title: Rapid discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by use of a singleplex PCR.
abstract: We report here a PCR-based assay using a single primer pair targeting the RPL31 gene that allows discrimination between Candida glabrata, Candida bracarensis, and Candida nivariensis according to the size of the generated amplicon.
authors.affiliation: APHP, Hôpital Bicêtre, Service de Bactériologie-Virologie-Parasitologie, Laboratoire de Parasitologie-Mycologie, Kremlin-Bicêtre 94275, France. adela.angoulvant@bct.aphp.fr
authors.forename: A
authors.initials: A
authors.lastname: Enache-Angoulvant
authors.forename: J
authors.initials: J
authors.lastname: Guitard
authors.forename: F
authors.initials: F
authors.lastname: Grenouillet
authors.forename: T
authors.initials: T
authors.lastname: Martin
authors.forename: P
authors.initials: P
authors.lastname: Durrens
authors.forename: C
authors.initials: C
authors.lastname: Fairhead
authors.forename: C
authors.initials: C
authors.lastname: Hennequin
mesh_terms: D001483:Base Sequence; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer