title: A comparison of Tris-glycine and Tris-tricine buffers for the electrophoretic separation of major serum proteins.
abstract: This paper compares different buffer systems for the electrophoretic separation of the five most abundant serum proteins on native-PAGE gel and cellulose membranes. A modified Tris-tricine system was shown to be superior for the separation of these serum proteins in a 7% m/v native-PAGE gel as compared with the traditionally used Tris-glycine and Tris-tricine methods. This modified Tris-tricine buffer system was also employed for the separation of serum proteins using a cellulose acetate membrane and very effective separation was observed as compared with the traditionally used Tris-barbital and Tris-glycine buffer systems.
authors.affiliation: Centre for Analytical Science, Department of Chemistry, Loughborough University, Leicestershire, UK.
authors.forename: Syed R
authors.initials: SR
authors.lastname: Haider
authors.forename: Barry L
authors.initials: BL
authors.lastname: Sharp
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Reid
mesh_terms: D001798:Blood Proteins / Q000302:isolation & purification*; D002482:Cellulose / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry*; D006801:Humans
chemical_list: D001798:Blood Proteins; D002482:Cellulose; D005998:Glycine; C100184:tricine