title: Ginsenoside F1 production from ginsenoside Rg1 by a purified β-glucosidase from Fusarium moniliforme var. subglutinans.
abstract: Fusarium moniliforme var. subglutinans was selected from among 100 strains of fungi for producing ginsenoside F(1) from ginsenoside Rg(1). The enzyme responsible was purified as a single 85 kDa band with a specific activity of 136 U mg(-1). It hydrolysed glucose-linked ginsenosides Rb(1), Rd and Rg(1) but not for other monosaccharide-linked ginsenosides, Rb(2), Rc, R(1), and Re. Under the optimum conditions of pH 6.0, 50 °C, 30 U l(-1) of enzyme, and 5 mg Rg(1) ml(-1), 4 mg F(1) ml(-1) was produced after 4 h, with a molar yield of 100% and a productivity of 1 g l(-1) h(-1). This represents the highest productivity and conversion yield of F(1) yet reported.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Yeong-Su
authors.initials: YS
authors.lastname: Kim
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Yoo
authors.forename: Gi-Woong
authors.initials: GW
authors.lastname: Lee
authors.forename: Jin-Geun
authors.initials: JG
authors.lastname: Choi
authors.forename: Kyoung-Rok
authors.initials: KR
authors.lastname: Kim
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D005670:Fusarium / Q000201:enzymology*; D036145:Ginsenosides / Q000138:chemical synthesis* / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D036145:Ginsenosides; C055327:ginsenoside F1; D001617:beta-Glucosidase; C035054:ginsenoside Rg1