title: Excretion kinetics of urinary 3-hydroxybenzo[a]pyrene following dietary exposure to benzo[a]pyrene in humans.
abstract: Urinary 3-hydroxy-benzo[a]pyrene (3-OHBaP) is considered as an exposure marker for assessing carcinogenic risks as it is a metabolite of benzo[a]pyrene (BaP). A controlled human study was conducted to examine the kinetics of urinary 3-OHBaP after consuming charcoal-barbecued meat. Two feeding experiments were performed, with meat doses of 15 and 30 g/kg (experiments 1 and 2, respectively). All voided urine over 7 days was collected and analyzed. The background urinary 3-OHBaP concentration was 0.002-0.085 ng/g creatinine, with large inter-individual (80-100%) and intra-individual of (90-100%) variations. The background amount of 3-OHBaP excreted by the current subjects was 40-50 pg/day. The amounts of urinary 3-OHBaP excreted within 12 h post-exposure increased significantly (P < 0.05) from background only in experiment 1. The proportion of the administered BaP dose that was excreted as urinary 3-OHBaP within 12 h post-exposure was 0.006 and 0.0012% in experiments 1 and 2, respectively. The excretion ratio declined as the dose increased. Urinary 3-OHBaP can be used to assess dietary exposure to BaP, but it may be not suitable for low-dose scenarios because of the low urinary excretion proportion and high variability of the background.
authors.affiliation: Department of Safety, Health and Environmental Engineering, Hungkuang University, Taichung, Taiwan. yc@yuntech.edu.tw
authors.forename: Yeh-Chung
authors.initials: YC
authors.lastname: Chien
authors.forename: Chun-Ting
authors.initials: CT
authors.lastname: Yeh
mesh_terms: D000328:Adult; D001564:Benzo(a)pyrene / Q000008:administration & dosage / Q000378:metabolism*; D001580:Benzopyrenes / Q000032:analysis*; D002273:Carcinogens / Q000032:analysis* / Q000378:metabolism; D002606:Charcoal; D003296:Cooking / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008297:Male; D008460:Meat; D055815:Young Adult
chemical_list: D001580:Benzopyrenes; D002273:Carcinogens; D002606:Charcoal; D001564:Benzo(a)pyrene; C021338:3-hydroxybenzo(a)pyrene