title: Biochemical study of dermatan sulfate glycosaminoglycan in adult male patients with Nyhus type II inguinal hernia.
abstract: OBJECTIVE
  To compare the amount of the dermatan sulfate glycosaminoglycan between male patients with Nyhus type II inguinal hernias and subjects without inguinal hernia, aged between 20 and 40 years.
  
  
  METHODS
  Two groups were formed: One with 15 male patients with Nyhus type II inguinal hernia and aged between 20 and 40 years with ASA risk I and II, and a control group of ten individuals, also males between 20 and 40, who had died up to 24 h before. We excluded female patients, diabetic patients with connective tissue disease, smokers and surgical risk ASA III and IV. We resected a sample of 1 cm² of the transversalis fascia in the middle of the inguinal trigone, and 1 cm² of the anterior sheath of the rectus abdominis muscle in the groin for the quantification of dermatan sulfate glycosaminoglycans by densitometry after agarose gel electrophoresis.
  
  
  RESULTS
  The amount of dermatan sulfate showed no statistically significant difference between patients with inguinal hernia and individuals without inguinal hernia in both the transverse fascia (p = 0.108) and anterior sheath of the rectus abdominis muscle (p = 0.292).
  
  
  CONCLUSIONS
  There was no difference in the amount of the dermatan sulfate glycosaminoglycan among patients with Nyhus type II inguinal hernias and subjects without inguinal hernia in adult males.
authors.affiliation: Department of Anatomy, UNIFOA-Volta Redonda-RJ, Brazil. evandro.cir@uol.com.br
authors.forename: Evandro de Moraes
authors.initials: Ede M
authors.lastname: e Silva
authors.forename: Gaspar de Jesus
authors.initials: Gde J
authors.lastname: Lopes Filho
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Rogério de Oliveira
authors.initials: Rde O
authors.lastname: Gonçalves
authors.forename: Elza Yoko
authors.initials: EY
authors.lastname: Kobayashi
authors.forename: Juliana Luporini
authors.initials: JL
authors.lastname: Dreyfuss
mesh_terms: D000328:Adult; D003871:Dermatan Sulfate / Q000032:analysis*; D005205:Fascia / Q000737:chemistry*; D006552:Hernia, Inguinal / Q000145:classification*; D006801:Humans; D008297:Male; D017568:Rectus Abdominis / Q000737:chemistry*; D055815:Young Adult
chemical_list: D003871:Dermatan Sulfate