title: Expression characterization and the promoter activity analysis of zebrafish hdac4.
abstract: Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Zhu
authors.forename: Huanling
authors.initials: H
authors.lastname: Wang
authors.forename: Yasmeen
authors.initials: Y
authors.lastname: Gul
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhao
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Shasha
authors.initials: S
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D006224:Cricetinae; D047108:Embryonic Development / Q000187:drug effects; D029941:Fish Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D064326:MEF2 Transcription Factors; D051379:Mice; D008969:Molecular Sequence Data; D018006:Myogenic Regulatory Factors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D041681:NIH 3T3 Cells; D011401:Promoter Regions, Genetic; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D064326:MEF2 Transcription Factors; D018006:Myogenic Regulatory Factors; D029961:Zebrafish Proteins; C012589:trichostatin A; C576359:HDAC4 protein, zebrafish; D006655:Histone Deacetylases