title: Reassessment of the size of the supermolecular state of Dishevelled-3.
abstract: Amendment of the interpretation of recently published size-exclusion chromatography data for Dishevelled-3 on Superdex 200 and Sephacryl S-400 has led to an increase in the estimated size of the supermolecular state from 2000 to 35 000 kDa, a value that essentially duplicates the redetermined and reported estimates obtained by fluorescence correlation spectroscopy on live cells. The earlier discrepancy between the sizes of the extensively aggregated form of this scaffold protein in vivo and in vitro is thereby eliminated.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada. patelt@cc.manitoba.ca
authors.forename: Trushar R
authors.initials: TR
authors.lastname: Patel
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Winzor
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D000072261:Dishevelled Proteins; D006801:Humans; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000606584:DVL3 protein, human; D000072261:Dishevelled Proteins; D010750:Phosphoproteins