title: Live non-invasive Shigella dysenteriae 1 strain induces homologous protective immunity in a guinea pig colitis model.
abstract: A non-invasive live transconjugant Shigella hybrid (LTSHΔstx) strain was constructed from a Shiga toxin gene deleted mutant of Shigella dysenteriae 1 by introducing a plasmid vector pPR1347 that carried a lipopolysaccharide biosynthesis gene (rfb and rfc) of Salmonella typhimurium. In guinea pigs, four successive oral administrations of LTSH Δstx showed complete protection against rectal challenge with wild type S. dysenteriae 1 strain. Exponential increase of the serum IgG and IgA titer against lipopolysaccharide of LTSH Δstx was observed during immunization, peaked on day 28 and remained at that level until day 35 after the initiation of the immunization. In intestinal lavage of the immunized animals, significant increase of IgA titer against lipopolysaccharide of LTSH Δstx was also observed. These data suggested that LTSH Δstx could be a useful candidate to induce protective immunity against S. dysenteriae 1 infection.
authors.affiliation: National Institute of Cholera and Enteric Diseases, Kolkata, India.
authors.forename: Soumik
authors.initials: S
authors.lastname: Barman
authors.forename: Ranajit
authors.initials: R
authors.lastname: Kumar
authors.forename: Goutam
authors.initials: G
authors.lastname: Chowdhury
authors.forename: Dhira
authors.initials: D
authors.lastname: Rani Saha
authors.forename: Takeaki
authors.initials: T
authors.lastname: Wajima
authors.forename: Takashi
authors.initials: T
authors.lastname: Hamabata
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Gopinath
authors.initials: G
authors.lastname: Balakrish Nair
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Takeda
authors.forename: Hemanta
authors.initials: H
authors.lastname: Koley
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D004195:Disease Models, Animal; D004405:Dysentery, Bacillary / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D012762:Shigella dysenteriae / Q000276:immunology* / Q000502:physiology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008070:Lipopolysaccharides