title: Effects of human amniotic fluid on costal cartilage regeneration (an experimental study).
abstract: OBJECTIVE
  After surgical correction of thoracic wall deformities, promoting neochondrogenesis in the perichondrial bed is very important for obtaining a flexible chest wall. In this experimental study, we aimed to investigate the effects of human amniotic fluid on cartilage regeneration in the costal perichondrial bed in a rabbit model.
  
  
  METHODS
  Fifty-four adult New Zealand rabbits were divided into three groups, with 18 rabbits in each group. The third and fifth costal cartilages were excised totally on the right side and partially excised on the left side in all groups. Group 1 served as controls. All rabbits in group 1 underwent closure of the perichondrium of the third costal cartilage and closure of the perichondrium of the fifth costal cartilage with reimplantation of reshaped cartilage into the fifth costal perichondrial bed. Rabbits in group 2 underwent closure of the perichondrium of the third and fifth costal cartilages after the administration of human amniotic fluid into the perichondrial bed. Group 3 rabbits received both human amniotic fluid and underwent cartilage reimplantation. The third and fifth costal perichondriums in group 3 rabbits were closed after the administration of human amniotic fluid and the reimplantation of reshaped cartilages. Rabbits were sacrificed at two, eight and 12 weeks after operation.
  
  
  RESULTS
  Numerical scores for the right perichondrial bed were significantly higher for group 2 compared to group 1 ( P < 0.05). But the difference was not significant for the left perichondrial bed ( P > 0.05). The diameter of chondrogenesis also did not differ significantly between left and right perichondrial bed for all groups.
  
  
  CONCLUSIONS
  Our study shows that administration of human amniotic fluid into the perichondrial bed increases chondrogenesis in adult rabbits, an important finding which may contribute to improving chest wall flexibility after the surgical correction of pectus excavatum.
authors.affiliation: Department of Thoracic Surgery, Gulhane Military Medical Academy, Ankara, Turkey. dr_kuthan_78@hotmail.com
authors.forename: K
authors.initials: K
authors.lastname: Kavakli
authors.forename: S
authors.initials: S
authors.lastname: Gurkok
authors.forename: H
authors.initials: H
authors.lastname: Caylak
authors.forename: O
authors.initials: O
authors.lastname: Genc
authors.forename: M
authors.initials: M
authors.lastname: Gamsizkan
authors.forename: O
authors.initials: O
authors.lastname: Yucel
authors.forename: E
authors.initials: E
authors.lastname: Karasahin
authors.forename: A
authors.initials: A
authors.lastname: Gozubuyuk
authors.forename: C
authors.initials: C
authors.lastname: Tasci
mesh_terms: D000653:Amniotic Fluid / Q000502:physiology*; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002356:Cartilage / Q000637:transplantation*; D002358:Cartilage, Articular / Q000502:physiology; D020219:Chondrogenesis / Q000502:physiology*; D004195:Disease Models, Animal; D006801:Humans; D011817:Rabbits; D019651:Plastic Surgery Procedures; D012272:Ribs / Q000601:surgery*; D014182:Transplantation, Autologous; D016896:Treatment Outcome