title: Effects of L-arginine and NG-nitro L-arginine methyl ester (L-NAME) on ischemia/reperfusion injury of skeletal muscle, small and large intestines.
abstract: This study analyzed the effects of L-arginine and non-specific nitric oxide (NO) synthase blocker (L-NAME) on structural and metabolic changes in experimental ischemia/reperfusion injury in the rat. Histopathological evaluation of rat tissues after reperfusion was also performed. The animals were divided into four groups: [1] nonischemic control, [2] ischemia 4 hrs/repefusion 30, 60, 120 min, [3] ischemia/reperfusion after L-arginine administration, [4] ischemia/reperfusion, after L-arginine, and L-NAME. L-arginine (500 mg/kg) and L-NAME (75 micromol/rat/day) were administrated orally for 5 days before experiment. Concentrations of free radicals, CD-62P, CD-54 and malonyl dialdehyde (MDA) in tissues, and MDA and NO levels in sera were determined. Free radical levels significantly increased in reperfused skeletal muscle, small and large intestines. In large bowel, reperfusion increased MDA levels and evoked a rise of endotoxin level while NO levels decreased. Histological studies showed an increase in the number of lymphocytes in both intestines. Administration of L-arginine reduced leukocyte adherence associated with ischemia-repefusion injury, decreased the levels of free radicals and MDA in the examined tissues, and inhibited the release of endotoxins into blood. L-arginine-treated animals showed higher serum NO levels and reduced leukocyte bowel infiltration. Concomitant L-NAME administration reduced serum NO and tissue free radical [corrected] levels, but did not affect intestinal leukocyte infiltration. L-arginine could ameliorate intestinal ischemia/reperfusion injury and constitute a possible protective mechanism by decreasing neutrophil-endothelial interactions, stimulating free radical scavenging and reducing lipid peroxidation.
authors.affiliation: Department of Physiology, K. Marcinkowski University of Medical Sciences, Poznan, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Krauss
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Sosnowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Biczysko
authors.forename: Wieslawa
authors.initials: W
authors.lastname: Biczysko
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Majewski
authors.forename: Anna
authors.initials: A
authors.lastname: Jablecka
authors.forename: Bogdan
authors.initials: B
authors.lastname: Miskowiak
authors.forename: Iwona
authors.initials: I
authors.lastname: Smolarek
authors.forename: Aneta
authors.initials: A
authors.lastname: Konwerska
authors.forename: Iwona
authors.initials: I
authors.lastname: Ignys
authors.forename: Maciej
authors.initials: M
authors.lastname: Micker
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D004791:Enzyme Inhibitors; D005609:Free Radicals; D009569:Nitric Oxide; D008315:Malondialdehyde; D001120:Arginine; D019331:NG-Nitroarginine Methyl Ester