title: Evaluation of cellular influences of platinum nanoparticles by stable medium dispersion.
abstract: Platinum nanoparticles have industrial application, for example in catalysis, and are used in consumer products such as cosmetics and supplements. Therefore, among the many nanoparticles, platinum is one of the more accessible nanoparticles for consumers. Most platinum nanoparticles that are used in cosmetics and supplements which have an anti-oxidant activity are modified particles. However, the cellular influences of pristine platinum nanoparticles are still unclear, although it has been reported that platinum nanoparticles induce oxidative stress. In this study, we investigated the cellular influences induced by pure pristine platinum nanoparticles. Platinum nanoparticles of 100% purity were dispersed in a cell culture medium and stable medium dispersion was obtained. The platinum nanoparticle medium dispersion was applied to two kinds of cultured cells, A549 and HaCaT cells, and the cellular influences were examined. Cell viability (MTT assay), cell proliferation (clonogenic assay), apoptosis induction (caspase-3 activity), intracellular ROS level (DCFH assay), and lipid peroxidation level (DPPP assay) were measured as markers of cellular influences. Transmission electron microscope observation showed cellular uptake of platinum nanoparticles. However, the platinum nanoparticles did not drive any markers. It is known that some metal oxide nanoparticles such as NiO and CuO show severe cytotoxicity via metal ion release. Compared with these toxic nanoparticles, the platinum nanoparticles used in this study did not release platinum ions into the culture media. These results suggest that the physically and chemically inactive cellular influences of platinum nanoparticles are small.
authors.affiliation: Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan, Kitakyushu, Fukuoka 807-8555, Japan. masa-horie@med.uoeh-u-ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kato
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Endoh
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Fujita
authors.forename: Keiko
authors.initials: K
authors.lastname: Nishio
authors.forename: Lilian Kaede
authors.initials: LK
authors.lastname: Komaba
authors.forename: Hiroko
authors.initials: H
authors.lastname: Fukui
authors.forename: Ayako
authors.initials: A
authors.lastname: Nakamura
authors.forename: Arisa
authors.initials: A
authors.lastname: Miyauchi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakazato
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kinugasa
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Morimoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry*; D015227:Lipid Peroxidation; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D010984:Platinum / Q000737:chemistry / Q000494:pharmacology*; D017671:Platinum Compounds / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D003470:Culture Media; D017671:Platinum Compounds; D017382:Reactive Oxygen Species; C030173:platinum chloride; D010984:Platinum