title: Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse.
abstract: OBJECTIVE
  To analysis and identify a bacterium strain isolated from laboratory breeding mouse far away from a hospital.
  
  
  METHODS
  Phenotype of the isolate was investigated by conventional microbiological methods, including Gram-staining, colony morphology, tests for haemolysis, catalase, coagulase, and antimicrobial susceptibility test. The mecA and 16S rRNA genes were amplified by the polymerase chain reaction (PCR) and sequenced. The base sequence of the PCR product was compared with known 16S rRNA gene sequences in the GenBank database by phylogenetic analysis and multiple sequence alignment.
  
  
  RESULTS
  The isolate in this study was a gram positive, coagulase negative, and catalase positive coccus. The isolate was resistant to oxacillin, methicillin, penicillin, ampicillin, cefazolin, ciprofloxacin erythromycin, et al. PCR results indicated that the isolate was mecA gene positive and its 16S rRNA was 1 465 bp. Phylogenetic analysis of the resultant 16S rRNA indicated the isolate belonged to genus Saphylococcus, and multiple sequence alignment showed that the isolate was Saphylococcus haemolyticus with only one base difference from the corresponding 16S rRNA deposited in the GenBank.
  
  
  CONCLUSIONS
  16S rRNA gene sequencing is a suitable technique for non-specialist researchers. Laboratory animals are possible sources of lethal pathogens, and researchers must adapt protective measures when they manipulate animals.
authors.affiliation: Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China.
authors.forename: Fengying
authors.initials: F
authors.lastname: Huang
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Meng
authors.forename: Guanghong
authors.initials: G
authors.lastname: Tan
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Wenli
authors.initials: W
authors.lastname: Mei
authors.forename: Haofu
authors.initials: H
authors.lastname: Dai
mesh_terms: D000818:Animals; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D051379:Mice / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041162:Staphylococcus haemolyticus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*
chemical_list: D012336:RNA, Ribosomal, 16S