title: Phytochemical, antioxidant, and antiproliferative properties of seed oil and flour extracts of Maryland-grown tobacco cultivars.
abstract: To determine the possible alternative use of tobacco, the seeds representing seven Maryland tobacco cultivars were investigated for their phytochemical, antioxidant, and antiproliferative properties. Tobacco seed oils were extracted by the Soxhlet method, and analyzed for their yield, density, refractive index, fatty acid profiles, and tocopherol profile. The defatted flours were extracted in 50% acetone and 80% ethanol. The tobacco seed oil and flour extracts were analyzed for total phenolic contents (TPC) and scavenging capacities against peroxyl, hydroxyl and 2,2-diphenyl-1-picryhydrazyl (DPPH) radicals. The fatty acid compositions of phospholipids and the protein content of the flours were also analyzed. In addition, oil and flour extracts of varieties MD609 and MD609LA were evaluated for their antiproliferative effects on HT-29 human colon cancer cells. All of the tested extracts significantly inhibited HT-29 cell proliferation except that from MD609 oil. The data from this study suggest the potential alternative use of tobacco seeds in developing natural antioxidants and antiproliferative agents for improving human health.
authors.affiliation: Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742, United States.
authors.forename: Zhuohong
authors.initials: Z
authors.lastname: Xie
authors.forename: Monica
authors.initials: M
authors.lastname: Whent
authors.forename: Herman
authors.initials: H
authors.lastname: Lutterodt
authors.forename: Yuge
authors.initials: Y
authors.lastname: Niu
authors.forename: Margaret
authors.initials: M
authors.lastname: Slavin
authors.forename: Robert
authors.initials: R
authors.lastname: Kratochvil
authors.forename: Liangli Lucy
authors.initials: LL
authors.lastname: Yu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis*; D049109:Cell Proliferation / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis; D016166:Free Radical Scavengers / Q000032:analysis; D019073:HT29 Cells; D006801:Humans; D008396:Maryland; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014026:Nicotiana / Q000737:chemistry*; D024505:Tocopherols / Q000032:analysis
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D005227:Fatty Acids; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D010938:Plant Oils; D024505:Tocopherols