title: IC-tagged proteins are able to interact with each other and perform complex reactions when integrated into muNS-derived inclusions.
abstract: We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia y Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), Universidad de Santiago de Compostela, Campus Vida S/N, 15782-Santiago de Compostela (A Coruña), Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Brandariz-Nuñez
authors.forename: Iria
authors.initials: I
authors.lastname: Otero-Romero
authors.forename: Javier
authors.initials: J
authors.lastname: Benavente
authors.forename: Jose M
authors.initials: JM
authors.lastname: Martinez-Costas
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D007150:Immunohistochemistry; D007181:Inclusion Bodies, Viral / Q000737:chemistry / Q000378:metabolism*; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D015336:Molecular Probe Techniques; D030101:Orthoreovirus, Avian / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D014764:Viral Proteins; D000097573:Red Fluorescent Protein