title: Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of clostridium difficile strains.
abstract: This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.
authors.affiliation: ER8, Université Pierre et Marie Curie, Paris, France. catherine.eckert@sat.aphp.fr
authors.forename: C
authors.initials: C
authors.lastname: Eckert
authors.forename: J
authors.initials: J
authors.lastname: Van Broeck
authors.forename: P
authors.initials: P
authors.lastname: Spigaglia
authors.forename: B
authors.initials: B
authors.lastname: Burghoffer
authors.forename: M
authors.initials: M
authors.lastname: Delmée
authors.forename: P
authors.initials: P
authors.lastname: Mastrantonio
authors.forename: F
authors.initials: F
authors.lastname: Barbut
mesh_terms: D000328:Adult; D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity