title: Infection of cynomolgus macaques with a recombinant monkeypox virus encoding green fluorescent protein.
abstract: Monkeypox virus (MPXV) causes a vesiculopustular rash illness resembling smallpox in humans and produces a similar disease in nonhuman primates. To enhance the ability of researchers to study experimental MPXV infections, we inserted a gene encoding green fluorescent protein (GFP) into Monkeypox virus Zaire-79. Wild-type and MPXV-GFP replicated with similar kinetics in cell culture and caused a similar disease when injected intravenously into cynomolgus macaques. In MPXV-GFP-infected animals, examination under fluorescent light facilitated the identification of skin lesions during disease development and internal sites of replication at necropsy. MPXV-GFP could improve the quantitative assessment of antiviral therapy and vaccine efficacy.
authors.affiliation: Virology Division, Viral Therapeutics Branch, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702-5011, USA. arthur.goff@amedd.army.mil
authors.forename: Arthur
authors.initials: A
authors.lastname: Goff
authors.forename: Eric
authors.initials: E
authors.lastname: Mucker
authors.forename: Jolynne
authors.initials: J
authors.lastname: Raymond
authors.forename: Robert
authors.initials: R
authors.lastname: Fisher
authors.forename: Mike
authors.initials: M
authors.lastname: Bray
authors.forename: Lisa
authors.initials: L
authors.lastname: Hensley
authors.forename: Jason
authors.initials: J
authors.lastname: Paragas
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008252:Macaca fascicularis; D045908:Mpox, Monkeypox / Q000821:virology; D008993:Monkeypox virus / Q000235:genetics / Q000502:physiology*; D012899:Smallpox / Q000821:virology*
chemical_list: D049452:Green Fluorescent Proteins