title: The Seryl-tRNA synthetase/tRNASer acceptor stem interface is mediated via a specific network of water molecules.
abstract: tRNAs are aminoacylated by the aminoacyl-tRNA synthetases. There are at least 20 natural amino acids, but due to the redundancy of the genetic code, 64 codons on the mRNA. Therefore, there exist tRNA isoacceptors that are aminoacylated with the same amino acid, but differ in their sequence and in the anticodon. tRNA identity elements, which are sequence or structure motifs, assure the amino acid specificity. The Seryl-tRNA synthetase is an enzyme that depends on rather few and simple identity elements in tRNA(Ser). The Seryl-tRNA-synthetase interacts with the tRNA(Ser) acceptor stem, which makes this part of the tRNA a valuable structural element for investigating motifs of the protein-RNA complex. We solved the high resolution crystal structures of two tRNA(Ser) acceptor stem microhelices and investigated their interaction with the Seryl-tRNA-synthetase by superposition experiments. The results presented here show that the amino acid side chains Ser151 and Ser156 of the synthetase are interacting in a very similar way with the RNA backbone of the microhelix and that the involved water molecules have almost identical positions within the tRNA/synthetase interface.
authors.affiliation: Institute of Chemistry and Biochemistry, Free University Berlin, Berlin, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Eichert
authors.forename: Dominik
authors.initials: D
authors.lastname: Oberthuer
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gessner
authors.forename: Volker A
authors.initials: VA
authors.lastname: Erdmann
authors.forename: Jens P
authors.initials: JP
authors.lastname: Fürste
authors.forename: Charlotte
authors.initials: C
authors.lastname: Förster
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012362:RNA, Transfer, Ser / Q000737:chemistry*; D012714:Serine-tRNA Ligase / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D012362:RNA, Transfer, Ser; D014867:Water; D012714:Serine-tRNA Ligase