title: Determination Co2+ in vitamin B12 based on enhancement of 2-(4-substituted-phenyl)-4,5-di(2-furyl) imidazole and H2O2 chemiluminescence reaction.
abstract: In this paper, three kinds of imidazole derivatives, 2-(4-methylphenyl)-4,5-di(2-furyl) imidazole (MDFI), 2-(4-nitrophenyl)-4,5-di(2-furyl) imidazole (NDFI), and 2-(4-tert-butylphenyl)-4,5-di(2-furyl) imidazole (t-BDFI) were synthesized. In an alkaline medium, the chemiluminescence (CL) reaction of imidazole derivatives with H(2)O(2) has been investigated. It was also found that MDFI/H(2)O(2) and t-BDFI/H(2)O(2) systems gave strong CL. When Co(2+) was added into the two CL systems, the CL intensity was remarkably enhanced. In the optimum conditions, the CL intensity is linearly related to the logarithm of concentration of Co(2+). The linear ranges are 5×10(-9)-2.5×10(-7) mol/L for MDFI/H(2)O(2) system and 5×10(-9)-2.5×10(-7) mol/L for t-BDFI/H(2)O(2) system, and the corresponding detection limits are 1.2×10(-9) mol/L and 1.1×10(-9) mol/L, respectively. The method was applied to the determination of Co(2+) in vitamin B(12) injection. Furthermore, the CL mechanism was also discussed.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Han
authors.forename: Yumin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Kang
authors.forename: Jieli
authors.initials: J
authors.lastname: Tang
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002621:Chemistry / Q000379:methods*; D003035:Cobalt / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004867:Equipment Design; D006861:Hydrogen Peroxide / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D007477:Ions; D049449:Luminescence; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008956:Models, Chemical; D009596:Nitrophenols / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods*; D014805:Vitamin B 12 / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D007477:Ions; D009596:Nitrophenols; C024719:4-nitrophenyl; D003035:Cobalt; C029899:imidazole; D006861:Hydrogen Peroxide; D014805:Vitamin B 12