title: Identification of protein-protein interactions by surface plasmon resonance followed by mass spectrometry.
abstract: Elucidation of the function and meaning of the protein networks can be useful in the understanding of many pathological processes and the identification of new therapeutic targets. This unit describes an approach to discover protein-protein interactions by coupling surface plasmon resonance to mass spectrometry. Briefly, a protein is covalently bound to a sensor chip, which is then exposed to brain extracts injected over the surface via a microfluidic system. This allows the monitoring in real-time of the interactions between the immobilized ligand and the extracts. Interacting proteins from the extracts are then recovered, trypsinized, and identified using mass spectrometry. The data obtained are searched against a sequence database using the Mascot software. To exclude nonspecific interactors, control experiments using blank sensor chips, and/or randomized peptides, are performed. The protocol presented here does not require specific labeling or modification of proteins and can be performed in <4 days.
authors.affiliation: Karolinska Institute, Stockholm, Sweden.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Madeira
authors.forename: Elisabet
authors.initials: E
authors.lastname: Vikeved
authors.forename: Anna
authors.initials: A
authors.lastname: Nilsson
authors.forename: Benita
authors.initials: B
authors.lastname: Sjögren
authors.forename: Per E
authors.initials: PE
authors.lastname: Andrén
authors.forename: Per
authors.initials: P
authors.lastname: Svenningsson
mesh_terms: D013058:Mass Spectrometry / Q000379:methods*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D011506:Proteins