title: Stoichiometry of reconstituted high-density lipoproteins in the hydrated state determined by photon antibunching.
abstract: Apolipoprotein A-I plays a central role in the solution structure of high-density lipoproteins. Determining the stoichiometry of lipid-bound apo A-I in the hydrated state is therefore fundamental to understanding how high-density lipoproteins form and function. Here, we use the quantum optical phenomenon of photon antibunching to determine the number of apo A-I molecules bound to discoidal lipoproteins and compare this with values obtained by photon-counting histogram analysis. Both the photon antibunching and photon-counting analyses show that reconstituted high-density lipoprotein particles contain two apo A-I molecules, which is in agreement with the commonly accepted double-belt model.
authors.affiliation: Physical and Life Science Directorate, Lawrence Livermore National Laboratory, Livermore, California, USA.
authors.forename: Sonny
authors.initials: S
authors.lastname: Ly
authors.forename: Jitka
authors.initials: J
authors.lastname: Petrlova
authors.forename: Thomas
authors.initials: T
authors.lastname: Huser
authors.forename: Samantha
authors.initials: S
authors.lastname: Fore
authors.forename: Tingjuan
authors.initials: T
authors.lastname: Gao
authors.forename: John
authors.initials: J
authors.lastname: Voss
authors.forename: Ted A
authors.initials: TA
authors.lastname: Laurence
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry / Q000378:metabolism; D001703:Biophysics / Q000379:methods*; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000378:metabolism; D017785:Photons; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D014867:Water