title: Multiple new site-specific recombinases for use in manipulating animal genomes.
abstract: Site-specific recombinases have been used for two decades to manipulate the structure of animal genomes in highly predictable ways and have become major research tools. However, the small number of recombinases demonstrated to have distinct specificities, low toxicity, and sufficient activity to drive reactions to completion in animals has been a limitation. In this report we show that four recombinases derived from yeast--KD, B2, B3, and R--are highly active and nontoxic in Drosophila and that KD, B2, B3, and the widely used FLP recombinase have distinct target specificities. We also show that the KD and B3 recombinases are active in mice.
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147-2408, USA.
authors.forename: Aljoscha
authors.initials: A
authors.lastname: Nern
authors.forename: Barret D
authors.initials: BD
authors.lastname: Pfeiffer
authors.forename: Karel
authors.initials: K
authors.lastname: Svoboda
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Rubin
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics; D015870:Gene Expression; D016678:Genome / Q000235:genetics*; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases; C077570:Site-specific recombinase