title: Human cytomegalovirus RL13 gene transcripts in a clinical strain.
abstract: Human cytomegalovirus (HCMV) RL13 gene is a member of the RL11 gene family. To study the expression kinetics and transcript structures of the gene, screening of cDNA library, Northern blot, 3' and 5' RACE analyses were performed with a low-passaged clinical strain. The results showed that the RL13 gene was mainly transcribed in the late expression phase with at least seven forms of transcripts of 3628, 3114, 2515-2443, 1737, 1240, 1029 and 846 nt, respectively. All these transcripts were unspliced with an identical 3' terminal and the same typical polyA signal "AATAAA". Except for the transcript of 846 nt, RL13 open reading frames (ORFs) were 909 bp and completely identical in all of the transcripts. The sequence of the RL13 ORF in the examined clinical strain had a higher similarity with that of the UL153 ORF in the Towne strain than those of RL13 ORF in any other HCMV strains.
authors.affiliation: China Medical University, Shenyang, Liaoning, People's Republic of China.
authors.forename: Mali
authors.initials: M
authors.lastname: Li
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Ji
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Gao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D001483:Base Sequence; D002681:China; D003587:Cytomegalovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology*; D005260:Female; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins