title: Imaging of intracellular acidic compartments with a sensitive rhodamine based fluorogenic pH sensor.
abstract: The parameters of intracellular acidic compartments are altered in apoptosis, autophagy, cancer metastasis, etc. Low background and selective staining of lysosomes and autophagosomes was achieved with N-(rhodamine 6G)-lactam-ethylenediamine (R6G-EDA) which fluoresces in acidic vesicles via pH mediated ring opening of the intramolecular lactam. Long retention time of R6G-EDA in lysosomes and its exceptional stability against photo-bleaching allow full time tracking of lysosome morphology changes in tumor necrosis factor-α (TNF-α) triggered cell death, suggesting its utility for acidic vesicle studies in cell biology.
authors.affiliation: Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Li
authors.forename: Shuqi
authors.initials: S
authors.lastname: Wu
authors.forename: Jiahuai
authors.initials: J
authors.lastname: Han
authors.forename: Shoufa
authors.initials: S
authors.lastname: Han
mesh_terms: D000818:Animals; D001343:Autophagy; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D012235:Rhodamines / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D014409:Tumor Necrosis Factor-alpha