title: [Detection and clinical characteristics analysis of human bocavirus 1-3 in children for acute respiratory infection in Lanzhou area].
abstract: OBJECTIVE
  To study the clinical and molecular epidemiology characteristics of human Bocavirus 1-3 (HBoV1-3) in children for acute respiratory infection in Lanzhou area.
  
  
  METHODS
  Nasopharyngeal aspiration samples and throat swabs were collected from 524 children with ARTI at the First Hospital of Lanzhou University, Gansu Province, China, between December 2009 and November 2010. Nested PCR was employed to screening HBoV1-3, which amplified a 518-bp fragment of the partial NS1 gene. Furthermore, a standard reverse transcription-PCR was used to screen for other common respiratory viruses.
  
  
  RESULTS
  The overall frequency of HBoV was 8.2% (43/524), lining up behind human rhinovirus, RSV, parainfluenza virus 3. Thirty of the HBoV-postive children(69.8%) were co-infected with other respiratory viruses. The prevalence of HBoV1 in ALRTI was obviously higher than that in AURI. The 2 HBoV2 NS1 sequences shared 99% and 100% nucleotide sequence identity with HBoV2 strain CU47TH respectively. Two cases of HBoV2 postive children appears gastrointestinal symptoms. The one HBoV3 NS1 sequences shared 99% nucleotide sequence identity with HBoV3 isolate 46-BJ07.
  
  
  CONCLUSIONS
  The HBoV3 was detected at the first time in lanzhou area. HBoV1-3 infection exists in children with acute respiratory tract infections in Lanzhou region, HBoV1 were dominant. The mixed infection rate was higher.
authors.affiliation: Department of Pediatrics, First Hospital, Lanzhou 730000, China.
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Cao
authors.forename: Yu-ning
authors.initials: YN
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Zhi-ping
authors.initials: ZP
authors.lastname: Xie
authors.forename: Han-chun
authors.initials: HC
authors.lastname: Gao
authors.forename: Qiong-hua
authors.initials: QH
authors.lastname: Zhou
authors.forename: Xiao-qian
authors.initials: XQ
authors.lastname: Gao
authors.forename: Ya-ting
authors.initials: YT
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000208:Acute Disease; D002681:China; D005260:Female; D056661:Human bocavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D008297:Male; D010802:Phylogeny; D012141:Respiratory Tract Infections / Q000821:virology*