title: [Extraction of agglutinin from Oncomelania hupensis and its haemagglutination activity].
abstract: OBJECTIVE
  To explore the extraction methods of agglutinin from Oncomelania hupensis snail and study its haemagglutination activity.
  
  
  METHODS
  Protein obtained by ammonium sulfate fractionation precipitation with 20%-100% saturation of ammonium sulfate. Its haemagglutination activity was determined by rabbit erythrocytes. The precipitation which could agglutinate rabbit erythrocytes was diluted with 2.5 mg/ml D-galactose, D-fructose, D-glucose, saccharose, maltose and lactose, respectively, and then their haemagglutination activity was tested. Snail agglutinin were incubated at different temperatures (25-90 degrees C) and assayed for agglutinating activity. The effect of pH on the haemagglutination activity was determined by using the PBS buffer at different pH values (3.0-10.0).
  
  
  RESULTS
  Oncomelania snail agglutinin exhibited high haemagglutination activity in 20%-40% saturated ammonium sulfate pellet. Lactose and galactose could inhibit the haemagglutination activity of snail agglutinin. The agglutinin showed maximum activity at pH 7.0. In temperature range of 30-70 degrees C, the haemagglutination activity decreased with increasing temperature, and all activity lost beyond 80 degrees C.
  
  
  CONCLUSIONS
  Galactose/lactose specific agglutinin exists in Oncomelania snail, its haemagglutination activity is affected by pH and temperature.
authors.affiliation: Wannan Medical College, Wuha 241000, China.
authors.forename: Huai-ming
authors.initials: HM
authors.lastname: Peng
authors.forename: Chao-pin
authors.initials: CP
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Jin-song
authors.initials: JS
authors.lastname: Zhao
authors.forename: Shu-lin
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D000373:Agglutinins / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D004903:Erythrocyte Aggregation; D004912:Erythrocytes / Q000187:drug effects; D006384:Hemagglutination; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D012908:Snails / Q000737:chemistry*; D013696:Temperature
chemical_list: D000373:Agglutinins