title: [Intragastric and subcutaneous administration of yolk antibodies against adipocyte plasma membrane proteins on growth and fat deposition in rats].
abstract: OBJECTIVE
  To study the effects of different administrations of antibodies against adipocyte plasma membrane proteins on growth and fat deposition in rats.
  
  
  METHODS
  Ninety six female SD rats that weighed approximately 140 g were allotted randomly into four groups which were given negitive control yolk and positive yolk containing antibody (IgY) against adipocyte plasma membrane (APM) proteins by intragastric administration (i.g.) and subcutaneous injection (s.c.) respectively. Rats were given 1 ml of yolk for every three days in i.g. groups. Rats were given 1 ml of yolk for 4 consecutive days, and the procedure was repeated after one month. The trial lasted for 75 days after which rats were slaughtered for carcass analysis and sampling.
  
  
  RESULTS
  The body weight gain and food intake of rats were not different between treatment and control groups. In i.g. goups, positive yolk decreased mesemteric fat index, paramertrial fat index and perirenal fat index (P < 0.05), reduced serum triglycerides (P < 0.05) and increased serum free fatty acids (FFA) (P < 0.01), and also decreased serum leptin, insulin and TNF-alpha levels ( P < 0.01 or P < 0.05), but did not affect gastrocnemius muscle index and serum cholesterol. In s.c. groups, positive yolk increased gastrocnemius muscle index (P < 0.05), reduced serum triglycerides and serum leptin (P < 0.01), increased serum TNF-alpha (P < 0.05), but did not affect adipose tissue depots, serum FFA, cholesterol and insulin.
  
  
  CONCLUSIONS
  Administration of yolk antibody against APM proteins could effectively improve body composition of rats, and the treatment by intragastric administration could give better effect than by subcutaneous injection.
authors.affiliation: Anhui Agriculture University, Hefei 230036, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Ru-Qian
authors.initials: RQ
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Yong-Qin
authors.initials: YQ
authors.lastname: Chen
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D001823:Body Composition; D004530:Egg Yolk / Q000276:immunology*; D005260:Female; D048788:Growth and Development / Q000502:physiology; D007114:Immunization; D008562:Membrane Glycoproteins / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C439388:APMAP protein, mouse; D000906:Antibodies; D008562:Membrane Glycoproteins