title: Nearest neighbor analysis of dopamine D1 receptors and Na(+)-K(+)-ATPases in dendritic spines dissected by STED microscopy.
abstract: Protein localization in dendritic spines is the focus of intense investigations within neuroscience. Applications of super-resolution microscopy to dissect nanoscale protein distributions, as shown in this work with dual-color STED, generate spatial correlation coefficients having quite small values. This means that colocalization analysis to some extent looses part of its correlative impact. In this study we thus introduced nearest neighbor analysis to quantify the spatial relations between two important proteins in neurons, the dopamine D1 receptor and Na(+),K(+)-ATPase. The analysis gave new information on how dense the D1 receptor and Na(+),K(+)-ATPase constituting nanoclusters are located both with respect to the homogenous (self to same) and the heterogeneous (same to other) topology. The STED dissected nanoscale topologies provide evidence for both a joint as well as a separated confinement of the D1 receptor and the Na(+),K(+)-ATPase in the postsynaptic areas of dendritic spines. This confined topology may have implications for generation of local sodium gradients and for structural and functional interactions modulating slow synaptic transmission processes.
authors.affiliation: Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Blom
authors.forename: Daniel
authors.initials: D
authors.lastname: Rönnlund
authors.forename: Lena
authors.initials: L
authors.lastname: Scott
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Spicarova
authors.forename: Ville
authors.initials: V
authors.lastname: Rantanen
authors.forename: Jerker
authors.initials: J
authors.lastname: Widengren
authors.forename: Anita
authors.initials: A
authors.lastname: Aperia
authors.forename: Hjalmar
authors.initials: H
authors.lastname: Brismar
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000737:chemistry*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D017348:Microscopy, Electron, Scanning Transmission; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D036103:Nanotechnology / Q000379:methods; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000032:analysis / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000737:chemistry*
chemical_list: D017447:Receptors, Dopamine D1; D000254:Sodium-Potassium-Exchanging ATPase