title: Silk fibroin has a protective effect against high glucose induced apoptosis in HIT-T15 cells.
abstract: High glucose levels induce cell death in many cell types, including pancreatic β-cells. Although protective agents against glucotoxicity have been searched for extensively, so far none have been found. In this report, we tested silk fibroin (SF) as a candidate material for antiglucotoxicity in the pancreatic β-cell (HIT-T15 cell) line. Approximately 50% of cells were killed after treatment with 80 mg/mL glucose. This reduction of cell number was recovered by the addition of SF at 50 mg/mL. SF treatment also decreased cellular reactive oxygen species (ROS) and increased proliferating cellular nuclear antigen (PCNA) immunoreactivity. In addition, TUNEL assays demonstrated that SF protects against glucose-induced apoptosis of HIT-T15 cells, suggesting that SF might protect cells from cell death by lowering cellular ROS levels. SF also induced expression of the insulin-like growth factor-1 (IGF-1) gene, and IGF-1 expression may be the cause of SF-induced protection against glucose toxicity. Taken together, these results suggest that SF could serve as a potential therapeutic agent to treat the hyperglycemia-induced death of pancreatic β-cells.
authors.affiliation: Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, Gangwon-do 200-702, Republic of Korea.
authors.forename: Jun Hong
authors.initials: JH
authors.lastname: Park
authors.forename: YoonYi
authors.initials: Y
authors.lastname: Nam
authors.forename: So-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Jin-Kyung
authors.initials: JK
authors.lastname: Kim
authors.forename: Nong-Hoon
authors.initials: NH
authors.lastname: Choe
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Yang-Seok
authors.initials: YS
authors.lastname: Oh
authors.forename: Jun Gyo
authors.initials: JG
authors.lastname: Suh
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D019610:Cytoprotection; D005349:Fibroins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000502:physiology / Q000633:toxicity*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D007334:Insulin-Like Growth Factor I; D005349:Fibroins; D005947:Glucose