title: Automatic abdominal fat assessment in obese mice using a segmental shape model.
abstract: OBJECTIVE
  To develop a computerized image analysis method to assess the quantity and distribution of abdominal fat tissues in an obese (ob/ob) mouse model relevant to 7 T magnetic resonance imaging (MRI).
  
  
  METHODS
  A novel segmental shape model is presented that separates visceral adipose tissue (VAT) from subcutaneous adipose tissue (SAT). With shape and distance constraints, it deforms a contour inwards from the skin to the muscle wall and separates the connecting adipose tissues in an ob/ob mouse. The fat tissues are segmented by the adaptive fuzzy C means method to compensate for intensity variation in adipose images. The results were obtained by logical operations applied on the extracted fat images and the separated adipose masks.
  
  
  RESULTS
  The method was validated by manual segmentations on 109 axial slice images from 7 ob/ob mice. The average correlation coefficients of measured sizes between the automatic and manual results for total adipose tissue (TAT) is 0.907; SAT is 0.944; VAT is 0. 950. The average Dice coefficient of their positions for TAT is 0.941, SAT is 0.935, and VAT is 0.920.
  
  
  CONCLUSIONS
  The automated results correlate well with manual segmentations and the method can be used to increase laboratory automation.
authors.affiliation: Department of Radiology, Children's Hospital Los Angeles, University of Southern California, Los Angeles, California 90027, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Tang
authors.forename: Priyank
authors.initials: P
authors.lastname: Sharma
authors.forename: Marvin D
authors.initials: MD
authors.lastname: Nelson
authors.forename: Richard
authors.initials: R
authors.lastname: Simerly
authors.forename: Rex A
authors.initials: RA
authors.lastname: Moats
mesh_terms: D050153:Abdominal Fat / Q000033:anatomy & histology*; D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001331:Automation; D001823:Body Composition; D005260:Female; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D023421:Models, Animal; D012016:Reference Values; D015203:Reproducibility of Results