title: Cutting Edge: Immunological consequences and trafficking of human regulatory macrophages administered to renal transplant recipients.
abstract: Regulatory macrophages (M regs) were administered to two living-donor renal transplant recipients. Both patients were minimized to low-dose tacrolimus monotherapy within 24 wk of transplantation and subsequently maintained excellent graft function. After central venous administration, most M regs remained viable and were seen to traffic from the pulmonary vasculature via the blood to liver, spleen, and bone marrow. By 1 y posttransplantation, both patients displayed patterns of peripheral blood gene expression converging upon the IOT-RISET signature. Furthermore, both patients maintained levels of peripheral blood FOXP3 and TOAG-1 mRNA expression within the range consistent with nonrejection. It is concluded that M regs warrant further study as a potential immune-conditioning therapy for use in solid-organ transplantation. The results of this work are being used to inform the design of The ONE Study, a multinational clinical trial of immunomodulatory cell therapy in renal transplantation.
authors.affiliation: Laboratory for Transplantation Research, Department of Surgery, University Hospital Regensburg, Regensburg 93053, Germany. james.hutchinson@klinik.uni-regensburg.de
authors.forename: James A
authors.initials: JA
authors.lastname: Hutchinson
authors.forename: Paloma
authors.initials: P
authors.lastname: Riquelme
authors.forename: Birgit
authors.initials: B
authors.lastname: Sawitzki
authors.forename: Stefan
authors.initials: S
authors.lastname: Tomiuk
authors.forename: Patrick
authors.initials: P
authors.lastname: Miqueu
authors.forename: Maaz
authors.initials: M
authors.lastname: Zuhayra
authors.forename: Hans H
authors.initials: HH
authors.lastname: Oberg
authors.forename: Andreas
authors.initials: A
authors.lastname: Pascher
authors.forename: Ulf
authors.initials: U
authors.lastname: Lützen
authors.forename: Uwe
authors.initials: U
authors.lastname: Janssen
authors.forename: Christiane
authors.initials: C
authors.lastname: Broichhausen
authors.forename: Lutz
authors.initials: L
authors.lastname: Renders
authors.forename: Friedrich
authors.initials: F
authors.lastname: Thaiss
authors.forename: Ernst
authors.initials: E
authors.lastname: Scheuermann
authors.forename: Eberhard
authors.initials: E
authors.lastname: Henze
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Volk
authors.forename: Lucienne
authors.initials: L
authors.lastname: Chatenoud
authors.forename: Robert I
authors.initials: RI
authors.lastname: Lechler
authors.forename: Kathryn J
authors.initials: KJ
authors.lastname: Wood
authors.forename: Dieter
authors.initials: D
authors.lastname: Kabelitz
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Schlitt
authors.forename: Edward K
authors.initials: EK
authors.lastname: Geissler
authors.forename: Fred
authors.initials: F
authors.lastname: Fändrich
mesh_terms: D002465:Cell Movement; D002469:Cell Separation; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D016030:Kidney Transplantation / Q000276:immunology*; D008264:Macrophages / Q000166:cytology* / Q000276:immunology / Q000378:metabolism / Q000637:transplantation; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult