title: In vitro activities of acetonic extracts from leaves of three forage legumes (Calliandra calotyrsus, Gliricidia sepium and Leucaena diversifolia) on Haemonchus contortus.
abstract: OBJECTIVE
  To assess ovicidal activity of three acetonic extracts from the leaves of three forage legume, Calliandra calotyrsus (C. calotyrsus), Gliricidia sepium (G. sepium) and Leucaena diversifolia (L. diversifolia) in vitro on Haemonchus contortus (H. contortus).
  
  
  METHODS
  Eggs were exposed for 24 hours to five different concentrations (0.075, 0.15, 0.3, 0.6 and 1.25 mg/mL) of acetonic extracts at room temperature (24 °C). Distilled water and 0.4% Tween were used in the bioassay as negative controls.
  
  
  RESULTS
  The later did not affect embryonation and egg hatching of H. contortus. Conversively, significant effects were obtained with the acetonic extracts of leaves of all three plants and the maximum activity was observed with the highest concentration (1.25 mg/mL). The acetonic extract of G. sepium was found to be more active (2.9% and 0.0% for embryonation and egg hatching, respectively) than the other substances 16.5% and 33.5%, respectively for C. calothyrsus, 33.7% and 33.3%, respectively for L. diversifolia.
  
  
  CONCLUSIONS
  These results suggest that the three forage legumes do possess ovicidal properties and further studies on larvae should be carried out.
authors.affiliation: Department of Animal Biology, Faculty of Science, University of Dschang, P O Box 067 Dschang, Cameroon. waboponejosue@yahoo.fr
authors.forename: J
authors.initials: J
authors.lastname: Wabo Poné
authors.forename: Florence
authors.initials: F
authors.lastname: Kenne Tameli
authors.forename: Mbida
authors.initials: M
authors.lastname: Mpoame
authors.forename: E
authors.initials: E
authors.lastname: Pamo Tedonkeng
authors.forename: C F
authors.initials: CF
authors.lastname: Bilong Bilong
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D006189:Haemonchus / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D010936:Plant Extracts