title: Quantification of bovine milk oligosaccharides using liquid chromatography-selected reaction monitoring-mass spectrometry.
abstract: Oligosaccharides are important components of milk with bioefficacy as prebiotics, anti-infectives, and immune system modulators and as a possible source of sialic acid for neural function. Bovine milk oligosaccharides are lower in concentration and lack the diversity of human milk oligosaccharides but could be a commercial source of milk oligosaccharides for pediatric foods. For this development, an ability to quantify the oligosaccharides is required. This study validated a hydrophilic interaction chromatography high-performance liquid chromatography-high-resolution selected reaction monitoring-mass spectrometry (HILIC HPLC-HRSRM-MS) method for measuring six different oligosaccharides in bovine milk, bovine colostrum, and infant formulas. The extraction resulted in a high recovery (90-103%) with a repeatability coefficient of variation ranging from 2 to 9% for the two dominant oligosaccharides, 3'-sialyllactose and 6'-sialyllactose, and ranging from 1 to 17% for the much lower concentration oligosaccharides, 6'-sialyllactosamine, disialyllactose, and N-acetylgalactosaminyllactose. The sixth oligosaccharide, 3'-sialyllactosamine, was not detected in any of the samples.
authors.affiliation: Fonterra Research Centre, Dairy Farm Road, Private Bag 11029, Palmerston North 4412, New Zealand. bertram.fong@fonterra.com
authors.forename: Bertram
authors.initials: B
authors.lastname: Fong
authors.forename: Kevin
authors.initials: K
authors.lastname: Ma
authors.forename: Paul
authors.initials: P
authors.lastname: McJarrow
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003126:Colostrum / Q000737:chemistry; D005260:Female; D006801:Humans; D007223:Infant; D041943:Infant Formula / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results
chemical_list: D009844:Oligosaccharides