title: Gene targeting in the mouse.
abstract: Establishment of methods to inactivate genes by homologous recombination in embryonic stem (ES) cells has provided great advantages to the field of mouse genetics. Using this technology, a number of null mutant mice, so-called knock-out mice, have been generated. The gene-targeting technology offers a strong tool that allows us to understand the function of a particular gene of interest in the whole animal and has contributed to studies in a wide variety of biological research areas. More recently, the original knock-out technology has been further refined to develop advanced strategies to generate conditional knock-out and knock-in mice. In this chapter, an overview of gene-targeting strategies is presented and procedures to generate these genetically engineered mice are discussed.
authors.affiliation: Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, USA. aegriep@wisc.edu
authors.forename: Anne E
authors.initials: AE
authors.lastname: Griep
authors.forename: Manorama C
authors.initials: MC
authors.lastname: John
authors.forename: Sakae
authors.initials: S
authors.lastname: Ikeda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ikeda
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D015925:Cryopreservation; D004247:DNA / Q000235:genetics; D018274:Electroporation; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D055879:Gene Knock-In Techniques; D018390:Gene Targeting / Q000379:methods*; D005838:Genotype; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*
chemical_list: D004247:DNA