title: Multifunctional manganese carbonate microspheres with superparamagnetic and fluorescent properties: synthesis and biological application.
abstract: Multifunctional manganese carbonate microspheres with superparamagnetic and fluorescent properties were fabricated and used as biological labels. The Fe(3)O(4)@MnCO(3) microspheres were synthesized by direct co-precipitation without any linker shell. The Fe(3)O(4)@MnCO(3) microspheres have uniform size distribution and rough surface, which provides a promising template for the assembly of polyelectrolytes (PEs) and CdTe quantum dots (QDs). A luminescent CdTe shell was observed in Fe(3)O(4)@MnCO(3)@PE-CdTe spheres by confocal fluorescence imaging. With excellent solubility in water and rough surfaces, the multifunctional microsphere offers a friendly microenvironment for immobilization of α-fetoprotein (AFP) antibodies (Ab(2)) to fabricate Fe(3)O(4)@MnCO(3)@PE-CdTe-Ab(2) architecture. By using the Fe(3)O(4) @MnCO(3)@PEs-CdTe-Ab(2) bioconjugate as a label, a promising and versatile platform for fluorescence imaging and electrochemical immunosensing of cancer biomarker AFP was developed. The prepared electrochemical immunosensor shows high sensitivity and selectivity with a detection limit of 0.3 pg mL(-1).
authors.affiliation: State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Peng
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Feng
authors.forename: Kui
authors.initials: K
authors.lastname: Zhang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Li
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Jiang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D002254:Carbonates / Q000138:chemical synthesis* / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D060328:Magnetic Phenomena; D008345:Manganese / Q000737:chemistry; D008863:Microspheres; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D002254:Carbonates; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D000509:alpha-Fetoproteins; C000499:ferric oxide; D008345:Manganese; C045327:manganese carbonate