title: Development of interdigitated arrays coated with functional polyaniline/MWCNT for electrochemical biodetection: application for human papilloma virus.
abstract: In this study, polyaniline-multiwalled carbon nanotube film (PANi-MWCNT) has been polymerized on interdigitated platinum electrode arrays (IDA), fabricated by MEMS technology for the detection of human papillomavirus (HPV) infection, using immobilized peptide aptamers as affinity capture reagent. Label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer HPV-16-L1 (with a molecular weight of 1825 Da), the most common genotype in cytological normal women worldwide, and its specific antibody of HPV-16 (which is much bigger with molecular weight of ca. 150 kDa) on multifunctional PANi-MWCNT based arrays was reported. The most significant advantage of this technique consists of reagentless and multiple detection of antigen-antibody complex formation on well conducting IDA interface of PANi-MWCNT, without intermediate steps or any labeling reagents, as normally required in the previous works.
authors.affiliation: Institute of Materials Science, Vietnam Academy of Science and Technology, Ha Noi, Viet Nam. lamtd@ims.vast.ac.vn
authors.forename: Lam Dai
authors.initials: LD
authors.lastname: Tran
authors.forename: Dzung Tuan
authors.initials: DT
authors.lastname: Nguyen
authors.forename: Binh Hai
authors.initials: BH
authors.lastname: Nguyen
authors.forename: Quan Phuc
authors.initials: QP
authors.lastname: Do
authors.forename: Huy Le
authors.initials: HL
authors.lastname: Nguyen
mesh_terms: D000595:Amino Acid Sequence; D000814:Aniline Compounds / Q000737:chemistry*; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D052158:Aptamers, Peptide / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000276:immunology; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009856:Oncogene Proteins, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000821:virology
chemical_list: D000814:Aniline Compounds; D000914:Antibodies, Viral; D000956:Antigens, Viral; D052158:Aptamers, Peptide; D036022:Capsid Proteins; D037742:Nanotubes, Carbon; D009856:Oncogene Proteins, Viral; C416807:polyaniline; C063317:L1 protein, Human papillomavirus type 16