title: Functional tooth regeneration using a bioengineered tooth unit as a mature organ replacement regenerative therapy.
abstract: Donor organ transplantation is currently an essential therapeutic approach to the replacement of a dysfunctional organ as a result of disease, injury or aging in vivo. Recent progress in the area of regenerative therapy has the potential to lead to bioengineered mature organ replacement in the future. In this proof of concept study, we here report a further development in this regard in which a bioengineered tooth unit comprising mature tooth, periodontal ligament and alveolar bone, was successfully transplanted into a properly-sized bony hole in the alveolar bone through bone integration by recipient bone remodeling in a murine transplantation model system. The bioengineered tooth unit restored enough the alveolar bone in a vertical direction into an extensive bone defect of murine lower jaw. Engrafted bioengineered tooth displayed physiological tooth functions such as mastication, periodontal ligament function for bone remodeling and responsiveness to noxious stimulations. This study thus represents a substantial advance and demonstrates the real potential for bioengineered mature organ replacement as a next generation regenerative therapy.
authors.affiliation: Research Institute for Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Oshima
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Mizuno
authors.forename: Aya
authors.initials: A
authors.lastname: Imamura
authors.forename: Miho
authors.initials: M
authors.lastname: Ogawa
authors.forename: Masato
authors.initials: M
authors.lastname: Yasukawa
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Morita
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ikeda
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakao
authors.forename: Teruko
authors.initials: T
authors.lastname: Takano-Yamamoto
authors.forename: Shohei
authors.initials: S
authors.lastname: Kasugai
authors.forename: Masahiro
authors.initials: M
authors.lastname: Saito
authors.forename: Takashi
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D016301:Alveolar Bone Loss / Q000503:physiopathology / Q000628:therapy; D000818:Animals; D057005:Bioengineering / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009474:Neurons / Q000502:physiology; D010513:Periodontal Ligament / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000294:innervation / Q000502:physiology* / Q000503:physiopathology / Q000637:transplantation