title: Angiotensin-converting enzyme inhibitory activity in Mexican Fresco cheese.
abstract: The objective of this study was to evaluate if Mexican Fresco cheese manufactured with specific lactic acid bacteria (LAB) presented angiotensin I-converting enzyme inhibitory (ACEI) activity. Water-soluble extracts (3 kDa) obtained from Mexican Fresco cheese prepared with specific LAB (Lactococcus, Lactobacillus, Enterococcus, and mixtures: Lactococcus-Lactobacillus and Lactococcus-Enterococcus) were evaluated for ACEI activity. Specific peptide fractions with high ACEI were analyzed using reverse phase-HPLC coupled to mass spectrometry for determination of amino acid sequence. Cheese containing Enterococcus faecium or a Lactococcus lactis ssp. lactis-Enterococcus faecium mixture showed the largest number of fractions with ACEI activity and the lowest half-maximal inhibitory concentration (IC(50); <10 μg/mL). Various ACEI peptides derived from β-casein [(f(193-205), f(193-207), and f(193-209)] and α(S1)-casein [f(1-15), f(1-22), f(14-23), and f(24-34)] were found. The Mexican Fresco cheese manufactured with specific LAB strains produced peptides with potential antihypertensive activity.
authors.affiliation: Laboratorio de Química y Biotecnología de Productos Lácteos Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: M J
authors.initials: MJ
authors.lastname: Torres-Llanez
authors.forename: A F
authors.initials: AF
authors.lastname: González-Córdova
authors.forename: A
authors.initials: A
authors.lastname: Hernandez-Mendoza
authors.forename: H S
authors.initials: HS
authors.lastname: Garcia
authors.forename: B
authors.initials: B
authors.lastname: Vallejo-Cordoba
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D002611:Cheese / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling / Q000379:methods; D008894:Milk Proteins / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D008894:Milk Proteins