title: Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy.
abstract: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two.
  
  
  
  To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples.
  
  
  
  Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normotensive controls on their hospital admission at term for cesarean delivery. Placental biopsy was obtained after aseptic placental collection at the time of elective cesarean delivery. Subgingival plaque and placental biopsy were studied for Porphyromonas gingivalis, Fusobacterium nucleatum, Treponema denticola, Prevotella intermedia and Aggregatibacter actinomycetemcomitans using quantitative polymerase chain reaction technique.                         Periodontist and laboratory personnel were unaware of case or control status. Periodontal status was not informed to the obstetrician recruiting the cases and laboratory. Microbiology report was not revealed till end of the study.
  
  
  
  Periodontal pathogens were found to be high in the group with hypertension than the controls. P gingivalis was found in all the samples from subgingival plaque and placenta, irrespective of the periodontal disease status.
  
  
  
  In cases with hypertension, periodontal pathogens are present in higher proportion in subgingival plaque and placenta.
authors.affiliation: Department of Periodontics, Manipal College of Dental Sciences, Manipal 576104, India. swatipralhad@gmail.com
authors.forename: P
authors.initials: P
authors.lastname: Swati
authors.forename: K
authors.lastname: Ambika Devi
authors.forename: Betsy
authors.initials: B
authors.lastname: Thomas
authors.forename: Saadi Abdul
authors.initials: SA
authors.lastname: Vahab
authors.forename: Satyamoorthy
authors.initials: S
authors.lastname: Kapaettu
authors.forename: Pralhad
authors.initials: P
authors.lastname: Kushtagi
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D002585:Cesarean Section; D003773:Dental Plaque / Q000382:microbiology*; D005260:Female; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D005891:Gingivitis / Q000382:microbiology*; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000382:microbiology*; D010510:Periodontal Diseases / Q000382:microbiology*; D010922:Placenta Diseases / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D011247:Pregnancy; D018713:Prevotella intermedia / Q000302:isolation & purification; D048373:Treponema denticola / Q000302:isolation & purification; D055815:Young Adult