title: Proteomics in the liver of gilthead sea bream (Sparus aurata) to elucidate the cellular response induced by the intake of maslinic acid.
abstract: Maslinic acid (MA) is a pentacyclic triterpene used as a feed additive to stimulate growth, protein-turnover rates, and hyperplasia in fish. To further our understanding of cellular mechanisms underlying the action of MA, we have used 2-DE coupled with MS to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (Sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg MA-enriched diet (MA(100)). After the comparison of the protein profiles from MA(100) fed fish and from control, 49 protein spots were found to be altered in abundance (≥2-fold). Analysis by MALDI-TOF/TOF allowed the unambiguous identification of 29 spots, corresponding to 19 different proteins. These proteins were: phosphoglucomutase, phosphoglucose isomerase, S-adenosyl methionine-dependent methyltransferase class I, aldehyde dehydrogenase, catalase, 6-phosphogluconate dehydrogenase, fumarylacetoacetate hydrolase, 4-hydroxyphenylpyruvic dioxygenase, methylmalonate-semialdehyde dehydrogenase, lysozyme, urate oxidase, elongation factor 2, 60 kDa heat-shock protein, 58 kDa glucose-regulated protein, cytokeratin E7, type-II keratin, intermediate filament proteins, 17-β-hydroxysteroid dehydrogenase type 4, and kinase suppressor of Ras1. Western blot analysis of kinase suppressor of Ras1, glucose 6-phosphate dehydrogenase, elongation factor 2, 60 kDa heat-shock protein, and catalase supported the proteome evidence. Based on the changes found in the protein-expression levels of these proteins, we proposed a cellular-signalling pathway to explain the hepatic-cell response to the intake of a diet containing MA.
authors.affiliation: Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Rufino-Palomares
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Reyes-Zurita
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Fuentes-Almagro
authors.forename: Manuel
authors.initials: M
authors.lastname: de la Higuera
authors.forename: José A
authors.initials: JA
authors.lastname: Lupiáñez
authors.forename: Juan
authors.initials: J
authors.lastname: Peragón
mesh_terms: D000704:Analysis of Variance; D000821:Animal Feed; D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D029941:Fish Proteins / Q000032:analysis* / Q000378:metabolism; D005398:Fisheries; D005947:Glucose / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D015596:Nutrition Assessment; D010446:Peptide Fragments / Q000378:metabolism; D010449:Peptide Mapping; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D021541:Sea Bream / Q000254:growth & development / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014315:Triterpenes / Q000494:pharmacology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D029941:Fish Proteins; D005947:Glucose; D010446:Peptide Fragments; D020543:Proteome; D014315:Triterpenes; C412811:maslinic acid; D009828:Oleanolic Acid