title: In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2.
abstract: 3,5-O-di-galloylquinic acid (DGQA) purified from leaves of Myrtus communis was investigated for its antioxidative, antiproliferative and antigenotoxic activities. Antioxidant activity was determined by the ability of the compound to inhibit lipid peroxidation induced by H(2)O(2) in the K562 cell line. The pure molecule displayed an important malondialdehyde formation inhibition percentage (82.2%). Moreover, this compound exhibited an inhibitory effect against H(2)O(2)-induced genotoxicity, using the comet assay. A protective effect of the same molecule was also revealed when assessing the gene expression of the chronic myelogenous leukemia cell line (K562), stressed with H(2)O(2). For this purpose, we used a cDNA-microarray containing 82 genes related to cell defense, essentially represented by antioxidant and DNA repair proteins. We found that DGQA increase the activity of antioxidant enzymes family and the activity of DNA repair enzymes. Taken together, these observations provide evidence that the DGQA is able to protect cells against oxidative stress.
authors.affiliation: Unité de Pharmacognosie, Faculté de Pharmacie de Monastir, Rue Avicenne, 5000, Monastir, Tunisia.
authors.forename: Skandrani
authors.initials: S
authors.lastname: Ines
authors.forename: Bouhlel
authors.initials: B
authors.lastname: Ines
authors.forename: Bhouri
authors.initials: B
authors.lastname: Wissem
authors.forename: Ben Sghaier
authors.initials: BS
authors.lastname: Mohamed
authors.forename: Hayder
authors.initials: H
authors.lastname: Nawel
authors.forename: Marie-Genviève
authors.initials: MG
authors.lastname: Dijoux-Franca
authors.forename: Ghedira
authors.initials: G
authors.lastname: Kamel
authors.forename: Chekir-Ghedira
authors.initials: CG
authors.lastname: Leïla
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020014:K562 Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D009152:Mutagenicity Tests; D031651:Myrtus / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D010936:Plant Extracts; D011801:Quinic Acid; D006861:Hydrogen Peroxide