title: Fast fluorescence techniques for crystallography beamlines.
abstract: This paper reports on several developments of X-ray fluorescence techniques for macromolecular crystallography recently implemented at the National Institute of General Medical Sciences and National Cancer Institute beamlines at the Advanced Photon Source. These include (i) three-band on-the-fly energy scanning around absorption edges with adaptive positioning of the fine-step band calculated from a coarse pass; (ii) on-the-fly X-ray fluorescence rastering over rectangular domains for locating small and invisible crystals with a shuttle-scanning option for increased speed; (iii) fluorescence rastering over user-specified multi-segmented polygons; and (iv) automatic signal optimization for reduced radiation damage of samples.
authors.forename: Sergey
authors.initials: S
authors.lastname: Stepanov
authors.forename: Mark
authors.initials: M
authors.lastname: Hilgart
authors.forename: Derek W
authors.initials: DW
authors.lastname: Yoder
authors.forename: Oleg
authors.initials: O
authors.lastname: Makarov
authors.forename: Michael
authors.initials: M
authors.lastname: Becker
authors.forename: Ruslan
authors.initials: R
authors.lastname: Sanishvili
authors.forename: Craig M
authors.initials: CM
authors.lastname: Ogata
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Venugopalan
authors.forename: David
authors.initials: D
authors.lastname: Aragão
authors.forename: Martin
authors.initials: M
authors.lastname: Caffrey
authors.forename: Janet L
authors.initials: JL
authors.lastname: Smith
authors.forename: Robert F
authors.initials: RF
authors.lastname: Fischetti