title: Neurobiological activity of Parawixin 10, a novel anticonvulsant compound isolated from Parawixia bistriata spider venom (Araneidae: Araneae).
abstract: The neurobiological activity of Parawixin 10, isolated from Parawixia bistriata spider venom, was investigated. Cannulas were implanted in the lateral ventricles of Wistar rats (200-250 g, n=6-8 per group) to perform anticonvulsant and behavioral assays, and synaptosomes from cerebral cortices of male Wistar rats were used for neurochemical studies. The results indicate that pretreatment with Parawixin 10 prevents the onset of seizures induced with kainic acid, N-methyl-D-aspartate, and pentylenetetrazole in a dose-response manner. Lower doses of Parawixin 10 significantly increased the latency to onset of kainic acid-, pentylenetetrazole-, and N-methyl-D-aspartate-induced seizures. There were maximum increases of 79% in L-[(3)H]glutamine uptake and 40% in [(3)H]glycine uptake; [(3)H]GABA uptake did not change. The findings demonstrate that this novel compound from P. bistriata venom exerts a pharmacological effect on the glutamatergic and glycinergic systems.
authors.affiliation: Neurobiology and Venoms Laboratory, Department of Biology, FFCLRP, University of São Paulo, São Paulo, Brazil.
authors.forename: Helene Aparecida
authors.initials: HA
authors.lastname: Fachim
authors.forename: Alexandra Olimpio Siqueira
authors.initials: AO
authors.lastname: Cunha
authors.forename: Adriana Colsera
authors.initials: AC
authors.lastname: Pereira
authors.forename: René Oliveira
authors.initials: RO
authors.lastname: Beleboni
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gobbo-Neto
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
authors.forename: Joaquim
authors.initials: J
authors.lastname: Coutinho-Netto
authors.forename: Wagner Ferreira
authors.initials: WF
authors.lastname: dos Santos
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry* / Q000627:therapeutic use*; D001259:Ataxia / Q000188:drug therapy / Q000209:etiology; D002540:Cerebral Cortex / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D018698:Glutamic Acid / Q000378:metabolism; D005998:Glycine / Q000187:drug effects; D007608:Kainic Acid / Q000633:toxicity; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D016202:N-Methylaspartate / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D013111:Spider Venoms / Q000737:chemistry* / Q000627:therapeutic use*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D014316:Tritium / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D000927:Anticonvulsants; D018690:Excitatory Amino Acid Agonists; D013111:Spider Venoms; C569863:parawixin 10, Parawixia bistriata; D014316:Tritium; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D007608:Kainic Acid; D005998:Glycine