title: High throughput quantitative molecular lipidomics.
abstract: Applications in biomedical research increasingly demand detailed lipid molecule information acquired at high throughput. Although the recent advances in lipidomics offer to delineate the lipidomes in detail, the challenge remains in performing such analyses at the requested quality and to maintain the quality also in a high throughput setting. In this review we describe a high throughput molecular lipidomic solution based on robotic assisted sample preparation and lipid extraction and multiple lipidomic platforms integrated with a sophisticated bioinformatics system. As demonstrated, the virtue of this lipidomic toolkit lies in its high throughput delivery of comprehensive quantitative lipidomic outputs at the molecular lipid level, its ease of scalability and its capability to serve in a regulatory setting. We anticipate that this toolkit will contribute to basic research, nutritional research and promote the discovery of new disease biomarkers, disease related mechanisms of actions and drug targets.
authors.affiliation: Zora Biosciences, Espoo, Finland.
authors.forename: Hye R
authors.initials: HR
authors.lastname: Jung
authors.forename: Tuulia
authors.initials: T
authors.lastname: Sylvänne
authors.forename: Kaisa M
authors.initials: KM
authors.lastname: Koistinen
authors.forename: Kirill
authors.initials: K
authors.lastname: Tarasov
authors.forename: Dimple
authors.initials: D
authors.lastname: Kauhanen
authors.forename: Kim
authors.initials: K
authors.lastname: Ekroos
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D001331:Automation; D019295:Computational Biology; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis* / Q000737:chemistry
chemical_list: D008055:Lipids