title: Gold nano-popcorn attached SWCNT hybrid nanomaterial for targeted diagnosis and photothermal therapy of human breast cancer cells.
abstract: Breast cancer presents greatest challenge in health care in today's world. The key to ultimately successful treatment of breast cancer disease is an early and accurate diagnosis. Current breast cancer treatments are often associated with severe side effects. Driven by the need, we report the design of novel hybrid nanomaterial using gold nano popcorn-attached single wall carbon nanotube for targeted diagnosis and selective photothermal treatment. Targeted SK-BR-3 human breast cancer cell sensing have been performed in 10 cancer cells/mL level, using surface enhanced Raman scattering of single walls carbon nanotube's D and G bands. Our data show that S6 aptamer attached hybrid nanomaterial based SERS assay is highly sensitive to targeted human breast cancer SK-BR-3 cell line and it will be able to distinguish it from other non targeted MDA-MB breast cancer cell line and HaCaT normal skin cell line. Our results also show that 10 min of photothermal therapy treatment by 1.5 W/cm(2) power, 785 nm laser is enough to kill cancer cells very effectively using S6 aptamer attached hybrid nanomaterials. Possible mechanisms for targeted sensing and operating principle for highly efficient photothermal therapy have been discussed. Our experimental results reported here open up a new possibility for using aptamers modified hybrid nanomaterial for reliable diagnosis and targeted therapy of cancer cell lines quickly.
authors.affiliation: Department of Chemistry, Jackson State University, Jackson, Mississippi, United States.
authors.forename: Lule
authors.initials: L
authors.lastname: Beqa
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fan
authors.forename: Anant Kumar
authors.initials: AK
authors.lastname: Singh
authors.forename: Dulal
authors.initials: D
authors.lastname: Senapati
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D001943:Breast Neoplasms / Q000175:diagnosis / Q000628:therapy*; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D005260:Female; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010789:Phototherapy; D013059:Spectrum Analysis, Raman
chemical_list: D052157:Aptamers, Nucleotide; D002232:Carbocyanines; D037742:Nanotubes, Carbon; C089187:cyanine dye 3; D006046:Gold