title: Castration-induced changes in mouse epididymal white adipose tissue.
abstract: We analyzed the effects of castration on epididymal white adipose tissue (WAT) in C57BL/6J mice which were fed a regular or high-fat diet. Fourteen days following surgical castration profound effects on WAT tissue such as reductions in WAT wet weight and WAT/body weight ratio, induction of lipolysis and morphologic changes characterized by smaller adipocytes, and increased stromal cell compartment were documented in both dietary groups. Castrated animals had decreased serum leptin levels independent of diet but diet-dependent decreases in serum adiponectin and resistin. The castrated high-fat group had dramatically lower serum triglyceride levels. Immunohistochemical analysis revealed higher staining for smooth muscle actin, macrophage marker Mac-3, and Cxcl5 in the castrated than in the control mice in both dietary groups. We also detected increased fatty-acid synthase expression in the stromal compartment of WAT in the regular-diet group. Castration also reduces the expression of androgen receptor in WAT in the regular-diet group. We conclude that castration reduces tissue mass and affects biologic function of WAT in mice.
authors.affiliation: Department of Genitourinary Medical Oncology - Research, The University of Texas MD Anderson Cancer Center, Houston, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Floryk
authors.forename: Shinji
authors.initials: S
authors.lastname: Kurosaka
authors.forename: Ryuta
authors.initials: R
authors.lastname: Tanimoto
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Alexei
authors.initials: A
authors.lastname: Goltsov
authors.forename: Sanghee
authors.initials: S
authors.lastname: Park
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Thompson
mesh_terms: D054392:Adipokines / Q000097:blood; D052436:Adipose Tissue, White / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D002369:Castration; D059305:Diet, High-Fat; D004822:Epididymis / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D008066:Lipolysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011944:Receptors, Androgen / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D054392:Adipokines; D001786:Blood Glucose; D011944:Receptors, Androgen; D014280:Triglycerides