title: Trapping and imaging of micron-sized embryos using dielectrophoresis.
abstract: Development of dielectrophoretic (DEP) arrays for real-time imaging of embryonic organisms is described. Microelectrode arrays were used for trapping both embryonated eggs and larval stages of Antarctic nematode Panagrolaimus davidi. Ellipsoid single-shell model was also applied to study the interactions between DEP fields and developing multicellular organisms. This work provides proof-of-concept application of chip-based technologies for the analysis of individual embryos trapped under DEP force.
authors.affiliation: The BioMEMS Research Group, Department of Chemistry, University of Auckland, Auckland, New Zealand. khashayar.khoshmanesh@deakin.edu.au
authors.forename: Khashayar
authors.initials: K
authors.lastname: Khoshmanesh
authors.forename: Nimrod
authors.initials: N
authors.lastname: Kiss
authors.forename: Saeid
authors.initials: S
authors.lastname: Nahavandi
authors.forename: Clive W
authors.initials: CW
authors.lastname: Evans
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.forename: Donald
authors.initials: D
authors.lastname: Wlodkowic
mesh_terms: D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D007814:Larva / Q000737:chemistry / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D017168:Rhabditida / Q000196:embryology* / Q000302:isolation & purification