title: Effect of the exposure to metal lead on the regenerative ability of Lumbriculus variegatus (Oligochaeta).
abstract: Lumbriculus variegatus is a recommended species for use in sediment toxicity tests and is known to have a remarkable power of segmental regeneration. Here, we tested the effects of a chemical stressor on the regenerative ability of L. variegatus and investigated the potential of regenerative ability as an additional new parameter in standard toxicity tests. The worms were cut into two equal segments, and exposed to various concentrations of lead. Two assays were performed: one with sediment spiked with lead and the other with water spiked with lead. The endpoints were segmental regeneration, survival and behaviour. Regenerative ability was clearly affected by exposure to lead-contaminated sediment and lead-contaminated water. Organisms exposed to lead grew more slowly than those not exposed; worms exposed to contaminated water showed higher mortalities than those exposed to contaminated sediment. Results showed that L. variegatus' regenerative ability, as a developmental test parameter, is more sensitive than mortality.
authors.affiliation: CESAM & Departamento de Biologia, Universidade de Aveiro, Portugal. amsardo@ua.pt
authors.forename: Ana Margarida
authors.initials: AM
authors.lastname: Sardo
authors.forename: Lourdes
authors.initials: L
authors.lastname: Pereira
authors.forename: Almut
authors.initials: A
authors.lastname: Gerhardt
authors.forename: Amadeu M V M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods; D019015:Geologic Sediments / Q000737:chemistry; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity*; D009835:Oligochaeta / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects*; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; D007854:Lead