title: Babesia canis rossi infection in a Texas dog.
abstract: A 5-month-old intact male Boerboel dog, imported from South Africa 1 week previously, was presented to a Texas veterinarian for lethargy, anorexia, and labored breathing. The dog was febrile, anemic, leukopenic, thrombocytopenic, and slightly azotemic. Results of the IDEXX SNAP-4Dx enzyme immunoassay were negative for Dirofilaria immitis antigen and antibodies against Ehrlichia canis, Borrelia burgdorferi, and Anaplasma phagocytophilum. An EDTA blood sample analyzed at Oklahoma State University Center for Veterinary Health Sciences revealed nonregenerative anemia, neutropenia, and large protozoal piroplasms in 0.7% of the RBCs. Piroplasms were 2-5μm long and varied in shape from round to oval to piriform; extracellular merozoites were also observed. Nested PCR was performed on DNA extracted from blood using primers that amplify the 18s rRNA gene from all known Babesia species, and the product was sequenced. Basic Local Alignment Search Tool analysis of the 437 base sequence revealed 99-100% similarity to Babesia canis rossi, 92-93% similarity to Babesia canis canis, and 92% similarity to Babesia canis vogeli. The dog responded well to treatment with imidocarb. PCR analysis of a second blood sample 2 weeks later was negative for Babesia spp. DNA. This case represents the first diagnosis of B. canis rossi infection in the United States.
authors.affiliation: Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA. robin.allison@okstate.edu
authors.forename: Robin W
authors.initials: RW
authors.lastname: Allison
authors.forename: Todd J
authors.initials: TJ
authors.lastname: Yeagley
authors.forename: Kristina
authors.initials: K
authors.lastname: Levis
authors.forename: Mason V
authors.initials: MV
authors.lastname: Reichard
mesh_terms: D000740:Anemia / Q000097:blood / Q000662:veterinary*; D000818:Animals; D000981:Antiprotozoal Agents / Q000627:therapeutic use; D001403:Babesia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D001483:Base Sequence; D016054:DNA, Protozoan / Q000097:blood / Q000235:genetics; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis / Q000469:parasitology; D004285:Dogs; D007095:Imidocarb / Q000627:therapeutic use; D008297:Male; D008969:Molecular Sequence Data; D009503:Neutropenia / Q000097:blood / Q000662:veterinary*; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013019:South Africa; D013781:Texas; D014195:Travel
chemical_list: D000981:Antiprotozoal Agents; D016054:DNA, Protozoan; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S; D007095:Imidocarb