title: [Simultaneous determination of 5 kinds of alkaloids in Kechuanning tablets by SPE-UPLC under different UV-vis wavelength].
abstract: The paper is to establish a method for simultaneous determination of 5 kinds of alkaloids in ephedra and poppy which are in Kechuanning tablets. Solid-phase extraction (SPE) was adopted in pretreatment, and a UPLC method with 2 different wavelengths had been developed: 210 nm for the detection of morphine, codeine phosphate, ephedrine hydrochloride and pseudoephedrine hydrochloride, and 251 nm for papaverine hydrochloride. The column used was Acquity UPLC BEH C18 (100 mm x 2.1 mm ID, 1.7 microm) with linear gradient elution using acetonitrile and 0.1% phosphoric acid. The flow rate was 0.4 mL.min-1, and the column temperature was 30 degrees C. The linear response range was 0.375 0 - 12.50 microg.mL-1 for morphine, 0.064 32 - 2.144 microg.mL-1 for codeine phosphate, 0.030 06 - 1.002 microg.mL-1 for papaverine hydrochloride, 1.126 - 37.52 microg.mL-1 for ephedrine hydrochloride, 0.287 8 - 9.592 microg.mL-1 for pseudoephedrine hydrochloride (r = 0.999 7). The average recoveries of these compounds were 99.26%, 100.6%, 95.29%, 100.1% and 97.48%, respectively. This is a more reasonable and credible method of quality control for Kechuanning tablets.
authors.affiliation: Hebei Provincial Institute for Drug Control, Shijiazhuang 050011, China.
authors.forename: Yong-li
authors.initials: YL
authors.lastname: Liu
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D029789:Ephedra / Q000737:chemistry*; D004809:Ephedrine / Q000032:analysis; D009020:Morphine / Q000032:analysis; D010207:Papaver / Q000737:chemistry*; D010208:Papaverine / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry; D054199:Pseudoephedrine / Q000032:analysis; D011786:Quality Control; D052616:Solid Phase Extraction; D013607:Tablets
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; D013607:Tablets; D009020:Morphine; D054199:Pseudoephedrine; D010208:Papaverine; D004809:Ephedrine; D003061:Codeine