title: Protein unfolding studied by fluorescence methods and electrical impedance spectroscopy: the cases of Cratylia mollis and Concanavalin A.
abstract: This work is dedicated to the investigation of the prevailing molecular interactions between Cratylia mollis (Cramoll) and Concanavalin A (Con A) lectins and ionic (sodium dodecylsulfate, SDS) and non-ionic (Triton X-100, TX-100) surfactants, where we have used electrical impedance spectroscopy to map the dielectric characteristics of mixed lectin/surfactant solutions. The disorder induced in the lectin conformation is proportional to the extent of the access of the surfactant to the fluorophore present in the protein, resulting in its progressive unfolding. The complete unfolding of the lectin is associated to the formation of micelles in the core of the protein, each one of them containing a large number of detergent molecules, and therefore the process can be accompanied by measuring the electrical response of the binary surfactant/lectin system. For instance, the change in the real part of the impedance as a function of the relative concentration of the surfactant in the binary solution exhibits a breaking in its linear behavior that can be taken as indicative of a qualitative change in the environment surrounding the protein residue. We consider these results strong evidence in favor of using impedance spectroscopy methods for the analysis of protein-surfactant associations and for the characterization of the interactions that must prevail when the protein unfolds as the relative surfactant concentration is increased in aqueous solutions of these binary systems.
authors.affiliation: Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE, Brazil. csrandrade@gmail.com
authors.forename: Cesar A S
authors.initials: CA
authors.lastname: Andrade
authors.forename: Helinando P
authors.initials: HP
authors.lastname: de Oliveira
authors.forename: Maria D L
authors.initials: MD
authors.lastname: Oliveira
authors.forename: Maria T S
authors.initials: MT
authors.lastname: Correia
authors.forename: Luana C B B
authors.initials: LC
authors.lastname: Coelho
authors.forename: Celso P
authors.initials: CP
authors.lastname: de Melo
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods*; D017097:Electric Impedance; D017830:Octoxynol / Q000737:chemistry; D058767:Protein Unfolding; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D013501:Surface-Active Agents; D003208:Concanavalin A; D012967:Sodium Dodecyl Sulfate; D017830:Octoxynol