title: A role for p53 in the response of bystander cells to receipt of medium borne signals from irradiated cells.
abstract: OBJECTIVE
  A number of contradictory studies have reported a role or not for p53 (protein 53) in the production of radiation-induced bystander effects. Most of these studies looked at a range of cell lines with normal or compromised p53 function.
  
  
  METHODS
  In this study, Human Colon Tumour line 116 (HCT 116) cells with confirmed wild type p53 function and a corresponding p53 null HCT 116 line were used to test for bystander signal production and response to bystander signals in a mix/match protocol using the medium transfer technique.
  
  
  RESULTS
  The results showed that both the null cells and the wild type cells produced bystander signals. However, only the p53 wild type cells responded to signals from either cell line. The Human Papilloma Virus transfected keratinocyte line G (HPV-G) reporter cell line used routinely in our laboratory was used to confirm that the null cells were producing signals.
  
  
  CONCLUSIONS
  We conclude that in this system the p53 pathway is involved in response of cells to bystander signals but that signals can be produced by cells which do not have functional p53. If these results apply in vivo, they could be important in radiotherapy where tumours may have compromised p53 function but surrounding (and distant) normal tissue may have wild type functional p53.
authors.affiliation: Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON, Canada. mothers@mcmaster.ca
authors.forename: Carmel
authors.initials: C
authors.lastname: Mothersill
authors.forename: Robert G
authors.initials: RG
authors.lastname: Bristow
authors.forename: Shane M
authors.initials: SM
authors.lastname: Harding
authors.forename: Richard W
authors.initials: RW
authors.lastname: Smith
authors.forename: Anna
authors.initials: A
authors.lastname: Mersov
authors.forename: Colin B
authors.initials: CB
authors.lastname: Seymour
mesh_terms: D024201:Bystander Effect; D002470:Cell Survival / Q000528:radiation effects*; D045325:HCT116 Cells; D006801:Humans; D009369:Neoplasms / Q000532:radiotherapy; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53