title: The core planar cell polarity gene prickle interacts with flamingo to promote sensory axon advance in the Drosophila embryo.
abstract: The atypical cadherin Drosophila protein Flamingo and its vertebrate homologues play widespread roles in the regulation of both dendrite and axon growth. However, little is understood about the molecular mechanisms that underpin these functions. Whereas flamingo interacts with a well-defined group of genes in regulating planar cell polarity, previous studies have uncovered little evidence that the other core planar cell polarity genes are involved in regulation of neurite growth. We present data in this study showing that the planar cell polarity gene prickle interacts with flamingo in regulating sensory axon advance at a key choice point - the transition between the peripheral nervous system and the central nervous system. The cytoplasmic tail of the Flamingo protein is not required for this interaction. Overexpression of another core planar cell polarity gene dishevelled produces a similar phenotype to prickle mutants, suggesting that this gene may also play a role in regulation of sensory axon advance.
authors.affiliation: Department of Anatomy and Cell Biology, University of Melbourne, VIC 3010, Australia. e.mrkusich@gmail.com
authors.forename: Eli M
authors.initials: EM
authors.lastname: Mrkusich
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Flanagan
authors.forename: Paul M
authors.initials: PM
authors.lastname: Whitington
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000502:physiology*; D015820:Cadherins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D000072261:Dishevelled Proteins; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D060588:LIM Domain Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D034622:RNA Interference; D011984:Sensory Receptor Cells / Q000166:cytology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; D004268:DNA-Binding Proteins; D000072261:Dishevelled Proteins; D029721:Drosophila Proteins; D060588:LIM Domain Proteins; D010750:Phosphoproteins; C000606588:dsh protein, Drosophila; C121600:pk protein, Drosophila; C121420:stan protein, Drosophila