title: Nitric oxide synthesis and biological functions of nitric oxide released from ruthenium compounds.
abstract: During three decades, an enormous number of studies have demonstrated the critical role of nitric oxide (NO) as a second messenger engaged in the activation of many systems including vascular smooth muscle relaxation. The underlying cellular mechanisms involved in vasodilatation are essentially due to soluble guanylyl-cyclase (sGC) modulation in the cytoplasm of vascular smooth cells. sGC activation culminates in cyclic GMP (cGMP) production, which in turn leads to protein kinase G (PKG) activation. NO binds to the sGC heme moiety, thereby activating this enzyme. Activation of the NO-sGC-cGMP-PKG pathway entails Ca(2+) signaling reduction and vasodilatation. Endothelium dysfunction leads to decreased production or bioavailability of endogenous NO that could contribute to vascular diseases. Nitrosyl ruthenium complexes have been studied as a new class of NO donors with potential therapeutic use in order to supply the NO deficiency. In this context, this article shall provide a brief review of the effects exerted by the NO that is enzymatically produced via endothelial NO-synthase (eNOS) activation and by the NO released from NO donor compounds in the vascular smooth muscle cells on both conduit and resistance arteries, as well as veins. In addition, the involvement of the nitrite molecule as an endogenous NO reservoir engaged in vasodilatation will be described.
authors.affiliation: Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brasil.
authors.forename: A C
authors.initials: AC
authors.lastname: Pereira
authors.forename: M
authors.initials: M
authors.lastname: Paulo
authors.forename: A V
authors.initials: AV
authors.lastname: Araújo
authors.forename: G J
authors.initials: GJ
authors.lastname: Rodrigues
authors.forename: L M
authors.initials: LM
authors.lastname: Bendhack
mesh_terms: D000818:Animals; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000503:physiopathology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D051381:Rats; D017975:Ruthenium Compounds / Q000378:metabolism*; D014664:Vasodilation / Q000502:physiology
chemical_list: D020030:Nitric Oxide Donors; D017975:Ruthenium Compounds; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III