title: Enantiomer elution order reversal of fluorenylmethoxycarbonyl-isoleucine in high-performance liquid chromatography by changing the mobile phase temperature and composition.
abstract: In this paper the elution order reversal of enantiomers of fluorenylmethoxycarbonyl- or FMOC-isoleucine is described depending on the separation temperature and composition of the mobile phase when using the polysaccharide-based chiral column Lux Cellulose-1 in HPLC with normal-phase eluent. Reversal of the enantiomer elution order (EEO) in HPLC depending on the column temperature and content of the polar modifier in the mobile phase has been reported before in the literature. However, EEO reversal by changing the content of acidic modifier in the mobile phase seems to be described for the first time in the present work.
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: L
authors.initials: L
authors.lastname: Chankvetadze
authors.forename: N
authors.initials: N
authors.lastname: Ghibradze
authors.forename: M
authors.initials: M
authors.lastname: Karchkhadze
authors.forename: L
authors.initials: L
authors.lastname: Peng
authors.forename: T
authors.initials: T
authors.lastname: Farkas
authors.forename: B
authors.initials: B
authors.lastname: Chankvetadze
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes / Q000737:chemistry*; D007532:Isoleucine / Q000031:analogs & derivatives* / Q000737:chemistry; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D007532:Isoleucine; D002482:Cellulose