title: [Polyclonal antibodies against human cell-surface antigen CD34].
abstract: An efficient and inexpensive laboratory approach for the generation and the purification of polyclonal antibodies to human antigen CD34 was developed. It was shown that cloned refolded and purified from Escherichia coli recombinant extracellular fragment of CD34 antigen retained immunogenic determinants of cell-surface expressed CD34. Immunization of mice with unglycosylated truncated recombinant protein elicit polyclonal antibodies specific for the native human antigen CD34. The antibodies generated are applicable for phenotyping of CD34+ cells using immunocytochemistry and flow cytometry assays.
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Nikolaev
authors.forename: P V
authors.initials: PV
authors.lastname: Gil'chuk
authors.forename: O B
authors.initials: OB
authors.lastname: Gorbatiuk
authors.forename: A I
authors.initials: AI
authors.lastname: Fliak
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Labyntsev
authors.forename: D M
authors.initials: DM
authors.lastname: Irodov
authors.forename: D V
authors.initials: DV
authors.lastname: Kolibo
authors.forename: V A
authors.initials: VA
authors.lastname: Kordium
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D018952:Antigens, CD34 / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D018952:Antigens, CD34; D011993:Recombinant Fusion Proteins