title: Direct radiolabelling of proteins at cysteine using [18F]-fluorosugars.
abstract: A strategy for the site-specific attachment of 2-deoxy-2-fluorosugars to cysteine and dehydroalanine tagged proteins is reported. When combined with thionation of fluorosugars, such as the widely available (18)F probe 2-deoxy-2-[(18)F]fluoroglucose ([(18)F]FDG), this methodology allows fast and direct access to site-specific [(18)F]FDG-labelled proteins.
authors.affiliation: Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK.
authors.forename: Omar
authors.initials: O
authors.lastname: Boutureira
authors.forename: Gonçalo J L
authors.initials: GJ
authors.lastname: Bernardes
authors.forename: François
authors.initials: F
authors.lastname: D'Hooge
authors.forename: Benjamin G
authors.initials: BG
authors.lastname: Davis
mesh_terms: D003545:Cysteine / Q000737:chemistry; D019788:Fluorodeoxyglucose F18 / Q000737:chemistry*; D007553:Isotope Labeling; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D049268:Positron-Emission Tomography; D011506:Proteins / Q000737:chemistry*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry*; D020860:Subtilisin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D011506:Proteins; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D020860:Subtilisin; D003545:Cysteine