title: Optimization of monosaccharide determination using anthranilic acid and 1-phenyl-3-methyl-5-pyrazolone for gastropod analysis.
abstract: The protein-linked glycomes and, thereby, the range of individual monosaccharides of invertebrates differ from those of mammals due to a number of special modifications; therefore, it is necessary to adapt methods for monosaccharide analysis in order to cover these. We optimized the labeling procedure for anthranilic acid (AA) and 1-phenyl-3-methyl-5-pyrazolone (PMP) and the subsequent separation of the labeled monosaccharides on high-performance liquid chromatography (HPLC), with the result that we were able to identify 26 different monosaccharides. The detection limit for anthranilic acid derivatives obtained was 65 fmol, and a reliable quantification of samples was possible up to 200 nmol under the tested conditions. PMP derivatives showed a significantly higher detection limit but allow quantification of larger sample amounts. Applying these methods on snails, their impressive set of monosaccharide constituents, including methylated sugars, was shown.
authors.affiliation: Department of Chemistry, University of Natural Resources and Life Sciences, A-1190 Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Stepan
authors.forename: E
authors.initials: E
authors.lastname: Staudacher
mesh_terms: D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D000077553:Edaravone; D049851:Gastropoda / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents; D009005:Monosaccharides / Q000032:analysis*; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D009005:Monosaccharides; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D000077553:Edaravone; D000983:Antipyrine