title: LC determination of luteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in rat plasma after administration of Humulus scandens extract and its application to pharmacokinetic studies.
abstract: The present study was to investigate the pharmacokinetics of luteolin-7-O-β-D-glucoside (LGL) and apigenin-7-O-β-D-glucoside (AGL) in rat plasma after intravenous administration of the Humulus scandens extract (HSE). A simple and accurate high-performance liquid chromatographic (HPLC) method was successfully developed for simultaneous determination of LGL and AGL in rat plasma after the plasma protein was precipitated with methanol. HPLC analysis was performed on a C₁₈ column with UV detection at 350 nm and a mobile phase of methanol-0.2% phosphoric acid (1 : 1, v/v). Calibration curves of LGL and AGL were linear over the concentration range of 0.16-20.0 and 0.06-7.20 µg mL⁻¹, respectively. The accuracy and precision of the two analytes at low, medium and high concentrations were within the range of -3.4% to 8.1%. The relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 11.7% and 10.0%, respectively. The extraction recoveries (n = 5) varied from 91.9% to 104.1% for LGL and from 92.6% to 109.3% for AGL. The method was fully validated and successfully applied to a pharmacokinetic study of LGL and AGL in rat plasma after the intravenous administration of HSE.
authors.affiliation: School of Pharmaceutical Sciences, China Medical University, 92 Beier Road, Shenyang, Liaoning Province 110001, People's Republic of China.
authors.forename: Zaixing
authors.initials: Z
authors.lastname: Chen
authors.forename: Xixiang
authors.initials: X
authors.lastname: Ying
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.forename: Xu
authors.initials: X
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Qishen
authors.initials: Q
authors.lastname: Cao
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Fanhao
authors.initials: F
authors.lastname: Meng
mesh_terms: D000818:Animals; D047310:Apigenin / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D047309:Flavones / Q000097:blood*; D005960:Glucosides / Q000097:blood*; D027582:Humulus / Q000737:chemistry*; D007275:Injections, Intravenous; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D047311:Luteolin
chemical_list: D047310:Apigenin; D047309:Flavones; D005960:Glucosides; D010936:Plant Extracts; C057792:apigetrin; C066408:luteolin-7-glucoside; D047311:Luteolin