title: The effects of Lonomin V, a toxin from the caterpillar (Lonomia achelous), on hemostasis parameters as measured by platelet function.
abstract: Platelets play a central role in hemostasis during vascular injury. Patients affected with the hemorrhagic syndrome caused by contact with Lonomia achelous caterpillars (Lac) Lepidoptera distributed in various South American countries, show digestive, pulmonary and intraperitoneal bleeding in combination with hematomas and echymosis. In the present study, we have evaluated the effects of Lonomin V (serine protease isolated from Lac hemolymph) on some functional properties of platelets, evaluating its importance in primary hemostasis. Platelet adhesion to fibrinogen was reduced by 19, 20, 36, and 37% after pre-treated with 0.2, 2, 20 and 40 nM of Lonomin V, respectively. Pre-incubation of the platelets with 408 nM of Lonomin V, for 4 min at 37 °C, resulted in complete inhibition of the collagen-induced platelet aggregation, in contrast to 56% inhibition of the ADP - induced platelet aggregation. Lonomin V also inhibited anti-α(IIb)β(3) integrin binding to platelets by 56, 57, 52 and 54% at concentrations of 0.2, 2, 20 and 40 nM respectively. Additionally, Lonomin V inhibited anti-P-selectin binding to platelets by 28, 37, 33 and 33% at the same concentrations. The platelets tested with Lonomin V did not modify their viability. In summary, Lonomin V inhibited platelet aggregation, probably caused by the degradation of collagen. The anti-platelet activity of Lonomin V has been shown to be unique and a potentially useful tool for investigating cell-matrix and cell-cell interactions and for the development of antithrombotic agents in terms of their anti-adhesive activities.
authors.affiliation: Laboratorio de Fisiopatología, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas, Apartado 20632, Caracas 1020A, Republic of Venezuela.
authors.forename: Belsy
authors.initials: B
authors.lastname: Guerrero
authors.forename: Carmen L
authors.initials: CL
authors.lastname: Arocha-Piñango
authors.forename: Ana M
authors.initials: AM
authors.lastname: Salazar
authors.forename: Amparo
authors.initials: A
authors.lastname: Gil
authors.forename: Elda E
authors.initials: EE
authors.lastname: Sánchez
authors.forename: Alexis
authors.initials: A
authors.lastname: Rodríguez-Acosta
authors.forename: Sara
authors.initials: S
authors.lastname: Lucena
mesh_terms: D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D006487:Hemostasis / Q000187:drug effects*; D006801:Humans; D007814:Larva / Q000737:chemistry*; D009036:Moths / Q000737:chemistry* / Q000254:growth & development; D019007:P-Selectin / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism
chemical_list: D001180:Arthropod Venoms; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C108044:lonomin V; D005340:Fibrinogen; D003094:Collagen