title: Tea triterpenoidal saponins from the roots of Camellia sinensis have inhibitory effects against alcohol dehydrogenase.
abstract: Ten new polyhydroxyolean-12-ene pentacyclic triterpenoidal saponins, named rogchaponins 1-10, were isolated from the methanolic extract of the roots of Camellia sinensis by a series of chromatographic methods (silica gel flash column and C18 MPLC followed by C18 HPLC). Their structures were established by 1D and 2D-NMR techniques along with IR and HR-TOF-MS. Rogchaponins R4 ( 4) and R5 (5) showed inhibitory activities against yeast alcohol dehydrogenase (ADH) with IC (50) values of 16.1 ± 3.2 and 15.4 ± 3.3 µM, respectively. A 4-methylpyrazole positive control exhibited an IC (50) of 2750 ± 50 µM. However, the saponins showed no inhibitory activity against yeast aldehyde dehydrogenase (ALDH).
authors.affiliation: Department of Chemistry, Kongju National University, Gongju, Republic of Korea.
authors.forename: Titto
authors.initials: T
authors.lastname: Varughese
authors.forename: Md Maniruzzaman
authors.initials: MM
authors.lastname: Manir
authors.forename: Mozahidur
authors.initials: M
authors.lastname: Rahaman
authors.forename: Jeong Kee
authors.initials: JK
authors.lastname: Kim
authors.forename: Byeong-Gon
authors.initials: BG
authors.lastname: Lee
authors.forename: Surk-Sik
authors.initials: SS
authors.lastname: Moon
mesh_terms: D000143:Acids / Q000737:chemistry; D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D028241:Camellia sinensis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000143:Acids; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D012503:Saponins; D014315:Triterpenes; D000426:Alcohol Dehydrogenase