title: Reptile embryology.
abstract: Reptiles (lizards, snakes, turtles and crocodylians) are becoming increasing popular as models for developmental investigations. In this review the leopard gecko, Eublepharis macularius, is presented as a reptilian model for embryonic studies. We provide details of husbandry, breeding and modifications to two popular histological techniques (whole-mount histochemistry and immunohistochemistry). In addition, we provide a summary of basic reptilian husbandry requirements and discuss important details of embryonic nutrition, egg anatomy and sex determination.
authors.affiliation: Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada. mvickary@uoguelph.ca
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Vickaryous
authors.forename: Katherine E
authors.initials: KE
authors.lastname: McLean
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D001947:Breeding; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D004626:Embryology / Q000379:methods*; D005260:Female; D008116:Lizards / Q000196:embryology* / Q000235:genetics; D008297:Male; D016612:Paraffin Embedding; D013194:Staining and Labeling