title: [Study on molecular recognition technology in active constituents extracted and isolated from Aconitum pendulum].
abstract: OBJECTIVE
  To investigate CD molecular recognition technology applied in active constituents extracted and isolated from traditional Chinese medicine--Aconitum pendulum.
  
  
  METHODS
  The inclusion constant and form probability of the inclusion complex of Aconitum pendulum with p-CD was calculated by UV spectra method. The active constituents of Aconitum pendulum were extracted and isolated by molecular recognition technology. The inclusion complex was identified by UV. The chemical constituents of Aconitum pendulum and inclusion complex was determined by HPLC. The analgesic effects of inclusion complex was investigated by experiment of intraperitoneal injection of acetic acid in rats.
  
  
  RESULTS
  The inclusion complex was identified and confirmed by UV spectra method, the chemical components of inclusion complex were simple, and the content of active constituents increased significantly, the analgesic effects of inclusion complex was well.
  
  
  CONCLUSIONS
  The molecular recognition technology can be used for extracting and isolating active constituents of Aconitum pendulum, and the effects are obvious.
authors.affiliation: Department of Pharmacy, Ningxia Medical University, Yinchuan 750004, China. maxueqin217@126.com
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Ma
authors.forename: Guo-Shan
authors.initials: GS
authors.lastname: Li
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Fu
authors.forename: Jing-Zu
authors.initials: JZ
authors.lastname: Ma
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000737:chemistry; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D005260:Female; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D015394:Molecular Structure; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000700:Analgesics; D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D012997:Solvents; D047392:beta-Cyclodextrins; D000431:Ethanol