title: Mass transport at rotating disk electrodes: effects of synthetic particles and nerve endings.
abstract: An unstirred layer (USL) exists at the interface of solids with solutions. Thus, the particles in brain tissue preparations possess a USL as well as at the surface of a rotating disk electrode (RDE) used to measure chemical fluxes. Time constraints for observing biological kinetics based on estimated thicknesses of USLs at the membrane surface in real samples of nerve endings were estimated. Liposomes, silica, and Sephadex were used separately to model the tissue preparation particles. Within a solution stirred by the RDE, both diffusion and hydrodynamic boundary layers are formed. It was observed that the number and size of particles decreased the following: the apparent diffusion coefficient excluding Sephadex, boundary layer thicknesses excluding silica, sensitivity excluding diluted liposomes (in agreement with results from other laboratories), limiting current potentially due to an increase in the path distance, and mixing time. They have no effect on the detection limit (6 ± 2 nM). The RDE kinetically resolves transmembrane transport with a timing of approximately 30 ms.
authors.affiliation: Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Chiu
authors.forename: Peter A
authors.initials: PA
authors.lastname: Lukus
authors.forename: Jamie L
authors.initials: JL
authors.lastname: Doyle
authors.forename: James O
authors.initials: JO
authors.lastname: Schenk
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D003911:Dextrans / Q000737:chemistry; D004058:Diffusion; D004566:Electrodes; D066298:In Vitro Techniques; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D009411:Nerve Endings / Q000737:chemistry / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000737:chemistry; D012996:Solutions
chemical_list: D003911:Dextrans; D008081:Liposomes; D012996:Solutions; D012822:Silicon Dioxide; C025614:sephadex