title: Polyhydroxyalkanoate (PHA) synthesis by class IV PHA synthases employing Ralstonia eutropha PHB(-)4 as host strain.
abstract: Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T) (PhaRC(Bm)) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).
authors.affiliation: Department of Innovative and Engineered Materials, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Hyakutake
authors.forename: Yuta
authors.initials: Y
authors.lastname: Saito
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tomizawa
authors.forename: Kouhei
authors.initials: K
authors.lastname: Mizuno
authors.forename: Takeharu
authors.initials: T
authors.lastname: Tsuge
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism*; D001410:Bacillus megaterium / Q000235:genetics / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D020118:Cupriavidus necator / Q000235:genetics / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism; D010957:Plasmids; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity; D014169:Transformation, Bacterial
chemical_list: D010938:Plant Oils; D054813:Polyhydroxyalkanoates; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase