title: [Controlling effect of antagonist bioorganic fertilizer on tomato root-knot nematode].
abstract: Indoor in vitro culture experiment and greenhouse pot experiment were conducted to evaluate the capabilities of three bacterial strains XZ-173 (Bacillus amyloliquefaciens), SL-25 (B. gibsonii), and KS-62 (Paenibacillus polymyxa) that can hydrolyze collagen protein in controlling tomato root-knot nematode. In the in vitro culture experiment, suspensions of XZ-173, SL-25, and KS-62 induced a mortality rate of 75.9%, 66.7%, and 50.0% to the second-stage junior nematode within 24 h, and decreased the egg hatching rate to 17.8%, 28.9% and 37.6% after 7-day incubation, respectively, in contrast to the 17.4% mortality rate and 53.6% egg hatching rate in the control (sterilized water). In the greenhouse pot experiment, the bioorganic fertilizer mixed with equal parts of fermented XZ-173, SL-25, and KS-62 gained the best result, with the root-knot nematode population in rhizosphere soil decreased by 84.0% as compared with the control. The bioorganic fertilizer also decreased the numbers of galls and eggs on tomato roots significantly, and increased the underground and aboveground biomass of tomato. Therefore, antagonist bioorganic fertilizer has promising potential in controlling root-knot nematode.
authors.affiliation: Jiangsu Province Key Laboratory for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China. zzenabcd@126.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Tong-jian
authors.initials: TJ
authors.lastname: Xiao
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
authors.forename: Xing-ming
authors.initials: XM
authors.lastname: Yang
authors.forename: Qi-rong
authors.initials: QR
authors.lastname: Shen
mesh_terms: D000383:Agriculture / Q000379:methods; D000818:Animals; D001407:Bacillus / Q000502:physiology*; D004780:Environment, Controlled; D005308:Fertilizers; D018551:Solanum lycopersicum / Q000254:growth & development / Q000469:parasitology*; D009348:Nematoda / Q000254:growth & development*; D010572:Pest Control, Biological; D018517:Plant Roots / Q000254:growth & development / Q000469:parasitology*
chemical_list: D005308:Fertilizers