title: Photoresponsive 5'-cap for the reversible photoregulation of gene expression.
abstract: Photoresponsive 5'-caps that can be reversibly cis-trans isomerized by light irradiation were developed for the reversible photoregulation of gene expression. The 8-naphthylvinyl cap (8NV-cap) in the trans form completely inhibited translation of mRNA, whereas the cis form yielded protein with the same efficiency as mRNA capped with the normal-cap, a 26-fold higher efficiency than that of the trans form. The 8NV-capped mRNA could be switched between a translating state (ON state) and a non-translating state (OFF state) in a reversible fashion by alternately irradiating with monochromatic 410 nm or 310 nm light.
authors.affiliation: Innovative use of Light and Materials/Life, JST-PRESTO, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan. o_shinji@riken.jp
authors.forename: Shinzi
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D015870:Gene Expression; D008027:Light; D008958:Models, Molecular; D012315:RNA Caps / Q000737:chemistry / Q000235:genetics*; D013237:Stereoisomerism
chemical_list: D020121:5' Untranslated Regions; D012315:RNA Caps