title: Circulating interleukin-27 levels in Helicobacter pylori-infected patients with gastric or duodenal ulcers, independent of the bacterial cytotoxin-associated gene A virulence factor.
abstract: OBJECTIVE
  The aim was to evaluate the interleukin (IL)-27 levels in Helicobacter pylori (H. pylori)-infected patients with gastric ulcer (GU) or duodenal ulcer (DU) and to determine its association with H. pylori virulence factor cytotoxin-associated gene A (CagA).
  
  
  METHODS
  In all, 127 H. pylori infected patients (including 96 DU patients, of whom 61 were anti-CagA(+) and 35 were anti-CagA(-)) and 31 GU patients (of whom 15 were anti-CagA(+) and 16 were anti-CagA(-)), 60 asymptomatic (AS) carriers (of whom 30 were anti-CagA(+) and 30 were anti-CagA(-)) and 30 healthy H. pylori-negative participants (as a control) were enrolled in the study. Serum concentrations of IL-27 were measured by the enzyme-linked immunosorbent assay method.
  
  
  RESULTS
  The mean levels of IL-27 in the GU (44.26 ± 7.12 pg/mL) and DU patients (40.84 ± 3.90 pg/mL) was significantly higher than those observed in the AS carriers (22.06 ± 1.90 pg/mL, P < 0.001) and the control group (18.12 ± 1.68 pg/mL, P < 0.001 and P < 0.002, respectively). In the GU, DU and AS groups the levels of IL-27 in anti-CagA(+) participants were not significantly differ from that in the anti-CagA(-) participants.
  
  
  CONCLUSIONS
  These results showed that the mean concentration of IL-27 in H. pylori-infected peptic ulcer (PU) patients was higher than in AS carriers and the healthy control group. The serum concentrations of IL-27 were not affected by the CagA factor.
authors.affiliation: Department of Microbiology and Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. jafarzadeh14@yahoo.com
authors.forename: Abdollah
authors.initials: A
authors.lastname: Jafarzadeh
authors.forename: Maryam
authors.initials: M
authors.lastname: Nemati
authors.forename: Mohammad-Taghi
authors.initials: MT
authors.lastname: Rezayati
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000097:blood*; D058070:Asymptomatic Diseases; D001426:Bacterial Proteins / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004381:Duodenal Ulcer / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood* / Q000150:complications* / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007378:Interleukins / Q000097:blood* / Q000276:immunology; D008875:Middle Aged; D013276:Stomach Ulcer / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D037521:Virulence Factors / Q000097:blood
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015415:Biomarkers; D007378:Interleukins; C464565:MYDGF protein, human; D037521:Virulence Factors; C080866:cagA protein, Helicobacter pylori