title: Evidence that erythrocyte DARC-positive phenotype can affect the GVHD occurrence after HLA-identical sibling HSCT.
abstract: Chemokine receptors are very important players in the pathogenesis of GVHD. The aim of this study is to test the hypothesis that the lack of expression of the DARC receptor on erythrocytes can affect the GVHD incidence. A total of 105 recipients and their 105 respective sibling donors of HSCs were enrolled in this study. All patients were evaluated for acute and chronic GVHD. The DARC genotyping assay was performed using the SSP-PCR method. The case-control analyses showed that the donor DARC 146G allele and T(-46)G(146) haplotype, coding for the FY2 version of DARC, are very significant in the GVHD paradigm because they are associated with the incidence of acute effects of this outcome in recipients (p=0.007, χ²=7.200). It seems that this version of DARC receptor is a powerful facilitator of chemokine transcytosis and subsequently leukocyte migration into GVHD target organs.
authors.affiliation: The "Immunogenetic Applied to Cells Therapy" Research Unit, The Immunohaematology and HLA-Typing Department, National Blood Transfusion Centre of Tunis, 1006 Tunis, Tunisia. sellamimh@laposte.net
authors.forename: Mohamed Hichem
authors.initials: MH
authors.lastname: Sellami
authors.forename: Manel
authors.initials: M
authors.lastname: Chaabane
authors.forename: Houda
authors.initials: H
authors.lastname: Kaabi
authors.forename: Lamia
authors.initials: L
authors.lastname: Torjemane
authors.forename: Saloua
authors.initials: S
authors.lastname: Ladeb
authors.forename: Tarek Ben
authors.initials: TB
authors.lastname: Othmane
authors.forename: Slama
authors.initials: S
authors.lastname: Hmida
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004375:Duffy Blood-Group System / Q000235:genetics / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006086:Graft vs Host Disease / Q000235:genetics* / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D035781:Siblings; D014184:Transplantation, Homologous; D014416:Tunisia
chemical_list: C473745:ACKR1 protein, human; D004375:Duffy Blood-Group System; D006680:HLA Antigens; D011956:Receptors, Cell Surface