title: Tea polyphenol-induced neuron-like differentiation of mouse mesenchymal stem cells.
abstract: Bone marrow mesenchymal stem cells (BMSCs) can be induced to differentiate into neuron-like cells under appropriate conditions often involving toxic reagents that are not applicable for clinical transplantation. The present study investigated whether tea polyphenol (TP), a native nontoxic antioxidant, could induce mouse neuron-like cell differentiation of BMSCs in vitro. BMSCs, dissected from mouse femur bone marrow, were amplified in culture and treated with TP or beta-mercaptoethanol (BME, control). Morphological changes were observed under light microscopy. After 12 h treatment with 50 microg/ml TP or 5 mM BME, most cells differentiated into neuron-like cells exhibiting neuronal morphological characteristics, cellular shrinkage and neurite growth. Immunocytochemistry and reverse transcription (RT)-PCR results demonstrated neuronal marker expression in the induced cells with no glial fibrillary acidic protein expression. Taken together, TP induced mouse BMSCs to differentiate into neuron-like cells in vitro. These findings provide a potential source for the treatment of various neurological diseases.
authors.affiliation: Department of Kidney Transplantation of Nephropathy Center, First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an 710061, Shaanxi, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Wu-Jun
authors.initials: WJ
authors.lastname: Xue
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Xie
authors.forename: Xin-Shun
authors.initials: XS
authors.lastname: Feng
authors.forename: Fu-Quan
authors.initials: FQ
authors.lastname: Huo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology; D013662:Tea / Q000737:chemistry*
chemical_list: D018696:Neuroprotective Agents; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate