title: In vitro biocompatibility of novel biphasic calcium phosphate-mullite composites.
abstract: In designing new calcium phosphate (CaP)-based composites, the improvement in physical properties (strength, toughness) without compromising the biocompatibility aspect is essential. In a recent study, it has been demonstrated that significant improvement in compressive strength as well as modest enhancement in toughness is achievable in biphasic calcium phosphate (BCP)-based composites with mullite addition (up to 30 wt%). Herein, we report the results of the in vitro cell adhesion, cell proliferation, alkaline phosphatase (ALP) activity, and osteocalcin (OC) production for a series of BCP-mullite (up to 30 wt%) composites. Mouse fibroblast (L929) cell lines were used to examine in vitro cell adhesion and cell proliferation; while osteoblast-like (osteosarcoma, MG63) cells were used for in vitro osteoblastic function study by ALP and OC expression. Much emphasis has been provided to discuss the cell viability and proliferation as well as osteoblastic differentiation marker on the investigated biocomposites in relation to the characteristics of the phase assemblage. On the basis of various observations using multiple biochemical assays, it has been suggested that BCP-mullite composites would be a candidate material for orthopedic applications.
authors.affiliation: Laboratory for Biomaterials, Department Materials Science and Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, UP, India.
authors.forename: Shekhar
authors.initials: S
authors.lastname: Nath
authors.forename: Sushma
authors.initials: S
authors.lastname: Kalmodia
authors.forename: Bikramjit
authors.initials: B
authors.lastname: Basu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D066298:In Vitro Techniques; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology; D015675:Osteocalcin / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D015675:Osteocalcin; C020243:calcium phosphate; D000469:Alkaline Phosphatase