title: Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic identification of cleavage sites.
abstract: Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based data-analysis resource termed WebPics (http://clipserve.clip.ubc.ca/pics/). We demonstrate the utility of WebPics analysis of PICS data by determining the substrate specificity of factor Xa from P6-P6', an important blood coagulation protease that proteolytically generates thrombin from prothrombin. PICS confirms existing data on non-prime site specificity and refines our knowledge of factor Xa prime-site selectivity.
authors.affiliation: Institute for Molecular Medicine and Cell Research, University of Freiburg, Germany. oliver.schilling@mol-med.uni-freiburg.de
authors.forename: Oliver
authors.initials: O
authors.lastname: Schilling
authors.forename: Ulrich
authors.initials: U
authors.lastname: auf dem Keller
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Overall
mesh_terms: D000595:Amino Acid Sequence; D015951:Factor Xa / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D020407:Internet; D019151:Peptide Library; D040901:Proteomics / Q000379:methods*; D012984:Software; D013379:Substrate Specificity
chemical_list: D019151:Peptide Library; D015951:Factor Xa