title: Adaptive optics for fluorescence wide-field microscopy using spectrally independent guide star and markers.
abstract: We describe the implementation and use of an adaptive optics loop in the imaging path of a commercial wide field microscope. We show that it is possible to maintain the optical performances of the original microscope when imaging through aberrant biological samples. The sources used for illuminating the adaptive optics loop are spectrally independent, in excitation and emission, from the sample, so they do not appear in the final image, and their use does not contribute to the sample bleaching. Results are compared with equivalent images obtained with an identical microscope devoid of adaptive optics system.
authors.affiliation: Laboratoire de Physique et d'Etude des Matériaux, École Supérieure de Physique et de Chimie Industrielles, Centre National de la Recherche Scientifique UMR 8213, Université Pierre et Marie Curie, 10 rue Vauquelin, Paris 75005,France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Vermeulen
authors.forename: Eleonora
authors.initials: E
authors.lastname: Muro
authors.forename: Thomas
authors.initials: T
authors.lastname: Pons
authors.forename: Vincent
authors.initials: V
authors.lastname: Loriette
authors.forename: Alexandra
authors.initials: A
authors.lastname: Fragola
mesh_terms: D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000706:statistics & numerical data; D055620:Optical Phenomena; D045663:Quantum Dots; D014404:Tubulin / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D014404:Tubulin