title: Gene expression profiling in respond to TBT exposure in small abalone Haliotis diversicolor.
abstract: In this study, we investigated the gene expression profiling of small abalone, Haliotis diversicolor by tributyltin (TBT) exposure using a cDNA microarray containing 2473 unique transcripts. Totally, 107 up-regulated genes and 41 down-regulated genes were found. For further investigation of candidate genes from microarray data and EST analysis, quantitative real-time PCR was performed at 6 h, 24 h, 48 h, 96 h and 192 h TBT exposure. 26 genes were found to be significantly differentially expressed in different time course, 3 of them were unknown. Some gene homologues like cellulose, endo-beta-1,4-glucanase, ferritin subunit 1 and thiolester containing protein II CG7052-PB might be the good biomarker candidate for TBT monitor. The identification of stress response genes and their expression profiles will permit detailed investigation of the defense responses of small abalone genes.
authors.affiliation: The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian 361021, China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: Jia
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zou
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.forename: Shuhong
authors.initials: S
authors.lastname: Wang
authors.forename: Yilei
authors.initials: Y
authors.lastname: Wang
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D020224:Expressed Sequence Tags; D049851:Gastropoda / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin