title: [Study of galic acid extracted from the leaves of Phyllanthus emblica on apoptotic mechanism of human hepatocellular carcinoma cells BEL-7404].
abstract: OBJECTIVE
  To explore the mechanism of apoptosis of human hepatocellular carcinoma cells BEL-7404 induced by Galic acid extracted from leaves of Phyllanthus emblica.
  
  
  METHODS
  PI/Hoechst33342 double staining method was utilized to observe the influence on cell life cycle. The expressions of Bax and Bcl-2 protein were determined by fluorescence immunostaining and Western blot.
  
  
  RESULTS
  The results from PI/Hoechst33342 double staining method indicated that the percentage of cells G2/M phases increased after treated for 72 h, the phenomenon of blockage appeared and cell death was induced. Fluorescence immunostaining showed that the expression of Bax was up-regulated, and the expression of Bcl-2 was down-regulated. Western blot also showed that the the expression of Bax was up-regulated.
  
  
  CONCLUSIONS
  The mechanism of human hepatoma BEL-7404 cells apoptosis induced by Galic acid from leaves of Phyllanthus emblica may be blocking G2/M period in cell life cycle, up-regulating the expression of Bax and down-regulating the expression of Bcl-2, that can decrease membrane potential of mitochondria,and triggered the caspases of activation of cascade and induced cell death.
authors.affiliation: Center of Research & Development of New Drugs, Guangxi Traditional Chinese Medical University, Nanning 530001, China. hjl0221104@163.com
authors.forename: Jin-lan
authors.initials: JL
authors.lastname: Huang
authors.forename: Zhen-guo
authors.initials: ZG
authors.lastname: Zhong
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005707:Gallic Acid / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D029842:Phyllanthus emblica / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D005707:Gallic Acid