title: Low-dose captopril inhibits wear debris-induced inflammatory osteolysis.
abstract: The role of captopril in titanium alloy particle-induced inflammatory osteolysis was investigated. BALB/c mice (n = 32) were divided into four groups, an untreated control group and three treatment groups given 12.5, 25 or 50 mg/kg per day captopril. Intraperitoneal injections of either 0.9% saline (control) or captopril began 2 days before the introduction of titanium alloy particles and calvaria bone from a syngeneic mouse into established air pouches. Mice were sacrificed 10 days after bone/titanium alloy implantation, and pouch membranes and implants were collected for histological and molecular analysis. Low-dose captopril (12.5 mg/kg per day) was found to inhibit titanium particle-induced tissue inflammation and inflammatory osteolysis. Pouch membrane thickness and inflammatory cellular infiltration were significantly reduced relative to controls. Captopril also inhibited production of the inflammatory cytokines tumour necrosis factor-α, interleukin-1β and receptor activator of nuclear factor-κB ligand compared with controls. This study provides evidence that a low-dose of captopril can inhibit titanium particle-induced inflammatory osteolysis.
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Han
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Peng
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.forename: Xianlong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002216:Captopril / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005260:Female; D007249:Inflammation / Q000150:complications*; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010014:Osteolysis / Q000209:etiology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014025:Titanium
chemical_list: D016207:Cytokines; D017931:DNA Primers; D018836:Inflammation Mediators; D012333:RNA, Messenger; D002216:Captopril; D014025:Titanium