title: Histopathological and proteomic analysis of hepatic tissue from adult male zebrafish exposed to 17β-estradiol.
abstract: 17β-Estradiol (E2) is known to contribute significantly a large extent to the estrogenicity in aquatic system. In the present study, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MALDI-TOF-TOF MS), combined with histopathological analysis, was used to screen hepatic responses in adult male zebrafish. Eight proteins were found to be up-regulated more than 2-fold, whereas five protein spots were down-regulated more than 2-fold after 1nM E2 treatment for 14 days, which had caused histological effects in zebrafish livers. These differentially expressed proteins accounted for a variety of cellular biological processes, such as response to oxidative stress, cell surface receptor-linked signal transduction, oxidation-reduction and cellular calcium ion homeostasis. The results demonstrated that E2 comprehensively influenced a variety of cellular and biological processes in zebrafish. Moreover, the proteomic responses elicited in zebrafish allow us to better understand the underlying mechanisms of endocrine-disrupting chemicals (EDCs)-induced toxicity fully.
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Yuanxiang
authors.initials: Y
authors.lastname: Jin
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Dezhao
authors.initials: D
authors.lastname: Lu
authors.forename: Liwei
authors.initials: L
authors.lastname: Sun
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qian
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Fu