title: [Effects and mechanism of Plastrum testudinis extracts on PC12 apoptosis].
abstract: OBJECTIVE
  To observe the inhibitive effects of Plastrum testudinis Extracts (PTE) on 6-Hydroxydopamine (6-OHDA) induced PC12 cells apoptosis and explore its mechanism.
  
  
  METHODS
  PC12 apoptosis model was established by serum starvation and damaged for 24 hours. The cells were randomly divided into four groups:control group, 6-OHDA group, PTE 3, 30 microg/mL group. Cell optical density was determined by MTT; Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the BCL-X/L expression.
  
  
  RESULTS
  MTT and FCM analysis demonstrated that PTE can elevate PC12 cells viability and reduce their apoptotic ratio in a dose dependent manner. Western blot showed that PTE promoted the expression of BCL-X/L.
  
  
  CONCLUSIONS
  PTE can inhibit the apoptosis of PC12 induced by 6-OHDA in a dose dependent manner, and its mechanism maybe associated partially with up-regulating BCL-X/L signaling pathway.
authors.affiliation: Department of Anatomy, Preclinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Wu
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Cao
authors.forename: Dong-Feng
authors.initials: DF
authors.lastname: Chen
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Zhou
authors.forename: Ru-Dong
authors.initials: RD
authors.lastname: Deng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D008419:Materia Medica / Q000008:administration & dosage / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D016627:Oxidopamine / Q000009:adverse effects; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D014426:Turtles; D015854:Up-Regulation / Q000187:drug effects; D051020:bcl-X Protein / Q000378:metabolism*
chemical_list: D008419:Materia Medica; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D016627:Oxidopamine