title: Plasminogen activator inhibitor-type I gene deficient mice show reduced influx of neutrophils in ventilator-induced lung injury.
abstract: Ventilator-induced lung injury (VILI) is associated with inhibition of the fibrinolytic system secondary to increased production of plasminogen activator inhibitor- (PAI-)1. To determine the role of PAI-1 on pulmonary coagulopathy and inflammation during mechanical ventilation, PAI-1 gene-deficient mice and their wild-type littermates were anesthetized (control), or anesthetized, tracheotomized and subsequently ventilated for 5 hours with either low tidal volumes (LV(T)) or high tidal volumes (HV(T)). VILI was assessed by pulmonary coagulopathy, lung wet-to-dry ratios, total protein level in bronchoalveolar lavage fluid, neutrophil influx, histopathology, and pulmonary and plasma cytokine levels. Ventilation resulted in pulmonary coagulopathy and inflammation, with more injury following ventilation with HV(T) as compared to LV(T). In PAI-1 gene-deficient mice, the influx of neutrophils in the pulmonary compartment was attenuated, while increased levels of pulmonary cytokines were found. Other endpoints of VILI were not different between PAI-1 gene-deficient and wild-type mice. These data indicate that a defect fibrinolytic response attenuates recruitment of neutrophils in VILI.
authors.affiliation: Department of Anesthesiology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
authors.forename: Esther K
authors.initials: EK
authors.lastname: Wolthuis
authors.forename: Alexander P J
authors.initials: AP
authors.lastname: Vlaar
authors.forename: Jorrit-Jan H
authors.initials: JJ
authors.lastname: Hofstra
authors.forename: Joris J T H
authors.initials: JJ
authors.lastname: Roelofs
authors.forename: Vivian
authors.initials: V
authors.lastname: de Waard
authors.forename: Nicole P
authors.initials: NP
authors.lastname: Juffermans
authors.forename: Marcus J
authors.initials: MJ
authors.lastname: Schultz