title: Coordination conjugates of therapeutic proteins with drug carriers: a new approach for versatile advanced drug delivery.
abstract: We present here a general system for the coordination attachment of therapeutic proteins to a drug delivery system and its application in combined therapy. Proof of concept is demonstrated by the synthesis and testing of the targeted drug delivery system for cytostatics, which is based on a combination of the drug carrier Zn-porphyrin-cyclodextrin conjugates and their supramolecular coordination complexes with immunoglobulins. This system can be as readily used for a variety of therapeutic and targeting proteins including PAs, MAs, lectins, and HSA. Moreover, it allows combined photodynamic therapy, cell targeted chemotherapy and immunotherapy. When tested in a mouse model with human C32 carcinoma, the therapeutic superiority of the coordination assembly nanosystem was shown in comparison with the efficacy of building blocks used for the construction of the system.
authors.affiliation: Institute of Chemical Technology, Prague, Technická 5, 166 28 Prague 6, Czech Republic.
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Kejík
authors.forename: Tomáš
authors.initials: T
authors.lastname: Bříza
authors.forename: Jarmila
authors.initials: J
authors.lastname: Králová
authors.forename: Pavla
authors.initials: P
authors.lastname: Poučková
authors.forename: Aleš
authors.initials: A
authors.lastname: Král
authors.forename: Pavel
authors.initials: P
authors.lastname: Martásek
authors.forename: Vladimír
authors.initials: V
authors.lastname: Král
mesh_terms: D000818:Animals; D003505:Cyclodextrins / Q000737:chemistry*; D054697:Cytostatic Agents / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000276:immunology; D008665:Metalloporphyrins / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D008968:Molecular Conformation; D015032:Zinc / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D054697:Cytostatic Agents; D004337:Drug Carriers; D007136:Immunoglobulins; D008665:Metalloporphyrins; D015032:Zinc