title: Suppression of dust mite extract and 2,4-dinitrochlorobenzene-induced atopic dermatitis by the water extract of Lindera obtusiloba.
abstract: BACKGROUND
  The Lindera obtusiloba has been used in traditional medicine for the treatment of inflammation and dermatitis. In this study, we investigated the effect of topical application of Lindera obtusiloba water extract (LOWE) on the house dust mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD).
  
  
  METHODS
  We established AD model in BALB/c mice by repeated local exposure of DFE/DNCB to the ears. After a topical application of LOWE on the skin lesions, the epidermal thickness, mast cell infiltration, and serum immunoglobulin E (IgE) and histamine were measured. In addition, the gene expression of interleukin (IL)-4, IL-13, IL-31, and tumor necrosis factor (TNF)-α in the ears was assayed.
  
  
  RESULTS
  LOWE reduced AD symptoms based on ear thickness, histopathological analysis, and serum IgE levels. LOWE inhibited mast cell infiltration into the ear and elevation of serum histamine in AD model. Moreover, LOWE suppressed DFE/DNCB-induced expression of IL-4, IL-13, IL-31, and TNF-α in the ears.
  
  
  CONCLUSIONS
  Our results showed that topical application of LOWE exerts beneficial effects in AD symptoms, suggesting that LOWE might be a candidate for the treatment of AD.
authors.affiliation: Division of Sport Science, College of Natural Sciences, Konkuk University, Chungbuk 380-702, Republic of Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Choi
authors.forename: Soyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Hui-Hun
authors.initials: HH
authors.lastname: Kim
authors.forename: Thoudam S K
authors.initials: TS
authors.lastname: Singh
authors.forename: Jin Kyeong
authors.initials: JK
authors.lastname: Choi
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Choi
authors.forename: Won Mo
authors.initials: WM
authors.lastname: Suh
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D039741:Antigens, Dermatophagoides; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D004137:Dinitrochlorobenzene; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004423:Ear; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D018793:Interleukin-13 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D007378:Interleukins / Q000235:genetics; D027443:Lindera / Q000737:chemistry; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D012997:Solvents / Q000737:chemistry*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics; D014867:Water / Q000737:chemistry*
chemical_list: D018926:Anti-Allergic Agents; D039741:Antigens, Dermatophagoides; D004137:Dinitrochlorobenzene; D018793:Interleukin-13; D007378:Interleukins; D010936:Plant Extracts; D012997:Solvents; D014409:Tumor Necrosis Factor-alpha; C502588:interleukin-31, mouse; D014867:Water; D015847:Interleukin-4; D007073:Immunoglobulin E; D006632:Histamine