title: The advantages of using a combination of LDL and glutamine in comparison with TRIS egg yolk and Equex® STAMP extenders in the cryopreservation of canine semen.
abstract: Twenty semen samples taken from 5 dogs were frozen in liquid nitrogen at -196 °C in four different extenders: one control extender based on 20% egg yolk, 6% LDL alone (low density lipoproteins: the active cryoprotective principle in chicken egg yolk), 6% LDL combined with 20 mmol glutamine, and Equex® (a reference extender that we wish to compare with the LDL-glutamine combination). After thawing, spermatozoal motility was evaluated using a HAMILTON THORNE CERROS 12 image analyzer; the percentage of motile spermatozoa was 27.7% in the egg yolk extender (p<0.05), 49.9% with 6% LDL alone (p>0.05), 54.7% in the 6% LDL+20 mmol glutamine extender, and 47.9% with Equex® (p>0.05). The motility parameters (VAP, VCL, VSL and ALH) were also superior in the 6% LDL+20 mmol glutamine extender in comparison with the other extenders. Finally, the spermatozoa were generally better protected during freezing with the 6% LDL+20 mmol glutamine association than with the egg yolk, 6% LDL, or Equex extenders in terms of the flagellar plasma membrane (HOS test), DNA (Acridine orange test), and acrosome integrity (Spermac® test: no significant difference). The Equex® extender obtained the best results for the acrosome, followed by 6% LDL+20 mmol glutamine (FITC-PSA test: p<0.05 between each extender).
authors.affiliation: Laboratory of Biotechnology and Pathology of Reproduction, Oniris, Ecole Nationale Vétérinaire, Agroalimentaire et de l'Alimentation, BP 40706, 44307 Nantes, France. djemil.bencharif@oniris-nantes.fr
authors.forename: Djemil
authors.initials: D
authors.lastname: Bencharif
authors.forename: Lamia
authors.initials: L
authors.lastname: Amirat-Briand
authors.forename: Annabelle
authors.initials: A
authors.lastname: Garand
authors.forename: Marc
authors.initials: M
authors.lastname: Anton
authors.forename: Eric
authors.initials: E
authors.lastname: Schmitt
authors.forename: Serge
authors.initials: S
authors.lastname: Desherces
authors.forename: Guy
authors.initials: G
authors.lastname: Delhomme
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Langlois
authors.forename: Paul
authors.initials: P
authors.lastname: Barrière
authors.forename: Sandrine
authors.initials: S
authors.lastname: Destrumelle
authors.forename: Oscar
authors.initials: O
authors.lastname: Vera-Munoz
authors.forename: Daniel
authors.initials: D
authors.lastname: Tainturier
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D004285:Dogs; D004530:Egg Yolk; D005973:Glutamine / Q000627:therapeutic use; D008077:Lipoproteins, LDL / Q000627:therapeutic use; D008297:Male; D012661:Semen / Q000187:drug effects / Q000378:metabolism; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D008077:Lipoproteins, LDL; D005973:Glutamine