title: Encapsulation of docetaxel into PEGylated gold nanoparticles for vectorization to cancer cells.
abstract: Encapsulation of docetaxel and its solubilization in water was carried out in PEGylated gold nanoparticles (AuNPs) as shown by 1H NMR (600 MHz) and UV/Vis spectroscopy and dynamic light scattering. Vectorization of PEGylated AuNP-encapsulated docetaxel was probed in vitro toward human colon carcinoma (HCT15) and human breast cancer (MCF7) cells. AuNPs alone presented no cytotoxicity toward either MCF7 or HCT15 adenocarcinoma cells. AuNP-docetaxel was found to be 2.5-fold more efficient than docetaxel alone against MCF7 cells, and the IC50 value of AuNP-docetaxel against HCT15 cells was lower than that of free docetaxel; the increased efficiency brought about by AuNP drug encapsulation was ∼1.5-fold.
authors.affiliation: Institut des Sciences Moléculaires, UMR CNRS 5255, Université Bordeaux I, 351 Cours de la Libération, 33405 Talence Cedex, France.
authors.forename: Alison
authors.initials: A
authors.lastname: François
authors.forename: Audrey
authors.initials: A
authors.lastname: Laroche
authors.forename: Noël
authors.initials: N
authors.lastname: Pinaud
authors.forename: Lionel
authors.initials: L
authors.lastname: Salmon
authors.forename: Jaime
authors.initials: J
authors.lastname: Ruiz
authors.forename: Jacques
authors.initials: J
authors.lastname: Robert
authors.forename: Didier
authors.initials: D
authors.lastname: Astruc
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry*; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000188:drug therapy; D000077143:Docetaxel; D004337:Drug Carriers; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D043823:Taxoids / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D043823:Taxoids; D000077143:Docetaxel; D011092:Polyethylene Glycols; D006046:Gold