title: Tarsal taste neurons of Helicoverpa assulta (Guenée) respond to sugars and amino acids, suggesting a role in feeding and oviposition.
abstract: Helicoverpa assulta and Helicoverpa armigera are sibling species with different host-plant ranges. We have previously reported electrophysiological and behavioral responses of H.armigera to sugars and amino acids. Here we describe a parallel study performed on H. assulta and compare the results obtained with the two species. In females, fourteen gustatory chemosensilla, identified on one ventrolateral side of the fifth tarsomere were stimulated with sucrose, glucose, fructose, maltose, myo-inositol, and the twenty common amino acids, using the tip-recording technique. The taste receptor neurons in eight chemosensilla were identified sensitive to the sugars, myo-inositol, Lys, Glu, Arg, Trp, and Ser which all induced proboscis extension reflex (PER) when tarsi were stimulated. There was a positive correlation between electrophysiological activities and PER responses triggered by sucrose. No stimulatory effect on oviposition was observed with sugar or amino acid mixtures. In males, three chemosensilla showed responses to the four sugars, but generally weaker than in females. The major difference of the two species was the variety of amino acids triggering electrophysiological responses. The stimulatory effect of sugars and amino acids on H.assulta was also generally weaker than that on H. armigera.
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Zhang
authors.forename: Ling-Qiao
authors.initials: LQ
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.forename: Chen-Zhu
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D002628:Chemoreceptor Cells / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009036:Moths / Q000187:drug effects*; D010058:Oviposition / Q000187:drug effects; D058618:Sensilla / Q000187:drug effects*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates