title: Detection of matrix metalloproteinase (MMP)-2 and MMP-9 in canine seminal plasma.
abstract: Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that play a central role in degradation of protein components of the extracellular matrix and basement membrane. Previous studies have shown that MMP-2 and MMP-9 are present in human seminal plasma, but there is little information available on the presence of MMPs in canine seminal plasma. This study aims to investigate the presence of MMPs in canine seminal plasma and their clinical manifestation at the level of various semen parameters in canine species. Latent and active forms of MMP-2 and MMP-9 were evaluated using gelatin zymography and their association with semen parameters was examined. Results demonstrate that both latent and active forms of MMP-2 and MMP-9 are present in canine seminal plasma and the latent forms are predominant. The latent and active MMP-9 activities were elevated in the semen with unsatisfactory quality traits and proMMP-2 was inversely correlated with semen quality whereas, MMP-2 was positively correlated with semen quality traits. These findings suggest that proMMP-9 and MMP-9 activation contributes to the variation in semen, while the activation of MMP-2 improves the sperm functionality.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
authors.forename: Wipawee
authors.initials: W
authors.lastname: Saengsoi
authors.forename: Wei-Yau
authors.initials: WY
authors.lastname: Shia
authors.forename: Ching-Lin
authors.initials: CL
authors.lastname: Shyu
authors.forename: Jui-Te
authors.initials: JT
authors.lastname: Wu
authors.forename: Chuleekorn
authors.initials: C
authors.lastname: Warinrak
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Lee
authors.forename: Feng-Pang
authors.initials: FP
authors.lastname: Cheng
mesh_terms: D000818:Animals; D004285:Dogs / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012661:Semen / Q000201:enzymology*; D013076:Sperm Count / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9