title: A re-investigation of [Fe(L-cysteinate)2(CO)2]2-: an example of non-heme CO coordination of possible relevance to CO binding to ion channel receptors.
abstract: [Fe(L-cysteinate)(2)(CO)(2)](2-) is a CO releasing molecule which has low cytotoxicity to RAW264.7 macrophages. It provides an example of CO binding using ligands available to ion channels which use CO as a signalling molecule in the absence of heme. Previous work has shown that this compound consists of five isomers and it was proposed that the two isomers with trans-dicarbonyls are dominant. In this work the isomers are re-assigned and shown to be capable of releasing CO, albeit too slowly to act as a signalling receptor. It is shown that by linking the two L-cysteines together to form [Fe(SCH(2)CH{CO(2)H}NHCH(2))(2)(CO)(2)], only one isomer is isolated.
authors.affiliation: Department of Chemistry, University of Sheffield, Sheffield, United Kingdom, S3 7HF.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Hewison
authors.forename: Tony R
authors.initials: TR
authors.lastname: Johnson
authors.forename: Brian E
authors.initials: BE
authors.lastname: Mann
authors.forename: Anthony J H M
authors.initials: AJ
authors.lastname: Meijer
authors.forename: Philip
authors.initials: P
authors.lastname: Sawle
authors.forename: Roberto
authors.initials: R
authors.lastname: Motterlini
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005296:Ferrous Compounds / Q000737:chemistry* / Q000633:toxicity; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D011485:Protein Binding
chemical_list: D005296:Ferrous Compounds; D051036:Large-Conductance Calcium-Activated Potassium Channels; D002248:Carbon Monoxide; D003545:Cysteine