title: One- and two-photon live cell imaging using a mutant SNAP-Tag protein and its FRET substrate pairs.
abstract: A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543.
authors.forename: Chong-Jing
authors.initials: CJ
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Xu
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival; D006224:Cricetinae; D003412:Cricetulus; D031541:Fluorescence Resonance Energy Transfer; D017785:Photons; D011506:Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D011506:Proteins