title: Laboratory soft-x-ray microscope for cryotomography of biological specimens.
abstract: Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a λ=2.48 nm liquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30 nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to ∼100 nm.
authors.affiliation: Biomedical and X-Ray Physics, Department of Applied Physics, KTH Royal Institute of Technology/AlbaNova, 10691 Stockholm, Sweden.
authors.forename: Michael
authors.initials: M
authors.lastname: Bertilson
authors.forename: Olov
authors.initials: O
authors.lastname: von Hofsten
authors.forename: Ulrich
authors.initials: U
authors.lastname: Vogt
authors.forename: Anders
authors.initials: A
authors.lastname: Holmberg
authors.forename: Athanasia E
authors.initials: AE
authors.lastname: Christakou
authors.forename: Hans M
authors.initials: HM
authors.lastname: Hertz
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology; D057809:HEK293 Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D007753:Laboratories; D008853:Microscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D014054:Tomography / Q000379:methods*; D014965:X-Rays