title: B7-H1 up-regulated expression in human hepatocellular carcinoma tissue: correlation with tumor interleukin-10 levels.
abstract: OBJECTIVE
  Interleukin-10 (IL-10) plays an important role in hepatocellular carcinoma (HCC) immune evasion. However, the precise mechanism responsible for aberrant IL-10 production remains unknown. B7-H1 expression can predominantly stimulate IL-10 production and contributes to the tumor immune evasion. This study was designed to investigate the expression of B7-H1 and IL-10 in normal liver tissues and HCC samples, and further to evaluate the correlation between B7-H1 expression and IL-10 levels.
  
  
  METHODS
  We detected the B7-H1 and IL-10 expression in sixty HCC tissues and ten healthy liver tissues at mRNA and protein level using RT-PCR and immunohistochemical staining Western-blotting, respectively, and further analyzed the correlation between B7-H1 expression and IL-10 levels in HCC samples.
  
  
  RESULTS
  The results showed that all HCC samples demonstrated the up-regulated expression of B7-H1 and IL-10 at mRNA and protein level compared with normal liver tissues and there was a significant correlation between B7-H1 expression and IL-10 levels (p<0.01).
  
  
  CONCLUSIONS
  These findings for the first time suggest the potential role of B7-H1 in promoting IL-10 production in HCC tissue, and implicate a new mechanism of HCC escape from immune surveillance.
authors.affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Key Laboratory of Combined Multi-organ Transplantation, Ministry of Public Health, Zhejiang Province, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Geng
authors.forename: Junfang
authors.initials: J
authors.lastname: Deng
authors.forename: Guoping
authors.initials: G
authors.lastname: Jiang
authors.forename: Penghong
authors.initials: P
authors.lastname: Song
authors.forename: Zhuoyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D015703:Antigens, CD / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D060890:B7-H1 Antigen; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008113:Liver Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D060890:B7-H1 Antigen; C423236:CD274 protein, human; D012333:RNA, Messenger; D016753:Interleukin-10