title: Conserved signaling through vascular endothelial growth (VEGF) receptor family members in murine lymphatic endothelial cells.
abstract: Lymphatic vessels guide interstitial fluid, modulate immune responses by regulating leukocyte and antigen trafficking to lymph nodes, and in a cancer setting enable tumor cells to track to regional lymph nodes. The aim of the study was to determine whether primary murine lymphatic endothelial cells (mLECs) show conserved vascular endothelial growth factor (VEGF) signaling pathways with human LECs (hLECs). LECs were successfully isolated from murine dermis and prostate. Similar to hLECs, vascular endothelial growth factor (VEGF) family ligands activated MAPK and pAkt intracellular signaling pathways in mLECs. We describe a robust protocol for isolation of mLECs which, by harnessing the power of transgenic and knockout mouse models, will be a useful tool to study how LEC phenotype contributes to alterations in lymphatic vessel formation and function.
authors.affiliation: Centre for Cancer Research, Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Coso
authors.forename: Yiping
authors.initials: Y
authors.lastname: Zeng
authors.forename: Dhanya
authors.initials: D
authors.lastname: Sooraj
authors.forename: Elizabeth D
authors.initials: ED
authors.lastname: Williams
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D008208:Lymphatic System / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D040262:Receptors, Vascular Endothelial Growth Factor / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D040262:Receptors, Vascular Endothelial Growth Factor