title: BjMT2, a metallothionein type-2 from Brassica juncea, may effectively remove excess lead from erythrocytes and kidneys of rats.
abstract: The remedial effects of a plant metallothionein type-2 were observed from lead (Pb) injured rats. BjMT2 from Brassica juncea was expressed in Escherichia coli and purified by affinity chromatography, a purified BjMT2 protein was obtained which strongly reacted with the thiol reagent MBB (monobromobimane). The profiles of erythrocytes, renal tubules and glomerulus of kidney of rats suffered pathological changes from excess Pb were evidently improved by supplying the BjMT2. Quantitative analysis showed that the content of Pb and the amount of leukocytes in blood were significantly declined after supplying BjMT2 to rats. The results indicated that the BjMT2 may have the potential function to decrease Pb toxicification in rate organs and tissues.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China; College of Basic Medicine, Jiamusi University, Jiamusi 154007, PR China.
authors.forename: Jun-Xing
authors.initials: JX
authors.lastname: Liu
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Shi
authors.forename: Yu-Zhuo
authors.initials: YZ
authors.lastname: Ai
authors.forename: Ye-Jie
authors.initials: YJ
authors.lastname: Du
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: An