title: In vivo video rate multiphoton microscopy imaging of human skin.
abstract: We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels × 256 pixels resolution without the use of exogenous contrast agents. Imaging at fast frame rates is critical to reducing image blurring due to patient motion and to providing practically short clinical measurement times. Second harmonic generation and two-photon fluorescence images and videos acquired at optimized wavelengths are presented showing cellular and tissue structures from the skin surface down to the reticular dermis.
authors.affiliation: Integrative Oncology Department-Imaging Unit, British Columbia Cancer Agency Research Centre, Vancouver, British Columbia V5Z 1L3, Canada.
authors.forename: Anthony M D
authors.initials: AM
authors.lastname: Lee
authors.forename: Hequn
authors.initials: H
authors.lastname: Wang
authors.forename: Yingqiu
authors.initials: Y
authors.lastname: Yu
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhao
authors.forename: Harvey
authors.initials: H
authors.lastname: Lui
authors.forename: David I
authors.initials: DI
authors.lastname: McLean
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000078404:Epidermal Cells; D005260:Female; D006801:Humans; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008875:Middle Aged; D017785:Photons; D011786:Quality Control; D012867:Skin / Q000166:cytology*