title: Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae.
abstract: Rous sarcoma virus group antigen protein-based virus-like particles (VLPs) are well known for their structural integrity and ease of handling. VLPs play an important role in drug delivery systems because they can be manipulated with ease. In this study, a new method was established for expressing Rous sarcoma virus group antigen protein based VLPs in silkworm larvae and establishing stably expressing insect cell lines. These VLPs have been isolated by ultracentrifugation using a sucrose step gradient of 10-60% (v/v), and their spherical structure has been confirmed using transmission electron microscopy (TEM). The spherical morphology is similar in both the silkworm larvae and in stably expressing cell lines. Silkworm larvae are better suited for producing Rous sarcoma virus group antigen protein-based VLPs on a large scale; yields from silkworm larvae were approximately 8.2-fold higher than yields from stable cell lines. These VLPs provide a new method for large-scale application in vaccine development and drug delivery systems.
authors.affiliation: Laboratory of Biotechnology, Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Vipin Kumar
authors.initials: VK
authors.lastname: Deo
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Tsuji
authors.forename: Tomomi
authors.initials: T
authors.lastname: Yasuda
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kato
authors.forename: Naonori
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Hisao
authors.initials: H
authors.lastname: Suzuki
authors.forename: Enoch Y
authors.initials: EY
authors.lastname: Park
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D015153:Blotting, Western; D012831:Bombyx / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D015683:Gene Products, gag / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D049750:Genome, Insect; D006458:Hemolymph / Q000378:metabolism; D007814:Larva / Q000235:genetics / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D052736:Rous sarcoma virus / Q000235:genetics / Q000378:metabolism*; D014461:Ultracentrifugation; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000956:Antigens, Viral; D015683:Gene Products, gag; D014764:Viral Proteins