title: Systems biology and physical biology of clathrin-mediated endocytosis.
abstract: In this review, we describe the application of experimental data and modeling of intracellular endocytic trafficking mechanisms with a focus on the process of clathrin-mediated endocytosis. A detailed parts-list for the protein-protein interactions in clathrin-mediated endocytosis has been available for some time. However, recent experimental, theoretical, and computational tools have proved to be critical in establishing a sequence of events, cooperative dynamics, and energetics of the intracellular process. On the experimental front, total internal reflection fluorescence microscopy, photo-activated localization microscopy, and spinning-disk confocal microscopy have focused on assembly and patterning of endocytic proteins at the membrane, while on the theory front, minimal theoretical models for clathrin nucleation, biophysical models for membrane curvature and bending elasticity, as well as methods from computational structural and systems biology, have proved insightful in describing membrane topologies, curvature mechanisms, and energetics.
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 210 South 33rd Street, Philadelphia, PA 19104, USA.
authors.forename: Vyas
authors.initials: V
authors.lastname: Ramanan
authors.forename: Neeraj J
authors.initials: NJ
authors.lastname: Agrawal
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Sean
authors.initials: S
authors.lastname: Engles
authors.forename: Randall
authors.initials: R
authors.lastname: Toy
authors.forename: Ravi
authors.initials: R
authors.lastname: Radhakrishnan
mesh_terms: D000818:Animals; D016880:Anisotropy; D001703:Biophysics / Q000379:methods; D002966:Clathrin / Q000378:metabolism*; D004548:Elasticity; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008027:Light; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D025941:Protein Interaction Mapping; D012984:Software; D049490:Systems Biology
chemical_list: D002966:Clathrin