title: Epithelial cell patterns on a PDMS polymer surface using a micro plasma structure.
abstract: We developed a simple and reproducible epithelial cell-patterning tool on a PDMS (polydimethylsiloxane) polymer surface using a micro plasma structure that did not require chemical or biological treatment. The concept behind this novel approach was based on the fact that cells should easily adhere to the plasma-treated PDMS surface and not the inherent PDMS surface. The micro plasma structure consisted of copper and SU-8 photoresist on a glass substrate. A predetermined space (micro plasma chamber) was formed between the copper electrode layer on the upper part and the PDMS plate surrounded by the SU-8 photoresist structure. The single cell pattern was achieved by introducing a micro-plasma structure of 30 μm in width. Using this approach, a closed cell pattern was successfully developed using a micro chamber structure that was 200 μm in diameter with a microchannel that was 10 μm in width.
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentisty, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Kim
authors.forename: Soonmin
authors.initials: S
authors.lastname: Seo
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D003300:Copper; D003584:Cytological Techniques; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology*; D004852:Epoxy Compounds; D004867:Equipment Design; D005898:Glass; D006801:Humans; D055616:Microtechnology / Q000379:methods*; D010100:Oxygen; D010316:Particle Size; D058626:Plasma Gases; D011108:Polymers; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D004129:Dimethylpolysiloxanes; D004852:Epoxy Compounds; D058626:Plasma Gases; D011108:Polymers; C520895:SU-8 compound; C013830:baysilon; D003300:Copper; D010100:Oxygen