title: In vitro variables of apheresis platelets are stably maintained for 7 days with 5% residual plasma in a glucose and bicarbonate salt solution, PAS-5.
abstract: BACKGROUND
  Platelet additive solutions (PASs) facilitate improved recovery of plasma and may reduce the severity and/or frequency of plasma-associated transfusion reactions. Current apheresis platelet (PLT) PAS products contain approximately 30 to 40% residual plasma. In an effort to further decrease the residual plasma, two in vitro studies were conducted with PLTs suspended in 5% plasma and a reformulated PAS-3, named PAS-5, that contains additional salts, glucose, and bicarbonate.
  
  
  METHODS
  In Study 1, PLTs suspended in 5% plasma/95% PAS-5 were prepared directly on a separator (Amicus, Fenwal, Inc.) without additional centrifugation or washing. In Study 2, a double unit of hyperconcentrated Amicus PLTs in plasma was collected, divided, and centrifuged to prepare a control unit in 100% plasma and a paired test unit in 5% plasma/95% PAS-5. The in vitro properties of PLTs were assessed in both studies during 7-day storage at 20 to 24°C with continuous agitation.
  
  
  RESULTS
  In Study 1, PLT concentration, pH, mean PLT volume (MPV), HCO(3)(-), pCO(2), pO(2), lactate dehydrogenase, and hypotonic shock response (HSR) did not significantly change during storage. By Day 7, glucose levels and morphology scores modestly decreased (17.6 and 14.4%, respectively) and lactate levels modestly increased (to 7.2 mmol/L). In Study 2, MPV, pH, glucose, pO(2), HSR, and morphology were comparable in control and test PLTs during 7-day storage. Glucose consumption and lactate production were significantly less in test versus control PLTs (p≤0.0015). Extent of shape change and %CD62P-positive test PLTs were less than those of controls (p<0.001).
  
  
  CONCLUSIONS
  Apheresis PLTs suspended in 5% plasma/95% PAS-5 maintained in vitro properties during 7-day storage.
authors.affiliation: Fenwal, Inc., Lake Zurich, Illinois 60047, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Radwanski
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wagner
authors.forename: Andrey
authors.initials: A
authors.lastname: Skripchenko
authors.forename: Kyungyoon
authors.initials: K
authors.lastname: Min
mesh_terms: D001639:Bicarbonates / Q000737:chemistry*; D001793:Blood Preservation / Q000379:methods*; D005947:Glucose / Q000737:chemistry*; D006801:Humans; D010949:Plasma / Q000737:chemistry*; D010983:Plateletpheresis; D012996:Solutions / Q000737:chemistry*
chemical_list: D001639:Bicarbonates; D012996:Solutions; D005947:Glucose