title: Detection and characterization of a new astrovirus in chicken and turkeys with enteric and locomotion disorders.
abstract: In the present paper, we report the unexpected discovery of a new virus in samples from chicken and turkey flocks with clinical disorders such as tenosynovitis, enteric problems, or runting and/or stunting-like conditions. Since 1987, several virus isolation attempts on samples from these flocks resulted in the same macroscopic characteristic lesions in embryonated specific pathogen free eggs, being mortality with bright-red discolouration of legs and wing-tips, a swollen dark-red liver and oedema. Initial work suggested the presence of an agent with characteristics of a non-enveloped RNA virus. Further work, which is described in this paper, showed that the isolated strains formed a new group of avian nephritis viruses, which is genetically and antigenically distinct from known avian astroviruses. Inoculation of a representative strain (isolate 19) of this new group of avian nephritis viruses, provisionally named avian nephritis virus-3, in specific pathogen free layer chicks resulted in diarrhoea, runting and stunting, and even mortality.
authors.affiliation: GD Animal Health Service, P.O. Box 9, 7400 , AA , Deventer , The Netherlands. j.d.wit@gddeventer.com
authors.forename: J J
authors.initials: JJ
authors.lastname: de Wit
authors.forename: G B Ten
authors.initials: GB
authors.lastname: Dam
authors.forename: J M A M van
authors.initials: JM
authors.lastname: de Laar
authors.forename: Y
authors.initials: Y
authors.lastname: Biermann
authors.forename: I
authors.initials: I
authors.lastname: Verstegen
authors.forename: F
authors.initials: F
authors.lastname: Edens
authors.forename: C C
authors.initials: CC
authors.lastname: Schrier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019350:Astroviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000662:veterinary*; D052676:Avastrovirus / Q000235:genetics*; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D017615:Enteric Nervous System / Q000473:pathology* / Q000821:virology; D005455:Fluorescent Antibody Technique; D020233:Gait Disorders, Neurologic / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D009426:Netherlands / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology / Q000473:pathology* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016036:Seroepidemiologic Studies; D013045:Species Specificity; D014422:Turkeys
chemical_list: D017931:DNA Primers