title: Luteinising hormone β-subunit gene Gly102Ser variant and oxidative stress biomarkers in Egyptian infertile males.
abstract: The aim of the present study was to evaluate the association of Glycine102Serine molecular variant of luteinising hormone (LH) β-subunit gene with infertility in Egyptian males and to assess the impact of oxidative stress in association with Glycine102Serine molecular variant on male infertility. Fifty men with idiopathic oligozoospermia were enrolled in the study. The control group consisted of 39 men with normal semen parameters and proven fertility. Variable oxidative status markers were evaluated. Glycine102Serine variant was investigated using restriction fragment length polymorphism-polymerase chain reaction. The study concluded that Glycine102Serine variant of LH β-subunit gene is relatively common among infertile Egyptian males and might be implicated in some Egyptian infertility cases. In addition, the association of this variant, in some cases, with disturbed oxidative status (decreased seminal plasma antioxidants' level and increased sperm lipid peroxidation) probably have a significant role in accentuation of infertility in those particular cases.
authors.affiliation: Faculty of Medicine, Department of Clinical Pathololgy, Alexandria University, Alexandria, Egypt. doaa_hashad2003@yahoo.com
authors.forename: D
authors.initials: D
authors.lastname: Hashad
authors.forename: N
authors.initials: N
authors.lastname: Mohamed
authors.forename: M M
authors.initials: MM
authors.lastname: Hashad
mesh_terms: D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D017931:DNA Primers; D004534:Egypt; D005998:Glycine / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism*; D037101:Luteinizing Hormone, beta Subunit / Q000235:genetics*; D008297:Male; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012694:Serine / Q000235:genetics*
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D037101:Luteinizing Hormone, beta Subunit; D012694:Serine; D005998:Glycine