title: [Oryzalin-induced chromosome doubling of Scutellaria barbata].
abstract: OBJECTIVE
  To obtain polyploidy Scutellaria barbata.
  
  
  METHODS
  Explants of stem segment of Scutellaria barbata were treated with oryzalin at various concentration and time duration in vitro.
  
  
  RESULTS
  Comparing with the characteristics of diploid, mutant plants had significant changes in plant morphology inducing compact plant, darker leaf color, smaller leaf index, thicken stem, significantly larger stomata, guard cells and chloroplasts in stomata guard cells and significantly lower stomata density. Mutant plants were plant chimera of diploid and tetraploid assessed by chromosome counting and flow cytometry.
  
  
  CONCLUSIONS
  The technique could be used for chromosome doubling in Scutellaria barbata.
authors.affiliation: College of Life Sciences Nanjing Normal University, Jiangsu Key Laboratory for Biodiversity and Biotechnology, Nanjing 210046, China. yuemin0538@126.com
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.forename: Chong-shun
authors.initials: CS
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Liao
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Wei-jun
authors.initials: WJ
authors.lastname: Shen
mesh_terms: D032461:Chromosomes, Plant / Q000187:drug effects* / Q000235:genetics; D003433:Crosses, Genetic; D004136:Dinitrobenzenes / Q000494:pharmacology*; D004171:Diploidy; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018547:Plant Stems / Q000187:drug effects / Q000502:physiology; D011123:Polyploidy; D031363:Scutellaria / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D013424:Sulfanilamides / Q000494:pharmacology*; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D004136:Dinitrobenzenes; D013424:Sulfanilamides; C012465:oryzalin