title: Carbon nanotubes for voltage reduction and throughput enhancement of electrical cell lysis on a lab-on-a-chip.
abstract: We report on the enhancement of electrical cell lysis using carbon nanotubes (CNTs). Electrical cell lysis systems are widely utilized in microchips as they are well suited to integration into lab-on-a-chip devices. However, cell lysis based on electrical mechanisms has high voltage requirements. Here, we demonstrate that by incorporating CNTs into microfluidic electrolysis systems, the required voltage for lysis is reduced by half and the lysis throughput at low voltages is improved by ten times, compared to non-CNT microchips. In our experiment, E. coli cells are lysed while passing through an electric field in a microchannel. Based on the lightning rod effect, the electric field strengthened at the tip of the CNTs enhances cell lysis at lower voltage and higher throughput. This approach enables easy integration of cell lysis with other on-chip high-throughput sample-preparation processes.
authors.affiliation: University of Waterloo, 200 University Avenue West, Waterloo, ON, Canada.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shahini
authors.forename: John T W
authors.initials: JT
authors.lastname: Yeow
mesh_terms: D003470:Culture Media; D003584:Cytological Techniques / Q000295:instrumentation; D004572:Electrolysis / Q000295:instrumentation*; D004926:Escherichia coli / Q000166:cytology / Q000502:physiology; D057166:High-Throughput Screening Assays; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D003470:Culture Media; D037742:Nanotubes, Carbon