title: Kinetin supplementation modifies chromium (VI) induced alterations in growth and ammonium assimilation in pea seedlings.
abstract: In the present study, impact of kinetin (KN; 10 and 100 μM) supplementation on growth, ammonium (NH(4)(+)) assimilation and antioxidant system in pea under hexavalent chromium toxicity (Cr VI; 50, 100 and 250 μM) was investigated. Chromium decreased growth, protein, and nitrogen, and activity of glutamine synthetase (GS) and glutamate synthase (GOGAT) while it increased NH(4)(+) content and activity of glutamate dehydrogenase (GDH). Kinetin at 100 μM decreased growth and NH(4)(+) assimilation, and together with Cr, it increased Cr toxicity. Chromium and 100 μM KN increased superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities while decreasing activities of catalase (CAT), glutathione reductase (GR) and dehydroascorbate reductase (DHAR). Ascorbate and glutathione levels were decreased by Cr and 100 μM KN. In contrast, supplementation of 10 μM KN under Cr (VI) toxicity, protected NH(4)(+) assimilation and promoted growth of pea by increasing levels of some of the antioxidants i.e., CAT, GR, DHAR, ascorbate and glutathione. Results showed that 10 μM KN increases Cr tolerance while 100 μM KN exhibited opposite responses. These results could contribute to an understanding of the mechanisms of KN-mediated dual influence on metal tolerance in crop plants.
authors.affiliation: Department of Plant Science, M.J.P. Rohilkhand University, Bareilly, 243006, India. gangwar.savi@gmail.com
authors.forename: Savita
authors.initials: S
authors.lastname: Gangwar
authors.forename: Vijay Pratap
authors.initials: VP
authors.lastname: Singh
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Garg
authors.forename: Sheo Mohan
authors.initials: SM
authors.lastname: Prasad
authors.forename: Jagat Narayan
authors.initials: JN
authors.lastname: Maurya
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000032:analysis / Q000378:metabolism; D002857:Chromium / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D007701:Kinetin / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D018532:Pisum sativum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D018520:Plant Shoots / Q000737:chemistry / Q000254:growth & development; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D002857:Chromium; D005978:Glutathione; D009584:Nitrogen; D007701:Kinetin; D001205:Ascorbic Acid