title: Antioxidant acitivity of low molecular weight hyaluronic acid.
abstract: Two polysaccharides, low molecular weight hyaluronic acid-1 (LMWHA-1) and LMWHA-2, with their molecular weight of 1.45×10(5) and 4.52×10(4)Da, respectively, were prepared from high molecular weight hyaluronic acid (HA,1.05×10(6)Da). LMWHA-1, LMWHA-2 and HA were studied for their antioxidant activities. In vitro antioxidant assay, LMWHA showed strong inhibition of lipid peroxidation and scavenging activities of hydroxyl radical, moderate 1,1-diphenyl-2-picryldydrazyl radical and superoxide anion scavenging activity. In addition, the LMWHA-1 exhibited much stronger antioxidant activity than LMWHA-2 and HA. For antioxidant testing in vivo, LMWHA-1, LMWHA-2 and HA were orally administrated over a period of 7days in a cyclophosphamide(CY) induced immunosuppressed mice model. As results, administration of LMWHA was able to overcome CY-induced immunosuppression and significantly raised the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) in immunosuppressed mice. The results showed that the LMWHA, possessing pronounced free radical scavenging and antioxidant activities.
authors.affiliation: Department of Biotechnology and Food Engineering, Bengbu College, Bengbu 233030, PR China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Ke
authors.forename: Lanping
authors.initials: L
authors.lastname: Sun
authors.forename: Deliang
authors.initials: D
authors.lastname: Qiao
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D002374:Catalase / Q000097:blood; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D006820:Hyaluronic Acid / Q000494:pharmacology*; D016867:Immunocompromised Host; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D008315:Malondialdehyde; D006820:Hyaluronic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase