title: [Molecular and epidemiological study on viral diarrhea among infants in Lanzhou].
abstract: OBJECTIVE
  To study the epidemiologic characteristics of viral diarrhea in children under 5 years old in Lanzhou, understand the four major virus in children of distribution.
  
  
  METHODS
  In the first hospital of Lanzhou university from Jul 2009 to Jun 2010,we collected 290 stool specimens from children with diarrhea and 114 asymptomatic controls. Rotavirus was detected by ELISA,further strain characterization was carried out by nested PCR. The human calicivirus, astrovirus, adenovirus were detected by RT-multiplex PCR and PCR.
  
  
  RESULTS
  At least one of the four viral agents was found in 60% of the specimens. Rotavirus, human calicivirus, adenovirus, and astrovirus were identified in 39.31%, 11.38%, 10.69%, and 4.83% in 290 specimens respectively. Rotavirus G3 was the most prevailing serotype, P [8] was the most common genotype. In the 114 control samples, 7 sample was positived for calicivirus, 5 samples were positived for human adenovirus and 1 sample was positived for astrovirus.
  
  
  CONCLUSIONS
  The results indicated clearly the impact of viral agents causing diarrhea and the importance of long-term systematic surveillance.
authors.affiliation: First Clinical Medical College of Lanzhou University, Lanzhou 730000, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Luo
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Yu-ning
authors.initials: YN
authors.lastname: Li
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Li
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Ye-xia
authors.initials: YX
authors.lastname: Hao
authors.forename: Yong-xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Cui
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification; D002139:Caliciviridae / Q000302:isolation & purification; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D001257:Mamastrovirus / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification