title: Development of a simple model device for in vitro gastric digestion investigation.
abstract: There have been some reports in the literature of model gastric digestion systems to mimic the dynamic physiological processes within the gastrointestinal tract. However, such devices often require the specification of many control parameters making routine digestion tests unfeasible. This paper introduces a simple in vitro digestion device, comprising of a water-jacketed glass vessel into which a spherical Teflon probe of variable diameter can be inserted. The probe is controlled by a texture analyser to simulate the kinetics of a food digestion process. Using this device under well controlled hydrodynamic flow and biochemical conditions key digestion parameters such as pH, food particle size, protein release, lipid release, cloudiness, etc, can be determined. Feasibility tests of the model device have been conducted using roasted and non-roasted peanuts particles. The status of peanut digestion was examined by the changes in particle size distribution and the mean particle size. Significant differences of surface microstructure have also been observed for peanut particles after the digestion. The influence of parameters such as food to gastric juice ratio, the probe speed and pepsin concentration have been examined in this work. Initial results confirm that all these factors influence the kinetic process of gastric digestion considerably and should be well regulated in any in vitro digestion investigations. We propose that the model device has the advantages of easy control and operation and furthermore could be an ideal tool for routine in vitro gastric digestion studies.
authors.affiliation: School of Food Science, University of Leeds, Leeds, LS2 9JT, UK. j.chen@food.leeds.ac.uk
authors.forename: Jianshe
authors.initials: J
authors.lastname: Chen
authors.forename: Vishwajeet
authors.initials: V
authors.lastname: Gaikwad
authors.forename: Melvin
authors.initials: M
authors.lastname: Holmes
authors.forename: Brent
authors.initials: B
authors.lastname: Murray
authors.forename: Malcolm
authors.initials: M
authors.lastname: Povey
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D010367:Arachis / Q000378:metabolism; D003198:Computer Simulation; D003938:Diagnostic Techniques, Digestive System / Q000295:instrumentation*; D004063:Digestion; D005240:Feasibility Studies; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000032:analysis; D008954:Models, Biological; D010316:Particle Size; D010434:Pepsin A / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism
chemical_list: D008055:Lipids; D011506:Proteins; D010434:Pepsin A