title: Polymorphism of Beauveria bassiana (Deuteromycota: Hyphomycetes) strains isolated from Ixodes ricinus (Acari: Ixodidae) in Moldova.
abstract: Polymorphism of 10 Beauveria bassiana strains, isolated from Ixodes ricinus in Moldova, was evaluated using traditional (morphological and cultural properties) and molecular (RAPD patterns and ITS sequences) methods. The isolates differed greatly in morphological and cultural features, such as color, consistence, and growth rate. Four RAPD-PCR markers were used to evaluate genetic diversity of the strains. Phylogenetic neighbor-joining analysis of RAPD patterns divided strains into 3 major clades. The ITS sequences of 8 strains were identical to those of known B. bassiana strains. Two subsets (1 and 2) different by one nucleotide change were found in the ITS1 region. One strain of subset 1 was different from known B. bassiana strains by possessing 2 point mutations in the ITS region. RAPD-based clustering correlated to ITS sequence and colony morphology-based grouping of the strains.
authors.affiliation: All-Russian Institute for Plant Protection, 196608, Podbelskogo 3, St. Petersburg, Russia. galmit@rambler.ru
authors.forename: G V
authors.initials: GV
authors.lastname: Mitina
authors.forename: Y S
authors.initials: YS
authors.lastname: Tokarev
authors.forename: A A
authors.initials: AA
authors.lastname: Movila
authors.forename: T
authors.initials: T
authors.lastname: Yli-Mattila
mesh_terms: D000818:Animals; D052982:Beauveria / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D018884:Ixodes / Q000382:microbiology*; D008966:Moldova; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic