title: [Differential expression of colon cancer microRNA in microarry study].
abstract: OBJECTIVE
  To investigate the miRNA expression difference between colon cancer and normal colonic mucosa.
  
  
  METHODS
  Twelve (12) pairs of colorectal cancer tissue and normal colonic mucosa were collected, total RNA was extracted and purified. After fluorescent tags being added, hybridization was carried out on miRNA microarray chip (Affymetrix company). SAM analysis was performed to find out the significant expression difference, then the difference was verified by RT-PCR, finally target gene analysis software was utilized to predict the miRNA function.
  
  
  RESULTS
  The up-regulated miRNAs in colon cancer included has-miR-182, has-miR-17, hasmiR-106a, has-miR-93, has-miR-200c, has-miR-92a, has-let-7a, has-miR-20a (FDR value < 5%), while the downregulated miRNAs were has-miR-l195, has-miR-143, has-miR-145 (FDR value < 5%).
  
  
  CONCLUSIONS
  There is significant difference of miRNA expression between colon caner and normal colonic mucosa.
authors.affiliation: Department of Gastrointestinal Surgery , West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Ma
authors.forename: Lie
authors.initials: L
authors.lastname: Yang
authors.forename: Cun
authors.initials: C
authors.lastname: Wang
authors.forename: Yong-Yang
authors.initials: YY
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Zhong-Guang
authors.initials: ZG
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D046228:Microarray Analysis; D008875:Middle Aged
chemical_list: D035683:MicroRNAs