title: Antiparasitic activity of C-geranyl flavonoids from Mimulus bigelovii.
abstract: Bioactivity-directed fractionation of the MeOH fraction of the extract of Mimulus bigelovii by means of an axenic Leishmania amastigote assay and chromatographic techniques resulted in the isolation of four C-geranyl flavanones, diplacone (1), 3'-O-methyldiplacone (2), 4'-O-methyldiplacone (3), 3'-O-methyldiplacol (4), together with a geranylated flavone, cannflavin A (5). These compounds were separated from M. bigelovii for the first time. All compounds showed moderate antileishmanial activity against axenic Leishmania donovani amastigotes with IC(50) values ranging from 4.8 to 14.6 μg/mL. The compounds were also tested against the related kinetoplastid parasite Trypanosoma brucei brucei and they showed activity with IC(50) values ranging from 1.4 to 7.2 μg/mL.
authors.affiliation: Department of Pharmacognosy, Tanta University, Egypt. salem.17@osu.edu
authors.forename: Manar M
authors.initials: MM
authors.lastname: Salem
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Capers
authors.forename: Shawn
authors.initials: S
authors.lastname: Rito
authors.forename: Karl A
authors.initials: KA
authors.lastname: Werbovetz
mesh_terms: D000981:Antiprotozoal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002140:California; D044950:Flavanones / Q000494:pharmacology; D047309:Flavones / Q000494:pharmacology; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D007893:Leishmania donovani / Q000187:drug effects*; D032241:Mimulus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D044950:Flavanones; D047309:Flavones; D005419:Flavonoids; D010936:Plant Extracts