title: Growth suppression effects of recombinant adenovirus expressing human lactoferrin on cervical cancer in vitro and in vivo.
abstract: Human lactoferrin (hLF) is a multifunctional glycoprotein that can inhibit cancer growth. The molecular mechanism of hLF-induced tumor growth inhibition is incompletely understood. Moreover, the adenovirus vector-mediated hLF (Ad-hLF) gene therapy on cervical cancer has not been yet characterized. In this study, the replication-deficient Ad-hLF was used to explore tumor growth suppression effects on cervical cancer in vitro and in vivo. The results showed that the recombinant adenovirus encoding hLF delivery resulted in a more differential tumor growth inhibition, and this growth arrest was caused by cell cycle inhibition at G2/M phase. In addition, Fas, a death-inducing receptor, and Bax, a member of pro-apoptotic Bcl-2 family, were increased in the sample of cervical cancer tissue treated by Ad-hLF. Further, it was also observed that caspase-3 was activated and the expression of anti-apoptotic Bcl-2 was decreased. These results indicated that the growth inhibitory effects of Ad-hLF on cervical cancer were caused by elevated expression of Fas and decreased the ratio of anti- to pro-apoptotic molecule Bcl-2/Bax.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, PR China.
authors.forename: Wen Ye
authors.initials: WY
authors.lastname: Li
authors.forename: Qing Wang
authors.initials: QW
authors.lastname: Li
authors.forename: Zeng Sheng
authors.initials: ZS
authors.lastname: Han
authors.forename: Zhong Liang
authors.initials: ZL
authors.lastname: Jiang
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Xiao Bo
authors.initials: XB
authors.lastname: Zhang
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D007781:Lactoferrin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy*
chemical_list: D007781:Lactoferrin