title: Cable pili and the associated 22 kDa adhesin contribute to Burkholderia cenocepacia persistence in vivo.
abstract: BACKGROUND
  Infection by Burkholderia cenocepacia in cystic fibrosis (CF) patients is associated with poor clinical prognosis. Previously, we demonstrated that one of the highly transmissible strains, BC7, expresses cable pili and the associated 22 kDa adhesin, both of which contribute to BC7 binding to airway epithelial cells. However, the contribution of these factors to induce inflammation and bacterial persistence in vivo is not known.
  
  
  RESULTS
  Wild-type BC7 stimulated higher IL-8 responses than the BC7 cbl and BC7 adhA mutants in both CF and normal bronchial epithelial cells. To determine the role of cable pili and the associated adhesin, we characterized a mouse model of B. cenocepacia, where BC7 are suspended in Pseudomonas aeruginosa alginate. C57BL/6 mice were infected intratracheally with wild-type BC7 suspended in either alginate or PBS and were monitored for lung bacterial load and inflammation. Mice infected with BC7 suspended in PBS completely cleared the bacteria by 3 days and resolved the inflammation. In contrast, mice infected with BC7 suspended in alginate showed persistence of bacteria and moderate lung inflammation up to 5 days post-infection. Using this model, mice infected with the BC7 cbl and BC7 adhA mutants showed lower bacterial loads and mild inflammation compared to mice infected with wild-type BC7. Complementation of the BC7 cblS mutation in trans restored the capacity of this strain to persist in vivo. Immunolocalization of bacteria revealed wild-type BC7 in both airway lumen and alveoli, while the BC7 cbl and BC7 adhA mutants were found mainly in airway lumen and peribronchiolar region.
  
  
  CONCLUSIONS
  B. cenocepacia suspended in alginate can be used to determine the capacity of bacteria to persist and cause lung inflammation in normal mice. Both cable pili and adhesin contribute to BC7-stimulated IL-8 response in vitro, and BC7 persistence and resultant inflammation in vivo.
authors.affiliation: Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia, United States of America.
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
authors.forename: Shyamala
authors.initials: S
authors.lastname: Ganesan
authors.forename: Adam T
authors.initials: AT
authors.lastname: Comstock
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Uma S
authors.initials: US
authors.lastname: Sajjan
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D000464:Alginates / Q000494:pharmacology; D000818:Animals; D019121:Burkholderia Infections / Q000150:complications / Q000382:microbiology / Q000473:pathology; D057508:Burkholderia cenocepacia / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000378:metabolism*; D005798:Genes, Bacterial / Q000235:genetics; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009154:Mutation / Q000235:genetics; D020556:Neutrophil Infiltration / Q000187:drug effects; D011014:Pneumonia / Q000150:complications / Q000382:microbiology / Q000473:pathology
chemical_list: D018829:Adhesins, Bacterial; D000464:Alginates; D006603:Hexuronic Acids; D016209:Interleukin-8; D020723:Glucuronic Acid