title: D-lactic acid interferes with the effects of platelet activating factor on bovine neutrophils.
abstract: D-lactic acidosis occurs in ruminants, such as cattle, with acute ruminal acidosis caused by ingestion of excessive amounts of highly fermentable carbohydrates. Affected animals show clinical signs similar to those of septic shock, as well as acute laminitis and liver abscesses. It has been proposed that the inflammatory response and susceptibility to infection could both be caused by the inhibition of phagocytic mechanisms. To determine the effects of d-lactic acid on bovine neutrophil functions, we pretreated cells with different concentrations of D-lactic acid and measured intracellular pH using 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and calcium flux using FLUO-3 AM-loaded neutrophils. Reactive oxygen species (ROS) production was measured using a luminol chemiluminescence assay, and MMP-9/gelatinase-B granule release was measured by zymography. CD11b and CD62L/l-selectin expression, changes in cell shape, superoxide anion production, phagocytosis of Escherichia coli-Texas red bioparticles, and apoptosis were all measured using flow cytometry. Our results demonstrated that D-lactic acid reduced ROS production, CD11b upregulation and MMP-9 release in bovine neutrophils treated with 100 nM platelet-activating factor (PAF). D-lactic acid induced MMP-9 release and, at higher concentrations, upregulated CD11b expression, decrease L-selectin expression, and induces late apoptosis. We concluded that D-lactic acid can interfere with neutrophil functions induced by PAF, leading to reduced innate immune responses during bacterial infections. Moreover, the increase of MMP-9 release and CD11b expression induced by 10mM D-lactic acid could promote an nonspecific neutrophil-dependent inflammatory reaction in cattle with acute ruminal acidosis.
authors.affiliation: Laboratory of Inflammation Pharmacology, Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Science, Universidad Austral de Chile, P.O. Box 567, Valdivia, Chile.
authors.forename: P
authors.initials: P
authors.lastname: Alarcón
authors.forename: I
authors.initials: I
authors.lastname: Conejeros
authors.forename: M D
authors.initials: MD
authors.lastname: Carretta
authors.forename: C
authors.initials: C
authors.lastname: Concha
authors.forename: E
authors.initials: E
authors.lastname: Jara
authors.forename: N
authors.initials: N
authors.lastname: Tadich
authors.forename: M A
authors.initials: MA
authors.lastname: Hidalgo
authors.forename: R A
authors.initials: RA
authors.lastname: Burgos
mesh_terms: D000140:Acidosis, Lactic / Q000378:metabolism / Q000662:veterinary; D000818:Animals; D039481:CD11b Antigen / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006863:Hydrogen-Ion Concentration; D019041:L-Selectin / Q000032:analysis; D019344:Lactic Acid / Q000494:pharmacology*; D008163:Luminescent Measurements / Q000662:veterinary; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000032:analysis
chemical_list: D039481:CD11b Antigen; D010972:Platelet Activating Factor; D017382:Reactive Oxygen Species; D019041:L-Selectin; D019344:Lactic Acid; D020780:Matrix Metalloproteinase 9; D002118:Calcium