title: Quantitative photo activated localization microscopy: unraveling the effects of photoblinking.
abstract: In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
authors.affiliation: Laboratory of Nanoscale Biology, Institute of Bioengineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Paolo
authors.initials: P
authors.lastname: Annibale
authors.forename: Stefano
authors.initials: S
authors.lastname: Vanni
authors.forename: Marco
authors.initials: M
authors.lastname: Scarselli
authors.forename: Ursula
authors.initials: U
authors.lastname: Rothlisberger
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Radenovic
mesh_terms: D003198:Computer Simulation; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008164:Luminescent Proteins / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D018343:Receptors, Adrenergic, beta-2