title: Increased iron supplied through Fet3p results in replicative life span extension of Saccharomyces cerevisiae under conditions requiring respiratory metabolism.
abstract: We have previously shown that copper supplementation extends the replicative life span of Saccharomyces cerevisiae when grown under conditions forcing cells to respire. We now show that copper's effect on life span is through Fet3p, a copper containing enzyme responsible for high affinity transport of iron into yeast cells. Life span extensions can also be obtained by supplementing the growth medium with 1mM ferric chloride. Extension by high iron levels is still dependent on the presence of Fet3p. Life span extension by iron or copper requires growth on media containing glycerol as the sole carbon source, which forces yeast to respire. Yeast grown on glucose containing media supplemented with iron show no extension of life span. The iron associated with cells grown in media supplemented with copper or iron is 1.4-1.8 times that of cells grown without copper or iron supplementation. As with copper supplementation, iron supplementation partially rescues the life span of superoxide dismutase mutants. Cells grown with copper supplementation display decreased production of superoxide as measured by dihydroethidium staining.
authors.affiliation: Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431, United States. Gabriela.Botta@biomet.com
authors.forename: Gabriela
authors.initials: G
authors.lastname: Botta
authors.forename: Christina S
authors.initials: CS
authors.lastname: Turn
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Quintyne
authors.forename: Paul A
authors.initials: PA
authors.lastname: Kirchman
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000235:genetics; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D019069:Cell Respiration / Q000235:genetics* / Q000502:physiology; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005990:Glycerol / Q000494:pharmacology; D007501:Iron / Q000378:metabolism / Q000494:pharmacology*; D008017:Life Expectancy; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D027682:Cation Transport Proteins; D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D003300:Copper; D007501:Iron; D005990:Glycerol