title: Identification of integrin beta subunit mutations that alter affinity for extracellular matrix ligand.
abstract: We examined over 50 mutations in the Drosophila βPS integrin subunit that alter integrin function in situ for their ability to bind a soluble monovalent ligand, TWOW-1. Surprisingly, very few of the mutations, which were selected for conditional lethality in the fly, reduce the ligand binding ability of the integrin. The most prevalent class of mutations activates the integrin heterodimer. These findings emphasize the importance of integrin affinity regulation and point out how molecular interactions throughout the integrin molecule are important in keeping the integrin in a low affinity state. Mutations strongly support the controversial deadbolt hypothesis, where the CD loop in the β tail domain acts to restrain the I domain in the inactive, bent conformation. Site-directed mutations in the cytoplasmic domains of βPS and αPS2C reveal different effects on ligand binding from those observed for αIIbβ3 integrins and identify for the first time a cytoplasmic cysteine residue, conserved in three human integrins, as being important in affinity regulation. In the fly, we find that genetic interactions of the βPS mutations with reduction in talin function are consistent with the integrin affinity differences measured in cells. Additionally, these genetic interactions report on increased and decreased integrin functions that do not result in affinity changes in the PS2C integrin measured in cultured cells.
authors.affiliation: Department of Molecular and Cellular Biology, Arizona Cancer Center, Tucson, Arizona 85724.
authors.forename: Timmy
authors.initials: T
authors.lastname: Kendall
authors.affiliation: Department of Molecular and Cellular Biology, Arizona Cancer Center, Tucson, Arizona 85724.
authors.forename: Leona
authors.initials: L
authors.lastname: Mukai
authors.affiliation: Department of Molecular and Cellular Biology, Arizona Cancer Center, Tucson, Arizona 85724.
authors.forename: Alison L
authors.initials: AL
authors.lastname: Jannuzi
authors.affiliation: Department of Molecular and Cellular Biology, Arizona Cancer Center, Tucson, Arizona 85724. Electronic address: tbunch@email.arizona.edu.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Bunch
mesh_terms: D000483:Alleles; D000818:Animals; D002448:Cell Adhesion; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D039001:Integrin alpha Chains / Q000235:genetics*; D039641:Integrin beta Chains / Q000235:genetics*; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D016608:Talin / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D008024:Ligands; D011506:Proteins; D016608:Talin; C082188:if protein, Drosophila