title: [Biodegradation of o-chlorophenol by photosynthetic bacteria under co-metabolism].
abstract: Photosynthetic bacterial strain PSB-1D cannot utilize o-chlorophenol (2-CP) as the sole carbon source for energy. In this paper, different carbon sources (malic acid, sodium propionate, sodium acetate, sodium citrate, phenol, glucose, and soluble starch) were taken as the co-metabolism substrates to study their effects on PSB-1D growth and 2-CP degradation under the condition of aerobic culture in darkness. Among the substrates, glucose was most efficient, which promoted the reproduction of PSB-1D, enhanced the 2-CP degradation efficiency, and shortened the degradation period. The optimization experiment of added concentration of glucose showed that when the added glucose concentration was 3 g x L(-1), the PSB-1D cell concentration deltaD560 after 168 h culture was 1.749, the half-time of 2-CP was shortened to 3.9 d, and the degradation rate constant was increased to 0.00864 h(-1). The SDS-PAGE analysis on the total microbial cellular protein showed that taking glucose as the co-metabolism substrate, PSB-1D could induce a specific 2-CP-degrading enzyme.
authors.affiliation: College of Biological and Environmental Engineering, Shenyang University, Shenyang 110044, China. harvesttime@163.com
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Dong
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Hu
authors.forename: Ying-Dian
authors.initials: YD
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000302:isolation & purification* / Q000378:metabolism; D004785:Environmental Pollutants / Q000302:isolation & purification* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D011422:Propionates / Q000378:metabolism; D012241:Rhodopseudomonas / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002733:Chlorophenols; D004785:Environmental Pollutants; D008293:Malates; D011422:Propionates; C030298:malic acid; C514135:sodium propionate; D005947:Glucose; C030683:2-chlorophenol