title: [Effect of separation and purification processes on extraction ratio of polysaccharides in Rabdosia rubescens and immunocompetence].
abstract: OBJECTIVE
  To select a good method for purifying polysaccharides from Rabdosia rubescens, and study their immunological activity.
  
  
  METHODS
  DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and stepwise ethanol precipitation were used to isolate and purify the polysaccharides from Rabdosia rubescens, respectively. MTT method was used to evaluate the immune activity.
  
  
  RESULTS
  Five components were obtained from DEAE-Sepharose Fast Flow (Cl-) weak polar anion-exchange chromatography and four polysaccharides were obtained through alcohol precipitation.
  
  
  CONCLUSIONS
  Polysaccharides from Rabdosia rubescens by stepwise ethanol precipitation show stronger immunological activity. Their immunomodulatory effect and related mechanism will be researched further.
authors.affiliation: College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Pei-Yi
authors.initials: PY
authors.lastname: Liu
authors.forename: Lan-Fang
authors.initials: LF
authors.lastname: Hao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D000431:Ethanol / Q000737:chemistry*; D037602:Isodon / Q000737:chemistry*; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015203:Reproducibility of Results; D012997:Solvents; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000276:Adjuvants, Immunologic; D011134:Polysaccharides; D012997:Solvents; D000431:Ethanol