title: Lectins for histochemical demonstration of glycans.
abstract: Lectins have been proven to be invaluable reagents for the histochemical detection of glycans in cells and tissues by light and electron microscopy. This technical review deals with the conditions of tissue fixation and embedding for lectin labeling, as well as various markers and related labeling techniques. Furthermore, protocols for lectin labeling of sections from paraffin and resin-embedded tissues are detailed together with various controls to demonstrate the specificity of the labeling by lectins.
authors.affiliation: Department of Integrated OMICS for Biomedical Science, WCU Program of Yonsei University Graduate School, Advanced Science and Technology Center, Yonsei University, 134 Shinchon-dong, Seodaenum-gu, Seoul 120-749, Korea. jurgen.roth@yonsei.ac.kr
authors.forename: Jürgen
authors.initials: J
authors.lastname: Roth
mesh_terms: D004076:Digoxigenin; D017979:Gold Colloid; D006651:Histocytochemistry / Q000379:methods*; D037102:Lectins / Q000737:chemistry*; D008854:Microscopy, Electron / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D017979:Gold Colloid; D037102:Lectins; D011134:Polysaccharides; D004076:Digoxigenin