title: Prevention of intimal hyperplasia employing the cavitary two-layer method in allogeneic rat heart transplantation.
abstract: BACKGROUND
  The development of graft arteriosclerosis is a significant contributor to chronic rejection in organ transplant recipients. The purpose of the present study was to establish whether or not the cavitary two-layer method can prevent graft arteriosclerosis after rat heart transplantation.
  
  
  METHODS
  F-344 rats served as donors to Lewis recipients. Grafts in the control group (group C) were immediately allotransplanted without preservation. Grafts were also transplanted after cold preservation for 3 h in University of Wisconsin solution (group UW), or employing the cavitary two-layer (CTL) method (group TL). In another group (group W), grafts were subjected to 15-min warm ischemia and then transplanted. Grafts damaged by ischemia were also transplanted after preservation for 3 h using CTL (group WTL). We measured intimal thickening (IT) before transplantation and at 30 and 60 d post-transplant and also assessed the expression of heat shock proteins (HSPs).
  
  
  RESULTS
  At 60 d post-transplant, IT in group WTL was significantly lower than in group W (0.30 ± 0.03 versus 0.45 ± 0.04, respectively). In contrast, no significant changes were observed in the cold storage groups. Expression of HSPs 60 and 70 at 60 d in group WTL (25.40% ± 1.64% and 35.96% ± 2.65%, respectively) was reduced compared with group W (46.07% ± 5.84% and 55.11% ± 1.54%, respectively).
  
  
  CONCLUSIONS
  CTL reduces IT induced by warm ischemia in rat heart transplantation, and allows the maintenance of low HSP 60 and 70 expression.
authors.affiliation: Department of Surgery, Hyogo Cancer Center, Akashi, Japan. yasu120@hp.pref.hyogo.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Fujino
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Kamoda
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Tanioka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Sakai
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control*; D003080:Cold Temperature; D016027:Heart Transplantation / Q000379:methods*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D008297:Male; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous / Q000379:methods; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology*; D052096:Warm Ischemia / Q000379:methods
chemical_list: D006360:Heat-Shock Proteins