title: Optical encoding of microbeads based on silica particle encapsulated quantum dots and its applications.
abstract: A novel method concerning the coding technology of polystyrene beads with Si encapsulated quantum dot (QD) particles (Si@QDs particles) is studied in this paper. In the reverse microemulsion system containing tetraethoxysilane (TEOS), water-soluble QDs (emission peak at 600 nm) were enveloped within the silica shell, forming Si@QDs particles. The Si@QDs particles were characterized by TEM, showing good uniform size, with an average diameter of about 167.0 nm. In comparison with the pure water-soluble QDs, the encapsulation of water-soluble QDs in the silica shell led to an enhancement in anti-photobleaching by providing inert barriers for the QDs. Images presented by SEM and confocal laser scanning microscopy demonstrated that the Si@QDs particles were equably coated on the surface of carboxyl functionalized polystyrene (PS) beads. Then, with the assistance of ethyl-3-(dimethyl aminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS), human IgG could be successfully crosslinked to Si@QDs particle coated PS-COOH beads. Furthermore, the Si@QDs coated PS-COOH beads with human IgG were examined in immunoassay experiments, and the results indicated that these beads could be applied in the specific recognition of goat-anti-human IgG in solution. This investigation is expected to provide a new route to bead coding in the field of suspension microarrays, based on the use of QDs.
authors.affiliation: Key Laboratory of Biomedical Photonics of Ministry of Education-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, HuBei 430074, People's Republic of China. Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, HuBei, 430074, People's Republic of China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Zhu
authors.forename: Yuan-Cheng
authors.initials: YC
authors.lastname: Cao
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Hua
authors.forename: Hai-Qiao
authors.initials: HQ
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian-Hao
authors.initials: JH
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.forename: Yuan-Di
authors.initials: YD
authors.lastname: Zhao