title: Gene-protein correlation in single cells.
abstract: We present a novel methodology combining traditional fluorescent in situ hybridization with an in situ protein detection technology called proximity ligation assay. This method has potential to perform a detailed analysis of the relationship between gene status and corresponding protein expression in cells and tissues. We demonstrate that the fluorescent in situ gene protein assay methodology is capable of resolving gene and protein patterns simultaneously on a cell-by-cell basis.
authors.affiliation: Northwestern Brain Tumor Institute, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors.forename: Jaclyn J
authors.initials: JJ
authors.lastname: Renfrow
authors.forename: Adrienne C
authors.initials: AC
authors.lastname: Scheck
authors.forename: Neil S
authors.initials: NS
authors.lastname: Dhawan
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Lukac
authors.forename: Hannes
authors.initials: H
authors.lastname: Vogel
authors.forename: James P
authors.initials: JP
authors.lastname: Chandler
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Raizer
authors.forename: Griffith R
authors.initials: GR
authors.lastname: Harsh
authors.forename: Arnab
authors.initials: A
authors.lastname: Chakravarti
authors.forename: Markus
authors.initials: M
authors.lastname: Bredel
mesh_terms: D001932:Brain Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism*; D005784:Gene Amplification; D005909:Glioblastoma / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D040901:Proteomics; D014407:Tumor Cells, Cultured
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors