title: Bioactivity-guided fractionation for the butyrylcholinesterase inhibitory activity of furanocoumarins from Angelica archangelica L. roots and fruits.
abstract: Isolation and identification of the inhibitors of butyrylcholinesterase (BChE), obtained from the extracts of roots and fruits of Angelica archangelica L., are reported. Our results confirmed the weak inhibitory effect of Angelica roots on acetylcholinesterase activity. BChE inhibition was much more pronounced at a concentration of 100 μg/mL for hexane extracts and attained a higher rate than 50%. The TLC bioautography guided fractionation and spectroscopic analysis led to the isolation and identification of imperatorin from the fruit's hexane extract and of heraclenol-2'-O-angelate from the root's hexane extract. Both compounds showed significant BChE inhibition activity with IC(50) = 14.4 ± 3.2 μM and IC(50) = 7.5 ± 1.8 μM, respectively. Only C8-substituted and C5-unsubstituted furanocoumarins were active, which could supply information about the initial structures of specific BChE inhibitors.
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Wszelaki
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Paradowska
authors.forename: Marta K
authors.initials: MK
authors.lastname: Jamróz
authors.forename: Sebastian
authors.initials: S
authors.lastname: Granica
authors.forename: Anna K
authors.initials: AK
authors.lastname: Kiss
mesh_terms: D029970:Angelica archangelica; D002091:Butyrylcholinesterase / Q000187:drug effects*; D005591:Chemical Fractionation; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D005638:Fruit / Q000737:chemistry*; D011564:Furocoumarins / Q000302:isolation & purification* / Q000494:pharmacology; D006586:Hexanes; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*
chemical_list: D002800:Cholinesterase Inhibitors; D011564:Furocoumarins; D006586:Hexanes; D010936:Plant Extracts; D002091:Butyrylcholinesterase