title: Extracellular ATP induces CD44 shedding from macrophage-like P388D1 cells via the P2X7 receptor.
abstract: The P2X7 receptor (P2X7R) is a nucleotide receptor expressed predominantly on hemopoietic, bone, and epithelial cells. The P2X7R can be activated by extracellular ATP and induces the influx of calcium, releases cytokines, and participates in cell proliferation and apoptosis. CD44 is an adhesion molecule. The effects of CD44 include cell-cell and cell-matrix adhesion interactions, lymphocyte activation, and cell migration. Many studies have shown that P2X7R and CD44 play important roles in hematological malignancies, but no study exists regarding the relationship between P2X7R and CD44. In the present study, we characterized P388D1 cells for the surface expression of CD44 and analyzed ATP-induced shedding. The data showed that P388D1 cells express CD44. Incubation of P388D1 cells with ATP induced a rapid loss of CD44 from the P388D1 cell surface. In addition, using a receptor inhibitor and P2X7R short hairpin RNA, we showed that the loss of CD44 is mediated via the P2X7R. Finally, we demonstrated that activation of P2X7R by ATP induces CD44 shedding.
authors.affiliation: Department of General Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Lin
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Ren
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hongfei
authors.initials: H
authors.lastname: Jin
authors.forename: Jianguo
authors.initials: J
authors.lastname: Sun
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000818:Animals; D045744:Cell Line, Tumor; D005110:Extracellular Space / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000032:analysis / Q000502:physiology*; D007941:Leukemia P388 / Q000378:metabolism*; D051379:Mice; D058486:Receptors, Purinergic P2X7 / Q000502:physiology*
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate