title: Capillary-composited microfluidic device for heat shock transformation of Escherichia coli.
abstract: This work describes chemical heat shock transformation of foreign plasmid DNA into bacterial host Escherichia coli cells using a capillary-composited microfluidic device. Transformation processes of the loading, mixing, heat shock and recovery of the transformation mixture were carried out automatically in a linear fashion. In addition, by utilizing the capillary with a hollow cylindrical chamber as heating source, simple, low cost local heat shock with accurate heat shock time to transformation mixture was obtained on the microdevice. Results demonstrated that plasmid DNA could be effectively transformed into E. coli, and the transformation efficiency and frequency were as the same level or better than conventional tube-based method. This work complements other microfluidic technologies for potential gene cloning and functional genomics studies.
authors.affiliation: Shaanxi Key Laboratory of Molecular Biology forAgriculture, Colleges of Science and Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Sha
authors.forename: Yaolei
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianchun
authors.initials: J
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Qin
authors.initials: Q
authors.lastname: Tu
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.forename: Xueqin
authors.initials: X
authors.lastname: Wang
authors.forename: Ajing
authors.initials: A
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
mesh_terms: D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010957:Plasmids / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial