title: Alpha-tocopherol ameliorates cypermethrin-induced toxicity and oxidative stress in the nematode Caenorhabdtis elegans.
abstract: Oxidative stress and other effects induced by cypermethrin (CYP, 15 mM) and their amelioration by alpha-tocopherol (400 microM) was studied in the nematode Caenorhabditis elegans. The worms exposed for 4 h to CYP showed increased levels of reactive oxygen species (46%), H2O2 (37%) and protein carbonyls (29%), accompanied by decreased lifespan and brood size. However, exposure to both CYP and alpha-tocopherol resulted in diminution of above alterations with the worms exhibiting relatively lower levels of ROS (30%), H2O2 (15%), protein carbonyls (14%), altered antioxidant enzyme activities and normal lifespan and brood size. The results suggest that CYP induces oxidative stress in C. elegans and the strategy of intervention with alpha-tocopherol could be exploited to offset this induced oxidative stress.
authors.affiliation: Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CFTRI, CSIR), Mysore 570 020, India.
authors.forename: Shivaiah
authors.initials: S
authors.lastname: Shashikumar
authors.forename: P S
authors.initials: PS
authors.lastname: Rajini
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007306:Insecticides; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D011722:Pyrethrins / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007306:Insecticides; D011722:Pyrethrins; D017382:Reactive Oxygen Species; C017160:cypermethrin; D006861:Hydrogen Peroxide; D024502:alpha-Tocopherol