title: Tanghinigenin from seeds of Cerbera manghas L. induces apoptosis in human promyelocytic leukemia HL-60 cells.
abstract: Tanghinigenin, a cardiac glycoside, is isolated from the seeds of Cerbera manghas L. In this study, we demonstrated that tanghinigenin reduced the viability of human promyelocytic leukemia HL-60 cells in a time- and dose-dependent manner, and efficiently induced apoptosis in HL-60 cells as evidenced by the Annexin V/PI binding assay, DNA fragmentation and AO/EB staining studies. In addition, stimulation of HL-60 cells with tanghinigenin induced a series of intracellular events including the activation of caspase-3, -8, and -9, as well as up-regulation of Fas and FasL protein level. Taken together, caspase activation and Fas/FasL interaction was found to be involved in tanghinigenin-induced HL-60 cell apoptosis.
authors.affiliation: Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's Republic of China.
authors.forename: Guo-Fei
authors.initials: GF
authors.lastname: Wang
authors.forename: Yue-Wei
authors.initials: YW
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Cai-Guo
authors.initials: CG
authors.lastname: Huang
authors.forename: Bing-Hua
authors.initials: BH
authors.lastname: Jiao