title: IL-10 is predominantly produced by CD19(low)CD5(+) regulatory B cell subpopulation: characterisation of CD19 (high) and CD19(low) subpopulations of CD5(+) B cells.
abstract: IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10, and Annexin V. Interestingly, IL-10-producing B cells were found to be localised within the CD19(low)CD5(+) B cell subset. Apoptotic changes were also observed mainly in CD19(low) cells among B cells. Thus, CD5(+) B cells should be classified as CD19(high) and CD19(low) cells, and the immunological significance of CD19 for the IL-10 production by CD5(+) B cells requires further studies.
authors.affiliation: Department of Paediatrics, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Joonyong
authors.initials: J
authors.lastname: Noh
authors.forename: Geunwoong
authors.initials: G
authors.lastname: Noh
authors.forename: Wahn Soo
authors.initials: WS
authors.lastname: Choi
authors.forename: Sang Sun
authors.initials: SS
authors.lastname: Lee
mesh_terms: D018941:Antigens, CD19 / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D018956:CD5 Antigens / Q000378:metabolism; D002469:Cell Separation; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*
chemical_list: D018941:Antigens, CD19; D018956:CD5 Antigens; C508609:IL10 protein, human; D016753:Interleukin-10