title: Kalanchoe pinnata inhibits mast cell activation and prevents allergic airway disease.
abstract: Aqueous extract of Kalanchoe pinnata (Kp) have been found effective in models to reduce acute anaphylactic reactions. In the present study, we investigate the effect of Kp and the flavonoid quercetin (QE) and quercitrin (QI) on mast cell activation in vitro and in a model of allergic airway disease in vivo. Treatment with Kp and QE in vitro inhibited degranulation and cytokine production of bone marrow-derived mast cells following IgE/FcɛRI crosslinking, whereas treatment with QI had no effect. Similarly, in vivo treatment with Kp and QE decreased development of airway hyperresponsiveness, airway inflammation, goblet cell metaplasia and production of IL-5, IL-13 and TNF. In contrast, treatment with QI had no effect on these parameters. These findings demonstrate that treatment with Kp or QE is effective in treatment of allergic airway disease, providing new insights to the immunomodulatory functions of this plant.
authors.affiliation: III. Medical Clinic, Department of Pulmonary Medicine, University of Mainz, Maniz, Germany.
authors.forename: E A
authors.initials: EA
authors.lastname: Cruz
authors.forename: S
authors.initials: S
authors.lastname: Reuter
authors.forename: H
authors.initials: H
authors.lastname: Martin
authors.forename: N
authors.initials: N
authors.lastname: Dehzad
authors.forename: M F
authors.initials: MF
authors.lastname: Muzitano
authors.forename: S S
authors.initials: SS
authors.lastname: Costa
authors.forename: B
authors.initials: B
authors.lastname: Rossi-Bergmann
authors.forename: R
authors.initials: R
authors.lastname: Buhl
authors.forename: M
authors.initials: M
authors.lastname: Stassen
authors.forename: C
authors.initials: C
authors.lastname: Taube
mesh_terms: D000818:Animals; D015549:Basophil Degranulation Test; D016535:Bronchial Hyperreactivity / Q000188:drug therapy* / Q000276:immunology / Q000517:prevention & control; D020397:Goblet Cells / Q000187:drug effects / Q000276:immunology; D018793:Interleukin-13 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D031962:Kalanchoe / Q000737:chemistry* / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology; D008679:Metaplasia / Q000188:drug therapy / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D018793:Interleukin-13; D015848:Interleukin-5; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C012526:quercitrin; D010047:Ovalbumin; D011794:Quercetin