title: Study of immunological aspects of aspergillosis in mice and effect of polyene macrolide antibiotic (SJA-95) and IFN-γ: a possible role of IFN-γ as an adjunct in antifungal therapy.
abstract: New polyene macrolide antibiotic SJA-95 in free as well as liposomal (lip.) forms, with and without interferon-γ (IFN-γ) was studied in mice model of aspergillosis using biological and biochemical parameters viz. colony forming units (CFU) in liver, spleen, kidney, lung and brain, and serum IgG, and interleukin-4 (IL-4). Treatment with free and lip SJA-95 along with IFN-γ prolonged the survival time, reduced CFU in vital organs, decreased serum IgG and IL-4 levels. SJA-95 lip form showed greater antifungal activity as compared to free form. The combined treatment of lip SJA-95 with IFN-γ showed further enhancement in antifungal activity of SJA-95 (lip). The present experimental findings demonstrated IFN-γ might act as a potent modulator in immune reaction during fungal infection and can be a useful adjunctive in antifungal therapy in the management of deep seated systemic mycoses.
authors.affiliation: Department of Pharmacology, Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk), Lonavala, Pune, India. srnaik5@rediffmail.com
authors.forename: Suresh R
authors.initials: SR
authors.lastname: Naik
authors.forename: Vishnu N
authors.initials: VN
authors.lastname: Thakare
authors.forename: Sandhya K
authors.initials: SK
authors.lastname: Desai
authors.forename: Prabhakar R
authors.initials: PR
authors.lastname: Rahalkar
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D001228:Aspergillosis / Q000188:drug therapy / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D056747:Immunomodulation / Q000187:drug effects*; D007371:Interferon-gamma / Q000008:administration & dosage*; D015847:Interleukin-4 / Q000097:blood; D008081:Liposomes / Q000008:administration & dosage; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D018942:Macrolides / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D011090:Polyenes / Q000008:administration & dosage*; D013154:Spleen / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D007074:Immunoglobulin G; D008081:Liposomes; D018942:Macrolides; D011090:Polyenes; C519970:SJA-95; D015847:Interleukin-4; D007371:Interferon-gamma