title: Improvement of Phytase Activity by a New Saccharomyces cerevisiae Strain Using Statistical Optimization.
abstract: Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2(2) full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae.
authors.affiliation: Food Science Department, Faculty of Food Engineering, Campinas State University (UNICAMP), Monteiro Lobato Street 70, 13083 970 Campinas, SP, Brazil.
authors.forename: Edi Franciele
authors.initials: EF
authors.lastname: Ries
authors.forename: Gabriela
authors.initials: G
authors.lastname: Alves Macedo