title: [Studies on anti-tumor metastatic constituents from Lindera glauca].
abstract: OBJECTIVE
  To study the anti-tumor metastatic constituents from Lindera glauca.
  
  
  METHODS
  Constituent isolation and purification was carried by repeated column chromatography (silica gel, Toyopearl HW-40 and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities.
  
  
  RESULTS
  Ten compounds (1 - 10) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: Laurotetanine (1), N-methyllaurotetanine (2), reticuline (3), pallidine (4), N-trans-feruloyltyramine (5), N-cis-feruloyltyramine (6), atheroline (7), norisosocorydine (8), [9,9,9-(2) H3]-(1S*, 3S*, 4S*, 8S*)-p-menthane-3,8-diol (9), [9,9,9-(2) H3 ]-(1S*, 3R*, 4S*, 8S*)-p-Menthane-3,8-diol (10). Compounds 1, 2, 4, 5, 7 and 9 showed positive anti-tumor metastatic activities,and compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.
  
  
  CONCLUSIONS
  Compound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from Lindera genus for the first time. Compounds 1, 4, and 5 showed significant anti-tumor metastatic activities.
authors.affiliation: School of Parmaceutical Sciences, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Wang
authors.forename: Shengan
authors.initials: S
authors.lastname: Tang
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhai
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification*; D001060:Aporphines / Q000737:chemistry / Q000302:isolation & purification; D044182:Benzylisoquinolines / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D027443:Lindera / Q000737:chemistry*; D039821:Monoterpenes / Q000737:chemistry / Q000302:isolation & purification; D009362:Neoplasm Metastasis / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D001060:Aporphines; D044182:Benzylisoquinolines; D039821:Monoterpenes; D010936:Plant Extracts; C001827:atheroline; C008256:laurotetanine; C003298:reticuline