title: [Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain].
abstract: A heterologous gene expression system was created in a domestic Aspergillus awamori Co-6804 strain, which is a producer of the glucoamylase gene. Vector pGa was prepared using promoter and terminator areas of the glucoamylase gene, and A. niger phytase, Trichoderma reesei endoglucanase, and Penicillium canescens xylanase genes were then cloned into pGa vector. Separation of enzyme samples using FPLC showed the amount of the recombinant proteins to be within the 0.6-14% range of total protein.
authors.forename: A M
authors.initials: AM
authors.lastname: Rozhkova
authors.forename: A S
authors.initials: AS
authors.lastname: Sereda
authors.forename: N V
authors.initials: NV
authors.lastname: Tsurikova
authors.forename: A K
authors.initials: AK
authors.lastname: Nurtaeva
authors.forename: M V
authors.initials: MV
authors.lastname: Semenova
authors.forename: L V
authors.initials: LV
authors.lastname: Rimareva
authors.forename: E A
authors.initials: EA
authors.lastname: Rubtsova
authors.forename: I N
authors.initials: IN
authors.lastname: Zorov
authors.forename: O A
authors.initials: OA
authors.lastname: Sinitsyna
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
mesh_terms: D010832:6-Phytase / Q000235:genetics / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D001709:Biotechnology; D002480:Cellulase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000737:chemistry* / Q000302:isolation & purification*; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013728:Terminator Regions, Genetic; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D010832:6-Phytase; D005087:Glucan 1,4-alpha-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase