title: Performance evaluation of manual and automated (MagNA pure) nucleic acid isolation in HPV detection and genotyping using Roche Linear Array HPV Test.
abstract: Nucleic acids of human papillomavirus (HPV) isolated by manual extraction method (AmpliLute) and automated MagNA pure system were compared and evaluated with cytohistological findings in 253 women. The concordance level between AmpliLute and MagNA was very good 93.3% (κ = 0.864, P < .0001). Overall HPVpositivity detected by AmpliLute was 57.3% (30.4% as single and 27% as multiple infections) in contrast to MagNA 54.5% (32% and 23%, resp.). Discrepant results observed in 25 cases: 11 MagNA(-)/AmpliLute(+), 10 of which had positive histology; 5 MagNA(+)/AmpliLute(-) with negative histology; 8 MagNA(+)/AmpliLute(+): in 7 of which AmpliLute detected extra HPV genotypes and 1 MagNA(invalid)/AmpliLute(+) with positive histology. Both methods performed well when compared against cytological (area under curve (AUC) of AmpliLute 0.712 versus 0.672 of MagNA) and histological diagnoses (AUC of AmpliLute 0.935 versus 0.877 of MagNA), with AmpliLute showing a slightly predominance over MagNA. However, higher sensitivities, specificities, and positive/negative predictive values were obtained by AmpliLute.
authors.affiliation: Department of Diagnostic Cytopathology, University General Hospital Attikon, Athens, Greece.
authors.forename: Aikaterini
authors.initials: A
authors.lastname: Chranioti
authors.forename: Evangelia
authors.initials: E
authors.lastname: Aga
authors.forename: Niki
authors.initials: N
authors.lastname: Margari
authors.forename: Christine
authors.initials: C
authors.lastname: Kottaridi
authors.forename: Asimakis
authors.initials: A
authors.lastname: Pappas
authors.forename: Ioannis
authors.initials: I
authors.lastname: Panayiotides
authors.forename: Petros
authors.initials: P
authors.lastname: Karakitsos
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012372:ROC Curve; D011933:Reagent Kits, Diagnostic; D014773:Virology / Q000379:methods*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic