title: Bifunctional trehalose anode incorporating two covalently linked enzymes acting in series.
abstract: A bienzymatic electrode incorporating trehalase (Tre) and glucose oxidase (GOx) covalently bound to the surface of Pt through a functionalized thiol linker (Tre|GOx|Pt) has been designed and assembled and its catalytic properties examined by chemical and electrochemical methods in aqueous phosphate buffer solutions (PBS, pH 7.4). Exposure of Tre|GOx|Pt to PBS containing trehalose (Tr) and subsequent polarization at 0.6 V versus Ag/AgCl yielded after a few minutes well-defined steady-state currents ascribed to the oxidation of hydrogen peroxide generated by the GOx-mediated oxidation of glucose (Gl) produced by the Tre-mediated dissociation of Tr. Plots of the steady-state currents versus [Tr] over the range examined, i.e., 5-25 mM, were found to be linear. Implications of these results toward the development of an implantable biofuel cell as an autonomous energy conversion device for insects are discussed.
authors.affiliation: Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-7078, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Rasmussen
authors.forename: Richard
authors.initials: R
authors.lastname: West
authors.forename: Jim
authors.initials: J
authors.lastname: Burgess
authors.forename: Irene
authors.initials: I
authors.lastname: Lee
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherson
mesh_terms: D055162:Biocatalysis; D004566:Electrodes; D005947:Glucose / Q000096:biosynthesis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D009942:Organometallic Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D010984:Platinum / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D014198:Trehalase / Q000737:chemistry / Q000378:metabolism*; D014199:Trehalose / Q000737:chemistry* / Q000378:metabolism
chemical_list: D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds; D010984:Platinum; D014199:Trehalose; D005949:Glucose Oxidase; D014198:Trehalase; D005947:Glucose