title: Probing of some compounds as anti-aggregatory additives in the protein refolding process from Escherichia coli inclusion bodies.
abstract: Five compounds of different chemical structure were tested for aggregation suppression during the refolding of porcine and mink growth hormones as model proteins from Escherichia coli inclusion bodies by the dilution method. Of all compounds tested in this work, 3-guanidinopropionic acid (GPA) containing a guanidinium group was the most effective additive for aggregation suppression. Anti-aggregatory properties of GPA were compared with the ones of l-arginine.
authors.affiliation: Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Lithuania.
authors.forename: Albinas
authors.initials: A
authors.lastname: Zilinskas
authors.forename: Jolanta
authors.initials: J
authors.lastname: Sereikaite
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D013006:Growth Hormone / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D006146:Guanidines / Q000737:chemistry*; D002479:Inclusion Bodies / Q000737:chemistry* / Q000378:metabolism; D008907:Mink; D011422:Propionates / Q000737:chemistry*; D058849:Protein Refolding; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification*; D013552:Swine
chemical_list: D006146:Guanidines; D011422:Propionates; D011994:Recombinant Proteins; D013006:Growth Hormone; D001120:Arginine; C005775:guanidinopropionic acid