title: A first case of protease codon 35 amino acid insertion in a HIV-1 subtype B sequence detected in the Bauru region, state of São Paulo, Brazil: case report.
abstract: Amino acid insertions in the protease have rarely been described in HIV-infected patients. One of these insertions has recently been described in codon 35, although its impact on resistance remains unknown. This study presents a case of an HIV variant with an insertion in codon 35 of the protease, described for the first time in Bauru, State of Sao Paulo, Brazil, circulating in a 38-year-old caucasian male with asymptomatic HIV infection since 1997. The variant isolated showed a codon 35 insertion of two amino acids in the protease: a threonine and an aspartic acid, resulting in the amino acid sequence E35E_TD.
authors.affiliation: Laboratório de Biologia Molecular, Divisão Hemocentro, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Botucatu, SP.
authors.forename: Rejane Maria Tommasini
authors.initials: RM
authors.lastname: Grotto
authors.forename: Sílvia Maria
authors.initials: SM
authors.lastname: Corvino
authors.forename: Lilian da Silva Reis
authors.initials: Lda S
authors.lastname: Munhoz
authors.forename: Cibele Gomes
authors.initials: CG
authors.lastname: Ghedini
authors.forename: Maria Inês de Moura Campos
authors.initials: MI
authors.lastname: Pardini
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001938:Brazil; D003062:Codon / Q000235:genetics*; D015658:HIV Infections / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*
chemical_list: D003062:Codon; D016333:HIV Protease