title: Does solid culture for tuberculosis influence clinical decision making in India?
abstract: METHODS
  Medical units at an academic tertiary referral hospital in Southern India.
  
  
  OBJECTIVE
  To investigate the impact of solid culture on Löwenstein-Jensen medium on clinical decision making.
  
  
  METHODS
  In a retrospective review of 150 culture-positive and 150 culture-negative consecutively sampled tuberculosis (TB) suspects, treatment decisions were analysed at presentation, after the availability of culture detection results and after the availability of drug susceptibility testing (DST) culture results.
  
  
  RESULTS
  A total of 124 (82.7%) culture-positive patients and 35 (23.3%) culture-negative patients started anti-tuberculosis treatment prior to receiving their culture results; 101 patients (33.7%) returned for their results; two (1.3%) initiated treatment based on positive culture and no culture-negative patients discontinued treatment. DST was performed on 119 (79.3%) positive cultures: 30 (25.2%) showed any resistance, eight (6.7%) showed multidrug resistance and one (0.84%) showed extensively drug-resistant TB. Twenty-eight patients (23.5%) returned for their DST results. Based on DST, treatment was modified in four patients (3.4%).
  
  
  CONCLUSIONS
  Using solid culture, 150 cultures need to be tested for one treatment modification and 30 for DST. The cost of the widespread application of culture will need to be balanced against its impact on treatment decisions in India.
authors.affiliation: Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Stall
authors.forename: T
authors.initials: T
authors.lastname: Rubin
authors.forename: J S
authors.initials: JS
authors.lastname: Michael
authors.forename: D
authors.initials: D
authors.lastname: Mathai
authors.forename: O C
authors.initials: OC
authors.lastname: Abraham
authors.forename: P
authors.initials: P
authors.lastname: Mathews
authors.forename: K
authors.initials: K
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: John
authors.forename: P
authors.initials: P
authors.lastname: Daley
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000995:Antitubercular Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003470:Culture Media; D003657:Decision Making; D054908:Extensively Drug-Resistant Tuberculosis / Q000175:diagnosis / Q000453:epidemiology; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012189:Retrospective Studies; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis / Q000453:epidemiology; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media