title: Calcium sulfate stimulates pulp stem cells towards osteoblasts differentiation.
abstract: Calcium sulfate (CaS) is a highly biocompatible material and enhances bone formation in vivo. However, how CaS alters osteoblast activity to promote bone formation is poorly understood. To study how CaS can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were compared in normal osteoblasts and dental pulp stem cells, using real time Reverse Transcription-Polymerase Chain Reaction. Gene differentially expressed between the two cells type were the trascriptional factor RUNX2, osteopontin (SPP1), COL1A1 (collagen type 1α1) and alkaline phosphatase (ALPL). The obtained results demonstrated that CaS strongly influences the behavior of DPSCs in vitro enhancing proliferation, differentiation and deposition of matrix.
authors.affiliation: Orthopedic Clinic, University of Ferrara, Ferrara, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Sollazzo
authors.forename: A
authors.initials: A
authors.lastname: Lucchese
authors.forename: A
authors.initials: A
authors.lastname: Palmieri
authors.forename: G
authors.initials: G
authors.lastname: Carnevali
authors.forename: C
authors.initials: C
authors.lastname: Iaccarino
authors.forename: I
authors.initials: I
authors.lastname: Zollino
authors.forename: M
authors.initials: M
authors.lastname: Della Valle
authors.forename: F
authors.initials: F
authors.lastname: Pezzetti
authors.forename: G
authors.initials: G
authors.lastname: Brunelli
authors.forename: F
authors.initials: F
authors.lastname: Carinci
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000235:genetics; D002133:Calcium Sulfate / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D053495:Osteopontin / Q000235:genetics; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D055815:Young Adult
chemical_list: D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; C506895:SPP1 protein, human; D053495:Osteopontin; C548267:ALPL protein, human; D000469:Alkaline Phosphatase; D002133:Calcium Sulfate