title: Detection of novel human MiRNAs responding to X-ray irradiation.
abstract: Up to now, more than 1048 human miRNAs have been identified. However, the recognition of new human miRNAs is becoming more and more difficult. Based on the hypothesis that the expression of some miRNAs can be induced by ionizing radiation, total RNAs of HeLa cells were isolated 1 h after exposure to 2 Gy of X-rays, and total small RNAs were enriched and sequenced by PAGE and Solexa technology, respectively. As a result, 421 kinds of known miRNAs and 337 kinds of unknown sequences were identified, among which 10 novel miRNAs were characterized by bioinformatic approach and verified by qRT-PCR. Finally, putative targets of these miRNAs were predicted by TargetScan software and compared with known proteins down-regulated by radiation. It was confirmed that some of the targets of these novel miRNAs were radiation-related proteins. These results imply that these 10 novel miRNAs are radiation-related miRNAs. This study reveals a new way to find novel miRNAs.
authors.affiliation: Institute of Modern Physics, Chinese Academy of Sciences.
authors.forename: Nan
authors.initials: N
authors.lastname: Ding
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Jinpeng
authors.initials: J
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Chang
authors.forename: Wentao
authors.initials: W
authors.lastname: Hu
authors.forename: Wenjian
authors.initials: W
authors.lastname: Li
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
authors.forename: Tieshan
authors.initials: T
authors.lastname: Wang
authors.forename: Guangming
authors.initials: G
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology / Q000379:methods; D018076:DNA, Complementary / Q000378:metabolism; D005821:Genetic Techniques; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000528:radiation effects*; D008969:Molecular Sequence Data; D012313:RNA / Q000528:radiation effects*; D011839:Radiation, Ionizing; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014965:X-Rays
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; D012313:RNA