title: Dynamics of biomolecules from picoseconds to seconds at atomic resolution.
abstract: Although biomolecular dynamics has been investigated using NMR for at least 40 years, only in the past 20 years have internal motions been characterized at atomic resolution throughout proteins and nucleic acids. This development was made possible by multidimensional heteronuclear NMR approaches that provide near complete sequential signal assignments of uniformly labeled biomolecules. Recent methodological advances have enabled characterization of internal dynamics on timescales ranging from picoseconds to seconds, both in solution and in the solid state. The size, complexity and functional significance of biomolecules investigated by NMR continue to grow, as do the insights that have been obtained about function. In this article I review a number of recent advances that have made such studies possible, and provide a few examples of where NMR either by itself or in combination with other approaches has paved the way to a better understanding of the complex relationship between dynamics and biomolecular function. Finally, I discuss prospects for further advances in this field.
authors.affiliation: NIH, Building 5, Room 128, Bethesda, MD 20892-0520, USA. dtorchia@dir.nidcr.nih.gov
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Torchia
mesh_terms: D000465:Algorithms; D016880:Anisotropy; D004574:Electromagnetic Fields; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011522:Protons
chemical_list: D009696:Nucleic Acids; D011506:Proteins; D011522:Protons