title: [Detoxication effect of water-soluble imprinted cross-linked chitosan on depleted uranium induced toxicity to renal cells].
abstract: To investigate whether a series of water-soluble cross-linked chitosan derivates synthesized in the guide of imprinting technology could be used as a uranium chelating agent to protect cells exposed to depleted uranium (DU), the imprinted chitosan derivates with high UO2(2+) chelating ability were screened, and cell model of human renal proximal tubule epithelium cells (HK-2) exposed to DU (500 micromol.L-1) was built, chitosan derivates (400 mg.L-1 ) was added to test group and diethylenetriaminepentaacetic acid (DTPA, 50 mg.L-1) was added to positive control group. The results showed that three Cu2+ imprinted chitosan derivates had higher uranium chelating ability (>49 microg.mg-1) than chitosan and non-imprinted chitosan derivates. Compared to the cells exposed to DU only, survival of cells in group added chitosan derivates rose up significantly (increased from 57.3% to 88.7%, and DTPA to 72.6%), and DU intracellular accumulation decreased, membrane damage and DNA damage also eased. Among the imprinted chitosan derivates, Cu2+ imprinted penta dialdehyde cross-linked carboxymethyl chitosan (Cu-P-CMC) was the best, and better than DTPA. From ultrastructure observation, the DU precipitates of test group added Cu-P-CMC were most grouped in a big hairy clusters in a string together outside cells. It is possible that the DU-chitosan derivates precipitates are too big to enter into cells, and from this way, the DU uptake by cells decreased so as to detoxication.
authors.affiliation: College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Chang-qi
authors.initials: CQ
authors.lastname: Zhao
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002614:Chelating Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D004249:DNA Damage / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000648:ultrastructure; D006801:Humans; D008658:Inactivation, Metabolic; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D014501:Uranium / Q000633:toxicity*; D014867:Water
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D003432:Cross-Linking Reagents; C514968:carboxymethyl-chitosan; D014867:Water; D014501:Uranium; D003300:Copper; D048271:Chitosan