title: Human immunodeficiency virus type 1 Tat induces B7-H1 expression via ERK/MAPK signaling pathway.
abstract: In HIV-infected subjects, B7-H1 synthesis and expression are up-regulated, and the degree of dysregulation correlates with the severity of disease. HIV-1 Tat protein, the viral transactivating factor, represents a key target for the host immune response. However, the relationship between B7-H1 and Tat protein has not been addressed. Here, we chose human endothelial cells which provide costimulatory signals sufficiently to influence T cells. We used recombinant pcDNA3.1(+)-Tat plasmid to transfect human endothelial cells ECV304 to establish stable Tat-expressed cell strain, and found that HIV-1 Tat was able to induce B7-H1 expression in ECV304 cells by Real-time PCR and flow cytometry analysis, and inhibited lymphocyte proliferation in co-culture system. Moreover, by using pharmacological inhibitor of ERK pathway, HIV-1 Tat induces B7-H1 expression via ERK/MAPK signaling pathway was corroborated. In summary, our results indicate that HIV-1 Tat could induce B7-H1 synthesis in ECV304 cells through ERK/MAPK signaling pathway.
authors.affiliation: Institute of Molecular Biology, China Three Gorges University, Yichang, Hubei, China.
authors.forename: Jijing
authors.initials: J
authors.lastname: Shi
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qin
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Gongze
authors.initials: G
authors.lastname: Wang
authors.forename: Chaoqi
authors.initials: C
authors.lastname: Liu
mesh_terms: D060890:B7-H1 Antigen / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D018920:Coculture Techniques; D017931:DNA Primers / Q000235:genetics; D042783:Endothelial Cells / Q000276:immunology / Q000821:virology; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000473:pathology / Q000821:virology; D020935:MAP Kinase Signaling System / Q000276:immunology*; D014162:Transfection; D015854:Up-Regulation; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D017931:DNA Primers; D054322:tat Gene Products, Human Immunodeficiency Virus