title: RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
abstract: RNA processing and degradation is initiated by endonucleolytic cleavage of the target RNAs. In many bacteria, this activity is performed by RNase E which is not present in Bacillus subtilis and other Gram-positive bacteria. Recently, the essential endoribonuclease RNase Y has been discovered in B. subtilis. This RNase is involved in the degradation of bulk mRNA suggesting a major role in RNA metabolism. However, only a few targets of RNase Y have been identified so far. In order to assess the global impact of RNase Y, we compared the transcriptomes in response to the expression level of RNase Y. Our results demonstrate that processing by RNase Y results in accumulation of about 550 mRNAs. Some of these targets were substantially stabilized by RNase Y depletion, resulting in half-lives in the range of an hour. Moreover, about 350 mRNAs were less abundant when RNase Y was depleted among them the mRNAs of the operons required for biofilm formation. Interestingly, overexpression of RNase Y was sufficient to induce biofilm formation. The results presented in this work emphasize the importance of RNase Y as the global acting endoribonuclease for B. subtilis.
authors.affiliation: Department of General Microbiology, Georg-August-University Göttingen, Grisebachstr. 8, D-37077 Göttingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Lehnik-Habrink
authors.forename: Marc
authors.initials: M
authors.lastname: Schaffer
authors.forename: Ulrike
authors.initials: U
authors.lastname: Mäder
authors.forename: Christine
authors.initials: C
authors.lastname: Diethmaier
authors.forename: Christina
authors.initials: C
authors.lastname: Herzberg
authors.forename: Jörg
authors.initials: J
authors.lastname: Stülke
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012260:Ribonucleases