title: Extracellular recording of spatiotemporal patterning in response to odors in the olfactory epithelium by microelectrode arrays.
abstract: In olfactory biosensors, microelectronic sensor chips combined with biological olfactory cells are a promising platform for odor detection. In our investigation, olfactory epithelium stripped from rat was fixed on the surface of microelectrode arrays (MEAs). Electrophysiological activities of olfactory receptor neurons in intact epithelium were measured in the form of extracellular potentials. Based on multi-channel recording performance of MEA and structural and functional integrality of native olfactory epithelium, the spatiotemporal analysis was carried out to study the extracellular activity pattern of neurons in the tissue. The variation of spatiotemporal patterns corresponding to different odors displayed the signals firing image characteristic intuitionally. It is an effective method in the form of patterns for monitoring the state of tissue both in time and space domain, promoting the platform for olfactory sensing mechanism research.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, PR China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Ye
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Fenni
authors.initials: F
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods*; D008839:Microelectrodes; D009812:Odorants / Q000032:analysis*; D009831:Olfactory Mucosa / Q000737:chemistry / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000737:chemistry / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058067:Wavelet Analysis