title: Presence of c-kit mRNA in goat ovaries and improvement of in vitro preantral follicle survival and development with kit ligand.
abstract: This study evaluated the levels of c-kit mRNA in goat follicles and the effects of kit ligand (KL) on the in vitro development of cultured preantral follicles. Preantral follicles isolated from goat ovarian cortex were cultured for 18 days in α-MEM(+) supplemented with KL (0, 50 or 100 ng/mL) in the absence or presence of follicle stimulating hormone (FSH). Real-time PCR showed that c-kit mRNA was higher in primordial and primary follicles than in secondary stage. Regarding the culture, KL addition in the absence of FSH improved the follicular survival, antrum formation, oocyte growth and meiotic resumption. KL-positive effects were not observed in the presence of FSH. In conclusion, c-kit mRNAs are detected in all follicular categories. KL promotes the survival and antral cavity formation of caprine preantral follicles after in vitro culture, as well as the growth and meiotic resumption of their oocytes in the absence of FSH.
authors.affiliation: Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles, State University of Ceará, Fortaleza, CE, Brazil. dora.uece@gmail.com
authors.forename: I M T
authors.initials: IM
authors.lastname: Lima
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.forename: G Q
authors.initials: GQ
authors.lastname: Rodrigues
authors.forename: C M G
authors.initials: CM
authors.lastname: Silva
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.forename: L F
authors.initials: LF
authors.lastname: Lima
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005453:Fluorescence; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006041:Goats / Q000235:genetics* / Q000254:growth & development*; D006801:Humans; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D019089:Stem Cell Factor / Q000494:pharmacology*; D046509:Tissue Culture Techniques; D014022:Tissue Survival / Q000187:drug effects*
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D019089:Stem Cell Factor; D005640:Follicle Stimulating Hormone; D019009:Proto-Oncogene Proteins c-kit