title: Mimotopes of mutalysin-II from Lachesis muta snake venom induce hemorrhage inhibitory antibodies upon vaccination of rabbits.
abstract: Mutalysin-II (mut-II) from Lachesis muta snake venom is an endopeptidase with hemorrhagic activity. A mAb against mutalysin-II that neutralized the hemorrhagic effect was produced previously. To identify the mAb epitopes, sets of 15-mer overlapping peptides covering the mut-II amino acid sequence were synthesized using the SPOT method and tested but failed to react with the mAb. Using a phage-display approach seventeen clones reactive with mAb were identified. Additional immunoassays with the peptides and mAb identified the QCTMDQGRLRCR, TCATDQGRLRCT, HCFHDQGRVRCA, HCTMDQGRLRCR and SCMLDQGRSRCR sequences as possible epitopes. Immunization of rabbits with these peptides induced antibodies that recognize mut-II and protected against the hemorrhagic effects of Lachesis venom.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte-MG, Brazil.
authors.forename: R A
authors.initials: RA
authors.lastname: Machado de Avila
authors.forename: S
authors.initials: S
authors.lastname: Stransky
authors.forename: M
authors.initials: M
authors.lastname: Velloso
authors.forename: P
authors.initials: P
authors.lastname: Castanheira
authors.forename: F S
authors.initials: FS
authors.lastname: Schneider
authors.forename: E
authors.initials: E
authors.lastname: Kalapothakis
authors.forename: E F
authors.initials: EF
authors.lastname: Sanchez
authors.forename: C
authors.initials: C
authors.lastname: Nguyen
authors.forename: F
authors.initials: F
authors.lastname: Molina
authors.forename: C
authors.initials: C
authors.lastname: Granier
authors.forename: C
authors.initials: C
authors.lastname: Chávez-Olórtegui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D003435:Crotalid Venoms / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006470:Hemorrhage / Q000276:immunology / Q000517:prevention & control*; D008666:Metalloendopeptidases / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D011817:Rabbits; D014611:Vaccination
chemical_list: D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D000939:Epitopes; C072644:Lachesis venom; D019151:Peptide Library; D010455:Peptides; D008666:Metalloendopeptidases; C068795:hemorrhagic factor LHFII