title: A plasma metabonomic investigation into the intervention of volatile oil of Magnolia biondii Pamp on rat model of acute inflammation.
abstract: BACKGROUND
  The dried flower buds of Magnolia biondii Pamp (Magnoliaceae) possesses significant anti-inflammatory activities.
  
  
  OBJECTIVE
  Volatile oil in Magnolia biondii Pamp (VOMbP) is considered to be important pharmacologically active individuals against acute inflammation, but its exact anti-inflammatory mechanism remains elusive. In this study, we aimed to investigate the intervention of VOMbP on rats with acute inflammation and explore the possible anti-inflammatory mechanisms of VOMbP with metabonomic strategy.
  
  
  METHODS
  Acute inflammation was induced by subcutaneously injection of carrageenan in the rats. Plasma was analyzed using gas chromatography-mass spectrometry (GC-MS), based on which the principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA) models were established for metabonomic analysis.
  
  
  RESULTS
  It was revealed that the pretreatment of VOMbP in acute inflammatory rats induces a substantial and characteristic change in their metabolic profiles. Some significantly changed metabolites, including hexadecanoic acid, linoleic acid, oleic acid, stearic acid, and cholesterol, were found to be reasonable in explaining the anti-inflammatory mechanism of VOMbP.
  
  
  CONCLUSIONS
  In all, it is likely that VOMbP intervenes the metabolic process of inflammatory rats by affecting the fatty acid and cholesterol metabolism. Our work also indicated that the metabonomics method is a promising tool for performing intervention and mechanism research of traditional Chinese medicines.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Qi
authors.forename: Liping
authors.initials: L
authors.lastname: Qu
authors.forename: Yutian
authors.initials: Y
authors.lastname: Wu
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D002784:Cholesterol / Q000097:blood*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000097:blood*; D035264:Flowers; D008401:Gas Chromatography-Mass Spectrometry; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D016018:Least-Squares Analysis; D050356:Lipid Metabolism / Q000187:drug effects*; D031566:Magnolia / Q000737:chemistry; D008297:Male; D008516:Medicine, Chinese Traditional; D055432:Metabolomics / Q000379:methods; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D005227:Fatty Acids; D009822:Oils, Volatile; D010938:Plant Oils; D002351:Carrageenan; D002784:Cholesterol