title: Purification and crystallization of yeast glycosylphosphatidylinositol transamidase subunit PIG-S (PIG-S(71-467)).
abstract: The transfer of glycosylphosphatidylinositol (GPI) anchors onto eukaryotic proteins is catalyzed by the transamidase complex, which is composed of at least five subunits (PIG-K, PIG-S, PIG-T, PIG-U and GPAA1). Here, the recombinant protein PIG-S(71-467) from Saccharomyces cerevisiae, including residues 71-467 of the entire 534-residue protein, was cloned, expressed and purified to homogeneity. The monodisperse protein was crystallized by the vapour-diffusion method. A diffraction data set was collected to 3.2 Å resolution with 91.6% completeness. The crystals belonged to space group C2, with unit-cell parameters a = 106.72, b = 59.33, c = 124.3 Å, β = 114.19°, and contained two molecules in the asymmetric unit.
authors.affiliation: Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. ggrueber@ntu.edu.sg
authors.forename: Neelagandan
authors.initials: N
authors.lastname: Kamariah
authors.forename: Frank
authors.initials: F
authors.lastname: Eisenhaber
authors.forename: Sharmila
authors.initials: S
authors.lastname: Adhikari
authors.forename: Birgit
authors.initials: B
authors.lastname: Eisenhaber
authors.forename: Gerhard
authors.initials: G
authors.lastname: Grüber
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D021122:Protein Subunits; D000217:Acyltransferases; C081007:COOH-terminal signal transamidase