title: Cytoplasmic Ig alpha serine/threonines fine-tune Ig alpha tyrosine phosphorylation and limit bone marrow plasma cell formation.
abstract: Igα serine 191 and 197 and threonine 203, which are located in proximity of the Igα ITAM, dampen Igα ITAM tyrosine phosphorylation. In this study, we show that mice with targeted mutations of Igα S191, 197, and T203 displayed elevated serum IgG2c and IgG2b concentrations and had elevated numbers of IgG2c- and IgG2b-secreting cells in the bone marrow. BCR-induced Igα tyrosine phosphorylation was slightly increased in splenic B cells. Our results suggest that Igα serine/threonines limit formation of IgG2c- and IgG2b-secreting bone marrow plasma cells, possibly by fine-tuning Igα tyrosine-mediated BCR signaling.
authors.affiliation: Program in Cellular and Molecular Medicine at Children's Hospital Boston and Immune Disease Institute, Boston, MA 02115, USA.
authors.forename: Heide Christine
authors.initials: HC
authors.lastname: Patterson
authors.forename: Manfred
authors.initials: M
authors.lastname: Kraus
authors.forename: Donghai
authors.initials: D
authors.lastname: Wang
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Shahsafaei
authors.forename: Joel M
authors.initials: JM
authors.lastname: Henderson
authors.forename: Jane
authors.initials: J
authors.lastname: Seagal
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Otipoby
authors.forename: To-Ha
authors.initials: TH
authors.lastname: Thai
authors.forename: Klaus
authors.initials: K
authors.lastname: Rajewsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D003593:Cytoplasm / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015151:Immunoblotting; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics / Q000276:immunology*; D010766:Phosphorylation; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D012694:Serine / Q000737:chemistry / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013912:Threonine / Q000737:chemistry / Q000276:immunology; D014443:Tyrosine / Q000378:metabolism
chemical_list: D011947:Receptors, Antigen, B-Cell; D013912:Threonine; D014443:Tyrosine; D012694:Serine