title: Chloroplast-located BjFer1 together with anti-oxidative genes alleviate hydrogen peroxide and hydroxyl radical injury in cytoplasmic male-sterile Brassica juncea.
abstract: We studied how plant cell modulated redox homeostasis in cytoplasmic male-sterility (CMS) Brassica juncea. The CMS Brassica juncea was identified to be mutated in several mitochondrial genes that suggested the changes of cell redox homeostasis. We observed that it was not associated with increased oxidative stress as shown by decreased H(2)O(2) and (∙)OH contents in this type of CMS. The expressions of several anti-oxidative genes were up-regulated in 5-day-old seedlings of CMS than MF lines under light and dark conditions. The mitochondrial alternative oxidase pathway was not activated, as indicated by no increased expression of AOX1a gene in CMS. Interestingly, the expression of Ferritin1 gene was markedly activated in 5-day-old seedlings of CMS than MF line under light and dark conditions. Consequently, we detected increased content of total iron in 30-day-old leaves in CMS than MF line. We isolated Ferritin1 orthologous gene from Brassica juncea, which was targeted to the chloroplast and induced by Fe-citrate and H(2)O(2), not ABA. Taken together, we proposed that increased expressions of BjFer1 and several antioxidant genes protected cell from oxidative stress in CMS Brassica juncea.
authors.affiliation: Laboratory of Genetic Resources & Functional Improvement for Horticultural Plants, Department of Horticulture, Zhejiang University, Hangzhou, 310029, People's Republic of China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Yang
authors.forename: Mingfang
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005293:Ferritins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity*; D017665:Hydroxyl Radical / Q000378:metabolism / Q000633:toxicity*; D007501:Iron / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D009149:Mustard Plant / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010088:Oxidoreductases / Q000378:metabolism; D051479:Plant Infertility / Q000187:drug effects* / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D000099188:Alternative Oxidase
chemical_list: D000975:Antioxidants; D005293:Ferritins; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical; D007501:Iron; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D000099188:Alternative Oxidase