title: N-acetylglucosaminyltransferase V-deficiency increases susceptibility to murine malaria.
abstract: It is considered that several glycoproteins on erythrocytes in mammalian species are involved in malaria parasite infection. To elucidate the role of N-glycans on malaria parasite infection, we induced experimental murine malaria infection (using Plasmodium berghei ANKA) in mice deficient in N-acetylglucosaminyltransferase V (Mgat5), which is one of the enzymes involved in β1,6-GlcNAc N-glycan biosynthesis. After infection, Mgat5(-/-) mice showed severe body weight loss and parasitemia compared with wild-type mice. The Mgat5(-/-) mice, but not wild-type mice, also showed severe pathology accompanied by marked infiltration of plasma cells into the lungs and liver. These results suggest that β1,6-GlcNAc N-glycans on/in host erythrocytes may interfere with invasion of the parasites and progression to severe malaria.
authors.affiliation: Department of Medical Genomics, Graduate School of Frontier Sciences, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. shibui@mgs.k.u-tokyo.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Shibui
authors.forename: Junko
authors.initials: J
authors.lastname: Doi
authors.forename: Mohammed E M
authors.initials: ME
authors.lastname: Tolba
authors.forename: Chiharu
authors.initials: C
authors.lastname: Shiraishi
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sato
authors.forename: Shumpei
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Junichi
authors.initials: J
authors.lastname: Watanabe
authors.forename: Sadao
authors.initials: S
authors.lastname: Nogami
authors.forename: Susumu
authors.initials: S
authors.lastname: Nakae
authors.forename: Sumio
authors.initials: S
authors.lastname: Sugano
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Hozumi
mesh_terms: D000818:Animals; D004198:Disease Susceptibility / Q000201:enzymology; D004912:Erythrocytes / Q000469:parasitology; D005260:Female; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D017351:N-Acetylglucosaminyltransferases / Q000172:deficiency*; D018512:Parasitemia / Q000276:immunology; D010962:Plasmodium berghei; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000276:immunology
chemical_list: D007371:Interferon-gamma; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase