title: Hybridization experiments indicate incomplete reproductive isolating mechanism between Fasciola hepatica and Fasciola gigantica.
abstract: Experiments on hybridization between Fasciola hepatica and Fasciola gigantica were carried out to clarify whether a reproductive isolating mechanism appears between the two Fasciola species. Molecular evidence for hybridization was based on the DNA sequence of the internal transcribed spacer 1 (ITS1) region in nuclear ribosomal DNA, which differs between the species. The results suggested that there were not pre-mating but post-mating isolating mechanisms between the two species. However, viable adults of the hybrids F1 and F2 were produced from both parental F. hepatica and F. gigantica. The hybrids inherited phenotypic characteristics such as ratio of body length and width and infectivity to rats from parental Fasciola hepatica and F. gigantica. These findings suggest that reproductive isolation is incomplete between Fasciola hepatica and F. gigantica. Adults of the hybrids F1 and F2 were completely different in mode of reproduction from aspermic Fasciola forms that occur in Asia and seem to be offspring originated from hybridization between F. hepatica and F. gigantica and to reproduce parthenogenetically.
authors.affiliation: Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3-18-8, Morioka 020-8550, Japan. itagaki@iwate-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Itagaki
authors.forename: M
authors.initials: M
authors.lastname: Ichinomiya
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: S
authors.initials: S
authors.lastname: Fusyuku
authors.forename: C
authors.initials: C
authors.lastname: Carmona
mesh_terms: D000349:Africa; D000818:Animals; D001208:Asia; D002678:Chimera / Q000235:genetics*; D018509:DNA, Helminth / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005060:Europe; D005209:Fasciola / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D005210:Fasciola hepatica / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D005211:Fascioliasis / Q000469:parasitology* / Q000662:veterinary; D006041:Goats; D006824:Hybridization, Genetic; D058488:Metacercariae / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D009656:North America; D058974:Phylogeography; D012150:Polymorphism, Restriction Fragment Length; D060047:Reproductive Isolation; D017422:Sequence Analysis, DNA; D012756:Sheep; D012908:Snails; D013020:South America; D013045:Species Specificity
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer