title: SNPs of CXCR1 gene and its associations with somatic cell score in Chinese Holstein cattle.
abstract: Mastitis is one of the most prevalent diseases in dairy cattle; CXCR1 plays a key role in mastitis resistance via IL8 signaling pathway, with the CXCR1 SNPs showing a different degree of mastitis resistance. To investigate the situation of CXCR1 polymorphisms in Chinese Holstein cattle and determine the relationship between the CXCR1 SNPs and mastitis resistance, the CXCR1 SNPs in 610 Chinese Holstein cattle of 30 families were investigated using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The results showed that four SNPs, -1830A > G, -1768T > A, -344T > C, and 783C > A were detected at 5' upstream and coding region. The correlation analysis demonstrated that -1830AA, -1768TT, and -344TT correlated significantly with the lowest SCS for each site, respectively. Haplotype analysis revealed Haplo2 (ATTA) correlated significantly with the lowest SCS. These findings indicated a prospect genetic marker of mastitis resistance in dairy cattle.
authors.affiliation: Animal Science and Technology College, Yangzhou University, Yangzhou, Jiangsu, China.
authors.forename: Renjin
authors.initials: R
authors.lastname: Chen
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
authors.forename: Dejun
authors.initials: D
authors.lastname: Ji
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Mao
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zhang
authors.lastname: Hamza
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers / Q000235:genetics; D006239:Haplotypes; D007113:Immunity, Innate; D008414:Mastitis, Bovine / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D023062:Receptors, Interleukin-8A / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D023062:Receptors, Interleukin-8A