title: Modified noninvasive microtest electrophysiological technology for vacuolar H(+) flux detection.
abstract: This paper describes a modified noninvasive microtest electrophysiological technology (NMT) for vacuolar H(+) flux detection. In this NMT system, the vacuole isolation procedure and buffer slope were modified, and the measuring errors from small spherical geometry were corrected. The trends in changes of vacuolar H(+) flux (ΔH(+) flux) after ATP or PP(i) supply calculated by NMT were consistent with the activities of V-ATPase and PPase measured by traditional methods. These findings indicate that our modified NMT is an appropriate method for vacuolar H(+) flux detection.
authors.affiliation: Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China.
authors.forename: Xianyang
authors.initials: X
authors.lastname: Chen
authors.forename: Lingling
authors.initials: L
authors.lastname: Nie
authors.forename: Hexigeduleng
authors.initials: H
authors.lastname: Bao
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Sulian
authors.initials: S
authors.lastname: Lv
authors.forename: Yinxin
authors.initials: Y
authors.lastname: Li
mesh_terms: D002021:Buffers; D004594:Electrophysiology / Q000379:methods*; D014026:Nicotiana / Q000201:enzymology / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases / Q000032:analysis* / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D002021:Buffers; D025262:Vacuolar Proton-Translocating ATPases