title: [Detection and clinical study on coronavirus HKU1 with acute lower respiratory tract infections of hospitalized children in Changsha].
abstract: OBJECTIVE
  In order to understand the epidemiological and virologic characteristics of coronavirus HKU1 infection in hospitalized children with acute respiratory tract infection (ARTI) in Changsha.
  
  
  METHODS
  1165 nasopharyngeal aspirates (NPA) specimens were collected from hospitalized children with ARTI between September 2007 and August 2008 in Changsha. Specimens were screened for pol gene of coronavirus HKU1 by polymerase chain reaction. All positive amplification products were confirmed by sequencing and compared with those in GenBank.
  
  
  RESULTS
  Coronavirus HKU1 were detected in 12 patients (1.03%) out of the 1165 children. The patients were from 8 days to 3 years. The most common clinical diagnosis was bronchopneumonia(83.33%). Similarity of coronavirus HKU1 with those published in the GenBank at nucleotide levels was 98.18% - 100%.
  
  
  CONCLUSIONS
  Coronavirus HKU1 may be important pathogens in children with acute lower respiratory tract infection. Coronavirus HKU1 infections are common in children under 3 years old. There is no significant difference in the infectious rate between the boys and the girls. The peak of its prevalence is in spring and winter. A single genetic lineage of Coronavirus HKU1 was revealed in human subjects in Changsha.
authors.affiliation: University of South China, Hengyang 421001, China.
authors.forename: Ni-guang
authors.initials: NG
authors.lastname: Xiao
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Qiong-hua
authors.initials: QH
authors.lastname: Zhou
authors.forename: Rong-fang
authors.initials: RF
authors.lastname: Zhang
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhong
authors.forename: Han-chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Ding
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Jing-rong
authors.initials: JR
authors.lastname: Song
authors.forename: Yun-de
authors.initials: YD
authors.lastname: Hou
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000208:Acute Disease; D002673:Child, Hospitalized; D002675:Child, Preschool; D002681:China; D017934:Coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000821:virology*