title: [Effects of elicitors on accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root].
abstract: OBJECTIVE
  To observe the effects of a biotic elicitor fungal hyphae extract, an abiotic elicitor methyl jasmonate and their synergistic action on the accumulation of phenolic acids and tanshinones in Salvia miltiorrhiza hairy root.
  
  
  METHODS
  Different elicitors were added to S. miltiorrhiza hairy root, which was subcultured for 21 days, the dry weight and contents of phenolic acids and tanshinones were determined at different harvest-time.
  
  
  RESULTS
  S. miltiorrhiza hairy root growth was significantly inhibited by all three treatments and the accumulation of cryptotanshinone and dihydrotanshinone were promoted by each elicition. As for the accumulation of phenolic acids, there were differences between fungal elicitor and methyl jasmonate treatments, they were promoted by methyl jasmonate while inhibited in a certain extent by fungal hyphae extract.
  
  
  CONCLUSIONS
  Fungal elicitor, methyl jasmonate and their synergistic action have significant influence on accumulation of components in S. miltiorrhiza hairy root, and the effect varies between phenolic acids and tanshinones. There is no correlation between production of water-soluble ingredients and fat-soluble components on the whole under three different treatments.
authors.affiliation: College of Life Sciences, Northwest Agriculture & Forestry University, Yangling 712100, China. shunchang969@126.com
authors.forename: Shuncang
authors.initials: S
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Shuang
authors.initials: S
authors.lastname: Shen
authors.forename: Zongsuo
authors.initials: Z
authors.lastname: Liang
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Yang
mesh_terms: D045784:Abietanes / Q000378:metabolism*; D000085:Acetates / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology*; D062385:Hydroxybenzoates / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000494:pharmacology*; D054883:Oxylipins / Q000494:pharmacology; D010838:Phytophthora / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology; D027561:Salvia miltiorrhiza / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology
chemical_list: D045784:Abietanes; D000085:Acetates; D003517:Cyclopentanes; D005656:Fungal Proteins; D062385:Hydroxybenzoates; D008562:Membrane Glycoproteins; D054883:Oxylipins; D010936:Plant Extracts; C091510:elicitor protein, Phytophthora megasperma; C021751:tanshinone; C072239:methyl jasmonate; C017616:phenolic acid