title: Anticonvulsant and anxiolytic evaluation of leaf extracts of Ocimum gratissimum, a culinary herb.
abstract: Anticonvulsant and anxiolytic activities of leaf extracts and fraction of Ocimum gratissimum L. (Lamiaceae) were studied using seizures induced by pentylenetetrazol and open-field tests in mice. The results showed that the extracts and fraction increased the latency of tonic and tonic-clonic seizures and death and elicited 50% protection against mortality. In the open-field test, the extracts and fraction decreased the frequency of line crossing, center square entries, rearing against a wall and grooming, whereas grooming duration and freezing frequency and duration were increased. Acute toxicity test in mice gave an oral LD(50) greater than 5000 mg/kg for the methanol extract. These findings suggest that extracts of this plant possess anticonvulsant and anxiolytic-like properties.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka - 410 001, Enugu State, Nigeria.
authors.forename: C O
authors.initials: CO
authors.lastname: Okoli
authors.forename: A C
authors.initials: AC
authors.lastname: Ezike
authors.forename: O C
authors.initials: OC
authors.lastname: Agwagah
authors.forename: P A
authors.initials: PA
authors.lastname: Akah