title: [The verification of a new DNA double-detection diagnostic kit for identifying novel influenza A (H1N1)].
abstract: OBJECTIVE
  To verify a new kit of "universal and novel influenza A (H1N1) virus nucleic acid double-detection methods (PCR-fluorescence probe)".
  
  
  METHODS
  150 cases of throat swab specimens were collected consecutively. After RNA was extracted, the specimens were detected by the verified kit. At the same time, the same specimens were detected by Real-time PCR diagnostic kit from Beijing CDC as the control. The data were analysed by the Kappa in agreement and by McNemar chi2 in difference test.
  
  
  RESULTS
  The consistency rate of the verified kit and the Beijing CDC kit was universal primer M 97.33%, H1N1 98.67% respectively. The Kappa test and McNemar chi2 test showed that two methods had a higher consistency. Compared to the CDC kit, the "false negative rate" and "false-positive rate"of double-check kit were lower.
  
  
  CONCLUSIONS
  The kit of "universal and novel influenza A (H1N1) virus nucleic acid double-detection methods (PCR-fluorescence probe)" from Shanghai Kehua Bio-Engineering Co., Ltd can be used to detect influenza A and novel influenza A (H1N1).
authors.affiliation: The Department of Clinical Medical Laboratory, the 4th People's Hospital of Chengdu, Chengdu 610016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Li-ming
authors.initials: LM
authors.lastname: Liu
authors.forename: Tong-sheng
authors.initials: TS
authors.lastname: Guo
authors.forename: Hai-bin
authors.initials: HB
authors.lastname: Wang
authors.forename: Yong-li
authors.initials: YL
authors.lastname: Li
authors.forename: Da-gang
authors.initials: DG
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Bo-an
authors.initials: BA
authors.lastname: Li
authors.forename: Yuan-li
authors.initials: YL
authors.lastname: Mao
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic