title: Anti-inflammatory and antinociceptive activity of ouabain in mice.
abstract: Ouabain, an inhibitor of the Na(+)/K(+)-ATPase pump, was identified as an endogenous substance of human plasma. Ouabain has been studied for its ability to interfere with various regulatory mechanisms. Despite the studies portraying the ability of ouabain to modulate the immune response, little is known about the effect of this substance on the inflammatory process. The aim of this work was to study the effects triggered by ouabain on inflammation and nociceptive models. Ouabain produced a reduction in the mouse paw edema induced by carrageenan, compound 48/80 and zymosan. This anti-inflammatory potential might be related to the inhibition of prostaglandin E2, bradykinin, and mast-cell degranulation but not to histamine. Ouabain also modulated the inflammation induced by concanavalin A by inhibiting cell migration. Besides that, ouabain presented antinociceptive activity. Taken these data together, this work demonstrated, for the first time, that ouabain presented in vivo analgesic and anti-inflammatory effects.
authors.affiliation: Laboratório de Tecnologia Farmacêutica, Departamento de Fisiologia e Patologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa 58059-900, Brazil.
authors.forename: Danielle Ingrid Bezerra
authors.initials: DI
authors.lastname: de Vasconcelos
authors.forename: Jacqueline Alves
authors.initials: JA
authors.lastname: Leite
authors.forename: Luciana Teles
authors.initials: LT
authors.lastname: Carneiro
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Piuvezam
authors.forename: Maria Raquel Vitorino
authors.initials: MR
authors.lastname: de Lima
authors.forename: Liana Clébia Lima
authors.initials: LC
authors.lastname: de Morais
authors.forename: Vivian Mary
authors.initials: VM
authors.lastname: Rumjanek
authors.forename: Sandra
authors.initials: S
authors.lastname: Rodrigues-Mascarenhas
mesh_terms: D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects; D005260:Female; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D010042:Ouabain / Q000494:pharmacology / Q000627:therapeutic use*; D010147:Pain Measurement; D011957:Receptors, Opioid / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D011957:Receptors, Opioid; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase