title: Ultrasonic-assisted liquid-liquid extraction and HILIC-ELSD analysis of ginsenoside Rb(1), astragaloside IV and dulcitol in sugar-free "Fufangfufangteng Heji".
abstract: A simple method for the extraction and determination of ginsenoside Rb(1), astragaloside IV and dulcitol in sugar-free "Fufangfufangteng Heji" was developed using an ultrasonic-assisted liquid-liquid extraction (UALLE) coupled with Hydrophilic Interaction Liquid Interface Chromatography and Evaporative Light Scattering Detector (HILIC-ELSD) analysis. Good chromatographic separation was achieved using a Phenomenex Luna HILIC column (250mm×4.6mm i.d., 5μm), and a mobile phase consisting of acetonitrile-water at a flow rate of 1.0ml/min with a gradient elution within 25min was also used. Compared to the conventional analysis method, the proposed method had the advantages of a longer column life, shorter analysis time, lower baseline noise, short sample pretreatment time and low consumption of organic solvent. The linear ranges for ginsenoside Rb(1), astragaloside IV and dulcitol were 0.0256-0.179, 0.110-0.770, 0.105-0.630mg/ml, respectively. The recoveries of ginsenoside Rb(1), astragaloside IV and dulcitol during the pharmaceutical preparation were within the range of 97.2-100.3%, and their relative standard deviations were 1.2-3.1%.
authors.affiliation: Guangxi Traditional Chinese Medical University, Nanning 530001, PR China. chinaqjp6380@yahoo.com.cn
authors.forename: Jieping
authors.initials: J
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Li
authors.forename: Kefeng
authors.initials: K
authors.lastname: Mo
authors.forename: Shiying
authors.initials: S
authors.lastname: Lu
mesh_terms: D002138:Calibration; D002316:Cardiotonic Agents / Q000032:analysis / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004338:Drug Combinations; D004376:Galactitol / Q000032:analysis* / Q000737:chemistry; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry; D008027:Light; D059625:Liquid-Liquid Extraction / Q000379:methods; D028321:Plant Preparations / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D013016:Sound; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D002316:Cardiotonic Agents; D004338:Drug Combinations; D036145:Ginsenosides; D028321:Plant Preparations; D012503:Saponins; D014315:Triterpenes; D004376:Galactitol; C052064:astragaloside A; C442759:ginsenoside Rb1