title: Qualitative and quantitative evaluation of phenolic compounds in Iris dichotoma Pall.
abstract: BACKGROUND
  The rhizome of Iris dichotoma Pall., a traditional Chinese herbal medicine that contains (iso) flavonoids as the main bioactive compound, has been used to treat several disorders such as inflammation, throat disorders, asthma and coughs. However, there is a lack of suitable methods for its qualitative and quantitative analysis as well as quality control.
  
  
  OBJECTIVE
  To establish an HPLC method for the simultaneous determination of phenolic constituents, the identification of characteristic metabolites in the rhizomes of I. dichotoma and to compare their contents in samples from different areas of China.
  
  
  METHODS
  HPLC coupled with diode-array detection (DAD) and electrospray ionisation multistage mass spectrometry (ESI-MS(n) ), and principal component analysis (PCA) were used for the qualitative and quantitative analysis of phenolic compounds in I. dichotoma samples.
  
  
  RESULTS
  Twenty characteristic compounds were identified or tentatively characterised. A quantitative HPLC-UV method allowing the simultaneous quantification of 10 phenolic compounds was optimised and validated in terms of linearity, precision, accuracy, and limits of detection and quantification.
  
  
  CONCLUSIONS
  The methodology developed proved to be effective for the identification and quantification of phenolic compounds in the rhizomes of I. dichotoma. Samples were classified into three groups according to their geographical origin based on their phenolic composition using principal component analysis.
authors.affiliation: Department of Resources Science of Traditional Chinese Medicines, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wei
authors.forename: Pan
authors.initials: P
authors.lastname: Shu
authors.forename: Junli
authors.initials: J
authors.lastname: Hong
authors.forename: Minjian
authors.initials: M
authors.lastname: Qin
mesh_terms: D002138:Calibration; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D005843:Geography; D006027:Glycosides / Q000032:analysis / Q000737:chemistry; D031323:Iris Plant / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D025341:Principal Component Analysis; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D007529:Isoflavones; D010636:Phenols