title: Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans.
abstract: Endurance and strength training are established as distinct exercise modalities, increasing either mitochondrial density or myofibrillar units. Recent research, however, suggests that mitochondrial biogenesis is stimulated by both training modalities. To test the training "specificity" hypothesis, mitochondrial respiration was studied in permeabilized muscle fibers from 25 sedentary adults after endurance (ET) or strength training (ST) in normoxia or hypoxia [fraction of inspired oxygen (Fi(O(2))) = 21% or 13.5%]. Biopsies were taken from the musculus vastus lateralis, and cycle-ergometric incremental maximum oxygen uptake (VO(2max)) exercise tests were performed under normoxia, before and after the 10-wk training program. The main finding was a significant increase (P < 0.05) of fatty acid oxidation capacity per muscle mass, after endurance and strength training under normoxia [2.6- and 2.4-fold for endurance training normoxia group (ET(N)) and strength training normoxia group (ST(N)); n = 8 and 3] and hypoxia [2.0-fold for the endurance training hypoxia group (ET(H)) and strength training hypoxia group (ST(H)); n = 7 and 7], and higher coupling control of oxidative phosphorylation. The enhanced lipid oxidative phosphorylation (OXPHOS) capacity was mainly (87%) due to qualitative mitochondrial changes increasing the relative capacity for fatty acid oxidation (P < 0.01). Mitochondrial tissue-density contributed to a smaller extent (13%), reflected by the gain in muscle mass-specific respiratory capacity with a physiological substrate cocktail (glutamate, malate, succinate, and octanoylcarnitine). No significant increase was observed in mitochondrial DNA (mtDNA) content. Physiological OXPHOS capacity increased significantly in ET(N) (P < 0.01), with the same trend in ET(H) and ST(H) (P < 0.1). The limitation of flux by the phosphorylation system was diminished after training. Importantly, key mitochondrial adaptations were similar after endurance and strength training, regardless of normoxic or hypoxic exercise. The transition from a sedentary to an active lifestyle induced muscular changes of mitochondrial quality representative of mitochondrial health.
authors.affiliation: Dept. of Radiology, Univ. Clinic of Radiology I, Innsbruck Medical Univ., Anichstrasse 35, 6020 Innsbruck, Austria. dominik.pesta@i-med.ac.at
authors.forename: Dominik
authors.initials: D
authors.lastname: Pesta
authors.forename: Florian
authors.initials: F
authors.lastname: Hoppel
authors.forename: Christian
authors.initials: C
authors.lastname: Macek
authors.forename: Hubert
authors.initials: H
authors.lastname: Messner
authors.forename: Martin
authors.initials: M
authors.lastname: Faulhaber
authors.forename: Conrad
authors.initials: C
authors.lastname: Kobel
authors.forename: Walther
authors.initials: W
authors.lastname: Parson
authors.forename: Martin
authors.initials: M
authors.lastname: Burtscher
authors.forename: Michael
authors.initials: M
authors.lastname: Schocke
authors.forename: Erich
authors.initials: E
authors.lastname: Gnaiger
mesh_terms: D000328:Adult; D001706:Biopsy; D019069:Cell Respiration / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000503:physiopathology*; D050356:Lipid Metabolism / Q000502:physiology; D008931:Mitochondria, Muscle / Q000502:physiology*; D018482:Muscle, Skeletal / Q000473:pathology; D010766:Phosphorylation / Q000502:physiology; D010807:Physical Endurance / Q000502:physiology*; D055070:Resistance Training; D057185:Sedentary Behavior
chemical_list: D005227:Fatty Acids