title: Membrane perturbation elicits an IRF3-dependent, interferon-independent antiviral response.
abstract: We previously found that enveloped virus binding and penetration are necessary to initiate an interferon-independent, IRF3-mediated antiviral response. To investigate whether membrane perturbations that accompany membrane fusion-dependent enveloped-virus entry are necessary and sufficient for antiviral-state induction, we utilized a reovirus fusion-associated small transmembrane (FAST) protein. Membrane disturbances during FAST protein-mediated fusion, in the absence of additional innate immune response triggers, are sufficient to elicit interferon-stimulated gene induction and establishment of an antiviral state. Using sensors of membrane disruption to activate an IRF3-dependent, interferon-independent antiviral state may provide cells with a rapid, broad-spectrum innate immune response to enveloped-virus infections.
authors.affiliation: Department of Biochemistry and Biomedical Sciences, Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
authors.forename: Ryan S
authors.initials: RS
authors.lastname: Noyce
authors.forename: Kathryne
authors.initials: K
authors.lastname: Taylor
authors.forename: Marta
authors.initials: M
authors.lastname: Ciechonska
authors.forename: Susan E
authors.initials: SE
authors.lastname: Collins
authors.forename: Roy
authors.initials: R
authors.lastname: Duncan
authors.forename: Karen L
authors.initials: KL
authors.lastname: Mossman
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology* / Q000821:virology*; D002522:Chlorocebus aethiops; D005786:Gene Expression Regulation; D050838:Interferon Regulatory Factor-3 / Q000378:metabolism*; D012087:Reoviridae / Q000276:immunology*; D014709:Vero Cells; D053586:Virus Internalization
chemical_list: D050838:Interferon Regulatory Factor-3