title: Activated leukocyte cell adhesion molecule impacts on clinical wound healing and inhibits HaCaT migration.
abstract: Activated leukocyte cell adhesion molecule (ALCAM) is a glycoprotein of the immunoglobulin superfamily that has been implicated in the processes of cell adhesion and migration. The current study examines the importance of ALCAM in regulating HaCaT cell growth and migration and its potential to impact on wound healing. ALCAM levels were examined in a range of clinical wound and normal skin samples using Q-PCR and immunohistochemistry. ALCAM expression was targeted in HaCaT keratinocyte cells using a hammerhead ribozyme transgene system. Subsequently, the impact of ALCAM suppression on HaCaT migration and growth was assessed. ALCAM protein was detected mainly in keratinocytes. ALCAM transcript levels were found to be significantly higher in the non-healed chronic wound samples compared with healed samples (P = 0·026). In addition, targeting of ALCAM in HaCaT cells brought about a substantial increase in cellular migration and growth compared with HaCaT control cells.Our results suggest that ALCAM plays an important role in the migration of HaCaT keratinocyte cells. The data also suggests that higher levels of ALCAM may impair healing in chronic wounds. The impact of ALCAM in wound healing may thus be somewhat due to its impact on cell migration and growth.
authors.affiliation: Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Cardiff, UK. sandersaj1@cardiff.ac.uk
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Sanders
authors.forename: David G
authors.initials: DG
authors.lastname: Jiang
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
authors.forename: Keith G
authors.initials: KG
authors.lastname: Harding
authors.forename: Girish K
authors.initials: GK
authors.lastname: Patel
mesh_terms: D020410:Activated-Leukocyte Cell Adhesion Molecule / Q000096:biosynthesis / Q000235:genetics*; D001706:Biopsy; D002448:Cell Adhesion / Q000235:genetics*; D002465:Cell Movement; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D012313:RNA / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014647:Varicose Ulcer / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D020410:Activated-Leukocyte Cell Adhesion Molecule; D012313:RNA