title: Overexpression of the small GTPase Arl4D suppresses adipogenesis.
abstract: Arl4D is a developmentally-regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/Arl) family of Ras-related GTPases. Although Arl4 protein is reported to be expressed in adipose tissue, the function of Arl4D is unknown. To investigate the potential role of Arl4D in adipogenesis, we examined Arl4D expression during adipocyte differentiation and the effects of Arl4D overexpression on adipogenesis. Arl4D protein increased early in adipogenesis, with the highest expression at 4 h after adipogenesis initiation, followed by a decrease thereafter. Overexpression of Arl4D in 3T3-L1 cells potently inhibited their ability to differentiate and accumulate lipid, and reduced the expression of adipogenic genes. Furthermore, treatment with valproic acid, an Arl4D inducer, suppressed adipogenesis. These results suggest that rapid reduction of Arl4D is required for adipogenesis to proceed.
authors.affiliation: Department of Biochemistry, Research Institute for Endocrine Sciences, Chonbuk National University Medical School, Jeonju, Jeonbuk, Republic of Korea.
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yu
authors.forename: Sun-O
authors.initials: SO
authors.lastname: Ka
authors.forename: Kang-Beom
authors.initials: KB
authors.lastname: Kwon
authors.forename: Sang-Myeong
authors.initials: SM
authors.lastname: Lee
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Byung-Hyun
authors.initials: BH
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D014635:Valproic Acid; D020727:ADP-Ribosylation Factors; C552323:Arl4D protein, mouse