title: Molecular characterization of the Corynebacterium pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice.
abstract: BACKGROUND
  Heat shock proteins (HSPs) are important candidates for the development of vaccines because they are usually able to promote both humoral and cellular immune responses in mammals. We identified and characterized the hsp60-hsp10 bicistronic operon of the animal pathogen Corynebacterium pseudotuberculosis, a Gram-positive bacterium of the class Actinobacteria, which causes caseous lymphadenitis (CLA) in small ruminants.
  
  
  RESULTS
  To construct the DNA vaccine, the hsp60 gene of C. pseudotuberculosis was cloned in a mammalian expression vector. BALB/c mice were immunized by intramuscular injection with the recombinant plasmid (pVAX1/hsp60).
  
  
  CONCLUSIONS
  This vaccination induced significant anti-hsp60 IgG, IgG1 and IgG2a isotype production. However, immunization with this DNA vaccine did not confer protective immunity.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av, Antonio Carlos, 6627 - Pampulha, CP 486, CEP 31,270-901, Belo Horizonte- MG, Brazil. vasco@icb.ufmg.br.
authors.forename: Marcilia P
authors.initials: MP
authors.lastname: Costa
authors.forename: John A
authors.initials: JA
authors.lastname: McCulloch
authors.forename: Síntia S
authors.initials: SS
authors.lastname: Almeida
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.forename: Cristina T
authors.initials: CT
authors.lastname: Fonseca
authors.forename: Diana M
authors.initials: DM
authors.lastname: Oliveira
authors.forename: Maria Fs
authors.initials: MF
authors.lastname: Teixeira
authors.forename: Ewa
authors.initials: E
authors.lastname: Laskowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Lipinska
authors.forename: Roberto
authors.initials: R
authors.lastname: Meyer
authors.forename: Ricardo W
authors.initials: RW
authors.lastname: Portela
authors.forename: Sérgio C
authors.initials: SC
authors.lastname: Oliveira
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo