title: Gel-based phosphoproteomics analysis of sarcoplasmic proteins in postmortem porcine muscle with pH decline rate and time differences.
abstract: Meat quality development is highly influenced by the pH decline caused by the postmortem (PM) glycolysis. Protein phosphorylation is an important mechanism in regulating the activity of glycometabolic enzymes. Here, a gel-based phosphoproteomic study was performed to analyze the protein phosphorylation in sarcoplasmic proteins from three groups of pigs with different pH decline rates from PM 1 to 24 h. Globally, the fast pH decline group had the highest phosphorylation level at PM 1 h, but lowest at 24 h, whereas the slow pH decline group showed the reverse case. The same pattern was also observed in most individual bands in 1-DE. The protein phosphorylation levels of 12 bands were significantly affected by the synergy effects of pH and time (p<0.05). Protein identification revealed that most of the phosphoproteins were glycometabolism-related enzymes, and the others were involved in stress response, phosphocreatine metabolism, and other functions. The phosphorylation of pyruvate kinase and triosephosphate isomerase-1 showed to be related to PM muscle pH decline rate. Our work sheds light on the potential role of protein phosphorylation on regulating meat quality development.
authors.affiliation: Department of Food Science, Faculty of Life Sciences, University of Copenhagen, Denmark. rla@life.ku.dk
authors.forename: Honggang
authors.initials: H
authors.lastname: Huang
authors.forename: Martin R
authors.initials: MR
authors.lastname: Larsen
authors.forename: Anders H
authors.initials: AH
authors.lastname: Karlsson
authors.forename: Luigi
authors.initials: L
authors.lastname: Pomponio
authors.forename: Leonardo Nanni
authors.initials: LN
authors.lastname: Costa
authors.forename: René
authors.initials: R
authors.lastname: Lametsch
mesh_terms: D000818:Animals; D018130:Diamond / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000378:metabolism*; D010766:Phosphorylation; D011180:Postmortem Changes; D040901:Proteomics; D011786:Quality Control; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling; D013552:Swine; D013997:Time Factors
chemical_list: D018130:Diamond