title: Ternatin pretreatment attenuates testicular injury induced by torsion/detorsion in Wistar rats.
abstract: OBJECTIVE
  To investigate the possible protective role of the bioflavonoid ternatin (TTN) when administered before induction of ischemia/reperfusion injury in rat testis.
  
  
  METHODS
  Thirty-six Wistar rats were randomly assigned to 3 groups (n=12), divided in 2 subgroups (n=6). Saline 2.0ml (G-1), dimethylsulfoxide (DMSO) 3% solution (G-2) or TTN 12 mg/kg/dose (G-3) was administered ip. to all rats, respectively, 21, 12 and 1 hour before torsion. Anesthetized rats were subjected to ischemia (3 hours) induced by 720º torsion of the spermatic cord. Right testis and arterial blood samples were collected at the end of ischemia (T-0), and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP).
  
  
  RESULTS
  MDA decreased significantly (p<0,001) in G-2 and G-3 in T-0 and T-3 timepoints. Additional decrease in MDA was seen in G-3 after 3 hours of reperfusion (T-3). GSH increased significantly in G-2 (p<0.001) and G-3 (p<0.05) at the end the ischemia (T-0). A significant increase in GSH was seen 3 hours after testis detorsion (T-3) in G-2 rats. TAP values remained unchanged.
  
  
  CONCLUSIONS
  The data provides in vivo evidence of the antiperoxidative and antioxidative properties of TTN in torted rat testis.
authors.affiliation: LABCEX, Department of Surgery, UFC, Ceara, Brazil. sergiobotelho@terra.com.br
authors.forename: Sergio Botelho
authors.initials: SB
authors.lastname: Guimarães
authors.forename: Jefferson Menezes Viana
authors.initials: JM
authors.lastname: Santos
authors.forename: Alan Arruda
authors.initials: AA
authors.lastname: Aragão
authors.forename: Osamu Sandes
authors.initials: OS
authors.lastname: Kimura
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.forename: Paulo Roberto Leitão de
authors.initials: PR
authors.lastname: Vasconcelos
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D005419:Flavonoids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D007511:Ischemia / Q000517:prevention & control*; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013086:Spermatic Cord Torsion / Q000517:prevention & control*; D013737:Testis / Q000098:blood supply*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005419:Flavonoids; C051080:ternatin (flavonoid); D005978:Glutathione