title: [The establishment of craniopharyngioma cell line].
abstract: OBJECTIVE
  To establish the craniopharyngioma cell line with primary culture which might provide experiment background and evidence for future eternal tumor cell line establishment.
  
  
  METHODS
  Thirty six surgical specimens were collected from patients with craniopharyngiomas definited by iconography and pathology examinations in West China Hospital, Sichuan University, including twenty one adamantine epitheliomas and fifteen squamous papillary tumors. The tumor cell was treated through primary cukture, purification, passage, freezing, resuscitation, and identified by keratin 7 staining through SP method. The growth curve and double time were detected through trypan blue dye cell count and MTT assay. The growth of the tumor cells treated with growth hormone (GH) and Tamoxifen was also observed.
  
  
  RESULTS
  Thirty six primary cultures were done, 29 of which were successful and subculture was achieved in 80.6% of all primary cultures. These cell lines were from squamous epithelium by keratin 7 antibody identification, with three days of double time. Proloferative effect of GH was most prevalent at 100 ng/mL, while tamoxifen suppressed cell growth.
  
  
  CONCLUSIONS
  The finite craniopharyngioma cell lines were obtained through primary culture.
authors.affiliation: Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Zheng-Xi
authors.initials: ZX
authors.lastname: Rao
authors.forename: Liang-Xue
authors.initials: LX
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Qiang
authors.forename: Xiu-Tian
authors.initials: XT
authors.lastname: Sima
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: You
mesh_terms: D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002648:Child; D003397:Craniopharyngioma / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000473:pathology*; D055815:Young Adult