title: Burkholderia zhejiangensis sp. nov., a methyl-parathion-degrading bacterium isolated from a wastewater-treatment system.
abstract: The taxonomic status of a methyl-parathion-degrading strain, OP-1(T), isolated from a wastewater-treatment system in China, was determined using a polyphasic approach. The rod-shaped cells were Gram-staining-negative, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain belonged to the genus Burkholderia, as it appeared closely related to Burkholderia glathei ATCC 29195(T) (97.4 % sequence similarity), Burkholderia sordidicola KCTC 12081(T) (96.5 %) and Burkholderia bryophila LMG 23644(T) (96.3 %). The major cellular fatty acids, C(16:0), C(17:0) cyclo and C(18:1)ω7c, were also similar to those found in established members of the genus Burkholderia. The genomic DNA G+C content of strain OP-1(T) was 59.4 mol%. The level of DNA-DNA relatedness between the novel strain and the closest recognized species, Burkholderia glathei ATCC 29195(T), was only 30 %. Based on the phenotypic, genotypic and phylogenetic evidence, strain OP-1(T) represents a novel species of the genus Burkholderia, for which the name Burkholderia zhejiangensis sp. nov. is proposed. The type strain is OP-1(T) ( = CCTCC AB 2010354(T) = KCTC 23300(T)).
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lu
authors.affiliation: Hualan Biological Engineering Inc., Xinxiang, Henan 453003, PR China.|The Key Laboratory for Microbial Resources of the Ministry of Education, People's Republic of China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Liu-Qiang
authors.initials: LQ
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Jin-Jin
authors.initials: JJ
authors.lastname: Sun
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Hong-Ming
authors.initials: HM
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.affiliation: The Key Laboratory for Microbial Resources of the Ministry of Education, People's Republic of China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D001482:Base Composition; D001673:Biodegradation, Environmental; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids; D007306:Insecticides / Q000378:metabolism*; D008743:Methyl Parathion / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D018508:Water Purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D008743:Methyl Parathion