title: A reference map of the membrane proteome of Enterococcus faecalis.
abstract: Enterococcus faecalis is a gram-positive bacterium that is part of the indigenous microbiotica of humans and animals as well as an opportunistic pathogen. In this study, we have fractionated the membrane proteome of E. faecalis and identified many of its constituents by mass spectrometry. We present blue native-/SDS-PAGE reference maps that contain 102 proteins. These proteins are important for cellular homeostasis, virulence, and antibiotic intervention. Intriguingly, many proteins with no known function were also identified, indicating that there are substantial gaps in the knowledge of this organism's biology. On a more limited scale, we also provide insight into the composition of membrane protein complexes. This study is a first step toward elucidating the membrane proteome of E. faecalis, which is critical for a better understanding of how this bacterium interacts with a host and with the extracellular milieu.
authors.affiliation: Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Maddalo
authors.forename: Peter
authors.initials: P
authors.lastname: Chovanec
authors.forename: Filippa
authors.initials: F
authors.lastname: Stenberg-Bruzell
authors.forename: Hailyn V
authors.initials: HV
authors.lastname: Nielsen
authors.forename: Michael I
authors.initials: MI
authors.lastname: Jensen-Seaman
authors.forename: Leopold L
authors.initials: LL
authors.lastname: Ilag
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Kline
authors.forename: Daniel O
authors.initials: DO
authors.lastname: Daley
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D013293:Enterococcus faecalis / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001426:Bacterial Proteins; D020543:Proteome