title: [The influence of GABAA receptor on the analgesic action of intrathecally injected oxysophoridine].
abstract: .This study is to investigate the analgesic effect produced by intrathecal injection (ith) of oxysophoridine (OSR) and the mechanism of GABAA receptor. Warm water tail-flick test was used to detect the analgesic effect of OSR (12.5, 6.25, and 3.13 mg.kg-1 ith) and to observe the influence of GABA (gamma aminobutyric acid) agonist or antagonist on the analgesic effect of OSR in mice. Immunohistochemistry method were used to detect the influence of OSR (12.5 mg.kg-1, ith) on the GABAARalpha1 protein expression in spinal cord. The results obtained covers that OSR (12.5 and 6.25 mg.kg-, ith) alleviates pain significantly with the warm water tail-flick test (P<0.05, P<0.01), the rate of pain threshold increases by 68.45%; GABA and muscimol (MUS) produces analgesic synergism together with the OSR, picrotoxin (PTX) and bicuculline (BIC) antagonize the analgesic effect of OSR; OSR (12.5 mg.kg-1, ith) significantly increase the positive number of GABAARalpha1 nerve cell in spinal cord (P<0.01) and significantly decrease the average grey levels (P<0.01). In conclusion, OSR intrathecal injection has significant analgesic effect. And GABAA receptor in spinal cord is involved in the analgesic mechanism.
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Ningxia Engineering & Technology Research Center for Modernization of Hui Medicine, Yinchuan 750004, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Jin-xian
authors.initials: JX
authors.lastname: Gao
authors.forename: Zheng-hong
authors.initials: ZH
authors.lastname: Yi
authors.forename: Lin
authors.initials: L
authors.lastname: Yan
authors.forename: Yuan-Xu
authors.initials: YX
authors.lastname: Jiang
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D005260:Female; D058785:GABA-A Receptor Agonists / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D009118:Muscimol / Q000494:pharmacology; D017288:Pain Threshold / Q000187:drug effects*; D010852:Picrotoxin / Q000494:pharmacology; D011897:Random Allocation; D011963:Receptors, GABA-A / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000700:Analgesics; D001640:Bicuculline; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D009118:Muscimol; D010852:Picrotoxin; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; C546240:GABRA1 protein, human; C406983:oxysophoridine