title: Preparation and biocompatibility of BSA monolayer on silicon surface.
abstract: This paper describes a general strategy for grafting protein molecules on silicon surface by using dopamine as adhesive layer. With this method, silicon surface had been successfully modified by BSA monolayer. Fourier transform infrared spectra, X-ray photoelectron spectroscopy, contact angle analysis and atomic force microscopy confirmed the sequential grafting of initiator and protein molecules. Cell adhesion experiments with PC-12 cells showed that the obtained monolayer exhibits good biocompatibility. The corrosion resistance behavior of the polydopamine and BSA modified silicon wafers was investigated by potentiodynamic test, which indicated that the modified surfaces exhibited a better anti-corrosion capability than silicon surface. All these results must be valuable for the application of protein monolayer in biological and biomedical technology.
authors.affiliation: School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Tao
authors.forename: Junyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Shengrong
authors.initials: S
authors.lastname: Yang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001709:Biotechnology / Q000379:methods; D002417:Cattle; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002470:Cell Survival; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D012825:Silicon / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D012710:Serum Albumin, Bovine; D004298:Dopamine; D012825:Silicon