title: [Screen anti-influenza virus serum marker of Lonicera japonica by proteomics technology].
abstract: OBJECTIVE
  The candidate anti-influenza virus serum marker of Lonicera japonica was searched by comparing the serum protein discrepancy of experimental groups. It will provide the basis for setting forth the action and mechanism of anti-influenza virus.
  
  
  METHODS
  Two-dimensional electrophoresis (2-DE) was used to separate different experimental groups and the differences in serum protein was compared. The significantly expressed protein spots was selected for mass spectrum identification and bioinformatics analysis.
  
  
  RESULTS
  Fifteen identified points of protein have more typical differences between experimental groups. The structures were identified by MALDI-TOF-MS, and 12 functional protein component ownership were retrieved.
  
  
  CONCLUSIONS
  There was significant difference between the serum protein of experimental groups Proteomic methods can be used to select and identify the serum marker. It is expected to clarify the mechanism of anti-influenza virus of L. japonica.
authors.affiliation: Shandong Academy of Traditional Chinese Medicine, Jinan 250014, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Junying
authors.initials: J
authors.lastname: Shi
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000097:blood* / Q000302:isolation & purification* / Q000494:pharmacology; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D031239:Lonicera / Q000737:chemistry*; D008297:Male; D051379:Mice; D009975:Orthomyxoviridae / Q000187:drug effects*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000998:Antiviral Agents; D015415:Biomarkers; D001798:Blood Proteins