title: A novel immune-related gene, microtubule aggregate protein homologue, is up-regulated during IFN-γ-related immune responses in Japanese flounder, Paralichthys olivaceus.
abstract: Delayed-type hypersensitivity (DTH) response mediated by antigen-specific Th1 cells is used as a test to detect exposure to tuberculosis in humans. Japanese flounder (Paralichthys olivaceus) microtubule aggregate protein homologue (PoMTAP) was identified as a gene strongly induced during fish DTH response. In this study, PoMTAP gene was cloned and its expression profile was analyzed. The PoMTAP gene has a transcriptional regulatory region that includes two interferon-stimulated response elements and two IFN-γ activated sites. Expressions of PoMTAP and IFN-γ genes were up-regulated at the same time points during the DTH response, Edwardsiella tarda infection and VHSV infection. Furthermore, PoMTAP gene expressing cells also expressed CD3ε, confirming that PoMTAP is expressed by T lymphocytes. These results suggest that PoMTAP is a novel immune-related gene expressed by T lymphocytes that is preferentially induced by IFN-γ and has a role in Th1-mediated immune responses in Japanese flounder.
authors.affiliation: Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Tokyo, Japan.
authors.forename: Goshi
authors.initials: G
authors.lastname: Kato
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005432:Flounder / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000235:genetics*; D017403:In Situ Hybridization / Q000662:veterinary; D007371:Interferon-gamma / Q000276:immunology*; D008868:Microtubule Proteins / Q000235:genetics / Q000276:immunology*; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015854:Up-Regulation
chemical_list: D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D012313:RNA; D007371:Interferon-gamma