title: Chemical assistance in refolding of bacterial inclusion bodies.
abstract: Escherichia coli is one of the most widely used hosts for the production of recombinant proteins but insoluble expression of heterologous proteins is a major bottleneck in production of recombinant proteins in E. coli. In vitro refolding of inclusion body into proteins with native conformations is a solution for this problem but there is a need for optimization of condition for each protein specifically. Several approaches have been described for in vitro refolding; most of them involve the use of additives for assisting correct folding. Cosolutes play a major role in refolding process and can be classified according to their function as aggregation suppressors and folding enhancers. This paper presents a review of additives that are used in refolding process of insoluble recombinant proteins in small scale and industrial processes.
authors.affiliation: Medical Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13169 43551, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Alibolandi
authors.forename: Hasan
authors.initials: H
authors.lastname: Mirzahoseini