title: Palladin is a novel binding partner of ILKAP in eukaryotic cells.
abstract: Palladin was a novel binding partner of ILKAP in eukaryotic cells. Palladin's C-terminal fragment including only its last three Ig domains (residues 710-1106) and the PP2C domain of ILKAP (residues 108-392) were necessary and sufficient for their interaction. The biological significance of the interaction between palladin and ILKAP was that palladin recruited the cytoplasmic ILKAP to initiate ILKAP-induced apoptosis. Our results suggested that palladin played a specific role in modulating the subcellular localization of the cytoplasmic ILKAP and promoting the ILKAP-induced apoptosis.
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhou
authors.forename: Shusen
authors.initials: S
authors.lastname: Cui
authors.forename: Shuhai
authors.initials: S
authors.lastname: Han
authors.forename: Baiqi
authors.initials: B
authors.lastname: Cheng
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yingjiu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D019556:COS Cells; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000201:enzymology; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000378:metabolism; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D003598:Cytoskeletal Proteins; C412722:PALLD protein, human; D010750:Phosphoproteins; C426826:ILKAP protein, human; D010749:Phosphoprotein Phosphatases