title: Thyrotropin (TSH) regulates triiodothyronine (T3) production in the unicellular Tetrahymena.
abstract: The aim of the experiments was to study the regulation of triiodothyronine (T3) production in the unicellular Tetrahymena. Untreated and troph-hormone treated specimen were prepared and in different timepoints T3 content was measured and compared by immunocytochemical flow cytometry. 0.1 or 0.001 IU TSH in tryptone-yeast medium stimulated T3 synthesis at 10, 20, 30 min, but does not stimulate after 1 h. The overlapping gonadotropic hormone (GTH) also did it, however only at 10 min. In Losina salt solution (physiological for Tetrahymena) the effect was weaker, however outer amino acid source was not absolutely needed for the production of the hormone. The results show that the TSH regulation of thyroid hormone synthesis (storage, secretion) and troph-hormone overlap can be deduced to a unicellular level. This may allow the hypothesis that the endocrine mechanisms proved at a low level of phylogeny are preserved for the higher ranked organisms.
authors.affiliation: Semmelweis University Department of Genetics, Cell and Immunobiology, Budapest, Hungary. csagyor@dgci.sote.hu
authors.forename: G
authors.initials: G
authors.lastname: Csaba
authors.forename: Eva
authors.initials: E
authors.lastname: Pállinger
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods; D006062:Gonadotropins / Q000378:metabolism; D006728:Hormones / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008954:Models, Biological; D010461:Peptones / Q000494:pharmacology; D010802:Phylogeny; D013768:Tetrahymena; D013963:Thyroid Hormones / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology*; D013997:Time Factors; D014284:Triiodothyronine / Q000096:biosynthesis*
chemical_list: D006062:Gonadotropins; D006728:Hormones; D010461:Peptones; D013963:Thyroid Hormones; D014284:Triiodothyronine; C001830:tryptones; D013972:Thyrotropin