title: cDNA microarray analysis of the gene expression of murine leukemia RAW 264.7 cells after exposure to propofol.
abstract: Propofol (2,6-diisopropylphenol) is the most extensively used general anesthetic-sedative agent and it is employed in clinical patients. It has been shown that propofol exhibits anticancer activities. However, there is no available information to address propofol-induced cytotoxic effects and affected gene expressions on murine leukemia cells. Therefore, we investigated the effects of propofol on the levels of protein and gene expression, which are associated with apoptotic death in mouse leukemia RAW 264.7 cells in vitro. Results indicated that propofol induced cell morphological changes, cytotoxicity, and induction of apoptosis in RAW 264.7 cells in vitro. Western blot analysis demonstrated that propofol promoted Fas, cytochrome c, caspase-9 and -3 active form and Bax levels, but inhibited Bcl-xl protein level which led to cell apoptosis. Furthermore, cDNA microarray assay indicated that propofol significantly enhanced 5 gene expressions (Gm4884; Gm10883; Lce1c; Lrg1; and LOC100045878) and significantly suppressed 26 gene expressions (Gm10679; Zfp617; LOC621831; LOC621831; Gm5929; Snord116; Gm3994; LOC380994; Gm5592; LOC380994; Gm4638; LOC280487; Gm4638; Tex24; A530064D06Rik; BC094916; EG668725; Gm189; Hist2h3c2; Gm8020; Snord115; Gm3079; Olfr198; Tdh; Snord115; and Olfr1249). Based on these observations, propofol-altered apoptosis-related proteins might result from induction of apoptotic gene expression and inhibition of cell growth gene expression, which finally led to apoptosis in a mouse leukemia cell line (RAW 264.7) in vitro.
authors.affiliation: Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan.
authors.forename: Rick Sai-Chuen
authors.initials: RS
authors.lastname: Wu
authors.forename: Kuo-Ching
authors.initials: KC
authors.lastname: Liu
authors.forename: Nou-Ying
authors.initials: NY
authors.lastname: Tang
authors.forename: Hsiung-Kwang
authors.initials: HK
authors.lastname: Chung
authors.forename: Siu-Wan
authors.initials: SW
authors.lastname: Ip
authors.forename: Jai-Sing
authors.initials: JS
authors.lastname: Yang
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002843:Chromatin / Q000648:ultrastructure; D004354:Drug Screening Assays, Antitumor; D015870:Gene Expression; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D015742:Propofol / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D002843:Chromatin; D015742:Propofol