title: Mesenchymal stem cell-based tissue engineering of small-diameter blood vessels.
abstract: The aim of the study was to construct small-diameter vascular grafts using canine mesenchymal stem cells (cMSCs) and a pulsatile flow bioreactor. cMSCs were isolated from canine bone marrow and expanded ex vivo. cMSCs were then seeded onto the luminal surface of decellularized arterial matrices, which were further cultured in a pulsatile flow bioreactor for four days. Immunohistochemical staining and scanning electron microscopy was performed to characterize the tissue-engineered blood vessels. cMSCs were successfully seeded onto the luminal surface of porcine decellularized matrices. After four-day culture in the pulsatile flow bioreactor, the cells were highly elongated and oriented to the flow direction. Immunohistochemistry demonstrated that the cells cultured under pulsatile flow expressed Von Willebrand factor, an endothelial cell marker. In conclusion, cMSCs seeded onto decellularized arterial matrices could differentiate into endothelial lineage after culturing in a pulsatile flow bioreactor, which provides a novel approach for tissue engineering of small-diameter blood vessels.
authors.affiliation: Department of Cardiothoracic Surgery, Beijing Electric Power Hospital, Beijing, People's Republic of China.
authors.forename: Jian-De
authors.initials: JD
authors.lastname: Dong
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019149:Bioreactors; D001808:Blood Vessels / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004285:Dogs; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D011673:Pulsatile Flow; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D015415:Biomarkers; D014841:von Willebrand Factor