title: Pmarg-pearlin is a matrix protein involved in nacre framework formation in the pearl oyster Pinctada margaritifera.
abstract: The shell of pearl oysters is organized in multiple layers of CaCO(3)  crystallites packed together in an organic matrix. Relationships between the components of the organic matrix and mechanisms of nacre formation currently constitute the main focus of research into biomineralization. In this study, we characterized the pearlin protein from the oyster Pinctada margaritifera (Pmarg); this shares structural features with other members of a matrix protein family, N14/N16/pearlin. Pmarg pearlin exhibits calcium- and chitin-binding properties. Pmarg pearlin transcripts are distinctively localized in the mineralizing tissue responsible for nacre formation. More specifically, we demonstrate that Pmarg pearlin is localized within the interlamellar matrix of nacre aragonite tablets. Our results support recent models for multidomain matrix protein involvement in nacreous layer formation. We provide evidence here for the existence of a conserved family of nacre-associated proteins in Pteriidae, and reassess the evolutionarily conserved set of biomineralization genes related to nacre formation in this taxa.
authors.affiliation: Laboratoire Biotechnologie et Qualité de la Perle, Ifremer, Centre Océanologique du Pacifique, BP 7004, 98719 Taravao, Tahiti, French Polynesia. cmontagn@ifremer.fr
authors.forename: C
authors.initials: C
authors.lastname: Montagnani
authors.forename: B
authors.initials: B
authors.lastname: Marie
authors.forename: F
authors.initials: F
authors.lastname: Marin
authors.forename: C
authors.initials: C
authors.lastname: Belliard
authors.forename: F
authors.initials: F
authors.lastname: Riquet
authors.forename: A
authors.initials: A
authors.lastname: Tayalé
authors.forename: I
authors.initials: I
authors.lastname: Zanella-Cléon
authors.forename: E
authors.initials: E
authors.lastname: Fleury
authors.forename: Y
authors.initials: Y
authors.lastname: Gueguen
authors.forename: D
authors.initials: D
authors.lastname: Piquemal
authors.forename: N
authors.initials: N
authors.lastname: Cochennec-Laureau
mesh_terms: D000595:Amino Acid Sequence; D060105:Animal Shells / Q000378:metabolism; D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D049911:Pinctada / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D012333:RNA, Messenger