title: Direct analysis of oligosaccharides and alpha hydroxy acids in fruits using electrosonic spray ionization mass spectrometry.
abstract: Electrosonic spray ionization (ESSI) is a derivative technique of electrospray ionization (ESI) for mass spectrometry (MS) in which droplets are charged in the course of sonic spray. In this study, we applied ESSI MS to direct analysis of oligosaccharides and alpha hydroxy acids (AHAs) in fruits. The components were extracted from fruit fleshes by a feasible method prior to ESSI MS analysis, but the fruit juices were analyzed without further pretreatment. The results demonstrate that mainly alkali metal adducts of oligosaccharides are favorably produced in positive ion mode, while deprotonated AHAs and oligosaccharides are produced in negative ion mode. Compared with mass spectra obtained using electrospray droplet impact/secondary ion mass spectrometry (EDI/SIMS), mass spectra using ESSI make the identification of oligosaccharides more straightforward in positive ion mode than in negative ion mode.
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.forename: Caiqiao
authors.initials: C
authors.lastname: Xiong
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhou
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhen
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Yin
authors.initials: Y
authors.lastname: Tang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.forename: Zongxiu
authors.initials: Z
authors.lastname: Nie
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
mesh_terms: D005638:Fruit / Q000737:chemistry*; D006880:Hydroxy Acids / Q000032:analysis*; D007477:Ions / Q000737:chemistry; D008672:Metals, Alkali / Q000737:chemistry; D009844:Oligosaccharides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization; D018629:Spectrometry, Mass, Secondary Ion
chemical_list: D006880:Hydroxy Acids; D007477:Ions; D008672:Metals, Alkali; D009844:Oligosaccharides