title: Creation of novel signalling modulators from existing cytokine using scanning motif-programming.
abstract: We created two artificial modulating proteins for bone morphogenetic protein (BMP)-signalling, tmw57 and tmw61, starting from partial sequences of human BMP-2 (hBMP-2). The proteins were able to boost the hBMP-4 activities, BMP responsive element-driven transcription and an osteoblastic differentiation marker induction.
authors.affiliation: Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, Koto, Tokyo 135-8550, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Murakami
authors.forename: Kenji
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shiba
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000378:metabolism*; D052004:Bone Morphogenetic Protein Receptors / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D015398:Signal Transduction
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D052004:Bone Morphogenetic Protein Receptors