title: In vivo and in vitro insulin and fasting control of the transmembrane fatty acid transport proteins in Atlantic salmon (Salmo salar).
abstract: We have examined the nutritional and insulin regulation of the mRNA expression of transmembrane fatty acid (FA) transporters [FA transport protein-1 (FATP1) and CD36] together with the lipoprotein lipase (LPL), the cytosolic FA carrier FA binding protein (FABP3), and mitochondrial FA-CoA and -carnitine palmitoyl transferase carriers (CPT)1 and -2 in Atlantic salmon tissues and myocyte cell culture. Two weeks of fasting diminished FATP1, CD36, and LPL in adipose tissue, suggesting a reduction in FA uptake, while FABP3 increased in liver, probably enhancing the transport of FA to the mitochondria. Insulin injection decreased FATP1 and CD36 in white and red muscles, while both transporters were upregulated in the adipose tissue in agreement with the role of insulin-inhibiting muscle FA oxidation and stimulating adipose fat stores. Serum deprivation of 48 h in Atlantic salmon myotubes increased FATP1, FABP3, and CPT-2, while CPT-1 was diminished. In myotubes, insulin induced FATP1 expression but decreased CD36, FABP3, and LPL, suggesting that FATP1 could be more involved in the insulin-stimulated FA uptake. Insulin increased the FA uptake in myotubes mediated, at least in part, through the relocation of FATP1 protein to the plasma membrane. Overall, Atlantic salmon FA transporters are regulated by fasting and insulin on in vivo and in vitro models.
authors.affiliation: Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain, Av. Diagonal 645, E-08028 Barcelona, Spain. juansangu@gmail.com
authors.forename: Joan
authors.initials: J
authors.lastname: Sánchez-Gurmaches
authors.forename: Tone-Kari
authors.initials: TK
authors.lastname: Østbye
authors.forename: Isabel
authors.initials: I
authors.lastname: Navarro
authors.forename: Jacob
authors.initials: J
authors.lastname: Torgersen
authors.forename: Ernst Morten
authors.initials: EM
authors.lastname: Hevrøy
authors.forename: Bente
authors.initials: B
authors.lastname: Ruyter
authors.forename: Bente E
authors.initials: BE
authors.lastname: Torstensen
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D005215:Fasting / Q000502:physiology*; D050612:Fatty Acid Transport Proteins / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D023421:Models, Animal; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D020317:Salmo salar / Q000502:physiology*
chemical_list: D018955:CD36 Antigens; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D007328:Insulin; D005947:Glucose