title: Chloroplast lipid droplet type II NAD(P)H quinone oxidoreductase is essential for prenylquinone metabolism and vitamin K1 accumulation.
abstract: Lipid droplets are ubiquitous cellular structures in eukaryotes and are required for lipid metabolism. Little is currently known about plant lipid droplets other than oil bodies. Here, we define dual roles for chloroplast lipid droplets (plastoglobules) in energy and prenylquinone metabolism. The prenylquinones--plastoquinone, plastochromanol-8, phylloquinone (vitamin K(1)), and tocopherol (vitamin E)--are partly stored in plastoglobules. This work shows that NAD(P)H dehydrogenase C1 (NDC1) (At5g08740), a type II NAD(P)H quinone oxidoreductase, associates with plastoglobules. NDC1 reduces a plastoquinone analog in vitro and affects the overall redox state of the total plastoquinone pool in vivo by reducing the plastoquinone reservoir of plastoglobules. Finally, NDC1 is required for normal plastochromanol-8 accumulation and is essential for vitamin K(1) production.
authors.affiliation: Laboratoire de Physiologie Végétale, Université de Neuchâtel, 2000 Neuchâtel, Switzerland.
authors.forename: Lucia
authors.initials: L
authors.lastname: Eugeni Piller
authors.forename: Céline
authors.initials: C
authors.lastname: Besagni
authors.forename: Brigitte
authors.initials: B
authors.lastname: Ksas
authors.forename: Dominique
authors.initials: D
authors.lastname: Rumeau
authors.forename: Claire
authors.initials: C
authors.lastname: Bréhélin
authors.forename: Gaétan
authors.initials: G
authors.lastname: Glauser
authors.forename: Felix
authors.initials: F
authors.lastname: Kessler
authors.forename: Michel
authors.initials: M
authors.lastname: Havaux
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D002839:Chromans / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D008055:Lipids / Q000737:chemistry*; D008163:Luminescent Measurements; D009154:Mutation / Q000235:genetics; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis; D010971:Plastoquinone / Q000378:metabolism*; D021381:Protein Transport; D011808:Quinone Reductases / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D024505:Tocopherols / Q000378:metabolism*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000378:metabolism; D010837:Vitamin K 1 / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D002839:Chromans; D008055:Lipids; D011809:Quinones; D014810:Vitamin E; C013649:plastochromanol 8; D010837:Vitamin K 1; D009247:NADH, NADPH Oxidoreductases; C561934:NDC1 protein, Arabidopsis; D011808:Quinone Reductases; D010971:Plastoquinone; D024505:Tocopherols