title: [Identification of Haemaphysalis longicornis and Rhipicephalus microplus].
abstract: OBJECTIVE
  To establish a method combined morphology and molecular marker for identifying Haemaphysalis longicornis and Rhipicephalus microplus.
  
  
  METHODS
  Ticks were collected from domestic animals and wild environment in epidemic area of Hubei and Henan provinces where cases of fever with thrombocytopenia syndrome were prevalent. We classified the ticks by morphology characteristics before 12S rDNA of ticks were amplified by PCR and subsequently sequenced. Phylogenetic tree was constructed by PAUP4.0.
  
  
  RESULTS
  The ticks belonged to Haemaphysalis longicornis and Rhipicephalus microplus through observation and analysed by the morphological characteristics of the ticks. 12S rDNA was cloned and sequenced while data confirmed the morphological identification of the results.
  
  
  CONCLUSIONS
  The method based on morphology that combined with molecular marker seemed a good method for the identification of ticks.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Xin-cheng
authors.initials: XC
authors.lastname: Qin
authors.forename: Jun-hua
authors.initials: JH
authors.lastname: Tian
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Qiang-zheng
authors.initials: QZ
authors.lastname: Sun
authors.forename: Dong
authors.initials: D
authors.lastname: Jin
authors.forename: Dun-jin
authors.initials: DJ
authors.lastname: Zhou
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Xu
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal; D026863:Ixodidae / Q000033:anatomy & histology* / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D048494:Rhipicephalus / Q000033:anatomy & histology* / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S