title: Transcriptome-wide identification and stress properties of the 14-3-3 gene family in cotton (Gossypium hirsutum L.).
abstract: 14-3-3s are a class of conserved proteins in eukaryotes involved in almost all cellular processes. Published studies have shown that this family plays a role in response to stress conditions. In this study, comparative genomics identified thirty-one 14-3-3 cDNAs encoding 25 unique proteins with transcripts that were sufficiently divergent in the coding regions to define different 14-3-3 members. Phylogenetic analysis indicated that the cotton 14-3-3 family was similar to the family in the plant model species Arabidopsis and rice. The multiple copies of 14-3-3 genes in cotton are suggested to be the result of a recent duplication event after the divergence of cotton from its progenitor species. 14-3-3s are differentially expressed in a range of cotton organs as well as under different environmental conditions. Salinity and drought stress significantly induced the altered expression of 14-3-3 genes, suggesting that 14-3-3s play a role in cotton response to environmental abiotic stress. The 14-3-3 gene CGF14-4 was more sensitive to both drought and salinity stress with a 10.2-fold change under 1% of PEG treatment, while other 14-3-3s responded only to either drought or salinity stress. These results implied that specific isoforms of 14-3-3s play different regulatory roles in stress response besides their role in development. 14-3-3s were more sensitive to abiotic stress in roots than in leaves, suggesting that the root is more sensitive to stress treatment than leaves. The differential expression pattern of 14-3-3s also suggests the occurrence of functional divergence among specific isoforms.
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC, 27858, USA.
authors.forename: Guiling
authors.initials: G
authors.lastname: Sun
authors.forename: Fuliang
authors.initials: F
authors.lastname: Xie
authors.forename: Baohong
authors.initials: B
authors.lastname: Zhang
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics*; D001483:Base Sequence; D003681:Dehydration / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D055049:Salt Tolerance / Q000235:genetics; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D048948:14-3-3 Proteins; D010940:Plant Proteins