title: The signal peptide sequence impacts the immune response elicited by a DNA epitope vaccine.
abstract: We examined the effect of two leader sequences, one from a transmembrane molecule (H2-L(d)) and another from a secreted molecule (rat KC chemokine), on the immunogenicity of DNA epitope vaccines. The chemokine leader enhanced vaccine immunogenicity, thus underscoring the importance of the leader sequence in DNA epitope vaccine design.
authors.affiliation: Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA. dvatakis@ucla.edu
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Vatakis
authors.forename: Minnie
authors.initials: M
authors.lastname: McMillan
mesh_terms: D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology*; D007117:Immunization, Secondary / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D021382:Protein Sorting Signals / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000939:Epitopes; D007376:Interleukin-2; D021382:Protein Sorting Signals; D019444:Vaccines, DNA; D007371:Interferon-gamma