title: Characterization and quantification of hydroxycinnamate derivatives in Stevia rebaudiana leaves by LC-MSn.
abstract: Stevia rebaudiana leaves are used as a zero-calorie natural sweetener in a variety of food products in Asian countries, especially in Japan. In this study, the hydroxycinnamate derivatives of S. rebaudiana have been investigated qualitatively and quantitatively by LC-MSn. Twenty-four hydroxycinnamic acid derivatives of quinic and shikimic acid were detected, and 19 of them were successfully characterized to regioisomeric levels; 23 are reported for the first time from this source. These comprise three monocaffeoylquinic acids (Mr 354), seven dicaffeoylquinic acids (Mr 516), one p-coumaroylquinic acid (Mr 338), one feruloylquinic acid (Mr 368), two caffeoyl-feruloylquinic acids (Mr 530), three caffeoylshikimic acids (Mr 336), and two tricaffeoylquinic acids (Mr 678). Cis isomers of di- and tricaffeoylquinic acids were observed as well. Three tricaffeoylquinic acids identified in stevia leaves are reported for the first time in nature. These phenolic compounds identified in stevia might affect the organoleptic properties and add additional beneficial health effects to stevia-based products.
authors.affiliation: School of Engineering and Science, Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
authors.forename: Hande
authors.initials: H
authors.lastname: Karaköse
authors.forename: Rakesh
authors.initials: R
authors.lastname: Jaiswal
authors.forename: Nikolai
authors.initials: N
authors.lastname: Kuhnert
mesh_terms: D002853:Chromatography, Liquid; D003373:Coumaric Acids / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry*; D011801:Quinic Acid; D012765:Shikimic Acid; D036625:Stevia / Q000737:chemistry*; D013549:Sweetening Agents
chemical_list: D003373:Coumaric Acids; D013549:Sweetening Agents; D011801:Quinic Acid; D012765:Shikimic Acid