title: Inflammatory response of porcine epithelial IPEC J2 cells to enterotoxigenic E. coli infection is modulated by zinc supplementation.
abstract: Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in pigs and humans. The duration and severity of diarrhoea can be controlled using zinc supplementation, typically pharmacological levels of zinc oxide in pigs. In this study, IPEC J2 cells were used as an in vitro model of intestinal ETEC infection, with separate and simultaneous zinc treatment. Genomic analysis identified increased expression of a variety of innate immune response genes (NF-κB targets) in response to ETEC exposure, and several stress response genes in response to zinc exposure, provided as ZnO. Expression of genes involved in the innate immune response was reduced when cells were simultaneously exposed to ZnO, and it is suggested that ZnO treatment inhibits the induction of NF-κB in response to pathogens, possibly through up-regulated heat shock proteins. A similar response in vivo with consequent down-regulation in the inflammatory response would reduce further pathogen invasion, maintain normal gut function and maintain growth.
authors.affiliation: Faculty of Biological Sciences, University of Leeds, Clarendon Road, Leeds LS2 9JT, United Kingdom.
authors.forename: Hannah R
authors.initials: HR
authors.lastname: Sargeant
authors.forename: Helen M
authors.initials: HM
authors.lastname: Miller
authors.forename: Marie-Anne
authors.initials: MA
authors.lastname: Shaw
mesh_terms: D000818:Animals; D002460:Cell Line; D054307:Enterotoxigenic Escherichia coli; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology; D004927:Escherichia coli Infections / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D007155:Immunologic Factors / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D016328:NF-kappa B; D015034:Zinc Oxide