title: Production of a novel 9,12-dihydroxy-10(E)-eicosenoic acid from eicosenoic acid by Pseudomonas aeruginosa PR3.
abstract: Microbial conversions of unsaturated fatty acids often generate polyhydroxy fatty acids, giving them new properties such as higher viscosity and reactivity. A bacterial strain Pseudomonas aeruginosa (PR3) has been intensively studied to produce mono-, di-, and trihydroxy fatty acids from different 9-cis-monoenoic fatty acids such as oleic acid, ricinoleic acid, and palmitoleic acid. However, from the results and the postulated similar metabolic pathways involved in these transformations, it was assumed that the enzyme system involved in transformation of the monoenoic fatty acid by strain PR3 could utilize fatty acids with different chain lengths and locations of the double bond. In this study was used as a substrate for bioconversion by strain PR3 eicosenoic acid (C20:1, ω-9) containing a singular cis double bond at different positions from the carboxyl end as oleic acid, and it was confirmed that PR3 could produce a novel 9,12-dihydroxy-10(E)-eicosenoic acid (DED) with 6.2% yield from eicosenoic acid. The structure of DED was confirmed using GC-MS, FTIR, and NMR analyses. DED production was maximized at 72 h after the substrate was added to the 24 h culture. Some other nutritional factors were also studied for optimal production of DED.
authors.affiliation: School of Food Science and Biotechnology, Kyungpook National University, Daegu, South Korea 702-701.
authors.forename: Ka-Yeon
authors.initials: KY
authors.lastname: Back
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Sohn
authors.forename: Ching T
authors.initials: CT
authors.lastname: Hou
authors.forename: Hak-Ryul
authors.initials: HR
authors.lastname: Kim
mesh_terms: D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: C572290:9,12-dihydroxy-10(E)-eicosenoic acid; D005229:Fatty Acids, Monounsaturated; C572289:eicosenoic acid