title: Wide-field high-resolution structured illumination solid immersion fluorescence microscopy.
abstract: The use of aplanatic solid immersion lenses (ASILs) made of high-refractive-index optical materials provides a route to wide-field high-resolution optical microscopy. Structured illumination microscopy (SIM) can double the spatial bandwidth of a microscope to also achieve high-resolution imaging. We investigate the combination of ASILs and SIM in fluorescence microscopy, which we call structured illumination solid immersion fluorescence microscopy (SISIM), to pursue a microscopic system with very large NA and high lateral resolution. We demonstrate that the combination can produce a wide-field high-resolution microscopic system with bandwidth corresponding to an NA of 3. Future developments of the SISIM system to make it achieve even higher resolution are proposed.
authors.affiliation: Institute of Biophysics Imaging and Optical Science, The University of Nottingham, University Park, Nottingham NG7 2RD, UK.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Mark C
authors.initials: MC
authors.lastname: Pitter
authors.forename: Michael G
authors.initials: MG
authors.lastname: Somekh
mesh_terms: D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D008863:Microspheres