title: SHOC1 and PTD form an XPF-ERCC1-like complex that is required for formation of class I crossovers.
abstract: Two distinct pathways for meiotic crossover formation coexist in most eukaryotes. The Arabidopsis SHOC1 protein is required for class I crossovers and shows sequence similarity with the XPF endonuclease family. Active XPF endonucleases form a heterodimer with ERCC1 proteins. Here, we show that PTD, an ERCC1-like protein, is required for class-I-interfering crossovers along with SHOC1, MSH4, MSH5, MER3 and MLH3. SHOC1 interacts with PTD in a two-hybrid assay, through its XPF-like nuclease-(HhH)(2) domain. We propose that a XPF-ERCC1-like heterodimer, represented by SHOC1 and PTD in Arabidopsis, involving Zip2 in Saccharomyces cerevisiae and C9orf84 in human, is required for formation of class I crossovers.
authors.affiliation: INRA, UMR 1318, Institut Jean-Pierre Bourgin, Route de Saint Cyr, 78026 Versailles, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Macaisne
authors.forename: Julien
authors.initials: J
authors.lastname: Vignard
authors.forename: Raphaël
authors.initials: R
authors.lastname: Mercier
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003434:Crossing Over, Genetic / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism*; D004843:Epistasis, Genetic / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D008540:Meiosis; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D011485:Protein Binding; D011506:Proteins; D012441:Saccharomyces cerevisiae
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D004268:DNA-Binding Proteins; C510978:PTD protein, Arabidopsis; D011506:Proteins; C498424:SLC39A2 protein, human; C561278:SPACA9 protein, human; D004706:Endodeoxyribonucleases; D004720:Endonucleases; C499359:Ercc1 protein, Arabidopsis; C568349:SHOC1 protein, Arabidopsis; C539269:XPF protein, Arabidopsis