title: Anorexigenic action of nitric oxide synthase inhibition in the raphe nuclei.
abstract: The present report examined the effects of midbrain raphe nuclei injections of nitric oxide synthase inhibitors NG-nitro-L-arginine methyl ester (L-NAME) and 7-nitroindazole (7-NI) on eating behavior. L-NAME (5-500 pmol) and 7-NI (2-200 pmol) were administered into either the dorsal or median raphe nucleus. Both nitric oxide synthase inhibitors decreased food intake in adult male Sprague-Dawley rats when injected into either raphe site. Further, eating elicited by dorsal and median raphe injections of the 5-HT1A agonist 8-OH-DPAT (0.8 nmol) was attenuated by L-NAME or 7-NI pretreatment. Our findings indicate that nitric oxide acts within the raphe to alter food intake. The inhibitory effects of L-NAME and 7-NI on eating elicited by 8-OH-DPAT further suggest that nitric oxide and 5-HT systems interact in the control of food intake.
authors.affiliation: Department of Psychology, Reed College, 3203 SE Woodstock Blvd, Portland, Oregon 97202, USA. pcurrie@reed.edu
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Currie
authors.forename: Aaisha
authors.initials: A
authors.lastname: Mirza
authors.forename: Lindsey M
authors.initials: LM
authors.lastname: Dono
authors.forename: Catherine S
authors.initials: CS
authors.lastname: John
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Wall
mesh_terms: D000818:Animals; D001067:Appetite Depressants / Q000008:administration & dosage*; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology; D007276:Injections, Intraventricular; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011897:Random Allocation; D011903:Raphe Nuclei / Q000187:drug effects* / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001067:Appetite Depressants; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester