title: Carbon disulfide inhibits neurite outgrowth and neuronal migration of dorsal root ganglion in vitro.
abstract: Carbon disulfide (CS₂) is a neurotoxic industrial solvent and widely used in the vulcanization of rubber, rayon, cellophane, and adhesives. Although the neurotoxicity of CS₂ has been recognized for over a century, the precise mechanism of neurotoxic action of CS₂ remains unknown. In the present study, a embryonic rat dorsal root ganglia (DRG) explants culture model was established. Using the organotypic DRG cultures, the direct neurotoxic effects of CS₂ on outgrowth of neurites and migration of neurons from DRG explants were investigated. The organotypic DRG cultures were exposed to different concentrations of CS₂ (0.01 mmol/L, 0.1 mmol/L, 1 mmol/L). The number of nerve fiber bundles extended from DRG explants decreased significantly in the presence of CS₂ (0.01 mmol/L, 15.00 ± 2.61, p < .05; 0.1 mmol/L, 11.17 ± 1.47, p < .001; 1 mmol/L, 8.00 ± 1.41, p < .001) as compared with that in the absence of CS₂ (17.83 ± 2.48). The number of neurons migrated from DRG explants decreased significantly in the presence of CS₂ (0.01 mmol/L, 79.50 ± 9.40, p < .01; 0.1 mmol/L, 62.50 ± 14.15, p < .001; 1 mmol/L, 34.67 ± 7.58, p < .001) as compared with that in the absence of CS₂ (99.33 ± 15.16). And also, the decreases in the number of nerve fiber bundles and migrated DRG neurons were in a dose-dependent manner of CS₂. These data implicated that CS₂ could inhibit neurite outgrowth and neuronal migration from DRG explants in vitro.
authors.affiliation: Shandong University's School of Public Health, Jinan, Shandong Province, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Xiang
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhang
authors.forename: Huaxiang
authors.initials: H
authors.lastname: Liu
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D002246:Carbon Disulfide / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000254:growth & development*; D009433:Neural Inhibition / Q000187:drug effects* / Q000502:physiology; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002246:Carbon Disulfide