title: Molecular and biological characterization of a new Brazilian begomovirus, euphorbia yellow mosaic virus (EuYMV), infecting Euphorbia heterophylla plants.
abstract: To date, no begomovirus has been fully characterized from Euphorbia heterophylla, a widely distributed weed, in Brazil. Here, we show the occurrence of a new begomovirus on E. heterophylla plants showing bright yellow mosaic. The bipartite viral genome was cloned from 10 samples, and all clones are almost identical to each other (95.6-98.8% nucleotide sequence identity). The DNA-A sequences shared a maximum nucleotide sequence identity of 87.3% with euphorbia mosaic Peru virus (EuMPV) and thus were classified as belonging to a novel begomovirus species, tentatively named Euphorbia yellow mosaic virus (EuYMV). The EuYMV DNA-B sequences share a maximum nucleotide sequence identity of 56.2% with a euphorbia mosaic virus (EuMV) isolate from Mexico. Phylogenetic analysis demonstrated that this new virus belongs to a different lineage than EuMV isolates from Central America.
authors.affiliation: Embrapa Vegetables, Brasília, DF 70359-970, Brazil. fernanda@cnph.embrapa.br
authors.forename: Fernanda R
authors.initials: FR
authors.lastname: Fernandes
authors.forename: Leonardo C
authors.initials: LC
authors.lastname: Albuquerque
authors.forename: Cristiane L
authors.initials: CL
authors.lastname: de Oliveira
authors.forename: Andréa R R
authors.initials: AR
authors.lastname: Cruz
authors.forename: Wesley B
authors.initials: WB
authors.lastname: da Rocha
authors.forename: Talita G
authors.initials: TG
authors.lastname: Pereira
authors.forename: Fernanda Y B
authors.initials: FY
authors.lastname: Naito
authors.forename: Natália de M
authors.initials: Nde M
authors.lastname: Dias
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
authors.forename: Josias C
authors.initials: JC
authors.lastname: Faria
authors.forename: Francisco M
authors.initials: FM
authors.lastname: Zerbini
authors.forename: Francisco J L
authors.initials: FJ
authors.lastname: Aragão
authors.forename: Alice K
authors.initials: AK
authors.lastname: Inoue-Nagata
mesh_terms: D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001938:Brazil; D028481:Euphorbia / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*