title: Hyperinsulinemia induces hypertension associated with neurogenic vascular dysfunction resulting from abnormal perivascular innervations in rat mesenteric resistance arteries.
abstract: We previously reported that chronic hyperinsulinemia and insulin resistance induced by fructose-drinking loading elicited hypertension associated with abnormal neuronal regulation of vascular tone in an in vivo study using pithed rats. Therefore, to further clarify the detailed mechanisms of perivascular nervous system malfunction induced by chronic hyperinsulinemia, we investigated the neurogenic vascular responses and distribution of perivascular nerves using mesenteric vascular beds isolated from fructose-loaded rats with hyperinsulinemia. Male Wistar rats (6 weeks old) received 15% fructose solution as drinking fluid for 10 weeks (fructose-drinking rats, FDR), which resulted in significant increases in plasma levels of insulin, the glucose-insulin index, blood norepinephrine (NE) levels and systolic blood pressure, but not blood glucose levels, when compared with normal water-drinking rats (control rats). In perfused mesenteric vascular beds of FDR, enhanced adrenergic nerve-mediated vasoconstriction with no effect on NE-induced vasoconstriction and decreased calcitonin gene-related peptide (CGRP)-containing nerve-mediated vasodilation with no effect on CGRP-induced vasodilation were observed. Immunohistochemistry studies showed increased density of neuropeptide Y immunopositive adrenergic fibers and reduced density of CGRP immunopositive fibers in mesenteric arteries of FDR. Furthermore, FDR showed decreased CGRP content in dorsal root ganglia. These findings suggest that dysfunction of the neuronal vascular control system resulting from abnormal innervation of mesenteric perivascular nerves induced by the hyperinsulinemic state is responsible for the development of hypertension in FDR.
authors.affiliation: Department of Clinical Pharmaceutical Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Zamami
authors.forename: Shingo
authors.initials: S
authors.lastname: Takatori
authors.forename: Narumi
authors.initials: N
authors.lastname: Hobara
authors.forename: Nana
authors.initials: N
authors.lastname: Yabumae
authors.forename: Panot
authors.initials: P
authors.lastname: Tangsucharit
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Naoya
authors.initials: N
authors.lastname: Hashikawa
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Kenji
authors.initials: K
authors.lastname: Sasaki
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kawasaki
mesh_terms: D059331:Adrenergic Neurons / Q000502:physiology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000494:pharmacology; D004195:Disease Models, Animal; D005632:Fructose / Q000009:adverse effects; D005727:Ganglia, Spinal / Q000378:metabolism; D006946:Hyperinsulinism / Q000097:blood / Q000139:chemically induced / Q000150:complications*; D006973:Hypertension / Q000097:blood / Q000209:etiology* / Q000503:physiopathology; D007328:Insulin / Q000097:blood; D008297:Male; D008638:Mesenteric Arteries / Q000294:innervation* / Q000503:physiopathology*; D009638:Norepinephrine / Q000097:blood / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013564:Sympathetic Nervous System / Q000378:metabolism / Q000503:physiopathology*; D014655:Vascular Resistance / Q000502:physiology*; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
chemical_list: D001786:Blood Glucose; D007328:Insulin; D005632:Fructose; D015740:Calcitonin Gene-Related Peptide; D009638:Norepinephrine