title: Vascular endothelial growth factor (VEGF) and the VEGF soluble receptor-1 (sFlt-1) in chorionic villus tissue from Chinese women with early recurrent spontaneous abortion.
abstract: This case-control study explored the relationship between early recurrent spontaneous abortion (RSA) and the expression of two genes: VEGFA, the gene encoding vascular endothelial growth factor (VEGF); and fms-related tyrosine kinase 1 (FLT1), the gene encoding the soluble VEGF receptor-1 (sFlt-1). Women experiencing RSA or undergoing induced abortions in the early stage of normal pregnancy were recruited to the study (n = 30 per group). There were no significant between-group differences in maternal age or duration of pregnancy. The levels of VEGF and sFlt-1 mRNA in chorionic villus tissue samples were examined by quanti tative reverse transcription-polymerase chain reaction. Levels of sFlt-1 and VEGF mRNA in the chorionic villus tissue of women with RSA were significantly higher than levels in the control group. This study demonstrated that there is a relationship between early RSA and VEGF and sFlt-1 levels, suggesting that over-expression of the FLT1 and VEGFA genes may be associated with the pathogenesis of RSA.
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: L-H
authors.initials: LH
authors.lastname: Pang
authors.forename: M-J
authors.initials: MJ
authors.lastname: Li
authors.forename: M-Q
authors.initials: MQ
authors.lastname: Li
authors.forename: D-M
authors.initials: DM
authors.lastname: Yang
authors.forename: L
authors.initials: L
authors.lastname: Shi
mesh_terms: D000026:Abortion, Habitual / Q000378:metabolism*; D000022:Abortion, Spontaneous / Q000378:metabolism*; D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D002824:Chorionic Villi / Q000378:metabolism*; D017931:DNA Primers; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2