title: Cell cycle synchronization for the purpose of somatic cell nuclear transfer (SCNT).
abstract: Somatic cell nuclear transfer (SCNT) is a technically and biologically challenging procedure during which a differentiated committed nucleus undergoes rapid reprogramming into the totipotent state in a few hours. SCNT can be utilized to generate patient- and disease-specific embryonic stem cell (ESC) lines, which carry great promise in improving our understanding of major disease conditions and hope for better therapies. In this section, we will describe how mouse SCNT is performed and survey the importance of donor cell cycle synchronization and the methods to perform it.
authors.affiliation: Department of Obstetrics and Gynecology and the Hadassah Human Embryonic Stem Cell Research Center, Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, POB 12000, Jerusalem, 91120, Israel. yoelsh@ekmd.huji.ac.il
authors.forename: Yoel
authors.initials: Y
authors.lastname: Shufaro
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Reubinoff
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D003467:Cultural Deprivation; D016895:Culture Media, Serum-Free; D005260:Female; D006801:Humans; D051379:Mice; D050258:Mitosis Modulators / Q000494:pharmacology; D053652:Nuclear Transfer Techniques
chemical_list: D016895:Culture Media, Serum-Free; D050258:Mitosis Modulators