title: [Matrix activity of mRNP of different cell localization from plants infected by minus-genome curly potato dwarfness virus in protein-synthesizing system in vitro].
abstract: Using structures of mRNP of different cell localization isolated from plants infected by minus-genomecurly potato dwarfness virus (CPDV), it was established that synthesis of virus proteins in vitro is mainly realized on membrane-related and free polysomal mRNP capable to direct synthesis of proteins programmed in RNA in cell-free protein-synthesizing systems. The obtained results prove that synthesis of virus proteins is actively realized on membrane-related nonpolysomal mRNP - 23520 imp/min. An analogous distribution of matrix activity is observed also in the case when mRNA isolated from mRNP of different cell localization was introduced in protein-synthesizing system. The least matrix activity was observed in cytoplasmic nonpolysomal mRNPs which are low-active in the translation system in vitro - only 1890 imp/min. The function of free cytoplasmic nonpolysomal mRNP is apparently mainly reduced to the transport and reserve of genetic information in a cell.
authors.forename: N I
authors.initials: NI
authors.lastname: Parkhomenko
authors.forename: L F
authors.initials: LF
authors.lastname: Didenko
authors.forename: L O
authors.initials: LO
authors.lastname: Maksymenko
authors.forename: N S
authors.initials: NS
authors.lastname: Diachenko
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology*; D014176:Protein Biosynthesis; D012209:Rhabdoviridae / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D011198:Solanum tuberosum; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D012261:Ribonucleoproteins; D014764:Viral Proteins; C026452:messenger ribonucleoprotein