title: Discrimination of neutral oligosaccharides through a nanopore.
abstract: The detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single α-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1→4 and 1→6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device.
authors.affiliation: CNRS UMR 8587, Laboratoire Analyse et Environnement, Université d'Évry Val d'Essonne, Évry, France. laurent.bacri@univ-evry.fr
authors.forename: Laurent
authors.initials: L
authors.lastname: Bacri
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Oukhaled
authors.forename: Erven
authors.initials: E
authors.lastname: Hémon
authors.forename: Fenseth Banzouzi
authors.initials: FB
authors.lastname: Bassafoula
authors.forename: Loïc
authors.initials: L
authors.lastname: Auvray
authors.forename: Régis
authors.initials: R
authors.lastname: Daniel
mesh_terms: D001427:Bacterial Toxins / Q000737:chemistry*; D003911:Dextrans / Q000032:analysis; D006460:Hemolysin Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008051:Lipid Bilayers / Q000737:chemistry*; D008320:Maltose / Q000032:analysis; D058608:Nanopores; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D001427:Bacterial Toxins; D003911:Dextrans; D006460:Hemolysin Proteins; D008051:Lipid Bilayers; D009844:Oligosaccharides; C021091:staphylococcal alpha-toxin; D008320:Maltose