title: A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody.
abstract: We developed a novel ultrarapid immunohistochemical staining method in which an AC electric field is used to facilitate detection of tumor cells. Frozen sections of non-small cell lung cancer in lymph nodes were fixed in acetone for 2 min, after which they were incubated for 2 min with an anti-pancytokeratin antibody cocktail and then with EnVision(TM) complex under an alternating current (AC) electric field. The sections were then incubated with a chromogen (3,3'diaminobenzidine) for 3 min and counterstained with hematoxylin. This method enabled detection of tumor cells in frozen sections in less than 15 min. In addition, we were able to reduce the amount of antibody used by more than 90% when the sections were incubated under the AC electric field for a longer period. This method could be a useful tool for frozen section diagnosis and research. Furthermore, with this method the cost of immunohistochemical staining can be reduced.
authors.affiliation: Department of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo Akita City 010-8543, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Toda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Minamiya
authors.forename: Masami
authors.initials: M
authors.lastname: Kagaya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nanjo
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Akagami
authors.forename: Hajime
authors.initials: H
authors.lastname: Saito
authors.forename: Manabu
authors.initials: M
authors.lastname: Ito
authors.forename: Hayato
authors.initials: H
authors.lastname: Konno
authors.forename: Satoru
authors.initials: S
authors.lastname: Motoyama
authors.forename: Junichi
authors.initials: J
authors.lastname: Ogawa