title: High production of β-glucosidase by Aspergillus niger on corncob.
abstract: Using low-cost raw material is an effective approach for reducing the cost of cellulolytic enzymes. The farmland waste corncob was found in this study to be the best carbon source for the production of β-glucosidase by Aspergillus niger. The maximum yield of β-glucosidase activity was 48.7 IU ml(-1) by using 50 g l(-1) of corncob powder as the substrate. It was found that the water-soluble components of the corncob could increase β-glucosidase production significantly only when mixed with Avicel or wheat bran. The soluble components could not enhance the biomass and β-glucosidase production when used alone. On the other hand, the water-insoluble components of the corncob still produced high level of β-glucosidase (30 IU ml(-1)) although lower than that of using whole corncob. The results suggested that the water-insoluble components of corncob were beneficial for β-glucosidase production. It was further demonstrated that the xylan in the water-insoluble parts of corncob was the important factor in producing β-glucosidase by A. niger.
authors.affiliation: College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Wang
authors.forename: Gaihong
authors.initials: G
authors.lastname: Wu
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
mesh_terms: D001234:Aspergillus niger / Q000187:drug effects / Q000201:enzymology*; D002244:Carbon / Q000494:pharmacology; D002482:Cellulose / Q000494:pharmacology; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D012995:Solubility / Q000187:drug effects; D014990:Xylans / Q000494:pharmacology; D003313:Zea mays / Q000378:metabolism*; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D011134:Polysaccharides; D014990:Xylans; D002244:Carbon; C007916:hemicellulose; D002482:Cellulose; D001617:beta-Glucosidase; D043364:Endo-1,4-beta Xylanases