title: [The study of plasma proteomic changes in a patient with high-altitude cerebral edema].
abstract: OBJECTIVE
  High-altitude cerebral edema (HACE) is one of the most serious acute mountain sickness and its underlying mechanism is still unknown clearly. The aim of this study was to determine the changes of plasma proteins in high altitude cerebral edema patients for discovering essential biomarkers used for the routine prophylaxis, diagnosis and treatment.
  
  
  METHODS
  Plasma protein profiling two dimensional gel electrophoresis followed by mass spectrometry was used to explore protein alterations in one patient with high-altitude cerebral edema (HACE). Striking differences in two-dimensional gel proteomes of plasma were observed between high-altitude cerebral edema (HACE) and high-altitude pulmonary edema (HAPE) and between high-altitude cerebral edema (HACE) and mild acute mountain sickness (mAMS). Furthermore, apolipoprotein E altered in high-altitude cerebral edema was validated by ELISA.
  
  
  RESULTS
  Different six spots were found in this study from the comparison between HACE and HAPE, and there were different six spots which were detected from the plasma of HACE patient in comparison to mAMS. Apolipoprotein E was identified in the two groups of comparative maps and results of ELISA consisted with the results of 2-DE.
  
  
  CONCLUSIONS
  In this study, we used proteomic approach to explore HACE firstly and found different proteins that were probably associated with HACE. This would offer a clue to a better understanding of HACE for precaution, diagnosis and treatment.
authors.affiliation: Cardiovascular Drug Research Center, Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Rui-Feng
authors.initials: RF
authors.lastname: Duan
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000328:Adult; D000531:Altitude; D000532:Altitude Sickness / Q000150:complications*; D001057:Apolipoproteins E / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D001929:Brain Edema / Q000097:blood* / Q000209:etiology; D005260:Female; D006801:Humans; D040901:Proteomics / Q000379:methods*
chemical_list: D001057:Apolipoproteins E; D001798:Blood Proteins