title: Proteolipidic composition of exosomes changes during reticulocyte maturation.
abstract: During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large amounts of hemoglobin, while eliminating numerous cellular components. Exosomes are small secreted vesicles that play an important role in this process of specific elimination. To understand the mechanisms of proteolipidic sorting leading to their biogenesis, we have explored changes in the composition of exosomes released by reticulocytes during their differentiation, in parallel to their physical properties. By combining proteomic and lipidomic approaches, we found dramatic alterations in the composition of the exosomes retrieved over the course of a 7-day in vitro differentiation protocol. Our data support a previously proposed model, whereby in reticulocytes the biogenesis of exosomes involves several distinct mechanisms for the preferential recruitment of particular proteins and lipids and suggest that the respective prominence of those pathways changes over the course of the differentiation process.
authors.affiliation: CNRS, Institute of Pharmacology and Structural Biology, 205 Route de Narbonne, France. kevin.carayon@ipbs.fr
authors.forename: Kévin
authors.initials: K
authors.lastname: Carayon
authors.forename: Karima
authors.initials: K
authors.lastname: Chaoui
authors.forename: Elsa
authors.initials: E
authors.lastname: Ronzier
authors.forename: Ikrame
authors.initials: I
authors.lastname: Lazar
authors.forename: Justine
authors.initials: J
authors.lastname: Bertrand-Michel
authors.forename: Véronique
authors.initials: V
authors.lastname: Roques
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Balor
authors.forename: François
authors.initials: F
authors.lastname: Terce
authors.forename: André
authors.initials: A
authors.lastname: Lopez
authors.forename: Laurence
authors.initials: L
authors.lastname: Salomé
authors.forename: Etienne
authors.initials: E
authors.lastname: Joly
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism*; D006454:Hemoglobins / Q000096:biosynthesis; D008297:Male; D008563:Membrane Lipids / Q000096:biosynthesis*; D008565:Membrane Proteins / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D008563:Membrane Lipids; D008565:Membrane Proteins