title: Subchronic toxic effects of tributyltin (TBT) and inorganic lead (PbII) in rats.
abstract: This study evaluated the toxic effects of two doses of inorganic lead (PbII) and tributyltin (TBT), separately and together in different exposure times (30 and 60 days) in rats. After exposure, liver, kidney, brain and blood were sampled for histopathological, hematological and enzymatic analysis. The number of peritoneal cells and lipopolysaccharide (LPS)-induced neutrophil migration after exposure was also analyzed. The liver presented necrotic areas in all exposed individuals while hematological and enzymatic parameters showed no changes. TBT, but not PbII, reduced the number of resident peritoneal macrophages. The combination of both toxicants abolished TBT effects at lower doses and even increased the number of macrophages at higher doses. The neutrophil migration was increased by lead and lead associated with TBT. These results confirm the potential hepatotoxicity of these compounds and they may have antagonistic effects on the immune cells when administered alone. The combination of toxicants induced an increased inflammatory response suggesting that lead effects may prevail over TBT reduction on macrophage number.
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, Caixa Postal 19031, CEP 81, 531-990 Curitiba, Brazil.
authors.forename: Helena Cristina
authors.initials: HC
authors.lastname: da Silva de Assis
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sánchez-Chardi
authors.forename: Renata Cristiane
authors.initials: RC
authors.lastname: Dos Reis
authors.forename: Lilian
authors.initials: L
authors.lastname: Nicaretta
authors.forename: Cinthia
authors.initials: C
authors.lastname: Mencinauski
authors.forename: Sibelle Christine Glaser
authors.initials: SC
authors.lastname: Jakobi
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: da Silva
authors.forename: Aleksander Roberto
authors.initials: AR
authors.lastname: Zampronio
authors.forename: Emillien
authors.initials: E
authors.lastname: Pelletier
authors.forename: Ciro Alberto
authors.initials: CA
authors.lastname: de Oliveira Ribeiro