title: Excess dietary sodium selenite alters apoptotic population and oxidative stress markers of spleens in broilers.
abstract: Three hundred 1-day-old avian broilers were fed on a basic diet (0.2 mg/kg selenium) or the same diet amended to contain 1, 5, 10, and 15 mg/kg selenium supplied as sodium selenite (n = 60/group). In comparison with those of 0.2 mg/kg selenium group, the percentages of annexin V-positive splenocytes were increased in 5, 10, and 15 mg/kg selenium groups. TUNEL assay revealed that apoptotic cells with brown-stained nuclei distributed within the red pulp and white pulp of the spleens with increased frequency of occurrence in 10 and 15 mg/kg selenium groups in comparison with that of 0.2 mg/kg Se group. Sodium selenite-induced oxidative stress in spleens of chickens was evidenced by decrease in glutathione peroxidase, superoxide dismutase, and catalase activities and increase in malondialdehyde contents. The results indicate that excess dietary selenium in the range of 5-15 mg/kg of feed causes oxidative stress, which may be mainly responsible for the increased apoptosis of splenocytes in chickens.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan 625014, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Yaxin
authors.initials: Y
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Pan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects*; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005979:Glutathione Peroxidase / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D018038:Sodium Selenite / Q000008:administration & dosage / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D018038:Sodium Selenite