title: Spatial and temporal transcriptomic analysis of the Arabidopsis thaliana-Botrytis cinerea interaction.
abstract: Plants activate an array of defence responses following recognition of pathogenic organisms. This study attempted to characterize at a transcriptional level, the defence responses of Arabidopsis thaliana after infection with Botrytis cinerea using microarrays. Alteration in transcript levels following infection was investigated in time (temporal) and space (spatial). A number of genes were up- and down-regulated specifically at 12 h, others at 24 h while others were up- and down-regulated at both time points. Similarly, some genes were specifically induced very close to the lesion while others in more distal tissue. Clustering of expression profiles resulting from other biotic and abiotic interactions with Arabidopsis indicated a large overlap in gene expression. This study highlighted a multitude of genes induced in Arabidopsis spatially and temporally following infection with B. cinerea providing an insight into key processes of defence against this pathogen. The plethora of altered genes identified are candidates for further investigation.
authors.affiliation: Department of Molecular and Cell Biology, University of Cape Town, Private Bag 7701, Rondebosch, South Africa.
authors.forename: Joseph M K
authors.initials: JM
authors.lastname: Mulema
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Denby
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology*; D001483:Base Sequence; D020171:Botrytis / Q000502:physiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics; D059372:Nucleotide Motifs / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology; D011401:Promoter Regions, Genetic / Q000235:genetics; D015203:Reproducibility of Results; D013997:Time Factors; D015854:Up-Regulation / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA