title: TiO2-ZrO2 affinity chromatography polymeric microchip for phosphopeptide enrichment and separation.
abstract: We fabricated a TiO(2)-ZrO(2) affinity chromatography micro-column on 2 mm PMMA plates, and demonstrated the enrichment and separation of (a) a standard mono- and tetra-phosphopeptide, and (b) phosphopeptides contained in a tryptic digest of β-Casein. The chromatography column consisted of 32 parallel microchannels with common input and output ports and was fabricated by lithography directly on the polymeric substrate followed by plasma etching (i.e. standard MEMS processing) and sealed with lamination. The liquid deposited TiO(2)-ZrO(2) stationary phase was characterized by X-ray diffraction and was found to be mostly TiO(2) and ZrO(2) in crystalline phases. Off-chip UV detection and MALDI MS identification of the separated effluents were used. The chip had a capacity of >1.4 μg (0.7 nmol) of a prototype mono-phosphopeptide and a recovery of 94 ± 3%, and can be used with small samples (less than 0.1 μL depending on the syringe pump used). The chip design allows an expansion of its capacity by means of increasing the number of parallel microchannels at a constant sample volume. Our approach provided an alternative to off-line extraction tips (with typical capacities of 1-2 μg and sample volumes of 1-10 μL), and to on-chip efforts based on packed bed and frit formats.
authors.affiliation: Institute of Microelectronics, NCSR Demokritos, P.O. BOX 60228, 153 10, Aghia Paraskevi, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Tsougeni
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Zerefos
authors.forename: Angeliki
authors.initials: A
authors.lastname: Tserepi
authors.forename: Antonia
authors.initials: A
authors.lastname: Vlahou
authors.forename: Spiros D
authors.initials: SD
authors.lastname: Garbis
authors.forename: Evangelos
authors.initials: E
authors.lastname: Gogolides
mesh_terms: D002364:Caseins / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014025:Titanium / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry*
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides; C009495:titanium dioxide; D015040:Zirconium; D014025:Titanium; D014357:Trypsin; C028541:zirconium oxide