title: Quantitative phase restoration by direct inversion using the optical transfer function.
abstract: Quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. The presented method is noniterative and is based on partially coherent principles. It also takes into account the optical properties of the system and gives the phase of the object directly. The proposed method is especially suitable for application to weak phase objects, such as live and unstained biological samples but, surprisingly, has also been shown to work with comparatively strong phase objects.
authors.affiliation: Optical Bioimaging Laboratory, Division of Bioengineering, National University of Singapore (NUS), 7 Engineering Drive 1, 117576, Singapore.
authors.forename: Shan Shan
authors.initials: SS
authors.lastname: Kou
authors.forename: Laura
authors.initials: L
authors.lastname: Waller
authors.forename: George
authors.initials: G
authors.lastname: Barbastathis
authors.forename: Pierre
authors.initials: P
authors.lastname: Marquet
authors.forename: Christian
authors.initials: C
authors.lastname: Depeursinge
authors.forename: Colin J R
authors.initials: CJ
authors.lastname: Sheppard
mesh_terms: D000818:Animals; D017164:Ascaris lumbricoides / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D018625:Microscopy, Atomic Force / Q000379:methods*; D008938:Mitosis; D055620:Optical Phenomena; D019904:Polymethyl Methacrylate
chemical_list: D019904:Polymethyl Methacrylate