title: Polypeptide aptamer selection using a stabilized ribosome display.
abstract: A newly developed ribosome display protocol was applied to the in vitro selection of polypeptide aptamers to small molecular weight chemicals, 6-[hydroxy(4-nitrobenzyl)phosphonyl]hexanoic acid and vitamin B12, chosen from a peptide library of random sequences. New peptide sequences binding to the targets were found after six rounds of this protocol.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Shuta
authors.initials: S
authors.lastname: Hara
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Liu
authors.forename: Toshiro
authors.initials: T
authors.lastname: Aigaki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Shimizu
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry* / Q000378:metabolism; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D009841:Oligonucleotides / Q000378:metabolism; D019151:Peptide Library; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D052158:Aptamers, Peptide; D009841:Oligonucleotides; D019151:Peptide Library