title: MicroRNA-22 and microRNA-140 suppress NF-κB activity by regulating the expression of NF-κB coactivators.
abstract: Nuclear factor κB (NF-κB) is a transcription factor that regulates a set of genes that are critical to many biological phenomena, including liver tumorigenesis. To identify microRNAs (miRNAs) that regulate NF-κB activity in the liver, we screened 60 miRNAs expressed in hepatocytes for their ability to modulate NF-κB activity. We found that miRNA-22 and miRNA-140-3p significantly suppressed NF-κB activity by regulating the expression of nuclear receptor coactivator 1 (NCOA1) and nuclear receptor-interacting protein 1 (NRIP1), both of which are NF-κB coactivators. Our results provide new information about the roles of miRNAs in the regulation of NF-κB activity.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Takata
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Otsuka
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kojima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kishikawa
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Yoshida
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Koike
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics*; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics*; D000078723:Nuclear Receptor Interacting Protein 1; D011401:Promoter Regions, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C548375:MIRN22 microRNA, human; D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D016328:NF-kappa B; C000627127:NRIP1 protein, human; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1; D008156:Luciferases; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1