title: A Preclinical Evaluation of Antrodia camphorata Alcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging.
abstract: This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent, Antrodia camphorata alcohol extract (ACAE), in a mouse model with the advantageous non-invasive in vivo bioluminescence molecular imaging technology. In vitro analyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells. In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation. In vivo bioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.
authors.affiliation: Cancer Center and Department of Radiatioin Oncology, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Jeng-Feng
authors.initials: JF
authors.lastname: Chiou
authors.forename: Alexander T H
authors.initials: AT
authors.lastname: Wu
authors.forename: Wei-Tin
authors.initials: WT
authors.lastname: Wang
authors.forename: Tsu-Hsiang
authors.initials: TH
authors.lastname: Kuo
authors.forename: Juri G
authors.initials: JG
authors.lastname: Gelovani
authors.forename: I-Hsin
authors.initials: IH
authors.lastname: Lin
authors.forename: Chih-Hsiung
authors.initials: CH
authors.lastname: Wu
authors.forename: Wen-Ta
authors.initials: WT
authors.lastname: Chiu
authors.forename: Win-Ping
authors.initials: WP
authors.lastname: Deng