title: Dietary saturated fat/cholesterol, but not unsaturated fat or starch, induces C-reactive protein associated early atherosclerosis and ectopic fat deposition in diabetic pigs.
abstract: BACKGROUND
  Diabetes is thought to accelerate cardiovascular disease depending on the type of diet. This study in diabetic subjects was performed to investigate the metabolic, inflammatory and cardiovascular effects of nutritional components typically present in a Western, Mediterranean or high glycaemic diet.
  
  
  METHODS
  Streptozotocin-diabetic pigs (~45 kg) were fed for 10 weeks supplemental (40% of dietary energy) saturated fat/cholesterol (SFC), unsaturated fat (UF) or starch (S) in an eucaloric dietary intervention study.
  
  
  RESULTS
  Fasting plasma total, LDL and HDL cholesterol concentrations were 3-5 fold higher (p < 0.01) in SFC compared to UF and S pigs. Fasting plasma NEFA concentrations (mmol/L) were highest (p < 0.05) in SFC (1.09 ± 0.17), intermediate in UF (0.80 ± 0.14) and lowest in S pigs (0.58 ± 0.14) whereas plasma glucose (~13 mmol/L), triglyceride (~0.5 mmol/L) and insulin (~24 pmol/L) concentrations were comparable among SFC, UF and S pigs. The postprandial response area under the curves (AUC, 0-4 h) for glucose but not for insulin and triglyceride responses were intermediate in SFC (617 ± 144) and lowest (p < 0.05) in UF (378 ± 157) compared to S pigs (925 ± 139). Fasting hepatic glucose production, hepatic and peripheral insulin sensitivity and blood pressure were not different among pigs. C-reactive protein (CRP) concentrations (mg/L) were highest (p < 0.05) in SFC (25 ± 4), intermediate in S (21 ± 3) and lowest in UF pigs (14 ± 2). Liver weights, liver and muscle triglyceride concentrations, and the surface area of aorta fatty streaks were highest (p < 0.01) in SFC pigs. A positive correlation between postprandial plasma CRP and aorta fatty streaks was observed in SFC pigs (R(2) = 0.95). Retroperitoneal fat depot weight (g) was intermediate in SFC (260 ± 72), lowest in S (135 ± 51) and highest (p < 0.05) in UF (571 ± 95) pigs.
  
  
  CONCLUSIONS
  Dietary saturated fat/cholesterol induces inflammation, atherosclerosis and ectopic fat deposition whereas an equally high dietary unsaturated fat load does not induce these abnormalities and shows beneficial effects on postprandial glycaemia in diabetic pigs.
authors.affiliation: BioMedical Research of Wageningen University and Research Center, Lelystad, The Netherlands. sietsejan.koopmans@wur.nl
authors.forename: Sietse J
authors.initials: SJ
authors.lastname: Koopmans
authors.forename: Ruud
authors.initials: R
authors.lastname: Dekker
authors.forename: Mariette T
authors.initials: MT
authors.lastname: Ackermans
authors.forename: Hans P
authors.initials: HP
authors.lastname: Sauerwein
authors.forename: Mireille J
authors.initials: MJ
authors.lastname: Serlie
authors.forename: Heleen M M
authors.initials: HM
authors.lastname: van Beusekom
authors.forename: Mieke
authors.initials: M
authors.lastname: van den Heuvel
authors.forename: Wim J
authors.initials: WJ
authors.lastname: van der Giessen
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000209:etiology / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000502:physiology; D001823:Body Composition / Q000502:physiology; D050218:Body Fat Distribution; D002097:C-Reactive Protein / Q000378:metabolism*; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications / Q000378:metabolism*; D004041:Dietary Fats / Q000494:pharmacology*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004195:Disease Models, Animal; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007328:Insulin / Q000097:blood; D008297:Male; D019518:Postprandial Period; D013213:Starch / Q000494:pharmacology*; D013311:Streptozocin; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002791:Cholesterol, Dietary; D004041:Dietary Fats; D004042:Dietary Fats, Unsaturated; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D014280:Triglycerides; D013311:Streptozocin; D013213:Starch; D002097:C-Reactive Protein