title: Purification and characterization of an endoxylanase from the culture broth of Bacillus cereus BSA1.
abstract: An extracellular xylanase from the fermented broth of Bacillus cereus BSA1 was purified and characterized. The enzyme was purified to 3.43 fold through ammonium sulphate precipitation, DEAE-cellulose chromatography and followed by gel filtration through Sephadex G-100 column. The molecular mass of the purified xylanse was about 33 kDa. The enzyme was an endoxylanase as it initially degraded xylan to xylooligomers. The purified enzyme showed optimum activity at 55 degrees C and at pH 7.0 and remained reasonably stable in a wide range ofpH (5.0-8.0) and temperature (40-65 degrees C). The Km and Vmax values were found to be 8.2 mg/ml and 181.8 micromol/(min mg), respectively. The enzyme had no apparent requirement ofcofactors, and its activity was strongly inhibited by Cu++, Hg++. It was also a salt tolerant enzyme and stable upto 2.5 M of NaCl and retained its 85% activity at 3.0 M. For stability and substrate binding, the enzyme needed hydrophobic interaction that revealed when most surfactants inhihited xylanase activity. Since the enzyme was active over wide range ofpH, temperature and remained active in higher salt concentration, it could find potential uses in biobleaching process in paper industries.
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapur-721102, West Bengal, India.
authors.forename: A
authors.initials: A
authors.lastname: Mandal
authors.forename: S
authors.initials: S
authors.lastname: Kar
authors.forename: P K
authors.initials: PK
authors.lastname: Das Mohapatra
authors.forename: C
authors.initials: C
authors.lastname: Maity
authors.forename: B R
authors.initials: BR
authors.lastname: Pati
authors.forename: K C
authors.initials: KC
authors.lastname: Mondal
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D001409:Bacillus cereus / Q000737:chemistry / Q000201:enzymology*; D001709:Biotechnology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D045604:Extracellular Fluid / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D007700:Kinetics; D019216:Metals, Heavy / Q000494:pharmacology; D008970:Molecular Weight; D012492:Salts / Q000494:pharmacology; D013696:Temperature; D014990:Xylans / Q000378:metabolism*
chemical_list: D019216:Metals, Heavy; D012492:Salts; D014990:Xylans; D043364:Endo-1,4-beta Xylanases; D000645:Ammonium Sulfate