title: Acaricidal activity of thymol on larvae and nymphs of Amblyomma cajennense (Acari: Ixodidae).
abstract: The aim of the present study was to evaluate the acaricidal efficacy of thymol on unengorged and engorged larvae and engorged nymphs of Amblyomma cajennense. To perform the test for unengorged larvae, the larval packet technique was employed and mortality was evaluated 24h after the test, while for engorged larvae and engorged nymphs the immersion technique was employed and mortality was evaluated after 15 days. In all the experiments, the following concentrations of thymol were tested: 2.5, 5.0, 10.0, 15.0 and 20.0mg the thymol/ml. The control group was exposed to water and 1% DMSO and there were 10 repetitions for each treatment. The values found for mortality of unengorged larvae were 18.2%, 51.8%, 97.6%, 93.5% and 94.5%, and for engorged nymphs the values were 26.0%, 92.2%, 100.0%, 100.0%, 100.0%, in concentrations of 2.5, 5.0, 10.0, 15.0 and 20.0mg of thymol/ml, respectively. In the test with engorged larvae, mortality was 100.0% in all treatments. Based on the results, it is possible to conclude that thymol has acaricidal activity against immature stages of A. cajennense.
authors.affiliation: Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário Bairro Martelos, 36036-330 Juiz de Fora, MG, Brazil.
authors.forename: Andressa da Silva
authors.initials: Ada S
authors.lastname: Mendes
authors.forename: Erik
authors.initials: E
authors.lastname: Daemon
authors.forename: Caio Márcio de Oliveira
authors.initials: CM
authors.lastname: Monteiro
authors.forename: Ralph
authors.initials: R
authors.lastname: Maturano
authors.forename: Fernanda Calmon
authors.initials: FC
authors.lastname: Brito
authors.forename: Tainara
authors.initials: T
authors.lastname: Massoni
mesh_terms: D056810:Acaricides; D000818:Animals; D005260:Female; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses; D026863:Ixodidae; D007814:Larva; D009758:Nymph; D016019:Survival Analysis; D013943:Thymol; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary*; D013997:Time Factors
chemical_list: D056810:Acaricides; D013943:Thymol