title: [Chymotrypsin and trypsin inhibitor isolated from potato tubers].
abstract: Potato Kunitz-type chymotrypsin inhibitor (PKCI-23) was isolated from potato tubers (Solanum tuberosum L., Zhukov's Jubilee breed) and purified to a homogenous state. The protein was purified by gel-filtration chromatography and ion-exchange chromatography using Sephadex G-75 and CM-Sepharose CL-6B, respectively. PKCI-23 protein has been shown to inhibit both chymotrypsin and trypsin with equal efficacy, forming equimolar complexes with these enzymes. However, much weaker inhibitory effect of PKCI-23 has been observed for Carlsberg subtilisin. The N-terminal 20 amino acid sequence of PKCI-23 has been sequenced. PKCI-23 has been shown to suppress, with different efficacy, the growth and development of pathogenic microorganisms Fusarium culmorum (Wm. G. Sm.) Sacc. and Phytophtora infestans (Mont.) de Bary that infect potato.
authors.forename: T A
authors.initials: TA
authors.lastname: Revina
authors.forename: I A
authors.initials: IA
authors.lastname: Parfenov
authors.forename: E L
authors.initials: EL
authors.lastname: Gvozdeva
authors.forename: N G
authors.initials: NG
authors.lastname: Gerasimova
authors.forename: T A
authors.initials: TA
authors.lastname: Valueva
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D055750:Phytophthora infestans / Q000187:drug effects / Q000254:growth & development; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D035281:Plant Tubers / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry; D020860:Subtilisin / Q000037:antagonists & inhibitors; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D010940:Plant Proteins; D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D014357:Trypsin; D020860:Subtilisin