title: [The relationship between endoplasmic reticulum stress and its particular apoptosis way caspase-12 and apoptosis in renal cortex of diabetic rats].
abstract: OBJECTIVE
  To investigate the expressions of 78-kDa glucose-regulated protein (GRP78) and Caspase-12 and their relationship with apoptosis in renal cortex of diabetic rats.
  
  
  METHODS
  Uninephrectomized Wistar rats were used to induce diabetes by intraperitoneal injection of Streptozotocin (STZ 65 mg/kg). After 8 weeks, the expression and distribution of GRP78, Caspase-12, proliferating cell nuclear antigen (PCNA) were examined by immunohistochemistry. Flow cytometry was used to detect the levels of protein of GRP78 and Caspase-12. Apoptosis was evaluated by means of terminal deoxynucleotidyl transferase-mediated d-UDP nick-end labeling (TUNEL) and Flow cytometry. Serum creatinine, blood urea nitrogen and 24-hour urine protein excretion were checked.
  
  
  RESULTS
  Compared with those in normal control group, the numbers of apoptosis and the expression of GRP78, Caspase-12 in glomerular and tubular cells were much higher in the diabetic kidneys at 8 weeks. There was no significant difference between group A and group B.
  
  
  CONCLUSIONS
  Activation of endoplasmic reticulum stress may play an important role in the development of diabetic nephropathy.
authors.affiliation: Department of pathology, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Cao
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Hao
authors.forename: Qing-Juan
authors.initials: QJ
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Duan
mesh_terms: D000818:Animals; D017209:Apoptosis; D053454:Caspase 12 / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D003928:Diabetic Nephropathies / Q000473:pathology*; D059865:Endoplasmic Reticulum Stress; D006360:Heat-Shock Proteins / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism / Q000473:pathology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D053454:Caspase 12; D006360:Heat-Shock Proteins; D018809:Proliferating Cell Nuclear Antigen; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP; C506443:Casp12 protein, rat