title: [Expression of microtubule-associated protein 1 light chain 3 in laryngeal carcinoma].
abstract: OBJECTIVE
  To investigate the expression of microtuble-associated protein 1 light chain 3 (LC3) in laryngeal squamous cell carcinoma (LSCC).
  
  
  METHODS
  The expression of LC3 in 50 cases of LSCC, 45 cases of para-carcinoma, 10 cases of laryngeal papilloma and 16 cases of polyp of vocal cord were detected by immunohistochemistry (MaxVision method). Expression level of LC3 mRNA was assayed by RT-PCR in 41 of LSCC, 41 of para-carcinoma tissue and 11 of polyp of vocal cord.
  
  
  RESULTS
  The positive rates of LC3 protein expression were 60.0%, 93.3%, 90.0%, 93.8% in LSCC tissue, para-carcinoma, laryngeal papilloma and poly of vocal card tissues, respectively. The positive rates of LC3 were significantly lower in LSCC than in para-carcinoma and poly of vocal cord (chi2 = 18.135, P < 0.01). The mRNA levels of LC3 were significantly lower in LSCC than in para-carcinoma and poly of vocal cord (0.57 +/- 0.08 )vs (0.99 +/- 0.11) and (1.07 +/- 0.05) , F = -255.872, P < 0.01. The expression of LC3 were related to tumor location and pathological grade (P < 0.05), but not related to age, T stage, clinical stage and lymphoid metastasis (P > 0.05).
  
  
  CONCLUSIONS
  Expression of LC3 are down-regulated in LSCC. The change of autophagic capacity may play an important role in occurrence and development of LSCC.
authors.affiliation: Department of Otorhinolaryngology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Baoluo
authors.initials: B
authors.lastname: Wan
authors.forename: Junhua
authors.initials: J
authors.lastname: Dong
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Sulin
authors.initials: S
authors.lastname: Zhang
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins