title: Crystallization and X-ray diffraction analysis of nylon-oligomer hydrolase (NylC) from Agromyces sp. KY5R.
abstract: 6-Aminohexanoate-oligomer hydrolase (NylC) from Agromyces sp. KY5R was expressed in Escherichia coli JM109 and purified by ammonium sulfate fractionation, anion-exchange column chromatography and gel-filtration chromatography. NylC was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as a precipitant in 0.1 M HEPES buffer pH 7.5 containing 0.2 M NaCl. Diffraction data were collected from native and K(2)PtCl(4)-derivative crystals to resolutions of 2.00 and 2.20 Å, respectively. The obtained crystal was plate-shaped, with an I-centred orthorhombic space group and unit-cell parameters a = 155.86, b = 214.45, c = 478.80 Å. The anomalous difference Patterson map of the K(2)PtCl(4)-derivative crystal suggested that the space group was I222 rather than I2(1)2(1)2(1).
authors.affiliation: Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, Himeji, Hyogo, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Yasuhira
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Naoya
authors.initials: N
authors.lastname: Kumagai
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagai
authors.forename: Dai-ichiro
authors.initials: D
authors.lastname: Kato
authors.forename: Masahiro
authors.initials: M
authors.lastname: Takeo
authors.forename: Seiji
authors.initials: S
authors.lastname: Negoro
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D009757:Nylons / Q000378:metabolism
chemical_list: D009757:Nylons; D000581:Amidohydrolases