title: Nasal allergen provocation test with multiple aeroallergens detects polysensitization in local allergic rhinitis.
abstract: BACKGROUND
  Patients previously given a diagnosis of nonallergic rhinitis (NAR) might have a new form of local allergic rhinitis (LAR) with local production of specific IgE antibodies and a positive response to a nasal allergen provocation test (NAPT).
  
  
  OBJECTIVE
  We evaluated an NAPT protocol using multiple aeroallergens (NAPT-M) for the detection of polysensitization to several aeroallergens in patients with LAR.
  
  
  METHODS
  NAPT-Ms with 2 different panels of aeroallergens for patients with perennial and seasonal rhinitis were performed in 25 patients with LAR and 25 patients with NAR whose disease was diagnosed by means of NAPTs 1 year earlier. The response to nasal challenge was evaluated based on subjective (nasal-ocular symptoms) and objective (acoustic rhinometry) parameters. Nasal levels of tryptase and eosinophil cationic protein were determined by means of immunoassay at baseline, 15 minutes, and 1, 2, and 24 hours after challenge.
  
  
  RESULTS
  NAPT-Ms showed 100% concordance with the gold standard of NAPTs with a single aeroallergen. No false-positive or false-negative responses were detected. The use of NAPT-Ms achieved 75% reduction in the total number of visits required for final diagnosis in the NAR group (from 168 to 42 visits) and a 55% reduction in the LAR group (from 144 to 65 visits) compared with NAPTs with a single aeroallergen.
  
  
  CONCLUSIONS
  These results demonstrate that clinically relevant polysensitization to aeroallergens in patients with LAR occurred and that the NAPT-M is a useful, specific, sensitive, reproducible, and less time-consuming in vivo diagnostic test for the screening of patients with LAR.
authors.affiliation: Allergy Service, Carlos Haya Hospital, Malaga, Spain. carmenrs61@gmail.com
authors.forename: Carmen
authors.initials: C
authors.lastname: Rondón
authors.forename: Paloma
authors.initials: P
authors.lastname: Campo
authors.forename: Rocío
authors.initials: R
authors.lastname: Herrera
authors.forename: Natalia
authors.initials: N
authors.lastname: Blanca-Lopez
authors.forename: Lidia
authors.initials: L
authors.lastname: Melendez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Canto
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Torres
authors.forename: Miguel
authors.initials: M
authors.lastname: Blanca
mesh_terms: D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000009:adverse effects / Q000276:immunology; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology; D009299:Nasal Provocation Tests / Q000379:methods*; D012220:Rhinitis / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D000485:Allergens