title: [Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats].
abstract: OBJECTIVE
  To study the quality and genetic diversity in Gentiana macrophylla of different habitats for controlling the quality of Gentiana macrophylla.
  
  
  METHODS
  Main characteristics and microscopic identification were adapted to identify Gentiana macrophylla; HPLC method was applied for analysising the contents of Gentiana macrophylla. RAPD method was applied to study genetic structure and genetic diversity of Gentiana macrophylla.
  
  
  RESULTS
  The experiment showed that the Gentiana macrophylla of different habitats have different main charactericstics, microscopic identifications and contents, and effective ingredient contents of gentiopicroside. The levels of population genetic diversity of various groups followed by HUANGZHONG County population (K) > HUAN County population (J) > ZiWU Mountain population (H).
  
  
  CONCLUSIONS
  The study of external morphology, internal structure, active ingredient content and genetic level of Gentiana macrophylla from different areas provides a scientific basis for protecting wild species and ensuring the quality in introducing and cultivating Gentiana macrophylla.
authors.affiliation: Gansu College of TCM, Lanzhou 730000, China. yhw67@126.com
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Wu
authors.initials: W
authors.lastname: Di
authors.forename: Zhi-jia
authors.initials: ZJ
authors.lastname: Cui
authors.forename: Yue-feng
authors.initials: YF
authors.lastname: Li
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D014644:Genetic Variation; D030012:Gentiana / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D057888:Iridoid Glucosides / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry / Q000235:genetics; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D011786:Quality Control; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods; D013045:Species Specificity
chemical_list: D057888:Iridoid Glucosides; C012997:gentiopicroside