title: A minD mutant of enterohemorrhagic E. coli O157:H7 has reduced adherence to human epithelial cells.
abstract: Adherence to epithelial cells is a prerequisite for intestinal colonization by the bacterial pathogen, enterohemorrhagic Escherichia coli (EHEC). The deletion of minD, a cell division gene, in EHEC caused reduced adherence to human epithelioid cervical carcinoma (HeLa) and human colonic adenocarcinoma (Caco-2) cells as compared to wild-type. The minD mutant formed minicells and filaments owing to aberrant cytokinesis. Moreover, its ability to form microcolonies as typically seen in the co-cultures of wild-type with Caco-2 cells, was abolished. In conclusion, the present study highlights the importance of minD in regards to EHEC adherence to human epithelial cells.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.
authors.forename: Rajinder P
authors.initials: RP
authors.lastname: Parti
authors.forename: Debabrata
authors.initials: D
authors.lastname: Biswas
authors.forename: Monica
authors.initials: M
authors.lastname: Wang
authors.forename: Mingmin
authors.initials: M
authors.lastname: Liao
authors.forename: Jo-Anne R
authors.initials: JA
authors.lastname: Dillon
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism*; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000235:genetics / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D009154:Mutation
chemical_list: D029968:Escherichia coli Proteins; D000251:Adenosine Triphosphatases; C072069:MinD protein, E coli