title: Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms.
abstract: We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.
authors.affiliation: Department of Life Science and Medical Bioscience, Waseda University, Shinjuku-ku, Japan.
authors.forename: Soji
authors.initials: S
authors.lastname: Morishita
authors.forename: Norio
authors.initials: N
authors.lastname: Komatsu
authors.forename: Keita
authors.initials: K
authors.lastname: Kirito
authors.forename: Aya H
authors.initials: AH
authors.lastname: Koda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sekiguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Naohiro
authors.initials: N
authors.lastname: Noda
mesh_terms: D000483:Alleles; D019943:Amino Acid Substitution / Q000235:genetics; D001667:Binding, Competitive; D019046:Bone Marrow Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D003362:Cost-Benefit Analysis; D004252:DNA Mutational Analysis / Q000379:methods / Q000592:standards; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D018628:Gene Dosage; D006801:Humans; D053614:Janus Kinase 2 / Q000032:analysis / Q000235:genetics*; D008954:Models, Biological; D009196:Myeloproliferative Disorders / Q000235:genetics; D010649:Phenylalanine / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000191:economics* / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014633:Valine / Q000235:genetics
chemical_list: D015342:DNA Probes; D010649:Phenylalanine; C507924:JAK2 protein, human; D053614:Janus Kinase 2; D014633:Valine