title: Transcriptomic, proteomic and metabolomic analysis of maize responses to UV-B: comparison of greenhouse and field growth conditions.
abstract: UV-B radiation from normal solar fluence elicits physiological and developmental changes in plants under fluctuating environmental conditions. Most UV photobiology studies in plants utilize controlled greenhouse and growth chamber environments in which few conditions vary except the brief presence of UV-B radiation. Our purpose was to compare responses to UV-B in irradiated and shielded maize organs in field (natural solar plus 2x solar supplementation for defined periods) and greenhouse (2x solar supplementation only) conditions during a 4 hour exposure. Three parameters were assessed--transcripts, proteins, and metabolites--to determine the degree of overlap in maize responses in field and greenhouse conditions. We assessed irradiated leaves, and both shielded leaves and immature ears. After comparing transcriptome, proteome and metabolome profiles, we find there are more differences than similarities between field and greenhouse responses.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, Rosario, Argentina.
authors.forename: Paula
authors.initials: P
authors.lastname: Casati
authors.forename: Mabel
authors.initials: M
authors.lastname: Campi
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Morrow
authors.forename: John
authors.initials: J
authors.lastname: Fernandes
authors.forename: Virginia
authors.initials: V
authors.lastname: Walbot
mesh_terms: D055442:Metabolome; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D020543:Proteome / Q000032:analysis*; D059467:Transcriptome; D014466:Ultraviolet Rays; D003313:Zea mays / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D020543:Proteome