title: Early distribution of intravenously injected mesenchymal stem cells in rats with acute brain trauma evaluated by (99m)Tc-HMPAO labeling.
abstract: BACKGROUND
  Stem cell tracking is essential for evaluation of its migration, transplantation and therapeutic response. The aim of this study was to evaluate early distribution of intravenously transplanted rat bone marrow mesenchymal stem cells (BMSCs) in rats with acute cerebral trauma by labeling with (99m)Tc-hexamethylpropyleneamine oxime ((99m)Tc-HMPAO).
  
  
  METHODS
  (99m)Tc-HMPAO-labeled BMSCs were injected intravenously to trauma rats (n=14) and sham-operated controls (n=13). Gamma camera images were acquired at 4 h after injection, and then organs were removed for gamma counting. Confocal microscope was used to confirm the migration of (99m)Tc-BMSCs by co-labeling with PKH26. Cytometric analysis was performed to evaluate apoptotic or necrotic change until the seventh day after labeling.
  
  
  RESULTS
  (99m)Tc-BMSCs were distributed mostly to lungs, liver and spleen at 4 h, and uptake of these organs was not significantly different between traumatic rats and controls. Meanwhile, the cerebral uptake of (99m)Tc-BMSCs was significantly higher in the traumatic rats than in controls (0.40% vs. 0.20%; P=.0002). Additionally, (99m)Tc-BMSCs' uptake of traumatic hemisphere was significantly higher than that of contralateral ones (0.27% vs. 0.13%; P=.0001) in traumatic rats. Regardless of radiolabeling, BMSCs migrated to traumatic regions, but not to nontraumatic hemispheres. However, gamma camera failed to demonstrate (99m)Tc-BMSCs in traumatic hemispheres. No significant apoptotic or necrotic change was observed until 7 days after radiolabeling.
  
  
  CONCLUSIONS
  Early distribution of BMSCs in traumatic brain disease could be monitored by (99m)Tc-labeling, which does not induce cellular death. However, our data showed that the amount of migrated (99m)Tc-BMSCs was not enough to be demonstrated by clinical gamma camera.
authors.affiliation: Department of Nuclear Medicine and Molecular Imaging, Ajou University School of Medicine, Suwon, Republic of Korea.
authors.forename: Bok-Nam
authors.initials: BN
authors.lastname: Park
authors.forename: Wooyoung
authors.initials: W
authors.lastname: Shim
authors.forename: Gwang
authors.initials: G
authors.lastname: Lee
authors.forename: Oh Young
authors.initials: OY
authors.lastname: Bang
authors.forename: Young-Sil
authors.initials: YS
authors.lastname: An
authors.forename: Joon-Kee
authors.initials: JK
authors.lastname: Yoon
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Ahn
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000000981:diagnostic imaging*; D016022:Case-Control Studies; D005260:Female; D005456:Fluorescent Dyes; D015902:Gamma Cameras; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008859:Microscopy, Polarization; D009336:Necrosis / Q000139:chemically induced; D009930:Organic Chemicals; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D019690:Technetium Tc 99m Exametazime / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime