title: OXA-198, an acquired carbapenem-hydrolyzing class D beta-lactamase from Pseudomonas aeruginosa.
abstract: A carbapenem-resistant Pseudomonas aeruginosa strain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression in Escherichia coli TOP10 and in P. aeruginosa PAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. The bla(OXA-198) gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance in P. aeruginosa.
authors.affiliation: Laboratoire de Bactériologie, CHU Mont-Godinne, 1 Av. Dr. Gaston Thérasse, B-5530 Yvoir, Belgium. farid.elgarch@uclouvain.be
authors.forename: Farid
authors.initials: F
authors.lastname: El Garch
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.forename: Carine
authors.initials: C
authors.lastname: Bebrone
authors.forename: Moreno
authors.initials: M
authors.lastname: Galleni
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015780:Carbapenems / Q000378:metabolism* / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D032023:Integrons / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification / Q000378:metabolism; D020539:Sequence Analysis, Protein; D013982:Ticarcillin / Q000494:pharmacology; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase; D013982:Ticarcillin