title: An ultra-sensitive electrochemical sensor for ascorbic acid based on click chemistry.
abstract: A highly selective and sensitive electrochemical sensor for ascorbic acid (AA) assay has been prepared through Cu(I) catalyzed azide-alkyne cycloaddition reaction (CuAAC). The catalyst, Cu(I) species, is acquired from the reduction of Cu(II) by AA in situ. In the presence of Cu(I) catalyst, the azide modified Au electrode surface is shown to react quantitatively with terminal propargyl-functionalized ferrocene forming 1,2,3-triazoles. The electrochemical response of propargyl-functionalized ferrocene modified Au electrode surface can be monitored using differential pulse voltammetry (DPV) technique. Under optimal conditions, it is found that the current intensity has a linear relationship with the logarithm of AA concentration in the range of 5.0 × 10(-12) to 1.0 × 10(-9) M. Furthermore, the proposed electrochemical sensor shows a good stability (RSD 4.2%), high selectivity and low detection limit for AA detection. In addition, it also demonstrates that the proposed sensor can be applied to detect AA in real urine samples with satisfactory results.
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Suyan
authors.initials: S
authors.lastname: Qiu
authors.forename: Sen
authors.initials: S
authors.lastname: Gao
authors.forename: Lidan
authors.initials: L
authors.lastname: Xie
authors.forename: Hongqin
authors.initials: H
authors.lastname: Chen
authors.forename: Qida
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D057930:Click Chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D013499:Surface Properties
chemical_list: D006046:Gold; D001205:Ascorbic Acid