title: Total reactive antioxidant potential and DNA fragmentation index as fertility sperm parameters.
abstract: There is a growing evidence that oxidative stress play a major role in the etiology of defective sperm function including impaired morphology, motility, metabolism and fertility. The aim of the present study was to examine: 1/ total reactive antioxidant potential (TRAP) in seminal plasma; 2/ sperm DNA fragmentation index (DFI), 3/ sperm morphology and motility and 4/ cellular membrane integrity (hypoosmotic swelling test: HOS test) in patients attending in vitro fertilization/intracytoplasmatic sperm injection ( IVF/ICSI) program. According to the DFI value, the men were divided into: group 1 with DFI ≤15% (n=38) and group 2 with DFI ≥15% (n=37). Significant differences between the two groups were found in TRAP, sperm motility, morphology and concentration as well as HOS test scores. In group 1, DFI was negatively correlated with sperm motility and HOS test scores (p<0.05). The sperm morphology was positively correlated with sperm motility and HOS test scores in both groups. There was no correlation between TRAP and sperm chromatin fragmentation. Our results suggest that seminal plasma TRAP level may be a DFI independent parameter of sperm fertility.
authors.affiliation: Novomedica, Infertility Clinic, 41-400 Myslowice, Bonczyka Street 41, Poland. novomed@ka.onet.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Miciński
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Pawlicki
authors.forename: Ewa
authors.initials: E
authors.lastname: Wielgus
authors.forename: Michał
authors.initials: M
authors.lastname: Bochenek
authors.forename: Piotr
authors.initials: P
authors.lastname: Gogol
authors.forename: Beata
authors.initials: B
authors.lastname: Ficek
mesh_terms: D000328:Adult; D000975:Antioxidants; D002843:Chromatin; D053938:DNA Fragmentation; D006801:Humans; D008297:Male; D017382:Reactive Oxygen Species; D012661:Semen / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D002843:Chromatin; D017382:Reactive Oxygen Species