title: Alleviation of atopic dermatitis-related symptoms by Perilla frutescens Britton.
abstract: To ascertain whether an aqueous fraction of Perilla frutescens Britton (PfB/af) has advantageous anti-atopic dermatitis activity, we used a 2,4-dinitrofluorobenzene (DNFB)-induced animal model of atopic dermatitis symptoms to investigate the effects of the extract. We performed an ear swelling assay by comparing thickness of the DNFB-induced ear, and measured the numbers of eosinophils as well as total immune cells. We analyzed the expression levels of matrix metalloproteinase (MMP)-9, interleukin (IL)-31 and of the T-bet transcription factor. The results revealed that PfB/af (100 µg/ml) exhibited strong anti-atopic dermatitis activity, interceding drastic reduction (35%) of the immune response, as measured by the thickness of ear epidermis swelling, and resulting in decreased eosinophil levels (73.7%) in adjacent skin tissues. Collectively, the present results suggest that PfB/af has potential for mitigation of atopic dermatitis-like symptoms induced by DNFB in the mouse.
authors.affiliation: Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Heo
authors.forename: Dong-Yoon
authors.initials: DY
authors.lastname: Nam
authors.forename: Myung Sun
authors.initials: MS
authors.lastname: Seo
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004804:Eosinophils / Q000187:drug effects; D007150:Immunohistochemistry; D007378:Interleukins / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D007378:Interleukins; D010936:Plant Extracts; C502588:interleukin-31, mouse; D004139:Dinitrofluorobenzene; D020780:Matrix Metalloproteinase 9