title: Transcriptome profiling reveals a novel role for trichostatin A in antagonizing histone chaperone Chz1 mediated telomere anti-silencing.
abstract: The histone chaperones play an important role in chromatin assembly and disassembly during replication and transcription. We have assessed the global roles of histone chaperones in Saccharomyces cerevisiae. Microarray transcriptional analyzes indicate that histone chaperones have their own specific target genes, and various histone chaperones have partially overlapping functions during transcriptional regulation. The histone deacetylase inhibitor TSA and histone chaperones Asf1, Vps75 and Rtt106 can function in parallel pathways to regulate transcription. Moreover, TSA can specifically antagonize histone chaperone Chz1-mediated telomere anti-silencing. This study demonstrates that a mutual cross-talk mechanism exists between histone chaperones and histone deacetylation in transcriptional regulation.
authors.affiliation: The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Science, Southeast University, Nanjing, China. ywansystemsbiology@gmail.com
authors.forename: Yakun
authors.initials: Y
authors.lastname: Wan
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.forename: Jinyin
authors.initials: J
authors.lastname: Tan
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Huiying
authors.initials: H
authors.lastname: Chen
authors.forename: Zhanxian
authors.initials: Z
authors.lastname: Lin
authors.forename: Jung-Hsien
authors.initials: JH
authors.lastname: Chiang
authors.forename: Ramsey A
authors.initials: RA
authors.lastname: Saleem
authors.forename: Saleem
authors.initials: S
authors.lastname: Ramsey
mesh_terms: D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D056488:Histone Chaperones / Q000037:antagonists & inhibitors*; D006655:Histone Deacetylases; D006877:Hydroxamic Acids / Q000494:pharmacology*; D029701:Saccharomyces cerevisiae Proteins / Q000037:antagonists & inhibitors*; D016615:Telomere / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C518297:Chz1 protein, S cerevisiae; D056488:Histone Chaperones; D006877:Hydroxamic Acids; D029701:Saccharomyces cerevisiae Proteins; C012589:trichostatin A; D006655:Histone Deacetylases