title: In vivo imaging and spectroscopy of dynamic metabolism using simultaneous 13C and 1H MRI.
abstract: Hyperpolarized (HP) (13)C-labeled pyruvate studies with magnetic resonance (MR) have been used to observe the kinetics of metabolism in vivo. Kinetic modeling to measure metabolic rates in vivo is currently limited because of nonspecific hyperpolarized signals mixing between vascular, extravascular, and intracellular compartments. In this study, simultaneous acquisition of both (1)H and (13)C signals after contrast agent injection is used to resolve specific compartments to improve the accuracy of the modeling. We demonstrate a novel technique to provide contrast to the intracellular compartments by sequential injection of HP [1-(13)C] pyruvate followed by gadolinium-chelate to provide T(1)-shortening to extra-cellular compartments. A kinetic model that distinguishes the intracellular space and includes the T(1)-shortening effect of the gadolinium chelate can then be used to directly measure the intracellular (13)C kinetics.
authors.affiliation: Department of Medical Physics, University of Wisconsin, Madison, WI 53705, USA. mrsmith4@wisc.edu
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Smith
authors.forename: Eric T
authors.initials: ET
authors.lastname: Peterson
authors.forename: Jeremy W
authors.initials: JW
authors.lastname: Gordon
authors.forename: David J
authors.initials: DJ
authors.lastname: Niles
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Rowland
authors.forename: Krishna N
authors.initials: KN
authors.lastname: Kurpad
authors.forename: Sean B
authors.initials: SB
authors.lastname: Fain
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D002250:Carbon Radioisotopes; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008657:Metabolic Clearance Rate; D011522:Protons; D019289:Pyruvic Acid / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002250:Carbon Radioisotopes; D011522:Protons; D019289:Pyruvic Acid