title: Nanoparticle-based biocompatible and targeted drug delivery: characterization and in vitro studies.
abstract: Paclitaxel nanoparticles (PAX NPs) prepared with the size of 110 ± 10 nm and ζ potential of -40 ± 3 mV were encapsulated in synthetic/biomacromolecule shell chitosan, dextran-sulfate using a layer-by-layer self-assembly technique. Zeta potential measurements, analysis of X-ray photoelectron spectroscopy, and scanning electron microscopy confirmed the successful adsorption of each layer. Surface modifications of these core-shell NPs were performed by covalently conjugating with poly(ethylene glycol) (H(2)N-PEG-carboxymethyl, M(w) 3400) and fluorescence labeled wheat germ agglutinin (F-WGA) to build a biocompatible and targeted drug delivery system. 32% of PAX was released from four bilayers of biomacromolecule assembled NPs within 8 h as compared with >85% of the drug released from the bare NPs. Moreover, high cell viability with PEG conjugation and high binding capacity of WGA-modified NPs with Caco-2 cells were observed. This biocompatible and targeted NP-based drug delivery system, therefore, may be considered as a potential candidate for the treatment of colonic cancer and other diseases.
authors.affiliation: Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, United States.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.forename: Michael V
authors.initials: MV
authors.lastname: Pishko
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D016264:Dextran Sulfate / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D004339:Drug Compounding / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D058990:Molecular Targeted Therapy / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D017239:Paclitaxel / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry; D013499:Surface Properties; D014909:Wheat Germ Agglutinins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D001672:Biocompatible Materials; D004337:Drug Carriers; D014909:Wheat Germ Agglutinins; D011092:Polyethylene Glycols; D048271:Chitosan; D016264:Dextran Sulfate; D017239:Paclitaxel