title: Dielectric microsphere mediated transfection using a femtosecond laser.
abstract: We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000 nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800 nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1.06 J/cm(2). The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.
authors.affiliation: Department of Electronics and Electrical Engineering, Keio University, 3-14-1 Hiyoshi, Yokohama, Kanagawa 223-8522, Japan. terakawa@elec.keio.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Terakawa
authors.forename: Yuto
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001692:Biological Transport; D002463:Cell Membrane Permeability; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism; D017097:Electric Impedance; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D007834:Lasers; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D000906:Antibodies; D003911:Dextrans; D011137:Polystyrenes; D034741:RNA, Small Interfering; D016650:Fluorescein-5-isothiocyanate