title: The additive effect of mesenchymal stem cells and bone morphogenetic protein 2 on γ-irradiated bone marrow in mice.
abstract: Irradiation from γ-rays can cause severe damage to bone marrow and hematopoietic tissues. Presently, the most effective method available to treat severe hematopoietic injury is a bone marrow transplant (BMT). Allogeneic BMT is a difficult technique to perform due to the differences in human leukocyte antigen proteins between the donor and recipient, with acute graft-versus-host disease being a major complication of the technique. This limits the widespread applicability of allogeneic BMT. To develop a novel treatment for acute hematopoietic damage, we transplanted bone marrow derived mesenchymal stem cells (MSCs) into recipient mice and treated them with recombinant human bone morphogenetic protein 2 (rhBMP2) to investigate whether MSCs and rhBMP2 could additively promote the restoration of hematopoietic function. MSCs are vital components of the hematopoietic microenvironment that supports hematopoiesis, and bone morphogenic protein is a key factor in hematopoiesis. The 30-day survival rate as well as the numbers of nucleated cells, bone marrow colony-forming unit-granulocyte macrophages, spleen colony-forming units and peripheral blood cells were enumerated. The results showed that, after γ-irradiation and transplantation, MSCs and rhBMP2 additively promoted and improved hematopoietic restoration and function in vivo and in vitro. This additive effect of MSCs and rhBMP2 may one day provide a novel means of treating acute hematopoietic damage.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shuibing
authors.initials: S
authors.lastname: Liu
authors.forename: Peizhen
authors.initials: P
authors.lastname: Hu
authors.forename: Ying
authors.initials: Y
authors.lastname: Hou
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Xubo
authors.initials: X
authors.lastname: Li
authors.forename: Qiong
authors.initials: Q
authors.lastname: Tian
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002452:Cell Count; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D055014:Granulocyte-Macrophage Progenitor Cells / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D016019:Survival Analysis
chemical_list: D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2