title: Lateral flow assay for simultaneous detection of cellular- and humoral immune responses.
abstract: OBJECTIVE
  The development of a cytokine detection assay suitable for detection of multiple biomarkers for improved diagnosis of mycobacterial diseases.
  
  
  METHODS
  A lateral flow (LF) assay to detect IL-10 was developed utilizing the up-converting phosphor (UCP) reporter-technology. The assay was evaluated using blood samples of leprosy patients. Multiplex applications were explored targeting: 1) IL-10 and IFN-γ in assay buffer; 2) IL-10 and anti-phenolic glycolipid (PGL-I) antibodies in serum from leprosy patients.
  
  
  RESULTS
  Detection of IL-10 below the targeted level of 100pg/mL in serum was shown. Comparison with ELISA showed a quantitative correlation with R(2) value of 0.92. Multiplexing of cytokines and simultaneous detection of cytokine and antibody was demonstrated.
  
  
  CONCLUSIONS
  The UCP-LF IL-10 assay is a user-friendly, rapid alternative for IL-10 ELISAs, suitable for multiplex detection of different cytokines and can be merged with antibody-detection assays to simultaneously detect cellular- and humoral immunity.
authors.affiliation: Department of Molecular Cell Biology Leiden University Medical Center, Einthovenweg 20, 2333 ZC Leiden, The Netherlands. P.Corstjens@LUMC.NL
authors.forename: Paul L A M
authors.initials: PL
authors.lastname: Corstjens
authors.forename: Claudia J
authors.initials: CJ
authors.lastname: de Dood
authors.forename: Jolien J
authors.initials: JJ
authors.lastname: van der Ploeg-van Schip
authors.forename: Karien C
authors.initials: KC
authors.lastname: Wiesmeijer
authors.forename: Terhi
authors.initials: T
authors.lastname: Riuttamäki
authors.forename: Krista E
authors.initials: KE
authors.lastname: van Meijgaarden
authors.forename: John S
authors.initials: JS
authors.lastname: Spencer
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: Tom H M
authors.initials: TH
authors.lastname: Ottenhoff
authors.forename: Annemieke
authors.initials: A
authors.lastname: Geluk
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D001681:Biological Assay / Q000379:methods*; D015415:Biomarkers / Q000097:blood; D002021:Buffers; D016207:Cytokines / Q000097:blood* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis; D006801:Humans; D056724:Immunity, Humoral; D007371:Interferon-gamma / Q000032:analysis* / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood*; D007918:Leprosy / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000276:immunology; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D002021:Buffers; D016207:Cytokines; D006017:Glycolipids; C508609:IL10 protein, human; C045169:phenolic glycolipid I, Mycobacterium leprae; D016753:Interleukin-10; D007371:Interferon-gamma