title: Neurotoxic effect of maneb in rats as studied by neurochemical and immunohistochemical parameters.
abstract: Epidemiological investigations document that workers in agriculture, horticulture and people living near areas with frequent use of pesticides have increased risk of developing symptoms of Parkinson's disease. This study investigated the neurotoxic effect of the fungicide maneb by morphological, immunohistochemical and neurochemical methods applying young Sprague-Dawley male rat as the model. Intraperitoneal dosing (7.5, 15 or 30mg maneb/kg bodyweight/week for 12 weeks) demonstrated dose-related increased manganese concentration in corpus striatum. The striatal concentration of 5-hydroxytryptamine (5-HT) increased in a dose-related manner, as did the 5-HT concentrations in the rest of the brain indicating early sign of neurotoxicity. Striatal acetylcholinesterase activity was not affected. The concentrations of noradrenaline, dopamine, neurotransmitter amino acids and the levels of the proteins α-synuclein and synaptophysin in corpus striatum and the rest of the brain were not changed. No histological parameter was affected when studied in corpus striatum and substantia nigra.
authors.affiliation: The Danish Institute for Food and Veterinary Research (DFVF), Department of Toxicology and Risk Assessment, DK-2860 Søborg, Denmark.
authors.forename: Brian Svend
authors.initials: BS
authors.lastname: Nielsen
authors.forename: Erik H
authors.initials: EH
authors.lastname: Larsen
authors.forename: Ole
authors.initials: O
authors.lastname: Ladefoged
authors.forename: Henrik Rye
authors.initials: HR
authors.lastname: Lam