title: Gene expression using the PALM system.
abstract: The PALM Robot MicroBeam laser microdissection system can isolate specified cells from complex tissues section, in a rapid and precise manner. Combined with other methods, PALM may be used for gene expression elucidating the role of specialized cell type in physiological and pathological activity. This chapter describes the application of the PALM MicroBeam system to isolate RNA from cells in a complex tissue for subsequent gene expression analysis. Protocols show the steps from preparation of tissue samples to the final quantitative results. The process is articulated in several steps, each of which requires optimal choices in order to obtain reliable data from a limited number of cells (500-10,000 cells). Furthermore, the notes regarding tissue preparation, microdissection of the interested cells, are also emphasized.
authors.affiliation: Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Lu
authors.forename: Cheuk-Chun
authors.initials: CC
authors.lastname: Szeto
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000295:instrumentation* / Q000379:methods; D008867:Microtomy / Q000379:methods; D009399:Nephrons / Q000166:cytology; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D012371:Robotics; D013194:Staining and Labeling / Q000379:methods
chemical_list: D012313:RNA