title: [Association of C602A and T1559C polymorphisms of E-selectin gene and essential hypertension].
abstract: OBJECTIVE
  To investigate the possible genetic associations between the C602A and T1559C polymorphisms of E-selectin (SELE) and essential hypertension.
  
  
  METHODS
  Essential hypertensive patients (n = 500) and healthy normotensive subjects (n = 930) were screened for the genotypes C602A and T1559C by real-time quantitative polymerase chain reaction after DNA extraction to identify representative variations in the SELE gene.
  
  
  RESULTS
  Normotensive subjects and hypertensive patients were significantly different with respect to the genotypes CC, CA and AA, 26 (5.2%), 20 (4.0%) and 454 (90.8%) vs 14 (1.5%), 53 (5.7%) and 863 (92.8%) respectively of C602A. And the C-allele frequency was also significantly different between the NT and EH groups (C, A = 7.2%, 92.8% vs 4.4%, 95.6%). When subgrouped by gender, frequency of CC, CA, AA between normotensive and essential hypertensive males was 14 (4.7%), 11 (3.7%), 272 (91.6%) and 10 (1.7%), 34 (5.8%), 545 (92.5%), which differed significantly (P < 0.05), while in female groups, all the frequency of genotypes were significantly different (P < 0.01) except CC + CA. The additive model (TT, TC, CC) of the T1559C genotype was significantly different between essential hypertensive and normotensive groups overall, 57 (11.4%), 200 (40.0%), 43 (48.6%) and 66 (7.1%), 354 (38.1%), 510 (54.8%), respectively. The T-allele of hypertensive patients significantly differed from normotensive subjects (T, C = 31.4%, 68.6% vs 26.1%, 73.9% respectively). When subgrouped by gender, between the male NT and EH groups, the TT, TC and CC frequency of T1559C were 36 (5.9%), 117 (39.4%), 144 (48.5%) and 35 (5.9%), 230 (39.0%), 354 (55.0%), and the frequency of T vs C was 31.4% vs 68.6% and 26.1% vs 73.9%, which were significantly different (all P < 0.01). As in female NT and EH groups, there were not significant differences existed at all.
  
  
  CONCLUSIONS
  C602A and T1559C of SELE are associated with essential hypertension in the Chinese population, and T1559C is closely related with male hypertension other than in females.
authors.affiliation: Department of Hypertension Beijing Institute of Heart, Lung & Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
authors.forename: Zuo-Guang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Qiu-Li
authors.initials: QL
authors.lastname: Niu
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.forename: Kuo
authors.initials: K
authors.lastname: Liu
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Lou
authors.forename: Ya
authors.initials: Y
authors.lastname: Liu
authors.forename: Jie-Lin
authors.initials: JL
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Wen
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Wen
authors.forename: Zhao-Su
authors.initials: ZS
authors.lastname: Wu
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D019040:E-Selectin / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D019040:E-Selectin; C546936:SELE protein, human