title: Phenolic composition and biological activities of Juniperus drupacea Labill. berries from Turkey.
abstract: The present study was designed to define the phenolic profile and the biological potential of berries methanol extract of Juniperus drupacea Labill. from Turkey. The total phenolic content (Folin-Ciocalteau assay) was 48.06±0.99mgGAE/g extract. The HPLC-DAD-ESI-MS analysis allowed the determination of the complete phenolic profile of J. drupacea berries. Phenolic acids represented more than 60% of the total phenolics, and tyrosol was the major one (1324±0.64μg/g extract); within the flavonoids amentoflavone was detected as the main constituent (927±0.35μg/g extract). The extract exhibited good antioxidant properties, as determined by different in vitro models: DPPH test (IC(50) 0.38±0.02mg/mL), reducing power (12.63±0.14ASE/mL), Fe(2+) chelating ability (IC(50) 2.26±0.06mg/mL), and TBA test (IC(50) 2.47±1.13μg/mL). Cytotoxicity against Artemia salina was highlighted (LC(50) 489.47±27.8μg/mL), and a significant decrease (p⩽0.05; p⩽0.01) in HepG2 cells viability was observed at the higher concentrations (5-10μg/mL). The extract displayed good antibacterial activity towards Gram-positive bacteria and in particular Staphylococcus aureus was the most susceptible strain (MIC 78.12μg/mL).
authors.affiliation: Pharmaco-Biological Department, University of Messina, Vill. SS. Annunziata, 98168 Messina, Italy. nmiceli@pharma.unime.it
authors.forename: N
authors.initials: N
authors.lastname: Miceli
authors.forename: A
authors.initials: A
authors.lastname: Trovato
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.forename: V
authors.initials: V
authors.lastname: Bellinghieri
authors.forename: A
authors.initials: A
authors.lastname: Melchini
authors.forename: P
authors.initials: P
authors.lastname: Dugo
authors.forename: F
authors.initials: F
authors.lastname: Cacciola
authors.forename: P
authors.initials: P
authors.lastname: Donato
authors.forename: L
authors.initials: L
authors.lastname: Mondello
authors.forename: A
authors.initials: A
authors.lastname: Güvenç
authors.forename: R
authors.initials: R
authors.lastname: De Pasquale
authors.forename: M F
authors.initials: MF
authors.lastname: Taviano
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001156:Artemia; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D005638:Fruit / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018653:Juniperus / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis* / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014421:Turkey
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D017392:Thiobarbituric Acid Reactive Substances; C004931:1,1-diphenyl-2-picrylhydrazyl