title: Poly(ethylene oxide) layers grafted to dopamine-melanin anchoring layer: stability and resistance to protein adsorption.
abstract: In this study, we propose substrate-independent modification for creating a protein-repellent surface based on dopamine-melanin anchoring layer used for subsequent binding of poly(ethylene oxide) (PEO) from melt. We verified that the dopamine-melanin layer can be formed on literally any substrate and could serve as the anchoring layer for subsequent grafting of PEO chains. Grafting of PEO from melt in a temperature range 70-110 °C produces densely packed PEO layers showing exceptionally low protein adsorption when exposed to the whole blood serum or plasma. The PEO layers prepared from melt at 110 °C retained the protein repellent properties for as long as 10 days after their exposure to physiological-like conditions. The PEO-dopamine-melanin modification represents a simple and universal surface modification method for the preparation of protein repellent surfaces that could serve as a nonfouling background in various applications, such as optical biosensors and tissue engineering.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky sq. 2, 162 06 Prague 6, Czech Republic. georgievski@imc.cas.cz
authors.forename: Ognen
authors.initials: O
authors.lastname: Pop-Georgievski
authors.forename: Štĕpán
authors.initials: Š
authors.lastname: Popelka
authors.forename: Milan
authors.initials: M
authors.lastname: Houska
authors.forename: Dagmar
authors.initials: D
authors.lastname: Chvostová
authors.forename: Vladimír
authors.initials: V
authors.lastname: Proks
authors.forename: František
authors.initials: F
authors.lastname: Rypáček
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000138:chemical synthesis*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008543:Melanins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001798:Blood Proteins; D020099:Coated Materials, Biocompatible; D008543:Melanins; C081789:dopamine melanin; D011092:Polyethylene Glycols