title: Noninvasive, transient and selective blood-brain barrier opening in non-human primates in vivo.
abstract: The blood-brain barrier (BBB) is a specialized vascular system that impedes entry of all large and the vast majority of small molecules including the most potent central nervous system (CNS) disease therapeutic agents from entering from the lumen into the brain parenchyma. Microbubble-enhanced, focused ultrasound (ME-FUS) has been previously shown to disrupt noninvasively, selectively, and transiently the BBB in small animals in vivo. For the first time, the feasibility of transcranial ME-FUS BBB opening in non-human primates is demonstrated with subsequent BBB recovery. Sonications were combined with two different types of microbubbles (customized 4-5 µm and Definity®). 3T MRI was used to confirm the BBB disruption and to assess brain damage.
authors.affiliation: Department of Biomedical Engineering, Columbia University, New York, New York, United States of America.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Marquet
authors.forename: Yao-Sheng
authors.initials: YS
authors.lastname: Tung
authors.forename: Tobias
authors.initials: T
authors.lastname: Teichert
authors.forename: Vincent P
authors.initials: VP
authors.lastname: Ferrera
authors.forename: Elisa E
authors.initials: EE
authors.lastname: Konofagou
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging* / Q000293:injuries / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D008279:Magnetic Resonance Imaging; D045423:Microbubbles; D011323:Primates; D013997:Time Factors; D014465:Ultrasonics / Q000295:instrumentation / Q000379:methods*; D014463:Ultrasonography