title: Identification and characterization of five new classes of chlorogenic acids in burdock (Arctium lappa L.) roots by liquid chromatography/tandem mass spectrometry.
abstract: Burdock (Arcticum lappa L.) roots are used in folk medicine and also as a vegetable in Asian countries especially Japan, Korea, and Thailand. We have used LC-MS(n) (n = 2-4) to detect and characterize in burdock roots 15 quantitatively minor fumaric, succinic, and malic acid-containing chlorogenic acids, 11 of them not previously reported in nature. These comprise 3-succinoyl-4,5-dicaffeoyl or 1-succinoyl-3,4-dicaffeoylquinic acid, 1,5-dicaffeoyl-3-succinoylquinic acid, 1,5-dicaffeoyl-4-succinoylquinic acid, and 3,4-dicaffeoyl-5-succinoylquinic acid (M(r) 616); 1,3-dicaffeoyl-5-fumaroylquinic acid and 1,5-dicaffeoyl-4-fumaroylquinic acid (M(r) 614); 1,5-dicaffeoyl-3-maloylquinic acid, 1,4-dicaffeoyl-3-maloylquinic acid, and 1,5-dicaffeoyl-4-maloylquinic acid (M(r) 632); 1,3,5-tricaffeoyl-4-succinoylquinic acid (M(r) 778); 1,5-dicaffeoyl-3,4-disuccinoylquinic acid (M(r) 716); 1,5-dicaffeoyl-3-fumaroyl-4-succinoylquinic acid and 1-fumaroyl-3,5-dicaffeoyl-4-succinoylquinic acid (M(r) 714); dicaffeoyl-dimaloylquinic acid (M(r) 748); and 1,5-dicaffeoyl-3-succinoyl-4-dimaloylquinic acid (M(r) 732). All the structures have been assigned on the basis of LC-MS(n) patterns of fragmentation, relative hydrophobicity, and analogy of fragmentation patterns if compared to caffeoylquinic acids.
authors.affiliation: Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Jaiswal
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kuhnert
mesh_terms: D031170:Arctium / Q000737:chemistry*; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000145:classification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008519:Medicine, Traditional; D018517:Plant Roots / Q000737:chemistry*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002726:Chlorogenic Acid; C478100:3,4-di-O-caffeoylquinic acid