title: A simple and rapid technique for the authentication of the ginseng cultivar, Yunpoong, using an SNP marker in a large sample of ginseng leaves.
abstract: Yunpoong is an important Korean ginseng (Panax ginseng C. A. Meyer) cultivar, but no molecular marker has been available to identify Yunpoong from other cultivars. In this study, we developed a single nucleotide polymorphism (SNP) marker for Yunpoong based on analysis of expressed sequence tags (ESTs) in an exon region of the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. This SNP marker had high specificity to authenticate Yunpoong in twelve different main ginseng cultivars. For application of the molecular marker, a rapid identification method was established based on the NaOH-Tris method and real-time polymerase chain reaction (PCR) in order to ensure more efficiency in the cultivar selection. The biggest feature of the NaOH-Tris method was that it made the extraction of DNA very simple and rapid in young leaf tissues. We only spent 1 min to extract DNA and directly used it to do PCR. In this report, the conventional DNA extraction method was used to develop molecular marker process, and the NaOH-Tris method was applied in screening large numbers of cultivars. Moreover, the greatest advantage of the real-time PCR compared with traditional PCR, is time saving and high efficiency. Thus, this strategy provides a rapid and reliable method for the specific identification of Yunpoong in a large number of samples.
authors.affiliation: Department of Oriental Medicinal Material & Processing, Kyung Hee University, Yongin-si, Republic of Korea.
authors.forename: Hua
authors.initials: H
authors.lastname: Sun
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Wang
authors.forename: Woo-Saeng
authors.initials: WS
authors.lastname: Kwon
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jun-Gyo
authors.initials: JG
authors.lastname: In
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
mesh_terms: D018744:DNA, Plant / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005894:Panax / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012972:Sodium Hydroxide / Q000737:chemistry; D013045:Species Specificity; D013997:Time Factors; D014325:Tromethamine
chemical_list: D018744:DNA, Plant; D014325:Tromethamine; D012972:Sodium Hydroxide