title: Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST).
abstract: The selective isolation of a sub-population of cells from a larger, mixed population is a critical preparatory process to many biomedical assays. Here, we present a new cell isolation platform with a unique set of advantages over existing devices. Our technology, termed Immiscible Filtration Assisted by Surface Tension, exploits physical phenomena associated with the microscale to establish fluidic barriers composed of immiscible liquids. By attaching magnetically-responsive particles to a target cell population via immunocapture, we can selectively transport this population across the immiscible barrier and into a separate aqueous solution. The high interfacial energy associated with the immiscible phase / aqueous phase boundaries prevents unwanted cells or other contaminants from inadvertently crossing the immiscible phase. We have demonstrated, using fluorescent particles, stromal cells, and whole blood as "background", that we can successfully isolate ~70% of a target breast cancer cell population with an average purity of >80%. Increased purity was obtained by coupling two immiscible barriers in series, a modification that only slightly increases operational complexity. Furthermore, several samples can be processed in parallel batches in a near-instantaneous manner without the requirement of any washing, which can cause dilution (negative selection) or significant uncontrolled loss (positive selection) of target cells. Finally, cells were observed to remain viable and proliferative following traverse through the immiscible phase, indicating that this process is suitable for a variety of downstream assays, including those requiring intact living cells.
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin-Madison, 1111 Highland Ave., Madison, WI 53705, USA.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Berry
authors.forename: Lindsay N
authors.initials: LN
authors.lastname: Strotman
authors.forename: Jessica D
authors.initials: JD
authors.lastname: Kueck
authors.forename: Elaine T
authors.initials: ET
authors.lastname: Alarid
authors.forename: David J
authors.initials: DJ
authors.lastname: Beebe
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004847:Epithelial Cells; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D044085:Microfluidics / Q000379:methods*; D008962:Models, Theoretical; D013500:Surface Tension