title: Vanillin-coumarin hybrid molecule as an efficient fluorescent probe for trace level determination of Hg(II) and its application in cell imaging.
abstract: An efficient Hg(2+) selective fluorescent probe (vanillin azo coumarin, VAC) was synthesized by blending vanillin with coumarin. VAC and its Hg(2+) complex were well characterized by different spectroscopic techniques like (1)H NMR, QTOF-MS ES(+), FTIR and elemental analysis as well. VAC could detect up to 1.25 μM Hg(2+) in aqueous methanol solution through fluorescence enhancement. The method was linear up to 16 μM of Hg(2+). Negative interferences from Cu(2+), Ni(2+), Fe(3+), and Zn(2+) were eliminated using EDTA as a masking agent. VAC showed a strong binding to Hg(2+) ion as evident from its binding constant value (2.2×10(5)), estimated using Benesi-Hildebrand equation. Mercuration assisted restricted rotation of the vanillin moiety and inhibited photoinduced electron transfer from the O, N-donor sites to the coumarin unit are responsible for the enhancement of fluorescence upon mercuration of VAC. VAC was used for imaging the accumulation of Hg(2+) ions in Candida albicans cells.
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, India.
authors.forename: Subarna
authors.initials: S
authors.lastname: Guha
authors.forename: Sisir
authors.initials: S
authors.lastname: Lohar
authors.forename: Ipsit
authors.initials: I
authors.lastname: Hauli
authors.forename: Subhra K
authors.initials: SK
authors.lastname: Mukhopadhyay
authors.forename: Debasis
authors.initials: D
authors.lastname: Das
mesh_terms: D000465:Algorithms; D001547:Benzaldehydes / Q000737:chemistry*; D002176:Candida albicans / Q000737:chemistry*; D002614:Chelating Agents / Q000494:pharmacology; D003300:Copper / Q000037:antagonists & inhibitors; D003374:Coumarins / Q000737:chemistry*; D004492:Edetic Acid / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000737:chemistry*; D007501:Iron / Q000037:antagonists & inhibitors; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009532:Nickel / Q000037:antagonists & inhibitors; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D015032:Zinc / Q000037:antagonists & inhibitors
chemical_list: D001547:Benzaldehydes; D002614:Chelating Agents; D003374:Coumarins; D005456:Fluorescent Dyes; D003300:Copper; D009532:Nickel; D004492:Edetic Acid; C030123:coumarin; C100058:vanillin; D007501:Iron; D008628:Mercury; D015032:Zinc