title: Porcine model of congenital heart defect with decreased pulmonary blood flow.
abstract: A porcine model was produced to study the pathophysiology of congenital heart defect (CHD) with decreased pulmonary blood flow. Twenty piglets (1-2 months) were randomly divided as mild to moderate stenosis/T(1) group (n = 7) in which artificial atrial septal defect (ASD) with pulmonary artery banding generated a systolic pressure gradient of 20-30 mmHg; severe stenosis/T group (n = 7) group with a systolic pressure gradient of ≥30-50 mmHg; and controls/C group (n = 6) underwent sham surgery. At 1 month postoperatively, 64-slice computed tomography (CT) was performed. At 2 months, left-chest surgery was performed to measure ASD diameter, arterial blood gas, hemoglobin, and Trans-PABP. Our data show successful establishment of porcine CHD model. ASDs in T(1)/T(2) groups were 8.0 ± 0.5/8.9 ± 1.4 mm, respectively. Trans-PABP showed that the pressure increase in T(2) was higher (P < 0.01) than in T(1) group. Arterial blood PaO(2) and SaO(2) of T(1/)T(2) groups were significantly lower than controls. AoD was significantly lower in T(1) than in C group. Balloon dilation was significantly lower than AoD in T(1)/T(2) groups. Besides, one animal in T(1), two animals in T(2) ,and one animal in C group died. We conclude that ASD with pulmonary artery banding is a successful intervention to establish such a model, and our findings may influence the treatment of patients with similar heart disease.
authors.affiliation: Department of Cardiothoracic Surgery, The 1st Affiliated Hospital of Bengbu Medical College, Bengbu, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xuegang
authors.forename: Shi
authors.initials: S
authors.lastname: Chao
authors.forename: Wang
authors.initials: W
authors.lastname: Kangwu
authors.forename: Liu
authors.initials: L
authors.lastname: Yiyao
authors.forename: Duan
authors.initials: D
authors.lastname: Guixin
authors.forename: Li
authors.initials: L
authors.lastname: Xiaohong
authors.forename: Song
authors.initials: S
authors.lastname: Wei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Junxiang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D006330:Heart Defects, Congenital / Q000097:blood / Q000175:diagnosis / Q000473:pathology / Q000503:physiopathology*; D021621:Imaging, Three-Dimensional; D008297:Male; D011651:Pulmonary Artery / Q000503:physiopathology / Q000601:surgery; D011652:Pulmonary Circulation; D013552:Swine; D036542:Tomography, Spiral Computed