title: The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii shows enhanced cold tolerance.
abstract: Five intergeneric hybrids between the chrysanthemum cultivar 'Zhongshanjingui' (as female) and Ajania przewalskii (as male) were obtained with the help of embryo culture. While 'Zhongshanjingui' bears a standard anemone type flower and A. przewalskii a non-anemone type one, the inflorescence type of the hybrids varied. The diameter of the hybrids' flowers was intermediate between those of the parents. The chromosome number of the hybrids was 2n = 45, of which GISH analysis was able to establish that 27 were inherited from 'Zhongshanjingui' and the other 18 from A. przewalskii. A combination of various assays was used to show that the cold tolerance of the hybrids was equivalent to that of the highly tolerant A. przewalskii parent. Enhanced cold tolerance was correlated with an increase in free proline and a decrease in malondialdehyde content.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Deng
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.forename: Xi
authors.initials: X
authors.lastname: Cheng
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
mesh_terms: D019659:Asteraceae / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D032461:Chromosomes, Plant; D031202:Chrysanthemum / Q000196:embryology / Q000235:genetics / Q000502:physiology*; D003080:Cold Temperature; D006824:Hybridization, Genetic; D008315:Malondialdehyde / Q000032:analysis; D011392:Proline / Q000032:analysis; D013312:Stress, Physiological
chemical_list: D008315:Malondialdehyde; D011392:Proline