title: Bone regeneration with self-assembling peptide nanofiber scaffolds in tissue engineering for osseointegration of dental implants.
abstract: The aim of this study was to evaluate the correlation between the osseointegration of dental implants and tissue-engineered bone using a nanofiber scaffold, PuraMatrix (PM). The first molar and all premolars in the mandibular regions of dogs were extracted, and three bone defects were prepared with a trephine bur on both sides of the mandible after 4 weeks. The experimental groups were as follows: (1) PM, (2) PM and dog mesenchymal stem cells (dMSCs), (3) PM, dMSCs, and platelet-rich plasma, and (4) control (defect only). Implants were placed in the prepared areas 8 weeks later and were assessed by histologic and histomorphometric analyses (bone-to-implant contact [BIC]). The BICs for groups 1, 2, 3, and 4 were 40.77%, 50.35%, 55.64%, and 30.57%, respectively. The findings indicate that PM may be useful as a scaffold for bone regeneration around dental implants.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Nagoya City University School of Medical Sciences, Nagoya, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kohgo
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yamada
authors.forename: Kenji
authors.initials: K
authors.lastname: Ito
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yajima
authors.forename: Ryoko
authors.initials: R
authors.lastname: Yoshimi
authors.forename: Kazuto
authors.initials: K
authors.lastname: Okabe
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Baba
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018929:Cell Culture Techniques; D003758:Dental Implantation, Endosseous; D015921:Dental Implants; D004285:Dogs; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008334:Mandible / Q000473:pathology / Q000601:surgery; D008336:Mandibular Diseases / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D057139:Nanofibers / Q000737:chemistry*; D016348:Osseointegration / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D010455:Peptides / Q000737:chemistry*; D053657:Platelet-Rich Plasma / Q000502:physiology; D013499:Surface Properties; D013752:Tetracycline; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015921:Dental Implants; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010455:Peptides; C546681:RADA16-I; D013752:Tetracycline; C007740:fluorexon