title: Increasing the efficacy of bioorthogonal click reactions for bioconjugation: a comparative study.
abstract: Raising the bar: the efficacy of bioorthogonal reactions for bioconjugation has been thoroughly evaluated in four different biological settings. Powered by the development of new biocompatible ligands, the copper-catalyzed azide-alkyne cycloaddition has brought about unsurpassed bioconjugation efficiency, and thus it holds great promise as a highly potent and adaptive tool for a broader spectrum of biological applications.
authors.affiliation: Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461, USA.
authors.forename: Christen
authors.initials: C
authors.lastname: Besanceney-Webler
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Tianqing
authors.initials: T
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: David
authors.initials: D
authors.lastname: Soriano del Amo
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Liana M
authors.initials: LM
authors.lastname: Klivansky
authors.forename: Florence L
authors.initials: FL
authors.lastname: Marlow
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000818:Animals; D001386:Azides / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D004625:Embryo, Nonmammalian / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D011134:Polysaccharides / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D015027:Zebrafish
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D000480:Alkynes; D001386:Azides; D005456:Fluorescent Dyes; D008024:Ligands; D011134:Polysaccharides; D013388:Succinimides; D001710:Biotin; D003300:Copper; D019809:Streptavidin