title: [Heat coagulation of middle meningeal artery affects plasma CGRP and substance P levels in migraine rat triggered by nitroglycerin].
abstract: OBJECTIVE
  To detect the influence of heat coagulation of middle meningeal artery (MMA) on plasma CGRP and SP levels in migraine rat triggered by nitroglycerin (NTG).
  
  
  METHODS
  Twenty-four female Wistar rats were randomly divided into four groups, with six rats in each group. In group A, rats were treated with isotonic saline; in group B, rats were injected subcutaneously with 10 mg/kg nitroglycerin to set up the animal model of migraine; in group C, rats were subjected to sham surgery by merely exposing MMA and then injected with nitroglycerin (10 mg/kg); and in group D, rats were firstly subjected to heat coagulation of MMA and, subsequently, injected with nitroglycerin (10 mg/kg). Following the injection, the animal's behavior was continuously recorded by an observer. Radioimmunoassay was employed to measure the amounts of CGRP and SP in venous plasma.
  
  
  RESULTS
  Injection of 10 mg/kg nitroglycerin was able to trigger the animal model of migraine as evidenced by the appearances of a series of symptoms, such as, scratching head frequently, climbing cage and red ear. Rats in group A occasionally showed off scratching head, whereas, rats from group B and group C presented with much severe symptoms. Of note, rats in group D appeared slightly scratching head and climbing cage, without red ear compared with group B and C. At the same time, the amounts of plasma CGRP and SP in group B, group C, or group D were increased significantly compared with that of group A (P < 0.05). Furthermore, the plasma CGRP and SP levels in group D were much lower than that in group B and group C (P < 0.05), but there was no statistical difference between group B and group C (P > 0.05).
  
  
  CONCLUSIONS
  Heat coagulation of MMA may relieve symptoms of rats following nitroglycerin infusion, possibly by inhibiting the release of CGRP and SP.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Ji'nan, 250012, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhu
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Han
authors.forename: Wenping
authors.initials: W
authors.lastname: Xiong
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Fan
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000097:blood* / Q000378:metabolism; D004564:Electrocoagulation; D005260:Female; D008576:Meningeal Arteries; D008881:Migraine Disorders / Q000139:chemically induced / Q000378:metabolism* / Q000628:therapy; D005996:Nitroglycerin / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000097:blood* / Q000378:metabolism
chemical_list: D013373:Substance P; D005996:Nitroglycerin; D015740:Calcitonin Gene-Related Peptide