title: Co-delivery of adenovirus and carmustine by anionic liposomes with synergistic anti-tumor effects.
abstract: OBJECTIVE
  To improve gene transducibility mediated by adenovirus (Ad) in cancer cells and further enhance anti-tumor effects by co-delivery.
  
  
  METHODS
  Calcium-induced phase change method was used to prepare the complex of anionic liposomes and adenovirus (AL/Ad5). Gene expression was qualitatively detected by X-gal staining and quantitatively detected by ELISA. Taking adenovirus-mediated stromal cell-derived factor-1α (Ad5-SDF1α) as therapeutic gene and carmustine (BCNU) as chemotherapeutic agent, a co-delivering system of AL/Ad5-SDF1α/BCNU was prepared and administered to tumor-bearing mice by intratumor injection.
  
  
  RESULTS
  Enhanced LacZ gene transduction was obtained in B16 and Lewis lung carcinoma cells in vitro and in vivo. Complexes of AL/Ad5-SDF1α improved SDF1α gene expression and led to accumulation of dendritic cells among the murine B16 melanoma cells in vivo. This co-delivery system of AL/Ad5-SDF1α/BCNU could significantly suppress tumor growth and prolong survival of tumor-bearing mice.
  
  
  CONCLUSIONS
  Through the co-delivering system, AL/Ad5-SDF1α could synergize with BCNU to improve the antitumor effect. It may be a promising strategy for solid tumor therapy.
authors.affiliation: Luzhou Medical College, Luzhou, Sichuan, China, 646000.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhong
authors.forename: Yu
authors.initials: Y
authors.lastname: Wan
authors.forename: Sanjun
authors.initials: S
authors.lastname: Shi
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Han
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Sun
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D018906:Antineoplastic Agents, Alkylating / Q000008:administration & dosage*; D002330:Carmustine / Q000008:administration & dosage*; D054377:Chemokine CXCL12 / Q000235:genetics*; D004357:Drug Synergism; D005697:Galactosides / Q000032:analysis; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage*; D007211:Indoles / Q000032:analysis; D015552:Injections, Intralesional; D007763:Lac Operon; D008081:Liposomes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000628:therapy*; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D018906:Antineoplastic Agents, Alkylating; D054377:Chemokine CXCL12; D005697:Galactosides; D007211:Indoles; D008081:Liposomes; D002330:Carmustine; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside