title: Purification, crystallization and preliminary crystallographic analysis of recombinant Lac15 from a marine microbial metagenome.
abstract: Laccases are members of the blue multi-copper oxidase family that can oxidize a wide range of aromatic compounds. A new bacterial laccase (Lac15) has recently been obtained from a marine microbial metagenome from the South China Sea and characterized. In this work, recombinant Lac15 was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.2 Å resolution. The crystal belonged to space group C121, with unit-cell parameters a = 123.41, b = 91.36, c = 86.157 Å, β = 112.10°.
authors.affiliation: Modern Experiment Technology Center and School of Life Sciences, Anhui University, Hefei, People's Republic of China.
authors.forename: Honghua
authors.initials: H
authors.lastname: Ge
authors.forename: Peisong
authors.initials: P
authors.lastname: Xu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Zemin
authors.initials: Z
authors.lastname: Fang
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D042845:Laccase / Q000737:chemistry* / Q000302:isolation & purification; D054892:Metagenome; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D042845:Laccase