title: The Swi2-Snf2-like protein Uls1 is involved in replication stress response.
abstract: The Saccharomyces cerevisiae Uls1 belongs to the Swi2-Snf2 family of DNA-dependent ATPases and a new protein family of SUMO-targeted ubiquitin ligases. Here, we examine a physiological role of Uls1 and report for the first time its involvement in response to replication stress. We found that deletion of ULS1 in cells lacking RAD52 caused a synthetic growth defect accompanied by prolonged S phase and aberrant cell morphology. uls1Δ also progressed slower through S phase upon MMS treatment and took longer to resolve replication intermediates during recovery. This suggests an important function for Uls1 during replication stress. Consistently, cells lacking Uls1 and endonuclease Mus81 were more sensitive to HU, MMS and CPT than single mus81Δ. Interestingly, deletion of ULS1 attenuated replication stress-related defects in sgs1Δ, such as sensitivity to HU and MMS while increasing the level of PCNA ubiquitination and Rad53 phosphorylation. Importantly, Uls1 interactions with Mus81 and Sgs1 were dependent on its helicase domain. We propose that Uls1 directs a subset of DNA structures arising during replication into the Sgs1-dependent pathway facilitating S phase progression. Thus, in the absence of Uls1 other modes of replication fork processing and repair are employed.
authors.affiliation: Institute of Plant Biology, Faculty of Biological Sciences, University of Wrocław, 50-328 Wrocław, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Cal-Bakowska
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Litwin
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bocer
authors.forename: Robert
authors.initials: R
authors.lastname: Wysocki
authors.forename: Dorota
authors.initials: D
authors.lastname: Dziadkowiec
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics; D004720:Endonucleases / Q000235:genetics; D017353:Gene Deletion; D051721:Rad52 DNA Repair and Recombination Protein / Q000235:genetics; D053484:RecQ Helicases / Q000235:genetics; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D013312:Stress, Physiological; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; D044767:Ubiquitin-Protein Ligases; D004720:Endonucleases; C411624:MUS81 protein, S cerevisiae; D000251:Adenosine Triphosphatases; C090472:SGS1 protein, S cerevisiae; C506745:ULS1 protein, S cerevisiae; D004265:DNA Helicases; D053484:RecQ Helicases