title: Detection of gene expression in mouse embryos and tissue sections.
abstract: Analysis of gene expression patterns is central to the study of embryonic development. This chapter details methods for detecting gene expression in whole mouse embryos and in tissue sections. The most commonly used methods available in mouse are described and include mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA, USA. emcglinn@receptor.med.harvard.edu
authors.forename: Edwina
authors.initials: E
authors.lastname: McGlinn
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Mansfield
mesh_terms: D000818:Animals; D016616:Cryoultramicrotomy; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D016591:Histocytological Preparation Techniques / Q000379:methods*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D010232:Paraffin / Q000378:metabolism; D015347:RNA Probes / Q000235:genetics
chemical_list: D015347:RNA Probes; D010232:Paraffin; D005780:Gelatin