title: D1/D2 domain of large-subunit ribosomal DNA for differentiation of Orpinomyces spp.
abstract: This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.
authors.affiliation: Dairy Microbiology Division, National Dairy Research Institute, Karnal, India.
authors.forename: Sumit S
authors.initials: SS
authors.lastname: Dagar
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Priti
authors.initials: P
authors.lastname: Mudgil
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Singh
authors.forename: Anil K
authors.initials: AK
authors.lastname: Puniya
mesh_terms: D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D020085:Neocallimastigales / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S