title: Supravital fluorometric apoptosis detection in a single mouse embryo using lab-on-a-chip.
abstract: Detection of apoptosis is one of the main criteria of preimplantation embryo growth potential assessment. Recent developments in lab-on-a-chip techniques has led to apoptosis detection and monitoring on a single cell or embryo level. However, single embryo apoptosis detection without a change in embryo developmental competence and post-examination "recovery" still remains a challenge. In this paper we present a lab-on-a-chip, co-working with miniaturized optical instrumentation, which allows supravital examination of single embryos for the presence of apoptotic blastomers with full after lab-on-a-chip study "recovery" and maintenance of their further developmental capacity.
authors.affiliation: Wrocław University of Technology, Faculty of Microsystem Electronics and Photonics, Janiszewski Str. 11/17, 50-372 Wrocław, Poland. rafal.walczak@pwr.wroc.pl
authors.forename: Rafał
authors.initials: R
authors.lastname: Walczak
authors.forename: Patrycja
authors.initials: P
authors.lastname: Śniadek
authors.forename: Jan A
authors.initials: JA
authors.lastname: Dziuban
authors.forename: Joanna
authors.initials: J
authors.lastname: Kluger
authors.forename: Anna Chełmońska
authors.initials: AC
authors.lastname: Soyta
mesh_terms: D000818:Animals; D017209:Apoptosis; D004622:Embryo, Mammalian / Q000166:cytology*; D005470:Fluorometry / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D051379:Mice; D008904:Miniaturization