title: Comparison of antifungal activities of gentian violet and povidone-iodine against clinical isolates of Candida species and other yeasts: a framework to establish topical disinfectant activities.
abstract: We evaluated antifungal activity as assessed by the contact time in topical use of gentian violet (GV) and povidone-iodine (PI) against Candida strains. A total of 102 yeast isolates were used in this study. A markedly lower minimal inhibitory concentration (MIC)(90) of GV than of PI was detected for all yeast isolates. No remarkable difference in the MICs was observed among the identical strains isolated from different clinical sites for both GV and PI. Although the minimal fungicidal activities (MFCs) of PI were identical for all tested time points, the fungicidal activity of GV decreased during the time course of incubation. These results indicate that, whereas GV is more effective than PI, the topical disinfectant efficacy of GV should be estimated using the MFC(5 min) and not the MIC or the MFC(24 h) for overall prevention of catheter-related bloodstream infections and oral infections.
authors.affiliation: Department of Clinical Laboratory Medicine, School of Medicine, Juntendo University, Tokyo, Japan. metodo@mail.goo.ne.jp
authors.forename: Shigemi
authors.initials: S
authors.lastname: Kondo
authors.forename: Yoko
authors.initials: Y
authors.lastname: Tabe
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Yamada
authors.forename: Shigeki
authors.initials: S
authors.lastname: Misawa
authors.forename: Toyoko
authors.initials: T
authors.lastname: Oguri
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002177:Candidiasis / Q000382:microbiology; D005840:Gentian Violet / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D011206:Povidone-Iodine / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D011206:Povidone-Iodine; D005840:Gentian Violet