title: Novel regulation of PLCζ activity via its XY-linker.
abstract: The XY-linker region of somatic cell PLC (phospholipase)-β, -γ, -δ and -ε isoforms confers potent catalytic inhibition, suggesting a common auto-regulatory role. Surprisingly, the sperm PLCζ XY-linker does not mediate auto-inhibition. Unlike for somatic PLCs, the absence of the PLCζ XY-linker significantly diminishes both in vitro PIP2 (phosphatidylinositol 4,5-bisphosphate) hydrolysis and in vivo Ca2+-oscillation-inducing activity, revealing evidence for a novel PLCζ enzymatic mechanism.
authors.affiliation: Department of Obstetrics and Gynaecology, Cardiff University School of Medicine, Cardiff CF14 4XN, UK. mixosn@yahoo.com
authors.forename: Michail
authors.initials: M
authors.lastname: Nomikos
authors.forename: Khalil
authors.initials: K
authors.lastname: Elgmati
authors.forename: Maria
authors.initials: M
authors.lastname: Theodoridou
authors.forename: Athena
authors.initials: A
authors.lastname: Georgilis
authors.forename: J Raul
authors.initials: JR
authors.lastname: Gonzalez-Garcia
authors.forename: George
authors.initials: G
authors.lastname: Nounesis
authors.forename: Karl
authors.initials: K
authors.lastname: Swann
authors.forename: F Anthony
authors.initials: FA
authors.lastname: Lai
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006868:Hydrolysis; D051379:Mice; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D054801:Phosphoinositide Phospholipase C / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D011994:Recombinant Proteins; D054801:Phosphoinositide Phospholipase C; D002118:Calcium