title: The isotype of autoantibodies influences the phagocytosis of antibody-coated platelets in autoimmune thrombocytopenic purpura.
abstract: Autoimmune thrombocytopenic purpura (AITP) is an acquired autoimmune bleeding disorder, characterized by isolated thrombocytopenia because of destruction of auto-antibody-coated platelets by Fc-receptor-mediated phagocytosis. The destruction of autoantibody-sensitized platelets by FcγR-bearing phagocytic cells and the following antigen presentation are considered to play a key role for the pathophysiology of AITP. Although different isotypes of AITP-mediating autoantibodies, e.g. IgG, IgM and IgA, are frequently found in AITP patients, their role in the pathophysiology of AITP remains unclear. Using a flow cytometric monocyte-based phagocytosis assay, we investigated the impact of disease-associated autoantibody isotype in antibody-mediated phagocytosis of platelets. Platelets, labelled with 5-chloromethyl fluorescein diacetate (CMFDA), were incubated with AITP patients' serum characterized by pure IgG or IgM antiplatelet autoantibodies. Labelled platelets were incubated with monocytes. Phagocytosis was defined as the product of percentage of CMFDA-positive monocytes and mean fluorescence intensity of CMFDA. Adherence of platelets to monocytes was quantified by anti-CD61-PerCp in a CMFDA(+)  CD14(+)  gate. IgG-coated platelets showed a significantly higher phagocytic index than IgM-coated platelets (mean 796 ± 157 versus 539 ± 78, P < 0.01). There were no significant differences regarding platelet adherence to monocytes. The isotype of autoantibodies influences the quantity of in vitro phagocytosis of autologous platelets by monocytes. Therefore, the AITP-mediating autoantibody isotype should be considered more carefully in pathophysiologic models and furthermore in diagnostic, therapeutic and prognostic approaches in AITP.
authors.affiliation: Institute of Transfusion Medicine and Transplantation Immunology, University Hospital Muenster, Germany. m.hoemberg@uni-muenster.de
authors.forename: M
authors.initials: M
authors.lastname: Hoemberg
authors.forename: D
authors.initials: D
authors.lastname: Stahl
authors.forename: P
authors.initials: P
authors.lastname: Schlenke
authors.forename: W
authors.initials: W
authors.lastname: Sibrowski
authors.forename: U
authors.initials: U
authors.lastname: Pachmann
authors.forename: U
authors.initials: U
authors.lastname: Cassens
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D007106:Immune Sera / Q000378:metabolism* / Q000494:pharmacology; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D007075:Immunoglobulin M / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D039661:Integrin beta3 / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D009000:Monocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010587:Phagocytosis / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D017452:Receptors, IgG / Q000276:immunology / Q000378:metabolism; D013921:Thrombocytopenia
chemical_list: D001323:Autoantibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D039661:Integrin beta3; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG