title: Analysis of the variability of human normal urine by 2D-GE reveals a "public" and a "private" proteome.
abstract: The characterization of the normal urinary proteome is steadily progressing and represents a major interest in the assessment of clinical urinary biomarkers. To estimate quantitatively the variability of the normal urinary proteome, urines of 20 healthy people were collected. We first evaluated the impact of the sample conservation temperature on urine proteome integrity. Keeping the urine sample at RT or at +4°C until storage at -80°C seems the best way for long-term storage of samples for 2D-GE analysis. The quantitative variability of the normal urinary proteome was estimated on the 20 urines mapped by 2D-GE. The occurrence of the 910 identified spots was analysed throughout the gels and represented in a virtual 2D gel. Sixteen percent of the spots were found to occur in all samples and 23% occurred in at least 90% of urines. About 13% of the protein spots were present only in 10% or less of the samples, thus representing the most variable part of the normal urinary proteome. Twenty proteins corresponding to a fraction of the fully conserved spots were identified by mass spectrometry. In conclusion, a "public" urinary proteome, common to healthy individuals, seems to coexist with a "private" urinary proteome, which is more specific to each individual.
authors.affiliation: Sysdiag UMR 3145 CNRS Bio-Rad, Cap delta/Parc euromédecine, 1682 rue de la Valsière CS 61003, Montpellier, France. franck.molina@sysdiag.cnrs.fr
authors.forename: Laurence
authors.initials: L
authors.lastname: Molina
authors.forename: Nicolas
authors.initials: N
authors.lastname: Salvetat
authors.forename: Randa Ben
authors.initials: RB
authors.lastname: Ameur
authors.forename: Sabine
authors.initials: S
authors.lastname: Peres
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sommerer
authors.forename: Fayçal
authors.initials: F
authors.lastname: Jarraya
authors.forename: Hammadi
authors.initials: H
authors.lastname: Ayadi
authors.forename: Franck
authors.initials: F
authors.lastname: Molina
authors.forename: Claude
authors.initials: C
authors.lastname: Granier
mesh_terms: D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D012016:Reference Values; D014556:Urine / Q000737:chemistry*
chemical_list: D011506:Proteins; D020543:Proteome; C035737:major urinary proteins