title: High sensitivity molecular detection with enzyme-linked immuno-sorbent assay (ELISA)-type immunosensing.
abstract: Here we describe an immunosensing method, which is designed for high sensitivity sensing of various substances utilizing specificity of antigen-antibody (ELISA-type) interaction. The building up of the nanostructured sensing interface and the immunointeraction at the surface were characterized by atomic force microscopy. The proposed design makes potentially feasible attaining ultimate single-molecule sensitivity upon optimization of the system. The first non-optimized prototype described here has already demonstrated sensitivity to the presence of dinitrophenyl (DNP) in concentrations as low as 10 pM, which is 100 times better than reported limits of detection of DNP with a traditional enzyme-linked immuno-sorbent assay setup.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699, USA.
authors.forename: Marcos
authors.initials: M
authors.lastname: Pita
authors.forename: Lili
authors.initials: L
authors.lastname: Cui
authors.forename: Ravi M
authors.initials: RM
authors.lastname: Gaikwad
authors.forename: Evgeny
authors.initials: E
authors.lastname: Katz
authors.forename: Igor
authors.initials: I
authors.lastname: Sokolov