title: Expression of carotenogenic genes and astaxanthin production in Xanthophyllomyces dendrorhous as a function of oxygen tension.
abstract: This report gives an insight into the specific changes in the transcription of four key carotenogenic genes [encoding geranylgeranyl diphosphate synthase (crtE), phytoene desaturase (crtI), phytoene synthase lycopene cyclase (crtYB), and astaxanthin synthase (ast), respectively] in Xanthophyllomyces dendrorhous cultures, with regard to dissolved oxygen (DO) contents of 10%, 25%, and 40% air saturation, respectively. 25% DO proved to be the most beneficial for yeast growth, transcription of carotenogenic genes, and astaxanthin content.
authors.affiliation: School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Mingbo
authors.initials: M
authors.lastname: Lu
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
mesh_terms: D001487:Basidiomycota / Q000235:genetics*; D002338:Carotenoids / Q000096:biosynthesis*; D005800:Genes, Fungal; D010100:Oxygen / Q000378:metabolism*; D012331:RNA, Fungal / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D024341:Xanthophylls / Q000096:biosynthesis
chemical_list: D012331:RNA, Fungal; D024341:Xanthophylls; D002338:Carotenoids; C005948:astaxanthine; D010100:Oxygen