title: Parallel selection of antibody libraries on phage and yeast surfaces via a cross-species display.
abstract: We created a cross-species display system that allows the display of the same antibody libraries on both prokaryotic phage and eukaryotic yeast without the need for molecular cloning. Using this cross-display system, a large, diverse library can be constructed once and subsequently used for display and selection in both phage and yeast systems. In this article, we performed the parallel phage and yeast selection of an antibody maturation library using this cross-display platform. This parallel selection allowed us to isolate more unique hits than single-species selection, with 162 unique clones from phage and 107 unique clones from yeast. In addition, we were able to shuttle yeast hits back to Escherichia coli cells for affinity characterization at a higher throughput.
authors.affiliation: Biologics Research, Merck Research Laboratories, Merck & Co., Inc., Palo Alto, CA 94304, USA.
authors.forename: Chirag A
authors.initials: CA
authors.lastname: Patel
authors.forename: Jinqing
authors.initials: J
authors.lastname: Wang
authors.forename: Xinwei
authors.initials: X
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Dong
authors.forename: Pingyu
authors.initials: P
authors.lastname: Zhong
authors.forename: Peter P
authors.initials: PP
authors.lastname: Luo
authors.forename: Kevin C
authors.initials: KC
authors.lastname: Wang
mesh_terms: D000915:Antibody Affinity; D001435:Bacteriophages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000276:immunology
chemical_list: D007140:Immunoglobulin Fab Fragments; D019151:Peptide Library; D011994:Recombinant Proteins