title: Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe.
abstract: It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.
authors.affiliation: Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environment Science, Hebei University, Baoding 071002, Hebei Province, PR China.
authors.forename: Jiangyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yucong
authors.initials: Y
authors.lastname: Wang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Jia
authors.forename: Jingli
authors.initials: J
authors.lastname: Yan
mesh_terms: D015342:DNA Probes / Q000737:chemistry*; D006801:Humans; D008168:Lung / Q000378:metabolism; D035683:MicroRNAs / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012265:Ribonucleotides / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D012265:Ribonucleotides