title: Low-density microarrays for the detection of Borrelia burgdorferi s.s. (the Lyme disease spirochete) in nymphal Ixodes scapularis.
abstract: Lyme disease is the most common tick-borne disease in Europe and North America. In the hyperendemic Lyme disease regions of the eastern United States, nymphal Ixodes scapularis are the principal ticks transmitting the Lyme disease spirochete, Borrelia burgdorferi sensu stricto (s.s.). Approximately 25% of questing nymphs in endemic regions are infected with spirochetes. High throughput-sensitive and specific methods for testing nymphal I. scapularis for infection with B. burgdorferi are clearly needed. In the current study, we evaluated whether low-density microarrays could be adapted for the rapid and accurate detection and characterization of spirochetes in nymphal I. scapularis. Three different microarray platforms were developed and tested for the detection of spirochetes in ticks. They could both detect and differentiate different Borrelia genospecies, in one case detecting as few as a single copy of Borrelia DNA.
authors.affiliation: Department of Chemistry and Biochemistry, UCB 215, University of Colorado, Boulder, CO 80309, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Houck
authors.forename: Andrias
authors.initials: A
authors.lastname: Hojgaard
authors.forename: Joseph
authors.initials: J
authors.lastname: Piesman
authors.forename: Robert D
authors.initials: RD
authors.lastname: Kuchta
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001096:Arachnid Vectors / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000235:genetics; D025065:Borrelia burgdorferi / Q000145:classification* / Q000235:genetics; D018884:Ixodes / Q000382:microbiology*; D008074:Lipoproteins / Q000235:genetics; D009758:Nymph / Q000382:microbiology; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010727:Phosphoric Diester Hydrolases / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; C072045:OspA protein; C076733:FlaD protein, Bacteria; D010727:Phosphoric Diester Hydrolases; C087921:glpQ protein, Bacteria