title: Fluorescent assessment of intracellular calcium in individual cells.
abstract: Calcium is an important intracellular ion involved in numerous cell processes. There are multiple factors that contribute to the release of Ca²⁺. Some factors induce release as part of intracellular signaling cascades, while others result in unwanted changes to both basal and inducible Ca²⁺ levels. The accurate measurement of intracellular Ca²⁺ is, therefore, an important tool in neurotoxicology for assessing compounds/substances that disrupt Ca²⁺ homeostasis. Fluorescent, Ca²⁺-sensitive probes Indo-1 and Fluo-3 allow for the quantification of intracellular Ca²⁺ in individual cells to determine what effects neurotoxins have on both basal and stimulus-dependent Ca²⁺ concentrations.
authors.affiliation: Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
authors.forename: David P
authors.initials: DP
authors.lastname: Cox
authors.forename: Collin C
authors.initials: CC
authors.lastname: White
authors.forename: Terrance J
authors.initials: TJ
authors.lastname: Kavanagh
mesh_terms: D000814:Aniline Compounds; D002118:Calcium / Q000737:chemistry / Q000378:metabolism*; D002138:Calibration; D045744:Cell Line, Tumor; D003627:Data Interpretation, Statistical; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D018613:Microscopy, Confocal / Q000379:methods; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D059010:Single-Cell Analysis; D013050:Spectrometry, Fluorescence; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D007211:Indoles; D014966:Xanthenes; C059587:Indo-1 pentaacetoxymethyl ester; C059715:Fluo-3; D002118:Calcium