title: Preparative isolation, fast centrifugal partition chromatography purification and biological activity of cajaflavanone from Derris ferruginea stems.
abstract: BACKGROUND
  The Derris genus is known to contain flavonoid derivatives, including prenylated flavanones and isoflavonoids such as rotenoids, which are generally associated with significant biological activity.
  
  
  OBJECTIVE
  To develop an efficient preparative isolation procedure for bioactive cajaflavanone.
  
  
  METHODS
  Fast centrifugal partition chromatography (FCPC) was optimised to purify cajaflavanone from Derris ferruginea stems in a single step as compared to fractionation from the cyclohexane extract by successive conventional solid-liquid chromatography procedures. The purification yield, purity, time and solvent consumption per procedure are described. The anti-fungal, anti-bacterial, anti-leishmanial, anti-plasmodial, anti-oxidant activities and the inhibition of advanced glycation end-products (AGEs) by cajaflavanone accumulation are described.
  
  
  RESULTS
  FCPC enabled cajaflavanone purification in a single separation step, yielding sufficient quantities to perform in vitro biological screening. Interestingly, cajaflavanone had an inhibitory effect on the formation of AGEs, without displaying any in vitro anti-oxidant activity.
  
  
  CONCLUSIONS
  A simple and efficient procedure, in comparison with other preparative methods, for bioactive cajaflavone purification has been developed using FCPC.
authors.affiliation: SONAS EA 921, IFR 149, Quasav UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 16 Bd Daviers, 49100 Angers, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Morel
authors.forename: Anne
authors.initials: A
authors.lastname: Landreau
authors.forename: Van Hung
authors.initials: VH
authors.lastname: Nguyen
authors.forename: Séverine
authors.initials: S
authors.lastname: Derbré
authors.forename: Philippe
authors.initials: P
authors.lastname: Grellier
authors.forename: Patrice Le
authors.initials: PL
authors.lastname: Pape
authors.forename: Fabrice
authors.initials: F
authors.lastname: Pagniez
authors.forename: Marc
authors.initials: M
authors.lastname: Litaudon
authors.forename: Pascal
authors.initials: P
authors.lastname: Richomme
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology; D001232:Aspergillus fumigatus / Q000187:drug effects / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D041221:Candida glabrata / Q000187:drug effects / Q000254:growth & development; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002845:Chromatography / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry; D029866:Derris / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D044950:Flavanones / Q000302:isolation & purification* / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D018320:Leishmania major / Q000187:drug effects / Q000254:growth & development; D010963:Plasmodium falciparum / Q000187:drug effects / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000981:Antiprotozoal Agents; D003510:Cyclohexanes; D044950:Flavanones; D017127:Glycation End Products, Advanced; C506365:Cyclohexane; C028610:flavanone