title: Applicability of an on-site test for its use in post-mortem blood.
abstract: The number of deaths related to drugs of abuse makes necessary the use of an on-site test for those cases in which a rapid detection of the consumed drug is required. Cozart® DDS test provides a simple, fast and reliable tool for the qualitative on-site analysis in post-mortem blood. Owing that this test is prepared for oral fluid samples, a validation becomes essential in order to use it for a different matrix than the established one. According to that, results obtained by Cozart® DDS test used in post-mortem blood samples have been compared with a qualitative gas chromatography-mass spectrometry (GC/MS/MS). Positive results for cocaine family compounds (COC-F) were 43.75%, for opiates family compounds (OPI-F) 25.78%, and for cannabis family compounds (THC-F) 2.34%. Negative results were 28.13%. No amphetamines (AMP) or methamphetamines (MA) were found. Sensitivity and specificity was available for cocaine and opiates but not for cannabis because only five cases were detected. Sensitivity, specificity, predictive positive value and predictive negative value for cocaine were 98%, 91%, 88% and 99%, respectively. Sensivilty, specificity, predictive positive value (PPV) and predictive negative value (NPV) for opiates were 93%, 92%, 76% and 98%, respectively. Likelihood positive ratios for cocaine and opiates have been 10.92 and 11.69, respectively, while likelihood negative ratios have been 0.02 and 0.08, respectively. Results show the suitability of Cozart® DDS test for the qualitative detection of cocaine and opiates in post-mortem blood.
authors.affiliation: Institut de Medicina Legal de Catalunya, Laboratory Department, Barcelona, Spain. amparo.arroyo@xij.gencat.net
authors.forename: Amparo
authors.initials: A
authors.lastname: Arroyo
authors.forename: Marta
authors.initials: M
authors.lastname: Sánchez
authors.forename: Mariona
authors.initials: M
authors.lastname: Palahí
authors.forename: Maria
authors.initials: M
authors.lastname: Barbal
authors.forename: M A Teresa
authors.initials: MA
authors.lastname: Marrón
authors.forename: Agustí
authors.initials: A
authors.lastname: Mora
mesh_terms: D000701:Analgesics, Opioid / Q000097:blood*; D003042:Cocaine / Q000097:blood*; D049429:Forensic Pathology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007118:Immunoassay; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000295:instrumentation*
chemical_list: D000701:Analgesics, Opioid; D011933:Reagent Kits, Diagnostic; D003042:Cocaine