title: Cytogenetic analysis of CpG-oligonucleotide DSP30 plus Interleukin-2-Stimulated canine B-Cell lymphoma cells reveals the loss of one X Chromosome as the sole abnormality.
abstract: Human and canine lymphoid neoplasms are characterized by non-random cytogenetic abnormalities. However, due to the low mitotic activity of the B cells, cytogenetic analyses of B-cell lymphoid proliferations are difficult to perform. In the present study we stimulated canine B-cell lymphoma cells with the immunostimulatory CpG-oligonucleotide DSP30 in combination with interleukin-2 (IL-2) and obtained an adequate number of metaphases. Cytogenetic analyses revealed the loss of one X chromosome as the sole cytogenetic aberration. Chromosome analysis of the corresponding blood showed a normal female karyotype. Monosomy X as the sole clonal chromosomal abnormality is found in human hematopoietic malignancies as well, thus the dog may serve as a promising animal model.
authors.affiliation: Small Animal Clinic and Research Cluster REBIRTH, University of Veterinary Medicine Hannover, Germany. nicola.reimann-berg @ tiho-hannover.de
authors.forename: N
authors.initials: N
authors.lastname: Reimann-Berg
authors.forename: H
authors.initials: H
authors.lastname: Murua Escobar
authors.forename: Y
authors.initials: Y
authors.lastname: Kiefer
authors.forename: R
authors.initials: R
authors.lastname: Mischke
authors.forename: S
authors.initials: S
authors.lastname: Willenbrock
authors.forename: N
authors.initials: N
authors.lastname: Eberle
authors.forename: I
authors.initials: I
authors.lastname: Nolte
authors.forename: J
authors.initials: J
authors.lastname: Bullerdiek
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003582:Cytogenetics / Q000379:methods*; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology; D004285:Dogs; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D007621:Karyotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology*; D008213:Lymphocyte Activation; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology; D008677:Metaphase; D009006:Monosomy; D009841:Oligonucleotides / Q000494:pharmacology; D014960:X Chromosome / Q000737:chemistry* / Q000235:genetics
chemical_list: C539572:CpG-ODN DSP30; D007376:Interleukin-2; D009841:Oligonucleotides