title: [Good laboratory practice of equilibrium solubility measurement II. Study of pH-dependent solubility of ionizable compounds].
abstract: In this paper the pH-equilibrium solubility profiles of ionizable drugs are presented. The aim of the present work was to study the validity of the Henderson-Hasselbalch (HH) relationship in the case of structurally diverse weak bases. In the case of monoprotic bases, namely papaverine, promethazine and propafenone the experimental equilibrium solubility data precisely follow the theoretical HH curve until the limit of salt solubility. The common ion effect on salt solubility was found to be significant at low pHs. Deviation from the HH equation in the case of dibasic quetiapine hydrogen fumarate can be easily interpreted with the formation of different salt compositions. The significance of pH control and the effect of the salt form (e.g., fumarate) was also investigated. It is critical that the pKa value and the intrinsic solubility are accurately determined when the HH relationship is used to predict the pH-dependent aqueous solubility of drugs.
authors.affiliation: Semmelweis Egyetem, Gyógyszerészi Kémiai Intézet, 1092 Budapest, Hogyes Endre u. 9.
authors.forename: Gergely
authors.initials: G
authors.lastname: Völgyi
authors.forename: Edit
authors.initials: E
authors.lastname: Baka
authors.forename: Márta
authors.initials: M
authors.lastname: Kovács
authors.forename: Novák Krisztina
authors.initials: NK
authors.lastname: Takácsné
mesh_terms: D003987:Dibenzothiazepines / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007753:Laboratories / Q000592:standards; D010208:Papaverine / Q000737:chemistry; D011398:Promethazine / Q000737:chemistry; D011405:Propafenone / Q000737:chemistry; D000069348:Quetiapine Fumarate; D015203:Reproducibility of Results; D012965:Sodium Chloride / Q000737:chemistry; D012995:Solubility; D014867:Water
chemical_list: D003987:Dibenzothiazepines; D007477:Ions; D014867:Water; D000069348:Quetiapine Fumarate; D012965:Sodium Chloride; D011405:Propafenone; D010208:Papaverine; D011398:Promethazine