title: LC-MS/MS method for the quantification of myo- and chiro-inositol as the urinary biomarkers of insulin resistance in human urine.
abstract: A simple LC-MS/MS method has been developed and validated for the quantification of endogenous myo- and chiro-inositol in human urine. myo- and chiro-Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient (n = 3) was greater than 0.9991 over the range 0.05-25.0 µg/mL with the weighted (1/C²) least square method. Precision (%RSD) and accuracy (%RE) were 0-10.0% and 0-6.0% for the intra-day assay (n = 5) and 0-14.3% and 0-10.0% for the inter-day assay (n = 5). myo- and chiro-Inositol have been shown to be stable in human urine stored at room temperature and for three freeze-thaw cycles.
authors.affiliation: Analytical Science, LG Chemical Research Park, LG Chemicals Ltd, 104-1 Moonji-Dong, Yusong-Gu, Daejeon 305-380, South Korea.
authors.forename: Byoung-Hyoun
authors.initials: BH
authors.lastname: Kim
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Park
authors.forename: Jae Bum
authors.initials: JB
authors.lastname: Jang
authors.forename: Dong Cheul
authors.initials: DC
authors.lastname: Moon
mesh_terms: D015415:Biomarkers / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D007294:Inositol / Q000652:urine*; D007333:Insulin Resistance; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D007294:Inositol