title: Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded tissue.
abstract: A combination of laser capture microdissection and reverse transcriptase real-time quantitative PCR -provides a powerful tool for the analysis of relative gene expression in archived tissue specimens. This chapter describes standard methodologies that can be used to determine the relative levels of gene expression in individual cells captured from formalin-fixed paraffin-embedded tissues.
authors.affiliation: Department of Microbiology and Immunology, Center for Molecular and Tumor Virology and Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center, Shreveport, LA, USA.
authors.forename: Ru
authors.initials: R
authors.lastname: Jiang
authors.forename: Rona S
authors.initials: RS
authors.lastname: Scott
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: Hutt-Fletcher
mesh_terms: D002469:Cell Separation / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008867:Microtomy / Q000379:methods; D021141:Nucleic Acid Amplification Techniques; D016612:Paraffin Embedding; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation
chemical_list: D012313:RNA