title: Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum.
abstract: Cellular composition of the adult zebrafish (Danio rerio) optic tectal cortex was examined in this study. Morphological techniques such as 1 μm thick serial plastic sections stained with osmium tetroxide and toluidine blue, modified rapid Golgi silver impregnation, GFAP immunohistochemistry, confocal microscopy, as well as scanning and transmission electron microscopy were used. Neuronal and glial components are described and the layers of the cortex are revisited. Specific neuronal arrangements as well as unique glial/ependymal cells are described. A three dimensional rendering of the astrocytic fiber arrangement in the marginal zone is presented and a composite drawing summarizes the cellular composition of the optic tectum.
authors.affiliation: Laboratory for Developmental Brain Research and Neuroplasticity, Department of Biological Sciences, Wagner College, Staten Island, New York 10301, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Corbo
authors.forename: Nidaa A
authors.initials: NA
authors.lastname: Othman
authors.forename: Michael C
authors.initials: MC
authors.lastname: Gutkin
authors.forename: Alejandra Del C
authors.initials: Adel C
authors.lastname: Alonso
authors.forename: Zoltan L
authors.initials: ZL
authors.lastname: Fulop
mesh_terms: D000818:Animals; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D013477:Superior Colliculi / Q000166:cytology*; D015027:Zebrafish / Q000033:anatomy & histology*