title: Dynamic compression of rabbit adipose-derived stem cells transfected with insulin-like growth factor 1 in chitosan/gelatin scaffolds induces chondrogenesis and matrix biosynthesis.
abstract: Articular cartilage is routinely subjected to mechanical forces and growth factors. Adipose-derived stem cells (ASCs) are multi-potent adult stem cells and capable of chondrogenesis. In the present study, we investigated the comparative and interactive effects of dynamic compression and insulin-like growth factor-I (IGF-I) on the chondrogenesis of rabbit ASCs in chitosan/gelatin scaffolds. Rabbit ASCs with or without a plasmid overexpressing of human IGF-1 were cultured in chitosan/gelatin scaffolds for 2 days, then subjected to cyclic compression with 5% strain and 1 Hz for 4 h per day for seven consecutive days. Dynamic compression induced chondrogenesis of rabbit ASCs by activating calcium signaling pathways and up-regulating the expression of Sox-9. Dynamic compression plus IGF-1 overexpression up-regulated expression of chondrocyte-specific extracellular matrix genes including type II collagen, Sox-9, and aggrecan with no effect on type X collagen expression. Furthermore, dynamic compression and IGF-1 expression promoted cellular proliferation and the deposition of proteoglycan and collagen. Intracellular calcium ion concentration and peak currents of Ca(2+) ion channels were consistent with chondrocytes. The tissue-engineered cartilage from this process had excellent mechanical properties. When applied together, the effects achieved by the two stimuli (dynamic compression and IGF-1) were greater than those achieved by either stimulus alone. Our results suggest that dynamic compression combined with IGF-1 overexpression might benefit articular cartilage tissue engineering in cartilage regeneration.
authors.affiliation: Department of Orthopaedics, Shengjing Hospital of China Medical University, Shenyang, P R China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Enbo
authors.initials: E
authors.lastname: Wang
authors.forename: Chuanhui
authors.initials: C
authors.lastname: Zhang
authors.forename: Guangbin
authors.initials: G
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014162:Transfection
chemical_list: D015220:Calcium Channels; D007334:Insulin-Like Growth Factor I; D005780:Gelatin; D048271:Chitosan; D002118:Calcium