title: Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation.
abstract: Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.
authors.affiliation: Departamento de Engenharia de Alimentos, Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de Erechim, Av. 7 de Setembro, Erechim, RS, Brazil.
authors.forename: Luciane
authors.initials: L
authors.lastname: Batistella
authors.forename: Mara K
authors.initials: MK
authors.lastname: Ustra
authors.forename: Aline
authors.initials: A
authors.lastname: Richetti
authors.forename: Sibele B C
authors.initials: SB
authors.lastname: Pergher
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
authors.forename: J V
authors.initials: JV
authors.lastname: Oliveira
authors.forename: Lindomar
authors.initials: L
authors.lastname: Lerin
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins; D008049:Lipase / Q000737:chemistry*; D009795:Octanes / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013016:Sound; D013696:Temperature; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D009795:Octanes; D012997:Solvents; C093666:Lipozyme; C093667:Novozyme 435; D008049:Lipase; C045798:2,2,4-trimethylpentane