title: [Study on ribosomal protein gene expression in patients with ulcerative colitis of Pi-asthenic syndrome and Pi-asthenic syndrome].
abstract: OBJECTIVE
  To study differential expression profiles of ribosomal protein (RP) genes in healthy subjects and ulcerative colitis (UC) patients of Pi-asthenic syndrome (PAS) and of dampness-heat syndrome (DHS), thus providing experimental bases for " Pi as the source of qi and blood" theory from the view of protein synthesis.
  
  
  METHODS
  RP genes arrays were made. The mucous membrane of colon was detected in four UC patients of PAS (UC-PAS), four UC patients of DHS (UC-DHS), and four healthy subjects (N), and data analyzed using BRB-TOOL Software Package (3.9). Bioinformatics analyses were conducted in differential genes.
  
  
  RESULTS
  Low-density RP gene chips were successfully produced, including 77 RP genes and two RP like genes (RPL26-like1 and RPL7-like1). There were twelve differential genes between UC (PAS+DHS) and N, all of which were down-regulated genes. There were nineteen differential genes between UC-DHS and N, all of which showed down-regulating tendency. There were three differential genes between UC-PAS and N, all of which were down-regulated genes. There were six differential genes between UC-PAS and UC-DHS, all of which were up-regulated genes. Cluster analysis showed that normal and UC samples of this chip can be classified according to gene expression profiles, and UC-PAS and UC-DHS can be classified by clustering. Various differential genes had a common transcription regulatory factor.
  
  
  CONCLUSIONS
  RP genes arrays were successfully produced. RP gene expressions were down-regulated in UC-PAS and UC-DHS. Corresponding gene expression profiles were shown in N, UC-PAS and UC-DHS.
authors.affiliation: Institute of Gastroenterology, Guangzhou University of Traditional Chinese Medicine, Guangzhou.
authors.forename: Yu-long
authors.initials: YL
authors.lastname: Chen
authors.forename: Wei-wen
authors.initials: WW
authors.lastname: Chen
authors.forename: Ru-liu
authors.initials: RL
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D055815:Young Adult
chemical_list: D012269:Ribosomal Proteins