title: Adverse biophysical effects of hydroxyapatite nanoparticles on natural pulmonary surfactant.
abstract: Inhaled nanoparticles (NPs) must first interact with the pulmonary surfactant (PS) lining layer that covers the entire internal surface of the respiratory tract and plays an important role in surface tension reduction and host defense. Interactions with the PS film determine the subsequent clearance, retention, and translocation of the inhaled NPs and hence their potential toxicity. To date, little is known how NPs interact with PS, and whether or not NPs have adverse effects on the biophysical function of PS. We found a time-dependent toxicological effect of hydroxyapatite NPs (HA-NPs) on a natural PS, Infasurf, and the time scale of surfactant inhibition after particle exposure was comparable to the turnover period of surfactant metabolism. Using a variety of in vitro biophysicochemical characterization techniques, we have determined the inhibition mechanism to be due to protein adsorption onto the HA-NPs. Consequently, depletion of surfactant proteins from phospholipid vesicles caused conversion of original large vesicles into much smaller vesicles with poor surface activity. These small vesicles, in turn, inhibited biophysical function of surfactant films after adsorption at the air-water interface. Cytotoxicity study found that the HA-NPs at the studied concentration were benign to human bronchial epithelial cells, thereby highlighting the importance of evaluating biophysical effect of NPs on PS. The NP-PS interaction mechanism revealed by this study may not only provide new insight into the toxicological study of nanoparticles but also shed light on the feasibility of NP-based pulmonary drug delivery.
authors.affiliation: Department of Mechanical Engineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Fan
authors.forename: Yi E
authors.initials: YE
authors.lastname: Wang
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhao
authors.forename: Joachim S C
authors.initials: JS
authors.lastname: Loo
authors.forename: Yi Y
authors.initials: YY
authors.lastname: Zuo
mesh_terms: D000327:Adsorption / Q000187:drug effects; D001688:Biological Products / Q000737:chemistry / Q000378:metabolism; D055592:Biophysical Phenomena / Q000187:drug effects*; D001980:Bronchi / Q000166:cytology; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000009:adverse effects / Q000737:chemistry; D017886:Durapatite / Q000009:adverse effects* / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D053758:Nanoparticles / Q000009:adverse effects*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D037701:Pulmonary Surfactant-Associated Protein B / Q000737:chemistry / Q000378:metabolism; D037721:Pulmonary Surfactant-Associated Protein C / Q000737:chemistry / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties; D018675:Toxicity Tests
chemical_list: D001688:Biological Products; D003603:Cytotoxins; D010743:Phospholipids; D037701:Pulmonary Surfactant-Associated Protein B; D037721:Pulmonary Surfactant-Associated Protein C; D011663:Pulmonary Surfactants; D017886:Durapatite