title: Immune amplification of murine CD8 suppressor T cells induced via an immune-privileged site: quantifying suppressor T cells functionally.
abstract: BACKGROUND
  CD8(+) suppressor T cells exert antigen-specific suppression of the expression of hypersensitivity by activated T cells. Therefore, CD8(+) suppressor T cells serve a major regulatory role for the control of active immunity. Accordingly, the number and/or activity of CD8(+) suppressor T cells should be influenced by an immune response to the antigen. To test this hypothesis we used an adoptive transfer assay that measures the suppression of the expression of delayed-type hypersensitivity (DTH) by CD8(+) suppressor T cells to quantify the antigen-specific suppression of DTH by these suppressor T cells.
  
  
  METHODS
  Suppressor T cells were induced in the spleens of mice by the injection of antigen into the anterior chamber of an eye. Following this injection, the mice were immunized by the same antigen injected into the anterior chamber. Spleen cells recovered from these mice (AC-SPL cells) were titrated in an adoptive transfer assay to determine the number of AC-SPL cells required to effect a 50% reduction of antigen-induced swelling (Sw50) in the footpad of immunized mice challenged by antigen.
  
  
  RESULTS
  Suppression of the expression of DTH is proportional to the number of AC-SPL cells injected into the site challenged by antigen. The number of AC-SPL cells required for a 50% reduction in DTH-induced swelling is reduced by injecting a cell population enriched for CD8(+) AC-SPL cells. Immunizing the mice receiving intracameral antigen to the same antigen decreases the RSw50 of AC-SPL cells required to inhibit the expression of DTH.
  
  
  CONCLUSIONS
  The results provide the first quantitative demonstration that the numbers of antigen-specific splenic CD8(+) suppressor T cells are specifically amplified by antigen during an immune response.
authors.affiliation: Department of Immunology, University of Connecticut Health Center, Farmington, Connecticut, United States of America.
authors.forename: Roshanak
authors.initials: R
authors.lastname: Sharafieh
authors.forename: Yen
authors.initials: Y
authors.lastname: Lemire
authors.forename: Sabrina
authors.initials: S
authors.lastname: Powell
authors.forename: James
authors.initials: J
authors.lastname: O'Rourke
authors.forename: Robert E
authors.initials: RE
authors.lastname: Cone
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000867:Anterior Chamber; D000941:Antigens / Q000008:administration & dosage; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000941:Antigens