title: Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1.
abstract: Heading date in rice is an important agronomic trait controlled by several genes. In this study, flowering time of variety Dianjingyou 1 (DJY1) was earlier than a near-isogenic line (named NIL) carried chromosome segment from African rice on chromosome 3S, when grown in both long-day (LD) and short-day (SD) conditions. By analyzing a large F2 population from NIL × DJY1, the locus DTH3 (QTL for days to heading on chromosome 3) controlling early heading date in DJY1 was fine mapped to a 64-kb segment which contained only one annotated gene, a MIKC-type MADS-box protein. We detected a 6-bp deletion and a single base substitution in the C-domain by sequencing DTH3 in DJY1 compared with dth3 in NIL, and overexpression of DTH3 caused early flowering in callus. Quantitative real-time PCR revealed that the transcript level of dth3 in NIL was lower than that DTH3 in DJY1 in both LD and SD conditions. The Early heading date 1 (Ehd1) which promotes the RFT1, was up-regulated by DTH3 in both LD and SD conditions. Based on Indel and dCAPs marker analysis, the dth3 allele was only present in African rice accessions. A phylogenetic analysis based on microsatellite genotyping suggested that African rice had a close genetic relationship to O. rufipogon and O. latifolia, and was similar to japonica cultivars. DTH3 affected flowering time and had no significant effect on the main agronomic traits.
authors.affiliation: National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Bian
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhao
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Gao
authors.forename: Y H
authors.initials: YH
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Zheng
authors.forename: L M
authors.initials: LM
authors.lastname: Chen
authors.forename: S J
authors.initials: SJ
authors.lastname: Liu
authors.forename: H Q
authors.initials: HQ
authors.lastname: Zhai
authors.forename: J M
authors.initials: JM
authors.lastname: Wan
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D015536:Down-Regulation; D035264:Flowers / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D017440:Photoperiod; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040641:Quantitative Trait Loci
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins