title: Compositional safety of event DAS-40278-9 (AAD-1) herbicide-tolerant maize.
abstract: Event DAS-40278-9 maize expresses the aryloxyalkanoate dioxygenase-1 enzyme, which was originally identified in the soil bacterium Sphingobium herbicidovorans. This enzyme degrades 2,4-dichlorophenoxyacetic acid (2,4-D) and aryloxyphenoxypropionate herbicides (e.g., haloxyfop, cyhalofop, quizalofop, etc.); therefore, plants that contain this enzyme are tolerant to these herbicides. We employed the substantial equivalence approach to investigate the compositional safety of event DAS-40278-9 maize. A total of 82 different compositional analyses were conducted to evaluate the equivalence of event DAS-40278-9 and conventional maize. Analyte levels within the transgenic entries were either within literature ranges for non-transgenic maize or statistically indistinguishable from the non-transgenic near-isogenic hybrid, thus indicating substantial equivalence between event DAS-40278-9 and its conventional counterpart. These results agree with dozens of published studies for other transgenic events where input traits were found to have a negligible effect on crop composition compared with traditional breeding methods.
authors.affiliation: Dow AgroSciences LLC, Indianapolis, IN, USA. raherman@dow.com
authors.forename: Rod A
authors.initials: RA
authors.lastname: Herman
authors.forename: Amy M
authors.initials: AM
authors.lastname: Phillips
authors.forename: Miles D
authors.initials: MD
authors.lastname: Lepping
authors.forename: Brandon J
authors.initials: BJ
authors.lastname: Fast
authors.forename: Jane
authors.initials: J
authors.lastname: Sabbatini
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology*; D030821:Plants, Genetically Modified / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D003313:Zea mays / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D006540:Herbicides; D015084:2,4-Dichlorophenoxyacetic Acid; D049308:Dioxygenases