title: Putative role of cellulosomal protease inhibitors in Clostridium cellulovorans based on gene expression and measurement of activities.
abstract: This study is the first to demonstrate the activity of putative cellulosomal protease/peptidase inhibitors (named cyspins) of Clostridium cellulovorans, using the Saccharomyces cerevisiae display system. Cyspins exhibited inhibitory activities against several representative plant proteases. This suggests that these inhibitors protect their microbe and cellulosome from external attack by plant proteases.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Meguro
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Hideo
authors.initials: H
authors.lastname: Miyake
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Tamaru
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D044603:Cellulosomes / Q000378:metabolism*; D046974:Clostridium cellulovorans / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D008954:Models, Biological; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D010957:Plasmids; D011480:Protease Inhibitors / Q000378:metabolism* / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011480:Protease Inhibitors; D010447:Peptide Hydrolases