title: Flavonoid profiling in three citrus varieties native to the Republic of Korea using liquid chromatography coupled with tandem mass spectrometry: contribution to overall antioxidant activity.
abstract: A mixture of flavonoid components was isolated from the fruit peel of three varieties of citrus native to Republic of Korea, Citrus leiocarpa Hort. ex Tanaka (CLHT), Citrus aurantium L. (CAL) and Citrus erythrosa Hort. (CEH), via 70% methanol extraction followed by ethyl acetate elution over a silica gel cartridge. The flavonoid components of the mixture were analyzed via high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in positive-ion mode and a comparison of the reported data. Among 17 characterized components, two flavanones, four flavones and two coumarin derivatives in the fruit peel of the three varieties were identified for the first time. The individual characterized components were quantified via HPLC-UV. The flavanones dominated in CAL, whereas the flavones prevailed in CLHT and CEH. The antioxidant activity of the flavonoid mixture of the fruit peel was determined via DPPH•, ABTS•⁺ and reducing power assays. The antioxidant activity of CEH and CAL was greater than that of CLHT.
authors.affiliation: Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Hae Gyeong
authors.initials: HG
authors.lastname: Kim
authors.forename: Gon-Sup
authors.initials: GS
authors.lastname: Kim
authors.forename: Semin
authors.initials: S
authors.lastname: Park
authors.forename: Jung Han
authors.initials: JH
authors.lastname: Lee
authors.forename: On Nuri
authors.initials: ON
authors.lastname: Seo
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Jin
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Shin
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002957:Citrus / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D056910:Republic of Korea; D013451:Sulfonic Acids / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D010851:Picrates; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl