title: Diesel exhaust particles suppress expression of sex steroid hormone receptors in TM3 mouse Leydig cells.
abstract: Murine Leydig TM3 cells, derived from mouse testis, were exposed to diesel exhaust particles (DEP) and DEP components for 24h. mRNA expression of estrogen receptor (ER) α and CYP1A1 were then measured by real-time PCR. Exposure to DEP (10μg/ml) reduced ERα mRNA expression by approximately 50% and increased P450 1A1 mRNA expression by approximately fivefold. Cycloheximide (CHX), an inhibitor of protein synthesis, abolished the DEP-induced effects on ERα mRNA expression. Thus, protein synthesis mediated the DEP-induced reduction of ERα mRNA expression.
authors.affiliation: Department of Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-0022, Japan; Department of Health and Sciences, Oita University of Nursing and Health Sciences, 2944-9 Megusuno, Oita, Oita 870-1201, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Sayoko
authors.initials: S
authors.lastname: Hirano
authors.forename: Kanako
authors.initials: K
authors.lastname: Shikagawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirata
authors.forename: Saori
authors.initials: S
authors.lastname: Rokuta
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Takano
authors.forename: Takamichi
authors.initials: T
authors.lastname: Ichinose
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda