title: Effect of copper on levels of collagen and alkaline phosphatase activity from chondrocytes in newborn piglets in vitro.
abstract: The effects of different concentrations of copper on collagen content and alkaline phosphatase (AKP) activity from chondrocytes in newborn piglets were measured. Chondrocytes were cultured in media containing 15% fetal calf serum supplemented with 0, 15.6, 31.2, and 62.5 μmol/L copper in a 12-well culture plate. Collagen content and AKP activity from the chondrocyte extracellular matrix increased significantly in the culture media with 15.6, 31.2, and 62.5 μmol/L copper and was the highest at 31.2 μmol/L copper (P < 0.05). Thus, the results indicated that copper could promote AKP activity and collagen production by chondrocytes.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, 130062, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yuan
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Ai
authors.forename: Guoquan
authors.initials: G
authors.lastname: Sun
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Guowen
authors.initials: G
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D003300:Copper / Q000494:pharmacology*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D066298:In Vitro Techniques; D013268:Stimulation, Chemical; D013552:Swine
chemical_list: D003470:Culture Media; D003300:Copper; D003094:Collagen; D000469:Alkaline Phosphatase; D006909:Hydroxyproline