title: Two-dimensional hydrophilic interaction/reversed-phase liquid chromatography for the preparative separation of polar and non-polar taxanes.
abstract: BACKGROUND
  Paclitaxel (Taxol®) is one of the best-known compounds of natural origin that has been used in the pharmaceutical industry. It is derived from taxanes extracted from different parts of yew trees (Taxaceae), cell cultures and taxane-producing endophytic fungi. Previous work reported a semi-preparative HPLC for the extraction of Paclitaxel (a non-polar taxane) from Taxus baccata L. and cell culture. In other work, 10-deacetylbaccatin III (a polar taxane) was separated from this medium at a preparative scale.
  
  
  OBJECTIVE
  To introduce a novel on-line two-dimensional heart-cut liquid chromatography using hydrophilic interaction/reversed-phase chromatography for preparative separation of polar and non-polar taxanes.
  
  
  METHODS
  To achieve this goal the system was developed from analytical to a larger preparative scale. In the first dimension of a semi-preparative column, taxanes were separated from polar compounds using a CN column, and in the second dimension the fraction containing taxanes was transferred to a C₁₈-column.
  
  
  RESULTS
  Using this method polar and non-polar taxanes could be separated at analytical and semi-preparative scales.
  
  
  CONCLUSIONS
  Combination of hydrophilic and reversed-phase chromatography in two-dimensional liquid chromatography (2D-LC) systems is a useful method for the separation of compounds with a large polarity range such as taxanes.
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Rezadoost
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D017239:Paclitaxel / Q000737:chemistry / Q000302:isolation & purification; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D043823:Taxoids / Q000737:chemistry / Q000302:isolation & purification*; D020946:Taxus / Q000737:chemistry*
chemical_list: D043823:Taxoids; D017239:Paclitaxel