title: Determination of trace gastrin and diagnosis of human diseases using CdTe quantum dots labelled gastrin antibodies as phosphorescence sensors.
abstract: CdTe quantum dots (CdTe-QDs) can emit strong and stable room temperature phosphorescence (RTP) via the perturbation effect of a Pb(2+) ion on the surface of a nitrocellulose membrane (NCM). CdTe-QDs-Ab(GAS), the product of CdTe-QDs labelled gastrin antibodies (Ab(GAS)), can not only maintain good RTP characteristics, but can also be used as a RTP sensor and carry out highly specific immunoreactions with gastrin (GAS) to form GAS-Ab(GAS)-CdTe-QDs causing the ΔI(p) of the system to sharply enhance. Thus, a new solid substrate room temperature phosphorescence immunoassay (SSRTPIA) for the determination of GAS was established based on the linear relativity between the ΔI(p) of the system and the content of GAS. The limit of quantification (LOQ) of this method was 0.43 fg spot(-1) with the corresponding concentration being 1.1 × 10(-12) g mL(-1) and sampling quantity being 0.40 per spot(-1). This highly specific, accurate, selective and sensitive RTP sensor has been applied to the determination of GAS in biological samples and the diagnosis of diseases, and the results agreed well with those obtained by radioimmunometric assay (RIA). Meanwhile, the mechanism of SSRTPIA for the determination of GAS using CdTe-QDs-Ab(GAS) as the RTP sensor was discussed.
authors.affiliation: Fujian Zhangzhou Normal College, Zhangzhou Institute of Technology, Fujian Education College, Zhangzhou, Fuzhou, China. zzsyliujiaming@163.com
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Huang
authors.forename: Shaoqin
authors.initials: S
authors.lastname: Lin
authors.forename: Wenlian
authors.initials: W
authors.lastname: Cai
authors.forename: Changqing
authors.initials: C
authors.lastname: Lin
authors.forename: LiHong
authors.initials: L
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Lin
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Li
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D005755:Gastrins / Q000097:blood* / Q000276:immunology; D005767:Gastrointestinal Diseases / Q000175:diagnosis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D045663:Quantum Dots; D013691:Tellurium / Q000737:chemistry*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D019187:Cadmium Compounds; D005755:Gastrins; D013691:Tellurium; C028337:cadmium telluride