title: Novel protein targets for organophosphorus pesticides in rat brain.
abstract: We report preliminary results from a proteomic search for rat brain protein targets adducted by organophosphorous pesticides. Azamethaphos, chlorfenvinphos, diazinon, malathion and chlorpyrifos oxons (in rat brain homogenates) or pirimiphos-methyl (after systemic treatment) were tested at levels producing no more than 30% inhibition of brain acetylcholinesterase. Loss of reactivity with tritiated diisopropylflurophosphate was taken as proof of adduction by the test organophosphate. In addition to acetylcholinesterase other, previously unrecognised, adducted proteins were detected in total brain protein extracts at 30, 32, 41, 71 and 83kDa. Azamethiphos adducted all but the 30 and 32kDa bands, but chlorpyrifos only acetylcholinesterase.
authors.affiliation: MRC Applied Neuroscience Group, School of Biomedical Sciences, University of Nottingham, Queens Medical Centre, Nottingham NG7 2UH, UK.
authors.forename: Andrea
authors.initials: A
authors.lastname: Murray
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Rathbone
authors.forename: David E
authors.initials: DE
authors.lastname: Ray