title: Elevated expression of β1,4-galactosyltransferase-I in cartilage and synovial tissue of patients with osteoarthritis.
abstract: Osteoarthritis (OA) is considered a complex illness, characterized by cartilage degeneration, secondary synovial membrane inflammation, and subchondral bone sclerosis. Previous studies have shown β1,4-galactosylransferase-I (β1,4-GalT-I) to be a key inflammatory mediator that participates in the initiation and maintenance of inflammatory reaction in diseases. In the present study, we investigated the expression and possible biological function of β1,4-GalT-I in the cartilage and synovial tissue of OA patients. Cartilage and synovial tissue samples from OA patients and healthy controls were stained for β1,4-GalT-I. Reverse transcription-polymerase chain reaction was used to observe the expression of β1,4-GalT-I, and western blot was carried out for E-selectin. The interaction between β1,4-GalT-I and E-selectin was analyzed by double labeling immunohistochemistry and immunoprecipitation. The expression of β1,4-GalT-I increased in the cartilage and synovial tissue of OA patients compared with healthy controls. E-selectin was overexpressed and was correlated with β1,4-GalT-I in OA cartilage and synovial tissue. These data suggest that β1,4-GalT-I may play an important role in the inflammatory processes in cartilage and synovial tissue of patients with OA.
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cui
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinhui
authors.initials: X
authors.lastname: Zhu
authors.forename: Jianbo
authors.initials: J
authors.lastname: Fan
authors.forename: Guofeng
authors.initials: G
authors.lastname: Bao
authors.forename: Junjun
authors.initials: J
authors.lastname: Qiu
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D002356:Cartilage / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D019040:E-Selectin / Q000378:metabolism; D005260:Female; D005700:Galactosyltransferases / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology* / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013583:Synovial Membrane / Q000201:enzymology* / Q000276:immunology / Q000473:pathology
chemical_list: D019040:E-Selectin; D012333:RNA, Messenger; D005700:Galactosyltransferases; C493310:beta1,4-galactosyltransferase, human