title: The streptozotocin-induced rat model of diabetes mellitus evidences significant reduction of myosin-Va expression in the brain.
abstract: Diabetes mellitus is a disease characterized by increased glucose levels in the blood. Hyperglycemia causes damage to the brain tissue, and induces significant changes in synaptic transmission. In this investigation, we have found a significant alteration in the expression of the molecular motor involved in the synaptic vesicles transport, myosin-Va, and its distribution in rat brains of streptozotocin-induced diabetes model. Brains were removed after 20 days, homogenized and analysed by Western blotting, qRT-PCR and immunohistochemistry. Myosin-Va presented significantly lower levels of both mRNA and protein in diabetic than those observed in non-diabetic animals. Moreover, neuronal and glial cells of the occipital and frontal cortex exhibited decreased myosin-Va immunostaining in diabetic rat brains. In conclusion, diabetic rat brains displayed altered expression and distribution of myosin-Va, and these finding may contribute to the basic understanding about this myosin role in brain function related to diabetes.
authors.affiliation: Institute of Genetics and Biochemistry, Federal University of Uberlândia, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil. alice_udi@hotmail.com
authors.forename: Alice Vieira
authors.initials: AV
authors.lastname: da Costa
authors.forename: Luciana Karen
authors.initials: LK
authors.lastname: Calábria
authors.forename: Rafael
authors.initials: R
authors.lastname: Nascimento
authors.forename: Washington João
authors.initials: WJ
authors.lastname: Carvalho
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
authors.forename: Foued Salmen
authors.initials: FS
authors.lastname: Espindola
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D004195:Disease Models, Animal; D005625:Frontal Lobe / Q000166:cytology / Q000378:metabolism; D006943:Hyperglycemia / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D024701:Myosin Type V / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009778:Occipital Lobe / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013311:Streptozocin / Q000009:adverse effects; D013572:Synaptic Vesicles / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D013311:Streptozocin; D024701:Myosin Type V