title: The additional N-glycosylation site of the equine LH/CG receptor is not responsible for the limited cyclic AMP pathway activation by equine chorionic gonadotropin relative to luteinizing hormone.
abstract: In order to investigate the role of the unique seventh N23-glycosylation site of the equine LH/CG receptor (eLHCGR) in the cAMP pathway activation, COS-7 cells were transiently transfected with either the wild-type or the mutant eLHCGR(N23Q) cDNA and challenged with porcine LH and eCG for cAMP production. We showed that the N23-glycosylation site of the eLHCGR is not required for the functional coupling of the receptor with the cAMP pathway and is not responsible for the limited potency of eCG relative to pLH to activate this receptor.
authors.affiliation: AgroParisTech, UFR Génétique Elevage Reproduction, 16 rue Claude Bernard, F-75231 Paris, France. stdizier@agroparistech.fr
authors.forename: Marie
authors.initials: M
authors.lastname: Saint-Dizier
authors.forename: Florence
authors.initials: F
authors.lastname: Foulon-Gauze
authors.forename: François
authors.initials: F
authors.lastname: Lecompte
authors.forename: Yves
authors.initials: Y
authors.lastname: Combarnous
authors.forename: Maryse
authors.initials: M
authors.lastname: Chopineau
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006031:Glycosylation; D006736:Horses; D007986:Luteinizing Hormone / Q000494:pharmacology*; D011485:Protein Binding; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*
chemical_list: D006063:Chorionic Gonadotropin; D011974:Receptors, LH; D007986:Luteinizing Hormone; D000242:Cyclic AMP