title: Photochemical control of bacterial signal processing using a light-activated erythromycin.
abstract: Bacterial cells control resistance to the macrolide antibiotic erythromycin using the MphR(A) repressor protein. Erythromycin binds to MphR(A), causing release of the PmphR promoter, activating expression of the 2'-phosphotransferase Mph(A). We engineered the MphR(A)/promoter system to, in conjunction with a light-activatable derivative of erythromycin, enable photochemical activation of gene expression in E. coli. We applied this photochemical gene switch to the construction of a light-triggered logic gate, a light-controlled band-pass filter, as well as spatial and temporal control of gene expression.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Gardner
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.forename: Alexandria
authors.initials: A
authors.lastname: Mara
authors.forename: T Ashton
authors.initials: TA
authors.lastname: Cropp
authors.forename: Alexander
authors.initials: A
authors.lastname: Deiters
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism; D008027:Light; D010777:Photochemistry / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004917:Erythromycin; D005095:Exoribonucleases; C029364:2'-phosphodiesterase