title: [Study on the effects of hematopoiesis of anemic mice after marrow-suppressed treated by tonifying kidney, invigorating spleen, and removing blood stasis].
abstract: OBJECTIVE
  To observe the effects of tonifying kidney,tonifying spleen,invigorating the circulation of blood on the expression of hematopooietic cytokines of bone marrow suppression induced by chemotherapy.
  
  
  METHODS
  Automated blood analyzer was used to detect the level of RBC and HGB, 14th and 28 days, while real time quantitative RT-PCR was used to detect EPO, EPOR mRNA expression.
  
  
  RESULTS
  Liuwei Dihuang Tang, Buzhong Yiqi Tang and Compound Danshen Decoction group could increase the level of RBC and HGB significantly. Liuwei Dihuang Tang and Buzhong Yiqi Tang groups could increase the mRNA expression level of EPO and EPOR significantly. However, there was no significantly difference when Compound Danshen Decoction group compared with control group on EPO, EPOR mRNA expression level.
  
  
  CONCLUSIONS
  The tonifying kidney, tonifying spleen, invigorate the circulation of blood are stable and reliable as to enhance the role of peripheral blood; tonifying kidney, tonifying spleen can improve EPO, EPOR mRNA expression levels, and promote the proliferation of bone marrow hematopoietic stem cells, and promote the differentiation of erythroid blood cells to increase red blood cell line; And invigorate the circulation of blood promote hematopoietic mechanisms have to be further studied.
authors.affiliation: The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China. ayuan1107@163.com
authors.forename: Han-yuan
authors.initials: HY
authors.lastname: Dai
authors.forename: Ke-jian
authors.initials: KJ
authors.lastname: Cao
authors.forename: An-bin
authors.initials: AB
authors.lastname: Zhao
authors.forename: Xiao-yin
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000740:Anemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects; D001853:Bone Marrow / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003520:Cyclophosphamide / Q000008:administration & dosage; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004921:Erythropoietin / Q000235:genetics / Q000378:metabolism; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008815:Mice, Inbred Strains; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D017467:Receptors, Erythropoietin / Q000235:genetics / Q000378:metabolism; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction; D012333:RNA, Messenger; D017467:Receptors, Erythropoietin; D004921:Erythropoietin; D003520:Cyclophosphamide