title: Mucosal immunotherapy in an Alzheimer mouse model by recombinant Sendai virus vector carrying Aβ1-43/IL-10 cDNA.
abstract: Based on the amyloid cascade hypothesis, many reports have indicated that immunotherapy is beneficial for Alzheimer's disease (AD). We developed a mucosal immunotherapy for AD by nasal administration of recombinant Sendai virus vector carrying Aβ1-43 and mouse IL-10 cDNA. Nasal but not intramuscular administration of the vaccine induced good antibody responses to Aβ. When APP transgenic mice (Tg2576) received this vaccine once nasally, the Aβ plaque burden was significantly decreased 8 weeks after without inducing inflammation in the brain. The amount of Aβ measured by ELISA was also reduced in both soluble and insoluble fractions of the brain homogenates, and notably the Aβ oligomer (12-mer) was also apparently decreased. Tg2576 mice showed significant improvement in cognitive functions examined at 3 months after vaccination. Thus, this is an alternative immunotherapy for AD, which has an advantage in non-invasive, safe and relatively long lasting features.
authors.affiliation: Division of Neurology, Department of Internal Medicine, Saga University Faculty of Medicine, 5-1-1 Nabeshima, Saga 849-8501, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Hara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Mouri
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Yonemitsu
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nabeshima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tabira
mesh_terms: D000544:Alzheimer Disease / Q000628:therapy*; D023582:Alzheimer Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology; D003071:Cognition; D018076:DNA, Complementary; D004195:Disease Models, Animal; D015316:Genetic Therapy; D005822:Genetic Vectors; D018928:Immunity, Mucosal; D007167:Immunotherapy / Q000379:methods*; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D018782:Maze Learning; D051379:Mice; D010446:Peptide Fragments / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D058225:Plaque, Amyloid; D029082:Sendai virus / Q000235:genetics; D014611:Vaccination
chemical_list: D023582:Alzheimer Vaccines; D016229:Amyloid beta-Peptides; D018076:DNA, Complementary; D010446:Peptide Fragments; D016753:Interleukin-10