title: Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation.
abstract: Orientedly bioconjugated core/shell Fe(3)O(4)@Au magnetic nanoparticles were synthesized for cell separation. The Fe(3)O(4)@Au magnetic nanoparticles were synthesized by reducing HAuCl(4) on the surfaces of Fe(3)O(4) nanoparticles, which were further characterized in detail by TEM, XRD and UV-vis spectra. Anti-CD3 monoclonal antibody was orientedly bioconjugated to the surface of Fe(3)O(4)@Au nanoparticles through affinity binding between the Fc portion of the antibody and protein A that covalently immobilized on the nanoparticles. The oriented immobilization method was performed to compare its efficiency for cell separation with the non-oriented one, in which the antibody was directly immobilized onto the carboxylated nanoparticle surface. Results showed that the orientedly bioconjugated Fe(3)O(4)@Au MNPs successfully pulled down CD3(+) T cells from the whole splenocytes with high efficiency of up to 98.4%, showing a more effective cell-capture nanostructure than that obtained by non-oriented strategy. This developed strategy for the synthesis and oriented bioconjugation of Fe(3)O(4)@Au MNPs provides an efficient tool for cell separation, and may be further applied to various fields of bioanalytical chemistry for diagnosis, affinity extraction and biosensor.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Biochemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University, Beijing, China.
authors.forename: Yi-Ran
authors.initials: YR
authors.lastname: Cui
authors.forename: Chao
authors.initials: C
authors.lastname: Hong
authors.forename: Ying-Lin
authors.initials: YL
authors.lastname: Zhou
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Gao
authors.forename: Xin-Xiang
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D006046:Gold