title: [Influences of Oxytropis falcata on proliferation of SMMC-7721 hepatocarcinoma cells and expression of MMP-2].
abstract: OBJECTIVE
  To investigate the influences of extracts of Oxytropis falcata on proliferation of SMMC-7721 cells and expression of MMP-2 (matrix metalloproteinase-2).
  
  
  METHODS
  SMMC-7721 cells were treated for 24 h with five fractions obtained from 0. falcata in different concentrations. Inhibition on proliferation of the SMMC-7721 cells was assessed by MT method. Secretion of MMP-2 was measured by ELISA in the supernatant of SMMC-7721 cells treated with fractions of essential oil and total flavonoids for 24 h. Transcription of mRNA of MMP-2 was detected by Real-time PCR.
  
  
  RESULTS
  In MT assay, essential oil and total flavonoids showed potential antiproliferative activity on SMMC-7721 in a concentration dependent manner. Data of ELISA showed that fraction of essential oil suppressed secretion of MMP-2 significantly. Results of Real-time PCR indicated that both essential oil and total flavonoids restrained expression of mRNA of MMP-2.
  
  
  CONCLUSIONS
  It suggested that essential oil and total flavonoids of O. falcata inhibit proliferation of SMMC-7721 cells through down-regulating secretion and expression of MMP-2 in cells. However, further experiments are necessary to carry out to investigate the potential mechanism.
authors.affiliation: Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Key Laboratory of State Administration of Traditional Chinese Medicine for Standardization of Chinese Medicine Processing, Nanjing 210029, China. ygmm0901@hotmail.com
authors.forename: Guangming
authors.initials: G
authors.lastname: Yang
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: He
authors.forename: Li
authors.initials: L
authors.lastname: Tong
authors.forename: Baochangi
authors.initials: B
authors.lastname: Cai
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D033021:Oxytropis / Q000737:chemistry*; D016133:Polymerase Chain Reaction
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D020778:Matrix Metalloproteinase 2