title: The effects of hypercaloric diets on glucose homeostasis in the rat: influence of saturated and monounsaturated dietary lipids.
abstract: Consumption of energy-dense/high-fat diets is strongly and positively associated with overweight and obesity, which are associated with increase in the prevalence of certain chronic diseases. We evaluated the effect of hypercaloric/fat or normocaloric diets on some biochemical parameters in rats. Seventy-two rats were divided into four groups that were fed for 16 weeks with diets: normocaloric [9.12% soy oil, normocaloric soy oil (NSO)], hypercaloric olive oil [43.8% olive oil, hypercaloric olive oil (HOO)], hypercaloric saturated fat [43.8% saturated fat, hypercaloric saturated fat (HSF)] and normocaloric saturated fat [43.8% saturated fat, normocaloric saturated fat (NSF)]. HSF rats consumed more calories daily than the others and gained more retroperitoneal fat, although HSF and HOO rats had higher body weight. In liver, glycogen synthesis and concentration were higher in rats HSF and NSF. In plasma, total cholesterol (TC) levels were higher in HSF rats than in the others, and triacylglycerol (TAG) levels were lower in HOO and higher in HSF rats in relation to the others. In liver, TC and TAG were elevated in HSF, NSF and HOO rats. Paraoxonase 1 activity, which is related to high-density lipoprotein cholesterol and has anti-atherogenic role was lower in rats HSF. In HOO rats, glucose tolerance test was altered, but insulin tolerance test was normal. These results suggest that consumption of energy-dense/high-fat diets, both saturated or monounsaturated, causes damaging effects. However, more studies are necessary to understand the mechanisms by which these diets cause the metabolic alterations observed.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas e da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: A L
authors.initials: AL
authors.lastname: Hoefel
authors.forename: F
authors.initials: F
authors.lastname: Hansen
authors.forename: P D
authors.initials: PD
authors.lastname: Rosa
authors.forename: A M
authors.initials: AM
authors.lastname: Assis
authors.forename: S L
authors.initials: SL
authors.lastname: Silveira
authors.forename: C C
authors.initials: CC
authors.lastname: Denardin
authors.forename: L
authors.initials: L
authors.lastname: Pettenuzzo
authors.forename: P R
authors.initials: PR
authors.lastname: Augusti
authors.forename: S
authors.initials: S
authors.lastname: Somacal
authors.forename: T
authors.initials: T
authors.lastname: Emanuelli
authors.forename: M L S
authors.initials: ML
authors.lastname: Perry
authors.forename: C M D
authors.initials: CM
authors.lastname: Wannmacher
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D043303:Aryldialkylphosphatase / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005227:Fatty Acids / Q000008:administration & dosage*; D005243:Feces / Q000737:chemistry; D005951:Glucose Tolerance Test; D006003:Glycogen / Q000378:metabolism; D006706:Homeostasis; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000008:administration & dosage; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D015430:Weight Gain
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D005227:Fatty Acids; D000069463:Olive Oil; D010938:Plant Oils; D014280:Triglycerides; D013024:Soybean Oil; D006003:Glycogen; D002784:Cholesterol; D043303:Aryldialkylphosphatase