title: [Effect of different concentration of tamoxifen ointment on the expression of TGF-beta2 of hypertrophic scar at rabbit ears].
abstract: OBJECTIVE
  To observe the effect of different concentration of Tamoxifen ointment on the fibroblasts and transforming growth factor (TGF-beta2) of hypertrophic scar at rabbit ears, so as to explore the possibility of treatment of hypertrophic scar with Tamoxifen.
  
  
  METHODS
  The hypertrophic scar model was established in 96 New Zealand rabbits' ears. The wounds were divided into four groups (A, B, C and D), with 144 wounds in each group. Different concentration of tamoxifen ointment (0.5%, 1%, 2%) was topically administered in groups A, B and C respectively, and blank ointment in group D. On postoperative day 30, 60 and 90, the scar samples were harvested. The scar thickness, scar histological change and the content of TGF-beta2 were detected.
  
  
  RESULTS
  (1) On the 30th day after operation, the difference of scar tissue thickness among groups A, D and B, C reached statistical significance (group A, D < group B < group C). However, there was a contrary tendency in fibroblasts density and TGF-beta2 content of the scar tissue simultaneously. (2) On 60th, 90th day after injury, there was statistical difference in scar thickness, fibroblasts density and the content of TGF-beta2 in scar of four groups (P < 0.05). The content of TGF-beta2 in group A, B, C, D was (43.97 +/- 3.63) microg/L, (41.92 +/- 3.91) microg/L, (36.69 +/- 4.15) microg/L, (54.90 +/- 4.71) microg/L, respectively, on 60th day; and (45.69 +/- 2.63) microg/L, (40.43 +/- 3.87) microg/L, (38.76 +/- 3.24) microg/L, (52.59 +/- 4.92) microg/L, respectively, on 90th day. The fibroblasts density of scar in groups A, B, C, D was (4392.07 +/- 327.84) point/mm2, (4208.57 +/- 329.76) point/mm2 (4 033.44 +/- 427.91) point/mm2, (4863.03 +/- 387.98) point/mm2, respectively, on 60th day; and (4418.41 +/- 432.52) point/mm2, (4077.65 +/- 386.70) point/mm2, (3844.53 +/- 354.29) point/mm2, (4838.64 +/- 390.52) point/mm2, respectively, on 90th day. The content of TGF-beta2 and fibroblasts density of scar were lined up as group D > group A > group B > group C (P < 0.05).
  
  
  CONCLUSIONS
  Topical Tamoxifen can reduce the content of TGF-beta2 and fibroblast, decrease fibroblasts density and the formation of hypertrophic scar at rabbit ears. It offers a new way for the treatment of the hypertrophic scar.
authors.affiliation: Department of Burn and Plastic Surgery, First Affiliated Hospital of General Hospital of PLA, Beijing 100048, China.
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Zhao
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Hui-feng
authors.initials: HF
authors.lastname: Song
authors.forename: Yan-fu
authors.initials: YF
authors.lastname: Han
authors.forename: Ming-huo
authors.initials: MH
authors.lastname: Xu
authors.forename: Tian-jun
authors.initials: TJ
authors.lastname: Sun
authors.forename: Dong-jie
authors.initials: DJ
authors.lastname: Li
mesh_terms: D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004427:Ear Diseases / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000473:pathology; D009824:Ointments; D011817:Rabbits; D013629:Tamoxifen / Q000494:pharmacology*; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*
chemical_list: D009824:Ointments; D053781:Transforming Growth Factor beta2; D013629:Tamoxifen