title: Detection of elevated antibodies against SR protein kinase 1 in the serum of Alzheimer's disease patients.
abstract: Autoantibodies targeting specific cellular antigens are often present in sera and cerebrospinal fluids (CSFs) of patients with Alzheimer's disease (AD) and could play a role in the onset and/or progression of the disease. In this study we identified SR Protein Kinase 1 (SRPK1) as a new autoantigen elevated in AD. SRPK1, the prototype of the serine/arginine family of kinases, has been implicated in the regulation of multiple cellular processes such as pre-mRNA splicing, cell proliferation, chromatin structure, nuclear import and germ cell development. Using an ELISA assay, anti-SRPK1 antibodies, targeting mainly the first catalytic domain of the kinase, were detected in sera of patients with AD, at significantly elevated levels as compared to control subjects. The findings of this study document for the first time the existence of antibodies targeting SRPK1 in human sera and are indicative of a correlation between the levels of a-SRPK1 antibodies and the incidence of AD.
authors.affiliation: Laboratory of Biochemistry, Department of Chemistry, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
authors.forename: Makrina
authors.initials: M
authors.lastname: Daniilidou
authors.forename: Magda
authors.initials: M
authors.lastname: Tsolaki
authors.forename: Thomas
authors.initials: T
authors.lastname: Giannakouros
authors.forename: Eleni
authors.initials: E
authors.lastname: Nikolakaki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000134:cerebrospinal fluid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000134:cerebrospinal fluid; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000276:immunology*; D018709:Statistics, Nonparametric
chemical_list: D007074:Immunoglobulin G; C087728:SRPK1 protein, human; D017346:Protein Serine-Threonine Kinases