title: Cellular assay optimization: part II: the use of a simple integrated robotic work cell to allow the multiplexed batching of cellular assays.
abstract: This report describes the implementation of an automated work cell with commercially available hardware and software, capable of handling up to 15 separate reagents for performing 96-well or 384-well assays but with a small footprint and only a single liquid dispenser and two plate washers. Extremely flexible software was used to enable this simple work cell to perform processes that would traditionally require a much larger, more expensive automation platform. With the development of the C-Myc assays for the targets DYRK, BMX, PERK, and FAK, the authors describe a software solution to multibatch assays to run simultaneously, reducing reagent dead volume and increasing the efficiency of running multiple assays such that the time to generate data across multiple targets was significantly shortened. Although a larger automated system with multiple robotic arms and extensive equipment would also be able to process multiple assays simultaneously, the work cell we have described represents an inexpensive and flexible, easily upgradable option suitable for a wider range of labs.
authors.affiliation: Marie A. Macmillan was contracted to AstraZeneca via Morson International, Adamson House, Centenary Way, Salford, Manchester, UK.
authors.forename: Marie A
authors.initials: MA
authors.lastname: Macmillan
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Orme
authors.forename: Karen
authors.initials: K
authors.lastname: Roberts
mesh_terms: D001681:Biological Assay / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000592:standards*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012371:Robotics; D012680:Sensitivity and Specificity
chemical_list: D016271:Proto-Oncogene Proteins c-myc