title: Myricetin suppresses oxidative stress-induced cell damage via both direct and indirect antioxidant action.
abstract: We evaluated the cytoprotective effect of myricetin on oxidative stress damaged cells by assessment of the scavenging effect of reactive oxygen species (ROS) and the activities of antioxidant enzymes. Myricetin showed the scavenging effect of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals on intracellular ROS. In addition, myricetin restored the activity and protein expression of cellular antioxidant defense enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) reduced by hydrogen peroxide (H(2)O(2)) treatment. H(2)O(2)-induced cellular DNA and lipid damages, and myricetin was found to prevent the DNA damage shown by inhibition of DNA tail and it decreased nuclear phospho-histone H2A.X expression, which are both markers for DNA strand breakage. Membrane lipid peroxidation was also attenuated as shown by inhibition of TBARS formation and of fluorescence intensity of diphenyl-1-pyrenylphosphine (DPPP). These results suggest that myricetin protects cells against H(2)O(2)-induced cell damage via inhibition of ROS generation and activation of antioxidant enzymes.
authors.affiliation: School of Medicine, Jeju National University, Jeju-si 690-756, Republic of Korea.
authors.forename: Zhi Hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Kyoung
authors.initials: K
authors.lastname: Ah Kang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Jo
authors.forename: Ju Sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Sam Sik
authors.initials: SS
authors.lastname: Kang
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Deok Hoon
authors.initials: DH
authors.lastname: Park
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun