title: The Candida albicans GAP gene family encodes permeases involved in general and specific amino acid uptake and sensing.
abstract: The Saccharomyces cerevisiae general amino acid permease Gap1 (ScGap1) not only mediates the uptake of most amino acids but also functions as a receptor for the activation of protein kinase A (PKA). Fungal pathogens can colonize different niches in the host, each containing various levels of different amino acids and sugars. The Candida albicans genome contains six genes homologous to the S. cerevisiae GAP1. The expression of these six genes in S. cerevisiae showed that the products of all six C. albicans genes differ in their transport capacities. C. albicans Gap2 (CaGap2) is the true orthologue of ScGap1 as it transports all tested amino acids. The other CaGap proteins have narrower substrate specificities though CaGap1 and CaGap6 transport several structurally unrelated amino acids. CaGap1, CaGap2, and CaGap6 also function as sensors. Upon detecting some amino acids, e.g., methionine, they are involved in a rapid activation of trehalase, a downstream target of PKA. Our data show that CaGAP genes can be functionally expressed in S. cerevisiae and that CaGap permeases communicate to the intracellular signal transduction pathway similarly to ScGap1.
authors.affiliation: Department of Membrane Transport, Institute of Physiology AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Kraidlova
authors.forename: Griet
authors.initials: G
authors.lastname: Van Zeebroeck
authors.forename: Patrick
authors.initials: P
authors.lastname: Van Dijck
authors.forename: Hana
authors.initials: H
authors.lastname: Sychrová
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D026905:Amino Acid Transport Systems; C491145:GAP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D017868:Cyclic AMP-Dependent Protein Kinases