title: Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer.
abstract: The role of thymidylate synthase (TS) is essential as a key rate-limiting enzyme in DNA synthesis. It is the primary target of fluorouracil and its derivates in colorectal cancer. In this study, TS mRNA expression was examined in primary tumor and normal tissues from 76 patients with high- risk stage II/III colorectal cancer by laser capture microdissection and polymerase chain reaction. Thirty (39.47%) patients were found to have higher TS expression in primary tumors with earlier stage (P = 0.018), lower histological grades (P = 0.001) and high frequency microsatellite instability (P = 0.000). Multivariate analysis showed that microsatellite instability, histological grade and number of lymph nodes examined are independent prognostic markers.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Xia
authors.forename: Yanzhao
authors.initials: Y
authors.lastname: Jia
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Guo
authors.forename: Bing
authors.initials: B
authors.lastname: Wei
authors.forename: Yawei
authors.initials: Y
authors.lastname: Hua
authors.forename: Shujun
authors.initials: S
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D003106:Colon / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D009367:Neoplasm Staging; D012007:Rectum / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: D013940:Thymidylate Synthase