title: A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos.
abstract: Using the surf clam embryo, we investigated the effects of the combination of bromoform, chloroform, and tetrachloroethylene, three pollutants found in high concentrations in the municipal water supply in Brick, New Jersey. Exposure produced an increase in an isoform of the regulatory subunit (RII) of cAMP-dependent protein kinase, demonstrated by confocal microscopy and western blotting. Embryos showed an increase in RII where the primordial gill and ciliated velar epithelium are innervated. This increase correlated with increased ciliary activity, indicating a corresponding rise in the catalytic subunit. Treatment resulted in decreased threonine phosphorylation of actin. There was no effect on neurotransmitters or receptors of the serotonergic-dopaminergic nervous system. These effects occurred only with the ternary mixture. No significant effect was seen with individual or paired components. This is the first report showing that bromoform, chloroform, and tetrachloroethylene act synergistically to alter a key regulator of neuronal development.
authors.affiliation: Marine Biological Laboratory, Woods Hole, MA 02543, USA; Department of Obstetrics and Gynecology, Brown University School of Medicine, Women and Infants Hospital, Box G-B187, 171 Meeting St., Providence, RI 02912, USA.
authors.forename: Jill A
authors.initials: JA
authors.lastname: Kreiling
authors.forename: Raymond E
authors.initials: RE
authors.lastname: Stephens
authors.forename: Carol L
authors.initials: CL
authors.lastname: Reinisch