title: Tissue-specific knockout of androgen receptor in mice.
abstract: Androgen acting through the androgen receptor (AR) is known to be essential for male sexual differentiation and development. Using Cre-lox technology, we have generated the floxed AR mice, which have been bred with general or tissue-specific Cre expressing transgenic mice to knock out the AR gene in specific target cells. Our findings indicated that AR is required for sexual development and that loss of AR can have significant effects on many aspects of physiological functions and disease progression, such as immune function, metabolism, and tumorigenesis. Furthermore, our strategy can generate AR knockout (ARKO) in female mice, which allows researchers to study the AR function in the female. In brief, our floxed AR mouse model provides a powerful tool to study in vivo AR functions in selective tissues and cell types and has made possible several research breakthroughs in the field of endocrinology.
authors.affiliation: George H. Whipple Lab, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642-8626, USA. tzu-hua_lin@urmc.rochester.edu
authors.forename: Tzu-hua
authors.initials: TH
authors.lastname: Lin
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Yeh
authors.forename: Chawnshang
authors.initials: C
authors.lastname: Chang
mesh_terms: D000818:Animals; D001947:Breeding; D002478:Cells, Cultured; D053595:Embryonic Stem Cells; D005260:Female; D055786:Gene Knockout Techniques; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D058889:Molecular Typing; D009928:Organ Specificity / Q000235:genetics; D011944:Receptors, Androgen / Q000235:genetics*
chemical_list: D011944:Receptors, Androgen