title: PKH26 as a fluorescent label for live human umbilical mesenchymal stem cells.
abstract: To determine whether PKH26 labeling affects the morphologies, phenotypes, proliferation, and secretion abilities of human umbilical mesenchymal stromal cells (HUMSCs) were investigated. Isolated HUMSCs were labeled with PKH26, and cell morphology was observed under microscope. Cell cycle, apoptotic cell death, expression of PKH26, and the proliferation rate were evaluated. Additionally, fluorescence intensity of PKH26 labeling at different passage times was quantified. There were no detectable differences in cell morphology, cell growth, and proliferation rate after PKH26 labeling. In addition, fluorescence intensity of PKH26 labeling was gradually reduced with increase of the passage times. The PKH26 labeling disappeared after passage six times. In summary, PKH26 labeling is a safe and effective way to label live HUMSCs.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Shao-Fang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hong-Tian
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhi-Nian
authors.forename: He
authors.initials: H
authors.lastname: Yuan-Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D009930:Organic Chemicals / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology*; D010641:Phenotype; D013194:Staining and Labeling / Q000379:methods; D014472:Umbilicus
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26