title: Functionalized polystyrene supports for solid-phase synthesis of glycyl-, alanyl-, and isoleucyl-RNA conjugates as hydrolysis-resistant mimics of peptidyl-tRNAs.
abstract: RNA-peptide conjugates that mimic amino acid-charged tRNAs and peptidyl-tRNAs are of high importance for structural and functional investigations of ribosomal complexes. Here, we present the synthesis of glycyl-, alanyl-, and isoleucyladenosine modified solid supports that are eligible for the synthesis of stable 3'-aminoacyl- and 3'-peptidyl-tRNA termini with an amide instead of the natural ester linkage. The present work significantly expands the range of accessible peptidyl-tRNA mimics for ribosomal studies.
authors.affiliation: Institute of Organic Chemistry and Center for Molecular Biosciences CMBI, University of Innsbruck, Innsbruck, Austria.
authors.forename: Jessica
authors.initials: J
authors.lastname: Steger
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
mesh_terms: D000241:Adenosine / Q000737:chemistry; D000409:Alanine / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D006868:Hydrolysis; D007532:Isoleucine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D012346:RNA, Transfer, Amino Acyl / Q000138:chemical synthesis* / Q000737:chemistry; D012270:Ribosomes / Q000378:metabolism; D060327:Solid-Phase Synthesis Techniques
chemical_list: D010455:Peptides; D011137:Polystyrenes; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; D007532:Isoleucine; D012313:RNA; D000241:Adenosine; D000409:Alanine; D005998:Glycine