title: The immune response of turbot, Scophthalmus maximus (L.), skin to high water temperature.
abstract: The immune responses of mucus from the skin of turbot, Scophthalmus maximus, were studied in relation to changing water temperature. Groups of fish were exposed to a programmed increase of 3 °C per 48 h, until the experimental water temperatures of 16, 20, 23, 25, 27 or 28 °C were reached. After 48 h at the relevant temperature, the expressions of immune-related factors were determined, including immunoglobulin M (IgM), IL-1β, hepcidin, transferrin, lysozyme, acid/alkaline phosphatase and superoxide dismutase using RT-PCR and spectrophotometric methods. Significant changes in mucus immunity were observed, which paralleled with those previously reported for serum in other fish species. Hence, it is suggested that the serum and mucus immune system of turbot have a similar regulatory system. This information could be useful in better understanding the role of the mucus as a component of the innate immune system.
authors.affiliation: Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao, China.
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Huang
authors.forename: A-J
authors.initials: AJ
authors.lastname: Ma
authors.forename: X-A
authors.initials: XA
authors.lastname: Wang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism; D004848:Epithelium / Q000276:immunology; D005412:Flatfishes / Q000276:immunology* / Q000378:metabolism; D064451:Hepcidins; D007113:Immunity, Innate; D053583:Interleukin-1beta / Q000378:metabolism; D009093:Mucus / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012712:Serum Globulins / Q000378:metabolism; D012867:Skin / Q000276:immunology*; D013696:Temperature
chemical_list: D023181:Antimicrobial Cationic Peptides; D064451:Hepcidins; D053583:Interleukin-1beta; D012712:Serum Globulins