title: Sensory stimuli induce nuclear translocation and phosphorylation of nuclear factor κ B in primary sensory neurons of mice.
abstract: Nuclear factor kappa B (NF-κB) is a transcription factor, which is translocated to the nucleus when activated. Herein, we demonstrate immunohistochemically that electrical, chemical, and thermal stimuli, applied to the skin of mice, all induced nuclear translocation and phosphorylation of NF-κB in dorsal root ganglia (DRG) neurons. The latency of this response was short, with effects observable in as little as 3min following stimulation. Few nuclear phospho-NF-κB-positive neurons were observed in DRG innervating unstimulated regions. These results suggest somatosensory stimuli quickly induce NF-κB-mediated gene transcription in DRG, and phospho-NF-κB could be a suitable histological marker for activated DRG neurons.
authors.affiliation: Department of Anesthesiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Fujikawa
authors.forename: Norihiro
authors.initials: N
authors.lastname: Nishitani
authors.forename: Takae
authors.initials: T
authors.lastname: Ibuki
authors.forename: Shigeo
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsumura
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000502:physiology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D004558:Electric Stimulation / Q000379:methods; D005727:Ganglia, Spinal / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D011984:Sensory Receptor Cells / Q000378:metabolism*; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D016328:NF-kappa B