title: Laser capture microdissection: methods and applications.
abstract: Laser microdissection is a nonmolecular, minimally disruptive method to obtain cytologically and/or phenotypically defined cells or groups of cells from heterogeneous tissues. It is a versatile technology and allows the preparation of homogenous isolates of specific subpopulations of cells from which RNA/DNA or protein can be extracted for RT-polymerase chain reaction (PCR), quantitative PCR, Western blot analyses, and mass spectrophotometry.
authors.affiliation: Boston University School of Medicine, Boston, MA, USA.
authors.forename: Kristen
authors.initials: K
authors.lastname: Decarlo
authors.forename: Andrew
authors.initials: A
authors.lastname: Emley
authors.forename: Ophelia E
authors.initials: OE
authors.lastname: Dadzie
authors.forename: Meera
authors.initials: M
authors.lastname: Mahalingam
mesh_terms: D002469:Cell Separation / Q000379:methods; D003141:Communicable Diseases / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D009696:Nucleic Acids / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D020543:Proteome / Q000378:metabolism; D017421:Sequence Analysis / Q000379:methods; D059010:Single-Cell Analysis; D016707:Tissue Fixation / Q000379:methods
chemical_list: D009696:Nucleic Acids; D011506:Proteins; D020543:Proteome