title: Magnetic/polymer/nanogold complex using as a novel enzyme support.
abstract: A novel enzyme support based on magnetic/polymer/nanogold complex was prepared in this study. At first, carboxymethyl chitosan (CMC) were connected onto the surface of Fe3O4 nanoparticles, and then gold nanoparticles were in situ synthesized and bound to CMC chains, resulting in Fe3O4/CMC/nanogold complex. The complex was determined by UV-vis spectroscopy, transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Horseradish peroxidase (HRP) was immobilized on the Fe3O4/CMC/nanogold complex. Compared with free HRP, the immobilized HRP exhibits higher catalytic activity and better storage stability. This Fe3O4/CMC/nanogold complex can serve as an excellent support for enzymes.
authors.affiliation: Hunan Key Laboratory of Green-Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008, PR China.
authors.forename: Libo
authors.initials: L
authors.lastname: Nie
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Zejun
authors.initials: Z
authors.lastname: Wu
mesh_terms: D019149:Bioreactors; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D002942:Circular Dichroism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; C514968:carboxymethyl-chitosan; D006046:Gold; D048271:Chitosan; D006735:Horseradish Peroxidase