title: Rapid identification of Staphylococcus aureus and methicillin resistance by flow cytometry using a peptide nucleic acid probe.
abstract: A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.
authors.affiliation: Department of Infectious Disease, Cleveland Clinic, 9500 Euclid Avenue/G-21, Cleveland, OH 44195, USA. shrestn@ccf.org
authors.forename: Nabin K
authors.initials: NK
authors.lastname: Shrestha
authors.forename: Nikole M
authors.initials: NM
authors.lastname: Scalera
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Wilson
authors.forename: Byron
authors.initials: B
authors.lastname: Brehm-Stecher
authors.forename: Gary W
authors.initials: GW
authors.lastname: Procop
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016106:Methicillin Resistance; D020135:Peptide Nucleic Acids; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D020135:Peptide Nucleic Acids