title: Cilengitide induces autophagy-mediated cell death in glioma cells.
abstract: We studied the effect of the integrin inhibitor cilengitide in glioma cells. Cilengitide induced cell detachment and decreased cell viability, and induction of autophagy followed by cell apoptosis. In addition, cilengitide decreased the cell renewal of glioma stem-like cells (GSCs). Inhibition of autophagy decreased the cytotoxic effect of cilengitide. Pretreatment of glioma cells and GSCs with cilengitide prior to γ-irradiation resulted in a larger increase in autophagy and a more significant decrease in cell survival. We found that cilengitide induced autophagy collectively in glioma cells, xenografts, and GSCs, which contributed to its cytotoxic effects and sensitized these cells to γ-radiation.
authors.affiliation: William and Karen Davidson Laboratory of Cell Signaling and Tumorigenesis, Detroit, MI 48202, USA.
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Lomonaco
authors.forename: Susan
authors.initials: S
authors.lastname: Finniss
authors.forename: Cunli
authors.initials: C
authors.lastname: Xiang
authors.forename: Hae Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Nancy
authors.initials: N
authors.lastname: Lemke
authors.forename: Sandra A
authors.initials: SA
authors.lastname: Rempel
authors.forename: Tom
authors.initials: T
authors.lastname: Mikkelsen
authors.forename: Chaya
authors.initials: C
authors.lastname: Brodie
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects* / Q000528:radiation effects; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D005720:Gamma Rays; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D006801:Humans; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000528:radiation effects; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*; D051381:Rats; D011923:Rats, Nude; D012910:Snake Venoms / Q000627:therapeutic use*; D014183:Transplantation, Heterologous
chemical_list: D011838:Radiation-Sensitizing Agents; D012910:Snake Venoms; C422910:Cilengitide