title: Association of L55M and Q192R polymorphisms of paraoxonase-1 gene with preeclampsia.
abstract: OBJECTIVE
  Although preeclampsia is one of the most common complications of pregnancy, the exact etiology of preeclampsia is unknown. Paraoxonase-1 (PON1) is a high-density lipoprotein-associated enzyme, which exhibits antioxidative and antiatherogenic activities. This study was aimed to determine the possible association between L55M and Q192R polymorphisms of paraoxonase-1 gene as well as serum PON1 and arylesterase (ARE) activities in preeclampsia.
  
  
  METHODS
  This case-control study was performed on 105 preeclampsia patients and 84 normal healthy pregnant women. The polymorphisms of PON1 were determined using tetra ARMS-PCR method and serum paroxonase (PON1) and arylesterase (ARE) activities were measured spectrophotometrically.
  
  
  RESULTS
  Results showed that 192 QR genotype of PON1 was higher among preeclampsia patients (43.8%) than control subjects (31.0%) (crude OR = 1.92, 95% CI, 1.03-3.57; adjusted OR = 2.07, 95% CI, 1.04-4.16,). The R allele is a risk factor for preeclampsia (OR = 1.64, 95% CI, 1.03-2.60, p = 0.038). There were no significant differences regarding L55M genotypes or alleles frequencies of PON1 among groups (p >0.05). We found that serum paraoxonase and aryesterase activities were significantly higher in preeclampsia than controls (p <0.05).
  
  
  CONCLUSIONS
  PON1 allele 192R is a risk factor for preeclampsia. The clinical significance of an increase of serum PON1 and ARE activities in preeclampsia remains to be elucidated. More studies in larger samples with different ethnic groups are required to validate our findings.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaei
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.forename: Azin
authors.initials: A
authors.lastname: Azarian
authors.forename: Abdolkarim
authors.initials: A
authors.lastname: Moazeni-Roodi
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mokhtari
authors.forename: Anoosh
authors.initials: A
authors.lastname: Naghavai
authors.forename: Saeideh
authors.initials: S
authors.lastname: Salimi
authors.forename: Mahdi
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Mohsen
authors.initials: M
authors.lastname: Taheri
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghavami
mesh_terms: D043303:Aryldialkylphosphatase / Q000097:blood / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D012307:Risk Factors
chemical_list: D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D043303:Aryldialkylphosphatase