title: Transcriptome analysis of the effects of gomisin a on the recovery of carbon tetrachloride-induced damage in rat liver.
abstract: Gomisin A possesses a hepatic function-facilitating property in liver-injured rats. Its preventive action on carbon tetrachloride-induced cholestasis is due to maintenance of the function of the bile acids-independent fraction. To investigate alterations in gene expression after gomisin A treatment on injured rat liver, DNA microarray analyses were performed on a Rat 44K 4-Plex Gene Expression platform with duplicated reactions after gomisin A treatment. We identified 255 up-regulated and 230 down-regulated genes due to the effects of gomisin A on recovery of carbon tetrachloride-induced rat liver damage. For functional characterization of these genes, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes biochemical pathways analyses were performed. Many up-regulated or down-regulated genes were related to cell cycle or focal adhesion and cell death genes, respectively. Our microarray experiment indicated that the liver repair mechanism induced by gomisin A was strongly associated with increased gene expressions related to cell cycle and suppression of the gene expression related in cell death.
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resource & Life Science, Pusan National University, Miryang, Republic of Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Choi
authors.forename: In Soo
authors.initials: IS
authors.lastname: Choi
authors.forename: Sang Mong
authors.initials: SM
authors.lastname: Lee
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.forename: Young Whan
authors.initials: YW
authors.lastname: Choi
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Park