title: Preventative effects of 4,4'-diphenylmethane-bis(methyl) carbamate isolated from cortex mori on human umbilical vein endothelial cell dysfunction induced by advanced glycation end products.
abstract: Advanced glycation end-products (AGEs) have been regarded as an initial motivating factor in the pathogenesis of endothelial dysfunction in diabetic complications. 4,4'-Diphenylmethane-bis(methyl) carbamate (DMPC), a carbamate compound, was isolated from Cortex Mori and its prevention effects against AGEs-induced endothelial dysfunction were studied. 4,4'-Diphenylmethane-bis(methyl) carbamate significantly reduced cell apoptosis to normal level at 10⁻⁹  mol/L concentration. Advanced glycation end-products up-regulated the expression of Bad and Bax and down-regulated Bcl-2 proteins, and pretreatment with DMPC significantly down-regulated Bad and Bax while up-regulating Bcl-2 expressions. In addition, ICAM (intercellular adhesion molecule)-1 and TGF (transforming growth factor)-β1 expressions in human umbilical vein endothelial cell (HUVEC) were significantly enhanced by AGEs. More importantly, these increases of ICAM-1 and TGF-β1 expressions were reduced meaningfully with the pretreatment of DMPC. All the results showed DMPC had prevention effects against the progression of AGE-induced endothelial dysfunction, and this compound might be a promising agent against endothelial dysfunction in diabetic vascular complications.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Biotechnology Labortory of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau. qzhu40@yahoo.com.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: You-Hua
authors.initials: YH
authors.lastname: Xu
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wang
authors.forename: Wai
authors.initials: W
authors.lastname: Au-Yeung
authors.forename: Zhao-Guang
authors.initials: ZG
authors.lastname: Zheng
authors.forename: Ru-Shang
authors.initials: RS
authors.lastname: Wang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Xiang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis; D002219:Carbamates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D017127:Glycation End Products, Advanced / Q000009:adverse effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D031625:Morus / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism; D051025:bcl-Associated Death Protein / Q000235:genetics / Q000378:metabolism
chemical_list: C572821:4,4'-diphenylmethane-bis(methyl)carbamate; D000972:Antineoplastic Agents, Phytogenic; C494819:BAD protein, human; C494826:BAX protein, human; D002219:Carbamates; D017127:Glycation End Products, Advanced; D053773:Transforming Growth Factor beta1; D051028:bcl-2-Associated X Protein; D051025:bcl-Associated Death Protein; D018799:Intercellular Adhesion Molecule-1