title: [Detection of extended-spectrum ß-lactamase-producing Enterobacteriaceae in rectal swabs with the Mastdiscs™ ID AmpC ßLSE detection set].
abstract: OBJECTIVE
  The double-disk synergy test was compared to the Mastdiscs™ ID AmpC and ESßL method for detection of ESßL production in rectal swab.
  
  
  METHODS
  Two hundred and forty-nine rectal swabs were directly inoculated onto Mueller-Hinton plates and analyzed according to both methods.
  
  
  RESULTS
  A total of 41 (16%) and 208 (84%) were positive and negative for ESßL, respectively. Twelve (29%) and 20 (49%) of the 41 rectal swabs positive for ESßL were detected after 24h of incubation with the double-disk synergy test and the Mastdiscs™ method, respectively (P=0.013). One hundred fifty-eight (76%) et 183 (88%) of the 208 rectal swabs were detected negative for ESßL after 24h of incubation with the double-disk synergy test and the Mastdiscs™ method, respectively (P<0.001). Finally, 79 (32%) and 46 (18%) rectal swabs respectively inoculated according to the double-disk synergy test and the Mastdiscs™ method were inconclusive after 24h of incubation. The better performance of the Mastdiscs™ method was due to an easier detection of cephalosporinase producing bacteria.
  
  
  CONCLUSIONS
  The Mastdiscs™ method is a simple phenotypic method that detects more easily ESßL and non-ESßL producing bacteria in rectal swab.
authors.affiliation: Laboratoire de bactériologie hygiène, centre de biologie pathologie, CHRU de Lille, boulevard Jules-Leclercq, 59000 Lille, France. nadine.lemaitre@chru-lille.fr
authors.forename: N
authors.initials: N
authors.lastname: Lemaître
authors.forename: C
authors.initials: C
authors.lastname: Loïez
authors.forename: N
authors.initials: N
authors.lastname: Pastourel
authors.forename: B
authors.initials: B
authors.lastname: Grandbastien
authors.forename: N
authors.initials: N
authors.lastname: Loukili
authors.forename: R
authors.initials: R
authors.lastname: Dessein
authors.forename: R
authors.initials: R
authors.lastname: Courcol
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004754:Enterobacter / Q000254:growth & development / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000302:isolation & purification* / Q000378:metabolism; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D007709:Klebsiella / Q000254:growth & development / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques / Q000295:instrumentation / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012007:Rectum / Q000382:microbiology*; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D011933:Reagent Kits, Diagnostic; C048795:AmpC beta-lactamases; D001618:beta-Lactamases