title: Characterization of short-term temperature, exposure, and solubilization effects on library compound quality.
abstract: Many compound collections are stored under the same temperature conditions, which can limit flexibility by increasing the processing time required for high-demand compounds. In this study, the authors wanted to evaluate the impact of a hybrid-storage approach where high-demand compounds are stored for a shortened time period at room temperature to expedite processing operations. The use of a Covaris adaptive-focused acoustics platform was also characterized as a potential enhancement or alternative to storage at elevated temperatures. This study evaluated the impact of temperature, exposure, and solubilization on overall compound quality for short-term storage. A small library of 25 representative compounds was evaluated over an 18-week period to monitor the change in purity and concentration by high-performance liquid chromatography with ultraviolet detection. The authors concluded that temperature had a significant impact on compound concentration, and the effects due to exposure cycles were minimal. A storage time of 12 weeks at room temperature resulted in minimal compound loss, but storage times beyond this would be unacceptable because of a >20% decrease in concentration. Finally, the acoustic solubilization protocol also increased the number of compounds at the target concentration with no impact on overall purity, leading to a potential for increased storage times at frozen temperatures.
authors.affiliation: Discovery Analytical Sciences, Chemistry Research and Discovery, Amgen, Inc., Thousand Oaks, CA 91320, USA. jeschelb@amgen.com
authors.forename: John W
authors.initials: JW
authors.lastname: Eschelbach
authors.forename: Dorothy
authors.initials: D
authors.lastname: Zhuomei
authors.forename: Breanne
authors.initials: B
authors.lastname: Grady
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Goetzinger
mesh_terms: D004355:Drug Stability; D004356:Drug Storage; D013058:Mass Spectrometry; D012996:Solutions; D012997:Solvents; D013696:Temperature; D013997:Time Factors
chemical_list: D012996:Solutions; D012997:Solvents