile/water (84/16% v/v) as the extraction solvent. Extraction effectiveness was determined through the selection of parameters (composition of the solvent mixture, temperature, pressure, number of cycles) with tissue contamination at the level of nanograms per gram. The produced acetonitrile/water extracts were purified, and analytes were enriched in columns packed with homemade molecularly imprinted polymers. Purified extracts were determined by liquid chromatography (LC) coupled with different detection systems (diode array detection--DAD and mass spectrometry--MS) involving the Ascentis RP-Amide as a stationary phase and gradient elution. The combination of UE-MISPE-LC (ultrasonic extraction--molecularly imprinted solid-phase extraction--liquid chromatography) produced high (R≈95-98%) and repeatable (RSD<3%) recovery values for ZON and α-ZOL.
authors.affiliation: Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin St, 87 100 Toruń, Poland. rgadz@chem.uni.torun.pl
authors.forename: Renata
authors.initials: R
authors.lastname: Gadzała-Kopciuch
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Cendrowski
authors.forename: Anna
authors.initials: A
authors.lastname: Cesarz
authors.forename: Paweł
authors.initials: P
authors.lastname: Kiełbasa
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000368:Aged; D002853:Chromatography, Liquid; D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D011108:Polymers / Q000737:chemistry / Q000378:metabolism; D052616:Solid Phase Extraction; D015025:Zearalenone / Q000032:analysis*; D015029:Zeranol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D011108:Polymers; C029659:zearalenol; D015025:Zearalenone; D015029:Zeranol