title: Detection of Mycobacterium avium ssp. paratuberculosis in ileocaecal lymph nodes collected from elderly slaughter cows using a semi-nested IS900 polymerase chain reaction.
abstract: The aim of this study was to investigate the occurrence of subclinical Mycobacterium avium spp. paratuberculosis (MAP) infections at slaughter by testing ileocaecal lymph nodes with a semi-nested IS900 PCR. Tissue samples were available within the framework of a parallel study investigating BSE-susceptibility factors in members of BSE-cohorts in the German Federal State of Lower Saxony. Ileocaecal lymph nodes were collected over a 2-year sampling period from 99 slaughter cattle of a mean age of 6.5 years (5.5-7.5 years). A recently developed IS900 semi-nested polymerase chain reaction (snPCR) assay offering a sensitivity of 1 genome equivalent was used for the detection of MAP-DNA. Based on this snPCR, 17 out of the 99 samples gave positive results, indicating a MAP occurrence of 17.17% in the random sample. All PCR products were sequenced for screening of polymorphisms. Nucleotide homologies of 98.5-100% were found with respect to the MAP K10 reference sequence IS900 (GenBank: AE16958). PCR analysis of ileocaecal lymph nodes collected from slaughter cattle proved to be a suitable technique to determine MAP occurrence in the local cattle population.
authors.affiliation: Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University, Burckhardtweg 2, D-37077 Göttingen, Germany.
authors.forename: Pia
authors.initials: P
authors.lastname: Münster
authors.forename: Inger
authors.initials: I
authors.lastname: Völkel
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Wemheuer
authors.forename: Jutta
authors.initials: J
authors.lastname: Petschenka
authors.forename: Wiebke
authors.initials: W
authors.lastname: Wemheuer
authors.forename: Christina
authors.initials: C
authors.lastname: Steinbrunn
authors.forename: Amely
authors.initials: A
authors.lastname: Campe
authors.forename: Walter J
authors.initials: WJ
authors.lastname: Schulz-Schaeffer
authors.forename: Lothar
authors.initials: L
authors.lastname: Kreienbrock
authors.forename: Claus-Peter
authors.initials: CP
authors.lastname: Czerny
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D005858:Germany / Q000453:epidemiology; D008198:Lymph Nodes / Q000382:microbiology*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D015995:Prevalence; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial