title: Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins.
abstract: Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).
authors.affiliation: Microbiological Diseases and Diagnostics Research, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia.
authors.forename: Sam
authors.initials: S
authors.lastname: Abraham
authors.forename: James
authors.initials: J
authors.lastname: Chin
authors.forename: Huub J M
authors.initials: HJ
authors.lastname: Brouwers
authors.forename: Bernadette
authors.initials: B
authors.lastname: Turner
authors.forename: Ren
authors.initials: R
authors.lastname: Zhang
authors.forename: Toni A
authors.initials: TA
authors.lastname: Chapman
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003087:Colicins / Q000633:toxicity*; D004249:DNA Damage / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013014:SOS Response, Genetics; D014158:Transcription, Genetic
chemical_list: D003087:Colicins; D049452:Green Fluorescent Proteins