title: Gene expression under laser and light-emitting diodes radiation for modulation of cell adhesion: Possible applications for biotechnology.
abstract: Experimental data about the modulation of adhesion and proliferation of anchorage-dependent HeLa cells with monochromatic or quasimonochromatic radiation in red to near-infrared region are presented. Cell adhesion and proliferation can be increased by irradiation with light of certain wavelengths (maxima in action spectrum are 619, 675, 740, 760, and 820 nm) or decreased when the activity of photoacceptor (cytochrome c oxidase in mitochondrial respiratory chain) is inhibited by chemicals before the irradiation. This modality allows controlling the number of attached and/or proliferating cells. Possible biotechnology applications of this method are outlined.
authors.affiliation: Laboratory of Laser Biology and Medicine, Institute of Laser and Information Technologies of Russian Academy of Sciences, Troitsk 142190, Moscow Region, Russian Federation. tkaru@isan.troitsk.ru
authors.forename: Tiina
authors.initials: T
authors.lastname: Karu
authors.forename: Ludmila
authors.initials: L
authors.lastname: Pyatibrat
mesh_terms: D001709:Biotechnology / Q000379:methods* / Q000639:trends; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology / Q000528:radiation effects*; D049109:Cell Proliferation / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D015398:Signal Transduction / Q000502:physiology*; D013057:Spectrum Analysis