title: Involvement of REST corepressor 3 in prognosis of human hepatitis B.
abstract: OBJECTIVE
  To examine the potential correlation between serum REST corepressor 3 (RCOR3) level and the outcome of patients with hepatitis B.
  
  
  METHODS
  Concanavalin A (ConA)-induced mouse hepatitis model was used. The mRNA level of RCOR3 in mouse liver was measured using GeneChip array and real-time PCR. One hundred seventy-seven patients with hepatitis B and 34 healthy individuals were categorized into six groups including mild chronic hepatitis, moderate chronic hepatitis B, severe hepatitis B (SHB), cirrhosis, hepatocellular carcinoma (HCC) and healthy control. Serum levels of human RCOR3 were measured using ELISA.
  
  
  RESULTS
  In the mouse hepatitis model, the mRNA level of RCOR3 in liver was reduced early after exposure to ConA, then increased after 6 h of exposure. There was no significant difference in the serum RCOR3 level between the mild chronic hepatitis B and the control groups. The serum RCOR3 level was significantly increased in the moderate chronic hepatitis B group, but significantly reduced in SHB, cirrhosis and HCC groups, as compared with the control group. Moreover, the serum RCOR3 levels in SHB, cirrhosis and liver cancer patients were significantly lower than those in the patients with moderate chronic hepatitis B and with mild chronic hepatitis B. Rank correlation analysis revealed a significant correlation between serum RCOR3 level and total bilirubin (r=-0.305, P<0.01). There was no significant correlation between RCOR3 on one hand, and alanine transaminase (r=0.014, P>0.05) or aspartate transaminase (r=-0.079, P>0.05) on the other hand.
  
  
  CONCLUSIONS
  Serum RCOR3 level may reflect the degree of liver damage, which might be a potential biomarker for the outcome of patients with hepatitis B.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Ji-hua
authors.initials: JH
authors.lastname: Xue
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Xu
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000818:Animals; D056970:Co-Repressor Proteins; D003208:Concanavalin A / Q000008:administration & dosage; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics
chemical_list: D056970:Co-Repressor Proteins; D009419:Nerve Tissue Proteins; C121019:RCOR1 protein, human; D012333:RNA, Messenger; C000590777:Rcor3 protein, mouse; D012097:Repressor Proteins; D003208:Concanavalin A