title: Recombinant Wolbachia heat shock protein 60 (HSP60) mediated immune responses in patients with lymphatic filariasis.
abstract: Wolbachia, an endosymbiont present in filarial nematodes, have been implicated in a variety of roles, including the worm development and survival. Elucidation of the role of Wolbachia in filarial nematode biology and pathogenesis has become the focus of many studies and its contribution to parasite survival or immune response is still unclear. Recombinant Wolbachia HSP60 decreases T cell activation and lymphoproliferation in filarial infected people compared to endemic controls as observed by the assessment of T cell activation markers and cytokine responses in the peripheral blood mononuclear cells. Reduced T cell activation may be linked to T regulatory cell activity since it is associated with increased expression of CTLA4 and CD25 on CD4(+) T cells in filarial infected group upon stimulation with recombinant Wolbachia HSP60. In addition, elevated interleukin-10 and TGF-β cytokines corroborate the reduced CD4(+) T cell activation and interferon-γ observed upon recombinant Wolbachia HSP60 stimulation in filarial patients. Hence, these findings indicate that Wolbachia HSP60 may also contribute to the immune modulation seen in filarial patients.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600025, India.
authors.forename: Chandanapurath
authors.initials: C
authors.lastname: Shiny
authors.forename: Nagampalli S A
authors.initials: NS
authors.lastname: Krushna
authors.forename: Subash
authors.initials: S
authors.lastname: Babu
authors.forename: S
authors.initials: S
authors.lastname: Elango
authors.forename: Guruswamy
authors.initials: G
authors.lastname: Manokaran
authors.forename: Rangarajan Badri
authors.initials: RB
authors.lastname: Narayanan
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D017178:Brugia malayi / Q000276:immunology / Q000382:microbiology*; D060908:CTLA-4 Antigen / Q000276:immunology; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D002648:Child; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology; D005260:Female; D006801:Humans; D056747:Immunomodulation / Q000187:drug effects; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D013559:Symbiosis; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000276:immunology; D020577:Wolbachia / Q000737:chemistry / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; D018834:Chaperonin 60; C508017:IL2RA protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma