title: Development of a liquid chromatography-tandem mass spectrometric method for the simultaneous determination of tropane alkaloids and glycoalkaloids in crops.
abstract: A new, rapid and sensitive multiresidue method is reported for the simultaneous determination of tropane alkaloids (tropine, atropine, scopolamine, homatropine, anisodamine) and glycoalkaloids (α-solanine, α-chaconine) in grains and seeds (wheat, rye, maize, soybean, linseed). Dispersive solid phase extraction (DSPE) was performed with 0.5% formic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. For a fast and effective clean-up procedure for oily matrices such as soybean and linseed, matrix solid phase dispersion (MSPD) C(18) material was used to remove co-extracted non-polar components. No clean-up was necessary for less oily matrices following extraction. The analytes were separated by isocratic HPLC on a Chirobiotic V column and detected using a triple quadrupole mass spectrometer with electrospray ionization (ESI). All analytes were monitored in the positive ion mode. The method performance is presented in terms of linearity in the range 5-80 ng/g (r(2)=0.998), specifity, selectivity, accuracy (recoveries from 61-111%), precision (CV<5%) and ruggedness. The limits of quantitation (LOQ) were in the range 2.2-4.9 ng/g.
authors.affiliation: Food and Enviromental Protection Laboratory, FAO/IAEA Agriculture and Biotechnology Laboratories, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Wagramer Strasse 5, P.O. Box 100, 1400 Vienna, Austria.
authors.forename: Z
authors.initials: Z
authors.lastname: Jandrić
authors.forename: M N
authors.initials: MN
authors.lastname: Rathor
authors.forename: J
authors.initials: J
authors.lastname: Švarc-Gajić
authors.forename: B M
authors.initials: BM
authors.lastname: Maestroni
authors.forename: J J
authors.initials: JJ
authors.lastname: Sasanya
authors.forename: R
authors.initials: R
authors.lastname: Djurica
authors.forename: A
authors.initials: A
authors.lastname: Cannavan
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D002523:Edible Grain / Q000737:chemistry; D005506:Food Contamination; D015394:Molecular Structure; D012639:Seeds; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014326:Tropanes / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D014326:Tropanes