title: B7-H3 over expression in prostate cancer promotes tumor cell progression.
abstract: OBJECTIVE
  We compared B7-H3 expression in benign prostatic hyperplasia and prostate cancer tissue specimens, and determined the effects of low B7-H3 expression on the PC-3 human prostate cancer cell line using RNA interference.
  
  
  METHODS
  B7-H3 expression in prostate specimens was determined by enzyme-linked immunosorbent assay. A PC-3 cell line with low B7-H3 expression was established by RNA interference to investigate the effect of B7-H3 on cell proliferation, adhesion, migration and invasion in vitro.
  
  
  RESULTS
  B7-H3 in tissue samples was significantly higher in the prostate cancer group than in the benign prostatic hyperplasia group (mean±SEM 174.73±56.80 vs 82.69±46.19 ng/gm, p<0.001). B7-H3 expression down-regulated by small interfering RNA decreased cell adhesion to PC-3 fibronectin more than 30%, and migration and Matrigel™ invasion up to 50%. No apparent impact was observed on cell proliferation.
  
  
  CONCLUSIONS
  B7-H3 is aberrantly expressed in prostate cancer. In addition to modulating tumor immunity, B7-H3 may have a novel role in regulating PC-3 cell progression.
authors.affiliation: Department of Urology, First Affiliated Hospital of Soochow University and Institute of Medical Biotechnology, Soochow University, Suzhou, People's Republic of China.
authors.forename: Hexing
authors.initials: H
authors.lastname: Yuan
authors.forename: Xuedong
authors.initials: X
authors.lastname: Wei
authors.forename: Guangbo
authors.initials: G
authors.lastname: Zhang
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianquan
authors.initials: J
authors.lastname: Hou
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics*; D060887:B7 Antigens; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D011971:Receptors, Immunologic / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D015703:Antigens, CD; D060887:B7 Antigens; C423237:CD276 protein, human; D011971:Receptors, Immunologic