title: Dose of 3-methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells.
abstract: Polycyclic aromatic hydrocarbon (PAH) compounds including 3-methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3-methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l-ascorbic acid daily and intraperitoneal injections of 10 mg 3-methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium-dependent vitamin C transporter (SVCTs) 1 and the l-ascorbic acid concentration in 3-methylcholanthrene-treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3-methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics-metabolizing, such as the induction of cytochrome P450 family.
authors.affiliation: Institute of Environmental Science for Human Life, Ochanomizu University, 2-1-1 Otsuka, Bunkyo, Tokyo 1128610, Japan. sone.yasuko@ocha.ac.jp
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sone
authors.forename: Etsuko
authors.initials: E
authors.lastname: Ueta
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Sannomaru
authors.forename: Noriko
authors.initials: N
authors.lastname: Miyake
authors.forename: Hirohito
authors.initials: H
authors.lastname: Sone
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Otsuka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kondo
authors.forename: Tadao
authors.initials: T
authors.lastname: Kurata
authors.forename: Emiko
authors.initials: E
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000378:metabolism* / Q000652:urine; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D051272:Glucose Transporter Type 1 / Q000235:genetics; D056945:Hep G2 Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008748:Methylcholanthrene / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D060888:Real-Time Polymerase Chain Reaction; D060347:Sodium-Coupled Vitamin C Transporters / Q000235:genetics*
chemical_list: D004785:Environmental Pollutants; D051272:Glucose Transporter Type 1; D012333:RNA, Messenger; D060347:Sodium-Coupled Vitamin C Transporters; D008748:Methylcholanthrene; D019363:Cytochrome P-450 CYP1A1; D001205:Ascorbic Acid