title: Preventive effect of tert-butylhydroquinone on cisplatin-induced nephrotoxicity in rats.
abstract: Cis-diamminedichloroplatinum II (CDDP)-induced nephrotoxicity is associated with the overproduction of reactive oxygen species. tert-Butylhydroquinone (tBHQ) is a compound widely used as food antioxidant. The purpose of this study was to investigate the ability of tBHQ to prevent the nephrotoxic effect of CDDP in rats as well as the mechanisms involved. Thirty-six Wistar rats divided in the following groups were used: control, tBHQ (12.5mg/kg), CDDP (7.5mg/kg) and tBHQ+CDDP. Twenty-four h urine was collected at the beginning and at the end of the experiment and the rats were sacrificed 72h after CDDP-administration. Histological studies were performed and markers of renal function and oxidative/nitrosative stress were measured. In addition, the activity of the following antioxidant enzymes was measured: glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione-S-transferase (GST). CDDP-induced renal dysfunction, structural damage and oxidative/nitrosative were prevented by tBHQ. In addition, tBHQ completely prevented the CDDP-induced fall in GPx and GST activities. In conclusion, the present study indicates that the antioxidant activity of tBHQ is associated with its nephroprotective effect against CDDP-induced acute kidney injury in rats.
authors.affiliation: Facultad de Química, Departamento de Biología, Universidad Nacional Autónoma de México, Mexico City, Mexico. jazminmarlen@gmail.com
authors.forename: Jazmin M
authors.initials: JM
authors.lastname: Pérez-Rojas
authors.forename: Carlos Enrique
authors.initials: CE
authors.lastname: Guerrero-Beltrán
authors.forename: Cristino
authors.initials: C
authors.lastname: Cruz
authors.forename: Dolores J
authors.initials: DJ
authors.lastname: Sánchez-González
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Martínez-Martínez
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverri
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000097:blood; D002945:Cisplatin / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D005982:Glutathione Transferase / Q000097:blood; D006873:Hydroquinones / Q000494:pharmacology*; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000447:Aldehydes; D000975:Antioxidants; D006873:Hydroquinones; D008315:Malondialdehyde; C018855:2-tert-butylhydroquinone; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; C027576:4-hydroxy-2-nonenal; D002945:Cisplatin