title: Down-regulation of TWIST decreases migration and invasion of laryngeal carcinoma Hep-2 cells by regulating the E-cadherin, N-cadherin expression.
abstract: OBJECTIVE
  The transcription factor TWIST is an important factor in regulation of the epithelial-mesenchymal transition (EMT), which represents the primary stages during the metastasis of tumors. To identify the role of TWIST in the regulation of metastasis in laryngeal carcinoma Hep-2 cells, we investigated whether the alteration of TWIST has an effect on the Hep-2 cells morphology and whether the alteration of TWIST has an effect on the expression of E-cadherin, N-cadherin as well as the ability of cell motion, migration, and invasion.
  
  
  METHODS
  Morphological changes of Hep-2 cells that were transfected a mircoRNA against TWIST vector were observed by the reserved microscope. Reverse transcription-polymerase chain reaction was performed in order to examine the mRNA expression of TWIST, E-cadherin, and N-cadherin. Western blotting was performed to examine the protein expression of TWIST, E-cadherin, and N-cadherin. Cell motion ability was examined by Scratch-wound assay. Transwell(™) chamber assays were used to determine cell migration and invasion.
  
  
  RESULTS
  Transfecting a mircoRNA down-regulated TWIST expression at mRNA and protein levels. Down-regulation of TWIST expression induced morphological changes, such as the inversion of the EMT. Moreover, down-regulation of TWIST expression up-regulated E-cadherin and down-regulated N-cadherin expressions at mRNA and protein levels, respectively. Furthermore, we confirmed that down-regulation of TWIST expression decreased the motion, invasion, and migration ability of the Hep-2 cells.
  
  
  CONCLUSIONS
  Down-regulation of TWIST expression decreases migration and invasion of laryngeal carcinoma Hep-2 cells by regulation of the E-cadherin, N-cadherin expression.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, 250021, Peoples' Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Hui-zheng
authors.initials: HZ
authors.lastname: Li
authors.forename: Su-mei
authors.initials: SM
authors.lastname: Lu
authors.forename: Jia-jun
authors.initials: JJ
authors.lastname: Tian
authors.forename: Ju-ke
authors.initials: JK
authors.lastname: Ma
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D001483:Base Sequence; D015820:Cadherins / Q000032:analysis*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D051879:Twist-Related Protein 1 / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D015703:Antigens, CD; C050361:CDH2 protein, human; D015820:Cadherins; D051879:Twist-Related Protein 1