title: [The genetical evolution of the full length genes of 5 EV 71 strains from 5 Shenzhen patients with hand-food-mouth disease associated with EV71 infection].
abstract: OBJECTIVE
  To investigate the characteristics of molecular epidemiology and molecular evolution of 5 EV 71 (enterovirus 71, EV71) strains from 5 Shenzhen patients with hand-food-mouth disease associated with EV 71 infection.
  
  
  METHODS
  5 EV 71 strains were isolated, and sequenced to analyzed the full length gene sequences in order to compare nucleotide and amino acid homology with other EV71 strains from other regions and countries as well as previous strains across the world through bioinformatics software.
  
  
  RESULTS
  5 strains of EV 71 belonged to sub-genotype C4 by analysis of nucleotide sequences of VP1 and VP4 of EV 71. The differences of nucleotide and amino acid sequences were much small with nucleotide homology of 93% and amino acid homology of 98% among these 5 strains. A phylogenetic tree analysis indicated that 2008 Shenzhen epidemic strains were the most close to 2004 Shenzhen circulating strains, and also much close to 1998 Shenzhen epidemic strains and 2008 Fuyang Anhui strains. The dead strain was very close to 2008 Fuyang Anhui epidemic strains.
  
  
  CONCLUSIONS
  It can be speculated that this epidemic strains of EV 71 probably originate from the same ancient strain in the history, may from 1998 Shenzhen strain.
authors.affiliation: The Third People's Hospital of Shenzhen, Shenzhen 518020, China.
authors.forename: Wei-long
authors.initials: WL
authors.lastname: Liu
authors.forename: Gui-lin
authors.initials: GL
authors.lastname: Yang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wei
authors.forename: Ming-xia
authors.initials: MX
authors.lastname: Zhang
authors.forename: Xin-chun
authors.initials: XC
authors.lastname: Chen
authors.forename: Ying-xia
authors.initials: YX
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Bo-ping
authors.initials: BP
authors.lastname: Zhou
mesh_terms: D002681:China; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D010802:Phylogeny