title: Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate.
abstract: BACKGROUND
  There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate.
  
  
  METHODS
  Primary tubes containing whole blood were frozen at -20°C and -80°C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma.
  
  
  CONCLUSIONS
  Massive amount of blood cells lysis could only be obtained by storing samples 12h at -20°C, or 2h at -80°C.
authors.affiliation: U.O. Diagnostica Ematochimica, Dipartimento di Patologia e Medicina di Laboratorio, Azienda Ospedaliero-Universitaria di Parma, Italy. glippi@ao.pr.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lippi
authors.forename: Roberta
authors.initials: R
authors.lastname: Musa
authors.forename: Rosalia
authors.initials: R
authors.lastname: Aloe
authors.forename: Mariella
authors.initials: M
authors.lastname: Mercadanti
authors.forename: Silvia
authors.initials: S
authors.lastname: Pipitone
mesh_terms: D001773:Blood Cells; D001793:Blood Preservation / Q000379:methods*; D005615:Freezing; D006801:Humans; D013696:Temperature; D013997:Time Factors