title: Matrine attenuates endotoxin-induced acute liver injury after hepatic ischemia/reperfusion in rats.
abstract: OBJECTIVE
  Hepatic ischemia/reperfusion (HIR) injury is an unavoidable consequence of major liver surgery, during which endotoxemia often takes place. This study aimed to investigate whether matrine has a protective effect against HIR-induced liver injury aggravated by endotoxin.
  
  
  METHODS
  Wistar rats were subjected to 30 min of HIR followed by lipopolysaccharide (LPS) (0.5 mg/kg) administration. At the indicated time points, six rats from each group (24 rats) were randomly euthanized to collect blood samples and livers.
  
  
  RESULTS
  Preadministration of matrine protected livers from injury induced by HIR+LPS as the histological score, myeloperoxidase activity and malondialdehyde contents, expression of macrophage-inflammatory protein-2, DNA-binding activity of nuclear factor κB, and serum levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, tumor necrosis factor-α, soluble intercellular adhesion molecule-1, and nitric oxide were significantly reduced, and serum levels of interleukin-6 were further increased. HIR+LPS markedly induced cell apoptosis and necrosis, and upregulated the expression of cleaved caspase-3, Fas, and FasL. Matrine significantly reduced cell necrosis, but had a nonsignificant inhibitory effect on cell apoptosis and expression of cleaved caspase-3 and FasL.
  
  
  CONCLUSIONS
  Matrine attenuates endotoxin-induced acute liver injury after HIR mainly by its anti-inflammatory and antioxidative activities, and has little inhibitory effect on cell apoptosis.
authors.affiliation: Department of General Surgery, Fifth Affiliated Hospital of Harbin Medical University, Daqing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.forename: Liquan
authors.initials: L
authors.lastname: Tong
authors.forename: Haiquan
authors.initials: H
authors.lastname: Qiao
authors.forename: Xinlei
authors.initials: X
authors.lastname: Li
authors.forename: Liguang
authors.initials: L
authors.lastname: You
authors.forename: Hongchi
authors.initials: H
authors.lastname: Jiang
authors.forename: Xueying
authors.initials: X
authors.lastname: Sun
mesh_terms: D000470:Alkaloids / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D019446:Endotoxemia / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008070:Lipopolysaccharides; D008107:Liver Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D011807:Quinolizines / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D008070:Lipopolysaccharides; D011807:Quinolizines; D000093842:Matrines