title: Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales).
abstract: Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC₅₀ of 4.1 and 17.2 μg/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and potential virucidal effect. The polysaccharides from S. coronopifolius (PSC) and B. thuyoides (PBT) were composed of galactose, 3,6-anhydrogalactose, uronics acids, sulfate in ratios of 33.1, 11.0, 7.7 and 24.0% (w/w) and 25.4, 16.0, 3.2, 7.6% (w/w), respectively.
authors.affiliation: Laboratoire de Biotechnologie et Chimie Marines (LBCM), Centre de recherche Yves Coppens, Université européenne de Bretagne (UEB), Université de Bretagne-Sud. Vannes, France.|Laboratoire de Diversité et Conservation de Systèmes Biologiques (LDICOSYB), Faculté de Science, Université Abdelmalek Essaâdi, Tétouan, Maroc. B.P. 2121, 93003, Morocco.
authors.forename: Rhimou
authors.initials: R
authors.lastname: Bouhlal
authors.affiliation: URRMA R&D, Centre de vie agora, bâtiment C, B.P. 1055, Z.I. des paluds, 13781 Aubagne Cedex, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Haslin
authors.affiliation: URRMA R&D, Centre de vie agora, bâtiment C, B.P. 1055, Z.I. des paluds, 13781 Aubagne Cedex, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chermann
authors.affiliation: Laboratoire Biotechnologies et Molécules Marines, IFREMER, B.P. 21105 44311 Nantes, France.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Colliec-Jouault
authors.affiliation: Laboratoire Biotechnologies et Molécules Marines, IFREMER, B.P. 21105 44311 Nantes, France.
authors.forename: Corinne
authors.identifier: 0000-0002-8930-9535
authors.initials: C
authors.lastname: Sinquin
authors.affiliation: Université européenne de Bretagne, Université de Bretagne Occidentale, Service Commun de Résonance Magnétique Nucléaire, UFR Sciences et Techniques, Avenue Le Gorgeu, CS 93837 29238 Brest Cedex 3, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Simon
authors.affiliation: Université européenne de Bretagne, Université de Bretagne Occidentale, Service Commun de Résonance Magnétique Nucléaire, UFR Sciences et Techniques, Avenue Le Gorgeu, CS 93837 29238 Brest Cedex 3, France.
authors.forename: Stephane
authors.initials: S
authors.lastname: Cerantola
authors.affiliation: Laboratoire de Diversité et Conservation de Systèmes Biologiques (LDICOSYB), Faculté de Science, Université Abdelmalek Essaâdi, Tétouan, Maroc. B.P. 2121, 93003, Morocco.
authors.forename: Hassane
authors.initials: H
authors.lastname: Riadi
authors.affiliation: Laboratoire de Biotechnologie et Chimie Marines (LBCM), Centre de recherche Yves Coppens, Université européenne de Bretagne (UEB), Université de Bretagne-Sud. Vannes, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bourgougnon
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival; D002522:Chlorocebus aethiops; D005690:Galactose / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects; D018259:Herpesvirus 1, Human / Q000187:drug effects; D006801:Humans; D053515:Matrix Metalloproteinase 17 / Q000187:drug effects; D008970:Molecular Weight; D009018:Morocco; D009792:Oceans and Seas; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000461:Rhodophyta / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology; D013997:Time Factors; D014574:Uronic Acids / Q000737:chemistry / Q000494:pharmacology; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D028321:Plant Preparations; D011134:Polysaccharides; D013431:Sulfates; D014574:Uronic Acids; C117625:3,6-anhydrogalactose; D053515:Matrix Metalloproteinase 17; D005690:Galactose