title: [Disintegration of lactobacilli and enterococci for obtaining cell wall fragments].
abstract: The methods of Gram-positive cells disintegration to obtain cell wall fragments have been described. It has been shown that the use of ultrasonic disintegrator UZD-500 at the frequency 22 kHz during 30 min is optimal for disintegration of lactic acid bacteria cells. Also the ability to use grinding of cells in liquid nitrogen (6 times to the complete evaporation) for obtaining the cell wall fragments has been demonstrated.
authors.forename: O P
authors.initials: OP
authors.lastname: Livins'ka
authors.forename: N K
authors.initials: NK
authors.lastname: Kovalenko
authors.forename: I L
authors.initials: IL
authors.lastname: Harmasheva
mesh_terms: D002473:Cell Wall / Q000737:chemistry*; D016983:Enterococcus / Q000737:chemistry*; D007778:Lactobacillus / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods