title: Antibacterial activity and cytocompatibility of chitosan-N-hydroxy-2,3-propyl-N methyl-N,N-diallylammonium methyl sulfate.
abstract: A water-soluble quaternary ammonium salt of chitosan, chitosan-N-hydroxy-2,3-propyl-N-methyl-N,N-diallylammonium methyl sulfate (MDAACS), was synthesized by reacting chitosan with methyl diallyl ammonium salt (MDAA). The results of water contact angle and swelling ratio showed that the membrane of MDAACS was more hydrophilic than chitosan. The antibacterial activities of MDAACS against Staphylococcus aureus and Klebsiella pneumoniae were evaluated with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The results showed that the antibacterial activity of MDAACS was higher than that of chitosan. The cytocompatibility was evaluated in vitro with L929 fibroblast proliferation based on MTT colorimetric assay. The results showed that cell growth was much higher on MDAACS than on chitosan.
authors.affiliation: Department of Materials and Textile, Oriental Institute of Technology, Pan-Chiao District, New Taipei City, Taiwan. fc015@mail.oit.edu.tw
authors.forename: Chi-Hsiung
authors.initials: CH
authors.lastname: Jou
mesh_terms: D000900:Anti-Bacterial Agents / Q000009:adverse effects / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007711:Klebsiella pneumoniae / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000644:Quaternary Ammonium Compounds; D048271:Chitosan