title: A novel flow cytometry-based technique to measure adult neurogenesis in the brain.
abstract: The stimulation of neurogenesis is an exciting novel therapeutic option for diseases of the central nervous system, ranging from depression to neurodegeneration. One major bottleneck in screening approaches for neurogenesis-inducing compounds is the very demanding in vivo quantification of newborn neurons based on stereological techniques. To effectively develop compounds in this area, novel fast and reliable techniques for quantification of in vivo neurogenesis are needed. In this study, we introduce a flow cytometry-based method for quantifying newly generated neurons in the brain based on the counting of cell nuclei from dissected brain regions. Important steps involve density sedimentation of the cell nuclei, and staining for the proliferation marker bromodeoxy uridine and nuclear cell type markers such as NeuN. We demonstrate the ability of the technique to detect increased neurogenesis in the hippocampus of animals which underwent physical exercise and received fluoxetine treatment.
authors.affiliation: SYGNIS Bioscience, Im Neuenheimer Feld 515, Heidelberg, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Spoelgen
authors.forename: Annette
authors.initials: A
authors.lastname: Meyer
authors.forename: Anja
authors.initials: A
authors.lastname: Moraru
authors.forename: Friederike
authors.initials: F
authors.lastname: Kirsch
authors.forename: Angela
authors.initials: A
authors.lastname: Vogt-Eisele
authors.forename: Christian
authors.initials: C
authors.lastname: Plaas
authors.forename: Claudia
authors.initials: C
authors.lastname: Pitzer
authors.forename: Armin
authors.initials: A
authors.lastname: Schneider
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001921:Brain / Q000187:drug effects / Q000502:physiology*; D001973:Bromodeoxyuridine; D002452:Cell Count; D002467:Cell Nucleus / Q000502:physiology; D002499:Centrifugation, Density Gradient; D004268:DNA-Binding Proteins; D005434:Flow Cytometry; D005473:Fluoxetine / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000494:pharmacology
chemical_list: D000963:Antimetabolites; D001973:Bromodeoxyuridine; D004268:DNA-Binding Proteins; D005473:Fluoxetine; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D017367:Selective Serotonin Reuptake Inhibitors; C492380:NeuN protein, mouse