title: Elevated activity of dolichyl phosphate mannose synthase enhances biocontrol abilities of Trichoderma atroviride.
abstract: Antagonism of Trichoderma spp. against phytopathogenic fungi is widely exploited for biocontrol of plant diseases. A crucial role in the biocontrol mechanism is attributed to cell-wall-degrading enzymes secreted by Trichoderma spp. Therefore, more efficient production and secretion of the enzymes should elevate the biocontrol abilities of Trichoderma spp. Because the majority of secretory hydrolases are glycoproteins, it has been postulated that the posttranslational modification of these proteins could constitute a bottleneck in their production and secretion. Our previous study showed that improvement of O-glycosylation elevated protein secretion by Trichoderma reesei. In this study, we enhanced the biocontrol abilities of T. atroviride P1 against plant pathogens by overexpressing the Saccharomyces cerevisiae DPM1 gene coding for dolichyl phosphate mannose (DPM) synthase, a key enzyme in the O-glycosylation pathway. The transformants we obtained showed doubled DPM synthase activity and, at the same time, significantly elevated cellulolytic activity. They also revealed an improved antifungal activity against the plant pathogen Pythium ultimum.
authors.affiliation: Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Zembek
authors.forename: Urszula
authors.initials: U
authors.lastname: Perlinska-Lenart
authors.forename: Kurt
authors.initials: K
authors.lastname: Brunner
authors.forename: Barbara
authors.initials: B
authors.lastname: Reithner
authors.forename: Grazyna
authors.initials: G
authors.lastname: Palamarczyk
authors.forename: Robert L
authors.initials: RL
authors.lastname: Mach
authors.forename: Joanna S
authors.initials: JS
authors.lastname: Kruszewska
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D007887:Fabaceae / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D018525:Germination; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D010572:Pest Control, Biological; D010935:Plant Diseases / Q000382:microbiology*; D011775:Pythium / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D036226:Seedlings / Q000254:growth & development / Q000382:microbiology; D012639:Seeds / Q000382:microbiology / Q000502:physiology; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase