title: flaB gene as a molecular marker for distinct identification of Borrelia species in environmental samples by the PCR-restriction fragment length polymorphism method.
abstract: A new protocol employing nested PCR-restriction fragment length polymorphism (RFLP) based on the flaB gene and two restriction enzymes was worked out. This protocol allows the identification of all Borrelia species transmitted by Ixodes ricinus in Europe, including Borrelia miyamotoi and 3 genetic variants of B. garinii. A dendrogram of flaB sequence similarity was in accordance with RFLP variants.
authors.affiliation: Department of Genetics, Szczecin University, Felczaka 3c, 71-412 Szczecin, Poland. wodecka.us@gmail.com
authors.forename: Beata
authors.initials: B
authors.lastname: Wodecka
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D001898:Borrelia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016000:Cluster Analysis; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D005060:Europe; D005408:Flagellin / Q000235:genetics*; D018884:Ixodes / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005408:Flagellin; C416532:flaB flagellin; D004262:DNA Restriction Enzymes