title: Biophysical characterization of complexes of DNA with mixtures of the neutral lipids 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine and 1,2-dioleoyl-sn-glycero-3-phosphocholine in the presence of bivalent metal cations for DNA transfection.
abstract: Neutral lipids have received up to now a little attention as genetic material carriers, despite some valuable features, such as the absence of toxicity and the high stability in serum of their complexes with DNA. We have prepared two quaternary complexes of DNA and mixtures of 1, 2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-hexanoylamine (6PE) or 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine (12PE) with DOPC in aqueous dispersions of bivalent metal cations (PE/DOPC-DNA-M(2+)). The aim was to evaluate the effect of the amide moiety on the transfection efficiency. These complexes form in a self-assembled manner, the DNA condensation being promoted by the metal cations. Synchrotron X-ray diffraction analysis was used to determine the structure of the complexes, which exhibit the lamellar symmetry of the L(α)(c) phase. The size and surface charge of the complexes have also been measured, and promising results of DNA transfections in vitro have been reported.
authors.affiliation: Chemistry Division of the ISAC Department, Polytechnic University of Marche, Via Brecce Bianche, 60131 Ancona, Italy. m.pisani@univpm.it
authors.forename: Michela
authors.initials: M
authors.lastname: Pisani
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mobbili
authors.forename: Immacolata F
authors.initials: IF
authors.lastname: Placentino
authors.forename: Arianna
authors.initials: A
authors.lastname: Smorlesi
authors.forename: Paolo
authors.initials: P
authors.lastname: Bruni
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D008968:Molecular Conformation; D041681:NIH 3T3 Cells; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: C568743:1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-dodecanoylamine; D004337:Drug Carriers; D008670:Metals; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA; C017251:1,2-oleoylphosphatidylcholine