title: Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: importance in normal cell growth, stress resistance, and longevity.
abstract: Heat shock transcription factor (HSF), a key regulator in the expression of heat shock protein (HSP) chaperones, is involved in the maintenance of protein homeostasis. However, the impact of HSF-mediated transcription of each HSP gene on this process is not fully understood. We show that Saccharomyces cerevisiae cells containing mutations in the HSF-binding sequences of chromosomal HSP90 promoters exhibit various phenotypes, including slow growth, proteotoxic stress sensitivity, and reduced chronological lifespan. Similar phenotypes were observed when HSF-binding sequences in five mitochondrial HSP promoters were mutated. Therefore, HSF-regulated changes in expression of these chaperone genes are necessary to maintain cell viability under various growth conditions.
authors.affiliation: Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa, Ishikawa, Japan. sakurai@kenroku.kanazawa-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakurai
authors.forename: Azumi
authors.initials: A
authors.lastname: Ota
mesh_terms: D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D002470:Cell Survival / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D003958:Diamide / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D024483:Vitamin K 3 / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; C067168:HSF1 protein, S cerevisiae; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D003958:Diamide; D000431:Ethanol; D001383:Azetidinecarboxylic Acid; D024483:Vitamin K 3; D011494:Protein Kinases; C410736:Rim15 protein, S cerevisiae