title: Studies on the antileishmanial properties of the antimicrobial peptides temporin A, B and 1Sa.
abstract: Given the paucity and toxicity of available drugs for leishmaniasis, coupled with the advent of drug resistance, the discovery of new therapies for this neglected tropical disease is recognised as being of the utmost urgency. As such antimicrobial peptides (AMPs) have been proposed as promising compounds against the causative Leishmania species, insect vector-borne protozoan parasites. Here the AMP temporins A, B and 1Sa have been synthesised and screened for activity against Leishmania mexicana insect stage promastigotes and mammalian stage amastigotes, a significant cause of human cutaneous disease. In contrast to previous studies with other species the activity of these AMPs against L. mexicana amastigotes was low. This suggests that amastigotes from different Leishmania species display varying susceptibility to peptides from the temporin family, perhaps indicating differences in their surface structure, the proposed target of these AMPs. In contrast, insect stage L. mexicana promastigotes were sensitive to two of the screened temporins which clearly demonstrates the importance of screening AMPs against both forms of the parasite.
authors.affiliation: Biophysical Sciences Institute, Department of Chemistry and School of Biological and Biomedical Sciences, Durham University, UK.
authors.forename: Frances L
authors.initials: FL
authors.lastname: Chadbourne
authors.forename: Catriona
authors.initials: C
authors.lastname: Raleigh
authors.forename: Hayder Z
authors.initials: HZ
authors.lastname: Ali
authors.forename: Paul W
authors.initials: PW
authors.lastname: Denny
authors.forename: Steven L
authors.initials: SL
authors.lastname: Cobb
mesh_terms: D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D007891:Leishmania / Q000187:drug effects*; D007896:Leishmaniasis / Q000188:drug therapy*; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents; D011506:Proteins; C104501:temporin