title: Comparative analysis of gelatin scaffolds crosslinked by genipin and silane coupling agent.
abstract: Scaffolds based on gelatin (G) are considered promising for tissue engineering, able to mimic the natural extracellular matrix. G drawback is its poor structural consistency in wet conditions. Therefore, crosslinking is necessary to fabricate stable G scaffolds. In this work, a comparative study between the performance of two different crosslinkers, genipin (GP) and γ-glycidoxypropyltrimethoxysilane (GPTMS), is presented. Flat membranes by solvent casting and porous crosslinked scaffolds by freeze-drying were prepared. Infrared spectroscopy and thermal analysis were applied to confirm G chain crosslinking. Moreover, GP and GPTMS increased the stability of G in aqueous media and improved the mechanical properties. Crosslinking reduced the wettability, especially in the case of G_GPTMS samples, due to the introduction of hydrophobic siloxane chains. Both G_GP and G_GPTMS scaffolds supported MG-63 osteoblast-like cell adhesion and proliferation.
authors.affiliation: Department of Mechanics, Politecnico di Torino, Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tonda-Turo
authors.forename: P
authors.initials: P
authors.lastname: Gentile
authors.forename: S
authors.initials: S
authors.lastname: Saracino
authors.forename: V
authors.initials: V
authors.lastname: Chiono
authors.forename: V K
authors.initials: VK
authors.lastname: Nandagiri
authors.forename: G
authors.initials: G
authors.lastname: Muzio
authors.forename: R A
authors.initials: RA
authors.lastname: Canuto
authors.forename: G
authors.initials: G
authors.lastname: Ciardelli
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002452:Cell Count; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000737:chemistry*; D055119:Elastic Modulus; D005780:Gelatin / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D057889:Iridoid Glycosides / Q000737:chemistry*; D039823:Iridoids; D008422:Materials Testing; D016062:Porosity; D012821:Silanes / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*; D017155:Wettability
chemical_list: D003432:Cross-Linking Reagents; D057889:Iridoid Glycosides; D039823:Iridoids; D012821:Silanes; C039462:gamma-glycidoxypropyltrimethoxysilane; D005780:Gelatin; C007834:genipin