title: Crystallization and preliminary X-ray crystallographic studies of swine CD8α.
abstract: CD8αα homodimers or CD8αβ heterodimers form on the T-cell surface, where they are essential as co-receptors for MHC class I molecules in activation of the CTL response. To date, swine have been found to show the highest percentage of lymphocytes with surface expression of CD8α. Crystallographic analysis of swine CD8α (sCD8α) to 1.8 Å resolution revealed that the crystals belonged to space group P3(2)21, with unit-cell parameters a = 80.97, b = 80.97, c = 95.19 Å. The Matthews coefficient and the solvent content were calculated to be 3.23 Å(3) Da(-1) and 61.89%, respectively. These results may aid further structural and functional analyses of sCD8α.
authors.affiliation: Department of Microbiology and Immunology, College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Zhang
authors.forename: Jianxun
authors.initials: J
authors.lastname: Qi
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Pan
authors.forename: ZhaoSan
authors.initials: Z
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Chun
authors.initials: C
authors.lastname: Xia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016827:CD8 Antigens / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D016415:Sequence Alignment; D013552:Swine
chemical_list: D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain