title: Uncoupling chitosanase production from chitosan.
abstract: There is a growing interest in chitosanases as enzymatic tools to hydrolyze chitosan into bioactive forms: low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS). However chitosanases are still expensive and methods of large-scale production of these enzymes are not yet established. The article reviews the approaches used for chitosanase production in various bacterial hosts, pointing out the difficulties resulting from the necessity to include chitosan into the medium composition. A mutated Streptomyces host allows for the efficient production of several chitosanases originating from actinobacteria in the absence of chitosan as inducer.
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, (Québec) Canada. Ryszard.Brezezinski@USherbrooke.ca
authors.forename: Ryszard
authors.initials: R
authors.lastname: Brzezinski
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D048271:Chitosan / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D009844:Oligosaccharides / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D048271:Chitosan; D006026:Glycoside Hydrolases; C019868:chitosanase