title: Lindane stimulates neutrophils by selectively activating phospholipase C and phosphoinositide-kinase activity.
abstract: The organochlorine insecticide lindane is a known activator of neutrophil responses. In this work we delineated the biochemical pathways by which lindane stimulates neutrophil oxidant production. Plasma membrane GTPase activity was not stimulated by lindane, ruling out a role for lindane-induced activation of G-proteins or G-protein coupled receptors, whereas inhibition of phospholipase C inhibited lindane-induced oxidant production. Together these data pointed to phospholipase C as the direct target of lindane activation. Type I phosphoinositide 3-kinase was not significantly activated by lindane and an inhibitor of phosphoinositide 3-kinases inhibited oxidant production by only 40%. Thus, Type I phosphoinositide 3-kinase played a minor role, if any, in lindane-induced oxidant production. Lindane stimulated an increase in phosphatidylinositol phosphate suggesting a Type II or III phosphotidylinositol 3-kinase or phosphatidylinositol 4-kinase activity was also stimulated.
authors.affiliation: Departments of Surgery and Biological Chemistry, University of Michigan Medical Center and the Research Service (11R), VA Medical Center, 2215 Fuller Road, Ann Arbor, MI 48105, USA.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Rebbapragada
authors.forename: Michael L
authors.initials: ML
authors.lastname: Keil
authors.forename: Naveen L
authors.initials: NL
authors.lastname: Solomon
authors.forename: Alexis
authors.initials: A
authors.lastname: Traynor-Kaplan
authors.forename: Geneva M
authors.initials: GM
authors.lastname: Omann