title: Improved biocompatibility of novel poly(L-lactic acid)/β-tricalcium phosphate scaffolds prepared by an organic solvent-free method.
abstract: A porous poly(L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously interconnected porous structure with a porosity of 70% and compressive strength of 1.35 MPa. The methylthiazol tetrazolium values and alkaline phosphatase (ALP) activity of osteoblasts seeded on the solvent-free scaffold were significant higher than those of the control. Using real-time PCR, gene expressions of ALP, osteocalcin, and type 1 collagen were shown to be upregulated. As the method does not use any organic solvent, it eliminates problems associated with organic solvent residue and therefore improves the cell compatibility. It has a promising potential for the preparation of porous scaffold for bone tissue engineering.
authors.affiliation: State Key Laboratory of Oral diseases, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Zhao
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Li
authors.forename: Yun-qing
authors.initials: YQ
authors.lastname: Kang
authors.forename: Quan
authors.initials: Q
authors.lastname: Yuan
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D002130:Calcium Phosphates / Q000737:chemistry*; D048708:Cell Growth Processes / Q000502:physiology; D019245:Compressive Strength; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D011091:Polyesters; D011108:Polymers; C485817:beta-tricalcium phosphate; D019344:Lactic Acid; C033616:poly(lactide); C018392:tricalcium phosphate