title: Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey.
abstract: Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical areas. We investigated the presence and distribution of Anaplasma spp. in cattle from 6 provinces in Turkey. For amplification of the segment spanning the V1 region of the 16S ribosomal RNA (rRNA) gene of Anaplasma species, a reverse line blot (RLB) hybridization assay was performed on 389 blood samples. RLB identified Anaplasma infections in 9.0% (35/389) of the samples. The most frequently found species was A. marginale (11/389, 2.8%), followed by A. centrale (4/389, 1.0%) and A. phagocytophilum (4/389, 1.0%). Eighteen of 35 PCR-positive samples gave positive signals to the catch-all probes, but did not show any response to the species-specific probes tested. Sequencing results of 5 representative amplicons randomly selected from these specimens indicated that 3 were 100% identical to the sequence of A. ovis, and the other 2 sequences were 99.5% identical to the sequence of Ehrlichia sp. Omatjenne strain. The results further confirmed that A. ovis and Ehrlichia sp. Omatjenne infection occurs in cattle populations in Turkey.
authors.affiliation: Dept. of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey. maktas@firat.edu.tr
authors.forename: Munir
authors.initials: M
authors.lastname: Aktas
authors.forename: Kursat
authors.initials: K
authors.lastname: Altay
authors.forename: Nazir
authors.initials: N
authors.lastname: Dumanli
mesh_terms: D000709:Anaplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology; D004536:Ehrlichia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D014421:Turkey / Q000453:epidemiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S