title: Identification of a Tc1-like transposon integration site in the genome of the flounder (Platichthys flesus): a novel use of an inverse PCR method.
abstract: The inverse PCR method has been developed and applied employed for the identification of the integration sites of the Tc1-like transposons in the genome of the flounder, Platichthys flesus. One Tc1-like insertion instance was recognized and characterized, demonstrating an efficiency of the method for determining of transposon integration sites. The similarity of the sequence flanking transposon (SFT) to reverse transcriptase sequences (RVT) was demonstrated. It is likely that the insertion took place within currently degenerated LINE (long interspersed nuclear elements) retrotransposon.
authors.affiliation: Department of Genetics and Marine Biotechnology, Institute of Oceanology, Polish Academy of Sciences, Powstancow Warszawy, Sopot, Poland. anita@iopan.gda.pl
authors.forename: Anita
authors.initials: A
authors.lastname: Poćwierz-Kotus
authors.forename: Artur
authors.initials: A
authors.lastname: Burzyński
authors.forename: Roman
authors.initials: R
authors.lastname: Wenne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005432:Flounder / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020079:Terminal Repeat Sequences / Q000235:genetics; D019895:Transposases / Q000235:genetics
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C081454:Tc1 transposase; D019895:Transposases