title: The use of oligoperoxide-coated magnetic nanoparticles to label stem cells.
abstract: Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated gamma-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated gamma-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers the possibility of additional modification by grafting compounds that reduce non-specific protein adsorption.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Daniela
authors.initials: D
authors.lastname: Sponarová
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Miroslava
authors.initials: M
authors.lastname: Trchová
authors.forename: Pavla
authors.initials: P
authors.lastname: Jendelová
authors.forename: Vit
authors.initials: V
authors.lastname: Herynek
authors.forename: Nataliya
authors.initials: N
authors.lastname: Mitina
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaichenko
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Stoika
authors.forename: Petr
authors.initials: P
authors.lastname: Lesný
authors.forename: Eva
authors.initials: E
authors.lastname: Syková
mesh_terms: D000818:Animals; D002470:Cell Survival; D005269:Femur / Q000166:cytology; D005290:Ferric Compounds / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D059630:Mesenchymal Stem Cells / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D013499:Surface Properties
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide