title: Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects.
abstract: Mir-290 through mir-295 (mir-290-295) is a mammalian-specific microRNA (miRNA) cluster that, in mice, is expressed specifically in early embryos and embryonic germ cells. Here, we show that mir-290-295 plays important roles in embryonic development as indicated by the partially penetrant lethality of mutant embryos. In addition, we show that in surviving mir-290-295-deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290-295(-/-) mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290-295(-/-) mice are unable to recover and are sterile, due to premature ovarian failure.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA.
authors.forename: Lea A
authors.initials: LA
authors.lastname: Medeiros
authors.forename: Lucas M
authors.initials: LM
authors.lastname: Dennis
authors.forename: Mark E
authors.initials: ME
authors.lastname: Gill
authors.forename: Hristo
authors.initials: H
authors.lastname: Houbaviy
authors.forename: Styliani
authors.initials: S
authors.lastname: Markoulaki
authors.forename: Dongdong
authors.initials: D
authors.lastname: Fu
authors.forename: Amy C
authors.initials: AC
authors.lastname: White
authors.forename: Oktay
authors.initials: O
authors.lastname: Kirak
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Sharp
authors.forename: David C
authors.initials: DC
authors.lastname: Page
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis; D002452:Cell Count; D002453:Cell Cycle; D020964:Embryo Loss / Q000235:genetics* / Q000473:pathology*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology; D005260:Female; D005298:Fertility / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000378:metabolism* / Q000473:pathology*; D006066:Gonads / Q000254:growth & development / Q000473:pathology; D007247:Infertility, Female / Q000235:genetics / Q000473:pathology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019683:Penetrance
chemical_list: C547384:MIRN290 microRNA, mouse; C561933:MIRN295 microRNA, mouse; D035683:MicroRNAs