title: [Effect of chlorogenic acid on degranulation in mast cell RBL-2H3].
abstract: OBJECTIVE
  To provide evidences for evaluating the role of chlorogenic acid (CA) on the adverse reaction of traditional Chinese medicine injection and promoting clinical rational usage of CA, the effect of CA and chlorogenic acid-HSA(CA-HSA) on the degranulation in mast cell RBL-2H3 were compared and the allergenic effect and its mechanism were investigated.
  
  
  METHODS
  The unsensitized and sensitized RBL-2H3 cells were used. The releasing rate of histamine and beta-hexosaminidase was detected by colormetric assays. The degranulating rate was detected by neutral red staining and Annexin V positive cell rate was detected by flow cytometry.
  
  
  RESULTS
  CA and CA-HSA could not induce degranulation in unsensitized RBL-2H3 cells. CA and CA-HSA could significantly increase the release of histamine and beta-hexosaminidase, degranulating rate and Annexin V positive cell rate.
  
  
  CONCLUSIONS
  CA has strong allergenicity after combination with serum proteins. As an active ingredient of Shuanghuanglian injection, CA is a kind of possible allergen which caused hypersensitivity reactions induced by Shuanghuanglian injection.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Qihua
authors.initials: Q
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Yanli
authors.initials: Y
authors.lastname: Lu
authors.forename: Jianrong
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects*; D002460:Cell Line; D002726:Chlorogenic Acid / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000009:adverse effects*; D006636:Histamine Release / Q000187:drug effects; D008407:Mast Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid; D001619:beta-N-Acetylhexosaminidases