title: Characterization of biocompatible NiCo2O4 nanoparticles for applications in hyperthermia and drug delivery.
abstract: Monodispersed, superparamagnetic nickel cobaltite (NCO) nanoparticles were functionalized using mercaptopropionic acid (MPA). MPA conjugates with NCO forming a metal-carboxylate linkage, with the MPA-MPA interaction occurring via formation of disulfide bonds, leaving another carboxyl end free for additional conjugation. The cytotoxicity studies on NCO-MPA show cell viability of ∼100% up to a dosage of 40 μg/mL on SiHa, MCF7, and B16F10 cell lines, and on mouse primary fibroblasts. Time-dependent cell viability studies done for a duration of 72 hours showed the cell lines' viability up to 80% for dosages as high as 80 μg/mL. Negligible leaching (<5 ppm) of ionic Co or Ni was noted into the delivery medium. Upon subjecting the NCO-MPA dispersion (0.1 mg/mL) to radiofrequency absorption, the nanoparticles were heated to 75°C within 2 minutes, suggesting its promise as a magnetic hyperthermia agent. Furthermore, the amino acid lysine and the drug cephalexin were successfully adducted to the NCO system, suggesting its potential for drug delivery.
  
  
  BACKGROUND
  NCO-MPA nanopartciles were found to be promising magnetic hyperthermia agents, suggesting potential future clinical applications.
authors.affiliation: Department of Electronic Science, Fergusson College, Pune, India. sangeetakale2004@gmail.com
authors.forename: Sangeeta N
authors.initials: SN
authors.lastname: Kale
authors.forename: Anil D
authors.initials: AD
authors.lastname: Jadhav
authors.forename: Seema
authors.initials: S
authors.lastname: Verma
authors.forename: Soumya J
authors.initials: SJ
authors.lastname: Koppikar
authors.forename: Ruchika
authors.initials: R
authors.lastname: Kaul-Ghanekar
authors.forename: Sanjay D
authors.initials: SD
authors.lastname: Dhole
authors.forename: Satishchandra B
authors.initials: SB
authors.lastname: Ogale
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002506:Cephalexin / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D005334:Fever / Q000188:drug therapy*; D006801:Humans; D008239:Lysine / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D053758:Nanoparticles
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D008239:Lysine; D002506:Cephalexin