title: Fiber-optic system for monitoring fast photoactivation dynamics of optical highlighter fluorescent proteins.
abstract: Characterizing the photoactivation performance of optical highlighter fluorescent proteins is crucial to the realization of photoactivation localization microscopy. In contrast to those fluorescence-based approaches that require complex data processing and calibration procedures, here we report a simple and quantitative alternative, which relies on the measurement of small absorption spectra changes over time with a fiber-optic system. Using Dronpa as a representative highlighter protein, we have investigated the capacity of this system in monitoring the fast photoactivation process.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Pei
authors.forename: Lingsong
authors.initials: L
authors.lastname: Qin
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Huang