title: Effects of HB-EGF and epiregulin on wound healing of gingival cells in vitro.
abstract: OBJECTIVE
  Gingival wound healing is important to periodontal disease and surgery. This in vitro study was conducted to assess the manner in which heparin-binding epidermal growth factor-like growth factor (HB-EGF) and epiregulin cooperatively participate in the wound-healing process in the gingival epithelial and fibroblast cells of the oral mucosa.
  
  
  METHODS
  Gingival epithelium and fibroblast were separated from gingival tissue biopsies and prepared to primary cultures. The changes in the mRNA expression were evaluated via real-time PCR. The effects on cell proliferation, migration, and repopulation were evaluated in vitro.
  
  
  RESULTS
  The different regulation of expressions of HB-EGF, epiregulin, and epidermal growth factor receptors was observed over time and with different gingival cell types. HB-EGF exerted a cell migration-inducing effect on both epithelial and fibroblast cells, whereas epiregulin did not. Both growth factors functioned as mitogens for epithelial cell proliferation, but not for fibroblast proliferation. HB-EGF strongly promoted epithelial cell repopulation and mildly promoted fibroblast repopulation, whereas epiregulin promoted only fibroblast repopulation.
  
  
  CONCLUSIONS
  These results indicated that both growth factors might function importantly in the wound-healing process of human gingival tissue via the different regulation of the expression, cell migration, proliferation, and repopulation.
authors.affiliation: Department of Oral Biology, BK21 Project, Oral Science Research Center, Yonsei University, Seoul, Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: E J
authors.initials: EJ
authors.lastname: Bak
authors.forename: J Y
authors.initials: JY
authors.lastname: Chang
authors.forename: S-T
authors.initials: ST
authors.lastname: Kim
authors.forename: W-S
authors.initials: WS
authors.lastname: Park
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Yoo
authors.forename: J-H
authors.initials: JH
authors.lastname: Cha
mesh_terms: D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000494:pharmacology; D066260:Epiregulin; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006493:Heparin / Q000032:analysis* / Q000494:pharmacology; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis* / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis; D020893:Receptor, ErbB-3 / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000502:physiology; D014945:Wound Healing / Q000502:physiology
chemical_list: D004815:Epidermal Growth Factor; D066260:Epiregulin; D066246:ErbB Receptors; D006493:Heparin; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D020893:Receptor, ErbB-3; D011956:Receptors, Cell Surface; C587880:EREG protein, human; C587883:HBEGF protein, human; C512478:EGFR protein, human; C508053:ERBB2 protein, human