title: Evaluation of microbiological air quality and of microclimate in university classrooms.
abstract: The proliferation of air-diffused microorganisms inside public buildings such as schools, hospitals, and universities, is often indicated as a possible health risk. In this research, we have illustrated the results of an investigation realized to determine the health of the air in some university classrooms, both from a microbiological and a microclimatic viewpoint, during the normal didactic activity of direct lessons. The results obtained have been expressed by means of contamination indices, already used in previous works. Very little contamination was recorded in the different phases of air treatment, which underlines the efficiency of the system and of the maintenance protocols. The Global Index of Microbial Contamination (GIMC per cubic meter) showed a value greater than the mean during the heating period (290), while the highest values (95th percentile 1,138.45) were recorded in the period using air conditioning. The index of mesophilic bacterial contamination, though it did not show any significant differences in the various modes of air treatment, showed a mean value (1.34) and the 95th percentile value (4.14), which was greater in the air-conditioning phase. Finally, the mean value of the amplification index underlined a decrease in the microbial contamination in comparison to the outside, while showing situations of increased microbial amplification during the period of simple ventilation (95th percentile 4.27). The 95th percentile values found for GICM in the three sampling periods, however, permitted us to identify the value of GIMC per cubic meter equal to 1,000 as a guide to provide a means of self-monitoring the quality of the air inside the classrooms. From a microclimatic viewpoint, two periods of the year manifested discomfort situations: during the heating phase (winter) and during the simple ventilation phase (spring). The results obtained indicate, therefore, a need to intervene on the environmental parameters, not being able, in this particular case, to intervene on other aspects that influence the microclimate.
authors.affiliation: Department of Drug Sciences, Laboratory of Microbiology, University of Pavia, Pavia, Italy. pietro.grisoli@unipv.it
authors.forename: Pietro
authors.initials: P
authors.lastname: Grisoli
authors.forename: Marinella
authors.initials: M
authors.lastname: Rodolfi
authors.forename: Tiziana
authors.initials: T
authors.lastname: Chiara
authors.forename: Laura Attinia
authors.initials: LA
authors.lastname: Zonta
authors.forename: Cesare
authors.initials: C
authors.lastname: Dacarro
mesh_terms: D000389:Air Conditioning / Q000706:statistics & numerical data; D000391:Air Microbiology; D016902:Air Pollution, Indoor / Q000032:analysis* / Q000706:statistics & numerical data; D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification; D004784:Environmental Monitoring; D005658:Fungi / Q000254:growth & development / Q000302:isolation & purification; D008834:Microclimate; D014495:Universities; D014691:Ventilation / Q000706:statistics & numerical data