title: Characterization of novel glycolipid antigens with an α-galactose epitope in lactobacilli detected with rabbit anti-Lactobacillus antisera and occurrence of antibodies against them in human sera.
abstract: Anti-Lactobacillus johnsonii (LJ) antisera generated by immunization of rabbits with LJ reacted with glyceroglycolipids in LJ, i.e. dihexaosyl diacylglycerol (DH-DG), trihexaosyl DG (TH-DG) and tetrahexaosyl DG (TetH-DG), whose reactivities with antisera increased proportionally with longer carbohydrate chains of glycolipids. Structural analyses of glycolipids from LJ revealed that DH-DG was Galα1-2Glcα1-3'DG, and TH-DG and TetH-DG were novel derivatives of it with α-Gal at the non-reducing terminal, i.e. Galα1-6Galα1-2Glcα1-3'DG and Galα1-6Galα1-6Galα1-2Glcα1-3'DG, respectively. DH-DG was commonly present in several lactobacilli examined, but TetH-DG was restricted to LJ, L. intestinalis and L. reuteri, while the TH-DGs from L. casei were Glc1-6Galα1-2Glcα1-3'DG and an esterified derivative of it, Glc1-6Galα1-2Glc(6-fatty acid)α1-3'DG, as reported in the literature. Anti-LJ antisera reacted with TH-DG and esterified TH-DG from L. casei to lesser extents, but not at all with gentibiosyl DG from Staphylococcus epidermidis or kojibiosyl DG from Streptococcus salivalis or sphingoglycolipids containing α-Gal residues. The major molecular species of glycolipids obtained from lactobacilli were 11-octadecenoic and 11,12-methylene-octadecanoic acids-containing ones. Also, human IgM antibodies against TH-DG and TetH-DG from LJ were detected in human sera, with various antibody titres, indicating that an immune reaction to symbiotic lactobacilli occurs against their glycolipid antigens, TH-DG and TetH-DG.
authors.affiliation: Department of Biochemistry, Kinki University, Kowakae, Higashiosaka, Osaka, Japan. iwamori@life.kindai.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Iwamori
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sakai
authors.forename: Norihito
authors.initials: N
authors.lastname: Minamimoto
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Iwamori
authors.forename: Kyoko
authors.initials: K
authors.lastname: Tanaka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Aoki
authors.forename: Shigeki
authors.initials: S
authors.lastname: Adachi
authors.forename: Taisei
authors.initials: T
authors.lastname: Nomura
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology*; D005690:Galactose / Q000737:chemistry*; D006017:Glycolipids / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007778:Lactobacillus / Q000276:immunology*; D011817:Rabbits
chemical_list: D000907:Antibodies, Bacterial; D000941:Antigens; D000939:Epitopes; D006017:Glycolipids; D007106:Immune Sera; C015903:glycerolglycolipids; D005690:Galactose