title: Superior therapeutic potential of young bone marrow mesenchymal stem cells by direct intramyocardial delivery in aged recipients with acute myocardial infarction: in vitro and in vivo investigation.
abstract: Introduction. Bone-marrow-derived mesenchymal stem cells (MSCs) have been studied for treatment of myocardial infarction (MI). Since MSCs from older donors show quantitative and qualitative senescent changes, we hypothesized that a better outcome may be achieved if aged recipients are given MSCs obtained from young donors, rather than using their own autologous MSCs. Methods. In vitro studies compared properties of young and old MSCs. Aged rats randomized into 3 groups underwent coronary artery ligations and were then injected with either old (O) or young (Y) MSCs, or ligation alone. Echocardiography evaluated ejection fractions (EF). At 16 weeks, scar deposition was analyzed. Results. Old MSCs exhibited decreased cell viability, proliferation, and differentiation potentials. EF significantly improved early in both cell therapy groups (P < .05). However, at later stages of the study, group Y showed significantly better function which correlated with decreased scar deposition. Conclusions. The significant difference between young and old cells indicates the possible advantage for allotransplanting MSCs from young donors to elderly patients with MI.
authors.affiliation: Divisions of Cardiac Surgery and Surgical Research, Department of Surgery, Faculty of Medicine, McGill University, Montreal, QC, Canada H3G 1A4.
authors.forename: Madhur
authors.initials: M
authors.lastname: Nayan
authors.forename: Arghya
authors.initials: A
authors.lastname: Paul
authors.forename: Guangyong
authors.initials: G
authors.lastname: Chen
authors.forename: Ray C J
authors.initials: RC
authors.lastname: Chiu
authors.forename: Satya
authors.initials: S
authors.lastname: Prakash
authors.forename: Dominique
authors.initials: D
authors.lastname: Shum-Tim