title: Impact of filaggrin mutations on Raman spectra and biophysical properties of the stratum corneum in mild to moderate atopic dermatitis.
abstract: BACKGROUND
  Atopic dermatitis (AD) is associated with null mutations in the filaggrin (FLG) gene.
  
  
  OBJECTIVE
  To assess the impact of FLG null mutations on biophysical properties and the molecular composition of the stratum corneum (SC) in healthy individuals and AD patients.
  
  
  METHODS
  A total of 196 French adults, including 97 with a history of mild to moderate AD, were genotyped for the three major European FLG mutations. Components of the natural moisturizing factor (NMF), lipids and water content in the SC were determined using Raman spectroscopy. In addition, trans-epidermal water loss, capacitance and pH of the SC were measured.
  
  
  RESULTS
  Stratum corneum concentrations of total NMF, water, ornithine and urocanic acid (UCA) were significantly lower in AD patients than in healthy controls. Null mutations of FLG were detected in 4% of controls and 10% of AD patients. FLG mutations were associated with increased SC levels of lactate, reduced concentrations of most other NMF components and higher disease severity in AD patients. In AD patients without FLG mutations, the content of NMF constituents decreased with increasing disease severity. The concomittant presence of low concentrations of histidine, alanine and either glycine or pyrrolidone-5-carboxylic acid (PCA) in the SC was associated with FLG mutations with 92% specificity.
  
  
  CONCLUSIONS
  Our findings suggest a low prevalence of FLG mutations in mild AD and support an important role for filaggrin in determining the physicochemical parameters of the SC. The combined measurement of several filaggrin breakdown products in the SC may be useful to specifically predict the presence of FLG mutations.
authors.affiliation: Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors.forename: V
authors.initials: V
authors.lastname: Mlitz
authors.forename: J
authors.initials: J
authors.lastname: Latreille
authors.forename: S
authors.initials: S
authors.lastname: Gardinier
authors.forename: R
authors.initials: R
authors.lastname: Jdid
authors.forename: Y
authors.initials: Y
authors.lastname: Drouault
authors.forename: P
authors.initials: P
authors.lastname: Hufnagl
authors.forename: L
authors.initials: L
authors.lastname: Eckhart
authors.forename: C
authors.initials: C
authors.lastname: Guinot
authors.forename: E
authors.initials: E
authors.lastname: Tschachler
mesh_terms: D000328:Adult; D001483:Base Sequence; D001703:Biophysics; D016022:Case-Control Studies; D017931:DNA Primers; D003876:Dermatitis, Atopic / Q000235:genetics / Q000473:pathology*; D004817:Epidermis / Q000473:pathology*; D005260:Female; D000091344:Filaggrin Proteins; D005838:Genotype; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008297:Male; D009154:Mutation; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D017931:DNA Primers; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins