title: Polymer based biosensor for rapid electrochemical detection of virus infection of human cells.
abstract: The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy. Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.
authors.affiliation: Technical University of Denmark, Department of Micro- and Nanotechnology, Oersteds Plads 345 East, DK-2800 Kongens Lyngby, Denmark.
authors.forename: Katrine
authors.initials: K
authors.lastname: Kiilerich-Pedersen
authors.forename: Claus R
authors.initials: CR
authors.lastname: Poulsen
authors.forename: Titoo
authors.initials: T
authors.lastname: Jain
authors.forename: Noemi
authors.initials: N
authors.lastname: Rozlosnik
mesh_terms: D017209:Apoptosis; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000302:isolation & purification*; D058266:Dielectric Spectroscopy / Q000379:methods*; D006801:Humans; D008839:Microelectrodes; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry*
chemical_list: D011108:Polymers