title: The fabrication of a piezoelectric immunosensor based on DNA-antibody conjugate layer.
abstract: In this article, we report a method of antibody immobilization carried out by hybridizing DNA-antibody conjugates on a mixed self-assembled monolayer composed of DNA thiols and mercaptopropionic acid via sequence-specific hybridization. The proposed method was applied to fabricate an immunosensor for detecting human immunoglobulin G (IgG). Under the optimized experimental conditions, a wide linear range from 50.0 to 500 μg/ml was reached with a detection limit of 30.13 μg/ml. The developed immunosensor possesses advantages such as simple fabrication, wide linear range, easy regeneration, and excellent reproducibility.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde, Hunan 415000, China. sgyrab@yahoo.com.cn
authors.forename: Guangyu
authors.initials: G
authors.lastname: Shen
authors.forename: Jilin
authors.initials: J
authors.lastname: Lu
authors.forename: Chenbo
authors.initials: C
authors.lastname: Cai
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000276:immunology; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D018796:Immunoconjugates / Q000032:analysis / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D017403:In Situ Hybridization / Q000379:methods; D057230:Limit of Detection; D011791:Quartz; D015203:Reproducibility of Results; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D018796:Immunoconjugates; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D011791:Quartz; D006046:Gold; D004247:DNA; D015097:3-Mercaptopropionic Acid