title: [Study of the interaction of 2'-5'-oligoadenylates and their analogues with proteins by fluorescence spectroscopy].
abstract: The interaction of "core" 2'-5'-oligoadenylates (2-5A) and their analogues with proteins albumin, interferon and immunoglobulin G was studied by fluorescence spectroscopy methods. Strong quenching of protein fluorescence (up to 67%) was observed upon interaction of oligoadenylates in concentration of 5 x 10(-5) M with albumin. Investigated compounds quenched the emission of interferon to a lesser extent, whereas no significant fluorescence changes occurred upon interaction with immunoglobulin under the same conditions. The level of quenching depended on the structure of 2-5A compounds and decreased with the decrease of their concentration. These data suggest that 2-5A actively bind to albumin and less efficiently to interferon, and practically do not interact with immunoglobulin. Taking into consideration different efficiency of quenching of the fluorescence excited at 280 and 296 nm, the assumption has been made about a possible role of tyrosine and tryptophan in the binding of a given compound to proteins. Possible mechanisms of interaction of oligoadenylates with proteins are discussed.
authors.forename: Z Iu
authors.initials: ZIu
authors.lastname: Tkachuk
authors.forename: L V
authors.initials: LV
authors.lastname: Dubeĭ
authors.forename: V V
authors.initials: VV
authors.lastname: Tkachuk
authors.forename: L V
authors.initials: LV
authors.lastname: Tkachuk
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Losyts'kyĭ
authors.forename: V M
authors.initials: VM
authors.lastname: Iashchuk
authors.forename: I Ia
authors.initials: IIa
authors.lastname: Dubeĭ
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D016898:Interferon-alpha / Q000737:chemistry*; D009842:Oligopeptides; D009843:Oligoribonucleotides / Q000737:chemistry*; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000227:Adenine Nucleotides; D007074:Immunoglobulin G; D016898:Interferon-alpha; D009842:Oligopeptides; D009843:Oligoribonucleotides; D012709:Serum Albumin; C048812:kinetensin; C023505:2',5'-oligoadenylate