title: [Study on the toxic reaction induced by single dose of qingkailing injection].
abstract: OBJECTIVE
  To study the toxic reaction induced by Qingkailing Injection.
  
  
  METHODS
  Kunming mice were injected single dose of Qingkailing Injection via tail vein and observed for 14 days to test the toxic reaction of the drug. According to Chinese Pharmacopoeia, hemolysis tests were conducted on the injections of different lots and each ingredient of the injection.
  
  
  RESULTS
  Toxic reaction induced by single dose of injection--with dose increasing,mice quickly showed different responses such as hypodynamia, convulsion, syncope and even death after injection. In the high dose group, blood routine detection showed that mice have lower counts of RBC, WBC and lower content of hemoglobin; The pulmonary pathological sections of dead mice showed significant hyperemia. And there were no significant difference in the contents of serum electrolyte (K+, Na+, Ca2+) between normal saline control group and Qingkailing injection group. Hemolysis test in vitro--Honeysuckle extraction (significantly) and gardenia extraction which were components of Qing-kailing injection caused hemolysis in certain dose; While gardenia, pearl shell and isatis root extraction caused RBC agglutination. With higher concentration, the Qingkailing injections of different lot caused different degree of hemolysis. There was no significant difference in the hemolysis test in vitro between the group of Balb/C mice which were sensitized by Qingkailing injection or not.
  
  
  CONCLUSIONS
  In clinical practice some adverse reactions induced by Qingkailing injection occurred concomitantly with acute hemolysis within vessels, which might be caused by honeysuckle and gardenia. And the hemolysis was independent of allergy.
authors.affiliation: Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. lijing-c@hotmail.com
authors.forename: Li-jing
authors.initials: LJ
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shuai
authors.initials: S
authors.lastname: He
authors.forename: Guo-ping
authors.initials: GP
authors.lastname: Liao
authors.forename: Zhong-yi
authors.initials: ZY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000009:adverse effects* / Q000737:chemistry; D004573:Electrolytes / Q000097:blood; D004903:Erythrocyte Aggregation / Q000187:drug effects; D005260:Female; D032062:Gardenia / Q000009:adverse effects / Q000737:chemistry; D006461:Hemolysis / Q000187:drug effects*; D006462:Hemolytic Plaque Technique; D007267:Injections; D031239:Lonicera / Q000009:adverse effects / Q000737:chemistry; D008168:Lung / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D011817:Rabbits; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D020323:Tics / Q000139:chemically induced*; D018675:Toxicity Tests
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D004573:Electrolytes; C504928:Qingkailing