title: Biophysical characterization of α-amylase inhibitor Parvulustat (Z-2685) and comparison with Tendamistat (HOE-467).
abstract: Parvulustat is a small, highly active proteinaceous α-amylase inhibitor whose high-resolution NMR structure was recently solved in Frankfurt. Here, we present its biochemical and biophysical characterization. Several spectroscopic methods such as UV, fluorescence and CD were utilized to extract conformational changes upon modification of pH, temperature and chemical denaturant. Parvulustat revealed native like behavior over a wide range of denaturizing agents as reflected in terms of activity and thermodynamic data. In addition, spectroscopic and thermodynamic properties of Parvulustat were compared to the well-characterized Tendamistat. Despite the overall structural similarity, the thermodynamic stability of the two proteins is different. Our analysis led to the conclusion that Parvulustat is even more stable than Tendamistat. Furthermore, investigations on three C-terminally truncated Parvulustat derivatives indicate that the higher stability is caused by the long flexible C-terminus.
authors.affiliation: Institute of Organic Chemistry and Chemical Biology, Goethe-University, Frankfurt, Germany.
authors.forename: Alma
authors.initials: A
authors.lastname: Sokočević
authors.forename: Sigeng
authors.initials: S
authors.lastname: Han
authors.forename: Joachim W
authors.initials: JW
authors.lastname: Engels
mesh_terms: D000595:Amino Acid Sequence; D055592:Biophysical Phenomena; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011489:Protein Denaturation; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D010455:Peptides; C048177:Z 2685; D000516:alpha-Amylases; C030526:tendamistate