title: Low-intensity interval exercise training attenuates coronary vascular dysfunction and preserves Ca²⁺-sensitive K⁺ current in miniature swine with LV hypertrophy.
abstract: Coronary vascular dysfunction has been observed in several models of heart failure (HF). Recent evidence indicates that exercise training is beneficial for patients with HF, but the precise intensity and underlying mechanisms are unknown. Left ventricular (LV) hypertrophy can play a significant role in the development of HF; therefore, the purpose of this study was to assess the effects of low-intensity interval exercise training on coronary vascular function in sedentary (HF) and exercise trained (HF-TR) aortic-banded miniature swine displaying LV hypertrophy. Six months postsurgery, in vivo coronary vascular responses to endothelin-1 (ET-1) and adenosine were measured in the left anterior descending coronary artery. Baseline and maximal coronary vascular conductance were similar between all groups. ET-1-induced reductions in coronary vascular conductance (P < 0.05) were greater in HF vs. sedentary control and HF-TR groups. Pretreatment with the ET type A (ET(A)) receptor blocker BQ-123 prevented ET-1 hypersensitivity in HF animals. Whole cell voltage clamp was used to characterize composite K(+) currents (I(K(+))) in coronary smooth muscle cells. Raising internal Ca(2+) from 200 to 500 nM increased Ca(2+)-sensitive K(+) current in HF-TR and control, but not HF animals. In conclusion, an ET(A)-receptor-mediated hypersensitivity to ET-1, elevated resting LV wall tension, and decreased coronary smooth muscle cell Ca(2+)-sensitive I(K(+)) was found in sedentary animals with LV hypertrophy. Low-intensity interval exercise training preserved normal coronary vascular function and smooth muscle cell Ca(2+)-sensitive I(K(+)), illustrating a potential mechanism underlying coronary vascular dysfunction in a large-animal model of LV hypertrophy. Our results demonstrate the potential clinical impact of exercise on coronary vascular function in HF patients displaying pathological LV hypertrophy.
authors.affiliation: Dept. of Biomedical Science, Univ. of Missouri-Columbia, 1600 E. Rollins, E117 Veterinary Medicine, Columbia, MO 65211, USA. emterc@missouri.edu
authors.forename: Craig A
authors.initials: CA
authors.lastname: Emter
authors.forename: Darla L
authors.initials: DL
authors.lastname: Tharp
authors.forename: Jan R
authors.initials: JR
authors.lastname: Ivey
authors.forename: Venkataseshu K
authors.initials: VK
authors.lastname: Ganjam
authors.forename: Douglas K
authors.initials: DK
authors.lastname: Bowles
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002196:Capillaries / Q000502:physiology; D002316:Cardiotonic Agents / Q000494:pharmacology; D003326:Coronary Circulation / Q000502:physiology; D003327:Coronary Disease / Q000473:pathology / Q000503:physiopathology*; D003331:Coronary Vessels / Q000502:physiology; D004280:Dobutamine / Q000494:pharmacology; D065130:Endothelin A Receptor Antagonists; D019332:Endothelin-1 / Q000378:metabolism; D006339:Heart Rate / Q000502:physiology; D017379:Hypertrophy, Left Ventricular / Q000473:pathology / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D009200:Myocardial Contraction / Q000502:physiology; D010456:Peptides, Cyclic / Q000494:pharmacology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D024681:Potassium Channels, Calcium-Activated / Q000502:physiology*; D044022:Receptor, Endothelin A / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature
chemical_list: D002316:Cardiotonic Agents; D065130:Endothelin A Receptor Antagonists; D019332:Endothelin-1; D010456:Peptides, Cyclic; D024681:Potassium Channels, Calcium-Activated; D044022:Receptor, Endothelin A; D004280:Dobutamine; C072247:cyclo(Trp-Asp-Pro-Val-Leu)