title: Lysine acetylation sites in bovine foamy virus transactivator BTas are important for its DNA binding activity.
abstract: Cellular acetylation signaling is important for viral gene regulation, particularly during the transactivation of retroviruses. The regulatory protein of bovine foamy virus (BFV), BTas, is a transactivator that augments viral gene transcription from both the long terminal repeat (LTR) promoter and the internal promoter (IP). In this study, we report that the histone acetyltransferase (HAT), p300, specifically acetylates BTas both in vivo and in vitro. Further studies demonstrated that BTas acetylation markedly enhances its transactivation activity. Mutagenesis analysis identified three lysines at positions 66, 109 and 110 in BTas that are acetylated by p300. The K110R mutant lost its binding to BFV promoter as well as its ability to activate BFV promoter. The acetylation of K66 and K109 may contribute to increased BTas binding ability. These results suggest that the p300-acetylated lysines of BTas are important for transactivation of BFV promoters and therefore have an important role in BFV replication.
authors.affiliation: Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education), College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chang
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Fengwen
authors.initials: F
authors.lastname: Xu
authors.forename: Hongqi
authors.initials: H
authors.lastname: Han
authors.forename: Yunqi
authors.initials: Y
authors.lastname: Geng
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.forename: Wentao
authors.initials: W
authors.lastname: Qiao
mesh_terms: D000107:Acetylation; D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004252:DNA Mutational Analysis; D054884:Host-Pathogen Interactions; D006801:Humans; D008239:Lysine / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D016092:Spumavirus / Q000235:genetics / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D050880:p300-CBP Transcription Factors / Q000378:metabolism*; D000099224:p300-CBP-Associated Factor
chemical_list: D004247:DNA; D008239:Lysine; D015534:Trans-Activators; D014764:Viral Proteins; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor