title: [Study on transport characteristics of honokiol in Caco-2 cell model by HPLC].
abstract: OBJECTIVE
  To study the transport mechanism of honokiol in Caco-2 cell model.
  
  
  METHODS
  The analysis was performed on a Kromasil 100-5 C18 column (4.5 mm x 250 mm, 5 microm) eluted with acetonitrile-water (70: 30) as mobile phase. The detection wavelength was set at 203 nm. Two-way transport of honokiol was studied by using Caco-2 cell model, and the effects of time, drug concentration, inhibitor, pH, temperature on the transport of honokiol was investigated. The drug concentrations were determined by high performance liquid chromatography(HPLC) and used to calculate the apparent permeability coefficient.
  
  
  RESULTS
  The standard curve of honokiol was Y = 24 044X - 3 763.6 (r = 0.999 8), and the detection limit was 0.04 micromol x L(-1). In Caco-2 cell model, the transport amounts from the top side to the base side of were more than that from the base side to the top side under the same concentration. The transport amounts increased with time both in AP --> BL and BL --> AP directions. Verapamil could improve the transport amounts of AP --> BL. There were no effects of pH on the transport of AP --> BL. Both in AP --> BL and BL --> AP directions, the transport showed temperature dependence.
  
  
  CONCLUSIONS
  Honokiol is transported through the intestinal mucosa via a passive diffusion mechanism primarily, coexisting with a carrier-mediated transport, at the same time effected by P-gp.
authors.affiliation: Research Institute of Mathematical Engineering of Guangzhou University of Chinese Medicine in Dongguan, Dongguan 523808, China. zb-101@tom.com
authors.forename: Bao
authors.initials: B
authors.lastname: Zeng
authors.forename: Anguo
authors.initials: A
authors.lastname: Wu
authors.forename: Jiannan
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaping
authors.initials: X
authors.lastname: Lai
mesh_terms: D001692:Biological Transport; D001713:Biphenyl Compounds / Q000032:analysis* / Q000493:pharmacokinetics*; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004058:Diffusion; D006801:Humans; D017705:Lignans / Q000032:analysis* / Q000493:pharmacokinetics*; D010539:Permeability; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols