title: Isolation and identification of a novel polysaccharide-peptide complex with antioxidant, anti-proliferative and hypoglycaemic activities from the abalone mushroom.
abstract: A novel antioxidant polysaccharide-peptide complex LB-1b from the fruiting bodies of the edible abalone mushroom (Pleurotus abalonus) was purified and identified. The structural characteristic of LB-1b was identified by FTIR (Fourier-transform IR), 13C NMR and 1H NMR spectroscopy. LB-1b is a polysaccharide-peptide complex that contains glucose, rhamnose, glucuronic acid and galactose in the molar ratio of 22.4:1:1.7:1.6 and the N-terminal sequence of its peptide moiety has also been determined. The N-terminal amino acid sequence of LB-1b, IPKERKEFQQAQHLK, showed some resemblance to antioxidant enzymes. LB-1b exhibited high antioxidant activity in erythrocyte haemolysis in vitro and the anti-proliferative activity towards hepatoma HepG2 cells and breast cancer MCF7 cells with an IC50 of 24 and 14 μM respectively. LB-1b also demonstrated hypoglycaemic activity in drug-induced diabetic mice and anti-HIV-1 RT (reverse transcriptase) with an IC50 value of 12.5 μM.
authors.affiliation: Department of Microbiology, College of Life Science, Nankai University, Tianjin, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.forename: Jin Cen
authors.initials: JC
authors.lastname: Fang
authors.forename: Jack Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.forename: Chang Rong
authors.initials: CR
authors.lastname: Wang
authors.forename: Ni Ye
authors.initials: NY
authors.lastname: Zhang
authors.forename: Ting Yi
authors.initials: TY
authors.lastname: Wen
authors.forename: Li Yuan
authors.initials: LY
authors.lastname: Qu
authors.forename: Peng Yun
authors.initials: PY
authors.lastname: Lv
authors.forename: Ruili
authors.initials: R
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.forename: Yin Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Xiao Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000737:chemistry*; D020539:Sequence Analysis, Protein
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D005656:Fungal Proteins; D006023:Glycoproteins; D007004:Hypoglycemic Agents