title: Fusibacter tunisiensis sp. nov., isolated from an anaerobic reactor used to treat olive-mill wastewater.
abstract: Strain BELH1(T), a novel mesophilic, anaerobic, halotolerant, rod-shaped bacterium, was isolated from a Tunisian wastewater digester. The cells of the strain are motile, measure 0.5×2-5 µm, and occur singly or in pairs. The strain reduced thiosulfate and elemental sulfur (but not sulfate or sulfite) into sulfide. It grew at 15-40 °C (optimum 30 °C), pH 5.8-8.4 (optimum 7) and with 0-10 % (w/v) NaCl (optimum 3.0 %). The genomic DNA G+C content of strain BELH1(T) was 38.2 mol% and the strain's predominant cellular fatty acids were C(14:0), a summed feature that contained iso-C(17:1) and/or anteiso-C(17:1) B, and C(16:0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel strain was most closely related to Fusibacter paucivorans (94.8 % sequence similarity). Based on phenotypic, phylogenetic and taxonomic characteristics, strain BELH1(T) represents a novel species of the genus Fusibacter, for which the name Fusibacter tunisiensis sp. nov. is proposed. The type strain is BELH1(T) ( = DSM 24436(T) = JCM 17481(T)).
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis, Tunisia.|Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Wajdi
authors.initials: W
authors.lastname: Ben Hania
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis, Tunisia.|Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Belkis
authors.initials: B
authors.lastname: Fraj
authors.affiliation: Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Postec
authors.affiliation: Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Khaled
authors.initials: K
authors.lastname: Fadhlaoui
authors.affiliation: Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis, Tunisia.
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.affiliation: Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Ollivier
authors.affiliation: Laboratoire de Microbiologie M10, UMR 2359, Aix-Marseille Univ., Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000693:Anaerobiosis; D001482:Base Composition; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D031658:Olea / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000032:analysis / Q000382:microbiology*; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013885:Thiosulfates