title: [Enterovirus 71 induces apoptosis in a Bax dependent manner].
abstract: OBJECTIVE
  To explore the molecular mechanism of apoptosis induced by enterovirus 71 (EV71) infection.
  
  
  METHODS
  The effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody.
  
  
  RESULTS
  EV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection.
  
  
  CONCLUSIONS
  Our study reveals that EV71 induces Caspase-dependent apoptosis by Bax conformational change.
authors.affiliation: State Key Laboratory of Molecular Virology and Genetic Engineering, Institute of Pathogen Biology, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, China
authors.forename: Zhen-min
authors.initials: ZM
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiao
authors.forename: Li-li
authors.initials: LL
authors.lastname: Ren
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Lei
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D020169:Caspases / Q000502:physiology; D045744:Cell Line, Tumor; D029821:Enterovirus A, Human / Q000472:pathogenicity*; D006801:Humans; D012208:Rhabdomyosarcoma / Q000473:pathology / Q000821:virology; D051028:bcl-2-Associated X Protein / Q000502:physiology*
chemical_list: C494826:BAX protein, human; D051028:bcl-2-Associated X Protein; D020169:Caspases