title: Preparative separation and purification of rebaudioside a from steviol glycosides using mixed-mode macroporous adsorption resins.
abstract: Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin-Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40.77 to 60.53%, with a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 96.34% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No. 18 Tianshui Middle Road, Lanzhou 730000, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Duolong
authors.initials: D
authors.lastname: Di
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Bai
authors.forename: Jintian
authors.initials: J
authors.lastname: Li
authors.forename: Zhenbin
authors.initials: Z
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Lou
authors.forename: Helin
authors.initials: H
authors.lastname: Ye
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D045786:Diterpenes, Kaurane / Q000737:chemistry* / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry*; D012117:Resins, Synthetic; D013549:Sweetening Agents; D013816:Thermodynamics
chemical_list: D045786:Diterpenes, Kaurane; D006027:Glycosides; D012117:Resins, Synthetic; D013549:Sweetening Agents; C012042:steviol; C025474:rebaudioside A