title: New covalent capture probes for imaging and therapy, based on a combination of binding affinity and disulfide bond formation.
abstract: We describe the synthesis and development of new reactive DOTA-metal complexes for covalently targeting engineered receptors in vivo, which have superior tumor uptake and clearance properties for biomedical applications. These probes are found to clear efficiently through the kidneys and minimally through other routes, but bind persistently in the tumor target. We also explore the new technique of Cerenkov luminescence imaging to optically monitor radiolabeled probe distribution and kinetics in vivo. Cerenkov luminescence imaging uniquely enables sensitive noninvasive in vivo imaging of a β(-) emitter such as (90)Y with an optical imager.
authors.affiliation: Chemistry Department, University of California, Davis, California 95616, United States.
authors.forename: Tolulope A
authors.initials: TA
authors.lastname: Aweda
authors.forename: Vahid
authors.initials: V
authors.lastname: Eskandari
authors.forename: David L
authors.initials: DL
authors.lastname: Kukis
authors.forename: David L
authors.initials: DL
authors.lastname: Boucher
authors.forename: Bernadette V
authors.initials: BV
authors.lastname: Marquez
authors.forename: Heather E
authors.initials: HE
authors.lastname: Beck
authors.forename: Gregory S
authors.initials: GS
authors.lastname: Mitchell
authors.forename: Simon R
authors.initials: SR
authors.lastname: Cherry
authors.forename: Claude F
authors.initials: CF
authors.lastname: Meares
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods* / Q000451:nursing*; D004220:Disulfides; D049449:Luminescence; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000627:therapeutic use; D009369:Neoplasms / Q000175:diagnosis* / Q000188:drug therapy*; D011485:Protein Binding
chemical_list: D004220:Disulfides; D015335:Molecular Probes