title: Phenolic chemical composition of Petroselinum crispum extract and its effect on haemostasis.
abstract: From the aqueous extract (Pc) of Petroselinum crispum (Mill) flat leaves specimens were isolated and identified the flavonoids apigenin (1), apigenin-7-O-glucoside or cosmosiin (2), apigenin-7-O-apiosyl-(1 --> 2)-O-glucoside or apiin (3) and the coumarin 2",3"-dihydroxyfuranocoumarin or oxypeucedanin hydrate (4). The inhibitory activity toward clotting formation and platelet aggregation was assessed for Pc flavonoids (1) and (2), and the coumarin (4). Pc showed no inhibition on clotting activity when compared with the control. On the other hand, a strong antiplatelet aggregation activity was observed for Pc (IC50 = 1.81 mg/mL), apigenin (IC50 = 0.036 mg/mL) and cosmosiin (IC50 = 0.18 mg/mL). In all cases ADP was used as inductor of platelet aggregation. Our results showed that Pc, apigenin and cosmosiin interfere on haemostasis inhibiting platelet aggregation. To the best of our knowledge this is the first report for the cosmosiin antiplatelet aggregation in vitro activity.
authors.affiliation: Nucleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21 941-902, Rio de Janeiro, RJ, Brazil.
authors.forename: Douglas S A
authors.initials: DS
authors.lastname: Chaves
authors.forename: Flávia S
authors.initials: FS
authors.lastname: Frattani
authors.forename: Mariane
authors.initials: M
authors.lastname: Assafim
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: de Almeida
authors.forename: Russolina B
authors.initials: RB
authors.lastname: de Zingali
authors.forename: Sônia S
authors.initials: SS
authors.lastname: Costa
mesh_terms: D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009904:Optical Rotation; D010314:Partial Thromboplastin Time; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011517:Prothrombin Time; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors