title: Progress in prokaryotic transcriptomics.
abstract: Genome-wide expression studies transformed the field of transcriptomics and made it feasible to study global gene expression in extraordinary detail. These new methods have revealed an enhanced view of the transcriptional landscape and have yielded many biological insights. It is increasingly clear that the prokaryotic transcriptome is much more complex than once thought. Recent advances in microbial transcriptome analyses are highlighted in this review. Areas of progress include the development of optimized techniques that minimize the abundance of ribosomal RNAs in RNA samples as well as the development of novel methods to create transcriptome libraries. Advances such as these have led to a new emphasis in areas such as metatranscriptomics and single cell gene expression studies.
authors.affiliation: Plant-Microbe Interactions Research Unit, USDA-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY 14853, United States. Melanie.Filiatrault@ars.usda.gov
authors.forename: Melanie J
authors.initials: MJ
authors.lastname: Filiatrault
mesh_terms: D001419:Bacteria / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008967:Molecular Biology / Q000379:methods; D011387:Prokaryotic Cells; D059467:Transcriptome