title: [Pathway analysis of the differential expression genes of oligonucleotide microarray of airway allergic diseases using GenMAPP].
abstract: OBJECTIVE
  To detected the mechanism of allergic rhinitis associated with asthma with bioinformatics methods.
  
  
  METHODS
  GenMAPP software was used to analyze the expression profile of nasal mucosa of seasonal allergic rhinitis(SAR) and SAR associated with asthma of oligonucleotide microarray (Affymetrix HG-U133-plus2). One the first step,of differentially expressed genes screening were done, then differential gene database retrieval was established, at last pathway analysis was performed.
  
  
  RESULTS
  689 genes out of 47 000 analyzed transcripts of nasal mucosa of SAR associated with asthma were differentially expressed at least 4-fold, in which 233 genes were up regulated and 456 genes were down regulated. These differential expression genes participate in 69 bio-pathways, in which the interaction pathway between cytokine and cytokine receptor was most. Chemotactic factor CXCL12 and its receptor CXCR4 expressed in SAR associated with asthma patients were up-regulated predominantly, compared with that in SAR patients.
  
  
  CONCLUSIONS
  Multiple pathways were involved in the development of SAR and SAR complicated with asthma. The CXCL12/CXCR4 axis might play a main role in the allergic airway diseases.
authors.affiliation: Department of Otolaryngology, the Second Hospital of Shanxi Medical University, Shanxi, 030001, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Xue
authors.forename: Changqing
authors.initials: C
authors.lastname: Zhao
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D001249:Asthma / Q000175:diagnosis / Q000235:genetics*; D054377:Chemokine CXCL12 / Q000378:metabolism; D019295:Computational Biology; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019718:Receptors, CXCR4 / Q000378:metabolism; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis / Q000235:genetics*; D012984:Software
chemical_list: C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4