title: Improvement of transformation efficiency by strategic circumvention of restriction barriers in Streptomyces griseus.
abstract: DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-GC5mCGGC-3' and 5'-GAG5mCTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.
authors.affiliation: Chemical Biology Department, Advanced Science Institute, RIKEN, Saitama 351-0198, Japan. hirokap@xpost.plala.or.jp
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Shunji
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yoshida
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019175:DNA Methylation; D015280:DNA Restriction-Modification Enzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology* / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D015280:DNA Restriction-Modification Enzymes; D004269:DNA, Bacterial