title: Self-Assembly Pluronic and β-Cyclodextrin to Hollow Nanospheres for Enhanced Gene Delivery.
abstract: This paper studies a kind of hollow nanospheres prepared by self-assembly β-cyclodextrins (β-CDs) and poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (pluronic F127) for gene delivery. It was found that this kind of hollow nanospheres enable load PEI10K/DNA and the resulting F127NH2 βCD/(PEI10K/DNA) with 0.08 µg/well DNA display equal or higher gene delivery capability compared to PEI10K/DNA with 1 µg/well DNA in the absence or presence of serum. The cytotoxicity of the nanospheres was over 100 times lower than that of PEI10K.
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Min-Min
authors.initials: MM
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China. libj@cib.ac.cn.
authors.forename: Bang-Jing
authors.initials: BJ
authors.lastname: Li
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu 610041, China. xunsun22@gmail.com.
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China. zslbj@163.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D018014:Gene Transfer Techniques; D056945:Hep G2 Cells; D006801:Humans; D054118:Nanospheres / Q000737:chemistry*; D020442:Poloxamer / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D020442:Poloxamer