title: [Lactobacillus acidophilus NCFM affects host adhesion-related gene expression after adhering to host].
abstract: OBJECTIVE
  Adhering to the intestinal epithelial cells is one of the beneficial functions exerting by probiotics. We tested and verified the expression of adhesion-related genes in vivo and in vitro to understand the influence from Lactobacillus acidophilus NCFM on host cells.
  
  
  METHODS
  We selected the adhesion-related genes through GO (gene ontology) category from the Human Genome U133 Plus 2.0 Array analysis. These genes were verified by in vitro Caco-2 cells culture model and in vivo mouse model using Real-time PCR method.
  
  
  RESULTS
  After L. acidophilus NCFM adhering to Caco-2, we found that 12 adhesion-related genes were up-regulated. The up-regulation was confirmed in vivo and in vitro by Real-time PCR assay. Among them, the up-regulated expression of chemokine (C-C motif) ligand 2 (CCL2) gene was the most distinguished one.
  
  
  CONCLUSIONS
  Our research showed that L. acidophilus NCFM, adhering to Caco-2 cells, could cause the differential expression of the host adhesion-related genes. Results of this study seemed to provide some useful data for further revealing its effect on intestinal epithelial cells.
authors.affiliation: Key Lab of Dairy Science, Ministry of Education of China. 359603327@qq.com
authors.forename: Linlin
authors.initials: L
authors.lastname: Han
authors.forename: Chaoxin
authors.initials: C
authors.lastname: Man
authors.forename: Xuena
authors.initials: X
authors.lastname: Lv
authors.forename: Mingna
authors.initials: M
authors.lastname: Wang
authors.forename: Guanghui
authors.initials: G
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yujun
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics* / Q000502:physiology; D018938:Caco-2 Cells; D018932:Chemokine CCL2 / Q000235:genetics*; D004847:Epithelial Cells / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D007779:Lactobacillus acidophilus / Q000502:physiology*; D051379:Mice; D023421:Models, Animal; D012313:RNA / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D012313:RNA