title: Prolactin increases survival and migration of ovarian cancer cells: importance of prolactin receptor type and therapeutic potential of S179D and G129R receptor antagonists.
abstract: Variably-spliced prolactin receptors (PRLRs) and PRL are expressed by the ovarian cancer cell lines, TOV-112D, OV-90 and TOV-21G. Incubation in the PRLR antagonists, G129R- or S179D-PRL, or anti-PRL reduced cell number, indicating a functional autocrine PRL growth loop. Added PRL promoted, and the antagonists decreased, cell migration. When cells were stressed, added PRL decreased apoptosis and increased survival, and the antagonists had the opposite effect. Cells expressing higher long:short PRLR ratios had increased growth, survival and migration in response to PRL. Results suggest that PRLR antagonists may be therapeutically beneficial in ovarian cancer.
authors.affiliation: Division of Biomedical Sciences, University of California, Riverside, CA 92521-0121, USA.
authors.forename: Dunyong
authors.initials: D
authors.lastname: Tan
authors.forename: Kuanhui E
authors.initials: KE
authors.lastname: Chen
authors.forename: Teresa
authors.initials: T
authors.lastname: Khoo
authors.forename: Ameae M
authors.initials: AM
authors.lastname: Walker
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018613:Microscopy, Confocal; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011388:Prolactin / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D020033:Protein Isoforms / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011981:Receptors, Prolactin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020033:Protein Isoforms; D011981:Receptors, Prolactin; D011994:Recombinant Proteins; C402398:prolactin, Arg(129)-; C422171:prolactin, Asp(179)-; D011388:Prolactin