title: Force measurements of TCR/pMHC recognition at T cell surface.
abstract: The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation.
authors.affiliation: Laboratoire Adhésion et Inflammation, Parc Scientifique et Technologique de Luminy, Marseille, France. pierre-henri.puech@inserm.fr
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Puech
authors.forename: Damien
authors.initials: D
authors.lastname: Nevoltris
authors.forename: Philippe
authors.initials: P
authors.lastname: Robert
authors.forename: Laurent
authors.initials: L
authors.lastname: Limozin
authors.forename: Claude
authors.initials: C
authors.lastname: Boyer
authors.forename: Pierre
authors.initials: P
authors.lastname: Bongrand
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002460:Cell Line; D002470:Cell Survival; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000378:metabolism*; D055595:Mechanical Phenomena; D051379:Mice; D018625:Microscopy, Atomic Force; D011485:Protein Binding; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*
chemical_list: D006649:Histocompatibility Antigens; D011948:Receptors, Antigen, T-Cell