title: Simultaneous analysis of six cardiovascular drugs by capillary electrophoresis coupled with electrochemical and electrochemiluminescence detection, using a chemometrical optimization approach.
abstract: CE coupled with dual electrochemical (EC) and electrochemiluminescence (ECL) detection was optimized for simultaneous analysis of six cardiovascular drugs (alprenolol, propafenone, acebutolol, verapamil, atenolol and metoprolol) via central composite design. Following this study, three critical electrophoretic factors governing the CE separation were investigated: Tris-H(3)PO(4) buffer concentration, buffer pH value and separation voltage. A modified chromatographic response was adopted for evaluating CE separation quality. Optimum conditions were achieved using Tris-H(3)PO(4) buffer 35.6 mM (pH 2.3) separated at 13.9 kV, which was employed experimentally and led to the successful simultaneous separation of the above six drugs. The good agreement of the chromatographic response was observed between predicted data and actual experimental results using these optimized conditions (RSD=3.75%). The proposed method was validated for linearity, repeatability and sensitivity, and subsequently successfully applied to determine six basic drugs in urine samples.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.forename: Derong
authors.initials: D
authors.lastname: Zhu
authors.forename: Tianyan
authors.initials: T
authors.lastname: You
mesh_terms: D000704:Analysis of Variance; D002317:Cardiovascular Agents / Q000737:chemistry / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D008956:Models, Chemical; D011405:Propafenone / Q000737:chemistry / Q000652:urine; D011412:Propanolamines / Q000737:chemistry / Q000652:urine; D012044:Regression Analysis; D015203:Reproducibility of Results; D012107:Research Design; D012680:Sensitivity and Specificity; D014700:Verapamil / Q000737:chemistry / Q000652:urine
chemical_list: D002317:Cardiovascular Agents; D011412:Propanolamines; D011405:Propafenone; D014700:Verapamil