title: Nuclear lamins are differentially expressed in retinal neurons of the adult rat retina.
abstract: Lamins are type V intermediate filament proteins that support nuclear membranes. They are divided into A-type lamins, which include lamin A and C, and B-type lamins, which include lamin B1 and B2. In the rat brain, lamin A and C are expressed in relatively equal amounts, while the expressions of lamin B1 and B2 vary depending on the cell type. Lamins play important roles in normal morphogenesis and function. In the nervous system, their abnormal expression causes several neurodegenerative diseases such as peripheral neuropathy, leukodystrophy and lissencephaly. The retina belongs to the central nervous system (CNS) and has widely been used as a source of CNS neurons. We investigated the expression patterns of lamin subtypes in the adult rat retina by immunohistochemistry and found that the staining patterns differed when compared with the brain. All retinal neurons expressed lamin B1 and B2 in relatively equal amounts. In addition, horizontal cells and a subpopulation of retinal ganglion cells expressed lamin A and C, while photoreceptor cells expressed neither lamin A nor C, and all other retinal neurons expressed lamin C only. This differential expression pattern of lamins in retinal neurons suggests that they may be involved in cellular differentiation and expression of cell-specific genes in individual retinal neurons.
authors.affiliation: Department of Anatomy and Cell Science, Kansai Medical University, Moriguchi, Osaka, Japan. wakabayt@takii.kmu.ac.jp
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Wakabayashi
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Mori
authors.forename: Yukie
authors.initials: Y
authors.lastname: Hirahara
authors.forename: Taro
authors.initials: T
authors.lastname: Koike
authors.forename: Yumene
authors.initials: Y
authors.lastname: Kubota
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Takamori
authors.forename: Hisao
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D007150:Immunohistochemistry; D034882:Lamins / Q000096:biosynthesis* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000378:metabolism*
chemical_list: D034882:Lamins