title: Protection-free one-pot synthesis of 2'-deoxynucleoside 5'-triphosphates and DNA polymerization.
abstract: By differentiating the functional groups on nucleosides, we have designed and developed a one-pot synthesis of deoxyribonucleoside 5'-triphosphates without any protection on the nucleosides. A facile synthesis is achieved by generating an in situ phosphitylating reagent that reacts selectively with the 5'-hydroxyl groups of the unprotected nucleosides. The synthesized triphosphates are of high quality and can be effectively incorporated into DNAs by DNA polymerase. This novel approach is straightforward and cost-effective for triphosphate synthesis.
authors.affiliation: Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
authors.forename: Julianne
authors.initials: J
authors.lastname: Caton-Williams
authors.forename: Matthew
authors.initials: M
authors.lastname: Smith
authors.forename: Nicolas
authors.initials: N
authors.lastname: Carrasco
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
mesh_terms: D004247:DNA / Q000138:chemical synthesis*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry*; D010710:Phosphates / Q000138:chemical synthesis*; D058105:Polymerization
chemical_list: D009705:Nucleosides; D010710:Phosphates; D004247:DNA