title: Dnmt1/Transcription factor interactions: an alternative mechanism of DNA methylation inheritance.
abstract: DNA methylation inheritance is the process of copying, via the DNA methyltransferase 1 (Dnmt1), the pre-existing methylation patterns onto the new DNA strand during DNA replication. Experiments of chromatin immunoprecipitation, measurement of maintenance methyltransferase activity, proximity ligation in situ assays (P-LISA, Duolink/Olink), and transcription factor arrays demonstrate that Dnmt1 interacts with transcription factors to promote site-specific DNA methylation inheritance, while the Dnmt1-PCNA-UHRF1 complex promotes the DNA methylation inheritance without site preference. We also show that the Dnmt1-PCNA-UHRF1 and Dnmt1/transcription factor complexes methylate DNA by acting as a single player or in cooperation. Thus, our data establish that the copying of the pre-existing methylation pattern is governed by the orchestration of the untargeted and the targeted mechanisms of DNA methylation inheritance, which are themselves dictated by the partners of Dnmt1.
authors.affiliation: Institut de Recherche Thérapeutique de l'Université de Nantes, INSERM U892, Centre de Recherche en Cancérologie Nantes-Angers, Equipe Aspect Mécanistiques et Physiopathologiques de l'Activité des Protéines de la Famille de Bcl-2, Equipe Labellisée Ligue Nationale Contre le Cancer, Nantes, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Hervouet
authors.forename: François M
authors.initials: FM
authors.lastname: Vallette
authors.forename: Pierre-François
authors.initials: PF
authors.lastname: Cartron