title: Novel biodegradable polycaprolactone occlusion device combining nanofibrous PLGA/collagen membrane for closure of atrial septal defect (ASD).
abstract: The purpose of this report was to develop novel biodegradable occlusion devices for closure of atrial septal defects (ASD). To manufacture the biodegradable occluders, polycaprolactone (PCL) components were first fabricated by a lab-scale micro-injection molding machine. They were then assembled and hot-spot welded into double umbrella-like devices of 50 mm in diameter. A special mechanism at the axis of the occluder was designed to self-lock the occluder after the two umbrellas were expanded. Furthermore, a nanofibrous matrix of poly-D-L-lactide-glycolide (PLGA)/type I collagen blend was produced via electrospinning to develop biodegradable and biomimetic anti-shunt membranes for the occluders. Characterization of the biodegradable PCL occluders was carried out. PCL occluders exhibited mechanical properties comparable to that of commercially available Amplatzer occluders. The sealing capability of biodegradable occluders was found superior to that of Amplatzer occluders. In addition, the cell attachment and spreading of endothelial cells seeded on the PLGA/collagen nanofibrous matrix and the interaction between cells and PLGA/collagen nanofibers were studied. The nanofibrous membranes made of PLGA/collagen were very effective in promoting cell proliferation during culture.
authors.affiliation: Biomaterials Lab, Mechanical Engineering, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan. shihjung@mail.cgu.edu.tw
authors.forename: Shih-Jung
authors.initials: SJ
authors.lastname: Liu
authors.forename: Kang-Ming
authors.initials: KM
authors.lastname: Peng
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Hsiao
authors.forename: Kuo-Sheng
authors.initials: KS
authors.lastname: Liu
authors.forename: Hung-Tao
authors.initials: HT
authors.lastname: Chung
authors.forename: Jan-Kan
authors.initials: JK
authors.lastname: Chen
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D006344:Heart Septal Defects, Atrial / Q000628:therapy*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D055989:Septal Occluder Device; D013314:Stress, Mechanical
chemical_list: D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen