title: [Antitumor effect by combination of shRNA interfering plasmid targeting PKM2 with recombinant endostatin].
abstract: OBJECTIVE
  To investigate the enhancement effect of the combination of shRNA interfering plasmid targeting PKM2 with recombinant Endostatin in the treatment of lung cancer.
  
  
  METHODS
  Twenty five BABL/nu/nu mice bearing A549 lung cancer were divided into 5 groups (NS control, psh-Control, psh-PKM2 treated group, Endostar treated group, psh-PKM2+Endostar treated group) and treated with shRNA interfering plasmid targeting PKM2 and recombinant Endostatin respectively or in combination. The expression of PKM2 in A549 detected with immunofluorescent assay. The interference effect of psh-PKM2 was determined by Western blot. The tumor volume, microvessel density (MVD), apoptosis index (AI) and side effects were observed.
  
  
  RESULTS
  The combination treatment of RNA interfering plasmid targeting PKM2 with recombinant Endostatin inhibited tumor growth obviously (P < 0.05); The combination group revealed a decreased MVD and an increased AI (P < 0.05).
  
  
  CONCLUSIONS
  The combination of shRNA interfering plasmid targeting PKM2 with recombinant Endostatin might enhance anti-tumor effect by increasing the apoptosis of the cancer cell.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jing-Zhi
authors.initials: JZ
authors.lastname: Mao
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Guo
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Shi
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Zhao
authors.forename: Han-Shuo
authors.initials: HS
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D043169:Endostatins / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010957:Plasmids / Q000235:genetics; D011770:Pyruvate Kinase / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D011994:Recombinant Proteins / Q000627:therapeutic use
chemical_list: D043169:Endostatins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D011770:Pyruvate Kinase