title: Fluid shear stress alters the hemostatic properties of endothelial outgrowth cells.
abstract: Surface endothelialization is an attractive means to improve the performance of small diameter vascular grafts. While endothelial outgrowth cells (EOCs) are considered a promising source of autologous endothelium, the ability of EOCs to modulate coagulation-related blood activities is not well understood. The goal of this study was to assess the role of arterial flow conditions on the thrombogenic phenotype of EOCs. EOCs derived from baboon peripheral blood, as well as mature arterial endothelial cells from baboons, were seeded onto adsorbed collagen, then exposed to physiologic levels of fluid shear stress. For important hemostatic pathways, cellular responses to shear stress were characterized at the gene and protein level and confirmed with a functional assay for activated protein C (APC) activity. For EOCs, fluid shear stress upregulated gene and protein expression of anticoagulant and platelet inhibitory factors, including thrombomodulin, tissue factor pathway inhibitor, and nitric oxide synthase 3 (eNOS). Fluid shear stress significantly altered the functional activity of EOCs by increasing APC levels. This study demonstrates that fluid shear stress is an important determinant of EOC hemostatic properties. Accordingly, manipulation of EOC phenotype by mechanical forces may be important for the development of thrombo-resistant surfaces on engineered vascular implants.
authors.affiliation: Parker H. Petit Institute for Bioengineering and Bioscience and Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
authors.forename: Ann E
authors.initials: AE
authors.lastname: Ensley
authors.forename: Robert M
authors.initials: RM
authors.lastname: Nerem
authors.forename: Deirdre E J
authors.initials: DE
authors.lastname: Anderson
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Hanson
authors.forename: Monica T
authors.initials: MT
authors.lastname: Hinds
mesh_terms: D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006487:Hemostasis / Q000187:drug effects; D008297:Male; D010215:Papio; D010590:Phalloidine / Q000378:metabolism; D011486:Protein C / Q000378:metabolism; D012212:Rheology / Q000187:drug effects; D012235:Rhodamines / Q000378:metabolism; D013314:Stress, Mechanical; D013927:Thrombosis / Q000378:metabolism
chemical_list: D005353:Fibronectins; D011486:Protein C; D012235:Rhodamines; D010590:Phalloidine