title: emb-1 encodes the APC16 subunit of the Caenorhabditis elegans anaphase-promoting complex.
abstract: In the nematode Caenorhabditis elegans, temperature-sensitive mutants of emb-1 arrest as one-cell embryos in metaphase of meiosis I in a manner that is indistinguishable from embryos that have been depleted of known subunits of the anaphase-promoting complex or cyclosome (APC/C). Here we show that the emb-1 phenotype is enhanced in double mutant combinations with known APC/C subunits and suppressed in double mutant combinations with known APC/C suppressors. In addition to its meiotic function, emb-1 is required for mitotic proliferation of the germline. These studies reveal that emb-1 encodes K10D2.4, a homolog of the small, recently discovered APC/C subunit, APC16.
authors.affiliation: Department of Biology, College of William and Mary, Williamsburg, Virginia, USA.
authors.forename: Diane C
authors.initials: DC
authors.lastname: Shakes
authors.forename: Anna K
authors.initials: AK
authors.lastname: Allen
authors.forename: Kelsey M
authors.initials: KM
authors.lastname: Albert
authors.forename: Andy
authors.initials: A
authors.lastname: Golden
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D049109:Cell Proliferation; D029721:Drosophila Proteins; D005260:Female; D005816:Genetic Complementation Test; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics*
chemical_list: C467323:APC2 protein, Drosophila; D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D025521:Tumor Suppressor Proteins; C577471:emb-1 protein, C elegans; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome