title: Determination and pharmacokinetics of DT-13 in rat plasma by LC-MS.
abstract: A sensitive and specific LC-MS assay for DT-13 in rat plasma was developed. DT-13 is an active steroidal saponin present in Liriopes Radix and is developed as an anti-tumor drug candidate. The samples were extracted by acetonitrile-mediated plasma protein precipitation. The chromatographic separation was carried out using a Ultimate C(18) column (250 mm × 4.6 mm, i.d., 5 μm) with a mobile phase composed of acetonitrile: 5 mmol/L aqueous ammonium acetate (60:40, v:v). The method was validated and the specificity, linearity (r(2)=0.9980 within 10-1000 ng/mL), lower limit of quantitation (LLOQ, 10 ng/mL), precision (intra- and inter-day <12.3%), accuracy (93.4-106.3%), recovery (91.0 ± 4.7%) and stability were determined. The method was applied to the pharmacokinetic study of DT-13 in rat plasma after intravenous and intragastric administration. The results showed DT-13 underwent a prolonged absorption and slow elimination with a low oral bioavailability (5.51%) in rats.
authors.affiliation: Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China.
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Chen
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Yuan
authors.forename: Zheng-fang
authors.initials: ZF
authors.lastname: Hu
authors.forename: Jin
authors.initials: J
authors.lastname: Qi
authors.forename: Dan-ni
authors.initials: DN
authors.lastname: Zhu
authors.forename: Bo-yang
authors.initials: BY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D001682:Biological Availability; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000493:pharmacokinetics*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000097:blood* / Q000493:pharmacokinetics*; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D012503:Saponins