title: [Effects of organic solvents on proliferation of Hela cells line].
abstract: OBJECTIVE
  To research the effects of organic solvents on proliferation inhibition of Hela cells line.
  
  
  METHODS
  The apoptosis of Hela cells induced by ethanol and dimethylsulfoxide (DMSO) were studied to analyse the error analyse from background organic solvents. The apoptosis of Hela cells induced by ethanol, DMSO and the combination of these two organic solvents with different concentrations were observed by MTT test and Giemsa staining.
  
  
  RESULTS
  The Hela cells proliferation were significantly inhibited by ethanol with the concentrations ranged from 30% to 100%, and DMSO with the concentrations ranged from 50% to 100% (P < 0.01). There were no significantly difference between the two combination of ethanol 40% + DMSO 60%, ethanol 30% + DMSO 70% and control group (P > 0. 05) respectively.
  
  
  CONCLUSIONS
  The cells proliferation were inhibited by organic solvents itself and were in a time and dose dependent. The background experimental error can be reduced remarkably by choosing the two organic solvents combination of ethanol and DMSO.
authors.affiliation: College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China. qyuz@163.com
authors.forename: Yu-zhi
authors.initials: YZ
authors.lastname: Qin
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Zhong
authors.forename: Gong-xi
authors.initials: GX
authors.lastname: Chen
authors.forename: De-wu
authors.initials: DW
authors.lastname: Zhao
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Yang
authors.forename: Xing-yao
authors.initials: XY
authors.lastname: Xiong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D004144:Diosgenin / Q000031:analogs & derivatives / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D012995:Solubility; D012997:Solvents / Q000494:pharmacology / Q000633:toxicity*; D013194:Staining and Labeling
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents; C019357:dioscin; D000431:Ethanol; D004144:Diosgenin; D004121:Dimethyl Sulfoxide