title: Sodium tanshinone iia sulfonate attenuates seawater aspiration-induced acute pulmonary edema by up-regulating Na(+),K(+)-ATPase activity.
abstract: Relieving pulmonary edema is the key of a successful treatment to seawater drowning. Sodium tanshinone IIA sulfonate (STS) has been observed to reduce lung edema from lipopolysaccharide (LPS)-induced lung injury. In this study the authors investigated whether STS attenuates seawater aspiration-induced acute pulmonary edema, and examined the effects of sodium-potassium adensosine triphosphatase (Na(+),K(+)-ATPase) on it. Seawater was instilled through an endotracheal tube. The anesthetized and spontaneously breathing rats received STS intraperitoneally after seawater aspiration. Pao(2), lung wet-to-dry weight ratio, and pulmonary microvascular permeability were tested. The authors explored the effects of STS on the expression and activity of Na(+),K(+)-ATPase in vivo and in vitro. Additionally, the authors investigated the role of the extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway in the stimulation of Na(+),K(+)-ATPase by STS. The results showed that STS significantly improved hypoxemia, attenuated lung edema, and alleviated seawater-induced lung injury in vivo. Both in vivo and in vitro, it was observed that STS up-regulated the expression and activity of Na(+),K(+)-ATPase. ERK1/2 inhibitor partially blocked the effects of STS on Na(+),K(+)-ATPase activity in alveolar type II cells following seawater incubation. These results indicated that STS could improve seawater aspiration-induced acute pulmonary edema by up-regulating Na(+),K(+)-ATPase activity, and the ERK1/2 signaling pathway may be involved in it.
authors.affiliation: Department of Respiratory Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, PR China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xie
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jia-Huan
authors.initials: JH
authors.lastname: Li
authors.forename: Qi-Xin
authors.initials: QX
authors.lastname: Fan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: De-Guang
authors.initials: DG
authors.lastname: Mu
authors.forename: Wang-Ping
authors.initials: WP
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Peng-Tao
authors.initials: PT
authors.lastname: Zhao
authors.forename: Fa-Guang
authors.initials: FG
authors.lastname: Jin
authors.forename: Zhi-Chao
authors.initials: ZC
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D010616:Phenanthrenes / Q000494:pharmacology*; D011015:Pneumonia, Aspiration / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology / Q000235:genetics; D011654:Pulmonary Edema / Q000188:drug therapy* / Q000201:enzymology / Q000209:etiology* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012623:Seawater / Q000009:adverse effects*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D017931:DNA Primers; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D012333:RNA, Messenger; C024894:tanshinone II A sodium sulfonate; D000254:Sodium-Potassium-Exchanging ATPase