title: [Levels of interlukin-6 and tumor necrosis factor-α in saliva of patients with type 2 diabetes mellitus and oral lichen planus].
abstract: OBJECTIVE
  To study the relationship between type 2 diabetes mellitus (T2DM) and oral lichen planus (OLP) by detecting the level of salivary tumor necrosis factor (TNF-α) and interlukin-6 (IL-6).
  
  
  METHODS
  Subjects were divided into 4 groups: T2DM/OLP group 29 patients, T2DM group 39 patients, OLP group 21 patients, and control group 43 individuals. The salivary interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) were detected by enzyme-linked immuno sorbent assay (ELISA).
  
  
  RESULTS
  (1) The level of salivary IL-6 in patients with T2DM/OLP [(12.30 ± 16.03) ng/L] was significantly higher than those in T2DM [(6.29 ± 5.91) ng/L] and OLP groups [(3.64 ± 4.47) ng/L], P<0.05. The level of salivary IL-6 was significantly lower in OLP group [(3.64 ± 4.47) ng/L] than in control group [(7.91 ± 4.05) ng/L], P<0.001. The level of salivary TNF-α in T2DM group [(8.80 ± 8.41) ng/L] was significantly lower than those in OLP [(14.02 ± 9.65) ng/L] and control groups [(15.02 ± 6.13) ng/L], P<0.05. (2) The level of salivary TNF-α is significantly negative correlated with pH value of saliva in T2DM/OLP group(r=-0.593, P<0.01);The level of salivary TNF-α and IL-6 are significantly positive correlated with waistline in control group(r=0.312,P=0.05).(3) The levels of salivary IL-6 and TNF-α were positively related to OLP clinical type, P<0.05. (4)When OLP played an overlying role on T2DM, the level of TNF-α was weakened and that of IL-6 was strengthened.
  
  
  CONCLUSIONS
  When T2DM and OLP are in concurrence,there is a synergistic effect,and the secretion of IL-6 increases markedly; The level of salivary TNF-α is associated with local oral environment.
authors.affiliation: Department of TCM and Oral Medicine, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian-qiu
authors.initials: JQ
authors.lastname: Jin
authors.forename: Zhen-fang
authors.initials: ZF
authors.lastname: Yuan
authors.forename: Xiao Song
authors.initials: XS
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Guo
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000368:Aged; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis*; D017676:Lichen Planus, Oral / Q000150:complications / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha