title: Inhibitory effect of grapefruit juice on the genotoxicity induced by hydrogen peroxide in human lymphocytes.
abstract: By means of the comet assay we demonstrated a strong effect by hydrogen peroxide (HP) and no damage by grapefruit juice (GJ) in human lymphocytes. Cells exposed to HP and treated with three concentrations of GJ (10-90 min) showed an increase of DNA damage by HP over the control level, and a decrease of such damage by GJ. With the comet assay plus formamidopyrimidine-DNA-glycosylase we found the strongest increase of DNA damage by HP over the control level, and the strongest reduction of such damage by GJ. By applying the comet/FISH method we determined 98% of the p53 gene signals in the comet head of control cells along the experiment (10-90 min), in contrast with about 90% signals in the comet tail of cells exposed to HP. Cells treated with both agents showed a significant, concentration/time dependent return of p53 signals to the head, suggesting enhancement of the gene repair. Finally, with the annexin V assay we found an increase in apoptosis and necrosis by HP, and no effect by GJ; when GJ was added to HP treated cells no modification was observed in regard to apoptosis, although a decrease of necrosis was observed.
authors.affiliation: Instituto Nacional de Perinatología, SSA, Montes Urales 800, Lomas Virreyes, 11000 México DF, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Razo-Aguilera
authors.forename: R
authors.initials: R
authors.lastname: Baez-Reyes
authors.forename: I
authors.initials: I
authors.lastname: Alvarez-González
authors.forename: R
authors.initials: R
authors.lastname: Paniagua-Pérez
authors.forename: E
authors.initials: E
authors.lastname: Madrigal-Bujaidar
mesh_terms: D000328:Adult; D017304:Annexin A5; D001628:Beverages; D032083:Citrus paradisi; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects*; D055815:Young Adult
chemical_list: D017304:Annexin A5; D006861:Hydrogen Peroxide