title: Identification and expression analysis of a novel transcript of the human PRMT2 gene resulted from alternative polyadenylation in breast cancer.
abstract: The arginine N-methyltransferase 2 protein (PRMT2, also known as HRMT1L1) is thought to act as a coactivator of ERα. The present results show the occurrence of a novel transcript by alternative polyadenylation in the human PRMT2 gene. We demonstrated that the newly identified intron-retaining PRMT2L2 transcript is functionally intact, efficiently translated into protein in vivo. PRMT2 and PRMT2L2 mRNA expression profiles overlap with the distribution of ERα, with the strongest abundance in estrogen target tissues. Transient co-transfection assays demonstrated that PRMT2L2 enhance ERα-mediated transactivation activity of ERE-Luc in a ligand-dependent manner. Confocal microscopy scanning revealed a distinct intra-cellular localization of their fusion proteins, suggesting that the C-terminal region absent in PRMT2L2 is critical for the localization. Statistical analysis further showed that both PRMT2 and PRMT2L2 mRNAexpressions were up-regulated in breast cancer tissues, and significantly associated with ERα positivity status. Thus, post-transcriptional processing mechanism as alternative polyadenylation and splicing may play a crucial role in regulating human PRMT2 gene expression.
authors.affiliation: University of South China, Hengyang 421001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.forename: Ren-Xian
authors.initials: RX
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Hong
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xu-Yu
authors.initials: XY
authors.lastname: Zu
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Xiao
authors.forename: Jiang-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Ge-Bo
authors.initials: GB
authors.lastname: Wen
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D026723:Polyadenylation / Q000235:genetics*; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D018076:DNA, Complementary; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; D049452:Green Fluorescent Proteins; C077013:PRMT2 protein, human; D011484:Protein-Arginine N-Methyltransferases