title: [Changes of leukotriene D4 in nasal discharge and plasma of patients with persistent allergic rhinitis and the effects of antihistamine].
abstract: OBJECTIVE
  To investigate the changes of leukotriene D4 (LTD4) in nasal discharge and plasma of patients with persistent allergic rhinitis (AR) and the effects of antihistamine.
  
  
  METHODS
  The investigation was a prospective, randomized controlled trial. Forty AR patients (group C) were divided randomly into two subgroup. One group received oral antihistamine 10 mg everyday for one week (group CA) and another group received no loratadine tablets 10 mg everyday for one week (group CB). Fifteen age matched healthy (group D) people were enrolled as control. The level of LTD4 and interleukin-5 (IL-5) in both nasal discharge and plasma by using enzyme linked immunosorbent assay (ELISA) and enzyme immunoassay (EIA), cell counts and cell differentials in nasal discharge, were measured before and after three month. The clinical symptom and life quality scores of group C were also investigated.
  
  
  RESULTS
  The concentrations of LTD4 in nasal discharge [(794 +/- 305) pg] and plasma [(5219 +/- those in group D [(347 +/- 169) pg, (2283 +/- 489) ng/L, all P 1185) ng/L] in group C were significantly higher than those in group D [(347 +/- 169) pg, (2283 +/- 489) ng/L, all P < 0.05]. The level of LTD4 in nasal discharge was positively correlated with the percentage of neutrophil (r = 0.453, P < 0.05) and IL-5 (r = 0.364, P < 0.05). The pre- and post-therapy concentrations of nasal discharge and plasma in group CA were (812 +/- 1592) pg, (657 +/- 495) pg and (5422 +/- 935) ng/L, (4589 +/- 1057) ng/L respectively; While in group CB the concentrations were (776 +/- 227) pg, (860 +/- 194) pg and (5074 +/- 1850) ng/L, (6063 +/- 450) ng/L, respectively. There were no significant difference either in the level of LTD4 in nasal discharge or in plasma in both groups (all P > 0.05).
  
  
  CONCLUSIONS
  The results suggested that LTD4 was involved in airway inflammation in AR. Antihistamine was not effective enough in decreasing the levels of LTD4 in both nasal discharge and plasma of AR patients.
authors.affiliation: Department of Otorhinolaryngology, Xiangyang Hospital, Tongji Medical College, Huazhong University of Science and Technology, the Central Hospital of Xiangyang, 441021, China. lh07103999370@163.com
authors.forename: Hong
authors.initials: H
authors.lastname: Luo
authors.forename: Ye
authors.initials: Y
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Shen
authors.forename: Ligang
authors.initials: L
authors.lastname: Zeng
authors.forename: Nengbing
authors.initials: N
authors.lastname: Yan
authors.forename: Feng
authors.initials: F
authors.lastname: Ke
authors.forename: Xuanxiang
authors.initials: X
authors.lastname: Fu
authors.forename: Pengju
authors.initials: P
authors.lastname: Wang
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
mesh_terms: D000328:Adult; D018926:Anti-Allergic Agents / Q000494:pharmacology; D012634:Bodily Secretions / Q000737:chemistry; D005260:Female; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006801:Humans; D020024:Leukotriene Antagonists / Q000627:therapeutic use*; D017998:Leukotriene D4 / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry; D011446:Prospective Studies; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism*
chemical_list: D018926:Anti-Allergic Agents; D006634:Histamine H1 Antagonists; D020024:Leukotriene Antagonists; D017998:Leukotriene D4