title: c-erbB2 and c-myb induce mouse oocyte maturation involving activation of maturation promoting factor.
abstract: Proto-oncogenes are involved in cell growth, proliferation, and differentiation. In the present study, we investigated the roles and mediating pathways of proto-oncogenes c-erbB(2) and c-myb in mouse oocyte maturation by RT-PCR, real-time quantitative PCR, western blot, and recombinant proto-oncogene protein microinjection. Results showed that both c-erbB(2) and c-myb antisense oligodeoxynucleotides (c-erbB(2) ASODN and c-myb ASODN) inhibited germinal vesicle breakdown and the first polar body extrusion in a dose-dependent manner. However, microinjection of recombinant c-erbB(2) or c-myb protein into germinal vesicle stage oocytes stimulated oocyte meiotic maturation. In addition, the expression of c-erbB(2) and c-myb mRNA was detected in oocytes; and c-erbB(2) ASODN and c-myb ASODN inhibited c-erbB(2) mRNA and c-myb mRNA expression, respectively. Maturation promoting factor (MPF) inhibitor roscovitine did not affect the expression of c-erbB(2) mRNA and c-myb mRNA, but blocked the effects of recombinant c-erbB(2) and c-myb protein-induced oocyte maturation. Further, cyclin B1 protein expression in oocytes was remarkably inhibited by c-erbB(2) ASODN, c-myb ASODN, and roscovitine. Nonsense tat ODN had no effect on the expression of c-erbB(2), c-myb, and cyclin B1. These results suggest that c-erbB(2) and c-myb may induce oocyte maturation through mediating a pathway involving the activation of MPF.
authors.affiliation: Medical Experimental Teaching Department, Nanchang University, Nanchang, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Da-Lei
authors.initials: DL
authors.lastname: Zhang
authors.forename: Liang-Quang
authors.initials: LQ
authors.lastname: Xu
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Liu
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Zheng
mesh_terms: D000818:Animals; D004353:Drug Evaluation, Preclinical; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018734:Genes, erbB-2 / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D020570:Genes, myb / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D016200:Maturation-Promoting Factor / Q000235:genetics* / Q000378:metabolism; D000090204:Mesothelin; D051379:Mice; D008845:Microinjections; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects / Q000235:genetics; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D020598:Proto-Oncogene Proteins c-myb / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011687:Purines / Q000494:pharmacology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D000077546:Roscovitine; D015533:Transcriptional Activation
chemical_list: D016200:Maturation-Promoting Factor; D000090204:Mesothelin; D020319:Oligodeoxyribonucleotides, Antisense; D047428:Protein Kinase Inhibitors; D020598:Proto-Oncogene Proteins c-myb; D011687:Purines; D018719:Erb-b2 Receptor Tyrosine Kinases; D000077546:Roscovitine; C000715528:Msln protein, mouse