title: Mesoporous magnetic hollow nanoparticles-protein carriers for lysosome escaping and cytosolic delivery.
abstract: It is important for a controlled release system to determine whether nanoparticles can penetrate cell membranes and deliver protein into the nuclear or cytosolic compartments of cells, and thus function as carriers. Here, we prepared different functionalized mesoporous magnetic hollow nanoparticles (MMHs) and chose bovine serum albumin (BSA) as a model protein to detect the intracellular trafficking of MMHs. The results showed that MMHs modified with amino groups (AMMHs) were efficient in protein loading and that the loading was dependent on the pH, temperature and ionic strength. Furthermore, we found that the AMMHs not only transported BSA into the cells but also released the BSA carried into the nuclear or cytosolic compartments of the cells. In addition, the nanoparticles were biocompatible, and the encapsulation of BSA in AMMHs did not affect their bioactivity. Taken together, AMMHs are excellent carriers for releasing protein into the cytosol and nucleus, and they have the potential to be used in a controlled release system.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China. Graduate School of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Xinglu
authors.initials: X
authors.lastname: Huang
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Huiyu
authors.initials: H
authors.lastname: Liu
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Ren