title: [The effect of Uygur medicine Hyssopus officinalis L on expression of T-bet, GATA-3 and STAT-3 mRNA in lung tissue of asthma rats].
abstract: OBJECTIVE
  To study the Uygur medicine Hyssopus officinalis L on T-bet, GATA-3, STAT-3 mRNA levels of asthma rats in order to explore the mechanism of its treatment of asthma.
  
  
  METHODS
  Rats were randomly divided into normal control group, asthma model group and dexamethasone group and water extract of Hyssopus officinalis L high and low dose group. The rats were sensitized with OVA, Al(OH)(3); and DPT vaccine and then challenged with inhalation of aerosolized OVA solution for Preparation of asthma model and the level of T-bet, GATA-3, STAT-3 mRNA were detected with RT-PCR.
  
  
  RESULTS
  The normal control group and model group, model group and treatment group, the expression of T-bet, GATA-3 and STAT-3 mRNA in the lung tissue was statistically significant differences(P<0.05). Compared with model group, after treatment of Hyssopus officinalis L the expression of GATA-3 and STAT-3 mRNA of asthma rats significantly reduced (P<0.05), but the expression of T-bet mRNA was significantly higher (P<0.05).The expression of GATA-3 and STAT-3 mRNA of Hyssopus officinalis L high-dose treatment group was lower than the low-dose treatment group (P<0.05), but T-bet mRNA that was higher(P<0.05). The expression of T-bet mRNA has negative correlation with GATA-3 mRNA (r=-0.696), the expression of STAT-3 mRNA has correlation with T-bet mRNA and GATA-3 mRNA(r=-0.767, 0.772), P<0.05.
  
  
  CONCLUSIONS
  Hyssopus officinalis L probably regulates the differentiation of Th1, Th2 and Th17 on transcription level to play the role of anti-inflammatory.
authors.affiliation: Xinjiang VIP Lab of Great Physician and Pharmacology of Traditional Chinese Medical Formulae, Urumqi, China.
authors.forename: Hong-ying
authors.initials: HY
authors.lastname: Wang
authors.forename: Jian-bing
authors.initials: JB
authors.lastname: Ding
authors.forename: Upur
authors.initials: U
authors.lastname: Halmurat
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.forename: Zhi-qin
authors.initials: ZQ
authors.lastname: Xue
authors.forename: Ming
authors.initials: M
authors.lastname: Zhu
authors.forename: Shu-ge
authors.initials: SG
authors.lastname: Tian
authors.forename: Xiu-min
authors.initials: XM
authors.lastname: Ma
mesh_terms: D000818:Animals; D001249:Asthma / Q000473:pathology*; D004195:Disease Models, Animal; D050990:GATA3 Transcription Factor / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D019686:Lamiaceae / Q000737:chemistry*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D028321:Plant Preparations / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor / Q000235:genetics; D020825:T-Box Domain Proteins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; D028321:Plant Preparations; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D014157:Transcription Factors