title: Impact of toll-like receptor 2 deficiency on immune responses to mycobacterial antigens.
abstract: In the present study, we addressed the question of whether Toll-like receptor 2 (TLR2)-mediated innate immunity can contribute to the development of acquired immune responses. We immunized TLR2(-/-) and wild-type (WT) mice three times subcutaneously with the mycobacterial antigen (Ag19kDa) (a TLR2 ligand) or Ag85A (not a TLR2 ligand). One week after the last immunization, sera and spleens were collected. To evaluate cellular responses, we measured gamma interferon (IFN-γ) after in vitro restimulation of spleen cells with antigen alone or antigen-pulsed bone marrow-derived macrophages (BMM(Ag)) or pulmonary macrophages (PuM(Ag)). Antibody responses were comparable in the two mouse strains, but we observed differences in the cellular responses. Recall responses to Ag85A were similar in the two strains, but responses to Ag19kDa given alone or presented by BMM or PuM were lower in TLR2(-/-) than in WT mice. The largest differences in cellular responses were observed when Ag19kDa was presented by PuM. To understand this, we analyzed phenotypic and functional differences between BMM and PuM upon stimulation with various ligands. Generally, PuM had a lower response to the TLR2 ligand Pam(3)Cys-Ser-(Lys)(4) trihydrochloride and to anti-CD40 than BMM, as measured by cytokine secretion and upregulation of costimulatory molecules. This might provide a partial explanation for the lower capacity of PuM when pulsed with Ag19kDa, also a TLR2 ligand. Altogether, our results revealed weaknesses in the T cell and antigen-presenting cell (APC) compartments of the Ag19kDa-immunized TLR2(-/-) mice but indicated that specific immune responses could be generated in the absence of TLR2 regardless of the characteristics of the antigen used.
authors.affiliation: Department of Immunology, Wenner-Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden.
authors.forename: Muhammad J
authors.initials: MJ
authors.lastname: Rahman
authors.forename: Olga D
authors.initials: OD
authors.lastname: Chuquimia
authors.forename: Dagbjort H
authors.initials: DH
authors.lastname: Petursdottir
authors.forename: Natalia
authors.initials: N
authors.lastname: Periolo
authors.forename: Mahavir
authors.initials: M
authors.lastname: Singh
authors.forename: Carmen
authors.initials: C
authors.lastname: Fernández
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009161:Mycobacterium / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D051195:Toll-Like Receptor 2 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; C493486:Tlr2 protein, mouse; D051195:Toll-Like Receptor 2; D007371:Interferon-gamma