title: Strain-promoted cycloadditions of cyclic nitrones with cyclooctynes for labeling human cancer cells.
abstract: Strain-promoted cycloadditions of cyclic nitrones with cyclooctynes proceed with rate constants up to 3.38 ± 0.31 M(-1) s(-1) in CD(3)CN, or 59 times faster than the analogous reaction of azides. This highly specific modular labeling strategy can be applied for direct labeling of proteins and for live cell imaging of cancer cells.
authors.affiliation: Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, Canada.
authors.forename: Craig S
authors.initials: CS
authors.lastname: McKay
authors.forename: Jessie A
authors.initials: JA
authors.lastname: Blake
authors.forename: Jenny
authors.initials: J
authors.lastname: Cheng
authors.forename: Dana C
authors.initials: DC
authors.lastname: Danielson
authors.forename: John Paul
authors.initials: JP
authors.lastname: Pezacki
mesh_terms: D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D045744:Cell Line, Tumor; D003500:Cyclization; D066246:ErbB Receptors / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D009589:Nitrogen Oxides / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D009589:Nitrogen Oxides; C015388:nitrones; D066246:ErbB Receptors