title: Cellular renewal and improvement of local cell effector activity in peritoneal cavity in response to infectious stimuli.
abstract: The peritoneal cavity (PerC) is a singular compartment where many cell populations reside and interact. Despite the widely adopted experimental approach of intraperitoneal (i.p.) inoculation, little is known about the behavior of the different cell populations within the PerC. To evaluate the dynamics of peritoneal macrophage (MØ) subsets, namely small peritoneal MØ (SPM) and large peritoneal MØ (LPM), in response to infectious stimuli, C57BL/6 mice were injected i.p. with zymosan or Trypanosoma cruzi. These conditions resulted in the marked modification of the PerC myelo-monocytic compartment characterized by the disappearance of LPM and the accumulation of SPM and monocytes. In parallel, adherent cells isolated from stimulated PerC displayed reduced staining for β-galactosidase, a biomarker for senescence. Further, the adherent cells showed increased nitric oxide (NO) and higher frequency of IL-12-producing cells in response to subsequent LPS and IFN-γ stimulation. Among myelo-monocytic cells, SPM rather than LPM or monocytes, appear to be the central effectors of the activated PerC; they display higher phagocytic activity and are the main source of IL-12. Thus, our data provide a first demonstration of the consequences of the dynamics between peritoneal MØ subpopulations by showing that substitution of LPM by a robust SPM and monocytes in response to infectious stimuli greatly improves PerC effector activity.
authors.affiliation: Departamento de Ciências Biológicas, Campus Diadema e Centro de Terapia Celular e Molecular (CTC-Mol), Universidade Federal de São Paulo, Diadema, São Paulo, Brasil.
authors.forename: Alexandra dos Anjos
authors.initials: Ados A
authors.lastname: Cassado
authors.forename: José Antônio Tavares
authors.initials: JA
authors.lastname: de Albuquerque
authors.forename: Luiz Roberto
authors.initials: LR
authors.lastname: Sardinha
authors.forename: Carina de Lima
authors.initials: Cde L
authors.lastname: Buzzo
authors.forename: Lucas
authors.initials: L
authors.lastname: Faustino
authors.forename: Rogério
authors.initials: R
authors.lastname: Nascimento
authors.forename: Eliver Eid Bou
authors.initials: EE
authors.lastname: Ghosn
authors.forename: Maria Regina D'Império
authors.initials: MR
authors.lastname: Lima
authors.forename: Jose Maria Mosig
authors.initials: JM
authors.lastname: Alvarez
authors.forename: Karina Ramalho
authors.initials: KR
authors.lastname: Bortoluci
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002473:Cell Wall / Q000737:chemistry; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006681:HLA-D Antigens / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D009569:Nitric Oxide / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology* / Q000382:microbiology*; D012995:Solubility; D014349:Trypanosoma cruzi / Q000166:cytology / Q000502:physiology*; D015054:Zymosan / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000943:Antigens, Differentiation; D006681:HLA-D Antigens; D008070:Lipopolysaccharides; C036763:monocyte-macrophage differentiation antigen; D018664:Interleukin-12; D009569:Nitric Oxide; D015054:Zymosan