title: Proteomic investigation of 1,6-dimethoxyhexane testicular toxicity.
abstract: The aliphatic ether 1,6-dimethoxyhexane (DMH) was previously identified as a testicular toxicant. Testis protein extracts from control and DMH-treated rats were subjected to two-dimensional gel electrophoresis for comparison of protein expression profiles. MALDI-ToF peptide mass fingerprinting of differentially expressed proteins resulted in the conclusive identification of heat shock-related 70kDa protein 2 (HSP70.2), 60kDa heat shock protein, mitochondrial precursor (HSP60) and protein disulfide isomerase A3 precursor (ERp60). The potential involvement of these proteins in chemically induced perturbation of spermatogenesis and their utility as biomarkers of testicular toxicity are discussed in light of the knowledge currently available from the literature.
authors.affiliation: Systemic Toxicology and Pharmacokinetics Section, Environmental and Occupational Toxicology Division, Environmental Health Sciences Bureau, Health Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Pelletier
authors.forename: Sheila
authors.initials: S
authors.lastname: Masson
authors.forename: Yuan-Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Michael G
authors.initials: MG
authors.lastname: Wade
authors.forename: Susantha
authors.initials: S
authors.lastname: Mohottalage
authors.forename: Premkumari
authors.initials: P
authors.lastname: Kumarathasan
authors.forename: Renaud
authors.initials: R
authors.lastname: Vincent
authors.forename: Raymond
authors.initials: R
authors.lastname: Poon
authors.forename: Ih
authors.initials: I
authors.lastname: Chu