title: Coatings of one monomer molecularly imprinted polymers for open tubular capillary electrochromatography.
abstract: One monomer molecularly imprinted polymer coatings were first synthesized in fused silica capillary columns with 2-methacrylamidopropyl methacrylate (MAM) as single functional monomer in addition to a cross-linking monomer. Since MAM may generate no or little EOF, a strategy of precursor of polymerization, which does not interfere with the formation of defined imprints, was used to introduce an ionizable functional monomer to generate a stable electroosmotic flow for electrochromatography (CEC) by post-polymerization hydrolization. The resulting MAM-based open-tubular imprinted capillary was able to separate enantiomers by means of CEC. The resolution of enantiomers separation achieved on S-amlodipine-imprinted capillary was up to 16.1. The strong recognition ability (selectivity factor was 3.23) and high column performance (theory plates was 26,053 plates m(-1)) of template were obtained. The MIP coatings were also prepared using either S-naproxen or S-ketoprofen as template molecule. The resolutions of enantiomers separation were 2.20 and 4.56, respectively. The results illustrate that the synthesis of MIP using one monomer is not only an experimental-simplified process, but also an approach to producing chiral stationary phase with high efficiency and selectivity.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Ze-Hui
authors.initials: ZH
authors.lastname: Wei
authors.forename: Xi
authors.initials: X
authors.lastname: Wu
authors.forename: Bian
authors.initials: B
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Huang
authors.forename: Zhao-Sheng
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D017311:Amlodipine / Q000737:chemistry / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007660:Ketoprofen / Q000737:chemistry / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009288:Naproxen / Q000737:chemistry / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D011108:Polymers; D017311:Amlodipine; D009288:Naproxen; D007660:Ketoprofen