title: Ligand competition binding assay for the androgen receptor.
abstract: Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.
authors.affiliation: Department of Chemical Biology and Therapeutics, St. Jude Children's Research Hospital, Memphis, TN, USA. clementinefeau@gmail.com
authors.forename: Clémentine
authors.initials: C
authors.lastname: Féau
authors.forename: Leggy A
authors.initials: LA
authors.lastname: Arnold
authors.forename: Aaron
authors.initials: A
authors.lastname: Kosinski
authors.forename: R Kiplin
authors.initials: RK
authors.lastname: Guy
mesh_terms: D001667:Binding, Competitive; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008297:Male; D011869:Radioligand Assay / Q000379:methods*; D011944:Receptors, Androgen / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D008024:Ligands; D011944:Receptors, Androgen; D011994:Recombinant Proteins