title: Validation of a vertical progression porcine burn model.
abstract: A major potential goal of burn therapy is to limit progression of partial- to full-thickness burns. To better test therapies, the authors developed and validated a vertical progression porcine burn model in which partial-thickness burns treated with an occlusive dressing convert to full-thickness burns that heal with scarring and wound contraction. Forty contact burns were created on the backs and flanks of two young swine using a 150 g aluminum bar preheated to 70°C, 80°C, or 90°C for 20 or 30 seconds. The necrotic epidermis was removed and the burns were covered with a polyurethane occlusive dressing. Burns were photographed at 1, 24, and 48 hours as well as at 7, 14, 21, and 28 days postinjury. Full-thickness biopsies were obtained at 1, 4, 24, and 48 hours as well as at 7 and 28 days. The primary outcomes were presence of deep contracted scars and wound area 28 days after injury. Secondary outcomes were depth of injury, reepithelialization, and depth of scars. Data were compared across burn conditions using analysis of variance and χ(2) tests. Eight replicate burns were created with the aluminum bar using the following temperature/contact-time combinations: 70/20, 70/30, 80/20, 80/30, and 90/20. The percentage of burns healing with contracted scars were 70/20, 0%; 70/30, 25%; 80/20, 50%; 80/30, 75%; and 90/20, 100% (P = .05). Wound areas at 28 days by injury conditions were 70/20, 8.1 cm(2); 70/30, 7.8 cm(2); 80/20, 6.6 cm(2); 80/30, 4.9 cm(2); and 90/20, 4.8 cm(2) (P = .007). Depth of injury judged by depth of endothelial damage for the 80/20 and 80/30 burns at 1 hour was 36% and 60% of the dermal thickness, respectively. The depth of injury to the endothelial cells 1 hour after injury was inversely correlated with the degree of scar area (Pearson's correlation r = -.71, P < .001). Exposure of porcine skin to an aluminum bar preheated to 80°C for 20 or 30 seconds results initially in a partial-thickness burn that when treated with an occlusive dressing progresses to a full-thickness injury and heals with significant scarring and wound contracture.
authors.affiliation: Department of Emergency Medicine, Stony Brook University, Stony Brook, New York, USA.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Singer
authors.forename: Douglas
authors.initials: D
authors.lastname: Hirth
authors.forename: Steve A
authors.initials: SA
authors.lastname: McClain
authors.forename: Laurie
authors.initials: L
authors.lastname: Crawford
authors.forename: Fubao
authors.initials: F
authors.lastname: Lin
authors.forename: Richard A F
authors.initials: RA
authors.lastname: Clark
mesh_terms: D000818:Animals; D001458:Bandages; D002056:Burns / Q000150:complications / Q000473:pathology* / Q000628:therapy*; D016009:Chi-Square Distribution; D002921:Cicatrix / Q000209:etiology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004727:Endothelium / Q000166:cytology; D005260:Female; D010784:Photomicrography; D013223:Statistics as Topic; D013552:Swine