title: Quantitative proteomic analysis reveals formation of an EscL-EscQ-EscN type III complex in enteropathogenic Escherichia coli.
abstract: We characterized Orf5 and SepQ, two type III secretion (T3S) system proteins in enteropathogenic Escherichia coli, and showed that they are essential for T3S, associated with the bacterial membrane, and interact with EscN. Our findings suggest that Orf5 and SepQ are homologs of YscL and YscQ from Yersinia, respectively.
authors.affiliation: Michael Smith Laboratories, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: Esther
authors.initials: E
authors.lastname: Biemans-Oldehinkel
authors.forename: Neta
authors.initials: N
authors.lastname: Sal-Man
authors.forename: Wanyin
authors.initials: W
authors.lastname: Deng
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Foster
authors.forename: B Brett
authors.initials: BB
authors.lastname: Finlay
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D013058:Mass Spectrometry; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*
chemical_list: D029968:Escherichia coli Proteins