title: Clostridium beijerinckii mutant with high inhibitor tolerance obtained by low-energy ion implantation.
abstract: Clostridium beijerinckii mutant strain IB4, which has a high level of inhibitor tolerance, was screened by low-energy ion implantation and used for butanol fermentation from a non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). Evaluation of toxicity showed C. beijerinckii IB4 had a higher level of tolerance than parent strain C. beijerinckii NCIMB 8052 for five out of six phenolic compounds tested (the exception was vanillin). Using glucose as carbon source, C. beijerinckii IB4 produced 9.1 g l(-1) of butanol with an acetone/butanol/ethanol (ABE) yield of 0.41 g g(-1). When non-detoxified SAHHC was used as carbon source, C. beijerinckii NCIMB 8052 grew well but ABE production was inhibited. By contrast, C. beijerinckii IB4 produced 9.5 g l(-1) of ABE with a yield of 0.34 g g(-1), including 2.2 g l(-1) acetone, 6.8 g l(-1) butanol, and 0.5 g l(-1) ethanol. The remarkable fermentation and inhibitor tolerance of C. beijerinckii IB4 appears promising for ABE production from lignocellulosic materials.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.forename: Qiu-Yan
authors.initials: QY
authors.lastname: Zhang
authors.forename: Teng-Fei
authors.initials: TF
authors.lastname: Du
authors.forename: Da-Feng
authors.initials: DF
authors.lastname: Liang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping-Kai
authors.initials: PK
authors.lastname: Ouyang
mesh_terms: D000096:Acetone / Q000378:metabolism; D000818:Animals; D001673:Biodegradation, Environmental; D000440:Butanols / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000235:genetics* / Q000502:physiology; D004361:Drug Tolerance / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009154:Mutation; D011492:Protein Hydrolysates / Q000378:metabolism; D003313:Zea mays
chemical_list: D000440:Butanols; D011492:Protein Hydrolysates; D000096:Acetone; D000431:Ethanol; D002482:Cellulose; D005947:Glucose