title: Elevated expression of KiSS-1 in placenta of preeclampsia and its effect on trophoblast.
abstract: The expression of KiSS-1, MMP-9 and MMP-2 mRNAs and proteins was studied in placentas of women with preeclampsia (PE, n=47) and women of normal pregnancy (NP; n=30). In addition, KiSS-1 mRNA expression as well as cell growth, proliferation and invasion were examined in JAR cells (human trophoblast cell line) transfected with pcDNA3-KiSS-1vector. Expression of KiSS-1 mRNA and protein was higher (p<0.05) in women with PE compared with that of NP women. In contrast, expression of MMP-9 and MMP-2 was lower (p<0.05) in PE than in NP women. KiSS-1 mRNA was detected in JAR cells successfully transfected with pcDNA3-KiSS-1 gene (JAR-K1, JAR-K2, JAR-K3). KiSS-1 mRNA was not detected in JAR cells transfected with pcDNA3 gene (JAR-P1, JAR-P2) and non-transfected JAR cells. No difference (p>0.05) was observed in cell growth among these three cell types. Invasion ability was significantly lower (p<0.01) in JAR-K1, JAR-K2 and JAR-K3 cells compared to JAR-P cells and non-transfected JAR cells. Overexpression of KiSS-1 and insufficient expression of MMP-9 and MMP-2 in placenta were demonstrated in women with PE. The data suggests that KiSS-1 gene plays an important role in inhibiting trophoblast invasion during placental development.
authors.affiliation: Department of Obstetrics and Gynecology of the Second Affiliated Hospital of Soochow University 1055 San Xiang Road, Suzhou, 215004 China. szzhanghong@suda.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Qifang
authors.initials: Q
authors.lastname: Long
authors.forename: Li
authors.initials: L
authors.lastname: Ling
authors.forename: Aihua
authors.initials: A
authors.lastname: Gao
authors.forename: Huifen
authors.initials: H
authors.lastname: Li
authors.forename: Qide
authors.initials: Q
authors.lastname: Lin
mesh_terms: D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: C106628:KISS1 protein, human; D059648:Kisspeptins; D012333:RNA, Messenger; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9