title: Effects of variant rs346473 in ARHGAP24 gene on disease progression of HBV infection in Han Chinese population.
abstract: Host genetic, environmental and viral factors are classified as three categories that determine clinical outcomes of hepatitis B virus (HBV) infection. The objective of this study was to detect the associations between polymorphisms rs346473 and rs346482 in Rho GTPase-activating protein 24 (ARHGAP24) gene and disease progression of HBV infection in Han Chinese population. These two SNPs were found by our DNA pooling using Affymetrix Genome-Wide Human Mapping SNP6.0 Array in HBV carriers, and verified by using TaqMan 7900HT Sequence Detection System with 758 progressed HBV carriers versus 300 asymptomatic HBV carriers (AsC) in a discovery phase and 971 progressed HBV carriers versus 328 AsC in a replication phase. Multivariable logistic regression revealed that individuals with genotype TT at variant rs346473 displayed remarkable correlations with disease progression of HBV infection both in the discovery phase (OR, 2.693; 95% CI, 1.928-3.760; P=6.2×10(-9); additive model) and the replication phase (OR, 1.490; 95% CI, 1.104-2.012; P=9.0×10(-3); additive model). These two SNPs were in strong linkage disequilibrium with D'=0.99 and r (2)=0.951, and haplotype TT disclosed an increased susceptibility to HBV progression (OR, 1.980; 95% CI, 1.538-2.545; P=8.1×10(-8)). These findings suggest that polymorphism rs346473 in the ARHGAP24 gene might be a part of the genetic variants underlying the susceptibility of HBV carriers to disease progression.
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.|Liaocheng People's Hospital, Liaocheng, 252000, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jinjian
authors.initials: J
authors.lastname: Yao
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Liaocheng People's Hospital, Liaocheng, 252000, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Yu
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Sun
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Infection Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yongwen
authors.initials: Y
authors.lastname: He
authors.affiliation: Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. jslin@tjh.tjmu.edu.cn.
authors.forename: Jusheng
authors.initials: J
authors.lastname: Lin
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D020690:GTPase-Activating Proteins / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C497033:ARHGAP24 protein, human; D020690:GTPase-Activating Proteins