title: Organization of nif gene cluster in Frankia sp. EuIK1 strain, a symbiont of Elaeagnus                      umbellata.
abstract: The nucleotide sequence of a 20.5-kb genomic region harboring nif genes was determined and analyzed. The fragment was obtained from Frankia sp. EuIK1 strain, an indigenous symbiont of Elaeagnus umbellata. A total of 20 ORFs including 12 nif genes were identified and subjected to comparative analysis with the genome sequences of 3 Frankia strains representing diverse host plant specificities. The nucleotide and deduced amino acid sequences showed highest levels of identity with orthologous genes from an Elaeagnus-infecting strain. The gene organization patterns around the nif gene clusters were well conserved among all 4 Frankia strains. However, characteristic features appeared in the location of the nifV gene for each Frankia strain, depending on the type of host plant. Sequence analysis was performed to determine the transcription units and suggested that there could be an independent operon starting from the nifW gene in the EuIK strain. Considering the organization patterns and their total extensions on the genome, we propose that the nif gene clusters remained stable despite genetic variations occurring in the Frankia genomes.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, 151-747, Korea.
authors.forename: Chang Jae
authors.initials: CJ
authors.lastname: Oh
authors.forename: Ho Bang
authors.initials: HB
authors.lastname: Kim
authors.forename: Jitae
authors.initials: J
authors.lastname: Kim
authors.forename: Won Jin
authors.initials: WJ
authors.lastname: Kim
authors.forename: Hyoungseok
authors.initials: H
authors.lastname: Lee
authors.forename: Chung Sun
authors.initials: CS
authors.lastname: An
mesh_terms: D055028:Comparative Genomic Hybridization; D004269:DNA, Bacterial / Q000235:genetics; D031263:Elaeagnaceae / Q000382:microbiology*; D040161:Frankia / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D009586:Nitrogen Fixation / Q000235:genetics; D009591:Nitrogenase / Q000235:genetics*; D016366:Open Reading Frames; D009876:Operon; D017422:Sequence Analysis, DNA; D013559:Symbiosis
chemical_list: D004269:DNA, Bacterial; D009591:Nitrogenase