title: [Effect of folic acid and DNA methyltransferase 1 on cervical cancer and its precancerous lesion].
abstract: OBJECTIVE
  To explore the effect of folic acid and DNA methyltransferase 1 (DNMT1) on cervical cancer and cervix precancerous lesion.
  
  
  METHODS
  100 patients with cervix squamous cell carcinoma (SCC), 101 patients with cervical intraepithelial neoplasm (CIN) and 109 patients with cervix inflammation (CI) diagnosed by histology were included in this study. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and Western blot were used to detect the levels of serum folate, HPV16 infection and the expression of DNMT1 protein, respectively.
  
  
  RESULTS
  The average levels of serum folate were (2.60 ± 1.61) ng/ml, (3.14 ± 2.08) ng/ml and (3.32 ± 1.74) ng/ml, and the expression of DNMT1 protein were 2.40 ± 0.99, 1.88 ± 0.33 and 0.89 ± 0.29 in the group of SCC, CIN and CI, respectively. The relationship of folate levels and DNMT1 protein expression showed inverse correlation (r = -0.186, P = 0.001). The results in our study indicated that there was an additive interaction between low-level of serum folate and high-expression of DNMT1 protein related to the risk of CIN and SCC, with OR value as 2.50 (95%CI: 1.21 - 9.22) and 6.03 (95%CI: 2.79 - 21.72) respectively. The relative excess risk of interaction (RERI), attributable proportion of interaction (API) and synergy index (S) were 0.92, 0.36 and 2.59 in the CIN group while 2.47, 0.41 and 1.96 in the SCC group.
  
  
  CONCLUSIONS
  The low level of serum folate and high expression of DNMT1 protein seemed to be associated with high risk of cervical cancer and its precancerous lesion. It suggested that there might be a synergistic action between serum folate and DNMT1 in the progression of cervix carcinogenesis.
authors.affiliation: Department of Epidemiology, Shanxi Medical University, Taiyuan, China. wjtxw@yahoo.com.cn
authors.forename: Jin-tao
authors.initials: JT
authors.lastname: Wang
authors.forename: Xiao-xu
authors.initials: XX
authors.lastname: Huo
authors.forename: Ling
authors.initials: L
authors.lastname: Ding
authors.forename: Min
authors.initials: M
authors.lastname: Hao
authors.forename: Jin-rong
authors.initials: JR
authors.lastname: Fan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Wei-min
authors.initials: WM
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D005260:Female; D005492:Folic Acid / Q000097:blood*; D006801:Humans; D008875:Middle Aged; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D005492:Folic Acid; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human