title: IL-22 producing NKp46+ innate lymphoid cells can differentiate from hematopoietic precursor cells.
abstract: The IL-22 NKp46(+) innate lymphoid cells, NCR22 cells, are very important for the early host defense against microbial pathogens. We show here that NCR22 cells were differentiated from Lin(-)CD127(+)CD117(+) cells that were derived from hematopoietic precursor cells (HPCs) of mouse bone marrow cells. The combination of low concentrations of IL-23 and IL-15 induced differentiation of NCR22 cells from Lin(-)CD127(+)CD117(+) cells. NCR22 cells expressed a large amount of IL-22 and RORγt, and they had poor cytolytic activity and produced little IFN-γ. Lin(-)CD127(+)CD117(+) cells were very similar to intestinal lamina propria LTi-like cells; both cells dominantly expressed RORγt and IL-22. Meanwhile, Lin(-)CD127(-)CD117(+) cells that were also derived from HPCs did not express RORγt and IL-22, and they developed into conventional NK cells, not into NCR22 cells. These findings revealed that NCR22 cells can be differentiated from Lin(-)CD127(+)CD117(+) cells which are derived from HPCs.
authors.affiliation: Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Republic of Korea.
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Kim
authors.forename: Won Sam
authors.initials: WS
authors.lastname: Kim
authors.forename: Zheng-Hao
authors.initials: ZH
authors.lastname: Piao
authors.forename: Sohyun
authors.initials: S
authors.lastname: Yun
authors.forename: Suk Hyung
authors.initials: SH
authors.lastname: Lee
authors.forename: Suui
authors.initials: S
authors.lastname: Lee
authors.forename: Mira
authors.initials: M
authors.lastname: Jeong
authors.forename: Hu-Nan
authors.initials: HN
authors.lastname: Sun
authors.forename: Young-Jun
authors.initials: YJ
authors.lastname: Park
authors.forename: Haiyoung
authors.initials: H
authors.lastname: Jung
authors.forename: Suk Ran
authors.initials: SR
authors.lastname: Yoon
authors.forename: Inpyo
authors.initials: I
authors.lastname: Choi
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D019409:Interleukin-15 / Q000276:immunology / Q000378:metabolism; D053759:Interleukin-23 / Q000378:metabolism; D053699:Interleukin-7 Receptor alpha Subunit / Q000378:metabolism; D007378:Interleukins / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000378:metabolism; D007694:Killer Cells, Natural / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D055627:Natural Cytotoxicity Triggering Receptor 1 / Q000378:metabolism*; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000096:biosynthesis*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D000950:Antigens, Ly; D019409:Interleukin-15; D053759:Interleukin-23; D053699:Interleukin-7 Receptor alpha Subunit; D007378:Interleukins; D055627:Natural Cytotoxicity Triggering Receptor 1; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D019009:Proto-Oncogene Proteins c-kit; D000097043:Interleukin-22; C118384:Ncr1 protein, mouse