title: Recombinant CHIK virus E1 coat protein of 11 KDa with antigenic domains for the detection of Chikungunya.
abstract: Chikungunya is an acute febrile illness caused by an alpha virus technically called as CHIK virus. A smaller size of CHIK virus E1 coat protein -11 kDa was expressed in prokaryotic expression system. The recombinant protein was purified and confirmed by western blot analysis. The positions of the antigenic domain in the protein were identified and the immunoreactivity of recombinant protein with anti-CHIK IgM antibodies was ascertained. The antigen showed an 88% sensitivity and 100% specificity by Indirect ELISA. No cross reactivity of the antigen was observed with anti-Dengue virus serum samples. The results strongly support that the recombinant CHIK coat protein could be used as a diagnostic antigen for the detection of Chikungunya by Indirect ELISA. The relevance of a smaller size recombinant antigen highlights its large scale application in serodiagnosis of CHIK virus since bacterial expression is more simple and cost effective than eukaryotic system.
authors.affiliation: SCMS Institute of Bioscience & Biotechnology Research & Development, Management House, South Kalamassery, Cochin, Kerala, PIN-682033, India.
authors.forename: Krishna Kammara
authors.initials: KK
authors.lastname: Yathi
authors.forename: Julia Mary
authors.initials: JM
authors.lastname: Joseph
authors.forename: Salini
authors.initials: S
authors.lastname: Bhasker
authors.forename: Ramesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Mohankumar
authors.initials: M
authors.lastname: Chinnamma
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins