title: Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics.
abstract: A rapid and sensitive method for the determination of pinostrobin in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the first time. Isoliquiritigenin was used as an internal standard in rat plasma. Chromatographic separation was performed on an HiQ Sil C(18) column with isocratic elution at a flow rate of 1mL/min. The mobile phase consisted of water and methanol (9:91, v/v) containing 0.1% formic acid. The quantification limit was 10ng/mL within a linear range of 10-1000ng/mL (R=0.9984). The intra- and inter-day assay precision ranged from 3.8-5.3% to 3.2-5.2%, respectively, and the intra- and inter-day assay accuracy was between 93.2-95.1% and 95.5-104.3%, respectively. Our results indicated that the LC-MS/MS method is effective for pharmacokinetic study of pinostrobin in rat plasma.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
mesh_terms: D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D044950:Flavanones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000592:standards; D010276:Parasympatholytics / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000592:standards; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D044950:Flavanones; D010276:Parasympatholytics; C411294:pinostrobin