title: Complete sequence of Fig fleck-associated virus, a novel member of the family Tymoviridae.
abstract: The complete nucleotide sequence and the genome organization were determined of a novel virus, tentatively named Fig fleck-associated virus (FFkaV). The viral genome is a positive-sense, single-stranded RNA 7046 nucleotides in size excluding the 3'-terminal poly(A) tract, and comprising two open reading frames. ORF1 encodes a polypeptide of 2161 amino acids (p240), which contains the signatures of replication-associated proteins and the coat protein cistron (p24) at its 3' end. ORF2 codes for a 461 amino acid protein (p50) identified as a putative movement proteins (MP). In phylogenetic trees constructed with sequences of the putative polymerase and CP proteins FFkaV consistently groups with members of the genus Maculavirus, family Tymoviridae. However, the genome organization diverges from that of the two completely sequenced maculaviruses, Grapevine fleck virus (GFkV) and Bombix mori Macula-like virus (BmMLV), as it exhibits a structure resembling that of Maize rayado fino virus (MRFV), the type species of the genus Marafivirus and of Olive latent virus 3 (OLV-3), an unclassified virus in the family Tymoviridae. FFkaV was found in field-grown figs from six Mediterranean countries with an incidence ranging from 15% to 25%.
authors.affiliation: Istituto Agronomico Mediterraneo di Bari, Via Ceglie 9, 70010 Valenzano, Bari, Italy.
authors.forename: Toufic
authors.initials: T
authors.lastname: Elbeaino
authors.forename: Michele
authors.initials: M
authors.lastname: Digiaro
authors.forename: Giovanni P
authors.initials: GP
authors.lastname: Martelli
mesh_terms: D000595:Amino Acid Sequence; D030681:Ficus / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012526:Satellite Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D046830:Tymoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins