title: Simultaneous quantification of three iridoids in rat plasma after oral administration of Zhi-zi-chi decoction using LC-MS.
abstract: A sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the simultaneous determination of geniposide, 6α-hydroxygeniposide, and genipin gentiobioside in rat plasma. After the addition of internal standard (I.S.) salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a Kromasil C(18) column (200 mm × 4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2-250 ng/mL for both 6α-hydroxygeniposide and genipin gentiobioside and 2-2000 ng/mL for geniposide, respectively. The lower limit of quantification (LLOQ) was 2 ng/mL for all the analytes. The validated method was successfully applied to the pharmacokinetics study of geniposide, 6α-hydroxygeniposide, and genipin gentiobioside in rats after oral administration of Zhi-zi-chi decoction.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Long
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
authors.forename: Yan-Shuang
authors.initials: YS
authors.lastname: Huo
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Yan
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D032062:Gardenia / Q000737:chemistry*; D039823:Iridoids / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids