title: Mesenchymal stem cell therapy in necrotizing enterocolitis: a rat study.
abstract: We evaluated the potential therapeutic use of exogenous human bone marrow-derived mesenchymal stem cells (hBM-MSCs) in an experimental rat model of necrotizing enterocolitis (NEC). Thirty-six newborn Sprague-Dawley rats were randomly divided into three groups: NEC, NEC + hBM-MSC, and a control (control and control + hBM-MSC). NEC was induced by enteral formula feeding, exposure to hypoxia-hyperoxia, and cold stress. After NEC was induced, iron-labeled hBM-MSCs were administered by intraperitoneal injection. All pups were killed on the fourth day following injection, and the terminal ileum was excised for a histopathological and immunohistochemical evaluation. The pups in the NEC + hBM-MSC group showed significant weight gains and improvements in their clinical sickness scores (p < 0.01). Bowel damage severity observed in the histopathological evaluation was significantly lower in the NEC + hBM-MSC group than that in the NEC group (p = 0.012). The number of MSCs homing to the bowel was significantly higher in the NEC + hBM-MSC group than that in the control + hBM-MSC group. In conclusion, this is the first study that has evaluated the effectiveness of hBM-MSCs in a neonatal rat NEC model. MSCs reduced histopathological damage significantly.
authors.affiliation: Department of Neonatology, Fatih University Faculty of Medicine, Ankara 06510, Turkey. ctayman22@gmail.com
authors.forename: Cüneyt
authors.initials: C
authors.lastname: Tayman
authors.forename: Duygu
authors.initials: D
authors.lastname: Uckan
authors.forename: Emine
authors.initials: E
authors.lastname: Kilic
authors.forename: Ahmet Tulga
authors.initials: AT
authors.lastname: Ulus
authors.forename: Alparslan
authors.initials: A
authors.lastname: Tonbul
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Murat Hirfanoglu
authors.forename: Fatma
authors.initials: F
authors.lastname: Helvacioglu
authors.forename: Hacer
authors.initials: H
authors.lastname: Haltas
authors.forename: Burhan
authors.initials: B
authors.lastname: Koseoglu
authors.forename: M Mansur
authors.initials: MM
authors.lastname: Tatli
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D020345:Enterocolitis, Necrotizing / Q000473:pathology / Q000628:therapy*; D005290:Ferric Compounds; D006652:Histological Techniques; D006801:Humans; D007082:Ileum / Q000473:pathology; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007274:Injections, Intraperitoneal; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D014183:Transplantation, Heterologous
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide