title: Development and characterization of polymorphic microsatellite primers in Reaumuria soongorica (Tamaricaceae).
abstract: OBJECTIVE
  Polymorphic microsatellite markers were developed in Reaumuria soongorica, a desert shrub occurring widely in western China, to investigate its genetic diversity and population genetic structure.
  
  
  RESULTS
  Using the combined biotin capture technique, 10 novel polymorphic microsatellite loci primer sets were isolated and characterized in 90 individuals from six populations of R. soongorica. Five to 14 alleles per locus were identified for these microsatellites. Observed and expected heterozygosities ranged from 0.132 to 0.593, and 0.217 to 0.803, respectively.
  
  
  CONCLUSIONS
  These microsatellite markers provide a useful tool to assess the genetic variation and population structure of R. soongorica.
authors.affiliation: School of Life Science, North University for Nationalities, Yinchuan 750021, China. haikui2000@hotmail.com
authors.forename: Haikui
authors.initials: H
authors.lastname: Chen
authors.forename: Lili
authors.initials: L
authors.lastname: Zhu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
authors.forename: Dandan
authors.initials: D
authors.lastname: Song
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhong
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002681:China; D017931:DNA Primers / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005787:Gene Frequency; D006580:Genetic Carrier Screening / Q000379:methods; D056426:Genetic Loci; D018745:Genome, Plant; D006579:Heterozygote; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic; D032363:Tamaricaceae / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant