title: Molecular detection of Anaplasma phagocytophilum and Borrelia burgdorferi in Ixodes ricinus ticks from Istanbul metropolitan area and rural Trakya (Thrace) region of north-western Turkey.
abstract: We demonstrated the presence of the agent of human granulocytic anaplasmosis (HGA), Anaplasma phagocytophilum, and the agent of Lyme borreliosis, Borrelia burgdorferi sensu lato, in north-western Turkey. A total of 241 questing Ixodes ricinus adult ticks were sampled by flagging from recreational parks of the Asiatic and European sides of the heavily populated Istanbul metropolitan area and rural forests of Kirklareli located in the Thrace region in 2008. Both tick-borne pathogens were detected and identified by PCR and DNA sequencing analysis. A. phagocytophilum infection rates were 2.7% in Istanbul and 17.5% in the Kirklareli area. B. burgdorferi sensu lato infection rates were 38.7% in Istanbul and 11.4% in the Kirklareli area. Only 3 of 241 ticks were coinfected with A. phagocytophilum and B. burgdorferi sensu lato.
authors.affiliation: Department of Biology, Faculty of Science, Division of Microbiology, Trakya University, Balkan Campus, Edirne, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Sen
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Uchishima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Fukui
authors.forename: Teruki
authors.initials: T
authors.lastname: Kadosaka
authors.forename: Norio
authors.initials: N
authors.lastname: Ohashi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Masuzawa
mesh_terms: D041081:Anaplasma phagocytophilum / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D025065:Borrelia burgdorferi / Q000235:genetics / Q000302:isolation & purification*; D018884:Ixodes / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014421:Turkey
chemical_list: D012336:RNA, Ribosomal, 16S