title: MYC accelerates p21CIP-induced megakaryocytic differentiation involving early mitosis arrest in leukemia cells.
abstract: p21(CIP) is a potent cell cycle inhibitor often up-regulated in differentiation. Protooncogene MYC induces cell growth and proliferation, inhibits differentiation and represses p21(CIP). However, both molecules are involved in processes of polyploidisation, cell size increase, differentiation and senescence. It is unclear why MYC has a dual role in differentiation. We have previously shown that overexpression of p21(CIP) in K562 myeloid cells induces megakaryocytic differentiation with polyploidy. We have now investigated the requirements for p21(CIP) to block mitosis and induce differentiation in the presence of overactivated MYC. Silencing and over-expression studies showed that p21(CIP) is required to induce differentiation. However, the expression of p21(CIP) needs to be transient to irreversibly inhibit mitosis but not DNA replication, what leads to polyploidy. Transient overexpression of p21(CIP) caused early down-regulation of mitotic Cyclins and up-regulation of G1/S Cyclins D and E, changes typical of endoreplication. Interestingly, over-activation of MYC did not release the proliferative block imposed by p21(CIP) and instead, accelerated cell size increase, megakaryocytic differentiation and polyploidisation. Our data suggests that in some systems p21(CIP) takes part in a mitosis control driving MYC-induced cellular growth into differentiation.
authors.affiliation: Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
authors.forename: María J
authors.initials: MJ
authors.lastname: Muñoz-Alonso
authors.forename: Laura
authors.initials: L
authors.lastname: Ceballos
authors.forename: Gabriel
authors.initials: G
authors.lastname: Bretones
authors.forename: Pilar
authors.initials: P
authors.lastname: Frade
authors.forename: Javier
authors.initials: J
authors.lastname: León
authors.forename: Alberto
authors.initials: A
authors.lastname: Gandarillas
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D008533:Megakaryocytes / Q000166:cytology / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D011123:Polyploidy; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016271:Proto-Oncogene Proteins c-myc; D015032:Zinc