title: Enhancing the utility of a prM/E-expressing chimeric vaccine for Japanese encephalitis by addition of the JEV NS1 gene.
abstract: Recently, we demonstrated that a single-cycle West Nile virus (WNV) named RepliVAX WN could be used to produce a chimeric Japanese encephalitis (JE) vaccine (RepliVAX JE) by replacing the WNV prM/E genes with those of JEV. Here, we tested if replacement of WNV NS1 gene in RepliVAX JE with that of JEV (producing TripliVAX JE) could produce a superior vaccine. TripliVAX JE elicited higher anti-E immunity and displayed better efficacy in mice than RepliVAX JE. Furthermore, TripliVAX JE displayed reduced immune interference caused by pre-existing anti-NS1 immunity. Thus, we propose prM/E/NS1 chimerization as a new strategy for flavivirus vaccine development.
authors.affiliation: Department of Pathology, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0436, USA.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Gongbo
authors.initials: G
authors.lastname: Wang
authors.forename: Douglas G
authors.initials: DG
authors.lastname: Widman
authors.forename: Ernesto
authors.initials: E
authors.lastname: Infante
authors.forename: Evandro R
authors.initials: ER
authors.lastname: Winkelmann
authors.forename: Nigel
authors.initials: N
authors.lastname: Bourne
authors.forename: Peter W
authors.initials: PW
authors.lastname: Mason
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology* / Q000517:prevention & control; D022321:Japanese Encephalitis Vaccines / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D017423:Sequence Analysis, RNA; D014611:Vaccination; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D022321:Japanese Encephalitis Vaccines; C100315:NS1 protein, Flavivirus; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins