title: DNA sequence variation of the tobacco cutworm, Spodoptera litura (Lepidoptera: Noctuidae), determined by mitochondrial A+T-rich region and nuclear ITS2 sequences.
abstract: In an effort to gain greater insight into the nature of the population genetic structure of the pest insect Spodoptera litura (Lepidoptera: Noctuidae), tobacco cutworms were collected from six Korean and five Chinese localities, and their mitochondrial A+T-rich region and nuclear internal transcribed spacer 2 (ITS2) were cloned and sequenced. The A+T-rich region and nuclear ITS2 provided a maximum sequence divergence of 2.88 and 1.82%, respectively. Overall, a low level of genetic fixation (F(ST) = 0-0.02965 in the A+T-rich region and 0-0.34491 in ITS2) and no discernible isolated population were noted among most S. litura populations. Along with these results, the absence of genetic variance between Korea and China indicates a profound interrelation of the S. litura populations in the two countries, consistent with the current notion that S. litura has sufficient flight capacity for dispersal.
authors.affiliation: College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Xinlong
authors.initials: X
authors.lastname: Wan
authors.forename: Jianhong
authors.initials: J
authors.lastname: Li
authors.forename: Min Jee
authors.initials: MJ
authors.lastname: Kim
authors.forename: Hae Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.forename: Iksoo
authors.initials: I
authors.lastname: Kim
mesh_terms: D020856:AT Rich Sequence; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D021901:DNA, Intergenic / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D010802:Phylogeny; D018411:Spodoptera / Q000235:genetics*; D014026:Nicotiana / Q000469:parasitology
chemical_list: D021901:DNA, Intergenic; D004272:DNA, Mitochondrial