title: Gene expression profile of the synovium and cartilage in a chronic arthritis rat model.
abstract: BACKGROUND
  Primary osteoarthritis (OA) is a polygenic disease. To investigate the gene expression profile of cartilage and synovium from osteoarthritis and healthy rats using cDNA microarray is beneficial to recognize the pathogenesis of osteoarthritis and provide evidence for gene therapy of osteoarthritis.
  
  
  OBJECTIVE
  The present study aimed to investigate the gene expression profile of the cartilage and synovium of chronic arthritis and healthy rats through cDNA microarray assay, and identify the differentially expressed genes. This study may be helpful for understanding the role of differentially expressed genes in osteoarthritis and the gene polymorphism of osteoarthritis.
  
  
  METHODS
  A total of 24 male Wistar rats were randomly divided into control group and osteoarthritis group (n = 12 per group). The synovial and cartilage were obtained and total RNA was extracted. cDNA microarray assay was performed to identify the differentially expressed genes, and cluster analysis was conducted.
  
  
  CONCLUSIONS
  A total of 82 differentially expressed genes were identified, among which 27 were up-regulated and 55 down-regulated. Gene microarray assay is effective to identify differentially expressed genes and may find out novel osteoarthritis associated genes. Multiple genes are involved in the pathogenesis of osteoarthritis. The differentially expressed genes provide important information for further studies on the pathogenesis of osteoarthritis and gene therapy of osteoarthritis.
authors.affiliation: Department of Sports Medicine, Second People's Hospital of Shenzhen, Shenzhen, Guangdong Province, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhu
authors.forename: Daping
authors.initials: D
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Yun
authors.initials: Y
authors.lastname: Han
authors.forename: Yangkan
authors.initials: Y
authors.lastname: Ou
authors.forename: Ke
authors.initials: K
authors.lastname: Zhou
authors.forename: Liangquan
authors.initials: L
authors.lastname: Peng
authors.forename: Wenzhe
authors.initials: W
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D055808:Drug Discovery; D004734:Energy Metabolism / Q000235:genetics; D048788:Growth and Development / Q000235:genetics; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D008297:Male; D010003:Osteoarthritis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D059467:Transcriptome
chemical_list: D036341:Intercellular Signaling Peptides and Proteins