title: A NMR reverse diffusion filter for the simplification of spectra of complex mixtures and the study of drug receptor interactions.
abstract: A reverse diffusion filter NMR experiment (Drev) is proposed for the study of small molecules in binding with macromolecules. The filtering efficiency of Drev to eliminate the signals of the macromolecule is shown to be superior to conventional transverse relaxation filters at least for macromolecules containing a significant fraction of flexible residues. The Drev filter was also a useful complement for ligand-based NMR screening in combination with saturation transfer difference experiments.
authors.affiliation: Unidade de Resonancia Magnética, Edif. CACTUS, RIAIDT, Campus Vida, University of Santiago de Compostela, A Coruña 15706, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Vega-Vázquez
authors.forename: J C
authors.initials: JC
authors.lastname: Cobas
authors.forename: F F
authors.initials: FF
authors.lastname: Oliveira de Sousa
authors.forename: M
authors.initials: M
authors.lastname: Martin-Pastor
mesh_terms: D045424:Complex Mixtures / Q000737:chemistry; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry*; D004058:Diffusion; D007213:Indomethacin / Q000737:chemistry*; D007700:Kinetics; D008024:Ligands; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D011955:Receptors, Drug / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry; D015028:Zein / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D016861:Cyclooxygenase Inhibitors; D008024:Ligands; D011955:Receptors, Drug; D015028:Zein; D007213:Indomethacin