title: Oligonucleotide-functionalized gold nanoparticles-enhanced QCM-D sensor for mercury(II) ions with high sensitivity and tunable dynamic range.
abstract: A quartz crystal microbalance with dissipation monitoring (QCM-D) sensor was developed for highly sensitive and specific detection of mercury(II) ions (Hg(2+)) with a tunable dynamic range, using oligonucleotide-functionalized gold nanoparticles (GNPs) for both frequency and dissipation amplification. The fabrication of the sensor employed a 'sandwich-type' strategy, and formation of T-Hg(2+)-T structures in linker DNA reduced the hybridization of the GNPs-tagged DNA on the gold electrode, which could be used as the molecular switch for Hg(2+) sensing. This QCM-D mercury sensor showed a linear response of 10-200 nM, with detection limits of 4 nM and 7 nM for frequency and dissipation measurements, respectively. Moreover, the dynamic range of the sensor could be tuned by simply altering the concentration of linker DNA without designing new sensors in the cases where detection of Hg(2+) at different levels is required. This sensor afforded excellent selectivity toward Hg(2+) compared with other potential coexisting metal ions. The feasibility of the sensor was demonstrated by analyzing Hg(2+)-spiked tap- and lake-water samples with satisfactory recoveries. The proposed approach extended the application of the QCM-D system in metal ions sensing, and could be adopted for the detection of other analytes when complemented with the use of functional DNA structures.
authors.affiliation: Beijing National Laboratory for Molecular Science, Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Wu
authors.forename: Dingzhong
authors.initials: D
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D005618:Fresh Water / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D007477:Ions; D009841:Oligonucleotides; D014874:Water Pollutants, Chemical; D006046:Gold; D008628:Mercury