title: Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells.
abstract: Using a flow cytometry-based screen of commercial antibodies, we have identified cell-surface markers for the separation of pancreatic cell types derived from human embryonic stem (hES) cells. We show enrichment of pancreatic endoderm cells using CD142 and of endocrine cells using CD200 and CD318. After transplantation into mice, enriched pancreatic endoderm cells give rise to all the pancreatic lineages, including functional insulin-producing cells, demonstrating that they are pancreatic progenitors. In contrast, implanted, enriched polyhormonal endocrine cells principally give rise to glucagon cells. These antibodies will aid investigations that use pancreatic cells generated from pluripotent stem cells to study diabetes and pancreas biology.
authors.affiliation: ViaCyte, Inc. (formerly Novocell, Inc.), San Diego, California, USA. okelly@viacyte.com
authors.forename: Olivia G
authors.initials: OG
authors.lastname: Kelly
authors.forename: Man Yin
authors.initials: MY
authors.lastname: Chan
authors.forename: Laura A
authors.initials: LA
authors.lastname: Martinson
authors.forename: Kuniko
authors.initials: K
authors.lastname: Kadoya
authors.forename: Traci M
authors.initials: TM
authors.lastname: Ostertag
authors.forename: Kelly G
authors.initials: KG
authors.lastname: Ross
authors.forename: Mike
authors.initials: M
authors.lastname: Richardson
authors.forename: Melissa K
authors.initials: MK
authors.lastname: Carpenter
authors.forename: Kevin A
authors.initials: KA
authors.lastname: D'Amour
authors.forename: Evert
authors.initials: E
authors.lastname: Kroon
authors.forename: Mark
authors.initials: M
authors.lastname: Moorman
authors.forename: Emmanuel E
authors.initials: EE
authors.lastname: Baetge
authors.forename: Anne G
authors.initials: AG
authors.lastname: Bang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D010179:Pancreas / Q000166:cytology*; D014183:Transplantation, Heterologous
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D015415:Biomarkers