title: Quantitative comparison of the expression of antimicrobial peptides in the oral mucosa and extraoral skin.
abstract: Antimicrobial peptides (AMP) defend epithelial surfaces against pathological micro-organisms. We know of no comparison of their expression between the oral mucosa and extraoral epithelium, but knowledge of differences in their quantities is of interest, possibly as a starting point for new treatments. Expression of AMP human beta-defensin (hBD)-1/-2/-3 and psoriasin in the oral mucosa and extraoral epithelium of the head and neck were measured by real-time polymerase chain reaction (RT-PCR) (n=14), immunohistochemistry (n=6), and western blot (n=8). RT-PCR showed that all the genes investigated were expressed significantly more in the oral mucosa than in the skin (hBD-1: p=0.002; hBD-2: p=0.006; hBD-3: p=0.035; psoriasin: p=0.02). Immunohistochemistry and western blot showed differential concentrations of proteins: hBD-2 (p=0.021) and hBD-3 (p=0.043) were pronounced in the oral mucosa, whereas psoriasin was raised in the extraoral skin (p=0.021). There was no difference in protein concentrations for hBD-1 (p=0.08). The observed differences in the expression of AMP may be important for new treatments such as topical application of AMP derivatives.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Technische Universität München, Ismaninger Str. 22, D-81675 Munich, Germany.
authors.forename: Marco R
authors.initials: MR
authors.lastname: Kesting
authors.forename: Christian
authors.initials: C
authors.lastname: Mueller
authors.forename: Stefan
authors.initials: S
authors.lastname: Wagenpfeil
authors.forename: Mechthild
authors.initials: M
authors.lastname: Stoeckelhuber
authors.forename: Timm
authors.initials: T
authors.lastname: Steiner
authors.forename: Florian
authors.initials: F
authors.lastname: Bauer
authors.forename: Jan
authors.initials: J
authors.lastname: Teichmann
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Baumann
authors.forename: Leopold C
authors.initials: LC
authors.lastname: Barthel
authors.forename: Robin M
authors.initials: RM
authors.lastname: Satanovskij
authors.forename: Thomas
authors.initials: T
authors.lastname: Mücke
authors.forename: Matthias
authors.initials: M
authors.lastname: Schulte
authors.forename: Katrin
authors.initials: K
authors.lastname: Schütz
authors.forename: Klaus-D
authors.initials: KD
authors.lastname: Wolff
authors.forename: Nils H
authors.initials: NH
authors.lastname: Rohleder
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D002648:Child; D004848:Epithelium / Q000276:immunology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007113:Immunity, Innate; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000276:immunology / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D018709:Statistics, Nonparametric
chemical_list: D023181:Antimicrobial Cationic Peptides