title: Polydatin up-regulates Clara cell secretory protein to suppress phospholipase A2 of lung induced by LPS in vivo and in vitro.
abstract: BACKGROUND
  Lung injury induced by lipopolysaccharide (LPS) remains one of the leading causes of morbidity and mortality in children. The damage to membrane phospholipids leads to the collapse of the bronchial alveolar epithelial barrier during acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). Phospholipase A2 (PLA2), a key enzyme in the hydrolysis of membrane phospholipids, plays an important traumatic role in pulmonary inflammation, and Clara cell secretory protein (CCSP) is an endogenous inhibitor of PLA2. Our previous study showed that polydatin (PD), a monocrystalline extracted from a traditional Chinese medicinal herb (Polygonum cuspidatum Sieb, et Zucc), reduced PLA2 activity and sPLA2-IIA mRNA expression and mitigated LPS-induced lung injury. However, the potential mechanism for these effects has not been well defined. We have continued to investigate the effect of PD on LPS-induced expression of CCSP mRNA and protein in vivo and in vitro.
  
  
  RESULTS
  Our results suggested that the CCSP mRNA level was consistent with its protein expression. CCSP expression was decreased in lung after LPS challenge. In contrast, PD markedly increased CCSP expression in a concentration-dependent manner. In particular, CCSP expression in PD-pretreated rat lung was higher than in rats receiving only PD treatment.
  
  
  CONCLUSIONS
  These results indicated that up-regulation of CCSP expression causing inhibition of PLA2 activation may be one of the crucial protective mechanisms of PD in LPS-induced lung injury.
authors.affiliation: Department of Anesthesiology, Children's Hospital of Chongqing Medical University, Zhongshan Er Road NO136, Yuzhong District, Chongqing 40 0014, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Shiyu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhiyu
authors.forename: Ye
authors.initials: Y
authors.lastname: Mao
authors.forename: Bo
authors.initials: B
authors.lastname: Lin
authors.forename: Wang
authors.initials: W
authors.lastname: Lijia
authors.forename: Zhang
authors.initials: Z
authors.lastname: Tianbao
authors.forename: Chen
authors.initials: C
authors.lastname: Jie
authors.forename: Li
authors.initials: L
authors.lastname: Tingyu
mesh_terms: D000818:Animals; D002460:Cell Line; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008168:Lung / Q000201:enzymology* / Q000378:metabolism; D055370:Lung Injury / Q000378:metabolism; D008297:Male; D064801:Phospholipase A2 Inhibitors; D054467:Phospholipases A2 / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013267:Stilbenes / Q000494:pharmacology*; D015854:Up-Regulation; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
chemical_list: D005960:Glucosides; D008070:Lipopolysaccharides; D064801:Phospholipase A2 Inhibitors; D012333:RNA, Messenger; D013267:Stilbenes; D014598:Uteroglobin; D054467:Phospholipases A2; C058229:polydatin