title: Stability, antimicrobial activity, and cytotoxicity of poly(amidoamine) dendrimers on titanium substrates.
abstract: In this article, we present the first report on the antibacterial activity and cytotoxicity of poly(amidoamine) (PAMAM) dendrimers immobilized on three types of titanium-based substrates with and without calcium phosphate coating. We show that the amino-terminated PAMAM dendrimers modified with various percentages (0-60%) of poly(ethylene glycol) (PEG) strongly adsorbed on the titanium-based substrates. The resultant dendrimer films effectively inhibited the colonization of the Gram-negative bacteria Pseudomonas aeruginosa (strain PAO1) and, to a lesser extent, the Gram-positive bacteria Staphylococcus aureus (SA). The antibacterial activity of the films was maintained even after storage of the samples in PBS for up to 30 days. In addition, the dendrimer films had a low cytotoxicity to human bone mesenchymal stem cells (hMSCs) and did not alter the osteoblast gene expression promoted by the calcium phosphate coating.
authors.affiliation: Biomaterials Research Center, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Uriel J
authors.initials: UJ
authors.lastname: Erasquin
authors.forename: Meirong
authors.initials: M
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.forename: Martin Yi
authors.initials: MY
authors.lastname: Zhang
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Cheng
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Wang
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Cai
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry* / Q000633:toxicity; D002130:Calcium Phosphates / Q000737:chemistry; D002478:Cells, Cultured; D050091:Dendrimers / Q000737:chemistry* / Q000633:toxicity; D005786:Gene Expression Regulation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D002130:Calcium Phosphates; D050091:Dendrimers; C104700:PAMAM Starburst; C020243:calcium phosphate; D014025:Titanium