title: Genotyping of Japanese field isolates of Mycoplasma synoviae and rapid molecular differentiation from the MS-H vaccine strain.
abstract: Mycoplasma synoviae is an important causative agent of avian mycoplasmosis. In the present study the conserved domain of the variable lipoprotein and hemagglutinin (vlhA) gene of M. synoviae was sequenced and analyzed for 19 field strains of M. synoviae isolated from chickens across Japan. This analysis revealed that there were at least nine genotypes of M. synoviae present in Japan. Furthermore, we found a single nucleotide polymorphism (SNP) within this region in all the Japanese isolates, and based on this finding, we established a PCR method with cycling probe technology to differentiate between these field isolates and the live M. synoviae vaccine strain Mycoplasma synoviae-H (MS-H).
authors.affiliation: Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd., Asaka-machi, Koriyama-shi, Fukushima 963-0196, Japan. ogino-shoji@zenoaq.jp
authors.forename: Shoji
authors.initials: S
authors.lastname: Ogino
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Munakata
authors.forename: Shuichi
authors.initials: S
authors.lastname: Ohashi
authors.forename: Masato
authors.initials: M
authors.lastname: Fukui
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakamoto
authors.forename: Yukio
authors.initials: Y
authors.lastname: Sekiya
authors.forename: Amir H
authors.initials: AH
authors.lastname: Noormohammadi
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Morrow
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002645:Chickens; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D037102:Lectins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D045805:Mycoplasma synoviae / Q000235:genetics*; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D037102:Lectins; C406455:VlhA protein, Mycoplasma synoviae