title: B- and T-cell epitope mapping of human sapovirus capsid protein: an immunomics approach.
abstract: Human sapovirus is one of the major causes of viral gastroenteritis. Although the capsid protein (VP1) confers antigenic cross-reactivity, immunity against sapovirus is still unclear. Using immunoinformatics approach, we defined putative T- and B-cell epitopes of VP1 and mapped on to its predicted three-dimensional structure. Identified five putative T-cell epitopes also occupied the putative B-cell epitope region. These putative epitopes were conserved in all existing serotypes. Predicted epitopes can be generated through proteasome cleavage and may be useful in designing peptide-based subunit vaccine to confer both humoral and cell-mediated immunity.
authors.affiliation: Clinical Laboratory Services, International Centre for Diarrhoeal Disease Research, 68, Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka 1212, Bangladesh. ruhulmb@gmail.com
authors.forename: M Ruhul
authors.initials: MR
authors.lastname: Amin
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Siddiqui
authors.forename: Dilruba
authors.initials: D
authors.lastname: Ahmed
authors.forename: Firoz
authors.initials: F
authors.lastname: Ahmed
authors.forename: Anowar
authors.initials: A
authors.lastname: Hossain
mesh_terms: D000595:Amino Acid Sequence; D002213:Capsid; D036022:Capsid Proteins; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D006801:Humans; D008969:Molecular Sequence Data; D029341:Sapovirus
chemical_list: D036022:Capsid Proteins; D000939:Epitopes; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte