title: A moderate aseptic local inflammation does not induce a significant systemic inflammatory response.
abstract: The aim of this prospective study was to characterize the response of the coagulation system to a defined sterile localized inflammatory process. Tissue cages were implanted subcutaneously in five healthy Beagles. After 9-10 weeks, local inflammation was induced by an injection of 0.5 ml 1% carrageenan. Serial samples of tissue cage fluid (TCF) and blood were collected at 10 time points (0-168 h). Nucleated cells (NC) of TCF were counted automatically to characterize local inflammation. C-reactive protein (CRP), leukocytes and coagulation variables (PT, aPTT, fibrinogen, factor VIII, antithrombin, protein C, protein S, and d-dimers) were determined in blood samples. Carrageenan induced a significant 32-fold increase of NCs in TCF (P<0.0001). A slight increase in leukocytes (P<0.0001) was observed. There was a significant 1.3- to 1.5-fold increase in protein C (P=0.0001) and protein S (P=0.0028). CRP, secondary hemostasis and fibrinolysis did not change. The mild increase from baseline in PC/PS, may reflect a physiological counter reaction.
authors.affiliation: Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology, Justus-Liebig-University Giessen, Giessen, Germany. natali.bauer@vetmed.uni-giessen.de
authors.forename: Natali
authors.initials: N
authors.lastname: Bauer
authors.forename: Sandra
authors.initials: S
authors.lastname: Mensinger
authors.forename: Gert
authors.initials: G
authors.lastname: Daube
authors.forename: Klaus
authors.initials: K
authors.lastname: Failing
authors.forename: Andreas
authors.initials: A
authors.lastname: Moritz
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis; D002351:Carrageenan / Q000008:administration & dosage / Q000494:pharmacology; D015194:Diffusion Chambers, Culture / Q000662:veterinary; D004283:Dog Diseases / Q000097:blood / Q000139:chemically induced / Q000473:pathology*; D004285:Dogs; D005169:Factor VIII / Q000032:analysis; D005260:Female; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D007279:Injections, Subcutaneous / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D011486:Protein C / Q000032:analysis; D017293:Protein S / Q000032:analysis
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D011486:Protein C; D017293:Protein S; C036309:fibrin fragment D; D002351:Carrageenan; D005169:Factor VIII; D005340:Fibrinogen; D002097:C-Reactive Protein