title: Evaluation of chemopreventive activity of glutamine by the comet and the micronucleus assay in mice's peripheral blood.
abstract: This research has evaluated the effects of enteral supplementation of glutamine in clastogens and genotoxic damages caused by the acute administration of cisplatin. For this, it was utilized Swiss mice distributed in eight experimental groups: control, cisplatin, glutamine, in three different doses and the combination of these with cisplatin. The results show that the glutamine was present in neither genotoxic nor mutagenic activity. When in association with glutamine and cisplatin, in simultaneous treatment, it was verified the frequency decreased of micronuclei and comets. The damage reduction percentages to the micronucleus ranged from 95.4 to 91.8% after 24h of administration of these compounds and 76.7 to 56.8% after 48h. In the same time the damage reduction percentages to the comet test ranged from 117.0 to 115.0%. The results suggest that glutamine is capable of preventing genotoxic and mutagenic damage according to the experimental design proposed.
authors.affiliation: Departamento de Nutrição, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; Departamento de Biomedicina, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; CENUGEN - Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia (UniFil), Londrina (PR), Brazil; Departamento de Biologia Geral, Universidade Estadual de Londrina (UEL), Londrina (PR), Brazil; Programa de Pós-graduação em Biologia Celular e Molecular, Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP), Rio Claro (SP), Brazil.
authors.forename: Rodrigo Juliano
authors.initials: RJ
authors.lastname: Oliveira
authors.forename: Evelyn
authors.initials: E
authors.lastname: Baise
authors.forename: Mariana de Oliveira
authors.initials: Mde O
authors.lastname: Mauro
authors.forename: João Renato
authors.initials: JR
authors.lastname: Pesarini
authors.forename: Renata
authors.initials: R
authors.lastname: Matuo
authors.forename: Ariane Fernanda da
authors.initials: AF
authors.lastname: Silva
authors.forename: Lúcia Regina
authors.initials: LR
authors.lastname: Ribeiro
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Mantovani