title: Deep and fast live imaging with two-photon scanned light-sheet microscopy.
abstract: We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.
authors.affiliation: California Institute of Technology, Beckman Institute, Pasadena, CA, USA.tvt@caltech.edu
authors.forename: Thai V
authors.initials: TV
authors.lastname: Truong
authors.forename: Willy
authors.initials: W
authors.lastname: Supatto
authors.forename: David S
authors.initials: DS
authors.lastname: Koos
authors.forename: John M
authors.initials: JM
authors.lastname: Choi
authors.forename: Scott E
authors.initials: SE
authors.lastname: Fraser
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008027:Light / Q000009:adverse effects; D008853:Microscopy / Q000379:methods*; D015027:Zebrafish