title: Role of pagL and lpxO in Bordetella bronchiseptica lipid A biosynthesis.
abstract: PagL and LpxO are enzymes that modify lipid A. PagL is a 3-O deacylase that removes the primary acyl chain from the 3 position, and LpxO is an oxygenase that 2-hydroxylates specific acyl chains in the lipid A. pagL and lpxO homologues have been identified in the genome of Bordetella bronchiseptica, but in the current structure for B. bronchiseptica lipid A the 3 position is acylated and 2-OH acylation is not reported. We have investigated the role of B. bronchiseptica pagL and lpxO in lipid A biosynthesis. We report a different structure for wild-type (WT) B. bronchiseptica lipid A, including the presence of 2-OH-myristate, the presence of which is dependent on lpxO. We also demonstrate that the 3 position is not acylated in the major WT lipid A structures but that mutation of pagL results in the presence of 3-OH-decanoic acid at this position, suggesting that lipid A containing this acylation is synthesized but that PagL removes most of it from the mature lipid A. These data refine the structure of B. bronchiseptica lipid A and demonstrate that pagL and lpxO are involved in its biosynthesis.
authors.affiliation: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: I
authors.initials: I
authors.lastname: MacArthur
authors.forename: J W
authors.initials: JW
authors.lastname: Jones
authors.forename: D R
authors.initials: DR
authors.lastname: Goodlett
authors.forename: R K
authors.initials: RK
authors.lastname: Ernst
authors.forename: A
authors.initials: A
authors.lastname: Preston
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016950:Bordetella bronchiseptica / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D007850:Lauric Acids / Q000032:analysis; D008050:Lipid A / Q000096:biosynthesis* / Q000737:chemistry*; D009226:Myristates / Q000032:analysis; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007850:Lauric Acids; D008050:Lipid A; D009226:Myristates; C022145:3-hydroxydodecanoic acid; D010105:Oxygenases; D002265:Carboxylic Ester Hydrolases