title: The influence of metabolic gene polymorphisms on urinary 1-hydroxypyrene concentration in Thai bus drivers.
abstract: Polycyclic aromatic hydrocarbons (PAHs) are associated with an increased cancer risk. CYP1A1 and GSTs enzymes are important in metabolism of PAHs. Genetic polymorphisms of these enzymes are responsible for enzyme activity and concentration variation. The objectives of this study were to evaluate association of 1-OHP concentration with genetic polymorphisms of CYP1A1 and GSTs in Thai bus drivers. The results showed that 1-OHP levels in bus drivers were significantly higher than that in the control group. Significant difference in 1-OHP was found between smokers and non-smokers, in only bus drivers. Significantly increasing of 1-OHP levels were observed in bus drivers with CYP1A1 MspI and exon 7 variants. Whereas, bus drivers with GSTP1 Val and GSTM1 null genotypes showed decreasing in excretion of 1-OHP. No association between 1-OHP and polymorphisms of GSTT1 was found. This study indicated that 1-OHP concentrations were associated with exposure to air pollution, cigarette smoking and polymorphisms of CYP1A1, GSTM1 and GSTP1 genes.
authors.affiliation: Toxicology Graduate Programme, Faculty of Science, Mahidol University, Thailand.
authors.forename: Krittaya
authors.initials: K
authors.lastname: Petchpoung
authors.forename: Sming
authors.initials: S
authors.lastname: Kaojarern
authors.forename: Krongtong
authors.initials: K
authors.lastname: Yoovathaworn
authors.forename: Thanyachai
authors.initials: T
authors.lastname: Sura
authors.forename: Jintana
authors.initials: J
authors.lastname: Sirivarasai
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000235:genetics / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004247:DNA / Q000235:genetics; D005091:Exons / Q000235:genetics; D005260:Female; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D018986:Motor Vehicles; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D011721:Pyrenes / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects; D013785:Thailand
chemical_list: D019363:Cytochrome P-450 CYP1A1; D004247:DNA; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C413545:glutathione S-transferase T1; C496557:Gstp1 protein, mouse; C033146:1-hydroxypyrene