title: Molecular characterization and regulation of the angiotensin-converting enzyme type 2/angiotensin-(1-7)/MAS receptor axis during the ovulation process in cattle.
abstract: The objective of this study was to characterize the profiles of Ang-(1-7), MAS receptor, ACE(2), NEP and PEP during the ovulatory process in cattle. For this study, 40 synchronized cows with follicular diameter ≥ 12 mm were ovariectomized at different time-points (0, 3, 6, 12 and 24 h) after i.m. application of gonadotropin-releasing hormone (GnRH) to induce a luteinizing hormone surge. Follicular fluid was collected for measuring Ang-(1-7) by radioimmunoassay. Theca and granulosa cells were isolated from the preovulatory follicles to evaluate the gene expression of MAS receptor, ACE(2), NEP and PEP by qRT-PCR assay. Cross-contamination between theca and granulosa cells was tested by RT-PCR to detect cytochrome P450 aromatase (CYP19A1) and 17α-hydroxylase (CYP17A1) mRNA. Ang-(1-7) levels were constant until 12 h and then increased (p < 0.05) at 24 h after GnRH. Messenger RNA expression of MAS, ACE(2), NEP and PEP was detected in theca and granulosa cells at all time-points after GnRH. In granulosa cells, ACE(2), NEP and PEP were differentially expressed after GnRH treatment (p < 0.05). In conclusion, the Ang-(1-7), MAS receptor, ACE(2), NEP and PEP profiles in preovulatory follicles indicate that Ang-(1-7) plays a role in the regulation of the ovulatory process in cattle.
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction-BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Joabel
authors.initials: J
authors.lastname: Tonellotto dos Santos
authors.forename: Rogério
authors.initials: R
authors.lastname: Ferreira
authors.forename: Bernardo Garziera
authors.initials: BG
authors.lastname: Gasperin
authors.forename: Lucas Carvalho
authors.initials: LC
authors.lastname: Siqueira
authors.forename: João Francisco
authors.initials: JF
authors.lastname: de Oliveira
authors.forename: Robson A S
authors.initials: RA
authors.lastname: Santos
authors.forename: Adelina M
authors.initials: AM
authors.lastname: Reis
authors.forename: Paulo Bayard
authors.initials: PB
authors.lastname: Gonçalves
mesh_terms: D000803:Angiotensin I / Q000235:genetics* / Q000378:metabolism; D000085962:Angiotensin-Converting Enzyme 2; D000818:Animals; D002417:Cattle; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D005786:Gene Expression Regulation; D006107:Granulosa Cells / Q000378:metabolism; D023421:Models, Animal; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism; D010052:Ovariectomy; D010060:Ovulation / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000235:genetics* / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013799:Theca Cells / Q000378:metabolism
chemical_list: D010446:Peptide Fragments; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A; D000085962:Angiotensin-Converting Enzyme 2; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases; D015260:Neprilysin; C118790:angiotensin I (1-7)