title: [Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells].
abstract: This study is to find out the induction by sodium nitrite of epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells, SMMC-7721. After treatment of SMMC-7721 with 0.25 - 25 mmol.L-1 sodium nitrite for 48 h, the assays used include enzyme-linked immunosorbent assay (ELISA) for evaluation of TGF-beta1, IL-6 and IL-8 level in the conditioned medium, phase-contrast microscopy for morphology observation, and scratch wound healing as well as transwell migration assays for measurement of migration and metastatic potential. Additionally, the hallmarks of EMT, p-AKT and its downstream signaling molecules were examined by Western blotting. The results showed that TGF-beta1 secreted by SMMC-7721 elevated significantly in a dose-dependent fashion, whereas the increased IL-8 and IL-6 did not show dose-dependent response. The EMT was induced by exposure of SMMC-7721 with 0.25 mmol.L-1 of sodium nitrite, which was characterized by increased level of Vimentin, decreased E-cadherin and elevated activity of migration and metastatic potential. The results suggest that sodium nitrite could induce SMMC-7721 EMT by increased secretion of TGF-beta1 and IL-8.
authors.affiliation: The Institute of Environmental Medicine, Medical College, Henan University, Kaifeng 475004, China.
authors.forename: Yu-dong
authors.initials: YD
authors.lastname: Wang
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Fu
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Li
authors.forename: Chao-shen
authors.initials: CS
authors.lastname: Huang-Fu
mesh_terms: D015820:Cadherins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D004305:Dose-Response Relationship, Drug; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012977:Sodium Nitrite / Q000008:administration & dosage / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D051879:Twist-Related Protein 1 / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D015820:Cadherins; D015850:Interleukin-6; D016209:Interleukin-8; D016328:NF-kappa B; D053773:Transforming Growth Factor beta1; D051879:Twist-Related Protein 1; D014746:Vimentin; D051057:Proto-Oncogene Proteins c-akt; D012977:Sodium Nitrite