title: Measurement of peroxiredoxin activity.
abstract: Peroxiredoxins are cysteine-dependent peroxidases that react with hydrogen peroxide, larger hydroperoxide substrates, and peroxynitrite. Protocols are provided to measure Prx activity with peroxide by (1) a coupled reaction with NADPH, thioredoxin reductase, and thioredoxin, (2) the direct monitoring of thioredoxin oxidation, (3) competition with horseradish peroxidase, and (4) peroxide consumption using the FOX assay.
authors.affiliation: Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA.
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Nelson
authors.forename: Derek
authors.initials: D
authors.lastname: Parsonage
mesh_terms: D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D006801:Humans; D054464:Peroxiredoxins / Q000378:metabolism* / Q000502:physiology; D013379:Substrate Specificity
chemical_list: D054464:Peroxiredoxins