title: Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets.
abstract: Pancreatic islets are known to express low levels of antioxidant enzymes compared to other tissues and are therefore vulnerable to oxidative stress. Enhancing antioxidant defense mechanisms in pancreatic islets help them to cope better with oxidative stress. Persistent hyperglycemia under diabetic condition leads to continuous generation of reactive oxygen species, and different tissues exposed to this are oxidatively damaged depending on their antioxidant defense. Since islet cells are very poor in their antioxidant defense, our interest was to assess their antioxidant profile under normal, diabetic, insulin treated diabetic and untreated diabetic condition. On one hand, antioxidant defense was measured in terms of antioxidant enzymes and antioxidant molecules while on the other, damage caused to biomolecules was estimated. Our data demonstrate that oxidative damage to all biomolecules increased in islets cultured from diabetic animals, which enhanced further in islets from untreated diabetic animals. Insulin treatment significantly improved oxidative stress profile of islets indicating that the control of hyperglycemia leads to improvement in oxidative stress profile.
authors.affiliation: Department of Zoology, University of Pune, Ganeshkhind, Pune, India.
authors.forename: Manisha A
authors.initials: MA
authors.lastname: Modak
authors.forename: Pradip B
authors.initials: PB
authors.lastname: Parab
authors.forename: Saroj S
authors.initials: SS
authors.lastname: Ghaskadbi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D017382:Reactive Oxygen Species; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse