title: Melatonin ameliorates bisphenol A-induced biochemical toxicity in testicular mitochondria of mouse.
abstract: Bisphenol A (BPA) is a monomer of polycarbonate plastic used to manufacture plastic baby bottles and lining of food cans. It has endocrine-disrupting potential and exerts both toxic and estrogenic effects on mammalian cells. We studied BPA-induced perturbation of mitochondrial marker enzymes in testes of Swiss albino mice and its amelioration by melatonin. Mice exposed to standardized dose of BPA (10 mg/kg body weight) orally for 14 days showed decrease in activities of marker mitochondrial enzymes such as succinate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, monoamine oxidase and NADH dehydrogenase. Besides, it also affected activities of antioxidant enzymes such as superoxide dismutase, glutathione reductase and glutathione peroxidase. BPA also caused lipid peroxidation (LPO) and decrease in reduced glutathione (GSH) content of mitochondria. Concomitant melatonin administration (10 mg/kg body weight; intraperitoneally for 14 days) lowered mitochondrial lipid peroxidation. It also restored the activity of mitochondrial marker enzymes and ameliorated decreased enzymatic and non-enzymatic antioxidants of mitochondria. These results demonstrate that melatonin has a potential role in ameliorating BPA-induced mitochondrial toxicity and the protection is due to its antioxidant property or by the direct free radical scavenging activity.
authors.affiliation: Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
authors.forename: Sameya
authors.initials: S
authors.lastname: Anjum
authors.forename: Shakilur
authors.initials: S
authors.lastname: Rahman
authors.forename: Manpreet
authors.initials: M
authors.lastname: Kaur
authors.forename: Firoz
authors.initials: F
authors.lastname: Ahmad
authors.forename: Hina
authors.initials: H
authors.lastname: Rashid
authors.forename: Rizwan Ahmad
authors.initials: RA
authors.lastname: Ansari
authors.forename: Sheikh
authors.initials: S
authors.lastname: Raisuddin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001559:Benzhydryl Compounds; D015415:Biomarkers; D015227:Lipid Peroxidation; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D000975:Antioxidants; D001559:Benzhydryl Compounds; D015415:Biomarkers; D008550:Melatonin; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds