title: [Preliminary study of osteoblastic effect in different contents of calcium silicate bioceramics].
abstract: OBJECTIVE
  Using β-TCP as the control, two kinds of bioceramics were prepared to determine whether and studied bioceramics could promote new bone formation in vitro, which will lay a foundation to further study the mechanism of new bone formation.
  
  
  METHODS
  Osteoblast-like cells from calvaria of neonatal Sprague Dawley rats (24h old) were purified and then used in this study. MTT assay demonstrated that the extracts of three kinds of materials could promote cell proliferation. Cell periods were determined by FCM. Alkaline phosphatase(ALP) activity was detected to illustrate the contents of ALP in the supernatant. SEM was used to observe cell attachment on the disc. SPSS 17.0 software package was used for data analysis.
  
  
  RESULTS
  According to MTT assay, the amounts of cell-treated extracts were increased with the growth of CS contents. The result of cell periods showed that the DNA in the period of G0/G1 of cell-treated containing CS extracts were much higher than those of β-TCP. And at the same point, the amounts of ALP raised with the increase of CS contents in a time-dependent way. SEM results found that cell attachment on the surface of discs of containing CS was better than that of pure β-TCP.There was no significant difference between 50wt% CS and 100wt% CS.
  
  
  CONCLUSIONS
  The abilities of cell proliferation and differentiation were increased with the growth of CS contents. The biological qualities of containing CS materials were better than those of β-TCP.
authors.affiliation: Shanghai Biomaterials Research and Testing Center,Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200023,Shanghai Key Laboratory of Stomatology.Shanghai 200011,China. emmanuellebeart1031@hotmail.com
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Fei
authors.forename: Jiao
authors.initials: J
authors.lastname: Sun
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002118:Calcium; D017610:Calcium Compounds; D002130:Calcium Phosphates; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics; D010006:Osteoblasts; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates
chemical_list: D017610:Calcium Compounds; D002130:Calcium Phosphates; D017640:Silicates; C485817:beta-tricalcium phosphate; D000469:Alkaline Phosphatase; C031293:calcium silicate; D002118:Calcium