title: Hydrogen peroxide sensors for cellular imaging based on horse radish peroxidase reconstituted on polymer-functionalized TiO₂ nanorods.
abstract: We describe the reconstitution of apo-horse radish peroxidase (apo-HRP) onto TiO(2) nanorods functionalized with a multifunctional polymer. After functionalization, the horse radish peroxidase (HRP) functionalized TiO(2) nanorods were well dispersible in aqueous solution, catalytically active and biocompatible, and they could be used to quantify and image H(2)O(2) which is a harmful secondary product of cellular metabolism. The shape, size and structure of TiO(2) nanorods (anatase) were analyzed by transmission electron microscopy (TEM), high resolution TEM (HRTEM), electron diffraction (ED) and X-ray diffraction (XRD). The surface functionalization, HRP reconstitution and catalytic activity were confirmed by UV-Vis, FT-IR, CLSM and atomic force microscopy (AFM). Biocompatibility and cellular internalization of active HRP reconstituted TiO(2) nanorods were confirmed by a classical MTT cytotoxicity assay and confocal laser scanning microscopy (CLSM) imaging, respectively. The intracellular localization allowed H(2)O(2) detection, imaging and quantification in HeLa cells. The polymer functionalized hybrid system creates a complete sensor including a "cell positioning system" in each single particle. The flexible synthetic concept with functionalization by post-polymerization modification allows introduction of various dyes for sensitisation at different wavelengths and introduction of various anchor groups for anchoring on different particles.
authors.affiliation: Institut für Anorganische Chemie, Johannes Gutenberg-Universität, Duesbergweg 10-14, D-55099 Mainz, Germany. tahir@uni-mainz.de
authors.forename: Muhammad Nawaz
authors.initials: MN
authors.lastname: Tahir
authors.forename: Rute
authors.initials: R
authors.lastname: André
authors.forename: Jugal Kishore
authors.initials: JK
authors.lastname: Sahoo
authors.forename: Florian D
authors.initials: FD
authors.lastname: Jochum
authors.forename: Patrick
authors.initials: P
authors.lastname: Theato
authors.forename: Filipe
authors.initials: F
authors.lastname: Natalio
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Berger
authors.forename: Robert
authors.initials: R
authors.lastname: Branscheid
authors.forename: Ute
authors.initials: U
authors.lastname: Kolb
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Tremel
mesh_terms: D006367:HeLa Cells; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D018613:Microscopy, Confocal / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D011108:Polymers; C009495:titanium dioxide; D006861:Hydrogen Peroxide; D014025:Titanium; D006735:Horseradish Peroxidase