title: Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption.
abstract: Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required for efficient microbial DNA extraction from intestinal biopsies.
authors.affiliation: Department of Animal Sciences, University of Illinois at Urbana-Champaign, IL 61801, USA.
authors.forename: Franck
authors.initials: F
authors.lastname: Carbonero
authors.forename: Gerardo M
authors.initials: GM
authors.lastname: Nava
authors.forename: Ann C
authors.initials: AC
authors.lastname: Benefiel
authors.forename: Eugene
authors.initials: E
authors.lastname: Greenberg
authors.forename: H Rex
authors.initials: HR
authors.lastname: Gaskins
mesh_terms: D000704:Analysis of Variance; D001426:Bacterial Proteins / Q000235:genetics; D001706:Biopsy; D002458:Cell Fractionation / Q000379:methods*; D003106:Colon / Q000382:microbiology; D059018:DNA Contamination; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006801:Humans; D007082:Ileum / Q000382:microbiology; D058889:Molecular Typing / Q000379:methods; D011933:Reagent Kits, Diagnostic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic