title: Bovine serum albumin: survival and osmolarity effect in bovine spermatozoa stored above freezing point.
abstract: Liquid nitrogen preservation in remote farms is a limitation. The goal of this study was to determine optimum temperature above freezing point for bovine spermatozoa preservation using bovine serum albumin (BSA) as a supplementation. Pooled semen sample from three ejaculates was subjected to various BSA concentration (1, 4, 8 and 12 mg ml(-1)), before incubation in different above freezing point temperatures (4, 25 and 37 °C). Viability assessment was carried out against time from day 0 (fresh sample) until all spermatozoa become nonviable. Optimal condition for bovine spermatozoa storage was at 4 °C with 1 mg ml(-1) BSA for almost 7 days. BSA improved bovine spermatozoa viability declining rate to 44.28% at day 4 and 57.59% at day 7 compared to control, with 80.54% and 98.57% at day 4 and 7 respectively. Increase in BSA concentration did not improve sperm viability. Our results also confirmed that there was a strong negative correlation between media osmolarity and bovine spermatozoa survival rate with r = 0.885, P < 0.0001. Bovine serum albumin helps to improve survival rate of bovine spermatozoa stored above freezing point.
authors.affiliation: Department of Biomedical Sciences, Faculty of Allied Health Sciences, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
authors.forename: C F
authors.initials: CF
authors.lastname: Nang
authors.forename: K
authors.initials: K
authors.lastname: Osman
authors.forename: S B
authors.initials: SB
authors.lastname: Budin
authors.forename: M I
authors.initials: MI
authors.lastname: Ismail
authors.forename: F H F
authors.initials: FH
authors.lastname: Jaffar
authors.forename: S F S
authors.initials: SF
authors.lastname: Mohamad
authors.forename: S F
authors.initials: SF
authors.lastname: Ibrahim
mesh_terms: D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D005615:Freezing; D008297:Male; D009994:Osmolar Concentration; D012662:Semen Preservation; D012710:Serum Albumin, Bovine / Q000502:physiology*; D013094:Spermatozoa
chemical_list: D012710:Serum Albumin, Bovine