title: Protective effects of fractional extracts from Panellus serotinus on non-alcoholic fatty liver disease in obese, diabetic db/db mice.
abstract: Non-alcoholic fatty liver disease (NAFLD) is emerging as the most common liver disease in industrialised countries. Various mushrooms have been used in Eastern folk medicine for the treatment of lifestyle diseases. We previously found that the dietary intake of powdered whole Panellus serotinus (Mukitake) alleviates NAFLD in obese, diabetic db/db mice. In the present study, we investigated the influence of Mukitake fractional extracts on the development of NAFLD in db/db mice. A significant reduction in the hepatic TAG content, macrovesicular hepatocytes and activities of key enzymes for de novo synthesis of the fatty acid was observed in both the water-soluble Mukitake extract (WE) diet and the ethanol-soluble Mukitake extract (EE) diet groups compared with the control diet group of the db/db mice. The serum level of monocyte chemoattractant protein-1 (MCP-1), which is known to exacerbate insulin resistance, was significantly decreased in the WE group. On the other hand, the serum level of adiponectin, which plays a protective role against the metabolic syndrome, was significantly increased in the EE group. Additionally, differential analysis between Mukitake and Shiitake, mycelia from the same family, using liquid chromatography time-of-flight MS technology revealed that only seven and five compounds exist in WE and EE from Mukitake, respectively. In conclusion, the present study demonstrated that Mukitake displays at least two different physiological actions that alleviate NAFLD: one through the reduction in inflammatory damage by its suppression in MCP-1 production and the other through an increase in level of serum adiponectin and the prevention of visceral fat accumulation.
authors.affiliation: Department of Applied Biochemistry and Food Science, Saga University, Saga 840-8502, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Inafuku
authors.forename: Koji
authors.initials: K
authors.lastname: Nagao
authors.forename: Saori
authors.initials: S
authors.lastname: Nomura
authors.forename: Bungo
authors.initials: B
authors.lastname: Shirouchi
authors.forename: Nao
authors.initials: N
authors.lastname: Inoue
authors.forename: Naoki
authors.initials: N
authors.lastname: Nagamori
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Toda
authors.forename: Teruyoshi
authors.initials: T
authors.lastname: Yanagita
mesh_terms: D052242:Adiponectin / Q000097:blood; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000627:therapeutic use; D019440:Anti-Obesity Agents / Q000737:chemistry / Q000627:therapeutic use*; D018932:Chemokine CCL2 / Q000097:blood / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D005234:Fatty Liver / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000627:therapeutic use*; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008518:Medicine, East Asian Traditional; D051379:Mice; D008817:Mice, Mutant Strains; D009765:Obesity / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D012995:Solubility; D014280:Triglycerides / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D000893:Anti-Inflammatory Agents; D019440:Anti-Obesity Agents; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D007004:Hypoglycemic Agents; D012333:RNA, Messenger; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha