title: Immunolocalization of cytochrome P450 17alpha-hydroxylase/c17-20 lyase in the ovary of pregnant pigs and fetal gonads.
abstract: The study was designed to localize P450 17alpha-hydroxylase/c17-20 lyase (P450c17) in the ovaries of pregnant pigs and fetal gonads. Immunoexpression of P450c17 was investigated in the porcine ovaries (follicles and corpora lutea; CL) collected on days 10, 18, 32, 50, 70 and 90 post coitum (p.c.), and fetal gonads (testes and ovaries) on days 50, 70 and 90 p.c. The presence of P450c17 in ovarian follicles was demonstrated on all examined days of pregnancy but was restricted to theca interna cells. In CL, P450c17 was detected on all examined days of pregnancy but only in small luteal cells. In the female porcine fetuses, P450c17 immunostaining was found in oocyte nests and granulosa cells of primary ovarian follicles, while in the male fetuses in fetal Leydig cells. In conclusion, the immunolocalization of P450c17, detected in the ovaries of pregnant pigs and fetal porcine gonads, indicates the potential sites of androgen synthesis. We suggest that androgens may play a role in the maintenance of pregnancy and in the development of prenatal gonads in pigs.
authors.affiliation: Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. katarzyna.knapczyk@uj.edu.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Knapczyk-Stwora
authors.forename: Magdalena
authors.initials: M
authors.lastname: Ciechanowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Sternak
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Durlej
authors.forename: Maria
authors.initials: M
authors.lastname: Słomczyńska
mesh_terms: D000728:Androgens / Q000378:metabolism; D000818:Animals; D005260:Female; D005333:Fetus / Q000201:enzymology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D010053:Ovary / Q000196:embryology / Q000201:enzymology*; D011247:Pregnancy; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000196:embryology / Q000378:metabolism*; D013737:Testis / Q000196:embryology / Q000201:enzymology
chemical_list: D000728:Androgens; D013254:Steroid 17-alpha-Hydroxylase