title: Post-chemiluminescence determination of chloramphenicol based on luminol-potassium periodate system.
abstract: A post-chemiluminescence (PCL) phenomenon was observed when chloramphenicol was injected into a mixture of luminol and potassium periodate after the chemiluminescence (CL) reaction of luminol-potassium periodate had finished. The possible reaction mechanism was proposed based on studies of the CL kinetic characteristics, the CL spectra, the fluorescence spectra and the UV-vis absorption spectra of the related substances. Based on the PCL reaction, a rapid and sensitive method for the determination of chloramphenicol was established. The linear response range was 6.0 × 10(-7) -1.0 × 10(-5) mol/L, with a correlation coefficient of 0.9986. The relative standard deviation (RSD) for 5.0 × 10(-6) mol/L chloramphenicol was 2.3% (n = 11). The detection limit was 1.6 × 10(-7) mol/L. The method has been applied to the determination of chloramphenicol in pharmaceutical samples with satisfactory results.
authors.affiliation: Key Laboratory of Luminescence and Real-time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Yang
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
mesh_terms: D002701:Chloramphenicol / Q000032:analysis*; D049449:Luminescence; D008165:Luminol / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*
chemical_list: D017680:Potassium Compounds; D010504:Periodic Acid; D008165:Luminol; D002701:Chloramphenicol; C037192:potassium periodate