title: Identifying the early post-HIV antibody seroconversion period.
abstract: BACKGROUND
  Identifying persons with recent human immunodeficiency virus (HIV) antibody seroconversion is useful for treatment, research, and prevention, but the sensitivity and specificity of tests for this purpose are uncertain.
  
  
  METHODS
  We used longitudinal specimens panels from 155 persons identified prior to HIV seroconversion to assess antibody-based methods for classifying persons as within 30, 60, or 90 days of seroconversion, including 2 incidence assays, a less-sensitive (LS) enzyme immunoassay (EIA), and the BED assay.
  
  
  RESULTS
  Sensitivity and specificity, respectively, for identifying persons within 30 days of seroconversion were: 34%-57% and 98%-100% for 2 standard EIAs (employing a signal-to-cutoff ≤4.0; ≥1.0 defines HIV positive), 84% and 73% for the LS-EIA (≤0.2 cutoff), 88% and 72% for the BED (≤0.2 cutoff), and 43%-58% and 98% (≤3 bands) for 2 Western blot (WB) assays. By area under the receiver operator curves, the best test for identifying persons within 30 days of seroconversion was the number of bands on the Bio-Rad WB (0.90); within 60 days, the LS-EIA and BED (both 0.85); and for persons within 90 days the BED (0.86).
  
  
  CONCLUSIONS
  Standard EIAs, Western blots, and HIV incidence assays provide useful information for identifying persons 30 to 90 days after seroconversion.
authors.affiliation: University of California, San Francisco, CA, USA. rhecht@php.ucsf.edu
authors.forename: Frederick M
authors.initials: FM
authors.lastname: Hecht
authors.forename: Robert
authors.initials: R
authors.lastname: Wellman
authors.forename: Michael P
authors.initials: MP
authors.lastname: Busch
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Pilcher
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Norris
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Margolick
authors.forename: Ann C
authors.initials: AC
authors.lastname: Collier
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Little
authors.forename: Martin
authors.initials: M
authors.lastname: Markowitz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Routy
authors.forename: Sarah
authors.initials: S
authors.lastname: Holte
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western / Q000379:methods*; D005260:Female; D015483:HIV Antibodies / Q000502:physiology*; D015658:HIV Infections / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D013997:Time Factors; D055815:Young Adult
chemical_list: D015483:HIV Antibodies