title: Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer.
abstract: Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.
authors.affiliation: Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tamaki
authors.forename: Michiro
authors.initials: M
authors.lastname: Otaka
authors.forename: Tomoyoshi
authors.initials: T
authors.lastname: Shibuya
authors.forename: Naoto
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Soh
authors.initials: S
authors.lastname: Yamamoto
authors.forename: Masaru
authors.initials: M
authors.lastname: Odashima
authors.forename: Hideaki
authors.initials: H
authors.lastname: Itoh
authors.forename: Sumio
authors.initials: S
authors.lastname: Watanabe