title: Protective effect of the methanolic extract from Duchesnea indica against oxidative stress in vitro and in vivo.
abstract: Duchesnea indica (Rosaceae family) is herb used extensively in traditional Chinese medicine. In this study we investigated its protective activity against hydrogen peroxide (H(2)O(2))-induced cytotoxicity in human skin fibroblast (CCD-986Sk) cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced H(2)O(2) in the skin of hairless mice. Pretreatment of CCD-986Sk cells with methanolic extract of D. indica (DIM) improved the cell viability, enhanced activity of catalase, and decreased the leakage of lactate dehydrogenase (LDH) and the levels of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) in H(2)O(2) injured cells. Furthermore, DIM inhibited cell apoptosis and Bax expression induced by H(2)O(2). In addition, the level of H(2)O(2) stimulated by TPA was decreased by DIM in the skin of hairless mice. These results suggest that DIM offers protection against oxidative stress in vitro and in vivo, and this ability suggests potential use for protection against oxidation-induced skin damage.
authors.affiliation: Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, Chuncheon, Gangwon-Do 200-701, Republic of Korea.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Hu
authors.forename: Woong
authors.initials: W
authors.lastname: Han
authors.forename: Chaoqing
authors.initials: C
authors.lastname: Huang
authors.forename: Myeong-Hyeon
authors.initials: MH
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D000432:Methanol; D051379:Mice; D008812:Mice, Hairless; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D027824:Rosaceae / Q000737:chemistry*; D012867:Skin / Q000187:drug effects; D012997:Solvents; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D016877:Oxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D012997:Solvents; D051028:bcl-2-Associated X Protein; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013755:Tetradecanoylphorbol Acetate; D000432:Methanol