title: [Cloning and characterization of 3-hydroxy-3-methylglutary CoA reductase cDNA of Glycyrrhiza uralensis].
abstract: OBJECTIVE
  To clone and analysis the sequence of 3-hydroxy-3-methylglutary CoA reductase (HMGR) cDNA from Glycyrrhiza uralensis.
  
  
  METHODS
  The primers were designed based on the conservative region of HMGR nucleic acids sequence from public database. The target gene was obtained from root of G. uralensis by use of homologous cDNA amplificati on and RACE technologies. The sequence alignment was performed using BLAST. The open reading frame was identified by use of the ORF Finder. The protein domains were defined by use of Prosite software. Clustal was used to conduct multiple amino acid sequence alignment and MEGA 5.0 was used to conduct the phylogenetic tree.
  
  
  RESULTS
  The GuHMGR cDNA sequence was obtained contains 1 842 bp contains a 1 722 bp ORF, encoding 573 amino acids with 3-hydroxy-3-methylglutary CoA reductases family profile. Deduced amino acid sequence had 84% and 76% homology to the amino acid sequence of Pisum sativum, Medicago truncatula.
  
  
  CONCLUSIONS
  The cloning of 3-hydroxy-3-methylglutary CoA reductase (HMGR) cDNA will provide a foundation for exploring the function of HMGR in glycyrrhizin biosynthesis.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. rqx1982@126.com
authors.forename: Qixians
authors.initials: Q
authors.lastname: Rong
authors.forename: Qiaoxian
authors.initials: Q
authors.lastname: Xu
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Liu
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D029882:Glycyrrhiza uralensis / Q000737:chemistry / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D006903:Hydroxymethylglutaryl CoA Reductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D006903:Hydroxymethylglutaryl CoA Reductases