title: The role of factor XIa (FXIa) catalytic domain exosite residues in substrate catalysis and inhibition by the Kunitz protease inhibitor domain of protease nexin 2.
abstract: To select residues in coagulation factor XIa (FXIa) potentially important for substrate and inhibitor interactions, we examined the crystal structure of the complex between the catalytic domain of FXIa and the Kunitz protease inhibitor (KPI) domain of a physiologically relevant FXIa inhibitor, protease nexin 2 (PN2). Six FXIa catalytic domain residues (Glu(98), Tyr(143), Ile(151), Arg(3704), Lys(192), and Tyr(5901)) were subjected to mutational analysis to investigate the molecular interactions between FXIa and the small synthetic substrate (S-2366), the macromolecular substrate (factor IX (FIX)) and inhibitor PN2KPI. Analysis of all six Ala mutants demonstrated normal K(m) values for S-2366 hydrolysis, indicating normal substrate binding compared with plasma FXIa; however, all except E98A and K192A had impaired values of k(cat) for S-2366 hydrolysis. All six Ala mutants displayed deficient k(cat) values for FIX hydrolysis, and all were inhibited by PN2KPI with normal values of K(i) except for K192A, and Y5901A, which displayed increased values of K(i). The integrity of the S1 binding site residue, Asp(189), utilizing p-aminobenzamidine, was intact for all FXIa mutants. Thus, whereas all six residues are essential for catalysis of the macromolecular substrate (FIX), only four (Tyr(143), Ile(151), Arg(3704), and Tyr(5901)) are important for S-2366 hydrolysis; Glu(98) and Lys(192) are essential for FIX but not S-2366 hydrolysis; and Lys(192) and Tyr(5901) are required for both inhibitor and macromolecular substrate interactions.
authors.affiliation: Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
authors.forename: Ya-Chi
authors.initials: YC
authors.lastname: Su
authors.forename: Tara N
authors.initials: TN
authors.lastname: Miller
authors.forename: Duraiswamy
authors.initials: D
authors.lastname: Navaneetham
authors.forename: Robert T
authors.initials: RT
authors.lastname: Schoonmaker
authors.forename: Dipali
authors.initials: D
authors.lastname: Sinha
authors.forename: Peter N
authors.initials: PN
authors.lastname: Walsh
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D016564:Amyloid beta-Protein Precursor / Q000502:physiology*; D007611:Aprotinin; D002384:Catalysis; D020134:Catalytic Domain; D015945:Factor XIa / Q000037:antagonists & inhibitors* / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D011480:Protease Inhibitors; D017434:Protein Structure, Tertiary
chemical_list: D016564:Amyloid beta-Protein Precursor; D011480:Protease Inhibitors; D007611:Aprotinin; D015945:Factor XIa