title: Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp.
abstract: Bacterial strains capable of utilizing methylphosphonic acid (MP) or glyphosate (GP) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. The strains isolated from MP-contaminated soils grew on MP and failed to grow on GP. One group of the isolates from GP-contaminated soils grew only on MP, while the other one grew on MP and GP. Strains Achromobacter sp. MPS 12 (VKM B-2694), MP degraders group, and Ochrobactrum anthropi GPK 3 (VKM B-2554D), GP degraders group, demonstrated the best degradative capabilities towards MP and GP, respectively, and were studied for the distribution of their organophosphonate catabolism systems. In Achromobacter sp. MPS 12, degradation of MP was catalyzed by C-P lyase incapable of degrading GP (C-P lyase I). Adaptation to growth on GP yielded the strain Achromobacter sp. MPS 12A, which retained its ability to degrade MP via C-P lyase I and was capable of degrading GP with formation of sarcosine, thus suggesting the involvement of a GP-specific C-P lyase II. O. anthropi GPK 3 also degraded MP via C-P lyase I, but degradation of GP in it was initiated by glyphosate oxidoreductase, which was followed by product transformation via the phosphonatase pathway.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Sviridov
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Shushkova
authors.forename: Nina F
authors.initials: NF
authors.lastname: Zelenkova
authors.forename: Natalya G
authors.initials: NG
authors.lastname: Vinokurova
authors.forename: Igor G
authors.initials: IG
authors.lastname: Morgunov
authors.forename: Inna T
authors.initials: IT
authors.lastname: Ermakova
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Leontievsky
mesh_terms: D042402:Achromobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005998:Glycine / Q000031:analogs & derivatives* / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D020621:Ochrobactrum anthropi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D000097797:Glyphosate
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005998:Glycine; D009943:Organophosphorus Compounds; D012336:RNA, Ribosomal, 16S; D000097797:Glyphosate; C032627:methylphosphonic acid