title: Recombinant canine parvovirus-like particles express foreign epitopes in silkworm pupae.
abstract: The capsid structural protein VP2 of canine parvovirus (CPV) can self-assemble into highly organized virus-like particles (VLPs) and retain major immunoreactivity. In this study, different recombinant baculoviruses that expressed varying fusion proteins of the CPV VP2 protein with the T cell determinant and/or the linear virus-neutralizing epitope of rabies virus (RV) were generated. Infection with these baculoviruses changed BmN cell morphology and inhibited their proliferation as well as damaged silkworms and pupae. However, infection with these baculoviruses induced high levels of recombinant protein expression in silkworms and pupae. More importantly, these fusion proteins self-assembled VLPs with properties similar to CPV virions and retained their VP2-specific immunoreactivity, but some retained their RV-specific immunoreactivity. Interestingly, only one fusion protein, T-VP2, maintained its haemagglutination activity. These data indicated that these insertions and replacements in the loop 2 of VP2 did not interfere with the formation of VLP, and silkworms and pupae could act as a low-costing bioreactor for the production of heterologous proteins. Therefore, our findings may provide a new framework for the development of subunit vaccines against RV and CPV.
authors.affiliation: Agricultural Division, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Feng
authors.forename: Meng
authors.initials: M
authors.lastname: Liang
authors.forename: Hua-lei
authors.initials: HL
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Ping-sen
authors.initials: PS
authors.lastname: Zhao
authors.forename: Xing-jia
authors.initials: XJ
authors.lastname: Shen
authors.forename: Ren-zhou
authors.initials: RZ
authors.lastname: Zhang
authors.forename: Gui-qiu
authors.initials: GQ
authors.lastname: Hu
authors.forename: Yu-qei
authors.initials: YQ
authors.lastname: Gao
authors.forename: Cheng-yu
authors.initials: CY
authors.lastname: Wang
authors.forename: Tie-cheng
authors.initials: TC
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Song-tao
authors.initials: ST
authors.lastname: Yang
authors.forename: Xian-zhu
authors.initials: XZ
authors.lastname: Xia
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000276:immunology; D012831:Bombyx / Q000276:immunology* / Q000378:metabolism; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000276:immunology; D002478:Cells, Cultured; D004285:Dogs; D000939:Epitopes / Q000096:biosynthesis* / Q000276:immunology; D005822:Genetic Vectors; D006386:Hemagglutination Tests; D017993:Parvovirus, Canine / Q000276:immunology*; D011679:Pupa / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology
chemical_list: D036022:Capsid Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins