title: Analgesic activity of the aqueous fraction from the ethanolic extract of Chrysanthemum indicum in mice.
abstract: The aqueous fraction (AF) of an ethanolic extract from Chrysanthemum indicum was evaluated for analgesic activity in mice using chemical and thermal models of nociception. Given orally, AF at doses of 300 and 600 mg/kg produced significant inhibitions on chemical nociception induced by intraperitoneal acetic acid, subplantar formalin/capsaicin injections and on thermal nociception in the tail-flick test and in the hot plate test. In the pentobarbital sodium-induced sleeping time test and the open-field test, AF neither significantly enhanced the pentobarbital sodium-induced sleeping time nor impaired the motor performance, indicating that the observed analgesic activity was unlikely due to sedation or motor abnormality. In a measurement of core body temperature, AF did not affect temperature within 80 min. Moreover, the effective dose (600 mg/kg) also showed no toxicity within 7 days. These results suggested further that AF produced analgesic activity possibly related to the flavonoid glycosides and phenolic glycosides in this fraction.
authors.affiliation: Department of Pharmacy, Shen-yang North Hospital, Shen-yang PR China. yufengchen1978@163.com
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Chen
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
authors.forename: Guo-Bing
authors.initials: GB
authors.lastname: Shi
authors.forename: Guan-Xin
authors.initials: GX
authors.lastname: Hou
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Qin-Chun
authors.initials: QC
authors.lastname: Zhao
mesh_terms: D019342:Acetic Acid; D000700:Analgesics; D000701:Analgesics, Opioid / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001831:Body Temperature; D002211:Capsaicin; D031202:Chrysanthemum / Q000737:chemistry*; D000431:Ethanol; D004987:Ethers; D006358:Hot Temperature; D006993:Hypnotics and Sedatives; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D010147:Pain Measurement; D010424:Pentobarbital; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D011930:Reaction Time / Q000187:drug effects; D012890:Sleep / Q000187:drug effects; D012997:Solvents; D014867:Water
chemical_list: D000700:Analgesics; D000701:Analgesics, Opioid; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004987:Ethers; D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; D009020:Morphine; D010424:Pentobarbital; D019342:Acetic Acid; D001241:Aspirin; D002211:Capsaicin