title: [Bioengineering of symbiotic systems: creation of new associative symbiosis with the use of lectins on the example of tobacco and colza].
abstract: "Barbate roots" in tobacco and colza transgenic on lectin gene were obtained with the use of a wild strain of Agrobacterium rhizogenes 15834 transformed with pCAMBIA1305.1 plasmid containing the full-size lectin gene (psl) from the Pisum sativum. Influence of expression oflectin gene on colonization oftransgenic roots with symbiont of pea (Rhizobium leguminosarum) was investigated. The number of adhered bacteria onto the roots transformed with lectin gene was 14-fold and 37-fold higher in comparison with the control; this confirms the interaction of R. leguminosarum with pea lectin at the surface of the transformed roots of tobacco and colza. The developed experimental approach, based on the simulation of recognition processes and early symbiotic interactions with lectins of pea plants, may, in perspective, be used for obtaining stable associations of economically valuable, nonsymbiotrophic plant species with rhizobia.
authors.forename: Z P
authors.initials: ZP
authors.lastname: Vershinina
authors.forename: An Kh
authors.initials: AKh
authors.lastname: Baĭmiev
authors.forename: D K
authors.initials: DK
authors.lastname: Blagova
authors.forename: A V
authors.initials: AV
authors.lastname: Kniazev
authors.forename: Al Kh
authors.initials: AKh
authors.lastname: Baĭmiev
authors.forename: A V
authors.initials: AV
authors.lastname: Chemeris
mesh_terms: D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D015169:Colony Count, Microbial; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods; D018532:Pisum sativum / Q000737:chemistry / Q000235:genetics; D037121:Plant Lectins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000737:chemistry / Q000235:genetics; D016961:Rhizobium leguminosarum / Q000254:growth & development*; D012639:Seeds / Q000737:chemistry / Q000235:genetics; D013559:Symbiosis / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000382:microbiology*; D014162:Transfection
chemical_list: D037121:Plant Lectins; C036292:pea lectin