title: Cloning, purification, crystallization and preliminary X-ray analysis of the catalytic domain of human receptor-like protein tyrosine phosphatase γ in three different crystal forms.
abstract: Protein tyrosine phosphatase γ is a membrane-bound receptor and is designated RPTPγ. RPTPγ and two mutants, RPTPγ(V948I, S970T) and RPTPγ(C858S, S970T), were recombinantly expressed and purified for X-ray crystallographic studies. The purified enzymes were crystallized using the hanging-drop vapor-diffusion method. Crystallographic data were obtained from several different crystal forms in the absence and the presence of inhibitor. In this paper, a description is given of how three different crystal forms were obtained that were used with various ligands. An orthorhombic crystal form and a trigonal crystal form were obtained both with and without ligand, and a monoclinic crystal form was only obtained in the presence of a particularly elaborated inhibitor.
authors.affiliation: Bristol-Myers Squibb Research and Development, Princeton, NJ 08543-4000, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Kish
authors.forename: Patricia A
authors.initials: PA
authors.lastname: McDonnell
authors.forename: Valentina
authors.initials: V
authors.lastname: Goldfarb
authors.forename: Mian
authors.initials: M
authors.lastname: Gao
authors.forename: William J
authors.initials: WJ
authors.lastname: Metzler
authors.forename: David R
authors.initials: DR
authors.lastname: Langley
authors.forename: James W
authors.initials: JW
authors.lastname: Bryson
authors.forename: Susan E
authors.initials: SE
authors.lastname: Kiefer
authors.forename: Brian
authors.initials: B
authors.lastname: Carpenter
authors.forename: Walter A
authors.initials: WA
authors.lastname: Kostich
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Westphal
authors.forename: Steven
authors.initials: S
authors.lastname: Sheriff
mesh_terms: D000595:Amino Acid Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D054557:Receptor-Like Protein Tyrosine Phosphatases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D054557:Receptor-Like Protein Tyrosine Phosphatases