title: FUS and TARDBP but not SOD1 interact in genetic models of amyotrophic lateral sclerosis.
abstract: Mutations in the SOD1 and TARDBP genes have been commonly identified in Amyotrophic Lateral Sclerosis (ALS). Recently, mutations in the Fused in sarcoma gene (FUS) were identified in familial (FALS) ALS cases and sporadic (SALS) patients. Similarly to TDP-43 (coded by TARDBP gene), FUS is an RNA binding protein. Using the zebrafish (Danio rerio), we examined the consequences of expressing human wild-type (WT) FUS and three ALS-related mutations, as well as their interactions with TARDBP and SOD1. Knockdown of zebrafish Fus yielded a motor phenotype that could be rescued upon co-expression of wild-type human FUS. In contrast, the two most frequent ALS-related FUS mutations, R521H and R521C, unlike S57Δ, failed to rescue the knockdown phenotype, indicating loss of function. The R521H mutation caused a toxic gain of function when expressed alone, similar to the phenotype observed upon knockdown of zebrafish Fus. This phenotype was not aggravated by co-expression of both mutant human TARDBP (G348C) and FUS (R521H) or by knockdown of both zebrafish Tardbp and Fus, consistent with a common pathogenic mechanism. We also observed that WT FUS rescued the Tardbp knockdown phenotype, but not vice versa, suggesting that TARDBP acts upstream of FUS in this pathway. In addition we observed that WT SOD1 failed to rescue the phenotype observed upon overexpression of mutant TARDBP or FUS or upon knockdown of Tardbp or Fus; similarly, WT TARDBP or FUS also failed to rescue the phenotype induced by mutant SOD1 (G93A). Finally, overexpression of mutant SOD1 exacerbated the motor phenotype caused by overexpression of mutant FUS. Together our results indicate that TARDBP and FUS act in a pathogenic pathway that is independent of SOD1.
authors.affiliation: Department of Pathology and Cell Biology and Groupe de Recherche Sur le Système Nerveux Central, University of Montreal, Montreal, Canada. edor.kabashi@icm-institute.org
authors.forename: Edor
authors.initials: E
authors.lastname: Kabashi
authors.forename: Valérie
authors.initials: V
authors.lastname: Bercier
authors.forename: Alexandra
authors.initials: A
authors.lastname: Lissouba
authors.forename: Meijiang
authors.initials: M
authors.lastname: Liao
authors.forename: Edna
authors.initials: E
authors.lastname: Brustein
authors.forename: Guy A
authors.initials: GA
authors.lastname: Rouleau
authors.forename: Pierre
authors.initials: P
authors.lastname: Drapeau
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004843:Epistasis, Genetic; D006801:Humans; D008957:Models, Genetic; D009043:Motor Activity / Q000235:genetics; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D034702:RNA-Binding Protein FUS / Q000235:genetics* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D000072105:Superoxide Dismutase-1; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D034702:RNA-Binding Protein FUS; C000606290:SOD1 protein, human; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1