title: Preparation and characterization of catalase-loaded solid lipid nanoparticles protecting enzyme against proteolysis.
abstract: Catalase-loaded solid lipid nanoparticles (SLNs) were prepared by the double emulsion method (w/o/w) and solvent evaporation techniques, using acetone/methylene chloride (1:1) as an organic solvent, lecithin and triglyceride as oil phase and Poloxmer 188 as a surfactant. The optimized SLN was prepared by lecithin: triglyceride ratio (5%), 20-second + 30-second sonication, and 2% Poloxmer 188. The mean particle size of SLN was 296.0 ± 7.0 nm, polydispersity index range and zeta potential were 0.322-0.354 and -36.4 ± 0.6, respectively, and the encapsulation efficiency reached its maximum of 77.9 ± 1.56. Catalase distributed between the solid lipid and inner aqueous phase and gradually released from Poloxmer coated SLNs up to 20% within 20 h. Catalase-loaded SLN remained at 30% of H(2)O(2)-degrading activity after being incubated with Proteinase K for 24 h, while free catalase lost activity within 1 h.
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; E-Mails: jqzwuxi@163.com (Q.-Z.J.); wxg1002@hotmail.com (X.-G.W.).
authors.forename: Ce
authors.initials: C
authors.lastname: Qi
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Qing-Zhe
authors.initials: QZ
authors.lastname: Jing
authors.forename: Xing-Guo
authors.initials: XG
authors.lastname: Wang
mesh_terms: D000096:Acetone / Q000737:chemistry; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D004655:Emulsions / Q000737:chemistry; D019286:Endopeptidase K / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D008752:Methylene Chloride / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D059748:Proteolysis; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004655:Emulsions; D008055:Lipids; D013501:Surface-Active Agents; D000096:Acetone; D008752:Methylene Chloride; D006861:Hydrogen Peroxide; D002374:Catalase; D019286:Endopeptidase K