title: Complement activation products in liquid stored plasma and C3a kinetics after transfusion of autologous plasma.
abstract: OBJECTIVE
  Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg).
  
  
  METHODS
  Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg), C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors.
  
  
  RESULTS
  Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg), C3d,g and sC5b-9 from day 0 and low iC3, than plasma prepared by other methods. By storage day 7, C3a-(desArg)-levels were above the reference value in 88% of all components. After re-infusion of autologous plasma with high C3a-(desArg) content, there were rapid a(1) and a(2)-distribution followed by a slower b-elimination phase.
  
  
  CONCLUSIONS
  Plasma components prepared by different methods and stored in the liquid phase differ significantly in the amount and timing of complement activation. C3a-(desArg) present in plasma is rapidly eliminated after transfusion. Autologous plasma could be used to study complement kinetics in different clinical situations.
authors.affiliation: Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden. rut.norda@akademiska.se
authors.forename: R
authors.initials: R
authors.lastname: Norda
authors.forename: U
authors.initials: U
authors.lastname: Schött
authors.forename: O
authors.initials: O
authors.lastname: Berséus
authors.forename: O
authors.initials: O
authors.lastname: Akerblom
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
authors.forename: K N
authors.initials: KN
authors.lastname: Ekdahl
authors.forename: B G
authors.initials: BG
authors.lastname: Stegmayr
authors.forename: F
authors.initials: F
authors.lastname: Knutson
mesh_terms: D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion / Q000379:methods*; D003167:Complement Activation / Q000276:immunology*; D015926:Complement C3a / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma / Q000276:immunology*
chemical_list: C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a