title: Characterization of components of the Staphylococcus aureus mRNA degradosome holoenzyme-like complex.
abstract: Bacterial two-hybrid analysis identified the Staphylococcus aureus RNA degradosome-like complex to include RNase J1, RNase J2, RNase Y, polynucleotide phosphorylase (PNPase), enolase, phosphofructokinase, and a DEAD box RNA helicase. Results also revealed that the recently recognized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in other Gram-positive organisms.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester Medical Center, School of Medicine and Dentistry, Box 672, 601 Elmwood Avenue, Rochester, NY 14642, USA.
authors.forename: Christelle M
authors.initials: CM
authors.lastname: Roux
authors.forename: Jonathon P
authors.initials: JP
authors.lastname: DeMuth
authors.forename: Paul M
authors.initials: PM
authors.lastname: Dunman
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D025221:Phosphofructokinases / Q000235:genetics / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020365:RNA Helicases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C111244:degradosome; D025221:Phosphofructokinases; D011117:Polyribonucleotide Nucleotidyltransferase; D004722:Endoribonucleases; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases; D010751:Phosphopyruvate Hydratase