title: Overexpression of apoptosis-associated speck-like protein in P388D1 murine lymphoma cells affects metastatic properties.
abstract: Apoptosis-associated speck-like protein (ASC) is a bipartite adaptor molecule that participates in inflammation and apoptosis. ASC silencing has been observed in a significant proportion of human cancers. Here, we examined the role of ASC overexpression in the metastasis of the P388D1 murine lymphoma cell line to the liver, lung, spleen and kidney. First, we determined that the P388D1 cells express ASC. Then, ASC overexpression in P388D1 was achieved by transfecting pEGFP-ASC-C2 into the P388D1 cells. Furthermore, after the ASC-overexpressing P388D1 cells were injected into DBA/2 mice through the vena caudalis, their metastasis to the lung and the liver was significantly reduced in the pEGFP-ASC-C2-transfected group. These data indicate that ASC overexpression affects the in vivo metastatic properties of P388D1 cells.
authors.affiliation: Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Changwei
authors.initials: C
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Shuangyi
authors.initials: S
authors.lastname: Ren
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000502:physiology*; D007941:Leukemia P388 / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis; D014162:Transfection
chemical_list: D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D003598:Cytoskeletal Proteins; C498534:Pycard protein, mouse