title: Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil.
abstract: Viral meningitis is a common infectious disease of the central nervous system (CNS) that occurs worldwide. The aim of this study was to identify the etiologic agent of lymphomonocytary meningitis in Curitiba, PR, Brazil. During the period of July 2005 to December 2006, 460 cerebrospinal fluid (CSF) samples with lymphomonocytary meningitis were analyzed by PCR methodologies. Fifty nine (12.8%) samples were positive. Enteroviruses was present in 49 (83%) samples and herpes virus family in 10 (17%), of these 6 (10%) herpes simplex virus, 1 (2%) Epstein Barr virus, 2 (3%) human herpes virus type 6 and 1 (2%) mixed infection of enterovirus and Epstein Barr virus. As conclusion enterovirus was the most frequent virus, with circulation during summer and was observed with higher frequency between 4 to 17 years of age. PCR methodology is an important method for rapid detection of RNA enterovirus and DNA herpesvirus in CSF.
authors.affiliation: Virology Laboratory, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Luine Rosele Renaud
authors.initials: LR
authors.lastname: Vidal
authors.forename: Sérgio Monteiro de
authors.initials: SM
authors.lastname: Almeida
authors.forename: Iara José de
authors.initials: IJ
authors.lastname: Messias-Reason
authors.forename: Meri Bordignon
authors.initials: MB
authors.lastname: Nogueira
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Debur
authors.forename: Luís Felipe Cavalli
authors.initials: LF
authors.lastname: Pessa
authors.forename: Luciane Aparecida
authors.initials: LA
authors.lastname: Pereira
authors.forename: Indianara
authors.initials: I
authors.lastname: Rotta
authors.forename: Gislene Reche de Almeida
authors.initials: GR
authors.lastname: Takahashi
authors.forename: Clyete Santos da
authors.initials: CS
authors.lastname: Silveira
authors.forename: Josianne Maria Reimann
authors.initials: JM
authors.lastname: Araújo
authors.forename: Sonia Mara
authors.initials: SM
authors.lastname: Raboni
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D004770:Enterovirus / Q000235:genetics*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology*; D005260:Female; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000821:virology*; D004854:Herpesvirus 4, Human / Q000235:genetics; D015654:Herpesvirus 6, Human / Q000235:genetics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008587:Meningitis, Viral / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000134:cerebrospinal fluid; D018139:Simplexvirus / Q000235:genetics; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral