title: Efficient use of retention time for the analysis of 302 drugs in equine plasma by liquid chromatography-MS/MS with scheduled multiple reaction monitoring and instant library searching for doping control.
abstract: Multiple drug target analysis (MDTA) used in doping control is more efficient than single drug target analysis (SDTA). The number of drugs with the potential for abuse is so extensive that full coverage is not possible with SDTA. To address this problem, a liquid chromatography tandem mass spectrometric method was developed for simultaneous analysis of 302 drugs using a scheduled multiple reaction monitoring (s-MRM) algorithm. With a known retention time of an analyte, the s-MRM algorithm monitors each MRM transition only around its expected retention time. Analytes were recovered from plasma by liquid-liquid extraction. Information-dependent acquisition (IDA) functionality was used to combine s-MRM with enhanced product ion (EPI) scans within the same chromatographic analysis. An EPI spectrum library was also generated for rapid identification of analytes. Analysis time for the 302 drugs was 7 min. Scheduled MRM improved the quality of the chromatograms, signal response, reproducibility, and enhanced signal-to-noise ratio (S/N), resulting in more data points. Reduction in total cycle time from 2.4 s in conventional MRM (c-MRM) to 1 s in s-MRM allowed completion of the EPI scan at the same time. The speed for screening and identification of multiple drugs in equine plasma for doping control analysis was greatly improved by this method.
authors.affiliation: University of Pennsylvania School of Veterinary Medicine, New Bolton Center Campus, 382 West Street Road, Kennett Square, Pennsylvania 19348, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Cornelius E
authors.initials: CE
authors.lastname: Uboh
authors.forename: Lawrence R
authors.initials: LR
authors.lastname: Soma
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Li
authors.forename: Fuyu
authors.initials: F
authors.lastname: Guan
authors.forename: Youwen
authors.initials: Y
authors.lastname: You
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Chen
mesh_terms: D000465:Algorithms; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004300:Doping in Sports / Q000379:methods*; D006736:Horses; D007477:Ions / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000097:blood*; D059629:Signal-To-Noise Ratio; D015813:Substance Abuse Detection / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007477:Ions; D004364:Pharmaceutical Preparations