title: Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment.
abstract: Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in vitro cultures both in adherent and/or in spheroid conditions in almost all cases analyzed. We characterized in greater detail two samples which showed the most efficient propagation in vitro. In these samples we also compared gene profiles of spheroid vs adherent cultures and identified a set of differentially expressed genes. Finally we achieved efficient tumor engraftment in recipient NOD/SCID mice, also upon inoculation of small number of cells, thus suggesting indirectly the presence of tumor initiating cells.
authors.affiliation: Department of Clinical and Molecular Medicine, University of Rome La Sapienza S Andrea Hospital, Rome, Italy. rita.mancini@uniroma1.it
authors.forename: Rita
authors.initials: R
authors.lastname: Mancini
authors.forename: Enrico
authors.initials: E
authors.lastname: Giarnieri
authors.forename: Claudia
authors.initials: C
authors.lastname: De Vitis
authors.forename: Donatella
authors.initials: D
authors.lastname: Malanga
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Roscilli
authors.forename: Alessia
authors.initials: A
authors.lastname: Noto
authors.forename: Emanuele
authors.initials: E
authors.lastname: Marra
authors.forename: Carmelo
authors.initials: C
authors.lastname: Laudanna
authors.forename: Pietro
authors.initials: P
authors.lastname: Zoppoli
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Luca
authors.forename: Andrea
authors.initials: A
authors.lastname: Affuso
authors.forename: Luigi
authors.initials: L
authors.lastname: Ruco
authors.forename: Arianna
authors.initials: A
authors.lastname: Di Napoli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Mesiti
authors.forename: Luigi
authors.initials: L
authors.lastname: Aurisicchio
authors.forename: Alberto
authors.initials: A
authors.lastname: Ricci
authors.forename: Salvatore
authors.initials: S
authors.lastname: Mariotta
authors.forename: Lara
authors.initials: L
authors.lastname: Pisani
authors.forename: Claudio
authors.initials: C
authors.lastname: Andreetti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Viglietto
authors.forename: Erino A
authors.initials: EA
authors.lastname: Rendina
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Giovagnoli
authors.forename: Gennaro
authors.initials: G
authors.lastname: Ciliberto
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000369:Aged, 80 and over; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002448:Cell Adhesion; D002469:Cell Separation; D019295:Computational Biology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D016066:Pleural Effusion, Malignant / Q000235:genetics* / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D000444:Aldehyde Dehydrogenase