title: [Identification of catalytically active groups of pea (Pisum sativum L.) beta-glucosidase].
abstract: Functional groups ofcytoplasmic pea beta-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzyme with diethyl pyrocarbonate suggest that the catalytic site of beta-glucosidase contains the carboxyl group of glutamic or aspartic acids and the imidazole group of histidine.
authors.forename: A N
authors.initials: AN
authors.lastname: Ershova
authors.forename: O N
authors.initials: ON
authors.lastname: Barkalova
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry; D001665:Binding Sites; D020134:Catalytic Domain; D004047:Diethyl Pyrocarbonate / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D018698:Glutamic Acid / Q000737:chemistry; D006639:Histidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007700:Kinetics; D008751:Methylene Blue / Q000494:pharmacology; D018532:Pisum sativum / Q000737:chemistry / Q000201:enzymology*; D010782:Photolysis / Q000187:drug effects; D013816:Thermodynamics; D001617:beta-Glucosidase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007093:Imidazoles; D001224:Aspartic Acid; D018698:Glutamic Acid; D006639:Histidine; C029899:imidazole; D001617:beta-Glucosidase; D004047:Diethyl Pyrocarbonate; D008751:Methylene Blue