title: Different IgG-subclass distributions after whole-cell and acellular pertussis infant primary vaccinations in healthy and pertussis infected children.
abstract: The distribution of IgG-subclasses provides insight in the immunological mechanisms of protection against whooping cough. We investigated the effect of Dutch whole-cell pertussis and acellular pertussis vaccines administered in infancy on the IgG-subclass distributions in healthy children aged 12 months, 4 years and 9 years as well as in children who have been infected with Bordetella pertussis. A fluorescent bead-based multiplex immunoassay was used for the measurement of IgG1, IgG2, IgG3 and IgG4 responses against pertussis toxin, filamentous heamagglutinin and pertactin. Although IgG1 was the predominant subclass for all pertussis antigens in both healthy and infected children, elevated IgG4 levels were only present in children who had received repeated number of acellular pertussis vaccinations. IgG2 and IgG3 antibodies did not contribute to the IgG response. No differences in IgG-subclasses between healthy vaccinated or infected children were found. The pertussis vaccine used for priming seems to determine the IgG-subclass composition elicited after a secondary antibody response either induced by pertussis vaccination or infection. The pronounced anti-pertussis IgG4 response might reflect the Th2-skewing of the immune response after aP vaccination.
authors.affiliation: Centre for Infectious Disease and Control (Cib), National Institute for Public Health and the Environment, Bilthoven, The Netherlands. Lotte.Hendrikx@rivm.nl
authors.forename: Lotte H
authors.initials: LH
authors.lastname: Hendrikx
authors.forename: Rose-Minke
authors.initials: RM
authors.lastname: Schure
authors.forename: Kemal
authors.initials: K
authors.lastname: Oztürk
authors.forename: Lia G H
authors.initials: LG
authors.lastname: de Rond
authors.forename: S C
authors.initials: SC
authors.lastname: de Greeff
authors.forename: Elisabeth A M
authors.initials: EA
authors.lastname: Sanders
authors.forename: Guy A M
authors.initials: GA
authors.lastname: Berbers
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Buisman
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000293:Adolescent; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology*; D007223:Infant; D037342:Pertussis Toxin / Q000276:immunology; D010567:Pertussis Vaccine / Q000008:administration & dosage* / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology* / Q000517:prevention & control*
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D007073:Immunoglobulin E; C068929:pertactin; D037342:Pertussis Toxin