title: Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies.
abstract: Here, we report the construction of a vaccine against lymphocystis disease virus (LCDV) using nucleic acid vaccination technology. A fragment of the major capsid protein encoding gene from an LCDV isolated from China (LCDV-cn) was cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV-cn0.6 kb. This plasmid was immediately expressed after liposomal transfer into the Japanese flounder embryo cell line. The recombinant plasmid was inoculated into Japanese flounder via two routes (intramuscular injection and hypodermic injection) at three doses (0.1, 5, and 15 μg), and then T-lymphopoiesis in different tissues and antibodies raised against LCDV were evaluated. The results indicated that this recombinant plasmid induced unique humoral or cell-mediated immune responses depending on the inoculation route and conferred immune protection. Furthermore, the humoral immune responses and protective effects were significantly increased at higher vaccine doses via the two injection routes. Plasmid pEGFP-N2-LCDV0.6 kb is therefore a promising vaccine candidate against LCDV in Japanese flounder.
authors.affiliation: First Institute of Oceanography, State Oceanography Administration of China, 6 Xianxialing Road, Qingdao City, Shandong Province 266061, China.
authors.forename: Fengrong
authors.initials: F
authors.lastname: Zheng
authors.forename: Xiuqin
authors.initials: X
authors.lastname: Sun
authors.forename: Xing'an
authors.initials: X
authors.lastname: Wu
authors.forename: Hongzhan
authors.initials: H
authors.lastname: Liu
authors.forename: Jiye
authors.initials: J
authors.lastname: Li
authors.forename: Suqi
authors.initials: S
authors.lastname: Wu
authors.forename: Jinxing
authors.initials: J
authors.lastname: Zhang