title: Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.
abstract: Capturing the right ligand at the right spot: a well-balanced system for non-natural amino acid mutagenesis allows the ligand binding sites of a class II G-protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.
authors.affiliation: Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
authors.forename: Irene
authors.initials: I
authors.lastname: Coin
authors.forename: Marilyn H
authors.initials: MH
authors.lastname: Perrin
authors.forename: Wylie W
authors.initials: WW
authors.lastname: Vale
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D057809:HEK293 Cells; D006801:Humans; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D019920:Photoaffinity Labels; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D008024:Ligands; D019920:Photoaffinity Labels; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins