title: Comparative drug susceptibility study of five clonal strains of Trichomonas vaginalis in vitro.
abstract: OBJECTIVE
  To produce comparative data on a group of Trichomonas vaginalis clonal strains with varied drug responses using identical methods and materials.
  
  
  METHODS
  Five clonal strains of Trichomonas vaginalis were isolated from reference strain using agar plate technique. The variability of growth kinetic and susceptibility of clonal strain to metronidazole, tinidazole, satranidazole and nitazoxanide were observed in 96 well microtitre plate.
  
  
  RESULTS
  Among these clonal strains there was a good correlation between rates of growth with the relative susceptibility of the strains to drugs in vitro. Regarding metronidazole, tinidazole and satranidazole susceptibility, different degrees of susceptibility were determined. However, no difference in nitazoxanide susceptibility was found between the clonal strain tested and a reference strain.
  
  
  CONCLUSIONS
  This is the first description of biological variability in clonal stock of Trichomonas vaginalis. Different degrees of drug susceptibility were determined among clonal strains tested. Further studies will be necessary to ascertain the importance of this variability in clinical infection.
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Punjab, India. hemant_chaudhari5@yahoo.com
authors.forename: Hemantkumar Somabhai
authors.initials: HS
authors.lastname: Chaudhari
authors.forename: Prati Pal
authors.initials: PP
authors.lastname: Singh
mesh_terms: D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D006801:Humans; D009574:Nitro Compounds; D021261:Parasitic Sensitivity Tests; D013844:Thiazoles / Q000494:pharmacology*; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D001393:Azoles; D009574:Nitro Compounds; D013844:Thiazoles; C041747:nitazoxanide