title: In vitro identification of the human cytochrome p450 enzymes involved in the oxidative metabolism of loxapine.
abstract: In vitro studies were conducted to identify the hepatic cytochrome P450 (CYP) enzymes responsible for the oxidative metabolism of loxapine to 8-hydroxyloxapine, 7-hydroxyloxapine, N-desmethylloxapine (amoxapine) and loxapine N-oxide. These studies included use of cDNA-expressed enzymes, correlation analysis with 12 phenotyped human liver microsomal samples, and use of selective inhibitors of cytochrome P450s. The resultant data indicated that loxapine was mainly metabolized by human liver microsomes to (i) 8-hydroxyloxapine by CYP1A2, (ii) 7-hydroxyloxapine by CYP2D6, (iii) N-desmethyloxapine by CYP3A4 and (iv) loxapine N-oxide by CYP3A4. The involvement of flavin-containing monooxygenase (FMO) in the formation of loxapine N-oxide was also observed.
authors.affiliation: College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Jiang Ping
authors.initials: JP
authors.lastname: Luo
authors.forename: Sarvesh C
authors.initials: SC
authors.lastname: Vashishtha
authors.forename: Edward M
authors.initials: EM
authors.lastname: Hawes
authors.forename: Gordon
authors.initials: G
authors.lastname: McKay
authors.forename: Kamal K
authors.initials: KK
authors.lastname: Midha
authors.forename: Jim
authors.initials: J
authors.lastname: Fang
mesh_terms: D014150:Antipsychotic Agents / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008152:Loxapine / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction
chemical_list: D014150:Antipsychotic Agents; D003577:Cytochrome P-450 Enzyme System; D008152:Loxapine