title: [Effect of lycium bararum polysaccharides on angiotensin II-induced senescence of human umbilical vein endothelial cells and expressions of P53 and P16].
abstract: OBJECTIVE
  To investigate the role of lycium bararum polysaccharides (LBP) on angiotensin II (AngII)-induced senescence of human umbilical vein endothelial cells (HUVECs) and expressions of P53 and P16 and explore the mechanism of LBP against aging.
  
  
  METHODS
  HUVECs cultured in vitro were stimulated with 1×10(-6) mmol/L AngII to induce cell senescence, which was identified using β-gal staining. Flow cytometry was used for analyzing the cell cycle changes, and the cell viability was assessed using CCK-8 method. Western blotting was employed to detect the expression of P53 and P16 in the exposed cells.
  
  
  RESULTS
  Compared with the control cells, the cells positive for β-gal staining was significantly increased in AngII group, and showed cell cycle arrest at G(0)/G(1) phase with decreased S-phase cell percentage and cell viability. The expression levels of P53 and P16 were significantly increased in the cells with AngII exposure (P<0.05). LBP treatment of AngII-exposed cells resulted in decreased β-gal-positive cells with a reduction in G(0)/G(1) phase cells and an increase in S phase cells. LBP treatment also increased the cell viability and significantly decreased the expression levels of P53 and P16 (P<0.05).
  
  
  CONCLUSIONS
  LBP can delay AngII-induced aging of HUVECs possibly by down-regulating the expression of P53 and P15.
authors.affiliation: Fifth Division of Cadres Ward, Guangzhou General Hospital of Guangzhou Command, Guangzhou 510010, China. lldoctor@163.com
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Xue-ni
authors.initials: XN
authors.lastname: Wang
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.forename: Lu-ni
authors.initials: LN
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ju-xiang
authors.initials: JX
authors.lastname: Feng
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004365:Drugs, Chinese Herbal; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C500223:lycium barbarum polysaccharide; D000804:Angiotensin II