title: Ex vivo demonstration of a synergistic effect of Adapalene and benzoyl peroxide on inflammatory acne lesions.
abstract: Acne is a chronic inflammatory disease of the pilosebaceous follicle. Thanks to its ability to reduce both comedones and inflammatory lesions, the association of a retinoid and benzoyl peroxide (BPO) is now recommended for the treatment of acne. However, the mechanisms of action of this combined therapy on inflammatory acne lesions are not well understood. In an ex vivo immunohistochemistry study, we investigated the potential synergistic modulator effect of Adapalene associated with BPO on keratinocytes proliferation/differentiation and innate immunity in inflammatory acne lesions. We demonstrated that proliferation (Ki-67), adhesion/differentiation (integrin α(2), α(3) and α(6)) and innate immunity (TLR-2, β-defensin 4, IL-8) markers are overexpressed in inflammatory acne skin compared with uninvolved acne skin. Association of Adapalene and BPO significantly decreased expression of Ki67, α(2) and α(6) integrins, TLR-2, β-defensin 4 and IL-8 in inflammatory acne skin, whereas single treatments with Adapalene or BPO alone were less effective. These results contribute to explain the comedolytic and anti-inflammatory activities of this combined therapy observed in recent clinical trials.
authors.forename: Thomas
authors.initials: T
authors.lastname: Zuliani
authors.forename: Amir
authors.initials: A
authors.lastname: Khammari
authors.forename: Hélène
authors.initials: H
authors.lastname: Chaussy
authors.forename: Anne Chantal
authors.initials: AC
authors.lastname: Knol
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dréno
mesh_terms: D000152:Acne Vulgaris / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000068816:Adapalene; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D001585:Benzoyl Peroxide / Q000008:administration & dosage*; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003879:Dermatologic Agents / Q000008:administration & dosage; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D016023:Integrins / Q000378:metabolism; D008297:Male; D009281:Naphthalenes / Q000008:administration & dosage*; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003879:Dermatologic Agents; D016023:Integrins; D009281:Naphthalenes; D023083:beta-Defensins; D000068816:Adapalene; D001585:Benzoyl Peroxide