title: Three-dimensional arrangement of short DNA oligonucleotides at surfaces via the synthesis of DNA-branched polyacrylamide brushes by SI-ATRP.
abstract: Short DNA oligonucleotide branches are incorporated into acrylamide brushes via surface initiated atom transfer radical polymerization in an attempt to increase DNA surface density by building three-dimensional molecular architectures. ATR-FTIR as well as hybridization studies followed by SPR confirm the incorporation of the DNA sequences into the polymer backbone. MALDI-TOF analysis further suggests that six acrylamide monomer units are typically separating DNA branches present on a single brushes approximately 26 units long. This new approach offers a promising alternative to SAM-based nucleic acid and aptamer sensors and could enable the realization of more complex soft materials of controlled architecture capable of both recognition and signaling by including additional optically or electrochemically active moieties.
authors.affiliation: Nanobiotechnology and Bioanalysis Group, Departament d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain. olivier.henry@urv.cat
authors.forename: Olivier Y F
authors.initials: OY
authors.lastname: Henry
authors.forename: Ahmed D
authors.initials: AD
authors.lastname: Mehdi
authors.forename: Sinead
authors.initials: S
authors.lastname: Kirwan
authors.forename: Josep Luis Acero
authors.initials: JL
authors.lastname: Sanchez
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D019313:BRCA1 Protein / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics / Q000628:therapy*; D004247:DNA / Q000737:chemistry*; D005091:Exons; D005260:Female; D015316:Genetic Therapy / Q000295:instrumentation*; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry*; D058105:Polymerization; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D019313:BRCA1 Protein; D004247:DNA; D009841:Oligonucleotides; D000099166:Polyacrylamides