title: Distribution of adoptively transferred porcine T-lymphoblasts tracked by (18)F-2-fluoro-2-deoxy-D-glucose and position emission tomography.
abstract: BACKGROUND
  Autologous or allogeneic transfer of tumor-infiltrating T-lymphocytes is a promising treatment for metastatic cancers, but a major concern is the difficulty in evaluating cell trafficking and distribution in adoptive cell therapy. This study presents a method of tracking transfusion of T-lymphoblasts in a porcine model by (18)F-2-fluoro-2-deoxy-d-glucose ([(18)F]FDG) and positron emission tomography.
  
  
  METHODS
  T-lymphoblasts were labeled with the positron-emitting tracer [(18)F]FDG through incubation. The T-lymphoblasts were administered into the bloodstream, and the distribution was followed by positron emission tomography for 120 min. The cells were administered either intravenously into the internal jugular vein (n=5) or intraarterially into the ascending aorta (n=1). Two of the pigs given intravenous administration were pretreated with low-molecular-weight dextran sulphate.
  
  
  RESULTS
  The cellular kinetics and distribution were readily quantifiable for up to 120 min. High (78.6% of the administered cells) heterogeneous pulmonary uptake was found after completed intravenous transfusion. The pulmonary uptake was decreased either by preincubating and coadministrating the T-lymphoblasts with low-molecular-weight dextran sulphate or by administrating them intraarterially.
  
  
  CONCLUSIONS
  The present work shows the feasibility of quantitatively monitoring and evaluating cell trafficking and distribution following administration of [(18)F]FDG-labeled T-lymphoblasts. The protocol can potentially be transferred to the clinical setting with few modifications.
authors.affiliation: Division of Radiology, Department of Oncology, Radiology, Oncology and Radiation Science, Uppsala University, Uppsala 751 87, Sweden. olof.eriksson@radiol.uu.se
authors.forename: Olof
authors.initials: O
authors.lastname: Eriksson
authors.forename: Arian
authors.initials: A
authors.lastname: Sadeghi
authors.forename: Björn
authors.initials: B
authors.lastname: Carlsson
authors.forename: Torsten
authors.initials: T
authors.lastname: Eich
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Lundgren
authors.forename: Bo
authors.initials: B
authors.lastname: Nilsson
authors.forename: Thomas
authors.initials: T
authors.lastname: Tötterman
authors.forename: Olle
authors.initials: O
authors.lastname: Korsgren
authors.forename: Anders
authors.initials: A
authors.lastname: Sundin
mesh_terms: D000818:Animals; D002469:Cell Separation; D016264:Dextran Sulfate / Q000008:administration & dosage / Q000737:chemistry; D019788:Fluorodeoxyglucose F18; D016219:Immunotherapy, Adoptive; D017710:Lymphocyte Transfusion; D008970:Molecular Weight; D049268:Positron-Emission Tomography; D013552:Swine; D013601:T-Lymphocytes / Q000166:cytology / Q000000981:diagnostic imaging* / Q000276:immunology*
chemical_list: D019788:Fluorodeoxyglucose F18; D016264:Dextran Sulfate