title: neo-Clerodane diterpenes from Ajuga ciliata Bunge and their neuroprotective activities.
abstract: Three new (1-3) and three known (4-6) neo-clerodane diterpenes have been isolated from the whole plants of Ajuga ciliata Bunge. The structures of the new compounds were elucidated as (12S)-1β,6α,19-triacetoxy-18-chloro-4α,12-dihydroxy-neo-clerod-13-en-15,16-olide (1), (12S,2'S)-12,19-diacetoxy-18-chloro-4α,6α-dihydroxy-1β-(2-methylbutanoyloxy)-neo-clerod-13-en-15,16-olide (2), and (12S)-6α,18,19-triacetoxy-4α,12-dihydroxy-1β-tigloyloxy-neo-clerod-13-en-15,16-olide (3), on the basis of spectroscopic data analysis. All the diterpenes were evaluated for the neuroprotective effects against MPP(+)-induced neuronal cell death in dopaminergic neuroblastoma SH-SY5Y cells and compounds 2-5 exhibited moderate neuroprotective effects.
authors.affiliation: College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300071, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guo
authors.forename: Yushan
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Yuanqiang
authors.initials: Y
authors.lastname: Guo
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Jin
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Wenbin
authors.initials: W
authors.lastname: Hou
authors.forename: Tiejun
authors.initials: T
authors.lastname: Zhang
mesh_terms: D015655:1-Methyl-4-phenylpyridinium; D031328:Ajuga / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects*; D002460:Cell Line; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015394:Molecular Structure; D009447:Neuroblastoma; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C570492:(12S)-1beta,6alpha,19-triacetoxy-18-chloro-4alpha,12-dihydroxy-neo-clerod-13-en-15,16-olide; C570495:(12S)-6alpha,18,19-triacetoxy-4alpha,12-dihydroxy-1beta-tigloyloxy-neo-clerod-13-en-15,16-olide; C570493:(12S,2'S)-12,19-diacetoxy-18-chloro-4alpha,6alpha-dihydroxy-1beta-(2-methylbutanoyloxy)-neo-clerod-13-en-15,16-olide; D045785:Diterpenes, Clerodane; D018696:Neuroprotective Agents; D010936:Plant Extracts; D015655:1-Methyl-4-phenylpyridinium