title: Synthetic poliovirus and other designer viruses: what have we learned from them?
abstract: Owing to known genome sequences, modern strategies of DNA synthesis have made it possible to recreate in principle all known viruses independent of natural templates. We describe the first synthesis of a virus (poliovirus) in 2002 that was accomplished outside living cells. We comment on the reaction of laypeople and scientists to the work, which shaped the response to de novo syntheses of other viruses. We discuss those viruses that have been synthesized since 2002, among them viruses whose precise genome sequence had to be established by painstakingly stitching together pieces of sequence information, and viruses involved in zoonosis. Synthesizing viral genomes provides a powerful tool for studying gene function and the pathogenic potential of these organisms. It also allows modification of viral genomes to an extent hitherto unthinkable. Recoding of poliovirus and influenza virus to develop new vaccine candidates and refactoring the phage T7 DNA genome are discussed as examples.
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11790, USA. ewimmer@ms.cc.sunysb.edu
authors.forename: Eckard
authors.initials: E
authors.lastname: Wimmer
authors.forename: Aniko V
authors.initials: AV
authors.lastname: Paul
mesh_terms: D017123:Bacteriophage T7 / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D004279:DNA, Viral / Q000138:chemical synthesis* / Q000235:genetics; D005813:Genes, Synthetic; D016679:Genome, Viral; D006801:Humans; D009975:Orthomyxoviridae / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D017955:Poliovirus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D012367:RNA, Viral / Q000138:chemical synthesis* / Q000235:genetics; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral