title: Ex vivo comparison of three hand sewn end-to-end anastomoses in normal equine jejunum.
abstract: BACKGROUND
  Although single layer techniques are preferred in man and small animals for small intestinal end-to-end anastomoses, double layer techniques are more popular in equine surgery. This study was undertaken to evaluate the ex vivo characteristics of 2 single layer anastomoses in comparison to the traditionally performed double layer anastomosis in equine jejunum.
  
  
  OBJECTIVE
  To compare ex vivo: 1) construction time; 2) bursting pressure; and 3) lumen size reduction of 3 suture patterns (double layer simple continuous/Cushing pattern [2C]; single layer Lembert pattern [1L]; and single layer Cushing pattern [1C]) for end-to-end equine jejunojejunostomies.
  
  
  METHODS
  End-to-end jejunojejunostomies were constructed using 2C (n = 7), 1L (n = 7) and 1C (n = 7) in harvested equine jejunum and construction times were recorded. Anastomosed and control segments were distended with gas until failure. Intraluminal pressure at failure and mode of failure were recorded. Lumen size reduction was calculated as a percentage decrease from control jejunum. Results were compared using an ANOVA and P<0.05 was considered significant.
  
  
  RESULTS
  The 1C anastomoses were faster to construct than the 1L anastomoses, which were faster to construct than the 2C anastomoses. There were no differences in bursting pressures between the different anastomoses and control jejunum. All anastomoses decreased lumen size from control values but there were no differences in lumen reduction between types of anastomoses.
  
  
  CONCLUSIONS
  Single layer anastomoses are faster to construct than double layer anastomoses, with the 1C being fastest. Single layer anastomoses are as strong and result in comparable lumen size reduction as traditional 2C anastomoses.
  
  
  CONCLUSIONS
  As the 1C anastomosis results in less exposed potentially adhesiogenic suture material than the 1L while providing adequate strength and similar luminal size reduction, the 1C may be better for equine small intestine anastomosis and further in vivo studies are warranted.
authors.affiliation: College of Veterinary Medicine, Department of Large Animal Medicine, The University of Georgia, GA, USA. cerisherlock@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Sherlock
authors.forename: W
authors.initials: W
authors.lastname: Lee
authors.forename: P O E
authors.initials: PO
authors.lastname: Mueller
authors.forename: R
authors.initials: R
authors.lastname: Eggleston
authors.forename: K
authors.initials: K
authors.lastname: Epstein
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods / Q000662:veterinary*; D000818:Animals; D005260:Female; D006736:Horses; D007583:Jejunum / Q000601:surgery*; D008297:Male; D013536:Suture Techniques / Q000662:veterinary*; D013997:Time Factors