title: Influence of low molecular (below 5 KD) fraction from cord blood and actovegin on phagocytic activity of frozen-thawed neutrophils.
abstract: The influence of the cattle cord blood low-molecular fraction (CBF below 5 kDa) as part of the rehabilitating medium in comparison with Actovegin on the functional activity of neutrophils after cryopreservation was studied. Incubation of frozen-thawed neutrophils in the rehabilitating media containing the low-molecular fraction or Actovegin stimulates their phagocytic function, in particular engulfing and digesting ability. After incubation of frozen-thawed neutrophils in the media containing 0.15 mg per ml CBF or 1.5 mg per ml Actovegin, their oxygen-dependent metabolism was activated, since the number of NBT-positive neutrophils increased significantly in comparison with the control. Gel-penetrating chromatography of CBF and Actovegin revealed differences between their chromatograms reflecting differences between the compositions compared. The recovery of the functional activity of frozen-thawed neutrophils was possible in the media containing the cattle cord blood low-molecular fraction (below 5 kDa) or Actovegin at 0.15 mg per ml and 1.5 mg per ml, respectively.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine.
authors.forename: A K
authors.initials: AK
authors.lastname: Gulevsky
authors.forename: N N
authors.initials: NN
authors.lastname: Moiseyeva
authors.forename: O L
authors.initials: OL
authors.lastname: Gorina
mesh_terms: D000818:Animals; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods*; D002470:Cell Survival; D002850:Chromatography, Gel; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005312:Fetal Blood / Q000737:chemistry*; D005333:Fetus; D006418:Heme / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D018375:Neutrophil Activation; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D013211:Staphylococcus aureus
chemical_list: D003451:Cryoprotective Agents; D006418:Heme; C015646:Actovegin