title: SIRT1 and SIRT5 activity expression and behavioral responses to calorie restriction.
abstract: To investigate the effects of calorie restriction (CR) on behavioral performance and expression of SIRT1 and SIRT5 in rat cerebral tissues. Beginning at 18 months of age, 60 rats were randomly divided into a CR group (n = 30) and a group that remained fed ad libitum (AL; n = 30). CR rats were restricted to a diet of 60% of their daily food consumption. After 6 months of CR, CR rats displayed a maximum 50% reduction in escape latency (AL 20 ± 0.3 s vs. CR 10 ± 0.2 s) and a 3.2 s decrease in time and distance to target when evaluated in Morris water maze tests. The levels of SIRT1 and SIRT5 protein in cerebral tissues of CR rats were elevated compared to AL rats (P < 0.05). CR retarded declines in cognitive ability and enhanced the expression of both SIRT1 and SIRT5 proteins in the cerebral tissue of CR rats compared with AL rats.
authors.affiliation: Central Laboratory, Shantou University and The Chinese University of Hong Kong Joint Shantou International Eye Center, Shantou, Guangdong 515041, China.
authors.forename: Yi-Qun
authors.initials: YQ
authors.lastname: Geng
authors.forename: Tian-Ti
authors.initials: TT
authors.lastname: Li
authors.forename: Xiang-Yu
authors.initials: XY
authors.lastname: Liu
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: Fu
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D031204:Caloric Restriction; D005455:Fluorescent Antibody Technique; D008297:Male; D018782:Maze Learning; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000378:metabolism*; D037761:Sirtuins / Q000378:metabolism*
chemical_list: C530376:SIRT5 protein, rat; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; D037761:Sirtuins