title: Increased numbers of oligodendrocyte lineage cells in the optic nerves of cerebroside sulfotransferase knockout mice.
abstract: Sulfatide is a myelin glycolipid that functions in the formation of paranodal axo-glial junctions in vivo and in the regulation of oligodendrocyte differentiation in vitro. Cerebroside sulfotransferase (CST) catalyzes the production of two sulfated glycolipids, sulfatide and proligodendroblast antigen, in oligodendrocyte lineage cells. Recent studies have demonstrated significant increases in oligodendrocytes from the myelination stage through adulthood in brain and spinal cord under CST-deficient conditions. However, whether these result from excess migration or in situ proliferation during development is undetermined. In the present study, CST-deficient optic nerves were used to examine migration and proliferation of oligodendrocyte precursor cells (OPCs) under sulfated glycolipid-deficient conditions. In adults, more NG2-positive OPCs and fully differentiated cells were observed. In developing optic nerves, the number of cells at the leading edge of migration was similar in CST-deficient and wild-type mice. However, BrdU(+) proliferating OPCs were more abundant in CST-deficient mice. These results suggest that sulfated glycolipids may be involved in proliferation of OPCs in vivo.
authors.affiliation: Department of Molecular Neurobiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kajigaya
authors.forename: Kenji F
authors.initials: KF
authors.lastname: Tanaka
authors.forename: Akiko
authors.initials: A
authors.lastname: Hayashi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Suzuki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ishibashi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikenaka
authors.forename: Hiroko
authors.initials: H
authors.lastname: Baba
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D000941:Antigens / Q000378:metabolism; D001369:Axons / Q000378:metabolism / Q000473:pathology; D002452:Cell Count; D016923:Cell Death; D019070:Cell Lineage; D049109:Cell Proliferation; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018991:Myelin Proteolipid Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000378:metabolism; D009836:Oligodendroglia / Q000378:metabolism / Q000473:pathology*; D009900:Optic Nerve / Q000254:growth & development / Q000378:metabolism / Q000473:pathology*; D011509:Proteoglycans / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000378:metabolism; D013433:Sulfoglycosphingolipids / Q000378:metabolism; D015238:Sulfotransferases / Q000172:deficiency* / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D027341:Excitatory Amino Acid Transporter 1; D018991:Myelin Proteolipid Protein; D011509:Proteoglycans; D016186:Receptor, Macrophage Colony-Stimulating Factor; D013433:Sulfoglycosphingolipids; D015238:Sulfotransferases; D000099216:Chondroitin Sulfate Proteoglycan 4; C493393:Slc1a3 protein, mouse; C020556:galactosylceramide sulfotransferase