title: Comparison of a novel real-time PCR assay with sequence analysis, reverse hybridization, and multiplex PCR for hepatitis B virus type B and C genotyping.
abstract: We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.
authors.affiliation: Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiu-Yu
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Hu
authors.forename: Wen-Lu
authors.initials: WL
authors.lastname: Zhang
authors.forename: Jie-Li
authors.initials: JL
authors.lastname: Hu
authors.forename: Ai-Zhong
authors.initials: AZ
authors.lastname: Zeng
authors.forename: Jin-Jun
authors.initials: JJ
authors.lastname: Guo
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Huang
authors.forename: Wei-Xian
authors.initials: WX
authors.lastname: Chen
authors.forename: You-Lan
authors.initials: YL
authors.lastname: Shan
authors.forename: Ai-Long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D005838:Genotype; D006509:Hepatitis B / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D058889:Molecular Typing / Q000379:methods; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D014773:Virology / Q000379:methods*