title: Shear stress magnitude is critical in regulating the differentiation of mesenchymal stem cells even with endothelial growth medium.
abstract: Human mesenchymal stem cells (MSC) were seeded onto the inner surface of a tubular silicon construct and, after 24 h, were exposed to a shearing stress of either 2.5 or 10 dyne/cm(2) for 1 day. The fluid contained endothelial growth factors in both cases. Morphological changes and cytoskeletal rearrangements were observed in the stimulated cells. Immunofluorescence staining showed that low (2.5 dyne/cm(2)) and high shear stress (10 dyne/cm(2)) resulted in the expression of von Willebrand factor (vWF) and calponin, respectively. At low shear stress, CD31 (PECAM-1) was significantly expressed whereas vWF and KDR expression was only slightly higher than those under 10 dyne/cm(2). All three markers related to smooth muscle cells (myocardin, myosin heavy chain, and SM-22α) had significantly higher expression under shear stress of 10 dyne/cm(2) compared with a 2.5 dyne/cm(2), even in endothelial growth medium. Shear stress plays a critical role in regulating MSC differentiation and must be considered for bioengineered blood vessels.
authors.affiliation: Department of Biomedical Engineering, Inje University, Rm #309, BLDG-A, 607 Obang-Dong, Gimhae, Gyeongnam 621-749, Republic of Korea.
authors.forename: Dong Hwa
authors.initials: DH
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Su-Hyang
authors.initials: SH
authors.lastname: Kim
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Shin
authors.forename: So Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Jung-Woog
authors.initials: JW
authors.lastname: Shin
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000187:drug effects / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D033081:Shear Strength / Q000187:drug effects
chemical_list: D016228:Endothelial Growth Factors