title: Tissue/fluid correlation study for the depletion of sulfadimethoxine in bovine kidney, liver, plasma, urine, and oral fluid.
abstract: Sulfonamides are among the oldest, but still effective, antimicrobial veterinary medicines. In steers and dairy cows, the sulfonamides are effective in the treatment of respiratory disease and general infections. Sulfadimethoxine (SDM) has been approved by US Food and Drug Administration (FDA) for use in steers and dairy cows with a tolerance of 100 ng/g (ppb) in edible tissues and 10 ppb in milk. The detection of SDM residue above tolerance in the animal slaughtered for food process will result in the whole carcass being discarded. This report describes a comprehensive depletion study of SDM (and its main metabolite) in plasma, urine, oral fluid, kidney, and liver. In this study, nine steers were injected intravenously with the approved dose of SDM; the loading dose was 55 mg/kg, followed by 27.5 mg/kg dose at 24 h and again at 48 h. Fluids (blood, urine, and saliva) and tissue (liver and kidney) samples were collected at intervals after the last dose of SMD. The combination of laparoscopic serial sampling technique with the liquid chromatography/mass spectrometry method provided the data to establish the tissue/fluid correlation in the depletion of SMD. A strong correlation and linearity of the log-scale concentration over time in the depletion stage has been confirmed for kidney, liver, and plasma.
authors.affiliation: Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Division of Applied Veterinary Research, Laurel, MD 20708, USA. oscar.chiesa@fda.hhs.gov
authors.forename: O A
authors.initials: OA
authors.lastname: Chiesa
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: P J
authors.initials: PJ
authors.lastname: Kijak
authors.forename: J X
authors.initials: JX
authors.lastname: Li
authors.forename: V
authors.initials: V
authors.lastname: Lancaster
authors.forename: M L
authors.initials: ML
authors.lastname: Smith
authors.forename: D N
authors.initials: DN
authors.lastname: Heller
authors.forename: M H
authors.initials: MH
authors.lastname: Thomas
authors.forename: J
authors.initials: J
authors.lastname: Von Bredow
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D001706:Biopsy / Q000662:veterinary; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D005260:Female; D007275:Injections, Intravenous / Q000662:veterinary; D007668:Kidney / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013412:Sulfadimethoxine / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine
chemical_list: D000890:Anti-Infective Agents; D013412:Sulfadimethoxine