title: A novel rolling circle amplification assay to detect members of the family Anelloviridae in pigs and humans.
abstract: The study describes a novel rolling circle amplification (RCA) assay to detect members of the family Anelloviridae from swine and human serum samples. The RCA was carried out using short anellovirus (AV)-specific primers based on a highly conserved region among available AV full-length genomes. Then, RCA products were used as templates to amplify full-length genomes with an AV-specific PCR. Amplification products were separated by agarose gel electrophoresis and full-length genomes were isolated based on the known size. With this novel AV-RCA/PCR approach it was possible to detect Torque teno sus virus 1 (TTSuV1) and Torque teno sus virus 2 (TTSuV2) in swine and many species of Torque teno virus (TTV) in human sera, which were previously tested negative by conventional PCRs.
authors.affiliation: Centre de Recerca en Sanitat Animal, UAB-IRTA, Campus de Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. lisamacera@med.unipi.it
authors.forename: Lisa
authors.initials: L
authors.lastname: Macera
authors.forename: Martí
authors.initials: M
authors.lastname: Cortey
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Maggi
authors.forename: Joaquim
authors.initials: J
authors.lastname: Segalés
authors.forename: Tuija
authors.initials: T
authors.lastname: Kekarainen
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D004266:DNA Virus Infections / Q000175:diagnosis* / Q000662:veterinary* / Q000821:virology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D044967:Serum / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers