title: Analysis of plastid number, size, and distribution in Arabidopsis plants by light and fluorescence microscopy.
abstract: Methods are described which allow one to observe chloroplasts in mesophyll cells from leaves of Arabidopsis, determine their number per cell, measure their area, and determine a value for chloroplast coverage inside mesophyll cells. Non-green plastids can also be imaged either by using staining, or by exploiting fluorescent proteins targeted to the plastid in non-green parts of the plant, such as the roots, in transgenic Arabidopsis.
authors.affiliation: Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Leicestershire, UK. Kevin.Pyke@nottingham.ac.uk
authors.forename: Kevin
authors.initials: K
authors.lastname: Pyke
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D008027:Light; D058503:Mesophyll Cells / Q000166:cytology / Q000378:metabolism; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence / Q000379:methods*; D053141:Organelle Size; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D018087:Plastids / Q000378:metabolism*; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes