title: [Regulation of MAVS-mediated pathway by p21].
abstract: OBJECTIVE
  To study the interaction between MAVS and p21 regulates MAVS-mediated pathway.
  
  
  METHODS
  Firstly, we cloned full-length p21 gene by PCR. And then the interaction between MAVS and p21 was further demonstrated by co-immunoprecipitation. The regulation of MAVS-mediated pathway by p21 was analyzed by luciferase reporter assays.
  
  
  RESULTS
  There are interaction between p21 and MAVS in 293T cells. p21 downregulates activity of MAVS.
  
  
  CONCLUSIONS
  It is shown that p21 inhibits MAVS-mediated activation of NF-κB and IFN-β by Luciferase reporter assays. Overall, these results laid the foundation for further investigating the function of p21 in MAVS-mediated pathway.
authors.affiliation: Laboratory Medical College, Beihua University, Jilin , China.
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Wen-li
authors.initials: WL
authors.lastname: Su
authors.forename: Ji-wei
authors.initials: JW
authors.lastname: Bai
authors.forename: Yan
authors.initials: Y
authors.lastname: Ling
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Yong-mei
authors.initials: YM
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: He
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D011485:Protein Binding / Q000502:physiology; D015398:Signal Transduction / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D050759:Cyclin-Dependent Kinase Inhibitor p21; C505371:MAVS protein, human