title: Grafting as a method for studying development in the filamentous fungus Podospora anserina.
abstract: While grafting and transplant experiments have extensively been used to study development in animals and plants, they have seldom been employed to study fungal development. Here, grafting is used to study the interplay between mycelium and multicellular fruiting bodies during maturation in the model ascomycete Podospora anserina. Data indicate that grafts need a competent mycelium to continue their ripening. Vegetative incompatibility does not prevent transplanted fructifications to undergo development. Grafting onto mutant mycelia confirmed a previous model stating that the NADPH oxidase PaNox1 is required in the developing fruiting bodies, while the MAP kinase cascade PaMpk1 is required in the mycelium. Data also show that the IDC1 protein is required not only in the developing fruiting bodies but also in the mycelium, likely because of its role in anastomosis. Finally, entry inside the grafted fruiting bodies of a ribosomal protein tagged with GFP could be detected, suggesting that cellular components are imported from the underlying mycelium during maturation.
authors.affiliation: Univ Paris-Sud, Institut de Génétique et Microbiologie, CNRS UMR 8621, 91405 Orsay Cedex, France. philippe.silar@igmors.u-psud.fr
authors.forename: Philippe
authors.initials: P
authors.lastname: Silar
mesh_terms: D048690:Fruiting Bodies, Fungal / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D008954:Models, Biological; D025282:Mycelium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D009172:Mycology / Q000379:methods*; D048468:Podospora / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism