title: Sec14l3 is specifically expressed in mouse airway ciliated cells.
abstract: Airway epithelium is a key component for airway integrity. Previously, we found that expression of the Sec14l3 gene that encodes a 45-kDa secretory protein is inversely associated with the progression of experimentally induced airway inflammation and degeneration/necrosis of alveolar epithelium. In this report, using in situ hybridization we demonstrated that the ciliated cells in mouse lung selectively express Sec14l3 mRNA. In a three-dimensional culture of mouse tracheal epithelial cells, levels of the Sec14l3 mRNA correlated with the differentiation of ciliated cells. Intranasal infection of adult mice with influenza virus resulted in a 20-fold, progressive decrease in Sec14l3 mRNA expression over 10 days post infection. These results enhance the potential value of Sec14l3 as a ciliated epithelial cell-specific biomarker for the progression of airway inflammations such as airway viral infection and asthma.
authors.affiliation: GlaxoSmithKline K.K., Tokyo, Japan.
authors.forename: Lihua
authors.initials: L
authors.lastname: Shan
authors.forename: Shinobu
authors.initials: S
authors.lastname: Noritake
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Fujiwara
authors.forename: Satoshi
authors.initials: S
authors.lastname: Asano
authors.forename: Chikako
authors.initials: C
authors.lastname: Yoshida-Noro
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Noro
authors.forename: Keizo
authors.initials: K
authors.lastname: Yamashita
authors.forename: Takao
authors.initials: T
authors.lastname: Kawakami
mesh_terms: D000818:Animals; D015415:Biomarkers; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism* / Q000821:virology; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism*; D014132:Trachea / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D012333:RNA, Messenger; C574573:Sec14l3 protein, mouse