title: Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro.
abstract: While there is evidence of nitric oxide (NO)-dependent signalling via the second messenger cyclic guanosine 3',5'-monophosphate (cGMP) in plants, guanylate cyclases (GCs), enzymes that catalyse the formation of cGMP from guanosine 5'-triphosphate (GTP) have until recently remained elusive and none of the candidates identified to-date are NO-dependent. Using both a GC and heme-binding domain specific (H-NOX) search motif, we have identified an Arabidopsis flavin monooxygenase (At1g62580) and shown electrochemically that it binds NO, has a higher affinity for NO than for O(2) and that this molecule can generate cGMP from GTP in vitro in an NO-dependent manner.
authors.affiliation: Department of Biotechnology, University of the Western Cape, Bellville, South Africa.
authors.forename: Takalani
authors.initials: T
authors.lastname: Mulaudzi
authors.forename: Ndiko
authors.initials: N
authors.lastname: Ludidi
authors.forename: Oziniel
authors.initials: O
authors.lastname: Ruzvidzo
authors.forename: Monique
authors.initials: M
authors.lastname: Morse
authors.forename: Nicolette
authors.initials: N
authors.lastname: Hendricks
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Iwuoha
authors.forename: Chris
authors.initials: C
authors.lastname: Gehring
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004563:Electrochemistry; D006162:Guanylate Cyclase / Q000737:chemistry / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D009569:Nitric Oxide; D006899:Mixed Function Oxygenases; C585561:NOGC1 protein, Arabidopsis; D006162:Guanylate Cyclase; D010100:Oxygen