title: Depot-specific and hypercaloric diet-induced effects on the osteoblast and adipocyte differentiation potential of adipose-derived stromal cells.
abstract: Adipose-derived stromal cells (ADSCs) can be differentiated in vitro into several mesenchyme-derived cell types. We had previously described depot-specific differences in the adipocyte differentiation of ADSCs, and consequently we hypothesized that there may also be depot-specific differences in osteoblast differentiation of ADSCs. For this study, the osteoblast differentiation potential of rat subcutaneous ADSCs (scADSCs) and perirenal visceral ADSCs (pvADSCs) was compared. Osteoblast differentiation media (OM) induced markers of the osteoblastic phenotype in scADSCs, but not in pvADSCs. ADSCs harvested from rats with diet-induced visceral obesity (DIO) exhibited reduced osteoinduction, compared to lean controls, but adipocyte differentiation was not affected. Expression of the pro-osteogenic transcription factor Msx2 was significantly higher in naïve scADSCs from lean and DIO rats than in pvADSCs. Our findings indicate that ADSCs from different anatomical sites are uniquely pre-programmed in vivo in a depot-specific manner, and that diet-induced metabolic disturbances translate into reduced osteoblast differentiation of ADSCs.
authors.affiliation: Division of Endocrinology, Department of Medicine, Faculty of Health Sciences, University of Stellenbosch, Tygerberg Campus, P.O. Box 19063, Francie van Zijl Drive, Parow 7505, South Africa.
authors.forename: Hanel
authors.initials: H
authors.lastname: Sadie-Van Gijsen
authors.forename: Wayne
authors.initials: W
authors.lastname: Smith
authors.forename: Eugene Francois
authors.initials: EF
authors.lastname: du Toit
authors.forename: John
authors.initials: J
authors.lastname: Michie
authors.forename: F S
authors.initials: FS
authors.lastname: Hough
authors.forename: William Frank
authors.initials: WF
authors.lastname: Ferris
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000473:pathology*; D000273:Adipose Tissue / Q000473:pathology*; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000943:Antigens, Differentiation / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D002149:Energy Intake; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D008297:Male; D009765:Obesity; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D000943:Antigens, Differentiation; D050659:Core Binding Factor Alpha 1 Subunit; D018398:Homeodomain Proteins; D058574:Integrin-Binding Sialoprotein; C084119:MSX2 protein; C493717:Runx2 protein, rat; D000469:Alkaline Phosphatase