title: Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus.
abstract: We performed a series of experiments with Geobacillus stearothermophilus, a thermophile isolated from oil-contaminated soil in the Kuwaiti desert. The organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. In the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (CDCA). Five transformed products, namely, cholic acid, methylcholate, methylchenodeoxycholate, 3α-hydroxy-7-oxo-5β-cholanic acid, and 7α-hydroxy-3-oxo-5β-cholanic acid, were the major transformation products catalyzed by G. stearothermophilus. Under aerobic conditions, no evidence of side chain degradation, ring cleavage, or dehydrogenation was found among the metabolites of CDCA. CDCA transformation by a thermophile is reported for the first time.
authors.affiliation: Department of Biological Sciences, Kuwait University, Kuwait City. afzalq8@gmail.com
authors.forename: Mohammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Sosamma
authors.initials: S
authors.lastname: Oommen
authors.forename: Samira
authors.initials: S
authors.lastname: Al-Awadi
mesh_terms: D001711:Biotransformation; D002635:Chenodeoxycholic Acid / Q000737:chemistry / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000302:isolation & purification / Q000378:metabolism*; D006358:Hot Temperature; D007730:Kuwait; D012988:Soil Microbiology
chemical_list: D002635:Chenodeoxycholic Acid