title: Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.
abstract: Extremely low-frequency magnetic fields (ELF-MFs) may affect human health because of the possible associations with leukemia but also with cancer, cardiovascular, and neurological disorders. In the present work, human SH-SY5Y neuroblastoma cells were exposed to a 50 Hz, 1 mT sinusoidal ELF-MF at three different times, that is, 5 days (T5), 10 days (T10), and 15 days (T15) and then the effects of ELF-MF on proteome expression and biological behavior were investigated. Through comparative analysis between treated and control samples, we analyzed the proteome changes induced by ELF-MF exposure. Nine new proteins resolved in sample after a 15-day treatment were involved in a cellular defense mechanism and/or in cellular organization and proliferation such as peroxiredoxin isoenzymes (2, 3, and 6), 3-mercaptopyruvate sulfurtransferase, actin cytoplasmatic 2, t-complex protein subunit beta, ropporin-1A, and profilin-2 and spindlin-1. Our results indicated that ELF-MFs exposure altered the proliferative status and other important cell biology-related parameters, such as cell growth pattern, and cytoskeletal organization. These findings support our hypothesis that ELF radiation could trigger a shift toward a more invasive phenotype.
authors.affiliation: Department of Biomedical Science, G. d'Annunzio University, Chieti-Pescara, Italy.
authors.forename: Marilisa
authors.initials: M
authors.lastname: Sulpizio
authors.forename: Stefano
authors.initials: S
authors.lastname: Falone
authors.forename: Fernanda
authors.initials: F
authors.lastname: Amicarelli
authors.forename: Marco
authors.initials: M
authors.lastname: Marchisio
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Di Giuseppe
authors.forename: Enrica
authors.initials: E
authors.lastname: Eleuterio
authors.forename: Carmine
authors.initials: C
authors.lastname: Di Ilio
authors.forename: Stefania
authors.initials: S
authors.lastname: Angelucci
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D007150:Immunohistochemistry; D008280:Magnetics; D009447:Neuroblastoma / Q000473:pathology*; D020543:Proteome
chemical_list: D020543:Proteome