title: Fine tuning by human CD1e of lipid-specific immune responses.
abstract: CD1e is a member of the CD1 family that participates in lipid antigen presentation without interacting with the T-cell receptor. It binds lipids in lysosomes and facilitates processing of complex glycolipids, thus promoting editing of lipid antigens. We find that CD1e may positively or negatively affect lipid presentation by CD1b, CD1c, and CD1d. This effect is caused by the capacity of CD1e to facilitate rapid formation of CD1-lipid complexes, as shown for CD1d, and also to accelerate their turnover. Similar results were obtained with antigen-presenting cells from CD1e transgenic mice in which lipid complexes are assembled more efficiently and show faster turnover than in WT antigen-presenting cells. These effects maximize and temporally narrow CD1-restricted responses, as shown by reactivity to Sphingomonas paucimobilis-derived lipid antigens. CD1e is therefore an important modulator of both group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability as well as the generation and persistence of CD1-lipid complexes.
authors.affiliation: Experimental Immunology, Department of Biomedicine, University Hospital Basel, 4031 Basel, Switzerland.
authors.forename: Federica
authors.initials: F
authors.lastname: Facciotti
authors.forename: Marco
authors.initials: M
authors.lastname: Cavallari
authors.forename: Catherine
authors.initials: C
authors.lastname: Angénieux
authors.forename: Luis F
authors.initials: LF
authors.lastname: Garcia-Alles
authors.forename: François
authors.initials: F
authors.lastname: Signorino-Gelo
authors.forename: Lena
authors.initials: L
authors.lastname: Angman
authors.forename: Martine
authors.initials: M
authors.lastname: Gilleron
authors.forename: Jacques
authors.initials: J
authors.lastname: Prandi
authors.forename: Germain
authors.initials: G
authors.lastname: Puzo
authors.forename: Luigi
authors.initials: L
authors.lastname: Panza
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Xia
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Paolo
authors.initials: P
authors.lastname: Dellabona
authors.forename: Giulia
authors.initials: G
authors.lastname: Casorati
authors.forename: Steven A
authors.initials: SA
authors.lastname: Porcelli
authors.forename: Henri
authors.initials: H
authors.lastname: de la Salle
authors.forename: Lucia
authors.initials: L
authors.lastname: Mori
authors.forename: Gennaro
authors.initials: G
authors.lastname: De Libero
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D018949:Antigens, CD1 / Q000276:immunology*; D002999:Clone Cells; D003713:Dendritic Cells / Q000276:immunology; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology; D006801:Humans; D007109:Immunity / Q000276:immunology*; D007700:Kinetics; D008055:Lipids / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D055611:Natural Killer T-Cells / Q000276:immunology; D020578:Sphingomonas / Q000276:immunology
chemical_list: D018949:Antigens, CD1; C437944:CD1C protein, human; C419394:CD1b antigen; C417738:CD1e antigen; D006017:Glycolipids; D006023:Glycoproteins; D008055:Lipids