title: Characterization of cytochrome c folding intermediates induced by sucrose and phosphate.
abstract: Spectroscopic and calorimetric investigations of the folding of denatured cytochrome c in the presence of phosphate ion and sugar were carried out to understand subtle differences in the nature of induced conformation and folding energy landscape. Altered conformations of cyt c induced by sucrose and phosphate, with same absorbance wavelength maxima, exhibit lack of tertiary interactions in segment 70-85 and similar α-helical content. However, compactness, the exposure of the heme to solvent and the secondary structure content in the two conformations are different. Although downhill folding was observed for both conformations, extent of cooperativity is higher in case of phosphate-induced conformation.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Delhi (IIT Delhi), Hauzkhas, New Delhi, India.
authors.forename: Unnati
authors.initials: U
authors.lastname: Ahluwalia
authors.forename: Chetan
authors.initials: C
authors.lastname: Prakash
authors.forename: Rakhi
authors.initials: R
authors.lastname: Agrawal
authors.forename: Shashank
authors.initials: S
authors.lastname: Deep
mesh_terms: D000143:Acids; D000818:Animals; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry* / Q000378:metabolism*; D006736:Horses; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017510:Protein Folding / Q000187:drug effects*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013395:Sucrose / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D000143:Acids; D010710:Phosphates; D012996:Solutions; D013395:Sucrose; D045304:Cytochromes c; D009195:Peroxidase