title: Application of hybrid linear ion trap-high resolution mass spectrometry to the analysis of mycotoxins in beer.
abstract: This paper reports the application of liquid chromatography electrospray ionization ion trap-orbitrap mass spectrometry for the determination of 18 mycotoxins (aflatoxins, fumonisins, trichothecenes, ochratoxin A, sterogmatocystin, beauvaricin, zearalenone and zearalenol) in beer. The extraction procedure was carried out by solid phase extraction (SPE): SPE columns were conditioned with acetonitrile/methanol and water. Beer was loaded onto the column which was washed with water. In these conditions, the recoveries were more than 65% and the relative standard deviation (RSD) were below 18%. The lowest limits of quantification (LLOQ) ranged from 9 to 155 ng ml(-1). Matrix-matched calibration was performed for each beer and reliable results were obtained from selected mycotoxins. The method was applied to the analysis of 25 commercial beers. Taking advantage of the hybrid capabilities, the presence of other mycotoxins were checked; enniatins (A, A(1), B and B(1)) and fusaproliferin were studied in all the tested samples. The survey detected the presence of zearalenone in one stout beer sample.
authors.affiliation: Department de Medicina Preventiva I Salut Pública, Facultat de Farmàcia, Universitat de València, Burjassot, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Rubert
authors.forename: J
authors.initials: J
authors.lastname: Mañes
authors.forename: K J
authors.initials: KJ
authors.lastname: James
authors.forename: C
authors.initials: C
authors.lastname: Soler
mesh_terms: D001515:Beer / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
chemical_list: D009183:Mycotoxins