title: Plasmid profile analysis of Portuguese Borrelia lusitaniae strains.
abstract: Plasmid profiles of 2 Portuguese Borrelia lusitaniae strains, one isolated from a human patient and the other one from an Ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. Overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of B. burgdorferi sensu lato, the causative agents of Lyme borreliosis. The plasmid location of the ospA gene was investigated by hybridization, allowing its allocation to the plasmid of 70 kb instead of the 54 kb linear plasmid described for B. burgdorferi sensu stricto strains.
authors.affiliation: Universidade de Lisboa, Faculdade de Ciências, Centro de Genética e Biologia Molecular and Instituto de Ciência Aplicada e Tecnologia, Lisboa, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Vitorino
authors.forename: Gabriele
authors.initials: G
authors.lastname: Margos
authors.forename: Líbia
authors.initials: L
authors.lastname: Zé-Zé
authors.forename: Klaus
authors.initials: K
authors.lastname: Kurtenbach
authors.forename: Margarida
authors.initials: M
authors.lastname: Collares-Pereira
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000235:genetics*; D001898:Borrelia / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008074:Lipoproteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011174:Portugal; D017422:Sequence Analysis, DNA
chemical_list: D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D008074:Lipoproteins; C072045:OspA protein