title: Protein from red cabbage (Brassica oleracea) seeds with antifungal, antibacterial, and anticancer activities.
abstract: A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50=5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50=96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50=53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50=50 and 90 μM, respectively.
authors.affiliation: Institute of Plant Virology, Fujian Province Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, People's Republic of China. xiujuanye2004@gmail.com
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Ye
authors.forename: Tzi-Bun
authors.initials: TB
authors.lastname: Ng
authors.forename: Zu-Jian
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Lian-Hui
authors.initials: LH
authors.lastname: Xie
authors.forename: Evandro-Fei
authors.initials: EF
authors.lastname: Fang
authors.forename: Jack-Ho
authors.initials: JH
authors.lastname: Wong
authors.forename: Wen-Liang
authors.initials: WL
authors.lastname: Pan
authors.forename: Sze-Stephen-Cho
authors.initials: SS
authors.lastname: Wing
authors.forename: Yan-Bo
authors.initials: YB
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001937:Brassica / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005659:Fungicides, Industrial / Q000494:pharmacology; D006801:Humans; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012639:Seeds / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000972:Antineoplastic Agents, Phytogenic; D005659:Fungicides, Industrial; D010940:Plant Proteins