title: Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system.
abstract: The effects of a genetic insulator (chicken cHS4) on transgene expression in a positive feedback system using the GAL4-VP16 activator and luciferase reporter plasmids were examined. The introduction of cHS4 enhanced luciferase expression in mouse liver, indicating that this system would be useful for efficient transgene expression.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ochiai
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D038101:Insulator Elements; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010957:Plasmids; D015534:Trans-Activators / Q000235:genetics; D019076:Transgenes
chemical_list: C070633:Gal-VP16; D015534:Trans-Activators; D004247:DNA; D008156:Luciferases