title: Lipid composition regulates the conformation and insertion of the antimicrobial peptide maculatin 1.1.
abstract: Antimicrobial peptides interact with cell membranes and their selectivity is contingent on the nature of the constituent lipids. Eukaryotic and bacterial membranes are comprised of different proportions of a range of lipid species with different physical properties. Hence, characterisation of antimicrobial peptides with respect to the magnitude of their interactions with model membranes of different lipid types is needed. Maculatin 1.1 is a short antimicrobial peptide secreted from the skin of several Australian tree-frog species. Circular dichroism spectroscopy (CD) was used to explore the interaction of maculatin 1.1 with a wide range of model membrane systems of different head group and acyl chain characteristics. For neutral phosphatidylcholine (PC), unlike anionic phospholipids, the magnitude of the peptide interactions was dependent on the length and degree of saturation of the constituent acyl chains. Oriented circular dichroism (OCD) data indicated that helical structure was likely promoted by peptide insertion into the hydrophobic core of PC bilayers. The addition of cholesterol (30% mol/mol) tended to decrease the membrane interaction of maculatin 1.1. Anionic lipids locked maculatin 1.1 via electrostatic interactions onto the surface of oriented bilayers as seen in OCD spectra. Furthermore, increasing the membrane curvature by reducing the vesicle radii only slightly reduced the proportion of helical structure in all systems by approximately 10%. The peptide-lipid interaction was strongly dependent on both the lipid chain length and head group, which highlights the importance of the lipid composition used to mimic different cell types. This article is part of a Special Issue entitled: Membrane protein structure and function.
authors.affiliation: School of Chemistry, Bio21 Institute, University of Melbourne, VIC 3010, Australia. msani@unimelb.edu.au
authors.forename: Marc-Antoine
authors.initials: MA
authors.lastname: Sani
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Whitwell
authors.forename: Frances
authors.initials: F
authors.lastname: Separovic
mesh_terms: D029845:Amphibian Proteins / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D011898:Ranidae
chemical_list: D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D008051:Lipid Bilayers; C113499:maculatin-1.1 protein, Litoria