title: Unique heparan sulfate from shrimp heads exhibits a strong inhibitory effect on infections by dengue virus and Japanese encephalitis virus.
abstract: The structure and biological activities of a highly sulfated heparan sulfate (HS) extracted from shrimp (Penaeus brasiliensis) heads were characterized. Structurally the shrimp HS was more heterogenous than heparin, although it is still highly sulfated. The molecular mass of the shrimp HS preparation was determined to be 32.3 kDa by gel filtration HPLC. Analysis by surface plasmon resonance demonstrated that various growth/differentiation factors specifically bound to the shrimp HS with comparable affinity. Notably, the shrimp HS had a greater inhibitory effect against infections by dengue virus type 2 as well as Japanese encephalitis virus than heparin. Experiments on anticoagulant activity indicated that the shrimp HS exhibited significant anti-thrombin activity, but less than the commercial heparin. Hence, the HS preparation from shrimp heads, an industrial waste, is a prospective agent for a variety of clinical applications.
authors.affiliation: Laboratory of Proteoglycan Signaling and Therapeutics, Hokkaido University Graduate School of Life Science, Sapporo 001-0021, Japan.
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Chen
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamada
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hama
authors.forename: Ajaya Kumar
authors.initials: AK
authors.lastname: Shetty
authors.forename: Takanari
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oda
authors.forename: Kosuke
authors.initials: K
authors.lastname: Seiki
authors.forename: Eunmi
authors.initials: E
authors.lastname: Kim
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
authors.forename: Naonori
authors.initials: N
authors.lastname: Takahashi
authors.forename: Kazuya I P J
authors.initials: KI
authors.lastname: Hidari
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Sugahara
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003716:Dengue Virus / Q000187:drug effects*; D004664:Encephalitis Virus, Japanese / Q000187:drug effects*; D006497:Heparan Sulfate / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D033561:Penaeidae / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D000998:Antiviral Agents; D006497:Heparan Sulfate