title: Purification, crystallization and X-ray diffraction study of extracellular dermal glycoprotein from carrot and the inhibition complex that it forms with an endo-β-glucanase from Aspergillus aculeatus.
abstract: Extracellular dermal glycoprotein (EDGP) may play an important role in the plant defence system of the carrot (Daucus carota) as it has inhibitory activity against endo-β-glucanase produced by invading pathogens. Here, EDGP and the inhibition complex that it forms with FI-CMCase, a carboxyl methyl cellulase from Aspergillus aculeatus, were successfully crystallized. The hexagonal crystal of EDGP belonged to space group P6(2), with unit-cell parameters a=b=130.4, c=44.5 Å, γ=120°. The monoclinic crystal of the complex of EDGP with FI-CMCase belonged to space group C2, with unit-cell parameters a=169.5, b=143.0, c=63.0 Å, β=110.9°.
authors.affiliation: Graduate School of Nanobioscience, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshizawa
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hirano
authors.forename: Mamoru
authors.initials: M
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hashimoto
mesh_terms: D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology*; D002480:Cellulase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D003460:Crystallization; D018552:Daucus carota / Q000737:chemistry* / Q000378:metabolism; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D014961:X-Ray Diffraction
chemical_list: D006023:Glycoproteins; D002480:Cellulase