title: [The effects of adipose-derived stem cells transplantation on the expression of TGF-β1 in rat brain after cerebral ischemia].
abstract: OBJECTIVE
  To explore the mechanisms of adipose-derived stem cells (ADSCs) transplanting induced angiogenesis in rat brain after focal cerebral ischemia.
  
  
  METHODS
  108 male adult Sprague-Dawley rats were randomly assigned into 4 groups: sham-operated group, middle cerebral artery occlusion (MCAO) group, vehicle group and MCAO+ADSCs-treated group. Rat's focal cerebral ischemia model was established by right middle cerebral artery occlusion (MCAO) with modified Longa's method. ADSCs were pre-labeled by CFSE before the transplantation into the rat brain. At 1 d after MCAO, 30 μL cell suspension which contained 1×10(6); ADSCs was injected into the lateral ventricle of MCAO+ADSCs-treated rats, and the same dose of PBS was given to the rats of vehicle group as control. At 4 d, 7 d and 14 d after MCAO, rats were decapitated to detect the TGF-β1 expression in the infarct area.
  
  
  RESULTS
  The expression of TGF-β1 in brain tissues in MCAO+ADSCs-treated group was significantly higher than MCAO group and vehicle group at 4 d, 7 d and 14 d after MCAO, respectively. After transplantation into MCAO rats, ADSCs could survive and express TGF-β1 in the ischemic brain.
  
  
  CONCLUSIONS
  These data suggest that ADSCs transplantation can promote revascularization in cerebral ischemic rats, partly by promoting TGF-β1 expression in the brain.
authors.affiliation: Department of Neurology, Fujian Medical University, Fuzhou, China. hhuang469@gmail.com
authors.forename: Huan
authors.initials: H
authors.lastname: Huang
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Jie-hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Yi-xian
authors.initials: YX
authors.lastname: Zhang
authors.forename: Hou-wei
authors.initials: HW
authors.lastname: DU
authors.forename: Rong-hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Hua-pin
authors.initials: HP
authors.lastname: Huang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1