title: Modulation of inflammatory processes by leaves extract from Clusia nemorosa both in vitro and in vivo animal models.
abstract: The present study was carried out to investigate the anti-inflammatory effect of the hexane extract of the leaves from Clusia nemorosa G. Mey, called HECn, using carrageenan-induced mice pleurisy and cotton pellet-induced mice granuloma. Additionally, the ability of HECn to affect both neutrophil migration as viability was investigated by use of the Boyden chamber assay and flow cytometry, respectively. The HECn significantly inhibited exudation, total leukocytes and neutrophils influx, as well as TNFα levels in carrageenan-induced pleurisy. However, the extract not suppressed the granulomatous tissue formation in the cotton pellet-induced granuloma test. Experiments performed in vitro revealed that HECn on human neutrophils inhibited a dose-dependent manner the CXCL1-induced neutrophil chemotaxis. Furthermore, HECn also inhibited the chemoattraction of human neutrophils induced by formyl-methionyl-leucyl-phenylalanine (fMLP), leukotriene B4 (LTB4) and platelet activating factor (PAF) in a Boyden chamber. However, this same treatment not was able to induce apoptosis. The results obtained in this study showed that the extract from leaves of C. nemorosa possess a potent inhibitory activity in acute model of inflammation, being the effects mediated, in part, by inhibition of neutrophil responsiveness. These results indicate that C. nemorosa could be a good source for anti-inflammatory compounds.
authors.affiliation: Laboratório de Biologia Celular, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, Maceió, Alagoas, Brazil.
authors.forename: José Alex C
authors.initials: JA
authors.lastname: Farias
authors.forename: Jamylle Nunes S
authors.initials: JN
authors.lastname: Ferro
authors.forename: Juliane P
authors.initials: JP
authors.lastname: Silva
authors.forename: Isabela Karine R
authors.initials: IK
authors.lastname: Agra
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Oliveira
authors.forename: André Luiz P
authors.initials: AL
authors.lastname: Candea
authors.forename: Fernando P
authors.initials: FP
authors.lastname: Conte
authors.forename: Fausto K
authors.initials: FK
authors.lastname: Ferraris
authors.forename: Maria das Graças M O
authors.initials: Md
authors.lastname: Henriques
authors.forename: Lucia M
authors.initials: LM
authors.lastname: Conserva
authors.forename: Emiliano
authors.initials: E
authors.lastname: Barreto
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002351:Carrageenan; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D031251:Clusia; D032561:Cotton Fiber; D006099:Granuloma / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D051379:Mice; D009504:Neutrophils / Q000276:immunology / Q000502:physiology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan