title: Anti-biofouling properties of an amphoteric polymer brush constructed on a glass substrate.
abstract: An amphoteric copolymer brush of methacrylic acid (MA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) was prepared by reversible addition-fragmentation chain-transfer (RAFT) polymerization using both a free chain transfer agent (n-butylsulfanylthiocarbonylsulfanyl-2-methyl propionic acid) and a radical initiator (4,4'-azobis(4-cyanopentanoic acid)) covalently fixed to a glass substrate. An aqueous solution of the copolymer, Poly(MA-r-DMAEMA), which was simultaneously obtained in liquid phase, had a sufficiently small polydispersity in its molecular weight. The copolymer brush showed effective suppression of non-specific adsorption of bovine serum albumin and egg white lysozyme to the brush. In contrast, both negatively charged PolyMA and positively charged PolyDMAEMA brushes significantly adsorbed the proteins irrespective of their net charges. Upon ion beam irradiation, furthermore, a hollow space with a designed shape could be made on the glass substrate, and both HEK293 and HepG2 cells non-specifically adhered to the space, forming aggregates, while no adhesion to the non-treated area on the brush was observed. These results suggest that the amphoteric polymer brushes will be useful materials for biomedical applications.
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, University of Toyama, Toyama, Japan. kitano@eng.u-toyama.ac.jp
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kitano
authors.forename: Takuya
authors.initials: T
authors.lastname: Kondo
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kamada
authors.forename: Shintaroh
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Makoto
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kohji
authors.initials: K
authors.lastname: Ohno
mesh_terms: D057219:Biofouling / Q000517:prevention & control*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D005898:Glass / Q000737:chemistry; D056945:Hep G2 Cells; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; C049840:2-(dimethylamino)ethyl methacrylate