title: Defining genome maintenance pathways using functional genomic approaches.
abstract: Genome maintenance activities including DNA repair, cell division cycle control, and checkpoint signaling pathways preserve genome integrity and prevent disease. Defects in these pathways cause birth defects, neurodegeneration, premature aging, and cancer. Recent technical advances in functional genomic approaches such as expression profiling, proteomics, and RNA interference (RNAi) technologies have rapidly expanded our knowledge of the proteins that work in these pathways. In this review, we examine the use of these high-throughput methodologies in higher eukaryotic organisms for the interrogation of genome maintenance activities.
authors.affiliation: Department of Biochemistry, Vanderbilt University School of Medicine, Light Hall, Nashville, TN 37232, USA.
authors.forename: Carol E
authors.initials: CE
authors.lastname: Bansbach
authors.forename: David
authors.initials: D
authors.lastname: Cortez
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D013058:Mass Spectrometry; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods; D034622:RNA Interference; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques
chemical_list: D018797:Cell Cycle Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins