title: Stability of lyophilized human platelets loaded with small molecule carbohydrates.
abstract: Long-term preservation of platelets is a great challenge for blood transfusion centers, due to the required narrow storage temperature arange (22 ± 2 degree C). Short shelf life and potential bacterial growth often lead to the shortage of high-quality platelets. Freeze-dried preservation is thus believed to be a potential solution for long-term platelet storage without losing the hemostasis function. Here we report a new platelet preservation method, which uses small molecule carbohydrates to extend storage time and to maintain platelet function. The activities of lyophilized platelets that were stabilized with small molecule carbohydrate (e.g., cell viability, mean platelet volume, activation characteristics, and aggregation kinetics) were maintained after storage of 30, 60, and 90 days at room temperature, 4 degree C, and -20 degree C. The recovery of freeze-dried platelets was 87 percent in comparison to fresh platelets. The mean platelet volume of rehydrated platelets increased (from 6.8 fl to 8.0 fl). About 40 percent of rehydrated platelets was in the early-activated stage (PCA-1 positive) and 30 percent was in the terminal-activated stage (CD62P positive). The cell viability was about 60 percent as measured with CMFDA vital probes. The aggregation rate of rehydrated platelets after 90-day storage was similar to fresh platelets stored at 22 degree C ± 2 degree C.
authors.affiliation: Beijing Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Wang
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.forename: W
authors.initials: W
authors.lastname: Wan
authors.forename: M X
authors.initials: MX
authors.lastname: Liu
authors.forename: S P
authors.initials: SP
authors.lastname: Ren
authors.forename: G B
authors.initials: GB
authors.lastname: Quan
authors.forename: Y
authors.initials: Y
authors.lastname: Han
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002241:Carbohydrates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D005612:Freeze Drying / Q000379:methods*; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D054852:Small Molecule Libraries / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D002241:Carbohydrates; D019007:P-Selectin; D054852:Small Molecule Libraries