title: Newly identified respiratory viruses associated with acute lower respiratory tract infections in children in Lanzou, China, from 2006 to 2009.
abstract: Nasopharyngeal aspirates were collected from 813 children ≤ 14 years old with acute lower respiratory tract infections in Lanzhou, China, from December 2006 to November 2009. PCR or RT-PCR was used to screen for the presence of 10 respiratory viruses. Viral agents were identified in 73.92% (601/813) of specimens, including RSV in 40.71%, hMPV in 6.15%, IFVA in 7.13%, IFVB in 0.98%, PIV1-3 in 7.87%, HCoV-HKU1 in 2.21%, HCoV-NL63 in 3.81%, HRV in 19.93%, AdV in 7.50% and HBoV in 11.56%. Two or more viruses were detected in 34.44% (280/813) of cases. The newly identified respiratory viruses, HBoV, hMPV, HCoV-HKU1 and HCoV-NL63, accounted for 22.01% of the detected viral pathogens. RSV and HRV were frequently detected in patients with bronchiolitis, and hMPV was frequently associated with pneumonia. HCoV-NL63 was found to be one of the causative agents of acute respiratory wheezing in young children. No seasonal variation was found in the incidence of detection of HCoV-HKU1, HCoV-NL63 or HBoV. This 3-year study demonstrated that viral pathogens play an important role in children with ALRTIs, and more attention should be paid to these newly identified viral agents.
authors.affiliation: Medical School of Nanjing University, Nanjing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: R-F
authors.initials: RF
authors.lastname: Zhang
authors.forename: Z-P
authors.initials: ZP
authors.lastname: Xie
authors.forename: K-L
authors.initials: KL
authors.lastname: Yan
authors.forename: H-C
authors.initials: HC
authors.lastname: Gao
authors.forename: J-R
authors.initials: JR
authors.lastname: Song
authors.forename: X-H
authors.initials: XH
authors.lastname: Yuan
authors.forename: W-X
authors.initials: WX
authors.lastname: Cheng
authors.forename: Y-D
authors.initials: YD
authors.lastname: Hou
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002681:China; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D014777:Virus Diseases / Q000821:virology*; D014780:Viruses / Q000302:isolation & purification / Q000472:pathogenicity*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral