title: Oxidative stress and histological alterations produced by dietary copper in the fresh water bivalve Diplodon chilensis.
abstract: The aim of this work was to study the oxidative stress effects and histological alterations caused by dietary copper on the filter-feeding freshwater mussel Diplodon chilensis. Bivalves were fed during 6 weeks with the green algae Scenedesmus vacuolatus previously exposed to copper. Metal concentration in algae cultures and bivalve digestive gland was measured by TXRF. A maximum accumulation of 0.49 μg Cu/mg protein was detected at week 6. Also at this week, the hepatosomatic index (HSI) showed the highest decrease (50%) in response to Cu exposure. SOD and GST activities were significantly increased at weeks 4, 5 and 6, reaching an activity on average 50% higher than in controls for GST. CAT activity and GSH increased significantly at weeks 5 and 6. Despite this response, oxidative damage measured as TBARS and carbonyl groups contents increased significantly at weeks 4, 5 and 6, respectively. Digestive tubule and duct atrophy and cell-type replacement in treated mussels were observed by histological studies. The presence of intracellular rhodanine-positive granules, suggests copper accumulation in intracellular vacuoles of digestive cells.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428, Buenos Aires, Argentina. sabatini@bg.fcen.uba.ar
authors.forename: Sebastián E
authors.initials: SE
authors.lastname: Sabatini
authors.forename: Iara
authors.initials: I
authors.lastname: Rocchetta
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Nahabedian
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Luquet
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Eppis
authors.forename: Laura
authors.initials: L
authors.lastname: Bianchi
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Ríos de Molina
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000033:anatomy & histology* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D005618:Fresh Water; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D044444:Scenedesmus; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione