title: Cellular biocompatibility and biomechanical properties of N-carboxyethylchitosan/nanohydroxyapatite composites for tissue-engineered trachea.
abstract: To prepare an NCECS/nHA composite for tissue-engineered trachea and investigate its biomechanical and biocompatibile properties. Biomechanical tests were performed on dry and wet NCECS/nHA composite specimens prepared in vitro. The cell adhesion rate on each composite surface after 2, 6, and 12 hours of culture was calculated, and cell proliferation activity was measured using an MTT assay. NCECS/nHA composites exhibited satisfactory tensile strength and Young's modulus values. The adhesion rate of rabbit tracheal chondrocytes on NCECS/nHA surfaces reached 88.4% after 12 hours of culture. NCECS/nHA composites are promising scaffold materials for tissue-engineered trachea owing to satisfactory biocompatible and biomechanical properties.
authors.affiliation: Department of Cardiothoracic Surgery, College of Clinical Medicine, Yangzhou University, Yangzhou, Jiangsu Province, China. shihongcan@hotmail.com
authors.forename: Hongcan
authors.initials: H
authors.lastname: Shi
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Lu
authors.forename: Haijia
authors.initials: H
authors.lastname: Li
authors.forename: Linsong
authors.initials: L
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002448:Cell Adhesion / Q000187:drug effects; D048271:Chitosan / Q000009:adverse effects* / Q000737:chemistry; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D017886:Durapatite / Q000009:adverse effects* / Q000737:chemistry; D008422:Materials Testing; D055595:Mechanical Phenomena; D053761:Nanocomposites / Q000009:adverse effects* / Q000737:chemistry; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000009:adverse effects / Q000737:chemistry; D014132:Trachea / Q000166:cytology* / Q000187:drug effects
chemical_list: C068302:N-(carboxymethyl)chitosan; D048271:Chitosan; D017886:Durapatite