title: Protection by 3'-methoxypuerarin of rat hippocampal neurons against ischemia/reperfusion injury.
abstract: 3'-Methoxypuerarin (3'-MOP) is an isoflavone extracted from radix puerariae. The aim of this study was to investigate the role and the mechanism of 3'-MOP in the protection of hippocampal neurons against cerebral ischemia/reperfusion (I/R) injury in rats. I/R injury was induced by a modified four-vessel occlusion model. Rats were randomly divided into an I/R group, an I/R + 3'-MOP group and a control group. Histological changes in the neurons of the hippocampal CA1 region were observed with hematoxylin and eosine (H&E) staining. The apoptotic neurons in the hippocampal CA1 area were counted with the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining. The results showed that compared with the I/R group, 3'-MOP increased the number of surviving neurons in the hippocampal CA1 region (P < 0.001) and markedly reduced the number of apoptotic pyramidal neurons (P < 0.001) after I/R injury. In conclusion, 3'-MOP can protect hippocampal neurons against I/R injury by inhibiting apoptosis.
authors.affiliation: Institute of Physiology, School of Medicine, Shandong University, Jinan 250012, Shandong, People's Republic of China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
authors.forename: Kejing
authors.initials: K
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chai
authors.forename: Jingxin
authors.initials: J
authors.lastname: Li
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.forename: Lianbi
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology*; D007274:Injections, Intraperitoneal; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: C517305:3'-methoxypuerarin; D007529:Isoflavones