title: Responses of HSC70 expression in diencephalon to iron deficiency anemia in rats.
abstract: A powdered diet containing 100 or 3 ppm Fe was fed to rats starting at the age of 3 weeks. The voluntary activity level was checked using a wheel in the cage during the 17th week after the beginning of supplementation. Significantly less activity was seen in the 3 ppm Fe group during both light and dark periods. After 20 weeks, the blood and diencephalon were sampled from both groups. Lower hematocrit and blood hemoglobin content was observed in the 3 ppm Fe group. The level of 70 kDa heat shock cognate (HSC70) expression was greater in the diencephalon of the 3 ppm Fe group. In addition, the distribution of HSC70 was determined by proximity ligation assay. More HSC70-positive as well as total cells were noted in several areas of the diencephalon of the iron-deficient rats. The altered expression and distribution of HSC70 might play some role in the neurological changes.
authors.affiliation: Section of Applied Physiology, Graduate School of Medicine, Osaka University, Toyonaka, Osaka, 560-0043, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kawano
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Oke
authors.forename: Sachiko
authors.initials: S
authors.lastname: Nomura
authors.forename: Ryo
authors.initials: R
authors.lastname: Fujita
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohira
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakai
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Ohira
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004027:Diencephalon / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D006400:Hematocrit / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism; D019266:Iron, Dietary; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050883:HSC70 Heat-Shock Proteins; D006454:Hemoglobins; C494373:Hspa8 protein, rat; D019266:Iron, Dietary