title: Specificity of PCR and serological assays in the detection of Escherichia coli Shiga toxin subtypes.
abstract: Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.
authors.affiliation: Division of Microbiology, FDA, College Park, MD 20740, USA. peter.feng@fda.hhs.gov
authors.forename: Peter C H
authors.initials: PC
authors.lastname: Feng
authors.forename: Karen
authors.initials: K
authors.lastname: Jinneman
authors.forename: Flemming
authors.initials: F
authors.lastname: Scheutz
authors.forename: Steven R
authors.initials: SR
authors.lastname: Monday
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004926:Escherichia coli / Q000472:pathogenicity*; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods; D012703:Serotyping; D022621:Shiga Toxin / Q000032:analysis* / Q000145:classification / Q000235:genetics / Q000276:immunology
chemical_list: D022621:Shiga Toxin