title: Regulation of NB-LRR-type UNI and its related signaling pathway: signaling crosstalk and methodology for quick identification of related factors.
abstract: Activation of NB-LRR-related UNI proteins by uni-1D mutation, a gain-of-function mutation of the UNI gene, induces some pathogenesis-related responses and also affects morphology through modulation of meristem activities. In a recent study we reported that the uni-1D phenotypes require cooperative action of ERECTA (ER) receptor kinase family members in UNI-expressing cells, suggesting that an intracellular signaling crosstalk between ER-family-dependent and UNI-triggered signaling pathways plays a significant role in the phenotypes. Further we recently succeeded in the establishment of a methodology for rapid identification of factors involved in the UNI function. EMS-induced causal mutations that suppress the uni-1D phenotypes could be identified using whole-genome-sequencing technologies with much less labor compared with the conventional map-based cloning method that is generally time-consuming and labor-intensive. Thus it would be now possible to intensively identify factors that play significant roles in regulation of UNI proteins and/or UNI-related signaling pathways.
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Uchida
authors.forename: Masao
authors.initials: M
authors.lastname: Tasaka
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003583:Cytokinins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018519:Meristem / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D003583:Cytokinins; D011956:Receptors, Cell Surface; C532244:UNI protein, Arabidopsis; C457763:ER protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases