title: Quantitative analyses of lymphoid tissue in the spleen, lymph nodes and Peyer's patches in cynomolgus monkeys.
abstract: To clarify the morphological characteristics of the cynomolgus monkey immune system, we analyzed quantitative data on their lymphoid organs. Spleens, major lymph nodes and Peyer's patches were sampled from cynomolgus monkeys, and the lymphoid follicle and germinal center areas and percentages of CD3- and CD20-positive areas were calculated. All the organs analyzed showed large interindividual variations in the sizes of lymphoid follicles and germinal centers. Lymphoid follicle in the spleen, submandibular lymph nodes and Peyer's patches showed no marked difference in size. Germinal center size in the mesenteric lymph nodes and Peyer's patches were significantly smaller than those in the spleen. Areas containing T cells were largest in the lymph nodes, while those containing B cells were largest in the spleen and Peyer's patches. The mean size of the splenic lymphoid follicle in cynomolgus monkeys is larger than that in rats and similar to that in humans. Based on the large individual variation and the characteristics of lymphoid organs, it is important to use cynomolgus monkeys in standard toxicity studies. Taking advantage of the characteristics of each species enables reliable evaluation of the immunologic system in standard toxicity studies.
authors.affiliation: Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories, Ltd, 2438 Miyanoura, Kagoshima 891–1394, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Moriyama
authors.forename: Junko
authors.initials: J
authors.lastname: Fujishima
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Furukawa
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Rinya
authors.initials: R
authors.lastname: Kodama
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Takaharu
authors.initials: T
authors.lastname: Nagaoka
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kamimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Takuya
authors.initials: T
authors.lastname: Hirai
authors.forename: Ryoji
authors.initials: R
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D018951:Antigens, CD20 / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D005260:Female; D008198:Lymph Nodes / Q000033:anatomy & histology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000033:anatomy & histology / Q000378:metabolism; D008252:Macaca fascicularis / Q000033:anatomy & histology / Q000276:immunology* / Q000378:metabolism; D008297:Male; D009929:Organ Size; D010581:Peyer's Patches / Q000033:anatomy & histology / Q000378:metabolism; D013154:Spleen / Q000033:anatomy & histology / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D018951:Antigens, CD20; D017252:CD3 Complex