title: Werner interacting protein 1 promotes binding of Werner protein to template-primer DNA.
abstract: Werner interacting protein 1 (WRNIP1) that is highly conserved from Escherichia coli to human was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). Here, human WRNIP1 and WRN are shown to bind to template-primer DNA, and WRNIP1, but not WRN, requires ATP for DNA binding. Under conditions of a limiting amount of WRN, WRNIP1 facilitated binding of WRN to DNA in a dose-dependent manner. However, WRNIP1 did not stimulate the DNA helicase activity of WRN, and WRN displaced pre-bound WRNIP1 from DNA. Functional relationships between WRNIP1 and WRN will be discussed.
authors.affiliation: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980–8578, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kanamori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Seki
authors.forename: Akari
authors.initials: A
authors.lastname: Yoshimura
authors.forename: Toshiki
authors.initials: T
authors.lastname: Tsurimoto
authors.forename: Shusuke
authors.initials: S
authors.lastname: Tada
authors.forename: Takemi
authors.initials: T
authors.lastname: Enomoto
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000255:Adenosine Triphosphate / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D053484:RecQ Helicases / Q000378:metabolism*; D014898:Werner Syndrome / Q000235:genetics* / Q000378:metabolism; D000071657:Werner Syndrome Helicase
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D000255:Adenosine Triphosphate; D004247:DNA; D005090:Exodeoxyribonucleases; C430237:WRNIP1 protein, human; D000074183:ATPases Associated with Diverse Cellular Activities; D053484:RecQ Helicases; C098785:WRN protein, human; D000071657:Werner Syndrome Helicase