title: Crystal structure of prephenate dehydrogenase from Streptococcus mutans.
abstract: Prephenate dehydrogenase (PDH) is a bacterial enzyme that catalyzes conversion of prephenate to 4-hydroxyphenylpyruvate through the oxidative decarboxylation pathway for tyrosine biosynthesis. This enzymatic pathway exists in prokaryotes but is absent in mammals, indicating that it is a potential target for the development of new antibiotics. The crystal structure of PDH from Streptococcus mutans in a complex with NAD(+) shows that the enzyme exists as a homo-dimer, each monomer consisting of two domains, a modified nucleotide binding N-terminal domain and a helical prephenate C-terminal binding domain. The latter is the dimerization domain. A structural comparison of PDHs from mesophilic S. mutans and thermophilic Aquifex aeolicus showed differences in the long loop between β6 and β7, which may be a reason for the high K(m) values of PDH from Streptococcus mutans.
authors.affiliation: Division of Metrology for Quality of Life, Department of Bio-Analytical Science, University of Science & Technology, Daejeon, Republic of Korea.
authors.forename: Hyung-Keun
authors.initials: HK
authors.lastname: Ku
authors.forename: Nam Hyuk
authors.initials: NH
authors.lastname: Do
authors.forename: Jin Sue
authors.initials: JS
authors.lastname: Song
authors.forename: Saehae
authors.initials: S
authors.lastname: Choi
authors.forename: Seung Hoon
authors.initials: SH
authors.lastname: Yeon
authors.forename: Min Hyung
authors.initials: MH
authors.lastname: Shin
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Sang-Ryoul
authors.initials: SR
authors.lastname: Park
authors.forename: Il-young
authors.initials: IY
authors.lastname: Park
authors.forename: Sook-Kyung
authors.initials: SK
authors.lastname: Kim
authors.forename: Soo Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003509:Cyclohexanecarboxylic Acids / Q000737:chemistry / Q000378:metabolism; D053138:Cyclohexenes / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D011303:Prephenate Dehydrogenase / Q000737:chemistry*; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology*
chemical_list: D000900:Anti-Bacterial Agents; D003509:Cyclohexanecarboxylic Acids; D053138:Cyclohexenes; D009243:NAD; D011303:Prephenate Dehydrogenase; C005550:prephenic acid