title: Using matrix-assisted laser desorption ionization-time of flight mass spectrometry to detect carbapenem resistance within 1 to 2.5 hours.
abstract: In recent years, the percentage of carbapenem-resistant bacteria has increased at an alarming pace and become a major threat for patient survival. Carbapenemase-induced carbapenem resistance can be confirmed through the detection of carbapenem degradation using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). This method works for strains carrying NDM-1, VIM-1, VIM-2, KPC-2, and different IMP enzymes.
authors.affiliation: Department for Infectious Diseases, Microbiology and Hygiene, University of Heidelberg, Im Neuenheimer Feld 324, D-69115 Heidelberg, Germany. irene.burckhardt@med.uni-heidelberg.de
authors.forename: Irene
authors.initials: I
authors.lastname: Burckhardt
authors.forename: Stefan
authors.initials: S
authors.lastname: Zimmermann
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001419:Bacteria / Q000737:chemistry* / Q000187:drug effects*; D015780:Carbapenems / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems