title: Identification of immuno-reactive adult Angiostrongylus vasorum proteins using mass spectrometry.
abstract: Angiostrongylus vasorum is an emerging parasite of dogs and related carnivores throughout western Europe and presents a biosecurity threat to many countries worldwide. Infections are difficult to diagnose due to a high variability of clinical signs and can be fatal if left untreated. Previous attempts to develop indirect ELISA as a diagnostic test for antibody presence in dogs have been limited by cross-reactive recognition of antibodies to other endemic nematodes. This study compared the immuno-dominant soluble somatic proteins for first stage, third stage and adult A. vasorum using Western blotting. 2D electrophoresis and mass spectrometry were further used to identify specific immuno-dominant proteins (n=14) within the soluble fraction from whole adult worms. Proteins included an aspartyl protease inhibitor, representing a potential candidate for a recombinant-based ELISA and novel vaccine.
authors.affiliation: School of Clinical Veterinary Sciences, University of Bristol, Langford, UK.
authors.forename: Ryan
authors.initials: R
authors.lastname: Jefferies
authors.forename: Eric R
authors.initials: ER
authors.lastname: Morgan
authors.forename: Susan
authors.initials: S
authors.lastname: Shaw
authors.forename: Kate
authors.initials: K
authors.lastname: Heesom
mesh_terms: D000801:Angiostrongylus / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D015153:Blotting, Western; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D017206:Strongylida Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins