title: Genetic, proteomic and metabolic analysis of the regulation of energy storage in rice seedlings in response to drought.
abstract: We used proteomic analysis to determine the response of rice plant seedlings to drought-induced stress. The expression of 71 protein spots was significantly altered, and 60 spots were successfully identified. The greatest down-regulated protein functional category was translation. Up-regulated proteins were mainly related to protein folding and assembly. Additionally, many proteins involved in metabolism (e.g. carbohydrate metabolism) also showed differences in expression. cDNA microarray and GC-MS analysis showed 4756 differentially expressed mRNAs and 37 differentially expressed metabolites. Once these data were integrated with the proteomic analysis, we were able to elucidate the metabolic pathways affected by drought-induced stress. These results suggest that increased energy consumption from storage substances occurred during drought. In addition, increased expression of the enzymes involved in anabolic pathways corresponded with an increase in the content of six amino acids. We speculated that energy conversion from carbohydrates and/or fatty acids to amino acids was increased. Analysis of basic metabolism networks allowed us to understand how rice plants adjust to drought conditions.
authors.affiliation: Shanghai Agrobiological Gene Center, Shanghai, PR China.
authors.forename: Liebo
authors.initials: L
authors.lastname: Shu
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Lou
authors.forename: Chenfei
authors.initials: C
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Ding
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.forename: Jinhong
authors.initials: J
authors.lastname: Wu
authors.forename: Fangjun
authors.initials: F
authors.lastname: Feng
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Lijun
authors.initials: L
authors.lastname: Luo
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
authors.forename: Hanwei
authors.initials: H
authors.lastname: Mei
mesh_terms: D055864:Droughts; D004734:Energy Metabolism; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D036226:Seedlings / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins