title: Effect of desensitizing agents on the microtensile bond strength of a two-step self-etch adhesive to dentin.
abstract: The aim of this in vitro study was to evaluate the effect of cervical hypersensitivity treatments (neodymium yttrium aluminum garnet [Nd:YAG] laser and conventional techniques) on the microtensile bond strengths of adhesives to treated dentin. The buccal cervical enamel of 42 freshly extracted human mandibular third molars was ground flat to expose the cervical dentin. The dentin surfaces were polished with a series of silicon carbide papers, and the smear was removed with an ethylenediamine tetra-acetic acid solution. The teeth were randomly divided into six groups as follows: group 1, Vivasens; group 2, BisBlock; group 3, fluoride gel; group 4, Nd:YAG laser; group 5, Clearfil SE + Nd:YAG laser; and group 6, no treatment (control). The specimens were then restored with a two-step self-etch adhesive, with the exception of group 5. Five specimens from each group were restored with a nanohybrid composite resin. The adhesive interface of two specimens from each group was examined using scanning electron microscopy. The specimens were sectioned perpendicularly to the adhesive interface to produce beams (adhesive area 1 mm(2)). The beams were then attached to a microtensile tester and stressed to failure at 1 mm/min. The data were compared using one-way analysis of variance at a significance level of 0.05. The microtensile bond strengths of the control group were significantly higher than those found for group 1, group 2, group 3, and group 4 (p< 0.05). No significant difference was found between group 5 and the control group. Most of the premature failures were seen in group 2 (80%), and the fewest premature failures were seen in group 5 (13.3%). The SEM findings verified the microtensile test findings. In conclusion, desensitizing treatment procedures (with the exception of Clearfil SE + Nd:YAG laser) reduced the microtensile bond strength of a two-step self-etch adhesive to dentin.
authors.affiliation: Gazi University Faculty of Dentistry, Operative Dentistry and Endodontics, Ankara, Turkey.
authors.forename: Hacer Deniz
authors.initials: HD
authors.lastname: Arisu
authors.forename: Evrim
authors.initials: E
authors.lastname: Dalkihç
authors.forename: Mine Betül
authors.initials: MB
authors.lastname: Üçtaşli
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003764:Dental Materials / Q000737:chemistry; D003793:Dental Restoration, Permanent / Q000379:methods; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000528:radiation effects / Q000648:ultrastructure*; D057133:Dentin Desensitizing Agents / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D005459:Fluorides / Q000737:chemistry; D006801:Humans; D053844:Lasers, Solid-State / Q000627:therapeutic use; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry; D019815:Oxalic Acid / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D016369:Smear Layer; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D019237:Tooth Cervix / Q000528:radiation effects / Q000648:ultrastructure
chemical_list: C514542:BisBlock; D002614:Chelating Agents; C438313:Clearfil SE Bond; D003188:Composite Resins; D003764:Dental Materials; D057133:Dentin Desensitizing Agents; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C525245:VivaSens Liner; D019815:Oxalic Acid; D004492:Edetic Acid; D005459:Fluorides