title: Keratinase Production by Three Bacillus spp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation.
abstract: Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62-75%) and feather (40-95%) producing 3.9-4.4 mg/ml of soluble protein in feather meal medium and 1.9-3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPPG), Bloco I, Centro de Ciências da Saúde (CCS), Universidade Federal do Rio de Janeiro (UFRJ), Cidade Universitária, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brazil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Mazotto
authors.forename: Rosalie Reed Rodrigues
authors.initials: RR
authors.lastname: Coelho
authors.forename: Sabrina Martins Lage
authors.initials: SM
authors.lastname: Cedrola
authors.forename: Marcos Fábio
authors.initials: MF
authors.lastname: de Lima
authors.forename: Sonia
authors.initials: S
authors.lastname: Couri
authors.forename: Edilma
authors.initials: E
authors.lastname: Paraguai de Souza
authors.forename: Alane Beatriz
authors.initials: AB
authors.lastname: Vermelho