title: Royal jelly protects against ultraviolet B-induced photoaging in human skin fibroblasts via enhancing collagen production.
abstract: Royal jelly (RJ) is a honeybee product containing proteins, carbohydrates, fats, free amino acids, vitamins, and minerals. As its principal unsaturated fatty acid, RJ contains 10-hydroxy-2-decenoic acid (10-HDA), which may have antitumor and antibacterial activity and a capacity to stimulate collagen production. RJ has attracted interest in various parts of the world for its pharmacological properties. However, the effects of RJ on ultraviolet (UV)-induced photoaging of the skin have not been reported. In this study we measured the 10-HDA content of RJ by high-performance liquid chromatography and tested the effects of RJ on UVB-induced skin photoaging in normal human dermal fibroblasts. The effects of RJ and 10-HDA on UVB-induced photoaging were tested by measuring procollagen type I, transforming growth factor (TGF)-β1, and matrix metalloproteinase (MMP)-1 after UVB irradiation. The RJ contained about 0.211% 10-HDA. The UVB-irradiated human skin fibroblasts treated with RJ and 10-HDA had increased procollagen type I and TGF-β1 productions, but the level of MMP-1 was not changed. Thus RJ may potentially protect the skin from UVB-induced photoaging by enhancing collagen production.
authors.affiliation: Department of Medical Science, Kyung Hee University, Seoul, Korea.
authors.forename: Hye Min
authors.initials: HM
authors.lastname: Park
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.forename: Kwang Gill
authors.initials: KG
authors.lastname: Lee
authors.forename: Sang-Mi
authors.initials: SM
authors.lastname: Han
authors.forename: Yunhi
authors.initials: Y
authors.lastname: Cho
authors.forename: Sun Yeou
authors.initials: SY
authors.lastname: Kim
mesh_terms: D056705:Apitherapy; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D024042:Collagen Type I / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000009:adverse effects / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D009994:Osmolar Concentration; D011347:Procollagen / Q000378:metabolism; D056910:Republic of Korea; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology; D013473:Sunscreening Agents / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D015854:Up-Regulation / Q000187:drug effects*; D000099219:Royal Jelly
chemical_list: D024042:Collagen Type I; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D020781:Matrix Metalloproteinase 1; D011347:Procollagen; D013473:Sunscreening Agents; D053773:Transforming Growth Factor beta1; D000099219:Royal Jelly; C508960:TGFB1 protein, human; C055543:10-hydroxy-2-decenoic acid; C522360:MMP1 protein, human