title: Potential of pmoA amplicon pyrosequencing for methanotroph diversity studies.
abstract: We analyzed the potential of pmoA amplicon pyrosequencing compared to that of Sanger sequencing with paddy soils as a model environment. We defined operational taxonomic unit (OTU) cutoff values of 7% and 18%, reflecting methanotrophic species and major phylogenetic pmoA lineages, respectively. Major lineages were already well covered by clone libraries; nevertheless, pyrosequencing provided a higher level of diversity at the species level.
authors.affiliation: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, D-35043 Marburg, Germany. luekec@mpi-marburg.mpg.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Lüke
authors.forename: Peter
authors.initials: P
authors.lastname: Frenzel
mesh_terms: D044822:Biodiversity; D016000:Cluster Analysis; D004783:Environmental Microbiology; D056186:Metagenomics / Q000379:methods*; D008772:Methylococcaceae / Q000145:classification* / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D010105:Oxygenases; C021555:methane monooxygenase