title: [A series of excellent strategy to analyze environmental sample archaeal diversity].
abstract: OBJECTIVE
  Finding a series of archaeal 16S rRNA gene universal primer applied strategies to detect complex microbial diversity in environmental samples, especially with rapidly development of next generation sequencing technology challenge.
  
  
  METHODS
  We used Oligocheck soft to simulate two pairs of archaeal 16S rRNA gene universal primers with RDP (Ribosomal database project) database 16S rRNA gene sequences matching percentage. In succession, the sediment sample was constructed for two clone libraries by using two pairs of archaeal 16S rRNA gene universal primers.
  
  
  RESULTS
  The soft simulation matched percentage result suggests that primer f109/r958 is better than primer f21/r958. This result is consistent with RFLP and diversity index analyses by two clone libraries.
  
  
  CONCLUSIONS
  Multi-primers and properly primer are used to recovery environmental microbiology diversity, which will be advanced in environmental microbial resolution.
authors.affiliation: Key Lab of Marginal Sea Geology, South China Sea Institute and Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510301, China. wsw@scsio.ac.cn
authors.forename: Guohui
authors.initials: G
authors.lastname: Liu
authors.forename: Houbo
authors.initials: H
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Yan
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D019641:DNA, Archaeal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D019641:DNA, Archaeal; D012336:RNA, Ribosomal, 16S