title: Hybrid thermochemical processing: fermentation of pyrolysis-derived bio-oil.
abstract: Thermochemical processing of biomass by fast pyrolysis provides a nonenzymatic route for depolymerization of biomass into sugars that can be used for the biological production of fuels and chemicals. Fermentative utilization of this bio-oil faces two formidable challenges. First is the fact that most bio-oil-associated sugars are present in the anhydrous form. Metabolic engineering has enabled utilization of the main anhydrosugar, levoglucosan, in workhorse biocatalysts. The second challenge is the fact that bio-oil is rich in microbial inhibitors. Collection of bio-oil in distinct fractions, detoxification of bio-oil prior to fermentation, and increased robustness of the biocatalyst have all proven effective methods for addressing this inhibition.
authors.affiliation: Chemical and Biological Engineering, Iowa State University, Ames, IA, USA. LJarboe@iastate.edu
authors.forename: Laura R
authors.initials: LR
authors.lastname: Jarboe
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Wen
authors.forename: DongWon
authors.initials: D
authors.lastname: Choi
authors.forename: Robert C
authors.initials: RC
authors.lastname: Brown
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D056804:Biofuels / Q000032:analysis / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry*; D005285:Fermentation; D005658:Fungi / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006868:Hydrolysis
chemical_list: D056804:Biofuels; D002241:Carbohydrates