title: [Comparasion of polysaccharides in parent root, daughter root and rootlet of Aconitum carmichaeli].
abstract: OBJECTIVE
  To study the polysaccharides contents and monosaccharide compositions in parent root, daughter root and rootlet of Aconitum carmichaeli.
  
  
  METHODS
  The conversion coefficient of A. carmichaeli polysaccharide to glucose was obtained by refined polysaccharides, and then the contents of crude polysaccharides in parent root, daughter root and rootlet were determined by sulfuric-phenol spectrometry method; analysis of monosaccharide compositions in polysaccharides from A. carmichaeli was carried out by pre-column derivatization high performance liquid chromatography with 1-phenyl-3-methyl-5-pyrazolone (PMP).
  
  
  RESULTS
  The contents of polysaccharides in parent root, daughter root and rootlet were 22.02%, 33.53% and 6.10%, respectively. Parent root, daughter root and rootlet mainly contained glucose, and in addition they contained a small amount of galacturonic acid, galactose and arabinose. Daughter root contained mannose yet, and rootlet still contained mannose, rhamnose and xylose.
  
  
  CONCLUSIONS
  The method is simple, rapid, and accurate. The content of polysaccharide in rootlet is lowest, and monosaccharide compositions in rootlet are significantly different from parent root and daughter root.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Yonglei
authors.initials: Y
authors.lastname: Lu
authors.forename: Haibo
authors.initials: H
authors.lastname: Bu
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Xiangri
authors.initials: X
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Li
mesh_terms: D031841:Aconitum / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D011134:Polysaccharides