title: Prevention of influenza virus shedding and protection from lethal H1N1 challenge using a consensus 2009 H1N1 HA and NA adenovirus vector vaccine.
abstract: Vaccines against emerging pathogens such as the 2009 H1N1 pandemic virus can benefit from current technologies such as rapid genomic sequencing to construct the most biologically relevant vaccine. A novel platform (Ad5 [E1-, E2b-]) has been utilized to induce immune responses to various antigenic targets. We employed this vector platform to express hemagglutinin (HA) and neuraminidase (NA) genes from 2009 H1N1 pandemic viruses. Inserts were consensuses sequences designed from viral isolate sequences and the vaccine was rapidly constructed and produced. Vaccination induced H1N1 immune responses in mice, which afforded protection from lethal virus challenge. In ferrets, vaccination protected from disease development and significantly reduced viral titers in nasal washes. H1N1 cell mediated immunity as well as antibody induction correlated with the prevention of disease symptoms and reduction of virus replication. The Ad5 [E1-, E2b-] should be evaluated for the rapid development of effective vaccines against infectious diseases.
authors.affiliation: Etubics Corporation, Seattle, WA 98199, USA.
authors.forename: Frank R
authors.initials: FR
authors.lastname: Jones
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Gabitzsch
authors.forename: Younong
authors.initials: Y
authors.lastname: Xu
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Balint
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Borisevich
authors.forename: Jennifer
authors.initials: J
authors.lastname: Smith
authors.forename: Jeanon
authors.initials: J
authors.lastname: Smith
authors.forename: Bi-Hung
authors.initials: BH
authors.lastname: Peng
authors.forename: Aida
authors.initials: A
authors.lastname: Walker
authors.forename: Magda
authors.initials: M
authors.lastname: Salazar
authors.forename: Slobodan
authors.initials: S
authors.lastname: Paessler
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology; D032921:Consensus; D005289:Ferrets; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D007251:Influenza, Human / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D051379:Mice; D017817:Nasal Lavage Fluid / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination / Q000379:methods; D014779:Virus Replication / Q000235:genetics / Q000276:immunology; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D006388:Hemagglutinins; D007252:Influenza Vaccines; D009439:Neuraminidase