title: DR haplotype diversity of the cynomolgus macaque as defined by its transcriptome.
abstract: The DR region of particular primate species may display allelic polymorphism and gene copy number variation (region configuration polymorphism). The sum of these distinct types of polymorphism is defined as complexity. To date, however, the DR region of cynomolgus macaques (Macaca fascicularis) has been poorly defined. Transcriptome analysis of a pedigreed colony, comprising animals from Indonesia and Indochina, revealed a total of 15 Mafa-DRA and 57 DRB alleles, specifying 28 different region configurations. The DRA alleles can be divided into two distinct lineages. One lineage is polymorphic, but the majority of the amino acid replacements map to the leader peptide. The second lineage is at best oligomorphic, and segregates with one specific Mafa-DRB allele. The number of Mafa-DRB genes ranges from two to five per haplotype. Due to the presence of pseudogenes, however, each haplotype encodes only one to three bona fide DRB transcripts. Depending on the region configuration in which the Mafa-DRB gene is embedded, identical alleles may display differential transcription levels. Region configurations appear to have been generated by recombination-like events. When genes or gene segments are relocated, it seems plausible that they may be placed in the context of distinct transcription control elements. As such, DRB region-related transcription level differences may add an extra layer of polymorphism to this section of the adaptive immune system.
authors.affiliation: Biomedical Primate Research Centre, Department of Comparative Genetics and Refinement, Lange Kleiweg 161, 2288 GJ Rijswijk, The Netherlands. doxiadis@bprc.nl
authors.forename: Gaby G M
authors.initials: GG
authors.lastname: Doxiadis
authors.forename: Annemiek J M
authors.initials: AJ
authors.lastname: de Vos-Rouweler
authors.forename: Nanine
authors.initials: N
authors.lastname: de Groot
authors.forename: Nel
authors.initials: N
authors.lastname: Otting
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Bontrop
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D010375:Pedigree; D011110:Polymorphism, Genetic; D059467:Transcriptome
chemical_list: D006684:HLA-DR Antigens