title: Afferent lymph-derived T cells and DCs use different chemokine receptor CCR7-dependent routes for entry into the lymph node and intranodal migration.
abstract: Little is known about the molecular mechanisms that determine the entry into the lymph node and intranodal positioning of lymph-derived cells. By injecting cells directly into afferent lymph vessels of popliteal lymph nodes, we demonstrate that lymph-derived T cells entered lymph-node parenchyma mainly from peripheral medullary sinuses, whereas dendritic cells (DCs) transmigrated through the floor of the subcapsular sinus on the afferent side. Transmigrating DCs induced local changes that allowed the concomitant entry of T cells at these sites. Signals mediated by the chemokine receptor CCR7 were absolutely required for the directional migration of both DCs and T cells into the T cell zone but were dispensable for the parenchymal entry of lymph-derived T cells and dendrite probing of DCs. Our findings provide insight into the molecular and structural requirements for the entry into lymph nodes and intranodal migration of lymph-derived cells of the immune system.
authors.affiliation: Institute of Immunology, Hannover Medical School, Hannover, Germany.
authors.forename: Asolina
authors.initials: A
authors.lastname: Braun
authors.forename: Tim
authors.initials: T
authors.lastname: Worbs
authors.forename: G Leandros
authors.initials: GL
authors.lastname: Moschovakis
authors.forename: Stephan
authors.initials: S
authors.lastname: Halle
authors.forename: Katharina
authors.initials: K
authors.lastname: Hoffmann
authors.forename: Jasmin
authors.initials: J
authors.lastname: Bölter
authors.forename: Anika
authors.initials: A
authors.lastname: Münk
authors.forename: Reinhold
authors.initials: R
authors.lastname: Förster
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D019742:Chemokines, CC / Q000276:immunology* / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007272:Injections, Intralymphatic; D008196:Lymph / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D042601:Lymphatic Vessels / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D054400:Receptors, CCR7 / Q000172:deficiency / Q000235:genetics / Q000276:immunology*; D057688:Transcellular Cell Migration / Q000276:immunology*; D057705:Transendothelial and Transepithelial Migration / Q000276:immunology*
chemical_list: D019742:Chemokines, CC; D054400:Receptors, CCR7