title: Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen.
abstract: We report the detailed studies on the inhibitory effect of tannic acid (TA) on Clostridium histolyticum collagenase (ChC) activity against degradation of extracellular matrix component of collagen. The TA treated collagen exhibited 64% resistance against collagenolytic hydrolysis by ChC, whereas direct interaction of TA with ChC exhibited 99% inhibition against degradation of collagen and the inhibition was found to be concentration dependant. The kinetic inhibition of ChC has been deduced from the extent of hydrolysis of N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala (FALGPA). This data provides a selective competitive mode of inhibition on ChC activity seems to be influenced strongly by the nature and structure of TA. TA showed inhibitor activity against the ChC by molecular docking method. This result demonstrated that TA containing digalloyl radical possess the ability to inhibit the ChC. The inhibition of ChC in gaining new insight into the mechanism of stabilization of collagen by TA is discussed.
authors.affiliation: Bioproducts Laboratory-Biomaterial Development Division, Central Leather Research Institute (Council of Scientific & Industrial Research), Chennai, Tamil Nadu, India.
authors.forename: Ganesan
authors.initials: G
authors.lastname: Krishnamoorthy
authors.forename: Praveen Kumar
authors.initials: PK
authors.lastname: Sehgal
authors.forename: Asit Baran
authors.initials: AB
authors.lastname: Mandal
authors.forename: Sayeed
authors.initials: S
authors.lastname: Sadulla
mesh_terms: D047009:Hathewaya histolytica / Q000201:enzymology; D003094:Collagen / Q000737:chemistry / Q000378:metabolism*; D017364:Collagenases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005109:Extracellular Matrix / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D008958:Models, Molecular; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D013634:Tannins / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D013634:Tannins; D003094:Collagen; D017364:Collagenases