title: Rapid determination of fumagillin residues in honey by liquid chromatography-tandem mass spectrometry using the QuEChERS method.
abstract: A new, rapid, and efficient method for determining the fumagillin residues in honey was developed. The samples extracted were analyzed using LC/MS/MS. Chromatographic separation of fumagillin was performed in gradient mode on a C8 column (100 x 2.0 mm, 5 microm) at 40 degrees C. The mobile phase consisted of a mixture of 2 mM ammonium formate-0.01% formic acid solution and methanol; the flow rate was set to 0.2 mL/min. Under these conditions, it was possible to measure fumagillin and its isomers as a single peak. The sample preparation procedure used is based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) method, which is fast (approximately 30 min) and uses less organic solvent. The fumagillin was extracted with acetonitrile containing 0.1% formic acid, then purified using a solid-phase extraction method with an Oasis mixed-mode weak anion-exchange cartridge. The overall recovery of fumagillin ranged from 88.1 to 99.4%; the intra- and interassay CVs were <4.5% and <4.9%, respectively. The LOQ was 0.1 microg/kg. LC/MS/MS coupled with the QuEChERS method showed strong potential as a method for determining fumagillin residues in honey.
authors.affiliation: Tokyo Metropolitan Institute of Public Health, 3-24-1, Hyakunin-cho, Shinjuku-ku, Tokyo 169-0073, Japan. Maki_l_Kanda@member.metro.tokyo.jp
authors.forename: Maki
authors.initials: M
authors.lastname: Kanda
authors.forename: Takeo
authors.initials: T
authors.lastname: Sasamoto
authors.forename: Kazue
authors.initials: K
authors.lastname: Takeba
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kusano
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsushima
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakajima
authors.forename: Setsuko
authors.initials: S
authors.lastname: Kanai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takano
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005506:Food Contamination; D006722:Honey / Q000032:analysis*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003510:Cyclohexanes; D005231:Fatty Acids, Unsaturated; D012717:Sesquiterpenes; C026211:fumagillin