title: Subtype specific roles of serotonin receptors in the spine formation of cortical neurons in vitro.
abstract: Dendritic spines are postsynaptic structures which are formed from filopodia. We examined roles of serotonin (5-HT) receptors in the spine formation. Embryonic rat cortical neurons were cultured for 10 or 14 days and treated by 5-HT receptor agonists for 24 h. At 11 days in vitro, 5-HT(1A) agonist increased filopodia density, whereas 5-HT(2A/2C) agonist increased the density of puncta and spines. At 15 days in vitro, 5-HT(1A) agonist decreased the density of puncta and spines, whereas 5-HT(2A/2C) agonist decreased filopodia density with increase of spines. In conclusion, the present study shows 5-HT receptors have subtype-specific effects on the spine formation.
authors.affiliation: University of Tsukuba, Graduate School of Comprehensive Human Sciences, 1-1-1 Tennodai, Tsukuba 305-8577, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Chisako
authors.initials: C
authors.lastname: Kanamaru
authors.forename: Akiko
authors.initials: A
authors.lastname: Ohtani
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Kouji
authors.initials: K
authors.lastname: Senzaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Shiga
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000196:embryology*; D049229:Dendritic Spines / Q000187:drug effects / Q000502:physiology*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology; D044402:Receptor, Serotonin, 5-HT2A / Q000502:physiology; D044405:Receptor, Serotonin, 5-HT2C / Q000502:physiology; D011985:Receptors, Serotonin / Q000145:classification* / Q000502:physiology*
chemical_list: D044402:Receptor, Serotonin, 5-HT2A; D044405:Receptor, Serotonin, 5-HT2C; D011985:Receptors, Serotonin; D044282:Receptor, Serotonin, 5-HT1A