title: Effect of ethanol extract of Sphaeranthus indicus on cisplatin-induced nephrotoxicity in rats.
abstract: Cisplatin is an anticancer drug extensively used against a variety of cancers. Cisplatin chemotherapy is found to manifest dose-dependent nephrotoxicity. Depletion of the renal antioxidant defence system has been suggested to be the main cause of cisplatin-induced nephrotoxicity. The purpose of this study is to investigate whether the ethanol extract of entire plant of Sphaeranthus indicus could reduce the intensity of toxicity in albino rats. Nephrotoxicity was assessed by determining the serum creatinine and urea levels and renal antioxidant status in rats after cisplatin administration (12 mg kg(-1) body weight, i.p.). The ethanol extract of S. indicus (150 and 300 mg kg(-1) body weight) was administered orally from the sixth day onwards for 10 days after cisplatin administration. The extract significantly reduced the elevated serum creatinine and urea levels. Renal antioxidant defence systems, such as superoxide dismutase, catalase, glutathione peroxidase activities and reduced glutathione level that are depleted by cisplatin therapy were restored to normal by treatment with the extract. Cisplatin-induced lipid peroxidation was also found to be markedly reduced by treatment with the extract. These results suggest that S. indicus has protective effect against cisplatin-induced nephrotoxicity, which may be attributed to its antioxidant potential.
authors.affiliation: Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, India.
authors.forename: Jessy Elizabeth
authors.initials: JE
authors.lastname: Mathew
authors.forename: Alex
authors.initials: A
authors.lastname: Joseph
authors.forename: Keloth
authors.initials: K
authors.lastname: Srinivasan
authors.forename: Seekarajapuram Vachala
authors.initials: SV
authors.lastname: Dinakaran
authors.forename: Anand
authors.initials: A
authors.lastname: Mantri
authors.forename: Vinit
authors.initials: V
authors.lastname: Movaliya
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D019659:Asteraceae / Q000737:chemistry*; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000431:Ethanol; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D002945:Cisplatin