title: Preparation of methacrylate-based monolith for capillary hydrophilic interaction chromatography and its application in determination of nucleosides in urine.
abstract: A novel poly(N-acryloyltris(hydroxymethyl)aminomethane-co-pentaerythritol triacrylate) (NAHAM-co-PETA) monolith was prepared in the 100 μm i.d. capillary and investigated for capillary liquid chromatography (cLC). The polymer monolith was synthesized by in situ polymerization of NAHAM and PETA in the presence of polyethylene glycol (PEG) in dimethyl sulfoxide (DMSO) as the porogen. The porous structure of monolith was optimized by changing the ratio of NAHAM to PETA, the molecular weight and amount of PEG. To evaluate the separation performance of the resultant polymer monolith, several groups of model compounds (including nucleosides, benzoic acids and anilines) were selected to perform cLC separation. Our results showed that these model compounds can be baseline separated on the resultant poly(NAHAM-co-PETA) monolithic column with the optimized mobile phases. The column efficiency was estimated to be 87,000 plates/m for acrylamide. In addition, this monolithic column was coupled with on-line solid-phase microextraction (SPME) for the analysis of four nucleosides (uridine, adenosine, cytidine, guanosine) in urine. The limit of detection of the proposed method was in the range from 40 to 52 ng/mL. The method reproducibility was obtained by evaluating the intra- and inter-day precisions with relative standard deviations (RSDs) less than 8.3% and 10.2%, respectively. Recoveries of the target analytes from spiked urine samples were ranged from 86.5% to 106.8%.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Ming-Luan
authors.initials: ML
authors.lastname: Chen
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wei
authors.forename: Bi-Feng
authors.initials: BF
authors.lastname: Yuan
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000814:Aniline Compounds / Q000737:chemistry / Q000302:isolation & purification; D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D017743:Equipment Reuse; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D016014:Linear Models; D008956:Models, Chemical; D009369:Neoplasms; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D011092:Polyethylene Glycols; D016062:Porosity; D011409:Propylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction
chemical_list: D000179:Acrylates; D000814:Aniline Compounds; D001565:Benzoates; D009705:Nucleosides; D011409:Propylene Glycols; C512194:poly(N-acryloyltris(hydroxymethyl)aminomethane); D011092:Polyethylene Glycols; C027992:pentaerythrityl triacrylate