title: Optimization of the biomass production of oil algae Chlorella minutissima UTEX2341.
abstract: High production cost is a major obstacle to the extensive use of microalgae biodiesel. To cut the cost and achieve higher biomass productivity, Chlorella minutissima UTEX2341 was cultured under photoheterotrophic conditions. With the carbon, nitrogen and phosphorus concentration of 26.37, 2.61 and 0.03 g L⁻¹ d⁻¹ respectively, a maximum biomass productivity of 1.78 g L⁻¹ d⁻¹ was obtained, which was 59 times more than that cultured under autotrophic condition. The lipid productivity reached 0.29 g L⁻¹ d⁻¹, which was 11.9 times higher than the highest value reported by Oh et al. (2010). The conversion rate of microalgae lipids to FAME was found to be elevated from 45.65% to 62.97% and the FAME productivity increased from 1.16 to 180.68 mg L⁻¹ d⁻¹ after the optimization. 94% of the fatty acid of C. minutissima UTEX2341 was found to be composed of palmitic, oleic, linoleic and γ linoleic and the unsaturated fatty acids were the main parts (79.42%).
authors.affiliation: State Key Laboratory of Agro-Biotechnology, College of Biological Sciences, China Agricultural University, No. 2, Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
authors.forename: ZhaoSheng
authors.initials: Z
authors.lastname: Li
authors.forename: HongLi
authors.initials: H
authors.lastname: Yuan
authors.forename: JinShui
authors.initials: J
authors.lastname: Yang
authors.forename: BaoZhen
authors.initials: B
authors.lastname: Li
mesh_terms: D056804:Biofuels; D018533:Biomass; D002244:Carbon / Q000378:metabolism; D002364:Caseins / Q000737:chemistry; D002708:Chlorella / Q000254:growth & development*; D002849:Chromatography, Gas; D003470:Culture Media / Q000737:chemistry*; D005227:Fatty Acids / Q000096:biosynthesis*; D005990:Glycerol / Q000737:chemistry; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism
chemical_list: D056804:Biofuels; D002364:Caseins; D003470:Culture Media; D005227:Fatty Acids; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen; D005990:Glycerol