title: Determination of gadolinium-based magnetic resonance imaging contrast agents by micellar electrokinetic capillary chromatography.
abstract: MEKC with DAD was applied to detect six Gd-based contrasting agents (CAs) (Gd-DTPA-BMA (Omniscan), Gd-HPDO3A (ProHance), Gd-DOTA (Dotarem), Gd-AAZTA, Gd-BOPTA (Multihance) and Gd-DTPA (Magnevist)) commonly used in MRI diagnostics. The achieved LODs ranged between 0.40 and 20 μM and the optimized method gave excellent precision, especially when two internal standards were applied (less than 0.34 RSD% for migration time). The MEKC technique made it possible to determine the CAs in urine and serum samples of patients having a therapeutic dose. Due to the SDS content of the running buffer, the serum samples can be directly injected to analyze Gd-based CAs without interference of high protein content.
authors.affiliation: Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary.
authors.forename: Melinda
authors.initials: M
authors.lastname: Andrási
authors.forename: Attila
authors.initials: A
authors.lastname: Gáspár
authors.forename: Otilia
authors.initials: O
authors.lastname: Kovács
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Baranyai
authors.forename: Almos
authors.initials: A
authors.lastname: Klekner
authors.forename: Ernő
authors.initials: E
authors.lastname: Brücher
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003287:Contrast Media / Q000032:analysis / Q000737:chemistry*; D016903:Drug Monitoring; D005682:Gadolinium / Q000097:blood / Q000737:chemistry* / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009942:Organometallic Compounds / Q000097:blood / Q000737:chemistry* / Q000652:urine; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D003287:Contrast Media; D009942:Organometallic Compounds; D012967:Sodium Dodecyl Sulfate; D005682:Gadolinium