title: Interrogating the repertoire: broadening the scope of peptide-MHC multimer analysis.
abstract: Labelling antigen-specific T cells with peptide-MHC multimers has provided an invaluable way to monitor T cell-mediated immune responses. A number of recent developments in this technology have made these multimers much easier to make and use in large numbers. Furthermore, enrichment techniques have provided a greatly increased sensitivity that allows the analysis of the naive T cell repertoire directly. Thus, we can expect a flood of new information to emerge in the coming years.
authors.affiliation: Department of Microbiology and Immunology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA. mmdavis@stanford.edu
authors.forename: Mark M
authors.initials: MM
authors.lastname: Davis
authors.forename: John D
authors.initials: JD
authors.lastname: Altman
authors.forename: Evan W
authors.initials: EW
authors.lastname: Newell
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology; D058948:Cell Tracking / Q000379:methods; D003141:Communicable Diseases / Q000276:immunology; D018604:Epitope Mapping / Q000379:methods / Q000639:trends; D018984:Epitopes, T-Lymphocyte / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques / Q000639:trends; D016219:Immunotherapy, Adoptive / Q000379:methods; D000922:Immunotoxins / Q000276:immunology / Q000627:therapeutic use; D008285:Major Histocompatibility Complex / Q000276:immunology*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D010455:Peptides / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D018984:Epitopes, T-Lymphocyte; D000922:Immunotoxins; D010455:Peptides; D011948:Receptors, Antigen, T-Cell; D014612:Vaccines