title: [Optimization of cultivation conditions of alpha-L-rhamnosidases producers-- representatives of different taxonomic groups of microorganisms].
abstract: Influence ofsome technological parameters of cultivation ofproducers Cryptococcus albidus, Eupenicillinum erubescens, Bacillus sp. on the process of synthesis of extracellular enzyme alpha-L-rhamnosidase has been studied. The authors have determined optimal sources of carbon (0.2-0.3% rhamnose) and nitrogen (0.2% sodium nitrate for C. albidus and E. erubescens and ammonium sulphate for Bacillus sp.) (the ratio 1:2), cultivation temperature (28 degrees C, 25 degrees C, 42 degrees C, respectively) for maximum synthesis of alpha-L-rhamnosidase. Use of the medium with initial pH value from 4 to 8 was most efficient for all the studied strains. The maximum level of alpha-L-rhamnosidase activity of E. erubescens and Bacillus sp. was established at the value of sulphite number of 0.44, while for C. albidus--it was 0.56. Maximum alpha-L-rhamnosidase activity of C. albidus, E. erubescens, Bacillus sp. is achieved at 4, 8 days and 27 hours of cultivation, respectively. The cultures being grown in selected conditions, the alpha-L-rhamnosidase synthesis has increased by 30, 50 and 20%, respectively.
authors.forename: E V
authors.initials: EV
authors.lastname: Hudzenko
authors.forename: N V
authors.initials: NV
authors.lastname: Borzova
authors.forename: L D
authors.initials: LD
authors.lastname: Varbanets'
mesh_terms: D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D001431:Bacteriological Techniques / Q000379:methods; D003454:Cryptococcus / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D055324:Eupenicillium / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D009172:Mycology / Q000379:methods; D013696:Temperature
chemical_list: D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase