title: Human osteoblast cytotoxicity study of electrospun polyurethane/calcium chloride ultrafine nanofibers.
abstract: This work was focused on preparation and characterization of the polyurethane nanofibers containing calcium chloride (CaCl2) prepared via electrospinning process for the bionanotechnological applications. The morphological, structural characterizations and thermal properties of the polyurethane/CaCl2 nanofibers were determined by using scanning electron microscopy (SEM), field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform-infrared (FT-IR) spectroscopy and thermogravimetry (TGA). SEM images revealed that these composite nanofibers were well-oriented and had good incorporation of CaCl2. The morphological feature of the cells attached on polyurethane/CaCl2 nanofibers scaffold was confirmed by SEM. The in vitro cell compatibility of the polyurethane/CaCl2 composite nanofibers was studied. This study demonstrated the non-toxic effect of electrospun polyurethane/CaCl2 composite nanofibers. Based on this, we propose a mechanism for the cell attachment.
authors.affiliation: Bio-Nano System Engineering, College of Engineering, Chonbuk National University, Jeonju 561 756, South Korea.
authors.forename: R
authors.initials: R
authors.lastname: Nirmala
authors.forename: Hyung-Sub
authors.initials: HS
authors.lastname: Kang
authors.forename: Mohamed H
authors.initials: MH
authors.lastname: El-Newehy
authors.forename: R
authors.initials: R
authors.lastname: Navamathavan
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Park
authors.forename: Hak Yong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D000704:Analysis of Variance; D002122:Calcium Chloride / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D055664:Electrochemical Techniques; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D011140:Polyurethanes / Q000737:chemistry* / Q000494:pharmacology; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011140:Polyurethanes; D002122:Calcium Chloride