title: Evaluation of gene expression in MG63 human osteoblastlike cells exposed to tantalum powder by microarray technology.
abstract: Conventional orthopedic implants are composed from titanium. To improve some characteristics (ie, volumetric porosity, modulus of elasticity, frictional modulus), a new porous tantalum biomaterial has been developed and its biocompatibility reported. By using DNA microarrays containing 20,000 genes, several genes whose expression were significantly up- or down-regulated were identified in an osteoblastlike cell line (MG63) cultured with tantalum powder (TP). The differentially expressed genes cover a broad range of functional activities: signaling transduction; transcription; cell cycle regulation, proliferation, and apoptosis; and cytoskeleton formation. To the authors' knowledge, the data reported represent the first genetic portrait of TP.
authors.affiliation: Orthopedic Clinic, University of Ferrara, Ferrara, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Sollazzo
authors.forename: Furio
authors.initials: F
authors.lastname: Pezzetti
authors.forename: Leo
authors.initials: L
authors.lastname: Massari
authors.forename: Annalisa
authors.initials: A
authors.lastname: Palmieri
authors.forename: Giorgio
authors.initials: G
authors.lastname: Brunelli
authors.forename: Ilaria
authors.initials: I
authors.lastname: Zollino
authors.forename: Alessandra
authors.initials: A
authors.lastname: Lucchese
authors.forename: Gaetano
authors.initials: G
authors.lastname: Caruso
authors.forename: Francesco
authors.initials: F
authors.lastname: Carinci
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003599:Cytoskeleton / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D055119:Elastic Modulus; D017276:Friction; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008422:Materials Testing; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010006:Osteoblasts / Q000187:drug effects*; D016062:Porosity; D011208:Powders; D015398:Signal Transduction / Q000235:genetics; D013635:Tantalum / Q000737:chemistry / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D001672:Biocompatible Materials; D011208:Powders; D013635:Tantalum