title: Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy.
abstract: Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
authors.affiliation: Program in Molecular Pharmacology and Chemistry, Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
authors.forename: Tony
authors.initials: T
authors.lastname: Taldone
authors.forename: Erica M
authors.initials: EM
authors.lastname: Gomes-DaGama
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zong
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Sen
authors.forename: Mary L
authors.initials: ML
authors.lastname: Alpaugh
authors.forename: Danuta
authors.initials: D
authors.lastname: Zatorska
authors.forename: Raul
authors.initials: R
authors.lastname: Alonso-Sabadell
authors.forename: Monica L
authors.initials: ML
authors.lastname: Guzman
authors.forename: Gabriela
authors.initials: G
authors.lastname: Chiosis
mesh_terms: D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry*; D018841:HSP90 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011687:Purines / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins; D011687:Purines; C030985:purine