title: Lack of association between IL-6 gene polymorphisms and rheumatoid arthritis in Turkish population.
abstract: Inflammatory cytokines play an important role in the pathogenesis of rheumatoid arthritis (RA). One of candidate genes is interleukin-6 (IL-6), and single-nucleotide polymorphisms in the promoter region of IL-6 were found to be associated with RA. The aim of this study was to determine the association between IL-6 promoter polymorphisms (-174, -572, -597) and RA in Turkish population. A total of 425 subjects were recruited into the study (247 healthy controls and 178 RA). The promoter region of IL-6 gene was amplified by PCR using DNAs from patients and the controls, and their PCR products were digested by suitable enzymes. No significant association was found between RA and -174 genotype distribution (P = 0.535) and allele frequency (P = 0.230). There was also no relationship between -572 (P = 0.150) and -597 (P = 0.912) gene polymorphism and RA. Our results suggested that IL-6 gene promoter polymorphisms have no association with RA in Turkish population.
authors.affiliation: The Faculty of Engineering, Molecular Genetics and Cell Culture Laboratory, Marmara University, Goztepe Campus, 34722 Istanbul, Turkey. ahmetarman@marmara.edu.tr
authors.forename: A
authors.initials: A
authors.lastname: Arman
authors.forename: A
authors.initials: A
authors.lastname: Coker
authors.forename: O
authors.initials: O
authors.lastname: Sarioz
authors.forename: N
authors.initials: N
authors.lastname: Inanc
authors.forename: H
authors.initials: H
authors.lastname: Direskeneli
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014421:Turkey / Q000453:epidemiology
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6