title: Establishment of polycystic ovary syndrome-derived human embryonic stem cell lines.
abstract: BACKGROUND
  Recently, human embryonic stem cells (hESCs) of some genetic diseases have been established, but little research has been done on polycystic ovary syndrome (PCOS)-derived hESCs. The establishment of PCOS-derived hESCs provides a biological basis for exploring the pathogenesis, gene mapping and gene therapy of PCOS.
  
  
  METHODS
  Discarded fresh embryos were collected and cultured until the blastocyst stage, and then inner cell masses (ICM) were isolated by mechanical methods and incubated in the mixed feeder layer containing human stem cell medium. hESCs were identified whether to maintain normal karyotype and pluripotency by alkaline phosphates (AKP), stage-specific embryonic antigen-4 (SSEA-4), NANOG, SOX2 and TRA-1-60, octamer binding protein 4(OCT-4), and in vivo and in vitro differentiation.
  
  
  RESULTS
  Of the 11 passaged ICM, nine showed adherent growths within 48 h with an adherence rate of 81.8% (9/11). Five PCOS-derived hESCs were established and all of them have the characteristics of pluripotent differentiation. One was from 2PN embryo which was retarded in the cleavage stage, one was from 1PN embryo and others were from 0PN embryo. They were named p-hES-1, p-hES-2, p-hES-3, p-hES-4, p-hES-5, respectively.
  
  
  CONCLUSIONS
  We provide biological models for studying the pathogenesiss of PCOS.
authors.affiliation: Reproductive Medical Center, Department of Obstetrics and Gynecology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Peng-fen
authors.initials: PF
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Kong
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.forename: Ai-hong
authors.initials: AH
authors.lastname: Bai
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Ying-pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002469:Cell Separation; D019924:Embryo Disposition; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells / Q000378:metabolism / Q000473:pathology*; D061252:Feeder Cells / Q000166:cytology; D005260:Female; D005307:Fertilization in Vitro; D052816:Foreskin / Q000166:cytology; D006801:Humans; D008297:Male; D011085:Polycystic Ovary Syndrome / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D061251:Primary Cell Culture / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers