title: High prevalence of multiple human papillomavirus infection in Japanese patients with invasive uterine cervical cancer.
abstract: OBJECTIVE
  Multiple human papillomavirus (HPV) infection of the uterine cervix has been suggested as a risk factor for persistent HPV infection, resulting in the development of invasive cervical cancer. The aim of this study was to reveal the actual state of multiple HPV infection in Japanese patients with invasive cervical cancer.
  
  
  METHODS
  Sixty fresh-frozen invasive cervical cancer tissues were examined for genotyping of HPV. The presence of HPV genotypes was determined with an HPV-DNA array, which can discriminate 25 different HPV genotypes with high sensitivity and specificity.
  
  
  RESULTS
  Among 60 samples, 59 (96.7%) were positive for HPV. The three common genotypes were HPV-16 (83.3%), HPV-18 (45.0%) and HPV-52 (28.3%). Multiple HPV infection was observed in 47 of 60 samples (78.3%), among which 42 were infected with more than one high-risk genotype (70.0%). Multiple high-risk HPV infection was significantly more prevalent in patients below 40 years old (14/15, 93.3%) than in patients 40 years of age and over (28/45, 62.2%).
  
  
  CONCLUSIONS
  The HPV-DNA array is the preferred method to detect HPV genotypes. Multiple HPV infection in Japanese patients with invasive cervical cancer seemed to be more frequent than reported in the literature.
authors.affiliation: Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Hidemichi
authors.initials: H
authors.lastname: Watari
authors.forename: Rie
authors.initials: R
authors.lastname: Michimata
authors.forename: Motoaki
authors.initials: M
authors.lastname: Yasuda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ishizu
authors.forename: Utano
authors.initials: U
authors.lastname: Tomaru
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Mohamed K
authors.initials: MK
authors.lastname: Hassan
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sakuragi
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000209:etiology / Q000821:virology; D000328:Adult; D000368:Aged; D018196:Carcinoma, Adenosquamous / Q000150:complications / Q000209:etiology / Q000821:virology; D018288:Carcinoma, Small Cell / Q000150:complications / Q000209:etiology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000209:etiology / Q000821:virology; D060085:Coinfection / Q000150:complications / Q000453:epidemiology / Q000821:virology; D015344:DNA Probes, HPV; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D030361:Papillomavirus Infections / Q000150:complications* / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000150:complications* / Q000209:etiology / Q000821:virology; D055815:Young Adult
chemical_list: D015344:DNA Probes, HPV