title: Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet.
abstract: OBJECTIVE
  To examine in mice the acute effects of epigallocatechin gallate (EGCG), a green tea bioactive polyphenol on substrate metabolism with focus on the fate of dietary lipids.
  
  
  METHODS
  Male C57BL/6 mice were fed high-fat diets supplemented with EGCG extracted from green tea (TEAVIGO, DSM Nutritional Products Ltd, Basel, Switzerland) at different dosages up to 1% (w/w). Effects of EGCG on body composition (quantitative magnetic resonance), food intake and digestibility, oxidation and incorporation of exogenous lipids (stable isotope techniques: (13)C-labeled palmitate and diet supplemented with corn oil as a natural source of (13)C-enriched lipids) as well as gene expression (quantitative real-time PCR) in liver and intestinal mucosa were investigated.
  
  
  RESULTS
  Short-term supplementation (4-7 days) of dietary EGCG increased energy excretion, while food and energy intake were not affected. Fecal energy loss was accompanied by increased fat and nitrogen excretion. EGCG decreased post-prandial triglyceride and glycogen content in liver, increased oxidation of dietary lipids and decreased incorporation of dietary 13C-enriched lipids into fat tissues, liver and skeletal muscle. EGCG dose dependently reversed high-fat diet-induced effects on intestinal substrate transporters (CD36, FATP4 and SGLT1) and downregulated lipogenesis-related genes (ACC, FAS and SCD1) in liver in the post-prandial state.
  
  
  CONCLUSIONS
  Anti-obesity effects of EGCG can be explained by a decreased food digestibility affecting substrate metabolism of intestinal mucosa and liver, leading to increased post-prandial fat oxidation and reduced incorporation of dietary lipids into tissues.
authors.affiliation: German Institute of Human Nutrition in Potsdam-Rehbruecke, Group of Energy Metabolism, Arthur-Scheunert-Allee 114-116, Nuthetal, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Friedrich
authors.forename: K J
authors.initials: KJ
authors.lastname: Petzke
authors.forename: D
authors.initials: D
authors.lastname: Raederstorff
authors.forename: S
authors.initials: S
authors.lastname: Wolfram
authors.forename: S
authors.initials: S
authors.lastname: Klaus
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001823:Body Composition / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D059305:Diet, High-Fat; D019587:Dietary Supplements; D004435:Eating; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D060888:Real-Time Polymerase Chain Reaction; D013662:Tea
chemical_list: D000975:Antioxidants; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate