title: A bias caused by ectopic development produces sexually dimorphic sperm in nematodes.
abstract: Self-fertile hermaphrodites have evolved independently several times in the genus Caenorhabditis [1, 2]. These XX hermaphrodites make smaller sperm than males [3, 4], which they use to fertilize their own oocytes. Because larger sperm outcompete smaller sperm in nematodes [3-5], it had been assumed that this dimorphism evolved in response to sperm competition. However, we show that it was instead caused by a developmental bias. When we transformed females of the species Caenorhabditis remanei into hermaphrodites [6], their sperm were significantly smaller than those of males. Because this species never makes hermaphrodites in the wild, this dimorphism cannot be due to selection. Instead, analyses of the related nematode Caenorhabditis elegans suggest that this dimorphism might reflect the development of sperm within the distinct physiological environment of the hermaphrodite gonad. These results reveal a new mechanism for some types of developmental bias-the effects of a novel physical location alter the development of ectopic cells in predictable ways.
authors.affiliation: Graduate School of Biomedical Sciences, The UMDNJ School of Osteopathic Medicine, B303 Science Center, 2 Medical Center Drive, Stratford, NJ 08084, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Baldi
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Viviano
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Ellis
mesh_terms: D000818:Animals; D005075:Biological Evolution; D002107:Caenorhabditis / Q000145:classification / Q000166:cytology* / Q000254:growth & development*; D012734:Disorders of Sex Development; D005260:Female; D007814:Larva / Q000033:anatomy & histology / Q000502:physiology; D008297:Male; D010802:Phylogeny; D034622:RNA Interference; D012727:Sex Characteristics; D019849:Sex Determination Processes; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*