title: Development of a New Tool for 3D Modeling for Regenerative Medicine.
abstract: The effectiveness of therapeutic treatment based on regenerative medicine for degenerative diseases (i.e., neurodegenerative or cardiac diseases) requires tools allowing the visualization and analysis of the three-dimensional (3D) distribution of target drugs within the tissue. Here, we present a new computational procedure able to overcome the limitations of visual analysis emerging by the examination of a molecular signal within images of serial tissue/organ sections by using the conventional techniques. Together with the 3D anatomical reconstitution of the tissue/organ, our framework allows the detection of signals of different origins (e.g., marked generic molecules, colorimetric, or fluorimetric substrates for enzymes; microRNA; recombinant protein). Remarkably, the application does not require the employment of specific tracking reagents for the imaging analysis. We report two different representative applications: the first shows the reconstruction of a 3D model of mouse brain with the analysis of the distribution of the β-Galactosidase, the second shows the reconstruction of a 3D mouse heart with the measurement of the cardiac volume.
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Sezione di Biochimica e Biologia Molecolare, Università degli Studi di Perugia, Via del Giochetto, 06126 Perugia, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Mattoli
authors.forename: Roberto
authors.initials: R
authors.lastname: Tiribuzi
authors.forename: Francesco
authors.initials: F
authors.lastname: D'Angelo
authors.forename: Ilaria
authors.initials: I
authors.lastname: di Girolamo
authors.forename: Mattia
authors.initials: M
authors.lastname: Quattrocelli
authors.forename: Simona
authors.initials: S
authors.lastname: Montesano
authors.forename: Lucia
authors.initials: L
authors.lastname: Crispoltoni
authors.forename: Vasileios
authors.initials: V
authors.lastname: Oikonomou
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: Peggy
authors.initials: P
authors.lastname: Marconi
authors.forename: Antonio
authors.initials: A
authors.lastname: Orlacchio
authors.forename: Maurilio
authors.initials: M
authors.lastname: Sampaolesi
authors.forename: Sabata
authors.initials: S
authors.lastname: Martino
authors.forename: Aldo
authors.initials: A
authors.lastname: Orlacchio