title: [Relation between gastric dendritic cells and H pylori-associated gastritis].
abstract: OBJECTIVE
  To investigate the effects of gastric dendritic cells(DCs) in H.pylori-associated gastritis.
  
  
  METHODS
  Mononuclear cells were isolated from gastric samples of endoscoped healthy subjects or patients with gastritis by Ficoll-hypaquetechnique. HLA-DR(+);DCs were further isolated by MACS. Rapid urease test, warthin-starry dyer and C(14);-urea breath test were used to detect H.pylori. The relationship between the quantity of gastric DCs and H.pylori density or inflammatory background were analysed.
  
  
  RESULTS
  The gastric mononuclear cells from H.pylori-infected normal gastric mucosa contained more HLA-DR(+);DCs (17.93 % ) than that from noninfected subjects (4.93%).The quantity of gastric DCs is positively correlated with H.pylori density. The quantity of gastric DCs were significantly higher in moderate and high inflammatory gastric mucosa than that in low inflammatory gastric mucosa. There was no difference in the quantity of gastric DCs between moderate and high inflammatory gastric mucosa.
  
  
  CONCLUSIONS
  Successfully isolated gastric DCs. DCs may play a role in the early stage of H.pylori-associated gastritis.
authors.affiliation: Department of Digestive Deseases, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Han-bing
authors.initials: HB
authors.lastname: Ning
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Lian-feng
authors.initials: LF
authors.lastname: Zhang
authors.forename: Ji-chang
authors.initials: JC
authors.lastname: Li
mesh_terms: D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology; D005756:Gastritis / Q000276:immunology* / Q000382:microbiology*; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged