title: CD4+CD25(high) Treg cells in peripheral blood during remission and exacerbation of allergic asthma in children.
abstract: OBJECTIVE
  To determine the percentage of CD4+CD25(high) Treg cells in peripheral bloodCD4+ T cells of allergic asthmatic children during disease remission and exacerbation.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMC) and serum samples were collected from 6- to 11-year-old children with mild-to-moderate allergic asthma (n = 34)and from healthy controls (n = 15). CD4+CD25(high) T cells in PBMC were detected by flow cytometry. Total and specific IgE in serum were analysed by enzyme-amplified chemiluminescence, and IL-2 was measured by ELISA.
  
  
  RESULTS
  There was no significant difference in CD4+CD25(high) T-cell proportions between asthmatic children in exacerbation and remission as compared with controls.CD4+CD25(high) T-cell percentages were not correlated with total and specific IgE. IL-2 was elevated in both disease remission and exacerbation but did not correlate significantly with CD4+CD25(high) T-cell percentages.
  
  
  CONCLUSIONS
  CD4+CD25(high) T-cell proportion in the peripheral blood of total CD4+T cells is not reduced in children with allergic IgE-mediated asthma and does not differ between disease remission and exacerbation.
authors.affiliation: Department of Immunology, State Research Institute Center for Innovative Medicine, Vilnius, Lithuania. ingrida.pumputiene@gmail.com
authors.forename: Ingrida
authors.initials: I
authors.lastname: Pumputiene
authors.forename: Regina
authors.initials: R
authors.lastname: Emuzyte
authors.forename: Almantas
authors.initials: A
authors.lastname: Siaurys
authors.forename: Vytas
authors.initials: V
authors.lastname: Tamosiunas
authors.forename: Arunas
authors.initials: A
authors.lastname: Valiulis
mesh_terms: D001249:Asthma / Q000097:blood / Q000276:immunology*; D016022:Case-Control Studies; D002648:Child; D018450:Disease Progression; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D008297:Male; D012075:Remission, Spontaneous; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology
chemical_list: D007376:Interleukin-2; D007073:Immunoglobulin E