title: [Evaluation of different methods in determination of low level HBsAg].
abstract: To evaluate chemiluminescent immunoassay (CLIA), electrochemiluminescent immunoassay (ECLIA) and ELISA in determination of low level HBsAg.
  
  
  
  According to the standard of CLIA Architect i2000, 70 samples were divided into three groups by HBsAg concentration : <1 ng/ml, 1-5 ng/ml and >4 ng/ml. The samples were also determined by ECLIA MODULAR<E>170 and ELISA, and the results were compared with those measured by CLIA Architect i2000.
  
  
  
  The concordance rates of ECLIA MODULAR<E>170 with Architect i2000 was 79.2% for <1 ng/ml group, 100% for 1-5 ng/ml group, 100% for >4 ng/ml group. And the concordance rates of ELISA with Architect i2000 was 0 % for <1 ng/ml group, 45.5% for 1-5 ng/ml group and 100 % for >4 ng/ml group.
  
  
  
  For determination of low level HBsAg,CLIA Architect i2000 and ECLIA MODULAR<E>170 have high credibility. ELISA is also credible when HBsAg >5 ng/ml, but not for low level HBsAg, especially for HBsAg <1 ng/ml.
authors.affiliation: Department of Clinical Laboratory, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China.
authors.forename: Chun-rong
authors.initials: CR
authors.lastname: Fei
authors.forename: Ai-qing
authors.initials: AQ
authors.lastname: Ye
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D006514:Hepatitis B Surface Antigens