title: Ultra high performance liquid chromatographic-tandem mass spectrometric multi-analyte procedure for target screening and quantification in human blood plasma: validation and application for 31 neuroleptics, 28 benzodiazepines, and Z-drugs.
abstract: For fast and reliable screening, identification, and quantification of as many analytes as possible, multi-analyte approaches are very useful in clinical and forensic toxicology. Using ultra high performance liquid chromatography-tandem mass spectrometry, such an approach has been developed for blood plasma analysis after simple liquid-liquid extraction. In the present paper, validation and application is described for 31 neuroleptics, 28 benzodiazepines, and Z-drugs (zaleplone, zolpidem, and zopiclone). The validation parameters included recovery, matrix effects, process efficiency, ion suppression/enhancement of co-eluting analytes, selectivity, crosstalk, accuracy and precision, stabilities, and limits of quantification and detection. The results showed that the approach was selective, sensitive, accurate, and precise for 24 neuroleptics and 21 benzodiazepines and Z-drugs. The remaining analytes were unstable and/or too low dosed. Cost- and time-saving one-point calibration was applicable only for half of the analytes. The applicability was successfully shown for most of the drugs by analyzing authentic plasma samples and external quality control samples.
authors.affiliation: Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology, Saarland University, Homburg, Saar, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Remane
authors.forename: Markus R
authors.initials: MR
authors.lastname: Meyer
authors.forename: Dirk K
authors.initials: DK
authors.lastname: Wissenbach
authors.forename: Hans H
authors.initials: HH
authors.lastname: Maurer
mesh_terms: D000081:Acetamides / Q000097:blood; D014150:Antipsychotic Agents / Q000097:blood*; D053961:Azabicyclo Compounds / Q000097:blood; D001569:Benzodiazepines / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D057230:Limit of Detection; D010879:Piperazines / Q000097:blood; D011725:Pyridines / Q000097:blood; D011743:Pyrimidines / Q000097:blood; D053719:Tandem Mass Spectrometry; D000077334:Zolpidem
chemical_list: D000081:Acetamides; D014150:Antipsychotic Agents; D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; D011725:Pyridines; D011743:Pyrimidines; C515050:zopiclone; D001569:Benzodiazepines; D000077334:Zolpidem; C085665:zaleplon