title: Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase from Thermotoga neapolitana strain DSM 4359.
abstract: Crystals of β-1,3-xylanase (1,3-β-D-xylan xylanohydrolase; EC 3.2.1.32) from Thermotoga neapolitana strain DSM 4359 with maximum dimensions of 0.2×0.1×0.02 mm were grown using the sitting-drop vapour-diffusion method at 293 K over 24 h. The crystals diffracted to a resolution of 1.82 Å, allowing structure determination. The crystals belonged to space group P2(1), with unit-cell parameters a=39.061, b=75.828, c=52.140 Å; each asymmetric unit cell contained a single molecule.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Fumiyoshi
authors.initials: F
authors.lastname: Okazaki
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kurebayashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsuruta
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D015870:Gene Expression; D046949:Thermotoga neapolitana / Q000201:enzymology*; D043325:Xylan Endo-1,3-beta-Xylosidase / Q000737:chemistry*
chemical_list: D043325:Xylan Endo-1,3-beta-Xylosidase