title: Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin.
abstract: A colloidal gold conjugated anti-baicalin monoclonal antibody (anti-BA MAb) was prepared and used in an immunochromatographic assay (ICA) for BA in Scutellariae Radix and Kampo medicines. This competitive ICA uses an anti-BA MAb which shows a high specificity for BA and baicalein. Its advantages include a short assay time (15 min), no dependence on any instrumental systems, and it can detect BA in plant materials and Kampo medicines. The limit of detection for the ICA was found to be around 0.6 μg mL(-1)of baicalin. Moreover, the usefulness of the combination of indirect competitive ELISA and the ICA using anti-BA MAb as a quality control method was confirmed for analysis of BA in Scutellariae Radix and Kampo medicines with a sufficient sensitivity (200 ng mL(-1) to 2 μg mL(-1)), obtainable in an easy and timely manner.
authors.affiliation: Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
authors.forename: Madan Kumar
authors.initials: MK
authors.lastname: Paudel
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
authors.forename: Boonchoo
authors.initials: B
authors.lastname: Sritularak
authors.forename: Osamu
authors.initials: O
authors.lastname: Morinaga
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morimoto
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000276:immunology; D006046:Gold / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D020835:Medicine, Kampo; D031364:Scutellaria baicalensis / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D005419:Flavonoids; C038044:baicalin; D006046:Gold