title: Activity of Cuban propolis extracts on Leishmania amazonensis and Trichomonas vaginalis.
abstract: In this paper we analyzed the antiprotozoal effects of eighteen Cuban propolis extracts (brown, red and yellow type) collected in different geographic areas, using Leishmania amazonensis (as a model of intracellular protozoa) and Trichomonas vaginalis (as a model of extracellular protozoa). All evaluated propolis extracts caused inhibitory effect on intracellular amastigotes of L. amazonensis. However, cytotoxicity on peritoneal macrophages from BALB/c mice was observed. Only five samples decreased the viability of T. vaginalis trophozoites at concentrations lower than 10 microg/mL. No correlation between the type of propolis and antiprotozoal activity was found. Cuban propolis extracts demonstrated activity against both intracellular and extracellular protozoa model, as well as the potentialities of propolis as a natural source to obtain new antiprotozoal agents.
authors.affiliation: Parasitology Department, Institute of Tropical Medicine "Pedro Kouri", Havana City, Cuba. monzote@ipk.sld.cu
authors.forename: Lianet
authors.initials: L
authors.lastname: Monzote Fidalgo
authors.forename: Idalia
authors.initials: I
authors.lastname: Sariego Ramos
authors.forename: Marley
authors.initials: M
authors.lastname: García Parra
authors.forename: Osmany
authors.initials: O
authors.lastname: Cuesta-Rubio
authors.forename: Ingrid
authors.initials: I
authors.lastname: Márquez Hernández
authors.forename: Mercedes
authors.initials: M
authors.lastname: Campo Fernández
authors.forename: Anna Lisa
authors.initials: AL
authors.lastname: Piccinelli
authors.forename: Luca
authors.initials: L
authors.lastname: Rastrelli
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003462:Cuba; D007891:Leishmania / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D021261:Parasitic Sensitivity Tests; D011429:Propolis / Q000494:pharmacology* / Q000633:toxicity; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D011429:Propolis