title: Molecular mass spectrometric identification of superoxide dismutase in the liver of mice Mus musculus and Mus spretus using a metallomics analytical approach.
abstract: This paper reports the identification and quantification of superoxide dismutase in the liver of Mus musculus and Mus spretus mice using a metallomics analytical approach. The approach consisted of using orthogonal chromatographic systems coupled to ICP-MS and UV detectors. Size-exclusion fractionation of the cytosolic extracts was followed by anion-exchange chromatographic separation of Cu- and Zn-containing species. After purification then tryptic digestion, Cu- and Zn-containing superoxide dismutase was identified by nESI-QqTOF. The MS-MS spectra of doubly charged peptides, with the Mascot searching engine, were used to obtain the sequence of the protein.
authors.affiliation: Department of Chemistry and Material Science, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain.
authors.forename: M
authors.initials: M
authors.lastname: González-Fernández
authors.forename: T
authors.initials: T
authors.lastname: García-Barrera
authors.forename: J L
authors.initials: JL
authors.lastname: Gómez-Ariza
mesh_terms: D000465:Algorithms; D000818:Animals; D002457:Cell Extracts / Q000737:chemistry*; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D008099:Liver / Q000737:chemistry*; D051379:Mice; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods*; D013482:Superoxide Dismutase / Q000032:analysis* / Q000145:classification; D053719:Tandem Mass Spectrometry; D014357:Trypsin / Q000378:metabolism
chemical_list: D002457:Cell Extracts; D013482:Superoxide Dismutase; D014357:Trypsin