title: Checkpoints in adenoviral production: cross-contamination and E1A.
abstract: Adenoviruses are widely used for overexpressing proteins in primary mammalian cells. Incorporation of the early viral gene, E1A, or viral cross-contamination can occur during amplification, and identification of these products is crucial as the transcription of unwanted genetic material can impact cell function and compromise data interpretation. Here we report methods for evaluation of contaminating adenovirus and E1 viral DNA.
authors.affiliation: Vascular Biology Section, Boston University Medical Center, Boston, Massachusetts, United States of America.
authors.forename: Dagmar J
authors.initials: DJ
authors.lastname: Haeussler
authors.forename: Alicia M
authors.initials: AM
authors.lastname: Evangelista
authors.forename: Joseph R
authors.initials: JR
authors.lastname: Burgoyne
authors.forename: Richard A
authors.initials: RA
authors.lastname: Cohen
authors.forename: Markus M
authors.initials: MM
authors.lastname: Bachschmid
authors.forename: David R
authors.initials: DR
authors.lastname: Pimental
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology*; D017498:Adenovirus E1 Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans
chemical_list: D017498:Adenovirus E1 Proteins; D004279:DNA, Viral