title: [GM1-dot-EIA for the detection of toxin-producing Vibrio cholerae strains].
abstract: A new variant of enzyme immunoassay (EIA) has been developed on the basis of GM1 gangliosides to detect the toxin-producing Vibrio cholerae strains--GM1-dot-EIA. Experiments were run using a nitrocellulose membrane to bind GM1 gangliosides and polyclonal antitoxic serum to detect cholerogen. GM1-dot-EIA testing identified cholera toxin in 11 of 13 supernatants of V. cholerae eltor ctx(+) strains isolated from man and in 3 of 7 supernatants of V. cholerae eltor ctx(+) strains isolated from water. These data agree with those obtained in CM1-EIA. There was no reaction with the supernatants of other microorganisms. The sensitivity of the technique was 10 ng/ml. Thus, the simple and specific GM1-dot-EIA may be recommended to detect toxin-producing V cholerae strains isolated from man and water.
authors.forename: O V
authors.initials: OV
authors.lastname: Markina
authors.forename: L P
authors.initials: LP
authors.lastname: Alekseeva
authors.forename: N R
authors.initials: NR
authors.lastname: Telesmanich
authors.forename: O S
authors.initials: OS
authors.lastname: Chemisova
authors.forename: M V
authors.initials: MV
authors.lastname: Akulova
authors.forename: N V
authors.initials: NV
authors.lastname: Markin
mesh_terms: D002771:Cholera / Q000175:diagnosis*; D002772:Cholera Toxin / Q000032:analysis*; D003101:Collodion; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000639:trends; D005677:G(M1) Ganglioside; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014734:Vibrio cholerae / Q000302:isolation & purification / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D005677:G(M1) Ganglioside; D003101:Collodion; D002772:Cholera Toxin