title: Measuring intracellular hsp70 in leukocytes by flow cytometry.
abstract: Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including heat shock, oxidative stress, free radicals, UV radiation, and heavy metals. This unit details a method, with supporting protocols, for the measurement of their expression in peripheral blood leukocytes by flow cytometry.
authors.affiliation: Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, Armidale, Australia.
authors.forename: Linda L
authors.initials: LL
authors.lastname: Agnew
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D013048:Specimen Handling
chemical_list: D018840:HSP70 Heat-Shock Proteins