title: Isolation of Aggregatibacter aphrophilus from a patient with acute appendicitis.
abstract: OBJECTIVE
  Acute appendicitis is a common surgical emergency. The etiology and pathophysiology of appendicitis have been well investigated. Aggregatibacter aphrophilus is a fastidious gram-negative coccobacilli. Detection of this organism in clinical samples and its differentiation from Haemophilus aphrophilus or from Aggregatibacter actinomycetemcomitans in routine microbiology settings could be difficult.
  
  
  METHODS
  In this rare case, we report the isolation of Aggregatibacter aphrophilus from the appendix of a 14-year-old boy presented with acute appendicitis. The genotypic method using 16S rRNA sequencing was used for identification of the organism at species level.
  
  
  CONCLUSIONS
  This case highlights the importance of detecting fastidious and rare microorganisms such as Aggregatibacter aphrophilus that could be associated with acute appendicitis.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: A M
authors.initials: AM
authors.lastname: Aye
authors.forename: C W
authors.initials: CW
authors.lastname: Law
authors.forename: N S
authors.initials: NS
authors.lastname: Sabet
authors.forename: R
authors.initials: R
authors.lastname: Karunakaran
authors.forename: Y A
authors.initials: YA
authors.lastname: Hanifah
authors.forename: F L
authors.initials: FL
authors.lastname: Jafar
authors.forename: S
authors.initials: S
authors.lastname: Abubakar
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D001062:Appendectomy; D001064:Appendicitis / Q000382:microbiology* / Q000601:surgery; D001065:Appendix / Q000382:microbiology* / Q000601:surgery; D004269:DNA, Bacterial / Q000302:isolation & purification; D044136:Haemophilus paraphrophilus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S