title: Gene expression profile of steroid-induced necrosis of femoral head of rats.
abstract: The key to treating steroid-induced necrosis of femoral heads (SINFH) is early diagnosis. Dramatic improvements in diagnosis could be made if the pathogenesis of SINFH was more fully understood; however, the underlying mechanism of this disease is currently unknown. To explore the potential mechanism of SINFH, we performed gene array analysis on a rat model of the disease and compare the expression profile with that of normal rats. A quantitative RT-PCR and immunohistochemistry (IHC) assays were used to confirm the microarray results. Compared to the control group, 190 genes in the experimental group were differentially expressed, with 52 up-regulated and 138 down-regulated. Of these genes, 102 are known (deposited in GenBank), while 88 of them are unknown. The known genes can be divided into several families according to their biological functions, such as oxidative stress, apoptosis, signal transduction, angiogenesis, extracellular matrix, lipid metabolism, and transcription related genes. The results of quantitative RT-PCR and IHC were consistent with gene chip results. Our findings indicate that many genes involved in diverse signaling pathways were differentially expressed between SINFH rats and normal rats. Furthermore, our findings suggest that the development of SINFH is a complicated and dynamic process affected by multiple factors and signaling pathways and regulated by various genes.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
authors.forename: Peijian
authors.initials: P
authors.lastname: Tong
authors.forename: Chengliang
authors.initials: C
authors.lastname: Wu
authors.forename: Hongting
authors.initials: H
authors.lastname: Jin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Mao
authors.forename: Nanze
authors.initials: N
authors.lastname: Yu
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Holz
authors.forename: Letian
authors.initials: L
authors.lastname: Shan
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Luwei
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D016000:Cluster Analysis; D004195:Disease Models, Animal; D005271:Femur Head Necrosis / Q000139:chemically induced* / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D020869:Gene Expression Profiling; D005938:Glucocorticoids / Q000009:adverse effects; D008297:Male; D046228:Microarray Analysis; D051381:Rats; D017208:Rats, Wistar; D013256:Steroids / Q000009:adverse effects*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005938:Glucocorticoids; D013256:Steroids