title: DNA damage induction in human cells exposed to vanadium oxides in vitro.
abstract: Vanadium and vanadium salts cause genotoxicity and elicit variable biological effects depending on several factors. In the present study, we analyzed and compared the DNA damage and repair processes induced by vanadium in three oxidation states. We used human blood leukocytes in vitro and in a single cell gel electrophoresis assay at two pH values. We observed that vanadium(III) trioxide and vanadium(V) pentoxide produced DNA single-strand breaks at all of the concentrations (1, 2, 4, or 8 μg/ml) and treatment times (2, 4, or 6 h) tested. Vanadium(IV) tetraoxide treatment significantly increased DNA damage at all concentrations for 4 or 6 h of treatment but not for 2 h of treatment. The DNA repair kinetics indicated that most of the cells exposed to vanadium III and V for 4 h recovered within the repair incubation time of 90 min; however, those exposed to vanadium(IV) repaired their DNA within 120 min. The data at pH 9 indicated that vanadium(IV) tetraoxide induced DNA double-strand breaks. Our results show that the genotoxic effect of vanadium can be produced by any of its three oxidation states. However, vanadium(IV) induces double-strand breaks, and it is known that these lesions are linked with forming structural chromosomal aberrations.
authors.affiliation: Unidad de Investigación en Genética y Toxicología Ambiental (UNIGEN), Laboratorio L5-PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza, Campus II, UNAM, A.P. 9-020, C.P. 15000, México, D.F., Mexico.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Rodríguez-Mercado
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Mateos-Nava
authors.forename: Mario A
authors.initials: MA
authors.lastname: Altamirano-Lozano
mesh_terms: D000328:Adult; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000187:drug effects; D053960:DNA Breaks; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010087:Oxides / Q000633:toxicity*; D017968:Vanadium Compounds / Q000633:toxicity*
chemical_list: D004277:DNA, Single-Stranded; D004785:Environmental Pollutants; D010087:Oxides; D017968:Vanadium Compounds; D004247:DNA