title: SAX microscopy with fluorescent nanodiamond probes for high-resolution fluorescence imaging.
abstract: We report the use of fluorescent nanodiamonds (FNDs) as a photostable fluorescent probe for high resolution saturated excitation (SAX) microscopy. We confirmed that FNDs show a nonlinear fluorescence response under saturated excitation conditions generated by intense excitation light. Using FNDs, we quantified the spatial resolution improvement inherent in SAX microscopy, and experimentally demonstrated the scalability of the spatial resolution of SAX microscopy. The photostability of the FNDs allowed us to perform nanoparticle imaging of a multicolor-stained macrophage cell with a spatial resolution beyond the diffraction limit.
authors.forename: Masahito
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Yan-Kai
authors.initials: YK
authors.lastname: Tzeng
authors.forename: Shogo
authors.initials: S
authors.lastname: Kawano
authors.forename: Nicholas I
authors.initials: NI
authors.lastname: Smith
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kawata
authors.forename: Huan-Cheng
authors.initials: HC
authors.lastname: Chang
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Fujita