title: [The connection between HLA microsatellites and HLA-B*27 gene in patients with psoriatic arthritis in Croatian population].
abstract: The polymorphism at HLA microsatellites (D6S248, D6S2674, D6S2811 and D6S273) among patients with psoriatic arthritis (PsA) (N = 22) and healthy control subjects (K; N = 94), as well as haplotypic associations between tested loci were analysed in this study. All subjects were previously typed for HLA-A and -B by PCR-SSP method and were HLA-B*27 positive. HLA microsatellites were analysed using PCR-STR method and electrophoresis in an ALFexpress sequencer. The results demonstrated statistically significant P value for following alleles: D6S273-3 (PsA-21.1% vs. K-4.9%; P = 0.0013) and D6S273-4 (PsA-15.8% vs. K-32.1%; P = 0.0180). Analysis of haplotypic associations showed the only statistically significant difference for combination HLA-B*27/D6S273-4 (PsA-10.5% vs. K-40.2%; P = 0.0164). The results presented in this study lead to the assumption that some other gene/s involved in ethiology of PsA are located in the proximity of D6S273 microsatellite, but in order to reach a final conclusion, an increase in the number of patients is necessary.
authors.affiliation: Zavod za tipizaciju tkiva, Klinicki zavod za laboratorijsku dijagnostiku, Klinicki bolnicki centar Zagreb, Kispatićeva 12, 10000 Zagreb.
authors.forename: Davor
authors.initials: D
authors.lastname: Stimac
authors.forename: Zorana
authors.initials: Z
authors.lastname: Grubić
authors.forename: Katarina
authors.initials: K
authors.lastname: Stingli
authors.forename: Porin
authors.initials: P
authors.lastname: Perić
authors.forename: Bozidar
authors.initials: B
authors.lastname: Curković
authors.forename: Renata
authors.initials: R
authors.lastname: Zunec
mesh_terms: D000328:Adult; D000368:Aged; D015535:Arthritis, Psoriatic / Q000235:genetics*; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged
chemical_list: D006680:HLA Antigens; D015796:HLA-B27 Antigen