title: Anticonvulsant, antidepressant-like activity of Abelmoschus manihot ethanol extract and its potential active components in vivo.
abstract: Depression is the most common psychiatric comorbidity in patients with epilepsy. Searching for antiepileptic (anticonvulsant) and antidepressant-like medicines from natural products is very important for the treatment of this disease. The flower of Abelmoschus manihot (Linn.) Medicus has been reported to have neuroprotective effect against cerebral ischemia injury. In order to further explore the activity of Abelmoschus manihot on the central nervous system, the anticonvulsant and antidepressant-like effects of Abelmoschus manihot ethanol extract (AMEE) as well as its potential active components in vivo was investigated in the present study. It was found that AMEE could protect mice against PTZ-induced clonic convulsions and mortality. AMEE could also decrease immobility time in the FST in mice. Furthermore, the potential active components of AMEE in rat brain were identified by ultra performance liquid chromatography-mass spectrometer (UPLC-MS). Five parent components including isoquercitrin, hyperoside, hibifolin, quercetin-3'-O-glucoside, quercetin and three metabolites were detected in rat brain after administration of AMEE. In conclusion, eight flavonoids were identified in rat brain after administration of AMEE; meanwhile, these flavonoids might represent the potential bioactive components of AMEE and contribute to its anticonvulsant and antidepressant-like activity in vivo.
authors.affiliation: Jiangsu Key Laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing, PR China.
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Caifu
authors.initials: C
authors.lastname: Xue
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: You
mesh_terms: D028483:Abelmoschus / Q000737:chemistry*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D000928:Antidepressive Agents / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D004341:Drug Evaluation; D000431:Ethanol / Q000737:chemistry; D005080:Exercise Test; D005419:Flavonoids / Q000378:metabolism; D035264:Flowers / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010433:Pentylenetetrazole / Q000009:adverse effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy; D013997:Time Factors
chemical_list: D000927:Anticonvulsants; D000928:Antidepressive Agents; D005419:Flavonoids; D010936:Plant Extracts; C016527:isoquercitrin; D000431:Ethanol; C087346:hibifolin; C021304:hyperoside; D011794:Quercetin; D010433:Pentylenetetrazole