title: Microsatellite-based genotyping of Candida albicans isolated from patients with superficial candidiasis.
abstract: This study aimed to examine the genotype distribution of Candida albicans and the major genotypes involved in superficial candidiasis. The genotypes of C. albicans isolated from the infection sites of patients with superficial candidiasis (referred to as infection isolates) were analyzed by fragment analysis using 4 microsatellite markers (HIS3, CDC3, CAI and CAIII). Genotypes of the infection isolates were compared with those of C. albicans isolated from oral mucosa of non-candidiasis patients (referred to as oral isolates). Isolates of C. albicans showed 4 major genotypes for HIS3/CAI (" a " for 148 : 148 / 23 : 23," b " for 148 : 160 / 33 : 41," c " for 148 : 164 / 32 : 41 and " d " for 152 : 152 / 18 : 27). The genotypes " a "," b " and " d " were commonly found in oral (4.7, 8.8 and 7.6%, respectively) and infection (6.6, 9.2 and 15.4%, respectively) isolates. No isolates of genotype " c " were isolated from infection sites. The genotype " a " was found in the isolates from patients with genitalia candidiasis. Genotyping of multiple isolates from an individual patient showed that C. albicans from infection sites was genetically homogenous as compared with that of oral isolates, even in the same patient with candidiasis.
authors.affiliation: Department of Dermatology, Nagoya University Graduate School of Medicine, Japan.
authors.forename: Kazue
authors.initials: K
authors.lastname: Shimizu
authors.forename: Hisao
authors.initials: H
authors.lastname: Hattori
authors.forename: Hidesada
authors.initials: H
authors.lastname: Adachi
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Oshima
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Horii
authors.forename: Reiko
authors.initials: R
authors.lastname: Tanaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Yaguchi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tomita
authors.forename: Masashi
authors.initials: M
authors.lastname: Akiyama
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kawamoto
authors.forename: Toshio
authors.initials: T
authors.lastname: Kanbe
mesh_terms: D000328:Adult; D000368:Aged; D002176:Candida albicans / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology*; D002179:Candidiasis, Cutaneous / Q000382:microbiology; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged