title: Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities.
abstract: Crude drugs expected to have an estrogenic effect were screened for their inhibitory activity on testosterone 5α-reductase. Testosterone 5α-reductase is an enzyme catalyzing the conversion of testosterone to dihydrotestosterone, which possesses high affinity for the androgen receptor. Among the crude drugs tested, we focused on Puerariae Flos (the flowers of Pueraria thomsonii) due to its potent inhibitory activity and suitability for commercial use. The 50% ethanolic extract of Puerariae Flos (PF-ext) showed inhibitory activity of 60.2% at 500 μg/ml against testosterone 5α-reductase. Interestingly, it was more potent than that of Puerariae Radix (roots of Pueraria lobata). PF-ext also showed in vivo anti-androgenic activity using a hair growth assay in testosterone-sensitive male C57Black/6NCrSlc strain mice. We demonstrated saponins, including soyasaponin I and kaikasaponin III, to be active components in PF-ext. In addition, hair growth promotion activity in C3H/He mice at 2 mg/mouse/day of the topical administration of PF-ext was demonstrated. Thus, Puerariae Flos is a promising crude drug for treating androgenic alopecia.
authors.affiliation: Faculty of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Murata
authors.forename: Kazuma
authors.initials: K
authors.lastname: Noguchi
authors.forename: Masato
authors.initials: M
authors.lastname: Kondo
authors.forename: Mariko
authors.initials: M
authors.lastname: Onishi
authors.forename: Naoko
authors.initials: N
authors.lastname: Watanabe
authors.forename: Katsumasa
authors.initials: K
authors.lastname: Okamura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D058891:5-alpha Reductase Inhibitors / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000287:Administration, Topical; D000505:Alopecia / Q000188:drug therapy* / Q000201:enzymology / Q000503:physiopathology; D000726:Androgen Antagonists / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D035264:Flowers; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018517:Plant Roots; D010946:Plants, Medicinal; D029908:Pueraria / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D058891:5-alpha Reductase Inhibitors; D000726:Androgen Antagonists; D004365:Drugs, Chinese Herbal; D012997:Solvents; D000431:Ethanol; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase