title: Effect comparison of different formulation of Dang-Gui-Bu-Xu-Tang on myelosuppression mouse.
abstract: OBJECTIVE
  To compare effect of different formulation of Dang-Gui-Bu-Xu-Tang(DGBXT) on myelosuppression mouse.
  
  
  METHODS
  HPLC was used to measure active ingredients of two DGBXT formulations. Hemopoesic function of bone marrow was measured by hemopoietic progenitor cell culture and peipheral blood count. And hemopoietic factors in bone marrow were tested by ELISA.
  
  
  RESULTS
  The content level of astragaloside A in granule formulation was higher than that in decoction and the content ratio of active ingredient was close to 5 : 1. Two DGBXD formulations could significantly improve amount of peripheral blood cells, and bone marrow cells of bone marrow suppression mice (P<0.05). DGBXD granule formulation significantly increased the colony quantity of all progenitor cell lines and amount of G(2)/M and S phase cells (P<0.05). It also significantly decreased amount of G(0)/G(1) phase cells in the bone marrow and was more effective (P<0.05).
  
  
  CONCLUSIONS
  DGBXT decoction and the granule formulation all can improve the hematopoietic function of bone marrow suppression mouse. They can improve quantities of peripheral blood and nucleated bone marrow cells, and yield of the hematopoietic stem/progenitor cells in vitro colony; balance the expression of cytokine (EPO, TPO and GM-CSF) in bone marrow microenvirement. They can also facilitate hematopoietic stem/progenitor cells to enter the cell cycle. And the effect of granule formulation is more satisfactory.
authors.affiliation: General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Suchun
authors.initials: S
authors.lastname: Yan
authors.forename: Yiqiang
authors.initials: Y
authors.lastname: Xie
authors.forename: Bide
authors.initials: B
authors.lastname: Zhu
authors.forename: Yingguang
authors.initials: Y
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D003373:Coumaric Acids / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D004921:Erythropoietin / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D016867:Immunocompromised Host / Q000187:drug effects* / Q000528:radiation effects; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012503:Saponins / Q000032:analysis; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014315:Triterpenes / Q000032:analysis; D014916:Whole-Body Irradiation
chemical_list: D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D012503:Saponins; D014315:Triterpenes; C477279:danggui buxue decoction; D004921:Erythropoietin; C052064:astragaloside A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C004999:ferulic acid