title: Microfluidic baker's transformation device for three-dimensional rapid mixing.
abstract: We developed a new passive-type micromixer based on the baker's transformation and realized a fast mixing of a protein solution, which has lower diffusion constant. The baker's transformation is an ideal mixing method, but there is no report on the microfluidic baker's transformation (MBT), since it is required to fabricate the complicated three-dimensional (3D) structure to realize the MBT device. In this note, we successfully fabricate the MBT device by using precision diamond cutting of an oxygen-free copper substrate for the mould fabrication and PDMS replication. The MBT device with 10.4 mm mixing length enables us to achieve complete mixing of a FITC solution (D = 2.6 × 10(-10) m(2) s(-1)) within 51 ms and an IgG solution (D = 4.6 × 10(-11) m(2) s(-1)) within 306 ms. Its mixing speed is 70-fold higher for a FITC solution and 900-fold higher for an IgG solution than the mixing speed by the microchannel without MBT structures. The Péclet number to attain complete mixing in the MBT device is estimated to be 6.9 × 10(4).
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. yasui.takao@e.mbox.nagoya-u.ac.jp
authors.forename: Takao
authors.initials: T
authors.lastname: Yasui
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Omoto
authors.forename: Keiko
authors.initials: K
authors.lastname: Osato
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.forename: Norikazu
authors.initials: N
authors.lastname: Suzuki
authors.forename: Toyohiro
authors.initials: T
authors.lastname: Naito
authors.forename: Masaki
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.forename: Eiji
authors.initials: E
authors.lastname: Shamoto
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012996:Solutions / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D007074:Immunoglobulin G; D012996:Solutions; C013830:baysilon; D016650:Fluorescein-5-isothiocyanate