title: Metabolite profiling of recombinant CHO cells: designing tailored feeding regimes that enhance recombinant antibody production.
abstract: Chinese hamster ovary (CHO) cells are the primary platform for commercial expression of recombinant therapeutic proteins. Obtaining maximum production from the expression platform requires optimal cell culture medium (and associated nutrient feeds). We have used metabolite profiling to define the balance of intracellular and extracellular metabolites during the production process of a CHO cell line expressing a recombinant IgG4 antibody. Using this metabolite profiling approach, it was possible to identify nutrient limitations, which acted as bottlenecks for antibody production, and subsequently develop a simple feeding regime to relieve these metabolic bottlenecks. This metabolite profiling-based strategy was used to design a targeted, low cost nutrient feed that increased cell biomass by 35% and doubled the antibody titer. This approach, with the potential for utilization in non-specialized laboratories, can be applied universally to the optimization of production of commercially important biopharmaceuticals.
authors.affiliation: Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Sellick
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Croxford
authors.forename: Arfa R
authors.initials: AR
authors.lastname: Maqsood
authors.forename: Gill
authors.initials: G
authors.lastname: Stephens
authors.forename: Hans V
authors.initials: HV
authors.lastname: Westerhoff
authors.forename: Royston
authors.initials: R
authors.lastname: Goodacre
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Dickson
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000737:chemistry* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000737:chemistry*; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D055442:Metabolome; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013678:Technology, Pharmaceutical / Q000379:methods
chemical_list: D003470:Culture Media; D007074:Immunoglobulin G; D011994:Recombinant Proteins