title: Genetic map of artichoke × wild cardoon: toward a consensus map for Cynara cardunculus.
abstract: An integrated consensus linkage map is proposed for globe artichoke. Maternal and paternal genetic maps were constructed on the basis of an F(1) progeny derived from crossing an artichoke genotype (Mola) with its progenitor, the wild cardoon (Tolfa), using EST-derived SSRs, genomic SSRs, AFLPs, ten genes, and two morphological traits. For most genes, mainly belonging to the chlorogenic acid pathway, new markers were developed. Five of these were SNP markers analyzed through high-resolution melt technology. From the maternal (Mola) and paternal (Tolfa) maps, an integrated map was obtained, containing 337 molecular and one morphological markers ordered in 17 linkage groups (LGs), linked between Mola and Tolfa. The integrated map covers 1,488.8 cM, with an average distance of 4.4 cM between markers. The map was aligned with already existing maps for artichoke, and 12 LGs were linked via 31 bridge markers. LG numbering has been proposed. A total of 124 EST-SSRs and two genes were mapped here for the first time, providing a framework for the construction of a functional map in artichoke. The establishment of a consensus map represents a necessary condition to plan a complete sequencing of the globe artichoke genome.
authors.affiliation: Institute of Plant Genetics, National Research Council, Via Amendola, 165/A, 70126, Bari, Italy. gabriella.sonnante@igv.cnr.it
authors.forename: Gabriella
authors.initials: G
authors.lastname: Sonnante
authors.forename: Angela
authors.initials: A
authors.lastname: Gatto
authors.forename: Anita
authors.initials: A
authors.lastname: Morgese
authors.forename: Francesco
authors.initials: F
authors.lastname: Montemurro
authors.forename: Giulio
authors.initials: G
authors.lastname: Sarli
authors.forename: Emanuela
authors.initials: E
authors.lastname: Blanco
authors.forename: Domenico
authors.initials: D
authors.lastname: Pignone
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D031942:Cynara / Q000235:genetics*; D031943:Cynara scolymus / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D020224:Expressed Sequence Tags; D035264:Flowers; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D006579:Heterozygote; D008957:Models, Genetic; D018515:Plant Leaves; D010944:Plants / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D005819:Genetic Markers