title: Endothelial dysfunction after exposure to cobalt chloride enhanced vascular contractility.
abstract: Brief exposure to cobalt chloride augmented vascular contractility. We hypothesized that endothelial dysfunction plays a role in the augmentation of aortic contractility, after brief exposure to cobalt chloride. Rat aortic ring preparations were mounted in organ baths, exposed to cobalt chloride (0.3-300μmol/L) for 30min, and then subjected to contractile agents or relaxants 1 and 5h after the end of exposure. Presence of cobalt chloride did not affect the contractile response to phenylephrine. Brief exposure to cobalt chloride, however, even at 5h after the end of exposure, not only augmented contractile responses to KCl or phenylephrine but also attenuated the relaxant response to acetylcholine. The mechanical denudation of endothelium or inhibition of endothelial nitric oxide synthase with 100μmol/L N(ω)-nitro-l-arginine methyl ester abolished the augmentation of contractile responses. Pre-treatment with 150units/mL of superoxide dismutase also abrogated the augmented contractile responses. Brief exposure to cobalt chloride did not affect the contractile response to phorbol dibutyrate in the presence or absence of calcium, or the expression of HSP70. In conclusion, endothelial dysfunction plays an important role in the augmentation of aortic contractility, after brief exposure to cobalt chloride.
authors.affiliation: Department of Pharmacology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Seong
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.forename: Tae-Gyu
authors.initials: TG
authors.lastname: Park
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Seok
authors.forename: Woonyi
authors.initials: W
authors.lastname: Baek
authors.forename: Si-Oh
authors.initials: SO
authors.lastname: Kim
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lim
authors.forename: Dong Heon
authors.initials: DH
authors.lastname: Yang
authors.forename: Inkyeom
authors.initials: I
authors.lastname: Kim