title: [Regulatory effect of MTA1 on the anoikis of human prostate cancer cells].
abstract: OBJECTIVE
  To study the effects of MTA1 small interfering RNA (siRNA) on the anchorage-independent growth and anoikis of prostate cancer cell line PC-3.
  
  
  METHODS
  After transfection of human prostate cancer PC-3 cells by MTA1 siRNA, we detected the expression of the MTA1 gene by real-time PCR and Western blot, the anchorage-independent growth of the cells by clone formation in soft agar, and their anoikis by DNA fragmentation assay and flow cytometry.
  
  
  RESULTS
  Compared with the control group, MTA1 siRNA transfection significantly decreased the mRNA and protein levels of MTA1, inhibited the anchorage-independent growth of the PC-3 cells, and induced their anoikis, all in a dose- and time-dependent manner (r = 0.935, P = 0.001; r = 0.901, P = 0.0005; r = 0.916, P = 0.0003).
  
  
  CONCLUSIONS
  MTA1 siRNA can inhibit the anchorage-independent growth of prostate cancer cells by inducing their anoikis.
authors.affiliation: Cancer Institute, People's Hospital Affiliated to Jiangsu University, Zhenjiang, Jiangsu 212002, China.
authors.forename: Fei-lun
authors.initials: FL
authors.lastname: Cui
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Gong
authors.forename: Yong-jing
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
mesh_terms: D023102:Anoikis / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006655:Histone Deacetylases / Q000235:genetics*; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D034741:RNA, Small Interfering; D012097:Repressor Proteins / Q000235:genetics*; D015534:Trans-Activators; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C089320:MTA1 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D015534:Trans-Activators; D006655:Histone Deacetylases