title: Attachment of HeLa cells during early G1 phase.
abstract: Both growth factor directed and integrin dependent signal transduction were shown to take place directly after completion of mitosis. The local activation of these signal transduction cascades was investigated in early G1 cells. Interestingly, various key signal transduction proteins were found in blebs at the cell membrane within 30 min after mitosis. These membrane blebs appeared in round, mitotic-like cells and disappeared rapidly during spreading of the cells in G1 phase. In addition to tyrosine-phosphorylated proteins, the blebs contained also phosphorylated FAK and phosphorylated MAP kinase. The formation of membrane blebs in round, mitotic cells before cell spreading is not specific for mitotic cells, because similar features were observed in trypsinized cells. Just before cell spreading also these cells exhibited membrane blebs containing active signal transduction proteins. Inhibition of signal transduction did not affect membrane bleb formation, suggesting that the membrane blebs were formed independent of signal transduction.
authors.affiliation: Department of Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands.
authors.forename: Maarten J A
authors.initials: MJ
authors.lastname: Moes
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Bijvelt
authors.forename: Johannes
authors.initials: J
authors.lastname: Boonstra
mesh_terms: D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008938:Mitosis; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D051416:Focal Adhesion Protein-Tyrosine Kinases; D020928:Mitogen-Activated Protein Kinases