title: Myocardial oxidative stress following sub-chronic and chronic oral cadmium exposure in rats.
abstract: We investigated the effect of oral cadmium intoxication on the antioxidant/prooxidant status in serum and heart. Wistar rats, separated into four groups, that received: (1) tap water for 60 days (control); (2), (3) and (4) Cd(2+) (15 ppm)-containing water, during 15, 30 and 60 days, respectively. Lipoperoxidation increased in serum and heart of group 4. Circulating paraoxonase-1 activity was higher in groups 2 and 3. Protein carbonyl-groups increased while total and reduced glutathione levels decreased in the heart after 15 days of cadmium intoxication. Cardiac catalase activity was higher in groups 3 and 4 but glutathione peroxidase activity diminished in the heart of all poisoned groups. Superoxide dismutase transcript levels as well as Nrf2 expression also increased in the heart of groups 2 and 3, while gp91phox and p47phox mRNA levels rose only in group 3. We suggest cadmium intoxication modifies antioxidant/prooxidant ratio in serum and heart in a time-of-exposure-dependent way.
authors.affiliation: Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, IMIBIO-SL, CONICET, San Luis, Argentina.
authors.forename: Mariana Lucila
authors.initials: ML
authors.lastname: Ferramola
authors.forename: Rosa Isabel
authors.initials: RI
authors.lastname: Antón
authors.forename: Ana Cecilia
authors.initials: AC
authors.lastname: Anzulovich
authors.forename: María Sofía
authors.initials: MS
authors.lastname: Giménez
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D002104:Cadmium / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D004785:Environmental Pollutants / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000378:metabolism; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D059551:Toxicity Tests, Subacute
chemical_list: D004785:Environmental Pollutants; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D017392:Thiobarbituric Acid Reactive Substances; D002104:Cadmium; D010088:Oxidoreductases; D043303:Aryldialkylphosphatase; D005978:Glutathione