title: Biomarkers of oxidative stress and endothelial dysfunction after tourniquet release in children.
abstract: Pneumatic tourniquets are widely used in pediatric extremity surgery to provide a bloodless field and facilitate dissection. This prospective study was carried out to examine possible effect of different anesthesia techniques on oxidative stress and endothelial dysfunction connected with ischemia-reperfusion injury during extremity operations at children's age. Patients were randomized into three groups of 15 patients each: general inhalational anesthesia with sevoflurane (group S), total intravenous anesthesia with propofol (group T) and regional anesthesia (group R). Venous blood samples for determination of the malondialdehyde in plasma and erythrocytes, protein carbonyl groups concentration as well as plasma nitrites and nitrates level and xanthine oxidase activity were obtained at four time points: before peripheral nerve block and induction of general anesthesia (baseline), 1 min before tourniquet release, 5 and 20 min after tourniquet release. This study demonstrates that total intravenous anesthesia with propofol and regional anesthesia techniques provide better antioxidant defense and reduce endothelial dysfunction than general inhalational anesthesia with sevoflurane during tourniquet application in pediatric extremity surgery.
authors.affiliation: Centre for Anesthesiology and Reanimatology, Clinical Centre Nis, Medical Faculty, University of Nis, Nis, Serbia. ibudic@open.telekom.rs
authors.forename: I
authors.initials: I
authors.lastname: Budic
authors.forename: D
authors.initials: D
authors.lastname: Pavlovic
authors.forename: G
authors.initials: G
authors.lastname: Kocic
authors.forename: T
authors.initials: T
authors.lastname: Cvetkovic
authors.forename: D
authors.initials: D
authors.lastname: Simic
authors.forename: J
authors.initials: J
authors.lastname: Basic
authors.forename: D
authors.initials: D
authors.lastname: Zivanovic
mesh_terms: D000293:Adolescent; D000704:Analysis of Variance; D000769:Anesthesia, Inhalation; D000771:Anesthesia, Intravenous; D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D000779:Anesthetics, Local; D015415:Biomarkers / Q000097:blood; D002045:Bupivacaine; D002648:Child; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008738:Methyl Ethers; D009407:Nerve Block; D009566:Nitrates / Q000097:blood; D009573:Nitrites / Q000097:blood; D019637:Orthopedic Procedures; D018384:Oxidative Stress; D015742:Propofol; D011446:Prospective Studies; D050050:Protein Carbonylation; D055771:Serbia; D000077149:Sevoflurane; D013997:Time Factors; D014111:Tourniquets / Q000009:adverse effects; D014969:Xanthine Oxidase / Q000097:blood
chemical_list: D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D000779:Anesthetics, Local; D015415:Biomarkers; D008738:Methyl Ethers; D009566:Nitrates; D009573:Nitrites; D000077149:Sevoflurane; D008315:Malondialdehyde; D014969:Xanthine Oxidase; D002045:Bupivacaine; D015742:Propofol