title: Survival and differentiation of neuroepithelial stem cells on chitosan bicomponent fibers.
abstract: Chitosan is a popular biomaterial used in tissue engineering. Fibers of chitosan could provide a favorable anatomical substrate for cell growth which provides a promising application for axonal regeneration during peripheral injury. Neuroepithelial stem cells (NEPs) are the most primitive neural stem cells with multipotential for neuronal and glia differentiation. To assess the biocompatibility between NEPs and chitosan fibers, and to explore whether the NEPs have the ability to differentiation on chitosan fibers, NEPs were harvested from the neural tube and seeded on chitosan fibers in in vitro culture. The biocompatibility of chitosan fibers was tested by MTT assays. The growth and survival were observed by light and scanning electronic microscope at different times in culture. And, the differentiation of NEPs was examined by immunocytochemical staining. The results indicated that NEPs could grow on the chitosan fibers and attach firmly to the surface of fibers. On chitosan fibers, NEPs could differentiate into neurons and glia. Our study demonstrated that chitasan fibers had a good biocompatibility with NEPs which affords a potential alternative for the repair of peripheral nerve injury.
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Anatomy, Shandong University School of Medicine, Jinan 250012, Shandong, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Fang
authors.forename: Qing
authors.initials: Q
authors.lastname: Gao
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Liu
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Qi
authors.forename: Gui-Bao
authors.initials: GB
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Li
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Sun
authors.forename: Jin-Hao
authors.initials: JH
authors.lastname: Sun
authors.forename: Ying-Mao
authors.initials: YM
authors.lastname: Gao
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002454:Cell Differentiation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D046569:Neuroepithelial Cells / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan; D010751:Phosphopyruvate Hydratase