title: Regulation of ciliary beat frequency by the nitric oxide signaling pathway in mouse nasal and tracheal epithelial cells.
abstract: OBJECTIVE
  Our purpose was to investigate the role of the nitric oxide (NO) signaling pathway in the regulation of ciliary beat frequency (CBF) in mouse nasal and tracheal epithelial cells.
  
  
  METHODS
  We studied the effects of the NO donor l-arginine (L-Arg) and specific inhibitors of the NO signaling pathway on CBF of both nasal and tracheal epithelial cells by using high-speed digital microscopy. We also examined eNOS, sGC β, PKG I and acetylated α tubulin expression in native mouse nasal and tracheal epithelium using immunohistochemical methods.
  
  
  RESULTS
  L-Arg significantly increased CBF of cultured nasal and tracheal epithelial cells, and the effects were blocked by pretreatment with N(G)-nitro-l-arginine methyl ester (L-NAME), a NOS inhibitor, with LY-83583, a sGC inhibitor, or with KT-5823, a PKG inhibitor. Positive immunostaining for NO signaling molecules including eNOS, sGC β and PKG I was observed in either nasal or tracheal ciliated epithelium.
  
  
  CONCLUSIONS
  NO plays a role in regulating CBF of mouse respiratory epithelial cells via a eNOS-NO-sGC β-cGMP-PKG I pathway.
authors.affiliation: Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education), Beijing Institute of Otolaryngology, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jiao
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Lou
authors.forename: Shanzhe
authors.initials: S
authors.lastname: Jin
authors.forename: Erzhong
authors.initials: E
authors.lastname: Fan
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Demin
authors.initials: D
authors.lastname: Han
authors.forename: Luo
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009297:Nasal Mucosa / Q000166:cytology*; D009569:Nitric Oxide / Q000378:metabolism*; D015398:Signal Transduction; D014132:Trachea / Q000166:cytology*
chemical_list: D009569:Nitric Oxide