title: [Neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons of newborn rat].
abstract: OBJECTIVE
  To study the neurotrophic effects of protocatechuic acid on neurite outgrowth and survival in cultured cerebral cortical neurons.
  
  
  METHODS
  The newborn rat cerebral cortex neurons were cultured in vitro. The convert phase microscope was used to count the survival neurons with neurites and measure the average length of neurites. MTT and LDH assay were carried out to investigate the effect of PCA on the neuronal viability.
  
  
  RESULTS
  Compared with control group, different concentration of PCA could increase the number of survival neurons with neurites and the average length of neurites. MTT and LDH assay showed that PCA promoted neuron survival in a dose-dependent manner.
  
  
  CONCLUSIONS
  PCA can enhance the survival of rat cortical neurons with neurites and promote the neurite outgrowth of rat cortical neurons.
authors.affiliation: School of Medicine, Jinan University, Guangzhou 510632, China. xuexiaoyan816@sina.com
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Xue
authors.forename: Lian-feng
authors.initials: LF
authors.lastname: Lin
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
authors.forename: Ting
authors.initials: T
authors.lastname: Pi
authors.forename: Yong-chang
authors.initials: YC
authors.lastname: Lai
authors.forename: Huan-min
authors.initials: HM
authors.lastname: Luo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D004305:Dose-Response Relationship, Drug; D005260:Female; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000494:pharmacology*; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008297:Male; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D062385:Hydroxybenzoates; C009091:protocatechuic acid; D045022:Lactate Dehydrogenases