title: [Detection and molecular characterization of human parechovirus (HPeV) in children with acute gastroenteritis].
abstract: OBJECTIVE
  To study HPeV from stool samples of children with acute gastroenteritis under 5 years old.
  
  
  METHODS
  We conducted a real-time PCR to detect HPeV from stool samples and to amply VP1 sequence by nested RT-PCR to identify HPeV type.
  
  
  RESULTS
  The results showed that 27 of 306 (8.82%) children with acute gastroenteritis were infected HPeV. 11 strains were typed. 9 strains HPeV1, both HPeV2 and HPeV4 was 1 strain. HPeV was mostly identified in autumn season with a peak in July. HPeV seemed relevant in children >2 years old. The range of nucleotide identity between all isolated strains with reference strains was 79%-92%.
  
  
  CONCLUSIONS
  Epidemiology characteristic of HPeV in Jilin was concordance with that of reports. HPeV3 wasnt detected. It's significant to conduct the large scale and long-term surveillance of HPeV.
authors.affiliation: College of Public Health, Harbin Medical University, Harbin 150086, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zi-qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Wei-xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Dan-di
authors.initials: DD
authors.lastname: Li
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Li
authors.forename: Jin-xin
authors.initials: JX
authors.lastname: Xie
authors.forename: Shu-xian
authors.initials: SX
authors.lastname: Cui
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Jing-bo
authors.initials: JB
authors.lastname: Zhao
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000208:Acute Disease; D002675:Child, Preschool; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D007223:Infant; D008297:Male; D030061:Parechovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny