title: Proteomic identification of RREB1, PDE6B, and CD209 up-regulated in primitive gut tube differentiated from human embryonic stem cells.
abstract: OBJECTIVE
  This study aimed to identify proteins important for the primitive gut tube differentiation from human embryonic stem cells (hESCs) by derivation method for pancreatic cells.
  
  
  METHODS
  Proteins with altered expression levels in the process of differentiating to primitive gut tube from definitive endoderm of hESCs were investigated by comparative proteomic analysis using 2-dimensional gel electrophoresis and mass spectrometric analyses.
  
  
  RESULTS
  Differentiation to primitive gut tube from hESCs was analyzed using differentiation marker genes and proteins. Twenty-seven protein spots with significant changes in intensity were found by 2-dimensional gel electrophoresis, and 24 proteins were further identified. These proteins were functionally annotated based on gene ontology. The expression levels of 3 proteins, RREB1, PDE6B, and CD209, involved in signal transduction, were validated using quantitative reverse transcription-polymerase chain reaction and Western blot. Their mRNA and protein expression levels increased in primitive gut tube but not in definitive endoderm or embryonic body.
  
  
  CONCLUSIONS
  The increase in expression of RREB1, PDE6B, and CD209 suggests that these proteins might play important roles in the differentiation of primitive gut tube cells from hESCs and in human primitive gut tube development into pancreas. Therefore, they could be developed as differentiation markers for identifying primitive gut tube cells.
authors.affiliation: Department of Physiology, School of Medicine, CHA University, Seongnam, Gyeonggi, Republic of Korea. leedh@cha.ac.kr
authors.forename: Dong Hyeon
authors.initials: DH
authors.lastname: Lee
authors.forename: Jung Jae
authors.initials: JJ
authors.lastname: Ko
authors.forename: Young Geon
authors.initials: YG
authors.lastname: Ji
authors.forename: Hyung Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Taesun
authors.initials: T
authors.lastname: Hwang
mesh_terms: D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004064:Digestive System / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D010179:Pancreas / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D004268:DNA-Binding Proteins; D037181:Lectins, C-Type; D020543:Proteome; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C101743:RREB1 protein, human; C517431:PDE6B protein, human