title: Complete plastic nanofluidic devices for DNA analysis via direct imprinting with polymer stamps.
abstract: Development of all polymer-based nanofluidic devices using replication technologies, which is a prerequisite for providing devices for a larger user base, is hampered by undesired substrate deformation associated with the replication of multi-scale structures. Therefore, most nanofluidic devices have been fabricated in glass-like substrates or in a polymer resist layer coated on a substrate. This letter presents a rapid, high fidelity direct imprinting process to build polymer nanofluidic devices in a single step. Undesired substrate deformation during imprinting was significantly reduced through the use of a polymer stamp made from a UV-curable resin. The integrity of the enclosed all polymer-based nanofluidic system was verified by a fluorescein filling experiment and translocation/stretching of λ-DNA molecules through the nanochannels. It was also found that the funnel-like design of the nanochannel inlet significantly improved the entrance of DNA molecules into nanochannels compared to an abrupt nanochannel/microfluidic network interface.
authors.affiliation: Mechanical Engineering Department, Louisiana State University, Louisiana, USA.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Wu
authors.forename: Rattikan
authors.initials: R
authors.lastname: Chantiwas
authors.forename: Alborz
authors.initials: A
authors.lastname: Amirsadeghi
authors.forename: Steven A
authors.initials: SA
authors.lastname: Soper
authors.forename: Sunggook
authors.initials: S
authors.lastname: Park
mesh_terms: D004247:DNA / Q000032:analysis*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D054802:Molecular Imprinting; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers; D004247:DNA; D016650:Fluorescein-5-isothiocyanate