title: LC determination and pharmacokinetic study of vitexin-4″-O-glucoside in rat plasma after oral administration.
abstract: A simple and specific high-performance liquid chromatography (HPLC) method was developed for the pharmacokinetic study of vitexin-4″-O-glucoside (VOG) in rats after oral administration. The plasma samples were deproteinised with methanol after the addition of an internal standard, hesperidin. HPLC analysis was performed on a Diamonsil C(18) analytical column, using methanol -0.5% aqueous phosphoric acid (45:55, v/v) as the mobile phase with ultraviolet detection at 330 nm. The calibration curve was linear over the range of 5-450 µg mL(-1) in rat plasma. The average extraction recovery of VOG was 98.74% ± 0.44%, and the relative standard deviations of the intra- and inter-day precisions were not greater than 4.1% and 2.0%, respectively. The validated method was successfully applied during a pharmacokinetic study in rats after oral administration of VOG at different doses, and all the results indicated that the pharmacokinetics of VOG in rats obeyed nonlinear processes.
authors.affiliation: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, 116600, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.forename: Xixiang
authors.initials: X
authors.lastname: Ying
authors.forename: Tingguo
authors.initials: T
authors.lastname: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000097:blood / Q000493:pharmacokinetics*; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005960:Glucosides; D007529:Isoflavones; C477953:isovitexin-4'-O-glucoside