title: Due to intravascular multiple sequential scattering, Diffuse Correlation Spectroscopy of tissue primarily measures relative red blood cell motion within vessels.
abstract: We suggest that Diffuse Correlation Spectroscopy (DCS) measurements of tissue blood flow primarily probe relative red blood cell (RBC) motion, due to the occurrence of multiple sequential scattering events within blood vessels. The magnitude of RBC shear-induced diffusion is known to correlate with flow velocity, explaining previous reports of linear scaling of the DCS "blood flow index" with tissue perfusion despite the observed diffusion-like auto-correlation decay. Further, by modeling RBC mean square displacement using a formulation that captures the transition from ballistic to diffusive motion, we improve the fit to experimental data and recover effective diffusion coefficients and velocity de-correlation time scales in the range expected from previous blood rheology studies.
authors.affiliation: Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA.
authors.forename: Stefan A
authors.initials: SA
authors.lastname: Carp
authors.forename: Nadàege
authors.initials: N
authors.lastname: Roche-Labarbe
authors.forename: Maria-Angela
authors.initials: MA
authors.lastname: Franceschini
authors.forename: Vivek J
authors.initials: VJ
authors.lastname: Srinivasan
authors.forename: Sava
authors.initials: S
authors.lastname: Sakadžić
authors.forename: David A
authors.initials: DA
authors.lastname: Boas