title: [Inhibitive effect of C-21 steroidal glycosides of Cynanchum auriculatum on rat glioma cells in vitro].
abstract: To evaluate the inhibitive effect of C-21 steroidal glycosides from the root of Cynanchum auriculatum (CGB) on rat glioma C6 cells.
  
  
  
  C6 cells were treated with CGB for 24, 48,72 h at concentration of 30, 60, 120 mg/L, respectively. MTT assay was used for evaluating cell viability; fluorescence-activated cell sorting analysis after Annexin V/propidium iodide staining or single propidium iodide staining was used to test cell apoptosis and cell cycle.
  
  
  
  CGB at 30, 60, 120 mg/L concentration-dependently decreased C6 cell viability (P<0.001). CGB at 60 and 120 mg/L induced C6 cell apoptosis and cell cycle arrest. The fraction of G0/G1 cells was increased (P<0.05) and that of S phase cells was decreased (P<0.01).
  
  
  
  CGB can inhibit the growth of rat glioma C6 cells, and induce apoptosis and G0/G1 cell cycle arrest.
authors.affiliation: College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Yi-qi
authors.initials: YQ
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Ru-song
authors.initials: RS
authors.lastname: Zhang
authors.forename: Er-qing
authors.initials: EQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D031105:Cynanchum / Q000737:chemistry*; D005910:Glioma / Q000473:pathology*; D006027:Glycosides; D009005:Monosaccharides / Q000494:pharmacology*; D051381:Rats; D013256:Steroids / Q000494:pharmacology*
chemical_list: C438934:C-glycoside; D006027:Glycosides; D009005:Monosaccharides; D013256:Steroids