title: Detection and quantification of bovine papilloma virus type 2 (BPV-2) by real-time PCR in urine and urinary bladder lesions in enzootic bovine haematuria (EBH)-affected cows.
abstract: This study was conducted with the objectives of detecting bovine papillomavirus type 2 (BPV-2) in urine samples and urinary bladder lesions in bovines using polymerase chain reaction (PCR) and real-time PCR-based molecular diagnostic tests, and quantifying BPV-2 in urinary bladder lesions especially in enzootic bovine haematuria (EBH)-affected animals. BPV-2 viral DNA was detected in urine samples (50%) and urinary bladder tissue (68.6%). Cloning and sequencing results showed a close homology with other Indian BPV-2 sequences. Quantitative real-time PCR (SYBR Green assay) showed that the BPV-2 load was low and similar irrespective of inflammatory or neoplastic lesions in the bladder. It was concluded that BPV-2 DNA is frequently present in urine and urinary bladder lesions in cows in an EBH endemic region and virus load was low in urinary bladder lesions.
authors.affiliation: Division of Pathology, Indian Veterinary Research Institute, Izatnagar, UP, India.
authors.forename: S
authors.initials: S
authors.lastname: Pathania
authors.forename: K
authors.initials: K
authors.lastname: Dhama
authors.forename: G
authors.initials: G
authors.lastname: Saikumar
authors.forename: S
authors.initials: S
authors.lastname: Shahi
authors.forename: R
authors.initials: R
authors.lastname: Somvanshi
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000453:epidemiology / Q000652:urine / Q000821:virology*; D003612:Dairying; D006417:Hematuria / Q000821:virology*; D007194:India / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D001749:Urinary Bladder Neoplasms / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D019562:Viral Load