title: A class of human proteins that deliver functional proteins into mammalian cells in vitro and in vivo.
abstract: We discovered a class of naturally occurring human proteins with unusually high net positive charge that can potently deliver proteins in functional form into mammalian cells both in vitro and also in murine retina, pancreas, and white adipose tissues in vivo. These findings represent diverse macromolecule delivery agents for in vivo applications, and also raise the possibility that some of these human proteins may penetrate cells as part of their native biological functions.
authors.affiliation: Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Cronican
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Beier
authors.forename: Tina N
authors.initials: TN
authors.lastname: Davis
authors.forename: Jen-Chieh
authors.initials: JC
authors.lastname: Tseng
authors.forename: Weida
authors.initials: W
authors.lastname: Li
authors.forename: David B
authors.initials: DB
authors.lastname: Thompson
authors.forename: Allen F
authors.initials: AF
authors.lastname: Shih
authors.forename: Erin M
authors.initials: EM
authors.lastname: May
authors.forename: Constance L
authors.initials: CL
authors.lastname: Cepko
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Kung
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhou
authors.forename: David R
authors.initials: DR
authors.lastname: Liu
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism*; D030562:Databases, Protein; D006801:Humans; D051379:Mice; D008958:Models, Molecular; D010179:Pancreas / Q000378:metabolism; D021381:Protein Transport; D011506:Proteins / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D012160:Retina / Q000378:metabolism
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins