title: [Detection of specific IgG in the sera of patients with chronic schistosomiasis japonica by dot-ELISA with the recombinant Sj26-Sj32 fusion protein].
abstract: OBJECTIVE
  To study the diagnostic value of the Dot ELISA with rSj26-Sj32 fusion protein for chronic schistosomiasis japonica.
  
  
  METHODS
  rSj26-Sj32 fusion protein and SjAWA were used to establish the HRP-IgG-Dot-ELISA. Serum samples from patients with chronic schistosomiasis japonica (40 cases), clonorchiasis sinensis (21 cases), paragonimiasis westermani(13 cases), alveolar echinococcosis (10 cases), cystic echinococcosis(9 cases), hepatitis B(20 cases), pulmonary tuberculosis (20 cases) and healthy persons (43 cases) were examined.
  
  
  RESULTS
  Sensitivity and specificity were respectively 92.5% (37/40) and 95.4% (41/43) for rSj26-Sj32-Dot-ELISA and 95.0% (38/40) and 93.0% (40/43) for SjAWA-Dot-ELISA, and there was no significant difference between two antigens (P > 0.05). There were different cross reactions to the sera of patients with clonorchiasis sinensis, paragonimiasis westermani or alveolar echinococcosis, but no cross reaction to the sera of patients with cystic echinococcosis, hepatitis B or pulmonary tuberculosis. The positive and negative predictive value and efficiency of diagnosis of rSj26-Sj32-Dot-ELISA for chronic schistosomiasis japonica were 84.1% (37/44), 97.7% (129/132), and 94.3% (166/176), respectively, and those of SjAWA-Dot-ELISA were 77.6% (38/49), 98.4% (125/127), and 92.6% (163/176), respectively. There was no significant difference between the two methods (P > 0.05).
  
  
  CONCLUSIONS
  rSi26-Si32 fusion protein can be applied to immunodiagnosis for chronic schistosomiasis japonica.
authors.affiliation: Institute of Infections and Parasitic Diseases, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shi-fei
authors.initials: SF
authors.lastname: Cai
authors.forename: Wen-gui
authors.initials: WG
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D011237:Predictive Value of Tests; D011993:Recombinant Fusion Proteins; D012549:Schistosoma japonicum; D012554:Schistosomiasis japonica / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D012680:Sensitivity and Specificity; D044967:Serum
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins