title: Association of MMP-2 and MMP-9 with clinical outcome of neurocysticercosis.
abstract: Matrix metalloproteinases (MMPs) are the major endopeptidases involved in proteolysis of blood brain barrier (BBB) during central nervous system (CNS) infections. The present study detected serum levels and activities of MMP-2 and MMP-9 in patients with neurocysticercosis (NCC) and their association with symptomatic disease. In total, 68 individuals with NCC (36 symptomatic patients with active seizures and 32 asymptomatic individuals) and 37 healthy controls were enrolled for the study. Serum MMP-2 and MMP-9 levels and their activities were measured by ELISA and gel zymography respectively. Mean serum MMP-2 levels (ng/ml) were higher both in asymptomatic and symptomatic NCC cases compared to healthy controls. However, significantly higher levels of serum MMP-9 (ng/ml) were detected only in symptomatic NCC patients compared to asymptomatic NCC cases and healthy controls. Levels of both MMPs positively correlated with symptomatic NCC. Serum MMP-2 activities were significantly higher in symptomatic and asymptomatic NCC compared to healthy controls whereas serum MMP-9 activity was significantly associated with symptomatic NCC compared to healthy controls and asymptomatic NCC. In conclusion, the elevated level of MMP-9 in serum appears to play an important role in the development of symptoms i.e. active seizures in patients with NCC. However, further studies are needed to elucidate its precise role in disease pathogenesis.
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow-226014, India.
authors.forename: Avantika
authors.initials: A
authors.lastname: Verma
authors.forename: Kashi N
authors.initials: KN
authors.lastname: Prasad
authors.forename: Kishan K
authors.initials: KK
authors.lastname: Nyati
authors.forename: Satyendra K
authors.initials: SK
authors.lastname: Singh
authors.forename: Aloukick K
authors.initials: AK
authors.lastname: Singh
authors.forename: Vimal K
authors.initials: VK
authors.lastname: Paliwal
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Gupta
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000097:blood; D001812:Blood-Brain Barrier / Q000469:parasitology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007194:India; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D020019:Neurocysticercosis / Q000097:blood* / Q000150:complications / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000503:physiopathology; D059748:Proteolysis; D012640:Seizures / Q000097:blood* / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology / Q000469:parasitology / Q000503:physiopathology; D013621:Taenia / Q000502:physiology*; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9