title: Salivary gland transcriptome analysis in response to sugar feeding in malaria vector Anopheles stephensi.
abstract: In this study, we analyzed a small scale transcriptome of salivary glands in sugar fed female mosquitoes. Thirty five percent of the transcripts could not be assigned a function. Some of them may code for salivary gland specific products involved in sugar feeding. We identified and characterized two new putative cDNAs encoding a sugar transporter and a cAMP generating DAPIT (Diabetes-Associated proteins in insulin sensitive tissues). Down regulation of these two cDNAs in response to blood feeding suggest that both AsST and AsDAPIT salivary genes may specifically be involved in the facilitation of sugar metabolism and energy production. The inability to absorb or digest sugar may cause organ failure, improper functioning of nervous system, behavioral disorder and death. Further functional characterization of theses putative transcripts is under investigation to examine their role in the mosquito salivary glands.
authors.affiliation: Host-Parasite Interaction Biology Group, National Institute of Malaria Research, Sector-8, Dwarka, Delhi, India.
authors.forename: Rajnikant
authors.initials: R
authors.lastname: Dixit
authors.forename: Manmeet
authors.initials: M
authors.lastname: Rawat
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Kumar
authors.forename: Kailash C
authors.initials: KC
authors.lastname: Pandey
authors.forename: T
authors.initials: T
authors.lastname: Adak
authors.forename: Arun
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000378:metabolism*; D002241:Carbohydrates; D005247:Feeding Behavior; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007303:Insect Vectors / Q000235:genetics / Q000378:metabolism*; D008288:Malaria / Q000635:transmission; D008969:Molecular Sequence Data; D012469:Salivary Glands / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D002241:Carbohydrates; D019476:Insect Proteins