title: Detection of bioconjugated quantum dots passivated with different ligands for bio-applications.
abstract: Bioconjugation of quantum dots has resulted in a significant increase in resolution of biological fluorescent labeling. This intrinsic property of quantum dots can be utilized for sensitive detection of target analytes with high sensitivity; including pathogenic bacteria and cancer monitoring. The quantum dots and quantum dot doped silica nanoparticles exhibit prominent emission peaks when excited at 400 nm but on conjugation to model rabbit antigoat antibodies exhibit diminished intensity of emission peak at 600 nm. It shows that photoluminescence intensity of conjugated quantum dots and quantum dot doped silica nanoparticles could permit the detection of bioconjugation. Samples of conjugated and unconjugated quantum dots and quantum dot doped silica nanoparticles were subjected to enzyme linked immunosorbent assay for further confirmation of bioconjugation. In the present study ligand exchange, bioconjugation, fluorescence detection of bioconjugated quantum dots and quantum dot doped silica nanoparticles and further confirmation of bioconjugation by enzyme linked immunosorbent assay has been described.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New Delhi 110016, India.
authors.forename: Gurpal
authors.initials: G
authors.lastname: Singh
authors.forename: Neelam Hazoor
authors.initials: NH
authors.lastname: Zaidi
authors.forename: Udit
authors.initials: U
authors.lastname: Soni
authors.forename: Manoj
authors.initials: M
authors.lastname: Gautam
authors.forename: Richa
authors.initials: R
authors.lastname: Jackeray
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
authors.forename: Sameer
authors.initials: S
authors.lastname: Sapra
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D008024:Ligands; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D045663:Quantum Dots; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D008024:Ligands; D012822:Silicon Dioxide