title: CODA-RET reveals functional selectivity as a result of GPCR heteromerization.
abstract: Here we present a new method that combines protein complementation with resonance energy transfer to study conformational changes in response to activation of a defined G protein-coupled receptor heteromer, and we apply the approach to the putative dopamine D1-D2 receptor heteromer. Remarkably, the potency of the D2 dopamine receptor (D2R) agonist R-(-)-10,11-dihydroxy-N-n-propylnoraporphine (NPA) to change the Gα(i) conformation via the D2R protomer in the D1-D2 heteromer was enhanced ten-fold relative to its potency in the D2R homomer. In contrast, the potencies of the D2R agonists dopamine and quinpirole were the same in the homomer and heteromer. Thus, we have uncovered a molecular mechanism for functional selectivity in which a drug acts differently at a G protein-coupled receptor (GPCR) protomer depending on the identity of the second protomer participating in the formation of the signaling unit--opening the door to enhancing pharmacological specificity by targeting differences between homomeric and heteromeric signaling.
authors.affiliation: Center for Molecular Recognition and Departments of Psychiatry and Pharmacology, College of Physicians and Surgeons, Columbia University, New York, New York, USA.
authors.forename: Eneko
authors.initials: E
authors.lastname: Urizar
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yano
authors.forename: Rachel
authors.initials: R
authors.lastname: Kolster
authors.forename: Céline
authors.initials: C
authors.lastname: Galés
authors.forename: Nevin
authors.initials: N
authors.lastname: Lambert
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Javitch
mesh_terms: D001058:Apomorphine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D004298:Dopamine / Q000737:chemistry / Q000494:pharmacology; D018491:Dopamine Agonists / Q000737:chemistry / Q000494:pharmacology; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006801:Humans; D011487:Protein Conformation; D055503:Protein Multimerization; D019257:Quinpirole / Q000737:chemistry / Q000494:pharmacology; D017447:Receptors, Dopamine D1 / Q000819:agonists / Q000737:chemistry*; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000737:chemistry*
chemical_list: C425777:10,11-dihydroxy-N-n-propylnorapomorphine; D018491:Dopamine Agonists; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D019257:Quinpirole; D001058:Apomorphine; D004298:Dopamine