title: Stable cellulose nanospheres for cellular uptake.
abstract: Pure, perfectly spherical cellulose nanoparticles with sizes of ≈80-260 nm can be prepared by dialysis starting from trimethylsilylcellulose (TMSC). The aqueous suspensions obtained are storable for several months. Subsequent covalent labeling of the cellulose nanoparticles with FITC has no influence on particle size, shape, and stability. The particles can be sterilized and suspended in biological media without structural changes. Incorporation of FITC-labeled cellulose nanoparticles into living human fibroblasts is studied using confocal LSM. In contrast to cellulose nanocrystals, fast cellular uptake is found for the nanospheres without transfection reagents or attachment of a receptor molecule. This suggests an influence of the geometry of biocompatible nanomaterials on endocytosis.
authors.affiliation: Center of Excellence for Polysaccharide Research, Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University of Jena, Humboldtstrasse 10, D-07743 Jena, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Liebert
authors.forename: Marc
authors.initials: M
authors.lastname: Kostag
authors.forename: Jana
authors.initials: J
authors.lastname: Wotschadlo
authors.forename: Thomas
authors.initials: T
authors.lastname: Heinze
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry*; D003956:Dialysis; D004705:Endocytosis / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D057446:Hydrodynamics; D008027:Light; D008297:Male; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D012542:Scattering, Radiation; D013194:Staining and Labeling
chemical_list: D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D002482:Cellulose; D016650:Fluorescein-5-isothiocyanate