title: [Simultaneous determination of acteoside, oleanolic acid and ursolic acid in flower of Campsis grandiflora by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for the determination of acteoside, oleanolic acid and ursolic acid in flowers of Campsis grandiflora.
  
  
  METHODS
  The analysis was carried out on an Agilent ZORBAX Eclipse XDB-18 column eluted with methanol and 0.1% phosphoric acid in gradient mode. The detection wavelength was set at 334 mn at 0-30 min and 210 nm at 30-60 min.
  
  
  RESULTS
  The peak areas and concentrations have a good linear relationship at 0.025 9-0.258 g x L(-1) for acteoside, 0.100-1.00 g x L(-1) for oleanolic acid and 0.104-1.04 g x L(-1) for ursolic acid, respectively. The average recoveries were 98.9%, 99.3% and 99.40%, respectively.
  
  
  CONCLUSIONS
  The method can determinate the concentration of acteoside, oleanolic acid and ursolic acid simultaneously. It can be used for the quality control of flower of C. grandiflora.
authors.affiliation: Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China. dafuyuan_hubei@163.com
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.forename: Yujie
authors.initials: Y
authors.lastname: Liu
authors.forename: Yu'an
authors.initials: Y
authors.lastname: Bi
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D029402:Bignoniaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D035264:Flowers / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D009828:Oleanolic Acid / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid; D059808:Polyphenols
chemical_list: D005960:Glucosides; D009828:Oleanolic Acid; D010636:Phenols; D014315:Triterpenes; D000097245:Ursolic Acid; C058956:acteoside; D059808:Polyphenols