title: Study of DNA interactions with cyclic chalcone derivatives by spectroscopic techniques.
abstract: A series of chalcone derivatives (1-4) were studied. The interaction between these ligands and calf thymus DNA was studied with UV-vis spectrophotometry, fluorescence and circular dichroism spectroscopy. The binding constants K were estimated at 0.5-4.6×10(5) M(-1). All these measurements indicated that the compounds behave as effective DNA-intercalating agents. Electrophoretic separation proved that ligands inhibited topoisomerase I at a concentration of 60 μM.
authors.affiliation: Department of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Safarik University in Košice, Trieda SNP 1, Košice 040 66, Slovak Republic. stefanisinova@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Štefanišinová
authors.forename: V
authors.initials: V
authors.lastname: Tomečková
authors.forename: M
authors.initials: M
authors.lastname: Kožurková
authors.forename: A
authors.initials: A
authors.lastname: Ostró
authors.forename: M
authors.initials: M
authors.lastname: Mareková
mesh_terms: D000818:Animals; D002417:Cattle; D002599:Chalcone / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism* / Q000494:pharmacology; D004347:Drug Interactions; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D007364:Intercalating Agents / Q000494:pharmacology*; D008954:Models, Biological; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D006571:Heterocyclic Compounds; D007364:Intercalating Agents; D002599:Chalcone; D004247:DNA; C427203:calf thymus DNA