title: DNA capturing machinery through spore-displayed proteins.
abstract: OBJECTIVE
  The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin-displayed spores as a molecular machinery.
  
  
  RESULTS
  Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of Bacillus thuringiensis spores and as a diagnosis method for DNA detection. As a proof-of-concept, four pathogenic bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Klebsiella pneumonia were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson's disease were also used for the detection of single nucleotide polymorphism (SNP) in this system.
  
  
  CONCLUSIONS
  This strategy, utilizing streptavidin-displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson's disease.
  
  
  CONCLUSIONS
  This approach could be useful as a simple platform for developing sensitive spore-based biosensors for any desired DNA targets in diagnostic applications.
authors.affiliation: BioProcess Engineering Research Center, KAIST, Daejeon, Korea.
authors.forename: T J
authors.initials: TJ
authors.lastname: Park
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: J-G
authors.initials: JG
authors.lastname: Pan
authors.forename: H-C
authors.initials: HC
authors.lastname: Jung
authors.forename: J Y
authors.initials: JY
authors.lastname: Park
authors.forename: J P
authors.initials: JP
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
mesh_terms: D040981:Acinetobacter baumannii / Q000235:genetics; D001413:Bacillus thuringiensis / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D006527:Hepatolenticular Degeneration / Q000235:genetics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D020641:Polymorphism, Single Nucleotide; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013171:Spores, Bacterial / Q000235:genetics; D019809:Streptavidin / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D019809:Streptavidin