title: A ratiometric fluorescent probe for thiols based on a tetrakis(4-hydroxyphenyl)porphyrin-coumarin scaffold.
abstract: In this work, we have designed and synthesized the compound Ratio-HPSSC, based on a tetrakis(4-hydroxyphenyl)porphyrin-coumarin scaffold, as a new ratiometric fluorescent probe for thiols. The ratiometric probe Ratio-HPSSC is highly selective and sensitive to thiols. Importantly, the novel ratiometric probe exhibited a remarkable change in emission color from red to blue. This key feature allows Ratio-HPSSC to be employed for thiol detection by simple visual inspection. Furthermore, we have demonstrated that Ratio-HPSSC is suitable for ratiometric fluorescence imaging of thiols in living cells. We believe that the new ratiometric probe will find interesting applications in chemistry, biology, and medicine.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, People's Republic of China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Cao
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Yu
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D011874:Radiometry / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds