title: Changing trends in prevalence of different Plasmodium species with dominance of Plasmodium falciparum malaria infection in Aligarh (India).
abstract: OBJECTIVE
  To determine the prevalence of malaria in Aligarh and analyze species dominance in different years over a decade.
  
  
  METHODS
  Diagnosis of malaria was done using microscopy as gold standard, rapid antigen detection assays and quantitative buffy coat (QBC) assays. Giemsa stained blood smear examination was done, thick and thin films were examined for presence of different Plasmodium spp. Rapid antigen detection assays employing detection of HRP-2 and parasite lactate dehydrogenase antigen (pLDH) by immunochromatography was done in patients whose blood smear found to be negative by conventional Giemsa slide examination. QBC was done in cases where there is strong clinical suspicion of malaria with blood smear negative, in patients with chronic malaria, splenomegaly, or in those patients who had inadequate treatment and for post-treatment follow up.
  
  
  RESULTS
  Plasmodium vivax and Plasmodium falciparum were only species detected in our hospital. Overall prevalence of malaria in Aligarh was found to be 8.8%. The maximum prevalence of 20.1% was observed in year 2008 and lowest 2.3% in 2002.
  
  
  CONCLUSIONS
  High prevalence of malaria is observed in this part of country with dominance of both species particularly Plasmodium falciparum should be monitored and factors accounting for occurrence should be studied to employ effective control measures.
authors.affiliation: Department of Microbiology, JN Medical College and Hospital, AMU Aligarh.
authors.forename: Haris M
authors.initials: HM
authors.lastname: Khan
authors.forename: Fatima
authors.initials: F
authors.lastname: Shujatullah
authors.forename: Mohammad
authors.initials: M
authors.lastname: Ashfaq
authors.forename: Adil
authors.initials: A
authors.lastname: Raza
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D001769:Blood / Q000469:parasitology; D006801:Humans; D007194:India / Q000453:epidemiology; D008288:Malaria / Q000453:epidemiology* / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D010961:Plasmodium / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*; D015995:Prevalence
chemical_list: D000953:Antigens, Protozoan