title: The analysis of the immobilization mechanism of Ni(II) on Bacillus cereus.
abstract: This work focused on the identification of biosorption mechanism of Ni(II) by living Bacillus cereus (B. cereus) based on batch experiments and a variety of microscopic equipments. The adsorption equilibrium reached rapidly in 2 h and the maximum nickel adsorption capability of B. cereus was 17.7 mg x g(-1) (dry weight). Atomic force microscopy (AFM) analysis showed that the bacterial surface roughness increased from 7.9 +/- 0.5 nm to 12.6 +/- 1.6 nm during this process. Scanning electron microscopy (SEM) observation confirmed that there was Ni(II) on the bacterial surface. However, transmission electron microscopy (TEM) thin section analysis coupled with energy dispersive X-ray spectroscopy (EDS) revealed that Ni(II) could also be found in the inner portions of the bacteria. Inductive coupled plasma emission spectrometer (ICP-OES) quantitative analysis elucidated that over 70% of the immobilized Ni(II) was binding on the surface of bacteria. X-ray diffraction (XRD) analysis showed that the Ni(II) collected by the bacteria was amorphous. Fourier transform infrared (FT-IR) analysis indicated that amides and carboxylation functional groups might be involved in the coordination of Ni(II).
authors.affiliation: Key Lab of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Ministry of Education, Fuzhou, Fujian 350002, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Pan
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Yangjian
authors.initials: Y
authors.lastname: Cheng
authors.forename: Danmei
authors.initials: D
authors.lastname: Pan
authors.forename: Shungao
authors.initials: S
authors.lastname: Yin
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Xiong
authors.initials: X
authors.lastname: Guan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lin
mesh_terms: D000327:Adsorption; D001409:Bacillus cereus / Q000737:chemistry*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D009532:Nickel / Q000737:chemistry*; D010316:Particle Size
chemical_list: D009532:Nickel