title: Transcriptional fingerprint of human whole blood at the site of coronary occlusion in acute myocardial infarction.
abstract: OBJECTIVE
  Transcriptome patterns associated with acute myocardial infarction at the site of coronary occlusion are largely unknown. The aim of this study was to decipher the angiogenic, atherosclerotic, and inflammatory mRNA profiles in whole blood samples collected at the site of coronary occlusion in patients with ST-elevation myocardial infarction (STEMI).
  
  
  RESULTS
  In five consecutive patients with STEMI, blood was sampled at the site of occlusion (local) and in the systemic circulation (peripheral) during primary percutaneous coronary intervention. RNA was extracted from whole blood samples. Among 221 genes involved in angiogenesis, inflammation and atherosclerosis, 24 were shown to be differentially modulated locally, by analysis with custom-designed DNA array technology. Validation in 28 distinct STEMI patients using real-time quantitative PCR identified seven out of these 24 genes to be consistently and significantly upregulated in local versus peripheral blood (p<0.05). Three genes were chemokine family members (CCL2, CCL18 and CXCL12), three genes belonged to the cell-cell and cell-extracellular matrix family (FN1, CDH5 and SPP1), and one gene was representative of the lipoprotein family (APOE).
  
  
  CONCLUSIONS
  We identified a set of whole blood transcripts induced at the site of coronary occlusion in the acute phase of myocardial infarction. Resolved genes indicate a predominant role for chemokines, cell-extracellular matrix, and lipoprotein alterations in the pathophysiology of acute myocardial infarction and the initial response to myocardial injury.
authors.affiliation: Cardiovascular Center and Translational Cardiology Unit, Aalst, Belgium.
authors.forename: Olivier
authors.initials: O
authors.lastname: Muller
authors.forename: Leen
authors.initials: L
authors.lastname: Delrue
authors.forename: Michalis
authors.initials: M
authors.lastname: Hamilos
authors.forename: Steven
authors.initials: S
authors.lastname: Vercauteren
authors.forename: Argyrios
authors.initials: A
authors.lastname: Ntalianis
authors.forename: Catalina
authors.initials: C
authors.lastname: Trana
authors.forename: Fabio
authors.initials: F
authors.lastname: Mangiacapra
authors.forename: Karen
authors.initials: K
authors.lastname: Dierickx
authors.forename: Bernard
authors.initials: B
authors.lastname: De Bruyne
authors.forename: William
authors.initials: W
authors.lastname: Wijns
authors.forename: Atta
authors.initials: A
authors.lastname: Behfar
authors.forename: Emanuele
authors.initials: E
authors.lastname: Barbato
authors.forename: Andre
authors.initials: A
authors.lastname: Terzic
authors.forename: Marc
authors.initials: M
authors.lastname: Vanderheyden
authors.forename: Jozef
authors.initials: J
authors.lastname: Bartunek
mesh_terms: D000368:Aged; D042501:Angiogenic Proteins / Q000235:genetics; D015906:Angioplasty, Balloon, Coronary; D050197:Atherosclerosis / Q000235:genetics; D001530:Belgium; D016022:Case-Control Studies; D054059:Coronary Occlusion / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000628:therapy; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks; D006801:Humans; D007249:Inflammation / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000235:genetics* / Q000628:therapy; D020411:Oligonucleotide Array Sequence Analysis; D011446:Prospective Studies; D012333:RNA, Messenger / Q000097:blood*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014158:Transcription, Genetic
chemical_list: D042501:Angiogenic Proteins; D012333:RNA, Messenger