title: Solid respirometry to characterize nitrification kinetics: a better insight for modelling nitrogen conversion in vertical flow constructed wetlands.
abstract: We developed an original method to measure nitrification rates at different depths of a vertical flow constructed wetland (VFCW) with variable contents of organic matter (sludge, colonized gravel). The method was adapted for organic matter sampled in constructed wetland (sludge, colonized gravel) operated under partially saturated conditions and is based on respirometric principles. Measurements were performed on a reactor, containing a mixture of organic matter (sludge, colonized gravel) mixed with a bulking agent (wood), on which an ammonium-containing liquid was applied. The oxygen demand was determined from analysing oxygen concentration of the gas passing through the reactor with an on-line analyzer equipped with a paramagnetic detector. Within this paper we present the overall methodology, the factors influencing the measurement (sample volume, nature and concentration of the applied liquid, number of successive applications), and the robustness of the method. The combination of this new method with a mass balance approach also allowed determining the concentration and maximum growth rate of the autotrophic biomass in different layers of a VFCW. These latter parameters are essential inputs for the VFCW plant modelling.
authors.affiliation: Cemagref, UR MALY, 3 bis quai Chauveau-CP 220, F 69336 Lyon, France.
authors.forename: Ania
authors.initials: A
authors.lastname: Morvannou
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Choubert
authors.forename: Marnik
authors.initials: M
authors.lastname: Vanclooster
authors.forename: Pascal
authors.initials: P
authors.lastname: Molle
mesh_terms: D007700:Kinetics; D058465:Nitrification; D009584:Nitrogen / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D053833:Wetlands
chemical_list: D009584:Nitrogen; D010100:Oxygen