title: Single-cell electrical lysis of erythrocytes detects deficiencies in the cytoskeletal protein network.
abstract: The network of erythrocyte cytoskeletal proteins significantly influences erythrocyte physical and biological properties. Here we show that the kinetics of erythrocyte lysis during exposure to an electric field is sensitively correlated with defects in the cytoskeletal network. Histograms compiled from single-cell electrical lysis data show characteristics of erythrocyte populations that are deficient in a specific cytoskeletal protein, revealing the presence of cell subpopulations.
authors.affiliation: Department of Chemical Engineering, School of Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, Virginia 24061, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Bao
authors.forename: Gayani C
authors.initials: GC
authors.lastname: Kodippili
authors.forename: Katie M
authors.initials: KM
authors.lastname: Giger
authors.forename: Velia M
authors.initials: VM
authors.lastname: Fowler
authors.forename: Philip S
authors.initials: PS
authors.lastname: Low
authors.forename: Chang
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D017487:Ankyrins / Q000737:chemistry / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics; D048429:Cell Size; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000145:classification; D018274:Electroporation / Q000295:instrumentation*; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology / Q000473:pathology; D006461:Hemolysis; D051379:Mice; D008822:Mice, Transgenic; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*; D000099291:Adducins
chemical_list: D017487:Ankyrins; D002148:Calmodulin-Binding Proteins; D003598:Cytoskeletal Proteins; D000099291:Adducins