title: [Preparation and application of a colloidal gold strip to detect the rabies antibody].
abstract: To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect the rabies antibody in clinical sample from immuned dogs by rabies vaccine. Colloidal gold particles labeled with purified rabies virus (CVS11) were used as the detector reagent. The staphylococcal protein A (SPA) and pured rabbit anti-rabies virus IgG were blotted on the test and control regions of nitrocellulose membrane. Then the strip was assembled with sample pad, absorbing pad, and dorsal shield. The assay samples (261 dog's serum) were collected from Wildlife Rabies Disease Diagnostic Laboratories of Ministry of Agriculture in China, Institute of Military Veterinary, Academy of Military Medical Sciences and other six provinces, including rabies virus positive and negative serum. The performance of the strip was compared to fluorescent antibody virus neutralization test. The neutralizing antibody titer could be detected above 0.5 IU. The strip did not change of performance when stored at room temperature for 12 months. It may offer reference of neutralizing antibody titer level after dogs immuned rabies vaccine and determin whether the dogs need to be immuned again.
authors.affiliation: Key Laboratory of Jilin Provice for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130122, China.
authors.forename: Tiecheng
authors.initials: T
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Songtao
authors.initials: S
authors.lastname: Yang
authors.forename: Hualei
authors.initials: H
authors.lastname: Wang
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Jin
authors.forename: Pingsen
authors.initials: P
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Feng
authors.forename: Geng
authors.initials: G
authors.lastname: Huang
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Zou
authors.forename: Xianzhu
authors.initials: X
authors.lastname: Xia
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000032:analysis / Q000097:blood; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood*; D002846:Chromatography, Affinity / Q000379:methods; D004285:Dogs; D017979:Gold Colloid; D011818:Rabies / Q000517:prevention & control / Q000662:veterinary*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D014611:Vaccination
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D017979:Gold Colloid; D011819:Rabies Vaccines; D011934:Reagent Strips