title: Functionalized organic nanotubes as tubular nonviral gene transfer vector.
abstract: Tubular nanomaterials are expected to represent a novel nonviral gene transfer vectors due to the unique morphology and potential biological functionalities. Here we rationally constructed functionalized organic nanotubes (ONTs) for gene delivery through the co-assembly of bipolar glycolipid, arginine-lipid and PEG-lipid. The arginine- and PEG-functionalized ONTs efficiently formed complexes with plasmid DNA without aggregation, and protect DNA from enzymatic degradation; while the arginine-functionalized ONTs aggregated with DNA as large bundles. Long ONTs exceeding 1μm in length was rarely taken up into the cells, while those with a length of 400-800nm could effectively deliver plasmid DNA into cells and induce high transgene expression of green fluorescense protein. This study demonstrated the usefulness of functionalized ONT in gene delivery, and the functionalized ONT represents a novel type of tubular nonviral gene transfer vector.
authors.affiliation: Nanotube Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5, Higashi 1-1-1, Tsukuba, Ibaraki, Japan
authors.forename: Wuxiao
authors.initials: W
authors.lastname: Ding
authors.forename: Momoyo
authors.initials: M
authors.lastname: Wada
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kameta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Minamikawa
authors.forename: Toshimi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Masuda
mesh_terms: D001120:Arginine / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D007624:KB Cells; D018613:Microscopy, Confocal; D017348:Microscopy, Electron, Scanning Transmission; D043942:Nanotubes / Q000737:chemistry*; D010957:Plasmids; D013499:Surface Properties; D019076:Transgenes
chemical_list: D004337:Drug Carriers; D006017:Glycolipids; D049452:Green Fluorescent Proteins; D004247:DNA; D001120:Arginine