title: CMOS image sensor for detection of interferon gamma protein interaction as a point-of-care approach.
abstract: Complementary metal oxide semiconductor (CMOS)-based image sensors have received increased attention owing to the possibility of incorporating them into portable diagnostic devices. The present research examined the efficiency and sensitivity of a CMOS image sensor for the detection of antigen-antibody interactions involving interferon gamma protein without the aid of expensive instruments. The highest detection sensitivity of about 1 fg/ml primary antibody was achieved simply by a transmission mechanism. When photons are prevented from hitting the sensor surface, a reduction in digital output occurs in which the number of photons hitting the sensor surface is approximately proportional to the digital number. Nanoscale variation in substrate thickness after protein binding can be detected with high sensitivity by the CMOS image sensor. Therefore, this technique can be easily applied to smartphones or any clinical diagnostic devices for the detection of several biological entities, with high impact on the development of point-of-care applications.
authors.affiliation: College of Bionanotechnology, Kyungwon University, San 65, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do 461 701, Republic of Korea.
authors.forename: Mohana
authors.initials: M
authors.lastname: Marimuthu
authors.forename: Karthikeyan
authors.initials: K
authors.lastname: Kandasamy
authors.forename: Chang Geun
authors.initials: CG
authors.lastname: Ahn
authors.forename: Gun Yong
authors.initials: GY
authors.lastname: Sung
authors.forename: Min-Gon
authors.initials: MG
authors.lastname: Kim
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D008670:Metals / Q000737:chemistry; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000737:chemistry; D019095:Point-of-Care Systems; D012666:Semiconductors; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D008670:Metals; D010087:Oxides; D007371:Interferon-gamma