title: Enzyme-linked immunosorbent assay-format tissue culture infectious dose-50 test for titrating dengue virus.
abstract: A dengue nonstructural protein 1 (NS1) antigen capture enzyme-linked immunosorbent assay (ELISA)-based tissue culture infectious dose-50 (TCID(50)) test (TCID(50)-ELISA) was developed as an alternative to the standard plaque assay for titrating dengue virus. Virus titers obtained by TCID(50)-ELISA were comparable to those obtained by the plaque assay and by the traditional TCID(50)-cytopathic effect (CPE) test (TCID(50)-CPE), with a better reproducibility and a lower coefficient of variation. Quantitative comparison of TCID(50)-ELISA and TCID(50)-CPE resulted in a correlation coefficient of 0.976. Moreover, this new method showed a wider application to C6/36, Vero E6, BHK-21, and Vero cells compared with other titration methods. In summary, the novel TCID(50)-ELISA method described here provides a more reliable and more accurate alternative compared to the plaque assay and TCID(50)-CPE for titration of dengue virus.
authors.affiliation: Center for Clinical Laboratory, Zhujiang Hospital,Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Hu
authors.forename: Xi-xia
authors.initials: XX
authors.lastname: Ding
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.forename: Yu-xian
authors.initials: YX
authors.lastname: Pan
authors.forename: Li-wen
authors.initials: LW
authors.lastname: Qiu
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Che
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000254:growth & development* / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015203:Reproducibility of Results; D013997:Time Factors; D046509:Tissue Culture Techniques; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000276:immunology; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D017361:Viral Nonstructural Proteins