title: The effect of sedation on D(+)-xylose absorption tests in 6 normal horses.
abstract: BACKGROUND
  D(+)-xylose absorption tests are commonly performed when investigating suspected small intestinal malabsorption in the horse. The test involves the administration of a D(+)-xylose solution via a nasogastric tube followed by serial blood sampling to determine its rate of absorption. In some horses, nasogastric intubation cannot be safely performed without prior administration of a sedative. Due to its short duration of action, the α(2) agonist xylazine is commonly used for this purpose. However, α(2) agonists have also been reported to influence the rate of gastric emptying as well as small intestinal motility patterns.
  
  
  OBJECTIVE
  To evaluate if prior sedation with xylazine would influence the rate of absorption of D(+)-xylose in 6 normal Standardbred horses in a randomised cross-over study.
  
  
  METHODS
  D(+)-xylose was administered by nasogastric intubation at a dose rate of 0.5 g/kg bwt given as a 10% solution with water while xylazine was administered iv at a dose rate of 0.5 mg/kg bwt. A heparinised blood sample was collected prior to administration of D(+)-xylose (and xylazine when used) and then at 30, 45, 60, 75, 90, 120, 150, 180 and 240 min following administration. Samples were immediately analysed using a modified colorimetric micro method. The cumulative amount of D(+)-xylose absorbed at each time point with and without prior sedation were. The significance rate was set at P<0.05.
  
  
  RESULTS
  The study failed to demonstrate a statistically significant difference in the amount of D(+)-xylose absorbed between sedated and unsedated animals, although there was a tendency for a less rapid initial uptake with prior sedation.
  
  
  CONCLUSIONS
  This study suggests that prior sedation with xylazine will not significantly affect the result of a D(+)-xylose absorption test in the normal horse.
authors.affiliation: Norwegian School of Veterinary Science, Department of Companion Animal Clinical Sciences, Norway. Constanze.Fintl@nvh.no
authors.forename: C
authors.initials: C
authors.lastname: Fintl
authors.forename: C F
authors.initials: CF
authors.lastname: Ihler
mesh_terms: D000818:Animals; D016292:Procedural Sedation / Q000662:veterinary*; D018592:Cross-Over Studies; D004347:Drug Interactions; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology; D006736:Horses / Q000378:metabolism*; D006993:Hypnotics and Sedatives / Q000008:administration & dosage / Q000494:pharmacology*; D014991:Xylazine / Q000008:administration & dosage / Q000494:pharmacology*; D014994:Xylose / Q000493:pharmacokinetics*
chemical_list: D006993:Hypnotics and Sedatives; D014991:Xylazine; D014994:Xylose