title: Development of autoimmune diabetes in the absence of detectable IL-17A in a CD8-driven virally induced model.
abstract: Recent studies have shown that IL-17 can contribute beneficially to pathogen defense but also that excessive IL-17 levels are associated with chronic inflammation and autoimmune disorders. To date, the role of IL-17 in viral infections and type 1 diabetes is ambiguous. In this study, we used IL-17A enhanced green fluorescent protein bicistronic reporter mouse strains to analyze in situ production of IL-17A. Upon Klebsiella pneumoniae bacterial infection, CD4(+) and γδ T cells produce IL-17A. In contrast, CD4(+) or CD8(+) T cells do not produce IL-17A in response to acute or protracted viral infection with lymphocytic choriomeningitis virus or during autoimmune diabetes development in the CD8-driven lymphocytic choriomeningitis virus-induced model of type 1 diabetes. We conclude that viral elimination and type 1 diabetes can occur in the absence of detectable IL-17A production, suggesting IL-17A is not essential in these settings.
authors.affiliation: Diabetes Center of San Diego, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA.
authors.forename: Tom L
authors.initials: TL
authors.lastname: Van Belle
authors.forename: Enric
authors.initials: E
authors.lastname: Esplugues
authors.forename: Jeanette
authors.initials: J
authors.lastname: Liao
authors.forename: Therese
authors.initials: T
authors.lastname: Juntti
authors.forename: Richard A
authors.initials: RA
authors.lastname: Flavell
authors.forename: Matthias G
authors.initials: MG
authors.lastname: von Herrath
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D020381:Interleukin-17 / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014777:Virus Diseases / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D049452:Green Fluorescent Proteins