title: Protective effects of peoniflorin against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells.
abstract: Peoniflorin (PF), extracted from the root of Paeonia lactiflora Pall., has been reported to have anti-inflammation and antioxidant effects in several animal models. Herein, we investigated the protective effects of PF against hydrogen peroxide (H(2)O(2))-induced oxidative damage in human umbilical vein endothelial cells (HUVECs). HUVECs were treated by H(2)O(2) (240 µmol/L) with or without PF. PF significantly increased the percent cell viability of HUVECs injured by H(2)O(2) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. By flow cytometric analysis, PF markedly attenuated H(2)O(2)-induced apoptosis and intracellular reactive oxygen species production. In addition, PF also displayed a dose-dependent reduction of lactate dehydrogenase leakage, malondialdehyde formation, and caspase-3 proteolytic activities in H(2)O(2)-treated cells, which was accompanied with a restoration of the activities of endogenous antioxidants, including total superoxide dismutase and glutathione peroxidase. Finally, Western blot data revealed that H(2)O(2) upregulated phosphorylation of extracellular signal-regulated kinase 1/2 in HUVECs, which was almost completely reversed by PF. Taken together, our data provide the first evidence that PF has a protective ability against oxidative damage in HUVECs. PF may be a candidate medicine for the treatment of vascular diseases associated with oxidative stress.
authors.affiliation: Department of Dermatovenereology, West China Hospital of Sichuan University, Chengdu, Sichuan Province, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Zai-Pei
authors.initials: ZP
authors.lastname: Guo
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Jiao
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Li
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001565:Benzoates / Q000494:pharmacology*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D039821:Monoterpenes; D018384:Oxidative Stress / Q000187:drug effects*; D029596:Paeonia; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014471:Umbilical Veins / Q000187:drug effects; D006027:Glycosides
chemical_list: D000975:Antioxidants; D001565:Benzoates; D001952:Bridged-Ring Compounds; D053148:Caspase 3; D005960:Glucosides; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D008315:Malondialdehyde; D048052:Mitogen-Activated Protein Kinase 3; D039821:Monoterpenes; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; C015423:peoniflorin; D006027:Glycosides