title: Profiling the active site of a copper enzyme through its far-infrared fingerprint.
abstract: Vibrations of the metal active site of the Cu,Zn-superoxide dismutase enzyme were analyzed by far-infrared difference spectroscopy and theoretical normal mode calculation. Both electrochemically triggered Cu(I) and Cu(II) redox states show well-defined infrared vibrational modes, notably modes of the histidine ligands, the Cu(II)-His(61)-Zn(II) bridge and of the water pseudo-ligand.
authors.affiliation: CEA, DSV, IBEB, Lab Interact Protein Metal, CNRS, UMR Biol Veget and Microbiol Environ, Aix-Marseille Université, Saint-Paul-lez-Durance, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Marboutin
authors.forename: Hugo
authors.initials: H
authors.lastname: Petitjean
authors.forename: Bertrand
authors.initials: B
authors.lastname: Xerri
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vita
authors.forename: François
authors.initials: F
authors.lastname: Dupeyrat
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Flament
authors.forename: Dorothée
authors.initials: D
authors.lastname: Berthomieu
authors.forename: Catherine
authors.initials: C
authors.lastname: Berthomieu
mesh_terms: D020134:Catalytic Domain; D003300:Copper / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D008024:Ligands; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D013482:Superoxide Dismutase / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D008024:Ligands; D014867:Water; D006639:Histidine; D003300:Copper; D013482:Superoxide Dismutase