title: Comparison of real-time reverse-transcription loop-mediated isothermal amplification and real-time reverse-transcription polymerase chain reaction for detection of noroviruses in municipal wastewater.
abstract: The monitoring of NVs in municipal wastewater by both real-time RT-LAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1<p) for both NV-GI and NV-GII.
authors.affiliation: Department of Civil and Environmental Engineering, University of Miyazaki, Miyazaki 889-2192, Japan. suzuki@civil.miyazaki-u.ac.jp
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Shogo
authors.initials: S
authors.lastname: Narimatsu
authors.forename: Takashi
authors.initials: T
authors.lastname: Furukawa
authors.forename: Akira
authors.initials: A
authors.lastname: Iwakiri
authors.forename: Miho
authors.initials: M
authors.lastname: Miura
authors.forename: Shogo
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katayama
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
chemical_list: D012367:RNA, Viral