title: Fgf is required to regulate anterior-posterior patterning in the Xenopus lateral plate mesoderm.
abstract: Given that the lateral plate mesoderm (LPM) gives rise to the cardiovascular system, identifying the cascade of signalling events that subdivides the LPM into distinct regions during development is an important question. Retinoic acid (RA) is known to be necessary for establishing the expression boundaries of important transcription factors that demarcate distinct regions along the anterior posterior axis of the LPM. Here, we demonstrate that fibroblast growth factor (Fgf) signalling is also necessary for regulating the expression domains of the same transcription factors (nkx2.5, foxf1, hand1 and sall3) by restricting the RA responsive LPM domains. When Fgf signalling is inhibited in neurula stage embryos, the more posterior LPM expression domains are lost, while the more anterior domains are extended further posterior. The domain changes are maintained throughout development as Fgf inhibition results in similar domain changes in late stage embryos. We also demonstrate that Fgf signalling is necessary for both the initiation of heart specification, and for maintaining heart specification until overt differentiation occurs. Fgf signalling is also necessary to restrict vascular patterning and create a vascular free domain in the posterior end of the LPM that correlates with the expression of hand1. Finally, we show cross talk between the RA and Fgf signalling pathways in the patterning of the LPM. We suggest that this tissue wide patterning event, active during the neurula stage, is an initial step in regional specification of the LPM, and this process is an essential early event in LPM patterning.
authors.affiliation: Children's Health Research Institute, 800 Commissioners Road E., London, Ontario, Canada.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Deimling
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Drysdale
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D019521:Body Patterning; D002319:Cardiovascular System / Q000196:embryology / Q000254:growth & development; D005260:Female; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology* / Q000254:growth & development*; D017403:In Situ Hybridization; D008648:Mesoderm / Q000196:embryology / Q000254:growth & development*; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D014212:Tretinoin / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005346:Fibroblast Growth Factors; D051858:Forkhead Transcription Factors; D014157:Transcription Factors; D014212:Tretinoin; D029867:Xenopus Proteins; C411856:FoxF1 protein, Xenopus; C097341:helix-loop-helix protein, eHAND