title: 2-Oxabutane as a substitute for internal monomer units of oligosaccharides to create lectin ligands.
abstract: The synthesis of bioactive oligosaccharides is too tedious to scale up for commercialization. However, structurally simplified glycomimetics are commercializable, if they can be synthesized much more easily than the oligosaccharides while having a comparable bioactivity. In this study, we propose a 2-oxabutane (OB) structure as an imitation of the internal monosaccharide units in oligosaccharides. Two trimannoside and three pentamannoside OB-glycomimics were synthesized in remarkably short steps. Among them, Manα1-OB-2Man 10, a trimannoside mimic, showed eight-fold affinity toward concanavalin A (ConA) relative to methyl mannoside in latex agglutination lectin assay and equilibrium dialysis assay (EDA), while the other mimics showed three- to four-fold affinities. EDA indicated that the bindings between each mimic molecule and a ConA subsite were all in one-to-one stoichiometry and thus these mimics were monovalent ligands, excluding multivalence effect for the high affinities. The strong affinity of 10 could be explained by the occupation of two mannose binding sites of a ConA subsite by its two mannose units. Mimic 10 proved to be even a better ligand for ConA than the natural disaccharide Manα1,2Man, while been much more easy to synthesize, thereby illustrating the potential of the approach here presented.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J2-10, 4259 Nagatsutacho, Midoriku, Yokohama 226-8501, Japan.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Yang
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kawada
authors.forename: Lina
authors.initials: L
authors.lastname: Bai
authors.forename: Daijiro
authors.initials: D
authors.lastname: Kubota
authors.forename: Hideya
authors.initials: H
authors.lastname: Yuasa
mesh_terms: D003208:Concanavalin A / Q000737:chemistry*; D005026:Ethylene Glycols / Q000737:chemistry*; D008024:Ligands; D008738:Methyl Ethers / Q000737:chemistry*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D005026:Ethylene Glycols; D008024:Ligands; D008738:Methyl Ethers; D009844:Oligosaccharides; D003208:Concanavalin A