title: [Interaction of diphtheria toxin B subunit with sensitive and insensitive mammalian cells].
abstract: The recombinant fluorescent derivative of diphtheria toxin (EGFP-SbB) obtained by the replacement of toxin A subunit by enhanced green fluorescent protein (EGFP) has been used for visualization of the interaction of diphtheria toxin (DT) with sensitive and insensitive cells. It was shown that EGFP-SbB could interact with cell surface of both toxin-sensitive monkey cells (Vero cell line) and toxin-resistant mouse cells (3T3 cell line). The affinity of this protein for receptors of Vero cells was three times higher as compared with 3T3 cells. It was demonstrated that fluorescent derivate was able to interact with receptors of both cell lines and to internalize into these cells. Internalization of EGFP-SbB into the cells was inhibited by endocytosis inhibitor phenyl arsine oxide. We suppose that diverse sensitivity to DT of monkey and mouse cells can be explained not only by differences in their receptor affinity for DT but also by the processes that occur after internalization of the toxin into the cells.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Labyntsev
authors.forename: N V
authors.initials: NV
authors.lastname: Korotkevich
authors.forename: A A
authors.initials: AA
authors.lastname: Kaberniuk
authors.forename: S I
authors.initials: SI
authors.lastname: Romaniuk
authors.forename: D V
authors.initials: DV
authors.lastname: Kolybo
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004167:Diphtheria Toxin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli; D005434:Flow Cytometry; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D066257:Heparin-binding EGF-like Growth Factor; D058507:Host Specificity; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D007700:Kinetics; D008322:Mammals; D051379:Mice; D018613:Microscopy, Confocal; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D001152:Arsenicals; D004167:Diphtheria Toxin; D004791:Enzyme Inhibitors; C587884:Hbegf protein, mouse; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; C029341:oxophenylarsine; D049452:Green Fluorescent Proteins