title: Alterations in peripheral blood B-cell populations in SHIV89.6P-infected macaques (Macacca fascicularis).
abstract: In addition to CD4+ T cell depletion, the B cell compartment of HIV-infected patients exhibits abnormalities, including deficits and diminished responses to ex vivo antigenic stimulation and in vivo vaccination. We used chimeric simian-human immunodeficiency virus (SHIV) infection of cynomolgus macaques to determine the dynamics of peripheral blood B cell alterations in this model of HIV infection. During the course of infection, we observed progressive loss of total and memory (CD27+) B cells, increased percentages of activated (CD95+) B cells, hypergammaglobulinemia, and deficits in the CD21+ B cell population. In addition, we noted declines in subsets of memory B cells, including both IgM+ and class-switched (IgD-IgM- CD27+) cells, with sustained deficits in the IgM+ memory (IgM+CD27+) B cell population. The similarity of the B cell alterations in these studies to those observed in HIV+ subjects supports the utility of the SHIV macaque model for examination of HIV-related B cell dysfunction.
authors.affiliation: Department of Immunology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Heather M
authors.initials: HM
authors.lastname: Kling
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Shipley
authors.forename: Karen A
authors.initials: KA
authors.lastname: Norris
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology* / Q000502:physiology / Q000821:virology; D018791:CD4 Lymphocyte Count; D004195:Disease Models, Animal; D015658:HIV Infections / Q000276:immunology; D006942:Hypergammaglobulinemia / Q000175:diagnosis; D007074:Immunoglobulin G / Q000097:blood; D007156:Immunologic Memory; D018655:Lymphocyte Count; D008212:Lymphocyte Depletion; D008252:Macaca fascicularis / Q000821:virology; D017464:Receptors, Complement 3d / Q000378:metabolism; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus; D019562:Viral Load; D019014:fas Receptor / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D017464:Receptors, Complement 3d; D019014:fas Receptor