title: Formulation and pharmacokinetic evaluation of a paclitaxel nanosuspension for intravenous delivery.
abstract: Paclitaxel is a diterpenoid isolated from Taxus brevifolia. It is effective for various cancers, especially ovarian and breast cancer. Due to its aqueous insolubility, it is administered dissolved in ethanol and Cremophor EL (BASF, Ludwigshafen, Germany), which can cause serious allergic reactions. In order to eliminate Cremophor EL, paclitaxel was formulated as a nanosuspension by high-pressure homogenization. The nanosuspension was lyophilized to obtain the dry paclitaxel nanoparticles (average size, 214.4 ± 15.03 nm), which enhanced both the physical and chemical stability of paclitaxel nanoparticles. Paclitaxel dissolution was also enhanced by the nanosuspension. Differential scanning calorimetry showed that the crystallinity of paclitaxel was preserved during the high-pressure homogenization process. The pharmacokinetics and tissue distribution of paclitaxel were compared after intravenous administration of paclitaxel nanosuspension and paclitaxel injection. In rat plasma, paclitaxel nanosuspension exhibited a significantly (P < 0.01) reduced area under the concentration curve (AUC)(0-∞) (20.343 ± 9.119 μg · h · mL(-1) vs 5.196 ± 1.426 μg · h · mL(-1)), greater clearance (2.050 ± 0.616 L · kg(-1) · h(-1) vs 0.556 ± 0.190 L · kg(-1) · h(-1)), and shorter elimination half-life (5.646 ± 2.941 vs 3.774 ± 1.352 hours) compared with the paclitaxel solution. In contrast, the paclitaxel nanosuspension resulted in a significantly greater AUC(0-∞) in liver, lung, and spleen (all P < 0.01), but not in heart or kidney.
authors.affiliation: College of Pharmacy, Nanjing University of Technology, Nanjing, People's Republic of China.
authors.forename: Yonglu
authors.initials: Y
authors.lastname: Wang
authors.forename: Xueming
authors.initials: X
authors.lastname: Li
authors.forename: Liyao
authors.initials: L
authors.lastname: Wang
authors.forename: Yuanlong
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002152:Calorimetry, Differential Scanning; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D004355:Drug Stability; D005260:Female; D005612:Freeze Drying; D006207:Half-Life; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D017239:Paclitaxel / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D010316:Particle Size; D011312:Pressure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013501:Surface-Active Agents; D013535:Suspensions / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D004337:Drug Carriers; D013501:Surface-Active Agents; D013535:Suspensions; D017239:Paclitaxel