title: Comparative gastroprotective effect of post-treatment with low doses of rutin and cimetidine in rats.
abstract: The anti-ulcerogenic potentials of low doses of rutin and cimetidine in ethanol-, acetic acid-, and stress-induced ulcers in rats have been evaluated and compared in this study. In each model, male Wistar rats were randomly divided into six groups (I-VI). Groups II-VI were administered 1 mL/100 g ethanol orally, 0.05 mL of 20% acetic acid submucosally or kept in a cold chamber for 6 h to induce ulcer in the ethanol-, acetic acid-, and stress-induced ulceration model, respectively. Thereafter, group III was post-treated with 300 mg/kg cimetidine and groups IV-VI with 20, 40, and 80 mg/kg rutin, respectively, while the control (group I) received distilled water in Tween 20. One hour after post-treatment, all groups were killed and the gastric ulcer index was calculated. Malondialdehyde (MDA) level, vitamin C content, and glutathione peroxidase (GPx) activity were evaluated in the gastric mucosa of animals. Post-treatment with rutin significantly reduced ulcerogen-induced gastric damage in all models. This effect was significant at all dose levels compared with the ulcer-induced groups. Rutin significantly reduced the MDA levels but increased the vitamin C content and GPx activity. Ulcer index and MDA level were highest in the ethanol-induced ulcer model while vitamin C content and GPx activity were lowest in the stress-induced ulcer model. The study showed that all three models of ulceration appeared to be linked to oxidative stress and also ascribed significant anti-ulcerogenic potential to rutin especially at lower doses of 20-80 mg/kg.
authors.affiliation: Department of Biochemistry, Federal University of Technology, PMB 704, Akure, Nigeria.
authors.forename: Mary Tolulope
authors.initials: MT
authors.lastname: Olaleye
authors.forename: Afolabi Clement
authors.initials: AC
authors.lastname: Akinmoladun
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D008315:Malondialdehyde; D012431:Rutin; D002927:Cimetidine; D005979:Glutathione Peroxidase; D001205:Ascorbic Acid