title: Iron(II) binding to amyloid-β, the Alzheimer's peptide.
abstract: Iron has been implicated in Alzheimer's disease, but until now no direct proof of Fe(II) binding to the amyloid-β peptide (Aβ) has been reported. We used NMR to evidence Fe(II) coordination to full-length Aβ40 and truncated Aβ16 peptides at physiological pH and to show that the Fe(II) binding site is located in the first 16 amino-acid residues. Fe(II) caused selective broadening of some NMR peaks that was dependent on the Fe:Aβ stoichiometry and temperature. Analysis of Fe(II) broadening effect in the (1)H, (13)C, and 2D NMR data established that Asp1, Glu3, the three His, but not Tyr10 nor Met35 are the residues mainly involved in Fe(II) coordination.
authors.affiliation: CNRS , LCC (Laboratoire de Chimie de Coordination), 205 route de Narbonne, F-31077 Toulouse, France.
authors.forename: Fatima
authors.initials: F
authors.lastname: Bousejra-ElGarah
authors.forename: Christian
authors.initials: C
authors.lastname: Bijani
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coppel
authors.forename: Peter
authors.initials: P
authors.lastname: Faller
authors.forename: Christelle
authors.initials: C
authors.lastname: Hureau
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding
chemical_list: D016229:Amyloid beta-Peptides; D007501:Iron