title: Biomimetic approach to perforation repair using dental pulp stem cells and dentin matrix protein 1.
abstract: BACKGROUND
  Dentin regeneration could be an ideal treatment option to restore tissue function. This study was conducted to evaluate the ability of dental pulp stem cells (DPSCs) and dentin matrix protein 1 (DMP1) impregnated within a collagen scaffold to regenerate dentin.
  
  
  METHODS
  Simulated perforations were created in 18 dentin wafers made from freshly extracted human molars. Six groups were established. They were (1) empty wafers, (2) mineral trioxide aggregate, (3) collagen scaffold, (4) scaffold with DMP1, (5) scaffold with DPSCs, and (6) scaffold with DPSCs and DMP1. One sample was placed subcutaneously in each mouse with three mice in each group. After 12 weeks, the samples were subjected to radiographic, histological, and immunohistochemical evaluations.
  
  
  RESULTS
  DPSCs impregnated within a collagen scaffold differentiated into odontoblast-like cells forming a highly cellular, vascular, and mineralized matrix in the presence of DMP1.
  
  
  CONCLUSIONS
  A triad consisting of DPSCs, DMP1, and a collagen scaffold promotes dentin regeneration in a simulated perforation repair model.
authors.affiliation: Department of Endodontics, University of Illinois at Chicago, Chicago, Illinois, USA.
authors.forename: Rajaa
authors.initials: R
authors.lastname: Alsanea
authors.forename: Sriram
authors.initials: S
authors.lastname: Ravindran
authors.forename: Mohamed I
authors.initials: MI
authors.lastname: Fayad
authors.forename: Bradford R
authors.initials: BR
authors.lastname: Johnson
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Wenckus
authors.forename: Jianjun
authors.initials: J
authors.lastname: Hao
authors.forename: Anne
authors.initials: A
authors.lastname: George
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000502:physiology*; D017607:Aluminum Compounds / Q000627:therapeutic use; D000818:Animals; D032701:Biomimetics; D017610:Calcium Compounds / Q000627:therapeutic use; D002454:Cell Differentiation; D003094:Collagen; D003782:Dental Pulp / Q000166:cytology*; D003786:Dental Pulp Cavity / Q000293:injuries*; D003804:Dentin / Q000293:injuries; D003809:Dentin, Secondary / Q000378:metabolism*; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000000981:diagnostic imaging; D016326:Extracellular Matrix Proteins / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D051379:Mice; D009804:Odontoblasts / Q000502:physiology; D010087:Oxides / Q000627:therapeutic use; D010750:Phosphoproteins / Q000494:pharmacology / Q000502:physiology*; D019252:Radiography, Dental, Digital; D011994:Recombinant Proteins / Q000494:pharmacology; D012038:Regeneration; D012387:Root Canal Filling Materials / Q000627:therapeutic use; D017640:Silicates / Q000627:therapeutic use; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; C513309:DMP1 protein, human; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D010087:Oxides; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D003094:Collagen