title: Evaluation of the aflatoxin biosynthetic genes for identification of the Aspergillus section Flavi.
abstract: Several fungi in the Aspergillus section Flavi have been widely used for fermentative food production, while some related species in the section are known to produce mycotoxin(s) that causes mycotic diseases. Common evolutionary markers, such as rRNA gene sequences and their internal transcribed spacers, cannot differentiate these non-aflatoxin-producing species from aflatoxin producers. Multilocus sequence analysis (MLSA) based on four aflatoxin biosynthetic genes encoding aflR, aflT, norA, and vbs, which are more variable nucleotide sequences than rRNA genes, can distinguish safe koji molds, A. oryzae and A. sojae, from aflatoxin-producing strains, A. flavus, A. toxicarius and A. parasiticus.
authors.affiliation: International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Nakamura
authors.forename: Takashi
authors.initials: T
authors.lastname: Narihiro
authors.forename: Naoki
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: Hanako
authors.initials: H
authors.lastname: Mochimaru
authors.forename: Rena
authors.initials: R
authors.lastname: Matsumoto
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Tanabe
authors.forename: Keiko
authors.initials: K
authors.lastname: Hagiya
authors.forename: Kiriko
authors.initials: K
authors.lastname: Ikeba
authors.forename: Akihiko
authors.initials: A
authors.lastname: Maruyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hanada
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics*; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal; D005838:Genotype; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D000348:Aflatoxins; D004271:DNA, Fungal