title: Mobilization of bone marrow cells by CSF3 protects mice from bleomycin-induced lung injury.
abstract: BACKGROUND
  Bone marrow-derived cells may play a role in tissue injury and repair. Growth factors facilitate the mobilization of bone marrow-derived cells to the site of injury.
  
  
  OBJECTIVE
  The aim of this study was to determine the effect of the mobilization of autologous bone marrow-derived cells by granulocyte colony-stimulating factor (CSF3) on bleomycin-induced lung injury in mice.
  
  
  METHODS
  The bone marrow from male green fluorescent protein transgenic (C57Bl/6J) mice was transplanted into irradiated female C57Bl/6J mice. Bleomycin lung injury was induced in these bone marrow-reconstituted mice and unreconstituted C57Bl/6J mice, and some mice were treated with recombinant CSF3. Lung histology, survival, cytokine expression and matrix metalloproteinase (MMP) expression were evaluated to determine the effect of CSF3 after bleomycin-induced lung injury.
  
  
  RESULTS
  Histology and flow cytometry analysis showed successful mobilization of bone marrow-derived cells by CSF3 treatment in the recipient lungs. Importantly, CSF3 attenuated bleomycin-induced lung injury and improved survival. Furthermore, CSF3 administration regulated transforming growth factor-β, interferon-γ, MMP9 and tissue inhibitors of MMP1 expression during bleomycin injury.
  
  
  CONCLUSIONS
  These data demonstrated that the mobilization of bone marrow-derived cells by CSF3 has a protective effect against bleomycin-induced lung injury and fibrosis.
authors.affiliation: The First Hospital, Suzhou University, Suzhou, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Han-Shui
authors.initials: HS
authors.lastname: Jiang
authors.forename: Xue-Yuan
authors.initials: XY
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Li
authors.forename: Rong-Xuan
authors.initials: RX
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Zheng
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Chu
authors.forename: Xue-Jin
authors.initials: XJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000187:drug effects / Q000378:metabolism*; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000473:pathology* / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000903:Antibiotics, Antineoplastic; D011994:Recombinant Proteins; D001761:Bleomycin; D016179:Granulocyte Colony-Stimulating Factor; D049452:Green Fluorescent Proteins; D020782:Matrix Metalloproteinases