title: Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1.
abstract: Enhancers of transcription activate transcription via binding of sequence-specific transcription factors to their target sites in chromatin. In this report, we identify GATA1-bound distal sites genome-wide and find a global reorganization of the nucleosomes at these potential enhancers during differentiation of hematopoietic stem cells (HSCs) to erythrocytes. We show that the catalytic subunit BRG1 of BAF complexes localizes to these distal sites during differentiation and generates a longer nucleosome linker region surrounding the GATA1 sites by shifting the flanking nucleosomes away. Intriguingly, we find that the nucleosome shifting specifically facilitates binding of TAL1 but not GATA1 and is linked to subsequent transcriptional regulation of target genes.
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Gangqing
authors.initials: G
authors.lastname: Hu
authors.forename: Dustin E
authors.initials: DE
authors.lastname: Schones
authors.forename: Kairong
authors.initials: K
authors.lastname: Cui
authors.forename: River
authors.initials: R
authors.lastname: Ybarra
authors.forename: Daniel
authors.initials: D
authors.lastname: Northrup
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Tang
authors.forename: Luca
authors.initials: L
authors.lastname: Gattinoni
authors.forename: Nicholas P
authors.initials: NP
authors.lastname: Restifo
authors.forename: Suming
authors.initials: S
authors.lastname: Huang
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D001665:Binding Sites; D000076246:CCCTC-Binding Factor; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly; D047369:Chromatin Immunoprecipitation; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D050982:GATA1 Transcription Factor / Q000378:metabolism*; D005786:Gene Expression Regulation; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D009707:Nucleosomes; D009928:Organ Specificity; D061251:Primary Cell Culture; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D000076246:CCCTC-Binding Factor; D004265:DNA Helicases; D050982:GATA1 Transcription Factor; D009687:Nuclear Proteins; D009707:Nucleosomes; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; C000619697:CTCF protein, human; C494688:GATA1 protein, human; C067143:TAL1 protein, human; C084108:SMARCA4 protein, human