title: Survival of Burkholderia pseudomallei in distilled water for 16 years.
abstract: Burkholderia pseudomallei was examined after being maintained in distilled water at 25°C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8×10(7) cells/ml and 1.4×10(5) cells/ml, respectively. A colony count on agar of 1.0×10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand.
authors.forename: Apinya
authors.initials: A
authors.lastname: Pumpuang
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.forename: Chanthiwa
authors.initials: C
authors.lastname: Wikraiphat
authors.forename: Natnaree
authors.initials: N
authors.lastname: Saiprom
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
authors.forename: Vanaporn
authors.initials: V
authors.lastname: Wuthiekanun
mesh_terms: D016957:Burkholderia pseudomallei / Q000235:genetics / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008554:Melioidosis / Q000235:genetics*; D013997:Time Factors; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial