title: Conserved peptide sequences bind to actin and enolase on the surface of Plasmodium berghei ookinetes.
abstract: The description of Plasmodium ookinete surface proteins and their participation in the complex process of mosquito midgut invasion is still incomplete. In this study, using phage display, a consensus peptide sequence (PWWP) was identified in phages that bound to the Plasmodium berghei ookinete surface and, in selected phages, bound to actin and enolase in overlay assays with ookinete protein extracts. Actin was localized on the surface of fresh live ookinetes by immunofluorescence and electron microscopy using specific antibodies. The overall results indicated that enolase and actin can be located on the surface of ookinetes, and suggest that they could participate in Plasmodium invasion of the mosquito midgut.
authors.affiliation: Centro de Investigación sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Avenida Universidad No. 655, Col. Santa María Ahuacatitlán, Cuernavaca, Morelos, CP62508 México.
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Romano
authors.forename: M H
authors.initials: MH
authors.lastname: Rodríguez
authors.forename: V
authors.initials: V
authors.lastname: Pando
authors.forename: J A
authors.initials: JA
authors.lastname: Torres-Monzón
authors.forename: A
authors.initials: A
authors.lastname: Alvarado-Delgado
authors.forename: A N
authors.initials: AN
authors.lastname: Lecona Valera
authors.forename: R Argotte
authors.initials: RA
authors.lastname: Ramos
authors.forename: J
authors.initials: J
authors.lastname: Martínez-Barnetche
authors.forename: M C
authors.initials: MC
authors.lastname: Rodríguez
mesh_terms: D000199:Actins / Q000378:metabolism*; D000330:Aedes / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology; D002457:Cell Extracts; D002478:Cells, Cultured; D017124:Conserved Sequence; D004064:Digestive System / Q000378:metabolism* / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D008018:Life Cycle Stages / Q000235:genetics*; D008288:Malaria / Q000378:metabolism / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010962:Plasmodium berghei / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000378:metabolism; D012376:Rodent Diseases / Q000378:metabolism / Q000469:parasitology
chemical_list: D000199:Actins; D002457:Cell Extracts; D019151:Peptide Library; D010455:Peptides; D015800:Protozoan Proteins; D010751:Phosphopyruvate Hydratase