title: [Signal transduction pathway for TNF-induced IL-6 release from mast cells].
abstract: OBJECTIVE
  To investigate the effect of TNF on release of IL-6, IL-10 and histamine from mast cells and to explore its potential signal transduction pathway.
  
  
  METHODS
  After stimulation of various concentrations of TNF, the P815 cells were analyzed by cellular activation of signal ELISA(CASE) to detect phosphorylation of ERK, p38 and STAT3, and the supernatants were collected and analyzed by ELISA to quantify release of IL-6, IL-10 and histamine from the cells.
  
  
  RESULTS
  Compared with the untreated control, increased IL-6 release was detected in TNF-stimulated P815 cells (P<0.05). Pretreatment of PD98059 or U0126 inhibited TNF-induced IL-6 release and ERK phosphorylation in P815 cells (P<0.05), whereas pretreatment of SB203580 or AG490 hardly affect IL-6 release, with little effect on phosphorylation of p38 and STAT3 respectively.
  
  
  CONCLUSIONS
  TNF induced IL-6 release from P815 cells may involve activation of ERK signalling pathway.
authors.affiliation: Department of Pathophysiology, Hainan Medical College, Haikou 571101, China. zhanghuiyun2003@126.com
authors.forename: Hui-yun
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xing-nan
authors.initials: XN
authors.lastname: Long
authors.forename: Shao-heng
authors.initials: SH
authors.lastname: He
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology; D006636:Histamine Release / Q000276:immunology; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D010766:Phosphorylation / Q000187:drug effects; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D005419:Flavonoids; D015850:Interleukin-6; D047428:Protein Kinase Inhibitors; D050796:STAT3 Transcription Factor; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one