title: An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification.
abstract: A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, Fujian, 350002, China. zylin@fzu.edu.cn
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Yang
authors.forename: Guiyun
authors.initials: G
authors.lastname: Zhang
authors.forename: Qida
authors.initials: Q
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000235:genetics; D021881:DNA, Catalytic / Q000378:metabolism; D004720:Endonucleases / Q000378:metabolism; D016158:Genes, p53; D005821:Genetic Techniques; D006801:Humans; D057230:Limit of Detection; D009154:Mutation; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D021881:DNA, Catalytic; D016159:Tumor Suppressor Protein p53; D004247:DNA; D004720:Endonucleases