title: Evaluation of pooling strategies for acute HIV-1 infection screening using nucleic acid amplification testing.
abstract: A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a specimen with 1,827 copies/ml was nonreactive in one of three replicates.
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY 12201-2002, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Sullivan
authors.forename: Pragna
authors.initials: P
authors.lastname: Patel
authors.forename: Angela
authors.initials: A
authors.lastname: Hutchinson
authors.forename: Steven F
authors.initials: SF
authors.lastname: Ethridge
authors.forename: Monica M
authors.initials: MM
authors.lastname: Parker
mesh_terms: D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008403:Mass Screening / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010949:Plasma / Q000821:virology*; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D013048:Specimen Handling / Q000379:methods*; D014773:Virology / Q000379:methods*
chemical_list: D012367:RNA, Viral