title: Nanoparticles prepared from the water extract of Gusuibu (Drynaria fortunei J. Sm.) protects osteoblasts against insults and promotes cell maturation.
abstract: Our previous study showed that Gusuibu (Drynaria fortunei J. Sm.) can stimulate osteoblast maturation. This study was further designed to evaluate the effects of nanoparticles prepared from the water extract of Gusuibu (WEG) on osteoblast survival and maturation. Primary osteoblasts were exposed to 1, 10, 100, and 1000 μg/mL nanoparticles of WEG (nWEG) for 24, 48, and 72 hours did not affect morphologies, viability, or apoptosis of osteoblasts. In comparison, treatment of osteoblasts with 1000 μg/mL WEG for 72 hours decreased cell viability and induced DNA fragmentation and cell apoptosis. nWEG had better antioxidant bioactivity in protecting osteoblasts from oxidative and nitrosative stress-induced apoptosis than WEG. In addition, nWEG stimulated greater osteoblast maturation than did WEG. Therefore, this study shows that WEG nanoparticles are safer to primary osteoblasts than are normal-sized products, and may promote better bone healing by protecting osteoblasts from apoptotic insults, and by promoting osteogenic maturation.
authors.affiliation: Institute of Materials Science and Engineering, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Chung-King
authors.initials: CK
authors.lastname: Hsu
authors.forename: Mei-Hsiu
authors.initials: MH
authors.lastname: Liao
authors.forename: Yu-Tyng
authors.initials: YT
authors.lastname: Tai
authors.forename: Shing-Hwa
authors.initials: SH
authors.lastname: Liu
authors.forename: Keng-Liang
authors.initials: KL
authors.lastname: Ou
authors.forename: Hsu-Wei
authors.initials: HW
authors.lastname: Fang
authors.forename: I-Jung
authors.initials: IJ
authors.lastname: Lee
authors.forename: Ruei-Ming
authors.initials: RM
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D009599:Nitroprusside / Q000008:administration & dosage / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D029621:Polypodiaceae / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D014867:Water / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014867:Water; D009599:Nitroprusside; D006861:Hydrogen Peroxide