title: Activation of STAT1 in neurons following spinal cord injury in mice.
abstract: The signal transducer and activator of transcription 1 (STAT1) has been reported to be associated with neuronal cell death after cerebral ischemia. On the contrary, STAT3 has been revealed to regulate cell survival. We examined the chronological alteration and cellular localization of phosphorylated (p)-JAK1, p-STAT1 and p-STAT3 following mild spinal cord injury (SCI) in mice. Western blot analysis indicated that JAK1 is significantly phosphorylated, accompanied by the phosphorylation of STAT1 at Tyr(701) within a similar timeframe. Immunofluorescence staining indicated that signal transduction of STAT3 is introduced into the nucleus of the neurons within the anterior horns; however, in mirror sections, that of STAT1 is limited to the cytoplasm. These findings suggest that STAT3 signal is predominantly transduced into the nucleus and plays a stronger role in neuronal survival than STAT1. Modulation of the functional balance between STAT1 and STAT3 might determine the survival or death of neurons after SCI.
authors.affiliation: Department of Neurological Surgery, Aichi Medical University, 21 Yazako, Nagakute, Aichi 480-1195, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Osuka
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Nobuteru
authors.initials: N
authors.lastname: Usuda
authors.forename: Kimie
authors.initials: K
authors.lastname: Atsuzawa
authors.forename: Muneyoshi
authors.initials: M
authors.lastname: Yasuda
authors.forename: Chihiro
authors.initials: C
authors.lastname: Aoshima
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: Masakazu
authors.initials: M
authors.lastname: Takayasu
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005260:Female; D053613:Janus Kinase 1 / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D050794:STAT1 Transcription Factor / Q000378:metabolism*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; D014443:Tyrosine; D053613:Janus Kinase 1