title: Glycolipid-cholesterol monolayers: towards a better understanding of the interaction between the membrane components.
abstract: In this work, the interaction between a synthetic analog of archaeal lipids and cholesterol was studied using Langmuir technique. The lipid, β-Mal(3)O(C(16+4))(2), contained phytanyl chains attached via two ether bonds to the sn-2 carbon of the glycerol backbone. The preliminary studies showed that monolayers formed with the pure lipid have a liquid-like character; here, a hypothesis that admixing cholesterol to β-Mal(3)O(C(16+4))(2) could confer a higher rigidity on the films was tested. To check this proposal, two-dimensional miscibility of cholesterol and β-Mal(3)O(C(16+4))(2) in monomolecular films was studied using surface pressure and surface potential measurements, as well as Brewster angle microscopy and polarization-modulation infrared reflection absorption spectroscopy. The stability of the monomolecular films was evaluated based on thermodynamics of mixing of cholesterol and β-Mal(3)O(C(16+4))(2). Atomic level information concerning the orientation of molecules and the degree of hydration of polar headgroups was obtained from molecular dynamics simulations.
authors.affiliation: Department of Physical Chemistry and Electrochemistry, Jagiellonian University, Krakow, Poland. bkorch@chemia.uj.edu.pl
authors.forename: Beata
authors.initials: B
authors.lastname: Korchowiec
authors.forename: Jacek
authors.initials: J
authors.lastname: Korchowiec
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Hato
authors.forename: Ewa
authors.initials: E
authors.lastname: Rogalska
mesh_terms: D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry*; D008969:Molecular Sequence Data; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D006017:Glycolipids; D002784:Cholesterol