title: Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA).
abstract: OBJECTIVE
  To evaluate the performance of two antigenic preparations (vesicular fluid - VF and a glycoprotein fraction, LLa-Gp fraction, purified from a whole parasite extract by lentil lectin affinity chromatography) from Taenia solium cysticerci for the immunodiagnosis of neurocysticercosis.
  
  
  METHODS
  Fifty-six cerebrospinal fluid (CSF) samples (22 from patients with neurocysticercosis and 34 from patients with other neurological disorders) and 57 serum samples (22 from patients with neurocysticercosis, 18 from patients with other infections and 17 from presumably healthy persons) were assayed for anticysticercal IgG antibodies with an enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The VF ELISA showed 100% sensitivity and specificity in CSF and serum samples, whereas the sensitivity and specificity of the LLa-Gp ELISA were, respectively, 90.9% and 97.1%, with the CSF samples and 95.5% and 100% with serum samples. There was no significant difference in the sensitivity and specificity of the two antigenic preparations used to screen CSF and serum samples.
  
  
  CONCLUSIONS
  Considering the complexity and high cost of obtaining the LLa-Gp fraction, VF could be more suitable for screening specific antibodies by ELISA in CSF and serum samples from patients with neurocysticercosis.
authors.affiliation: Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, SP, Brazil.
authors.forename: Lisandra Akemi
authors.initials: LA
authors.lastname: Suzuki
authors.forename: Cláudio Lúcio
authors.initials: CL
authors.lastname: Rossi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000134:cerebrospinal fluid*; D000947:Antigens, Helminth / Q000276:immunology; D016022:Case-Control Studies; D002846:Chromatography, Affinity; D020420:Cyst Fluid / Q000276:immunology; D003552:Cysticercus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid*; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D037121:Plant Lectins / Q000276:immunology; D012680:Sensitivity and Specificity; D041201:Taenia solium / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006023:Glycoproteins; D007074:Immunoglobulin G; D037121:Plant Lectins; C033619:lentil lectin