title: Enhancement of methane production from cassava residues by biological pretreatment using a constructed microbial consortium.
abstract: In the study, a stable thermophilic microbial consortium with high cellulose-degradation ability was successfully constructed. That several species of microbes coexisted in this consortium was proved by DGGE (denaturing gradient gel electrophoresis) and sequence analysis. The cooperation and symbiosis of these microbes in this consortium enhanced their cellulose-degradation ability. The pretreatment of cassava residues mixing with distillery wastewater prior to anaerobic digestion was investigated by using this microbial consortium as inoculums in batch bioreactors at 55 °C. The experimental results showed that the maximum methane yield (259.46 mL/g-VS) of cassava residues was obtained through 12h of pretreatment by this microbial consortium, which was 96.63% higher than the control (131.95 mL/g-VS). In addition, it was also found that the maximum methane yield is obtained when the highest filter paper cellulase (FPase), carboxymethyl cellulase (CMCase) and xylanase activity and soluble COD (sCOD) are produced.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Tian
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Mao
authors.forename: Lei
authors.initials: L
authors.lastname: Tang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Hongjian
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001421:Bacteria, Anaerobic / Q000235:genetics / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002482:Cellulose / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D058645:Denaturing Gradient Gel Electrophoresis; D002365:Manihot / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D059013:Microbial Consortia / Q000235:genetics*; D008855:Microscopy, Electron, Scanning; D012037:Refuse Disposal / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D002482:Cellulose; D008697:Methane