title: Gene silencing by siRNA microhydrogels via polymeric nanoscale condensation.
abstract: The first attempt to prepare biologically active siRNA-based microhydrogels is reported. The self-assembled microhydrogels were fabricated using sense/antisense complementary hybridization between single-stranded linear and Y-shaped trimeric siRNAs. The siRNA microhydrogels were condensed using a popular cationic polymer such as LPEI to form compact, stable siRNA/polymeric nanoparticles that exhibited superb cellular uptake efficiency and gene silencing activity.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
authors.forename: Cheol Am
authors.initials: CA
authors.lastname: Hong
authors.forename: Soo Hyeon
authors.initials: SH
authors.lastname: Lee
authors.forename: Jee Seon
authors.initials: JS
authors.lastname: Kim
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Park
authors.forename: Ki Hyun
authors.initials: KH
authors.lastname: Bae
authors.forename: Hyejung
authors.initials: H
authors.lastname: Mok
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Park
authors.forename: Haeshin
authors.initials: H
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D020100:Hydrogels; D011108:Polymers; D034741:RNA, Small Interfering