title: Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces.
abstract: We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly(ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino-terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.
authors.affiliation: Biophysical Engineering Group, MESA+ Institute for Nanotechnology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
authors.forename: Maryana
authors.initials: M
authors.lastname: Escalante
authors.forename: Pascale
authors.initials: P
authors.lastname: Maury
authors.forename: Christiaan M
authors.initials: CM
authors.lastname: Bruinink
authors.forename: Kees
authors.initials: K
authors.lastname: van der Werf
authors.forename: John D
authors.initials: JD
authors.lastname: Olsen
authors.forename: John A
authors.initials: JA
authors.lastname: Timney
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: C
authors.initials: C
authors.lastname: Neil Hunter
authors.forename: Vinod
authors.initials: V
authors.lastname: Subramaniam
authors.forename: Cees
authors.initials: C
authors.lastname: Otto