title: Repression of gibberellin biosynthesis or signaling produces striking alterations in poplar growth, morphology, and flowering.
abstract: We modified gibberellin (GA) metabolism and signaling in transgenic poplars using dominant transgenes and studied their effects for 3 years under field conditions. The transgenes that we employed either reduced the bioactive GAs, or attenuated their signaling. The majority of transgenic trees had significant and in many cases dramatic changes in height, crown architecture, foliage morphology, flowering onset, floral structure, and vegetative phenology. Most transgenes elicited various levels of height reduction consistent with the roles of GA in elongation growth. Several other growth traits were proportionally reduced, including branch length, internode distance, and leaf length. In contrast to elongation growth, stem diameter growth was much less affected, suggesting that semi-dwarf trees in dense stands might provide high levels of biomass production and carbon sequestration. The severity of phenotypic effects was strongly correlated with transgene expression among independent transgenic events, but often in a non-linear manner, the form of which varied widely among constructs. The majority of semi-dwarfed, transgenic plants showed delayed bud flush and early bud set, and expression of a native GAI transgene accelerated first time flowering in the field. All of the phenotypic changes observed in multiple years were stable over the 3 years of field study. Our results suggest that transgenic modification of GA action may be useful for producing semi-dwarf trees with modified growth and morphology for horticulture and other uses.
authors.affiliation: School of Forest Research and Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Zawaski
authors.forename: Mahita
authors.initials: M
authors.lastname: Kadmiel
authors.forename: Jim
authors.initials: J
authors.lastname: Pickens
authors.forename: Cathleen
authors.initials: C
authors.lastname: Ma
authors.forename: Steven
authors.initials: S
authors.lastname: Strauss
authors.forename: Victor
authors.initials: V
authors.lastname: Busov
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D005875:Gibberellins / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D032107:Populus / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D025341:Principal Component Analysis; D017434:Protein Structure, Tertiary / Q000235:genetics / Q000502:physiology; D012044:Regression Analysis; D015398:Signal Transduction / Q000502:physiology*; D019076:Transgenes / Q000235:genetics; D014197:Trees
chemical_list: D005875:Gibberellins; D010940:Plant Proteins; D006899:Mixed Function Oxygenases; C121463:gibberellin 2-dioxygenase