title: Is cell death induced by nematocysts extract of medusa Pelagia noctiluca related to oxidative stress?
abstract: Pelagia noctiluca, a jellyfish widely distributed in the Mediterranean waters, especially in coastal areas of Tunisia, has garnered attention because of its stinging capacity and the resulting public health hazard. Crude extracts of P. noctiluca nematocysts have been tested for their cytotoxicity on Vero cells. Our results clearly showed that nematocysts induced cell mortality in a dose- and time-dependent manner. A cytoprotective effect against cell mortality was obtained when Vero cells were treated with Vitamin E. This process was further confirmed by the generation of reactive oxygen species (ROS) and the induction of Hsp 70 and 27 protein expressions. Thus, our findings suggested that oxidative stress is involved in the toxicity of pelagia nematocysts and may therefore constitute the major mechanism of this medusa nematocysts toxicity.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia.
authors.forename: Yosra
authors.initials: Y
authors.lastname: Ayed
authors.forename: Bouaziz
authors.initials: B
authors.lastname: Chayma
authors.forename: Abassi
authors.initials: A
authors.lastname: Hayla
authors.forename: Salwa
authors.initials: S
authors.lastname: Abid
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D003064:Cnidarian Venoms / Q000633:toxicity*; D019610:Cytoprotection; D003603:Cytotoxins / Q000633:toxicity*; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D058894:Nematocyst / Q000737:chemistry*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D037401:Scyphozoa / Q000737:chemistry*; D014020:Tissue Extracts / Q000633:toxicity*; D014709:Vero Cells; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D003064:Cnidarian Venoms; D003603:Cytotoxins; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D017382:Reactive Oxygen Species; D014020:Tissue Extracts; D014810:Vitamin E