title: Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells.
abstract: Most clinically approved biomarkers of cancer are glycoproteins, and those residing on the cell surface are of particular interest in biotherapeutics. We report a method for selective labeling, affinity enrichment, and identification of cell-surface glycoproteins. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. We used mass spectrometry to identify over 70 cell surface glycoproteins and biochemically validated CD146 and integrin beta-4, both of which are known to promote metastatic behavior. These results establish cell-surface glycoproteomics as an effective technique for discovery of cancer biomarkers.
authors.affiliation: Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA.
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Hubbard
authors.forename: Michael
authors.initials: M
authors.lastname: Boyce
authors.forename: Cheryl T
authors.initials: CT
authors.lastname: McVaugh
authors.forename: Donna M
authors.initials: DM
authors.lastname: Peehl
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D020543:Proteome
chemical_list: D002241:Carbohydrates; D020543:Proteome