title: A novel method of plasmid isolation using laundry detergent.
abstract: Since the discovery of plasmid, various methods have been developed to isolate plasmid DNA. All the methods have one common and important target of isolating plasmid DNA of high quality and quantity in less time. These methods are not completely safe because of use of toxic chemicals compounds. The developed protocol for plasmid extraction is based on the alkaline lysis method of plasmid preparation (extraction atpH 8.0) with slight modifications. Cell lysis reagent sodium dodecyl sulfate is replaced by lipase enzyme present in laundry detergent. A good plasmid preparation can be made, which is well suited for subsequent molecular biology applications. By taking safety measures on count, contaminants like, RNA and protein can be completely avoided with maximized plasmid yield. The resultant plasmid quality and quantity can be well comparable to other prevalent methods.
authors.affiliation: Animal Biotechnology Centre, National Dairy Research Institute, Karnal 132 001, India.
authors.forename: P
authors.initials: P
authors.lastname: Yadav
authors.forename: A
authors.initials: A
authors.lastname: Yadav
authors.forename: V
authors.initials: V
authors.lastname: Garg
authors.forename: T K
authors.initials: TK
authors.lastname: Datta
authors.forename: S L
authors.initials: SL
authors.lastname: Goswami
authors.forename: S
authors.initials: S
authors.lastname: De
mesh_terms: D000818:Animals; D002020:Buffaloes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000302:isolation & purification*; D003902:Detergents / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics; D008049:Lipase / Q000737:chemistry; D010957:Plasmids / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013053:Spectrophotometry
chemical_list: D004269:DNA, Bacterial; D003902:Detergents; D015292:Glycoprotein Hormones, alpha Subunit; D008049:Lipase; D004262:DNA Restriction Enzymes