title: Mycobacterium koreense sp. nov., a slowly growing non-chromogenic species closely related to Mycobacterium triviale.
abstract: A novel slow-growing, non-chromogenic mycobacterium (strain 01-305(T)) was isolated from a patient with pulmonary dysfunction. Growth characteristics, acid-fastness and the results of 16S rRNA gene sequencing supported the placement of this strain within the genus Mycobacterium. Phenotypically, strain 01-305(T) was generally similar to Mycobacterium triviale ATCC 23292(T), but some unique biochemical characteristics were observed. The 16S rRNA gene sequence of strain 01-305(T) was similar to those of M. triviale ATCC 23290 (GenBank accession no. AY734996, 99.9 % similarity) and M. triviale ATCC 23291 (AY734995, 99.9 %); however, it differed substantially from that of M. triviale ATCC 23292(T) (X88924, 98.2 %). Phylogenetic analysis based on 16S rRNA gene sequences placed strain 01-305(T) in the slow-growing Mycobacterium group close to M. triviale ATCC 23290 and M. triviale ATCC 23291, but not M. triviale ATCC 23292(T). Unique mycolic acid profiles and phylogenetic analysis based on two different chronometer molecules, and the hsp65 and rpoB genes, strongly supported the taxonomic status of this strain as representing a distinct species. These data support the conclusion that strain 01-305(T) represents a novel mycobacterial species, for which the name Mycobacterium koreense sp. nov. is proposed. The type strain is 01-305(T) ( = DSM 45576(T) = KCTC 19819(T)).
authors.affiliation: Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
authors.forename: Byoung-Jun
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, Ulsan, Republic of Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Jeong
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, Ulsan, Republic of Korea.
authors.forename: Seon Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Ulsan University Hospital, Ulsan, Republic of Korea.
authors.forename: Sung-Ryul
authors.initials: SR
authors.lastname: Kim
authors.affiliation: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Republic of Korea.
authors.forename: Hee-Kyung
authors.initials: HK
authors.lastname: Yu
authors.affiliation: The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul 137-140, Republic of Korea.
authors.forename: Young-Gil
authors.initials: YG
authors.lastname: Park
authors.affiliation: Department of Microbiology, School of Medicine, Joong-Ang University, Seoul, Republic of Korea.
authors.forename: Ki-Jeong
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
authors.forename: Yoon-Hoh
authors.initials: YH
authors.lastname: Kook
authors.affiliation: Department of Microbiology and Immunology, Cancer Research Institute and Liver Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea.
authors.forename: Bum-Joon
authors.initials: BJ
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S