title: Aluminum nanoparticle exposure in L1 larvae results in more severe lethality toxicity than in L4 larvae or young adults by strengthening the formation of stress response and intestinal lipofuscin accumulation in nematodes.
abstract: Toxicity of Al(2)O(3)-NPs, as compared to that of Al(2)O(3), to L1-larval, L4-larval or young adult nematodes was evaluated. When exposure was performed at L1-larval stage, the significant increases of lethality, stress response, and intestinal lipofuscin autofluorescence were observed in 6.3-203.9 mg/L of Al(2)O(3)-NPs exposed nematodes. In contrast, when exposure was performed at L4-larval or young adult stage, the significant increases of lethality and intestinal lipofuscin autofluorescence were observed in 12.7-203.9 mg/L of Al(2)O(3)-NPs exposed nematodes, and the significant inductions of stress response were detected in 25.5-203.9 mg/L of Al(2)O(3)-NPs exposed nematodes. Moreover, the lethality was significantly correlated with the stress response and the intestinal lipofuscin autofluorescence in Al(2)O(3)-NPs exposed nematodes. These data imply that Al(2)O(3)-NPs exposure in L1 larvae causes more severe lethality toxicity than in L4 larvae or young adults by strengthening the formation of stress response and intestinal lipofuscin accumulation in nematodes.
authors.affiliation: Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210009, China.
authors.forename: Si
authors.initials: S
authors.lastname: Wu
authors.forename: Jianhong
authors.initials: J
authors.lastname: Lu
authors.forename: Qi
authors.initials: Q
authors.lastname: Rui
authors.forename: Shunhui
authors.initials: S
authors.lastname: Yu
authors.forename: Ting
authors.initials: T
authors.lastname: Cai
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000502:physiology; D000537:Aluminum Oxide / Q000633:toxicity*; D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000254:growth & development* / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects / Q000254:growth & development; D007814:Larva; D016014:Linear Models; D008062:Lipofuscin / Q000378:metabolism*; D053758:Nanoparticles / Q000633:toxicity*; D013045:Species Specificity; D013534:Survival
chemical_list: D029742:Caenorhabditis elegans Proteins; D006360:Heat-Shock Proteins; D007202:Indicators and Reagents; D008062:Lipofuscin; C108431:hsp-16.2 protein, C elegans; D000537:Aluminum Oxide