title: [Inhibition of glucoraphenin enzymolysis in Raphani Semen by processing].
abstract: OBJECTIVE
  To explore material basis of the pharmacological differences between the roasting and pro-roasting Raphani Semen.
  
  
  METHODS
  The two new sulfur-containing compounds (A209 and B221) were found changed after processing in the water decoction. The common precursor-C3 of A209 and B211 and the precursor of C3 were seperated and purified. Their transforming relationship was proved.
  
  
  RESULTS
  The result showed that glucosinolates could decompose into sulforaphane and transform into A209 and B221 further in the boiling process.
  
  
  CONCLUSIONS
  This study provides some experimental evidences for revealing the mechanism of Raphani Semen processing.
authors.affiliation: Shandong University of Traditional Chinese Medicine, Jinan 250355, China. luwenhaitcm@163.com
authors.forename: Wenhai
authors.initials: W
authors.lastname: Lv
authors.forename: Tao
authors.initials: T
authors.lastname: Ren
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Su
authors.forename: Xianghong
authors.initials: X
authors.lastname: Meng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005961:Glucosinolates / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D017879:Isothiocyanates; D012639:Seeds / Q000737:chemistry / Q000201:enzymology*; D013454:Sulfoxides; D013457:Sulfur Compounds / Q000737:chemistry*; D013861:Thiocyanates / Q000737:chemistry
chemical_list: C560518:A209 compound; C560519:B221 compound; D004365:Drugs, Chinese Herbal; D005961:Glucosinolates; D017879:Isothiocyanates; D013454:Sulfoxides; D013457:Sulfur Compounds; D013861:Thiocyanates; C522767:glucoraphenin; C016766:sulforaphane