title: Resistance training restores the gene expression of molecules related to fat oxidation and lipogenesis in the liver of ovariectomized rats.
abstract: Decreased levels of estrogen are associated with hepatic steatosis (HS), through changes in gene expression of molecules related to fat oxidation and lipogenesis. Both resistance training (RT) and endurance training (ET) prevent HS in ovariectomized (Ovx) rats. However, the molecular events associated with this process were only investigated for ET, but not for RT. Thus, the aim of this study was to investigate the effects of Ovx and RT on the gene expression of molecules related to fat oxidation and lipogenesis in the liver of rats. Sprague-Dawley adult female rats were grouped into four (n = 6 per group): sham-operated sedentary (Sham-Sed); Ovx sedentary (Ovx-Sed); sham-Rt and Ovx-Rt. A 10-week RT period, during which the animals climbed a 1.1-m vertical ladder with weights attached to their tails, was used. The sessions were performed three times a week, with 4-9 climbs and 8-12 dynamic movements per climb. Gene expression was analyzed by RT-PCR by the ∆∆Ct method. The estrogen deficiency associated with ovariectomy decreased the gene expression of molecules related to fat oxidation, carnitine palmitoyltransferase I (53%) and β-hydroxyacyl-CoA dehydrogenase (27%), and increased molecules related to lipogenesis, sterol regulatory element-binding protein-1c (106%), acetyl-CoA carboxylase (ACC) (72%) and stearoyl CoA desaturase-1 (109%). With the exception of ACC, the ovariectomy-induced changes in the expression of these molecules were restored by RT. The present results indicate that RT has important effects on the prevention of HS in Ovx animals, through changes in gene expression of molecules related to hepatic lipid metabolism.
authors.affiliation: Department of Physiological Sciences, Federal University of São Carlos, Flauzino Marques St, n. 67, ap: 14b, São Carlos, São Paulo 13562-002, Brazil. mmoraesdomingos@gmail.com
authors.forename: Mateus Moraes
authors.initials: MM
authors.lastname: Domingos
authors.forename: Maria Fernanda Cury
authors.initials: MF
authors.lastname: Rodrigues
authors.forename: Uliana Sbeguen
authors.initials: US
authors.lastname: Stotzer
authors.forename: Danilo Rodrigues
authors.initials: DR
authors.lastname: Bertucci
authors.forename: Markus Vinicius Campos
authors.initials: MV
authors.lastname: Souza
authors.forename: Diego Adorna
authors.initials: DA
authors.lastname: Marine
authors.forename: Camila do Valle Gomes
authors.initials: Cdo V
authors.lastname: Gatto
authors.forename: Heloisa Sobreiro Selistre
authors.initials: HS
authors.lastname: de Araújo
authors.forename: Sérgio Eduardo
authors.initials: SE
authors.lastname: de Andrade Perez
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics / Q000378:metabolism; D004967:Estrogens / Q000172:deficiency; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D005260:Female; D005786:Gene Expression Regulation; D050356:Lipid Metabolism / Q000235:genetics; D050155:Lipogenesis / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000378:metabolism*; D010052:Ovariectomy; D010084:Oxidation-Reduction; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D055070:Resistance Training; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
chemical_list: D004967:Estrogens; D047493:PPAR alpha; C497389:Srebf1 protein, rat; D051780:Sterol Regulatory Element Binding Protein 1; D015094:3-Hydroxyacyl CoA Dehydrogenases; D013230:Stearoyl-CoA Desaturase; D002334:Carnitine O-Palmitoyltransferase; D000103:Acetyl-CoA Carboxylase