title: Comparison of the thermal stabilities of the αβ heterodimer and the α subunit of avian myeloblastosis virus reverse transcriptase.
abstract: Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63-kDa α subunit and a 95-kDa β subunit. In this study, we explored the role of the interaction between the α and β subunits on AMV RT stability. The recombinant AMV RT α subunit was expressed in insect cells and purified. It exhibited lower thermal stability than the native AMV RT αβ heterodimer. Unlike the αβ heterodimer, the α subunit was not stabilized by template-primer. These results suggest that interaction between the α and β subunits is important for AMV RT stability.
authors.affiliation: Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Konishi
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nemoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasukawa
authors.forename: Kuniyo
authors.initials: K
authors.lastname: Inouye
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000821:virology; D009189:Avian Myeloblastosis Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016367:Baculoviridae; D001483:Base Sequence; D001717:Birds; D002460:Cell Line; D003001:Cloning, Molecular; D019281:Dimerization; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D010957:Plasmids; D011485:Protein Binding / Q000235:genetics*; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera; D014162:Transfection; D014169:Transformation, Bacterial
chemical_list: D021122:Protein Subunits; D012367:RNA, Viral; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase