title: Crystal structure of the mismatch-specific thymine glycosylase domain of human methyl-CpG-binding protein MBD4.
abstract: Methyl-CpG (mCpG) binding domain protein 4 (MBD4) is a member of mammalian DNA glycosylase superfamily. It contains an amino-proximal methyl-CpG binding domain (MBD) and a C-terminal mismatch-specific glycosylase domain, which is an important molecule believed to be involved in maintaining of genome stability. Herein, we determined the crystal structure of C-terminal glycosylase domain of human MBD4. And the structural alignments of other helix-hairpin-helix (HhH) DNA glycosylases show that the human MBD4 glycosylase domain has the similar active site and the catalytic mechanisms as others. But the different residues in the N-terminal of domain result in the change of charge distribution on the surface of the protein, which suggest the different roles that may relate some diseases.
authors.affiliation: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Huazhong Normal University, Wuhan 430079, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhonglai
authors.initials: Z
authors.lastname: Liu
authors.forename: Lissete
authors.initials: L
authors.lastname: Crombet
authors.forename: Maria F
authors.initials: MF
authors.lastname: Amaya
authors.forename: Yanli
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiaoru
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenhua
authors.initials: W
authors.lastname: Kuang
authors.forename: Pengtao
authors.initials: P
authors.lastname: Ma
authors.forename: Liping
authors.initials: L
authors.lastname: Niu
authors.forename: Chao
authors.initials: C
authors.lastname: Qi
mesh_terms: D000595:Amino Acid Sequence; D020137:Base Pair Mismatch; D002384:Catalysis; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D044502:Thymine DNA Glycosylase / Q000737:chemistry*
chemical_list: D004706:Endodeoxyribonucleases; C118712:MBD4 protein, human; D044502:Thymine DNA Glycosylase