title: PEG and mPEG-anthracene induce DNA condensation and particle formation.
abstract: In this study, we investigated the binding of DNA with poly(ethylene glycol) (PEG) of different sizes and compositions such as PEG 3350, PEG 6000, and mPEG-anthracene in aqueous solution at physiological conditions. The effects of size and composition on DNA aggregation and condensation as well as conformation were determined using Fourier transform infrared (FTIR), UV-visible, CD, fluorescence spectroscopic methods and atomic force microscopy (AFM). Structural analysis showed moderate complex formation for PEG 3350 and PEG 6000 and weaker interaction for mPE-anthracene-DNA adducts with both hydrophilic and hydrophobic contacts. The order of ± stability of the complexes formed is K(PEG 6000) = 1.5 (±0.4) × 10(4) M(-1) > K(PEG 3350) = 7.9 (±1) × 10(3) M(-1) > K(m(PEG-anthracene))= 3.6 (±0.8) × 10(3) M(-1) with nearly 1 bound PEG molecule per DNA. No B-DNA conformational changes were observed, while DNA condensation and particle formation occurred at high PEG concentration.
authors.affiliation: Department of Chemistry-Biology, University of Québec at Trois-Rivières, C.P. 500, Trois-Rivières (Québec), Canada G9A 5H7.
authors.forename: E
authors.initials: E
authors.lastname: Froehlich
authors.forename: J S
authors.initials: JS
authors.lastname: Mandeville
authors.forename: D
authors.initials: D
authors.lastname: Arnold
authors.forename: L
authors.initials: L
authors.lastname: Kreplak
authors.forename: H A
authors.initials: HA
authors.lastname: Tajmir-Riahi
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000737:chemistry*; D001665:Binding Sites; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000873:Anthracenes; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D004247:DNA; C427203:calf thymus DNA; C034020:anthracene