title: Development of a cholesterol-conjugated fluorescent sensor for site-specific detection of zinc ion at the plasma membrane.
abstract: A cholesterol-conjugated fluorescence Zn(2+) sensor based on the fluorescein platform was designed and synthesized. The cholesterol moiety is essential for localizing the Zn(2+) sensor to the cell membrane, allowing the sensor to probe changes in the Zn(2+) concentration in a localized area of the cell.
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Iyoshi
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taki
authors.forename: Yukio
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D002462:Cell Membrane / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D015394:Molecular Structure; D013237:Stereoisomerism; D015032:Zinc / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D002784:Cholesterol; D015032:Zinc