title: Gene expression profiling of mouse aborted uterus induced by lipopolysac charide.
abstract: To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
authors.affiliation: Department of Anatomy, Research Institution of Medical Science, School of Medicine, Chonnam National University, Gwangju, Korea.
authors.forename: Jeong Mi
authors.initials: JM
authors.lastname: Moon
authors.forename: Song Eun
authors.initials: SE
authors.lastname: Lee
authors.forename: Yong Il
authors.initials: YI
authors.lastname: Min
authors.forename: Chaeyong
authors.initials: C
authors.lastname: Jung
authors.forename: Kyu Youn
authors.initials: KY
authors.lastname: Ahn
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Nam