title: Application of tissue microarray for atherectomized tissues from peripheral arterial disease.
abstract: It is not easy to apply tissue microarray (TMA) to atherectomized tissues from peripheral arterial disease because of their physical properties. We introduce a new TMA application technique for atherectomized tissues. Using a pre-made plastic TMA cassette and TMA punch device, we successfully made the TMA block containing 40 vertically oriented atherectomized tissue samples from 10 patients. The histogram of surface areas of tissue cores in the TMA showed a bell-shaped distribution, whereas that of conventionally embedded tissues showed wide distribution. This finding suggests that the TMA method might be a better way of vertical embedding than the conventional method. A TMA block prepared by our method enabled a simultaneous evaluation of the histopathology of vertically oriented atherectomized tissues and the correlation between them with intravascular ultrasound image. In addition, this new method might be applied to various tissues in different ways.
authors.affiliation: Department of Pathology, Yonsei University College of Medicine, Seoul, Republic of Korea.
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Hyosup
authors.initials: H
authors.lastname: Shim
authors.forename: Junjeong
authors.initials: J
authors.lastname: Choi
authors.forename: Seong Hwan
authors.initials: SH
authors.lastname: Park
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Song
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Shin
authors.forename: Donghoon
authors.initials: D
authors.lastname: Choi
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D006801:Humans; D016612:Paraffin Embedding / Q000379:methods*; D058729:Peripheral Arterial Disease / Q000378:metabolism / Q000473:pathology*; D058226:Plaque, Atherosclerotic / Q000378:metabolism / Q000473:pathology*; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D015415:Biomarkers