title: Luffa aegyptiaca (Gourd) Fruit Juice as a Source of Peroxidase.
abstract: Peroxidases have turned out to be potential biocatalyst for a variety of organic reactions. The research work reported in this communication was done with the objective of finding a convenient rich source of peroxidase which could be used as a biocatalyst for organic synthetic reactions. The studies made have shown that Luffa aegyptiaca (gourd) fruit juice contains peroxidase activity of the order of 180 enzyme unit/mL. The K(m) values of this peroxidase for the substrates guaiacol and hydrogen peroxide were 2.0 and 0.2 mM, respectively. The pH and temperature optima were 6.5 and 60°C, respectively. Like other peroxidases, it followed double displacement type mechanism. Sodium azide inhibited the enzyme competitively with K(i) value of 3.35 mM.
authors.affiliation: Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur 273 009, India.
authors.forename: R S S
authors.initials: RS
authors.lastname: Yadav
authors.forename: K S
authors.initials: KS
authors.lastname: Yadav
authors.forename: H S
authors.initials: HS
authors.lastname: Yadav