title: Kloeckera taiwanica sp. nov., an ascomycetous apiculate yeast species isolated from mushroom fruiting bodies.
abstract: Three apiculate yeast strains, EJ7M09(T), GJ5M15 and GJ15M04, isolated from mushrooms in Taiwan were found to represent a novel species of the genus Kloeckera. The phylogenetically closest relative of this novel species is Hanseniaspora occidentalis, but the type strain of H. occidentalis differed by 4.6 % divergence (25 substitutions; 5 gaps) in the sequence of the D1/D2 domain of the large subunit rRNA gene. This difference clearly suggests that the three strains represent a distinct species. As none of the strains that were examined in this study produced ascospores or exhibited conjugation on common sporulation medium either alone or in a pairwise mixture, this species could be considered as an anamorphic member of the genus Hanseniaspora, and a novel species, Kloeckera taiwanica sp. nov., is proposed, with EJ7M09(T) ( = BCRC 23182(T) = CBS 11434(T)) as the type strain.
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan, ROC.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan, ROC.
authors.forename: Li-Yin
authors.initials: LY
authors.lastname: Huang
authors.affiliation: Department of Hospitality Management, Chu Hwa University of Medical Technology, 91 Wenhua 1st Street, Zender, Tainan 717, Taiwan, ROC.
authors.forename: Shan-Fu
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan, ROC.
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D055322:Kloeckera / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D012336:RNA, Ribosomal, 16S