title: Characterization and application of the Fe(II) and α-ketoglutarate dependent hydroxylase FrbJ.
abstract: The Fe(II) and α-ketoglutarate-dependent hydroxylase FrbJ was previously demonstrated to utilize FR-900098 synthesizing a second phosphonate FR-33289. Here we assessed its ability to hydroxylate other possible substrates, generating a library of potential antimalarial compounds. Through a series of bioassays and in vitro experiments, we identified two new antimalarials.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: DeSieno
authors.forename: Wilfred A
authors.initials: WA
authors.lastname: van der Donk
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000962:Antimalarials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry*; D005578:Fosfomycin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000737:chemistry*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology
chemical_list: D000962:Antimalarials; D001426:Bacterial Proteins; D005296:Ferrous Compounds; D007656:Ketoglutaric Acids; D005578:Fosfomycin; C024640:fosmidomycin; C024639:3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid; C024642:FR 33289; D006899:Mixed Function Oxygenases