title: AFM investigation of Pseudomonas aeruginosa lectin LecA (PA-IL) filaments induced by multivalent glycoclusters.
abstract: Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternate 90° orientation of LecA tetramers is proposed to describe the organisation of lectins and glycoclusters in the filaments.
authors.affiliation: Université de Lyon, Institut des Nanotechnologies de Lyon (INL), UMR CNRS 5270, site Ecole Centrale de Lyon, 36, avenue Guy de Collongue, 69134 Ecully cedex, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Sicard
authors.forename: Samy
authors.initials: S
authors.lastname: Cecioni
authors.forename: Maksym
authors.initials: M
authors.lastname: Iazykov
authors.forename: Yann
authors.initials: Y
authors.lastname: Chevolot
authors.forename: Susan E
authors.initials: SE
authors.lastname: Matthews
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Praly
authors.forename: Eliane
authors.initials: E
authors.lastname: Souteyrand
authors.forename: Anne
authors.initials: A
authors.lastname: Imberty
authors.forename: Sébastien
authors.initials: S
authors.lastname: Vidal
authors.forename: Magali
authors.initials: M
authors.lastname: Phaner-Goutorbe
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry*; D001665:Binding Sites; D047250:Calixarenes / Q000737:chemistry; D006031:Glycosylation; D018625:Microscopy, Atomic Force; D010636:Phenols / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; C102929:LecA protein, bacteria; D010636:Phenols; C121325:calix(4)arene; D047250:Calixarenes