title: Development and validation of an ion-pair RP-HPLC method for the determination of oligopeptide-20 in cosmeceuticals.
abstract: Oligopeptide-20 is a growth-factor mimicking peptide used in cosmeceuticals. This article describes the development and validation of an ion-pair reversed-phase liquid chromatography method that allows, after liquid-liquid extraction, the quantification of oligopeptide-20 in cosmetic creams. Chromatographic separation was achieved on a cyanopropyl Hypersil analytical column (100 mm × 2.1 mm i.d., 5 μm particle size), using a mobile phase of acetonitrile-heptafluorobutyric acid (pH=2.5, 9.0 mM) (70:30, v/v) containing 0.045% diethylamine at a flow rate of 0.50 mL min(-1). Ultraviolet (UV) spectrophotometric detection at 225 nm was used. The method had linear calibration curve over the range 1.35-4.95 μg mL(-1) for oligopeptide-20. The intra- and inter-day RSD values were less than 3.3%, while the relative percentage error, %E(r), was less than 1.9. The developed method was applied successfully to the quality control of a cosmetic cream containing 0.003% (w/w) oligopeptide-20.
authors.affiliation: University of Athens, School of Pharmacy, Division of Pharmaceutical Chemistry, Panepistimiopolis, Zografou 157 71, Athens, Greece.
authors.forename: Panagiota
authors.initials: P
authors.lastname: Papagianni
authors.forename: Athanasia
authors.initials: A
authors.lastname: Varvaresou
authors.forename: Spyros
authors.initials: S
authors.lastname: Papageorgiou
authors.forename: Irene
authors.initials: I
authors.lastname: Panderi
mesh_terms: D002138:Calibration; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D003358:Cosmetics / Q000032:analysis* / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D003358:Cosmetics; D007477:Ions; D009842:Oligopeptides