title: [Identification and characterization of a novel hydrocarbon-degrading Marinobacter sp. PY97S].
abstract: OBJECTIVE
  To identify and characterize a hydrocarbon-degrading bacterium isolated from the sediment of the Yellow Sea.
  
  
  METHODS
  We used 16S rRNA gene sequences based phylogenetic analysis, physiological and biochemical characterization, DNA G + C content assaying, determination of cellular fatty acids, testing of carbon sources and respiratory lipoquinone and experiment of DNA-DNA relatedness. Its capability of degrading aliphatic hydrocarbons in ONR7a media supplemented with nine n-alkanes, separately, as sole source of carbon and energy was further determined.
  
  
  RESULTS
  The Gram-negative isolate PY97S was a member of the genus Marinobacter, catalase-and oxidase-positive, and with Q-9 as its predominant respiratory lipoquinone. The similarity between its 16S rRNA gene and that of its most closely related type strain in GenBank Marinobacter koreensis DD-M3(T) was 96.93%, and their level of DNA relatedness was 46.7%. The appropriate temperature for its growth ranged from 15 degrees C to 35 degrees C with the optimum of 30 degrees C, the appropriate initial acidity from pH 6.0 to 9.5 with the optimum of pH 7.0, and the appropriate salinity (NaCl) from 0% to 10% with the optimum of 0%. It metabolized many carbohydrates and organic acids and was sensitive to diverse antibiotics including ampicillin and piperacillin. The G + C content of its genomic DNA was 48.2 mol%. The major fatty acids were 2-methyl C15:0 (29.97%), C16: 1omega7c (27.22%), C12:0 (22.22%) and C16: 1omega9c (5.73%).
  
  
  CONCLUSIONS
  The isolate PY97S was identified as a petroleum hydrocarbon-degrading novel species of genus Marinobacter, holding the potential of being applied in the bioremediation of oil spill.
authors.affiliation: College of Life Science, Ludong University, Yantai 264025, China. liqian_850730@163.com
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Zhisong
authors.initials: Z
authors.lastname: Cui
authors.forename: Aifen
authors.initials: A
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D001483:Base Sequence; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006838:Hydrocarbons / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D053520:Marinobacter / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006838:Hydrocarbons; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D002244:Carbon