title: Identification of Anaplasma spp. rickettsia isolated from horses from clinical disease cases in Poland.
abstract: This study was aimed at determining the cause of the diseases in five horses exhibiting symptoms of fever, joint oedema and ataxia and thrombocytopenia. The PCR technique revealed the presence in the blood of 16S RNA Anaplasma/Ehrlichia spp. genetic material. DNA amplification with primers EHR 521 and EHR 747 gave a product with a size of 247 bp. The sequence of the PCR product obtained showed a 97.6-99.6% similarity with a sequence of a fragment of 16S RNA Anaplasma phagocytophilum, gene number EU 090186 from GenBank. Intravenous administration of oxytetracycline at a dose of 8 mg/kg of body mass for 7 days resulted in a gradual recovery.
authors.affiliation: Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences Lublin, Lublin, Poland. ukaszek0@wp.pl
authors.forename: Ł
authors.initials: Ł
authors.lastname: Adaszek
authors.forename: S
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D000709:Anaplasma / Q000145:classification* / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D006734:Horse Diseases / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D006736:Horses; D010118:Oxytetracycline / Q000627:therapeutic use; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010118:Oxytetracycline