title: Sex identification using the ZFX and ZFY genes in common marmosets (Callithrix jacchus).
abstract: We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483-bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY showed the recognition sites of two restriction enzymes, DdeI and MseI, respectively. After digestion of the products using each enzyme, we found that the band patterns between females and males were different. PCR-based sex identification might provide a tool for further breeding studies and experimental embryological studies using marmosets.
authors.affiliation: Experimental Animals Institute, Hamamatsu University School of Medicine. 1–20–1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Takabayashi
authors.forename: Hideki
authors.initials: H
authors.lastname: Katoh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002144:Callithrix / Q000235:genetics*; D004247:DNA / Q000032:analysis; D005260:Female; D024282:Genes, sry / Q000235:genetics; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012732:Sex Determination Analysis / Q000379:methods*; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D051741:Kruppel-Like Transcription Factors; C062076:zinc finger protein, X-linked; D004247:DNA