title: Application of immunocytochemistry and BRAF mutational analysis to direct smears of metastatic melanoma.
abstract: BACKGROUND
  The cytodiagnosis of melanoma in fine-needle aspiration (FNA) specimens can be challenging, often requiring the use of immunocytochemistry. As constitutively activating mutations in the BRAF oncogene are present in at least 40% of melanomas, the use of FNA material to interrogate the BRAF mutational status is likely to increase. Because cell blocks, traditionally used for these studies, can occasionally exhibit insufficient tumor cellularity, the authors investigated the utility of direct smears for immunocytochemistry and BRAF mutational analysis.
  
  
  METHODS
  Immunocytochemistry for S-100, HMB-45, and Mart-1 was prospectively performed on direct smears in 17 FNAs of metastatic melanoma. Next, BRAF sequencing was performed using DNA isolated from archived Diff-Quik-stained direct smears for 15 cases. In parallel, sequencing was performed using DNA obtained from corresponding cell blocks.
  
  
  RESULTS
  S-100 positivity in the tumor cells was observed in all 17 cases. HMB-45 and Mart-1 positivity was noted in 81% and 88% of cases, respectively. All 3 markers were positive in 76% of cases. Next, of the 15 archived melanoma FNAs tested, BRAF mutations were observed in 8 (53%); 5 and 3 melanomas harbored the V600E and V600K mutation, respectively. Corresponding cell blocks were also tested for all 15 cases, yielding concordant BRAF results in 14 (93%); 1 cell block yielded a false-negative result.
  
  
  CONCLUSIONS
  Cytologic direct smears represent a robust and valuable source of cellular material for immunocytochemistry and molecular studies, especially in instances in which inadequate cell block cellularity is anticipated or encountered.
authors.affiliation: Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
authors.forename: Kim
authors.initials: K
authors.lastname: Hookim
authors.forename: Michael H
authors.initials: MH
authors.lastname: Roh
authors.forename: Joseph
authors.initials: J
authors.lastname: Willman
authors.forename: Jeremiah
authors.initials: J
authors.lastname: Placido
authors.forename: Helmut C
authors.initials: HC
authors.lastname: Weigelin
authors.forename: Kristina L
authors.initials: KL
authors.lastname: Fields
authors.forename: Judy
authors.initials: J
authors.lastname: Pang
authors.forename: Bryan L
authors.initials: BL
authors.lastname: Betz
authors.forename: Stewart M
authors.initials: SM
authors.lastname: Knoepp
mesh_terms: D003581:Cytodiagnosis / Q000379:methods*; D004252:DNA Mutational Analysis; D006801:Humans; D007150:Immunohistochemistry; D008545:Melanoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf