title: Analyzing multiclonality of Staphylococcus aureus in clinical diagnostics using spa-based denaturing gradient gel electrophoresis.
abstract: We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.
authors.affiliation: Department of Laboratory Medicine, Clinical Microbiology, Ryhov County Hospital, Jönköping, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Matussek
authors.forename: Lisa
authors.initials: L
authors.lastname: Stark
authors.forename: Olaf
authors.initials: O
authors.lastname: Dienus
authors.forename: Joakim
authors.initials: J
authors.lastname: Aronsson
authors.forename: Sara
authors.initials: S
authors.lastname: Mernelius
authors.forename: Sture
authors.initials: S
authors.lastname: Löfgren
authors.forename: Per-Eric
authors.initials: PE
authors.lastname: Lindgren
mesh_terms: D060085:Coinfection / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis / Q000379:methods*; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D013205:Staphylococcal Protein A