title: An optimized isotopic labelling strategy of isoleucine-γ2 methyl groups for solution NMR studies of high molecular weight proteins.
abstract: An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-γ(2) groups in proteins. The (2)H, (13)C-pattern of the biosynthetic precursor has been designed to optimize magnetization transfer, in large proteins, between these important structural probes and their corresponding backbone nuclei.
authors.affiliation: CNRS, Institut de Biologie Structurale, Grenoble, France.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ayala
authors.forename: Olivier
authors.initials: O
authors.lastname: Hamelin
authors.forename: Carlos
authors.initials: C
authors.lastname: Amero
authors.forename: Ombeline
authors.initials: O
authors.lastname: Pessey
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Plevin
authors.forename: Pierre
authors.initials: P
authors.lastname: Gans
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boisbouvier
mesh_terms: D007532:Isoleucine / Q000031:analogs & derivatives / Q000737:chemistry*; D007553:Isotope Labeling; D008958:Models, Molecular; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D011506:Proteins / Q000737:chemistry*; D012996:Solutions
chemical_list: D011506:Proteins; D012996:Solutions; D007532:Isoleucine