title: Multi-Tox: application of the ToxR-transcriptional reporter assay to the study of multi-pass protein transmembrane domain oligomerization.
abstract: ToxR-based transcriptional reporter assays allow the strength of transmembrane helix interactions in biological membranes to be measured. Previously, these assays have only been used to study single-pass transmembrane systems. To facilitate investigation of polytopic transmembrane domain (TMD) oligomerization, we applied the ToxR methodology to the study of multi-pass TMD oligomerization to give 'Multi-Tox'. Association propensities of the viral oncoprotein, latent membrane protein-1 (LMP-1), and the E. coli membrane-integral diacylglycerol kinase (DAGK) were studied by Multi-Tox, highlighting residues of particular mechanistic importance. Both homo- and hetero-oligomerizations were studied.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309-0215, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Joce
authors.forename: Alyssa A
authors.initials: AA
authors.lastname: Wiener
authors.forename: Hang
authors.initials: H
authors.lastname: Yin
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001704:Biopolymers / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019852:Diacylglycerol Kinase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D001704:Biopolymers; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C066719:toxR protein, bacteria; D019852:Diacylglycerol Kinase