title: Efficacy of large doses of IL-2-activated human leukocyte antigen haploidentical peripheral blood stem cells on refractory metastatic renal cell carcinoma.
abstract: Traditional immunotherapy for patients with refractory metastatic renal cell carcinoma (RCC) is limited because the tumors themselves induce immunosuppression. The aim of this article was to evaluate the clinical efficacy of the infusion of a high dose of interleukin (IL)-2-activated allogeneic haploidentical peripheral blood stem cells (haplo-PBSCs) in patients with advanced intractable RCC. Ten advanced RCC patients and their haploidentical relatives, who were haplo-PBSC donors, were enrolled in this study. All patients accepted one cycle of activated haplo-PBSCs. The clinical and immunologic responses were evaluated. A range from 2.3 to 5.5×10(10) of activated haplo-PBSCs were harvested after exposure to recombinant human IL-2 (rhIL-2), along with a significant increase in the proportion of natural killer cells and activated lymphocytes (CD69+ and CD25+). Enhanced cytotoxicity of haplo-PBSCs for RCC was also observed. After treatment, 2 (2/10) cases of partial remission, 6 (6/10) cases of stable disease, and 2 (2/10) cases of progressive disease were identified in these 10 patients. The median progression-free survival of the 10 patients was 5.5 months (3-14 months). The adoptive transfusion of IL-2-activated haplo-PBSCs can induce sustained antitumor effects for advanced intractable RCC patients who have had no response to conventional immunotherapy.
authors.affiliation: Biotherapy Center of Tianjin Cancer Institute and Hospital, Tianjin Medical University, Tiyuanbei, Huanhuri Road, Hari District, Tianjin, China.
authors.forename: Shui
authors.initials: S
authors.lastname: Cao
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Wang
authors.forename: Xiu-Bao
authors.initials: XB
authors.lastname: Ren
authors.forename: Jin-Pu
authors.initials: JP
authors.lastname: Yu
authors.forename: Bao-Zhu
authors.initials: BZ
authors.lastname: Ren
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002292:Carcinoma, Renal Cell / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006239:Haplotypes; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000276:immunology*; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000276:immunology / Q000627:therapeutic use*; D007680:Kidney Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D016896:Treatment Outcome
chemical_list: D006680:HLA Antigens; D007376:Interleukin-2