title: Synthesis and functionalization of persistent luminescence nanoparticles with small molecules and evaluation of their targeting ability.
abstract: We have recently reported the design and use of inorganic nanoparticles with persistent luminescence properties. Such nanoparticles can be excited with a UV lamp for 2min and emit light in the near-infrared area for dozen of minutes without any further excitation. This property is of particular interest for small animal optical imaging, since it avoids the autofluorescence of endogenous fluorophores which is one major problem encountered when using fluorescent probes. We report herein the synthesis of persistent luminescence nanoparticles (PLNPs) and their functionalization with two small targeting molecules: biotin and Rak-2. We provide characterization of each PLNP as well as preliminary evidence of the ability of PLNP-PEG-Biotin to target streptavidin and PLNP-PEG-Rak-2 to bind prostate cancer cells in vitro.
authors.affiliation: Unité de Pharmacologie Chimique et Génétique et d'Imagerie, CNRS, UMR 8151, F-75270 Paris Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Maldiney
authors.forename: Gerardo
authors.initials: G
authors.lastname: Byk
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wattier
authors.forename: Johanne
authors.initials: J
authors.lastname: Seguin
authors.forename: Raz
authors.initials: R
authors.lastname: Khandadash
authors.forename: Michel
authors.initials: M
authors.lastname: Bessodes
authors.forename: Cyrille
authors.initials: C
authors.lastname: Richard
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
mesh_terms: D001710:Biotin / Q000737:chemistry; D002122:Calcium Chloride / Q000737:chemistry; D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D002712:Chlorides / Q000008:administration & dosage / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D004419:Dysprosium / Q000008:administration & dosage / Q000737:chemistry; D005063:Europium / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D049408:Luminescent Agents / Q000008:administration & dosage* / Q000737:chemistry; D017616:Magnesium Compounds / Q000737:chemistry; D008297:Male; D017895:Manganese Compounds / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009566:Nitrates / Q000008:administration & dosage / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011471:Prostatic Neoplasms; D011485:Protein Binding; D012821:Silanes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D019809:Streptavidin / Q000737:chemistry; D013499:Surface Properties; D013818:Thermogravimetry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D002712:Chlorides; D049408:Luminescent Agents; D017616:Magnesium Compounds; D017895:Manganese Compounds; D009566:Nitrates; D012821:Silanes; D017967:Zinc Compounds; C077424:dysprosium nitrate; D004419:Dysprosium; D011092:Polyethylene Glycols; C040733:tetraethoxysilane; D005063:Europium; D001710:Biotin; C018330:magnesium nitrate; C016837:zinc chloride; D019809:Streptavidin; D002122:Calcium Chloride; C025340:manganese chloride