title: Applications of multifunctional magnetic nanoparticles for the enrichment of proteins for PAGE separation.
abstract: This paper describes the applications of multifunctional magnetic nanoparticles (MNPs) for the enrichment of low-abundance proteins for polyacrylamide gel electrophoresis (PAGE) separation. The hemoglobin-functionalized MNPs, named Hb-MNPs, were obtained based on electrostatic interactions and covalent binding between the hemoglobin (Hb) and the MNPs. It was demonstrated that the proteins in human serum were selectively conjugated to Hb-MNPs, which can be used for the selective enrichment of low-abundance proteins. Three and seven kinds of proteins were identified by MS after 1-D and 2-D PAGE, respectively. Comparing with native PAGE without the treatment of MNPs, some proteins were observed, such as human serum amyloid P component (SAP), vitamin D-binding protein, and serine peptidase inhibitor. Because the high concentration of SAP can be considered as a signal for the neurodegeneration of Alzheimer's disease, the present Hb-MNPs-based method was applied to investigate the serum level of SAP for the diagnosis of Alzheimer's disease, and the results are satisfying.
authors.affiliation: College of Chemistry, Beijing Normal University, Beijing, China.
authors.forename: Binjie
authors.initials: B
authors.lastname: Sun
authors.forename: Lingyun
authors.initials: L
authors.lastname: Huang
authors.forename: Na
authors.initials: N
authors.lastname: Na
authors.forename: Dacheng
authors.initials: D
authors.lastname: He
authors.forename: Jin
authors.initials: J
authors.lastname: Ouyang
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D000683:Serum Amyloid P-Component / Q000737:chemistry / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D006454:Hemoglobins; D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles; D000683:Serum Amyloid P-Component