title: Close association of intestinal autofluorescence with the formation of severe oxidative damage in intestine of nematodes chronically exposed to Al(2)O(3)-nanoparticle.
abstract: In nematodes, acute exposure (24-h) to 8.1-30.6 mg/L Al(2)O(3)-nanoparticles (NPs) or Al(2)O(3) did not influence intestinal autofluorescence, whereas chronic exposure (10-d) to Al(2)O(3)-NPs at concentrations of 8.1-30.6 mg/L or Al(2)O(3) at concentrations of 23.1-30.6 mg/L induced significant increases of intestinal lipofuscin accumulation, and formation of severe stress response and oxidative damage in intestines. Moreover, significant differences of intestinal autofluorescence, stress response and oxidative damage in intestines of Al(2)O(3)-NPs exposed nematodes from those in Al(2)O(3) exposed nematodes were detected at examined concentrations. Oxidative damage in intestine was significantly correlated with intestinal autofluorescence in exposed nematodes, and oxidative damage in intestine was more closely associated with intestinal autofluorescence in nematodes exposed to Al(2)O(3)-NPs than exposed to Al(2)O(3). Thus, chronic exposure to Al(2)O(3)-NPs may cause adverse effects on intestinal lipofuscin accumulation by inducing the formation of more severe oxidative stress in intestines than exposure to Al(2)O(3) in nematodes.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
authors.forename: Shunhui
authors.initials: S
authors.lastname: Yu
authors.forename: Qi
authors.initials: Q
authors.lastname: Rui
authors.forename: Ting
authors.initials: T
authors.lastname: Cai
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Wu
authors.forename: Yinxia
authors.initials: Y
authors.lastname: Li
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D017173:Caenorhabditis elegans / Q000033:anatomy & histology* / Q000187:drug effects*; D005453:Fluorescence; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000473:pathology; D008062:Lipofuscin / Q000378:metabolism; D053768:Metal Nanoparticles / Q000009:adverse effects* / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D008062:Lipofuscin; D017382:Reactive Oxygen Species; D000537:Aluminum Oxide