title: [Determination of ergosterol in Ganoderma lucidum from different varieties and cultured tree species by HPLC].
abstract: OBJECTIVE
  HPLC method for determining ergosterol was established to evaluate the quality of Ganoderma lucidum and to analyze of the ergosterol in different varieties and cultrued tree species.
  
  
  METHODS
  Samples from 17 different varieties and 14 different tree species were quantified by HPLC. The RP-HPLC was conducted on Diamonsil C18 column with acetonitrile as the mobile phase at 40 degrees C. The flow rate was 1.0 mL/min; and the detection wavelength was 282 nm.
  
  
  RESULTS
  The fluctuations of ergosterol content was 0.093%-0.243% among different varieties and 0.080%-0.227% among different tree species. Hanzhi-2, Yuanzhi, Ming-1, Yesheng-1 hao were excellent at both ergosterol content and conversion rate among all the varieties, and Bai Li, Yang Mei have comparatively more ergosterol and high conversion rate among all the tree species in this study.
  
  
  CONCLUSIONS
  This method is simple, sensitive and accurate. It could be used to determine the contents of ergosterol and the evaluation of the quality in Ganoderma. It also provides references for choosing varieties and tree species when culture Ganoderma.
authors.affiliation: Chinese Academy of Medical Sciences/Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100193, China. jing_jing_6@163.com
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Wen-hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: Lv
authors.forename: Jin-ping
authors.initials: JP
authors.lastname: Si
authors.forename: Bao-lin
authors.initials: BL
authors.lastname: Guo
authors.forename: Shi-jie
authors.initials: SJ
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004875:Ergosterol / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D020940:Reishi / Q000737:chemistry* / Q000145:classification / Q000254:growth & development*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D014197:Trees / Q000145:classification
chemical_list: D012997:Solvents; D004875:Ergosterol