title: Hydroxylation modification and free radical scavenging activity of puerarin-7-O-fructoside.
abstract: Puerarin-7-O-fructoside was transformed by Trichoderma harzianum CGMCC 1523 into 3'-hydroxypuerarin-7-O-fructoside; this was identified by MS and NMR. However, puerarin-7-O-glucoside was not directly hydroxylated but hydrolyzed back into puerarin, which was transformed into 3'-hydroxypuerarin by the same fungi. Comparative analysis of free radical scavenging activity of DPPH showed that the free radical scavenging activity of puerarin-7-O-glucoside was reduced to approximately 1/2 of that of puerarin, while the free radical scavenging activity of puerarin-7-O-fructoside was increased to approximately 1.5 times of that of puerarin. The free radical scavenging activity of 3'-hydroxypuerarin-7-O-fructoside was further increased by 2.2 times of that of puerarin-7-O-fructoside, which was close to that of 3'-hydroxypuerarin.
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Science, Nanjing Normal University, 1 Wenyuan Rd, Nanjing, Jiangsu Province, China.
authors.forename: Guiyou
authors.initials: G
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Yin
authors.initials: Y
authors.lastname: Ji
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.forename: Guodong
authors.initials: G
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Yijun
authors.initials: Y
authors.lastname: Dai
authors.forename: Sheng
authors.initials: S
authors.lastname: Yuan
mesh_terms: D001711:Biotransformation; D016166:Free Radical Scavengers / Q000737:chemistry / Q000378:metabolism*; D005632:Fructose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006900:Hydroxylation; D007529:Isoflavones / Q000737:chemistry / Q000378:metabolism*; D014242:Trichoderma / Q000378:metabolism
chemical_list: D016166:Free Radical Scavengers; D007529:Isoflavones; C568770:puerarin-7-O-fructoside; D005632:Fructose