title: Resveratrol inhibits the epidermal growth factor-induced epithelial mesenchymal transition in MCF-7 cells.
abstract: Carcinoma progression is associated with the loss of epithelial features, and the acquisition of a mesenchymal phenotype by tumour cells. Herein we show that exposure of MCF-7 cells to epidermal growth factor (EGF) resulted in morphological alterations characteristic of epithelial-to-mesenchymal transition (EMT). EGF treatment resulted in increased motility along with an up-regulation of transcription factors Slug, Zeb1, Zeb2, and mesenchymal markers Vimentin and N-cadherin. Treatment of MCF-7 cells with a combined stimulation of EGF and resveratrol, a naturally occurring stilbene with antitumor properties, failed to alter cell morphology, motility and overexpression of EMT markers induced by EGF. Using specific chemical inhibitors, we demonstrated that EGF-induced EMT is mediated by extracellular signal-regulated kinase 1/2 (ERK 1/2) signalling pathway and that resveratrol is able to repress EGF-induced ERK activation. In summary, these data provide new evidence of the inhibitory effect of resveratrol on EGF-induced EMT cell transformation.
authors.affiliation: Laboratory of General Physiology, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Monteroni 73100, Lecce, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Vergara
authors.forename: Concetta Maria
authors.initials: CM
authors.lastname: Valente
authors.forename: Andrea
authors.initials: A
authors.lastname: Tinelli
authors.forename: Carlo
authors.initials: C
authors.lastname: Siciliano
authors.forename: Vito
authors.initials: V
authors.lastname: Lorusso
authors.forename: Raffaele
authors.initials: R
authors.lastname: Acierno
authors.forename: Giovanna
authors.initials: G
authors.lastname: Giovinazzo
authors.forename: Angelo
authors.initials: A
authors.lastname: Santino
authors.forename: Carlo
authors.initials: C
authors.lastname: Storelli
authors.forename: Michele
authors.initials: M
authors.lastname: Maffia
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism; D020533:Angiogenesis Inhibitors / Q000494:pharmacology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002070:Butadienes / Q000494:pharmacology; D015820:Cadherins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D015151:Immunoblotting; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D012097:Repressor Proteins / Q000235:genetics; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071250:Snail Family Transcription Factors; D013267:Stilbenes / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics; D014746:Vimentin / Q000235:genetics; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1
chemical_list: D051339:Actin Depolymerizing Factors; D020533:Angiogenesis Inhibitors; D002070:Butadienes; D015820:Cadherins; D004791:Enzyme Inhibitors; D018398:Homeodomain Proteins; D009570:Nitriles; D012097:Repressor Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D013267:Stilbenes; D014157:Transcription Factors; C113580:U 0126; D014746:Vimentin; C486307:ZEB1 protein, human; C492363:ZEB2 protein, human; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1; D004815:Epidermal Growth Factor; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D000077185:Resveratrol