title: Molecular wipes: application to epidemic keratoconjuctivitis.
abstract: Epidemic keratoconjunctivitis (EKC) is a severe disease of the eye, caused by members of the Adenoviridae (Ad) family, with symptoms such as keratitis, conjunctivitis, pain, edema, and reduced vision that may last for months or years. There are no vaccines or antiviral drugs available to prevent or treat EKC. It was found previously that EKC-causing Ads use sialic acid as a cellular receptor and demonstrated that soluble, sialic acid-containing molecules can prevent infection. In this study, multivalent sialic acid constructs based on 10,12-pentacosadiynoic acid (PDA) have been synthesized, and these constructs are shown to be efficient inhibitors of Ad binding (IC(50) = 0.9 μM) and Ad infectivity (IC(50) = 0.7 μM). The mechanism of action is to aggregate virus particles and thereby prevent them from binding to ocular cells. Such formulations may be used for topical treatment of adenovirus-caused EKC.
authors.affiliation: Center for Analysis and Synthesis, Chemical Center, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Karolina
authors.initials: K
authors.lastname: Aplander
authors.forename: Marko
authors.initials: M
authors.lastname: Marttila
authors.forename: Sophie
authors.initials: S
authors.lastname: Manner
authors.forename: Niklas
authors.initials: N
authors.lastname: Arnberg
authors.forename: Olov
authors.initials: O
authors.lastname: Sterner
authors.forename: Ulf
authors.initials: U
authors.lastname: Ellervik
mesh_terms: D000256:Adenoviridae / Q000187:drug effects*; D000258:Adenovirus Infections, Human / Q000188:drug therapy*; D000480:Alkynes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology; D058872:Epidemics; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000821:virology; D006801:Humans; D007637:Keratoconjunctivitis / Q000188:drug therapy* / Q000821:virology; D008081:Liposomes; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012794:Sialic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014771:Virion / Q000187:drug effects
chemical_list: D000480:Alkynes; D000998:Antiviral Agents; D008081:Liposomes; D012794:Sialic Acids; D011092:Polyethylene Glycols