title: Cationized bovine serum albumin with pendant RGD groups forms efficient biocoatings for cell adhesion.
abstract: Cationized bovine serum albumin (cBSA-147) has been modified by attaching the cyclic pentapeptide cRGDfK to its surface through linkers of different length. Coatings of these bioconjugates on glass surfaces were studied for their ability to stimulate cell adhesion. These chemically modified albumins combine a high number of positive charges which facilitate the initial cell adhesion to the surface with multiple Arg-Gly-Asp groups which enable focal adhesion of fibroblast cells by specific interactions with cell-surface receptors. The biocoatings are easily prepared within a few minutes by simple incubation from a dilute solution of the modified albumin. This constitutes a convenient approach for preparing surfaces for cell adhesion. Excellent focal adhesion of NIH 3T3 fibroblast cells on the biocoatings was observed. About 75% of the seeded cells attached to the cRGDfK-cBSA-147 coated surfaces, and 97% of them underwent focal adhesion. Adhering cells were able to grow and proliferate on the coated surfaces, confirming the outstanding biocompatibility of these biocoatings.
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Jeck Fei
authors.initials: JF
authors.lastname: Ng
authors.forename: Tanja
authors.initials: T
authors.lastname: Weil
authors.forename: Stephan
authors.initials: S
authors.lastname: Jaenicke
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials; D002412:Cations; D002417:Cattle; D002448:Cell Adhesion; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology; D005898:Glass; D017879:Isothiocyanates / Q000737:chemistry; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods; D008956:Models, Chemical; D041681:NIH 3T3 Cells; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D020099:Coated Materials, Biocompatible; D017879:Isothiocyanates; D009842:Oligopeptides; D010455:Peptides; D012710:Serum Albumin, Bovine; C037152:isothiocyanic acid; C047981:arginyl-glycyl-aspartic acid