title: Poly(methyl malate) nanoparticles: formation, degradation, and encapsulation of anticancer drugs.
abstract: PMLA nanoparticles with diameters of 150-250 nm are prepared, and their hydrolytic degradation is studied under physiological conditions. Degradation occurs by hydrolysis of the side chain methyl ester followed by cleavage of the main-chain ester group with methanol and L-malic acid as the final degradation products. No alteration of the cell viability is found after 1 h of incubation, but toxicity increases significantly after 3 d, probably due to the noxious effect of the released methanol. Anticancer drugs temozolomide and doxorubicin are encapsulated in the NPs with 20-40% efficiency, and their release is monitored using in vitro essays. Temozolomide is fully liberated within several hours, whereas doxorubicin is steadily released from the particles over a period of 1 month.
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, ETSEIB, Diagonal 647, 08028 Barcelona, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Lanz-Landázuri
authors.forename: Montserrat
authors.initials: M
authors.lastname: García-Alvarez
authors.forename: José
authors.initials: J
authors.lastname: Portilla-Arias
authors.forename: Antxon Martínez
authors.initials: AM
authors.lastname: de Ilarduya
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Patil
authors.forename: Eggehard
authors.initials: E
authors.lastname: Holler
authors.forename: Julia Y
authors.initials: JY
authors.lastname: Ljubimova
authors.forename: Sebastián
authors.initials: S
authors.lastname: Muñoz-Guerra
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D004339:Drug Compounding / Q000379:methods*; D004655:Emulsions; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000138:chemical synthesis* / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011208:Powders; D013010:Sonication; D000077204:Temozolomide; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D004655:Emulsions; D008293:Malates; D011091:Polyesters; D011108:Polymers; D011208:Powders; C569743:poly(alpha-methyl-beta-malate); C074640:poly(malate); D003606:Dacarbazine; D004317:Doxorubicin; D000077204:Temozolomide