title: The potential role of metalloproteinases in neurogenesis in the gerbil hippocampus following global forebrain ischemia.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) have recently been considered to be involved in the neurogenic response of adult neural stem/progenitor cells. However, there is a lack of information showing direct association between the activation of MMPs and the development of neuronal progenitor cells involving proliferation and/or further differentiation in vulnerable (Cornus Ammoni-CA1) and resistant (dentate gyrus-DG) to ischemic injury areas of the brain hippocampus.
  
  
  RESULTS
  We showed that dynamics of MMPs activation in the dentate gyrus correlated closely with the rate of proliferation and differentiation of progenitor cells into mature neurons. In contrast, in the damaged CA1 pyramidal cells layer, despite the fact that some proliferating cells exhibited antigen specific characteristic of newborn neuronal cells, these did not attain maturity. This coincides with the low, near control-level, activity of MMPs. The above results are supported by our in vitro study showing that MMP inhibitors interfered with both the proliferation and differentiation of the human neural stem cell line derived from umbilical cord blood (HUCB-NSCs) toward the neuronal lineage.
  
  
  CONCLUSIONS
  Taken together, the spatial and temporal profiles of MMPs activity suggest that these proteinases could be an important component in neurogenesis-associated processes in post-ischemic brain hippocampus.
authors.affiliation: NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Luiza
authors.initials: L
authors.lastname: Wójcik-Stanaszek
authors.forename: Joanna
authors.initials: J
authors.lastname: Sypecka
authors.forename: Patrycja
authors.initials: P
authors.lastname: Szymczak
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Ziemka-Nalecz
authors.forename: Michel
authors.initials: M
authors.lastname: Khrestchatisky
authors.forename: Santiago
authors.initials: S
authors.lastname: Rivera
authors.forename: Teresa
authors.initials: T
authors.lastname: Zalewska
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology* / Q000473:pathology*; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000201:enzymology; D005849:Gerbillinae; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D008297:Male; D045726:Metalloproteases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D016548:Prosencephalon; D011480:Protease Inhibitors / Q000494:pharmacology; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology; D013234:Stem Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology
chemical_list: D011480:Protease Inhibitors; D045726:Metalloproteases