title: Molecular identification and genotyping of Microsporidia in selected hosts.
abstract: The work is described by microscopic analysis, the serological analysis (IFAT) and the molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed Maličký, four New Zealand domestic rabbits, 11 sows of breed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of E ncephalitozoon cuniculi, Encephalitozoon intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those from the GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The Encephalitozoon intestinalis identified in the sample from swine showed no genetic heterogeneity.
authors.affiliation: University of Veterinary Medicine and Pharmacy In Košice, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Valencakova
authors.forename: Pavol
authors.initials: P
authors.lastname: Balent
authors.forename: Petra
authors.initials: P
authors.lastname: Ravaszova
authors.forename: Ales
authors.initials: A
authors.lastname: Horak
authors.forename: Miroslav
authors.initials: M
authors.lastname: Obornik
authors.forename: Monika
authors.initials: M
authors.lastname: Halanova
authors.forename: Beata
authors.initials: B
authors.lastname: Malcekova
authors.forename: Frantisek
authors.initials: F
authors.lastname: Novotny
authors.forename: Maria
authors.initials: M
authors.lastname: Goldova
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016819:Encephalitozoon / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000175:diagnosis / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011817:Rabbits; D013552:Swine; D014730:Veterinary Medicine / Q000379:methods*
chemical_list: D017931:DNA Primers