title: Domestic cats convert [2H8]-β-carotene to [2H4]-retinol following a single oral dose.
abstract: Many animals convert β-carotene to retinol to meet their vitamin A (VA) requirement. However, this pathway is inefficient in many carnivores. This study quantified the plasma response to a single oral dose of [(2) H(8)]-β-carotene in adult domestic cats, including measurement of [(2) H(4)]-retinol derived from the dose. Cats were fed with either a control diet containing adequate VA (n = 5) or a VA-devoid diet (n = 5) for 28 days. An oral dose of either 5 mg/kg body weight (BW) (n = 4) or 10 mg/kg BW (n = 6) of [(2) H(8) ]-β-carotene was administered on day 28. Plasma samples were collected prior to dosing and at 6, 12, 24, 32, 48, 72, 120, 168 and 216 h post-dose. Plasma retinoids and β-carotene were measured using HPLC and [(2) H(4)]-retinol by GC-ECNCI-MS (gas chromatography/electron capture negative chemical ionization/mass spectrometry). β-carotene was undetectable in plasma prior to dosing. Post-dose, mean peak plasma β-carotene was 0.37 ± 0.06 nmol/ml at 9.0 ± 1.8 h following the dose, while [(2) H(4) ]-retinol peaked at 3.71 ± 0.69 pmol/ml at 55.2 ± 16.3 h. The ratio per cent of total area under the curve for [(2) H(4)]-retinol compared with the β-carotene response was 4.6 ± 2.6%. There was little effect of diet or dose on the β-carotene or [(2) H(4)]-retinol responses. The appearance of [(2) H(4)]-retinol in plasma indicates that cats are capable of converting β-carotene to active VA. Conversion efficiency was not calculated in this study, but it is likely inadequate to meet cats' VA requirement without the inclusion of preformed VA in the diet.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Green
authors.forename: G
authors.initials: G
authors.lastname: Tang
authors.forename: J
authors.initials: J
authors.lastname: Lango
authors.forename: K C
authors.initials: KC
authors.lastname: Klasing
authors.forename: A J
authors.initials: AJ
authors.lastname: Fascetti
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002415:Cats / Q000378:metabolism*; D004032:Diet / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D005260:Female; D013047:Specific Pathogen-Free Organisms; D014801:Vitamin A / Q000008:administration & dosage / Q000378:metabolism*; D019207:beta Carotene / Q000008:administration & dosage / Q000378:metabolism*
chemical_list: D019207:beta Carotene; D014801:Vitamin A