title: Cationic polyfluorenes for intracellular delivery of proteins.
abstract: Two cationic polyfluorene derivatives, quaternary amine 1 and guanidine 2 sheathed systems, were prepared as potential carriers to mediate import of proteins into cells without requiring covalent attachment to the protein. Neither polymer showed significant cytotoxicities (IC(50) 100 μM) when exposed to Clone 9 rat liver cells. Both polymers were shown to mediate import of a series of four proteins chosen because they have different pI values, sizes, and variable organic fluor attachments. Once inside the cells, the quaternary amine system 1 released more of its cargo into regions outside the lysosomes. In one exploratory experiment, pyrenebutyrate was shown to accelerate import of a protein system by polymer 1.
authors.affiliation: Department of Chemistry, Texas A&M University, Box 30012, College Station, TX 77841, USA.
authors.forename: Anyanee
authors.initials: A
authors.lastname: Kamkaew
authors.forename: Rola
authors.initials: R
authors.lastname: Barhoumi
authors.forename: Robert C
authors.initials: RC
authors.lastname: Burghardt
authors.forename: Kevin
authors.initials: K
authors.lastname: Burgess
mesh_terms: D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D005449:Fluorenes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D019791:Guanidine / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011506:Proteins / Q000737:chemistry*; D051381:Rats; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D000588:Amines; D002412:Cations; D005449:Fluorenes; D011108:Polymers; D011506:Proteins; D019791:Guanidine