title: Mycobacterium tuberculosislpdC, Rv0462, induces dendritic cell maturation and Th1 polarization.
abstract: Mycobacterium tuberculosis, the etiological factor of pulmonary tuberculosis, causes significant morbidity and mortality worldwide. Activation of host immune responses for containment of mycobacterial infections involves participation of innate immune cells, such as dendritic cells (DCs). In this study, we demonstrated that the gene encoding lipoamide dehydrogenase C (lpdC) from M. tuberculosis, Rv0462, induce maturation and activation of DCs involved in the MAPKs signaling pathway. Moreover, Rv0462-treated DCs activated naïve T cells, polarized CD4(+) and CD8(+) T cells to secrete IFN-γ in syngeneic mixed lymphocyte reactions, which would be expected to contribute to Th1 polarization of the immune response. Our results suggest that Rv0462 can contribute to the innate and adaptive immune responses during tuberculosis infection, and thus modulate the clinical course of tuberculosis.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Pusan National University, Beom-eo Ri, Mulgum Eop, Yangsan, Gyeongsangnam-do 626-770, South Korea.
authors.forename: Deok Rim
authors.initials: DR
authors.lastname: Heo
authors.forename: Sung Jae
authors.initials: SJ
authors.lastname: Shin
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Kim
authors.forename: Kyung Tae
authors.initials: KT
authors.lastname: Noh
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Park
authors.forename: Kwang Hee
authors.initials: KH
authors.lastname: Son
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.forename: Min-Goo
authors.initials: MG
authors.lastname: Lee
authors.forename: Daejin
authors.initials: D
authors.lastname: Kim
authors.forename: Yong Kyoo
authors.initials: YK
authors.lastname: Shin
authors.forename: In Duk
authors.initials: ID
authors.lastname: Jung
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D016764:Cell Polarity / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D018664:Interleukin-12 / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D014376:Tuberculosis / Q000378:metabolism* / Q000382:microbiology
chemical_list: D018664:Interleukin-12; D008058:Dihydrolipoamide Dehydrogenase