title: Use of Mutant-Assisted Gene Identification and Characterization (MAGIC) to identify novel genetic loci that modify the maize hypersensitive response.
abstract: The partially dominant, autoactive maize disease resistance gene Rp1-D21 causes hypersensitive response (HR) lesions to form spontaneously on leaves and stems in the absence of pathogen recognition. The maize nested association mapping (NAM) population consists of 25 200-line subpopulations each derived from a cross between the maize line B73 and one of 25 diverse inbred lines. By crossing a line carrying the Rp1-D21 gene with lines from three of these subpopulations and assessing the F(1) progeny, we were able to map several novel loci that modify the maize HR, using both single-population quantitative trait locus (QTL) and joint analysis of all three populations. Joint analysis detected QTL in greater number and with greater confidence and precision than did single population analysis. In particular, QTL were detected in bins 1.02, 4.04, 9.03, and 10.03. We have previously termed this technique, in which a mutant phenotype is used as a "reporter" for a trait of interest, Mutant-Assisted Gene Identification and Characterization (MAGIC).
authors.affiliation: Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Vijay
authors.initials: V
authors.lastname: Chaikam
authors.forename: Adisu
authors.initials: A
authors.lastname: Negeri
authors.forename: Rahul
authors.initials: R
authors.lastname: Dhawan
authors.forename: Bala
authors.initials: B
authors.lastname: Puchaka
authors.forename: Jiabing
authors.initials: J
authors.lastname: Ji
authors.forename: Satya
authors.initials: S
authors.lastname: Chintamanani
authors.forename: Emma W
authors.initials: EW
authors.lastname: Gachomo
authors.forename: Allen
authors.initials: A
authors.lastname: Zillmer
authors.forename: Timothy
authors.initials: T
authors.lastname: Doran
authors.forename: Cliff
authors.initials: C
authors.lastname: Weil
authors.forename: Peter
authors.initials: P
authors.lastname: Balint-Kurti
authors.forename: Guri
authors.initials: G
authors.lastname: Johal
mesh_terms: D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D014644:Genetic Variation; D047908:Intracellular Signaling Peptides and Proteins; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci; D003313:Zea mays / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010940:Plant Proteins; C121068:Rp1-D protein, Zea mays