title: Lymphocyte calcium influx kinetics in multiple sclerosis treated without or with interferon β.
abstract: Kv1.3 and IKCa1 potassium channels play an important role in the maintenance of calcium-influx during lymphocyte activation and present a possible target for selective immunomodulation. We investigated the calcium-influx characteristics of Th1, Th2, CD4, CD8 T-lymphocytes isolated from multiple sclerosis patients without or with interferon-beta therapy, and its modulation by Kv1.3 and IKCa1 channel inhibitors using flow cytometry. Specific immunomodulation of the CD8 subset can be reached through inhibition of Kv1.3 channels in multiple sclerosis patients without interferon-beta. However, this effect is not specific enough concerning all lymphocyte subsets influencing the autoimmune response, since it also affects anti-inflammatory Th2 cells.
authors.affiliation: First Department of Pediatrics, Semmelweis University, Budapest, Bókay u. 53-54, H-1083, Hungary. toldigergely@yahoo.com
authors.forename: Gergely
authors.initials: G
authors.lastname: Toldi
authors.forename: András
authors.initials: A
authors.lastname: Folyovich
authors.forename: Zsuzsa
authors.initials: Z
authors.lastname: Simon
authors.forename: Katalin
authors.initials: K
authors.lastname: Zsiga
authors.forename: Ambrus
authors.initials: A
authors.lastname: Kaposi
authors.forename: Gergo
authors.initials: G
authors.lastname: Mészáros
authors.forename: Tivadar
authors.initials: T
authors.lastname: Tulassay
authors.forename: Barna
authors.initials: B
authors.lastname: Vásárhelyi
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000627:therapeutic use*; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000276:immunology* / Q000473:pathology / Q000628:therapy*; D010865:Pilot Projects; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism; D055815:Young Adult
chemical_list: D016899:Interferon-beta; D002118:Calcium