title: [In vitro study on the anti-HSV-1 and HBV activities of extracts from the fruit of Eucalyptus maidenii].
abstract: OBJECTIVE
  To investigate the antiviral activities of three kinds of extracts from the fruit of Eucalyptus maidenii against herpes simplex virus typel and Hepatitis B Virus.
  
  
  METHODS
  Cytotoxicity of extracts on Vero cell lines were estimated using MTT method and anti-HSV-1 activity was observed and determined with CPE and plaque reduction assay. The inhibitory effects of extracts on HBsAg and HBeAg secretion in HepG2.2.15 cell culture were detected using ELISA.
  
  
  RESULTS
  Aqueous extract (pl8-E3) had conspicuous anti-HSV-1 activity, the IC50 was 126.77 microg/mL,but the EtOAc extracts( pl8-E1 )and MeOH extracts (pl8-E2) showed little anti-HSV-1 activity. None of these extracts had significant inhibitory eflect on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.
  
  
  CONCLUSIONS
  Aqueous extract(p18-E3) from the fruit of Eucalyptus maidenii has conspicuous anti-HSV-1 activity. It could inactivate virus directly,and inhibit virus attachment,but had no influence on virus penetration. The mechanism that p18-E3 inactivates virus might involve in viral envelope alteration.
authors.affiliation: Shenzhen People's Hospital, Shenzhen 518020, China.
authors.forename: Huai-qiang
authors.initials: HQ
authors.lastname: Ju
authors.forename: Sha-yan
authors.initials: SY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Pei
authors.forename: Yang-fei
authors.initials: YF
authors.lastname: Xiang
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Ying-jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Chong-ren
authors.initials: CR
authors.lastname: Yang
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Wang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005052:Eucalyptus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000187:drug effects; D006513:Hepatitis B e Antigens / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects*; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012997:Solvents / Q000737:chemistry; D014709:Vero Cells; D010948:Viral Plaque Assay
chemical_list: D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010936:Plant Extracts; D012997:Solvents