title: Circulating monocytes are not a major reservoir of HIV-1 in elite suppressors.
abstract: Circulating HIV-1-infected monocytes have been identified in patients on highly active antiretroviral therapy and may represent an important barrier to viral eradication. The nature of these cells in HIV-1-infected patients who maintain undetectable viral loads and preserved CD4(+) T cell counts without antiretroviral therapy (known as elite controllers or elite suppressors [ES]) is unknown. We describe here infrequent recovery of proviral HIV-1 DNA from circulating monocytes relative to CD4(+) T cells in ES, despite permissiveness of these cells to HIV-1 viral entry ex vivo. Thus, monocytes do not appear to be a major reservoir of HIV-1 in ES.
authors.affiliation: Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, MD 21205, USA.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Spivak
authors.forename: Maria
authors.initials: M
authors.lastname: Salgado
authors.forename: S Alireza
authors.initials: SA
authors.lastname: Rabi
authors.forename: Karen A
authors.initials: KA
authors.lastname: O'Connell
authors.forename: Joel N
authors.initials: JN
authors.lastname: Blankson
mesh_terms: D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015658:HIV Infections / Q000276:immunology* / Q000821:virology*; D020276:HIV Long-Term Survivors; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009000:Monocytes / Q000821:virology*; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral