title: Comparison of innate immune responses to pathogenic and putative non-pathogenic hantaviruses in vitro.
abstract: Hantaviruses are human pathogens that cause hemorrhagic fever with renal syndrome or hantavirus cardiopulmonary syndrome. The mechanisms accounting for the differences in virulence between pathogenic and non-pathogenic hantaviruses are not well known. We have examined the pathogenesis of different hantavirus groups by comparing the innate immune responses induced in the host cell following infection by pathogenic (Sin Nombre, Hantaan, and Seoul virus) and putative non-pathogenic (Prospect Hill, Tula, and Thottapalayam virus) hantaviruses. Pathogenic hantaviruses were found to replicate more efficiently in interferon-competent A549 cells than putative non-pathogenic hantaviruses. The former also suppressed the expression of the interferon-β and myxovirus resistance protein genes, while the transcription level of both genes increased rapidly within 24 h post-infection in the latter. In addition, the induction level of interferon correlated with the activation level of interferon regulatory factor-3. Taken together, these results suggest that the observed differences are correlated with viral pathogenesis and further indicate that pathogenic and putative non-pathogenic hantaviruses differ in terms of early interferon induction via activation of the interferon regulatory factor-3 in infected host cells.
authors.affiliation: Department of Microbiology, Institute for Viral Diseases, College of Medicine, Korea University, Seoul 136-705, Republic of Korea.
authors.forename: So Hee
authors.initials: SH
authors.lastname: Shim
authors.forename: Man-Seong
authors.initials: MS
authors.lastname: Park
authors.forename: Sungsil
authors.initials: S
authors.lastname: Moon
authors.forename: Kwang Sook
authors.initials: KS
authors.lastname: Park
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Song
authors.forename: Ki-Joon
authors.initials: KJ
authors.lastname: Song
authors.forename: Luck Ju
authors.initials: LJ
authors.lastname: Baek
mesh_terms: D002460:Cell Line; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D005786:Gene Expression Regulation; D006476:Orthohantavirus / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D007113:Immunity, Innate; D016899:Interferon-beta / Q000037:antagonists & inhibitors / Q000235:genetics; D064547:Myxovirus Resistance Proteins; D014158:Transcription, Genetic; D014779:Virus Replication
chemical_list: D064547:Myxovirus Resistance Proteins; D016899:Interferon-beta; D019204:GTP-Binding Proteins