title: Softness of atherogenic lipoproteins: a comparison of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) using elastic incoherent neutron scattering (EINS).
abstract: Apolipoprotein B100 (apoB100)-containing plasma lipoproteins (LDL and VLDL) supply tissues and cells with cholesterol and fat. During lipolytic conversion from VLDL to LDL the size and chemical composition of the particles change, but the apoB100 molecule remains bound to the lipids and regulates the receptor mediated uptake. The molecular physical parameters which control lipoprotein remodeling and enable particle stabilization by apoB100 are largely unknown. Here, we have compared the molecular dynamics and elasticities of VLDL and LDL derived by elastic neutron scattering temperature scans. We have determined thermal motions, dynamical transitions, and molecular fluctuations, which reflect the temperature-dependent motional coupling between lipid and protein. Our results revealed that lipoprotein particles are extremely soft and flexible. We found substantial differences in the molecular resiliences of lipoproteins, especially at higher temperatures. These discrepancies not only can be explained in terms of lipid composition and mobility but also suggest that apoB100 displays different dynamics dependent on the lipoprotein it is bound to. Hence, we suppose that the inherent conformational flexibility of apoB100 permits particle stabilization upon lipid exchange, whereas the dynamic coupling between protein and lipids might be a key determinant for lipoprotein conversion and atherogenicity.
authors.affiliation: Institute of Biophysics and Nanosystems Research, Austrian Academy of Sciences, Graz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Mikl
authors.forename: Judith
authors.initials: J
authors.lastname: Peters
authors.forename: Marcus
authors.initials: M
authors.lastname: Trapp
authors.forename: Karin
authors.initials: K
authors.lastname: Kornmueller
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Schneider
authors.forename: Ruth
authors.initials: R
authors.lastname: Prassl
mesh_terms: D004548:Elasticity; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry*; D008079:Lipoproteins, VLDL / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D009038:Motion; D033363:Neutron Diffraction / Q000379:methods; D013696:Temperature
chemical_list: D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL