title: Antiviral activity and mechanism of action of novel thiourea containing chiral phosphonate on tobacco mosaic virus.
abstract: Using half-leaf method O,O'-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K(326) (Nicotiana tabacum K(326)) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.
authors.affiliation: State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China; E-Mails: fanhuitao0818@163.com (H.F.); bhadury@gzu.edu.cn (B.P.); fcc.jinlh@gzu.edu.cn (L.J.); fcc.dyhu@gzu.edu.cn (D.H.).
authors.forename: Huitao
authors.initials: H
authors.lastname: Fan
authors.forename: Baoan
authors.initials: B
authors.lastname: Song
authors.forename: Pinaki S
authors.initials: PS
authors.lastname: Bhadury
authors.forename: Linhong
authors.initials: L
authors.lastname: Jin
authors.forename: Deyu
authors.initials: D
authors.lastname: Hu
authors.forename: Song
authors.initials: S
authors.lastname: Yang
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism; D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism; D003562:Cytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D063065:Organophosphonates / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000737:chemistry / Q000378:metabolism; D013237:Stereoisomerism; D013482:Superoxide Dismutase / Q000737:chemistry / Q000378:metabolism; D013890:Thiourea / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D014027:Tobacco Mosaic Virus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D036022:Capsid Proteins; C000594812:O,O'-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3-phenylpropan-2-yl)thioureido)(phenyl)methylphosphonate; D063065:Organophosphonates; C000594254:ningnanmycin; D002734:Chlorophyll; D003562:Cytidine; D009195:Peroxidase; D013482:Superoxide Dismutase; D010650:Phenylalanine Ammonia-Lyase; D013890:Thiourea