title: Genomic and EST microsatellite markers for Aquilegia flabellata and cross-amplification in A. oxysepala (Ranunculaceae).
abstract: OBJECTIVE
  The goal of this study was to identify genomic and expressed sequence tag (EST)-derived microsatellite markers from the species Aquilegia flabellata and assess their transferability in A. oxysepala.
  
  
  RESULTS
  Eleven genomic and nine EST-derived microsatellite loci were characterized in A. flabellata. These microsatellite primers amplified 66 alleles in all 20 loci. The observed heterozygosity (H(O)) for each population ranged from 0.00 to 0.94.
  
  
  CONCLUSIONS
  All of the 20 loci were successfully amplified in A. oxysepala. These genomic and EST-derived microsatellite markers will be useful in further genetic structure, speciation, and adaptive evolution studies.
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, P. R. China 130024.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Li
authors.forename: Di
authors.initials: D
authors.lastname: Pang
authors.forename: Qiang-Lan
authors.initials: QL
authors.lastname: Liao
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Xiao
mesh_terms: D000483:Alleles; D031884:Aquilegia / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D020224:Expressed Sequence Tags / Q000378:metabolism*; D005787:Gene Frequency; D006580:Genetic Carrier Screening / Q000379:methods; D056426:Genetic Loci; D018745:Genome, Plant; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant