title: Construction of three different recombinant scorpion fusion proteins with bifunctional activity.
abstract: This is the first report of three different fusion proteins with an antitumor-analgesic peptide obtained from Chinese scorpion Buthus martensii Karsch (BmKAGAP). The fusion proteins were constructed in the form of chimeric toxins, aiming to obtain bifunctional analgesic and antitumor activity. The fusion proteins consisted of luteinizing hormone-releasing hormone (LHRH), three different types of flexible linkers (L1, Ser-Ser-His-His-His-His-His-His-Ser-Ser-Gly-Leu-Val-Pro-Arg-Gly-Ser-His-Met; L2, Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser; L3, Ser-Gly-Gly-Ser-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Ser-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser), and BmKAGAP. The genes coding three fusion proteins were cloned and expressed in E. coli in soluble form. Following two successive column chromatographic separations, purified fusion proteins were obtained. These fusion proteins exhibited analgesic activity in mice and were cytotoxic to a hepatocellular carcinoma cell line Hep3B.
authors.affiliation: School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, P.O. Box 17, 103 Wenhua Road, Shenhe District, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.forename: G L
authors.initials: GL
authors.lastname: Guo
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Mao
authors.forename: R
authors.initials: R
authors.lastname: Zhang
authors.forename: C F
authors.initials: CF
authors.lastname: Wu
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D012604:Scorpion Venoms / Q000008:administration & dosage / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012605:Scorpions
chemical_list: D000700:Analgesics; D000970:Antineoplastic Agents; D011993:Recombinant Fusion Proteins; D012604:Scorpion Venoms; C477804:antitumor-analgesic peptide, Buthus martensii