title: Comparative analysis of CRT Buffer, GC saliva check buffer tests and laboratory titration to evaluate saliva buffering capacity.
abstract: OBJECTIVE. The purpose of this study is to evaluate the ability of two commercial strip tests and laboratory titration to detect saliva buffer capacity. MATERIALS AND METHODS. Sixty-four patients were examined. Stimulated saliva was collected and buffer capacity was determined with two different chair-side strip tests in addition to immediate transportation to the laboratory to check the buffering ability by titrating with 0.005 M HCl and measuring pH by digital pH/Ion meter, used as a gold standart. The correlation were analyzed using the Spearman Rank Correlation Test, Cohen's Kappa coefficient and Pearson's Correlation test, p < 0.01. Sensitivity and specificity were used to measure precision of these tests. RESULTS. The response rate was 80%. High buffer capacity was found in 23.4% of cases, medium in 62.5%, and low in 14.1%. The Spearman Rank Correlation coefficient between the titration method and CRT Buffer test was 0.685 and the GC Saliva Check Buffer was 0.837. The Kappa coefficient for the CRT Buffer test was 0.508, while the coefficient for the GC Saliva Check Buffer was 0.752. The Pearson Correlation for the GC Saliva Check was 0.675. The difference is found in the buffer capacity at initial pH and at pH value 3. CONCLUSIONS. Both colorimetric tests correlate with the acid titration method in laboratory and are usable for saliva buffer capacity detection in dental offices. Buffer capacity detected in laboratory at different pH values can provide more information regarding caries risk.
authors.affiliation: Department of Therapeutic Dentistry, Institute of Stomatology, Riga Stradins University, Latvia. ilze.maldupa@rsu.lv
authors.forename: Ilze
authors.initials: I
authors.lastname: Maldupa
authors.forename: Anda
authors.initials: A
authors.lastname: Brinkmane
authors.forename: Anna
authors.initials: A
authors.lastname: Mihailova
mesh_terms: D000328:Adult; D002021:Buffers; D019411:Clinical Laboratory Techniques; D003124:Colorimetry; D003732:Dental Caries Activity Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D018570:Risk Assessment; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D011795:Surveys and Questionnaires; D055815:Young Adult
chemical_list: D002021:Buffers; D011933:Reagent Kits, Diagnostic