title: Characterization of the effective cellulose degrading strain CTL-6.
abstract: An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase (CMCase activity) was 0.404 micromol/(min mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65 degrees C with the highest degradation efficiency observed at 60 degrees C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so.
authors.affiliation: Alan G. MacDiarmid Research Institute of Renewable Energy, China Three Gorges University, Yichang 443002, China. Lv_yucai@hotmail.com
authors.forename: Yucai
authors.initials: Y
authors.lastname: Lü
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ishii
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Igarashi
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D058645:Denaturing Gradient Gel Electrophoresis; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D010209:Paper; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D002482:Cellulose; D002480:Cellulase; C019826:carboxymethylcellulase