title: Adenomatous polyposis coli (APC) is essential for maintaining the integrity of the seminiferous epithelium.
abstract: Sertoli cells provide the microenvironment necessary for germ cell development and spermatogenesis; disruption of Sertoli cell morphology or function can lead to germ cell aplasia, which is observed in testicular dysgenesis syndrome. Mutation of the adenomatous polyposis coli (APC) gene has been associated with various human cancers, including testicular cancer, but its involvement in nonmalignant testicular pathologies has not been reported. We have developed a mouse model (APC(cko)) that expresses a truncated form of APC in Sertoli cells. Despite normal embryonic and early postnatal testicular development in APC(cko) mice, premature germ cell loss and Sertoli cell-only seminiferous tubules were observed in mutant testes without affecting Sertoli cell quiescence, apoptosis, or differentiation, which were confirmed by the absence of both proliferating cell nuclear antigen, DNA strand breaks, and anti-Müllerian hormone, respectively. We show that mutant Sertoli cells lose their apical extensions, which would normally enclose germ cells during various stages of spermatogenesis, and were unable to maintain the blood-testis barrier because of disrupted expression of junctional proteins. We also observed an up-regulation of Snail and Slug, markers suggestive of epithelial-mesenchymal transition in the Sertoli cells, but tumorigenesis was not observed. No comparable phenotype was observed with Sertoli cell-specific loss-of-function mutations in β-catenin, leading us to speculate that truncation of APC in Sertoli cells results in progressive degeneration of the seminiferous tubules by a mechanism that disrupts the integrity of Sertoli cell junctions independently of APC-regulated β-catenin activities and leads to development of a Sertoli cell-only phenotype.
authors.affiliation: Vincent Center for Reproductive Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA.
authors.forename: Pradeep S
authors.initials: PS
authors.lastname: Tanwar
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
authors.forename: Jose M
authors.initials: JM
authors.lastname: Teixeira
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000378:metabolism*; D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001814:Blood-Testis Barrier / Q000378:metabolism / Q000473:pathology; D015820:Cadherins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D022081:Cell Surface Extensions / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D008297:Male; D051379:Mice; D050505:Mutant Proteins / Q000378:metabolism; D021381:Protein Transport; D012670:Seminiferous Epithelium / Q000378:metabolism* / Q000473:pathology*; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology; D019108:Tight Junctions / Q000378:metabolism; D051176:beta Catenin / Q000172:deficiency / Q000378:metabolism
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D015415:Biomarkers; D015820:Cadherins; D050505:Mutant Proteins; D051176:beta Catenin