title: Conventional and microwave-assisted SPPS approach: a comparative synthesis of PTHrP(1-34)NH(2).
abstract: Attracted by the possibility to optimize time and yield of the synthesis of difficult peptide sequences by MW irradiation, we compared Fmoc/tBu MW-assisted SPPS of 1-34 N-terminal fragment of parathyroid hormone-related peptide (PTHrP) with its conventional SPPS carried out at RT. MWs were applied in both coupling and deprotection steps of SPPS protocol. During the stepwise elongation of the resin-bound peptide, monitoring was conducted by performing MW-assisted mini-cleavages and analyzing them by UPLC-ESI-MS. Identification of some deletion sequences was helpful to recognize critical couplings and as such helped to guide the introduction of MW irradiations to these stages.
authors.affiliation: Department of Chemistry Ugo Schiff, Laboratory of Peptide & Protein Chemistry & Biology, Via della Lastruccia 3/13, Polo Scientifico e Tecnologico, University of Florence, I-50019 Sesto Fiorentino, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Rizzolo
authors.forename: Chiara
authors.initials: C
authors.lastname: Testa
authors.forename: Duccio
authors.initials: D
authors.lastname: Lambardi
authors.forename: Michael
authors.initials: M
authors.lastname: Chorev
authors.forename: Mario
authors.initials: M
authors.lastname: Chelli
authors.forename: Paolo
authors.initials: P
authors.lastname: Rovero
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Papini
mesh_terms: D008872:Microwaves; D044162:Parathyroid Hormone-Related Protein / Q000138:chemical synthesis*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D044162:Parathyroid Hormone-Related Protein