title: Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography.
abstract: Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with co-registered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy.
authors.affiliation: The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, M018, 35 Stirling Highway, Crawley, Western Australia 6009, Australia. bklyen@ee.uwa.edu.au
authors.forename: Blake R
authors.initials: BR
authors.lastname: Klyen
authors.forename: Thea
authors.initials: T
authors.lastname: Shavlakadze
authors.forename: Hannah G
authors.initials: HG
authors.lastname: Radley-Crabb
authors.forename: Miranda D
authors.initials: MD
authors.lastname: Grounds
authors.forename: David D
authors.initials: DD
authors.lastname: Sampson
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004195:Disease Models, Animal; D005070:Evans Blue; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000473:pathology; D020388:Muscular Dystrophy, Duchenne / Q000473:pathology*; D009336:Necrosis; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
chemical_list: D004396:Coloring Agents; D005070:Evans Blue