title: [Identification of the lignans components after oral administration of fuzheng huayu decoction in rat serum by HPLC-MSn].
abstract: OBJECTIVE
  To study the lignans components in rat serum after oral administration of Fuzheng Huayu decoction (FZHY), and to investigate the active ingredients in vivo.
  
  
  METHODS
  A rapid, sensitive and selective method using liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MSn) was established. The serum samples were extracted with ethyl acetate (EtOAc)for three times. The chromatographic separation was achieved on a Waters Atlantis T3 column by gradient elution using methanol and water containing 5 mmol x L(-1) ammonium acetate as mobile phase, at a flow rate of 0.2 mL x min(-1). Mass spectra were acquired in positive ion mode. Identification and structural elucidation of the components in FZHY and dosed serum were performed by comparing their retention time and MSn spectra with those of reference compounds and reported data in the literatures.
  
  
  RESULTS
  Schisandrin, schisandrol B, schisantherin A and schisandrin B were found in FZHY and dosed serum, but schisandrin C and deoxyschizandrin were only found in FZHY.
  
  
  CONCLUSIONS
  Schisandrin, schisandrol B, schisantherin A and schisandrin B can be directly absorbed into the blood after oral administration of FZHY, and the four lignans components from Schisandra chineisis might play a key role as the ingredient basement of FZHY for anti-liver fibrosis.
authors.affiliation: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Danping
authors.initials: D
authors.lastname: Shen
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tao
authors.forename: Qinglan
authors.initials: Q
authors.lastname: Wang
authors.forename: Chenghai
authors.initials: C
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D017705:Lignans / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal; D017705:Lignans; C532829:fuzheng huayu