title: Effects of oleanolic acid on pulmonary morphofunctional and biochemical variables in experimental acute lung injury.
abstract: We analysed the effects of oleanolic acid (OA) on lung mechanics and histology and its possible mechanisms of action in experimental acute lung injury (ALI). BALB/c mice were randomly divided into Control (saline, ip) and ALI (paraquat, 25 mg/kg, ip) groups. At 1 h, both groups were treated with saline (SAL, 50 μl ip), OA (10 mg/kg ip), or dexamethasone (DEXA, 1 mg/kg ip). At 24 h, lung static elastance, viscoelastic pressure, and alveolar collapse reduced more after OA compared to DEXA administration. Tumour necrosis factor-α, macrophage migration inhibitory factor, interleukin-6, interferon-γ, and transforming growth factor-β mRNA expressions in lung tissue diminished similarly after OA or DEXA. Conversely, only OA avoided reactive oxygen species generation and yielded a significant decrease in nitrite concentration. OA and DEXA restored the reduced glutathione/oxidized glutathione ratio and catalase activity while increasing glutathione peroxidase induced by paraquat. In conclusion, OA improved lung morphofunction by modulating the release of inflammatory mediators and oxidative stress.
authors.affiliation: Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Raquel S
authors.initials: RS
authors.lastname: Santos
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Silva
authors.forename: Gisele P
authors.initials: GP
authors.lastname: Oliveira
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Cruz
authors.forename: Débora S
authors.initials: DS
authors.lastname: Ornellas
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Morales
authors.forename: Janaina
authors.initials: J
authors.lastname: Fernandes
authors.forename: Manuella
authors.initials: M
authors.lastname: Lanzetti
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Valença
authors.forename: Paolo
authors.initials: P
authors.lastname: Pelosi
authors.forename: Cerli R
authors.initials: CR
authors.lastname: Gattass
authors.forename: Patricia R M
authors.initials: PR
authors.lastname: Rocco
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D018925:Chemokines / Q000032:analysis / Q000096:biosynthesis; D004195:Disease Models, Animal; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009828:Oleanolic Acid / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D017382:Reactive Oxygen Species / Q000032:analysis / Q000276:immunology; D015656:Respiratory Mechanics / Q000187:drug effects* / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D018925:Chemokines; D017382:Reactive Oxygen Species; D009828:Oleanolic Acid