title: Label-free optical activation of astrocyte in vivo.
abstract: As the most abundant cell type in the central nervous system, astrocyte has been one of main research topics in neuroscience. Although various tools have been developed, at present, there is no tool that allows noninvasive activation of astrocyte in vivo without genetic or pharmacological perturbation. Here we report a noninvasive label-free optical method for physiological astrocyte activation in vivo using a femtosecond pulsed laser. We showed the laser stimulation robustly induced astrocytic calcium activation in vivo and further verified physiological relevance of the calcium increase by demonstrating astrocyte mediated vasodilation in the brain. This novel optical method will facilitate noninvasive physiological study on astrocyte function.
authors.affiliation: KAIST, Department of Bio and Brain Engineering, Cell Signaling and Bioimaging Laboratory, 335 Gwahak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Myunghwan
authors.initials: M
authors.lastname: Choi
authors.forename: Jonghee
authors.initials: J
authors.lastname: Yoon
authors.forename: Taeyun
authors.initials: T
authors.lastname: Ku
authors.forename: Kyungsun
authors.initials: K
authors.lastname: Choi
authors.forename: Chulhee
authors.initials: C
authors.lastname: Choi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology* / Q000528:radiation effects*; D020013:Calcium Signaling / Q000528:radiation effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology / Q000528:radiation effects; D007834:Lasers; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D036641:Microscopy, Fluorescence, Multiphoton; D055620:Optical Phenomena; D051381:Rats; D014664:Vasodilation / Q000502:physiology / Q000528:radiation effects