title: Heat shock protein 60 from Klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone.
abstract: OBJECTIVE
  Bacterial heat shock proteins can have anti-apoptotic effects on human cells. We investigated whether enterobacterial HSP60 can protect peripheral blood mononuclear cells (PBMC) from DXM-induced apoptosis and if this effect requires cytoskeleton participation.
  
  
  METHODS
  Anti-apoptotic effect from enterobacterial HSP60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before DXM induction. Percentage of apoptotic cells was determined by SubG0 peak and TUNEL techniques in a flow cytometer.
  
  
  RESULTS
  Our results showed significant protective effect of HSP60 Klebsiella pneumoniae and E. coli, in the DXM-induced apoptosis in PBMC. Similar results were obtained with recombinant human HSP60. The same protective effect of proteins was observed in CD4+ and CD8 + T cell subpopulations. To analyze if enterobacterial HSP60 need internalization to have the anti-apoptotic effect, we used cytoskeleton inhibitors such as: nocodazole, cytochalasin D and amiloride, the three inhibitors significantly affected the protective role of HSP60 in apoptosis induced with DXM. Results suggest that the protective effect of HSP60 K. pneumoniae and E. coli requires the participation of contractile structures for the internalization of this protein by the cells, we suggest that the internalization of enterobacterial HSP60 could be carry out by macropinocytosis.
  
  
  CONCLUSIONS
  We report for the first time that K. pneumoniae and E. coli HSP60 have protective effect in the apoptosis induced with DXM in PBMC from healthy subjects and that this effect requires the internalization of the protein with active participation of the cytoskeleton.
authors.affiliation: Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico. yolaort06@yahoo.com.mx
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Ortega-Ortega
authors.forename: Ethel Awilda
authors.initials: EA
authors.lastname: García-Latorre
authors.forename: Ruth Angélica
authors.initials: RA
authors.lastname: Lezama
authors.forename: Julieta
authors.initials: J
authors.lastname: Luna-Herrera
authors.forename: Armando
authors.initials: A
authors.lastname: Vega-López
authors.forename: María Lilia
authors.initials: ML
authors.lastname: Domínguez-López
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D003907:Dexamethasone / Q000633:toxicity*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology / Q000503:physiopathology*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D003907:Dexamethasone