title: Platelet activation in chronic cocaine users: effect of short term abstinence.
abstract: Cocaine abuse increases the risk of cardiac and cerebrovascular events, such as myocardial infarction and ischemic stroke. The underlying mechanisms leading to these complications are not fully understood although intravascular thrombus formation has been observed. The aim of this study was to investigate the existence of platelet activation and the effect of short-term abstinence in chronic cocaine consumers. We studied 23 cocaine dependent individuals (aged 20-54 years) who met DSM-IV criteria for cocaine dependence and 20 controls. Samples were obtained at baseline, within 72 h of last drug exposure and after 4 weeks of controlled abstinence. Monocyte-platelet aggregates (MPA) were measured by flow cytometry. Plasma levels of soluble CD40L (sCD40L), Neutrophil-Activating Peptide-2 (NAP-2) and regulated on activation normal T cells expressed and secreted (RANTES) were determined by ELISA. Levels of MPA, sCD40L, NAP-2 and RANTES were significantly higher (all p < 0.05) in cocaine addicts compared to controls at baseline. All the parameters returned to values similar to the control group after 4-weeks' abstinence. Levels of sCD40L and RANTES were associated with an index of intensity of drug consumption (p < 0.02). Our results demonstrate that cocaine use induces platelet activation which is a prominent finding after recent consumption. The persistence over time of this condition may contribute not only to acute thrombotic complications but also to the development of early-onset atherosclerotic process observed in cocaine abusers.
authors.affiliation: Department of Hematology-Oncology, School of Medicine, Pontificia Universidad Católica de Chile. jpereira@med.puc.cl
authors.forename: Jaime
authors.initials: J
authors.lastname: Pereira
authors.forename: Claudia G
authors.initials: CG
authors.lastname: Sáez
authors.forename: Julio
authors.initials: J
authors.lastname: Pallavicini
authors.forename: Olga
authors.initials: O
authors.lastname: Panes
authors.forename: Karla
authors.initials: K
authors.lastname: Pereira-Flores
authors.forename: Manuel J
authors.initials: MJ
authors.lastname: Cabreras
authors.forename: Teresa
authors.initials: T
authors.lastname: Massardo
authors.forename: Diego
authors.initials: D
authors.lastname: Mezzano
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D023201:CD40 Ligand / Q000097:blood; D002449:Cell Aggregation / Q000187:drug effects; D003042:Cocaine / Q000494:pharmacology*; D019970:Cocaine-Related Disorders / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D055815:Young Adult; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D015415:Biomarkers; C061615:PPBP protein, human; D001620:beta-Thromboglobulin; D023201:CD40 Ligand; D003042:Cocaine