title: Oxidative stress induced apoptosis of human lung carcinoma (A549) cells by a novel copper nanorod formulation.
abstract: This study elucidates the process of synthesis of copper (Cu) nanorods using almond skin extract as stabilizing cum capping agent. These nanorods were (about 200 nm long and 40 nm wide) characterized by transmission electron microscopy (TEM). Further, cytotoxicity potential of these nanorods was evaluated in A549 cells (Human lung carcinoma cell line) via cell viability assay and extracellular lactate dehydrogenase (LDH) release. Also, reduced glutathione (GSH), lipid peroxidation (LPO), cellular oxidative stress (Rhodamine 123 florescence) and apoptosis (Annexin V FITC/Propidium iodide staining) were also investigated in control and treated cells. Results indicated that Cu nanorods induced apoptotic death of cancer cells by induction of oxidative stress, depletion of cellular antioxidants and mitochondrial dysfunction. This study reports a novel process of synthesis of almond skin extract capped Cu nanorods and its potential as an anticancer agent against A549 lung carcinoma cells.
authors.affiliation: Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India.
authors.forename: Menaka C
authors.initials: MC
authors.lastname: Thounaojam
authors.forename: Ravirajsinh N
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Mayur
authors.initials: M
authors.lastname: Valodkar
authors.forename: Padamanabhi S
authors.initials: PS
authors.lastname: Nagar
authors.forename: Ranjitsinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: Sonal
authors.initials: S
authors.lastname: Thakore
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002277:Carcinoma / Q000188:drug therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008175:Lung Neoplasms / Q000188:drug therapy*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008928:Mitochondria; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D003300:Copper