title: Cytochrome P450 monooxygenases: an update on perspectives for synthetic application.
abstract: Cytochrome P450 monooxygenases (P450s) are versatile biocatalysts that catalyze the regio- and stereospeciﬁc oxidation of non-activated hydrocarbons under mild conditions, which is a challenging task for chemical catalysts. Over the past decade impressive advances have been achieved via protein engineering with regard to activity, stability and specificity of P450s. In addition, a large pool of newly annotated P450s has attracted much attention as a source for novel biocatalysts for oxidation. In this review we give a short up-to-date overview of recent results on P450 engineering for technical applications including aspects of whole-cell biocatalysis with engineered recombinant enzymes. Furthermore, we focus on recently identified P450s with novel biotechnologically relevant properties.
authors.affiliation: Institute of Biochemistry, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany. vlada.urlacher@uni-duesseldorf.de
authors.forename: Vlada B
authors.initials: VB
authors.lastname: Urlacher
authors.forename: Marco
authors.initials: M
authors.lastname: Girhard
mesh_terms: D000818:Animals; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics; D006801:Humans; D010084:Oxidation-Reduction; D015202:Protein Engineering; D055550:Protein Stability; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System