title: Prevalence of anaerobic ammonium-oxidizing bacteria in contaminated groundwater.
abstract: Anaerobic ammonium-oxidizing (anammox) bacteria perform an important step in the global nitrogen cycle: anaerobic oxidation of ammonium and reduction of nitrite to form dinitrogen gas (N(2)). Anammox organisms appear to be widely distributed in natural and artificial environments. However, their roles in groundwater ammonium attenuation remain unclear and only limited biomarker-based data confirmed their presence prior to this study. We used complementary molecular and isotope-based methods to assess anammox diversity and activity occurring at three ammonium-contaminated groundwater sites: quantitative PCR, denaturing gradient gel electrophoresis, sequencing of 16S rRNA genes, and (15)N-tracer incubations. Here we show that anammox performing organisms were abundant bacterial community members. Although all sites were dominated by Candidatus Brocadia-like sequences, the community at one site was particularly diverse, possessing four of five known genera of anammox bacteria. Isotope data showed that anammox produced up to 18 and 36% of N(2) at these sites. By combining molecular and isotopic results we have demonstrated the diversity, abundance, and activity of these autotrophic bacteria. Our results provide strong evidence for their important biogeochemical role in attenuating groundwater ammonium contamination.
authors.affiliation: University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Tara A
authors.initials: TA
authors.lastname: Moore
authors.forename: Yangping
authors.initials: Y
authors.lastname: Xing
authors.forename: Brent
authors.initials: B
authors.lastname: Lazenby
authors.forename: Michael D J
authors.initials: MD
authors.lastname: Lynch
authors.forename: Sherry
authors.initials: S
authors.lastname: Schiff
authors.forename: William D
authors.initials: WD
authors.lastname: Robertson
authors.forename: Robert
authors.initials: R
authors.lastname: Timlin
authors.forename: Sadia
authors.initials: S
authors.lastname: Lanza
authors.forename: M Cathryn
authors.initials: MC
authors.lastname: Ryan
authors.forename: Ramon
authors.initials: R
authors.lastname: Aravena
authors.forename: Danielle
authors.initials: D
authors.lastname: Fortin
authors.forename: Ian D
authors.initials: ID
authors.lastname: Clark
authors.forename: Josh D
authors.initials: JD
authors.lastname: Neufeld
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics* / Q000378:metabolism*; D002170:Canada; D060587:Groundwater / Q000737:chemistry* / Q000382:microbiology*; D009587:Nitrogen Isotopes / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D014871:Water Microbiology
chemical_list: D009587:Nitrogen Isotopes; D000644:Quaternary Ammonium Compounds; D012336:RNA, Ribosomal, 16S