title: Preparation and evaluation of lactose-modified monoliths for the adsorption and decontamination of plant toxins and lectins.
abstract: A series of sugar-modified porous silica monoliths with different sugar ligands (β-lactoside, β-N-acetyllactosaminide, β-d-galactoside, β-d-N-acetylgalactosaminide and β-d-glucoside) and linkers were prepared and evaluated using plant toxins and lectins including ricin and a Ricinus communis agglutinin (RCA(120)). Among these sugar monoliths, a lactose monolith carrying a triethylene glycol spacer adsorbed ricin and RCA(120) with the highest efficiency. The monolith showed no binding with albumin, globulin, and lectins from Jack beans, Osage orange, Amur maackia and wheat germ. All these data support the utility of the lactose-modified monolith as a tool for adsorption and decontamination of plant toxins.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Higashi, Tsukuba 305-8565, Japan.
authors.forename: Haruhito
authors.initials: H
authors.lastname: Kato
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Uzawa
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nagatsuka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kondo
authors.forename: Keita
authors.initials: K
authors.lastname: Sato
authors.forename: Isaac
authors.initials: I
authors.lastname: Ohsawa
authors.forename: Mieko
authors.initials: M
authors.lastname: Kanamori-Kataoka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takei
authors.forename: Shigenori
authors.initials: S
authors.lastname: Ota
authors.forename: Masahiro
authors.initials: M
authors.lastname: Furuno
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Dohi
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Seto
mesh_terms: D000327:Adsorption; D003666:Decontamination / Q000379:methods*; D006027:Glycosides / Q000737:chemistry; D007785:Lactose / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry* / Q000302:isolation & purification; D012276:Ricin / Q000737:chemistry; D014118:Toxins, Biological / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D006027:Glycosides; D037121:Plant Lectins; D014118:Toxins, Biological; C008245:lactosides; D012276:Ricin; D007785:Lactose