title: Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate.
abstract: This study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (DSCBH) as an alternative carbon source to culture Yarrowia lipolytica Po1g for microbial oil and biodiesel production. Sugarcane bagasse hydrolysis with 2.5% HCl resulted in maximum total sugar concentration (21.38 g/L) in which 13.59 g/L is xylose, 3.98 g/L is glucose, and 2.78 g/L is arabinose. Detoxification of SCBH by Ca(OH)₂ neutralization reduced the concentration of 5-hydroxymethylfurfural and furfural by 21.31% and 24.84%, respectively. Growth of Y. lipolytica Po1g in DSCBH with peptone as the nitrogen source gave maximum biomass concentration (11.42 g/L) compared to NH₄NO₃ (6.49 g/L). With peptone as the nitrogen source, DSCBH resulted in better biomass concentration than D-glucose (10.19 g/L), D-xylose (9.89 g/L) and NDSCBH (5.88 g/L). The maximum lipid content, lipid yield and lipid productivity of Y. lipolytica Po1g grown in DSCBH and peptone was 58.5%, 6.68 g/L and 1.76 g/L-day, respectively.
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, 43, Keelung Rd, Sec. 4, Taipei 106, Taiwan, ROC.
authors.forename: Yeshitila Asteraye
authors.initials: YA
authors.lastname: Tsigie
authors.forename: Chun-Yuan
authors.initials: CY
authors.lastname: Wang
authors.forename: Chi-Thanh
authors.initials: CT
authors.lastname: Truong
authors.forename: Yi-Hsu
authors.initials: YH
authors.lastname: Ju
mesh_terms: D001089:Arabinose / Q000378:metabolism; D056804:Biofuels; D018533:Biomass; D002126:Calcium Hydroxide; D002482:Cellulose / Q000737:chemistry*; D002498:Centrifugation; D003470:Culture Media / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006851:Hydrochloric Acid; D006868:Hydrolysis; D008055:Lipids / Q000096:biosynthesis*; D010461:Peptones / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014994:Xylose / Q000378:metabolism; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D008055:Lipids; D010461:Peptones; D002482:Cellulose; C027433:bagasse; D014994:Xylose; D001089:Arabinose; D005947:Glucose; D002126:Calcium Hydroxide; D006851:Hydrochloric Acid