title: Quantification of the Aspergillus versicolor allergen in house dust.
abstract: Aspergillus versicolor, a fungus commonly found on damp building materials, produces the allergen, Asp v 13. Here we report a sensitive Asp v 13 capture ELISA for A. versicolor spores and spore- and mycelial fragments in house dust samples. The method is based on a double polyclonal capture ELSIA. The detection limits for Asp v 13 antigen and A. versicolor spores without dust were 2.44 pg and 12 ng (ca. 110 spores). Detection limits for Asp v 13 and A. versicolor spores in sieved house dust samples were 1.0 ng and 7.8 μg per gram dry weight house dust, respectively. This detection limit is lower than for other house dust allergen immunoassays including for Stachybotrys chartarum, Aspergillus fumigatus, but much lower than that from Alternaria alternata.
authors.affiliation: Ottawa-Carleton Institute of Chemistry, Carleton University, Ottawa, ON K1S5B6, Canada.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Shi
authors.forename: Donald
authors.initials: D
authors.lastname: Belisle
authors.forename: J David
authors.initials: JD
authors.lastname: Miller
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D001230:Aspergillus / Q000276:immunology*; D004391:Dust / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057230:Limit of Detection; D013172:Spores, Fungal / Q000276:immunology
chemical_list: D000485:Allergens; D004391:Dust