title: [Study on the extraction of total alkaloid from folium nelumbinis with ultrasonic wave technology optimized by central composite design and response surface method].
abstract: OBJECTIVE
  To optimize the extracting process of Folium Nelumbinis by central composite design and response surface method.
  
  
  METHODS
  Independent variables were ethanol concentration, solvents ratio and pH,dependent variable was extracting rate of alkaloid. Composite design and response surface method were used to optimize the extracting process. Resultant model was used to predict the response in the optimal region.
  
  
  RESULTS
  The optimum extraction conditions were 80% aqueous ethanol, 75 fold solvent, pH value 5.
  
  
  CONCLUSIONS
  The optimum process is simple and more convenient for extracting Folium Nelumbinis. The observed and predicted values are close to each other.
authors.affiliation: College of pharmacy, Taishan Medical University, Taian 271016, China. wfg713@sina.com
authors.forename: Fu-gang
authors.initials: FG
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yu-xia
authors.initials: YX
authors.lastname: Wang
authors.forename: Yun-sheng
authors.initials: YS
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D030721:Nymphaea / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods; D014465:Ultrasonics
chemical_list: D000470:Alkaloids; D012997:Solvents; D000431:Ethanol