title: Age-related morphological impairments in the rat hippocampus following developmental lead exposure: an MRI, LM and EM study.
abstract: Lead is one of the most common neurotoxic metals present in our environment. Chronic developmental lead exposure is known to be associated with cognitive dysfunction in children. Functional and morphological impairment of the rat brain has also been reported in the hippocampus (Hi) following developmental lead exposure. The present study was carried out to further investigate age-related morphological impairments in the rat Hi following developmental lead exposure with three methods: (1) magnetic resonance imaging (MRI); (2) light microscopy (LM); and (3) electron microscopy (EM) techniques. Neonatal Wistar rats were exposed to lead from parturition to weaning via milk of dams drinking a 0.2% lead acetate solution. Age-related morphological alternations were investigated in the Hi of lead-exposed rats at various postnatal ages: postnatal day (PND) 17, 30 and 90. The MRI signal intensities (SIs) in the left, right, superior and inferior hippocampal regions of control and lead-exposed rats were analyzed. Compared with controls, the SIs of the four hippocampal regions of interest were significantly increased in lead-exposed rats at PND 17, 30 and 90. Moreover, the lead-induced impairment of the Hi showed an age-related decline and a specific topographical pattern. The impairment of inferior hippocampal regions was more severe than that of superior regions in lead-exposed rats at PND 17 and 30, while no significant difference of SIs was observed between left and right hippocampal regions in the three age groups, and between superior and inferior regions in the PND 90 lead-exposed rats. The LM observations indicated that the morphological injury of hippocampal neurons in lead-exposed rats was also age-related. The EM observations revealed that the endoplasmic reticular, Golgi complex and mitochondria of hippocampal CA1 and dentate gyrus neurons in lead-exposed rats were damaged. These results demonstrate that lead-induced morphological impairments of the rat Hi follow a specific age- and site-related pattern.
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Meng
authors.forename: Di-Yun
authors.initials: DY
authors.lastname: Ruan
authors.forename: Lian-Di
authors.initials: LD
authors.lastname: Kang
authors.forename: Da-Miao
authors.initials: DM
authors.lastname: Zhu
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: She
authors.forename: Le
authors.initials: L
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Li