title: Tubby-tagged balancers for the Drosophila X and second chromosomes.
abstract: We generated FM7a and CyO balancer chromosomes bearing a Tubby1 (Tb1) dominant transgene. Flies heterozygous for these FM7a and CyO derivatives exhibit a phenotype undistinguishable from that elicited by the Tb1 mutation associated with the TM6B balancer. We tested two of these Tb-bearing balancers (FM7-TbA and CyO-TbA) for more than 30 generations and found that the Tb1 transgene they carry is stable. Thus, these new Tb-tagged balancers are particularly useful for balancing lethal mutations and distinguish homozygous mutant larvae from their heterozygous siblings.
authors.affiliation: Dipartimento di Biologia e Biotecnologie “Charles Darwin” and Istituto di Biologia e Patologia Molecolari del CNR, Sapienza, Università di Roma, Rome, Italy.
authors.forename: Ramona
authors.initials: R
authors.lastname: Lattao
authors.forename: Silvia
authors.initials: S
authors.lastname: Bonaccorsi
authors.forename: Xiao
authors.initials: X
authors.lastname: Guan
authors.forename: Steven A
authors.initials: SA
authors.lastname: Wasserman
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
mesh_terms: D000818:Animals; D001947:Breeding; D059006:Chromosomes, Insect / Q000235:genetics*; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005818:Genetic Engineering / Q000379:methods*; D007814:Larva / Q000033:anatomy & histology / Q000235:genetics; D008297:Male; D011995:Recombination, Genetic; D019076:Transgenes