title: Selection of a synthetic glycan oligomer from a library of DNA-templated fragments against DC-SIGN and inhibition of HIV gp120 binding to dendritic cells.
abstract: We report the synthesis of a nucleic acid-encoded carbohydrate library, its combinatorial self-assembly into 37,485 pairs and a screen against DC-SIGN leading to the identification of consensus ligand motifs. A prototypical example from the selected pairs was shown to have enhanced binding. A dendrimer incorporating the selected motifs inhibited gp120's binding to dendritic cells with higher efficiency than mannan.
authors.affiliation: Institut de Science et Ingenierie Supramoleculaires (ISIS), Université de Strasbourg-CNRS, 8 allee Gaspard Monge, 67000 Strasbourg, France.
authors.forename: Mihai
authors.initials: M
authors.lastname: Ciobanu
authors.forename: Kuo-Ting
authors.initials: KT
authors.lastname: Huang
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Daguer
authors.forename: Sofia
authors.initials: S
authors.lastname: Barluenga
authors.forename: Olivier
authors.initials: O
authors.lastname: Chaloin
authors.forename: Evelyne
authors.initials: E
authors.lastname: Schaeffer
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Mueller
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Mitchell
authors.forename: Nicolas
authors.initials: N
authors.lastname: Winssinger
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D006678:HIV / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism*; D008024:Ligands; D020135:Peptide Nucleic Acids / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D004247:DNA; D015699:HIV Envelope Protein gp120; D037181:Lectins, C-Type; D008024:Ligands; D020135:Peptide Nucleic Acids; D011108:Polymers; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin