title: Effect of diode-laser and AC magnetic field of bovine serum albumin nanospheres loaded with phthalocyanine and magnetic particles.
abstract: This study reports on the development and characterization of bovine serum albumin (BSA) nanospheres containing Silicon(IV) phthalocyanine (NzPc) and/or maghemite nanoparticles (MNP), the latter introduced via ionic magnetic fluid (MF). The nanosized BSA-loaded samples were designed for synergic application while combining Photodynamic Therapy and Hyperthermia. Incorporation of MNP in the albumin-based template, allowing full control of the magnetic content, was accomplished by adding a highly-stable ionic magnetic fluid sample to the albumin suspension, following heat denaturing. The material's evaluation was performed using Zeta potential measurements and scanning electron microscopy. The samples were characterized by steady-state techniques and time-resolved fluorescence. The in vitro assay, using human fibroblasts, revealed no cytotoxic effect in all samples investigated, demonstrating the potential of the tested system as a synergistic drug delivery system.
authors.affiliation: Departamento de Química, Grupo de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP 14040-901, Brazil.
authors.forename: Andreza Ribeiro
authors.initials: AR
authors.lastname: Simioni
authors.forename: Marcilene M A
authors.initials: MM
authors.lastname: Rodrigues
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Primo
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Morais
authors.forename: Antonio Claudio
authors.initials: AC
authors.lastname: Tedesco
mesh_terms: D002460:Cell Line; D004058:Diffusion; D004574:Electromagnetic Fields; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005347:Fibroblasts / Q000737:chemistry* / Q000528:radiation effects; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D054023:Lasers, Semiconductor; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000528:radiation effects; D053769:Nanocapsules / Q000737:chemistry* / Q000528:radiation effects; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects
chemical_list: D007211:Indoles; D054833:Isoindoles; D058185:Magnetite Nanoparticles; D053769:Nanocapsules; D012710:Serum Albumin, Bovine; C013647:phthalocyanine