title: Lateral flow immunochromatographic assay for sensitive pesticide detection by using Fe3O4 nanoparticle aggregates as color reagents.
abstract: Magnetic Fe(3)O(4) particle aggregates were prepared by cross-linking Fe(3)O(4) nanoparticles bearing surface carbonyl groups with poly-L-lysine. Upon further coupling with antiparaoxon methyl polyclonal antibody, the resultant particle aggregate-based probes were used in a lateral flow immunochromatographic assay (LFIA) of pesticide residue of paraoxon methyl. The results were compared with that achieved by using the mother Fe(3)O(4) nanoparticles. More quantitative results on the signal amplification effect endowed by the controlled aggregation of Fe(3)O(4) nanoparticles were extracted by relative optical density analysis. Under optimized conditions, a detection limit of 1.7 ng/mL for paraoxon methyl was achieved by using the particle aggregates, which is almost 40-fold lower than that based on the mother Fe(3)O(4) nanoparticles.
authors.affiliation: Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing 100190, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.forename: Qiaojuan
authors.initials: Q
authors.lastname: Jia
authors.forename: Chunhui
authors.initials: C
authors.lastname: Yang
authors.forename: Ruirui
authors.initials: R
authors.lastname: Qiao
authors.forename: Lihong
authors.initials: L
authors.lastname: Jing
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Gao
mesh_terms: D000906:Antibodies / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010261:Paraoxon / Q000032:analysis / Q000276:immunology; D010575:Pesticides / Q000032:analysis* / Q000276:immunology; D011107:Polylysine / Q000737:chemistry
chemical_list: D000906:Antibodies; D004396:Coloring Agents; D058185:Magnetite Nanoparticles; D010575:Pesticides; D011107:Polylysine; D010261:Paraoxon; D052203:Ferrosoferric Oxide