title: Studies of inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by using a visible femtosecond laser: insight into the possible inactivation mechanisms.
abstract: We report experimental results on the inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by a visible femtosecond laser. Our results suggest that inactivation of virus and bacterium by a visible femtosecond laser involves completely different mechanisms. Inactivation of viruses by a visible femtosecond laser involves the breaking of hydrogen∕hydrophobic bonds or the separation of the weak protein links in the protein shell of a viral particle. In contrast, inactivation of bacteria is related to the damage of their DNAs due to irradiation of a visible femtosecond laser. Possible mechanisms for the inactivation of viruses and bacteria are discussed.
authors.affiliation: Arizona State University, Department of Physics, Tempe, Arizona 85287, USA. tsen@asu.edu
authors.forename: Kong T
authors.initials: KT
authors.lastname: Tsen
authors.forename: Shaw-Wei D
authors.initials: SW
authors.lastname: Tsen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Lindsay
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Stephanie
authors.initials: S
authors.lastname: Cope
authors.forename: Sara
authors.initials: S
authors.lastname: Vaiana
authors.forename: Juliann G
authors.initials: JG
authors.lastname: Kiang
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000528:radiation effects*; D002417:Cattle; D002942:Circular Dichroism; D004279:DNA, Viral / Q000528:radiation effects; D004686:Encephalomyocarditis virus / Q000528:radiation effects*; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D018625:Microscopy, Atomic Force; D036641:Microscopy, Fluorescence, Multiphoton; D012486:Salmonella typhimurium / Q000528:radiation effects*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000528:radiation effects; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman; D014771:Virion / Q000528:radiation effects
chemical_list: D004279:DNA, Viral; D012710:Serum Albumin, Bovine