title: Identification of heat-shock protein 90 beta in Japanese encephalitis virus-induced secretion proteins.
abstract: Five host cellular proteins were identified in the secretion medium from Japanese encephalitis virus (JEV)-infected baby hamster kidney-21 (BHK-21) cells, including three molecular chaperones: Hsp70, GRP78 and Hsp90. Hsp90 isoforms were characterized further. Hsp90α was observed to be retained inside the nuclei, whereas Hsp90β associated with virus particles during assembly and was released into the secretion medium upon JEV infection. The association of Hsp90β and viral E protein was demonstrated by using sucrose-density fractionation and Western blot analysis. Moreover, JEV viral RNA replication was not affected by treatment with geldanamycin, an Hsp90 inhibitor, but impaired virus infectivity that was determined by a plaque-forming assay. Our results show that Hsp90β, not Hsp90α, is present in the JEV-induced secretion medium and is required for JEV infectivity in BHK-21 cells.
authors.affiliation: Department of Biomedical Sciences, Chang Gung University, Tao-Yuan 33302, Taiwan, ROC.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Hung
authors.affiliation: Department of Biomedical Sciences, Chang Gung University, Tao-Yuan 33302, Taiwan, ROC.
authors.forename: Meng-Chieh
authors.initials: MC
authors.lastname: Tsai
authors.affiliation: Department of Biomedical Sciences, Chang Gung University, Tao-Yuan 33302, Taiwan, ROC.
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Wu
authors.affiliation: Research Center for Emerging Viral Infections, Chang Gung University, Tao-Yuan 33302, Taiwan, ROC.|Department of Biomedical Sciences, Chang Gung University, Tao-Yuan 33302, Taiwan, ROC.
authors.forename: Robert Y L
authors.initials: RYL
authors.lastname: Wang
mesh_terms: D000818:Animals; D016227:Benzoquinones / Q000494:pharmacology; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D020033:Protein Isoforms / Q000302:isolation & purification / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D016227:Benzoquinones; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D020033:Protein Isoforms; D012367:RNA, Viral; D014764:Viral Proteins; C001277:geldanamycin