title: Synchronization of pathogenic protozoans.
abstract: Protozoans are single-cell eukaryotes and many of the best studied protozoans are parasitic to humans (e.g., Plasmodium falciparum causing malaria and Trypanosoma brucei causing sleeping sickness). These organisms are distantly related to humans but with retained eukaryotic type of cellular processes, making them good model systems for studies of the evolution of basic processes like the cell cycle. Giardia intestinalis causes 250 million cases of diarrhea yearly and is one of the earliest diverging protozoans. It has recently been possible to synchronize its cell cycle using compounds that inhibit different steps of the cell cycle and the detailed protocol is described here.
authors.affiliation: Department of Cell and Molecular Biology, Uppsala University, Uppsala, SE-5421, Sweden. staffan.svard@icm.uu.se
authors.forename: Staffan
authors.initials: S
authors.lastname: Svärd
authors.forename: Karin
authors.initials: K
authors.lastname: Troell
mesh_terms: D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D005434:Flow Cytometry; D016829:Giardia lamblia / Q000187:drug effects / Q000254:growth & development*; D050258:Mitosis Modulators / Q000494:pharmacology; D013194:Staining and Labeling
chemical_list: D050258:Mitosis Modulators