title: Latency delay of visual evoked potential is a real measurement of demyelination in a rat model of optic neuritis.
abstract: OBJECTIVE
  To investigate the relationship between size of demyelinated lesion, extent of axonal loss, and degree of latency delay of visual evoked potentials (VEPs) in a rat model of experimental demyelination.
  
  
  METHODS
  Lysolecithin 1% (0.4 or 0.8 μL) was microinjected into an optic nerve of each of 14 rats 2 mm posterior to the globe. Standard flash VEPs were recorded with skull-implanted electrodes before and 2, 4, and 6 days after the microinjection. The optic nerves were stained with Luxol-fast blue and Bielschowsky's silver to assess demyelination and axonal pathology, respectively. Demyelinated areas were measured on serial sections, and lesion volumes were deduced by three-dimensional reconstruction.
  
  
  RESULTS
  Focal lesions of demyelination and variable axonal loss were observed. The mean volume of the lesion was 3.2 ± 1.1 × 10⁻² mm³. The injected eye showed a significant latency delay and amplitude decrease. Regression analysis demonstrated a strong correlation between N1 latency delay and lesion volume (r = 0.863, P < 0.0001), which remained significant after adjustment for axonal loss (r = 0.829, P < 0.001). N1 latency delay also showed a correlation with axonal loss (r = 0.552, P = 0.041), but the correlation became nonsignificant when controlling for demyelination (r = 0.387, P = 0.191). A linear association between N1-P2 amplitude decrease and axonal loss (r = 0.681, P = 0.007) was also observed.
  
  
  CONCLUSIONS
  The latency of the VEP accurately reflected the amount of demyelination in the visual pathway, whereas the amplitude correlated with axonal damage. This study supports the concept that the VEP provides a highly sensitive tool with which to measure demyelination in optic neuritis.
authors.affiliation: Department of Ophthalmology, Australian School of Advanced Medicine, Macquarie University, Sydney, Australia. yuyi.you@gmail.com
authors.forename: Yuyi
authors.initials: Y
authors.lastname: You
authors.forename: Alexander
authors.initials: A
authors.lastname: Klistorner
authors.forename: Johnson
authors.initials: J
authors.lastname: Thie
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Graham
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D003711:Demyelinating Diseases / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D004596:Electroretinography; D005074:Evoked Potentials, Visual / Q000502:physiology*; D008244:Lysophosphatidylcholines / Q000633:toxicity; D008297:Male; D008845:Microinjections; D009900:Optic Nerve / Q000473:pathology* / Q000503:physiopathology; D009902:Optic Neuritis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index
chemical_list: D008244:Lysophosphatidylcholines