title: Implant-assisted meniscal repair in vivo using a chondrocyte-seeded flexible PLGA scaffold.
abstract: A cell-based engineered construct can be used for healing of intractable meniscal lesions. Our aims were to assess the culture conditions (static versus dynamic oscillation) and the healing capacity of the chondrocyte-seeded flexible implants in a heterotopic mouse model. Swine articular chondrocytes were labeled with PKH 26 or DiI dye and seeded onto a flexible PLGA scaffold using dynamic oscillating conditions for 24 h. Half of cell-seeded scaffolds were cultured in the same dynamic conditions, while the remaining scaffolds were cultured statically. After 7 days, scaffolds were placed between swine meniscal discs and were implanted subcutaneously in nude mice for 6 weeks. Additional constructs for assessing in vivo cell tracking were implanted for 12 weeks. Live/dead assays demonstrated labeled chondrocytes attached throughout the scaffold in both culture conditions. DNA measurements showed no significant difference between the culture conditions. A continuous fibro-cartilaginous healing tissue was observed between meniscal discs in all 12 dynamically cultured constructs and 9 of 11 statically cultured ones. There was no evidence of meniscal healing using acellular scaffold as well as in meniscal constructs lacking an implant. Both PKH 26- and DiI-labeled cells were identified along the healing interface. We conclude the chondrocyte-seeded flexible PLGA implants induce healing of meniscal discs in nude mice. Culture conditions after seeding have no apparent effects on healing.
authors.affiliation: Department of Orthopaedic Surgery, Laboratory for Musculoskeletal Tissue Engineering, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Jeong Joon
authors.initials: JJ
authors.lastname: Yoo
authors.forename: David A
authors.initials: DA
authors.lastname: Bichara
authors.forename: Xing
authors.initials: X
authors.lastname: Zhao
authors.forename: Mark A
authors.initials: MA
authors.lastname: Randolph
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Gill
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D019344:Lactic Acid; D051379:Mice; D008819:Mice, Nude; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D000070600:Tibial Meniscus Injuries; D054457:Tissue Scaffolds
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid