title: [Investigation on the natural infectious status of hantaviruses among small mammals in Longquan city, Zhejiang province].
abstract: OBJECTIVE
  To investigate the situation of the natural infection of hantaviruses (HV) in small mammals and to provide evidence for the control and prevention of hemorrhagic fever with renal syndrome (HFRS) in Longquan area, Zhejiang province.
  
  
  METHODS
  Small mammals were captured by night trap, and lung tissue samples were collected and stored in liquid nitrogen. HV antigens were detected by indirect immuno-fluorescence assay (IFA). The partial S genome segment sequences were amplified by RT-PCR. DNAStar program was used for editing and comparing the sequences. Phylogeny was analyzed through PAUP*4.0 software.
  
  
  RESULTS
  319 small animals were collected in Longquan, and 9 hantavirus antigen-positive samples were identified. The positive rate of hantavirus in Apodemus agrarius was 4.97%. Phylogenetic tree constructed by partial S segment (620 - 999 nt) showed that the 9 strains carried by A. agrarius from Longquan all belonged to HTNV, and had a closer evolutionary relationship with isolate Z251 from Zhejiang province.
  
  
  CONCLUSIONS
  Our results indicated that the main host was A. agrarius and the infection rate of HTNV was high in Longquan area.
authors.affiliation: Zhejiang Provincial of Longquan Center for Disease Control and Prevention, Longquan, China.
authors.forename: Miao-ruo
authors.initials: MR
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Xian-dan
authors.initials: XD
authors.lastname: Lin
authors.forename: Sheng-hua
authors.initials: SH
authors.lastname: Mei
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Guo
authors.forename: Yong-zhen
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002681:China; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000662:veterinary* / Q000821:virology; D054884:Host-Pathogen Interactions; D051189:Murinae / Q000821:virology*; D010802:Phylogeny