title: Blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.
abstract: A growing body of evidence suggests the potential role of chymase in organ injury in diabetes. We investigated blood glucose levels and survival in transgenic mice carrying the human chymase gene (Tg). Intraperitoneal injections of streptozotocin (STZ) (200, 100, 75 and 50 mg/kg in total, i.p.) were given to uninephrectomized Tg mice and wild-type C57BL/6 (BL) mice. Before STZ injection, the Tg mice had significantly lower body weights and slightly higher systolic blood pressure as compared with the BL mice. STZ-treated Tg mice showed significantly higher postprandial blood glucose levels as compared with the STZ-treated BL mice. The survival prevalence of STZ-treated Tg mice was zero, whereas BL mice showed a value of 40% until 42 days. STZ (100, 75 or 50 mg/kg, i.p.)-treated Tg mice also showed a similar pattern as compared with the STZ-treated BL mice. These data suggest that human chymase contributes to blood glucose levels and mortality during the progression of diabetes.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Kagawa University, Kagawa, Japan. krafiq73@yahoo.com
authors.forename: Kazi
authors.initials: K
authors.lastname: Rafiq
authors.forename: Shamshad J
authors.initials: SJ
authors.lastname: Sherajee
authors.forename: Yu-Yan
authors.initials: YY
authors.lastname: Fan
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Fujisawa
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Junji
authors.initials: J
authors.lastname: Matsuura
authors.forename: Naoki
authors.initials: N
authors.lastname: Hase
authors.forename: Hidenori
authors.initials: H
authors.lastname: Urata
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakano
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hitomi
authors.forename: Akira
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001794:Blood Pressure / Q000502:physiology; D053818:Chymases / Q000235:genetics* / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000401:mortality / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism / Q000401:mortality / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010641:Phenotype
chemical_list: D001786:Blood Glucose; D053818:Chymases