title: Burkholderia pseudomallei-induced cell fusion in U937 macrophages can be inhibited by monoclonal antibodies against host cell surface molecules.
abstract: Burkholderia pseudomallei induces the formation of multinucleated giant cells in cell monolayers. After infection of human macrophage-like U937 cells with B. pseudomallei, addition of monoclonal antibodies against integrin-associated protein (CD47), E-selectin (CD62E), a fusion regulatory protein (CD98), and E-cadherin (CD324) suppressed multinucleated giant cells in a concentration-dependent manner while monoclonal antibodies against other surface molecules did not inhibit fusion despite binding to the cell surface. Flow cytometric analysis showed increased expression of CD47 and CD98, but not CD62E and CD324, upon B. pseudomallei infection. Our data suggest the involvement of specific cellular factors in the process of B. pseudomallei-induced fusion.
authors.affiliation: Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Suparak
authors.forename: Veerachat
authors.initials: V
authors.lastname: Muangsombut
authors.forename: Donporn
authors.initials: D
authors.lastname: Riyapa
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Stevens
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Ganjana
authors.initials: G
authors.lastname: Lertmemongkolchai
authors.forename: Sunee
authors.initials: S
authors.lastname: Korbsrisate
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016957:Burkholderia pseudomallei / Q000276:immunology* / Q000502:physiology; D051928:CD47 Antigen / Q000276:immunology / Q000378:metabolism; D015820:Cadherins / Q000276:immunology / Q000378:metabolism; D002459:Cell Fusion; D019040:E-Selectin / Q000276:immunology / Q000378:metabolism; D027261:Fusion Regulatory Protein-1 / Q000276:immunology / Q000378:metabolism; D015726:Giant Cells / Q000502:physiology*; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D051381:Rats; D020298:U937 Cells
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D051928:CD47 Antigen; C497894:CD47 protein, human; D015820:Cadherins; D019040:E-Selectin; D027261:Fusion Regulatory Protein-1