title: [Effects of carbon monoxide inhalation on severe limb ischemia/reperfusion injury and its damages lead to shock].
abstract: OBJECTIVE
  To study the protective effect of carbon monoxide (CO) inhalation on the serious limb ischemia/reperfusion (I/R) injury, and which effects caused to shock in rats.
  
  
  METHODS
  36 SD rats were randomly divided into I/R, I/R + CO (RC), sham operation (S) groups. I/R injury models were made by the occlusion of the femoral artery for 8 h and the reperfusion for 12 h, 10 d. Before reperfusion of 2 h, RC group started to breathe medical air containing CO (the volume fraction of CO: 0.075%) continuously, until after reperfusion for 4 h, a total of inhalation 6 h. S, I/R groups exposed to air, breathe freely. Caudal artery pressures (CAP), ten days survival rate, serum lactate dehydrogenase (LDH) and creatine kinase (CK) activity, limb wet - to - dry weight ratio (W/D) and the pathologic changes of limb were observed.
  
  
  RESULTS
  Once the reperfusion started, the CAP decreased rapidly in I/R group, and the mean reduced to(5.3259 +/- 0.3832) kPa when reperfusion for 8 h. Compared to I/R group, the CAP decreased slower and smaller in RC group, moreover, its mean reduced to (8.3300 +/- 0.4224) kPa when reperfusion for 8 h. The 10 d survival rate in I/R group was that 8 rats died all between reperfusion for 13 - 20 h. Only 1 rat died in RC group and the other 7 rats were still alive when reperfusion for 10 d. Compared to I/R group, the pathological features of the ischemic limb were significant ly improved, and the figures of W/D, serum LDH and CK value were remarkable lower in RC group (P < 0.05).
  
  
  CONCLUSIONS
  Inhaling exogenous low-dose CO has a reverse regulation in the blood pressure decline caused by serious limb I/R injury in rats. And at the same time, it can effectively prevent the occurrence of shock, reduce physical damage, significantly increase the survival rate of animals.
authors.affiliation: Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Shi
authors.forename: Wan-Zheng
authors.initials: WZ
authors.lastname: Qi
authors.forename: Zhen-Long
authors.initials: ZL
authors.lastname: Zhu
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D002248:Carbon Monoxide / Q000008:administration & dosage / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D005121:Extremities / Q000098:blood supply*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D012769:Shock / Q000209:etiology / Q000517:prevention & control*
chemical_list: D002248:Carbon Monoxide; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase