title: Purification of tubulin from porcine brain.
abstract: Microtubules, polymers of the heterodimeric protein αβ-tubulin, give shape to cells and are the tracks for vesicle transport and chromosome segregation. In vitro assays to study microtubule functions and their regulation by microtubule-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. gell@mpi-cbg.de
authors.forename: Christopher
authors.initials: C
authors.lastname: Gell
authors.forename: Claire T
authors.initials: CT
authors.lastname: Friel
authors.forename: Barbara
authors.initials: B
authors.lastname: Borgonovo
authors.forename: David N
authors.initials: DN
authors.lastname: Drechsel
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.forename: Jonathon
authors.initials: J
authors.lastname: Howard
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D013552:Swine; D014404:Tubulin / Q000302:isolation & purification*
chemical_list: D014404:Tubulin