title: [Studies on anti-tumor metastatic constituents from Ardisia Crenata].
abstract: OBJECTIVE
  To study the anti-tumor metastatic constituents from Ardisia Crenata.
  
  
  METHODS
  Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.
  
  
  RESULTS
  Nine compounds(1-9) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: 5-hydroxymethyl-2-furalclehyde(1), ethyl-beta-D-fructopyranoside(2), syringic acid(3), n-butyl-beta-D-fructofuranoside(4), n-butyl-alpha-D-fructofuranoside(5), methyl-alpha-D-fructofuranoside(6), (+)-bergenin(7), ardisiacrispins B(8), asperuloside acid(9). The isolated compounds(1-9) showed positive anti-tumor metastatic activities, and compounds 1, 5, and 8 showed significant anti-tumor metastatic activities. At the concentration of 0.8 mg x L(-1), compound 5 revealed the value of metastatic inhibition ratio on MDA-MB-231 was 93.8%.
  
  
  CONCLUSIONS
  Compounds 2-6 and 9 were isolated from this plant for the first time. compounds 1, 5 and 8 showed significant anti-tumor metastatic activities.
authors.affiliation: School of Parmaceutical Sciences, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Shengan
authors.initials: S
authors.lastname: Tang
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhai
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D031644:Ardisia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D009362:Neoplasm Metastasis / Q000188:drug therapy*
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal