title: Substituted 2-aminothiophenes: antifungal activities and effect on Microsporum gypseum protein profile.
abstract: The increasing recognition and importance of fungal infections, the difficulties encountered in their treatment and the increase in resistance to antifungal agents have stimulated the search for therapeutic alternatives. The objective of this study was to evaluate the antifungal activities of three substituted 2-aminothiophenes (1, 2 and 3) against some fungal species. The synthesis of substituted 2-aminothiophenes was carried out through the most versatile synthetic method developed by Gewald et al. The antifungal activity was performed against yeast, dermatophytes and Aspergillus species using the broth microdilution method. The effect of these aminothiophenes was examined on the protein content and profile. Compound 2 was the most active (MIC varying from 2.00 to 128 μg ml(-1) ). All the three substituted 2-aminothiophenes had a relatively important dose-dependent effect on Microsporum gypseum protein profile and content. These compounds affected the structure and dye fixation of macroconidia of this fungus. The overall results indicate that the tested substituted 2-aminothiophenes can be used as precursors for new antifungal drugs development.
authors.affiliation: Laboratory of Microbiology and Antimicrobial Substances, Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
authors.forename: Pythagore Soubgwi
authors.initials: PS
authors.lastname: Fogue
authors.forename: Paul Keilah
authors.initials: PK
authors.lastname: Lunga
authors.forename: Emmanuel Sopbué
authors.initials: ES
authors.lastname: Fondjo
authors.forename: Jean
authors.initials: J
authors.lastname: De Dieu Tamokou
authors.forename: Boudjeko
authors.initials: B
authors.lastname: Thaddée
authors.forename: Joseph
authors.initials: J
authors.lastname: Tsemeugne
authors.forename: Amandine Tienga
authors.initials: AT
authors.lastname: Tchapi
authors.forename: Jules-Roger
authors.initials: JR
authors.lastname: Kuiate
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects* / Q000378:metabolism*; D020543:Proteome; D013876:Thiophenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D020543:Proteome; D013876:Thiophenes