title: [HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats].
abstract: OBJECTIVE
  To analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.
  
  
  METHODS
  Rat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.
  
  
  RESULTS
  In rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.
  
  
  CONCLUSIONS
  The methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.
authors.affiliation: Pony Testing Technology Co., Ltd., Beijing 100080, China. yanlizhi601@163.com
authors.forename: Lizhi
authors.initials: L
authors.lastname: Yan
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Qirong
authors.initials: Q
authors.lastname: Yu
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism*; D032064:Hedyotis / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal