title: Mandibular hematoma cells as a potential reservoir for osteoprogenitor cells in fractures.
abstract: OBJECTIVE
  We hypothesized that cells within the mandibular fracture hematoma played an important role in mandibular fracture healing. The objective of this study was to analyze cells in human mandibular fracture hematoma.
  
  
  METHODS
  We isolated and analyzed human mandibular fracture hematoma cells (MHCs) and investigated whether MHCs had multilineage mesenchymal differentiation capacity in vitro, similar to bone marrow stromal cells (BMSCs).
  
  
  RESULTS
  Cell-surface markers showed that the adherent MHCs expressed mesenchymal stem cell-related markers, namely CD29, CD44, CD105, and CD166, while lacking hematopoietic markers CD14, CD34, CD45, and CD133. The proliferative potential, osteogenic potential, and adipogenic potential of MHCs were comparable to those of BMSCs. In contrast, the chondrogenic potential of MHCs was inferior to that of BMSCs.
  
  
  CONCLUSIONS
  The role of the mandibular fracture hematoma could be as a presumptive local reservoir for osteogenic progenitors and thus contribute to intramembranous bone healing. Our findings may provide new insights into the mechanism of intramembranous bone healing in membranous bone fractures.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Miwa
authors.forename: Yoshitada
authors.initials: Y
authors.lastname: Sakai
authors.forename: Takahiro
authors.initials: T
authors.lastname: Niikura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nikura
authors.forename: Sang Yang
authors.initials: SY
authors.lastname: Lee
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oe
authors.forename: Takashi
authors.initials: T
authors.lastname: Iwakura
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kurosaka
authors.forename: Takahide
authors.initials: T
authors.lastname: Komori
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology; D000368:Aged; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002478:Cells, Cultured; D006406:Hematoma / Q000209:etiology* / Q000473:pathology; D006801:Humans; D008297:Male; D008337:Mandibular Fractures / Q000150:complications* / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000502:physiology; D055815:Young Adult
chemical_list: D012333:RNA, Messenger