title: Strategy for the extraction of yeast DNA from artisan agave must for quantitative PCR analysis.
abstract: An efficient method for the direct extraction of yeast genomic DNA from agave must was developed. The optimized protocol, which was based on silica-adsorption of DNA on microcolumns, included an enzymatic cell wall degradation step followed by prolonged lysis with hot detergent. The resulting extracts were suitable templates for subsequent qPCR assays that quantified mixed yeast populations in artisan Mexican mezcal fermentations.
authors.affiliation: Industrial Biotechnology Division, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco AC, Guadalajara 44270, Jalisco, Mexico.
authors.forename: Manuel Reinhart
authors.initials: MR
authors.lastname: Kirchmayr
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Segura-Garcia
authors.forename: Ericka Patricia
authors.initials: EP
authors.lastname: Flores-Berrios
authors.forename: Anne
authors.initials: A
authors.lastname: Gschaedler
mesh_terms: D027882:Agave / Q000382:microbiology*; D004271:DNA, Fungal / Q000235:genetics*; D005285:Fermentation; D005516:Food Microbiology / Q000379:methods*; D008800:Mexico; D016133:Polymerase Chain Reaction; D015003:Yeasts / Q000235:genetics*
chemical_list: D004271:DNA, Fungal