title: Rapid response of the yeast plasma membrane proteome to salt stress.
abstract: The plasma membrane separates the cell from the external environment and plays an important role in the stress response of the cell. In this study, we compared plasma membrane proteome modifications of yeast cells exposed to mild (0.4 m NaCl) or high (1 m NaCl) salt stress for 10, 30, or 90 min. Plasma membrane-enriched fractions were isolated, purified, and subjected to iTRAQ labeling for quantitative analysis. In total, 88-109 plasma membrane proteins were identified and quantified. The quantitative analysis revealed significant changes in the abundance of several plasma membrane proteins. Mild salt stress caused an increase in abundance of 12 plasma membrane proteins, including known salt-responsive proteins, as well as new targets. Interestingly, 20 plasma membrane proteins, including the P-type H(+)-ATPase Pma1, ABC transporters, glucose and amino acid transporters, t-SNAREs, and proteins involved in cell wall biogenesis showed a significant and rapid decrease in abundance in response to both 0.4 m and 1 m NaCl. We propose that rapid protein internalization occurs as a response to hyper-osmotic and/or ionic shock, which might affect plasma membrane morphology and ionic homeostasis. This rapid response might help the cell to survive until the transcriptional response takes place.
authors.affiliation: Université Catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 5/15, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szopinska
authors.forename: Hervé
authors.initials: H
authors.lastname: Degand
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Hochstenbach
authors.forename: Joseph
authors.initials: J
authors.lastname: Nader
authors.forename: Pierre
authors.initials: P
authors.lastname: Morsomme
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009997:Osmotic Pressure; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012965:Sodium Chloride; D013312:Stress, Physiological
chemical_list: C066709:GAS1 protein, S cerevisiae; C487328:LSP1 protein, S cerevisiae; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C487327:PIL1 protein, S cerevisiae; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D012965:Sodium Chloride