title: [Gene RAD31 is identical to gene MEC1 of yeast Saccharomyces cerevisiae].
abstract: The earlier identified gene RAD31 was mapped on the right arm of chromosome II in the region of gene MEC1 localization. Epistatic analysis demonstrated that the rad31 mutation is an allele of the MEC1 gene, which allows further designation of the rad31 mutation as mec1-212. Mutation mec1-212, similar to deletion alleles of this gene, causes sensitivity to hydroxyurea, disturbs the check-point function, and suppresses UV-induced mutagenesis. However, this mutation significantly increases the frequency of spontaneous canavanine-resistance mutations induced by disturbances in correcting errors of DNA replication and repair, which distinguishes it from all identified alleles of gene MEC1.
authors.forename: T N
authors.initials: TN
authors.lastname: Kozhina
authors.forename: S A
authors.initials: SA
authors.lastname: Kozhin
authors.forename: V G
authors.initials: VG
authors.lastname: Korolev
mesh_terms: D000483:Alleles; D002172:Canavanine / Q000235:genetics; D002874:Chromosome Mapping; D004843:Epistasis, Genetic; D018816:Genes, cdc; D006918:Hydroxyurea / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D016296:Mutagenesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D017384:Sequence Deletion; D014466:Ultraviolet Rays
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D002172:Canavanine; C088937:MEC1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D006918:Hydroxyurea