title: Kinesin molecular motor Eg5 functions during polypeptide synthesis.
abstract: The kinesin-related molecular motor Eg5 plays roles in cell division, promoting spindle assembly. We show that during interphase Eg5 is associated with ribosomes and is required for optimal nascent polypeptide synthesis. When Eg5 was inhibited, ribosomes no longer bound to microtubules in vitro, ribosome transit rates slowed, and polysomes accumulated in intact cells, suggesting defects in elongation or termination during polypeptide synthesis. These results demonstrate that the molecular motor Eg5 associates with ribosomes and enhances the efficiency of translation.
authors.affiliation: Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
authors.forename: Kristen M
authors.initials: KM
authors.lastname: Bartoli
authors.forename: Jelena
authors.initials: J
authors.lastname: Jakovljevic
authors.forename: John L
authors.initials: JL
authors.lastname: Woolford
authors.forename: William S
authors.initials: WS
authors.lastname: Saunders
mesh_terms: D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D055785:Gene Knockdown Techniques; D006801:Humans; D047468:Immunoprecipitation; D007399:Interphase; D016547:Kinesins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D011485:Protein Binding; D014176:Protein Biosynthesis; D011743:Pyrimidines / Q000494:pharmacology; D034622:RNA Interference; D012270:Ribosomes / Q000378:metabolism; D013871:Thiones / Q000494:pharmacology
chemical_list: C470243:KIF11 protein, human; D011743:Pyrimidines; D013871:Thiones; C400223:monastrol; D016547:Kinesins