title: Deletion mapping and paternal origin of a Mexican AMELY negative male.
abstract: The amelogenin represents the gender marker most widely used for human identification and biomedical purposes. However, some failures in sex-typing have been observed globally. In this study, we could approximate the population frequency of AMELY negative males in 1230 individuals from five states of Mexico (0.081%). For the sole AMELY negative male detected, we constructed a deletion map by means of 10 markers (7 STS and 3 Y-STRs). This allowed classifying the case into the most common category (Class I deletion), according to the nomenclature proposed by Jobling et al. (2007). Interestingly, the Mexican sample was R1a1(∗), a Y-chromosome haplogroup non-previously reported for AMELY negative cases. The geographic distribution of R1a1(∗), and the Y-STR haplotype similarity with a reported case from Slovenia, suggests an Eastern-Europe paternal origin for this case from Mexico. To our knowledge, this is the first report in Latin America that implies a low population frequency and European paternal origin of AMELY negative cases.
authors.affiliation: Centro de Medicina Genómica del Hospital General de Culiacán "Dr. Bernardo J. Gastélum", Servicios de Salud de Sinaloa, Culiacán, Sinaloa, Mexico.
authors.forename: J S
authors.initials: JS
authors.lastname: Velarde-Félix
authors.forename: J
authors.initials: J
authors.lastname: Salazar-Flores
authors.forename: G
authors.initials: G
authors.lastname: Martínez-Cortés
authors.forename: A
authors.initials: A
authors.lastname: Flores García
authors.forename: J F
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.forename: J J
authors.initials: JJ
authors.lastname: Ríos-Tostado
authors.forename: R
authors.initials: R
authors.lastname: Rubi-Castellanos
authors.forename: H
authors.initials: H
authors.lastname: Rangel-Villalobos
mesh_terms: D000328:Adult; D053523:Amelogenin / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D005260:Female; D005828:Genetics, Population; D006801:Humans; D008800:Mexico / Q000208:ethnology; D010334:Paternity
chemical_list: C507202:AMELY protein, human; D053523:Amelogenin