title: Direct spectrophotometric assay for benzaldehyde lyase activity.
abstract: Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2'-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.
authors.affiliation: ITMC, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany.
authors.forename: Dessy
authors.initials: D
authors.lastname: Natalia
authors.forename: Christina
authors.initials: C
authors.lastname: Kohlmann
authors.forename: Marion B
authors.initials: MB
authors.lastname: Ansorge-Schumacher
authors.forename: Lasse
authors.initials: L
authors.lastname: Greiner