title: Recognition of immunogold markers in electron micrographs.
abstract: Immunoelectron microscopy is used in cell biological research to study the spatial distribution of intracellular macromolecules at the ultrastructural level. Colloidal gold particles (immunogold markers) are commonly used to localise molecules of interest on ultrathin sections and can be visualised in transmission electron micrographs as dark spots. Quantitative analysis involves detection of the immunogold markers, and is often performed manually or interactively as part of a stereological estimation technique. The method presented in this paper automatically detects and counts immunogold markers, estimating the location, size and type of each marker. It was evaluated on single-labelled as well as double-labelled images showing markers of two different sizes. This is a first step towards automatic analysis of immunoelectron micrographs, enabling a rapid and more complete quantitative analysis than is currently practicable.
authors.affiliation: School of Computing, University of Dundee, Dundee DD1 4HN, UK.
authors.forename: Ruixuan
authors.initials: R
authors.lastname: Wang
authors.forename: Himanshu
authors.initials: H
authors.lastname: Pokhariya
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: McKenna
authors.forename: John
authors.initials: J
authors.lastname: Lucocq
mesh_terms: D000465:Algorithms; D015415:Biomarkers / Q000378:metabolism; D016616:Cryoultramicrotomy; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers