title: Amplified fragment length polymorphism analysis of Listeria monocytogenes by Experion™ automated microfluidic electrophoresis.
abstract: Fifty Listeria monocytogenes strains were genotyped by sAFLP and PCR products were separated by agarose gel and automated chip-based microfluidic electrophoresis. A high congruency of results was observed comparing the two techniques, although for some cultures a better separation of sAFLP fragments was achieved with microfluidic system, which proved to be a highly reliable and reproducible tool to improve the molecular typing of L. monocytogenes, requiring lower volumes of samples and reducing significantly analysis time.
authors.affiliation: Department of Health Sciences, Faculty of Medicine and Surgery, University of Molise, Campobasso, Italy.
authors.forename: Michela Lucia
authors.initials: ML
authors.lastname: Sammarco
authors.forename: Monia
authors.initials: M
authors.lastname: Vitullo
authors.forename: Manuela
authors.initials: M
authors.lastname: Tamburro
authors.forename: Mirella
authors.initials: M
authors.lastname: Pontello
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Ripabelli
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000295:instrumentation / Q000379:methods*; D057205:Automation, Laboratory; D001426:Bacterial Proteins / Q000235:genetics; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification / Q000235:genetics*; D008088:Listeriosis / Q000382:microbiology; D058889:Molecular Typing / Q000295:instrumentation / Q000379:methods*; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins; C069761:internalin protein, Bacteria