title: The importance of inter-species cell-cell co-aggregation between Lactobacillus plantarum ML11-11 and Saccharomyces cerevisiae BY4741 in mixed-species biofilm formation.
abstract: Cells of Lactobacillus plantarum ML11-11, an isolate from Fukuyama pot vinegar, and the yeast Saccharomyces cerevisiae formed significant mixed-species biofilms with concurrent inter-species co-aggregation. The co-aggregation did not occur with heated or proteinase K-treated ML11-11 cells, or in the presence of D-mannose, suggesting that surface proteins of ML11-11 and mannose-containing surface substance(s) of yeast were the predominant contributing factors. Sugar fatty acid ester inhibited mixed-species biofilm formation, but did not inhibit co-aggregation, suggesting that the cell-cell adhesion and cell-polystylene adhesion are controlled by different mechanisms. Microscopic observation and microflora analysis revealed that inter-species co-aggregation plays an important role in the formation of the mixed-species biofilm.
authors.affiliation: Department of Food Bioscience and Biotechnology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Natsumi
authors.initials: N
authors.lastname: Nojima
authors.forename: Kanako
authors.initials: K
authors.lastname: Yoshida
authors.forename: Satoru
authors.initials: S
authors.lastname: Hirayama
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morinaga
mesh_terms: D001422:Bacterial Adhesion; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002448:Cell Adhesion; D004952:Esters / Q000494:pharmacology; D005227:Fatty Acids / Q000494:pharmacology; D005656:Fungal Proteins / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000502:physiology*; D008358:Mannose / Q000494:pharmacology; D059013:Microbial Consortia / Q000187:drug effects / Q000502:physiology*; D056265:Microbial Interactions / Q000187:drug effects / Q000502:physiology*; D008853:Microscopy; D011137:Polystyrenes / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004952:Esters; D005227:Fatty Acids; D005656:Fungal Proteins; D011137:Polystyrenes; D008358:Mannose