title: [The CD133 polyclonal antibody generation and cancer stem cells identification].
abstract: OBJECTIVE
  To generate the cancer stem cells (CSCs) specific protein CD133 polyclonal antibody for the study of the biological characteristics of CSCs in tumor tissues and CSCs screening for the mouse model.
  
  
  METHODS
  The extracellular peptide of the human CD133 was injected into rabbits to generate polyclonal antibody which was used for glioblastoma(GBM) Western blot and immunohistochemistry.
  
  
  RESULTS
  The CD133 antiserum we made could detect both overexpressed myc-CD133 and endogenous CD133 efficiently by Western blot. Immunohistochemistry indicated that the CD133 polyclonal antibody can label CSCs in GBM sections.
  
  
  CONCLUSIONS
  High efficient and specific CD133 antibody was generated successfully and could be used to label CSCs in tumor sections and screen CSCs for the mouse model.
authors.affiliation: State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jun-Long
authors.initials: JL
authors.lastname: Tian
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Cai
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Xia
authors.forename: Fang-Hua
authors.initials: FH
authors.lastname: Li
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Wang
authors.forename: Mi-Na
authors.initials: MN
authors.lastname: Chen
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D045744:Cell Line, Tumor; D006023:Glycoproteins / Q000276:immunology*; D056945:Hep G2 Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D014411:Neoplastic Stem Cells / Q000276:immunology* / Q000473:pathology*; D010455:Peptides / Q000276:immunology*; D011817:Rabbits
chemical_list: D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse