title: Characterization of a chalcone synthase (CHS) flower-specific promoter from Lilium orential 'Sorbonne'.
abstract: The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS promoter was fused to the β-glucuronidase (GUS) gene to characterize its spatial and temporal expression in Petunia                         hybrida 'Dreams Midnight' using an Agrobacterium-mediated leaf disc transformation method. Our results demonstrated that GUS expression was present in flowers, but reduced or absent in the other tissues (leaf and stem) examined. In petals, GUS activity reached its peak at flower developmental stage 4, and decreased at later stages. Deletion analysis indicated that even a 307-bp fragment of the PLoCHS promoter could still direct flower-specific expression. Further deletion of the region from -261 to -72 bp resulted in weak expression in different organs, including flowers, leaves and stems. This evidence combined with prediction of cis-acting elements in the PLoCHS promoter suggests that the TACPyAT box located in this promoter plays a key role in the regulation of organ-specific expression.
authors.affiliation: College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China. lyl6151@126.com
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Lou
authors.forename: Weirong
authors.initials: W
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
authors.forename: Carole
authors.initials: C
authors.lastname: Bassett
authors.forename: Yuejin
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D035264:Flowers / Q000201:enzymology* / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D027762:Lilium / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D032306:Petunia / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D014170:Transformation, Genetic
chemical_list: D010940:Plant Proteins; D000217:Acyltransferases; C020141:flavanone synthetase