title: Circadian Clock genes Per2 and clock regulate steroid production, cell proliferation, and luteinizing hormone receptor transcription in ovarian granulosa cells.
abstract: Circadian Clock genes are associated with the estrous cycle in female animals. Treatment with Per2 and Clock siRNAs decreased the number of granulosa cells and LHr expression in follicle-stimulating hormone FSH-treated granulosa cells. Per2 siRNA treatment did not stimulate the production of estradiol and expression of P450arom, whereas Clock siRNA treatment inhibited the production of estradiol and expression of P450arom mRNA. Per2 and Clock siRNA treatment increased and unchanged, respectively, progesterone production in FSH-treated granulosa cells. Similarly, expression of StAR mRNA was increased by Per2 siRNA and unchanged by Clock siRNA. Our data provide a new insight that Per2 and Clock have different action on ovarian granulosa cell functions.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. shimizut@obihiro.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hirai
authors.forename: Chiaki
authors.initials: C
authors.lastname: Murayama
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Koyomi
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D001141:Aromatase / Q000235:genetics; D056926:CLOCK Proteins / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation; D057906:Circadian Clocks / Q000235:genetics*; D004958:Estradiol / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D011974:Receptors, LH / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D056950:Period Circadian Proteins; D034741:RNA, Small Interfering; D011974:Receptors, LH; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D056926:CLOCK Proteins