title: Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen.
abstract: This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.
authors.affiliation: Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, 14053 Caen, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Miszczak
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Shuck
authors.forename: Zhengchun
authors.initials: Z
authors.lastname: Lu
authors.forename: Yun Young
authors.initials: YY
authors.lastname: Go
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Zhang
authors.forename: Stephen
authors.initials: S
authors.lastname: Sells
authors.forename: Astrid
authors.initials: A
authors.lastname: Vabret
authors.forename: Stéphane
authors.initials: S
authors.lastname: Pronost
authors.forename: Guillaume
authors.initials: G
authors.lastname: Fortier
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Timoney
authors.forename: Udeni B R
authors.initials: UB
authors.lastname: Balasuriya
mesh_terms: D000818:Animals; D018174:Arterivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D007240:Equartevirus / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology; D006736:Horses; D018189:Immunomagnetic Separation / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012661:Semen / Q000821:virology*; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods; D014773:Virology / Q000379:methods
chemical_list: D012367:RNA, Viral