title: Mosquito larvicidal and ovicidal properties of Eclipta alba (L.) Hassk (Asteraceae) against chikungunya vector, Aedes aegypti (Linn.) (Diptera: Culicidae).
abstract: OBJECTIVE
  The present study deals with the investigation of larvicidal and ovicidal activities of benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of Eclipta alba (E. alba) against dengue vector, Aedes aegypti (Ae. Aegypti).
  
  
  METHODS
  Twenty five early III instar larvae of Ae. aegypti was exposed to various concentrations (50-300 ppm) and was assayed in the laboratory by using the protocol of WHO 2005; the 24 h LC(50) values of the E. alba leaf extract was determined by Probit analysis. For ovicidal activity, slightly modified method of Su and Mulla was performed. The ovicidal activity was determined against Ae. aegypti to various concentrations ranging from 100-350 ppm under the laboratory conditions. The egg hatch rates were assessed 48 h post treatment.
  
  
  RESULTS
  The LC(50) values of benzene, hexane, ethyl acetate, methanol and chloroform extract of E. alba against early third instar larvae of Ae. aegypti were 151.38, 165.10, 154.88, 127.64 and 146.28 ppm, respectively. Maximum larvicidal activity was observed in the methanol extract followed by chloroform, benzene, ethyl acetate and hexane extract. No mortality was observed in control. Among five solvent tested the methanol extract was found to be most effective for ovicidal activity against Ae. aegypti. The methanol extracts exerted 100% mortality (zero hatchability) at 300 ppm.
  
  
  CONCLUSIONS
  From the results it can be concluded the crude extract of E. alba was an excellent potential for controlling Ae. aegypti mosquito.
authors.affiliation: Division of Phytochemistry and Vector Biology, Department of Zoology, Annamalai University, Annamalai Nagar-608 002, India. drgovind1979@rediffmail.com
authors.forename: M
authors.initials: M
authors.lastname: Govindarajan
authors.forename: P
authors.initials: P
authors.lastname: Karuppannan
mesh_terms: D000330:Aedes / Q000187:drug effects*; D000818:Animals; D004199:Disease Vectors; D036601:Eclipta / Q000737:chemistry*; D007306:Insecticides / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D010936:Plant Extracts