title: Multiplexed, ligation-dependent probe amplification for rapid and inexpensive HLA-DQB1 allelotyping.
abstract: Many effective options exist to accurately type DNA for human leukocyte antigen (HLA) alleles. However, most of the existing methods are excessively costly in terms of overall monetary costs, DNA requirements, and proprietary software. We present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at two digits, with even greater specificity for the HLA-DQB1*06 allele family, by using the multiplexed ligation-dependent probe amplification technology. This assay provides more specific allele data than genome-wide analysis and is more affordable than sequencing, making it a useful intermediate for researchers seeking to accurately allelotype human DNA samples.
authors.affiliation: Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley, Berkeley, CA, USA. kipp@berkeley.edu
authors.forename: N K
authors.initials: NK
authors.lastname: Akers
authors.forename: J D
authors.initials: JD
authors.lastname: Curry
authors.forename: M T
authors.initials: MT
authors.lastname: Smith
authors.forename: P M
authors.initials: PM
authors.lastname: Bracci
authors.forename: C F
authors.initials: CF
authors.lastname: Skibola
mesh_terms: D000483:Alleles; D002460:Cell Line; D005260:Female; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006579:Heterozygote; D006650:Histocompatibility Testing / Q000191:economics / Q000379:methods*; D006801:Humans; D022521:Ligase Chain Reaction / Q000191:economics / Q000379:methods*; D008297:Male; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D015345:Oligonucleotide Probes