title: The activity of class I, II, III and IV alcohol dehydrogenase isoenzymes and aldehyde dehydrogenase in cervical cancer.
abstract: OBJECTIVE
  The aim of this study was to compare the metabolism of cervical cancer cells and normal cervical cells by measurement of ADH isoenzymes and ALDH activities.
  
  
  METHODS
  The study material consisted of 40 cancerous cervical tissues. Class III, IV of ADH and total ADH activity was measured by the photometric method and class I, II ADH and ALDH activity by the fluorometric method with class-specific fluorogenic substrates.
  
  
  RESULTS
  The activity of the class I ADH isoenzyme and the total ADH were significantly higher in cervical cancer as compared to healthy tissues. Class I of ADH and total ADH activity are significantly higher in every stage of cancer as compared to the control. There are no significant differences between planoepitheliale and adenocarcinoma.
  
  
  CONCLUSIONS
  The increased activity of total ADH in cervical cancer may be the cause of some metabolic disorders in cancer cells, which may intensify carcinogenesis in this organ.
authors.affiliation: Department of Biochemical Diagnostics, Medical University, Bialystok, Poland. orywalk@umwb.edu.pl
authors.forename: Karolina
authors.initials: K
authors.lastname: Orywal
authors.forename: Wojciech
authors.initials: W
authors.lastname: Jelski
authors.forename: Michał
authors.initials: M
authors.lastname: Zdrodowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Szmitkowski
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology; D000328:Adult; D000368:Aged; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008875:Middle Aged; D009367:Neoplasm Staging; D012016:Reference Values; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology; D055815:Young Adult
chemical_list: D007527:Isoenzymes; D000426:Alcohol Dehydrogenase; C105552:alcohol dehydrogenase IV; C020145:formaldehyde dehydrogenase (glutathione); D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase