title: Multivariate optimization of cloud point extraction procedure for zinc determination in aqueous extracts of medicinal plants by flame atomic absorption spectrometry.
abstract: Cloud point extraction method has been developed for preconcentration of trace quantities of zinc (Zn) in aqueous extract of medicinal plants and blood samples of liver cancer patients using flame atomic absorption spectrometry. The Zn in aqueous extracts of medicinal plants (MPs) was complexed with 2-methyl-8-hydroxyquinoline (quinaldine) and 1-(2-pyridylazo)-2-naphthol (PAN) separately and entrapped in a non-ionic surfactant Triton X-114. After centrifugation, the surfactant-rich phase was diluted with 0.25mL acidic ethyl alcohol. The multivariate strategy was applied to estimate the optimum values of experimental variables (pH, time temperature, ligands and surfactant concentrations). Interactions between analytical factors and their optimal levels were investigated by two level factorial designs. Student's t-test on the results of factorial design with 16 runs for Zn extraction, demonstrated that the factors, ligands concentrations, pH and temperature were statistically significant. The accuracy was assessed by analysis of certified reference materials, namely, BCR 101 (spruce needles), Clincheck control-lyophilized human whole blood. Enhancement factor of 30 and 26 were achieved for the preconcentration of Zn by 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2), respectively. The relative standard deviation for six replicate determinations of Zn at 10μg/L level using 2-methyl-8-hydroxyquinoline (L1) and 1-(2-pyridylazo)-2-naphthol (L2) were <4% and >5%, respectively.
authors.affiliation: National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan. nidafatima6@gmail.com
authors.forename: N F
authors.initials: NF
authors.lastname: Kolachi
authors.forename: T G
authors.initials: TG
authors.lastname: Kazi
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: S K
authors.initials: SK
authors.lastname: Wadhwa
authors.forename: J A
authors.initials: JA
authors.lastname: Baig
authors.forename: H I
authors.initials: HI
authors.lastname: Afridi
authors.forename: A Q
authors.initials: AQ
authors.lastname: Shah
authors.forename: F
authors.initials: F
authors.lastname: Shah
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008113:Liver Neoplasms / Q000097:blood*; D008875:Middle Aged; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D015032:Zinc / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts; D015032:Zinc