title: Isolation and characterization of aphidicolin and chlamydosporol derivatives from Tolypocladium inflatum.
abstract: Six new secondary metabolites including two aphidicolin analogues, inflatins A (1) and B (2), and four chlamydosporol derivatives, inflatins C-F (3-6), have been isolated from the crude extract of Tolypocladium inflatum. The structures of 1-6 were determined mainly by NMR experiments, and 4 and 5 were further confirmed by X-ray crystallography. The absolute configurations of C-16 in 1 and C-5 in 3 were deduced via the circular dichroism data of the in situ formed [Rh₂(OCOCF₃)₄] complexes, whereas that of 4 was assigned by X-ray crystallography using Cu Kα radiation. Compounds 1 and 2 showed modest cytotoxicity against a panel of eight human tumor cell lines.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Cai
authors.forename: Xinfang
authors.initials: X
authors.lastname: Yu
authors.forename: Xingzhong
authors.initials: X
authors.lastname: Liu
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Che
mesh_terms: D016590:Aphidicolin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D011753:Pyrones; C561982:inflatin A; C561983:inflatin B; C065449:chlamydosporal; D016590:Aphidicolin