title: A water soluble vitamin B12-ReI fluorescent conjugate for cell uptake screens: use in the confirmation of cubilin in the lung cancer line A549.
abstract: A water soluble vitamin B(12)-rhenium conjugate was synthesized and used in concert with intrinsic factor to screen for cubilin receptor-mediated uptake in lung cancer cells. Internalization of the conjugate demonstrated that it could be used to rapidly screen for the cubilin receptor in living cells, subsequently confirmed with Western blotting and RT-PCR.
authors.affiliation: Department of Chemistry, Centre for Science and Technology, Syracuse University, Syracuse, NY 13244, USA.
authors.forename: Anthony R
authors.initials: AR
authors.lastname: Vortherms
authors.forename: Anna R
authors.initials: AR
authors.lastname: Kahkoska
authors.forename: Amy E
authors.initials: AE
authors.lastname: Rabideau
authors.forename: Jon
authors.initials: J
authors.lastname: Zubieta
authors.forename: Louise Lund
authors.initials: LL
authors.lastname: Andersen
authors.forename: Mette
authors.initials: M
authors.lastname: Madsen
authors.forename: Robert P
authors.initials: RP
authors.lastname: Doyle
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000235:genetics*; D012211:Rhenium / Q000737:chemistry*; D012995:Solubility; D014805:Vitamin B 12 / Q000737:chemistry* / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011956:Receptors, Cell Surface; C029486:intrinsic factor-cobalamin receptor; D014867:Water; D012211:Rhenium; D014805:Vitamin B 12