title: Cytotoxic effects of mycotoxin combinations in mammalian kidney cells.
abstract: The cytotoxicity of three Fusarium mycotoxins (beauvericin, deoxynivalenol and T-2 toxin) has been investigated using the NR assay, after 24, 48 and 72h of incubation. The IC(50) values ranged from 6.77 to 11.08, 3.30 to 10.00 and 0.004 to 0.005 for beauvericin, deoxynivalenol and T-2 toxin, respectively. Once the potential interaction has been detected, a quantitative assessment is necessary to ensure and characterize these interactions, that is, each mycotoxin contributes to the toxic effect in accord with its own potency. Combination of mycotoxins was determined in Vero cells after 24, 48 and 72h of exposure. Isobolograms and median effect method of Chou and Talalay were used to assess the nature and quantitative aspects of interaction observed between studied mycotoxins. Median effect analysis was used to calculate the combination index (CI) with values >1 indicating synergism, 1 additive effect, and <1 antagonism. CI values of BEA+DON (1.22-2.74), BEA+T-2 toxin (1.43-5.89), DON+T-2 toxin (3.13-7.62) and BEA+DON+T-2 toxin (1.32-2.68) for 24, 48 and 72h produced antagonistic effects in Vero cells. The highest antagonistic effect in Vero cells was observed with binary DON and T-2 toxin mixture.
authors.affiliation: Laboratori de Toxicologia, Facultat de Farmacia, Universitat de Valencia, Av. Vicent Andres Estelles, 46100 Burjassot, Valencia, Spain. M.Jose.Ruiz@uv.es
authors.forename: María-José
authors.initials: MJ
authors.lastname: Ruiz
authors.forename: Petra
authors.initials: P
authors.lastname: Macáková
authors.forename: Ana
authors.initials: A
authors.lastname: Juan-García
authors.forename: Guillermina
authors.initials: G
authors.lastname: Font
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D047630:Depsipeptides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005562:Formazans / Q000378:metabolism; D006801:Humans; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013605:T-2 Toxin / Q000633:toxicity*; D013778:Tetrazolium Salts / Q000378:metabolism; D014255:Trichothecenes / Q000633:toxicity*; D014709:Vero Cells
chemical_list: D047630:Depsipeptides; D005562:Formazans; D013778:Tetrazolium Salts; D014255:Trichothecenes; C079782:MTT formazan; C004456:beauvericin; D013605:T-2 Toxin; C007262:deoxynivalenol