title: Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules.
abstract: We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.
authors.affiliation: Nanoscience Center, Department of Physics, University of Jyväskylä, P.O. Box 35, FI-40014, Jyväskylä, Finland. veikko.linko@jyu.fi
authors.forename: Veikko
authors.initials: V
authors.lastname: Linko
authors.forename: Jenni
authors.initials: J
authors.lastname: Leppiniemi
authors.forename: Boxuan
authors.initials: B
authors.lastname: Shen
authors.forename: Einari
authors.initials: E
authors.lastname: Niskanen
authors.forename: Vesa P
authors.initials: VP
authors.lastname: Hytönen
authors.forename: J Jussi
authors.initials: JJ
authors.lastname: Toppari
mesh_terms: D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004566:Electrodes; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D006046:Gold; D004247:DNA; D004259:DNA-Directed DNA Polymerase