title: [Effects of the IbpAB AND ClpA chaperones on DnaKJE-dependent refolding of bacterial luciferases in Escherichia coli cells].
abstract: The rate and level of DnaKJE-dependent refolding of the thermoinactivated Aliivibrio fischeri luciferase are considerably lower in Escherichia coli ibpA and ibpB mutants than in wild type cells. The rate and level of refolding are lower in E. coli ibpB::kan than in ibpA::kan cells. The decline of refoldings level in E. coli clpA::kan makes progress only with the increase of thermoinactivation time of luciferase. Plasmids with the genes ibpAB don't compensate clpA mutation. It is supposed that small chaperones IbpAB and chaperone ClpA operate independently in a process of DnaKJE-dependent refolding of proteins at the different stages.
authors.forename: O E
authors.initials: OE
authors.lastname: Mel'kina
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Kotova
authors.forename: I V
authors.initials: IV
authors.lastname: Manukhov
authors.forename: G B
authors.initials: GB
authors.lastname: Zavil'gel'skiĭ
mesh_terms: D048908:Aliivibrio fischeri / Q000201:enzymology*; D004258:DNA Polymerase III / Q000378:metabolism; D049071:Endopeptidase Clp / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D050956:HSP40 Heat-Shock Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D049453:Luciferases, Bacterial / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D058849:Protein Refolding
chemical_list: C494635:DnaJ protein, E coli; D029968:Escherichia coli Proteins; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C097578:IbpA protein, E coli; C097579:IbpB protein, E coli; D049453:Luciferases, Bacterial; C074904:DNA polymerase III, alpha subunit; D004258:DNA Polymerase III; C483568:ClpA protease, E coli; D049071:Endopeptidase Clp; C063842:dnaK protein, E coli