title: Differential proteomic analysis of highly purified placental cytotrophoblasts in pre-eclampsia demonstrates a state of increased oxidative stress and reduced cytotrophoblast antioxidant defense.
abstract: Proteomics were performed using highly (99.99%) purified cytotrophoblasts from six normal and six pre-eclamptic placentas. Eleven proteins were found which decreased in pre-eclampsia (actin, glutathione S-transferase, peroxiredoxin 6, aldose reductase, heat shock protein 60 (Hsp60), two molecular forms of heat shock protein 70 (Hsp70) β-tubulin, subunit proteasome, ezrin, protein disulfide isomerase, and phosphoglycerate mutase 1). Only one protein, α-2-HS-glycoprotein (fetuin), was found to increase its expression. Western blots of actin, Hsp70, ezrin, and glutatione S-transferase confirmed decrease in protein expression. Many of the proteins that decreased are consistent with a state of oxidative stress in the pre-eclamptic placenta and a decreased cytotrophoblast defense against and response to oxidative stress.
authors.affiliation: Maternal and Fetal Health Research Centre, University of Manchester, Manchester, UK.
authors.forename: Edward D
authors.initials: ED
authors.lastname: Johnstone
authors.forename: Grzgorz
authors.initials: G
authors.lastname: Sawicki
authors.forename: Larry
authors.initials: L
authors.lastname: Guilbert
authors.forename: Bonnie
authors.initials: B
authors.lastname: Winkler-Lowen
authors.forename: Virgilio J J
authors.initials: VJ
authors.lastname: Cadete
authors.forename: Donald W
authors.initials: DW
authors.lastname: Morrish
mesh_terms: D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D060748:Fetuins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics; D014327:Trophoblasts / Q000737:chemistry / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D060748:Fetuins