title: [Contents comparision of atractylode I, II, III in rhizoma atractylodis macrocephalae and the processed with soils].
abstract: OBJECTIVE
  To research the effect of processing with soils on the contents of Atractylode I, II, III of Rhizoma Atractylodis Macrocephalae.
  
  
  METHODS
  Contents of Atractylode I, II, III were determined by HPLC. Hypersil-ODS C18 (200 mm x 4.6 mm, 5 microm) column was used; The mobile phase consisted of acetonitrile-water; The flow rate was 1.0 mL/min; The detection wavelength was 220 nm and 276 nm,the column temperature was 30 degrees C.
  
  
  RESULTS
  All the indexes of Atractylode I, II, III peak were qualified; The result of methodological study was met the requirement of HPLC; The average contents of Atractylode I , II , II in raw Rhizoma Atractylodis Macrocephalae were 0.4365, 0.2878, 0.4140 mg/g, the soil-processed Rhizoma Atractylodis Macrocephalae were 0.5503, 0.3013, 0.8403 mg/g, the processed Rhizoma Atractylodis Macrocephalae were 0.5386, 0.2958, 0.7399 mg/g.
  
  
  CONCLUSIONS
  The content of Atractylode II is no significant difference among the raw Rhizoma Atractylodis Macrocephalae and the Processed. The contents of both Atractylode I and III increased after processed. However, there is no significant difference between the soil-processed and the processed Rhizoma Atractylodis Macrocephalae with nothing.
authors.affiliation: Key Lab of Chinese Medicinal Standardization of Ministry of Education, Chengdu University of TCM, Chengdu 610075, China. chen_hongping@126.com
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Chen
authors.forename: Jie-Hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D031174:Atractylodes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D006358:Hot Temperature; D007783:Lactones / Q000032:analysis* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D012987:Soil; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D007783:Lactones; D012987:Soil; D012997:Solvents