title: Effects of tributyltin and 17β-estradiol on immune and lysosomal systems of the Asian clam Corbicula fluminea (M.).
abstract: Freshwater clams Corbicula fluminea were experimentally exposed to a range of tributyltin (TBT) (50, 250 and 500ng Sn/L) and 17β-estradiol (20, 200, 2000ng/L) for 30 days. After 15 and 30 days, phagocytosis activity of haemocytes and lysosomal structural changes in the digestive cells were assayed. 17β-Estradiol exerted a higher inhibition on phagocytosis than tributyltin. This would suggest the existence of estrogen receptors, influencing the immune function. The stereological parameters measured for lysosomal structural changes in animals exposed to tributyltin varied as observed in other studies. Tributyltin is then depurated as other contaminants via digestive cell lysosomal compartment. This experiment emphasized a possible approach on the influence of endocrine disrupting compounds on a hermaphroditic species for environmental surveys.
authors.affiliation: LPTC Toxicologie Biochimique, Université de Bordeaux 1, UMR CNRS 5472, avenue des facultés, 33405 Talence cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Champeau
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Narbonne