title: A direct determination of a glucose-arsenic complex by electrospray ionization time of flight mass spectrometry.
abstract: Electrospray ionization time of flight mass spectrometry (ESI-TOF-MS) was used to identify elemental ions from the glucose-arsenic interaction in the aqueous phase. In glucose solution, the most abundant ions were m/z 203, m/z 163, m/z 158, m/z 145 and m/z 115, whereas some additional arsenic bearing ions, m/z 271, m/z 235 and m/z 213 were observed from a glucose-arsenic solution in alkaline pH (≥ 7.5) at 37 °C. The binding was best fitted to 1:1 isotherm model and the value of the dissociation constant (K(d)) was 39.8 μM. Results suggest that the polyatomic ions derived from glucose interact with the available arsenic ions in blood and form a complex which might play a role in diseases caused by arsenic exposure.
authors.affiliation: Mass Spectrometry & Proteomics Lab. Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur 721302, WB, India. sm_crf@yahoo.com
authors.forename: Santi M
authors.initials: SM
authors.lastname: Mandal
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D005947:Glucose; D001151:Arsenic