title: In Vitro and In Vivo Pre-Clinical Analysis of a F(ab')(2) Fragment of Panitumumab for Molecular Imaging and Therapy of HER1 Positive Cancers.
abstract: BACKGROUND
  The objective of this study was to characterize the in vitro and in vivo properties of the F(ab')(2) fragment of panitumumab and to investigate its potential for imaging and radioimmunotherapy.
  
  
  METHODS
  The panitumumab F(ab')(2) was generated by enzymatic pepsin digestion. After the integrity and immunoreactivity of the F(ab')(2) was evaluated, the fragment was radiolabeled. In vivo studies included direct quantitation of tumor targeting and normal organ distribution of the radiolabeled panitumumab F(ab')(2) as well as planar γ-scintigraphy and PET imaging.
  
  
  RESULTS
  The panitumumab F(ab')(2) was successfully produced by peptic digest. The F(ab')(2) was modified with the CHX-A"-DTPA chelate and efficiently radiolabeled with either (111)In or (86)Y. In vivo tumor targeting was achieved with acceptable uptake of radioactivity in the normal organs. The tumor targeting was validated by both imaging modalities with good visualization of the tumor at 24 h.
  
  
  CONCLUSIONS
  The panitumumab F(ab')(2) fragment is a promising candidate for imaging of HER1 positive cancers.
authors.affiliation: Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda MD 20892; kwong@mail.nih.gov.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Wong
authors.forename: Kwamena E
authors.initials: KE
authors.lastname: Baidoo
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Nayak
authors.forename: Kayhan
authors.initials: K
authors.lastname: Garmestani
authors.forename: Martin W
authors.initials: MW
authors.lastname: Brechbiel
authors.forename: Diane E
authors.initials: DE
authors.lastname: Milenic