title: Aeromonas spp.-mediated cell-contact cytotoxicity is associated with the presence of type III secretion system.
abstract: In the study we examined the production of cytotonic and cytotoxic toxins and the presence of a type III secretion system (TTSS) in 64 Aeromonas spp. strains isolated from fecal specimens of patients with gastroenteritis. We observed that contact of the bacteria with host epithelial cells is a prerequisite for their cytotoxicity at 3 h incubation. Cell-contact cytotoxic activity of the strains was strongly associated with the presence of the TTSS. Culture supernatants of the strains induced low cytotoxicity effects at the same time of incubation. Cell-free supernatants of 61 (95%) isolates expressed cytotoxic activity which caused the destruction of HEp-2 cells at 24 h. Moreover, 44% strains were cytotonic towards CHO cells and 46% of strains invaded epithelial cells.
authors.affiliation: Department of Microbiology, Faculty of Biology A. Mickiewicz University, 61-614 Poznań, ul. Umultowska 89, Poznan, Poland. sylkrzym@amu.edu.pl
authors.forename: Sylwia
authors.initials: S
authors.lastname: Krzymińska
authors.forename: Joanna
authors.initials: J
authors.lastname: Mokracka
authors.forename: Ryszard
authors.initials: R
authors.lastname: Koczura
authors.forename: Anna
authors.initials: A
authors.lastname: Cwiertnia
authors.forename: Adam
authors.initials: A
authors.lastname: Kaznowski
mesh_terms: D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D016466:CHO Cells; D006224:Cricetinae; D003603:Cytotoxins / Q000235:genetics / Q000378:metabolism*; D005759:Gastroenteritis / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D056945:Hep G2 Cells; D006801:Humans; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D003603:Cytotoxins