title: Changes in mitochondrial reactive oxygen species synthesis during differentiation of skeletal muscle cells.
abstract: Myogenesis is accompanied by an intensive metabolic remodeling. We investigated the mitochondrial reactive oxygen species (ROS) generation at different levels of skeletal muscle differentiation: in C2C12 myoblasts, in C2C12 myotubes and in adult mouse skeletal muscle. Differentiation was accompanied by an increase in mitochondrial content and respiratory chain activity. The detected ROS production levels correlated with mitochondrial content, being the lowest in the myoblasts. Unlike the adult skeletal muscle, myoblast ROS production was significantly stimulated by the complex I inhibitor rotenone. Our results show that mitochondria are an important ROS source in skeletal muscle cells. The substantial changes in mitochondrial ROS synthesis during skeletal muscle differentiation can be explained by intensive bioenergetic remodeling.
authors.affiliation: Nencki Institute of Experimental Biology, 3 Pasteur St., 02-093 Warsaw, Poland. d.malinska@nencki.gov.pl
authors.forename: Dominika
authors.initials: D
authors.lastname: Malinska
authors.forename: Alexei P
authors.initials: AP
authors.lastname: Kudin
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Bejtka
authors.forename: Wolfram S
authors.initials: WS
authors.lastname: Kunz
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species