title: HPLC-UV determination of morphine in human plasma and its application to the clinical study.
abstract: A sensitive and specific high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) has been developed for the quantification of morphine sulfate [(5alpha,6alpha)-7,8-didehydro-4,5-epoxy-17-methylmorphinan-3,6-diol], (CAS: 52-26-6) in human plasma. The analyte was extracted from plasma samples with chloroform - isopropyl alcohol (90:10, v/v) and analyzed on a Bondapak C18 column. The calibration curves were linear within the range of 10-150 ng/mL. The lower limit of quantitation was 10 ng/mL with 0.5 mL plasma sample. The mean recovery of the drug from plasma samples was 83.39%. The results from analysis of quality-control samples at concentrations of 30, 75, and 150 ng/mL were indicative of good accuracy and precision. This method was successfully used to analyze morphine in plasma samples of patients after abdominal hysterectomy.
authors.affiliation: Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, Poland. d.szkutnik@wp.pl
authors.forename: Danuta
authors.initials: D
authors.lastname: Szkutnik-Fiedler
authors.forename: Edmund
authors.initials: E
authors.lastname: Grześkowiak
authors.forename: Michał
authors.initials: M
authors.lastname: Gaca
authors.forename: Magdalena
authors.initials: M
authors.lastname: Borowicz
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D000701:Analgesics, Opioid / Q000097:blood* / Q000627:therapeutic use; D002138:Calibration; D002725:Chloroform / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004355:Drug Stability; D005260:Female; D006801:Humans; D007044:Hysterectomy / Q000009:adverse effects; D008875:Middle Aged; D009020:Morphine / Q000097:blood* / Q000627:therapeutic use; D010149:Postoperative Pain / Q000209:etiology / Q000517:prevention & control; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000592:standards
chemical_list: D000701:Analgesics, Opioid; D012997:Solvents; D009020:Morphine; D002725:Chloroform; D019840:2-Propanol