title: Construction of a triglyceride amperometric biosensor based on chitosan-ZnO nanocomposite film.
abstract: A method is described for construction of a novel amperometric triglyceride (TG) biosensor based on covalent co-immobilization of lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO) onto chitosan (CHIT) and zinc oxide nanoparticles (ZnONPs) composite film deposited on the surface of Pt electrode. The enzymes-ZnONPs-CHIT composite was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor showed optimum response within 6s at pH 7.5 and temperature of 35°C. The sensor measures current due to electrons generated at 0.4V against Ag/AgCl from H(2)O(2), which is produced from triolein by co-immobilized enzymes. A linear relationship was obtained between a wide triolein concentration range (50-650 mg/dl) and current (mA) under optimum conditions. The biosensor showed high sensitivity, low detection limit (20 mg/dl) and good storage stability (half-life of 7 months at 4°C). The biosensor was unaffected modified by a number of serum substances at their physiological concentrations. The biosensor was evaluated and employed for determination of TG in sera in apparently healthy subjects and persons suffering from hypertriglyceridemia.
authors.affiliation: Department of Biochemistry, MD University, Rohtak, Haryana, India.
authors.forename: Jagriti
authors.initials: J
authors.lastname: Narang
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D004795:Enzyme Stability; D005260:Female; D005991:Glycerol Kinase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008297:Male; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D014280:Triglycerides / Q000097:blood*; D014304:Triolein / Q000032:analysis; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D014280:Triglycerides; D014304:Triolein; D048271:Chitosan; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase; D005991:Glycerol Kinase; D015034:Zinc Oxide