title: Characterization of cellobiohydrolase from a newly isolated strain of Agaricus arvencis.
abstract: A highly efficient cellobiohydrolase (CBH)-secreting basidiomycetous fungus, Agaricus arvensis KMJ623, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular CBH was purified to homogeneity from A. arvencis culture supernatant using sequential chromatography. The relative molecular mass of A. arvencis CBH was determined to be 65 kDa by SDSPAGE and 130 kDa by size-exclusion chromatography, indicating that the enzyme is a dimer. A. arvencis CBH showed a catalytic efficiency (kcat/Km) of 31.8 mM⁻¹ s⁻¹ for p-nitrophenyl-beta-D-cellobioside, the highest level seen for CBH-producing microorganisms. Its internal amino acid sequences showed significant homology with CBHs from glycoside hydrolase family 7. Although CBHs have been purified and characterized from other sources, A. arvencis CBH is distinguished from other CBHs by its high catalytic efficiency.
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Korea.
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Dayanand
authors.initials: D
authors.lastname: Kalyani
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: In-Won
authors.initials: IW
authors.lastname: Kim
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Jeya
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000364:Agaricus / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D055503:Protein Multimerization; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D005960:Glucosides; C034170:4-nitrophenyl beta-cellobioside; D043366:Cellulose 1,4-beta-Cellobiosidase