title: [Determination of blood/serum ratios of different forensically relevant analytes in authentic samples].
abstract: For forensic toxicological investigations only whole blood, but no serum is often available. Pharmacokinetic data are helpful for interpreting the results, but most of these studies indicate serum or plasma concentrations. In order to obtain reliable conversion factors which also take intersubject variability into account, the blood/serum ratios (B/S) of oxycodone, morphine, fentanyl, hydromorphone, zopiclone, MDMA, dexamphetamine, alprazolam, risperidone and 9-hydroxyrisperidone were determined by LC-MS/MS using authentic samples. Blood and corresponding serum samples were obtained from driving studies performed with controlled or known dosages of the above drugs. The analytes were analysed in blood and serum and the following mean B/S ratios (relative standard deviations) were determined: oxycodone 1.48 (8.19 %); morphine 1.03 (3.59 %); fentanyl 0.87 (13.9 %); hydromorphone 1.04 (8.11 %); zopiclone 0.89 (16.1 %); MDMA 1.19 (8.04 %); dexamphetamine 0.89 (10.9 %); alprazolam 0.81 (5.84 %); risperidone 0.65 (7.52 %); 9-hydroxyrisperidone 0.73 (12.3 %). These mean values are largely in line with those reported in the literature. The B/S ratios did not appear to depend on partition coefficients, whereas there was strong evidence that B/S ratios decreased with increasing plasma protein binding.
authors.affiliation: Institut für Rechtsmedizin und Verkehrsmedizin der Universität Heidelberg.
authors.forename: Ricarda
authors.initials: R
authors.lastname: Jantos
authors.forename: Markus
authors.initials: M
authors.lastname: Schuhmacher
authors.forename: Janet L
authors.initials: JL
authors.lastname: Veldstra
authors.forename: Wendy M
authors.initials: WM
authors.lastname: Bosker
authors.forename: Ineke
authors.initials: I
authors.lastname: Klöpping-Ketelaars
authors.forename: Katerina
authors.initials: K
authors.lastname: Touliou
authors.forename: Gian Marco
authors.initials: GM
authors.lastname: Sardi
authors.forename: Karel A
authors.initials: KA
authors.lastname: Brookhuis
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Ramaekers
authors.forename: Rainer
authors.initials: R
authors.lastname: Mattern
authors.forename: Gisela
authors.initials: G
authors.lastname: Skopp
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011619:Psychotropic Drugs / Q000097:blood*; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D015262:Xenobiotics / Q000097:blood*
chemical_list: D011619:Psychotropic Drugs; D015262:Xenobiotics