title: Self-assembly of tissue transglutaminase into amyloid-like fibrils using physiological concentration of Ca2+.
abstract: Tissue transglutaminase (tTG or TG2) is a member of the transglutaminase family that catalyzes calcium dependent formation of isopeptide bonds. It has been shown that the expression of TG2 is elevated in neurodegenerative diseases such as Parkinson's, Huntington's, and Alzheimer's. We have investigated the self-assembly of TG2 in vitro. First, using software, hot spots, which are prone for aggregation, were identified in domain 2 of the enzyme. Next we expressed and purified recombinant TG2 and its truncated version that contains only the catalytic domain, and examined their amyloidogenic behavior in various conditions including different temperatures and pHs, in the presence of metal ions and Guanosine triphosphate (GTP). To analyze various stages leading to TG2 fibrillation, we employed various techniques including Thioflavin T (ThT) binding assay, Congo-Red, birefringence, Circular Dichroism (CD), 8-anilino-1-naphthalene sulfonic acid (ANS) binding, Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). Our results indicated that using low concentrations of Ca(2+), TG2 self-assembled into amyloid-like fibrils; this self-assembly occurred at the physiological temperature (37 °C) and at a higher temperature (57 °C). The truncated version of TG2 (domain 2) also forms amyloid-like fibrils only in the presence of Ca(2+). Because amyloid formation has occurred with domain 2 alone where no enzymatic activity was shown, self-cross-linking by the enzyme was ruled out as a mechanism of amyloid induction. The self-assembly of TG2 was not significant with magnesium and zinc ions, indicating specificity of the self-assembly for calcium ions. The calcium role in self-assembly of TG2 into amyloid may be extended to other proteins with similar biophysical properties to produce novel biomaterials.
authors.affiliation: Department of Molecular Medicine, School of Advanced Technology for Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran. hkalhor@gmail.com
authors.forename: Hamid R
authors.initials: HR
authors.lastname: Kalhor
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Shahin V
authors.forename: Mohamad H
authors.initials: MH
authors.lastname: Fouani
authors.forename: Hossein
authors.initials: H
authors.lastname: Hosseinkhani
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D019204:GTP-Binding Proteins; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D011503:Transglutaminases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000682:Amyloid; D011994:Recombinant Proteins; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins; D002118:Calcium