title: [Recombination between human leukocyte antigen -A and -C loci within two Chinese Han families].
abstract: OBJECTIVE
  To investigate the recombination events between human leukocyte antigen (HLA) loci within two families.
  
  
  METHODS
  Identification of HLA-A, -C, -B, -DRB1 and -DQB1 loci was firstly carried out using polymerase chain reaction-sequence specific oligonucleotide. Then HLA high resolution typing was performed using polymerase chain reaction sequencing-based typing. The recombination between HLA loci was identified by family genetic analysis. The parentage possibility was analyzed by short tandom repeat technique.
  
  
  RESULTS
  Recombination between the HLA-A and C loci was identified within two families. One individual inherited a paternal haplotype that was the result of a recombination event between the father's HLA-A and -C loci on his chromosomes. The other individual inherited a maternal haplotype that was the result of a recombination event between the mother's HLA-A and -C loci. The high parentage possibilities were obtained in the family members.
  
  
  CONCLUSIONS
  The recombination events of HLA-A and -C have been found in two Chinese families, which may help further study on the mechanism of HLA recombination.
authors.affiliation: Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of Ministry of Health, Hangzhou, Zhejiang 310006, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhe-dong
authors.initials: ZD
authors.lastname: Han
authors.forename: Nan-ying
authors.initials: NY
authors.lastname: Chen
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Fa-ming
authors.initials: FM
authors.lastname: Zhu
authors.forename: Hang-jun
authors.initials: HJ
authors.lastname: Lv
authors.forename: Li-xing
authors.initials: LX
authors.lastname: Yan
mesh_terms: D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D002681:China / Q000208:ethnology; D005006:Ethnicity / Q000235:genetics*; D005260:Female; D056426:Genetic Loci / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008297:Male; D010375:Pedigree; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D015234:HLA-A Antigens; D015236:HLA-C Antigens