title: Construction of multidrug-sensitive yeast with high sporulation efficiency.
abstract: Budding yeast is often used in chemical genetics for screening, target identification, and compound verification, but its high-level drug resistance has made the analysis of compounds difficult. Here we report the construction of 12geneΔ0HSR, a strain that lacks eight efflux pumps located on the plasma membrane and four transcription factors involved in expression of efflux pumps, and contains the RME1(ins-308A) mutation. This strain retained sufficient transformation, mating, and sporulation efficiency for genetic analysis in addition to hypersensitivity against several compounds. 12geneΔ0HSR is a useful tool for chemical biology, not only in chemical screening but in target identification and verification of bioactive compounds.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennodai, Tsukuba, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Chinen
authors.forename: Yu
authors.initials: Y
authors.lastname: Ota
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nagumo
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Masumoto
authors.forename: Takeo
authors.initials: T
authors.lastname: Usui
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000172:deficiency* / Q000235:genetics; D009154:Mutation; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000172:deficiency* / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D000935:Antifungal Agents; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D014157:Transcription Factors