title: Chemical structures and hepatoprotective effects of constituents from the leaves of Salacia chinensis.
abstract: The methanolic extract from the leaves of Salacia chinensis collected in Thailand was found to show a protective effect on D-galactosamine-induced cytotoxicity in primary cultured mouse hepatocytes. From the methanolic extract, eight new glycosides, named foliachinenosides E, F, G, H, and I, and foliasalaciosides J, K and L, were isolated together with 26 known constituents. The structures of new glycosides were determined on the basis of physicochemical and chemical evidence. In addition, the hepatoprotective effects of the isolated compounds on D-galactosamine-induced cytotoxicity were examined. Among them, lignans, eleutheroside E₂ and 7R,8S-dihydrodehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside, were found to show the protective effects [inhibition (%) 41.4 ± 3.6 (p < 0.01), 45.5 ± 2.7 (p < 0.01) at 100 µM, respectively].
authors.affiliation: Kyoto Pharmaceutical University, Kyoto, Japan.
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Osamu
authors.initials: O
authors.lastname: Muraoka
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D049918:Salacia / Q000737:chemistry*; D013785:Thailand
chemical_list: D005960:Glucosides; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; C459217:eleutheroside E(2)