title: A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.
abstract: To achieve the production of a thermostable DNA polymerase free from bacterial DNA contamination, we developed eukaryote-made thermostable DNA (Taq) polymerase. The novel eukaryote-made thermostable DNA polymerase resolves the problem of contaminating bacterial DNA in conventional bacterially made thermostable DNA polymerase as a result of its manufacture and incomplete purification. Using eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.
authors.affiliation: Clinical Laboratory Center, Toyama University Hospital, 2630 Sugitani Toyama 930-0194, Japan. hiniimi@med.u-toyama.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Niimi
authors.forename: Masashi
authors.initials: M
authors.lastname: Mori
authors.forename: Homare
authors.initials: H
authors.lastname: Tabata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Minami
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ueno
authors.forename: Shirou
authors.initials: S
authors.lastname: Hayashi
authors.forename: Isao
authors.initials: I
authors.lastname: Kitajima
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D019914:Taq Polymerase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D011994:Recombinant Proteins; D019914:Taq Polymerase