title: [A novel method for fast determination of components in Guizhi Fuling capsule by near infrared spectroscopy].
abstract: OBJECTIVE
  To develop a new method to rapidly determine and identify Guizhi Fuling capsule by portable acousto-optic tunable filter-near infrared spectroscopy.
  
  
  METHODS
  The qualitative model was set up using principal component analysis. The correlation models between the NIR spectra and the reference values of five major constituents were obtained with partial least squares method.
  
  
  RESULTS
  The identifying model accurately identified Guizhi Fuling capsule, and quantitative analytical models could precisely predicted the content of ellagic acid, baicalin, benzoylpaenoniflorin, cinnamaldehyde, and paeonol. The correlation coefficients of the calibration models were 0.924 2, 0.938 4, 0.924 2, 0.933 6, 0.934 7, the validation set coefficients of the calibration were 0.924 2, 0.938 4, 0.924 2, 0.933 6, 0.934 7, and the RMSEP were 1.138%, 3.014%, 0.751%, 0.625%, 3.455%, 1.363%, respectively. The results of external validation showed no significant difference between the predictive and the determining values by t-test.
  
  
  CONCLUSIONS
  The method is accurate, rapid and non-destructive, and can be used for determining and identifying Guizhi Fuling capsule.
authors.affiliation: Jiangsu Kanion Pharmaceutical Co. Ltd. , Lianyungang 222001, China. gkm107@163.com
authors.forename: Kaimin
authors.initials: K
authors.lastname: Gong
authors.forename: Jiachun
authors.initials: J
authors.lastname: Li
authors.forename: Lianmin
authors.initials: L
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Yu'an
authors.initials: Y
authors.lastname: Bi
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Wang
authors.forename: Chenfeng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000098:Acetophenones / Q000032:analysis*; D000171:Acrolein / Q000031:analogs & derivatives* / Q000032:analysis; D002138:Calibration; D002214:Capsules / Q000737:chemistry; D004341:Drug Evaluation / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D004610:Ellagic Acid / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D016018:Least-Squares Analysis; D008956:Models, Chemical; D025341:Principal Component Analysis / Q000379:methods*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D000098:Acetophenones; D002214:Capsules; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C525079:guizhi-fuling; D004610:Ellagic Acid; C038044:baicalin; C013638:paeonol; D000171:Acrolein; C012843:cinnamaldehyde