title: Detection of the dengue non-structural 1 antigen in cerebral spinal fluid samples using a commercially available enzyme-linked immunosorbent assay.
abstract: The involvement of the central nervous system in dengue infections has been reported in countries where the disease in endemic. The purpose of this study was to determine whether an enzyme-linked immunosorbent assay kit designed to detect the dengue NS1 antigen in serum was able to detect this antigen in cerebral spinal fluid (CSF) samples from patients with fatal outcomes. To evaluate the sensitivity of the kit, 26 dengue-positive CSF samples were used. The Pan-E Dengue Early kit was able to detect the NS1 antigen in 13 of 26 dengue-positive CSF samples, resulting in a sensitivity of 50% (95% confidence interval, 29.9-70.1%) and specificity of 100% (95% confidence interval, 75.3-100%). The kit was able to detect the NS1 antigen in CSF of individuals who had died of dengue. When used in combination with IgM, the detection rate rose to 92.3%. This study reports a method for rapidly detecting the dengue virus in CSF, thereby increasing the diagnosis of dengue fever cases with unusual neurological manifestations.
authors.affiliation: Central Public Health Laboratory of Ceará, Brazil. fernanda.montenegro@lacen.ce.gov.br
authors.forename: F M C
authors.initials: FM
authors.lastname: Araújo
authors.forename: R S N
authors.initials: RS
authors.lastname: Brilhante
authors.forename: L P G
authors.initials: LP
authors.lastname: Cavalcanti
authors.forename: M F G
authors.initials: MF
authors.lastname: Rocha
authors.forename: R A
authors.initials: RA
authors.lastname: Cordeiro
authors.forename: A C B
authors.initials: AC
authors.lastname: Perdigão
authors.forename: I S
authors.initials: IS
authors.lastname: Miralles
authors.forename: L C
authors.initials: LC
authors.lastname: Araújo
authors.forename: R M C
authors.initials: RM
authors.lastname: Araújo
authors.forename: E G
authors.initials: EG
authors.lastname: Lima
authors.forename: J J C
authors.initials: JJ
authors.lastname: Sidrim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000956:Antigens, Viral / Q000134:cerebrospinal fluid* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000134:cerebrospinal fluid; D007223:Infant; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017361:Viral Nonstructural Proteins / Q000134:cerebrospinal fluid* / Q000276:immunology; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic; D017361:Viral Nonstructural Proteins