title: Dual-label STED nanoscopy of living cells using photochromism.
abstract: We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.
authors.affiliation: Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. kwillig@gwdg.de
authors.forename: Katrin I
authors.initials: KI
authors.lastname: Willig
authors.forename: Andre C
authors.initials: AC
authors.lastname: Stiel
authors.forename: Tanja
authors.initials: T
authors.lastname: Brakemann
authors.forename: Stefan
authors.initials: S
authors.lastname: Jakobs
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D005456:Fluorescent Dyes / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008027:Light; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D010777:Photochemistry / Q000379:methods*; D014709:Vero Cells
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins