title: [The role of brain-derived neurotrophic factor in pain facilitation and spinal mechanism in rat model of bone cancer pain].
abstract: OBJECTIVE
  To investigate the role of brain-derived neurotrophic factor (BDNF) in pain facilitation and spinal mechanisms in the rat model of bone cancer pain.
  
  
  METHODS
  The bone cancer pain model was developed by inoculated Walker 256 mammary gland carcinoma cells into the tibia medullary cavity. Sixty SD female rats were divided into 5 groups (n = 12 each) randomly; group I: control group (sham operation); group II: model group; group III: control group + anti-BDNF intrathecal (i.t.); group IV: model group + control IgG i.t.; group V: model group + anti-BDNF i.t.. Anti-BDNF or control IgG was injected i.t. during 7 to 9th day. Von-Frey threshold was measured one day before operation and every 2 days after operation. On the 9th day after threshold tested, rats were sacrificed after i.t. injection of either anti-BDNF or control IgG, the lumbar 4-6 spinal cord was removed. The expression of the spinal BDNF and the phosphorylation of extracellular signal-regulated protein kinase 1/2 (p-ERK1/2) were detected by immunohistochemistry assay and Western-Blot. Co-expression pattern of BDNF and p-ERK1/2 were determined by double-labeling immunofluorescence.
  
  
  RESULTS
  We demonstrated the coexistence of BDNF and p-ERK1/2 in the spinal cord of rats. From the 7 to 9th day after operation, von-Frey threshold in groups II and IV was significantly lower than that in group I and group V (P < 0.01), group V was remarkly higher than that in group IV (P < 0.01). The spinal BDNF and p-ERK1/2 expression in group II or IV were significantly increased compared with that in group I or V (P < 0.01), intrathecal anti-BDNF was significantly suppressed BDNF and p-ERK1/2 expression (P < 0.01).
  
  
  CONCLUSIONS
  BDNF and p-ERK1/2 was coexistence in the spinal cord of rats, and it maybe involved in the bone cancer pain.
authors.affiliation: Department of Anesthesiology, the First Hospital Affiliated to Suzhou University, Suzhou 215006, China.
authors.forename: Li-na
authors.initials: LN
authors.lastname: Wang
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Yang
authors.forename: Fu-hai
authors.initials: FH
authors.lastname: Ji
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: Wang
authors.forename: Jian-ling
authors.initials: JL
authors.lastname: Zuo
authors.forename: Qi-nian
authors.initials: QN
authors.lastname: Xu
authors.forename: Xiao-ming
authors.initials: XM
authors.lastname: Jia
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Chun-guang
authors.initials: CG
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000150:complications / Q000378:metabolism*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002279:Carcinoma 256, Walker / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism; D020935:MAP Kinase Signaling System; D010146:Pain / Q000209:etiology / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor