title: Human mesenchymal stem cells seeded on extracellular matrix-scaffold: viability and osteogenic potential.
abstract: The development and the optimization of novel culture systems of mesenchymal osteoprogenitors are some of the most important challenges in the field of bone tissue engineering (TE). A new combination between cells and extracellular matrix (ECM)-scaffold, containing ECM has here been analyzed. As source for osteoprogenitors, mesenchymal stem cells obtained from human umbilical cord Wharton's Jelly (hWJMSCs), were used. As ECM-scaffold, a powder form of isolated and purified porcine urinary bladder matrix (pUBM), was employed. The goals of the current work were: (1) the characterization of the in vitro hWJMSCs behavior, in terms of viability, proliferation, and adhesion to ECM-scaffold; (2) the effectiveness of ECM-scaffold to induce/modulate the osteoblastic differentiation; and (3) the proposal for a possible application of cells/ECM-scaffold construct to the field of cell/TE. In this respect, the properties of the pUBM-scaffold in promoting and guiding the in vitro adhesion, proliferation, and three-dimensional colonization of hWJMSCs, without altering viability and morphological characteristics of the cells, are here described. Finally, we have also demonstrated that pUBM-scaffolds positively affect the expression of typical osteoblastic markers in hWJMSCs.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy.
authors.forename: Letizia
authors.initials: L
authors.lastname: Penolazzi
authors.forename: Stefania
authors.initials: S
authors.lastname: Mazzitelli
authors.forename: Renata
authors.initials: R
authors.lastname: Vecchiatini
authors.forename: Elena
authors.initials: E
authors.lastname: Torreggiani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Lambertini
authors.forename: Scott
authors.initials: S
authors.lastname: Johnson
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
authors.forename: Roberta
authors.initials: R
authors.lastname: Piva
authors.forename: Claudio
authors.initials: C
authors.lastname: Nastruzzi
mesh_terms: D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D003470:Culture Media; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D054457:Tissue Scaffolds; D059631:Wharton Jelly / Q000166:cytology*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D051176:beta Catenin; D019938:Cyclin D1; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13