title: Stella-Cre mice are highly efficient Cre deleters.
abstract: Cre-loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella-Cre, where Cre expression cassette was targeted to the 3' UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre-loxP recombination efficiency in Stella-Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre-loxP recombination efficiency in the embryos and in the germline. Thus, Stella-Cre mice serve as a very efficient deleter line.
authors.affiliation: College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Su-Kit
authors.initials: SK
authors.lastname: Chew
authors.forename: Song-Choon
authors.initials: SC
authors.lastname: Lee
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: George S
authors.initials: GS
authors.lastname: Vassiliou
authors.forename: Tony
authors.initials: T
authors.lastname: Green
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Futreal
authors.forename: Allan
authors.initials: A
authors.lastname: Bradley
authors.forename: Shujun
authors.initials: S
authors.lastname: Zhang
authors.forename: Pentao
authors.initials: P
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001287:Attachment Sites, Microbiological / Q000235:genetics; D017101:Bacteriophage P1 / Q000235:genetics; D001755:Blastocyst; D002868:Chromosomal Proteins, Non-Histone; D004622:Embryo, Mammalian / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D005854:Germ Cells / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D053614:Janus Kinase 2 / Q000235:genetics; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000235:genetics; D000090243:Nucleophosmin; D011506:Proteins / Q000235:genetics; D022661:RNA, Untranslated; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D002868:Chromosomal Proteins, Non-Histone; C462180:Dppa3 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C000715570:Npm1 protein, mouse; D009687:Nuclear Proteins; D011506:Proteins; D022661:RNA, Untranslated; D012097:Repressor Proteins; D000090243:Nucleophosmin; C507926:Jak2 protein, mouse; D053614:Janus Kinase 2; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase