title: Analysis of group A Streptococcus gene expression in humans with pharyngitis using a microarray.
abstract: Pharyngitis caused by group A streptococci (GAS) is one of the most common infections around the world. However, relatively little is known about which genes are expressed and which genes regulate expression during acute infection. Due to their ability to provide genome-wide views of gene expression at one time, microarrays are increasingly being incorporated in GAS research. In this study, a novel electrochemical detection-based microarray was used to identify gene expression patterns among humans with culture-confirmed GAS pharyngitis. Using 14 samples (11 GAS-positive and three GAS-negative) obtained from subjects seen at the Brooke Army Medical Center paediatric clinic, this study demonstrated two different clusters of gene expression patterns. One cluster expressed a larger number of genes related to phages, immune-system evasion and survival among competing oral flora, signifying a potentially more virulent pattern of gene expression. The other cluster showed a greater number of genes related to nutrient acquisition and protein expression. This in vivo genome-wide analysis of GAS gene expression in humans with pharyngitis evaluated global gene expression in terms of virulence factors.
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Livezey
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Luis
authors.initials: L
authors.lastname: Perez
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Dominic
authors.initials: D
authors.lastname: Suciu
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Robinson
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: David
authors.initials: D
authors.lastname: Bush
authors.affiliation: Department of Clinical Investigations, Brooke Army Medical Center, 3400 Rawley E. Chambers Ave, Suite A, San Antonio, TX 78234, USA.
authors.forename: Gerald
authors.initials: G
authors.lastname: Merrill
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001422:Bacterial Adhesion / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D017136:Ion Transport / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods; D010612:Pharyngitis / Q000382:microbiology*; D013290:Streptococcal Infections / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D013312:Stress, Physiological / Q000235:genetics; D059467:Transcriptome; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D037521:Virulence Factors