title: Cloning and overexpression of transferrin gene from cypermethrin-resistant Culex pipiens pallens.
abstract: The complete sequence of transferrin has been cloned from cypermethrin-resistant strain of Culex pipiens pallens(Cr-C strain). Quantitative reverse transcription PCR analysis indicated that the transferrin transcription level was 12.6 times higher in Cr-C strain than in susceptible strain at fourth instar larvae. The transferrin expression was also found to be consistently higher throughout the life cycle of Cr-C strain. A protein of predicted size 90.8 kDa has been detected by Western blotting in transferrin-transfected mosquito C6/36 cells. These transferrin-transfected cells also showed enhanced cypermethrin resistance compared to null-transfected or plasmid vector-transfected cells as determined by methyl tritiated thymidine((3)H-TdR) incorporation. These results indicate that transferrin is expressed at higher levels in Cr-C strain and may confer some insecticide resistance in C. pipiens pallens.
authors.affiliation: Department of Pathogenic Biology, Jining Medical College, Jining, Shandong Province 272067, People's Republic of China. 1392144@163.com
authors.forename: Wenbin
authors.initials: W
authors.lastname: Tan
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.forename: Lijuan
authors.initials: L
authors.lastname: Liu
authors.forename: Haifang
authors.initials: H
authors.lastname: Wang
authors.forename: Maoqing
authors.initials: M
authors.lastname: Gong
authors.forename: Xin
authors.initials: X
authors.lastname: Quan
authors.forename: Honggang
authors.initials: H
authors.lastname: Gao
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects*; D015870:Gene Expression; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011722:Pyrethrins / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D014162:Transfection; D014168:Transferrin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D011722:Pyrethrins; D014168:Transferrin; C017160:cypermethrin