title: Establishment of an immortalized human endometrial stromal cell line with functional responses to ovarian stimuli.
abstract: Studies on the mechanisms of decidualization and endometriosis are often hampered by lack of primary endometrial cells. To facilitate in vitro studies, we established a human endometrial stromal cell line, KC02-44D, immortalized with human telomerase reverse transcriptase. Upon exposure to ovarian stimuli, KC02-44D cells showed similar cytoskeletal marker or gene expression and biochemical phenotype to primary endometrial stromal cells. KC02-44D would be useful for studies of human endometrial function and its associated pathologies.
authors.affiliation: Kamakura Research Laboratories, Chugai Pharmaceutical Co, Ltd, Kamakura, Kanagawa, Japan. yuukimnh@chugai-pharm.co.jp
authors.forename: Munehiro
authors.initials: M
authors.lastname: Yuhki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kajitani
authors.forename: Takakazu
authors.initials: T
authors.lastname: Mizuno
authors.forename: Yuko
authors.initials: Y
authors.lastname: Aoki
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Maruyama
mesh_terms: D003994:Bucladesine / Q000494:pharmacology; D018826:CD13 Antigens / Q000378:metabolism; D002460:Cell Line; D003656:Decidua / Q000187:drug effects / Q000502:physiology; D015536:Down-Regulation; D004717:Endometrium / Q000166:cytology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D008875:Middle Aged; D015260:Neprilysin / Q000378:metabolism; D011960:Receptors, Estrogen / Q000096:biosynthesis; D011980:Receptors, Progesterone / Q000096:biosynthesis; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D014746:Vimentin; D004958:Estradiol; D003994:Bucladesine; D017258:Medroxyprogesterone Acetate; D018826:CD13 Antigens; D015260:Neprilysin