title: Decorin and chondroitin-6 sulfate inhibit B16V melanoma cell migration and invasion by cellular acidification.
abstract: In vivo, cells are embedded in an environment generated and maintained by multiple cell-cell and cell-matrix interactions. While transiting the dermis metastasizing melanoma cells interact with the extracellular matrix (ECM) and fibroblasts. To study the roles of ECM components and fibroblasts in melanoma (B16V) cell migration and invasion, we established a co-culture system consisting of fibroblasts, their collagen-rich matrix and B16V cells. The crosstalk between B16V cells and fibroblasts was indicated by a clear increase in release and activity of matrix-metallo-protease-2. Time-lapse microscopy revealed that in B16V cells exposed to either decorin or chondroitin sulfates migration and invasion decreased by more than 50%. Decorin led to a reversible, chondroitin-6-sulfate to an irreversible, cytosolic acidification of B16V cells. Interestingly, decorin lowered NHE1 activity whereas chondroitin-6-sulfate did not. Furthermore, decorin and chondroitin-6-sulfate also acidified the pH at the cell surface which might prevent migration due to strong adhesion. In conclusion, the present co-culture system is an appropriate tool to analyze migration, invasion, and MMP release depending on cell-matrix interactions and the crosstalk between the invasive cells and those surrounded by their self-made matrix. We show a so far unknown function of decorin and chondroitin-6-sulfate: their ability to inhibit B16V cell migration by intracellular acidification.
authors.affiliation: Institute of Physiology II, Münster University, UKM, Münster, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Stock
authors.forename: Oliver
authors.initials: O
authors.lastname: Jungmann
authors.forename: Daniela G
authors.initials: DG
authors.lastname: Seidler
mesh_terms: D000143:Acids / Q000378:metabolism*; D000818:Animals; D002450:Cell Communication / Q000502:physiology; D045744:Cell Line, Tumor; D002464:Cell Migration Inhibition / Q000502:physiology*; D002809:Chondroitin Sulfates / Q000378:metabolism* / Q000502:physiology; D018920:Coculture Techniques; D058575:Decorin / Q000378:metabolism* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D006863:Hydrogen-Ion Concentration; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009361:Neoplasm Invasiveness / Q000473:pathology; D012867:Skin / Q000166:cytology; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D000143:Acids; C546879:Dcn protein, mouse; D058575:Decorin; D002809:Chondroitin Sulfates