title: [Methodological study on magnetic affinity immunoassay for detecting chloramphenicol].
abstract: OBJECTIVE
  To establish a sensitive immunoassay for detection chloramphenicol using magnetic beads as solid phase.
  
  
  METHODS
  This assay employs the competitive inhibition, the FITC conjugated with anti-chloramphenicol monoclonal antibodies and the alkaline phosphatase conjugated with chloramphenicol respectively. Magnetic beads were coupled with sheep anti-FITC antibodies as solid phase. And phenolphthalein monophosphate was used as substrate to set up MEIA for detection chloramphenicol.
  
  
  RESULTS
  MEIA for detection chloramphenicol was Established, the reaction time is 40 minutes, the sensitivity is 0.03 microg/L, the liner range is 0.1-8.1 microg/L, the intra and inter coefficient variation (CV) was 3.9%-5.3% and 4.8%-8.1% respectively and the recovery is 97%-101.5%. Comparing with national standard method of liquid chromatography-mass spectrometry, the Correlation coefficient of test results is 0.9817 (r=0.9817).
  
  
  CONCLUSIONS
  The chloramphenicol MEIA method is sensitive, accurate, and fast, it provides a new method of immunoassay for the monitoring of chloramphenicol residues in food.
authors.affiliation: Biology Science and Technology College, Huazhong Science and Technology University, Wuhan, China. zhb0627@sohu.com
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Zhen-shi
authors.initials: ZS
authors.lastname: Liu
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Xiang-liang
authors.initials: XL
authors.lastname: Yang
mesh_terms: D000818:Animals; D002701:Chloramphenicol / Q000032:analysis*; D005396:Fish Products / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D008280:Magnetics / Q000379:methods*
chemical_list: D002701:Chloramphenicol