title: Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water.
abstract: Three enterococcal isolates, CCRI-16620, CCRI-16986(T) and CCRI-16985(T), originating from water were characterized using morphological, biochemical and molecular taxonomic methods. 16S rRNA gene sequence analysis classified all three strains in the Enterococcus faecalis species group. The phylogenetic tree of 16S rRNA gene sequences showed that the three isolates form two separate branches. The first branch is represented by strains CCRI-16620 and CCRI-16986(T) and the second branch by strain CCRI-16985(T). Further sequence analysis of the housekeeping genes rpoA (encoding RNA polymerase α subunit), pheS (phenylalanyl-tRNA synthase), tufA (elongation factor Tu) and atpD (ATP synthase β-subunit) as well as the results of amplified fragment length polymorphism (AFLP) DNA fingerprinting and DNA-DNA hybridization experiments confirmed the distinct status of these strains. Moreover, biochemical tests allowed phenotypic differentiation of the strains from the other species of the E. faecalis species group. On the basis of the results obtained, the names Enterococcus ureasiticus sp. nov. (type strain CCRI-16986(T) = CCUG 59304(T) = DSM 23328(T) = LMG 26304(T)) and Enterococcus quebecensis sp. nov. (type strain CCRI-16985(T) = CCUG 59306(T) = DSM 23327(T) = LMG 26306(T)) are proposed for the two hitherto undescribed species.
authors.affiliation: Département de microbiologie-infectiologie et d'immunologie, Faculté de médecine, Université Laval, Québec, QC, Canada.|Centre de recherche en infectiologie de l'Université Laval, Centre de recherche du CHUQ, Pavillon CHUL, 2705 boulevard Laurier, RC-709, Québec, QC, G1V 4G2, Canada.
authors.forename: Viridiana
authors.initials: V
authors.lastname: Sistek
authors.affiliation: Département de microbiologie-infectiologie et d'immunologie, Faculté de médecine, Université Laval, Québec, QC, Canada.|Centre de recherche en infectiologie de l'Université Laval, Centre de recherche du CHUQ, Pavillon CHUL, 2705 boulevard Laurier, RC-709, Québec, QC, G1V 4G2, Canada.
authors.forename: Andrée F
authors.initials: AF
authors.lastname: Maheux
authors.affiliation: Département de microbiologie-infectiologie et d'immunologie, Faculté de médecine, Université Laval, Québec, QC, Canada.|Centre de recherche en infectiologie de l'Université Laval, Centre de recherche du CHUQ, Pavillon CHUL, 2705 boulevard Laurier, RC-709, Québec, QC, G1V 4G2, Canada.
authors.forename: Maurice
authors.initials: M
authors.lastname: Boissinot
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.|National Microbiology Laboratory, Health Canada H5040-1015 Arlington St, Winnipeg, MB, Canada.
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Bernard
authors.affiliation: Centre d'Expertise en Analyse Environnementale du Québec, Ministère du Développement Durable de l'Environnement et des Parcs, Québec, QC, Canada.
authors.forename: Philippe
authors.initials: P
authors.lastname: Cantin
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.affiliation: LM-UGent, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.|BCCM/LMG Bacteria Collection, Ghent University, Ghent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
authors.affiliation: Département de microbiologie-infectiologie et d'immunologie, Faculté de médecine, Université Laval, Québec, QC, Canada.|Centre de recherche en infectiologie de l'Université Laval, Centre de recherche du CHUQ, Pavillon CHUL, 2705 boulevard Laurier, RC-709, Québec, QC, G1V 4G2, Canada.
authors.forename: Michel G
authors.initials: MG
authors.lastname: Bergeron
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S