title: Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation.
abstract: We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels.
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne, Parkville 3010, Victoria, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Kramski
authors.forename: A J
authors.initials: AJ
authors.lastname: Gaeguta
authors.forename: G F
authors.initials: GF
authors.lastname: Lichtfuss
authors.forename: R
authors.initials: R
authors.lastname: Rajasuriar
authors.forename: S M
authors.initials: SM
authors.lastname: Crowe
authors.forename: M A
authors.initials: MA
authors.lastname: French
authors.forename: S R
authors.initials: SR
authors.lastname: Lewin
authors.forename: R J
authors.initials: RJ
authors.lastname: Center
authors.forename: D F J
authors.initials: DF
authors.lastname: Purcell
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D018988:Bacterial Translocation; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000097:blood; D020459:Genes, rRNA; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D008070:Lipopolysaccharides / Q000097:blood; D010949:Plasma / Q000737:chemistry / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013223:Statistics as Topic
chemical_list: D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D012336:RNA, Ribosomal, 16S