title: Effect of 1,25(OH)(2)D(3) on rat peritoneal mesothelial cells treated with high glucose plus lipopolysaccharide.
abstract: 1,25(OH)(2)D(3), the active metabolite of vitamin D(3), its activity is not limited to mineral and skeletal homeostasis. In recent years, there has been increasing evidence pointing to the role of its activity in the regulation of cell proliferation, cell differentiation and immunomodulation. Here we report lipopolysaccharide (LPS), a glycolipid that is produced and secreted by gram-negative bacteria during peritonitis, plus high glucose (HG) can significantly inhibit mesothelial cell viability while induce more apoptosis in rat peritoneal mesothelial cells (RPMC). Pretreatment with 1,25(OH)(2)D(3) can reverse the above effect in a concentration dependent manner. HG plus LPS can down-regulate the levels of both mRNA and protein of VDR, and up-regulate the expression of TGF-β1 and TNF-α in RPMC, which can also be effectively reversed by pretreatment with 1,25(OH)(2)D(3). The above results suggest that HG plus LPS may induce changes in RPMC's viability and apoptosis, leading to peritoneal injury. 1,25(OH)(2)D(3) can reverse the inhibition of cell viability, the increase of apoptotic rate and induction of fibrosis related cytokine TGF-β1 and TNF-α by HG plus LPS in RPMC, thus protect peritoneal membrane.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.forename: Lining
authors.initials: L
authors.lastname: Wang
authors.forename: Jianfei
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002117:Calcitriol / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects*; D005947:Glucose / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology
chemical_list: D008070:Lipopolysaccharides; D018167:Receptors, Calcitriol; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D014815:Vitamins; D002117:Calcitriol; D005947:Glucose