title: [Isotypes of anti-pertussis antibodies in pertussis patients at different stages of the disease].
abstract: OBJECTIVE
  Evaluation of anti-pertussis antibodies in pertussis patients at different stages after the onset of the disease.
  
  
  METHODS
  Levels of IgG, IgG1, IgG2, IgG3, IgG4, IgA and IgM antibodies against the antigen complex of pertussis were evaluated by enzyme immunoassay (EIA). Sera samples were analyzed from 208 pertussis patients examined from week 1 to 10 after the onset of the disease.
  
  
  RESULTS
  51%, 82% and 86% pertussis patients, and 67%, 72% and 78% patients examined from week 1 to 3 after the onset of the disease had increased levels of IgM, IgA and IgG antibodies respectively. 85%, 70%, 74% and 68% pertussis patients, and 76%, 57%, 87%, 57% patients examined from week 1 to 3 after the onset of the disease had increased IgG1, IgG2, IgG3 and IgG4 levels respectively. 92% of all examined pertussis patients and 83% of patients examined from week 1 to 3 after the onset of the disease had an overall increase of anti-pertussis antibody levels. Increased level of IgM antibodies was detected predominately from week 1 to 5 after the onset of the disease. Most of the patients examined from week 3 to 10 after the onset of the disease had increased levels of IgA, IgG, IgG1, IgG2 and IgG4 antibodies, and IgG3 antibody level was increased predominately in patients examined from week 2 to 6 after the onset of the disease.
  
  
  CONCLUSIONS
  Serological indicators of pertussis measured by EIA were observed in 83% of the patients examined at the early stages after the onset of the disease. Simultaneous measurement of IgA, IgG and IgM antibody levels is the most effective approach for serological diagnostics of pertussis due anti-pertussis antibodies isotype composition heterogeneity at different stages after the onset of the disease. Increased levels of IgM and IgG3 antibodies are serologic indicators of the acute phase of pertussis infection.
authors.forename: E M
authors.initials: EM
authors.lastname: Zaĭtsev
authors.forename: L I
authors.initials: LI
authors.lastname: Krasnoproshina
authors.forename: I K
authors.initials: IK
authors.lastname: Mazurova
authors.forename: M S
authors.initials: MS
authors.lastname: Petrova
authors.forename: O P
authors.initials: OP
authors.lastname: Popova
authors.forename: N S
authors.initials: NS
authors.lastname: Zakharova
mesh_terms: D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007132:Immunoglobulin Isotypes / Q000097:blood*; D007223:Infant; D008297:Male; D010567:Pertussis Vaccine / Q000276:immunology; D014917:Whooping Cough / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007132:Immunoglobulin Isotypes; D010567:Pertussis Vaccine