title: Nanocomposites containing silica-coated gold-silver nanocages and Yb-2,4-dimethoxyhematoporphyrin: multifunctional capability of IR-luminescence detection, photosensitization, and photothermolysis.
abstract: We describe novel composite nanoparticles consisting of a gold-silver nanocage core and a mesoporous silica shell functionalized with the photodynamic sensitizer Yb-2,4-dimethoxyhematoporphyrin (Yb-HP). In addition to the long-wavelength plasmon resonance near 750-800 nm, the composite particles exhibited a 400-nm absorbance peak and two fluorescence peaks, near 580 and 630 nm, corresponding to bound Yb-HP. The fabricated nanocomposites generated singlet oxygen under 630-nm excitation and produced heat under laser irradiation at the plasmon resonance wavelength (750-800 nm). In particular, we observed enhanced killing of HeLa cells incubated with nanocomposites and irradiated by 630-nm light. Furthermore, an additional advantage of fabricated conjugates was an IR-luminescence band (900-1060 nm), originating from Yb(3+) ions of bound Yb-HP and located in the long-wavelength part of the tissue transparency window. This modality was used to control the accumulation and biodistribution of composite particles in mice bearing Ehrlich carcinoma tumors in a comparative study with intravenously injected free Yb-HP molecules. Thus, these multifunctional nanocomposites seem an attractive theranostic platform for simultaneous IR-luminescence diagnostic and photodynamic therapy owing to Yb-HP and for plasmonic photothermal therapy owing to Au-Ag nanocages.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
authors.forename: Boris
authors.initials: B
authors.lastname: Khlebtsov
authors.forename: Elizaveta
authors.initials: E
authors.lastname: Panfilova
authors.forename: Vitaly
authors.initials: V
authors.lastname: Khanadeev
authors.forename: Olga
authors.initials: O
authors.lastname: Bibikova
authors.forename: Georgy
authors.initials: G
authors.lastname: Terentyuk
authors.forename: Andrey
authors.initials: A
authors.lastname: Ivanov
authors.forename: Valentina
authors.initials: V
authors.lastname: Rumyantseva
authors.forename: Igor
authors.initials: I
authors.lastname: Shilov
authors.forename: Anastasia
authors.initials: A
authors.lastname: Ryabova
authors.forename: Victor
authors.initials: V
authors.lastname: Loshchenov
authors.forename: Nikolai G
authors.initials: NG
authors.lastname: Khlebtsov
mesh_terms: D006046:Gold / Q000737:chemistry*; D006415:Hematoporphyrins / Q000737:chemistry*; D049449:Luminescence; D053761:Nanocomposites; D010777:Photochemistry; D012834:Silver / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods*; D015018:Ytterbium / Q000737:chemistry*
chemical_list: D006415:Hematoporphyrins; D012834:Silver; D006046:Gold; D015018:Ytterbium