title: Activity of ACHN-490 against meticillin-resistant Staphylococcus aureus (MRSA) isolates from patients in US hospitals.
abstract: The activity of ACHN-490 was evaluated against 493 meticillin-resistant Staphylococcus aureus (MRSA) isolates collected in 2009-2010 from 23 US hospitals. The MIC(50) and MIC(90) values (minimal inhibitory concentrations for 50% and 90% of the organisms, respectively) for ACHN-490 were 1 and 2 μg/mL compared with 8 and 32 μg/mL for amikacin, 0.5 and 1 μg/mL for gentamicin and 2 and >16 μg/mL for tobramycin. The gene encoding the aminoglycoside-modifying enzyme APH(2″)-Ia/AAC(6')-Ie was present in 12% of the subset of 84 isolates examined by polymerase chain reaction (PCR), whilst the gene encoding ANT(4')-Ia was present in 89% of isolates. ACHN-490 activity was not affected by either enzyme.
authors.affiliation: Cepheid, 904 Caribbean Drive, Sunnyvale, CA 94089, USA. fred.tenover@cepheid.com
authors.forename: Fred C
authors.initials: FC
authors.lastname: Tenover
authors.forename: Isabella
authors.initials: I
authors.lastname: Tickler
authors.forename: Eliana S
authors.initials: ES
authors.lastname: Armstrong
authors.forename: Aya
authors.initials: A
authors.lastname: Kubo
authors.forename: Sara
authors.initials: S
authors.lastname: Lopez
authors.forename: David H
authors.initials: DH
authors.lastname: Persing
authors.forename: George H
authors.initials: GH
authors.lastname: Miller
mesh_terms: D000617:Aminoglycosides / Q000235:genetics / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001769:Blood / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006761:Hospitals; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D009666:Nose / Q000382:microbiology; D012853:Sisomicin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D014031:Tobramycin / Q000494:pharmacology; D014481:United States
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C550938:plazomicin; D014031:Tobramycin; D012853:Sisomicin