title: Direct observation of single RNA polymerase processing through a single endogenous gene in a living yeast cell.
abstract: Rapid advances in live-cell imaging have now enabled direct observation of the transcription of single nascent mRNA molecules from an endogenous yeast gene. A novel quantitative fluctuation analysis of fluorescently labeled mRNA revealed the kinetics of transcription initiation and the dynamics of elongation and termination.
authors.affiliation: Ludwig-Maximilians-Universität München, Department Chemie, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Treutlein
authors.forename: Jens
authors.initials: J
authors.lastname: Michaelis
mesh_terms: D005800:Genes, Fungal / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D012319:RNA Polymerase II