title: Time-dependent micromechanical responses of breast cancer cells and adjacent fibroblasts to electric treatment.
abstract: Direct-current, low-intensity, electric fields were suggested as a minimally invasive treatment for various cancers. The tumor microenvironment may affect treatment efficacy, albeit it has not generally  been considered when evaluating novel anti-cancer treatments. We evaluate the effects of electric treatment on epithelial, breast-cancer cells, co-cultured with non-cancerous fibroblasts, a simplified model for the tumor-microenvironment. We evaluate changes in morphology, cytoskeleton, and focus on dynamic intracellular structure and mechanics. Multiple-particle tracking was used within living cells to quantify time-dependent structural and mechanical changes. Cancer cells suffer severe cell death and exhibit transient rounding and changes in internal structural and mechanics. Interestingly, treating cancer cells in co-culture with fibroblasts delays and reduces their responses to treatment. Our particle-tracking data indicates a mechanism relating the observed changes in intracellular transport to transient changes in the microtubule network and its motors. In contrast, fibroblasts are only minimally affected by treatment, separately or in co-culture. To conclude, intracellular mechanics reveal time-dependent responses after treatment, unavailable by bulk measurements. This time-dependence could provide a window of opportunity for continued treatment. We demonstrate the importance of evaluating anti-cancer treatments within their microenvironment, which can affect response magnitude and time-course.
authors.affiliation: Technion-Israel Institute of Technology, Haifa 32000, Israel.
authors.forename: Maayan Lia
authors.initials: ML
authors.lastname: Yizraeli
authors.forename: Daphne
authors.initials: D
authors.lastname: Weihs
mesh_terms: D001696:Biomechanical Phenomena; D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D003599:Cytoskeleton / Q000378:metabolism; D004553:Electric Conductivity; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D055595:Mechanical Phenomena; D009361:Neoplasm Invasiveness; D013997:Time Factors; D059016:Tumor Microenvironment