title: [Study on HLA nucleotide sequence matching in epitope positions among recipient-donor pairs for allogenic hematopoietic stem-cell transplantation].
abstract: OBJECTIVE
  To analyze the human leukocyte antigens(HLA)-A, -B, -Cw, -DRB1 and DQB1 nucleotide sequences between patients waiting for allogenic hematopoietic stem-cell transplantation (HSCT) and donors in Chinese population, and to establish strategy for maximizing optimal donor selection.
  
  
  METHODS
  HLA high-resolution typing in a total of 537 recipient-donor pairs was determined by sequence based typing (SBT) method. The nucleotide BLAST tool was used to compare the nucleotide sequences among recipient-donor pairs.
  
  
  RESULTS
  Only 16.20% (88/537) of recipient-donor pairs were found to fully match for nucleotide sequences of all HLA-A,-B,-Cw, -DRB1 and -DQB1 loci. Mismatch rate in single locus were 8.38% in HLA-A, 0.74% in HLA-B, 12.29% in HLA-C, 2.42% in HLA-DRB1, and 2.79% in HLA-DQB1, respectively. Mismatch rate in two or multiple HLA loci was 42.65%. Nonpermissive allele mismatch combinations (A 02:01-A 02:06, A 02:06-A 02:07, Cw 03:04-Cw 15:02, Cw 03:03-Cw 04:01, Cw 03:04-Cw 14:02, Cw 03:03-Cw 08:01, DRB1 04:03:01-DRB1 04:05) were detected in single mismatch HLA locus of recipient-donor pairs, mismatches of B 07:05:01-B 07:06, Cw 07:01:01-Cw 07:06 combinations outside of epitope positions were detected in two recipient-donor pairs.
  
  
  CONCLUSIONS
  Our data suggested that attention should be paid in comparing nucleotide sequences between recipient and donor, and in distinguishing nucleotide sequence mismatches within and outside of the epitope positions. These results could serve as guidelines for donor selection.
authors.affiliation: Institute of Shenzhen Blood Center, Shenzhen HLA Laboratory of Chinese Marrow Donor Program, Shenzhen, Guangdong 518035, P. R. China.
authors.forename: Su-qing
authors.initials: SQ
authors.lastname: Gao
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Zou
authors.forename: Shi-zheng
authors.initials: SZ
authors.lastname: Jing
authors.forename: Liang-hong
authors.initials: LH
authors.lastname: Cheng
authors.forename: Tian-li
authors.initials: TL
authors.lastname: Wei
authors.forename: Da-ming
authors.initials: DM
authors.lastname: Wang
authors.forename: Liu-mei
authors.initials: LM
authors.lastname: He
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Deng
mesh_terms: D001483:Base Sequence; D046148:Donor Selection / Q000379:methods*; D000939:Epitopes / Q000235:genetics*; D006680:HLA Antigens / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D014019:Tissue Donors
chemical_list: D000939:Epitopes; D006680:HLA Antigens