title: Genotoxicity of SPL (spent pot lining) as measured by Tradescantia bioassays.
abstract: Spent Pot Liner (SPL) is a solid waste product generated in the process of aluminum production. Tradescantia micronuclei (Trad-MN) and stamen hair mutation (Trad-SHM) bioassays are very useful tests to assess genotoxicity of environmental pollutants. In the present study, we intended to investigate the genotoxicity of this waste with Tradescantia bioassays using leachates of SPL simulating the natural leachability of SPL in soil. The formation of micronuclei (MN) was found to be concentration dependent. MN frequency enhanced significantly with SPL treatment. In addition, SPL also appeared to increase the percentage of dyads and triads. Trad-SHM assay showed that SPL increases pink mutation events as SPL concentration increases. These results demonstrated that SPL is a cytogenotoxic agent that affects different genetic end-points (induction of micronuclei and point mutations) even at low concentration (2% and 3%).
authors.affiliation: Department of Biology, Federal University of Lavras, Box 3730, 37200-000, Lavras-MG, Brazil. larissa_lavras@yahoo.com.br
authors.forename: L F
authors.initials: LF
authors.lastname: Andrade-Vieira
authors.forename: L C
authors.initials: LC
authors.lastname: Davide
authors.forename: L S
authors.initials: LS
authors.lastname: Gedraite
authors.forename: J M S
authors.initials: JM
authors.lastname: Campos
authors.forename: H
authors.initials: H
authors.lastname: Azevedo
mesh_terms: D000535:Aluminum; D004249:DNA Damage; D018744:DNA, Plant / Q000187:drug effects; D054750:Ecotoxicology / Q000379:methods; D019652:Extraction and Processing Industry; D007220:Industrial Waste / Q000009:adverse effects*; D015162:Micronucleus Tests; D012989:Soil Pollutants / Q000009:adverse effects*; D034823:Tradescantia / Q000187:drug effects* / Q000235:genetics
chemical_list: D018744:DNA, Plant; D007220:Industrial Waste; D012989:Soil Pollutants; D000535:Aluminum