title: Maternal exposure to carbon black nanoparticle increases collagen type VIII expression in the kidney of offspring.
abstract: The potential health risks of inhaling nanomaterials are of great concern because of their high specific activity and their unique property of translocation. Earlier studies showed that exposure to nanoparticles through the airway affects both respiratory and extrapulmonary organs. When pregnant mice were exposed to nanoparticles, the respiratory system, the central nervous system and the reproductive system of their offspring were affected. The aim of this study was to assess the effect of maternal exposure to nanoparticles on the offspring, particularly on the kidney. Pregnant ICR mice were exposed to a total of 100 µg of carbon black nanoparticle on the fifth and the ninth days of pregnancy. Samples of blood and kidney tissue were collected from 3-week-old and 12-week-old male offspring mice. Collagen expression was examined by quantitative RT-PCR and immunohistochemistry. Serum levels of creatinine and blood urea nitrogen were examined. Exposure of pregnant ICR mice to carbon black resulted in increased expression of Collagen, type VIII, a1 (Col8a1) in the tubular cells in the kidney of 12-week-old offspring mice but not in 3-week-old ones. The levels of serum creatinine and blood urea nitrogen, indices of renal function, were not different between the groups. These observations were similar to those of tubulointerstitial fibrosis in diabetic nephropathy. These results suggest that maternal exposure to carbon black nanoparticle induces renal abnormalities similar to tubulointerstitial fibrosis in diabetic nephropathy are induced in the kidney of offspring.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Umezawa
authors.forename: Sayaka
authors.initials: S
authors.lastname: Kudo
authors.forename: Shinya
authors.initials: S
authors.lastname: Yanagita
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Shinkai
authors.forename: Rikio
authors.initials: R
authors.lastname: Niki
authors.forename: Taito
authors.initials: T
authors.lastname: Oyabu
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ihara
authors.forename: Masao
authors.initials: M
authors.lastname: Sugamata
mesh_terms: D000393:Air Pollutants / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D024102:Collagen Type VIII / Q000096:biosynthesis*; D005260:Female; D005865:Gestational Age; D007150:Immunohistochemistry; D019570:Inhalation Exposure / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000097:blood / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053260:Soot / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D024102:Collagen Type VIII; D053260:Soot