title: Adjuvant properties of Cytosine-phosphate-guanosine oligodeoxynucleotide in combination with various polycations in an ovalbumin-vaccine model.
abstract: Oligonucleotides containing CpG motifs (cytosine-phosphate-guanosine oligodeoxynucleotide [CpG ODN]) display strong immunostimulatory effects, and polycations have been previously reported as cellular delivery system. In the present study, we investigated the adjuvant properties of combinations of a CpG ODN with various polycations (poly-arginine, poly-lysine, poly-histidine, or chitosan) in an ovalbumin vaccination model. We showed that, when combined to CpG ODN, poly-arginine and poly-histidine, but not poly-lysine or chitosan, enhanced efficiently both the IgG antibody production and the number of splenocytes secreting interferon-gamma after stimulation with a CD8+ T cell-restricted peptide. Interestingly, CpG ODN-poly-arginine, which was the most efficient, compared favorably to the complete Freund's adjuvant and aluminium salts and induced no local toxicity, making this combination a very attractive adjuvant for vaccines.
authors.affiliation: Laboratoire de Recherches Biochirurgicales, Hôpital Européen Georges Pompidou, Université Paris Descartes , Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Maubant
authors.forename: Claire
authors.initials: C
authors.lastname: Banissi
authors.forename: Samantha
authors.initials: S
authors.lastname: Beck
authors.forename: Anne
authors.initials: A
authors.lastname: Chauvat
authors.forename: Antoine F
authors.initials: AF
authors.lastname: Carpentier
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000534:Alum Compounds / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology; D004355:Drug Stability; D024202:Electrophoretic Mobility Shift Assay; D005620:Freund's Adjuvant / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D007106:Immune Sera / Q000378:metabolism*; D007111:Immunity, Cellular / Q000187:drug effects; D056724:Immunity, Humoral / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D010047:Ovalbumin / Q000276:immunology*; D010316:Particle Size; D010455:Peptides / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology*; D000071228:Polyelectrolytes; D011107:Polylysine / Q000494:pharmacology; D014611:Vaccination / Q000379:methods*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D048271:Chitosan; D005620:Freund's Adjuvant; D006639:Histidine; D007106:Immune Sera; D009838:Oligodeoxyribonucleotides; D010047:Ovalbumin; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D011107:Polylysine; C408982:CPG-oligonucleotide; C015462:polyarginine; C033223:polyhistidine; C041524:aluminum sulfate