title: Isolation and identification of bioactive antibacterial components in leaf extracts of Vangueria spinosa (Rubiaceae).
abstract: OBJECTIVE
  The column chromatographic fraction of ethyl acetate (EA1, EA2, EA3, EA4 and EA5) leaf extracts of Vangueria spinosa (V. spinosa) were screened for antibacterial activity and phytochemical analysis.
  
  
  METHODS
  EA3 fraction was isolated and identified by column chromatography, thin layer chromatography, spectral data analysis and phytochemical screening were used for analysis.
  
  
  RESULTS
  EA3 fraction was significantly active at 4 to 64 mg/L against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.1250 mg/mL. The active fraction (EA3) revealed the presence of flavonoid with retention factor value (R(f)) of 0.39. The active antibacterial agent in the most potent fraction (EA3) was isolated and identified as flavonoid (-)-epicatechin-3-O-β-glucopyranoside by thin layer chromatography (TLC) and phytochemical screening. EA1 and EA2 show inhibitory activity at 4 to 64 mg/L against Staphylococcus aureus only where as fraction EA4 and EA5 do not shows any inhibitory activity within that range of concentration against any bacteria.
  
  
  CONCLUSIONS
  The results support the ethnomedicinal use of leaf of V. spinosa for the treatment of bacterial diseases.
authors.affiliation: Mosquito and Microbiology Research Units, Parasitology Laboratory, Department of Zoology, The University of Burdwan West Bengal, India.
authors.forename: Soroj Kumar
authors.initials: SK
authors.lastname: Chatterjee
authors.forename: Indranil
authors.initials: I
authors.lastname: Bhattacharjee
authors.forename: Goutam
authors.initials: G
authors.lastname: Chandra
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D010936:Plant Extracts