title: A bursal pentapeptide (BPP-I), a novel bursal-derived peptide, exhibits antiproliferation of tumor cell and immunomodulator activity.
abstract: The bursa of Fabricius (BF) is the central humoral immune organ unique to birds. Here, we isolated a novel bursal pentapeptide I (BPP-I), LGPGP, from BF. BPP-I could play inhibition effect on MCF-7 but not on CEF or Vero cell proliferation in vitro, and enhance antitumor factor p53 protein expression. Also, BPP-I stimulated antibody production in a dose-dependent manner in hybridoma cell. Furthermore, BPP-I could induce various immune responses in mice immunization experiments, including increase antibody production and cytokines IL-4 and IFN-γ level, and induce T-cell immunophenotyping. These results suggest that BPP-I is a potential immunomodulator of antitumor and immunity. The study could provide some novel insights on the probable candidate reagent for the antitumor and immune improvement.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, 210095, People's Republic of China.
authors.forename: Xiu L
authors.initials: XL
authors.lastname: Feng
authors.forename: Qing T
authors.initials: QT
authors.lastname: Liu
authors.forename: Rui B
authors.initials: RB
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Fang Q
authors.initials: FQ
authors.lastname: Wang
authors.forename: Wen L
authors.initials: WL
authors.lastname: Deng
authors.forename: Ya F
authors.initials: YF
authors.lastname: Qiu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hassan
authors.initials: H
authors.lastname: Ishag
authors.forename: Zhi Y
authors.initials: ZY
authors.lastname: Ma
authors.forename: Qi S
authors.initials: QS
authors.lastname: Zheng
authors.forename: Pu Y
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D002060:Bursa of Fabricius / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology; D007114:Immunization; D053127:Influenza A Virus, H9N2 Subtype / Q000187:drug effects / Q000276:immunology; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D018931:Antineoplastic Agents, Hormonal; D009842:Oligopeptides; D015847:Interleukin-4; D007371:Interferon-gamma