title: Crystallographic characterization of the DIX domain of the Wnt signalling positive regulator Ccd1.
abstract: Coiled-coil DIX1 (Ccd1) is a positive regulator that activates the canonical Wnt signalling pathway by inhibiting the degradation of the key signal transducer β-catenin. The C-terminal DIX domain of Ccd1 plays an important role in the regulation of signal transduction through homo-oligomerization and protein complex formation with other DIX domain-containing proteins, i.e. axin and dishevelled proteins. Here, the expression, purification, crystallization and X-ray data collection of the Ccd1 DIX domain are reported. The crystals of the Ccd1 DIX domain belonged to space group P2(1)2(1)2(1), with unit-cell parameters a=72.9, b=75.7, c=125.6 Å. An X-ray diffraction data set was collected at 3.0 Å resolution.
authors.affiliation: Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Terawaki
authors.forename: Koumei
authors.initials: K
authors.lastname: Yano
authors.forename: Takuya
authors.initials: T
authors.lastname: Katsutani
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shiomi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Keino-Masu
authors.forename: Masayuki
authors.initials: M
authors.lastname: Masu
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shomura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Komori
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism
chemical_list: C509983:Dixdc1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D051153:Wnt Proteins