title: Crystallization and preliminary X-ray diffraction studies of BmooPLA2-I, a platelet-aggregation inhibitor and hypotensive phospholipase A2 from Bothrops moojeni venom.
abstract: Phospholipases A(2) (PLA(2)s) are enzymes that cause the liberation of fatty acids and lysophospholipids by the hydrolysis of membrane phospholipids. In addition to their catalytic action, a wide variety of pharmacological activities have been described for snake-venom PLA(2)s. BmooPLA(2)-I is an acidic, nontoxic and catalytic PLA(2) isolated from Bothrops moojeni snake venom which exhibits an inhibitory effect on platelet aggregation, an immediate decrease in blood pressure, inducing oedema at a low concentration, and an effective bactericidal effect. BmooPLA(2)-I has been crystallized and X-ray diffraction data have been collected to 1.6 Å resolution using a synchrotron-radiation source. The crystals belonged to space group C222(1), with unit-cell parameters a = 39.7, b = 53.2, c = 89.2 Å. The molecular-replacement solution of BmooPLA(2)-I indicated a monomeric conformation, which is in agreement with nondenaturing electrophoresis and dynamic light-scattering experiments. A comparative study of this enzyme with the acidic PLA(2) from B. jararacussu (BthA-I) and other toxic and nontoxic PLA(2)s may provide important insights into the functional aspects of this class of proteins.
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP-Universidade Estadual Paulista, Botucatu-SP, Brazil.
authors.forename: Guilherme H M
authors.initials: GH
authors.lastname: Salvador
authors.forename: Daniela P
authors.initials: DP
authors.lastname: Marchi-Salvador
authors.forename: Lucas B
authors.initials: LB
authors.lastname: Silveira
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
mesh_terms: D000818:Animals; D017837:Bothrops; D003460:Crystallization; D018360:Crystallography, X-Ray; D054467:Phospholipases A2 / Q000737:chemistry*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry*; D012910:Snake Venoms / Q000737:chemistry*
chemical_list: D010975:Platelet Aggregation Inhibitors; D012910:Snake Venoms; D054467:Phospholipases A2