title: Association of bla(DHA-1) and qnrB genes carried by broad-host-range plasmids among isolates of Enterobacteriaceae at a Spanish hospital.
abstract: A collection of 30 DHA-1-Enterobacteriaceae producers was examined for the presence of qnr genes. PCR-based replicon typing, plasmid profile and Southern hybridisation analyses revealed that all isolates co-harboured bla(DHA-1) and qnrB genes on the same plasmid. All but one of these plasmids belonged to the L/M group. Genetic organization analyses of a randomly selected isolate revealed the co-localization of both genes on an IS26-composite transposon. As plasmids carrying both genes seem to have a high prevalence and a worldwide distribution, care should be taken when quinolones are used to treat infections caused by DHA-1 producers.
authors.affiliation: Departament de Genètica i de Microbiologia, Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Institut d'Investigacions Biomèdiques Sant Pau, Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Mata
authors.forename: E
authors.initials: E
authors.lastname: Miró
authors.forename: M
authors.initials: M
authors.lastname: Toleman
authors.forename: M A
authors.initials: MA
authors.lastname: Rivera
authors.forename: T R
authors.initials: TR
authors.lastname: Walsh
authors.forename: F
authors.initials: F
authors.lastname: Navarro
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D005798:Genes, Bacterial / Q000187:drug effects; D006761:Hospitals; D058507:Host Specificity; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D001618:beta-Lactamases