title: Albizia lebbeck suppresses histamine signaling by the inhibition of histamine H1 receptor and histidine decarboxylase gene transcriptions.
abstract: Histamine plays major roles in allergic diseases and its action is mediated mainly by histamine H(1) receptor (H1R). We have demonstrated that histamine signaling-related H1R and histidine decarboxylase (HDC) genes are allergic diseases sensitive genes and their expression level affects severity of the allergic symptoms. Therefore, compounds that suppress histamine signaling should be promising candidates as anti-allergic drugs. Here, we investigated the effect of the extract from the bark of Albizia lebbeck (AL), one of the ingredients of Ayruvedic medicines, on H1R and HDC gene expression using toluene-2,4-diisocyanate (TDI) sensitized allergy model rats and HeLa cells expressing endogenous H1R. Administration of the AL extract significantly decreased the numbers of sneezing and nasal rubbing. Pretreatment with the AL extract suppressed TDI-induced H1R and HDC mRNA elevations as well as [(3)H]mepyramine binding, HDC activity, and histamine content in the nasal mucosa. AL extract also suppressed TDI-induced up-regulation of IL-4, IL-5, and IL-13 mRNA. In HeLa cells, AL extract suppressed phorbol-12-myristate-13-acetate- or histamine-induced up-regulation of H1R mRNA. Our data suggest that AL alleviated nasal symptoms by inhibiting histamine signaling in TDI-sensitized rats through suppression of H1R and HDC gene transcriptions. Suppression of Th2-cytokine signaling by AL also suggests that it could affect the histamine-cytokine network.
authors.affiliation: Department of Molecular Pharmacology, Institute of Health-Biosciences, The University of Tokushima Graduate School, Tokushima 770-8505, Japan.
authors.forename: Islam Mohammed
authors.initials: IM
authors.lastname: Nurul
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.forename: Masum
authors.initials: M
authors.lastname: Shahriar
authors.forename: Pichairajan
authors.initials: P
authors.lastname: Venkatesh
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Maeyama
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: Masashi
authors.initials: M
authors.lastname: Hattori
authors.forename: Mohamed Sahabuddin Kabir
authors.initials: MS
authors.lastname: Choudhuri
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takeda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Fukui
mesh_terms: D031289:Albizzia / Q000737:chemistry*; D000818:Animals; D004195:Disease Models, Animal; D006367:HeLa Cells; D006632:Histamine / Q000276:immunology / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000302:isolation & purification / Q000494:pharmacology*; D006640:Histidine Decarboxylase / Q000235:genetics*; D006801:Humans; D008297:Male; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011914:Rats, Inbred BN; D060888:Real-Time Polymerase Chain Reaction; D011969:Receptors, Histamine H1 / Q000276:immunology / Q000378:metabolism*; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D015398:Signal Transduction; D014051:Toluene 2,4-Diisocyanate / Q000276:immunology / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D006634:Histamine H1 Antagonists; D010936:Plant Extracts; D011969:Receptors, Histamine H1; D014051:Toluene 2,4-Diisocyanate; D006632:Histamine; D006640:Histidine Decarboxylase