title: Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells.
abstract: Cancer cells have been reported to exhibit an enhanced capacity for protoporphyrin IX (PpIX) synthesis facilitated by the administration of 5-aminolevulinic acid (ALA). We investigated the effect of ALA-based photodynamic therapy (PDT) on human cholangiocarcinoma cells (HuCC-T1). Since protoporphyrin IX (PpIX), a metabolite of ALA, can produce reactive oxygen species (ROS) under irradiation and then induce phototoxicity, ALA-based PDT is a promising candidate for the treatment of cholangiocarcinoma. When various concentrations of ALA (0.05-2 mM) were used to treat HuCC-T1 cells for 6 or 24 hours, the intracellular PpIX level increased according to the ALA concentration and treatment time. Furthermore, an increased amount of PpIX in HuCC-T1 cells induced increased production of ROS by irradiation, resulting in increased phototoxicity.
authors.affiliation: National Research and Development Center for Hepatobiliary Cancer, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, Yangsan, Republic of Korea.
authors.forename: Cy Hyun
authors.initials: CH
authors.lastname: Kim
authors.forename: Chung-Wook
authors.initials: CW
authors.lastname: Chung
authors.forename: Kyung Ha
authors.initials: KH
authors.lastname: Choi
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Yoo
authors.forename: Do Hyung
authors.initials: DH
authors.lastname: Kim
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Jeong
authors.forename: Dae Hwan
authors.initials: DH
authors.lastname: Kang
mesh_terms: D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D009336:Necrosis; D010778:Photochemotherapy / Q000379:methods*; D010784:Photomicrography; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011419:Propidium; D011524:Protoporphyrins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D017382:Reactive Oxygen Species; D011419:Propidium; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX