title: [Purification of geniposide in the extract fluid of Gardenia jasminoides with macroporous absorption resin].
abstract: OBJECTIVE
  To optimize the process for purification of geniposide in the extract fluid of Gardenia jasminoides with macroporous absorption resin.
  
  
  METHODS
  By comparing the content and transfer ratio of geniposide during the process of purification, we optimized the process for purification of geniposide.
  
  
  RESULTS
  The optimal process for purification of geniposide with D301R macroporous absorption resin included the diameter height ratio 1:7.5, the concentration of the extract fluid of Gardenia jasminoides 2:1, the flow rate 1BV/h (1BV represented one column volume), the sample volume 1/3BV. We loaded the sample and left it for 2 hours, afterwards, rinsed the macroporous absorption resin using 2BV water until the solution became colourless. Then we rinsed the macroporous absorption resin with 20% alcohol,and the volume of elution solvent was 2BV. We collected 20% alcohol elution solvent and recycle alcohol using rotating evaporation and dried the rest solution in a vacuum to get the light yellow powder which was the purified geniposide.
  
  
  CONCLUSIONS
  This process is simple and affordable, can be used to refine and purify geniposide in the extract fluid of Gardenia jasminoides Ellis. It provides a guidance for industrial production basis.
authors.affiliation: Shanghai University of TCM, Shanghai 201203, China. dingyue-2001@hotmail.com
authors.forename: Yue
authors.initials: Y
authors.lastname: Ding
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Tao
authors.forename: Yi-qiong
authors.initials: YQ
authors.lastname: Pu
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
mesh_terms: D000042:Absorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry*; D039823:Iridoids / Q000032:analysis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012117:Resins, Synthetic / Q000737:chemistry*; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D039823:Iridoids; D012117:Resins, Synthetic; D012997:Solvents; C007835:geniposide