title: Detection of a porcine rotavirus strain with VP4, VP7 and NSP4 genes of different animal origins.
abstract: A new rotavirus strain, sh0902, was detected in diarrheic piglets on a farm in Shanghai, China, and its genotype was characterized as G1P[7]. Analysis of the VP4, VP7 and NSP4 genes demonstrated VP4 homology to bovine and swine rotavirus strains; the nucleotide (nt) and amino acid (aa) identities were 99.7% and 99.5%, respectively. The VP7 gene was highly homologous to that of a giant panda rotavirus strain, with 98.5% similarity at the nt level and 99% similarity at the aa level. The nucleotide sequence of the NSP4 gene displayed high homology to human rotavirus strain R479, with 99.7% identity at the nt level and 99.3% identity at the aa level. This is the first report of an unusual porcine rotavirus strain with VP4, VP7 and NSP4 genes that are highly homologous to bovine, swine, giant panda and human strains isolated at geographically distant sites (South Korea, China and India). Our data indicate that rotaviruses have circulated among humans and animals and undergone genome reassortment.
authors.affiliation: Institute of Animal Husbandry Veterinary Sciences, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai 201106, China. yijianzhong@yahoo.com
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Yi
authors.forename: Chenqian
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D056910:Republic of Korea; D012401:Rotavirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology*; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014118:Toxins, Biological / Q000737:chemistry / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; D014118:Toxins, Biological; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D017361:Viral Nonstructural Proteins