title: 2-Methoxyestradiol induced Bax phosphorylation and apoptosis in human retinoblastoma cells via p38 MAPK activation.
abstract: Retinoblastoma (Rb) is a common childhood intraocular cancer that affects approximately 300 children each year in the United States alone. 2-Methoxyestradiol (2ME), an endogenous metabolite of 17-β-estradiol that dose not bind to nuclear estrogen receptor, exhibits potent apoptotic activity against rapidly growing tumor cells. Here, we report that 2ME induction of apoptosis was demonstrated by early fragmented DNA after 48 h of incubation with 10 µM 2ME in Rb cell lines. Subsequently, a decrease of proliferation was observed in a time- and dose-dependent manner. Further analysis of the mechanism indicates that p38 kinase plays a critical role in 2ME-induced apoptosis in Y79 cells, even though ERK was also activated by 2ME under the same conditions. Activation of p38 kinase also mediates 2ME induced Bax phosphorylated at Thr(167) after a 6 h treatment of 2ME, which in turn prevents formation of the Bcl-2-Bax heterodimer. Both p38 specific inhibitor, SB 203580, or p38 knockdown by specific siRNA, blocked 2ME induction of Bax phosphorylation. Furthermore, only transiently transfected mutant BaxT167A, but not Bax S163A, inhibited 2ME-induced apoptosis. In summary, our data suggest that 2ME induces apoptosis in human Rb cells by causing phosphorylation of p38 Mitogen-activated protein kinase (MAPK), which appears to be correlated with phosphorlation of Bax. This understanding of 2ME's ability may help develop it as a promising therapeutic candidate by inducing apoptosis in a Rb.
authors.affiliation: Division of Ophthalmology, 455 Hospital, PLA, Shanghai, PR China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Min
authors.forename: Gautam S
authors.initials: GS
authors.lastname: Ghatnekar
authors.forename: Angela V
authors.initials: AV
authors.lastname: Ghatnekar
authors.forename: Xiaohong
authors.initials: X
authors.lastname: You
authors.forename: Min
authors.initials: M
authors.lastname: Bu
authors.forename: Xinyi
authors.initials: X
authors.lastname: Guo
authors.forename: Shizhong
authors.initials: S
authors.lastname: Bu
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Qin
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000077584:2-Methoxyestradiol; D017209:Apoptosis / Q000187:drug effects*; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D017931:DNA Primers; D004789:Enzyme Activation; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D034741:RNA, Small Interfering; D012175:Retinoblastoma / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; D051028:bcl-2-Associated X Protein; D004958:Estradiol; D000077584:2-Methoxyestradiol; D048051:p38 Mitogen-Activated Protein Kinases