title: Hyaluronan-modified magnetic nanoclusters for detection of CD44-overexpressing breast cancer by MR imaging.
abstract: We fabricated hyaluronan-modified magnetic nanoclusters (HA-MNCs) for detection of CD44-overexpressing breast cancer using magnetic resonance (MR) imaging. CD44 is closely associated with cancer growth, including proliferation, metastasis, invasion, and angiogenesis. Hence, pyrenyl hyaluronan (Py-HA) conjugates were synthesized as CD44-targetable surfactants with hyaluronan (HA) and 1-pyrenylbutyric acid (Py) to modify hyaluronan on hydrophobic magnetic nanocrystals. Subsequently, HA-MNCs were fabricated using the nano-emulsion method; magnetic nanocrystals were simultaneously self-assembled with Py-HA conjugates, and their physical and magnetic properties depended on the degree of substitution (DS) of Py in Py-HA conjugates. HA-MNCs exhibited superior targeting efficiency with MR sensitivity as well as excellent biocompatibility through in vitro/in vivo studies. This suggests that HA-MNCs can be a potent cancer specific molecular imaging agent via targeted detection of CD44 with MR imaging.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul 120-749, South Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: Hyun-Ouk
authors.initials: HO
authors.lastname: Kim
authors.forename: Eunji
authors.initials: E
authors.lastname: Jang
authors.forename: Joseph
authors.initials: J
authors.lastname: Park
authors.forename: Kwangyeol
authors.initials: K
authors.lastname: Lee
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D059346:Magnets / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013696:Temperature; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D018960:Hyaluronan Receptors; D008823:Micelles; D006820:Hyaluronic Acid