title: Epitope mapping of antibodies to VlsE protein of Borrelia burgdorferi in post-Lyme disease syndrome.
abstract: The VlsE lipoprotein of Borrelia burgdorferi elicits a strong immune response during the course of Lyme disease. The present study was aimed at characterization of the epitopes of VlsE targeted by the antibody response in patients with post-Lyme disease syndrome, a condition characterized by persisting symptoms of pain, fatigue, and/or neurocognitive impairment despite antibiotic treatment of B. burgdorferi infection. Epitope mapping was carried out using microarrays that contained synthesized overlapping peptides covering the full sequence of VlsE from B. burgdorferi B31. In addition to the previously characterized IR6 region in the variable domain, specific sequences in the N- and C-terminal invariable domains of VlsE were found to be major B cell epitopes in affected patients. The crystal structure of VlsE indicated that the newly described epitopes form a contiguous region in the surface-exposed membrane-proximal part of the monomeric form of the protein.
authors.affiliation: Department of Neurology and Neuroscience, Weill Cornell Medical College, Cornell University, New York, NY, USA.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Chandra
authors.forename: Norman
authors.initials: N
authors.lastname: Latov
authors.forename: Gary P
authors.initials: GP
authors.lastname: Wormser
authors.forename: Adriana R
authors.initials: AR
authors.lastname: Marques
authors.forename: Armin
authors.initials: A
authors.lastname: Alaedini
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000276:immunology; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D020852:Lyme Neuroborreliosis / Q000276:immunology* / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008958:Models, Molecular; D008969:Molecular Sequence Data; D040081:Protein Array Analysis; D013577:Syndrome
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi