title: Evaluation of a multilayered chitosan-hydroxy-apatite porous composite enriched with fibronectin or an in vitro-generated bone-like extracellular matrix on proliferation and diferentiation of osteoblasts.
abstract: The use of extracellular matrix (ECM) molecules from tissues is an interesting way to induce specific responses of cells grown onto composite scaffolds to promote adhesion, proliferation and differentiation. There have been several studies on the effects on cell proliferation and differentiation of osteoprogenitor cells cultured onto composites, either adding some ECM molecules or grown in the presence of growth factors. Other studies involve the use of osteoblasts cultured on a three-dimensional (3D) matrix, enriched with ECM molecules produced by the same cells grown previously inside the composite. Here, the effect of enrichment of a novel multilayered chitosan-hydroxyapatite composite with ECM molecules produced by osteoblasts, or the addition of 25 or 50 µg/ml fibronectin to the composite, on proliferation and differentiation of osteoblasts cultured on these composites was studied. The results showed an increase in the number of osteoblasts from day 1 of culture, which was higher in the group grown onto composites enriched with the highest concentration of fibronectin or with ECM molecules produced naturally by osteoblasts cultured previously on them, when compared with the control group. However, this increment tended to decline in all groups after day 7 of culture, the day when they reached the highest peak of proliferation. Differentiation expressed as alkaline phosphatase activity followed the proliferation pattern of the cells cultivated on the scaffolds. The results demonstrate the potential offered by these enriched 3D multilayered composites for improving their ability as bone grafting material.
authors.affiliation: Faculty of Veterinary and Animal Science, University of Chile, Santiago, Chile. sofernan@uchile.cl
authors.forename: M S
authors.initials: MS
authors.lastname: Fernández
authors.forename: J I
authors.initials: JI
authors.lastname: Arias
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
authors.forename: L
authors.initials: L
authors.lastname: Saenz
authors.forename: A
authors.initials: A
authors.lastname: Neira-Carrillo
authors.forename: M
authors.initials: M
authors.lastname: Yazdani-Pedram
authors.forename: J L
authors.initials: JL
authors.lastname: Arias
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D017886:Durapatite / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D005353:Fibronectins; D048271:Chitosan; D017886:Durapatite; D000469:Alkaline Phosphatase