title: Diagnosis of gallbladder cancer using matrix-assisted laser desorption/ionization time-of-flight profiling.
abstract: Proteomic fingerprint technology combining magnetic beads with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to profile and compare the serum proteins from 45 patients with gallbladder cancer and 50 healthy blood donors. The proteomic patterns were identified; the tree model of biomarkers was constructed and evaluated using the Biomarker Patterns Software. The model tree was constructed based on the 3 biomarkers (5913 Da, 6181 Da and 13,752 Da), which generated excellent separation between the gallbladder cancer and control groups. The sensitivity was 86.7% and the specificity was 93.3%. The blind test data showed a sensitivity of 80% and a specificity of 90%. Taken together, our studies suggested that biomarkers for gallbladder cancer could be discovered in serum by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with the use of magnetic beads. The pattern of combined markers would provide a powerful and reliable diagnostic method for gallbladder cancer with high sensitivity and specificity.
authors.affiliation: Department of Surgery, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Mou
authors.affiliation: Department of Surgery, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Renwei
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang, China. Electronic address: liuchibo@126.com.
authors.forename: Chibo
authors.initials: C
authors.lastname: Liu
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry; D002277:Carcinoma / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D016022:Case-Control Studies; D002681:China; D003661:Decision Support Techniques; D003663:Decision Trees; D005260:Female; D005706:Gallbladder Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D059346:Magnets; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D010449:Peptide Mapping; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins