title: Global phospholipidomics analysis reveals selective pulmonary peroxidation profiles upon inhalation of single-walled carbon nanotubes.
abstract: It is commonly believed that nanomaterials cause nonspecific oxidative damage. Our mass spectrometry-based oxidative lipidomics analysis of all major phospholipid classes revealed highly selective patterns of pulmonary peroxidation after inhalation exposure of mice to single-walled carbon nanotubes. No oxidized molecular species were found in the two most abundant phospholipid classes: phosphatidylcholine and phosphatidylethanolamine. Peroxidation products were identified in three relatively minor classes of anionic phospholipids, cardiolipin, phosphatidylserine, and phosphatidylinositol, whereby oxygenation of polyunsaturated fatty acid residues also showed unusual substrate specificity. This nonrandom peroxidation coincided with the accumulation of apoptotic cells in the lung. A similar selective phospholipid peroxidation profile was detected upon incubation of a mixture of total lung lipids with H(2)O(2)/cytochrome c known to catalyze cardiolipin and phosphatidylserine peroxidation in apoptotic cells. The characterized specific phospholipid peroxidation signaling pathways indicate new approaches to the development of mitochondria-targeted regulators of cardiolipin peroxidation to protect against deleterious effects of pro-apoptotic effects of single-walled carbon nanotubes in the lung.
authors.affiliation: Center for Free Radical and Antioxidant Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. yyt1@pitt.edu
authors.forename: Yulia Y
authors.initials: YY
authors.lastname: Tyurina
authors.forename: Elena R
authors.initials: ER
authors.lastname: Kisin
authors.forename: Ashley
authors.initials: A
authors.lastname: Murray
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Tyurin
authors.forename: Valentina I
authors.initials: VI
authors.lastname: Kapralova
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Sparvero
authors.forename: Andrew A
authors.initials: AA
authors.lastname: Amoscato
authors.forename: Alejandro K
authors.initials: AK
authors.lastname: Samhan-Arias
authors.forename: Linda
authors.initials: L
authors.lastname: Swedin
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
authors.forename: Bengt
authors.initials: B
authors.lastname: Fadeel
authors.forename: Anna A
authors.initials: AA
authors.lastname: Shvedova
authors.forename: Valerian E
authors.initials: VE
authors.lastname: Kagan
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D015227:Lipid Peroxidation; D008055:Lipids / Q000737:chemistry*; D008168:Lung / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D037742:Nanotubes, Carbon; D010750:Phosphoproteins / Q000737:chemistry*; D040901:Proteomics
chemical_list: D008055:Lipids; D037742:Nanotubes, Carbon; D010750:Phosphoproteins