title: Orthogonal polarization spectral imaging: a novel tool for examination of microcirculatory changes in the testis.
abstract: Testicular torsion is a surgical emergency in urologic practice. Many  models have demonstrated the relationship between the degree and duration of  the torsion and the subsequent damages of the testis, but there are as yet no  suitable methods for visualization of the testicular microcirculation in this  condition. We aimed to use orthogonal polarization spectral (OPS) imaging to  observe the microcirculatory changes in the testis after ischemia-reperfusion  (I/R) challenge, and to compare this technique with that of fluorescence  intravital video microscopy (IVM). Twelve rats subjected to 60-minute ischemia  following 720° testicular torsion were divided into two groups for IVM or  OPS imaging examinations of the microcirculatory network of the testis at 60,  120, 180, and 240 minutes after reestablishment of perfusion. Significant  microcirculatory failure was demonstrated after I/R in both groups. The  absolute values of the microcirculatory parameters recorded with the OPS and  IVM imaging methods did not differ statistically. The microcirculatory  disturbances are present during the later phases of testicular torsion. OPS  imaging technique represents an accurate noninvasive method to detect  physiologic and pathophysiologic changes in the microcirculation of the  testis.
authors.affiliation: Department of Urology, University of Szeged, Szeged, Hungary. bajory@freemail.hu
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Bajory
authors.forename: Andrea
authors.initials: A
authors.lastname: Szabó
authors.forename: Gábor
authors.initials: G
authors.lastname: Deák
authors.forename: Renáta
authors.initials: R
authors.lastname: Varga
authors.forename: László
authors.initials: L
authors.lastname: Pajor
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods*; D008297:Male; D008833:Microcirculation; D008859:Microscopy, Polarization / Q000379:methods*; D018715:Microscopy, Video / Q000379:methods*; D051381:Rats; D015427:Reperfusion Injury / Q000175:diagnosis; D013086:Spermatic Cord Torsion / Q000175:diagnosis; D013737:Testis / Q000098:blood supply*