title: Noninvasive fetal genotyping of human platelet antigen-1a.
abstract: We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT.
authors.affiliation: Department of Experimental Immunohaematology, Sanquin Research Amsterdam and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands.
authors.forename: P G
authors.initials: PG
authors.lastname: Scheffer
authors.forename: A
authors.initials: A
authors.lastname: Ait Soussan
authors.forename: O J H M
authors.initials: OJ
authors.lastname: Verhagen
authors.forename: G C M L
authors.initials: GC
authors.lastname: Page-Christiaens
authors.forename: D
authors.initials: D
authors.lastname: Oepkes
authors.forename: M
authors.initials: M
authors.lastname: de Haas
authors.forename: C E
authors.initials: CE
authors.lastname: van der Schoot
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000097:blood*; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D005838:Genotype; D006801:Humans; D039661:Integrin beta3; D011247:Pregnancy / Q000097:blood; D011296:Prenatal Diagnosis / Q000379:methods*; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000175:diagnosis*
chemical_list: D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D004247:DNA