title: Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge.
abstract: Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 μg L(-1) Cd and 10, 20 μg L(-1) Cu but depressed by 10 μg L(-1) Cd and 40 μg L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum.
authors.affiliation: Key Laboratory of Coastal Zone Environment Processes, CAS/Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors.forename: Linbao
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Liu
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.forename: Liping
authors.initials: L
authors.lastname: You
authors.forename: Dong
authors.initials: D
authors.lastname: Pei
authors.forename: Ming
authors.initials: M
authors.lastname: Cong
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhao
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.forename: Dongyan
authors.initials: D
authors.lastname: Liu
authors.forename: Junbao
authors.initials: J
authors.lastname: Yu
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000633:toxicity; D049872:Bivalvia / Q000201:enzymology* / Q000276:immunology* / Q000382:microbiology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000276:immunology*; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism; D019216:Metals, Heavy / Q000633:toxicity; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014733:Vibrio / Q000276:immunology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D019216:Metals, Heavy; D012333:RNA, Messenger; D001564:Benzo(a)pyrene; D005979:Glutathione Peroxidase