title: Quantitative detection of C-deuterated drugs by CARS microscopy and Raman microspectroscopy.
abstract: The introduction of carbon-deuterium (C-D) bonds into drug compounds by organic synthesis is a non-invasive labelling approach, which does not alter the chemical and physiological properties of the drug itself. C-deuterated drugs exhibit characteristic vibrational signatures in the C-D stretching region around 2100-2300 cm(-1), which avoids spectral interference with contributions from a complex biological environment. In this paper, the quantitative detection of C-deuterated drugs by Raman microspectroscopy and single-band CARS microscopy is examined. Concentration-dependent studies on drugs with aliphatic and aromatic C-D moieties were performed in a two-channel microfluidic chip, using the corresponding non-deuterated (C-H) isotopologues as an internal reference.
authors.affiliation: Institute of Photonic Technologies, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: Gero
authors.initials: G
authors.lastname: Bergner
authors.forename: Christian R
authors.initials: CR
authors.lastname: Albert
authors.forename: Markus
authors.initials: M
authors.lastname: Schiller
authors.forename: Gerhard
authors.initials: G
authors.lastname: Bringmann
authors.forename: Tanja
authors.initials: T
authors.lastname: Schirmeister
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dietzek
authors.forename: Stephan
authors.initials: S
authors.lastname: Niebling
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schlücker
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D002244:Carbon / Q000737:chemistry*; D003903:Deuterium / Q000737:chemistry; D004976:Ethacrynic Acid / Q000032:analysis; D007546:Isoquinolines / Q000032:analysis; D046210:Microfluidic Analytical Techniques; D008853:Microscopy; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013059:Spectrum Analysis, Raman
chemical_list: D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D002244:Carbon; D003903:Deuterium; C039109:isoquinoline; D004976:Ethacrynic Acid