title: Mitochondrial haplogroups associated with end-stage heart failure and coronary allograft vasculopathy in heart transplant patients.
abstract: OBJECTIVE
  Mitochondrial haplogroups are known to influence individual predisposition to a wide spectrum of metabolic and degenerative diseases, including ischaemic cardiovascular diseases. We have examined the influence of the mitochondrial DNA (mtDNA) background on the development of human end-stage heart failure (HF) in patients undergoing heart transplantation. The influence of mtDNA haplogroups on the incidence of transplant-related complications, mainly cardiac allograft vasculopathy (CAV), and on post-transplant survival was also studied.
  
  
  RESULTS
  The most common mitochondrial haplogroups in European populations were genotyped in 450 heart transplant recipients, 248 heart transplant donors, and 206 healthy controls. Mitochondrial haplogroups were determined by PCR amplification of short mtDNA fragments, followed by restriction fragment length polymorphism analysis. After adjustment for age and sex the frequency of haplogroup H was significantly higher in heart transplant recipients than in controls [OR: 1.86 (95% confidence intervals, CI: 1.27-2.74), P= 0.014], and in heart donors [OR: 1.47 (95% CI: 0.99-2.19), P= 0.032]. Likewise, haplogroup Uk was found significantly more frequently among CAV patients than in non-CAV heart allograft recipients [OR: 4.1 (95% CI: 1.51-11.42), P= 0.042]. Finally, heart donor haplogroups had no influence on the morbidity or mortality after heart transplantation.
  
  
  CONCLUSIONS
  Mitochondrial haplogroups behave like risk factors for the progress to end-stage HF in a Spanish cardiac transplant population. Mitochondrial DNA variants may have some influence on the appearance of cardiac transplant complications.
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas Alberto Sols CSIC-UAM, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.
authors.forename: M Esther
authors.initials: ME
authors.lastname: Gallardo
authors.forename: Pablo
authors.initials: P
authors.lastname: García-Pavía
authors.forename: Raquel
authors.initials: R
authors.lastname: Chamorro
authors.forename: María E
authors.initials: ME
authors.lastname: Vázquez
authors.forename: Manuel
authors.initials: M
authors.lastname: Gómez-Bueno
authors.forename: Isabel
authors.initials: I
authors.lastname: Millán
authors.forename: Berta
authors.initials: B
authors.lastname: Almoguera
authors.forename: Verónica
authors.initials: V
authors.lastname: Domingo
authors.forename: Javier
authors.initials: J
authors.lastname: Segovia
authors.forename: Carlos
authors.initials: C
authors.lastname: Vilches
authors.forename: Luis
authors.initials: L
authors.lastname: Alonso-Pulpón
authors.forename: Rafael
authors.initials: R
authors.lastname: Garesse
authors.forename: Belén
authors.initials: B
authors.lastname: Bornstein
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006239:Haplotypes / Q000235:genetics*; D006333:Heart Failure / Q000235:genetics* / Q000601:surgery; D016027:Heart Transplantation; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D011183:Postoperative Complications / Q000235:genetics; D011446:Prospective Studies; D014184:Transplantation, Homologous; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial