title: [Pharmacokinetics of three phenolic acids of xiangdan injection in rats].
abstract: OBJECTIVE
  To establish a HPLC method for the analysis of Danshensu (DSS), protocatechuic acid (PA) and protocatechuic aldehyde (PAL) of Xiangdan injection in rat's plasma, and to study pharmacokinetic characteristics of Xiangdan injection components in rats with m-hydroxybenzoic acid as internal standard.
  
  
  METHODS
  protein was precipitated by 10% trichloroacetic acid and extracted by ethyl acetate. The plasma concentration was detected by HPLC, The pharmacokinetics parameters of DSS, PA and PAL were calculated by DAS2.0 software after iv injection.
  
  
  RESULTS
  DSS, PA and PAL have a good linear relationship in 0.68-44.0 mg x L(-1), 0.43-14.0 mg x L(-1) and 0.38-12.0 mg x L(-1), respectively. The average recoveries were more than 92% and the RSD of precision and stability of the test were between 0.4%-4.8%. DSS, PA and PAL showed a two-compartment open model, the half-life of absorption (t(1/2alpha)) were (6.26 +/- 4.6), (5.93 +/- 4.9), (18.44 +/- 2.4) min, the half-life of elimination (t(1/2beta)) were (64.11 +/- 8.8), (63.28 +/- 0.13), (69.315 +/- 0) min, the area under curve(AUC(0-infinity)) were (852.98 +/- 175.6), (83.84 +/- 58.8), (147.79 +/- 12.3) mg x min(-1) x L(-1).
  
  
  CONCLUSIONS
  A method with high recovery rate and good stability was established to determine the blood concentration of DSS, PA, PAL in Xiangdan injection and applied in its pharmacokinetics successfully.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Zhijuan
authors.initials: Z
authors.lastname: Gao
authors.forename: Bo
authors.initials: B
authors.lastname: Sang
authors.forename: Yangjing
authors.initials: Y
authors.lastname: Liang
authors.forename: Shixiang
authors.initials: S
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D005260:Female; D062385:Hydroxybenzoates / Q000097:blood / Q000493:pharmacokinetics*; D007267:Injections; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; C548756:xiangdan; C017616:phenolic acid