title: Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2).
abstract: Four germicidin homologs were isolated from a liquid culture of Streptomyces coelicolor A3(2). These were identified as germicidins A, B and C, and surugapyrone A (germicidin D). Absolute stereochemistry of the chiral center in germicidins A and C is determined to be S. All germicidins inhibited germination of S. coelicolor A3(2) spores above 1 μg ml(-1). S. coelicolor A3(2) spores collected from a single petri dish (9 cm i.d.) contained 5.4 μg of germicidin A (-2.7 × 10(-14) g per spore), which accounts for 2.3% of the spore extract, and contents of germicidins B, C and D were 0.2-0.8 μg. The activity of the spore extract corresponded well with the sum of the activity of each germicidin, which was estimated from the content and dose-response curve, which indicates that germicidins functions as self-germination inhibitors in S. coelicolor A3(2). Inhibitory action of germicidin A on spore germination was reversible and germicidin A inhibited not only spore germination but also hyphal elongation.
authors.affiliation: Science of Plant and Animal Production Course, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Aoki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsumoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawaide
authors.forename: Masahiro
authors.initials: M
authors.lastname: Natsume
mesh_terms: D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013171:Spores, Bacterial / Q000254:growth & development*; D048372:Streptomyces coelicolor / Q000378:metabolism*
chemical_list: D011753:Pyrones; C551568:surugapyrone A; C082790:germicidin