title: Schistosoma mansoni population structure and persistence after praziquantel treatment in two villages of Bahia, Brazil.
abstract: Praziquantel has been used to treat schistosome infections since 1979 and currently is the only chemotherapeutic agent in production for this purpose, raising concerns about the potential for the emergence of drug resistance. In practice, 10-20% of infected patients will continue to excrete eggs after treatment. It is not understood to what degree this represents selection of a resistant population or incomplete elimination due to the presence of immature worms at the time of treatment. We used a population genetics approach to test whether or not persistent Schistosomamansoni parasites were drawn from the same population as susceptible parasites. In this study, stool samples were collected from 96% of individuals in two small Brazilian communities (populations 482 and 367) and examined for S.mansoni eggs. The combined prevalence of S.mansoni infections in the villages was 41%. Total egg DNA was extracted from each sample and was genotyped at 15 microsatellite markers. Day-to-day variation of the infrapopulation from an individual human host was low (median differentiation using Jost's D=0.010), so that a single stool was representative of the genotypes present in stool eggs, at least in the short term. Average pairwise analysis of D among all pre-treatment infrapopulations suggested moderate differentiation (mean D=0.082 and 0.122 for the two villages), whereas the pre-treatment component population differentiation between the two communities was 0.047. The differentiation of the component population remaining after treatment from the fully susceptible component population was low (mean D=0.007 and 0.020 for the two villages), suggesting that the persistent parasites were not selected by praziquantel treatment. We will continue to follow these communities for evidence of selection or changes in population structure.
authors.affiliation: Case Western Reserve University, Centre for Global Health and Diseases, Wolstein Research Building, 2301 Cornell Road, Cleveland, OH 44106, USA. reb6@case.edu
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Blanton
authors.forename: Walter A
authors.initials: WA
authors.lastname: Blank
authors.forename: Jackson M
authors.initials: JM
authors.lastname: Costa
authors.forename: Theomira M
authors.initials: TM
authors.lastname: Carmo
authors.forename: Eliana A
authors.initials: EA
authors.lastname: Reis
authors.forename: Luciano K
authors.initials: LK
authors.lastname: Silva
authors.forename: Lúcio M
authors.initials: LM
authors.lastname: Barbosa
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Test
authors.forename: Mitermayer G
authors.initials: MG
authors.lastname: Reis
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D011223:Praziquantel / Q000008:administration & dosage*; D012550:Schistosoma mansoni / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012555:Schistosomiasis mansoni / Q000188:drug therapy*; D012641:Selection, Genetic; D055815:Young Adult
chemical_list: D000871:Anthelmintics; D011223:Praziquantel