title: Convergent synthesis of homogeneous Glc1Man9GlcNAc2-protein and derivatives as ligands of molecular chaperones in protein quality control.
abstract: A detailed understanding of the molecular mechanism of chaperone-assisted protein quality control is often hampered by the lack of well-defined homogeneous glycoprotein probes. We describe here a highly convergent chemoenzymatic synthesis of the monoglucosylated glycoforms of bovine ribonuclease (RNase) as specific ligands of lectin-like chaperones calnexin (CNX) and calreticulin (CRT) that are known to recognize the monoglucosylated high-mannose oligosaccharide component of glycoproteins in protein folding. The synthesis of a selectively modified glycoform Gal(1)Glc(1)Man(9)GlcNAc(2)-RNase was accomplished by chemical synthesis of a large N-glycan oxazoline and its subsequent enzymatic ligation to GlcNAc-RNase under the catalysis of a glycosynthase. Selective removal of the terminal galactose by a β-galactosidase gave the Glc(1)Man(9)GlcNAc(2)-RNase glycoform in excellent yield. CD spectroscopic analysis and RNA-hydrolyzing assay indicated that the synthetic RNase glycoforms maintained essentially the same global conformations and were fully active as the natural bovine ribonuclease B. SPR binding studies revealed that the Glc(1)Man(9)GlcNAc(2)-RNase had high affinity to lectin CRT, while the synthetic Man(9)GlcNAc(2)-RNase glycoform and natural RNase B did not show CRT-binding activity. These results confirmed the essential role of the glucose moiety in the chaperone molecular recognition. Interestingly, the galactose-masked glycoform Gal(1)Glc(1)Man(9)GlcNAc(2)-RNase also showed significant affinity to lectin CRT, suggesting that a galactose β-1,4-linked to the key glucose moiety does not significantly block the lectin binding. These synthetic homogeneous glycoprotein probes should be valuable for a detailed mechanistic study on how molecular chaperones work in concert to distinguish between misfolded and folded glycoproteins in the protein quality control cycle.
authors.affiliation: Institute of Human Virology, Department of Biochemistry & Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
authors.forename: Mohammed N
authors.initials: MN
authors.lastname: Amin
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Rahman M
authors.initials: RM
authors.lastname: Mizanur
authors.forename: Lai-Xi
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000818:Animals; D037281:Calnexin / Q000737:chemistry; D037282:Calreticulin / Q000737:chemistry; D002417:Cattle; D008024:Ligands; D018832:Molecular Chaperones / Q000737:chemistry*; D015335:Molecular Probes / Q000138:chemical synthesis*; D012260:Ribonucleases / Q000138:chemical synthesis*; D000520:alpha-Glucosidases / Q000138:chemical synthesis*
chemical_list: D037282:Calreticulin; D008024:Ligands; D018832:Molecular Chaperones; D015335:Molecular Probes; D037281:Calnexin; D012260:Ribonucleases; D000520:alpha-Glucosidases