title: Transcriptional profiling of host gene expression in chicken liver tissues infected with oncogenic Marek's disease virus.
abstract: Marek's disease virus (MDV), one of the most potent oncogenic herpesviruses, leads to highly contagious immunosuppressive and neoplastic disease in susceptible chickens. Previous studies mainly focused on the roles of host genes modulated by MDV in the virological rather than the neoplastic stage of disease. To investigate the molecular mechanisms of tumorigenesis in Marek's disease further, a microarray analysis with Affymetrix Gene-Chip Chicken Genome Arrays was performed in a non-lymphoid tissue liver during the neoplastic stage. Of the 32 773 chicken transcriptions arrayed on a chip, 269 genes were significantly differentially expressed during the neoplastic stage caused by MDV infection (upregulated, 175; downregulated, 94). The altered genomic expression of 15 randomly selected genes was confirmed by real-time RT-PCR. Biological functions and pathways of the group of 269 differentially expressed genes were analysed by using a bioinformatics tool (ipa, Ingenuity Pathway Analysis). The results revealed that 19 possible gene networks with intermolecular connections and 22 significant metabolic and signalling pathways (P≤0.05) among 137 differentially expressed genes. These 137 genes were classified into a number of functional groups that included genetic disorder, cancer, cellular growth and proliferation, and cell death. In summary, the investigation of global host-gene expression, providing the biological functions of differentially expressed genes in lymphoid tumours of the liver in response to MDV infections, may contribute to a basic understanding of the molecular mechanisms involved in tumorigenesis following MDV infection.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Cuiying
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Xie
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Shang
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Siwei
authors.initials: S
authors.lastname: Bai
authors.affiliation: State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China.
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Animal and Food Science, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Yingzuo
authors.initials: Y
authors.lastname: Bi
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000821:virology; D019295:Computational Biology; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation; D008381:Herpesvirus 2, Gallid / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D008099:Liver / Q000473:pathology / Q000821:virology; D008380:Marek Disease / Q000235:genetics* / Q000473:pathology / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis; D009858:Oncogenic Viruses / Q000472:pathogenicity*; D060888:Real-Time Polymerase Chain Reaction