title: Bacillus amyloliquefaciens: a mosquitocidal bacterium from mangrove forests of Andaman & Nicobar islands, India.
abstract: Samples collected from the mangrove forests of Andaman & Nicobar islands yielded a mosquitocidal bacterium, whose extracellular metabolite(s) exhibited mosquito larvicidal and pupicidal activity. The bacterium was isolated using standard microbiological methods and identified using classical biochemical tests and rpoB gene sequences. The mosquitocidal bacterium was identified as Bacillus amyloliquefaciens. Mosquitocidal metabolite(s) was separated from the culture supernatant of the bacterium and its efficacy against the larval and pupal stages of different species of mosquitoes was determined in terms of LC(50) and LC(90). Mosquito larvicidal activity in terms of LC(50) against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti was respectively, 26.4μg, 22.2μg and 20.5μg/ml and its pupicidal activity was 4.4μg, 8.2μg and 14.5μg/ml respectively. The mosquitocidal metabolite(s) was found to be a biosurfactant. This is the first report of the mosquitocidal activity of B. amyloliquefaciens and it is a new weapon which can be added to the array of microbial agents for use against mosquitoes.
authors.affiliation: Unit of Microbiology & Immunology, Vector Control Research Centre, Indian Council of Medical Research (ICMR), Indira Nagar, Puducherry, India.
authors.forename: I
authors.initials: I
authors.lastname: Geetha
authors.forename: A M
authors.initials: AM
authors.lastname: Manonmani
authors.forename: G
authors.initials: G
authors.lastname: Prabakaran
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000254:growth & development; D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000254:growth & development; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D003465:Culex / Q000187:drug effects* / Q000254:growth & development; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004783:Environmental Microbiology; D007194:India; D007306:Insecticides / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011679:Pupa / Q000187:drug effects / Q000254:growth & development; D017422:Sequence Analysis, DNA; D016019:Survival Analysis; D014197:Trees
chemical_list: D004269:DNA, Bacterial; D007306:Insecticides; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit