title: Rapid speciation and quantification of selenium compounds by HPLC-ICP MS using multiple standards labelled with different isotopes.
abstract: Speciation analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP MS) is now commonly used to investigate metabolic and toxicological aspects of some metals and metalloids. We have developed a rapid method for simultaneous identification and quantification of metabolites of selenium (Se) compounds using multiple standards labelled with different isotopes. A mixture of the labelled standards was spiked in a selenised garlic extract and the sample was subjected to speciation analysis by HPLC-ICP MS. The selenised garlic contains γ-glutamyl-methylselenocysteine, methylselenocysteine, and selenomethionine and the concentrations of those Se compounds were 723.8, 414.8, and 310.7 ng Se ml(-1), respectively. The isotopically labelled standards were also applied to the speciation of Se in rat urine. Selenate, methylselenonic acid, selenosugar, and trimethyselenium ions were found to be excreted by the present speciation procedure. Multiple standards labelled with different stable isotopes enable high-throughput identification and quantitative measurements of Se metabolites.
authors.affiliation: Environmental Nanotoxicology Section, Research Center for Environmental Risk, National Institute for Environmental Studies, Ibaraki, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ohta
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Seishiro
authors.initials: S
authors.lastname: Hirano
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium / Q000032:analysis / Q000378:metabolism / Q000652:urine; D005737:Garlic / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D007201:Indicator Dilution Techniques / Q000295:instrumentation / Q000592:standards; D007553:Isotope Labeling / Q000379:methods* / Q000592:standards; D007554:Isotopes / Q000032:analysis / Q000378:metabolism / Q000652:urine; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000032:analysis / Q000378:metabolism / Q000652:urine; D018036:Selenium Compounds / Q000032:analysis* / Q000378:metabolism / Q000652:urine; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D007554:Isotopes; D018036:Selenium Compounds; D003903:Deuterium; D012643:Selenium