title: α-Amylase monitoring by a novel amperometric biosensor based on Au electrode: its optimization, characterization, and application.
abstract: A low-cost and sensitive amperometric biosensor was developed for the determination of α-amylase activity. The biosensor was constructed by immobilizing glucose oxidase-gelatin via glutaraldehyde on the Au electrode surface. Measurements were carried out chronoamperometrically at -0.7 V. Several parameters such as glucose oxidase activity, gelatin amount, and glutaraldehyde percentage for cross-linking were optimized. Optimum pH, optimum temperature, repeatability, and storage stabilities of the biosensor were identified. Under the optimum experimental conditions, a linear calibration curve was obtained for α-amylase between 0.819 and 13.110 U/ml. Sample analyses were carried out by detecting α-amylase activities in baker's yeast samples.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Turkey. umut.mengulluoglu@ege.edu.tr
authors.forename: Umut
authors.initials: U
authors.lastname: Mengulluoglu
authors.forename: Cagri
authors.initials: C
authors.lastname: Altug
authors.forename: H Deniz
authors.initials: HD
authors.lastname: Ertugrul
authors.forename: Abdulkerim
authors.initials: A
authors.lastname: Yildiz
authors.forename: E Melis
authors.initials: EM
authors.lastname: Ekici
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinckaya
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D005976:Glutaral / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D016014:Linear Models; D010100:Oxygen / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D013499:Surface Properties; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D013213:Starch; D005949:Glucose Oxidase; D000516:alpha-Amylases; D005947:Glucose; D010100:Oxygen; D005976:Glutaral