title: Efficacy of polymeric encapsulated C5a peptidase-based group B streptococcus vaccines in a murine model.
abstract: OBJECTIVE
  The purpose was to examine in mice the efficacy of various polymeric-encapsulated C5a peptidase vaccine formulations in eliciting a long-term immune response and preventing group B streptococcus (GBS) infection.
  
  
  METHODS
  C5a peptidase was encapsulated in semipermeable microspheres of poly(lactide-coglycolide) (PLGA). Female ICR mice were immunized with 0, 10, or 30 μg of encapsulated C5a peptidase within 2 different formulations of PLGA polymers. Booster doses were given at weeks 4 and 8. Antibody responses were measured by enzyme-linked immunosorbent assay at weeks 4, 8, 11, and 40. Vaginal challenges with GBS types 1a, III, and V were performed at week 12.
  
  
  RESULTS
  Thirty microgram doses of the 75:25 and 50:50 PLGA formulations generate the highest and most sustained C5a peptidase-specific immune responses. Mice that received encapsulated C5a peptidase were significantly protected from vaginal colonization compared with mice that received empty microspheres.
  
  
  CONCLUSIONS
  Encapsulated C5a peptidase elicited significant immune responses and protection against a GBS challenge. C5a peptidase microsphere encapsulation has potential as a GBS vaccine.
authors.affiliation: Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA, USA.
authors.forename: Donna A
authors.initials: DA
authors.lastname: Santillan
authors.forename: Karishma K
authors.initials: KK
authors.lastname: Rai
authors.forename: Mark K
authors.initials: MK
authors.lastname: Santillan
authors.forename: Yogita
authors.initials: Y
authors.lastname: Krishnamachari
authors.forename: Aliasger K
authors.initials: AK
authors.lastname: Salem
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Hunter
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000818:Animals; D010450:Endopeptidases / Q000276:immunology*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D008863:Microspheres; D013290:Streptococcal Infections / Q000517:prevention & control*; D022541:Streptococcal Vaccines / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D022541:Streptococcal Vaccines; D010450:Endopeptidases; C053088:C5a peptidase