title: Prevalence of cytomegalovirus infection and its role in total immunoglobulin pattern in Iranian patients with different subtypes of multiple sclerosis.
abstract: Multiple sclerosis (MS) is the most common autoimmune disease characterized by multifocal areas of inflammatory demyelination within the central nervous system. Cytomegalovirus (CMV) has a complex pathobiology and in most cases is simply asymptomatic. There is some recent controversy over the role of CMV in the pathology of MS. The aim of this study was to evaluate active CMV infection and its effect on the humoral immunity in patients with MS. Serum, plasma, peripheral blood mononuclear cells (PBMCs), saliva and urine collected from MS patients (n=78) and healthy subjects (n=123) were screened for the presence of anti-CMV antibodies and CMV-DNA by nephelometric and PCR methods. Concentrations of total antibodies in MS subtypes were measured using both nephelometric and enzyme linked fluorescent assay (ELFA) techniques. The results extend the observation of an increased frequency of CMV-DNA in patients, in contrast with controls (p<0.001). Furthermore, systemic CMV infections were found in 25.5% of patients and only 3.2% of controls (p<0.001). There was significant difference in the titers of anti-CMV IgG and total IgE in patient and controls (P<0.001). These results support the hypothesis that CMV may contribute to MS thought to establish systemic infection process and induce immune response.
authors.affiliation: Department of Biology, Faculty of Science, Zabol University, Zabol, Iran. n.sanadgol@uoz.ac.ir
authors.forename: Nima
authors.initials: N
authors.lastname: Sanadgol
authors.forename: Nourollah
authors.initials: N
authors.lastname: Ramroodi
authors.forename: Ghasem Ali
authors.initials: GA
authors.lastname: Ahmadi
authors.forename: Majid
authors.initials: M
authors.lastname: Komijani
authors.forename: Ali
authors.initials: A
authors.lastname: Moghtaderi
authors.forename: Majid
authors.initials: M
authors.lastname: Bouzari
authors.forename: Marzieh
authors.initials: M
authors.lastname: Rezaei
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Kardi
authors.forename: Soroush
authors.initials: S
authors.lastname: Dabiri
authors.forename: Malek
authors.initials: M
authors.lastname: Moradi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Sanadgol
mesh_terms: D000293:Adolescent; D000328:Adult; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007136:Immunoglobulins / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000453:epidemiology / Q000276:immunology* / Q000821:virology*; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D007075:Immunoglobulin M; D007136:Immunoglobulins; D007073:Immunoglobulin E