title: Using superfolder green fluorescent protein for periplasmic protein localization studies.
abstract: Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, Armenise Building, Room 302A, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: Thuy
authors.initials: T
authors.lastname: Dinh
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Bernhardt
mesh_terms: D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D019897:Periplasm / Q000737:chemistry*; D033903:Periplasmic Proteins / Q000032:analysis* / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics; D013194:Staining and Labeling / Q000379:methods
chemical_list: D033903:Periplasmic Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins