title: Cross-reactivity of some commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns to DON- and ZEN-conjugated forms and metabolites.
abstract: Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) and metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) and nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra-performance liquid chromatography-tandem mass spectrometry method. The DON IACs showed cross-reactivity for nearly all DON derivatives tested. The ZEN IACs showed limited cross-reactivity to some of the ZEN derivatives. The IACs were evaluated for their potential use as sample clean-up for mycotoxins in serum.
authors.affiliation: Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium. aleksandrs.versilovskis@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Veršilovskis
authors.forename: B
authors.initials: B
authors.lastname: Huybrecht
authors.forename: E K
authors.initials: EK
authors.lastname: Tangni
authors.forename: L
authors.initials: L
authors.lastname: Pussemier
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
authors.forename: A
authors.initials: A
authors.lastname: Callebaut
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D007118:Immunoassay; D044967:Serum; D053719:Tandem Mass Spectrometry; D014255:Trichothecenes / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D015025:Zearalenone / Q000032:analysis / Q000737:chemistry / Q000276:immunology*
chemical_list: D014255:Trichothecenes; D015025:Zearalenone; C007262:deoxynivalenol