title: Acute differential effects of milk-derived dietary proteins on postprandial lipaemia in obese non-diabetic subjects.
abstract: OBJECTIVE
  Postprandial lipaemia is an established risk factor for atherosclerosis. To investigate the acute effect of four milk-derived dietary proteins (alpha-lactalbumin, whey isolate, caseinoglycomacropeptide and whey hydrolysate) on postprandial lipaemia, we have conducted a randomized, acute, single-blinded clinical intervention study with crossover design.
  
  
  METHODS
  A total of 11 obese non-diabetic subjects (age: 44-74, BMI: 30-41.4 kg m(-2)) were included. On 4 different days the subjects ingested a high-fat meal with the following energy distribution: 66% energy from fat (100 g of butter), 15% of energy from carbohydrate (90 g of white wheat bread) and 19% of energy from protein (45 g of pure protein). Our primary variable was plasma triglyceride measured in the 8-h postprandial period. Secondarily, retinyl palmitate, non-esterified free fatty acids, glucose, insulin, glucagon, GLP-1 and GIP, active and total grehlin and cholecystokinin were measured.
  
  
  RESULTS
  We observed no statistically significant (P=0.8) differences between meals on our primary variable that is, triglycerides. Whey hydrolysate was associated with a significantly (P=0.02) smaller postprandial suppression of non-esterified free fatty acids compared with the other dietary proteins.
  
  
  CONCLUSIONS
  We did not observe significant differences in postprandial lipaemia to the four milk-derived dietary proteins. Whey hydrolysate caused less postprandial suppression of free fatty acids.
authors.affiliation: Department of Endocrinology and Metabolism MEA, Aarhus University Hospital, Aarhus, Denmark. holmer-jensen@ki.au.dk
authors.forename: J
authors.initials: J
authors.lastname: Holmer-Jensen
authors.forename: M L
authors.initials: ML
authors.lastname: Hartvigsen
authors.forename: L S
authors.initials: LS
authors.lastname: Mortensen
authors.forename: A
authors.initials: A
authors.lastname: Astrup
authors.forename: M
authors.initials: M
authors.lastname: de Vrese
authors.forename: J J
authors.initials: JJ
authors.lastname: Holst
authors.forename: C
authors.initials: C
authors.lastname: Thomsen
authors.forename: K
authors.initials: K
authors.lastname: Hermansen
mesh_terms: D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000209:etiology; D003920:Diabetes Mellitus; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000009:adverse effects*; D005230:Fatty Acids, Nonesterified / Q000097:blood*; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000178:diet therapy / Q000209:etiology; D008297:Male; D008875:Middle Aged; D008894:Milk Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D009765:Obesity / Q000097:blood* / Q000178:diet therapy; D019518:Postprandial Period; D012307:Risk Factors; D016037:Single-Blind Method; D014280:Triglycerides / Q000097:blood*
chemical_list: D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D008894:Milk Proteins; D014280:Triglycerides