title: Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC.
abstract: A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.
authors.affiliation: Department of Metabolic Care and Gerontology, University Hospital, Hradec Kralove, Czech Republic. Lenkakrcmova@seznam.cz
authors.forename: Lenka
authors.initials: L
authors.lastname: Krcmova
authors.forename: Dagmar
authors.initials: D
authors.lastname: Solichova
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Melichar
authors.forename: Marketa
authors.initials: M
authors.lastname: Kasparova
authors.forename: Jiri
authors.initials: J
authors.lastname: Plisek
authors.forename: Luboš
authors.initials: L
authors.lastname: Sobotka
authors.forename: Petr
authors.initials: P
authors.lastname: Solich
mesh_terms: D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003404:Creatinine / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007737:Kynurenine / Q000097:blood*; D019798:Neopterin / Q000097:blood*; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D014364:Tryptophan / Q000097:blood*
chemical_list: D002021:Buffers; D010710:Phosphates; D007737:Kynurenine; D019798:Neopterin; D014364:Tryptophan; D003404:Creatinine