title: Bioinformatics analysis for structure and function of CPR of Plasmodium falciparum.
abstract: OBJECTIVE
  To analyse the structure and function of NADPH-cytochrome p450 reductase (CYPOR or CPR) from Plasmodium falciparum (Pf), and to predict its' drug target and vaccine target.
  
  
  METHODS
  The structure, function, drug target and vaccine target of CPR from Plasmodium falciparum were analyzed and predicted by bioinformatics methods.
  
  
  RESULTS
  PfCPR, which was older CPR, had close relationship with the CPR from other Plasmodium species, but it was distant from its hosts, such as Homo sapiens and Anopheles. PfCPR was located in the cellular nucleus of Plasmodium falciparum. 335aa-352aa and 591aa - 608aa were inserted the interior side of the nuclear membrane, while 151aa-265aa was located in the nucleolus organizer regions. PfCPR had 40 function sites and 44 protein-protein binding sites in amino acid sequence. The teriary structure of 1aa-700aa was forcep-shaped with wings. 15 segments of PfCPR had no homology with Homo sapien CPR and most were exposed on the surface of the protein. These segments had 25 protein-protein binding sites. While 13 other segments all possessed function sites.
  
  
  CONCLUSIONS
  The evolution or genesis of Plasmodium falciparum is earlier than those of Homo sapiens. PfCPR is a possible resistance site of antimalarial drug and may involve immune evasion, which is associated with parasite of sporozoite in hepatocytes. PfCPR is unsuitable as vaccine target, but it has at least 13 ideal drug targets.
authors.affiliation: School of Troptical and Laboratory Medicine, Hainan Medical University, Haikou 571101, China. yuyanghoutu2006@yahoo.com.cn
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Fan
authors.forename: Lingmin
authors.initials: L
authors.lastname: Zhang
authors.forename: Guogang
authors.initials: G
authors.lastname: Yan
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Gan
authors.forename: Saifeng
authors.initials: S
authors.lastname: Zhong
authors.forename: Guifen
authors.initials: G
authors.lastname: Lin
mesh_terms: D001665:Binding Sites; D002467:Cell Nucleus / Q000737:chemistry; D019295:Computational Biology / Q000379:methods*; D019143:Evolution, Molecular; D006801:Humans; D017780:Malaria Vaccines / Q000235:genetics / Q000276:immunology; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D010802:Phylogeny; D010963:Plasmodium falciparum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics* / Q000276:immunology; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid
chemical_list: D017780:Malaria Vaccines; D009251:NADPH-Ferrihemoprotein Reductase