title: Separation of platelets from whole blood using standing surface acoustic waves in a microchannel.
abstract: Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.
authors.affiliation: School of Mechanical Engineering, Korea University, 136-713 Seoul, Korea.
authors.forename: Jeonghun
authors.initials: J
authors.lastname: Nam
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Lim
authors.forename: Dookon
authors.initials: D
authors.lastname: Kim
authors.forename: Sehyun
authors.initials: S
authors.lastname: Shin
mesh_terms: D000162:Acoustics / Q000295:instrumentation*; D001792:Blood Platelets / Q000502:physiology*; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005434:Flow Cytometry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon