title: Third lineage of rodent eimerians: morphology, phylogeny and re-description of Eimeria myoxi (Apicomplexa: Eimeriidae) from Eliomys quercinus (Rodentia: Gliridae).
abstract: Coccidian oocysts from feces of 46 individuals of the garden dormouse, Eliomys quercinus (Rodentia: Gliridae), were morphologically and molecularly characterized. Both morphological and sequence data (18S rDNA and ORF 470) showed low variability, indicating that all samples represent a single species. By comparison with published morphological descriptions of coccidia from glirid rodents, we determined that the samples represent Eimeria myoxi. Molecular data suggest that this species does not fall within the 2 known rodent-specific groups but branches as a third independent lineage. However, its exact position in respect to other eimerian clusters could not be established due to the lack of phylogenetic information at this taxonomic level for the 18S rRNA and ORF 470 genes. Based on these results, we provide a re-description of Eimeria myoxi, which contains morphological and molecular characteristics sufficient for its further unequivocal identification.
authors.affiliation: Department of Parasitology, Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic. janaq@centrum.cz
authors.forename: Jana
authors.initials: J
authors.lastname: Kvičerová
authors.forename: Václav
authors.initials: V
authors.lastname: Mikeš
authors.forename: Václav
authors.initials: V
authors.lastname: Hypša
mesh_terms: D000818:Animals; D002452:Cell Count; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology*; D016054:DNA, Protozoan / Q000032:analysis; D004539:Eimeria / Q000166:cytology / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000032:analysis; D005243:Feces / Q000469:parasitology; D005260:Female; D008853:Microscopy; D058889:Molecular Typing; D046330:Myoxidae / Q000469:parasitology*; D033661:Oocysts / Q000166:cytology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D012376:Rodent Diseases / Q000175:diagnosis / Q000469:parasitology*; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV