title: Self-assembly molecularly imprinted polymers of 17β-estradiol on the surface of magnetic nanoparticles for selective separation and detection of estrogenic hormones in feeds.
abstract: This paper reports a surface molecular self-assembly strategy for molecular imprinting on magnetic nanoparticles for selective separation and detection of estrogens in feeds. First, γ-methacryloxypropyltrimethoxysilane (MEMO) was successfully assembled at the surface magnetic nanoparticles through simple free radical polymerization, and subsequently, the copolymerization was further assembled between methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of templates 17β-estradiol (E2). The synthesized magnetic molecularly imprinted polymers for E2 (E2-MMIPs) showed quick separation, large adsorption capacity, high selectivity and fast binding kinetics for E2. Meanwhile, a dispersive solid-phase extraction (DSPE) based on E2-MMIPs has been established for efficient separation and fast enrichment of estrogens from the feeds. The assay exhibited a linear range of 0.1-4 μM for E2 and estriol (E3) with the correlation coefficient above 0.9996 and 0.9994, respectively. Recoveries of E2 from three kinds of feeds spiked at different concentration levels ranged from 92.7% to 97.0% with RSD<4.7%, and recoveries of E3 ranged from 71.9% to 83.1% with RSD<4.9%, respectively. The method is simple and sensitive, and can be used as an alternative tool to effectively separate and enrich the trace of estrogens in agricultural products by HPLC-UV.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Hanzhong Road 140, Nanjing 210029, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Meijuan
authors.initials: M
authors.lastname: Ding
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Jinhui
authors.initials: J
authors.lastname: Xu
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Wen
authors.forename: Wenyu
authors.initials: W
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Kunfang
authors.initials: K
authors.lastname: Ma
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
authors.forename: Shuhu
authors.initials: S
authors.lastname: Du
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D004958:Estradiol / Q000737:chemistry*; D004964:Estriol / Q000032:analysis / Q000302:isolation & purification; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods; D013816:Thermodynamics
chemical_list: D058185:Magnetite Nanoparticles; D004958:Estradiol; D004964:Estriol