title: Development of a novel aptamer-based sensing system using atomic force microscopy.
abstract: Atomic force microscopy (AFM) can dynamically detect the adhesion or affinity force between a sample surface and a cantilever. This feature is useful as a detection method using aptamers--single-strand DNA that recognizes its target with very high affinity. The present study proposes a novel DNA aptamer-based sensing system using AFM. In this study, thrombin was chosen as the target molecule, and a DNA aptamer-based AFM sensing system based on competition was developed. The affinity force between the gold chip and the cantilever decreased as the concentration of thrombin increased. Moreover, a low detection limit of 0.2 nM was achieved. Therefore, the AFM sensing system used would be appropriate for the measurement of various chemical compounds.
authors.affiliation: Department of Chemical Science and Engineering, Faculty of Engineering, Graduate School of Engineering, Kobe University, Rokkoudai-chou 1-1, Nada, Kobe 657-8501, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyachi
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Amino
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry; D013917:Thrombin / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D001710:Biotin; D006046:Gold; D019809:Streptavidin; D013917:Thrombin