title: Quantum dot-labeled aptamer nanoprobes specifically targeting glioma cells.
abstract: Two new techniques, aptamer-based specific recognition and quantum dot (QD)-based fluorescence labeling, are becoming increasingly important in biosensing. In this study, these two techniques have been coupled together to construct a new kind of fluorescent QD-labeled aptamer (QD-Apt) nanoprobe by conjugating GBI-10 aptamer to the QD surface. GBI-10 is a single-stranded DNA (ssDNA) aptamer for tenascin-C, which distributes on the surface of glioma cells as a dominant extracellular matrix protein. The QD-Apt nanoprobe can recognize the tenascin-C on the human glioma cell surface, which will be helpful for the development of new convenient and sensitive in vitro diagnostic assays for glioma. The QD-Apt nanoprobe has particular features such as strong fluorescence, stability, monodispersity and uniformity. In addition, this probe preparation method is universal, so it is expected to provide a new type of stable nanoprobe for high-throughput and fast biosensing detection and bioimaging. New methods for real-time and dynamic tracking and imaging can be accordingly developed.
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, People's Republic of China.
authors.forename: Xue-Chai
authors.initials: XC
authors.lastname: Chen
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.forename: Dai-Wen
authors.initials: DW
authors.lastname: Pang
authors.forename: Hong
authors.initials: H
authors.lastname: Qing
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xie