title: Brachypodium distachyon as a new model system for understanding iron homeostasis in grasses: phylogenetic and expression analysis of Yellow Stripe-Like (YSL) transporters.
abstract: OBJECTIVE
  Brachypodium distachyon is a temperate grass with a small stature, rapid life cycle and completely sequenced genome that has great promise as a model system to study grass-specific traits for crop improvement. Under iron (Fe)-deficient conditions, grasses synthesize and secrete Fe(III)-chelating agents called phytosiderophores (PS). In Zea mays, Yellow Stripe1 (ZmYS1) is the transporter responsible for the uptake of Fe(III)-PS complexes from the soil. Some members of the family of related proteins called Yellow Stripe-Like (YSL) have roles in internal Fe translocation of plants, while the function of other members remains uninvestigated. The aim of this study is to establish brachypodium as a model system to study Fe homeostasis in grasses, identify YSL proteins in brachypodium and maize, and analyse their expression profiles in brachypodium in response to Fe deficiency.
  
  
  METHODS
  The YSL family of proteins in brachypodium and maize were identified based on sequence similarity to ZmYS1. Expression patterns of the brachypodium YSL genes (BdYSL genes) were determined by quantitative RT-PCR under Fe-deficient and Fe-sufficient conditions. The types of PS secreted, and secretion pattern of PS in brachypodium were analysed by high-performance liquid chromatography.
  
  
  RESULTS
  Eighteen YSL family members in maize and 19 members in brachypodium were identified. Phylogenetic analysis revealed that some YSLs group into a grass-specific clade. The Fe status of the plant can regulate expression of brachypodium YSL genes in both shoots and roots. 3-Hydroxy-2'-deoxymugineic acid (HDMA) is the dominant type of PS secreted by brachypodium, and its secretion is diurnally regulated.
  
  
  CONCLUSIONS
  PS secretion by brachypodium parallels that of related crop species such as barley and wheat. A single grass species-specific YSL clade is present, and expression of the BdYSL members of this clade could not be detected in shoots or roots, suggesting grass-specific functions in reproductive tissues. Finally, the Fe-responsive expression profiles of several YSLs suggest roles in Fe homeostasis.
authors.affiliation: Biology Department, University of Massachusetts Amherst, 611 North Pleasant St., Amherst, MA 01002, USA
authors.forename: Burcu K
authors.initials: BK
authors.lastname: Yordem
authors.forename: Sarah S
authors.initials: SS
authors.lastname: Conte
authors.forename: Jian Feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Kengo
authors.initials: K
authors.lastname: Yokosho
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Vasques
authors.forename: Srinivasa N
authors.initials: SN
authors.lastname: Gopalsamy
authors.forename: Elsbeth L
authors.initials: EL
authors.lastname: Walker
mesh_terms: D058431:Brachypodium / Q000235:genetics* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006706:Homeostasis; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D010940:Plant Proteins; D007501:Iron