title: Studies on erythrocytes in malaria infected blood sample with Raman optical tweezers.
abstract: Raman spectroscopy was performed on optically trapped red blood cells (RBCs) from blood samples of healthy volunteers (h-RBCs) and from patients suffering from P. vivax infection (m-RBCs). A significant fraction of m-RBCs produced Raman spectra with altered characteristics relative to h-RBCs. The observed spectral changes suggest a reduced oxygen-affinity or right shifting of the oxygen-dissociation curve for the intracellular hemoglobin in a significant fraction of m-RBCs with respect to its normal functional state.
authors.affiliation: Raja Ramanna Centre for Advanced Technology, Laser Biomedical Applications and Instrumentation Division, Indore-452013, India. raktim@rrcat.gov.in
authors.forename: Raktim
authors.initials: R
authors.lastname: Dasgupta
authors.forename: Ravi Shanker
authors.initials: RS
authors.lastname: Verma
authors.forename: Sunita
authors.initials: S
authors.lastname: Ahlawat
authors.forename: Abha
authors.initials: A
authors.lastname: Uppal
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Gupta
mesh_terms: D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood* / Q000469:parasitology*; D055620:Optical Phenomena; D052898:Optical Tweezers; D010100:Oxygen / Q000097:blood; D010966:Plasmodium vivax / Q000472:pathogenicity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*
chemical_list: D006454:Hemoglobins; D010100:Oxygen