title: Structural and dynamical characterization of unilamellar AOT vesicles in aqueous solutions and their efficacy as potential drug delivery vehicle.
abstract: Sodium bis(2-ethylhexyl) sulfosuccinate (AOT) is well known to form nanometre sized aqueous droplets in organic solvents and used in several contemporary applications including templates of nanoparticle synthesis. However, the detailed structural characterization of AOT in aqueous media is relatively less attended. Here we have used dynamic light scattering technique for the structural characterization of AOT in aqueous solutions and found to have a monodispersed, unilamellar vesicles (∼140 nm diameter). The efficacy of the vesicle to host both charged drugs like H258 (2'-(4-hydroxyphenyl)-5-[5-(4-methylpiperazine-1-yl)-benzimidazo-2-yl-benzimidazole]), EtBr (ethidium bromide) and hydrophobic drug like DCM (4-(dicyanomethylene)-2-methyl-6-(p-dimethylamino-styryl)-4H-pyran) has also been investigated using Förster resonance energy transfer. Picosecond resolved and polarization gated spectroscopy have been used to study the solvation dynamics and microviscosity at the surface of the vesicles. We have also performed concentration and temperature dependent studies in order to confirm the stability of the vesicles in aqueous phase. The drug release profile of the vesicles has been studied through in vitro dialysis method. The non-toxic, monodispersed vesicles in aqueous media with a noteworthy stability in wide range of AOT concentration and temperature, capable of hosting drugs of various natures (both hydrophobic and charged) simultaneously for many codelivery applications with controlled drug release profile may find its applications in drug delivery.
authors.affiliation: Department of Chemical, Biological & Macromolecular Sciences, SN Bose National Centre for Basic Sciences, Salt Lake, Kolkata, India.
authors.forename: Ranajay
authors.initials: R
authors.lastname: Saha
authors.forename: Pramod Kumar
authors.initials: PK
authors.lastname: Verma
authors.forename: Rajib Kumar
authors.initials: RK
authors.lastname: Mitra
authors.forename: Samir Kumar
authors.initials: SK
authors.lastname: Pal
mesh_terms: D004337:Drug Carriers / Q000737:chemistry*; D008956:Models, Chemical; D013386:Succinates / Q000737:chemistry*; D013696:Temperature; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: C512554:1,4-bis(2-ethylhexyl) sodium sulfosuccinate; D004337:Drug Carriers; D013386:Succinates; D053835:Unilamellar Liposomes