title: Survival of probiotic lactobacilli in the upper gastrointestinal tract using an in vitro gastric model of digestion.
abstract: The aim of this study was to investigate survival of three commercial probiotic strains (Lactobacillus casei subsp. shirota, L. casei subsp. immunitas, Lactobacillus acidophilus subsp. johnsonii) in the human upper gastrointestinal (GI) tract using a dynamic gastric model (DGM) of digestion followed by incubation under duodenal conditions. Water and milk were used as food matrices and survival was evaluated in both logarithmic and stationary phase. The % of recovery in logarithmic phase ranged from 1.0% to 43.8% in water for all tested strains, and from 80.5% to 197% in milk. Higher survival was observed in stationary phase for all strains. L. acidophilus subsp. johnsonii showed the highest survival rate in both water (93.9%) and milk (202.4%). Lactic acid production was higher in stationary phase, L. casei subsp. shirota producing the highest concentration (98.2 mM) after in vitro gastric plus duodenal digestion.
authors.affiliation: Department of Food and Environmental Sciences, University of Messina, 98166 S. Agata di Messina, Messina, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Lo Curto
authors.forename: Iole
authors.initials: I
authors.lastname: Pitino
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Mandalari
authors.forename: Jack Richard
authors.initials: JR
authors.lastname: Dainty
authors.forename: Richard Martin
authors.initials: RM
authors.lastname: Faulks
authors.forename: Martin Sean
authors.initials: MS
authors.lastname: John Wickham
mesh_terms: D004063:Digestion; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development* / Q000378:metabolism; D019936:Probiotics; D041742:Upper Gastrointestinal Tract / Q000382:microbiology*
chemical_list: D019344:Lactic Acid