title: Effect of tobacco compounds on gene expression profiles in human epithelial cells.
abstract: This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.
authors.affiliation: Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Sung-Hwa
authors.initials: SH
authors.lastname: Sohn
authors.forename: Jaebum
authors.initials: J
authors.lastname: Lee
authors.forename: Ki-Nam
authors.initials: KN
authors.lastname: Kim
authors.forename: In Kyoung
authors.initials: IK
authors.lastname: Kim
authors.forename: Meyoung-Kon
authors.initials: MK
authors.lastname: Kim