title: [Determination of reduced homocysteine fraction in blood plasma with HPLC-MS].
abstract: Homocysteine is a bioactive compound involved in many impotant processes of clinical significance. High sensitivity methods associated with preparation of oxidation-resistant derivatives are of demand for determination of different homocysteine fractions. In this work a derivatization agent N-ethylmaleinimide (NEM) was proposed by us for detection of reduced homocysteine fraction with HPLC-MS. Linearity, detection limit, and precision were 25-1500 nM, 10 nM, and 95-105% respectively, with reproducibility within 12%.
authors.forename: A V
authors.initials: AV
authors.lastname: Ivanov
authors.forename: B P
authors.initials: BP
authors.lastname: Luzianin
authors.forename: A A
authors.initials: AA
authors.lastname: Moskovtsev
authors.forename: A S
authors.initials: AS
authors.lastname: Rotkina
authors.forename: A A
authors.initials: AA
authors.lastname: Kubatiev
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006710:Homocysteine / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D006710:Homocysteine