title: The role of 1-methylcyclopropene in lignification and expansin gene expression in peeled water bamboo shoot (Zizania caduciflora L.).
abstract: BACKGROUND
  Water bamboo shoot (WBS) (Zizania caduciflora L.) is a fleshy aquatic vegetable susceptible to lignification. In this study, effects of 1-methylcyclopropene (1-MCP) anti-ethylene treatment on lignification of harvested peeled WBS were investigated.
  
  
  RESULTS
  Peeled shoots were treated with 0.5 µL L(-1) 1-MCP for 20 h at 20 °C and then stored at 20 °C for up to 9 days. Sensory quality, lignin content and activities of the lignification-associated enzymes peroxidase (POD) and phenylalanine ammonia lyase (PAL) were evaluated. Expression of expansin (ZcExp) was also assessed. 1-MCP application maintained better sensory quality and inhibited the increase in lignin content. Lessened lignification was associated with reduced activities of POD and PAL. Moreover, 1-MCP-treated shoots showed lower expression of the ZcExp gene.
  
  
  CONCLUSIONS
  1-MCP pretreatment suppressed the synthesis of lignin and thereby delayed lignification in peeled WBS.
authors.affiliation: Food Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Song
authors.forename: Haiyan
authors.initials: H
authors.lastname: Gao
authors.forename: Wenxuan
authors.initials: W
authors.lastname: Chen
authors.forename: Hangjun
authors.initials: H
authors.lastname: Chen
authors.forename: Jinlin
authors.initials: J
authors.lastname: Mao
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xuewu
authors.initials: X
authors.lastname: Duan
authors.forename: Daryl C
authors.initials: DC
authors.lastname: Joyce
mesh_terms: D001483:Base Sequence; D003521:Cyclopropanes / Q000494:pharmacology*; D005030:Ethylenes / Q000037:antagonists & inhibitors; D005511:Food Handling; D005520:Food Preservatives / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010858:Pigmentation / Q000187:drug effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018520:Plant Shoots / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006109:Poaceae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011786:Quality Control; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D012677:Sensation; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D005520:Food Preservatives; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C095825:expansin protein, plant; D008031:Lignin; C036216:ethylene; D009195:Peroxidase; D010650:Phenylalanine Ammonia-Lyase; C412563:1-methylcyclopropene