title: Structural and in vitro cytotoxicity studies on 1H-benzimidazol-2-ylmethyl-N-phenyl amine and its Pd(II) and Pt(II) complexes.
abstract: [MLCl(2)]·zH(2)O (L = (1H-benzimidazol-2-ylmethyl)-N-phenyl amine; M = Pd, z = 0; M = Pt, z = 1) and [PdL(OH(2))(2)]·2X·zH(2)O (X = Br, I, NO(3), z = 0; X = SCN, z = 1) complexes were synthesized as potential anticancer compounds and characterized by elemental analysis, spectral and thermal methods. FT-IR and (1)H NMR studies revealed that the benzimidazole L is coordinated to the metal ions via the pyridine-type nitrogen (N(py)) of the benzimidazole ring and secondary amino group (NH(sec)). Quantum mechanical calculations of energies, geometries, vibrational wavenumbers, and (1)H NMR of the benzimidazole L and its complexes were carried out by density functional theory using B3LYP functional combined with 6-31G(d) and LANL2DZ basis sets. Natural bond orbitals (NBOs) and frontier molecular orbitals were performed at B3LYP/LANL2DZ level of theory. The synthesized ligand, in comparison to its metal complexes was screened for its antibacterial activity. The benzimidazole L is more toxic against the bacterium Staphylococcus aureus (MIC = 58 μg/mL) than the standard tetracycline (MIC = 82 μg/mL). The complexes showed cytotoxicity against breast cancer, Colon Carcinoma, and human heptacellular Carcinoma cells. The platinum complex (6) displays cytotoxicity (IC(50) = 12.4 μM) against breast cancer compared with that reported for cis-platin 9.91 μM.
authors.affiliation: Chemistry Department, Faculty of Science, Cairo University, Gamaa Street, Giza 12613, Egypt.
authors.forename: Nour T
authors.initials: NT
authors.lastname: Abdel Ghani
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Mansour
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000494:pharmacology; D001562:Benzimidazoles / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology*; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D056945:Hep G2 Cells; D006801:Humans; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D008958:Models, Molecular; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000494:pharmacology; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D018675:Toxicity Tests; D014407:Tumor Cells, Cultured
chemical_list: C569550:1H-benzimidazol-2-ylmethyl-N-phenylamine; D000814:Aniline Compounds; D001562:Benzimidazoles; D056831:Coordination Complexes; D003603:Cytotoxins; D009942:Organometallic Compounds; D010984:Platinum; D010165:Palladium