title: Expression and localization of CXCL16 and CXCR6 in ovarian endometriotic tissues.
abstract: OBJECTIVE
  Inflammatory mediators, including chemokines, may play crucial roles in the development of endometriosis. Therefore, we investigated the expression and localization of CXCL16 and its receptor, CXCR6, in ovarian endometriotic tissues. We also examined whether CXCL16 induces IL-8 production in endometriotic stromal cells.
  
  
  METHODS
  We performed immunohistochemical and Western blotting analyses of in vivo and in vitro samples. IL-8 production was assayed using an ELISA.
  
  
  RESULTS
  Both CXCL16 and CXCR6 were expressed by endometriotic epithelial cells and stromal cells, but not normal ovarian stroma. A Western blotting analysis using primary cultured endometriotic stromal cells showed a constant expression of CXCL16 and CXCR6 in the proliferative phase, secretory phase and during gonadotropin-releasing hormone agonist therapy. CXCL16 induced IL-8 production in several endometriotic stromal cells in vitro.
  
  
  CONCLUSIONS
  CXCL16 and CXCR6 might be involved in the pathophysiology of endometriosis through regulation of the inflammatory response.
authors.affiliation: Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Manabe
authors.forename: Akira
authors.initials: A
authors.lastname: Iwase
authors.forename: Maki
authors.initials: M
authors.lastname: Goto
authors.forename: Hiroharu
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Sachiko
authors.initials: S
authors.lastname: Takikawa
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Nagatomo
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakahara
authors.lastname: Bayasula
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nakamura
authors.forename: Wakana
authors.initials: W
authors.lastname: Hirokawa
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kikkawa
mesh_terms: D000328:Adult; D015153:Blotting, Western; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC / Q000378:metabolism*; D004715:Endometriosis / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000378:metabolism; D008875:Middle Aged; D010048:Ovarian Cysts / Q000378:metabolism*; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000378:metabolism*; D051116:Receptors, Scavenger / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
chemical_list: C423727:CXCL16 protein, human; C106330:CXCR6 protein, human; D000075743:Chemokine CXCL16; D019743:Chemokines, CXC; D016209:Interleukin-8; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D051116:Receptors, Scavenger; D011991:Receptors, Virus