title: [Absorption of flavonoids from Abelmoschus manihot extract by in situ intestinal perfusion].
abstract: To explore the mechanism of the absorption of flavonoids from Abelmoschus manihot flowers, in situ intestinal recirculation was performed to study the effect of the absorption at different concentrations and different intestinal regions. To evaluate the conditions of the absorption of six flavonoids from Abelmoschus manihot flowers, the concentrations of Abelmoschus manihot in the perfusion solution were determined by HPLC at predesigned time. And we have investigated the inhibitory effect of six flavonoids from Abelmoschus manihot flowers on P-glycoprotein (P-gp) drug efflux pump. The results demonstrated that the absorption rates of flavonoids from Abelmoschus manihot flowers are not significantly different (P > 0.05) at various drug concentrations, the absorption of flavonoids from Abelmoschus manihot flowers is a first-order process with the passive diffusion mechanism. The absorption rates of each of flavonoids are significantly different. The absorption rate of flavonoid glycoside was lower than that of aglycone; the flavonoids from Abelmoschus manihot flowers could be absorbed in all of the intestinal segments. The best parts of intestine to absorb hyperoside and myricetin are jejunum and duodenum, separately. Verapamil could enhance the absorption of isoquercitrin, hyperoside, myricetin and quercetin-3'-O-glucoside by inhibiting P-glycoprotein (P-gp) drug efflux pump.
authors.affiliation: Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
authors.forename: Cai-fu
authors.initials: CF
authors.lastname: Xue
authors.forename: Jian-ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Yan
authors.initials: Y
authors.lastname: Shu
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000037:antagonists & inhibitors; D028483:Abelmoschus / Q000737:chemistry*; D000818:Animals; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification / Q000493:pharmacokinetics*; D035264:Flowers / Q000737:chemistry; D005960:Glucosides; D007408:Intestinal Absorption / Q000187:drug effects*; D008297:Male; D010477:Perfusion; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D014700:Verapamil / Q000494:pharmacology
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; C532085:quercetin 3'-O-glucoside; C016527:isoquercitrin; C087346:hibifolin; C040015:myricetin; C021304:hyperoside; D011794:Quercetin; D014700:Verapamil