title: [Simultanenous determination of contents of four nitrogenous compounds in Pinellia peda-tisecta by HPLC].
abstract: OBJECTIVE
  To develop a HPLC method for the quantitative determination of four nitrogenous compounds in Pinellia peda-tisecta.
  
  
  METHODS
  The determination was carried out on Diamonsil-C18 column(4. 6 mm x 250 mm, 5 microm) eluted with acetonitrile-water-triethylamine in gradient elution acetonitrile-water- triethylamine. The mobile phase was a flow rate of 1.0 mL x min(-1) and detection wavelength as 254 nm.
  
  
  RESULTS
  The linear ranges of trigonelline, thymine, amidopurine and hypoxanthine were 0.2 - 1.0 microg (r = 0.999 6), 2.6-13 microg (r = 0.999 8), 0.55-2.75 microg (r = 0.999 9), 0.65-3.25 microg (r = 0.999 8), respectively. The average recoveries of trigonelline, thymine, amidopurine and hypoxanthine were 100.6%, 99.89%,100.5% and 100.0% respectively, and the RSD of the above four compounds were 2.4%, 0.20%, 1.1%, 1.4%, respectively.
  
  
  CONCLUSIONS
  This method is reliable, accurate and specific. It can be used for quality control of the tube of P. peda-tisecta.
authors.affiliation: Chinese Medicine College, Beijing University of Chinese Medicine, Beijing 100102, China. xukaixk11@163.com
authors.forename: Lijun
authors.initials: L
authors.lastname: Xu
authors.forename: Shengli
authors.initials: S
authors.lastname: Wei
authors.forename: Wenquan
authors.initials: W
authors.lastname: Wang
authors.forename: Xueyan
authors.initials: X
authors.lastname: Gao
authors.forename: Junying
authors.initials: J
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification; D017672:Nitrogen Compounds / Q000032:analysis* / Q000302:isolation & purification; D030021:Pinellia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D017672:Nitrogen Compounds