title: [Research of biocompatibility of nanoparticles with fluorescent domen Er/Yb in the neutrophilic granulocytes system].
abstract: Fluorescent tags are extensively used for the diagnostic, labeling and marking in vivo and in vitro systems. In this study, fluorescent nanoparticles with Er/Yb lightning center were tested on neutrophilic granulocytes. The main purpose was to idenfity possible toxic effect. The negative impact of fluorophores on the metabolism of neutrophilic and their enzyme systems, on the receptor-mediated cell responses and on the rigidity of cell membranes was shown. The viability of neutrophils (estimated with the use of propidium iodide) after 2 hours of incubation with the fluorescent nanoparticles in concentrations of 10(-4) and 10(-3) mM was 27.0 +/- 6.6 and 19.07 +/- 3.34 %, respectively.
authors.forename: S N
authors.initials: SN
authors.lastname: Pleskova
authors.forename: E N
authors.initials: EN
authors.lastname: Gorshkova
authors.forename: É P
authors.initials: ÉP
authors.lastname: Mikheeva
authors.forename: A N
authors.initials: AN
authors.lastname: Shushunov
mesh_terms: D001391:Azo Compounds / Q000032:analysis; D002462:Cell Membrane / Q000187:drug effects*; D019069:Cell Respiration / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D004871:Erbium / Q000009:adverse effects / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D006867:Hydrolases / Q000032:analysis / Q000378:metabolism; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000009:adverse effects*; D049329:Nanostructures / Q000009:adverse effects; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D011419:Propidium / Q000032:analysis; D045663:Quantum Dots; D014343:Trypan Blue / Q000032:analysis; D015018:Ytterbium / Q000009:adverse effects / Q000378:metabolism
chemical_list: D001391:Azo Compounds; D005456:Fluorescent Dyes; C033302:diformazan dye; D011419:Propidium; D004871:Erbium; D006867:Hydrolases; D014343:Trypan Blue; D015018:Ytterbium