title: [Clinical significance of large-scale screening of A1555G mutation of mitochondria DNA for neonates].
abstract: OBJECTIVE
  To explore the necessity of large-scale screening of mitochondria DNA (mtDNA) A1555G mutation for prevention of aminoglycoside antibiotic induced deafness in newborns.
  
  
  METHODS
  One thousand blood filter samples were collected from neonates born in July 2008 in Shenzhen. DNA was extracted with Chelex-100 Resin and amplified by PCR. The mtDNA A1555G mutation was determined by denaturing high-performance liquid chromatography(DHPLC) for PCR products. The positive frequency was calculated.
  
  
  RESULTS
  The mitochondrial DNA A1555G mutation was detected in 2 cases of 1000 neonates. The frequency of mutation was 0.2%.
  
  
  CONCLUSIONS
  There is a high frequency of mtDNA A1555G mutation in neonates, the large-scale screening of mtDNAA1555G mutation in newborns might detect the individuals sensitive to aminoglycoside antibiotic, which is helpful to guide a rational medication for newborns and the maternal relatives at high-risk. Furthermore, it might be useful to prevent aminoglycoside antibiotic induced deafness.
authors.affiliation: Center for Prenatal Diagnosis, Shenzhen Maternal and Child Healthcare Hospital, Shenzhen, Guangdong 518048, P. R. China. caijun0529@hotmail.com
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.forename: Cai-qun
authors.initials: CQ
authors.lastname: Luo
authors.forename: Jian-sheng
authors.initials: JS
authors.lastname: Xie
authors.forename: Wei-qing
authors.initials: WQ
authors.lastname: Wu
authors.forename: Qian
authors.initials: Q
authors.lastname: Geng
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Hao
authors.forename: Xiao-xin
authors.initials: XX
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction
chemical_list: D004272:DNA, Mitochondrial