title: Colloidal properties of biodegradable nanoparticles influence interaction with amyloid-β peptide.
abstract: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the extracellular deposition of amyloid-β peptides (Aβ). During the past few years, promising approaches based on nanotechnologies have emerged to alter Aβ aggregation and its related toxicity. This study aims to investigate the influence of the nanoparticle colloidal properties over the interaction with Aβ peptide 1-42 (Aβ(1-42)). Using capillary electrophoresis with laser-induced fluorescence detection, it was shown that biodegradable poly(ethylene glycol)-block-polylactide (PEG-b-PLA) nanoparticles were able to interact with Aβ(1-42) peptide leading to its uptake in rather short time periods. In addition, we highlighted the crucial role of the nanocarrier colloidal properties on the uptake kinetics. Whereas nanoparticles stabilized by sodium cholate (lower size and higher negative surface charge) gave optimum uptake kinetics, nanoparticles stabilized with others surfactants presented lower interactions. In contrast, PEG density seemed to have no influence on the interaction when sodium cholate was used for the preparation. This study intends to give new insights into Aβ(1-42) peptide interaction with nanoparticulate systems by helping to determine suitable nanoparticle characteristics regarding forthcoming therapeutic strategies against AD.
authors.affiliation: Laboratoire de Physico-Chimie, Pharmacotechnie et Biopharmacie, Univ. Paris-Sud, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, Châtenay-Malabry cedex, France.
authors.forename: Davide
authors.initials: D
authors.lastname: Brambilla
authors.forename: Hayfa
authors.initials: H
authors.lastname: Souguir
authors.forename: Julien
authors.initials: J
authors.lastname: Nicolas
authors.forename: Nicolas
authors.initials: N
authors.lastname: Mackiewicz
authors.forename: Romain
authors.initials: R
authors.lastname: Verpillot
authors.forename: Benjamin
authors.initials: B
authors.lastname: Le Droumaguet
authors.forename: Myriam
authors.initials: M
authors.lastname: Taverna
authors.forename: Patrick
authors.initials: P
authors.lastname: Couvreur
authors.forename: Karine
authors.initials: K
authors.lastname: Andrieux
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D003102:Colloids / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D020358:Sodium Cholate / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D003102:Colloids; D010446:Peptide Fragments; D011091:Polyesters; D013501:Surface-Active Agents; C075222:amyloid beta-protein (1-42); D011092:Polyethylene Glycols; C033616:poly(lactide); D020358:Sodium Cholate