title: Genome-wide association studies for osteochondrosis in French Trotter horses.
abstract: A genome-wide association study for osteochondrosis (OC) in French Trotter horses was carried out to detect QTL using genotype data from the Illumina EquineSNP50 BeadChip assay. Analysis data came from 161 sire families of French Trotter horses with 525 progeny and family sizes ranging from 1 to 20. Genotypes were available for progeny (n = 525) and sires with at least 2 progeny (n = 98). Radiographic data were obtained from progeny using at least 10 views to reveal OC. All radiographic findings were described by at least 2 veterinary experts in equine orthopedics, and severity indices (scores) were assigned based on the size and location of the lesion. Traits used were a global score, the sum of all severity scores lesions (GM, quantitative measurement), and the presence or absence of OC on the fetlock (FM), hock (HM), and other sites (other). Data were analyzed using 2 mixed models including fixed effects, polygenic effects, and SNP or haplotype cluster effects. By combining results with both methods at moderate evidence of association threshold P < 5 × 10(-5), this genome-wide association study displayed 1 region for GM on the Equus caballus chromosome (ECA) 13, 2 for HM on ECA 3 and 14, and 1 for other on ECA 15. One region on ECA 3 for HM represented the most significant hit (P = 3 × 10(-6)). By comparing QTL between traits at a decreased threshold (P < 5 × 10(-4)), the 4 QTL detected for GM were associated to a QTL detected for FM or HM but never both. Another interesting result was that no QTL were found in common between HM and FM.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR 631, 31326 Castanet-Tolosan, France. simon.teyssedre@toulouse.inra.fr
authors.forename: S
authors.initials: S
authors.lastname: Teyssèdre
authors.forename: M C
authors.initials: MC
authors.lastname: Dupuis
authors.forename: G
authors.initials: G
authors.lastname: Guérin
authors.forename: L
authors.initials: L
authors.lastname: Schibler
authors.forename: J M
authors.initials: JM
authors.lastname: Denoix
authors.forename: J M
authors.initials: JM
authors.lastname: Elsen
authors.forename: A
authors.initials: A
authors.lastname: Ricard
mesh_terms: D000818:Animals; D005260:Female; D005552:Forelimb / Q000000981:diagnostic imaging / Q000473:pathology; D005602:France; D055106:Genome-Wide Association Study; D005838:Genotype; D006239:Haplotypes; D006734:Horse Diseases / Q000000981:diagnostic imaging / Q000235:genetics* / Q000473:pathology; D006736:Horses; D007592:Joint Diseases / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D008297:Male; D008954:Models, Biological; D055034:Osteochondrosis / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D011859:Radiography; D013643:Tarsus, Animal / Q000000981:diagnostic imaging / Q000473:pathology