title: Recombinant modified vaccinia virus ankara (MVA) expressing wild-type human p53 induces specific antitumor CTL expansion.
abstract: The p53 gene product is an attractive target for tumor immunotherapy. The present study aims to understand the potential of MVAp53 vaccine to induce expansion of p53-specific cytotoxic T lymphocyte ex vivo in cancer patients. The result indicated that 14 of 23 cancer patients demonstrated p53-specific IFN-γ production, degranulation, cell proliferation, and lysis of p53 overexpressed human tumor cell lines. These experiments show that MVAp53 stimulation has the potential to induce the expansion of p53-specific cytotoxic T lymphocyte from the memory T cell repertoire. The data suggest that MVAp53 vaccine is an ideal candidate for cancer immunotherapy.
authors.affiliation: Division of Translational Vaccine Research, City of Hope National Medical Center, Duarte, California, USA.
authors.forename: Guang-Yun
authors.initials: GY
authors.lastname: Song
authors.forename: Tumul
authors.initials: T
authors.lastname: Srivastava
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Ishizaki
authors.forename: Simon F
authors.initials: SF
authors.lastname: Lacey
authors.forename: Don J
authors.initials: DJ
authors.lastname: Diamond
authors.forename: Joshua D I
authors.initials: JD
authors.lastname: Ellenhorn
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D019496:Cancer Vaccines / Q000276:immunology*; D015550:Cell Degranulation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D016159:Tumor Suppressor Protein p53 / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D019496:Cancer Vaccines; C521519:MVAp53 vaccine; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D014614:Vaccines, Synthetic; D007371:Interferon-gamma