title: Arabidopsis CSLD1 and CSLD4 are required for cellulose deposition and normal growth of pollen tubes.
abstract: The cell wall is important for pollen tube growth, but little is known about the molecular mechanism that controls cell wall deposition in pollen tubes. Here, the functional characterization of the pollen-expressed Arabidopsis cellulose synthase-like D genes CSLD1 and CSLD4 that are required for pollen tube growth is reported. Both CSLD1 and CSLD4 are highly expressed in mature pollen grains and pollen tubes. The CSLD1 and CSLD4 proteins are located in the Golgi apparatus and transported to the plasma membrane of the tip region of growing pollen tubes, where cellulose is actively synthesized. Mutations in CSLD1 and CSLD4 caused a significant reduction in cellulose deposition in the pollen tube wall and a remarkable disorganization of the pollen tube wall layers, which disrupted the genetic transmission of the male gametophyte. In csld1 and csld4 single mutants and in the csld1 csld4 double mutant, all the mutant pollen tubes exhibited similar phenotypes: the pollen tubes grew extremely abnormally both in vitro and in vivo, which indicates that CSLD1 and CSLD4 are not functionally redundant. Taken together, these results suggest that CSLD1 and CSLD4 play important roles in pollen tube growth, probably through participation in cellulose synthesis of the pollen tube wall.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Guangyan
authors.initials: G
authors.lastname: Xiong
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Tan
authors.forename: Ke-Zhen
authors.initials: KZ
authors.lastname: Yang
authors.forename: Zi-Chen
authors.initials: ZC
authors.lastname: Wang
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhou
authors.forename: De
authors.initials: D
authors.lastname: Ye
authors.forename: Li-Qun
authors.initials: LQ
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000235:genetics / Q000378:metabolism; D053205:Pollen Tube / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002482:Cellulose; C569924:CSLD1 protein, Arabidopsis; C569925:CSLD4 protein, Arabidopsis; D005964:Glucosyltransferases