title: A novel mycovirus closely related to hypoviruses that infects the plant pathogenic fungus Sclerotinia sclerotiorum.
abstract: Three dsRNA segments, two similarly sized at 9.5kbp and a third one of approximately 3.6kbp, were extracted from a hypovirulent strain SZ-150 of Sclerotinia sclerotiorum. The complete cDNA sequence of one of the two large dsRNA segment (10398bp, excluding the poly (A) tail) reveals a single ORF that encodes a polyprotein with conserved domains of putative papain-like protease, UDP glucose/sterol glycosyltransferase, RNA-dependent RNA polymerase and viral RNA Helicase. This virus is closely related to Cryphonectria hypovirus (CHV) 3/GH2 and CHV4/SR2 in the family Hypoviridae and designated as Sclerotinia sclerotiorum hypovirus 1 (SsHV1/SZ-150). The satellite-like 3.6kbp dsRNA segment (S-dsRNA) shares high sequence identity with the 5'-UTR of SsHV1/SZ-150. SsHV1/SZ-150 alone is not the primary causal agent for hypovirulence of strain SZ-150 since strains without the S-dsRNA show normal phenotype. This is the first report of a naturally occurring hypovirus that infects a fungus other than Cryphonectria parasitica.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, PR China.
authors.forename: Jiatao
authors.initials: J
authors.lastname: Xie
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Xiao
authors.forename: Yanping
authors.initials: Y
authors.lastname: Fu
authors.forename: Huiquan
authors.initials: H
authors.lastname: Liu
authors.forename: Jiasen
authors.initials: J
authors.lastname: Cheng
authors.forename: Said A
authors.initials: SA
authors.lastname: Ghabrial
authors.forename: Guoqing
authors.initials: G
authors.lastname: Li
authors.forename: Daohong
authors.initials: D
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000302:isolation & purification / Q000472:pathogenicity / Q000821:virology*; D001483:Base Sequence; D029688:Brassica napus / Q000382:microbiology; D016000:Cluster Analysis; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins