title: The Mycobacterium tuberculosis CysQ phosphatase modulates the biosynthesis of sulfated glycolipids and bacterial growth.
abstract: CysQ is a 3'-phosphoadenosine-5'-phosphatase that dephosphorylates intermediates from the sulfate assimilation pathway of Mycobacterium tuberculosis (Mtb). Here, we demonstrate that cysQ disruption attenuates Mtb growth in vitro and decreases the biosynthesis of sulfated glycolipids but not major thiols, suggesting that the encoded enzyme specifically regulates mycobacterial sulfation.
authors.affiliation: Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
authors.forename: Stavroula K
authors.initials: SK
authors.lastname: Hatzios
authors.forename: Michael W
authors.initials: MW
authors.lastname: Schelle
authors.forename: Gerald L
authors.initials: GL
authors.lastname: Newton
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Sogi
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Holsclaw
authors.forename: Robert C
authors.initials: RC
authors.lastname: Fahey
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
mesh_terms: D002853:Chromatography, Liquid; D006017:Glycolipids / Q000096:biosynthesis* / Q000737:chemistry; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000254:growth & development; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D013431:Sulfates / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D013431:Sulfates; D010744:Phosphoric Monoester Hydrolases