title: Improvement of dermal burn healing by combining sodium alginate/chitosan-based films and low level laser therapy.
abstract: This paper aimed to evaluate the improvement of burn wounds healing by sodium alginate/chitosan-based films and laser therapy. Natural polymers with different biological activities are widely used as film dressings to improve wound healing. Lasers arrays accelerate the healing repair of soft tissue injuries. Burn procedures were performed on the backs of 60 male rats assigned into six groups: untreated (CTR), dressed with cellulose films (CL), dressed with sodium alginate/chitosan-based films (SC), laser-irradiated undressed wounds (LT), laser-irradiated wounds with cellulose (CLLT) and sodium alginate/chitosan-based films (SCLT). Laser therapy was applied for 7 days. Animals of each group were euthanised 8 and 14 days after the burn procedures. The inflammatory reaction was significantly more intense in the CTR group than in the irradiated groups after 8 and 14 days. Laser therapy stimulated myofibroblastic differentiation in 8 days, with or without dressing films. Combined laser therapy and both dressings improved epithelisation, blood vessels formation and collagenization, promoted rapid replacement of type III for type I collagen and favored the better arrangement of the newly formed collagen fibres. The combination of laser therapy and sodium alginate/chitosan-based dressing improves burn healing, apparently by modulating the epithelisation, blood vessels formation and collagenization processes.
authors.affiliation: Tiradentes University, Av. Murilo Dantas, 300, Farolândia, CEP 49030-490, Aracaju/SE, Brazil. marxdantas@hotmail.com
authors.forename: M D M
authors.initials: MD
authors.lastname: Dantas
authors.forename: D R R
authors.initials: DR
authors.lastname: Cavalcante
authors.forename: F E N
authors.initials: FE
authors.lastname: Araújo
authors.forename: S R
authors.initials: SR
authors.lastname: Barretto
authors.forename: G T S
authors.initials: GT
authors.lastname: Aciole
authors.forename: A L B
authors.initials: AL
authors.lastname: Pinheiro
authors.forename: M A G
authors.initials: MA
authors.lastname: Ribeiro
authors.forename: I B
authors.initials: IB
authors.lastname: Lima-Verde
authors.forename: C M
authors.initials: CM
authors.lastname: Melo
authors.forename: J C
authors.initials: JC
authors.lastname: Cardoso
authors.forename: R L C
authors.initials: RL
authors.lastname: Albuquerque Júnior
mesh_terms: D000199:Actins / Q000378:metabolism; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D002056:Burns / Q000473:pathology / Q000532:radiotherapy*; D048271:Chitosan / Q000494:pharmacology*; D024042:Collagen Type I / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D028022:Low-Level Light Therapy; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000528:radiation effects; D051381:Rats; D014945:Wound Healing / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000199:Actins; D000464:Alginates; D048271:Chitosan; D024042:Collagen Type I; D020723:Glucuronic Acid; D006603:Hexuronic Acids; C498862:Acta2 protein, rat