title: The influence of P. fluorescens cell morphology on the lytic performance and production of phage φIBB-PF7A.
abstract: This study aims at assessing the influence of Pseudomonas fluorescence cell morphology on the effectiveness and production of the lytic bacteriophage φIBB-PF7A. P. fluorescens were cultured as rods or as elongated cells by varying the temperature and rotary agitation conditions. Cells presented rod shape when grown at temperatures up to 25°C and also at 30°C under static conditions, and elongated morphology only at 30°C when cultures were grown under agitation. Elongated cells were 0.4 up to 27.9 μm longer than rod cells. Rod-shaped hosts were best infected by phages at 25°C which resulted in an 82% cell density reduction. Phage infection of elongated cells was successful, and the cell density reductions achieved was statistically similar (P > 0.05) to those obtained at the optimum growth temperature of P. fluorescens. Phage burst size varied with the cell growth conditions and was approximately 58 and 153 PFU per infected rod and elongated cells, grown at 160 rpm, at 25°C (the optimal temperature) and 30°C, respectively. Phage adsorption was faster to elongated cells, most likely due to the longer length of the host. The surface composition of rod and elongated cells is similar in terms of outer membrane proteins and lipopolysaccharide profiles. The results of this study suggest that the change of rod cells to an elongated morphology does not prevent cells from being attacked by phages and also does not impair the phage infection.
authors.affiliation: Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, Braga, Portugal. s.sillankorva@deb.uminho.pt
authors.forename: Sanna
authors.initials: S
authors.lastname: Sillankorva
authors.forename: Diana
authors.initials: D
authors.lastname: Pires
authors.forename: Hugo
authors.initials: H
authors.lastname: Oliveira
authors.forename: Peter
authors.initials: P
authors.lastname: Neubauer
authors.forename: Joana
authors.initials: J
authors.lastname: Azeredo
mesh_terms: D017105:Pseudomonas Phages / Q000254:growth & development / Q000502:physiology*; D011551:Pseudomonas fluorescens / Q000166:cytology* / Q000821:virology*; D013696:Temperature; D014779:Virus Replication