title: Validation of an osteoporotic animal model for dental implant analyses: an in vivo densitometric study in rabbits.
abstract: OBJECTIVE
  The achievement of primary stability in porous and soft bone, where implants are more likely to fail, is one of the unresolved challenges of implant dentistry. Therefore, the aim of the study was to validate an osteoporotic animal model for analysis of poor-quality bone.
  
  
  METHODS
  Sixteen female New Zealand rabbits, each 6 months old and weighing 4 to 5 kg, were used in this study. The animals were anesthetized, and an in vivo densitometric analysis was performed by dual-energy x-ray absorptiometry (DEXA) to measure bone mineral density (BMD) in the calvaria, cervical spine, and tibia. Ovariectomy was then performed, and animals were fed a low-calcium diet that featured 0.07% calcium, rather than the 0.45% calcium of a standard diet, for 6 weeks. After this period, new densitometric measurements were carried out. Two-way analysis of variance was used for statistical evaluation. A P value of less than .05 was considered to be significant.
  
  
  RESULTS
  Together, ovariectomy and a low-calcium diet were able to induce a quick decrease in BMD, as measured at 6 weeks by DEXA. This decrease was statistically significant in the calvaria (P < .001) and the cervical spine (P < .05) but not in the tibia.
  
  
  CONCLUSIONS
  Based upon this study, ovariectomy and a low-calcium diet are able to induce experimental osteoporosis in rabbits in a short period of time.
authors.affiliation: Department of Health Sciences III, Health Sciences School, Rey Juan Carlos University, Alcorcón, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Martin-Monge
authors.forename: Isabel F
authors.initials: IF
authors.lastname: Tresguerres
authors.forename: Luis
authors.initials: L
authors.lastname: Blanco
authors.forename: Ameen
authors.initials: A
authors.lastname: Khraisat
authors.forename: Rosa
authors.initials: R
authors.lastname: Rodríguez-Torres
authors.forename: Jesús A F
authors.initials: JA
authors.lastname: Tresguerres
mesh_terms: D015502:Absorptiometry, Photon; D000818:Animals; D015519:Bone Density; D002118:Calcium / Q000172:deficiency; D002574:Cervical Vertebrae / Q000601:surgery; D003758:Dental Implantation, Endosseous; D005260:Female; D020007:Implants, Experimental; D023421:Models, Animal; D010024:Osteoporosis / Q000601:surgery; D010052:Ovariectomy; D011817:Rabbits; D015203:Reproducibility of Results; D012886:Skull / Q000601:surgery; D013977:Tibia / Q000601:surgery
chemical_list: D002118:Calcium