title: Crystallization and preliminary X-ray analysis of a cold-active β-galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4.
abstract: β-Galactosidases catalyze the hydrolysis of a galactosyl moiety from the nonreducing termini of oligosaccharides or from glycosides. A novel GH family 42 cold-active β-galactosidase identified from the psychrotrophic and halotolerant Planococcus sp. L4 (BgaP) was crystallized and a complete data set was collected from a single frozen crystal on an in-house X-ray source. The crystal diffracted to 2.8 Å resolution and belonged to space group P1, with unit-cell parameters a = 104.29, b = 118.12, c = 121.12 Å, α = 62.66, β = 69.48, γ = 70.74°. A likely Matthews coefficient of 2.58 Å(3) Da(-1) and solvent content of 52.32% suggested the presence of six protein subunits in the asymmetric unit.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Higher Education Mega Center, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.forename: Kui
authors.initials: K
authors.lastname: Wang
authors.forename: Zhongxing
authors.initials: Z
authors.lastname: Mo
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Hu
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D058642:Planococcus Bacteria / Q000201:enzymology*; D001616:beta-Galactosidase / Q000737:chemistry*
chemical_list: D001616:beta-Galactosidase