title: Can 17-β estradiol induce vitellogenin-like proteins in the clam Tapes philippinarum?
abstract: Estrogenic potency of 17-β estradiol (E2) was evaluated for the first time in both sexually undifferentiated (during resting phase, at the very early stage of gametogenesis) and differentiated (in pre-spawning phase) clams (Tapes philippinarum), following waterborne exposure for 7 and 14 days to various E2 concentrations (0, 0+acetone, 5, 25, 50, 100 and 1000ng/L). Vitellogenin (Vg)-like protein levels were evaluated in both haemolymph and digestive glands by the alkali-labile phosphate (ALP) assay. In the resting phase of clams, exposure for 7 days to all E2 concentrations tested resulted in significant increases in ALP in haemolymph with respect to controls. Conversely, no statistically significant differences were observed in digestive gland from E2-exposed bivalves, compared with controls. After 14-days exposure, ALP significantly increased in haemolymph from 1000ng E2/L-exposed clams and in digestive gland from 5ng E2/L-exposed animals. Interestingly, in the pre-spawning phase of clams, significant decreases in ALP levels were recorded in haemolymph from females exposed for 7 days, whereas no differences were observed in males. No significant variations were observed in ALP content in digestive gland from both females and males. ALP levels in haemolymph were shown to increase significantly in females exposed for 14 days to 50ng E2/L, and in males exposed to 100ng E2/L. Exposure for 14 days to all the E2 concentrations tested resulted in significant increases of ALP levels in the digestive gland of females. Conversely, variations in ALP content of male digestive glands were not significant. Results obtained appeared controversial and somewhat difficult to explain. Indeed, no univocal response by clams was observed after 7 and 14 days exposure to E2. Further studies need to elucidate the role of E2 in promoting Vg induction in clams.
authors.affiliation: Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Matozzo
authors.forename: Maria Gabriella
authors.initials: MG
authors.lastname: Marin