title: Identification of host genes involved in geminivirus infection using a reverse genetics approach.
abstract: Geminiviruses, like all viruses, rely on the host cell machinery to establish a successful infection, but the identity and function of these required host proteins remain largely unknown. Tomato yellow leaf curl Sardinia virus (TYLCSV), a monopartite geminivirus, is one of the causal agents of the devastating Tomato yellow leaf curl disease (TYLCD). The transgenic 2IRGFP N. benthamiana plants, used in combination with Virus Induced Gene Silencing (VIGS), entail an important potential as a tool in reverse genetics studies to identify host factors involved in TYLCSV infection. Using these transgenic plants, we have made an accurate description of the evolution of TYLCSV replication in the host in both space and time. Moreover, we have determined that TYLCSV and Tobacco rattle virus (TRV) do not dramatically influence each other when co-infected in N. benthamiana, what makes the use of TRV-induced gene silencing in combination with TYLCSV for reverse genetic studies feasible. Finally, we have tested the effect of silencing candidate host genes on TYLCSV infection, identifying eighteen genes potentially involved in this process, fifteen of which had never been implicated in geminiviral infections before. Seven of the analyzed genes have a potential anti-viral effect, whereas the expression of the other eleven is required for a full infection. Interestingly, almost half of the genes altering TYLCSV infection play a role in postranslational modifications. Therefore, our results provide new insights into the molecular mechanisms underlying geminivirus infections, and at the same time reveal the 2IRGFP/VIGS system as a powerful tool for functional reverse genetics studies.
authors.affiliation: Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, Málaga, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lozano-Durán
authors.forename: Tábata
authors.initials: T
authors.lastname: Rosas-Díaz
authors.forename: Ana P
authors.initials: AP
authors.lastname: Luna
authors.forename: Eduardo R
authors.initials: ER
authors.lastname: Bejarano
mesh_terms: D003001:Cloning, Molecular; D017791:Geminiviridae / Q000502:physiology*; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics; D056726:Genetic Association Studies; D049452:Green Fluorescent Proteins / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology*; D030821:Plants, Genetically Modified; D059386:Reverse Genetics / Q000379:methods*; D014026:Nicotiana / Q000235:genetics* / Q000821:virology*
chemical_list: D049452:Green Fluorescent Proteins