title: Hepatitis B virus large surface protein in serum as a candidate biomarker for evaluating hepatitis B virus infection.
abstract: OBJECTIVE
  To develop a sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of serum hepatitis B virus large surface protein (HBV-LP), and study the clinical value of HBV-LP.
  
  
  METHODS
  Serum HBV-LP levels and a panel of other HBV markers were investigated in a large population of patients with chronic HBV. The clinical value of HBV-LP was evaluated by comparing the coincidence of detection of HBV markers and the change of serum HBV-LP level during antiviral therapy.
  
  
  RESULTS
  The ELISA was found to be sensitive and specific for the detection of HBV-LP. Serum HBV-LP level was positively correlated with HBV DNA (r=0.743) in HBV patients. Among the five HBV markers tested, HBV-LP displayed the highest coincidence rate (94.7%) with HBV DNA.
  
  
  CONCLUSIONS
  Serum HBV-LP was strongly correlated with HBV DNA. This ELISA therefore offers a promising approach for the diagnosis and treatment monitoring of HBV patients.
authors.affiliation: Department of Clinical Laboratory, Second Affiliated Hospital of Southeast University, Nanjing, China.
authors.forename: Nian-Yue
authors.initials: NY
authors.lastname: Wang
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Bo-An
authors.initials: BA
authors.lastname: Li
authors.forename: Chang-Qin
authors.initials: CQ
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000188:drug therapy* / Q000821:virology*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000188:drug therapy / Q000821:virology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012680:Sensitivity and Specificity; D044967:Serum / Q000821:virology; D014759:Viral Envelope Proteins / Q000097:blood* / Q000276:immunology; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C096362:L protein, hepatitis B virus; D014759:Viral Envelope Proteins