title: The proteome of mouse brain microvessel membranes and basal lamina.
abstract: The blood-brain barrier (BBB) is a multicellular vascular structure separating blood from the brain parenchyma that is composed of endothelial cells with tight intercellular junctions, surrounded by a basal lamina, astrocytes, and pericytes. Previous studies have generated detailed databases of the microvessel transcriptome; however, less information is available on the BBB at the protein level. In this study, we specifically focused on characterization of the membrane fraction of cells within the BBB to generate a more complete understanding of membrane transporters, tight junction proteins, and associated extracellular matrix proteins that are functional hallmarks of the BBB. We used Multidimensional Protein Identification Technology to identify a total of 1,143 proteins in mouse brain microvessels, of which 53% were determined to be membrane associated. Analyses of specific classes of BBB-associated proteins in the context of recent transcriptome reports provide a unique database to assess the relative contribution of genes at the level of both RNA and protein in the maintenance of normal BBB integrity.
authors.affiliation: Department of Surgery, School of Medicine, University of California San Diego, San Diego, California 92103, USA.
authors.forename: Hyun Bae
authors.initials: HB
authors.lastname: Chun
authors.forename: Michael
authors.initials: M
authors.lastname: Scott
authors.forename: Sherry
authors.initials: S
authors.lastname: Niessen
authors.forename: Heather
authors.initials: H
authors.lastname: Hoover
authors.forename: Andrew
authors.initials: A
authors.lastname: Baird
authors.forename: John
authors.initials: J
authors.lastname: Yates
authors.forename: Bruce E
authors.initials: BE
authors.lastname: Torbett
authors.forename: Brian P
authors.initials: BP
authors.lastname: Eliceiri
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D015415:Biomarkers; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism; D001923:Brain Chemistry / Q000235:genetics*; D002196:Capillaries / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D042783:Endothelial Cells / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D016023:Integrins / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D011509:Proteoglycans / Q000378:metabolism; D020543:Proteome / Q000235:genetics*; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D016023:Integrins; D011509:Proteoglycans; D020543:Proteome