title: Growth of bifidobacteria and clostridia on human and cow milk saccharides.
abstract: For healthy infants, which were born normally and fully breastfed, the dominant component of the intestinal microflora are bifidobacteria. However, infants born by caesarean section possess clostridia as a dominant intestinal bacterial group. The aim of the present study was to determine whether bifidobacteria and clostridia are able to grow on human milk oligosaccharides (HMOs) and other carbon sources - lactose, cow milk (CM) and human milk (HM). Both bifidobacteria and clostridia grew on lactose and in CM. Bifidobacteria grew in HM and on HMOs. In contrast, 3 out of 5 strains of clostridia were not able to grow in HM. No clostridial strain was able to utilise HMOs. While both bifidobacterial strains were resistant to lysozyme, 4 out of 5 strains of clostridia were lysozyme-susceptible. It seems that HMOs together with lysozyme may act as prebiotic-bifidogenic compounds inhibiting intestinal clostridia.
authors.affiliation: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic.
authors.forename: Sarka
authors.initials: S
authors.lastname: Rockova
authors.forename: Vojtech
authors.initials: V
authors.lastname: Rada
authors.forename: Petr
authors.initials: P
authors.lastname: Marsik
authors.forename: Eva
authors.initials: E
authors.lastname: Vlkova
authors.forename: Vera
authors.initials: V
authors.lastname: Bunesova
authors.forename: Jan
authors.initials: J
authors.lastname: Sklenar
authors.forename: Igor
authors.initials: I
authors.lastname: Splichal
mesh_terms: D000818:Animals; D058491:Bacterial Load; D001644:Bifidobacterium / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D046972:Clostridium butyricum / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D003470:Culture Media; D005243:Feces / Q000382:microbiology; D006801:Humans; D007785:Lactose / Q000378:metabolism; D008892:Milk / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*; D009844:Oligosaccharides / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; D007785:Lactose