title: Influence of cryoprotectants glycerol and amides, combined with antioxidants on quality of frozen-thawed boar sperm.
abstract: The present study was undertaken to examine whether the cooling and freezing extenders containing a mixture of antioxidants (AOs) catalase, Na-pyruvate and mercaptoethanol and one of three types of cryoprotectants (CPs) would be able to improve the quality of frozen-thawed boar sperm. The collected semen, only the sperm-rich fraction, was diluted 1:1 with Androhep plus™ extender, stored at 15°C for 2 h and centrifuged. The centrifuged sperm pellet was re-suspended in lactose-egg yolk extender and divided into four groups for mixing with freezing extenders containing different kinds of CPs at 5°C: (I) glycerol (GLY) as control; (II) GLY with AOs; (III) dimethyl formamide (DMF) with AOs and (IV) dimethyl acetamide (DMA) with AOs. Processed sperm were packaged in 0.25-mL straws and frozen using a controlled rate freezer. After thawing, the diluted thawed sperm were incubated at 38°C for 10 min and was assessed for motility by CASA, membrane/acrosome integrity by FITC-PNA/PI and DNA integrity (DFI) by SCSA. All sperm parameters evaluated, except DFI, were negatively affected (P<0.001) when using DMF (III) or DMA (IV) as CPs instead of GLY (I and II). Total sperm motility was lower (P<0.001) in the samples supplemented with AOs (32.4 ± 1.2, 23.9 ± 1.5, 6.9 ± 0.7, and 10.3 ± 0.9%, for treatments I, II, III and IV, respectively). The quality of sperm frozen in DMF was not different from DMA (P>0.05). There was no difference in DFI among the studied groups (P>0.05). In conclusion, based on the present results, addition of AOs to cooling and freezing extenders and/or replacement of GLY with DMF or DMA could not improve quality of frozen-thawed boar sperm.
authors.affiliation: Institute of Farm Animal Genetics Mariensee, Federal Research Institute for Animal Health, Neustadt, Germany. ningkakanang@yahoo.com
authors.forename: K
authors.initials: K
authors.lastname: Buranaamnuay
authors.forename: R
authors.initials: R
authors.lastname: Grossfeld
authors.forename: C
authors.initials: C
authors.lastname: Struckmann
authors.forename: D
authors.initials: D
authors.lastname: Rath
mesh_terms: D000177:Acrosome / Q000502:physiology; D000577:Amides; D000818:Animals; D002843:Chromatin / Q000502:physiology; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D005990:Glycerol; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa; D013552:Swine
chemical_list: D000577:Amides; D002843:Chromatin; D003451:Cryoprotective Agents; D005990:Glycerol