title: Derivation of mouse embryonic stem cell lines from blastocysts produced by fertilization and somatic cell nuclear transfer.
abstract: This chapter describes a detailed protocol for deriving mouse embryonic stem (ES) cell lines from blastocysts that are produced either by fertilization or by somatic cell nuclear transfer (NT or cloning). Rather than function as a scientific communication with the experts in this field, this protocol is written with researchers who are new to mouse ES cell line derivation in mind. Therefore, researchers who want to establish such methods in laboratories where mouse ES cell line derivation has never been done before should find this chapter helpful.
authors.affiliation: Department of Epigenetics and Embryo Development, Hematech, Inc., Sioux Falls, SD, USA. zwang@hematech.com
authors.forename: Zhongde
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D002460:Cell Line; D015925:Cryopreservation; D004247:DNA / Q000378:metabolism; D018274:Electroporation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016685:Mitomycin / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D013045:Species Specificity; D013997:Time Factors; D014965:X-Rays
chemical_list: D016685:Mitomycin; D004247:DNA