title: Evidence for age-related and individual-specific changes in DNA methylation profile of mononuclear cells during early immune development in humans.
abstract: Environment induced epigenetic effects on gene expression in early life are likely to play important roles in mediating the risk of several immune-related diseases. In order to investigate this fully, it is essential to first document temporal changes in epigenetic profile in disease-free individuals as a prelude to defining environmentally mediated changes. Mononuclear cells (MC) were collected longitudinally from a small number of females at birth, 1 year, 2.5 years and 5 years of age and examined for changes in genome-scale DNA methylation profiles using the Illumina Infinium HumanMethylation27 BeadChip array platform. MC from two males were included for comparative purposes. Flow cytometry was used to define MC cell populations in each sample in order to exclude this as the major driver of epigenetic change. The data underwent quality control and normalization within the R programming environment. Unsupervised hierarchical clustering of samples clearly delineated neonatal MC from all other ages. A further clear distinction was observed between 1 year and 5 year samples, with 2.5 year samples showing a mixed distribution between the 1 and 5 year groups. Gene ontology of probes significantly variable over the neonatal period revealed methylation changes in genes associated with cell surface receptor and signal transduction events. In the postnatal period, methylation changes were mostly associated with the development of effector immune responses and homeostasis. Unlike all other chromosomes tested, a predominantly genetic effect was identified as controlling maintenance of X-chromosome methylation profile in females, largely refractory to change over time. This data suggests that the primary driver of neonatal epigenome is determined in utero, whilst postnatally, multiple genetic and environmental factors are implicated in the development of MC epigenetic profile, particularly between the ages of 1-5 years, when the highest level of inter individual variation is apparent. This supports a model for differential sensitivity of specific individuals to disruption in the developing epigenome during the first years of life. Further studies are now needed to examine evolving epigenetic variations in specific cell populations in relation to environmental exposures, immune phenotype and subsequent disease susceptibility.
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia.
authors.forename: David J
authors.initials: DJ
authors.lastname: Martino
authors.forename: Meri K
authors.initials: MK
authors.lastname: Tulic
authors.forename: Lavinia
authors.initials: L
authors.lastname: Gordon
authors.forename: Megan
authors.initials: M
authors.lastname: Hodder
authors.forename: Tara R
authors.initials: TR
authors.lastname: Richman
authors.forename: Jessica
authors.initials: J
authors.lastname: Metcalfe
authors.forename: Susan L
authors.initials: SL
authors.lastname: Prescott
authors.forename: Richard
authors.initials: R
authors.lastname: Saffery
mesh_terms: D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D002675:Child, Preschool; D041321:Chromosomes, Human, X / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016000:Cluster Analysis; D019175:DNA Methylation; D004777:Environment; D044127:Epigenesis, Genetic; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015894:Genome, Human; D006706:Homeostasis; D006801:Humans; D007109:Immunity; D016130:Immunophenotyping; D007223:Infant; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008137:Longitudinal Studies; D020935:MAP Kinase Signaling System; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011786:Quality Control; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D013997:Time Factors
chemical_list: D011956:Receptors, Cell Surface