title: [Experimental study of liver injury in rats of abdominal infection with abdominal compartment syndrome].
abstract: OBJECTIVE
  To evaluate the liver injury in rats of abdominal infection complicated with abdominal compartment syndrome(ACS).
  
  
  METHODS
  SD rats were divided into four groups, including the sham group, the abdominal infection group, the ACS group, and the abdominal infection plus ACS group (combination group). Rats were sacrificed at 1 h, 6 h, 12 h, 24 h after operation with 6 rats at each time point. Blood specimens were collected for liver function testing. Liver tissues were assessed by pathologically examination with hepatic injury severity scoring(HISS). The expressions of Toll-like receptor 4 (TLR4),TNF-α and IL-6 were examined by reverse transcription- polymerase chain reaction.
  
  
  RESULTS
  At 24 h after operation, as compared to the sham group(18.2±1.3) U/L and (105.6±25.5) U/L, ALT and AST increased obviously in the abdominal infection group(68.2±17.5) U/L and (184.6±36.1) U/L, the ACS group (305.2±128.2) U/L and (638.0±104.8) U/L and the combination group (409.2±67.1) U/L and (743.2±250.2) U/L, while the combination group had a higher level as compared to the infection group and the ACS group(all P<0.05). HISS scores were significantly higher in the abdominal infection group(5.0), the ACS group(5.5) and the combination group(7.0) as compared to the sham group(1.5), but no significant differences were found among the three groups at 24 h after operation. Expressions of TLR4, TNF-α and IL-6 were significantly higher in combination group than those in the other three groups.
  
  
  CONCLUSIONS
  Liver function can be affected by abdominal infection and ACS. Abdominal infection plus ACS results in more severe liver injury.
authors.affiliation: Research Institute of General Surgery, Jinling Hospital, Nanjing University School of Medcine, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Jian-an
authors.initials: JA
authors.lastname: Ren
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Guo-sheng
authors.initials: GS
authors.lastname: Gu
authors.forename: Chao-gang
authors.initials: CG
authors.lastname: Fan
authors.forename: Xin-bo
authors.initials: XB
authors.lastname: Wang
authors.forename: Jie-shou
authors.initials: JS
authors.lastname: Li
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism; D059325:Intra-Abdominal Hypertension / Q000201:enzymology / Q000503:physiopathology*; D059413:Intraabdominal Infections / Q000201:enzymology / Q000503:physiopathology*; D008099:Liver / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase