title: Lactobacillus paracasei reduces intestinal inflammation in adoptive transfer mouse model of experimental colitis.
abstract: Studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease. In vitro evidence suggested that Lactobacillus paracasei also called ST11 (CNCM I-2116) is a potent strain with immune modulation properties. However, little is known about its capacity to alleviate inflammatory symptoms in vivo In this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis. Rag2(-/-) recipient mice were fed with ST11 (10(9) CFU/day)a month prior toinduce colitis by adoptive transfer of naive T cells. One month later, in clear contrast to nonfed mice, weight loss was significantly reduced by 50% in ST11-fed mice. Further analysis of colon specimens revealed a significant reduction neutrophil infiltration and mucosal expression of IL1β, IL-6, and IL12 proinflammatory cytokines, whereas no consistent differences in expression of antibacterial peptides or tight junction proteins were observed between PBS and ST11-fed mice. All together, our results demonstrate that oral administration of ST11 was safe and had a significant preventive effect on colitis. We conclude that probiotics such as Lactobacillus paracasei harbor worthwhile in vivo immunomodulatory properties to prevent intestinal inflammation by nutritional approaches.
authors.affiliation: Nutrition and Health Department, Nestlé Research Center, CH 1000 Lausanne, Switzerland.
authors.forename: Manuel
authors.initials: M
authors.lastname: Oliveira
authors.forename: Nabil
authors.initials: N
authors.lastname: Bosco
authors.forename: Genevieve
authors.initials: G
authors.lastname: Perruisseau
authors.forename: Jeanne
authors.initials: J
authors.lastname: Nicolas
authors.forename: Iris
authors.initials: I
authors.lastname: Segura-Roggero
authors.forename: Stéphane
authors.initials: S
authors.lastname: Duboux
authors.forename: Muriel
authors.initials: M
authors.lastname: Briand
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Blum
authors.forename: Jalil
authors.initials: J
authors.lastname: Benyacoub
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000637:transplantation; D003092:Colitis / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D004268:DNA-Binding Proteins / Q000172:deficiency; D004195:Disease Models, Animal; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D007778:Lactobacillus / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000627:therapeutic use*
chemical_list: D004268:DNA-Binding Proteins; C499308:Rag2 protein, mouse