title: Development of a simple PCR-based assay for the identification of triazine resistance in the noxious plant common ragweed (Ambrosia artemisiifolia) and its applicability in higher plants.
abstract: Bidirectional allele-specific PCR (Bi-PASA) was used to detect a point mutation causing triazine resistance in common ragweed (Ambrosia artemisiifolia). The external primers amplified a 278 bp standard DNA fragment in all genotypes. In the susceptible S264S genotypes, a 208 bp fragment was expected while in resistant S264G common ragweed genotypes a 109 bp band was expected. In resistant plants, both the wild and mutant type fragments were detected, indicating that the original triazine sensitive cpDNA is maintained in a heteroplasmic state in the resistant S264G genotypes. Additionally, in silico analysis confirmed the potential applicability of our diagnostic assay for other plant species. In 24 out of 74 taxa (32%), the assay could be used without any change, while in the others some of the primers should be redesigned before use.
authors.affiliation: Biotechnology Group, Department of Plant Science and Biotechnology, Georgikon Faculty, University of Pannonia, Festetics u. 7, Keszthely 8360, Hungary.
authors.forename: Kinga Klára
authors.initials: KK
authors.lastname: Mátyás
authors.forename: János
authors.initials: J
authors.lastname: Taller
authors.forename: András
authors.initials: A
authors.lastname: Cseh
authors.forename: Péter
authors.initials: P
authors.lastname: Poczai
authors.forename: István
authors.initials: I
authors.lastname: Cernák
mesh_terms: D031164:Ambrosia / Q000187:drug effects* / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D014226:Triazenes / Q000494:pharmacology*
chemical_list: D018744:DNA, Plant; D014226:Triazenes