title: Development of polymorphic microsatellite markers for Incarvillea sinensis (Bignoniaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Incarvillea sinensis var. sinensis (Bignoniaceae), an annual herb endemic to Inner Mongolia, to study the degree to which delayed self-fertilization is favored.
  
  
  RESULTS
  Eight polymorphic primer sets were isolated and characterized in two Inner Mongolia populations of I. sinensis var. sinensis with a relatively simple and fast subcloning method. Numbers of alleles per locus ranged from 2 to 7, with observed and expected heterozygosities ranging from 0 to 0.261 and from 0 to 0.778, respectively.
  
  
  CONCLUSIONS
  These markers will be useful for future studies of self-fertilization adaptability in I. sinensis var. sinensis.
authors.affiliation: State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany,Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Yu
authors.lastname: Jing-Gao
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Luo
authors.forename: Wei-Ning
authors.initials: WN
authors.lastname: Bai
mesh_terms: D000483:Alleles; D029402:Bignoniaceae / Q000235:genetics*; D017931:DNA Primers / Q000032:analysis* / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005787:Gene Frequency; D006580:Genetic Carrier Screening / Q000379:methods; D056426:Genetic Loci; D018745:Genome, Plant; D018895:Microsatellite Repeats; D059372:Nucleotide Motifs; D018515:Plant Leaves / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D057945:Self-Fertilization / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant