title: Glyphosate-induced antioxidant imbalance in HaCaT: The protective effect of Vitamins C and E.
abstract: Roundup 3 plus(®), a glyphosate-based herbicide, is widely used in the ground, but its extensive use has posed a health risk in man. The aim of this study was firstly to investigate how glyphosate alone or included in Roundup 3 plus(®) affected the antioxidant defense system and lipid peroxidation of human cutaneous cells, and secondly, to evaluate the ameliorating effects of antioxidants, as Vitamin C (VitC) and Vitamin E (VitE), against Roundup 3 plus(®)-induced epidermal antioxidant impairment. Our results showed that glyphosate alone or included in Roundup 3 plus(®), induced significant changes in cellular antioxidant status as a glutathione depletion, enzymatic (catalase, glutathione-peroxidase and superoxide dismutase) disorders, and increased lipid peroxidation. VitC or VitE supplementation increased superoxide dismutase, glutathione-reductase and -peroxidase activities and reduced lipid peroxidation in Roundup 3 plus(®)-treated keratinocytes. These in vitro data indicated that VitC and VitE might have preventive effects against deleterious cutaneous cell damage caused by Roundup 3 plus(®).
authors.affiliation: Equipe des Sciences Séparatives et Biopharmaceutiques (EA 3924), Université de Franche-Comté UFR des Sciences Médicales et Pharmaceutiques, Laboratoire de Biologie Cellulaire, Place Saint Jacques, F-25030 Besançon Cedex, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Gehin
authors.forename: Catherine
authors.initials: C
authors.lastname: Guyon
authors.forename: Laurence
authors.initials: L
authors.lastname: Nicod