title: Highly sensitive and selective detection of thiol-containing biomolecules using DNA-templated silver deposition.
abstract: In this work, we report a new fluorescent method for the label-free assay of thiol-containing amino acids and peptide by use of silver deposited DNA duplex and the intercalating dye. The sensing approach is based on the specific interactions between thiols and DNA-templated silver deposition via robust Ag-S bonds. In the presence of thiols, the intercalating dye gives a dramatic increase in fluorescence as a result of the strong interaction between the intercalator and the released DNA from silver surfaces. The detection limit of this method is lower than or at least comparable to previous fluorescence-based methods, and the turn-on sensing mode offers additional advantage to efficiently reduce background noise. Moreover, the detection and discrimination process can be observed by the naked eye with the aid of an UV transilluminator. This method also exhibits excellent selectivity for these thiol-containing biomolecules over various other amino acids. As far as we know, our method is the first example using the specific interaction between thiols and silver-coated DNA to fabricate a turn-on fluorescent sensor for biological thiols with high sensitivity and selectivity.
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Graduate School of Chinese Academy of Sciences, Chinese Academy of Science, Changchun, Jilin, 130022, China.
authors.forename: Youhui
authors.initials: Y
authors.lastname: Lin
authors.forename: Yu
authors.initials: Y
authors.lastname: Tao
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Fang
authors.initials: F
authors.lastname: Pu
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003545:Cysteine / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D007364:Intercalating Agents; D013438:Sulfhydryl Compounds; D012834:Silver; D004247:DNA; D003545:Cysteine