title: [A preliminary study on the influence of human plasma exosomes-like vesicles on macrophage Wnt5A-Ca²+ pathway].
abstract: OBJECTIVE
  To study the influence of human plasma exosomes-like vesicles on the regulatory function of macrophages.
  
  
  METHODS
  The exosomes-like vesicles were purified from healthy donors plasma with a series of high-speed centrifugation and ultrafiltration. Macrophages were derived from cultured human blood monocytes. The molecular markers of macrophages were assayed by FACS. After cultured with exosomes-like vesicles, the changes of macrophages cytoplasma Ca(2+), and related genes and proteins were assayed by FACS, RT-PCR and Western Blot, respectively.
  
  
  RESULTS
  After cultured with exosomes-like vesicles, mean fluorescent intensity (MFI) of macrophages cytoplasma Ca(2+) was increased. The vesicles enhanced macrophages to express cytokines genes, the expression of IL-1β and TNF-α genes being increased by 0.85 and 1.69 times respectively at 2 h, and that of IL-6 gene 3.7 times compared with the control at 8 h. However, the vesicles inhibited the expression of macrophages IL-10 gene, had no influence on the Frizzled5 receptor expression and could induce CaMKII phosphorylation.
  
  
  CONCLUSIONS
  Exosomes-like vesicles can up-regulat macrophages expression of inflammatory cytokines genes, and increase the secretion of inflammatory cytokines by activating the Wnt5A-Ca(2+) signaling pathway.
authors.affiliation: Huashan Hospital of Fudan University united by Blood Center, Shanghai 200040, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Ya-Na
authors.initials: YN
authors.lastname: Ren
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Yang
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Ru-Feng
authors.initials: RF
authors.lastname: Xie
authors.forename: Hua-Hua
authors.initials: HH
authors.lastname: Fan
mesh_terms: D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D055354:Exosomes; D005260:Female; D006801:Humans; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051153:Wnt Proteins / Q000378:metabolism*; D000071818:Wnt-5a Protein; D055815:Young Adult
chemical_list: D011518:Proto-Oncogene Proteins; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D002118:Calcium