title: Receptor for advanced glycation end products (RAGE) prevents endothelial cell membrane resealing and regulates F-actin remodeling in a beta-catenin-dependent manner.
abstract: Receptor for advanced glycation end products (RAGE), an immunoglobin superfamily cell surface receptor, contributes to the vascular pathology associated with multiple disorders, including Alzheimer disease (AD), diabetic complications, and inflammatory conditions. However, the underlying mechanisms remain largely unclear. Here, using the human umbilical vein endothelial cell line (ECV-304) expressing human RAGE, we report that RAGE expression leads to an altered F-actin organization and impaired membrane resealing. To investigate the underlying mechanisms, we showed that RAGE expression increases β-catenin level, which decreases F-actin stress fibers and attenuates plasma membrane resealing. These results thus suggest a negative function for RAGE in endothelial cell membrane repair and reveal a new mechanism underlying RAGE regulation of F-actin remodeling and membrane resealing.
authors.affiliation: Institute of Molecular Medicine and Genetics and Department of Neurology, Georgia Health Sciences University, Augusta, Georgia 30912, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
authors.forename: Sergey
authors.initials: S
authors.lastname: Leonov
authors.forename: Amber Cyan
authors.initials: AC
authors.lastname: Howard
authors.forename: Shan
authors.initials: S
authors.lastname: Xiong
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Paul
authors.initials: P
authors.lastname: McNeil
authors.forename: Sylvia
authors.initials: S
authors.lastname: Simon
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Xiong
mesh_terms: D000199:Actins / Q000378:metabolism*; D000544:Alzheimer Disease / Q000378:metabolism; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology*; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods; D008954:Models, Biological; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014162:Transfection; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D051176:beta Catenin