title: Lactic acid bacteria isolated from ethnic preserved meat products of the Western Himalayas.
abstract: We used culture- and molecular-biology-based methods to investigate the diversity of lactic acid bacteria (LAB) in the ethnic chevon (goat) meat products chartayshya, jamma and arjia of the Western Himalayas. In six chartayshya, six jamma and four arjia samples, LAB were the predominant microbial component involved in the fermentation of these samples, and the total LAB population in arjia (7.8 ± 0.1 log cfu g(-1); mean ± SD) was significantly higher (P < 0.05) than in chartayshya (6.9 ± 0.1 log cfu g(-1)) and jamma (7.5 ± 0.1 log cfu g(-1)). We identified 53 LAB samples by 16S rRNA and phenylalanyl-tRNA synthase (pheS) genes sequencing. The LAB isolates were identified as Enterococcus durans, Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Leuconostoc citreum, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria. These results revealed that there is a high level of diversity of LAB in the Himalayan ethnic preserved meat products.
authors.affiliation: Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan.
authors.forename: Kaihei
authors.initials: K
authors.lastname: Oki
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Rai
authors.forename: Sumie
authors.initials: S
authors.lastname: Sato
authors.forename: Koichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Jyoti Prakash
authors.initials: JP
authors.lastname: Tamang
mesh_terms: D016983:Enterococcus / Q000302:isolation & purification; D056546:Lactobacillales / Q000145:classification / Q000302:isolation & purification*; D007934:Leuconostoc / Q000302:isolation & purification; D008461:Meat Products / Q000382:microbiology*; D009390:Nepal; D010376:Pediococcus / Q000302:isolation & purification; D010802:Phylogeny; D058836:Weissella / Q000302:isolation & purification