title: Influence of charge densities of randomly sulfonated polystyrene surfaces on cell attachment and proliferation.
abstract: Attachment and proliferation of NIH-3T3 fibroblast cells on random polymer surfaces, polystyrene sulfonated acid (PSSAx) with five different degrees of sulfonation (x = 0%, 5%, 10%, 15% and 33%) and on a tissue culture polystyrene (TCPS) surface were studied. The surface properties, wettability and roughness were measured by water-contact angle and atomic force microscopy measurement. The wettability and surface roughness increased with increasing the content of sulfonic acid groups on the surfaces. The number of cells attached on the surface after seeding increased with increasing x and reached to the maximum value on PSSA15. The cell proliferation also increased with increasing x. However, cell proliferation was slow down on PSSA33 in comparison to PSSA10 and PSSA15 surfaces after 48 h culture.
authors.affiliation: Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang-HANARO Joint Center for Biological Interfaces, Sogang University, Seoul 121-742, Korea.
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Khatua
authors.forename: Byeongdo
authors.initials: B
authors.lastname: Kwak
authors.forename: Kwanwoo
authors.initials: K
authors.lastname: Shin
authors.forename: Ju-Myung
authors.initials: JM
authors.lastname: Song
authors.forename: Joon-Seop
authors.initials: JS
authors.lastname: Kim
authors.forename: Jai-Hak
authors.initials: JH
authors.lastname: Choi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D051379:Mice; D041681:NIH 3T3 Cells; D011137:Polystyrenes / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: D011137:Polystyrenes; D013451:Sulfonic Acids