title: The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators.
abstract: Covalent attachment of small ubiquitin-like modifier (SUMO) to proteins regulates many processes in the eukaryotic cell. This reaction is similar to ubiquitination and usually requires an E3 ligase for substrate modification. However, only a few SUMO ligases have been described so far, which frequently facilitate sumoylation by bringing together the SUMO-conjugating enzyme Ubc9 and the target protein. Ubc9 is an interaction partner of the transcription factor Krox20, a key regulator of hindbrain development. Here, we show that Krox20 functions as a SUMO ligase for its coregulators--the Nab proteins--and that Nab sumoylation negatively modulates Krox20 transcriptional activity in vivo.
authors.affiliation: Stem Cells, CABIMER (CSIC), Avenida Americo Vespucio, Seville E-41092, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: García-Gutiérrez
authors.forename: Francisco
authors.initials: F
authors.lastname: Juárez-Vicente
authors.forename: Francisco
authors.initials: F
authors.lastname: Gallardo-Chamizo
authors.forename: Patrick
authors.initials: P
authors.lastname: Charnay
authors.forename: Mario
authors.initials: M
authors.lastname: García-Domínguez
mesh_terms: D002460:Cell Line; D002843:Chromatin / Q000378:metabolism; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism*; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism; D058207:Sumoylation; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D002843:Chromatin; D051767:Early Growth Response Protein 2; C491000:NAB2 protein, human; D012097:Repressor Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D000098843:Ubiquitin-Conjugating Enzyme UBC9