title: In situ photopolymerization of biomaterials by thiol-yne click chemistry.
abstract: The thiol-yne click chemistry reaction has been used for the in situ photocrosslinking of an aliphatic hyperbranched polyester. The biocompatibility of the resulting networks has been studied and marked cytotoxicity was not found for HeLa (human cervical carcinoma) tumoral cells and COS7 fibroblasts. The photoinduced thiol-yne process allows the generation of patterned structures with different geometries in films by DLW and these materials can be used as substrates for cell adhesion. The influence of the substrate geometry on cell adhesion has been studied by culturing cells onto these substrates and a preference for the photopatterned polymeric material can be seen in some of the structures by contrast phase microscopy. Actin and vinculin fluorescent staining revealed different adhesion behavior for HeLa cells and COS7 fibroblasts and this could be assigned to the different motility of cells. The thiol-yne photoreaction has proven to be an attractive approach for the preparation of micropatterned biomaterials.
authors.affiliation: Instituto de Ciencia de Materiales de Aragón (ICMA), CSIC-Universidad de Zaragoza, Departamento de Química Orgánica, Zaragoza, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Lomba
authors.forename: Luis
authors.initials: L
authors.lastname: Oriol
authors.forename: Rafael
authors.initials: R
authors.lastname: Alcalá
authors.forename: Carlos
authors.initials: C
authors.lastname: Sánchez
authors.forename: María
authors.initials: M
authors.lastname: Moros
authors.forename: Valeria
authors.initials: V
authors.lastname: Grazú
authors.forename: José Luis
authors.initials: JL
authors.lastname: Serrano
authors.forename: Jesús M
authors.initials: JM
authors.lastname: De la Fuente
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology / Q000528:radiation effects; D019556:COS Cells; D002448:Cell Adhesion; D002465:Cell Movement; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D057930:Click Chemistry; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008422:Materials Testing; D055668:Photochemical Processes; D011091:Polyesters / Q000138:chemical synthesis* / Q000494:pharmacology / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties / Q000528:radiation effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D013438:Sulfhydryl Compounds