title: Cytotoxicity evaluation of Gutta Flow and Endo Sequence BC sealers.
abstract: OBJECTIVE
  This study evaluated the cytotoxicity of GuttaFlow and EndoSequence BC sealers and compared them with AH Plus and Tubli-Seal sealers.
  
  
  METHODS
  Samples (0.5 mg) of freshly mixed or set BC, GuttaFlow, AH Plus, and Tubli-Seal sealers were eluted with 300, 600, and 1,000 μL cell culture medium for 24 and 72 hours. L929 cells were seeded into 96-well plates at 3 × 10(4) cells/well and cultured with 100 μL eluate from each eluate group. Cells cultured only with culture medium served as control. After 24 hours' incubation, the cytotoxicity was evaluated by MTT assay. Cell viability was calculated as the percentage of the control group, and the results were analyzed with a one-way analysis of variance.
  
  
  RESULTS
  For the freshly mixed sealer, cell viability in the AH Plus group was less than in all of the other 3 sealer groups. The Tubli-Seal sealer group had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. For the set sealer, the Tubli-Seal and AH Plus groups had less cell viability than the EndoSequence BC and GuttaFlow sealer groups. There was no cell viability difference between the EndoSequence BC and GuttaFlow sealer groups in the either freshly mixed or set sealer group.
  
  
  CONCLUSIONS
  The GuttaFlow and EndoSequence BC sealers have lower cytotoxicity than the AH Plus and Tubli-Seal sealers.
authors.affiliation: Division of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut 06030-1715, USA.
authors.forename: Keivan
authors.initials: K
authors.lastname: Zoufan
authors.forename: Jin
authors.initials: J
authors.lastname: Jiang
authors.forename: Takashi
authors.initials: T
authors.lastname: Komabayashi
authors.forename: Yu-Hsiung
authors.initials: YH
authors.lastname: Wang
authors.forename: Kamran E
authors.initials: KE
authors.lastname: Safavi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000633:toxicity; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000494:pharmacology; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D006171:Gutta-Percha / Q000737:chemistry / Q000633:toxicity; D051379:Mice; D010087:Oxides / Q000737:chemistry / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D017640:Silicates / Q000737:chemistry / Q000633:toxicity; D013997:Time Factors; D015035:Zinc Oxide-Eugenol Cement / Q000737:chemistry / Q000633:toxicity
chemical_list: D002130:Calcium Phosphates; D017077:Culture Media, Conditioned; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; C550603:EndoSequence root repair material; D004853:Epoxy Resins; C515718:GuttaFlow; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C534916:epoxy resin-based root canal sealer; C048769:tubliseal; D006171:Gutta-Percha