title: Coloured semi-reflective thin films for biomass-hydrolyzing enzyme detection.
abstract: A new enzymatic activity detection assay based on colour change of the semi-reflective films is presented. The method is based on the preparation of multilayered thin films of controlled thickness obtained by sequential deposition of cellulose nanocrystals and xyloglucan. The hydrolysis of the films leads to a decrease in layer thickness that enables to detect enzyme activity, to the naked eye, from the resulting colour changes in a span of few minutes. The method allows direct, fast, highly sensitive, and easy-to-use characterization of enzymatic activities.
authors.affiliation: INRA, UR1268 Biopolymères Interactions Assemblages, F-44316 Nantes, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Cerclier
authors.forename: Aurélie
authors.initials: A
authors.lastname: Guyomard-Lack
authors.forename: Céline
authors.initials: C
authors.lastname: Moreau
authors.forename: Fabrice
authors.initials: F
authors.lastname: Cousin
authors.forename: Nadège
authors.initials: N
authors.lastname: Beury
authors.forename: Estelle
authors.initials: E
authors.lastname: Bonnin
authors.forename: Bruno
authors.initials: B
authors.lastname: Jean
authors.forename: Bernard
authors.initials: B
authors.lastname: Cathala
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000032:analysis / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D003124:Colorimetry; D004798:Enzymes / Q000032:analysis* / Q000378:metabolism; D005936:Glucans / Q000737:chemistry; D006868:Hydrolysis; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D014990:Xylans / Q000737:chemistry
chemical_list: D004798:Enzymes; D005936:Glucans; D011108:Polymers; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; D002480:Cellulase