title: Antioxidative effects of cherry leaves extract on tert-butyl hydroperoxide-mediated cytotoxicity through regulation of thioredoxin-2 protein expression levels.
abstract: Components of cherry trees have been used as traditional herbal remedies for various diseases. These components are known to possess antioxidative effects. However, the mechanisms underlying cherry tree component-mediated antioxidative effects remain largely unknown. This study focused on cherry leaves extract (CLE) and examined the mechanism underlying the effect of CLE on tert-butyl hydroperoxide (t-BOOH)-induced melanocytic cell death with DNA damage. Interestingly, CLE prevented t-BOOH-induced cell death with reduction in DNA damage, p38 kinase activation, and reactive oxygen species (ROS) production. CLE-mediated suppression of cell death with reduction of DNA damage, p38 kinase activity and ROS production was prevented by a thioredoxin (Trx) system inhibitor but not by a glutathione (GSH) system inhibitor. Finally, data showed that CLE prevented t-BOOH-induced reduction of Trx2 but not Trx1 and Trx reductases (TrxR1 and TrxR2) protein expression. Thus, our results suggest that CLE prevents t-BOOH-induced reduction in Trx2 expression, promotion of ROS production, activation of p38 kinase, and increase in DNA damage and that it protects against cell death.
authors.affiliation: Units of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai-shi, Aichi, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Taguchi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Uemura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sakura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Hara
authors.forename: Masanao
authors.initials: M
authors.lastname: Niwa
authors.forename: Machiko
authors.initials: M
authors.lastname: Iida
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yanagishita
authors.forename: Daisuke
authors.initials: D
authors.lastname: Watanabe
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005978:Glutathione / Q000037:antagonists & inhibitors; D006801:Humans; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D027861:Prunus / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013879:Thioredoxins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity
chemical_list: D000975:Antioxidants; D024101:Mitochondrial Proteins; D016877:Oxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C467002:TXN2 protein, human; D013879:Thioredoxins; D020122:tert-Butylhydroperoxide; D048051:p38 Mitogen-Activated Protein Kinases; D005978:Glutathione