title: Activation of NK cells by ADCC antibodies and HIV disease progression.
abstract: Antibody-dependent cellular cytotoxicity (ADCC) is of considerable interest as an immune response that may facilitate the control of HIV infection. We studied ADCC responses prospectively in a cohort of 79 HIV-positive subjects followed up for a mean of 2.3 years without antiretroviral therapy. We used a novel assay of the ability of ADCC to activate natural killer (NK) cells, either from the same HIV-positive subject or from a healthy blood donor. We found that ADCC responses to either gp140 Env protein or HIV peptide pools were common in HIV-positive subjects when NK cells from the HIV-positive subject were used but did not correlate with markers of HIV disease progression. In contrast, ADCC responses to whole gp140 Env protein were strongly associated with a slower decline in CD4 T-cell loss when healthy donor NK cells were used as effectors. Our data had implications for induction of the most effective ADCC responses by HIV vaccines.
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Amy W
authors.initials: AW
authors.lastname: Chung
authors.forename: Marjon
authors.initials: M
authors.lastname: Navis
authors.forename: Gamze
authors.initials: G
authors.lastname: Isitman
authors.forename: Leia
authors.initials: L
authors.lastname: Wren
authors.forename: Julie
authors.initials: J
authors.lastname: Silvers
authors.forename: Janaki
authors.initials: J
authors.lastname: Amin
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Kent
authors.forename: Ivan
authors.initials: I
authors.lastname: Stratov
mesh_terms: D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D018791:CD4 Lymphocyte Count; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D054298:Human Immunodeficiency Virus Proteins / Q000097:blood / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008137:Longitudinal Studies; D008213:Lymphocyte Activation; D052118:Lysosomal-Associated Membrane Protein 1 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D019562:Viral Load / Q000276:immunology; D055815:Young Adult; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology
chemical_list: D054298:Human Immunodeficiency Virus Proteins; D052118:Lysosomal-Associated Membrane Protein 1; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1; D007371:Interferon-gamma