title: Endothelial nitric oxide synthase gene polymorphisms in preeclampsia with or without eclampsia in a Turkish population.
abstract: OBJECTIVE
  To evaluate endothelial nitric oxide synthase (eNOS) gene polymorphisms in preeclampsia with or without eclampsia in a Turkish population.
  
  
  METHODS
  Fifty-seven preeclamptic and 60 normotensive women were enrolled in the present study. The study focused on two functional variants: a variant in exon 7-G to T conversion at nucleotide position 894 resulting in the replacement of glutamic acid with aspartic acid at codon 298 (Glu298Asp) and a variant variable number of 27 bp tandem repeats in intron 4 (VNTR intron 4). Two polymorphisms in the maternal eNOS gene were characterized by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method. Preeclamptic pregnant women were evaluated into two subgroups according to the presence of eclampsia.
  
  
  RESULTS
  We demonstrated a significant difference in patients with preeclampsia in terms of Glu298Asp/eNOS genotype frequency. G/G homozygotes of Glu298Asp among healthy pregnancies were significantly frequent when T/T homozygotes were significantly frequent among preeclamptic pregnancies. We showed that G/T heterozygotes of Glu298Asp/eNOS gene were significantly frequent among preeclamptic pregnant women who did not develop eclampsia than in preeclamptic pregnant women with eclampsia.
  
  
  CONCLUSIONS
  Glu298Asp polymorphism in the eNOS gene could be an individual's risk factor and may modulate progression to an eclampsia complication of preeclampsia in the Turkish population.
authors.affiliation: Departments of Obstetrics and Gynecology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey. ebruozturkarslan@yahoo.com
authors.forename: Ebru
authors.initials: E
authors.lastname: Ozturk
authors.forename: Ozcan
authors.initials: O
authors.lastname: Balat
authors.forename: Sacide
authors.initials: S
authors.lastname: Pehlivan
authors.forename: Mete Gurol
authors.initials: MG
authors.lastname: Ugur
authors.forename: Cağlayan
authors.initials: C
authors.lastname: Ozcan
authors.forename: Tugce
authors.initials: T
authors.lastname: Sever
authors.forename: Seval
authors.initials: S
authors.lastname: Kul
mesh_terms: D000328:Adult; D004461:Eclampsia / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011446:Prospective Studies; D014421:Turkey
chemical_list: D052250:Nitric Oxide Synthase Type III