title: Preliminary study for simultaneous detection and quantification of androgenic anabolic steroids using ELISA and pattern recognition techniques.
abstract: A first step towards the multidetection, identification and quantification of anabolic androgenic steroids by enzyme-linked immunosorbent assay (ELISA) has been performed in this study. This proposal combines multiple competitive ELISA assays with different cross-reactivity profiles and multivariate data analysis techniques. Data have been analyzed by principal component analysis in conjunction with a novel K-nearest line classifier. This proposal allows simultaneous detection of up to four different steroids in the range of concentration from 0.1 to 316.2 nM with a total rate of 90.6% of correct detection, even in the presence of cross-reactivities. A methodology for concentration prediction is also presented with satisfactory results.
authors.affiliation: Intelligent Signal Processing Group, Department of Electronics, University of Barcelona, Martí i Franques 1, 08028 Barcelona, Spain. dcalvo@el.ub.es
authors.forename: Daniel
authors.initials: D
authors.lastname: Calvo
authors.forename: Núria
authors.initials: N
authors.lastname: Tort
authors.forename: J Pablo
authors.initials: JP
authors.lastname: Salvador
authors.forename: M-Pilar
authors.initials: MP
authors.lastname: Marco
authors.forename: Fabiana
authors.initials: F
authors.lastname: Centi
authors.forename: Santiago
authors.initials: S
authors.lastname: Marco
mesh_terms: D000728:Androgens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D010363:Pattern Recognition, Automated / Q000379:methods*; D013256:Steroids / Q000032:analysis*
chemical_list: D000728:Androgens; D013256:Steroids