title: Novel multienzyme oxidative biocatalyst for lignin bioprocessing.
abstract: A novel multienzyme biocatalyst, based on coimmobilization of the laccase and horseradish peroxidase by cross linking and layer-by-layer coating with polyelectrolyte, was designed, synthesized and applied at the development of an oxidative cascade process on lignin. The efficiency and specificity of the new LbL-multienzyme system, the occurrence of a synergy of the co-immobilized enzymes, the lignin oxidation pathway and the nature of the structural modifications occurred in treated lignins have been investigated in the present effort by means of GPC analysis and quantitative (31)P NMR techniques.
authors.affiliation: Dipartimento di Scienze e Tecnologie Chimiche, Tor Vergata University, Via della Ricerca Scientifica, Rome 00133, Italy. crestini@stc.uniroma2.it
authors.forename: Claudia
authors.initials: C
authors.lastname: Crestini
authors.forename: Federica
authors.initials: F
authors.lastname: Melone
authors.forename: Raffaele
authors.initials: R
authors.lastname: Saladino
mesh_terms: D020099:Coated Materials, Biocompatible; D004339:Drug Compounding / Q000379:methods*; D015195:Drug Design; D004795:Enzyme Stability / Q000187:drug effects; D004798:Enzymes / Q000096:biosynthesis* / Q000378:metabolism; D005079:Excipients / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D042845:Laccase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011073:Polyamines / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D020099:Coated Materials, Biocompatible; D004798:Enzymes; D005079:Excipients; D009097:Multienzyme Complexes; D011073:Polyamines; C063994:polyallylamine; D008031:Lignin; D042845:Laccase; D006735:Horseradish Peroxidase