title: Comparison of HPLC and UV spectrophotometric methods for the determination of cefuroxime sodium in pharmaceutical products.
abstract: This paper describes the development and evaluation of a HPLC and UV spectrophotometric methods to quantify cefuroxime sodium in injectables. HPLC analysis were carried out using a C18 Wat 054275 column and a mobile phase composed of methanol and water (70:30), with a flow rate of 0.8 mL/min and UV detection at 280 nm. For the spectrophotometric analysis, water was used as solvent and the wavelength of 280 nm was selected for the detection. Both methods were found to quantify cefuroxime sodium in injectables accurately. Therefore HPLC and UV methods presented the most reliable results for the analyses of injectables.
authors.affiliation: Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Araraquara UNESP, Rodovia AraraquaraJaú, km 1, CEP 14801-902, Araraquara SP, Brazil.
authors.forename: D C M
authors.initials: DC
authors.lastname: Vieira
authors.forename: H R N
authors.initials: HR
authors.lastname: Salgado
mesh_terms: D002444:Cefuroxime / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D002444:Cefuroxime