title: DNA/NYVAC vaccine regimen induces HIV-specific CD4 and CD8 T-cell responses in intestinal mucosa.
abstract: In the present study, we have investigated the anatomic distribution in blood and gut mucosal tissues of memory poxvirus-specific CD4 and CD8 T cells in subjects vaccinated with smallpox and compared it with vector (NYVAC)-specific and HIV insert-specific T-cell responses induced by an experimental DNA-C/ NYVAC-C vaccine regimen. Smallpox-specific CD4 T-cell responses were present in the blood of 52% of the subjects studied, while smallpox-specific CD8 T cells were rarely detected (12%). With one exception, smallpox-specific T cells were not measurable in gut tissues. Interestingly, NYVAC vector-specific and HIV-specific CD4 and CD8 T-cell responses were detected in almost 100% of the subjects immunized with DNA-C/NYVAC-C in blood and gut tissues. The large majority (83%) of NYVAC-specific CD4 T cells expressed α4β7 integrins and the HIV coreceptor CCR5. These results demonstrate that the experimental DNA-C/NYVAC-C HIV vaccine regimen induces the homing of potentially protective HIV-specific CD4 and CD8 T cells in the gut, the port of entry of HIV and one of the major sites for HIV spreading and the depletion of CD4 T cells.
authors.affiliation: Department of Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne, Switzerland. Matthieu.Perreau@chuv.ch
authors.forename: Matthieu
authors.initials: M
authors.lastname: Perreau
authors.forename: Hugh C
authors.initials: HC
authors.lastname: Welles
authors.forename: Alexandre
authors.initials: A
authors.lastname: Harari
authors.forename: Olivia
authors.initials: O
authors.lastname: Hall
authors.forename: Ricardo
authors.initials: R
authors.lastname: Martin
authors.forename: Michel
authors.initials: M
authors.lastname: Maillard
authors.forename: Gian
authors.initials: G
authors.lastname: Dorta
authors.forename: Pierre-Alexandre
authors.initials: PA
authors.lastname: Bart
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Kremer
authors.forename: Jim
authors.initials: J
authors.lastname: Tartaglia
authors.forename: Ralf
authors.initials: R
authors.lastname: Wagner
authors.forename: Mariano
authors.initials: M
authors.lastname: Esteban
authors.forename: Yves
authors.initials: Y
authors.lastname: Levy
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pantaleo
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology*; D000328:Adult; D001769:Blood / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D008875:Middle Aged; D012900:Smallpox Vaccine / Q000008:administration & dosage / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; C078787:NYVAC vaccine; D012900:Smallpox Vaccine; D019444:Vaccines, DNA; D014765:Viral Vaccines