title: Glycoprotein J of infectious laryngotracheitis virus is required for efficient egress of infectious virions from cells.
abstract: Glycoprotein J (gJ) of infectious laryngotracheitis virus (ILTV) represents a major viral antigen and is dispensable for replication in cell culture and chickens. We generated gJ deletion mutants derived from the United States Department of Agriculture standard challenge strain (USDA-ch), a GFP-expressing mutant GΔgJ, a gJ deletion mutant void of any foreign DNA insertion (BΔgJ) and a gJ rescue mutant gJR with US5 restored. GΔgJ, BΔgJ and gJR were characterized in cell culture and embryonated eggs. Entry kinetic assays showed that the gJ deletion mutants did not differ in their entry kinetics from gJR. Replication kinetics strongly indicated that gJ plays an important role during egress of the virus. Differences in the abilities of the mutants to replicate in chorioallantoic membranes of chicken embryos and to release infectious virus into the allantoic fluid supported a function of gJ during the egress of ILTV from infected cells.
authors.affiliation: Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Alice
authors.initials: A
authors.lastname: Mundt
authors.affiliation: Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Egbert
authors.initials: E
authors.lastname: Mundt
authors.affiliation: Anatomy and Radiology, Department of Population Health, University of Georgia, Athens, GA, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Hogan
authors.affiliation: Poultry Diagnostic and Research Center, University of Georgia, Athens, GA, USA.
authors.forename: Maricarmen
authors.initials: M
authors.lastname: García
mesh_terms: D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D017353:Gene Deletion; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D001352:Herpesvirus 1, Gallid / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D057074:Virus Release
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins