title: Association of two porcine reproductive and respiratory syndrome virus (PRRSV) receptor genes, CD163 and SN with immune traits.
abstract: CD163 and sialoadhesin (SN) were reported as two essential receptors for the porcine reproductive and respiratory syndrome virus. To investigate the relationship between these two genes and porcine immunity, we assigned porcine CD163 and SN respectively to SSC5q21-q24 and SSC17q23 by IMpRH. Expression profiles revealed that CD163 and SN were ubiquitously expressed in ten tissues, and were expressed highly in lymph gland, spleen and liver, which implied the potential functions of CD163 and SN in immunity. Moreover, a single nucleotide polymorphism (SNP) c.3534C>T was found in 3'-UTR of the CD163 gene and association analysis showed that this gene was significantly associated with the IgG content in blood (P < 0.05). A novel missense mutation c.878A>G located in exon4 of the SN gene which caused the amino acid transition from histidine to arginine was detected, and it was significantly associated with the WBC count in the peripheral blood (P < 0.05). These results provided fundamental evidence for CD163 and SN as two functional candidate genes affecting immunity in pigs.
authors.affiliation: Laboratory of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Fengli
authors.initials: F
authors.lastname: Wang
authors.forename: Haifang
authors.initials: H
authors.lastname: Qiu
authors.forename: Qingde
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhongzhen
authors.initials: Z
authors.lastname: Peng
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D033481:Chromosomes, Mammalian / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D005838:Genotype; D008562:Membrane Glycoproteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019316:Porcine respiratory and reproductive syndrome virus / Q000378:metabolism*; D019655:Quantitative Trait, Heritable; D011956:Receptors, Cell Surface / Q000235:genetics*; D011971:Receptors, Immunologic / Q000235:genetics*; D011991:Receptors, Virus / Q000235:genetics*; D063267:Sialic Acid Binding Ig-like Lectin 1; D034421:Sus scrofa / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011991:Receptors, Virus; D063267:Sialic Acid Binding Ig-like Lectin 1