title: Oxygen consumption is a quality marker for human oocyte competence conditioned by ovarian stimulation regimens.
abstract: OBJECTIVE
  To evaluate the effect of different ovarian stimulation protocols on oocyte respiration and to investigate the relationship between oocyte oxygen consumption and reproductive outcome.
  
  
  METHODS
  Prospective observational cohort study.
  
  
  METHODS
  Infertility clinic in a university hospital.
  
  
  METHODS
  A total of 349 oocytes from 56 IVF treatment cycles in our oocyte donation program.
  
  
  METHODS
  None.
  
  
  METHODS
  Average oocyte oxygen consumption rate in fmol/s. We correlated oxygen consumption values with ovarian stimulation features, fertilization, embryo quality on days 2 and 3, and implantation.
  
  
  RESULTS
  Differences in the measured oxygen consumption rates were found depending on which type of gonadotropins were used in the stimulation protocol. Higher consumption rates were found for oocytes that underwent normal fertilization compared with rates from nonfertilized or abnormal oocytes (odds ratio = 1.340; 95% confidence intervals = 1.037-1.732). Furthermore, higher oxygen consumption was observed for those oocytes which generated embryos that implanted compared with those that did not implant (6.21 ± 0.849 fmol/s vs. 5.23 ± 0.345 fmol/s.
  
  
  CONCLUSIONS
  Measurement of oxygen consumption rates for individual oocytes before fertilization provides a noninvasive marker of oocyte quality and hence a quantitative assessment of the reproductive potential for the oocyte.
authors.affiliation: Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain.
authors.forename: Aberto
authors.initials: A
authors.lastname: Tejera
authors.forename: Javier
authors.initials: J
authors.lastname: Herrero
authors.forename: M J
authors.initials: MJ
authors.lastname: de Los Santos
authors.forename: Nicolás
authors.initials: N
authors.lastname: Garrido
authors.forename: Niels
authors.initials: N
authors.lastname: Ramsing
authors.forename: Marcos
authors.initials: M
authors.lastname: Meseguer
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000378:metabolism; D010064:Embryo Implantation / Q000502:physiology*; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005306:Fertilization / Q000502:physiology; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D018587:Oocyte Donation; D009865:Oocytes / Q000378:metabolism*; D010062:Ovulation Induction / Q000379:methods*; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods
chemical_list: D015415:Biomarkers; D010100:Oxygen