title: Superovulation strategies for 6 commonly used mouse strains.
abstract: We examined different weight ranges and hormone dosages to determine superovulation protocols for 6 mouse strains commonly used in genetic engineering: C57BL/6NHsd, B6(Cg)-Tyr(c-2J)/J, B6D2F1/Hsd, FVB/NHsd, BALB/cAnNCr, and Crl:CD1(ICR). Mice from each strain were divided into groups based on weight roughly corresponding to those of 3-, 4-, 5-, and 6-wk-old mice. Mice were treated with 5 IU pregnant mare serum gonadotropin (PMSG) and 5 IU human chorionic gonadotropin (HCG). The weights of mice that produced maximal numbers of oocytes in response to these doses were 14.2 g or less for C57BL/6NHsd, 13.7 g or less for B6(Cg)-Tyr(c-2J)/J, 6.0 to 9.9 g for B6D2F1/Hsd, 14.5 to 16.4 g for FVB/NHsd, 14.8 g or less for BALB/cAnNCr, and 23.5 g or more for Crl:CD1(ICR). We then compared PMSG dosages of 5 and 2.5 IU per mouse and determined whether 2 doses of PMSG (5 or 2.5 IU, depending on prior results) administered 1 wk apart, followed by the standard HCG injection, would produce more oocytes when compared to a single dose of PMSG. FVB, B6D2F1, BALB/c, and CD1 mice responded best to a single dose of 5 IU of each hormone, whereas B6(Cg)-Tyr (c-2J)/J mice produced more oocytes after 2.5 IU PMSG. Although C57BL/6 mice given the standard dose produced good numbers of oocytes, the number was higher after 2 doses of PMSG at 5 IU per dose. We conclude that response to superovulation can be optimized based on mouse strain, weight, and the dose and timing of hormone injection.
authors.affiliation: Department of Biochemistry and Molecular Biology, Genetically Engineered Mouse Facility, University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Charlie
authors.initials: C
authors.lastname: Luo
authors.forename: Juliana
authors.initials: J
authors.lastname: Zuñiga
authors.forename: Earnessa
authors.initials: E
authors.lastname: Edison
authors.forename: Shana
authors.initials: S
authors.lastname: Palla
authors.forename: Wenli
authors.initials: W
authors.lastname: Dong
authors.forename: Jan
authors.initials: J
authors.lastname: Parker-Thornburg
mesh_terms: D000367:Age Factors; D000818:Animals; D001835:Body Weight / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D011247:Pregnancy; D013045:Species Specificity; D013480:Superovulation / Q000187:drug effects* / Q000502:physiology*; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine