title: One-step process for debromination and aerobic mineralization of tetrabromobisphenol-A by a novel Ochrobactrum sp. T isolated from an e-waste recycling site.
abstract: A novel bacterium, Ochrobactrum sp. T, capable of simultaneous debromination and aerobic mineralization of tetrabromobisphenol-A (TBBPA), was isolated from a sludge sample collected from an electronic-waste recycling site. The bacterium exhibited maximal debrominase activity at pH 6.5, 35 °C, and 200 rpm in Luria-Bertani culture medium. Initial TBBPA concentration and pH had more significant effects on degradation efficiency than those of temperature and inoculum size. Degradation and debromination efficiencies of 91.8% and 86.7%, respectively, were achieved within 72 h under optimized conditions of 35 °C, pH 7.0, inoculum volume of 25 mL, and TBBPA concentration of 3 mg L⁻¹. In addition, a 35.6% decrease in total organic carbon was observed after the degradation of 5 mg L⁻¹ TBBPA for 120 h. Eight metabolic intermediates were identified during the biodegradation of TBBPA. This study is the first report to propose a one-step process for TBBPA debromination and mineralization by a single bacterial strain.
authors.affiliation: The State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. antc99@gig.ac.cn
authors.forename: Taicheng
authors.initials: T
authors.lastname: An
authors.forename: Lei
authors.initials: L
authors.lastname: Zu
authors.forename: Guiying
authors.initials: G
authors.lastname: Li
authors.forename: Shungang
authors.initials: S
authors.lastname: Wan
authors.forename: Bixian
authors.initials: B
authors.lastname: Mai
authors.forename: Po Keung
authors.initials: PK
authors.lastname: Wong
mesh_terms: D001673:Biodegradation, Environmental; D059029:Electronic Waste; D054879:Halogenation; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D042041:Ochrobactrum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D011075:Polybrominated Biphenyls / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D011075:Polybrominated Biphenyls; D012336:RNA, Ribosomal, 16S; C020806:tetrabromobisphenol A