title: Efficacy of oral E1210, a new broad-spectrum antifungal with a novel mechanism of action, in murine models of candidiasis, aspergillosis, and fusariosis.
abstract: E1210 is a first-in-class, broad-spectrum antifungal with a novel mechanism of action-inhibition of fungal glycosylphosphatidylinositol biosynthesis. In this study, the efficacies of E1210 and reference antifungals were evaluated in murine models of oropharyngeal and disseminated candidiasis, pulmonary aspergillosis, and disseminated fusariosis. Oral E1210 demonstrated dose-dependent efficacy in infections caused by Candida species, Aspergillus spp., and Fusarium solani. In the treatment of oropharyngeal candidiasis, E1210 and fluconazole each caused a significantly greater reduction in the number of oral CFU than the control treatment (P < 0.05). In the disseminated candidiasis model, mice treated with E1210, fluconazole, caspofungin, or liposomal amphotericin B showed significantly higher survival rates than the control mice (P < 0.05). E1210 was also highly effective in treating disseminated candidiasis caused by azole-resistant Candida albicans or Candida tropicalis. A 24-h delay in treatment onset minimally affected the efficacy outcome of E1210 in the treatment of disseminated candidiasis. In the Aspergillus flavus pulmonary aspergillosis model, mice treated with E1210, voriconazole, or caspofungin showed significantly higher survival rates than the control mice (P < 0.05). E1210 was also effective in the treatment of Aspergillus fumigatus pulmonary aspergillosis. In contrast to many antifungals, E1210 was also effective against disseminated fusariosis caused by F. solani. In conclusion, E1210 demonstrated consistent efficacy in murine models of oropharyngeal and disseminated candidiasis, pulmonary aspergillosis, and disseminated fusariosis. These data suggest that further studies to determine E1210's potential for the treatment of disseminated fungal infections are indicated.
authors.affiliation: Next Generation Systems CFU, Eisai Product Creation Systems, Eisai Co., Ltd., 1-3 Tokodai 5-chome, Tsukuba, Ibaraki 300-2635, Japan. k-hata@hhc.eisai.co.jp
authors.forename: Katsura
authors.initials: K
authors.lastname: Hata
authors.forename: Takaaki
authors.initials: T
authors.lastname: Horii
authors.forename: Mamiko
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Nao-Aki
authors.initials: NA
authors.lastname: Watanabe
authors.forename: Miyuki
authors.initials: M
authors.lastname: Okubo
authors.forename: Jiro
authors.initials: J
authors.lastname: Sonoda
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Nakamoto
authors.forename: Keigo
authors.initials: K
authors.lastname: Tanaka
authors.forename: Syuji
authors.initials: S
authors.lastname: Shirotori
authors.forename: Norio
authors.initials: N
authors.lastname: Murai
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
authors.forename: Masayuki
authors.initials: M
authors.lastname: Matsukura
authors.forename: Shinya
authors.initials: S
authors.lastname: Abe
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Makoto
authors.initials: M
authors.lastname: Asada
mesh_terms: D000631:Aminopyridines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001228:Aspergillosis / Q000188:drug therapy* / Q000382:microbiology; D001231:Aspergillus flavus / Q000187:drug effects; D001232:Aspergillus fumigatus / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D041022:Candida tropicalis / Q000187:drug effects; D002177:Candidiasis / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D060585:Fusariosis / Q000188:drug therapy* / Q000382:microbiology; D005670:Fusarium / Q000187:drug effects; D007555:Isoxazoles / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests
chemical_list: C570438:APX001A; D000631:Aminopyridines; D000935:Antifungal Agents; D007555:Isoxazoles