title: Comparative study of anti-oncogenic microRNA-145 in canine and human malignant melanoma.
abstract: MicroRNA-145 (miRNA-145; miR-145) is aberrantly expressed in most of human cancers and plays a significant role in carcinogenesis and cancer progression. In the current study, we focused on how miR-145 plays a role in canine and human malignant melanomas. MiR-145 was significantly downregulated in canine malignant melanoma tissues and canine melanoma cell lines, as well as human melanoma cell lines tested. The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested, and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells. At the same time, a suppressive tendency on cell migration in canine melanoma KMeC cells and significant suppression of cell migration in human melanoma A2058 cells by suppressing FASCIN1 were also found. These findings suggest that miR-145 acts as a tumor suppressor in both canine and human malignant melanomas.
authors.affiliation: The United Graduate School of Veterinary Sciences, Gifu University, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Noguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Mori
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Nami
authors.initials: N
authors.lastname: Yamada
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Sasaki
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Akao
authors.forename: Kohji
authors.initials: K
authors.lastname: Maruo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D008545:Melanoma / Q000378:metabolism / Q000662:veterinary*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs