title: Sensitive and selective determination of glucose in human serum and urine based on the peroxyoxalate chemiluminescence reaction of a new fluorophore.
abstract: A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H(2)O(2) was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50×10(-6) to 1.75×10(-4) mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10×10(-6) mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan, Iran. Kiomarszargoosh@yahoo.com
authors.forename: Kiomars
authors.initials: K
authors.lastname: Zargoosh
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.forename: Mohammad
authors.initials: M
authors.lastname: Qandalee
authors.forename: Mohammad
authors.initials: M
authors.lastname: Piltan
authors.forename: Loghman
authors.initials: L
authors.lastname: Moradi
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D005663:Furans / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D006029:Glycosuria / Q000175:diagnosis / Q000378:metabolism / Q000652:urine; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements; D008954:Models, Biological; D010070:Oxalates / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013343:Styrenes / Q000737:chemistry; D016482:Urinalysis / Q000379:methods*
chemical_list: D001786:Blood Glucose; D005456:Fluorescent Dyes; D005663:Furans; D010070:Oxalates; D013343:Styrenes; C569552:di(tert-butyl) 2-(tert-butylamino)-5-(2-phenyl-1-ethenyl)-3,4-furandicarboxylate; C044622:peroxyoxalate; D006861:Hydrogen Peroxide; D005947:Glucose