title: Mutation analysis of the tyrosine phosphatase PTPN2 in Hodgkin's lymphoma and T-cell non-Hodgkin's lymphoma.
abstract: We recently reported deletion of the protein tyrosine phosphatase gene PTPN2 in T-cell acute lymphoblastic leukemia. Functional analyses confirmed that PTPN2 acts as classical tumor suppressor repressing the proliferation of T cells, in part through inhibition of JAK/STAT signaling. We investigated the expression of PTPN2 in leukemia as well as lymphoma cell lines. We identified bi-allelic inactivation of PTPN2 in the Hodgkin's lymphoma cell line SUP-HD1 which was associated with activation of the JAK/STAT pathway. Subsequent sequence analysis of Hodgkin's lymphoma and T-cell non-Hodgkin's lymphoma identified bi-allelic inactivation of PTPN2 in 2 out of 39 cases of peripheral T-cell lymphoma not otherwise specified, but not in Hodgkin's lymphoma. These results, together with our own data on T-cell acute lymphoblastic leukemia, demonstrate that PTPN2 is a tumor suppressor gene in T-cell malignancies.
authors.affiliation: Department of Molecular and Developmental Genetics, VIB, Leuven, Belgium.
authors.forename: Maria
authors.initials: M
authors.lastname: Kleppe
authors.forename: Thomas
authors.initials: T
authors.lastname: Tousseyn
authors.forename: Eva
authors.initials: E
authors.lastname: Geissinger
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Kalender Atak
authors.forename: Stein
authors.initials: S
authors.lastname: Aerts
authors.forename: Andreas
authors.initials: A
authors.lastname: Rosenwald
authors.forename: Iwona
authors.initials: I
authors.lastname: Wlodarska
authors.forename: Jan
authors.initials: J
authors.lastname: Cools
mesh_terms: D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis; D005260:Female; D006689:Hodgkin Disease / Q000201:enzymology / Q000235:genetics; D006801:Humans; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism; D008297:Male; D009154:Mutation; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000201:enzymology / Q000235:genetics; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2 / Q000235:genetics / Q000378:metabolism; D050791:STAT Transcription Factors / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D050791:STAT Transcription Factors; D025521:Tumor Suppressor Proteins; D053612:Janus Kinases; C516649:PTPN2 protein, human; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2