title: [Immunogenicity of influenza virus H5N2 vaccine strain samples produced by roller cultivation in media with plant derived components].
abstract: OBJECTIVE
  Study in CBA line mice of immunogenicity of cold adapted reassortant influenza virus H5N2 vaccine strain samples produced in rollers in MDCK and Vero cell cultures by using plant derived components.
  
  
  METHODS
  Antibody levels in blood sera and nasal swabs, lungs and small intestine of experimental vaccine strain sample immunized mice were evaluated by using HI reaction in accordance with WHO recommendations.
  
  
  RESULTS
  Reassortant vaccine strain A/17/duck/Potsdam/86/92 (H5N2) produced in MDCK and Vero cells by using plant derived components (rice and soy flour hydrolyzate and plant protease based nutrient medium) after intranasal immunization of mice induced local and humoral antibodies, and the latter not only against homologous virus, but also against highly pathogenic avian influenza virus strains A/ Chicken/Suzdalka/Nov-11/2005 and A/Chicken/Kurgan/05/2005.
  
  
  CONCLUSIONS
  Immunogenicity studies of reassortant influenza virus A/17/duck/Potsdam/86/92 (H5N2) vaccine strain samples cultivated in MDCK and Vero cells by using media with plant derived components in mice show high levels of humoral and secretory immunity.
authors.forename: N A
authors.initials: NA
authors.lastname: Mazurkova
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Desheva
authors.forename: L N
authors.initials: LN
authors.lastname: Shishkina
authors.forename: E A
authors.initials: EA
authors.lastname: Stavskiĭ
authors.forename: L G
authors.initials: LG
authors.lastname: Rudenko
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D019149:Bioreactors; D002522:Chlorocebus aethiops; D016895:Culture Media, Serum-Free / Q000737:chemistry; D004285:Dogs; D053125:Influenza A Virus, H5N2 Subtype / Q000254:growth & development* / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D012275:Oryza / Q000737:chemistry; D011492:Protein Hydrolysates / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry; D014709:Vero Cells; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral; D016895:Culture Media, Serum-Free; D007252:Influenza Vaccines; D011492:Protein Hydrolysates