title: C-linked antifreeze glycoprotein (C-AFGP) analogues as novel cryoprotectants.
abstract: Significant cell damage occurs during cryopreservation resulting in a decreased number of viable and functional cells post-thawing. Recent studies have correlated the unsuccessful outcome of regenerative therapies with poor cell viability after cryopreservation. Cell damage from ice recrystallization during freeze-thawing is one cause of decreased viability after cryopreservation. We have assessed the ability of two C-AFGPs that are potent inhibitors of ice recrystallization to increase cell viability after cryopreservation. Our results indicate that a 1-1.5 mg/mL (0.5-0.8 mM) solution of C-AFGP 1 is an excellent alternative to a 2.5% DMSO solution for the cryopreservation of human embryonic liver cells.
authors.affiliation: Department of Chemistry, Regenerative Medicine Program, University of Ottawa, Ontario, Canada.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Leclère
authors.forename: Bonnie K
authors.initials: BK
authors.lastname: Kwok
authors.forename: Luke K
authors.initials: LK
authors.lastname: Wu
authors.forename: David S
authors.initials: DS
authors.lastname: Allan
authors.forename: Robert N
authors.initials: RN
authors.lastname: Ben
mesh_terms: D021301:Antifreeze Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007053:Ice; D008099:Liver / Q000166:cytology / Q000196:embryology
chemical_list: D021301:Antifreeze Proteins; D003451:Cryoprotective Agents; D007053:Ice