title: Characterization of methane oxidation by a methanotroph isolated from a landfill cover soil, South Korea.
abstract: A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol·g--dry cell weight--1·h⁻¹ and 23 microM, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and p-xylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds.
authors.affiliation: Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Taewoo
authors.initials: T
authors.lastname: Yi
authors.forename: Kyung-Eun
authors.initials: KE
authors.lastname: Moon
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Park
authors.forename: Hee Wook
authors.initials: HW
authors.lastname: Ryu
authors.forename: Kyung-Suk
authors.initials: KS
authors.lastname: Cho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006844:Hydrocarbons, Cyclic / Q000494:pharmacology; D007700:Kinetics; D008697:Methane / Q000378:metabolism*; D042083:Methylocystaceae / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013457:Sulfur Compounds / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006844:Hydrocarbons, Cyclic; D012336:RNA, Ribosomal, 16S; D013457:Sulfur Compounds; D008697:Methane