title: Radioprotection by Podophyllum hexandrum in the liver of mice: A mechanistic approach.
abstract: To evaluate radiation protection offered by the extract of Podophyllum hexandrum, expression of various cytoprotective proteins was studied using liver of Swiss albino Strain 'A' male mouse by immunoblotting. Induction of heat-shock factor-1 (HSF-1), led to up-regulation of heat-shock protein-70 (HSP-70) upon P. hexandrum (200mg/kg b. wt.; i.p. 50% ethanolic extract) treatment 2h before irradiation (10Gy) as compared to sham-irradiated control. Translocation of free nuclear factor kappa B (NFκB) from cytoplasm to nucleus was found to be inhibited upon P. hexandrum treatment. An increase in Bcl-2, proliferating cell nuclear antigen (PCNA) along with a decrease in p53, caspase-3, apoptosis inducing factor (AIF) expression was observed in the mice treated with P. hexandrum. The present study indicated that P. hexandrum extract provides protection from radiation by modulation of expression of the proteins associated with apoptosis.
authors.affiliation: Division of Radiopharmaceuticals and Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Marg, Delhi 110054, India.
authors.forename: Raj
authors.initials: R
authors.lastname: Kumar
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Singh
authors.forename: Rajesh
authors.initials: R
authors.lastname: Arora
authors.forename: Ashok
authors.initials: A
authors.lastname: Sharma
authors.forename: Jagdish
authors.initials: J
authors.lastname: Prasad
authors.forename: Ravinder
authors.initials: R
authors.lastname: Sagar
authors.forename: Surendar
authors.initials: S
authors.lastname: Singh
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma