title: Simultaneous determination of 12 beta-lactam antibiotics in human plasma by high-performance liquid chromatography with UV detection: application to therapeutic drug monitoring.
abstract: A rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 μg/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.
authors.affiliation: Laboratoire de Pharmacologie Biologique, Hôpital de Pontchaillou, 2 Rue Henri le Guilloux, 35033 Rennes Cedex 9, France. marieclemence.verdier@chu-rennes.fr
authors.forename: Marie-Clémence
authors.initials: MC
authors.lastname: Verdier
authors.forename: Olivier
authors.initials: O
authors.lastname: Tribut
authors.forename: Pierre
authors.initials: P
authors.lastname: Tattevin
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Tulzo
authors.forename: Christian
authors.initials: C
authors.lastname: Michelet
authors.forename: Danièle
authors.initials: D
authors.lastname: Bentué-Ferrer
mesh_terms: D000900:Anti-Bacterial Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D047090:beta-Lactams / Q000097:blood*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams