title: Involvement of nitric oxide (NO) signalling pathway in the antidepressant activity of essential oil of Valeriana wallichii Patchouli alcohol chemotype.
abstract: Valeriana wallichii DC (Valerianaceae), popularly named as Indian valerian has been shown to exist as three chemotypes. The present study evaluated the antidepressant like effect of root essential oil of Valeriana wallichii patchouli alcohol chemotype in both acute and chronic treatment study using forced swim test (FST). Mice (n=6 per group) received 10, 20 and 40 mg/kg p.o. doses of test drug. Single administration of oil significantly inhibited the immobility period (57.6% and 46.9%) at doses 20 and 40 mg/kg respectively without changing the motor function (p<0.05). Similarly, daily administration of essential oil (20mg/kg) for 14 days significantly reduced the immobility period (69.9%) in FST (p<0.05). The neurotransmitter levels in mouse brain were estimated on day 14 after the behavioral study. Significant increase in the level of norepinephrine (29%) and serotonin (19%) (p<0.05) was found at 20mg/kg dose, while no change was observed at 10 and 40 mg/kg doses. The antidepressant-like effect of essential oil (20mg/kg) was prevented by pretreatment of mice with l-arginine (750 mg/kg i.p.) and sildenafil (5mg/kg i.p). On the contrary, pretreatment of mice with l-NAME (10mg/kg i.p.) or methylene blue (10mg/kg i.p.) potentiated the antidepressant action of essential oil (10mg/kg). Taken together, these findings demonstrated that nitric oxide pathway is involved in mediating antidepressant like effect of essential oil from this chemotype.
authors.affiliation: Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India. spilkhwal@yahoo.com
authors.forename: Sangeeta Pilkhwal
authors.initials: SP
authors.lastname: Sah
authors.forename: Chandra Shekhar
authors.initials: CS
authors.lastname: Mathela
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000737:chemistry / Q000494:pharmacology*; D001120:Arginine / Q000494:pharmacology; D001921:Brain / Q000378:metabolism; D004341:Drug Evaluation; D005080:Exercise Test / Q000187:drug effects; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D046353:Immobility Response, Tonic / Q000187:drug effects; D008751:Methylene Blue / Q000494:pharmacology; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D009822:Oils, Volatile / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology; D010938:Plant Oils / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011687:Purines / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000494:pharmacology; D013550:Swimming; D023382:Toxicity Tests, Acute; D014632:Valerian / Q000737:chemistry*
chemical_list: D000928:Antidepressive Agents; D009822:Oils, Volatile; D010879:Piperazines; D010938:Plant Oils; D011687:Purines; D013450:Sulfones; D009569:Nitric Oxide; D012701:Serotonin; D001120:Arginine; D000068677:Sildenafil Citrate; D008751:Methylene Blue; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine