title: [Designing of hybrid human interferon alfa-2 strain-producers and the use of enteropeptidase for obtaining N-terminal methionine-free interferons].
abstract: A system for production of human interferon-alpha2a (IFN-alpha2a) and IFN-alpha2b lacking N-terminal methionine has been developed. Plasmids containing genes of hybrid IFN-alpha2 under the control of different promoters were constructed; a sequence encoding the enteropeptidase hydrolysis site being introduced in proximal part of the genes. As the result, 4 strains of Escherichia coli producing hybrid IFN-alpha2 have been obtained. The methodology for IFN-alpha2 renaturation, hydrolysis of its N-terminal part, chromatographic purification of N-terminal methionine-free IFN-alpha2 has been developed.
authors.forename: D A
authors.initials: DA
authors.lastname: Shirokov
authors.forename: V V
authors.initials: VV
authors.lastname: Riabichenko
authors.forename: R I
authors.initials: RI
authors.lastname: Akishina
authors.forename: T P
authors.initials: TP
authors.lastname: Ospel'nikova
authors.forename: A V
authors.initials: AV
authors.lastname: Glazunov
authors.forename: G G
authors.initials: GG
authors.lastname: Chestukhina
authors.forename: V P
authors.initials: VP
authors.lastname: Veĭko
mesh_terms: D004765:Enteropeptidase / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D008715:Methionine / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D020673:Protein Renaturation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D011994:Recombinant Proteins
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D008715:Methionine; D004765:Enteropeptidase