title: Recombinant protein production by in vivo polymer inclusion display.
abstract: A novel approach to produce purified recombinant proteins was established. The target protein is produced as polyhydroxyalkanoate (PHA) synthase fusion protein, which mediates intracellular formation of PHA inclusions displaying the target protein. After isolation of the PHA inclusions, the pure target protein was released by simple enterokinase digestion.
authors.affiliation: Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
authors.forename: Katrin
authors.initials: K
authors.lastname: Grage
authors.forename: Verena
authors.initials: V
authors.lastname: Peters
authors.forename: Bernd H A
authors.initials: BH
authors.lastname: Rehm
mesh_terms: D000217:Acyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods; D004765:Enteropeptidase / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D054813:Polyhydroxyalkanoates; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase; D004765:Enteropeptidase