title: An in silico DNA vaccine against Listeria monocytogenes.
abstract: Listeria monocytogenes causes listeriosis with mortality rate >20%. Listeriolysin-O (LLO), a pore-forming hemolysin, belongs to the family of cholesterol-dependent toxins (CDTX) and plays roles in the pathogenicity. In this study bioinformatic analyses were carried out on LLO sequence as a major immunodominant listerial antigen toward designing a DNA vaccine stimulating cytotoxic T-lymphocytes (CTLs). Mouse and human constructs were designed based on predicted T cell epitopes and MHC class I binders, which were then tandemly fused together. LLO-derived construct codons and a variety of critical gene expression efficiency parameters were optimized. Post-translational modifications such as glycosylation, phosphorylation were analysed. The constructs corresponded to LLO sequences of L. monocytogenes in BLAST search. Neither human nor mouse construct was allergen. Secretory pathway was location of the human construct that enhances immune induction and contribute to the efficacy of the vaccine candidate. mRNAs from optimized DNA sequences of both human and mouse constructs are more stable than the native and are suitable for initiation of translation. The constructs contain several sites for phosphorylation that could improve its degradation and subsequent entry into the MHC class I pathway. Addition of GPI anchor, myristoylation and ubiquitin signals or proline (P), glutamic acid (E), serine (S), threonine (T) (PEST)-like motifs at the N-terminal of constructs increase efficacy of the DNA vaccine. Close physical contact between the favorable immunogen and the suitable CpG oligodeoxynucleotides (CpG ODN) promotes immune response. Vectors for checking the expression of constructs in mammalian cells and for harboring the foreign genes as DNA vaccine are suggested.
authors.affiliation: Department of Biology, Shahed University, Tehran-Qom Express Way, Opposite Imam Khomeini's Shrine, Tehran-3319118651, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Jahangiri
authors.forename: Iraj
authors.initials: I
authors.lastname: Rasooli
authors.forename: Seyed Latif Mousavi
authors.initials: SL
authors.lastname: Gargari
authors.forename: Parviz
authors.initials: P
authors.lastname: Owlia
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Rahbar
authors.forename: Jafar
authors.initials: J
authors.lastname: Amani
authors.forename: Saeed
authors.initials: S
authors.lastname: Khalili
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology / Q000379:methods; D018899:CpG Islands / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000235:genetics / Q000276:immunology; D005805:Genes, MHC Class I / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006460:Hemolysin Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology; D055571:Secretory Pathway / Q000235:genetics / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D000485:Allergens; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D006680:HLA Antigens; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D016056:Immunodominant Epitopes; D012333:RNA, Messenger; D019444:Vaccines, DNA; C038705:hlyA protein, Listeria monocytogenes