title: New avenue for mid-UV-range detection of underivatized carbohydrates and amino acids in capillary electrophoresis.
abstract: Capillary electrophoresis (CE) appeared as an interesting alternative to chromatographic methods for carbohydrate analysis, but it can be difficult to implement, because of the lack of easily ionizable functions and chromophore groups. Recently, a promising method was proposed by Rovio et al. for the CE separation under extremely high alkaline conditions of neutral carbohydrates under their alcoholate form and their direct UV detection [Rovio et al. Electrophoresis 2007, 28, 3129-3135; and Rovio et al. J. Chromatogr. A 2008, 1185, 139-144], which is claimed to be due to the absorption of enediolate at 270 nm. Even so, most of the detected compounds in Rovio's paper (for example, sucrose) cannot give such enediolate, lacking a carbonyl group. In this work, a deeper insight was paid to the understanding of detection mechanism. In effect, unusual detection phenomena were observed in comparing reducing and nonreducing carbohydrate behaviors, which pointed to the existence of photochemical reactions in the detection window. A more systematic study of the influence of many parameters (carbohydrate nature, electrolyte pH, residence time in the detection window, and capillary diameter) was undertaken. In addition to this, most of this work was performed under cathodic (reversed) electro-osmotic flow conditions (using Polybrene-modified capillaries), to obtain much faster separations than under Rovio's conditions. This study also opens up new avenues for the detection in mid-UV range of non-UV-absorbing compounds bearing reducing moieties, such as amino acids.
authors.affiliation: Central Laboratory of the Prefecture de Police, 39 bis, rue de Dantzig, 75015 Paris, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Sarazin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Delaunay
authors.forename: Christine
authors.initials: C
authors.lastname: Costanza
authors.forename: Véronique
authors.initials: V
authors.lastname: Eudes
authors.forename: Jean-Maurice
authors.initials: JM
authors.lastname: Mallet
authors.forename: Pierre
authors.initials: P
authors.lastname: Gareil
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis*; D019075:Electrophoresis, Capillary; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D002241:Carbohydrates