title: Animal models of mycobacteria infection.
abstract: This unit describes the infection of mice and guinea pigs with mycobacteria via various routes, as well as necropsy methods for the determination of mycobacterial loads within target organs. Additionally, methods for cultivating mycobacteria and preparing stocks are described. The protocols outlined are primarily used for M. tuberculosis, but can also be used for the study of other non-tuberculosis mycobacterial species.
authors.affiliation: Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA.
authors.forename: Diane J
authors.initials: DJ
authors.lastname: Ordway
authors.forename: Ian M
authors.initials: IM
authors.lastname: Orme
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004195:Disease Models, Animal; D018450:Disease Progression; D006168:Guinea Pigs; D006801:Humans; D007109:Immunity; D051379:Mice; D009161:Mycobacterium / Q000276:immunology* / Q000472:pathogenicity; D009164:Mycobacterium Infections / Q000276:immunology* / Q000503:physiopathology