title: One-step isolation of γ-oryzanol from rice bran oil by non-aqueous hydrostatic countercurrent chromatography.
abstract: The value-added γ-oryzanol was purified in one step from crude rice bran oil (RBO) using a preparative hydrostatic countercurrent chromatography (hydrostatic CCC) method, operating in the dual mode. The fractionation was performed using a non-aqueous biphasic solvent system consisting of heptane-acetonitrile-butanol (1.8:1.4:0.7, v/v/v), leading rapidly to the target compounds. Transfer of the analytical CCC method to large-scale isolation was also carried out yielding a high quantity-high purity fraction of γ-oryzanol. In addition, a fraction of hydroxylated triterpene alcohol ferulates (polar γ-oryzanol) was clearly separated and obtained. Furthermore, a fast HPLC-APCI(±)-HRMS method was developed and applied for the identification of γ-oryzanol as well as the polar γ-oryzanol in RBO and the resulting fractions. The purity of γ-oryzanol fraction was estimated as 97% based on HPLC-APCI-HRMS analysis.
authors.affiliation: Laboratory of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, University of Athens, Athens, Greece.
authors.forename: Apostolis
authors.initials: A
authors.lastname: Angelis
authors.forename: Aurélie
authors.initials: A
authors.lastname: Urbain
authors.forename: Maria
authors.initials: M
authors.lastname: Halabalaki
authors.forename: Nektarios
authors.initials: N
authors.lastname: Aligiannis
authors.forename: Alexios-Leandros
authors.initials: AL
authors.lastname: Skaltsounis
mesh_terms: D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D010666:Phenylpropionates / Q000302:isolation & purification*; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil; D013237:Stereoisomerism
chemical_list: D010666:Phenylpropionates; D010938:Plant Oils; D000073879:Rice Bran Oil; C013172:gamma-oryzanol