title: Sulfur amino acid deprivation in cadmium chloride toxicity in hepatoma cells.
abstract: The aim of this study was to investigate the effect of individual sulfur amino acid deprivation in cadmium chloride toxicity. HTC cells were deprived of cystine and/or methionine for 12h and then exposed to CdCl(2) for 12h. HepG2 cells were deprived of cystine for 3 and 5h and exposed to CdCl(2) for 3h. In addition HepG2 cells were deprived of methionine for 12h and then exposed to CdCl(2) for 5 and 12h. Our results indicate that only cystine depletion increased cadmium toxicity in HTC cells but not in HepG2 cells as indicated by the neutral red assay. This effect was due to glutathione depletion as indicated by measurement of intracellular glutathione in HTC cells following deprivation of cystine. Methionine depletion had only a slight effect on the viability of HepG2 cells.
authors.affiliation: 150 Stamford street, Franklin Wilkins Building, Pharmacy Department, King's College London, London SE1 8WA, UK.
authors.forename: George
authors.initials: G
authors.lastname: Fotakis
authors.forename: John A
authors.initials: JA
authors.lastname: Timbrell