title: Protective effect of kombucha tea against tertiary butyl hydroperoxide induced cytotoxicity and cell death in murine hepatocytes.
abstract: Kombucha (KT), a fermented black tea (BT), is known to have many beneficial properties. In the present study, antioxidant property of KT has been investigated against tertiary butyl hydroperoxide (TBHP) induced cytotoxicity using murine hepatocytes. TBHP, a reactive oxygen species inducer, causes oxidative stress resulting in organ pathophysiology. Exposure to TBHP caused a reduction in cell viability, increased membrane leakage and disturbed the intra-cellular antioxidant machineries in hepatocytes. TBHP exposure disrupted mitochondrial membrane potential and induced apoptosis as evidenced by flow cytometric analyses. KT treatment, however, counteracted the changes in mitochondrial membrane potential and prevented apoptotic cell death of the hepatocytes. BT treatment also reverted TBHP induced hepatotoxicity, however KT was found to be more efficient. This may be due to the formation of antioxidant molecules like D-saccharic acid-1,4-lactone (DSL) during fermentation process and are absent in BT. Moreover, the radical scavenging activities of KT were found to be higher than BT. Results of the study showed that KT has the potential to ameliorate TBHP induced oxidative insult and cell death in murine hepatocytes more effectively than BT.
authors.affiliation: Department of Life Sciences and Biotechnology, Jadavpur University, 188 Raja S C Mullick Road, Kolkata 700 032, India.
authors.forename: Semantee
authors.initials: S
authors.lastname: Bhattacharya
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Manna
authors.forename: Ratan
authors.initials: R
authors.lastname: Gachhui
authors.forename: Parames C
authors.initials: PC
authors.lastname: Sil
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005285:Fermentation; D005419:Flavonoids / Q000302:isolation & purification; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification; D028321:Plant Preparations / Q000494:pharmacology*; D059808:Polyphenols; D020011:Protective Agents / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry / Q000382:microbiology; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D010636:Phenols; D028321:Plant Preparations; D059808:Polyphenols; D020011:Protective Agents; D013662:Tea; D020122:tert-Butylhydroperoxide