title: Protective effect of aqueous garlic extract against lead-induced hepatic injury in rats.
abstract: Effect of aqueous extract of garlic on hepatic injury due to lead-induced oxidative stress in experimental rats has been investigated. Lead acetate (LA) at a dose of 15 mg/kg body wt was administered ip to rats for 7 consecutive days to induce hepatic injury. Freshly prepared aqueous garlic extract (AGE) at a dose of 50 mg/kg body wt was fed orally to rats 1 h before LA treatment for similar period. LA treatment caused hepatic injury as evident from increased activities of serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase (ALP), increased serum bilirubin level and damage in the tissue morphology. Lead-induced oxidative stress in liver was evident from increased levels of lipid peroxidation and reduced glutathione. The decreased activity of superoxide dismutase (SOD) and an increased activity of catalase as well as an increased activity of xanthine oxidase (XO) indicate generation and possible accumulation of reactive oxygen intermediates. Furthermore, altered activities of lactate dehydrogenase (LDH), isocitrate dehydrogenase (ICDH), alpha-keto glutarate dehydrogenase (alpha-KGDH) and succinate dehydrogenase (SDH) also indicate an impaired substrate utilization and generation of oxidative stress. All these changes were found to be mitigated when the rats were pre-treated with the AGE. Results indicate that AGE has the potential to ameliorate lead-induced hepatic injury due to oxidative stress in rats. The protective effects may be due to the antioxidant properties of AGE and may have future therapeutic relevance.
authors.affiliation: Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata 700 009, India.
authors.forename: Debamita
authors.initials: D
authors.lastname: Kilikdar
authors.forename: Debasri
authors.initials: D
authors.lastname: Mukherjee
authors.forename: Elina
authors.initials: E
authors.lastname: Mitra
authors.forename: Arnab K
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Anjali
authors.initials: A
authors.lastname: Basu
authors.forename: Ananga Mohan
authors.initials: AM
authors.lastname: Chandra
authors.forename: Debasish
authors.initials: D
authors.lastname: Bandyoapdhyay
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control*; D005737:Garlic / Q000737:chemistry*; D007854:Lead / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008111:Liver Function Tests; D008297:Male; D009942:Organometallic Compounds / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D053147:Plant Exudates / Q000008:administration & dosage / Q000627:therapeutic use*; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D014867:Water
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D009942:Organometallic Compounds; D053147:Plant Exudates; D020011:Protective Agents; D014867:Water; D007854:Lead; C008261:lead acetate