title: Lipopolysaccharide-induced innate immune factors in the bottlenose dolphin (Tursiops truncatus) detected in expression sequence tag analysis.
abstract: EST analysis based on the megaclone-megasorting method was performed using leukocytes from the bottlenose dolphin (Tursiops truncatus) with or without LPS stimulation. A total of 849 upregulated and 384 downregulated EST clones were sequenced, annotated, and functionally classified. Ferritin heavy peptide I was the most abundant upregulated transcript, suggesting that LPS stimulation induced high production of reactive oxygen species, which were sequestered in ferritin. Among the immune factors, the transcripts coding for an IL-1Ra, homologs to bovine serum amyloid A3, and canine intercellular adhesion molecule-1 were highly expressed. Markedly downregulated transcripts of immune factors were those for homologs of calcium-binding proteins belonging to the S100 family, S100A12, S100A8, and S100A6. Time-course experiments on the expression of some immune factors including IL-1Ra suggested that these factors interact and control cetacean innate immunity.
authors.affiliation: Marine Biodiversity Research Program, Japan Agency for Marine-Earth Science and Technology-JAMSTEC, Yokosuka, Kanagawa 237-0061, Japan. oishik@jamstec.go.jp
authors.forename: Kazue
authors.initials: K
authors.lastname: Ohishi
authors.forename: Reiko
authors.initials: R
authors.lastname: Shishido
authors.forename: Yasunao
authors.initials: Y
authors.lastname: Iwata
authors.forename: Masafumi
authors.initials: M
authors.lastname: Saitoh
authors.forename: Ryota
authors.initials: R
authors.lastname: Takenaka
authors.forename: Dai
authors.initials: D
authors.lastname: Ohtsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Okutsu
authors.forename: Tadashi
authors.initials: T
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000276:immunology*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D007155:Immunologic Factors / Q000096:biosynthesis* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*
chemical_list: D007155:Immunologic Factors; D008070:Lipopolysaccharides