title: [Prokaryotic expression and polyclonal antibody preparation of human Nodal mature peptide].
abstract: OBJECTIVE
  To express the fusion protein of human Nodal mature peptide in prokaryocytes and to prepare mouse anti-Nodal polyclonal antibody.
  
  
  METHODS
  The expression vector pReceiver-hNodal was constructed and transformed into BL21(DE3). His(6);-hNodal fusion protein was induced by IPTG and separated on SDS-PAGE and the recovered fusion protein was used to immune BALB/c to produce polyclonal antibody. The titer of the polyclonal antibody was detected by indirect ELISA and its specificity was analyzed byWestern blot and immunochemical staining.
  
  
  RESULTS
  The expected molecular weight of fusion protein around 15 000 was highly expressed in E.coli and specific antibody was obtained.
  
  
  CONCLUSIONS
  The successful preparation of anti-Nodal mature peptide polyclonal antibody will lay the basis for further study of the biological function of Nodal on tumor progress.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Li
authors.forename: Guan-min
authors.initials: GM
authors.lastname: Jiang
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Jun
authors.initials: J
authors.lastname: DU
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D055457:Nodal Protein / Q000235:genetics* / Q000276:immunology*; D010455:Peptides / Q000235:genetics* / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000906:Antibodies; C526007:NODAL protein, human; D055457:Nodal Protein; D010455:Peptides; D011993:Recombinant Fusion Proteins