title: Oriental HLA-A*11:90 detected in a Taiwanese cord blood sample and the haplotype in association with A*11:90 allele.
abstract: We report here an HLA-A allele, A*11:90, found in a Taiwanese cord blood sample using DNA sequence-based typing (SBT) protocol after observing an anomalous reaction pattern in a sequence-specific oligonucleotide (SSO) typing exercise. The sequence of A*11:90 is identical to A*11:01:01, the most predominant A*11 variant in Taiwanese, in exon 2 but differs from A*11:01:01 in exon 3 by two nucleotide substitutions at codon 163 (c.487C>G and c.488G>A), resulting R163E. In comparison with the sequence of A*11:02:01, the second most predominant subtype of A*11 in Taiwanese A*11:90 has one nucleotide difference at codon 19 (c.55A>G) in exon 2 resulting K19E and two nucleotides variations at codon 163 (c.487C>G and c.488G>A) in exon 3 resulting R163E. HLA-A*11:90-B*40:02-DRB1*11:01 is the deduced probable HLA haplotype in association with A*11:90. The generation of A*11:90 is thought to involve a DNA recombination event between alleles A*11:01:01 and A*80:01 where A*80:01 donated a fragment of the DNA sequence (from n.t. 487 to n.t. 497) to the recipient sequence of A*11:01:01.
authors.affiliation: Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
authors.forename: K L
authors.initials: KL
authors.lastname: Yang
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
authors.forename: S
authors.initials: S
authors.lastname: Jiang
authors.forename: H M
authors.initials: HM
authors.lastname: Chiu
authors.forename: S
authors.initials: S
authors.lastname: Lin
authors.forename: Y C
authors.initials: YC
authors.lastname: Chen
authors.forename: Y F
authors.initials: YF
authors.lastname: Jian
authors.forename: S Y
authors.initials: SY
authors.lastname: Yang
authors.forename: Y B
authors.initials: YB
authors.lastname: Yang
authors.forename: S B
authors.initials: SB
authors.lastname: Chen
authors.forename: M H
authors.initials: MH
authors.lastname: Shyr
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D005312:Fetal Blood / Q000276:immunology* / Q000378:metabolism; D056726:Genetic Association Studies; D015234:HLA-A Antigens / Q000737:chemistry / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013624:Taiwan
chemical_list: D015234:HLA-A Antigens