title: Fabrication and characterization of gold-TiO2 core-shell nanowire for immobilization of alkaline protease.
abstract: In this work, we report enhancement of the stability and biocatalytic activities of the alkaline protease enzyme immobilized on a gold/TiO2 core-shell nanowires. Gold/TiO2 core-shell nanowires were firstly prepared by the sol-gel method using a mixture of aqueous solution of titanate and chloroauric acid (HAuCI4) under ambient temperature and atmospheric condition and calcined then. As prepared nanowire was found to have a good surface biocompatibility for immobilization of the alkaline protease enzyme. The results obtained in this work showed that alkaline protease immobilized on gold-TiO2 core shell is stable and bioactive at the alkaline pH and ambient temperature. The stability and the biocatalytic activity of the immobilized gold-TiO2 core shell nano composites were monitored by UV-visible spectroscopy. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and dispersive analysis of X-ray (EDAX) were used to characterize the size and morphology of the prepared materials.
authors.affiliation: Department of Chemistry, Islamic Azad University Varamin-Pishva Branch, Varamin, 33817-74895, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Farhadyar
authors.forename: M S
authors.initials: MS
authors.lastname: Sadjadi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D010450:Endopeptidases / Q000737:chemistry*; D004800:Enzymes, Immobilized; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D053770:Nanowires / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; C009495:titanium dioxide; D006046:Gold; D014025:Titanium; D010450:Endopeptidases; C087822:alkaline protease