title: Evaluation of Andrographis paniculata Burm.f. (Family:Acanthaceae) extracts against Culex quinquefasciatus (Say.) and Aedes aegypti (Linn.) (Diptera:Culicidae).
abstract: OBJECTIVE
  To investigate the larvicidal and ovicidal efficacy of different extracts of Andrographis paniculata (A. paniculata) against Culex quinquefasciatus (Cx. quinquefasciatus) Say and Aedes aegypti (Ae. aegypti) L. (Diptera: Culicidae).
  
  
  METHODS
  Larvicidal efficacy of the crude leaf extracts of A. paniculata with five different solvents like benzene, hexane, ethyl acetate, methanol and chloroform was tested against the early third instar larvae of Cx. quinquefasciatus and Ae. aegypti. The ovicidal activity was determined against two mosquito species to various concentrations ranging from 50-300 ppm under the laboratory conditions.
  
  
  RESULTS
  The benzene, hexane, ethyl acetate, methanol and chloroform leaf extract of A. paniculata was found to be more effective against Cx. quinquefasciatus than Ae. aegypti. The LC(50) values were 112.19, 137.48, 118.67, 102.05, 91.20 ppm and 119.58, 146.34, 124.24, 110.12, 99.54 ppm respectively. Among five tested solvent, methanol and ethyl acetate crude extract was found to be most effective for ovicidal activity against two mosquito species. The extract of methanol and ethyl acetate exerted 100% mortality at 200 ppm against Cx. quinquefasciatus and at 250 ppm against Ae. aegypti.
  
  
  CONCLUSIONS
  From the results it can be concluded the crude extract of A. paniculata was a potential for controlling Cx. quinquefasciatus and Ae. aegypti mosquitoes.
authors.affiliation: Division of Vector Biology and Phytochemistry, Department of Zoology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India. drgovind1979@rediffmail.com
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Govindarajan
mesh_terms: D000330:Aedes / Q000187:drug effects*; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D003465:Culex / Q000187:drug effects*; D007306:Insecticides / Q000302:isolation & purification* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D010063:Ovum / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D016019:Survival Analysis
chemical_list: D007306:Insecticides; D010936:Plant Extracts