title: [Study on methods of deproteinization from Phellinus baumii polysaccharide].
abstract: OBJECTIVE
  To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide.
  
  
  METHODS
  Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii, respectively.
  
  
  RESULTS
  The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows: 30 min treating time, 5% trichloroacetic acid,3 times treatemt. Under these conditions the rate of protein-removed was 82% and the rate of polysaccharide-lost was 10.8%.
  
  
  CONCLUSIONS
  TCA method is the optimal mean for deproteinization from Phellinus baumii polysaccharide.
authors.affiliation: Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. xieliyuan77@163.com
authors.forename: Li-yuan
authors.initials: LY
authors.lastname: Xie
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Peng
authors.forename: Bing-cheng
authors.initials: BC
authors.lastname: Gan
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D002725:Chloroform / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*; D013678:Technology, Pharmaceutical / Q000379:methods; D014238:Trichloroacetic Acid / Q000737:chemistry
chemical_list: D011134:Polysaccharides; D011506:Proteins; D014238:Trichloroacetic Acid; D002725:Chloroform