title: RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks.
abstract: Homologous recombination repairs DNA double-strand breaks by searching for, invading, and copying information from a homologous template, typically the homologous chromosome or sister chromatid. Tight wrapping of DNA around histone octamers, however, impedes access of repair proteins to DNA damage. To facilitate DNA repair, modifications of histones and energy-dependent remodeling of chromatin are required, but the precise mechanisms by which chromatin modification and remodeling enzymes contribute to homologous DNA repair are unknown. Here we have systematically assessed the role of budding yeast RSC (remodel structure of chromatin), an abundant, ATP-dependent chromatin-remodeling complex, in the cellular response to spontaneous and induced DNA damage. RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination. Multiple recombination assays revealed that RSC is uniquely required for recombination between sister chromatids by virtue of its ability to recruit cohesin at DNA breaks and thereby promoting sister chromatid cohesion. This study provides molecular insights into how chromatin remodeling contributes to DNA repair and maintenance of chromatin fidelity in the face of DNA damage.
authors.affiliation: Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center at San Antonio, 15355 Lambda Drive, San Antonio, TX 78245, USA.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Oum
authors.forename: Changhyun
authors.initials: C
authors.lastname: Seong
authors.forename: Youngho
authors.initials: Y
authors.lastname: Kwon
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Ji
authors.forename: Amy
authors.initials: A
authors.lastname: Sid
authors.forename: Sreejith
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Ira
authors.forename: Anna
authors.initials: A
authors.lastname: Malkova
authors.forename: Patrick
authors.initials: P
authors.lastname: Sung
authors.forename: Sang Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Eun Yong
authors.initials: EY
authors.lastname: Shim
mesh_terms: D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D059765:Homologous Recombination; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism; D059767:Recombinational DNA Repair; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000097722:Cohesins
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D051135:Rad51 Recombinase; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000097722:Cohesins; C511067:NPL6 protein, S cerevisiae; C400213:RAD59 protein, S cerevisiae; C103337:RSC complex, S cerevisiae