title: [Human arginase I from the recombinant yeast Hansenula polymorpha: isolation and characterization of the enzyme].
abstract: Purified human arginase I preparations homogeneous in SDS-PAAG test were obtained by the affinity chromatography on the synthesized sorbent L-arginine-macroporous glass. Some physico-chemical characteristics of the isolated arginase preparation have been estimated: thermo- and pH-stability, temperature- and pH-optima of the enzyme. The influence of some bivalent metal ions and other additives on enzymatic activity for stabilization of the enzyme and optimization of its storage conditions was studied.
authors.forename: N Ie
authors.initials: NIe
authors.lastname: Stasiuk
authors.forename: G Z
authors.initials: GZ
authors.lastname: Gaîda
authors.forename: Ie P
authors.initials: IeP
authors.lastname: Koval'chuk
authors.forename: O V
authors.initials: OV
authors.lastname: Stasyk
authors.forename: M V
authors.initials: MV
authors.lastname: Gonchar
mesh_terms: D000818:Animals; D001119:Arginase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D001120:Arginine / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005898:Glass; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D010843:Pichia / Q000235:genetics; D016062:Porosity; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D008670:Metals; D011994:Recombinant Proteins; D001120:Arginine; D001119:Arginase; D005990:Glycerol