title: Prevalence of human papillomavirus genotypes and associated cervical squamous intraepithelial lesions in HIV-infected women in Botswana.
abstract: Human papillomaviruses (HPV) constitute one of the most prevalent sexually transmitted infections and are the etiological agents for invasive cervical cancer, the predominant cancer among women in Botswana. However, the prevalence of HPV genotypes in Botswana has yet to be reported. One hundred thirty-nine endocervical swabs were taken at baseline from HIV-1 infected, HSV-2 seropositive women enrolled in a longitudinal cohort study designed to assess the influence of herpes simplex virus-2 (HSV-2) infection on genital tract shedding of HIV-1. Extracted DNA was evaluated for the presence of low-risk and high-risk HPV using the Roche Linear Array. Genotyping identified HPV in 95 of 139 women of which 61/95 were infected with high-risk HPV and 56/95 with low-risk HPV. The median number of genotypes was 2 (IQR: 1-4). The most prevalent HPV genotype in HIV-infected women was HPV 58. Abnormal cervical cytology was detected in 87/127 women and was associated with contemporaneous HPV infection (RR = 1.43, 95% CI: 1.05-1.93; P = 0.02). HPV prevalence was high among HIV-infected women with infection by multiple genotypes being widespread. The associations attributed to specific oncogenic HPV subtypes and cervical squamous intraepithelial lesions presented here provide critical information to inform future vaccine policy within Botswana.
authors.affiliation: Harvard School of Public Health AIDS Initiative, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA.
authors.forename: Iain J
authors.initials: IJ
authors.lastname: Macleod
authors.forename: Belinda
authors.initials: B
authors.lastname: O'Donnell
authors.forename: Sikhulile
authors.initials: S
authors.lastname: Moyo
authors.forename: Shahin
authors.initials: S
authors.lastname: Lockman
authors.forename: Roger L
authors.initials: RL
authors.lastname: Shapiro
authors.forename: Mukendi
authors.initials: M
authors.lastname: Kayembe
authors.forename: Erik
authors.initials: E
authors.lastname: van Widenfelt
authors.forename: Joseph
authors.initials: J
authors.lastname: Makhema
authors.forename: M
authors.initials: M
authors.lastname: Essex
authors.forename: Carolyn
authors.initials: C
authors.lastname: Wester
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics*; D001902:Botswana / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D006558:Herpes Genitalis / Q000150:complications; D018258:Herpesvirus 2, Human / Q000276:immunology; D006801:Humans; D008137:Longitudinal Studies; D030361:Papillomavirus Infections / Q000150:complications* / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral