title: Genome-wide expression profiling of hepatic oval cells after partial hepatectomy in rats.
abstract: To examine the changes of biological activities in hepatic oval cells (HOCs) elicited by 70% partial hepatectomy (PH) and understand the relationship between this cell and liver regeneration (LR), this study isolated and obtained the high purity HOCs (≥ 95%) from rat regenerating livers, and then monitored gene expression profiling of rat hepatic oval cells following surgical operation. Results showed that there were LR-related 1059 genes. These genes were grossly classified into three groups using a fold change cut-off threshold of three-fold: up-regulation, down-regulation and up/down regulation. Analyses of gene expression patterns combined with gene functional categorization suggested that genes in the categories "nucleic acid metabolism" and "cell cycle" were dominated by up-regulated expression. Genes in the functional groups "cell metabolism" and "oxidation reduction" were significantly enriched in expression pattern characterized by down-regulation. According to above mentioned results, the synchronized induction of DNA replication and cell proliferation-involved genes suggested that the peak of oval cell proliferation might occur between 30 and 36 h post-PH. The amino acid transformation-involved genes were down-regulated at the early phase of LR, which perhaps trigger the storage of those amino acids essential for protein synthesis. Reduced oxidative-reduction activity at early phase might be related to negative influence of surgical operation on its detoxification capacity. Conclusively, the genome-wide transcriptional analysis of oval cells would contribute to our understanding of the nature of LR at cell level.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, China. cellkeylab@126.com
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.forename: Cuifang
authors.initials: C
authors.lastname: Chang
authors.forename: Gaiping
authors.initials: G
authors.lastname: Wang
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.forename: Lianxing
authors.initials: L
authors.lastname: Zhang
authors.forename: Qiushi
authors.initials: Q
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Fuchun
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D016000:Cluster Analysis; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology* / Q000502:physiology; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000502:physiology; D008115:Liver Regeneration; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins