title: Identification of NF-κB modulation capabilities within human intestinal commensal bacteria.
abstract: The intestinal microbiota plays an important role in modulation of mucosal immune responses. To seek interactions between intestinal epithelial cells (IEC) and commensal bacteria, we screened 49 commensal strains for their capacity to modulate NF-κB. We used HT-29/kb-seap-25 and Caco-2/kb-seap-7 intestinal epithelial cells and monocyte-like THP-1 blue reporter cells to measure effects of commensal bacteria on cellular expression of a reporter system for NF-κB. Bacteria conditioned media (CM) were tested alone or together with an activator of NF-κB to explore its inhibitory potentials. CM from 8 or 10 different commensal species activated NF-κB expression on HT-29 and Caco-2 cells, respectively. On THP-1, CM from all but 5 commensal strains stimulated NF-κB. Upon challenge with TNF-α or IL-1β, some CM prevented induced NF-κB activation, whereas others enhanced it. Interestingly, the enhancing effect of some CM was correlated with the presence of butyrate and propionate. Characterization of the effects of the identified bacteria and their implications in human health awaits further investigations.
authors.affiliation: INRA, UMR1319, 78350 Jouy-en-Josas, France.
authors.forename: Omar
authors.initials: O
authors.lastname: Lakhdari
authors.forename: Julien
authors.initials: J
authors.lastname: Tap
authors.forename: Fabienne
authors.initials: F
authors.lastname: Béguet-Crespel
authors.forename: Karine
authors.initials: K
authors.lastname: Le Roux
authors.forename: Tomas
authors.initials: T
authors.lastname: de Wouters
authors.forename: Antonietta
authors.initials: A
authors.lastname: Cultrone
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Nepelska
authors.forename: Fabrice
authors.initials: F
authors.lastname: Lefèvre
authors.forename: Joël
authors.initials: J
authors.lastname: Doré
authors.forename: Hervé M
authors.initials: HM
authors.lastname: Blottière
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000378:metabolism*; D018938:Caco-2 Cells; D018929:Cell Culture Techniques; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D015870:Gene Expression / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000737:chemistry / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology* / Q000382:microbiology*; D009000:Monocytes; D016328:NF-kappa B / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D053583:Interleukin-1beta; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha