title: [The calixarene C-107 increases the affinity of the Na+,K(+)-ATPase in plasmatic membrane of smooth muscle cells to the ouabain].
abstract: In the experiments carried out with the suspension of the myometrium cell plasmatic membranes treated with 0.1% digitonin solution we investigated the influence of calixarene C-107 (5,17-diamino(2-pyridyl)methylphosphono-11,23-di-tret-butyl-26,28-dihydroxy-25,27-dipropoxyca-lix[4]arene) on the Na+,K(+)-ATPase activity. It was shown that this calixarene increased the affinity of the enzyme for the sodium pump conventional inhibitor - ouabain: the magnitudes of the seeming constant of inhibition I0.5 changed from 26.9 +/- 1.3 mM to 10.9 +/- 0.6 mM. However the ouabain itself did not influence on the affinity of the Na+,K(+)-ATPase for calixarene C-107.
authors.forename: T O
authors.initials: TO
authors.lastname: Veklich
authors.forename: O A
authors.initials: OA
authors.lastname: Shkrabak
authors.forename: R V
authors.initials: RV
authors.lastname: Rodik
authors.forename: V I
authors.initials: VI
authors.lastname: Kal'chenko
authors.forename: S O
authors.initials: SO
authors.lastname: Kosterin
mesh_terms: D000818:Animals; D047250:Calixarenes / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; C533334:calixarene C-107; D047250:Calixarenes; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase