title: Metastasis gene expression analyses of choriocarcinoma and the effect of silencing metastasis-associated genes on metastatic ability of choriocarcinoma cells.
abstract: OBJECTIVE
  Obtaining choriocarcinoma metastasis-associated genes and identifying the role and mechanism of VEGF-B in the progression of human choriocarcinoma.
  
  
  METHODS
  (1) cDNA microarray technique was used to compare the transcriptional profiles between highly metastatic JEG-3 cells and lowly metastatic JAR cells; (2) An inhibitory effect of VEGF-B shRNA was demonstrated by RT-PCR; (3) The effect of VEGF-B shRNA on invasion of JEG-3 cells in vitro was detected by Matrigel invasion assay.
  
  
  RESULTS
  (1) In upregulated genes, 51 genes were correlated with the cell metastasis ability, and FN, MMP-2, uPA, CAV-1 and VEGF-B were the first five genes; (2) Afterwards transfected VEGF-B shRNA, VEGF-B mRNA expression decreased obviously; (3) VEGF-B shRNA transfection significantly downregulated invasion level of JEG-3 cells in vitro (p < 0.05).
  
  
  CONCLUSIONS
  VEGF-B plays an important role in the metastatic capability of human choriocarcinoma. Reducing the expression of VEGF-B can help weaken the invasion ability of human choriocarcinoma.
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: L
authors.initials: L
authors.lastname: Huining
authors.forename: C
authors.initials: C
authors.lastname: Jingting
authors.forename: H
authors.initials: H
authors.lastname: Keren
mesh_terms: D045744:Cell Line, Tumor; D002822:Choriocarcinoma / Q000235:genetics* / Q000473:pathology / Q000556:secondary*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042561:Vascular Endothelial Growth Factor B / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D042561:Vascular Endothelial Growth Factor B