title: [Association of combined polymorphisms in MIP-1α and ApoE genes with the susceptibility of inflammatory bowel disease].
abstract: OBJECTIVE
  To investigate whether the macrophage inflammatory protein 1 alpha (MIP-1α) and apolipoprotein E (ApoE) gene polymorphisms, either alone or in combination, affect the susceptibility to inflammatory bowel disease (IBD).
  
  
  METHODS
  Genomic DNA of IBD patients with Crohn's disease (CD, n = 41) and with ulcerative colitis (UC, n = 142) and healthy controls (n = 160) was extracted and genotyped for the MIP-1α and ApoE gene polymorphisms by restriction fragment length polymorphism assay.
  
  
  RESULTS
  MIP-1α -906(TA)(6)/(TA)(6) homozygotes had a significantly elevated risk of UC (OR = 1.909, 95%CI = 1.204 - 3.028). The carriers of APOE4ε4 were at a significantly higher risk for UC with OR of 2.379 (95% CI = 1.451 - 3.896). And a combination of these two loci, MIP-1α -906(TA)(6)/(TA)(6)/APOE4ε4 were strongly associated with a higher risk of UC (OR = 3.288; 95%CI = 1.777 - 6.084).
  
  
  CONCLUSIONS
  The polymorphisms of MIP-1α -906 (TA)(6)/(TA)(6) and ApoE are probably independent genetic risk factors for UC. And the coexistence of both may exert an additive effect on the UC risks.
authors.affiliation: School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, China.
authors.forename: Wan-Dong
authors.initials: WD
authors.lastname: Liang
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Yan
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Ke-Shen
authors.initials: KS
authors.lastname: Li
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Yun-Tian
authors.initials: YT
authors.lastname: Bi
mesh_terms: D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics*; D016022:Case-Control Studies; D054405:Chemokine CCL3 / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000235:genetics*; D003424:Crohn Disease / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D055815:Young Adult
chemical_list: D001057:Apolipoproteins E; C515429:CCL3 protein, human; D054405:Chemokine CCL3