title: Exploring Tagetes erecta Linn flower for the elastase, hyaluronidase and MMP-1 inhibitory activity.
abstract: BACKGROUND
  Traditionally Tagetes erecta Linn flower is claimed to treat skin diseases like sores, burns, wounds, ulcers, eczema and several other skin ailments. The aim of the present experiment was to evaluate the anti-wrinkle potential of standardized flower extract of Tagetes erecta.
  
  
  METHODS
  The Tagetes erecta extract and fractions were screened for hyaluronidase, elastase and matrix metalloproteinase (MMP-1) inhibitory activity compared with the activity of standard oleanolic acid. Syringic acid and β-amyrin were obtained from the extract and quantified through RP-HPLC. Also the compounds were evaluated for anti-wrinkle activity.
  
  
  RESULTS
  The methanol extract showed significant ((a)P < 0.05) hyaluronidase and elastase inhibition with IC50 of 11.70 ± 1.79 μg mL(-1) and 4.13 ± 0.93 μg mL(-1) respectively and better MMP-1 inhibition compared to standard oleanolic acid. The isolated compounds syringic acid and β-amyrin found to inhibit enzymes comparable to oleanolic acid. The RP-HPLC analysis revealed that good amounts of syringic acid and β-amyrin (2.30%, w/w and 0.06%) are present in Tagetes erecta.
  
  
  CONCLUSIONS
  Tagetes erecta flower showed effective inhibition of hyaluronidase, elastase and MMP-1. Therefore, this experiment further rationalizes the traditional uses of this plant, which may be useful as an anti-wrinkle agent.
authors.affiliation: School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
authors.forename: Niladri
authors.initials: N
authors.lastname: Maity
authors.forename: Neelesh K
authors.initials: NK
authors.lastname: Nema
authors.forename: Md Karim
authors.initials: MK
authors.lastname: Abedy
authors.forename: Birendra K
authors.initials: BK
authors.lastname: Sarkar
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D000432:Methanol / Q000737:chemistry; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011480:Protease Inhibitors / Q000494:pharmacology; D015595:Skin Aging / Q000187:drug effects*; D012997:Solvents / Q000737:chemistry; D031686:Tagetes / Q000737:chemistry
chemical_list: D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D011480:Protease Inhibitors; D012997:Solvents; D005707:Gallic Acid; D009828:Oleanolic Acid; C001945:syringic acid; D006821:Hyaluronoglucosaminidase; D019272:Leukocyte Elastase; D020781:Matrix Metalloproteinase 1; C036380:beta-amyrin; D000432:Methanol