title: Performance of two different Limulus amebocyte lysate assays for the quantitation of fungal glucan.
abstract: This study examined the response of various forms and sources of glucans toward two different Limulus amebocyte lysate (LAL) methods, the modified LAL, and Glucatell. The glucans studied were curdlan, laminarin, yeast glucan, barley glucan, paramylon, pullulan, pustulan, mannan, and pachyman (as part of the Glucatell kit). Both methods provided largely similar results for each of the glucans; however, the Glucatell method yielded slightly higher responses to certain structures that may not necessarily be of fungal origin, leading to falsely greater positive results. The performance of each method to measure fungal glucan concentration specifically was then assessed.
authors.affiliation: Department of Chemistry, Carleton University, Ottawa, Ontario, Canada.
authors.forename: Hafsa
authors.initials: H
authors.lastname: Cherid
authors.forename: Mark
authors.initials: M
authors.lastname: Foto
authors.forename: J David
authors.initials: JD
authors.lastname: Miller
mesh_terms: D004784:Environmental Monitoring / Q000379:methods*; D005658:Fungi / Q000737:chemistry*; D005936:Glucans / Q000032:analysis*; D008033:Limulus Test; D013045:Species Specificity
chemical_list: D005936:Glucans