title: Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus.
abstract: SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallographic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a=b=112.8, c=91.1 Å, α=β=90, γ=120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å3 Da(-1) and a solvent content of 56.2%.
authors.affiliation: National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Ren
authors.forename: Zehua
authors.initials: Z
authors.lastname: Bao
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ming
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D015870:Gene Expression; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D045473:Severe acute respiratory syndrome-related coronavirus / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D017361:Viral Nonstructural Proteins; C087633:nonstructural protein, coronavirus