title: Interaction of polyamidoamine (PAMAM) succinamic acid dendrimers generation 4 with human serum albumin.
abstract: Dendrimers, a relatively new group of highly branched three dimensional polymers, are intensively investigated to use them in biomedical and physicochemical sciences. Their specific architecture gives them the ability to interact with many different types of molecules. In our studies the interaction between PAMAM succinamic acid dendrimers generation 4 (PAMAM-SAH G4) and human serum albumin (HSA) was examined. Experiments showed that a single molecule of a HSA can bind approximately 6 particles of dendrimers. The fluorescence studies demonstrated that dendrimers lead to a decrease in protein fluorescence but changes in fluorescence anisotropy were not observed. Alterations in the spectrum of circular dichroism indicated changes in the secondary protein structure. The results clearly show that this generation of dendrimers possesses a strong ability to interact with human serum albumin.
authors.affiliation: Department of General Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. sekowski100@vp.pl
authors.forename: Szymon
authors.initials: S
authors.lastname: Sekowski
authors.forename: Adam
authors.initials: A
authors.lastname: Buczkowski
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Palecz
authors.forename: Teresa
authors.initials: T
authors.lastname: Gabryelak
mesh_terms: D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism*; D004347:Drug Interactions; D006801:Humans; D008954:Models, Biological; D009757:Nylons / Q000737:chemistry* / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D019802:Succinic Acid / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D009757:Nylons; C520550:PAMAM-G4; C531249:Poly(amidoamine); D011073:Polyamines; D012709:Serum Albumin; D019802:Succinic Acid