title: eIF5A interacts functionally with eEF2.
abstract: eIF5A is highly conserved from archaea to mammals, essential for cell viability and the only protein known to contain the essential amino acid residue hypusine, generated by a unique posttranslational modification. eIF5A was originally identified as a translation initiation factor due to its ability to stimulate the formation of the first peptide bond. However, recent studies have shown that depletion of eIF5A causes a significant decrease in polysome run-off and an increase in the ribosome transit time, suggesting that eIF5A is actually involved in the elongation step of protein synthesis. We have previously shown that the depletion mutant tif51A-3 (eIF5A(C39Y/G118D)) shows a sicker phenotype when combined with the dominant negative mutant eft2 ( H699K ) of the elongation factor eEF2. In this study, we used the eIF5A(K56A) mutant to further investigate the relationship between eIF5A and eEF2. The eIF5A(K56A) mutant is temperature sensitive and has a defect in protein synthesis, but instead of causing depletion of the eIF5A protein, this mutant has a defect in hypusine modification. Like the mutant tif51A-3, the eIF5A(K56A) mutant is synthetic sick with the mutant eft2 ( H699K ) of eEF2. High-copy eEF2 not only improves cell growth of the eIF5A(K56A) mutant, but also corrects its increased cell size defect. Moreover, eEF2 suppression of the eIF5A(K56A) mutant is correlated with the improvement of total protein synthesis and with the increased resistance to the protein synthesis inhibitor hygromycin B. Finally, the polysome profile defect of the eIF5A(K56A) mutant is largely corrected by high-copy eEF2. Therefore, these results demonstrate that eIF5A is closely related to eEF2 function during translation elongation.
authors.affiliation: Department of Biological Sciences, School of Pharmaceutical Sciences, UNESP - Univ Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Camila A O
authors.initials: CA
authors.lastname: Dias
authors.forename: Ana Paula Borges
authors.initials: AP
authors.lastname: Gregio
authors.forename: Danuza
authors.initials: D
authors.lastname: Rossi
authors.forename: Fábio Carrilho
authors.initials: FC
authors.lastname: Galvão
authors.forename: Tatiana F
authors.initials: TF
authors.lastname: Watanabe
authors.forename: Myung Hee
authors.initials: MH
authors.lastname: Park
authors.forename: Sandro R
authors.initials: SR
authors.lastname: Valentini
authors.forename: Cleslei F
authors.initials: CF
authors.lastname: Zanelli
mesh_terms: D005434:Flow Cytometry; D020652:Peptide Elongation Factor 2 / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D020652:Peptide Elongation Factor 2; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A