title: [Synthesis and spectroscopic properties of covalent albumin conjugates of phthalocyanine zincs substituted with metadicarboxyphenoxyl groups].
abstract: Four kinds of covalent serum albumin (BSA or HSA) conjugates of beta-tetrakis[(3,5-dicarboxy)phenoxy]-phthalocyaninatozinc (1) and beta-octakis [(3, 5-dicarboxy) phenoxy]-phthalocyaninatozinc (2) were synthesized by the approach of amide bond. The molar ratio of phthalocyanine to albumin in conjugates were found to be 6 - 7.1. Their absorption spectra were measured in PBS solution. When conjugated to albumin, compound 1 displays more distinct monomer absorption characteristics (with the maximum absorption at 677 nm) than its free form. Compound 2 in albumin framework exists mostly in monomer form, which is beneficial to photodynamic therapy in aqueous solution. Both 2-BSA and 2-HSA show a sharp and intense Q-band at 681 nm and 682 nm with the molar extinction coefficient of 2.01 x 10(5) and 2.05 x 10(5) mol(-1) L cm(-1) respectively. The Q-band absorption spectra and existent state of phthalocyanine 1 or 2 in conjugates were not affected by the pH value of aqueous solution, which are obviously different from that of the corresponding free phthalocyanine.
authors.affiliation: College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China. rpxiao8@yahoo.com.cn
authors.forename: Rong-ping
authors.initials: RP
authors.lastname: Xiao
authors.forename: Jian-dong
authors.initials: JD
authors.lastname: Huang
authors.forename: Han-hui
authors.initials: HH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry*; D054833:Isoindoles; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010778:Photochemotherapy; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D012709:Serum Albumin; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; D012710:Serum Albumin, Bovine