title: Adaptation to the chicken intestine in Salmonella Enteritidis PT4 studied by transcriptional analysis.
abstract: The transcriptional changes that occurred in Salmonella enterica serovar Enteritidis during colonization of the alimentary tract of newly hatched chickens were studied. A whole genome oligonucleotide microarray was used to compare the expression pattern with that from bacteria cultured in nutrient broth in vitro. Amongst other changes Salmonella Pathogenicity Island (SPI)-1, SPI-2 and SPI-5 genes were up-regulated in vivo suggesting a close association with the mucosa during colonization. Particular attention was paid to genes associated with metabolism of dicarboxylic acids and to responses to high osmolarity. Association between the colonization phenotype and gene mutations indicated that the latter was more important as a contribution to the colonization phenotype.
authors.affiliation: School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Dhawi
authors.forename: A
authors.initials: A
authors.lastname: Elazomi
authors.forename: M A
authors.initials: MA
authors.lastname: Jones
authors.forename: M A
authors.initials: MA
authors.lastname: Lovell
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: R D
authors.initials: RD
authors.lastname: Emes
authors.forename: P A
authors.initials: PA
authors.lastname: Barrow
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D020869:Gene Expression Profiling; D044404:Genomic Islands; D007413:Intestinal Mucosa / Q000382:microbiology*; D009154:Mutation; D011201:Poultry Diseases / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins