title: DNA separation in nanowall array chips.
abstract: A nanowall array structure was fabricated on a quartz chip as a separation matrix of DNA fragments, and a 30 s separation was realized for a mixture of DNA fragments (48.5 and 1 kbp fragments) by applying the electric voltage. A longer DNA fragment migrates faster than a shorter one in a nanowall array chip, and it is completely different from the separation of DNA based on gel electrophoresis, nanopillar chips, and nanoparticle array chips. Although the result is similar to DNA separation by entropic trapping, it could not be fully explained by entropic trapping phenomena. Direct observation of single-DNA molecular dynamics inside a nanowall array structure indicates that both confined elongation and relaxation recoiling of a DNA molecule occur, and an elongated DNA molecule migrates faster than a recoiled DNA molecule. Numerical fitting of DNA molecular dynamics reveals that the balance between times for the transverse of a DNA molecule in the nanowall array chip and the relaxation-recoiling of a DNA molecule governs the separation of DNA.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan. yasui.takao@e.mbox.nagoya-u.ac.jp
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Ryo
authors.initials: R
authors.lastname: Ogawa
authors.forename: Shingi
authors.initials: S
authors.lastname: Hashioka
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Horiike
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011791:Quartz
chemical_list: D011791:Quartz; D004247:DNA