title: Nickel-quinolones interaction. Part 5-Biological evaluation of nickel(II) complexes with first-, second- and third-generation quinolones.
abstract: The nickel(II) complexes with the quinolone antibacterial agents oxolinic acid, flumequine, enrofloxacin and sparfloxacin in the presence of the N,N'-donor heterocyclic ligand 2,2'-bipyridylamine have been synthesized and characterized. The quinolones act as bidentate ligands coordinated to Ni(II) ion through the pyridone oxygen and a carboxylato oxygen. The crystal structure of [(2,2'-bipyridylamine)bis(sparfloxacinato)nickel(II)] has been determined by X-ray crystallography. UV study of the interaction of the complexes with calf-thymus DNA (CT DNA) has shown that they bind to CT DNA with [(2,2'-bipyridylamine)bis(flumequinato)nickel(II)] exhibiting the highest binding constant to CT DNA. The cyclic voltammograms of the complexes have shown that in the presence of CT DNA the complexes can bind to CT DNA by the intercalative binding mode which has also been verified by DNA solution viscosity measurements. Competitive study with ethidium bromide (EB) has shown that the complexes can displace the DNA-bound EB indicating that they bind to DNA in strong competition with EB. The complexes exhibit good binding propensity to human or bovine serum albumin protein having relatively high binding constant values. The biological properties of the [Ni(quinolonato)(2)(2,2'-bipyridylamine)] complexes have been evaluated in comparison to the previously reported Ni(II) quinolone complexes [Ni(quinolonato)(2)(H(2)O)(2)], [Ni(quinolonato)(2)(2,2'-bipyridine)] and [Ni(quinolonato)(2)(1,10-phenanthroline)]. The quinolones and their Ni(II) complexes have been tested for their antioxidant and free radical scavenging activity. They have been also tested in vitro for their inhibitory activity against soybean lipoxygenase.
authors.affiliation: Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Kalliopi C
authors.initials: KC
authors.lastname: Skyrianou
authors.forename: Franc
authors.initials: F
authors.lastname: Perdih
authors.forename: Athanasios N
authors.initials: AN
authors.lastname: Papadopoulos
authors.forename: Iztok
authors.initials: I
authors.lastname: Turel
authors.forename: Dimitris P
authors.initials: DP
authors.lastname: Kessissoglou
authors.forename: George
authors.initials: G
authors.lastname: Psomas
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry; D024841:Fluoroquinolones / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009532:Nickel / Q000737:chemistry*; D010093:Oxolinic Acid / Q000737:chemistry; D015363:Quinolones / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D024841:Fluoroquinolones; D015363:Quinolones; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D009532:Nickel; D004247:DNA; C427203:calf thymus DNA; D010093:Oxolinic Acid; C061363:sparfloxacin; C012976:flumequine