title: CBP/p300 and SIRT1 are involved in transcriptional regulation of S-phase specific histone genes.
abstract: BACKGROUND
  Histones constitute a type of essential nuclear proteins important for chromatin structure and functions. The expression of major histones is strictly confined to the S phase of a cell cycle and tightly coupled to DNA replication.
  
  
  RESULTS
  With RT-qPCR and ChIP assays, we investigated transcriptional regulation of the S-phase specific histone genes and found that the acetylation level of histones on core histone gene promoters fluctuated during cell cycle in a pattern similar to RNA polymerase II association. Further, we showed that CBP/p300 and SIRT1 were recruited to histone gene promoters in an NPAT-dependent manner, knockdown of which affected histone acetylation on histone gene promoters and histone gene transcription.
  
  
  CONCLUSIONS
  These observations contribute to further understanding of the mechanism by which the expression of canonical histone genes is regulated, and also implicate a link between histone expression and DNA damage repair and cell metabolism.
authors.affiliation: Institute of Molecular and Cell Biology, Singapore, Singapore.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: He
authors.forename: Fa-Xing
authors.initials: FX
authors.lastname: Yu
authors.forename: Chi
authors.initials: C
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000107:Acetylation; D050882:CREB-Binding Protein / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism; D049109:Cell Proliferation; D050881:E1A-Associated p300 Protein / Q000378:metabolism*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D016196:S Phase / Q000235:genetics*; D056564:Sirtuin 1 / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D018797:Cell Cycle Proteins; D006657:Histones; C101693:NPAT protein, human; D009687:Nuclear Proteins; D050882:CREB-Binding Protein; C494363:CREBBP protein, human; D050881:E1A-Associated p300 Protein; C494360:EP300 protein, human; D012319:RNA Polymerase II; C447939:SIRT1 protein, human; D056564:Sirtuin 1