title: RhoBTB2 (DBC2) functions as tumor suppressor via inhibiting proliferation, preventing colony formation and inducing apoptosis in breast cancer cells.
abstract: RhoBTB2 was isolated recently as a tumor suppressor gene from sporadic breast cancer. Although RhoBTB2 was found to be frequently lost in breast cancer and a variety of cancers, its antitumor effect, however, remains unclear. In this study, we constructed a recombinant expression vector pEGFP-N1-RhoBTB2 and transfected it into RhoBTB2-negative breast tumor cell line T-47D. Stable transformanted cells were identified by fluorescence microscope, RT-PCR and Western blot. Cell viability was measured by MTT assay. Colony forming efficiency of breast tumor cells was detected by colony formation assay. Morphological change of apoptotic cells was observed by hematoxylin-eosin staining. Apoptotic ratio was determined by flow cytometry. Cell invasion and migration ability assay were performed using transwell system. Overexpression of RhoBTB2 in breast tumor cells significantly inhibited the proliferation and colony formation of tumor cells. In addition, RhoBTB2 also elevated the apoptotic ratio and caused typical changes of apoptotic morphology in breast tumor cells of RhoBTB2 overexpression. But RhoBTB2 did not influence the invasion and migration ability of breast tumor cells. Therefore, RhoBTB2 is an important tumor suppressor gene related with breast cancer and may play antitumor roles by inhibiting proliferation, preventing colony formation and promoting the apoptosis of tumor cells. However, the precise mechanism behind the antitumor effects of RhoBTB2 needs to be investigated further.
authors.affiliation: Institute of Basic Medical Sciences, Qi Lu Hospital, Shandong University, Jinan, PR China. haitingmao@yahoo.com
authors.forename: Haiting
authors.initials: H
authors.lastname: Mao
authors.forename: Lining
authors.initials: L
authors.lastname: Zhang
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yang
authors.forename: Jintang
authors.initials: J
authors.lastname: Sun
authors.forename: Biping
authors.initials: B
authors.lastname: Deng
authors.forename: Jinbo
authors.initials: J
authors.lastname: Feng
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Shao
authors.forename: Alei
authors.initials: A
authors.lastname: Feng
authors.forename: Bingfeng
authors.initials: B
authors.lastname: Song
authors.forename: Xun
authors.initials: X
authors.lastname: Qu
mesh_terms: D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014410:Tumor Stem Cell Assay; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation
chemical_list: C497336:RHOBTB2 protein, human; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D019204:GTP-Binding Proteins