title: [Comparison of acute toxicity of extract of unprocessed indian atringbush root and its two different processed products].
abstract: OBJECTIVE
  To compare the acute toxicity and content of daphnoretin among extracts of unprocessed indian string-bush root and its two different processed products, and to provide a basis for discussion of the mechanism of two processed methods.
  
  
  METHODS
  Extracts of unprocessed indian stringbush root and processed indian stringbush root with "sweat" and "artificial sweat" were prepared. The mice were intragastrically administrated once with these three extracts, the mortalities of mice were observed, and the median lethal dose (LD50) of different extracts were calculated with Bliss method. The determination of daphnoretin in these three samples was performed by high performance liquid chromatography.
  
  
  RESULTS
  The LD50 of indian stringbush root extracts, indian stringbush root processed with "sweat" and with "artificial sweat" were 46.678, 72.190, 67.953 g x kg(-1), respectively. The contents of daphnoretin in unprocessed indian stringbush root, indian stringbush root processed with "sweat" and with "artificial sweat" were 0.189%, 0.407% and 0.345%, respectively.
  
  
  CONCLUSIONS
  The toxicity of indian stringbush root processed with both "sweat" and "artificial sweat" is lower than that of the original rude drug. But the decreasion of toxicity of processed products is not by the reduced daphoretin content.
authors.affiliation: Guiyang Medical College, Guiyang 550004, China.
authors.forename: Jinjuan
authors.initials: J
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guilin
authors.initials: G
authors.lastname: Zhang
authors.forename: Jianke
authors.initials: J
authors.lastname: Wang
authors.forename: Chang
authors.initials: C
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000737:chemistry / Q000633:toxicity; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000633:toxicity*; D018517:Plant Roots / Q000737:chemistry*; D018675:Toxicity Tests; D032365:Wikstroemia / Q000737:chemistry*
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C035316:daphnoretin