title: A simple and effective SuperBuffer for DNA agarose electrophoresis.
abstract: In the paper, we describe a unique effective electrophoresis buffer for DNA agarose electrophoresis, called SuperBuffer. Using this buffer, electrophoresis could be performed within 10 min at voltages as high as 25V/cm. In addition, DNA fragments of different lengths could be isolated clearly even at lower agarose gel concentrations and the DNA recovery efficiency was higher than that of the TAE/TBE running buffers. The SuperBuffer still retained its electrophoretic effect even after several uses.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, People's Republic of China.
authors.forename: Jun-He
authors.initials: JH
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
mesh_terms: D000085:Acetates; D001888:Boric Acids; D002021:Buffers; D004247:DNA / Q000032:analysis*; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005029:Ethylenediamines; D005782:Gels / Q000737:chemistry; D015203:Reproducibility of Results; D012685:Sepharose / Q000737:chemistry; D013997:Time Factors; D014325:Tromethamine
chemical_list: D000085:Acetates; D001888:Boric Acids; D002021:Buffers; D005029:Ethylenediamines; D005782:Gels; C069591:Tris-borate-EDTA buffer; C115179:tris-acetate-EDTA buffer; D014325:Tromethamine; D004247:DNA; D012685:Sepharose; D004492:Edetic Acid