title: Effects of perfluorooctane sulfonate on ion channels and glutamate-activated current in cultured rat hippocampal neurons.
abstract: Concern on an emerging persistent contaminant, perfluorooctane sulfonate (PFOS), is increasingly growing. Although the fate, transport, distribution and bioaccumulation of PFOS have been documented, its toxicological effects especially neurotoxicity remain largely unknown. In this study, the effects of PFOS on ion channels including potassium and sodium channels and exogenous glutamate-activated current in cultured rat hippocampal neurons were examined, based on whole-cell patch-clamp recording. PFOS markedly increased two subtypes of potassium currents, including transient outward current and delayed rectifier current, at doses over 10μM. PFOS did not affect the amplitude of sodium current at all administrated doses (1, 10 or 100μM) but clearly shifted the activation current-voltage curve toward negatively potential. Further, PFOS significantly altered the glutamate-activated current at all doses. Taken together these findings indicated that PFOS disturbs the neuronal physiological processes, which revealed the damage of this pollutant to nerve system and will be helpful for further exploration to its underlying mechanism.
authors.affiliation: Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, Shandong 264003, China; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Liao
authors.forename: Lin
authors.initials: L
authors.lastname: Cui
authors.forename: Qun-Fang
authors.initials: QF
authors.lastname: Zhou
authors.forename: Shu-Min
authors.initials: SM
authors.lastname: Duan
authors.forename: Gui-Bin
authors.initials: GB
authors.lastname: Jiang