title: Chemopreventive efficacy of geraniol against 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis.
abstract: The status of lipid peroxidation, antioxidants, and detoxification enzymes were used as biochemical end points to assess the chemopreventive potential of geraniol, a monoterpene, in 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Topical application of 0.5% DMBA in liquid paraffin, three times a week, for 14 weeks developed well-differentiated squamous cell carcinoma in the buccal pouch of golden Syrian hamsters. Although 100% tumor formation was noticed in hamsters treated with DMBA alone, intragastric administration of geraniol, at a dose of 250 mg/kg body weight (b.w.) to DMBA-treated hamster completely prevented the formation of oral tumors. Furthermore, geraniol significantly reduced lipid peroxidation by-products and improved the status of enzymatic and non-enzymatic antioxidants as well as modulated the status of phase I and phase II detoxification enzymes, favoring the excretion of carcinogenic metabolite, during DMBA-induced oral carcinogenesis. The present study concludes that the chemopreventive potential of geraniol relies on its anti-lipid peroxidative and antioxidant function as well as modulatory effects on phase I and II detoxification enzymes to excrete the carcinogenic metabolite, during DMBA-induced hamster buccal pouch carcinogenesis.
authors.affiliation: Department of Biochemistry & Biotechnology, Annamalai University, Tamil Nadu, India.
authors.forename: Veerasamy
authors.initials: V
authors.lastname: Vinothkumar
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Manoharan
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000633:toxicity; D000080462:Acyclic Monoterpenes; D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D006224:Cricetinae; D005982:Glutathione Transferase / Q000378:metabolism; D008658:Inactivation, Metabolic; D015227:Lipid Peroxidation; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000378:metabolism / Q000473:pathology*; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D013729:Terpenes / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000080462:Acyclic Monoterpenes; D016588:Anticarcinogenic Agents; D000975:Antioxidants; D013729:Terpenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; C007836:geraniol