title: The role of cell surface markers and enamel matrix derivatives on human periodontal ligament mesenchymal progenitor responses in vitro.
abstract: Periodontitis is a chronic-, infectious-disease of the human periodontium that is characterized by the loss of supporting tissues surrounding the tooth such as the periodontal ligament (PDL), cementum and alveolar bone. Regeneration of the periodontium is dependent on the participation of mesenchymal stem/stromal cells (MSC) resident in the PDL. Enamel matrix derivative (EMD), an extract from immature porcine enamel rich in amelogenin protein but that also contain bone morphogenetic protein (BMP), is used to treat periodontal defects. The effects of EMD on MSC cells of the PDL are not well characterized. In this in vitro study, we identify PDL progenitor cells from multiple individuals and demonstrate that EMD stimulates them. We show that the effect of EMD on cell proliferation and migration is mediated through the amelogenin it contains, while the differentiation of these progenitor cells to cell types of mineralized tissue is mainly due to BMP signaling.
authors.affiliation: Faculty of Odontology, University Paul-Sabatier, 3 Chemin des Maraîchers, 31062 Toulouse Cedex, France. philippe.kemoun@wanadoo.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Kémoun
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.forename: Malcolm L
authors.initials: ML
authors.lastname: Snead
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Rue
authors.forename: Bruno
authors.initials: B
authors.lastname: Courtois
authors.forename: Frédéric
authors.initials: F
authors.lastname: Vaysse
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Salles
authors.forename: Gérard
authors.initials: G
authors.lastname: Brunel
mesh_terms: D053523:Amelogenin / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003743:Dental Enamel / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D013552:Swine; D014945:Wound Healing
chemical_list: D053523:Amelogenin; D019485:Bone Morphogenetic Proteins