title: Core/shell nanoparticles for multiple biological detection with enhanced sensitivity and kinetics.
abstract: The paper shows the different methods to attach a molecule to detect streptavidin to a dielectric particle made of a rare-earth oxide core and a polysiloxane shell containing fluorescein. First, the detection of streptavidin binding on a biotinylated gold substrate can be achieved in three ways: the shift of the surface plasmon resonance of the substrate and the double luminescence (organic and inorganic) of the core/shell particle. Second, these detections are efficient even after elimination upon thermal annealing of all the undesired molecules that skew the assays. Finally, the particle that ballasts the protein enhances its binding kinetics and increases the localized surface plasmon resonance shift that detects the binding.
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS UMR 5620, Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), F-69622 Villeurbanne Cedex, France.
authors.forename: Anne-Charlotte
authors.initials: AC
authors.lastname: Faure
authors.forename: Grégory
authors.initials: G
authors.lastname: Barbillon
authors.forename: Meigui
authors.initials: M
authors.lastname: Ou
authors.forename: Gilles
authors.initials: G
authors.lastname: Ledoux
authors.forename: Olivier
authors.initials: O
authors.lastname: Tillement
authors.forename: Stéphane
authors.initials: S
authors.lastname: Roux
authors.forename: Damien
authors.initials: D
authors.lastname: Fabregue
authors.forename: Armel
authors.initials: A
authors.lastname: Descamps
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Bijeon
authors.forename: Christophe A
authors.initials: CA
authors.lastname: Marquette
authors.forename: Claire
authors.initials: C
authors.lastname: Billotey
authors.forename: Cécile
authors.initials: C
authors.lastname: Jamois
authors.forename: Taha
authors.initials: T
authors.lastname: Benyatou
authors.forename: Pascal
authors.initials: P
authors.lastname: Perriat