title: Protection against enamel demineralisation using toothpastes containing o-cymen-5-ol, zinc chloride and sodium fluoride.
abstract: OBJECTIVE
  To evaluate the ability of two experimental toothpastes containing 0.1%w/w o-cymen-5-ol, 0.6%w/w ZnCl2 and 0.320%w/w NaF to reduce demineralisation of sound human enamel compared with control toothpastes.
  
  
  METHODS
  Study 1: Specimens were treated with toothpaste slurries, followed by alternating periods in demineralising and neutral solutions. Demineralisation was assessed using surface microhardness (SMH). Study 2: Specimens were subjected to a 14 day cycling regime of alternating demineralisation/remineralisation with two toothpaste treatments per day, before and after demineralisation. Demineralisation was assessed by cross-sectional microhardness and mineral loss (ΔZ) was calculated. Test toothpastes were a) 0%w/w or 0.002%w/w NaF placebo, b) 0.055%w/w or 0.149%w/w NaF (dose response), c) 0.320%w/w NaF marketed product, d & e) 0.1%w/w o-cymen-5-ol, 0.6%w/w ZnCl2 and 0.320%w/w NaF (experimental toothpastes).
  
  
  RESULTS
  Study 1: Mean±SE % of baseline hardness values were a) 48.0±2.1a, b) 66.7±1.7b, c) 82.9±1.9c, d) 91.7±1.4d and e) 94.6±2.1d. Study 2: Mean±SE ΔZ values were a) 2114±187a, b) 1206±132b, c) 303±89c, d) 19±73c, and e) -10±55c. Letters represent different statistical groupings (P<0.05).
  
  
  CONCLUSIONS
  In study 1, both experimental toothpastes were statistically superior to the marketed product and in study 2; they were at least as effective as the marketed product at reducing caries lesion development.
authors.affiliation: GlaxoSmithKline Consumer Healthcare, Weybridge, Surrey, UK. david.r.churchley@gsk.com
authors.forename: David
authors.initials: D
authors.lastname: Churchley
authors.forename: Craig S
authors.initials: CS
authors.lastname: Newby
authors.forename: Richard
authors.initials: R
authors.lastname: Willson
authors.forename: Amir
authors.initials: A
authors.lastname: Haider
authors.forename: Bruce
authors.initials: B
authors.lastname: Schemehorn
authors.forename: Richard J M
authors.initials: RJ
authors.lastname: Lynch
mesh_terms: D000704:Analysis of Variance; D002327:Cariostatic Agents / Q000494:pharmacology / Q000627:therapeutic use; D002712:Chlorides / Q000494:pharmacology / Q000627:therapeutic use*; D006244:Hardness / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000494:pharmacology / Q000627:therapeutic use*; D012969:Sodium Fluoride / Q000494:pharmacology / Q000627:therapeutic use; D017001:Tooth Demineralization / Q000517:prevention & control*; D014089:Tooth Remineralization; D014100:Toothpastes / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D017967:Zinc Compounds / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D002327:Cariostatic Agents; D002712:Chlorides; D010636:Phenols; D014100:Toothpastes; D017967:Zinc Compounds; C016837:zinc chloride; D012969:Sodium Fluoride; C512394:o-cymen-5-ol