title: Recycling and adaptation of Klebsormidium flaccidum microalgae for the sustained production of gold nanoparticles.
abstract: Targeting the development of cell-based bioreactors for the production of metal nanoparticles, the possibility to perform the sustained synthesis of colloidal gold using Klebsormidium flaccidum green algae was studied. A first strategy relying on successive growth/reduction/reseeding recycling steps demonstrated maintained biosynthesis capability of the microalgae but limitation in metal content due to toxic effects. An alternative approach consisting of progressive gold salt addition revealed to be suitable to favor cell adaptation to larger metal concentrations and supported particle release over month periods.
authors.affiliation: Univ Paris Diderot, Sorbonne Paris Cité, Interfaces, Traitements, Organisation et Dynamique des Systèmes, UMR 7086, CNRS, 15 rue Jean de Baïf, F-75205 Paris Cedex 13, France.
authors.forename: Si Amar
authors.initials: SA
authors.lastname: Dahoumane
authors.forename: Chakib
authors.initials: C
authors.lastname: Djediat
authors.forename: Claude
authors.initials: C
authors.lastname: Yéprémian
authors.forename: Alain
authors.initials: A
authors.lastname: Couté
authors.forename: Fernand
authors.initials: F
authors.lastname: Fiévet
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
authors.forename: Roberta
authors.initials: R
authors.lastname: Brayner
mesh_terms: D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D006046:Gold / Q000378:metabolism*; D053768:Metal Nanoparticles; D058086:Microalgae / Q000378:metabolism* / Q000502:physiology; D010788:Photosynthesis; D057949:Streptophyta / Q000378:metabolism* / Q000502:physiology
chemical_list: D006046:Gold