title: The thymus and tail regenerative capacity in Xenopus laevis tadpoles.
abstract: A morphofunctional analysis of the thymus from differently aged Xenopus laevis tadpoles during regeneration of the tail is reported. In stage 50 larvae, competent to regenerate, the appendage cut provoked thymic structural modifications that affected the medullary microenvironment cells and changes in TNF-α immunoreactivity. Mucocyte-like cells, multicellular epithelial cysts, myoid cells and cells immunoreactive to TNF-α increased in number. Increased numbers of lymphocytes were also found in regenerating areas and, at the end of regeneration, thymic structural and immunocytochemical patterns were restored to control levels. The observed cellular responses and the induction of molecules critical for thymus constitutive processes suggest a stimulation of thymic function after tail amputation. In older larvae, whose capacity to form a new complete and correctly patterned tail was reduced, thymic morphological changes were more severe and may persist throughout the regeneration process with a significant reduction in organ size. In these larvae the histological patterns and the marked thymic decrease may be related to the events occurring during regeneration, i.e. the higher inflammatory response and the reduced tail regenerative potential.
authors.affiliation: Department of Biology, University of Modena and Reggio Emilia, Italy. antonella.franchini@unimore.it
authors.forename: Antonella
authors.initials: A
authors.lastname: Franchini
authors.forename: Evelina
authors.initials: E
authors.lastname: Bertolotti
mesh_terms: D000367:Age Factors; D000671:Amputation, Surgical; D000818:Animals; D002452:Cell Count; D060833:Cellular Microenvironment; D007150:Immunohistochemistry; D007814:Larva / Q000033:anatomy & histology*; D008214:Lymphocytes / Q000166:cytology; D009929:Organ Size; D012038:Regeneration / Q000502:physiology*; D013194:Staining and Labeling; D013623:Tail / Q000293:injuries* / Q000502:physiology; D013950:Thymus Gland / Q000033:anatomy & histology* / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014982:Xenopus laevis / Q000033:anatomy & histology* / Q000293:injuries / Q000502:physiology
chemical_list: D014409:Tumor Necrosis Factor-alpha