title: Determination of Oxyclozanide in Beef and Milk using High-Performance Liquid Chromatography System with UV Detector.
abstract: This study was developed and validated for the determination of oxyclozanide residue concentrations in beef and commercial milk, using high-performance liquid chromatography system. Oxyclozanide was successfully separated on a reverse phase column (Xbridge-C(18), 4.6×250 mm, 5 µm) with a mobile phase composed of acetonitrile and 0.1% phosphoric acid (60:40, v/v%). This analytical procedure involved a deproteinization process using acetonitrile for beef and 2% formic acid in acetonitrile for commercial milk, dehydration by adding sodium sulfate to the liquid analytical sample, and a defatting process using n-hexane; after these steps, the extract was exposed to a stream of nitrogen dryness. The final extracted sample was dissolved in the mobile phase and filtered using a 0.45 µm syringe filter. This method had good selectivity and recovery (70.70±7.90-110.79±14.95%) from the matrices. The LOQs ranged from 9.7 to 9.8 µg/kg for beef and commercial milk. The recoveries met the standards set by the CODEX guideline.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Kyul
authors.initials: K
authors.lastname: Jo
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Cho
authors.forename: Hee
authors.initials: H
authors.lastname: Yi
authors.forename: Soo-Min
authors.initials: SM
authors.lastname: Cho
authors.forename: Jin-A
authors.initials: JA
authors.lastname: Park
authors.forename: Chan-Hyeok
authors.initials: CH
authors.lastname: Kwon
authors.forename: Hee-Ra
authors.initials: HR
authors.lastname: Park
authors.forename: Ki-Sung
authors.initials: KS
authors.lastname: Kwon
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin