title: Detection of beta-amyloid (1-42) on protein array based on electrical detection technique using scanning tunneling microscopy.
abstract: In this study an immuno-array for Abeta42 based on scanning tunneling microscopy (STM) was developed using conjugated gold nanoparticle (Au-NP) and antibody (Ab) complex. Fragmented monoclonal Ab against Abeta42 was allowed to immobilize on the Au-dot arrays followed by its target protein Abeta42 and Au NP and Ab complex. The surface structure of Au-NP and Ab complex on Au-dots was investigated with Atomic Force Microscopy and the current profile of fabricated immunosensing element was investigated with STM. The power spectrum derived from the current profile was found to be increasing with higher concentrations of Abeta42 having a detection limit of 100 fg/ml. The proposed technique can be a promising method to construct the highly sensitive and efficient protein chip of immunosensors arrays.
authors.affiliation: Interdisciplinary Program of Integrated Biotechnology, Sogang University Seoul 121-742, Korea.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
authors.forename: A T M Kamrul
authors.initials: AT
authors.lastname: Islam
authors.forename: Jin-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D010446:Peptide Fragments / Q000032:analysis*; D040081:Protein Array Analysis
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D006046:Gold