title: Quantitative proteomics identify novel miR-155 target proteins.
abstract: BACKGROUND
  MicroRNAs are 22 nucleotides long non-coding RNAs and exert their function either by transcriptional or translational inhibition. Although many microRNA profiles in different tissues and disease states have already been discovered, only little is known about their target proteins. The microRNA miR-155 is deregulated in many diseases, including cancer, where it might function as an oncoMir.
  
  
  RESULTS
  We employed a proteomics technique called "stable isotope labelling by amino acids in cell culture" (SILAC) allowing relative quantification to reliably identify target proteins of miR-155. Using SILAC, we identified 46 putative miR-155 target proteins, some of which were previously reported. With luciferase reporter assays, CKAP5 was confirmed as a new target of miR-155. Functional annotation of miR-155 target proteins pointed to a role in cell cycle regulation.
  
  
  CONCLUSIONS
  To the best of our knowledge we have investigated for the first time miR-155 target proteins in the HEK293T cell line in large scale. In addition, by comparing our results to previously identified miR-155 target proteins in other cell lines, we provided further evidence for the cell line specificity of microRNAs.
authors.affiliation: Functional Proteome Analysis, German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Christopher
authors.initials: C
authors.lastname: Lössner
authors.forename: Jan
authors.initials: J
authors.lastname: Meier
authors.forename: Uwe
authors.initials: U
authors.lastname: Warnken
authors.forename: Michael A
authors.initials: MA
authors.lastname: Rogers
authors.forename: Peter
authors.initials: P
authors.lastname: Lichter
authors.forename: Armin
authors.initials: A
authors.lastname: Pscherer
authors.forename: Martina
authors.initials: M
authors.lastname: Schnölzer
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western; D015536:Down-Regulation / Q000235:genetics; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007553:Isotope Labeling; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000596:Amino Acids; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D011506:Proteins