title: [Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines].
abstract: To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines.
  
  
  
  Using human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.
  
  
  
  The splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed.
  
  
  
  NCAM L1 minigene model can be used in alternative splicing analysis.
authors.affiliation: School of Medicine and Life Science, Zhejiang University City College, Hangzhou 310015, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Quan
authors.initials: Q
authors.lastname: Shen
authors.forename: Zheng-yu
authors.initials: ZY
authors.lastname: Peng
mesh_terms: D002460:Cell Line; D005822:Genetic Vectors; D006801:Humans; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012326:RNA Splicing; D014162:Transfection
chemical_list: D039842:Neural Cell Adhesion Molecule L1