title: Detection of hepatitis B virus DNA in paraffin-embedded intrahepatic and extrahepatic cholangiocarcinoma tissue in the northern Chinese population.
abstract: This study explored the importance of hepatitis B virus infection in cholangiocarcinoma pathogenesis in northern China. The clinical data of 66 patients with cholangiocarcinoma were analyzed. The hepatitis B virus gene was amplified using nested polymerase chain reaction, and the hepatitis B virus-related antigen was detected using immunohistochemistry in formalin-fixed, paraffin-embedded tissue from patients with intrahepatic cholangiocarcinoma (n = 23) and extrahepatic cholangiocarcinoma (n = 43). Hepatitis B surface antigen seropositivity was found in 52.2% (12/23) of intrahepatic cholangiocarcinoma cases and 13.9% (6/43) of extrahepatic cholangiocarcinoma cases. Hepatitis B virus DNA (X region) was detectable in 34.8% (8/23) of intrahepatic cholangiocarcinoma cases. Hepatitis B surface antigen and/or hepatitis B core antigen was detectable in 30.4% (7/23) of intrahepatic cholangiocarcinoma cases. All cases with detected viral protein were also positive for hepatitis B virus DNA. In contrast, no hepatitis B virus antigens or hepatitis B virus gene was detected in any of the 43 extrahepatic cholangiocarcinoma cases. Our findings strongly suggest that chronic hepatitis B virus infection is a significant risk factor for intrahepatic cholangiocarcinoma, but not for extrahepatic cholangiocarcinoma, in northern China. Hepatitis B virus infection is potentially independently associated with intrahepatic cholangiocarcinoma.
authors.affiliation: Department of Pathology, Basic Medical Science College, Harbin Medical University, Harbin 150081, China.
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Wu
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Wang
authors.forename: Shengqian
authors.initials: S
authors.lastname: Ye
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Bai
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.forename: Kenji
authors.initials: K
authors.lastname: Abe
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
mesh_terms: D002759:Adenoma, Bile Duct / Q000208:ethnology / Q000473:pathology / Q000821:virology*; D001650:Bile Duct Neoplasms / Q000208:ethnology / Q000473:pathology / Q000821:virology*; D001653:Bile Ducts, Intrahepatic / Q000473:pathology / Q000821:virology*; D002681:China / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006509:Hepatitis B / Q000150:complications* / Q000208:ethnology / Q000473:pathology; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding
chemical_list: D004279:DNA, Viral