title: [Selection and genotyping of lactobacillus with potential preventive effect by repetitive element sequence-based PCR analysis].
abstract: We selected and characterized isolates of Lactobacillus crispatus (L. crispatus) for potential preventing infections of the female reproductive tract. We cultured vaginal swabs from healthy volunteers on de Man, Rogosa and Sharpe (MRS) agar and identified the isolates at the species level by 16S rRNA sequence and genotyped the isolates of Lactobacillus by PCR amplification of repetitive bacterial DNA elements (rep-PCR). Furthermore, 10 L. crispatus strains were assessed for hydrogen peroxide (H2O2) and acid production. Overall 65 isolates were confirmed to be Lactobacillus by sequence analogy, among them 19 were L. crispatus, 17 were Lactobacillus jensenii and 12 were Lactobacillus fermentum. rep-PCR produced specie and strain-specific genomic fingerprints for the Lactobacillus isolates. The selected 10 L. crispatus isolates produced highly acidic environment after growth in MRS. The isolates T22-3 and T29-5 demonstrated high production of H2O2. This study indicated that there are individual differences with vaginal Lactobacillus colonization, and strain diversity within vaginal L. crispatus isolates, T22-3 and T29-5 might be candidates for restoring urogenital health environment in females.
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Su
authors.forename: Chunhong
authors.initials: C
authors.lastname: Hu
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
mesh_terms: D000328:Adult; D005260:Female; D005838:Genotype; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D020071:Interspersed Repetitive Sequences; D007778:Lactobacillus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014621:Vagina / Q000382:microbiology*
chemical_list: D006861:Hydrogen Peroxide