title: [DNA methylation profile in human placental tissues].
abstract: For the first time using genome-wide Infinium HumanMethylation27 BeadChip Array (Illumina, USA) DNA methylation pattern was determined in the human cytotrophoblast and extraembryonic mesoderm. These tissues are the derivatives of trophectoderm and inner cell mass of blastocyst and have substantial differences in dynamics of epigenetic genome reprogramming during early stages of differentiation. The genome of the extraembryonic mesoderm cells has been shown to be more methylated compared to the cytotrophoblast similarly to other mammalian species. Differences in methylation pattern of single CpG-dinucleotides and dinucleotides localized within promoter CpG-islands have been found. It has been shown that the majority of single CpG-dinucleotides in both tissues were methylated whereas promoter CpG-island sites were not. Comparative analysis revealed 202 differentially methylated genes in extraembryonic mesoderm and 40 genes in cytotrophoblast. These genes are responsible for diverse biological processes. However, in the extraembryonic mesoderm the main functional groups included genes responsible for DNA binding and transcriptional factors' activity whereas in the cytotrophoblast--for transport and protein and cytokine secretion.
authors.forename: E N
authors.initials: EN
authors.lastname: Tolmacheva
authors.forename: A A
authors.initials: AA
authors.lastname: Kashevarova
authors.forename: N A
authors.initials: NA
authors.lastname: Skriabin
authors.forename: I N
authors.initials: IN
authors.lastname: Lebedev
mesh_terms: D001755:Blastocyst / Q000378:metabolism; D018899:CpG Islands; D019175:DNA Methylation; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008648:Mesoderm / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D014327:Trophoblasts / Q000378:metabolism*