title: BH3-only protein Bim inhibits activity of antiapoptotic members of Bcl-2 family when expressed in yeast.
abstract: Proteins of the Bcl-2 family regulate programmed cell death in mammals by promoting the release of cytochrome c from mitochondria in response to various proapoptotic stimuli. The mechanism by which BH3-only members of the family activate multidomain proapoptotic proteins Bax and Bak to form a pore in mitochondrial membranes remains under dispute. We report that cell death promoting activity of BH3-only protein Bim can be reconstituted in yeast when both Bax and antiapoptotic protein Bcl-X(L) are present, suggesting that Bim likely activates Bax indirectly by inhibiting antiapoptotic proteins.
authors.affiliation: Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.
authors.forename: Barbora
authors.initials: B
authors.lastname: Juhásová
authors.forename: Marek
authors.initials: M
authors.lastname: Mentel
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bhatia-Kiššová
authors.forename: Igor
authors.initials: I
authors.lastname: Zeman
authors.forename: Jordan
authors.initials: J
authors.lastname: Kolarov
authors.forename: Michael
authors.initials: M
authors.lastname: Forte
authors.forename: Peter
authors.initials: P
authors.lastname: Polčic
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism*; D000072224:Bcl-2-Like Protein 11; D002458:Cell Fractionation; D015151:Immunoblotting; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D051336:Mitochondrial Membranes / Q000378:metabolism; D010539:Permeability; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000235:genetics / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism*; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D000072224:Bcl-2-Like Protein 11; C000606776:Bcl2l11 protein, mouse; D008565:Membrane Proteins; D011518:Proto-Oncogene Proteins; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein