title: [Study on transportation of Xiaochaihu Tang in Caco-2 cell model].
abstract: OBJECTIVE
  To study the transportation of Xiaochaihu Tang in Caco-2 cell model.
  
  
  METHODS
  The safety concentration of Xiaochaihu Tang in Caco-2 cells was determined by MTT assay. Then the Caco-2 cell model was used to investigate the bi-directional transportation of Xiaochaihu Tang. The multicomponents of Xiaochaihu Tang and the influence of time were measured by high performance liquid chromatography (HPLC).
  
  
  RESULTS
  The P(app) values of wogonoside and wogonin were (1.23 +/- 0.09) x 10(-6), (1.07 +/- 0.89) x 10(-5) cm x s(-1) from the AP side to BL side, and (2.12 +/- 0.19) x 10(-6) and (7.12 +/- 1.02) x 10(-6) cm x s(-1) from the BL side to AP side, respectively. The P(appAP --> BL)/P(app BL --> AP) ratio of wogonoside and wogonin were 0.58 and 1.49, respectively. Baicalin, baicalein and glycyrrhizic acid could not permeate the Caco-2 cell model.
  
  
  CONCLUSIONS
  The transportation of wogonoside and wogonin in Caco-2 cell model may be a passive transportation.
authors.affiliation: Institute of Pharmacology and Toxicology, Key Laboratory of Biological Evaluation of Traditional Chinese Medicine Quality of the Administration of Traditional Chinese Medicine, Sichuan Provincial Key Laboratory of the Quality and Innovation Research of Chinese Materia Medica, Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China. yanlc9722@163.com
authors.forename: Liangchun
authors.initials: L
authors.lastname: Yan
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Liu
authors.forename: Junning
authors.initials: J
authors.lastname: Zhao
authors.forename: Yueqi
authors.initials: Y
authors.lastname: Bi
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yi
mesh_terms: D001693:Biological Transport, Active; D018938:Caco-2 Cells / Q000378:metabolism*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D044950:Flavanones / Q000032:analysis / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism; D005960:Glucosides / Q000032:analysis / Q000378:metabolism*; D019695:Glycyrrhizic Acid / Q000032:analysis / Q000378:metabolism; D006801:Humans
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C461320:shosaiko-to; C038044:baicalin; C006680:baicalein; D019695:Glycyrrhizic Acid; C473995:wogonoside; C085514:wogonin