title: Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol.
abstract: Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 μg g(-1) fresh weight, respectively.
authors.affiliation: Equipe de Chimie Analytique des Molécules Bio-Actives, IPHC-DSA, Université de Strasbourg, CNRS, Faculté de Pharmacie 74, route du Rhin, 67400 Illkirch, France.
authors.forename: Eli Adjélé
authors.initials: EA
authors.lastname: Wilson
authors.forename: Saïd
authors.initials: S
authors.lastname: Ennahar
authors.forename: Minjie
authors.initials: M
authors.lastname: Zhao
authors.forename: Martine
authors.initials: M
authors.lastname: Bergaentzle
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
authors.forename: Françoise
authors.initials: F
authors.lastname: Bindler