title: Individual variation in growth factor concentrations in platelet-rich plasma and its influence on human mesenchymal stem cells.
abstract: BACKGROUND
  The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal stem cells (HMSCs).
  
  
  METHODS
  The concentrations of 7 representative GFs in activated PRP (aPRP) were measured using ELISA. The effects of PRP on the proliferation and alkaline phosphatase (ALP) activity of HMSCs were examined using several concentrations of aPRP from 3 donors; the relationships between the GF levels and these biologic effects were then evaluated using 10% aPRP from 5 subgroups derived from 39 total donors. HMSCs were cultured in DMEM with the addition of aPRP for 4 or 12 days; then, DNA content and ALP activity were measured.
  
  
  RESULTS
  The quantity of DNA increased significantly at a 10% concentration of aPRP, but the ALP activity was suppressed at this concentration of aPRP. The GF concentrations varied among donors, and 5 subgroups of characteristic GF release patterns were identified via cluster analysis. DNA levels differed significantly between groups and tended to be higher in groups with higher concentrations of transforming growth factor-beta1 (TGF-β1) and platelet-derived growth factors (PDGFs). DNA quantity was positively correlated with TGF-β1 concentration, and was negatively correlated with donor age. ALP activity was negatively correlated with PDGF-BB concentration.
  
  
  CONCLUSIONS
  The varying GF concentrations may result in different biologic effects; thus, individual differences in GF levels should be considered for reliable interpretation of the biologic functions and standardized application of PRP.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
authors.forename: Hee Soon
authors.initials: HS
authors.lastname: Cho
authors.forename: In Hwan
authors.initials: IH
authors.lastname: Song
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Min Cheol
authors.initials: MC
authors.lastname: Sung
authors.forename: Myun-Whan
authors.initials: MW
authors.lastname: Ahn
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Song
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000077214:Becaplermin; D001782:Blood Donors; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D004247:DNA / Q000032:analysis; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin; D004247:DNA; D000469:Alkaline Phosphatase