title: Bacterial expression and characterization of a novel human anti-IgE scFv fragment.
abstract: Antibodies highly specific to human immunoglobulin (Ig) E are capable of selectively blocking the IgE interaction or eliminating IgE-producing cells, thus providing valuable agents for diagnostics and treatment of various allergic illness. An example is omalizumab, a humanized monoclonal anti-IgE antibody that is approved for the treatment of patients with moderate-to-severe allergic diseases in the United States, European Union and other countries. Here, we describe the generation and characterization of a novel human anti-IgE as a single-chain antibody fragment (scFv). The bacterially-synthesized scFv showed high affinity (86 nM) and specificity to the Fc region of human IgE. To our knowledge, this is the first report of the production of a human anti-IgE scFv in E. coli. Its further development as a potential candidate for medical applications is discussed.
authors.affiliation: Center for Protein Engineering, Sichuan Industrial Institute of Antibiotics, Chengdu, Sichuan, China. mingrongw@yahoo.cn
authors.forename: MingRong
authors.initials: M
authors.lastname: Wang
authors.forename: YongXia
authors.initials: Y
authors.lastname: Zhang
authors.forename: TianFei
authors.initials: T
authors.lastname: Du
authors.forename: YunJian
authors.initials: Y
authors.lastname: Dai
authors.forename: YongZhi
authors.initials: Y
authors.lastname: He
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.forename: Cong
authors.initials: C
authors.lastname: Cong
authors.forename: MingYue
authors.initials: M
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000915:Antibody Affinity; D000918:Antibody Specificity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D057127:Single-Chain Antibodies; C026408:anti-IgE antibodies; D007073:Immunoglobulin E