title: Molecular identification of a causative parasite species using formalin-fixed paraffin embedded (FFPE) tissues of a complicated human pulmonary sparganosis case without decisive clinical diagnosis.
abstract: PCR-based molecular diagnosis was made for the identification of causative agents of the clinically suspected pulmonary proliferative sparganosis case found in Thailand using formalin-fixed paraffin-embedded (FFPE) biopsy specimens. As a reference, FFPE biopsy specimen from a typical cutaneous sparganosis case was examined together. DNA samples were extracted from tissues and two partial fragments of cytochrome c oxidase subunit 1 (cox1) gene were amplified for the detection of Spirometra DNA. Two cox1 fragments were amplified successfully for both specimens. After alignment of nucleotide sequences of the PCR-amplicons, the causative agents of both cases were identified as Spirometra erinaceieuropaei.
authors.affiliation: Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Supinda
authors.initials: S
authors.lastname: Koonmee
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yamasaki
authors.forename: Hiromu
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Maki
authors.initials: M
authors.lastname: Muto
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kuramochi
authors.forename: Jurairat
authors.initials: J
authors.lastname: Kularbkeaw
authors.forename: Jaturat
authors.initials: J
authors.lastname: Kanpittaya
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
authors.forename: Yukifumi
authors.initials: Y
authors.lastname: Nawa
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D001706:Biopsy; D018509:DNA, Helminth / Q000032:analysis*; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics*; D017809:Fatal Outcome; D006801:Humans; D008168:Lung / Q000469:parasitology* / Q000503:physiopathology; D008297:Male; D008867:Microtomy; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013031:Sparganosis / Q000175:diagnosis* / Q000469:parasitology / Q000503:physiopathology; D013032:Sparganum / Q000254:growth & development / Q000648:ultrastructure*; D013785:Thailand; D016707:Tissue Fixation
chemical_list: D018509:DNA, Helminth; D021122:Protein Subunits; D003576:Electron Transport Complex IV