title: Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains.
abstract: A comparative proteomic approach, using two dimensional gel electrophoresis and mass spectrometry, has been developed to compare and elucidate the differences among the cellular proteomes of four closely related isogenic O/C, SIN, N/R and T, B. clausii strains during both exponential and stationary phases of growth. Image analysis of the electropherograms reveals a high degree of concordance among the four proteomes, some proteins result, however, differently expressed. The proteins spots exhibiting high different expression level were identified, by mass-spectrometry analysis, as alcohol dehydrogenase (ADHA, EC1.2.1.3; ABC0046 isoform) aldehyde dehydrogenase (DHAS, EC 1.2.1.3; ABC0047 isoform) and flagellin-protein of B. clausii KSM-k16. The different expression levels of the two dehydrogenases were confirmed by quantitative RT-PCR and dehydrogenases enzymatic activity. The different patterns of protein expression can be considered as cell proteome signatures of the different strains.
authors.affiliation: Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, (CNR) Bari, Italy. r.lippolis@ibbe.cnr.it
authors.forename: Rosa
authors.initials: R
authors.lastname: Lippolis
authors.forename: Antonio
authors.initials: A
authors.lastname: Gnoni
authors.forename: Anna
authors.initials: A
authors.lastname: Abbrescia
authors.forename: Damiano
authors.initials: D
authors.lastname: Panelli
authors.forename: Stefania
authors.initials: S
authors.lastname: Maiorano
authors.forename: Maria Stefania
authors.initials: MS
authors.lastname: Paternoster
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Sardanelli
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
authors.forename: Antonio
authors.initials: A
authors.lastname: Gaballo
mesh_terms: D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome