title: In vitro tetraploid induction from leaf explants of Populus pseudo-simonii Kitag.
abstract: Tetraploid plants were produced from leaf explants of diploid Populus pseudo-simonii by treating the leaves with colchicine. Leaf explants were cultured on MS basal medium containing 1.78 μM BA and 1.08 μM NAA for 0, 6 and 12 days, and then transferred to the same MS liquid medium with colchicine at concentrations of 25, 50 and 75 μM for 1, 2 and 3 days. The highest efficiency of tetraploid induction was 14.6% by treating leaf explants that were pre-cultured for 6 days and then cultured in liquid MS with 50 μM colchicine for 3 days. Flow cytometric analysis was used to screen the tetraploids out from the regenerated plants and chromosome number counting was employed to confirm the polyploidy level. Size and frequency of leaf stomata between diploid and tetraploid plants were demonstrated to have significant differences.
authors.affiliation: Key National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 100083, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cai
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Kang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D003583:Cytokinins / Q000494:pharmacology; D005434:Flow Cytometry; D007210:Indoleacetic Acids / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D054046:Plant Stomata / Q000254:growth & development / Q000502:physiology; D032107:Populus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D012038:Regeneration; D057891:Tetraploidy
chemical_list: D003470:Culture Media; D003583:Cytokinins; D007210:Indoleacetic Acids; D003078:Colchicine