title: Expression and subcellular localization of apical junction proteins in canine duodenal and colonic mucosa.
abstract: OBJECTIVE
  To examine the expression and distribution of tight junction (TJ) and adherens junction (AJ) proteins in canine duodenal and colonic mucosa.
  
  
  METHODS
  Mucosa obtained from 4 healthy Beagles.
  
  
  METHODS
  Biopsy specimens of the duodenum and colon were obtained via endoscopy from 4 healthy dogs. The expression patterns and subcelluar localization of claudin-1, -2, -3, -4, -5, -7, and -8; E-cadherin; and β-catenin in the duodenum and colon were analyzed by use of immunoblotting and immunofluorescence microscopy.
  
  
  RESULTS
  In the duodenum, there was clear expression of claudin-3 and -5, E-cadherin, and β-catenin proteins and weak expression of claudin-7 protein. In contrast, there was clear expression of claudin-2 and -3, E-cadherin, and β-catenin proteins and weak expression of claudin-5 and -7 proteins in the colon, as determined by use of immunoblotting. As determined by the use of immunofluorescence microscopy, the duodenum and colon had staining for claudin-3 and -5, E-cadherin, and β-catenin in the most apical region and staining for claudin-7 in the basolateral region. Staining for claudin-2 was also observed in the colon.
  
  
  CONCLUSIONS
  Information was provided about the expression patterns of TJ and AJ proteins in the duodenum and colon of clinically normal dogs. These results may provide valuable information for use in evaluating the importance of these TJ and AJ proteins in the pathogenesis of inflammatory bowel disease in dogs.
authors.affiliation: Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan. h-ohta@vetmed.hokudai.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Tomoki
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: B K Wickramasekara
authors.initials: BK
authors.lastname: Rajapakshage
authors.forename: Masahiro
authors.initials: M
authors.lastname: Murakami
authors.forename: Noboru
authors.initials: N
authors.lastname: Sasaki
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Shiang-Jyi
authors.initials: SJ
authors.lastname: Hwang
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Takiguchi
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D001706:Biopsy / Q000662:veterinary; D015820:Cadherins / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting / Q000662:veterinary; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000662:veterinary; D019108:Tight Junctions / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D015820:Cadherins; D008565:Membrane Proteins; D051176:beta Catenin