title: Use of cryoprotectant-depleted allogeneic peripheral blood stem cells for transplantation.
abstract: Transplantation with cryopreserved allogeneic peripheral blood stem cells (PBSCs) from related donors is widely conducted in Japan. To freeze PBSCs, a solution containing dimethyl sulfoxide (DMSO), which can have various adverse effects, is added. DMSO-depleted allogeneic PBSCs were transplanted into 21 patients. The cryoprotectant was manually removed from thawed PBSCs and the cells were mixed with a solution containing citrate dextrose as an anticoagulant and RPMI-1640 medium. DMSO-depleted PBSCs were immediately infused into patients subjected to conditioning. Infusion-related adverse effects were only observed in three patients. The median neutrophil recovery (⩾0·5×10(9)/l) and platelet recovery (⩾20×10(9)/l) were 13·0 and 14·0 days, respectively. Only one patient with mixed-lineage leukemia in non-complete remission did not show engraftment, likely due to a second transplantation and a two-antigen disparity in human leukocyte antigen system A. The results suggest the removal of DMSO from thawed PBSCs to be safe and useful for transplantation.
authors.affiliation: Division of Cell Transplantation and Transfusion, Department of Medicine, Jichi Medical University Hospital, Shimotsuke, Tochigi, Japan. yuji-hirata@jichi.ac.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirata
authors.forename: Koji
authors.initials: K
authors.lastname: Kishino
authors.forename: Fumiko
authors.initials: F
authors.lastname: Onozaki
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakaki
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Chizuru
authors.initials: C
authors.lastname: Yamamoto
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sato
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ozaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
authors.forename: Kazuo
authors.initials: K
authors.lastname: Muroi
mesh_terms: D000293:Adolescent; D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006412:Hematopoietic Stem Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous; D055815:Young Adult