title: How nanoparticles encapsulating fluorophores allow a double detection of biomolecules by localized surface plasmon resonance and luminescence.
abstract: The paper shows how polysiloxane particles encapsulating fluorophores can be successfully used to detect biotin-streptavidin binding by two types of technique. After functionalization of the particles by streptavidin, the fixation of the biomolecule can indeed be detected by a shift of the localized surface plasmon resonance of the biotinylated gold dots used as substrate and by the luminescence of the fluorophores evidenced by scanning near-field optical microscopy. The development of particles allowing such a double detection opens a route for increasing the reliability of biological detection and for multi-labelling strategies crossing both detection principles.
authors.affiliation: Université de Lyon, Université Lyon 1, CNRS UMR 5620, Laboratoire de Physico-Chimie des Matériaux Luminescents (LPCML), Domaine Scientifique de La Doua, Bât Kastler, 10 rue André Marie Ampère 69622 Villeurbanne Cédex, France. Institut Charles Delaunay, CNRS FRE 2848, Laboratoire de Nanotechnologie et d'Instrumentation Optique (LNIO), Université de Technologie de Troyes, 12 rue Marie Curie BP 2060 10010 Troyes Cédex, France.
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authors.lastname: Descamps
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authors.lastname: Marquette
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authors.lastname: Jacquier