title: Effects of dietary methylmercury on zebrafish skeletal muscle fibres.
abstract: Mercury and more specifically methylmercury have been reported as hazardous environmental pollutants able to accumulate along the aquatic food chain with severe risk for animal and human health. Adult male zebrafish (Danio rerio) were distributed in two groups: a control group (fed with uncontaminated food) and a MeHg-contaminated group (fed with food containing 13.5μgHgg(-1) (dry wt)). Five fish per condition were removed after 7, 21 and 63 days. Bioaccumulation of mercury was determined and muscle samples from control and exposed groups were fixed for histological and ultrastructural studies. In contaminated muscles were observed a decrease of the inter-bundle surface, mitochondria with variable shapes, sizes and cristae disorganization, also decreasing the surface area and inter-bundle surfaces. Indeed, damage in the endoplasmic reticulum cisternae was observed. For statistical evaluation the damages in mitochondria was quantified by image. According to the current results, methylmercury affects the structure of fibre cells of D. rerio after trophic and low dose exposure.
authors.affiliation: Laboratorio de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Parana, CP 19031, CEP:81.531-990, Curitiba, PR, Brazil.
authors.forename: Ciro Alberto
authors.initials: CA
authors.lastname: de Oliveira Ribeiro
authors.forename: Mesmer-Dudons
authors.initials: MD
authors.lastname: Nathalie
authors.forename: Patrice
authors.initials: P
authors.lastname: Gonzalez
authors.forename: Dominique
authors.initials: D
authors.lastname: Yannick
authors.forename: Bourdineaud
authors.initials: B
authors.lastname: Jean-Paul
authors.forename: Alain
authors.initials: A
authors.lastname: Boudou
authors.forename: Jean Charles
authors.initials: JC
authors.lastname: Massabuau