title: Efficient in vitro myogenic reprogramming of human primary mesenchymal stem cells and endothelial cells by Myf5.
abstract: BACKGROUND
  The identification of a source of stem cells able to regenerate skeletal muscle was the goal of numerous studies with the aim to develop new therapeutic approaches for genetic muscle diseases or muscle injuries. A series of studies have demonstrated that stem cells derived from various tissues may have a role in the regeneration of damaged muscles, but this contribution is always very weak. Thus we established a project aiming to reprogramme non-muscle cells into the skeletal striated differentiation pathway.
  
  
  RESULTS
  We transduced several human primary adult stem or progenitor cells using a recombinant lentivirus containing the coding sequence of the Myf5 gene considered as a master gene for the determination of skeletal striated muscle. These original results are the first demonstration of a myogenic conversion of human mesenchymal and endothelial cells by Myf5.
  
  
  CONCLUSIONS
  The procedure described in the present paper could be used to develop new research protocols with the prospect of using these cells as therapeutic agents.
authors.affiliation: Inserm U1016, 75014 Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Dimicoli-Salazar
authors.forename: Frederique
authors.initials: F
authors.lastname: Bulle
authors.forename: Azzedine
authors.initials: A
authors.lastname: Yacia
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Massé
authors.forename: Serge
authors.initials: S
authors.lastname: Fichelson
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vigon
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics / Q000378:metabolism*
chemical_list: C497417:MYF5 protein, human; D051787:Myogenic Regulatory Factor 5