title: Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing Aspergillus niger.
abstract: Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Shinjuku, Tokyo, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Honda
authors.forename: Keiichi
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Kirimura
mesh_terms: D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D019343:Citric Acid / Q000378:metabolism*; D004265:DNA Helicases / Q000172:deficiency* / Q000235:genetics; D018390:Gene Targeting; D005800:Genes, Fungal; D005818:Genetic Engineering / Q000379:methods*; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D019343:Citric Acid; D004265:DNA Helicases