title: A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor.
abstract: The TRAPP vesicle-tethering complex consists of more than 10 distinct polypeptides and is involved in protein transport. Using the C2 subunit as bait we identified SPATA4, a spermatocyte-specific protein of unknown function, as an interacting partner in a yeast two hybrid screen. Further studies indicate SPATA4 interacts with the C2 portion of the TRAPP complex. SPATA4 fractionates with both cytosolic and nuclear fractions suggesting it may have several distinct functions. SPATA4 is one of only three human proteins that contain a DUF1042 domain and we show that C2 does not interact with another one of the DUF1042 domain-containing proteins. Our results suggest a role for SPATA4 in membrane traffic and a specialized function for TRAPP in spermatocytes.
authors.affiliation: Concordia University, Department of Biology, Montreal, Quebec, Canada.
authors.forename: Débora Teixeira
authors.initials: DT
authors.lastname: Duarte
authors.forename: Sokunthear
authors.initials: S
authors.lastname: Hul
authors.forename: Michael
authors.initials: M
authors.lastname: Sacher
mesh_terms: D006801:Humans; D047468:Immunoprecipitation; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D026901:Membrane Transport Proteins; D011506:Proteins; D011994:Recombinant Proteins; C457219:SPATA4 protein, human; C120759:TRAPPC2 protein, human; D014157:Transcription Factors