title: Hairy roots cultures from different Solanaceous species have varying capacities to produce E. coli B-subunit heat-labile toxin antigen.
abstract: The gene encoding enterotoxigenic Escherichia coli B-subunit heat-labile toxin (LTB) antigen was co-transformed into hairy root cultures of Nicotiana tabacum (tobacco), Solanum lycopersicum (tomato) and Petunia parodii (petunia) under the CaMV35S promoter. Tobacco and petunia roots contained ~65-70 μg LTB g(-1) tissue whilst hairy roots of tomato contained ~10 μg LTB g(-1). Antigen at ~600 ng ml(-1) was detected in growth medium of tobacco and petunia. Tobacco roots with higher LTB levels showed growth retardation of ~80% whereas petunia hairy roots with similar levels of LTB showed only ~35% growth retardation, relative to vector controls. Regeneration of plants from LTB-containing tobacco hairy roots was readily achieved and re-initiated hairy roots from greenhouse-grown plants showed similar growth and LTB production characteristics as the original hairy root cultures.
authors.affiliation: School of Biological Sciences, Monash University, Melbourne, VIC, Australia. Giorgio.deguzman@monash.edu.au
authors.forename: Giorgio
authors.initials: G
authors.lastname: De Guzman
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Walmsley
authors.forename: Diane E
authors.initials: DE
authors.lastname: Webster
authors.forename: John D
authors.initials: JD
authors.lastname: Hamill
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics*; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D019657:Solanaceae / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli