title: Determination of the Pharmacokinetics and Oral Bioavailability of Salicylamine, a Potent γ-Ketoaldehyde Scavenger, by LC/MS/MS.
abstract: Levels of reactive γ-ketoaldehydes derived from arachidonate increase in diseases associated with inflammation and oxidative injury. To assess the biological importance of these γ-ketoaldehydes, we previously identified salicylamine as an effective γ-ketoaldehyde scavenger in vitro and in cells. To determine if salicylamine could be administered in vivo, we developed an LC/MS/MS assay to measure salicylamine in plasma and tissues. In mice, half-life (t(1/2)) was 62 minutes. Drinking water supplementation (1-10 g/L) generated tissue concentrations (10-500 μM) within the range previously shown to inhibit γ-ketoaldehydes in cells. Therefore, oral administration of salicylamine can be used to assess the contribution of γ-ketoaldehydes in animal models of disease.
authors.affiliation: Division of Clinical Pharmacology, Departments of Pharmacology, Medicine, and Pathology, Vanderbilt University, Nashville, TN, USA;  irene.zagol@vanderbilt.edu  (I.A.Z.-I.);  elena.matafonova@vanderbilt.edu  (E.M.);  venkataraman.amarnath@vanderbilt.edu  (V.A.);  christopher.l.bodine@vanderbilt.edu  (C.L.B.);  olivier.boutaud@vanderbilt.edu  (O.B.);  john.oates@vanderbilt.edu  (J.A.O.);  jack.roberts@vanderbilt.edu  (L.J.R.).
authors.forename: Irene A
authors.initials: IA
authors.lastname: Zagol-Ikapitte
authors.forename: Elena
authors.initials: E
authors.lastname: Matafonova
authors.forename: Venkataraman
authors.initials: V
authors.lastname: Amarnath
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Bodine
authors.forename: Olivier
authors.initials: O
authors.lastname: Boutaud
authors.forename: Rommel G
authors.initials: RG
authors.lastname: Tirona
authors.forename: John A
authors.initials: JA
authors.lastname: Oates
authors.forename: L Jackson
authors.initials: LJ
authors.lastname: Roberts
authors.forename: Sean S
authors.initials: SS
authors.lastname: Davies