title: PAF increases phagocytic capacity and superoxide anion production in equine alveolar macrophages and blood neutrophils.
abstract: Phagocytosis exerted by alveolar macrophages and neutrophils is crucial in the clearance of exogenous particles deposited in the airways. Therefore, substances that activate these phagocytes in the airways can exert important effects on the particle clearance rate. PAF, particularly, was proved to be a potent activator of several immune cells and was shown to be present in the equine lower airways in specific conditions, such as after exercise. The present study aimed to investigate if PAF is able to increase the phagocytic capacity and the production of superoxide anion in equine alveolar macrophage and blood neutrophils. The results show that PAF increased these parameters in both phagocytes even in concentrations as low as 0.1 and 1.0 nM. On that ground, the present work suggests that PAF is involved in the process of particle clearance in equine lower airways.
authors.affiliation: Department of Genetics and Morphology, Instituto de Biologia, Campus Universitário Darcy Ribeiro, Universidade de Brasília, Brasília, DF 70910-900, Brazil. luismuehlmann@yahoo.de
authors.forename: Luis Alexandre
authors.initials: LA
authors.lastname: Muehlmann
authors.forename: Pedro Vicente
authors.initials: PV
authors.lastname: Michelotto
authors.forename: Everson Araújo
authors.initials: EA
authors.lastname: Nunes
authors.forename: Fernanda Cristine Ceccon
authors.initials: FC
authors.lastname: Grando
authors.forename: Fabiana Tieme
authors.initials: FT
authors.lastname: da Silva
authors.forename: Anita
authors.initials: A
authors.lastname: Nishiyama
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D006736:Horses; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*
chemical_list: D010972:Platelet Activating Factor; D013481:Superoxides