title: [Anti-tumor activity of components isolated from purple sweet potato polysaccharides].
abstract: To isolate and purify components from polysaccharides of purple sweet potato (PPSP) and to test their anti-tumor activity.
  
  
  
  DEAE-Cellulose and CM-Cellulose exchange chromatography were applied to separate components of PPSP. The anti-tumor activities of each component were measured by MTT assay on Hela and HepG(2) cells and their monosaccharide composition were analyzed by TLC chromatography, followed by infrared spectroscopy studies.
  
  
  
  Through weak anion exchange chromatography and gradient elution by sodium chloride solution, four components were separated and named as PPSP, PPSPII, PPSPIII and PPSPIV, respectively. MTT tests showed that PPSP II and PPSPIII inhibited Hela and HepG2 tumor cells in a certain extent. The structural analysis revealed that PPSPI was mainly composed of glucose and galactose, PPSP II was composed of glucose and had a typical absorption peak of β-D-glucose chitosan pyranose, PPSP III was a glycoprotein showing a protein absorption peak.
  
  
  
  Four components were separated from PPSP successfully, among which PPSP II and PPSP III shows anti-tumor activities on Hela and HepG(2) cells in vitro.
authors.affiliation: School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Ruan
authors.forename: Qiu-ping
authors.initials: QP
authors.lastname: Gao
authors.forename: Meng-ya
authors.initials: MY
authors.lastname: Li
authors.forename: Ye-qi
authors.initials: YQ
authors.lastname: Tao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D027723:Ipomoea batatas / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D011134:Polysaccharides