title: Inhibitory effect of metformin on oxidation of NADH-dependent substrates in rat liver homogenate.
abstract: Metformin is widely used in the treatment of Type 2 diabetes, however, mechanisms of its antihyperglycemic effect were not yet fully elucidated. Complex I of mitochondrial respiration chain is considered as one of the possible targets of metformin action. In this paper, we present data indicating that the inhibitory effect of metformin can be tested also in liver homogenate. Contrary to previous findings on hepatocytes or mitochondria under our experimental conditions, lower metformin concentrations and shorter time of preincubation give significant inhibitory effects. These conditions enable to study the mechanism of the inhibitory effect of metformin in small samples of biological material (50-100 mg wet weight) and compare more experimental groups of animals because isolation of mitochonria is unnecessary.
authors.affiliation: Department of Metabolism and Diabetes, Centre of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic. Eliska.Palenickova@ikem.cz
authors.forename: E
authors.initials: E
authors.lastname: Páleníčková
authors.forename: M
authors.initials: M
authors.lastname: Cahová
authors.forename: Z
authors.initials: Z
authors.lastname: Drahota
authors.forename: L
authors.initials: L
authors.lastname: Kazdová
authors.forename: M
authors.initials: M
authors.lastname: Kalous
mesh_terms: D000818:Animals; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D008687:Metformin / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009249:NADP; D008687:Metformin; D010100:Oxygen