title: Role of signal transducer and activator of transcription-3 in up-regulation of GFAP after epilepsy.
abstract: Glial Fibrillary Acidic Protein (GFAP) is regarded as a marker of reactive astrogliosis. Recent studies have demonstrated that signal transducer and activator of transcription-3, STAT3 regulates GFAP expression after brain injuries. However, whether STAT3 controls astrogliosis in epilepsy is not clear. In this study, we measured p-STAT3 and GFAP expression during the epileptic process using immunohistochemistry, Western blotting and immunofluorescence. Both p-STAT3 and GFAP expression were highly expressed in the rat hippocampus during different phases of the epileptic process. The augmentation of GFAP expression was inhibited by AG490, a janus kinase 2 (JAK2, an upstream gene of STAT3) inhibitor. The coexpression of p-STAT3 and GFAP was detected in the epileptic rat hippocampus and temporal neocortex of patients. These findings indicate that epilepsy involves the activation of STAT3 that up-regulates the expression of GFAP, which may play an important role in epileptogenesis.
authors.affiliation: Department of Neurology, the Second Affiliated Hospital, Chongqing Medical University, Lin Jiang Road 74, Chongqing 400010, China.
authors.forename: Zucai
authors.initials: Z
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
authors.forename: Zuxia
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xianze
authors.initials: X
authors.lastname: Lei
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.forename: Yunlan
authors.initials: Y
authors.lastname: Xie
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Fang
authors.forename: Yangmei
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D004195:Disease Models, Animal; D004827:Epilepsy / Q000139:chemically induced / Q000503:physiopathology* / Q000601:surgery; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000378:metabolism; D005911:Gliosis / Q000209:etiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010862:Pilocarpine; D051381:Rats; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D010862:Pilocarpine