title: [Expression of PTX3 gene in Caco-2 cells treated with Lactobacillus acidophilus NCFM].
abstract: OBJECTIVE
  To study the expression of immunity and inflammatory mediator factor PTX3 in intestinal epithelial cells treated with Lactobacillus acidophilus NCFM and further to reveal the regulatory mechanism.
  
  
  METHODS
  Caco-2 cells were cocultured with Lactobacillus acidophilus NCFM for 0, 2, 4, 8, 12 h and 0, 0.5, 1, 2, 4 h respectively, then the total RNA and protein were extracted. The expression of PTX3 gene was analyzed by Real Time RT-PCR. The phosphorylation levels of NF-kappaB was analyzed by Western Blot. Caco-2 cells were pretreated with PDTC for 30 min before cocultured with Lactobacillus acidophilus NCFM for 2 h, then the total RNA was extracted and the expression of PTX3 gene was analyzed by Real Time RT-PCR.
  
  
  RESULTS
  Lactobacillus acidophilus NCFM could induce the expression of PTX3 in Caco-2 cells. The PTX3 expression peaked at 4 h after coculture. Then its expression gradually waned out. Lactobacillus acidophilus NCFM could rapidly activate the phosphorylation of NF-kappaB, and the expression of PTX3 was decreased notably after pretreated with PDTC for 30 min.
  
  
  CONCLUSIONS
  Lactobacillus acidophilus NCFM could transiently regulate the immunity and inflammatory mediator factor PTX3 expression through rapidly activating NF-kappaB signaling pathway in Caco-2 cells.
authors.affiliation: Key Lab of Dairy Science, Ministry of Education, College of Food Science and Engineering, Northeast Agricultural University, Harbin 150030, China. 361725510@qq.com
authors.forename: Xuena
authors.initials: X
authors.lastname: Lv
authors.forename: Chaoxin
authors.initials: C
authors.lastname: Man
authors.forename: Linlin
authors.initials: L
authors.lastname: Han
authors.forename: Mingna
authors.initials: M
authors.lastname: Wang
authors.forename: Guanghui
authors.initials: G
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Shiqin
authors.initials: S
authors.lastname: Yang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Xue
authors.forename: Yujun
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D002097:C-Reactive Protein / Q000235:genetics* / Q000276:immunology; D018938:Caco-2 Cells; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D015870:Gene Expression; D006801:Humans; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D007779:Lactobacillus acidophilus / Q000502:physiology*; D008954:Models, Biological; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D000683:Serum Amyloid P-Component / Q000235:genetics* / Q000276:immunology; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D016328:NF-kappa B; D000683:Serum Amyloid P-Component; D000099153:Pentraxins