title: Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection.
abstract: OBJECTIVE
  Respiratory syncytial virus (RSV) is the most common cause of severe respiratory tract infection in infants. The aim was to identify host defence components in nasopharyngeal aspirate (NPA) from infants with RSV infection and to study the expression of the novel 25 kDa innate immunity protein SPLUNC1.
  
  
  METHODS
  NPAs from infants were analyzed with 2-DE and MS in a pilot study. The levels of SPLUNC1 were analyzed with immunoblotting in 47 NPAs, admitted for RSV diagnosis.
  
  
  RESULTS
  Totally, 35 proteins were identified in NPA, including several innate immunity proteins such as group X phospholipase A(2) , different S100 proteins and SPLUNC1. In addition, a new truncated 15 kDa form of SPLUNC1 was identified that was detected in about 50% of the aspirates admitted for RSV diagnosis. RSV-positive boys had significantly less 25 kDa SPLUNC1 than RSV-negative boys while there were no significant differences among girls.
  
  
  CONCLUSIONS
  Several important innate immunity proteins were identified in NPA. Notably, a new truncated form of the newly suggested anti-bacterial protein SPLUNC1 was found. It is possible that a decrease in SPLUNC1 in the upper airways may increase the risk for severe pneumonia in boys.
authors.affiliation: Occupational and Environmental Medicine, Department of Clinical and Experimental Medicine, Linköping University, Sweden.
authors.forename: Louise
authors.initials: L
authors.lastname: Fornander
authors.forename: Bijar
authors.initials: B
authors.lastname: Ghafouri
authors.forename: Erik
authors.initials: E
authors.lastname: Kihlström
authors.forename: Britt
authors.initials: B
authors.lastname: Akerlind
authors.forename: Thomas
authors.initials: T
authors.lastname: Schön
authors.forename: Christer
authors.initials: C
authors.lastname: Tagesson
authors.forename: Mats
authors.initials: M
authors.lastname: Lindahl
mesh_terms: D002675:Child, Preschool; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D054509:Group X Phospholipases A2 / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D015151:Immunoblotting / Q000379:methods; D007223:Infant; D008297:Male; D009305:Nasopharynx / Q000378:metabolism* / Q000821:virology; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism*; D010865:Pilot Projects; D020033:Protein Isoforms / Q000276:immunology / Q000378:metabolism; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis*; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification; D009418:S100 Proteins / Q000378:metabolism; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C485599:BPIFA1 protein, human; D006023:Glycoproteins; D010750:Phosphoproteins; D020033:Protein Isoforms; D009418:S100 Proteins; D054509:Group X Phospholipases A2