title: Proteomics analysis of porcine serum proteins by LC-MS/MS after foot-and-mouth disease virus (FMDV) infection.
abstract: To analyze serum proteomics differences between normal and foot and mouth disease virus (FMDV)-infected piglets, an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used. Samples of venous blood were collected before and after FMDV infection and high abundance serum albumin was removed using a commercial kit. After trypsin digestion, serum samples were processed with LC-MS/MS. Proteins were identified by peptide mass fingerprinting. We found that apolipoprotein A-IV precursor, haptoglobin and probable chemoreceptor glutamine deamidase cheD appeared after FMDV infection in the same piglet. This is believed to be the first time that serum proteomics analysis by LC-MS/MS after FMDV infection has been performed, and our results may provide further information about biomarkers for early diagnosis of FMD in piglets.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
authors.forename: Keshan
authors.initials: K
authors.lastname: Zhang
authors.forename: Haixue
authors.initials: H
authors.lastname: Zheng
authors.forename: Youjun
authors.initials: Y
authors.lastname: Shang
authors.forename: Jianhong
authors.initials: J
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Tian
authors.forename: Guodong
authors.initials: G
authors.lastname: Lu
authors.forename: Ye
authors.initials: Y
authors.lastname: Jin
authors.forename: Jijun
authors.initials: J
authors.lastname: He
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002853:Chromatography, Liquid; D005536:Foot-and-Mouth Disease / Q000097:blood* / Q000378:metabolism; D010449:Peptide Mapping; D040901:Proteomics; D013552:Swine; D013553:Swine Diseases / Q000097:blood* / Q000821:virology; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins