title: Insights into [FeFe]-hydrogenase structure, mechanism, and maturation.
abstract: Hydrogenases are metalloenzymes that are key to energy metabolism in a variety of microbial communities. Divided into three classes based on their metal content, the [Fe]-, [FeFe]-, and [NiFe]-hydrogenases are evolutionarily unrelated but share similar nonprotein ligand assemblies at their active site metal centers that are not observed elsewhere in biology. These nonprotein ligands are critical in tuning enzyme reactivity, and their synthesis and incorporation into the active site clusters require a number of specific maturation enzymes. The wealth of structural information on different classes and different states of hydrogenase enzymes, biosynthetic intermediates, and maturation enzymes has contributed significantly to understanding the biochemistry of hydrogen metabolism. This review highlights the unique structural features of hydrogenases and emphasizes the recent biochemical and structural work that has created a clearer picture of the [FeFe]-hydrogenase maturation pathway.
authors.affiliation: Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Montana State University, Bozeman, MT 59717, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Mulder
authors.forename: Eric M
authors.initials: EM
authors.lastname: Shepard
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Meuser
authors.forename: Neelambari
authors.initials: N
authors.lastname: Joshi
authors.forename: Paul W
authors.initials: PW
authors.lastname: King
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Posewitz
authors.forename: Joan B
authors.initials: JB
authors.lastname: Broderick
authors.forename: John W
authors.initials: JW
authors.lastname: Peters
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D020134:Catalytic Domain; D006864:Hydrogenase / Q000096:biosynthesis / Q000737:chemistry*; D008958:Models, Molecular; D019159:Nonheme Iron Proteins / Q000096:biosynthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D011499:Protein Processing, Post-Translational; D017433:Protein Structure, Secondary
chemical_list: D001426:Bacterial Proteins; D019159:Nonheme Iron Proteins; D006864:Hydrogenase