title: Improved method for analyzing the degradation of estrogens in water by solid-phase extraction coupled with ultra performance liquid chromatography-ultraviolet detection.
abstract: We established an improved method for the determination of four estrogens including estriol (E3), 17beta-estradiol (E2), 17alpha-ethynylestrodiol (EE2) and estrone (El) in water. The method consisted of solid-phase extraction (0.5 L water) and subsequent analysis of analytes by ultra-performance liquid chromatography (UPLC) with an ultraviolet detector (UVD). Base-line separation was achieved for all studied estrogens using a column (50 mm x 2.1 mm) packed with 1.7 microm particle size stationary phase. Recovery was higher than 88% and detection limits ranged between 12.5-23.7 ng/L for the four estrogens, with the RSD ranging from 7% to 11%. The method was successfully applied to determine E2 and EE2 in simulated natural water, which found that about 70% of E2 was degraded (with a half-life of about 30 hr) within 48 hr and about 55% of EE2 was degraded (with a half-life of about 36 hr). Low levels of E1 were found, however E3 was undetectable during the process.
authors.affiliation: The First Institute of Oceanography, Qingdao 266101, China. zmg@fio.org.cn
authors.forename: Minggang
authors.initials: M
authors.lastname: Zheng
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Yuandui
authors.initials: Y
authors.lastname: Bi
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D004967:Estrogens / Q000032:analysis* / Q000737:chemistry*; D005399:Fishes; D007700:Kinetics; D057230:Limit of Detection; D052616:Solid Phase Extraction / Q000379:methods*; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry*
chemical_list: D004967:Estrogens; D014867:Water