title: Comparative Proteomic Analysis of saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield.
abstract: BACKGROUND
  Saccharopolyspora spinosa produces the environment-friendly biopesticide spinosad, a mixture of two polyketide-derived macrolide active ingredients called spinosyns A and D. Therefore considerable interest is in the improvement of spinosad production because of its low yield in wild-type S. spinosa. Recently, a spinosad-hyperproducing PR2 strain with stable heredity was obtained from protoplast regeneration of the wild-type S. spinosa SP06081 strain. A comparative proteomic analysis was performed on the two strains during the first rapid growth phase (RG1) in seed medium (SM) by using label-free quantitative proteomics to investigate the underlying mechanism leading to the enhancement of spinosad yield.
  
  
  RESULTS
  In total, 224 proteins from the SP06081 strain and 204 proteins from the PR2 strain were unambiguously identified by liquid chromatography-tandem mass spectrometry analysis, sharing 140 proteins. A total of 12 proteins directly related to spinosad biosynthesis were identified from the two strains in RG1. Comparative analysis of the shared proteins revealed that approximately 31% of them changed their abundance significantly and fell in all of the functional groups, such as tricarboxylic acid cycles, glycolysis, biosynthetic processes, catabolic processes, transcription, translation, oxidation and reduction. Several key enzymes involved in the synthesis of primary metabolic intermediates used as precursors for spinosad production, energy supply, polyketide chain assembly, deoxysugar methylation, and antioxidative stress were differentially expressed in the same pattern of facilitating spinosad production by the PR2 strain. Real-time reverse transcriptase polymerase chain reaction analysis revealed that four of five selected genes showed a positive correlation between changes at the translational and transcriptional expression level, which further confirmed the proteomic analysis.
  
  
  CONCLUSIONS
  The present study is the first comprehensive and comparative proteome analysis of S. spinosa strains. Our results highlight the differentially expressed proteins between the two S. spinosa strains and provide some clues to understand the molecular and metabolic mechanisms that could lead to the increased spinosad production yield.
authors.affiliation: Hunan Provincial Key Laboratory of Microbial Molecular Biology--State Key Laboratory of Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, P, R, China. xialq@hunnu.edu.cn.
authors.forename: Yushuang
authors.initials: Y
authors.lastname: Luo
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Ding
authors.forename: Liqiu
authors.initials: L
authors.lastname: Xia
authors.forename: Fan
authors.initials: F
authors.lastname: Huang
authors.forename: Wenping
authors.initials: W
authors.lastname: Li
authors.forename: Shaoya
authors.initials: S
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Sun