title: Fabrication of a model continuously graded co-electrospun mesh for regeneration of the ligament-bone interface.
abstract: Current scaffolds for the regeneration of anterior cruciate ligament injuries are unable to capture intricate mechanical and chemical gradients present in the natural ligament-bone interface. As a result, stress concentrations can develop at the scaffold-bone interface, leading to poor osseointegration. Hence, scaffolds that possess appropriate mechano-chemical gradients would help establish normal loading properties at the interface, while promoting scaffold integration with bone. With the long-term goal of investigating regeneration of the ligament-bone interface, this feasibility study aimed to fabricate a continuously graded mesh. Specifically, graded meshes were fabricated by co-electrospinning nanohydroxyapatite/polycaprolactone (nHAP-PCL) and poly(ester urethane) urea elastomer solutions from offset spinnerets. Next, mineral crystallites were selectively deposited on the nHAP-PCL fibers by treatment with a 5× simulated body fluid (5× SBF). X-ray diffraction and energy-dispersive spectroscopy indicated calcium-deficient hydroxyapatite-like mineral crystallites with an average Ca/P ratio of 1.48. Tensile testing demonstrated the presence of a mechanical gradient, which became more pronounced upon treatment with 5× SBF. Finally, biocompatibility of the graded meshes was verified using an MC3T3-E1 osteoprogenitor cell line. The study demonstrates that graded meshes, for potential application in interfacial tissue engineering, can be fabricated by co-electrospinning.
authors.affiliation: Department of Chemical Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Satyavrata
authors.initials: S
authors.lastname: Samavedi
authors.forename: C
authors.initials: C
authors.lastname: Olsen Horton
authors.forename: Scott A
authors.initials: SA
authors.lastname: Guelcher
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Goldstein
authors.forename: Abby R
authors.initials: AR
authors.lastname: Whittington
mesh_terms: D001842:Bone and Bones; D008022:Ligaments; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D012038:Regeneration; D014961:X-Ray Diffraction