title: Cryptococcus cyanovorans sp. nov., a basidiomycetous yeast isolated from cyanide-contaminated soil.
abstract: Eighteen yeast strains were isolated and identified from cyanide-contaminated soil in South Africa. According to sequence-based analyses using the D1/D2 region of the large ribosomal subunit and ITS region, three of these strains were found to be identical and represent a novel species. Phylogenetic analysis based on the combined dataset of the D1/D2 and ITS regions revealed a grouping with Cryptococcus curvatus, representing a defined clade (Curvatus) in the order Trichosporonales. The three strains were demarcated from Cryptococcus curvatus by standard physiological tests such as assimilation of lactose, xylitol, 5-keto-D-gluconate, succinate and citrate as well as growth on media containing 10 % (w/v) NaCl and 5 % (w/v) glucose. In addition, it was established that these strains could utilize up to 10 mM NaCN as sole carbon source on solid media and as sole nitrogen source in liquid media. On the basis of these findings, it is suggested that the three strains represent a novel species for which the name Cryptococcus cyanovorans sp. nov. is given (type strain CBS 11948(T) = NRRL Y-48730(T)).
authors.affiliation: Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
authors.forename: Thabiso E
authors.initials: TE
authors.lastname: Motaung
authors.affiliation: Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
authors.forename: Jacobus
authors.initials: J
authors.lastname: Albertyn
authors.affiliation: Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
authors.forename: Johan L F
authors.initials: JLF
authors.lastname: Kock
authors.affiliation: Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa.
authors.forename: Carolina H
authors.initials: CH
authors.lastname: Pohl
mesh_terms: D002244:Carbon / Q000378:metabolism; D016000:Cluster Analysis; D003454:Cryptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003470:Culture Media / Q000737:chemistry; D003486:Cyanides / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D013019:South Africa
chemical_list: D003470:Culture Media; D003486:Cyanides; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012331:RNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S; D012989:Soil Pollutants; D002244:Carbon; D009584:Nitrogen