title: The protective role of ascorbic acid (vitamin C) against chlorpyrifos-induced oxidative stress in Oreochromis niloticus.
abstract: Ability of ascorbic acid (vitamin C) to attenuate oxidative damage was evaluated in liver and brain tissues of Oreochromis niloticus (O. niloticus) experimentally exposed to sublethal concentrations of chlorpyrifos (CPF). O. niloticus was exposed to sublethal concentrations of CPF at 12 μg/L (CPF1) and 24 μg/L (CPF2) for 96 h. The fish of vitamin C (Vit C) and CPF2 + Vit C groups were fed with Vit C supplemented diet (200 mg Vit C/100 g feed). A significant increase in thiobarbituric acid-reactive substances (TBARS) level (P < 0.05) was observed in brain of CPF-exposed fish although liver TBARS level was not changed compared to control group. This result showed that lipid peroxidation (LPO) was elevated in brain of fish exposed to CPF. Glutathione peroxidase (GSH-Px) activity in liver and brain tissues was significantly elevated (P < 0.05) by exposure to CPF1 and CPF2. Catalase (CAT) activity was significantly increased (P < 0.05) in liver but decreased in brain of treated fish by CPF2 concentration. Superoxide dismutase (SOD) activity was decreased in liver, but increased in brain by exposure to CPF1 and CPF2 concentrations. Levels of TBARS were increased in brain of CPF-treated animals, but tended to decrease by the effect of Vit C. Vit C treatment for CPF-intoxicated animals normalized the otherwise raised activities of GSH-Px, CAT, and SOD within normal limits. The results clearly indicate that exposure to CPF caused a dose-dependent increase in oxidative stress brain and to a lesser extend in liver of fish and the ability of Vit C to attenuate CPF-induced oxidative damage.
authors.affiliation: Department of Basic Sciences, Faculty of Fisheries, Mersin University, Mersin, Turkey. ferbal1111@hotmail.com
authors.forename: Ferbal
authors.initials: F
authors.lastname: Ozkan
authors.forename: Suna Gül
authors.initials: SG
authors.lastname: Gündüz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Berköz
authors.forename: Arzu Ozlüer
authors.initials: AO
authors.lastname: Hunt
authors.forename: Serap
authors.initials: S
authors.lastname: Yalın
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000633:toxicity*; D023681:Cichlids / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity
chemical_list: D029941:Fish Proteins; D007306:Insecticides; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D004390:Chlorpyrifos; D001205:Ascorbic Acid