title: Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species.
abstract: The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed based on two F(2) mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups, except for intergenomic T6A(t)S.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes were reduced compared with the B, and G genome chromosomes.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Lavrentieva Ave. 10, Novosibirsk, 630090, Russia. oxanad@bionet.nsc.ru
authors.forename: O
authors.initials: O
authors.lastname: Dobrovolskaya
authors.forename: C
authors.initials: C
authors.lastname: Boeuf
authors.forename: J
authors.initials: J
authors.lastname: Salse
authors.forename: C
authors.initials: C
authors.lastname: Pont
authors.forename: P
authors.initials: P
authors.lastname: Sourdille
authors.forename: M
authors.initials: M
authors.lastname: Bernard
authors.forename: E
authors.initials: E
authors.lastname: Salina
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D005819:Genetic Markers; D005821:Genetic Techniques; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D008957:Models, Genetic; D010802:Phylogeny; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D004247:DNA