title: Evaluation and optimization of IgY spin column technology in the depletion of abundant proteins from human serum.
abstract: Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.
authors.affiliation: Division of Biology, Anatomy Department, Royal College of Surgeons in Ireland, Dublin, Ireland. lauracorrigan@rcsi.ie
authors.forename: Laura
authors.initials: L
authors.lastname: Corrigan
authors.forename: Caroline
authors.initials: C
authors.lastname: Jefferies
authors.forename: Thomas
authors.initials: T
authors.lastname: Clive Lee
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Daly
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001798:Blood Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; C016512:IgY; D007136:Immunoglobulins