title: Performance of the New INNO-LiPA HPV extra to genotype human papillomavirus in cervical cell specimens.
abstract: OBJECTIVE
  It was our aim to compare the INNO-LiPA HPV Genotyping Extra (LiPA; Innogenetics) versus the Linear Array test (LA; Roche) in cervical samples.
  
  
  METHODS
  One hundred cervical samples were selected, obtained from a cancer prevention center. All samples were human papillomavirus (HPV) DNA positive by Hybrid Capture 2 HPV test and genotyped by LA HPV genotyping test. All data were analyzed using Cohen's κ tests. κ values ranged from poor, fair, moderate and good to very good agreement strength.
  
  
  RESULTS
  Detection of multiple infection and HPV genotypes per sample was higher by LA than by LiPA (61.5 vs. 52.1 and 2.2 vs. 1.7%, respectively). There were concordant results in 65 samples and compatible results in 33 samples. Only 2 samples were considered as discordant. In 21 samples, additional types were detected by LA, and in 13 samples, additional types were detected by LiPA. Analyzing the κ values, we have found very good agreement for 14 genotypes (6, 16, 26, 31, 33, 35, 45, 51, 52, 53, 58, 66, 68 and 70).
  
  
  CONCLUSIONS
  We considered that the new LiPA is highly comparable with other methods and suitable for clinical and epidemiological studies.
authors.affiliation: Clinical Microbiology Laboratory, Puerta del Mar University Hospital, University of Cadiz, Cádiz, Spain.
authors.forename: Fátima
authors.initials: F
authors.lastname: Galan-Sanchez
authors.forename: Maite
authors.initials: M
authors.lastname: Hernández-Menendez
authors.forename: María Angeles
authors.initials: MA
authors.lastname: De Los Rios Hernandez
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodriguez-Iglesias
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000821:virology; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000821:virology; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000235:genetics* / Q000821:virology
chemical_list: D004279:DNA, Viral