title: Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
abstract: We evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying aeromonads with an extraction procedure. Genus-level accuracy was 100%. Compared to rpoB gene sequencing, species-level accuracy was 90.6% (29/32) for type and reference strains and 91.4% for a collection of 139 clinical and environmental isolates, making this system one of the most accurate and rapid methods for phenotypic identification. The reliability of this technique was very promising, although some improvements in database composition, taxonomy, and discriminatory power are needed.
authors.affiliation: Centre Hospitalier Universitaire de Montpellier, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, Montpellier, France. brigitte_lamy@yahoo.fr
authors.forename: Brigitte
authors.initials: B
authors.lastname: Lamy
authors.forename: Angeli
authors.initials: A
authors.lastname: Kodjo
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000191:economics; D016208:Databases, Factual; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics