title: Transcriptome profiling and insilico analysis of Gynostemma pentaphyllum using a next generation sequencer.
abstract: Gynosaponins (Gypenosides) are major phyto-chemicals in Gynostemma pentaphyllum (Thunb.), with similarities to the ginsenosides present in Panax ginseng. Gynosaponins are classified as terpenoid compounds. In G. pentaphyllum, 25% of the total gynosaponins are similar to ginsenosides. In this study, we analyzed the transcriptional levels of the G. pentaphyllum genome to identify secondary metabolite genes. The complete transcriptomes for the roots and leaves were obtained using a GS-FLX pyro-sequencer. In total, we obtained 265,340 and all reads were well annotated according to biological databases. Using insilico analysis, 84% of sequence were well annotated and we obtained most of the secondary metabolite genes that represent mono-, di-, tri- and sesquiterpenoids. From our EST, most of the terpenoid genes were noted, among those few similar genes were studied in P. ginseng and these transcripts will help to characterize more triterpenoid genes in G. pentaphyllum. Also help to compare P. ginseng and G. pentaphyllum at transcriptome level.
authors.affiliation: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, 449-701, South Korea.
authors.forename: Sathiyamoorthy
authors.initials: S
authors.lastname: Subramaniyam
authors.forename: Ramya
authors.initials: R
authors.lastname: Mathiyalagan
authors.forename: In
authors.initials: I
authors.lastname: Jun Gyo
authors.forename: Lee
authors.initials: L
authors.lastname: Bum-Soo
authors.forename: Lee
authors.initials: L
authors.lastname: Sungyoung
authors.forename: Yang
authors.initials: Y
authors.lastname: Deok Chun
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics; D029774:Gynostemma / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020005:Propanols / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000295:instrumentation* / Q000379:methods*; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D020005:Propanols; D012333:RNA, Messenger; C439395:1-phenylpropanol