title: Effect of ultrasound and dexpanthenol on collagen organization in tegumentary lesions.
abstract: OBJECTIVE
  To analyze the effect of ultrasound (US), dexpanthenol (d-P) and a combination of these treatments (US+d-P) on collagen fiber organization in tegumentary lesions in rats by birefringence analysis.
  
  
  METHODS
  Wistar rats (50) were anesthetized (Thionembutal - Sodic = 50 mg/Kg), 1 cm² of dorsal region skin was removed, and the animals were divided into five groups: control (C), gel (G), US (3 MHz, 0.1 W/cm2, 1 minute, continuous), d-P (10%) and US+d-P. After daily treatment for 7 and 14 days, 6 µm thick sections of lesioned areas were stained in picrosirius and measurements of the collagen birefringent area (µm²) were obtained using polarized light microscopy (Zeiss Axiolab-ZEISS- Germany) with histological image analysis software (KS 400 2.0 - Kontrol Eletronics, Munique, Germany). The means were compared by ANOVA followed by the Tukey test (p<0.05).
  
  
  RESULTS
  The US+d-P group showed a significantly greater (p ≤ 0.001) birefringent area (1586.43±162.14) than the other experimental groups: C (139.36±35.35), US (317.55±129.9) and d-P (192.41±3657) by the 7th day of treatment, indicating acceleration of the wound healing process. By the 14th day of treatment, the US+d-P, US and d-P groups presented greater birefringence than the control group, but did not differ from each other.
  
  
  CONCLUSIONS
  The combination of treatments (US+d-P) accelerated collagen fiber synthesis and organization in the early stages of cutaneous repair.
authors.affiliation: Faculdade de Ciências da Saúde, Universidade Metodista de Piracicaba, Piracicaba (UNIMEP), SP, Brasil.
authors.forename: Gustavo N
authors.initials: GN
authors.lastname: Guimarães
authors.forename: Maria S M
authors.initials: MS
authors.lastname: Pires-De-Campos
authors.forename: Gislaine R
authors.initials: GR
authors.lastname: Leonardi
authors.forename: Helena H K
authors.initials: HH
authors.lastname: Dib-Giusti
authors.forename: Maria L O
authors.initials: ML
authors.lastname: Polacow
mesh_terms: D000818:Animals; D003094:Collagen / Q000187:drug effects*; D003131:Combined Modality Therapy; D005260:Female; D010205:Pantothenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014464:Ultrasonic Therapy; D014945:Wound Healing
chemical_list: D010205:Pantothenic Acid; C007288:dexpanthenol; D003094:Collagen