title: Rice 14-3-3 protein (GF14e) negatively affects cell death and disease resistance.
abstract: Plant 14-3-3 proteins regulate important cellular processes, including plant immune responses, through protein-protein interactions with a wide range of target proteins. In rice (Oryza sativa), the GF14e gene, which encodes a 14-3-3 protein, is induced during effector-triggered immunity (ETI) associated with pathogens such as Xanthomonas oryzae pv. oryzae (Xoo). To determine whether the GF14e gene plays a direct role in resistance to disease in rice, we suppressed its expression by RNAi silencing. GF14e suppression was correlated with the appearance of a lesion-mimic (LM) phenotype in the transgenic plants at 3 weeks after sowing. This indicates inappropriate regulation of cell death, a phenotype that is frequently associated with enhanced resistance to pathogens. GF14e-silenced rice plants showed high levels of resistance to a virulent strain of Xoo compared with plants that were not silenced. Enhanced resistance was correlated with GF14e silencing prior to and after development of the LM phenotype, higher basal expression of a defense response peroxidase gene (POX22.3), and accumulation of reactive oxygen species (ROS). In addition, GF14e-silenced plants also exhibit enhanced resistance to the necrotrophic fungal pathogen Rhizoctonia solani. Together, our findings suggest that GF14e negatively affects the induction of plant defense response genes, cell death and broad-spectrum resistance in rice.
authors.affiliation: Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO 80523-1177, USA.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Manosalva
authors.forename: Myron
authors.initials: M
authors.lastname: Bruce
authors.forename: Jan E
authors.initials: JE
authors.lastname: Leach
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016923:Cell Death; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D057865:Plant Immunity; D010940:Plant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D012232:Rhizoctonia / Q000276:immunology / Q000472:pathogenicity; D013997:Time Factors; D014974:Xanthomonas / Q000276:immunology / Q000472:pathogenicity
chemical_list: D048948:14-3-3 Proteins; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D009195:Peroxidase