title: P-glycoprotein induction by breast milk attenuates intestinal inflammation in experimental necrotizing enterocolitis.
abstract: P-glycoprotein (Pgp), a product of the multi-drug resistance gene MDR1a, is a broad specificity efflux ATP cassette transmembrane transporter that is predominantly expressed in epithelial tissues. Because mdr1a(-/-) mice tend to develop spontaneous colitis in bacteria-dependent manner, Pgp is believed to have a role in protection of the intestinal epithelium from luminal bacteria. Here we demonstrate that levels of Pgp in the small intestine of newborn rodents dramatically increase during breastfeeding, but not during formula feeding (FF). In rats and mice, levels of intestinal Pgp peak on days 3-7 and 1-5 of breastfeeding, respectively. The mdr1a(-/-) neonatal mice subjected to FF, hypoxia, and hypothermia have significantly higher incidence and pathology, as well as significantly earlier onset of necrotizing enterocolitis (NEC) than congenic wild type mice. Breast-fed mdr1a(-/-) neonatal mice are also more susceptible to intestinal damage caused by the opportunistic pathogen Cronobacter sakazakii that has been associated with hospital outbreaks of NEC. Breast milk, but not formula, induces Pgp expression in enterocyte cell lines in a dose- and time-dependent manner. High levels of ectopically expressed Pgp protect epithelial cells in vitro from apoptosis induced by C. sakazakii. Taken together, these results show that breast milk-induced expression of Pgp may have a role in the protection of the neonatal intestinal epithelium from injury associated with nascent bacterial colonization.
authors.affiliation: Division of Pediatric Surgery, Saban Research Institute, Childrens Hospital Los Angeles, Los Angeles, CA 90027, USA.
authors.forename: Yigit S
authors.initials: YS
authors.lastname: Guner
authors.forename: Ashanti L
authors.initials: AL
authors.lastname: Franklin
authors.forename: Nikunj K
authors.initials: NK
authors.lastname: Chokshi
authors.forename: Shannon L
authors.initials: SL
authors.lastname: Castle
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Pontarelli
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Larry
authors.initials: L
authors.lastname: Wang
authors.forename: Nemani V
authors.initials: NV
authors.lastname: Prasadarao
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Upperman
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Grishin
authors.forename: Henri R
authors.initials: HR
authors.lastname: Ford
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D044083:Cronobacter sakazakii; D017931:DNA Primers / Q000235:genetics; D004756:Enterobacteriaceae Infections / Q000378:metabolism; D020345:Enterocolitis, Necrotizing / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000382:microbiology*; D051379:Mice; D018345:Mice, Knockout; D008892:Milk / Q000378:metabolism*; D041941:Milk Substitutes / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D014162:Transfection
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D017931:DNA Primers; C095122:multidrug resistance protein 3