title: Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining.
abstract: After the third prezygotic division during conjugation of Paramecium caudatum, migratory and stationary pronuclei are produced. The migratory pronuclei remain in the paroral region tightly against the conjugating boundaries; while the stationary pronuclei are located beside the migratory pronuclei. To date, however, it is not clear what causes this close side-by-side localization between migratory and stationary pronuclei. In the current study, immunofluorescence staining with monoclonal antibody of anti-α tubulin indicated that ''U'' or ''V'' shaped spindles connected the migratory and stationary pronuclei during the third prezygotic division. This observation accounts for the close localization between these two types of pronuclei.
authors.affiliation: The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin'an 311300, China. wildlife909@yahoo.cn
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.forename: Jia-Jun
authors.initials: JJ
authors.lastname: Zhu
authors.forename: Xian-Yu
authors.initials: XY
authors.lastname: Yang
authors.forename: Jin-Qiang
authors.initials: JQ
authors.lastname: Yuan
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.forename: Min-Guo
authors.initials: MG
authors.lastname: Song
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics*; D003227:Conjugation, Genetic; D005455:Fluorescent Antibody Technique; D008540:Meiosis; D048488:Paramecium caudatum / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D013194:Staining and Labeling