title: Ceacam1 separates graft-versus-host-disease from graft-versus-tumor activity after experimental allogeneic bone marrow transplantation.
abstract: BACKGROUND
  Allogeneic bone marrow transplantation (allo-BMT) is a potentially curative therapy for a variety of hematologic diseases, but benefits, including graft-versus-tumor (GVT) activity are limited by graft-versus-host-disease (GVHD). Carcinoembryonic antigen related cell adhesion molecule 1 (Ceacam1) is a transmembrane glycoprotein found on epithelium, T cells, and many tumors. It regulates a variety of physiologic and pathological processes such as tumor biology, leukocyte activation, and energy homeostasis. Previous studies suggest that Ceacam1 negatively regulates inflammation in inflammatory bowel disease models.
  
  
  METHODS
  We studied Ceacam1 as a regulator of GVHD and GVT after allogeneic bone marrow transplantation (allo-BMT) in mouse models. In vivo, Ceacam1(-/-) T cells caused increased GVHD mortality and GVHD of the colon, and greater numbers of donor T cells were positive for activation markers (CD25(hi), CD62L(lo)). Additionally, Ceacam1(-/-) CD8 T cells had greater expression of the gut-trafficking integrin α(4)β(7), though both CD4 and CD8 T cells were found increased numbers in the gut post-transplant. Ceacam1(-/-) recipients also experienced increased GVHD mortality and GVHD of the colon, and alloreactive T cells displayed increased activation. Additionally, Ceacam1(-/-) mice had increased mortality and decreased numbers of regenerating small intestinal crypts upon radiation exposure. Conversely, Ceacam1-overexpressing T cells caused attenuated target-organ and systemic GVHD, which correlated with decreased donor T cell numbers in target tissues, and mortality. Finally, graft-versus-tumor survival in a Ceacam1(+) lymphoma model was improved in animals receiving Ceacam1(-/-) vs. control T cells.
  
  
  CONCLUSIONS
  We conclude that Ceacam1 regulates T cell activation, GVHD target organ damage, and numbers of donor T cells in lymphoid organs and GVHD target tissues. In recipients of allo-BMT, Ceacam1 may also regulate tissue radiosensitivity. Because of its expression on both the donor graft and host tissues, this suggests that targeting Ceacam1 may represent a potent strategy for the regulation of GVHD and GVT after allogeneic transplantation.
authors.affiliation: Department of Immunology and Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America.
authors.forename: Sydney X
authors.initials: SX
authors.lastname: Lu
authors.forename: Lucy W
authors.initials: LW
authors.lastname: Kappel
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Charbonneau-Allard
authors.forename: Renée
authors.initials: R
authors.lastname: Atallah
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Holland
authors.forename: Claire
authors.initials: C
authors.lastname: Turbide
authors.forename: Vanessa M
authors.initials: VM
authors.lastname: Hubbard
authors.forename: Jimmy A
authors.initials: JA
authors.lastname: Rotolo
authors.forename: Marsinay
authors.initials: M
authors.lastname: Smith
authors.forename: David
authors.initials: D
authors.lastname: Suh
authors.forename: Christopher
authors.initials: C
authors.lastname: King
authors.forename: Uttam K
authors.initials: UK
authors.lastname: Rao
authors.forename: Nury
authors.initials: N
authors.lastname: Yim
authors.forename: Johanne L
authors.initials: JL
authors.lastname: Bautista
authors.forename: Robert R
authors.initials: RR
authors.lastname: Jenq
authors.forename: Olaf
authors.initials: O
authors.lastname: Penack
authors.forename: Il-Kang
authors.initials: IK
authors.lastname: Na
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: George
authors.initials: G
authors.lastname: Murphy
authors.forename: Onder
authors.initials: O
authors.lastname: Alpdogan
authors.forename: Richard S
authors.initials: RS
authors.lastname: Blumberg
authors.forename: Fernando
authors.initials: F
authors.lastname: Macian
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Holmes
authors.forename: Nicole
authors.initials: N
authors.lastname: Beauchemin
authors.forename: Marcel R M
authors.initials: MR
authors.lastname: van den Brink
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D002272:Carcinoembryonic Antigen / Q000378:metabolism*; D016764:Cell Polarity / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D003602:Cytotoxicity, Immunologic / Q000528:radiation effects; D003713:Dendritic Cells / Q000276:immunology / Q000528:radiation effects; D006086:Graft vs Host Disease / Q000150:complications / Q000276:immunology* / Q000401:mortality; D020350:Graft vs Tumor Effect / Q000276:immunology* / Q000528:radiation effects; D006801:Humans; D016023:Integrins / Q000378:metabolism; D007421:Intestine, Small / Q000473:pathology / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000276:immunology / Q000528:radiation effects; D018655:Lymphocyte Count; D008221:Lymphoid Tissue / Q000166:cytology / Q000528:radiation effects; D051379:Mice; D009928:Organ Specificity / Q000276:immunology / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000150:complications / Q000378:metabolism / Q000473:pathology; D011839:Radiation, Ionizing; D014184:Transplantation, Homologous
chemical_list: D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D016023:Integrins; C071777:integrin alpha4beta7