title: Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases.
abstract: BACKGROUND
  To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes. Our goal was to discover and characterize new glycoside hydrolases (GHases) from microbial biomass decay communities, especially those from unknown or never previously cultivated microorganisms.
  
  
  RESULTS
  From the metagenome sequences of an anaerobic microbial community actively decaying poplar biomass, we identified approximately 4,000 GHase homologs. Based on homology to GHase families/activities of interest and the quality of the sequences, candidates were selected for full-length cloning and subsequent expression. As an alternative strategy, a metagenome expression library was constructed and screened for GHase activities. These combined efforts resulted in the cloning of four novel GHases that could be successfully expressed in Escherichia coli. Further characterization showed that two enzymes showed significant activity on p-nitrophenyl-α-L-arabinofuranoside, one enzyme had significant activity against p-nitrophenyl-β-D-glucopyranoside, and one enzyme showed significant activity against p-nitrophenyl-β-D-xylopyranoside. Enzymes were also tested in the presence of ionic liquids.
  
  
  CONCLUSIONS
  Metagenomics provides a good resource for mining novel biomass degrading enzymes and for screening of cellulolytic enzyme activities. The four GHases that were cloned may have potential application for deconstruction of biomass pretreated with ionic liquids, as they remain active in the presence of up to 20% ionic liquid (except for 1-ethyl-3-methylimidazolium diethyl phosphate). Alternatively, ionic liquids might be used to immobilize or stabilize these enzymes for minimal solvent processing of biomass.
authors.affiliation: Brookhaven National Laboratory, Upton, NY, USA. vdlelied@rti.org.
authors.forename: Luen-Luen
authors.initials: LL
authors.lastname: Li
authors.forename: Safiyh
authors.initials: S
authors.lastname: Taghavi
authors.forename: Sean M
authors.initials: SM
authors.lastname: McCorkle
authors.forename: Yian-Biao
authors.initials: YB
authors.lastname: Zhang
authors.forename: Michael G
authors.initials: MG
authors.lastname: Blewitt
authors.forename: Roman
authors.initials: R
authors.lastname: Brunecky
authors.forename: William S
authors.initials: WS
authors.lastname: Adney
authors.forename: Michael E
authors.initials: ME
authors.lastname: Himmel
authors.forename: Phillip
authors.initials: P
authors.lastname: Brumm
authors.forename: Colleen
authors.initials: C
authors.lastname: Drinkwater
authors.forename: David A
authors.initials: DA
authors.lastname: Mead
authors.forename: Susannah G
authors.initials: SG
authors.lastname: Tringe
authors.forename: Daniel van der
authors.initials: Dv
authors.lastname: Lelie