title: Controlling the surface properties of nanostructures for studies of polymerases.
abstract: We report the successful functionalization of optically accessible nanostructures, suitable for single-molecule experiments at physiological substrate concentrations, with polyethylene glycol. Characterization of the coating in terms of roughness, protein repellence, and specific immobilization of DNA is described. We present an application of this technique in the detection of polymerase activity within nanostructures, which demonstrates the opportunities made possible through the integration of nanofabricated structures with surface functionalization.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Crut
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Koster
authors.forename: Zhuangxiong
authors.initials: Z
authors.lastname: Huang
authors.forename: Susanne
authors.initials: S
authors.lastname: Hage
authors.forename: Nynke H
authors.initials: NH
authors.lastname: Dekker