title: Determination of active substances in binary mixture antiparasitic veterinary formulations by HPLC.
abstract: The purpose of the study was to develop a simple, versatile HPLC method for the identification and quantification of praziquantel and ivermectin (in Equimax) or praziquantel and abamectin (in Abamitel Plus). A satisfactory separation was obtained using the Supelcosil LC-ABZ+ column in gradient system with a mobile phase A: acetonitrile / water in 40:60 ratio and phase B: acetonitrile. The UV detection was set at 245 nm. The correlation coefficient values (> or = 0,998) for all active substances confirmed that the calibration curves (peak area vs. concentration) are linear. The results of the quantification and the statistical evaluation confirmed that the method is accurate and precise. It can also be applied to confirm the identity of benzyl alcohol, methyl p-hydroxybenzoate and propyl p-hydroxybenzoate in Abamitel Plus formulation.
authors.affiliation: Department of Basic and Applied Pharmacy, National Medicines Institute, 30/34 Chełmska St., 00-725 Warszawa, Poland. akulik@il.waw.pl
authors.forename: Anna
authors.initials: A
authors.lastname: Kulik
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szczotkowska
authors.forename: Wanda
authors.initials: W
authors.lastname: Białecka
authors.forename: Marzena
authors.initials: M
authors.lastname: Podolska
authors.forename: Barbara
authors.initials: B
authors.lastname: Kwiatkowska-Puchniarz
authors.forename: Aleksander
authors.initials: A
authors.lastname: Mazurek
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000977:Antiparasitic Agents / Q000032:analysis*; D019905:Benzyl Alcohol / Q000032:analysis; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D010226:Parabens / Q000032:analysis; D011223:Praziquantel / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D019155:Veterinary Drugs / Q000032:analysis*; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000977:Antiparasitic Agents; D004338:Drug Combinations; D010226:Parabens; D012997:Solvents; D019155:Veterinary Drugs; D014867:Water; C048324:abamectin; D011223:Praziquantel; D007559:Ivermectin; C015358:methylparaben; D019905:Benzyl Alcohol; C032159:acetonitrile; C006068:propylparaben