title: [Studies on optimizing preparation technics of wumeitougu oral liquid by response surface methodology].
abstract: OBJECTIVE
  To optimize the preparation technics of wumeitougu oral liquid (WTOL) by response surface methodology.
  
  
  METHODS
  Based on the single-factor tests, the times of WTOL extraction, alcohol precipitation concentration and pH value were selected as three factors for box-behnken central composite design. The response surface methodology was used to optimize the parameters of the preparation.
  
  
  RESULTS
  Under the condition of extraction time 1.5 h, extraction times 2.772, the relative density 1.12, alcohol precipitation concentration 68.704%, and pH value 5.0, he theory highest content of Asperosaponin VI was up to 549.908 mg/L. Considering the actual situation, the conditions were amended to three extract times, alcohol precipitation concentration 69%, pH value 5.0, and the content of Dipsacaceae VI saponin examined was 548.63 mg/L which was closed to the theoretical value.
  
  
  CONCLUSIONS
  The optimized preparation technics of WTOL by response surface methodology is reasonable and feasible.
authors.affiliation: Department of Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Key Laboratory of University in Heilongjiang Province, Harbin 150086, China. yxcui0825@163.com
authors.forename: Xiao-cui
authors.initials: XC
authors.lastname: Yu
authors.forename: Gao-feng
authors.initials: GF
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D049917:Dipsacaceae / Q000737:chemistry; D004304:Dosage Forms; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol; D006863:Hydrogen-Ion Concentration; D015233:Models, Statistical; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D027824:Rosaceae / Q000737:chemistry; D012503:Saponins / Q000032:analysis*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014867:Water
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004304:Dosage Forms; D004365:Drugs, Chinese Herbal; D012503:Saponins; C534004:akebia saponin D; D014867:Water; D000431:Ethanol