title: Identification of sulfoquinovosyldiacyglycerides from Phaeodactylum tricornutum by matrix-assisted laser desorption/ionization QTrap time-of-flight hybrid mass spectrometry.
abstract: The pharmaceutical industry is interested in identifying novel target compounds. Due to their versatile pharmacological activities (e.g. antiviral, anti-carcinogen and immunosuppressive) sulfoquinovosyldiacylglycerides (SQDGs) are potential drug candidates. The present publication deals with the purification and structural characterization of SQDGs from three different strains of Phaeodactylum tricornutum. Besides detection of SQDGs (sn-1: C16:1/sn-2: C16:0 and sn-1: C20:5/sn-2: C16:0), two novel 2'-O-acylsulfoquinovosyldiacylglyerides (Ac-SQDGs, sn-1: C16:0/ sn-2: C16:0/2' C20:5 and sn-1: C20:5/sn-2: C16:0/2' C20:5) were identified by using matrix-assisted laser desorption/ionization (MALDI) QTrap time-of-flight (ToF) hybrid mass spectrometry (MS) with multistage MS(n). The analytical method enables the sn-position verification of fatty acids (MS(2)) as well as the confirmation of the regioposition of eicospentanoic acid at the sulfoquinovose (MS(3)).
authors.affiliation: Institute of Bioprocess Engineering, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Naumann
authors.forename: Barbara C
authors.initials: BC
authors.lastname: Klein
authors.forename: Sebastian J
authors.initials: SJ
authors.lastname: Bartel
authors.forename: Kai H
authors.initials: KH
authors.lastname: Darsow
authors.forename: Rainer
authors.initials: R
authors.lastname: Buchholz
authors.forename: Harald A
authors.initials: HA
authors.lastname: Lange
mesh_terms: D017377:Diatoms / Q000737:chemistry* / Q000378:metabolism; D004075:Diglycerides / Q000737:chemistry* / Q000302:isolation & purification; D008757:Methylglucosides / Q000737:chemistry*; D052616:Solid Phase Extraction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D004075:Diglycerides; D008757:Methylglucosides; C009358:sulfoquinovose