title: Immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571 using modified sodium alginate beads.
abstract: An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of glutaraldehyde and 30 g/L of activated coal led to the highest specific activity (2,063.5 U/mg of protein), corresponding to an enhancement of about 26 times compared to the activity of the free enzyme (79.1 U/mg of protein). The effect of pH and temperature on the immobilized enzyme activity was also evaluated, showing optimal activities at pH of 5.5 and 55 °C. The study of storage of immobilized inulinase in different temperatures showed that the extract kept its initial activity after 43 days of storage at 40 and 50 °C and after 138 days of storage either at 4 or 25 °C.
authors.affiliation: Campus de Erechim, Universidade Regional Integrada do Alto Uruguai e das Missões, URI, Av. Sete de Setembro 1621, Erechim, RS, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Richetti
authors.forename: Cristiane B
authors.initials: CB
authors.lastname: Munaretto
authors.forename: Lindomar A
authors.initials: LA
authors.lastname: Lerin
authors.forename: Luciane
authors.initials: L
authors.lastname: Batistella
authors.forename: J
authors.initials: J
authors.lastname: Vladimir Oliveira
authors.forename: Rogério M
authors.initials: RM
authors.lastname: Dallago
authors.forename: Viviane
authors.initials: V
authors.lastname: Astolfi
authors.forename: Marco Di
authors.initials: MD
authors.lastname: Luccio
authors.forename: Márcio A
authors.initials: MA
authors.lastname: Mazutti
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
authors.forename: Helen
authors.initials: H
authors.lastname: Treichel
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002606:Charcoal / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006603:Hexuronic Acids / Q000737:chemistry; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007716:Kluyveromyces / Q000201:enzymology*
chemical_list: D000464:Alginates; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D006603:Hexuronic Acids; D002606:Charcoal; D020723:Glucuronic Acid; D006026:Glycoside Hydrolases; C023086:inulinase; D005976:Glutaral