title: Chirality in planar cell shape contributes to left-right asymmetric epithelial morphogenesis.
abstract: Some organs in animals display left-right (LR) asymmetry. To better understand LR asymmetric morphogenesis in Drosophila, we studied LR directional rotation of the hindgut epithelial tube. Hindgut epithelial cells adopt a LR asymmetric (chiral) cell shape within their plane, and we refer to this cell behavior as planar cell-shape chirality (PCC). Drosophila E-cadherin (DE-Cad) is distributed to cell boundaries with LR asymmetry, which is responsible for the PCC formation. Myosin ID switches the LR polarity found in PCC and in DE-Cad distribution, which coincides with the direction of rotation. An in silico simulation showed that PCC is sufficient to induce the directional rotation of this tissue. Thus, the intrinsic chirality of epithelial cells in vivo is an underlying mechanism for LR asymmetric tissue morphogenesis.
authors.affiliation: Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Taniguchi
authors.forename: Reo
authors.initials: R
authors.lastname: Maeda
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ando
authors.forename: Takashi
authors.initials: T
authors.lastname: Okumura
authors.forename: Naotaka
authors.initials: N
authors.lastname: Nakazawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Hatori
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Nakamura
authors.forename: Shunya
authors.initials: S
authors.lastname: Hozumi
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fujiwara
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsuno
mesh_terms: D022005:Adherens Junctions; D000818:Animals; D019521:Body Patterning; D015820:Cadherins / Q000378:metabolism*; D016764:Cell Polarity; D048430:Cell Shape; D003198:Computer Simulation; D004330:Drosophila / Q000166:cytology / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology*; D007422:Intestines / Q000166:cytology / Q000196:embryology; D008954:Models, Biological; D009024:Morphogenesis; D024461:Myosin Type I / Q000235:genetics / Q000378:metabolism*; D012399:Rotation
chemical_list: D015820:Cadherins; D029721:Drosophila Proteins; C513226:Myo31DF protein, Drosophila; C501889:shg protein, Drosophila; D024461:Myosin Type I