title: Fluorescent detection of ATP based on signaling DNA aptamer attached silica nanoparticles.
abstract: Novel methods for rapid, sensitive and low-cost biomolecule detection have attracted particular interest because of their wide use in medical diagnostics, food inspection and biomedical research applications. In this work, we report a simple and efficient silica nanoparticle (NP)-based fluorescent assay for ATP detection. It takes advantage of the washing and separation properties of NPs and the structure-switch property of DNA aptamers, resulting in fluorescence change of the supernatant in the presence of targets. A linear response for ATP detection was observed from 0 to 6 mM with a detection limit of ∼34 µM. This detection strategy could be generalized to other aptamer-based detection systems.
authors.affiliation: Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117567, Singapore.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yusong
authors.initials: Y
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu