title: Proteomics to study the diversity and dynamics of proteasome complexes: from fundamentals to the clinic.
abstract: This article covers the latest contributions of proteomics to the structural and functional characterization of proteasomes and their associated proteins, but also to the detection of proteasomes as clinical biomarkers in diseases. Proteasomes are highly heterogenous supramolecular complexes and constitute important cellular proteases controlling the pool of proteins involved in key cellular functions. The comprehension of the structure/function relationship of proteasomes is therefore of major interest in biology. Numerous biochemical methods have been employed to purify proteasomes, and have led to the identification of complexes of various compositions - depending on the experimental conditions and the type of strategy used. In association with protein separation and enrichment techniques, modern mass spectrometry instruments and mass spectrometry-based quantitative methods, they have led to unprecedented breakthroughs in the in-depth analysis of the diversity and dynamics of proteasome composition and localization under various stimuli or pathological contexts. Proteasome inhibitors are now used in clinics for the treatment of cancer, and recent studies propose that the proteasome should be considered as a predictive biomarker for various pathologies.
authors.affiliation: CNRS, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Bousquet-Dubouch
authors.forename: Bertrand
authors.initials: B
authors.lastname: Fabre
authors.forename: Bernard
authors.initials: B
authors.lastname: Monsarrat
authors.forename: Odile
authors.initials: O
authors.lastname: Burlet-Schiltz
mesh_terms: D006801:Humans; D013058:Mass Spectrometry; D046988:Proteasome Endopeptidase Complex / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D057170:Translational Research, Biomedical
chemical_list: D046988:Proteasome Endopeptidase Complex