title: Expression and mutation analysis of heterogeneous nuclear ribonucleoprotein G in human oral cancer.
abstract: We previously reported that wild type (wt) hnRNP G exhibited tumor suppressive activity in human oral squamous cell carcinoma (HOSCC) cell lines lacking hnRNP G. Wt hnRNP G markedly inhibited the proliferation capacity, anchorage independency and in vivo tumorigenicity of HOSCC cells and notably enhanced the DNA repair capabilities of these cells. In the present study, we studied the genetic and expression states of hnRNP G in normal, premalignant and malignant human oral tissues to further understand the relationship between the hnRNP G alterations and the development of human oral cancer. To correlate the cancer development and the level of hnRNP G expression, we performed an immunohistochemistry staining of hnRNP G in normal, premalignant and malignant human oral tissues. Moreover, we examined the entire coding regions of hnRNP G from selected samples to understand the cause of the alterations of the gene expression. The expression of hnRNP G was notably decreased or completely abolished in 80% of premalignant-dysplastic and malignant oral epithelial tissues, whereas 100% of normal and 90% of hyperplastic non-dysplastic epithelium showed high level of hnRNP G in the nucleus of the basal cell layers. Approximately 80% of HOSCC lacking the expression of hnRNP G showed genetic alteration in hnRNP G, i.e., point mutation and exonic deletion. This study suggest that genetic alterations and aberrant expression of hnRNP G occurring during oral carcinogenesis might be useful markers for the early detection of human oral cancer.
authors.affiliation: School of Dentistry, University of California, Los Angeles, CA, USA. kshin@dentistry.ucla.edu
authors.forename: Ki-Hyuk
authors.initials: KH
authors.lastname: Shin
authors.forename: Reuben H
authors.initials: RH
authors.lastname: Kim
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Mo K
authors.initials: MK
authors.lastname: Kang
authors.forename: David
authors.initials: D
authors.lastname: Elashoff
authors.forename: Russell
authors.initials: R
authors.lastname: Christensen
authors.forename: Ana
authors.initials: A
authors.lastname: Pucar
authors.forename: No-Hee
authors.initials: NH
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism
chemical_list: D034441:Heterogeneous-Nuclear Ribonucleoproteins; C510272:RBMX protein, human