title: Direct electrochemical reduction of graphene oxide on ionic liquid doped screen-printed electrode and its electrochemical biosensing application.
abstract: A novel electrochemical biosensing platform using electrochemically reduced graphene oxide (ER-GNO) modified electrode was proposed. This modified electrode was prepared by one-step electrodeposition of the exfoliated GNO sheets onto the ionic liquid doped screen-printed electrode (IL-SPE). The resulting ER-GNO/IL-SPE brought new capabilities for electrochemical devices by combining the advantages of ER-GNO and disposable electrode. Two important biomolecules, nicotinamide adenine dinucleotide (NADH) and hydrogen peroxide (H(2)O(2)), were employed to study the electrochemical performance of the ER-GNO/IL-SPE, which exhibited more favorable electron transfer kinetics than the bare IL-SPE. On the basis of the greatly enhanced electrochemical reactivity of H(2)O(2) at the developed electrode, ER-GNO and glucose oxidase constructed disposable biosensor showed better analytical performance for the glucose detection compared with the IL-SPE based biosensor. The linear range for the detection of glucose was from 5.0 μM to 12.0 mM with a detection limit of 1.0 μM. This work provides a useful avenue for implementing ER-GNO as a new generation of electrochemical transducer in disposable electrode, which could expand the scope of graphene constructed electrochemical biosensing devices and hold great promise for routine sensing applications.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Ping
authors.forename: Yixian
authors.initials: Y
authors.lastname: Wang
authors.forename: Kai
authors.initials: K
authors.lastname: Fan
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Yibin
authors.initials: Y
authors.lastname: Ying
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005947:Glucose / Q000032:analysis; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D009243:NAD / Q000737:chemistry; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
chemical_list: D052578:Ionic Liquids; D010087:Oxides; D009243:NAD; D006108:Graphite; D006861:Hydrogen Peroxide; D005947:Glucose