title: Notch1 expression, which is related to p65 Status, is an independent predictor of prognosis in colorectal cancer.
abstract: OBJECTIVE
  Notch1 has been proven to be aberrantly expressed in colorectal cancer and related to tumor differentiation status. However, few previous studies concentrated on the predictive role of Notch1 expression on the overall survival of patients with colorectal cancer. This study explored expression of Notch1 and its relationship with p65 and prognosis in colorectal cancer.
  
  
  METHODS
  Two independent study cohorts were involved in the present study. Clinical specimens from 941 eligible patients were constructed into tissue microarrays. The expression of Notch1 and p65 protein was investigated by immunohistochemistry.
  
  
  RESULTS
  Statistically significant positive correlations were found between protein expression of Notch1 and p65 in both retrospective and prospective study cohorts. Patients with higher Notch1 expression showed a trend of having shorter survival time, whereas patients with lower Notch1 expression had better survival in both study cohorts. In multivariate analysis, Notch1 expression was proven to be an independent predictor of prognosis. Moreover, the prognostic value of Notch1 might differ according to p65 status.
  
  
  CONCLUSIONS
  Notch1 is an independent predictor of prognosis for patients with colorectal cancer. In addition, the predictive role of Notch1 on clinical outcome might be modified by p65 status, suggesting that targeting Notch1 and nuclear factor κB (NF-κB) might be a promising strategy for colorectal cancer treatment.
authors.affiliation: Department of Gastrointestinal Surgery, Xijing Hospital, Tianjin, PR China. chudake@hotmail.com
authors.forename: Dake
authors.initials: D
authors.lastname: Chu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zixi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yuming
authors.initials: Y
authors.lastname: Li
authors.forename: Jipeng
authors.initials: J
authors.lastname: Li
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zheng
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.forename: Qingchuan
authors.initials: Q
authors.lastname: Zhao
authors.forename: Weizhong
authors.initials: W
authors.lastname: Wang
authors.forename: Rui'an
authors.initials: R
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Ji
mesh_terms: D000368:Aged; D002352:Carrier Proteins / Q000378:metabolism*; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies; D051881:Receptor, Notch1 / Q000378:metabolism*; D012189:Retrospective Studies; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D002352:Carrier Proteins; C484020:HSAJ2425 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D016328:NF-kappa B; C497761:NOTCH1 protein, human; D009363:Neoplasm Proteins; D051881:Receptor, Notch1