title: Sequencing circulating miRNA in maternal plasma with modified library preparation.
abstract: OBJECTIVE
  Circulating microRNAs in maternal plasma as one type of the cell free nucleotide acid revealed its potential for non-invasive prenatal diagnosis. The next generation sequencing technology provides promising approach detecting miRNA for such purpose.
  
  
  METHODS
  In this present study, a modified library preparation method for SOLiD sequencing technology was developed and maternal plasma miRNA from single and twin pregnancies was analyzed. Quantitative PCR was carried out for comparison.
  
  
  RESULTS
  Results showed that the sequenced data was improved remarkably with this modified library preparation method; different types and levels of miRNA expression were found in twin pregnancy compared with control. Several miRNAs were validated that remarkably changed in twin pregnancy.
  
  
  CONCLUSIONS
  It is indicated that miRNAs might involve the process of pregnancy such as the generation of twin pregnancy, and it also suggested that the specific miRNAs could act as potential biomarkers for clinical diagnosis and therapy.
authors.affiliation: Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing, 210096, China. geqinyu@seu.edu.cn
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
authors.forename: Hailing
authors.initials: H
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Tu
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA; D055815:Young Adult
chemical_list: D035683:MicroRNAs