title: Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.
abstract: We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
authors.affiliation: Department of Biology, University of Texas of the Permian Basin, Odessa, TX 79762-0001, USA.
authors.forename: B J Z
authors.initials: BJ
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Gutierrez
authors.forename: E
authors.initials: E
authors.lastname: Guerrero
authors.forename: C J
authors.initials: CJ
authors.lastname: Brewer
authors.forename: D P
authors.initials: DP
authors.lastname: Henderson
mesh_terms: D019149:Bioreactors; D053898:Biosynthetic Pathways / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006418:Heme / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D010957:Plasmids; D016981:Plesiomonas / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D006454:Hemoglobins; D011994:Recombinant Proteins; D006418:Heme