title: Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells.
abstract: Tissue engineering of nerve grafts requires synergistic combination of scaffolds and techniques to promote and direct neurite outgrowth across the lesion for effective nerve regeneration. In this study, we fabricated a composite polymeric scaffold which is conductive in nature by electrospinning and further performed electrical stimulation of nerve stem cells seeded on the electrospun nanofibers. Poly-L-lactide (PLLA) was blended with polyaniline (PANi) at a ratio of 85:15 and electrospun to obtain PLLA/PANi nanofibers with fiber diameters of 195 ± 30 nm. The morphology, chemical and mechanical properties of the electrospun PLLA and PLLA/PANi scaffolds were carried out by scanning electron microscopy (SEM), X-ray photo electron spectroscopy (XPS) and tensile instrument. The electrospun PLLA/PANi fibers showed a conductance of 3 × 10⁻⁹ S by two-point probe measurement. In vitro electrical stimulation of the nerve stem cells cultured on PLLA/PANi scaffolds applied with an electric field of 100 mV/mm for a period of 60 min resulted in extended neurite outgrowth compared to the cells grown on non-stimulated scaffolds. Our studies further strengthen the implication of electrical stimulation of nerve stem cells on conducting polymeric scaffolds towards neurite elongation that could be effective for nerve tissue regeneration.
authors.affiliation: Nanoscience and Nanotechnology Initiative, Health Care and Energy Materials Laboratory, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576. nnimpp@nus.edu.sg
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.forename: Laleh
authors.initials: L
authors.lastname: Ghasemi-Mobarakeh
authors.forename: Guorui
authors.initials: G
authors.lastname: Jin
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry / Q000378:metabolism; D004558:Electric Stimulation; D004563:Electrochemistry / Q000379:methods; D019344:Lactic Acid / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009416:Nerve Regeneration; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D000814:Aniline Compounds; D011091:Polyesters; C416807:polyaniline; D019344:Lactic Acid; C033616:poly(lactide)