title: Phospholipid scramblase 1 mediates hepatitis C virus entry into host cells.
abstract: Hepatitis C virus (HCV) infects human hepatocytes through several host factors. However, other prerequisite factors for viral entry remain to be identified. Using a yeast two-hybrid screen, we found that human phospholipid scramblase 1 interacts with HCV envelope proteins E1 and E2. These physical interactions were confirmed by co-immunoprecipitation and GST pull-down assays. Knocking down the expression of PLSCR1 inhibited the entry of HCV pseudoparticles. Moreover, PLSCR1 was required for the initial attachment of HCV onto hepatoma cells, where it specifically interacted with entry factor OCLN. We show that PLSCR1 is a novel attachment factor for HCV entry.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Gong
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Xiuying
authors.initials: X
authors.lastname: Liu
authors.forename: Youhui
authors.initials: Y
authors.lastname: Si
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Yuan
authors.forename: Chaozhi
authors.initials: C
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D062445:Claudin-1; D016174:Hepacivirus / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D062793:Occludin; D049294:Phospholipid Transfer Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D034622:RNA Interference; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; C568108:CLDN1 protein, human; D062445:Claudin-1; C087035:E1 protein, Hepatitis C virus; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C493422:PLSCR1 protein, human; D049294:Phospholipid Transfer Proteins; C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; D060225:Tetraspanin 28; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus