title: Growth-promoting effect of environmental endocrine disruptors on human neuroblastoma SK-N-SH cells.
abstract: Bisphenol A (BPA), a monomer component of polycarbonate plastics and epoxy resins, is widely used in many consumer products. Zearalenone (ZEA), a non-steroidal estrogenic mycotoxin, is present in high concentrations in dairy products and cereals. Numerous researches describe a possible correlation between environmental endocrine disruptors and human tumors, but only a few papers concerned solid tumors in childhood. We investigated the effects of BPA and ZEA on the proliferation in the human neuroblastoma SK-N-SH cell line. Cell counting kit-8 and flow cytometric analysis were used to determine whether BPA and ZEA promote cell proliferation. The results indicated that BPA and ZEA-mediated increase in cell proliferation is significant (p<0.05). To explore the possible underlying mechanism, additive effect of the estrogen receptor antagonist ICI182780 or insulin-like growth factor I (IGF-I) was observed. ICI182780 could inhibit these proliferative effects of BPA and ZEA. However, no synergistic or additive growth-promoting effect was noted when IGF-1 was added. These results suggested that BPA and ZEA can promote the proliferation of SK-N-SH cells, and the estrogen receptor pathway may be involved in this effect.
authors.affiliation: Department of Surgery, Children's Hospital, Shanghai Medical College, Fudan University, 183 Feng Lin Road, Shanghai 200032, China.
authors.forename: Jicui
authors.initials: J
authors.lastname: Zheng
authors.forename: Xianmin
authors.initials: X
authors.lastname: Xiao
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Zheng
authors.forename: Qiyou
authors.initials: Q
authors.lastname: Yin
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu