title: SOD1 overexpression improves features of the oligodendrocyte precursor response in vitro.
abstract: Spinal cord injury (SCI) produces a significant loss of oligodendrocytes (OL) and demyelination. The oligodendrocyte precursor cells (OPCs) response includes a group of cellular changes in OPCs that are directed to replenish OL loss from the injury. However, this adaptive response is hampered and OPCs eventually die or fail to differentiate to mature and functional OL. In this study, we wanted to evaluate if overexpression of human superoxide dismutase 1 (hSOD1) in OPCs from the SOD1 transgenic rat could improve some of the features of the OPC response in vitro. We found that hSOD1 overexpression increases the proliferation of OPCs and accelerates their differentiation to mature OL in vitro. Furthermore, hSOD1 overexpression reduces oxidative stress-mediated death in OPCs. These results suggest hSOD1 as a therapeutic target to increase OPC response success and potentially, OL replacement and remyelination after SCI.
authors.affiliation: Brain and Spinal Injury Center, Department of Neurological Surgery, University of California, San Francisco, United States.
authors.forename: S
authors.initials: S
authors.lastname: Veiga
authors.forename: J
authors.initials: J
authors.lastname: Ly
authors.forename: P H
authors.initials: PH
authors.lastname: Chan
authors.forename: J C
authors.initials: JC
authors.lastname: Bresnahan
authors.forename: M S
authors.initials: MS
authors.lastname: Beattie
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D005732:Gangliosides / Q000378:metabolism; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000378:metabolism; D009836:Oligodendroglia / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055647:Rats, Transgenic; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000072105:Superoxide Dismutase-1; D013997:Time Factors; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
chemical_list: D039481:CD11b Antigen; D005732:Gangliosides; D036341:Intercellular Signaling Peptides and Proteins; D004676:Myelin Basic Protein; C000606290:SOD1 protein, human; C062875:ganglioside A2B5; D020122:tert-Butylhydroperoxide; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D001973:Bromodeoxyuridine