title: Cytotoxic and antioxidant activities of selected Lamiales species from Mexico.
abstract: BACKGROUND
  Methanol extracts obtained from eight species belonging to four families of the Lamiales order native to Mexico were investigated for biological action.
  
  
  OBJECTIVE
  Cytotoxicity and antioxidant activity of methanol extracts have been investigated.
  
  
  METHODS
  Cytotoxic activity was evaluated by the sulphorhodamine B protein staining assay against KB (nasopharyngeal), HEp-2 (larynx), HF-6 (colon), MCF7 (breast), PC-3 (prostate), and Ca Ski (cervix) carcinoma cell lines. To analyze the antioxidant activity, common stable radicals chromogens, 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS+) and DPPH (1,1-diphenyl-2-picrylhydrazyl) were used. The ferric reducing antioxidant power (FRAP) and the total phenolic content of the samples were also determined.
  
  
  RESULTS
  Some of the extracts, such as Limosella aquatica L. (Scrophulariaceae), Mimulus glabratus Kunth. (Phrymaceae), Pedicularis mexicana Zucc. ex Benth. (Orobanchaceae), and Penstemon campanulatus (Cav.) Willd. (Plantaginaceae) displayed remarkably selective cytotoxic activity. However, the extract from Veronica americana (Raf.) Schwein (Plantaginaceae) showed the highest activity with IC₅₀ values of 1.46 and 0.169 g/ μL on PC-3 and HF-6 cells, respectively. With the exception of M. glabratus, all the extracts showed different degrees of antioxidant activity with IC₅₀ values from 0.89 up to 1.8 in the ABTS assay; from 0.49 up to 1.25 mg/mL in the DPPH assay and with the FRAP evaluation, 36 to 68 equivalents in mM of FeSO₄. V. americana also showed the highest antoxidant activity with IC₅₀ values from 0.491 and 0.892 mg/mL, on DPPH and ABTS assays, respectively.
  
  
  CONCLUSIONS
  These findings demonstrated that the species studied have great potential cytotoxic and antioxidant activity.
authors.affiliation: Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Morelos, México.
authors.forename: J A
authors.initials: JA
authors.lastname: Moreno-Escobar
authors.forename: S
authors.initials: S
authors.lastname: Bazald A
authors.forename: M L
authors.initials: ML
authors.lastname: Villarreal
authors.forename: J R
authors.initials: JR
authors.lastname: Bonilla-Barbosa
authors.forename: S
authors.initials: S
authors.lastname: Mendoza
authors.forename: V
authors.initials: V
authors.lastname: Rodríguez-López
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds; D045744:Cell Line, Tumor; D002839:Chromans / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D005290:Ferric Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D008800:Mexico; D010084:Oxidation-Reduction; D010636:Phenols / Q000737:chemistry; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D001713:Biphenyl Compounds; D002839:Chromans; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; C004931:1,1-diphenyl-2-picrylhydrazyl; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid