title: High-throughput functional marker assay for detection of Xa/xa and fgr genes in rice (Oryza sativa L.).
abstract: We apply CE for high-throughput analysis of functional markers for marker-assisted selection in rice. The accuracy, throughput and reproducibility of CE analysis for sequence-tagged site (STS) and simple sequence repeat (SSR) markers for bacterial blight resistance and aroma genes are demonstrated by using a CE system. Multiplex PCR products displayed well-differentiated allelic variants using different STS and SSR markers for identification of xa13, Xa21 and fgr genes using the CE system compared to 1.2% agarose gel images. Moreover, consumption of PCR product is much less in the CE system compared to traditional agarose gel systems. Sample consumption is less than 0.1 μL per analysis, thereby conserving samples for further downstream analysis. Out of 29 genotypes in BC(1)F(3) generation, 16 plants were found homozygous for all the three genes, viz., xa13, Xa21 and fgr. These homozygous lines can be used as potential donors in rice breeding programmes.
authors.affiliation: Plant Molecular Genetics, Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA.
authors.forename: Romesh K
authors.initials: RK
authors.lastname: Salgotra
authors.forename: Reginald J
authors.initials: RJ
authors.lastname: Millwood
authors.forename: Sujata
authors.initials: S
authors.lastname: Agarwal
authors.forename: C Neal
authors.initials: CN
authors.lastname: Stewart
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D017343:Genes, Plant; D005819:Genetic Markers; D057166:High-Throughput Screening Assays / Q000379:methods*; D018895:Microsatellite Repeats; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D020794:Receptor Protein-Tyrosine Kinases