title: Simultaneous determination of efavirenz, rifampicin and its metabolite desacetyl rifampicin levels in human plasma.
abstract: A simple and rapid isocratic, high performance liquid chromatography (HPLC) assay employing solid phase extraction (SPE) for the simultaneous determination of the anti HIV drug, efavirenz, the anti-tuberculosis drug, rifampicin and the desacetyl metabolite of rifampicin in plasma from HIV/tuberculosis infected patients has been developed. Using a Zorbax SB-Phenyl reverse-phase analytical column with UV detection, good separation and detection of the drugs was attained within a 10min run time. Intra- and inter-assay precision RSD values were found to be less than 15% at the concentrations examined (0.1-20μg/mL). The LOQ was found to be 0.1μg/mL for each agent and the assay was found to generate a linear response up to 20μg/mL. This low cost assay can accurately detect efavirenz and rifampicin concentrations within a clinically relevant concentration range using standard chromatography equipment, making it particularly applicable to resource-limited settings.
authors.affiliation: School of Chemical Sciences, Dublin City University, Dublin 9, Ireland. Deirdre.fox4@mail.dcu.ie
authors.forename: Deirdre
authors.initials: D
authors.lastname: Fox
authors.forename: Robert
authors.initials: R
authors.lastname: O'Connor
authors.forename: Patrick
authors.initials: P
authors.lastname: Mallon
authors.forename: Gillian
authors.initials: G
authors.lastname: McMahon
mesh_terms: D000480:Alkynes; D019380:Anti-HIV Agents / Q000097:blood* / Q000592:standards / Q000627:therapeutic use; D000904:Antibiotics, Antitubercular / Q000097:blood* / Q000592:standards / Q000627:therapeutic use; D048588:Benzoxazines / Q000097:blood* / Q000592:standards / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003521:Cyclopropanes; D016903:Drug Monitoring; D004355:Drug Stability; D015658:HIV Infections / Q000188:drug therapy; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012293:Rifampin / Q000097:blood* / Q000592:standards / Q000627:therapeutic use; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000188:drug therapy
chemical_list: D000480:Alkynes; D019380:Anti-HIV Agents; D000904:Antibiotics, Antitubercular; D048588:Benzoxazines; D003521:Cyclopropanes; C098320:efavirenz; D012293:Rifampin