title: [Study on yohimbine in positive mode by ion trap mass spectrometry].
abstract: OBJECTIVE
  The structure and fragmentation pathway of yohimbine were elucidated by electron spray ionization mass spectrometry( ESI-MS).
  
  
  METHODS
  Quasi-molecular ion peak m/z 355 [M + H]+ was detected by ESI-MS, and the main fragment ions of m/z 212 and m/z 144 were detected by ESI-MS2.
  
  
  RESULTS
  There are two main fragment pathway for m/z 355 [M + H]+ by ESI-MS2 and the fragment broken in pyridine ring. The full scan MS3 spectra of fragment m/z 212, and m/z 144 was obtained by ion trap mass spectrometry. The characteristic fragmentation was used to prove the structure of m/z 212, and m/z 144. The fragment routes of characteristic were discussed on the basis of ESI mass spectra.
  
  
  CONCLUSIONS
  It can provide the experimental data for studying pharmacokinetics in vivo and modifying structure.
authors.affiliation: Affiliated Dongfeng Hospital, Hubei Medical University, Shiyan 442008, China. dfyylp@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Qin-hua
authors.initials: QH
authors.lastname: Chen
authors.forename: Fu-chao
authors.initials: FC
authors.lastname: Chen
mesh_terms: D015394:Molecular Structure; D008970:Molecular Weight; D024301:Plant Bark / Q000737:chemistry; D019666:Rubiaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods; D015016:Yohimbine / Q000032:analysis / Q000737:chemistry*
chemical_list: D015016:Yohimbine