title: Rapid repair of rat sciatic nerve injury using a nanosilver-embedded collagen scaffold coated with laminin and fibronectin.
abstract: OBJECTIVE
  Scaffold with micro-channels has shown great promise in facilitating axonal regeneration after peripheral nerve injury. Significant research has focused on mimicking, in terms of composition and function, the ability of the basement membrane of Schwann cells to both promote and guide axonal regeneration. We aim to investigate the ability of a tissue-engineered scaffold with nanosilver and collagen to adsorb laminin and fibronectin, and the usefulness of this scaffold for repairing and regenerating a 10-mm peripheral nerve gap in rats.
  
  
  METHODS
  In this study, nanosilver-embedded collagen scaffolds were prepared and coated with laminin (LN) or LN plus fibronectin (FN). Scanning electron microscopy of the transverse and longitudinal sections of the scaffold revealed axially oriented microtubules ranging from 20 to 80 µm in diameter, and the internal surface of microtubules was found to be evenly coated with LN and FN. Energy dispersive spectrometry also confirmed an even distribution of nanosilver particles within the scaffold. To test its effectiveness in restoring neuronal connection, the scaffold was used in order to bridge 10 mm gaps in the severed sciatic nerve of rats. The rats were divided into an experimental group (receiving scaffold coated with LN and FN), a control group (receiving scaffold coated with LN only) and an autologous graft group. The functional recovery 40 days after surgery was examined by electrophysiology and sciatic nerve functional index (SFI) evaluation. FluoroGold™ (FG) retrograde tracing, toluidine blue staining and transmission electron microscopy were also used to examine the regenerated nerve fibers and to establish their myelination status.
  
  
  RESULTS
  The experimental group displayed partially restored nerve function. The recovery was comparable to the effect of autologous nerve graft and was better than that observed in the control group. A better functional recovery correlated with more FG-labeled neurons, higher density of toluidine blue stained nerve fibers and thicker myelin sheath.
  
  
  CONCLUSIONS
  Our results demonstrated that nanosilver-embedded collagen scaffolds with LN and FN coating is effective in aiding axonal regeneration, and recovery is comparable to the effect of an autologous nerve graft.
authors.affiliation: Institute of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Ding
authors.forename: William W
authors.initials: WW
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhao yang
authors.initials: Zy
authors.lastname: Li
authors.forename: Hao bo
authors.initials: Hb
authors.lastname: Pan
authors.forename: Zhuojing
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology*; D007797:Laminin / Q000494:pharmacology*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology / Q000648:ultrastructure; D012834:Silver / Q000494:pharmacology*; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry; D014048:Tolonium Chloride / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D007797:Laminin; D014048:Tolonium Chloride; D012834:Silver; D003094:Collagen