title: Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens.
abstract: The notion to fight against multi drug resistant pathogens is a great deal in the field of nanomedicine. The identifiable antimicrobial action of metal bionanoparticles on many microorganisms is reported earlier. As silver bionanoparticles (Ag-BNPs) are known to have efficient antibacterial properties they are synthesized in ecofriendly and biocompatible way. The present study is focused on the extracellular biosynthesis of highly stable Ag-BNPs from bacterial strain Bacillus megaterium (NCIM 2326) by bio-reduction of silver ion using the culture supernatant, and to determine the antibacterial efficacy on multi drug resistant clinical pathogens such as Streptococcus pneumoniae and Salmonella typhi. The biosynthesis process is rapid and Ag-BNPs are formed within few minutes if AgNO(3) comes to contact with cell filtrate. Furthermore the synthesized Ag-BNPs are characterized by UV-vis spectroscopy, Atomic Force Microscopy (AFM), Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR).
authors.affiliation: Department of Biotechnology, Faculty of Science and Humanities, SRM University, SRM Nagar, Kattankulathur, Chennai, Tamilnadu, India. bioinfosaran@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Saravanan
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Vemu
authors.forename: Sisir Kumar
authors.initials: SK
authors.lastname: Barik
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D012485:Salmonella typhi / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013296:Streptococcus pneumoniae / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D012835:Silver Nitrate