title: Gene expression profiling in laser-microdissected bone marrow megakaryocytes.
abstract: Gene expression analysis of the megakaryocytic lineage requires isolation of megakaryocytes from their bone marrow microenvironment. Laser microdissection of megakaryocytes from diagnostic bone marrow biopsies allows analysis of standardised formalin-fixed samples that reflect the in situ grown status quo of a physiological or pathological condition. Taking into account that in neoplastic proliferation, e.g. myeloproliferative neoplasms, non-neoplastic haematopoietic clones proliferate in parallel, this morphology-based isolation enables selective analysis of the aberrant megakaryocytic population. Two different laser microdissection devices are presented, and the details of RNA extraction and subsequent real-time qPCR gene expression analysis of mRNA and microRNA are provided.
authors.affiliation: Institute of Pathology, Hannover Medical School, Hannover, Germany. Hussein.Kais@MH-Hannover.de
authors.forename: Kais
authors.initials: K
authors.lastname: Hussein
mesh_terms: D018775:Bone Demineralization Technique / Q000379:methods; D001853:Bone Marrow; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D042282:Microdissection / Q000379:methods*; D008867:Microtomy / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016707:Tissue Fixation / Q000379:methods
chemical_list: D012313:RNA