title: Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture.
abstract: Sucrose has several advantages over glucose as a feedstock for bioprocesses, both environmentally and economically. However, most industrial Escherichia coli strains are unable to utilize sucrose. E. coli W can grow on sucrose but stops growing when sucrose concentrations become low. This is undesirable in fed-batch conditions where sugar levels are low between feeding pulses. Sucrose uptake rates were improved by removal of the cscR gene, which encodes a protein that represses expression of the sucrose utilization genes at low sucrose concentrations. Poly-3-hydroxybutyrate (PHB) was used as a model compound in order to assess the effect of improved sugar utilization on bio-production. In the cscR knockout strain, production from sucrose was improved by 50%; this strain also produced 30% more PHB than the wild-type using glucose. This result demonstrates the feasibility of utilizing sucrose as an industrial feedstock for E. coli-based bioprocesses in high cell density culture.
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St. Lucia, QLD 4072, Australia.
authors.forename: Yalun
authors.initials: Y
authors.lastname: Arifin
authors.forename: Suriana
authors.initials: S
authors.lastname: Sabri
authors.forename: Haryadi
authors.initials: H
authors.lastname: Sugiarto
authors.forename: Jens O
authors.initials: JO
authors.lastname: Krömer
authors.forename: Claudia E
authors.initials: CE
authors.lastname: Vickers
authors.forename: Lars K
authors.initials: LK
authors.lastname: Nielsen
mesh_terms: D018533:Biomass; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D006885:Hydroxybutyrates / Q000378:metabolism*; D007218:Industrial Microbiology; D007700:Kinetics; D026901:Membrane Transport Proteins; D011091:Polyesters / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D000094122:Polyhydroxybutyrates
chemical_list: D029968:Escherichia coli Proteins; D006885:Hydroxybutyrates; D026901:Membrane Transport Proteins; D011091:Polyesters; D013395:Sucrose; D014157:Transcription Factors; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C056379:cscB protein, E coli