title: Oxidative stress and inflammation in renal patients and healthy subjects.
abstract: The first goal of this study was to measure the oxidative stress (OS) and relate it to lipoprotein variables in 35 renal patients before dialysis (CKD), 37 on hemodialysis (HD) and 63 healthy subjects. The method for OS was based on the ratio of cholesteryl esters (CE) containing C18/C16 fatty acids (R2) measured by gas chromatography (GC) which is a simple, direct, rapid and reliable procedure. The second goal was to investigate and identify a triacylglycerol peak on GC, referred to as TG48 (48 represents the sum of the three fatty acids carbon chain lengths) which was markedly increased in renal patients compared to healthy controls. We measured TG48 in patients and controls. Mass spectrometry (MS) and MS twice in tandem were used to analyze the fatty acid composition of TG48. MS showed that TG48 was abundant in saturated fatty acids (SFAs) that were known for their pro-inflammatory property. TG48 was significantly and inversely correlated with OS. Renal patients were characterized by higher OS and inflammation than healthy subjects. Inflammation correlated strongly with TG, VLDL-cholesterol, apolipoprotein (apo) C-III and apoC-III bound to apoB-containing lipoproteins, but not with either total cholesterol or LDL-cholesterol.In conclusion, we have discovered a new inflammatory factor, TG48. It is characterized with TG rich in saturated fatty acids. Renal patients have increased TG48 than healthy controls.
authors.affiliation: Lipid and Lipoprotein Studies, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States of America. alaupovicp@omrf.org
authors.forename: Diana M
authors.initials: DM
authors.lastname: Lee
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Jackson
authors.forename: Nicholas
authors.initials: N
authors.lastname: Knowlton
authors.forename: Joshua
authors.initials: J
authors.lastname: Wages
authors.forename: Petar
authors.initials: P
authors.lastname: Alaupovic
authors.forename: Ola
authors.initials: O
authors.lastname: Samuelsson
authors.forename: Aso
authors.initials: A
authors.lastname: Saeed
authors.forename: Michael
authors.initials: M
authors.lastname: Centola
authors.forename: Per-Ola
authors.initials: PO
authors.lastname: Attman
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002784:Cholesterol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000628:therapy; D008074:Lipoproteins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis / Q000009:adverse effects*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol