title: Establishment of novel embryonic stem (ES) cell lines from OG2/rtTA blastocysts.
abstract: Embryonic stem (ES) cells derived from the pre-implantation blastocyst-stage embryos have been widely used to investigate the molecular events determining pluripotency and cell lineage differentiation. As the first discovered ES-specific transcription factor, Oct4 has been considered as the core pluripotency factor of ES cells. In the present study, we successfully established seven ES lines from the blastocysts collected from female OG2 (Oct4-GFP transgenic) mice, which have been crossed with male rtTA transgenic mice. The pluripotency of the ES cell lines can be visualized by the expression of Oct4-GFP under fluorescent microscopy and germ-line transmission capability has been further confirmed. More importantly, the presence of rtTA could induce transgene's expression with the help of doxycycline. Therefore, these ES cell lines provide an excellent tool to further discover novel factors affecting pluripotency and to investigate the molecular mechanism of reprogramming in defined transcription factors mediated nuclear reprogramming.
authors.affiliation: College of Life Science, Beijing Normal University, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
authors.forename: Shaorong
authors.initials: S
authors.lastname: Gao
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D002460:Cell Line; D065150:Cellular Reprogramming / Q000235:genetics*; D003433:Crosses, Genetic; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology*; D013724:Teratoma
chemical_list: D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D049452:Green Fluorescent Proteins