title: Spectrophotometric estimation of betahistine hydrochloride in tablet formulations.
abstract: OBJECTIVE
  The study aims to develop simple, sensitive, rapid, accurate and precise spectrophotometric method for estimation of Betahistine hydrochloride in tablet dosage forms.
  
  
  METHODS
  For method I and II, in a series of 10 ml volumetric flask, aliquots of standard drug solution (100 μg/ml) in double distilled water were transferred and diluted with same so as to give several dilutions in concentration range of 15-90 μg/ml and 10-80 μg/ml respectively of betahistine hydrochloride. To 5 ml of each dilution taken in a separating funnel, (5 ml of methyl orange for method I and 5 ml of bromo phenol blue for method II) reagent and 5 ml of chloroform was added. Reaction mixture was shaken gently for 5 min and allowed to stand so as to separate aqueous and chloroform layer. Absorbance maxima measured at 421.6 nm and 412 nm for method I and II respectively.
  
  
  RESULTS
  The recovery studies were found close to 100 % that indicates accuracy and precision of the proposed methods. The statistical analysis was carried out and results of which were found satisfactory. Standard deviation values were found low that indicated reproducibility of the proposed methods.
  
  
  CONCLUSIONS
  Based on results the developed methods could be used for routine estimation of betahistine hydrochloride from tablet formulations.
authors.affiliation: Department of Pharmaceutical Analysis, Swami Keshvanand Institute of Pharmacy, Bikaner, Rajasthan, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.forename: Sanju
authors.initials: S
authors.lastname: Nanda
authors.forename: Rajiv
authors.initials: R
authors.lastname: Chomwal