title: Identification of classifiers for increase or decrease of thyroid peroxidase activity in the FTC-238/hTPO recombinant cell line.
abstract: Thyroid peroxidase (TPO) plays an important role in thyroid hormone biosynthesis, as it catalyzes all of the essential steps in iodide organification. TPO activity can be detected using the guaiacol assay; however, this assay is complex and very time-consuming. Therefore, we focused on devising a simplified method using microarrays to detect changes in TPO activity, which is a target for disruption of the thyroid hormone axis. These experiments have systematically assessed the potential utility of transcriptomic end points as enhancements to the guaiacol assay. Previously, we demonstrated that benzophenone-2, benzophenone, perfluorooctane sulfonate, bisphenol A bis ether, and vinclozolin decreased TPO activity, and that dibutyl phthalate, carbaryl, dibenzo(a,h)anthracene, benzo(a)pyrene, and methylmercury increased TPO activity. In this work, we used human oligonucleotide chips to examine changes in the gene expression profile of FTC-238 human follicular thyroid carcinoma cells expressing human recombinant TPO, after exposure of the cells to TPO activity-disrupting agents. We identified 362 classifiers that could predict the effect of the toxicants on TPO activity with about 70% accuracy. These classifiers are potential markers for predicting the effects of chemicals on thyroid hormone production.
authors.affiliation: Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and Technology (KIST), Republic of Korea.
authors.forename: Mee
authors.initials: M
authors.lastname: Song
authors.forename: Youn-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Song
authors.forename: Han-Seam
authors.initials: HS
authors.lastname: Choi
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Park
authors.forename: Jae-Chun
authors.initials: JC
authors.lastname: Ryu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D014162:Transfection
chemical_list: D004785:Environmental Pollutants; D007453:Iodide Peroxidase