title: Prevalence of Coxiella burnetii in ticks after a large outbreak of Q fever.
abstract: Q fever has emerged as an important human and veterinary public health problem in the Netherlands with major outbreaks in three consecutive years. Goat farms are probably the prime source from which Coxiella burnetii have spread throughout the environment, infecting people living in the vicinity. Coxiella burnetii infection not only spilled over from animal husbandry to humans but could also have spread to neighbouring wildlife and pets forming novel reservoirs and consequently posing another and lingering threat to humans, companion animals and livestock. In these cases, transmission routes other than airborne spread of contaminated aerosols may become significant. Therefore, the role of ticks in the transmission of Coxiella burnetii in the current situation was investigated. A total of 1891 questing Ixodes ricinus ticks and 1086 ticks feeding on pets, wildlife and livestock were tested by a recently developed multiplex Q-PCR. All ticks were negative, except for a few ticks feeding on a herd of recently vaccinated sheep. Coxiella-positive ticks were not detected after resampling this particular herd three months later. Based on these data we conclude that the current risk of acquiring Q fever from questing ticks in the Netherlands is negligible. However, for future risk assessments, it might be relevant to sample more ticks in the vicinity of previously C. burnetii infected goat farms and to assess whether C. burnetii can be transmitted transovarially and transstadially in I. ricinus ticks.
authors.affiliation: National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control (Cib), Laboratory for Zoonoses and Environmental Microbiology (LZO), Bilthoven, the Netherlands. Hein.Sprong@rivm.nl
authors.forename: H
authors.initials: H
authors.lastname: Sprong
authors.forename: E
authors.initials: E
authors.lastname: Tijsse-Klasen
authors.forename: M
authors.initials: M
authors.lastname: Langelaar
authors.forename: A
authors.initials: A
authors.lastname: De Bruin
authors.forename: M
authors.initials: M
authors.lastname: Fonville
authors.forename: F
authors.initials: F
authors.lastname: Gassner
authors.forename: W
authors.initials: W
authors.lastname: Takken
authors.forename: S
authors.initials: S
authors.lastname: Van Wieren
authors.forename: A
authors.initials: A
authors.lastname: Nijhof
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
authors.forename: C B M
authors.initials: CB
authors.lastname: Maassen
authors.forename: E-J
authors.initials: EJ
authors.lastname: Scholte
authors.forename: J W
authors.initials: JW
authors.lastname: Hovius
authors.forename: K
authors.initials: K
authors.lastname: Emil Hovius
authors.forename: E
authors.initials: E
authors.lastname: Spitalská
authors.forename: Y T
authors.initials: YT
authors.lastname: Van Duynhoven
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild; D002415:Cats; D002417:Cattle; D016997:Coxiella burnetii / Q000276:immunology* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D003670:Deer; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D015994:Incidence; D018884:Ixodes / Q000382:microbiology*; D009426:Netherlands / Q000453:epidemiology; D015995:Prevalence; D011634:Public Health; D011778:Q Fever / Q000453:epidemiology* / Q000382:microbiology; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000382:microbiology; D013984:Tick Infestations / Q000453:epidemiology* / Q000469:parasitology; D015047:Zoonoses
chemical_list: D004269:DNA, Bacterial