title: The interaction between ubiquitin C-terminal hydrolase 37 and glucose-regulated protein 78 in hepatocellular carcinoma.
abstract: The ubiquitin C-terminal hydrolase (UCH) is a subfamily of deubiquitinating enzymes, which consists of four members: UCH-L1, UCH-L3, UCH37, and BRCA1-associated protein-1. Although there is growing evidence that UCH enzymes and human malignancies are closely correlated, there have been few studies on UCH37, especially on its interactions with other proteins. In the current study, a functional proteomic analysis was performed to screen UCH37-interacting proteins in hepatocellular carcinoma (HCC), and glucose-regulated protein 78 was identified as one interacting with UCH37, which was confirmed by co-immunoprecipitation and confocal laser scanning microscopy analysis, suggesting that their interaction could provide a new insight into the mechanism of HCC.
authors.affiliation: The Department of Gastroenterology of Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai, 200032, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.forename: Jingui
authors.initials: J
authors.lastname: Mu
authors.forename: Yushui
authors.initials: Y
authors.lastname: Ma
authors.forename: Duan
authors.initials: D
authors.lastname: Ma
authors.forename: Da
authors.initials: D
authors.lastname: Fu
authors.forename: Xizhong
authors.initials: X
authors.lastname: Shen
mesh_terms: D002268:Carboxypeptidases / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D025941:Protein Interaction Mapping; D040901:Proteomics; D043222:Ubiquitin Thiolesterase / Q000378:metabolism*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; C018551:glucose-regulated proteins; D002268:Carboxypeptidases; C421245:UCHL5 protein, human; D043222:Ubiquitin Thiolesterase