title: Up-regulation of hypoxia-inducible factor-1 alpha by cobalt chloride prevents hearing loss in noise-exposed mice.
abstract: Since hypoxia-inducible factor-1α (HIF-1α) is the key transcription factor that enables cells to survive in hypoxia, we have investigated whether an upregulation of HIF-1α prevents the noise-induced hearing loss in BALB/c hybrid mice, which were intraperitoneally injected with CoCl(2) (a HIF-1α inducer) and exposed to white band noise with 120 dB peak equivalent sound pressure level for 3h once daily for 3 days. In the CoCl(2) treatment group, HIF-1α was found to be up-regulated in the cochlear tissues and the hearing loss was largely prevented. Histologically, the loss of sensory hair cells was also significantly lower in the CoCl(2) treatment group than the Control group. However, YC-1 (a HIF-1α inhibitor) attenuated the preventive effect of CoCl(2) on the noise-induced hearing loss. These results suggest that HIF-1α plays a crucial role in the prevention against noise trauma in the inner ear.
authors.affiliation: Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea. jwchung@amc.seoul.kr
authors.forename: Jong Woo
authors.initials: JW
authors.lastname: Chung
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Shin
authors.forename: Kwang Woo
authors.initials: KW
authors.lastname: Han
authors.forename: Joong Ho
authors.initials: JH
authors.lastname: Ahn
authors.forename: Young-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jong-Wan
authors.initials: JW
authors.lastname: Park
authors.forename: Hong-Seob
authors.initials: HS
authors.lastname: So
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects / Q000502:physiology; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology*; D003051:Cochlea / Q000187:drug effects / Q000378:metabolism; D004781:Environmental Exposure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000473:pathology; D006309:Hearing / Q000187:drug effects; D006317:Hearing Loss, Noise-Induced / Q000473:pathology / Q000517:prevention & control* / Q000523:psychology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D007191:Indazoles / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009622:Noise / Q000009:adverse effects
chemical_list: D004791:Enzyme Inhibitors; C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D007191:Indazoles; C090937:3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole; D003035:Cobalt; D006162:Guanylate Cyclase; C018021:cobaltous chloride