title: JNK1, but not JNK2, is required in two mechanistically distinct models of inflammatory arthritis.
abstract: The roles of the c-Jun N-terminal kinases (JNKs) in inflammatory arthritis have been investigated; however, the roles of each isotype (ie, JNK1 and JNK2) in rheumatoid arthritis and conclusions about whether inhibition of one or both is necessary for amelioration of disease are unclear. By using JNK1- or JNK2-deficient mice in the collagen-induced arthritis and the KRN T-cell receptor transgenic mouse on C57BL/6 nonobese diabetic (K/BxN) serum transfer arthritis models, we demonstrate that JNK1 deficiency results in protection from arthritis, as judged by clinical score and histological evaluation in both models of inflammatory arthritis. In contrast, abrogation of JNK2 exacerbates disease. In collagen-induced arthritis, the distinct roles of the JNK isotypes can, at least in part, be explained by altered regulation of CD86 expression in JNK1- or JNK2-deficient macrophages in response to microbial products, thereby affecting T-cell-mediated immunity. The protection from K/BxN serum-induced arthritis in Jnk1(-/-) mice can also be explained by inept macrophage function because adoptive transfer of wild-type macrophages to Jnk1(-/-) mice restored disease susceptibility. Thus, our results provide a possible explanation for the modest therapeutic effects of broad JNK inhibitors and suggest that future therapies should selectively target the JNK1 isoform.
authors.affiliation: Department of Pharmacology and Pharmacotherapy, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Katja
authors.initials: K
authors.lastname: Denninger
authors.forename: Susanne
authors.initials: S
authors.lastname: Rasmussen
authors.forename: Jeppe Madura
authors.initials: JM
authors.lastname: Larsen
authors.forename: Catrine
authors.initials: C
authors.lastname: Orskov
authors.forename: Steen
authors.initials: S
authors.lastname: Seier Poulsen
authors.forename: Poul
authors.initials: P
authors.lastname: Sørensen
authors.forename: Jan Pravsgaard
authors.initials: JP
authors.lastname: Christensen
authors.forename: Harald
authors.initials: H
authors.lastname: Illges
authors.forename: Niels
authors.initials: N
authors.lastname: Odum
authors.forename: Tord
authors.initials: T
authors.lastname: Labuda
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000150:complications / Q000201:enzymology* / Q000276:immunology / Q000473:pathology*; D051940:B7-2 Antigen / Q000378:metabolism; D024043:Collagen Type II / Q000276:immunology; D004195:Disease Models, Animal; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D007249:Inflammation / Q000150:complications / Q000201:enzymology* / Q000473:pathology*; D007596:Joints / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000201:enzymology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D048055:Mitogen-Activated Protein Kinase 8 / Q000172:deficiency / Q000378:metabolism*; D048056:Mitogen-Activated Protein Kinase 9 / Q000172:deficiency / Q000378:metabolism; D044967:Serum; D015854:Up-Regulation
chemical_list: D051940:B7-2 Antigen; D024043:Collagen Type II; D048056:Mitogen-Activated Protein Kinase 9; D048055:Mitogen-Activated Protein Kinase 8