title: Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium.
abstract: Hydroxyapatite (HA)-coated titanium (Ti) is commonly used for implantable medical devices. This study examined in vitro osteoblast gene expression and antimicrobial activity against early and late colonizers of supra-gingival plaque on nanoscale HA-coated Ti prepared by discharge in a physiological buffered solution. The HA-coated Ti surface showed super-hydrophilicity, whereas the densely sintered HA and Ti surfaces alone showed lower hydrophilicity. The sintered HA and HA-coated Ti surfaces enhanced osteoblast phenotypes in comparison with the bare Ti surface. The HA-coated Ti enabled antimicrobial activity against early colonizers of supra-gingival plaques, namely Streptococcus mitis and Streptococcus gordonii. Such antimicrobial activity may be caused by the surface hydrophilicity, thereby leading to a repulsion force between the HA-coated Ti surface and the bacterial cell membranes. On the contrary, the sintered HA sample was susceptible to infection of microorganisms. Thus, hydrophilic-modified HA-coated Ti may have potential for use in implantable medical devices. From the Clinical Editor: This study establishes that Hydroxyapatite (HA)-coated titanium (Ti) surface of implanted devices may result in an optimal microenvironment to control and prevent infections and may have potential future clinical applications.
authors.affiliation: Department of Oral Biomaterials and Technology, Showa University School of Dentistry, Tokyo, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Murakami
authors.forename: Takafumi
authors.initials: T
authors.lastname: Arimoto
authors.forename: Dai
authors.initials: D
authors.lastname: Suzuki
authors.forename: Misato
authors.initials: M
authors.lastname: Iwai-Yoshida
authors.forename: Fukunaga
authors.initials: F
authors.lastname: Otsuka
authors.forename: Yo
authors.initials: Y
authors.lastname: Shibata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Igarashi
authors.forename: Ryutaro
authors.initials: R
authors.lastname: Kamijo
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests; D020527:Microscopy, Scanning Probe; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology; D010641:Phenotype; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D014025:Titanium; D010100:Oxygen