title: Identification of a sugar beet BvM14-MADS box gene through differential gene expression analysis of monosomic addition line M14.
abstract: Monosomic addition line M14 carrying an additional chromosome 9 from Beta corolliflora Zosimovic ex Buttler was obtained through hybridization between the wild species B. corolliflora and a cultivated species Beta vulgaris L. var Saccharifera Alef. The M14 line showed diplosporic reproduction and stress tolerance. To identify differentially expressed genes in M14, a subtractive cDNA library was prepared by suppression subtractive hybridization (SSH) between M14 (2n=18+1) and B. vulgaris (2n=18). A total of 190 unique sequences were identified in the library and their putative functions were analyzed using Gene Ontology (GO). One of the genes, designated as BvM14-MADS box, encodes a MADS box transcription factor. It was cloned from M14 and over-expressed in transgenic tobacco plants. Interestingly, this gene was located on chromosome 2 of B. vulgaris, not on the additional chromosome 9. Overexpression of BvM14-MADS box led to significant phenotypic changes in tobacco. The differential expression of BvM14-MADS box gene in M14 may be caused by the interaction between the additional chromosome 9 from B. corolliflora and the B. vulgaris chromosomes in M14.
authors.affiliation: College of Life Sciences, Heilongjiang University, Harbin 150080, China.
authors.forename: Chunquan
authors.initials: C
authors.lastname: Ma
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuting
authors.initials: Y
authors.lastname: Wang
authors.forename: Lifa
authors.initials: L
authors.lastname: Wang
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D027461:Beta vulgaris / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003001:Cloning, Molecular; D035264:Flowers / Q000033:anatomy & histology; D015870:Gene Expression / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D015723:Gene Library; D018519:Meristem / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009006:Monosomy / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors