title: On the routine use of soft X-rays in macromolecular crystallography. Part V. Molecular replacement and anomalous scattering.
abstract: Currently, about two thirds of all new macromolecular structures are determined by molecular replacement. In general the method works reliably, but it reaches its limits when the search model differs too much from the target structure in terms of coordinate deviations or completeness. Since anomalously scattering substructures are better conserved than the overall structure, these substructures and the corresponding anomalous intensity differences can be utilized to enhance the performance of molecular-replacement approaches. It is demonstrated that the combined and concomitant use of structure-factor amplitudes and anomalous differences constitutes a promising approach to push the limits of molecular replacement and to make more structures amenable to structure solution by this technique.
authors.affiliation: EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
authors.forename: Johan
authors.initials: J
authors.lastname: Unge
authors.forename: Christoph
authors.initials: C
authors.lastname: Mueller-Dieckmann
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: Paul A
authors.initials: PA
authors.lastname: Tucker
authors.forename: Victor S
authors.initials: VS
authors.lastname: Lamzin
authors.forename: Manfred S
authors.initials: MS
authors.lastname: Weiss
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins