title: Oil body proteins sequentially accumulate throughout seed development in Brassica napus.
abstract: Despite the importance of seed oil bodies (OBs) as enclosed compartments for oil storage, little is known about lipid and protein accumulation in OBs during seed formation. OBs from rapeseed (Brassica napus) consist of a triacylglycerol (TAG) core surrounded by a phospholipid monolayer embedded with integral proteins which confer high stability to OBs in the mature dry seed. In the present study, we investigated lipid and protein accumulation patterns throughout seed development (from 5 to 65 days after pollination [DAP]) both in the whole seed and in purified OBs. Deposition of the major proteins (oleosins, caleosins and steroleosins) into OBs was assessed through (i) gene expression pattern, (ii) proteomics analysis, and (iii) protein immunodetection. For the first time, a sequential deposition of integral OB proteins was established. Accumulation of oleosins and caleosins was observed starting from early stages of seed development (12-17 DAP), while steroleosins accumulated later (~25 DAP) onwards.
authors.affiliation: INRA, AgroParisTech, Institut Jean-Pierre Bourgin, UMR 1318, F-78000 Versailles, France. Pascale.Jolivet@versailles.inra.fr
authors.forename: Pascale
authors.initials: P
authors.lastname: Jolivet
authors.forename: Céline
authors.initials: C
authors.lastname: Boulard
authors.forename: Annick
authors.initials: A
authors.lastname: Bellamy
authors.forename: Benoît
authors.initials: B
authors.lastname: Valot
authors.forename: Sabine
authors.initials: S
authors.lastname: d'Andréa
authors.forename: Michel
authors.initials: M
authors.lastname: Zivy
authors.forename: Nathalie
authors.initials: N
authors.lastname: Nesi
authors.forename: Thierry
authors.initials: T
authors.lastname: Chardot
mesh_terms: D029688:Brassica napus / Q000254:growth & development / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015870:Gene Expression; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D005227:Fatty Acids; D010743:Phospholipids; D010938:Plant Oils; D010940:Plant Proteins; D014280:Triglycerides; C402836:caleosin protein, Sesamum indicum; C079356:oleosin protein, Brassica napus