title: Artificial recombination may influence the evolutionary analysis of Newcastle disease virus.
abstract: The recombination rate in Newcastle disease virus (NDV) was as high as 10% in RDP analysis with full-length NDV genome sequences available in GenBank. We found that two NDV strains, China/Guangxi09/2003 and NDV/03/018, previously reported as recombinants, failed to show any evidence of recombination upon complete genome resequencing. Furthermore, we were able to reproduce artificial recombination by amplification of the M gene in a mixed sample of strains LaSota and ZJ1. It appears that the recombination of NDV is not as common as has been reported. NDV sequences in GenBank should be analyzed with caution during bioinformatic analyses for natural recombination events.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Song
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Cao
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Gu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.forename: Shunlin
authors.initials: S
authors.lastname: Hu
authors.forename: Hongquan
authors.initials: H
authors.lastname: Wan
authors.forename: Xiufan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D002681:China; D019143:Evolution, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral