title: [Prokaryotic expression and purification of human histone-1 and its activity detection].
abstract: OBJECTIVE
  To construct the prokaryotic expression vector of human histone-1, obtain the purified GST-H1 protein, and detect its activity.
  
  
  METHODS
  Human histone-1 coding region was amplified from human mammary cDNA library, and was inserted into prokaryotic expression vector pGEX-KG. The recombinant plasmid pGEX-KG-H1 was transformed into E.coli Rossate. The expressed product was purified by GST-Sepharose 4B beads and identified by SDS-PAGE and Western blot analysis.
  
  
  RESULTS
  The DNA fragment of about 650 bp was successfully amplified by PCR, cloned into pGEX-KG, and identified by sequencing. The recombinant protein of about M(r); 52 000 was successfully induced, purified and tested well by Kinase assay.
  
  
  CONCLUSIONS
  The recombinant protein of GST-H1 is obtained successfully, which lay the foundation for further research on cell cycle control.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100850, China.
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Xu
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Fan
authors.forename: Ling-xue
authors.initials: LX
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Ding
authors.forename: Nan
authors.initials: N
authors.lastname: DU
authors.forename: Qi-nong
authors.initials: QN
authors.lastname: Ye
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors / Q000235:genetics; D006657:Histones / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D006657:Histones; D011993:Recombinant Fusion Proteins