title: Protective effect of Withania somnifera and Cardiospermum halicacabum extracts against collagenolytic degradation of collagen.
abstract: The irreversible destruction of extracellular matrix (ECM) such as cartilage, tendon, and bone that comprise synovial joints is the hallmark of both rheumatoid arthritis and osteoarthritis by over-expression of matrix metalloproteinase (MMP)-collagenases. We report herein the detailed study on the inhibitory effects of Withania somnifera extract (WSE) and Cardiospermum halicacabum extract (CHE) on Clostridium histolyticum collagenase (ChC) activity against the degradation of the ECM component of bovine Achilles tendon type I collagen by hydroxyproline assay method. Interaction of WSE and CHE with ChC exhibited 71% and 88% inhibition, respectively, to the collagenolytic activity of ChC against collagen degradation, and the inhibition was found to be concentration-dependent. The inhibition kinetics of ChC by both the extracts has been deduced from the extent of hydrolysis of N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala. Both WSE and CHE are provided competitive and mixed type inhibition on ChC activity, respectively. Circular dichroism studies of ChC on treatment with WSE and CHE revealed changes in the secondary structure of collagenase. These results suggest that the WSE and CHE facilitated collagen stabilization through collagenase inhibition.
authors.affiliation: Bioproducts Laboratory, Central Leather Research Institute (Council of Scientific and Industrial Research), Adyar, Chennai, 600 020 Tamil Nadu, India. krishnamoorthyganesh@yahoo.com
authors.forename: Krishnamoorthy
authors.initials: K
authors.lastname: Ganesan
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Sehgal
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
authors.forename: Sadulla
authors.initials: S
authors.lastname: Sayeed
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology; D002356:Cartilage / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D002417:Cattle; D002942:Circular Dichroism; D024042:Collagen Type I / Q000737:chemistry / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D006868:Hydrolysis; D006909:Hydroxyproline / Q000032:analysis; D007700:Kinetics; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D017433:Protein Structure, Secondary; D029631:Sapindaceae / Q000737:chemistry*; D012996:Solutions; D032341:Withania / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D010936:Plant Extracts; D011480:Protease Inhibitors; D012996:Solutions; D003012:Microbial Collagenase; D006909:Hydroxyproline