title: Induction of humoral and cellular immune responses against the HIV-1 envelope protein using γ-retroviral virus-like particles.
abstract: This study evaluates the immunogenicity of the HIV envelope protein (env) in mice presented either attached to γ-retroviral virus-like-particles (VLPs), associated with cell-derived microsomes or as solubilized recombinant protein (gp160). The magnitude and polyfunctionality of the cellular immune response was enhanced when delivering HIV env in the VLP or microsome form compared to recombinant gp160. Humoral responses measured by antibody titres were comparable across the groups and low levels of antibody neutralization were observed. Lastly, we identified stronger IgG2a class switching in the two particle-delivered antigen vaccinations modalities compared to recombinant gp160.
authors.affiliation: Department of Infectious Diseases, Aarhus University Hospital, Skejby, DK-8200 Aarhus N, Denmark.
authors.forename: Tea
authors.initials: T
authors.lastname: Kirkegaard
authors.forename: Adam
authors.initials: A
authors.lastname: Wheatley
authors.forename: Jesper
authors.initials: J
authors.lastname: Melchjorsen
authors.forename: Shervin
authors.initials: S
authors.lastname: Bahrami
authors.forename: Finn S
authors.initials: FS
authors.lastname: Pedersen
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Center
authors.forename: Damian F J
authors.initials: DF
authors.lastname: Purcell
authors.forename: Lars
authors.initials: L
authors.lastname: Ostergaard
authors.forename: Mogens
authors.initials: M
authors.lastname: Duch
authors.forename: Martin
authors.initials: M
authors.lastname: Tolstrup
mesh_terms: D000818:Animals; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D019302:HIV Envelope Protein gp160 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D022701:Virosomes / Q000276:immunology
chemical_list: D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D007074:Immunoglobulin G; D022701:Virosomes