title: Bloodstream and non-invasive isolates of Candida glabrata have similar population structures and fluconazole susceptibilities.
abstract: We have compared multilocus sequence typing (MLST) and fluconazole susceptibility profiles of Candida glabrata bloodstream isolates obtained during active, population-based surveillance to those obtained from non-sterile sites of individuals with no evidence of fungal disease (i.e., non-invasive isolates) in the same US city during an overlapping time period. In each of the two populations, different proportions of the same six major sequence types (STs) encompassed 82% of the isolates. One ST was more prevalent in the candidemia population and two other STs were more prevalent in the non-invasive population, but the overall allelic frequencies within the groups suggested little, if any, genotypic diversity between them. Fluconazole susceptibility profiles of isolates from the patients in the two groups were not significantly different and were not associated with a particular sequence type. Our results support the hypothesis that C. glabrata strains causing bloodstream infections are genetically indistinguishable from those normally residing in/on the host, suggesting that relative pathogenicity may be closely tied to commensalism.
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, Mailstop G-11, Atlanta, GA 30333, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Lott
authors.forename: Joao P
authors.initials: JP
authors.lastname: Frade
authors.forename: G Marshall
authors.initials: GM
authors.lastname: Lyon
authors.forename: Naureen
authors.initials: N
authors.lastname: Iqbal
authors.forename: Shawn R
authors.initials: SR
authors.lastname: Lockhart
mesh_terms: D000704:Analysis of Variance; D000935:Antifungal Agents / Q000494:pharmacology*; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D058387:Candidemia / Q000382:microbiology*; D002177:Candidiasis / Q000382:microbiology*; D025141:Drug Resistance, Fungal; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D025341:Principal Component Analysis
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole