title: Proteome reference map and regulation network of neonatal rat cardiomyocyte.
abstract: OBJECTIVE
  To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte.
  
  
  METHODS
  Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server.
  
  
  RESULTS
  More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn.
  
  
  CONCLUSIONS
  A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data.
authors.affiliation: Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Ministry of Health and Key Laboratory of Molecular Cardiology, Ministry of Education, Beijing 100191, China.
authors.forename: Zi-jian
authors.initials: ZJ
authors.lastname: Li
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Qi-de
authors.initials: QD
authors.lastname: Han
authors.forename: You-yi
authors.initials: YY
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008297:Male; D053858:Metabolic Networks and Pathways; D032383:Myocytes, Cardiac / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D020543:Proteome