title: Evaluation of the association between GHR exon 3 polymorphism and polycystic ovary syndrome among Han Chinese women.
abstract: BACKGROUND
  Polycystic ovary syndrome (PCOS) and type 2 diabetes (T2D) are two common metabolic disorders in reproductive-aged women, and both are associated with insulin resistance. Evidence has indicated that the growth hormone receptor (GHR) exon 3 polymorphism is associated with T2D and the GHRd3 allele may have the preventive effect on the disease. However, the genetic effect of this polymorphism on PCOS is unknown. The present study thus aims to evaluate the association between the GHR exon 3 polymorphism and PCOS.
  
  
  METHODS
  A total of 432 patients with PCOS and 441 healthy control subjects were included. All of them were Han Chinese women and well characterized. Genotyping experiments of GHR exon 3 polymorphism were performed with a standard protocol of PCR and gel electrophoresis.
  
  
  RESULTS
  GHRd3 allele frequency in PCOS patients was significantly higher compared to the control subjects (19.1% vs. 14.3%; P=0.007, OR=1.416; 95% CI=1.099-1.825). Further analyses indicated that the GHRd3 allele was associated with increased waist and hip circumstance in healthy women (P=0.016; 0.003), and also with 1-h, 2-h and area under the curve (AUC) plasma glucose levels among PCOS patients (all P<0.05). But, no association of GHR exon 3 polymorphism with insulin resistance in the patients was observed.
  
  
  CONCLUSIONS
  The present study provides the first evidence that GHR exon 3 polymorphism is associated with PCOS in Han Chinese women. The GHRd3 allele may contribute to an impact of glucose metabolism but not insulin resistance.
authors.affiliation: Central laboratory, Provincial Hospital Affiliated to Shandong University, Jinan, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shen
authors.forename: Laicheng
authors.initials: L
authors.lastname: Wang
authors.forename: Yueran
authors.initials: Y
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: You
authors.forename: Ling
authors.initials: L
authors.lastname: Geng
authors.forename: Harvest F
authors.initials: HF
authors.lastname: Gu
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000235:genetics; D016022:Case-Control Studies; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D011110:Polymorphism, Genetic; D011986:Receptors, Somatotropin / Q000235:genetics*; D055105:Waist Circumference / Q000235:genetics
chemical_list: D001786:Blood Glucose; D011986:Receptors, Somatotropin