title: Phage display identifies two Caprine Arthritis Encephalitis Virus env epitopes.
abstract: Using phage display and IgG of a goat infected with Caprine Arthritis Encephalitis Virus (CAEV) we obtained families of 7 mer constrained peptides with consensus motifs LxSDPF/Y and SWN/KHWSY and mapped the epitopes mimicked by them at the Env 6-LISDPY-11 and 67-WNTYHW-72 sites of the mature gp135 amino acid sequence. The first epitope fell into the N-terminal immunogenic aa1-EDYTLISDPYGFS- aa14 site identified previously with a synthetic peptide approach; the second epitope has not been described previously. The first epitope is mostly conserved across CAEV isolates whereas the second newly described epitope is extremely conserved in Small Ruminant Lentiviruses env sequences. As being immunodominant, the epitopes are candidate targets for mimotope-mediated diagnosis and/or neutralization.
authors.affiliation: Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México (UNAM), México D,F,, Ciudad Universitaria, 04510, México. karlen@servidor.unam.mx.
authors.forename: Karlen
authors.initials: K
authors.lastname: Gazarian
authors.forename: Alvaro Aguilar
authors.initials: AA
authors.lastname: Setién
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gazarian
authors.forename: Sebastian Aguilar
authors.initials: SA
authors.lastname: Pierle
mesh_terms: D016088:Arthritis-Encephalitis Virus, Caprine / Q000235:genetics* / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D061505:Cell Surface Display Techniques / Q000662:veterinary; D018604:Epitope Mapping / Q000662:veterinary; D000939:Epitopes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D000939:Epitopes; D014759:Viral Envelope Proteins