title: Terpenoid metabolites from Spongia spp. and their effects on nucleic acid biosynthesis in sea urchin eggs.
abstract: 19-Norspongia-13(16),14-diene-3-one (1) was isolated for the first time from a natural source, along with a series of known spongiane diterpenoids (2-11) and sesquiterpene (12) from two unidentified species belonging to the genus Spongia. The effects of 1, 4, 5, 8-12 on biosynthesis of nucleic acids and embryonic development of the sea urchin Strongylocentrotus intermedius have been studied. All the compounds inhibit sea urchin embryo development at concentration of 20 microg/mL and above and DNA biosynthesis at the dose of 10 microg/mL. The inhibitory effect of diterpenoids at least partly may be explained by the inhibition of thymidine kinase activity.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, FEB RAS, pr. 100 let Vladivostoku, 159, Vladivostok 690022, Russian Federation. ponomarenko@piboc.dvo.ru
authors.forename: Ludmila P
authors.initials: LP
authors.lastname: Ponomarenko
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Terent'eva
authors.forename: Vladimir B
authors.initials: VB
authors.lastname: Krasokhin
authors.forename: Anatoliy I
authors.initials: AI
authors.lastname: Kalinovsky
authors.forename: Valery A
authors.initials: VA
authors.lastname: Rasskazov
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D015394:Molecular Structure; D010063:Ovum / Q000187:drug effects* / Q000502:physiology; D011161:Porifera / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D012617:Sea Urchins / Q000502:physiology*; D013729:Terpenes / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D013729:Terpenes; D012313:RNA; D004247:DNA