title: Non-digestible fraction of cooked bean (Phaseolus vulgaris L.) cultivar Bayo Madero suppresses colonic aberrant crypt foci in azoxymethane-induced rats.
abstract: The non-digestible fraction (NDF) of common bean (Phaseolus vulgaris L.) cultivar Bayo Madero was evaluated for its chemopreventive effect on azoxymethane (AOM) induced aberrant crypt foci (ACF) in rats. Diets containing cooked beans (CB) or its non-digestible fraction (NDF) were fed to 72 male rats after 2 azoxymethane injections (15 mg kg(-1) of body weight once a week for 2 weeks). ACF number, short chain fatty acids (SCFA) and β-glucuronidase activity were measured in colon sections from rats sacrificed 7 weeks after the last AOM injection. Food intake and weight gain of rats were unaffected by CB and NDF. CB and NDF suppressed the AOM-induced formation of ACF (0.8 and 1.5 ACF/distal zone, respectively vs. 6.6 ACF/distal zone based on methylene blue stain) and lowered β-glucuronidase activity in cecal, colonic and fecal content compared to AOM group. SCFA production was not significantly different among fecal, cecal and colonic content. These results indicate that CB and NDF from Bayo Madero provide direct chemoprotection against early stage of azoxymethane (AOM)-induced colon cancer in rats.
authors.affiliation: Programa de Posgrado en Alimentos del Centro de la República (PROPAC), Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Querétaro, Qro 76010, México.
authors.forename: Haydé Azeneth
authors.initials: HA
authors.lastname: Vergara-Castañeda
authors.forename: Ramón Gerardo
authors.initials: RG
authors.lastname: Guevara-González
authors.forename: Minerva
authors.initials: M
authors.lastname: Ramos-Gómez
authors.forename: Rosalía
authors.initials: R
authors.lastname: Reynoso-Camacho
authors.forename: Horacio
authors.initials: H
authors.lastname: Guzmán-Maldonado
authors.forename: Ana Angélica
authors.initials: AA
authors.lastname: Feregrino-Pérez
authors.forename: B Dave
authors.initials: BD
authors.lastname: Oomah
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Loarca-Piña
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001397:Azoxymethane / Q000633:toxicity*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D003296:Cooking / Q000379:methods*; D019610:Cytoprotection / Q000187:drug effects*; D004040:Dietary Carbohydrates / Q000494:pharmacology; D004043:Dietary Fiber / Q000494:pharmacology; D004063:Digestion / Q000502:physiology; D004195:Disease Models, Animal; D004435:Eating / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000494:pharmacology; D005243:Feces / Q000737:chemistry; D007413:Intestinal Mucosa / Q000187:drug effects / Q000473:pathology; D008297:Male; D027805:Phaseolus / Q000737:chemistry* / Q000145:classification; D014674:Plant Proteins, Dietary / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility
chemical_list: D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D014674:Plant Proteins, Dietary; D001397:Azoxymethane