title: Structural insight into the stereoselective production of PGF2α by Old Yellow Enzyme from Trypanosoma cruzi.
abstract: Old yellow enzyme (OYE) is an NADPH oxidoreductase capable of reducing a variety of compounds. It contains flavin mononucleotide (FMN) as a prosthetic group. A ternary complex structure of OYE from Trypanosoma cruzi (TcOYE) with FMN and one of the substrates, p-hydroxybenzaldehyde, shows a striking movement around the active site upon binding of the substrate. From a structural comparison of other OYE complexed with 12-oxophytodienoate, we have constructed a complex structure with another substrate, prostaglandin H(2) (PGH(2)), to provide a proposed stereoselective reaction mechanism for the reduction of PGH(2) to prostaglandin F(2α) by TcOYE.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamada-Oka, Suita, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Keishi
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Keiji
authors.initials: K
authors.lastname: Tokuoka
authors.forename: Nahoko
authors.initials: N
authors.lastname: Uchiyama
authors.forename: Shigeru
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Koji
authors.initials: K
authors.lastname: Inaka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D001547:Benzaldehydes / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D015237:Dinoprost / Q000737:chemistry / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000737:chemistry / Q000378:metabolism; D009252:NADPH Dehydrogenase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D001547:Benzaldehydes; D015800:Protozoan Proteins; D005486:Flavin Mononucleotide; D015237:Dinoprost; D009252:NADPH Dehydrogenase; C011483:4-hydroxybenzaldehyde