title: Effect of liposome-encapsulated hemoglobin on antigen-presenting cells in mice.
abstract: Liposome-encapsulated hemoglobin (LEH) is removed from the circulation and degraded in the reticuloendothelial system, including dendritic cells (DCs) and macrophages. Therefore, LEH at a large dose may overload the system, cause a competitive inhibition in antigen-presenting activity, and impair the immune response of the host. Changes in cellularity of immunocompetent cells were monitored serially up to 4 weeks by flow cytometry in wild-type mice receiving 20 mL/kg of LEH, syngeneic red blood cells (RBCs), or saline. DCs were collected from the host spleen 1, 7, and 28 days after receiving the solution and were cocultured with naïve cluster of differentiation 4 T cells from T-cell receptor transgenic mice in the absence or presence of third-party antigens. After LEH administration, the cellularity of DCs and macrophages in the recipient spleen remained unchanged from control mice receiving RBCs or saline. While subset populations and costimulatory molecule expressions were different, DCs from LEH-administered mice expressed high levels of interleukin-2 production and helper T-cell activation in response to a third-party antigen and superantigens, as did the DCs from control mice receiving RBCs or saline. The results suggest that 20 mL/kg of LEH does not greatly alter antigen-presenting activity to third-party antigens.
authors.affiliation: Department of Cell Transplantation and Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan. akira@is.icc.u-tokai.ac.jp
authors.forename: Akira T
authors.initials: AT
authors.lastname: Kawaguchi
authors.forename: Junko
authors.initials: J
authors.lastname: Aokawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Yamada
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Yoshiba
authors.forename: Shunichi
authors.initials: S
authors.lastname: Kato
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kametani
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects* / Q000276:immunology*; D001802:Blood Substitutes / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D039521:CD11c Antigen / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D005260:Female; D007376:Interleukin-2 / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
chemical_list: D001802:Blood Substitutes; D039521:CD11c Antigen; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; D008081:Liposomes