title: A fluorescence lifetime spectroscopy study of matrix metalloproteinases-2 and -9 in human atherosclerotic plaque.
abstract: Matrix metalloproteinase (MMP)-2 and -9 play important roles in the progression of atherosclerosis. This study aims to determine whether MMP-2 and -9 content in the fibrotic caps of atherosclerotic plaque is correlated with plaque autofluorescence. A time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) system was used to measure the autofluorescence and assess the biochemical composition of human plaques obtained from carotid endarterectomy. Results presented here demonstrate for the first time the ability to characterize the biochemical composition as it relates to MMP-2 and -9 content in the atherosclerotic plaque cap using a label-free imaging technique implemented with a fiberoptic TR-LIFS system.
authors.affiliation: University of California Davis, Department of Biomedical Engineering, Davis, CA 95616, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Phipps
authors.forename: Nisa
authors.initials: N
authors.lastname: Hatami
authors.forename: Zorina S
authors.initials: ZS
authors.lastname: Galis
authors.forename: J Dennis
authors.initials: JD
authors.lastname: Baker
authors.forename: Michael C
authors.initials: MC
authors.lastname: Fishbein
authors.forename: Laura
authors.initials: L
authors.lastname: Marcu
mesh_terms: D016894:Endarterectomy, Carotid / Q000379:methods; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000032:analysis* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000032:analysis* / Q000378:metabolism; D058226:Plaque, Atherosclerotic / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9