title: Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice.
abstract: OBJECTIVE
  The aim of this study is to investigate the synergistic effect of chondroitinase ABC and growth factors in the integration of murine retinal progenitor cells (mRPCs) transplanted into Rho(-/-) mice.
  
  
  METHODS
  mRPCs from P1 green fluorescent protein-transgenic mice were isolated and expanded for transplantation. All mRPCs of 20 passages or less were transplanted into the subretinal space of B6 mice together with chondroitinase ABC, and into Rho(-/-) mice combined with chondroitinase ABC, N-[N-(3, 5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), and insulin growth factor (IGF)-1. Cell counts were used to examine the migration and survival rate of mRPCs in B6 mice. Immunohistochemistry was used to evaluate the differentiation and integration of mRPCs in B6 and Rho(-/-) mice.
  
  
  RESULTS
  Our results show that substantial numbers of mRPCs migrated and survived in the retina when transplanted with chondroitinase ABC into B6 and Rho(-/-) mice. Chondroitinase ABC disrupted the glial scar around the mRPCs in the subretinal space. Only a few mRPCs expressed recoverin in B6 mice. More mRPCs expressed rhodopsin, recoverin, and synaptophysin after transplantation into Rho(-/-) mice when combined with chondroitinase ABC and growth factors.
  
  
  CONCLUSIONS
  The synergistic effect of chondroitinase ABC and growth factors facilitates the anatomic integration of mRPCs transplanted into Rho(-/-) mice.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Mostafa
authors.initials: M
authors.lastname: Kabiel
authors.forename: Budd A
authors.initials: BA
authors.lastname: Tucker
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Young
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D019765:Chondroitin ABC Lyase / Q000008:administration & dosage*; D004151:Dipeptides / Q000008:administration & dosage*; D004338:Drug Combinations; D004357:Drug Synergism; D017930:Genes, Reporter; D006085:Graft Survival / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000637:transplantation; D012243:Rhodopsin / Q000172:deficiency* / Q000235:genetics; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D004151:Dipeptides; D004338:Drug Combinations; C419410:N-(N-(3,5-difluorophenacetyl)alanyl)phenylglycine tert-butyl ester; D049452:Green Fluorescent Proteins; D007334:Insulin-Like Growth Factor I; D012243:Rhodopsin; D019765:Chondroitin ABC Lyase