title: The myosin-related motor protein Myo2 is an essential mediator of bud-directed mitochondrial movement in yeast.
abstract: The inheritance of mitochondria in yeast depends on bud-directed transport along actin filaments. It is a matter of debate whether anterograde mitochondrial movement is mediated by the myosin-related motor protein Myo2 or by motor-independent mechanisms. We show that mutations in the Myo2 cargo binding domain impair entry of mitochondria into the bud and are synthetically lethal with deletion of the YPT11 gene encoding a rab-type guanosine triphosphatase. Mitochondrial distribution defects and synthetic lethality were rescued by a mitochondria-specific Myo2 variant that carries a mitochondrial outer membrane anchor. Furthermore, immunoelectron microscopy revealed Myo2 on isolated mitochondria. Thus, Myo2 is an essential and direct mediator of bud-directed mitochondrial movement in yeast. Accumulating genetic evidence suggests that maintenance of mitochondrial morphology, Ypt11, and retention of mitochondria in the bud contribute to Myo2-dependent inheritance of mitochondria.
authors.affiliation: Institut für Zellbiologie, Universität Bayreuth, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Förtsch
authors.forename: Eric
authors.initials: E
authors.lastname: Hummel
authors.forename: Melanie
authors.initials: M
authors.lastname: Krist
authors.forename: Benedikt
authors.initials: B
authors.lastname: Westermann
mesh_terms: D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000378:metabolism*; D009154:Mutation; D018995:Myosin Heavy Chains / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D024701:Myosin Type V / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C077512:MYO2 protein, S cerevisiae; D033681:Mitochondrial Membrane Transport Proteins; D020409:Molecular Motor Proteins; D029701:Saccharomyces cerevisiae Proteins; C468556:Ypt11 protein, S cerevisiae; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins; D020691:rab GTP-Binding Proteins