title: Prevalence of hemoplasma infection among cattle in the western part of Japan.
abstract: We have examined for hemoplasma infection among cattle in the Hiroshima and Miyazaki prefectures by using a sensitive real-time PCR, with SYBR Green I and with melting curve analysis, which allow to distinguish the two bovine hemoplasma species, Mycoplasma wenyonii and 'Candidatus M. haemobos'. We found 69.4% of 36 cattle in Hiroshima and 93.8% of 32 cattle in Miyazaki infected with either of these two hemoplasma species. High morbidity in western part of Japan may reflect the activity of arthropod vectors for hemoplasma transmission. We also demonstrated neonatal calves less than three months old affected with hemoplasmas without grazing in summer, suggesting a possibility of vertical transmission.
authors.affiliation: Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka 020–8550, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fujihara
authors.forename: Fumina
authors.initials: F
authors.lastname: Sasaoka
authors.forename: Jin
authors.initials: J
authors.lastname: Suzuki
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujihara
authors.forename: Katsufumi
authors.initials: K
authors.lastname: Ooshita
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ano
authors.forename: Ryô
authors.initials: R
authors.lastname: Harasawa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001179:Arthropod Vectors / Q000382:microbiology / Q000502:physiology; D001181:Arthropods; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D005260:Female; D018445:Infectious Disease Transmission, Vertical; D007564:Japan / Q000453:epidemiology; D009174:Mycoplasma / Q000145:classification*; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000662:veterinary