title: Tumor-selective, adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection.
abstract: We have previously developed a telomerase-specific replicating adenovirus expressing GFP (OBP-401), which can selectively label tumors in vivo with GFP. Intraperitoneal (i.p.) injection of OBP-401 specifically labeled peritoneal tumors with GFP, enabling fluorescence visualization of the disseminated disease and real-time fluorescence surgical navigation. However, the technical problems with removing all cancer cells still remain, even with fluorescence-guided surgery. In this study, we report imaging of tumor recurrence after fluorescence-guided surgery of tumors labeled in vivo with the telomerase-dependent, GFP-containing adenovirus OBP-401.. Recurrent tumor nodules brightly expressed GFP, indicating that initial OBP-401-GFP labeling of peritoneal disease was genetically stable, such that proliferating residual cancer cells still express GFP. In situ tumor labeling with a genetic reporter has important advantages over antibody and other non-genetic labeling of tumors, since residual disease remains labeled during recurrence and can be further resected under fluorescence guidance.
authors.affiliation: AntiCancer, Inc., San Diego, CA, USA.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishimoto
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Aki
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Urata
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.forename: Masashi
authors.initials: M
authors.lastname: Momiyama
authors.forename: Noriaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Fujiwara
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000276:immunology; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology; D012008:Recurrence
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D049452:Green Fluorescent Proteins