title: Characterization of the complex of human-like collagen with calcium.
abstract: The calcium complex of human-like collagen (HLC) was generated in aqueous solution, purified by dialysis or ultrafiltration, and isolated by freeze-drying. Evidence for interactions between HLC and calcium(II) was obtained through measurements of the complex's fluorescence and UV-visible spectra, the interpretation of its Fourier transform infrared spectroscopy and circular dichroism spectra, thermogravimetric (TG) analysis, and differential scanning calorimetry (DSC). The HLC-Ca complex is considered a new chemical compound in which HLC retains its helical structure and the calcium ion is linked to the protein via C=O and N-H groups. Additionally, TG and DSC measurements indicate that the HLC-Ca complex is thermodynamically more stable than free HLC. The results obtained provide important evidence for the further study of the interactions of HLC with Ca(II).
authors.affiliation: Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 710069 Xi'an, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yu
authors.forename: Daidi
authors.initials: D
authors.lastname: Fan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D005612:Freeze Drying; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D012996:Solutions / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D013818:Thermogravimetry
chemical_list: D001672:Biocompatible Materials; D009942:Organometallic Compounds; D012996:Solutions; D003094:Collagen; D002118:Calcium