title: Antimutagenic, antigenotoxic and antioxidant activities of phenolic-enriched extracts from Teucrium ramosissimum: combination with their phytochemical composition.
abstract: The evaluation of the mutagenic and antimutagenic actions of extracts obtained from aerial part of Teucrium ramosissimum was assayed using the Salmonella typhimurium assay system. The effect of the same extracts on genotoxicity and SOS response induced by aflatoxin B(1) as well as nitrofurantoin was investigated in a bacterial assay system, i.e., the SOS chromotest with Escherichia coli PQ37. The different extracts showed no mutagenicity when tested with Salmonella typhimurium strains TA100, TA98 and TA1535 either with or without S9 mix. In contrast, our results prove that T. ramosissimum extracts possess antimutagenic effects against sodium azide, aflatoxin B1, benzo[a]pyrene and 4-nitro-o-phenylenediamine. Moreover, the T. ramosissimum tested extracts exhibited no genotoxicity either with or without the external S9 activation mixtures. However, all the extracts significantly decreased the genotoxicity induced by aflatoxin B(1) and nitrofurantoin. The result obtained by the Ames test confirms those of SOS chromotest. Antioxidant capacity of the tested extracts was evaluated using the enzymatic (xanthine/xanthine oxidase assay) and the non enzymatic (NBT/riboflavine, DPPH and ABTS assays) systems. All extracts exhibited high antioxidant activity except the chloroform and the methanol extracts in DPPH and NBT/riboflavine assays respectively. Our results underline the potential of T. ramosissimum to avoid mutations and also its antioxidant potential.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Rue Avicenne, Monastir 5000, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Ben Sghaier
authors.forename: J
authors.initials: J
authors.lastname: Boubaker
authors.forename: I
authors.initials: I
authors.lastname: Skandrani
authors.forename: I
authors.initials: I
authors.lastname: Bouhlel
authors.forename: I
authors.initials: I
authors.lastname: Limem
authors.forename: K
authors.initials: K
authors.lastname: Ghedira
authors.forename: L
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000345:Affinity Labels; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D052160:Benzothiazoles / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013261:Sterols / Q000032:analysis; D013451:Sulfonic Acids / Q000494:pharmacology; D013634:Tannins / Q000032:analysis; D031367:Teucrium / Q000737:chemistry*; D013868:Thioinosine / Q000031:analogs & derivatives / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000345:Affinity Labels; D016587:Antimutagenic Agents; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D009153:Mutagens; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013261:Sterols; D013451:Sulfonic Acids; D013634:Tannins; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D013868:Thioinosine; C004931:1,1-diphenyl-2-picrylhydrazyl; D014969:Xanthine Oxidase; C001789:4-nitrobenzylthioinosine