title: Probing orientational behavior of MHC class I protein and lipid probes in cell membranes by fluorescence polarization-resolved imaging.
abstract: Steady-state polarization-resolved fluorescence imaging is used to analyze the molecular orientational order behavior of rigidly labeled major histocompatibility complex class I (MHC I) proteins and lipid probes in cell membranes of living cells. These fluorescent probes report the orientational properties of proteins and their surrounding lipid environment. We present a statistical study of the molecular orientational order, modeled as the width of the angular distribution of the molecules, for the proteins in the cell endomembrane and plasma membrane, as well as for the lipid probes in the plasma membrane. We apply this methodology on cells after treatments affecting the actin and microtubule networks. We find in particular opposite orientational order changes of proteins and lipid probes in the plasma membrane as a response to the cytoskeleton disruption. This suggests that MHC I orientational order is governed by its interaction with the cytoskeleton, whereas the plasma membrane lipid order is governed by the local cell membrane morphology.
authors.affiliation: Aix-Marseille Université, Ecole Centrale Marseille, Centre National de la Recherche Scientifique, Institut Fresnel, Marseille, France.
authors.forename: Alla
authors.initials: A
authors.lastname: Kress
authors.forename: Patrick
authors.initials: P
authors.lastname: Ferrand
authors.forename: Hervé
authors.initials: H
authors.lastname: Rigneault
authors.forename: Tomasz
authors.initials: T
authors.lastname: Trombik
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: He
authors.forename: Didier
authors.initials: D
authors.lastname: Marguet
authors.forename: Sophie
authors.initials: S
authors.lastname: Brasselet
mesh_terms: D000818:Animals; D016880:Anisotropy; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D005454:Fluorescence Polarization / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D008958:Models, Molecular
chemical_list: D005456:Fluorescent Dyes; D015395:Histocompatibility Antigens Class I; D008055:Lipids; D049452:Green Fluorescent Proteins