title: Discovery of an orthoreovirus in the aborted fetus of a Steller sea lion (Eumetopias jubatus).
abstract: An aborted mid-gestational male Steller sea lion fetus with an attached placenta was recovered on the floor of an open floating capture trap located off Norris Rock near Denman Island, British Columbia. Viral culture of the placenta demonstrated cytopathic effect. Although no specific signal was obtained in microarray experiments using RNA obtained from viral culture, elution and sequence analysis revealed the presence of a reovirus. Complete genome pyrosequencing led to the identification of an orthoreovirus that we have tentatively named Steller sea lion reovirus (SSRV). Phylogenetic analysis revealed similarities between SSRV and orthoreoviruses of birds, bats and other mammals that suggests potential for interspecies transmission.
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Palacios
authors.affiliation: Marine Animal Disease Laboratory, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: James F X
authors.initials: JFX
authors.lastname: Wellehan
authors.affiliation: Animal Health Center, Abbotsford Agriculture Centre, Provincial Government of British Columbia, Abbotsford, British Columbia, Canada.
authors.forename: Stephen
authors.initials: S
authors.lastname: Raverty
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Ana V
authors.initials: AV
authors.lastname: Bussetti
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hui
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: Nazir
authors.initials: N
authors.lastname: Savji
authors.affiliation: Marine Animal Disease Laboratory, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
authors.forename: Hendrik H
authors.initials: HH
authors.lastname: Nollens
authors.affiliation: Marine Mammal Investigations, Washington Department of Fish and Wildlife, Washington, USA.
authors.forename: Dyanna
authors.initials: D
authors.lastname: Lambourn
authors.affiliation: 454 Life Sciences, Branford, CT, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Celone
authors.affiliation: 454 Life Sciences, Branford, CT, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Hutchison
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Calisher
authors.affiliation: Department of Fisheries and Oceans Canada, Winnipeg, Manitoba, Canada.
authors.forename: Ole
authors.initials: O
authors.lastname: Nielsen
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D037881:Aborted Fetus / Q000821:virology*; D000818:Animals; D001955:British Columbia; D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D008297:Male; D008969:Molecular Sequence Data; D018165:Orthoreovirus / Q000302:isolation & purification*; D010802:Phylogeny; D010920:Placenta / Q000821:virology; D011247:Pregnancy; D012367:RNA, Viral / Q000235:genetics*; D046070:Sea Lions / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral