title: Single cell transcriptomics of neighboring hyphae of Aspergillus niger.
abstract: Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
authors.affiliation: Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
authors.forename: Charissa
authors.initials: C
authors.lastname: de Bekker
authors.forename: Oskar
authors.initials: O
authors.lastname: Bruning
authors.forename: Martijs J
authors.initials: MJ
authors.lastname: Jonker
authors.forename: Timo M
authors.initials: TM
authors.lastname: Breit
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
mesh_terms: D001234:Aspergillus niger / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D025301:Hyphae / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D059010:Single-Cell Analysis / Q000379:methods
chemical_list: D012331:RNA, Fungal