title: Benzo(a)pyrene-induced metabolic responses in Manila clam Ruditapes philippinarum by proton nuclear magnetic resonance ((1)H NMR) based metabolomics.
abstract: Benzo(a)pyrene is an important polycyclic aromatic hydrocarbon (PAH) which causes carcinogenic, teratogenic and mutagenic effects in various species and the level of contamination of this toxic agent in the marine environment is of great concern. In this study, metabolic responses induced by two doses (0.02 and 0.2μM) of BaP were characterized in the gill tissues of Manila clam Ruditapes philippinarum after exposure for 24, 48 and 96h. The high dose (0.2μM) of BaP induced the disturbances in energy metabolism and osmotic regulation based on the metabolic biomarkers such as succinate, alanine, glucose, glycogen, branched chain amino acids, betaine, taurine, homarine, and dimethylamine in clam gills after 24h of exposure. In addition, hormesis induced by BaP was found in clams exposed to both doses of BaP. Overall, our results demonstrated the applicability of metabolomics for the elucidation of toxicological effects of marine environmental contaminants in a selected bioindicator species such as the Manila clam.
authors.affiliation: Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
authors.forename: Linbao
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: You
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Cong
authors.forename: Lianzhen
authors.initials: L
authors.lastname: Li
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Dongyan
authors.initials: D
authors.lastname: Liu
authors.forename: Junbao
authors.initials: J
authors.lastname: Yu
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D049872:Bivalvia / Q000033:anatomy & histology / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects; D005880:Gills / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D025341:Principal Component Analysis; D011897:Random Allocation; D014020:Tissue Extracts / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D014020:Tissue Extracts; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene