title: Liquid chromatographic method for the simultaneous determination of eprosartan and hydrochlorothiazide in tablets and human plasma.
abstract: A new, specific, and sensitive RP-HPLC method was developed for the simultaneous determination of eprosartan (EPR) and hydrochlorothiazide (HCT). Good chromatographic separation was achieved using a 250 x 4.6 mm id, 5 microm particle size Symmetry C18 column. The mobile phase acetonitrile-0.1 M phosphate buffer (35+65, v/v), pH 4.5, was pumped at a flow rate of 1 mL/min, with UV detection at 275 nm. The method showed good linearity in the ranges of 0.5-50 and 0.1-10 microg/mL, with LOD of 0.06 and 0.02 microg/mL and LOQ of 0.20 and 0.08 microg/mL for EPR and HCT, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their synthetic mixture and co-formulated tablets. The method was further extended to the in vitro and in vivo determination of the two drugs in spiked and real human plasma. Interference likely to be encountered from the co-administered drugs was studied.
authors.affiliation: University of Mansoura, Faculty of Pharmacy, Department of Analytical Chemistry, 35516, Mansoura, Egypt.
authors.forename: Fathalla
authors.initials: F
authors.lastname: Belal
authors.forename: Amina M
authors.initials: AM
authors.lastname: El-Brashy
authors.forename: Nahed
authors.initials: N
authors.lastname: El-Enany
authors.forename: Manar M
authors.initials: MM
authors.lastname: Tolba
mesh_terms: D000179:Acrylates / Q000097:blood* / Q000737:chemistry*; D000959:Antihypertensive Agents / Q000097:blood / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004338:Drug Combinations; D006801:Humans; D006852:Hydrochlorothiazide / Q000097:blood* / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000097:blood* / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000097:blood* / Q000737:chemistry*
chemical_list: D000179:Acrylates; D000959:Antihypertensive Agents; D004338:Drug Combinations; D007093:Imidazoles; D013876:Thiophenes; D006852:Hydrochlorothiazide; C068373:eprosartan