title: Terbium-sensitized fluorescence method for the determination of deferasirox in biological fluids and tablet formulation.
abstract: A novel, rapid and sensitive spectroflurimetric method was developed and validated for the determination of deferasirox in urine, serum and tablet samples based on sensitization of terbium fluorescence. The excitation and emission wavelengths were 328 and 545 nm, respectively. The optimum conditions for the determination of deferasirox were investigated considering the effects of various parameters. The method was quantitatively evaluated in terms of linearity, recovery, reproducibility and limit of detection. Under the optimal conditions, the fluorescence intensities were linear with the concentration of deferasirox in the range of 5 × 10(-9) to 5×10(-6) mol L(-1) , with a detection limit of 1.5 × 10(-9) mol L(-1) and a relative standard deviation of 1.1-2.3%. Linearity, reproducibility, recovery and limit of detection made the method suitable for determination of deferasirox in urine, serum and tablets samples.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran. manzoori@tabrizu.ac.ir
authors.forename: Jamshid L
authors.initials: JL
authors.lastname: Manzoori
authors.forename: Abolghasem
authors.initials: A
authors.lastname: Jouyban
authors.forename: Mohammad
authors.initials: M
authors.lastname: Amjadi
authors.forename: Vahid
authors.initials: V
authors.lastname: Panahi-Azar
authors.forename: Elnaz
authors.initials: E
authors.lastname: Tamizi
authors.forename: Jalil
authors.initials: J
authors.lastname: Vaez-Gharamaleki
mesh_terms: D001565:Benzoates / Q000032:analysis*; D000077588:Deferasirox; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry*; D013725:Terbium / Q000737:chemistry*; D014230:Triazoles / Q000032:analysis*
chemical_list: D001565:Benzoates; D013607:Tablets; D014230:Triazoles; D013725:Terbium; D000077588:Deferasirox