title: Inhibitory effects of interferon-gamma plasmid DNA on DMBA-TPA induced mouse skin carcinogenesis.
abstract: Interferon-gamma (IFN-γ) exhibits biological activities that are considered to have important roles in tumor suppression. Therefore, the IFN-γ gene is a potential candidate for in vivo cytokine gene therapy against skin cancer. The present study evaluated the efficacy of a hydrodynamics-based IFN-γ gene transfection for skin cancer treatment, in which the plasmid DNA encoding IFN-γ was administered into the tail vein of mice following 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate-induced skin carcinogenesis. Serum levels of IFN-γ were substantially elevated without liver toxicity. The mice injected with IFN-γ plasmid DNA displayed a marked reduction in papilloma numbers, suppressed proliferation of epidermal cells and induction of caspase-3-mediated apoptosis. These results suggest that the hydrodynamics-based transfection of IFN-γ plasmid DNA is a convenient and efficient means of skin cancer gene therapy.
authors.affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: J-H
authors.initials: JH
authors.lastname: Ko
authors.forename: B-G
authors.initials: BG
authors.lastname: Jung
authors.forename: Y-S
authors.initials: YS
authors.lastname: Park
authors.forename: B-J
authors.initials: BJ
authors.lastname: Lee
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D015316:Genetic Therapy; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000628:therapy*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
chemical_list: D015127:9,10-Dimethyl-1,2-benzanthracene; D007371:Interferon-gamma; D053148:Caspase 3; D013755:Tetradecanoylphorbol Acetate