title: A method to extract intact and pure RNA from mycobacteria.
abstract: We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.
authors.affiliation: Combi Chem Bio Resource Center, Organic Chemical Division, National Chemical Laboratory, Pune, Maharashtra 411008, India.
authors.forename: Shamim
authors.initials: S
authors.lastname: Akhtar
authors.forename: Sampa
authors.initials: S
authors.lastname: Sarkar
authors.forename: Abhishek
authors.initials: A
authors.lastname: Mishra
authors.forename: Dhiman
authors.initials: D
authors.lastname: Sarkar
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D000860:Hypoxia; D009161:Mycobacterium / Q000737:chemistry*; D009163:Mycobacterium bovis / Q000737:chemistry; D009168:Mycobacterium phlei / Q000737:chemistry; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D012313:RNA / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013104:Spheroplasts / Q000737:chemistry
chemical_list: D012329:RNA, Bacterial; D012313:RNA