title: Putative proto-oncogene Pir expression is significantly up-regulated in the spleen and kidney of cytosolic superoxide dismutase-deficient mice.
abstract: Iron binding protein pirin was isolated as an interactor of the NFIX transcription factor but it can also form complexes with Bcl3 and NF-κB1(p50). Alterations of pirin expression were observed in various tumors and after exposure to pro-carcinogenic oxidative stressors. The aim of the present work was to study the level of pirin transcription in an in vivo model of oxidative stress, namely, in Sod1-deficient mice. We have found that Sod1(-/-) mice have a significantly elevated level of Pir mRNA in the spleen and kidney but not in the liver, heart, or/and brain. We have also shown that similarly to its human ortholog, the mouse Pir gene transcription level varies significantly between organs. The highest expression was found in the liver and the lowest in the spleen and kidney. Based on literature data, we propose the involvement of Nrf2, AP-1, and NF-κB transcription factors in Pir up-regulation in Sod1(-/-) mice.
authors.affiliation: Centre for Radiobiology and Biological Dosimetry, Warsaw, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Brzóska
authors.forename: Tomasz M
authors.initials: TM
authors.lastname: Stępkowski
authors.forename: Marcin
authors.initials: M
authors.lastname: Kruszewski
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D049308:Dioxygenases; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D018345:Mice, Knockout; D023421:Models, Animal; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism; D051839:NFI Transcription Factors / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; C000715470:MAS1 protein, human; D052002:NF-kappa B p50 Subunit; D051839:NFI Transcription Factors; C497650:Nfix protein, mouse; D009687:Nuclear Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D049308:Dioxygenases; C498418:Pir protein, mouse; D013482:Superoxide Dismutase