title: The non-invasive (13)C-methionine breath test detects hepatic mitochondrial dysfunction as a marker of disease activity in non-alcoholic steatohepatitis.
abstract: BACKGROUND
  Mitochondrial dysfunction plays a central role in the general pathogenesis of non-alcoholic fatty liver disease (NAFLD), increasing the risk of developing steatosis and subsequent hepatocellular inflammation. We aimed to assess hepatic mitochondrial function by a non-invasive (13)C-methionine breath test (MeBT) in patients with histologically proven NAFLD.
  
  
  METHODS
  118 NAFLD-patients and 18 healthy controls were examined by MeBT. Liver biopsy specimens were evaluated according to the NASH scoring system.
  
  
  RESULTS
  Higher grades of NASH activity and fibrosis were independently associated with a significant decrease in cumulative (13)C-exhalation (expressed as cPDR(%)). cPDR (1.5h) was markedly declined in patients with NASH and NASH cirrhosis compared to patients with simple steatosis or borderline diagnosis (cPDR1.5h: 3.24 ± 1.12% and 1.32 ± 0.94% vs. 6.36 ± 0.56% and 4.80 ± 0.88% respectively; p<0.001). (13)C-exhalation further declined in the presence of advanced fibrosis which was correlated with NASH activity (r = 0.36). The area under the ROC curve (AUROC) for NASH diagnosis was estimated to be 0.87 in the total cohort and 0.83 in patients with no or mild fibrosis (F0-1).
  
  
  CONCLUSIONS
  The (13)C-methionine breath test indicates mitochondrial dysfunction in non-alcoholic fatty liver disease and predicts higher stages of disease activity. It may, therefore, be a valuable diagnostic addition for longitudinal monitoring of hepatic (mitochondrial) function in non-alcoholic fatty liver disease.
authors.affiliation: Department of Medicine 1, St. Josef-Hospital, University of Bochum, Germany. mbanasch@gmx.de
authors.forename: Matthias
authors.initials: M
authors.lastname: Banasch
authors.forename: M
authors.initials: M
authors.lastname: Ellrichmann
authors.forename: A
authors.initials: A
authors.lastname: Tannapfel
authors.forename: W E
authors.initials: WE
authors.lastname: Schmidt
authors.forename: O
authors.initials: O
authors.lastname: Goetze
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001944:Breath Tests / Q000379:methods*; D002247:Carbon Isotopes; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005234:Fatty Liver / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008715:Methionine / Q000378:metabolism; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000378:metabolism*; D065626:Non-alcoholic Fatty Liver Disease; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D002247:Carbon Isotopes; D008715:Methionine