title: Physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp.
abstract: Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities.
authors.affiliation: Ministry of Agriculture/Bio-tech Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yi
authors.forename: Sen Tai
authors.initials: ST
authors.lastname: Liao
authors.forename: Ming Wei
authors.initials: MW
authors.lastname: Zhang
authors.forename: John
authors.initials: J
authors.lastname: Shi
authors.forename: Rui Fen
authors.initials: RF
authors.lastname: Zhang
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Deng
authors.forename: Zhen Cheng
authors.initials: ZC
authors.lastname: Wei
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D029631:Sapindaceae / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D011506:Proteins