title: ENZO: a web tool for derivation and evaluation of kinetic models of enzyme catalyzed reactions.
abstract: We describe a web tool ENZO (Enzyme Kinetics), a graphical interface for building kinetic models of enzyme catalyzed reactions. ENZO automatically generates the corresponding differential equations from a stipulated enzyme reaction scheme. These differential equations are processed by a numerical solver and a regression algorithm which fits the coefficients of differential equations to experimentally observed time course curves. ENZO allows rapid evaluation of rival reaction schemes and can be used for routine tests in enzyme kinetics. It is freely available as a web tool, at http://enzo.cmm.ki.si.
authors.affiliation: National Institute of Chemistry, Ljubljana, Slovenia.
authors.forename: Staš
authors.initials: S
authors.lastname: Bevc
authors.forename: Janez
authors.initials: J
authors.lastname: Konc
authors.forename: Jure
authors.initials: J
authors.lastname: Stojan
authors.forename: Milan
authors.initials: M
authors.lastname: Hodošček
authors.forename: Matej
authors.initials: M
authors.lastname: Penca
authors.forename: Matej
authors.initials: M
authors.lastname: Praprotnik
authors.forename: Dušanka
authors.initials: D
authors.lastname: Janežič
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D055162:Biocatalysis / Q000187:drug effects; D002092:Butyrylthiocholine / Q000494:pharmacology; D020134:Catalytic Domain; D002401:Cathepsin B / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000378:metabolism*; D020407:Internet; D007700:Kinetics; D008954:Models, Biological; D012015:Reference Standards; D013379:Substrate Specificity / Q000187:drug effects; D017186:Titrimetry; D014101:Torpedo / Q000378:metabolism
chemical_list: D004798:Enzymes; D002092:Butyrylthiocholine; D000110:Acetylcholinesterase; D002401:Cathepsin B