title: [Preparation and characterization of follwing the national standard anti-Brucella abortus serum, bovine].
abstract: To prepare anti-Brucella abortus serum used for calibrate the agglutination test follwing the national standard, 4 anti-Brucella abortus sera were obtained from 4 cows infected with Brucella abortus naturally. By potency testing, the third serum was selected. Sterility, vaccum degree, residual moisture, uniformity and stability of this standard material were tested and proved to meet the national standard. Referring to the international standard, RBT (Rose-Bengal plate agglutination test), SAT (standard tube agglutination) and CFT (complement fixation test) titers of this standard material were measured to be 1:160 "+" 1:2 400 "++" and 1:800 "++", which are identical with the collaborative assay results. International unit of the standard material is 4 000 IU/mL.
authors.affiliation: China Institute of Veterinary Drug Control, Beijing 100081, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Fushi
authors.initials: F
authors.lastname: Guan
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Dai
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.forename: Lianshou
authors.initials: L
authors.lastname: Lu
authors.forename: Zaishi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D002681:China; D003168:Complement Fixation Tests / Q000662:veterinary; D007106:Immune Sera; D012015:Reference Standards
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera