title: Monolithic poly(butyl methacrylate-ethylene dimethacrylate-methacrylic acid) column for capillary electrochromatography.
abstract: A butyl methacrylate-ethylene dimethacrylate-methacrylic acid (MAA) monolithic column was prepared for capillary electrochromatography. The effect of MAA weight percentage on the EOF mobility and column efficiency was studied. BMA-EDMA-MAA monolith with higher content of MAA provided higher EOF mobility as well as higher efficiency. The effect of mobile-phase composition and buffer pH was also investigated. The monolithic columns exhibited a good repeatability and reproducibility of column preparation with relative standard deviation values below 16% in the studied chromatographic parameters.
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan; Nagasaki University Research Centre for Genomic Instability and Carcinogenesis (NRGIC), Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Daisuke
authors.initials: D
authors.lastname: Horiguchi
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation*; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; C004919:ethylene dimethacrylate; C016284:butyl methacrylate