title: Proteomic analysis of larval integument, trachea and adult scale from the silkworm, Bombyx mori.
abstract: Multidimensional LC-tandem MS was used to investigate the protein compositions of three tissues of silkworm, Bombyx mori. A total of 162, 259, and 175 peptides from silkworm larval integument and trachea, and adult scale obtained by database search were matched to 48, 51, and 40 proteins, respectively. Forty-one cuticular proteins were identified from three tissues and covered all five cuticular protein families of silkworm. In the adult scale, all seven cuticular proteins were identified for the first time in the final pellet after SDS extraction. The majority of cuticular proteins were found in each tissue differentially, suggesting that tissue-specific cuticular proteins were involved in the building of the specialized tissues. Seventy-three non-cuticular proteins were also identified in this analysis mainly including muscular proteins, proteinases, inhibitors, transport proteins, and redox-related proteins.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, P. R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yunmin
authors.initials: Y
authors.lastname: Xu
authors.forename: Sai
authors.initials: S
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Jia
authors.forename: Xingfu
authors.initials: X
authors.lastname: Zha
authors.forename: Zhonghuai
authors.initials: Z
authors.lastname: Xiang
authors.forename: Ningjia
authors.initials: N
authors.lastname: He
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification; D034582:Integumentary System / Q000502:physiology*; D007814:Larva / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D013045:Species Specificity; D014132:Trachea / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; C047663:cuticle proteins, insects