title: Photo-cured hyaluronic acid-based hydrogels containing simvastatin as a bone tissue regeneration scaffold.
abstract: We describe in this study the positive influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with simvastatin (SIM). Prior to loading SIM, we first characterized the HA hydrogels for their mechanical properties and swelling ratios. The results from this testing indicated that these two factors improved as the substitution degree of 2-aminoethyl methacrylate (AEMA) increased. MTT and live/dead assays showed that the HA hydrogels have good biocompatibility for use as scaffolds for bone tissue regeneration. Moreover, another MTT assay showed that the photo-cured HA hydrogels III fabricated with 30% AEMA (300 mg) conjugated HA (HA-AEMA iii) loaded with between 0.1 and 1 mg of SIM had a similar cytotoxicity as compared to the HA hydrogel III itself. The sustained release of SIM was observed to occur in the HA hydrogel III loaded with 1 mg of SIM. In vitro and in vivo experiments showed that the HA hydrogel III loaded with 1 mg of SIM had a significant influence on osteogenesis.
authors.affiliation: Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Bae
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.forename: Yong-Dae
authors.initials: YD
authors.lastname: Kwon
authors.forename: In Chan
authors.initials: IC
authors.lastname: Youn
authors.forename: Kuiwon
authors.initials: K
authors.lastname: Choi
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Hong
authors.forename: Gye Tae
authors.initials: GT
authors.lastname: Kim
authors.forename: Yong Suk
authors.initials: YS
authors.lastname: Choi
authors.forename: Eui Hwan
authors.initials: EH
authors.lastname: Hwang
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects / Q000528:radiation effects; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D058105:Polymerization / Q000187:drug effects / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012212:Rheology / Q000187:drug effects / Q000528:radiation effects; D019821:Simvastatin / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties / Q000187:drug effects / Q000528:radiation effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D008689:Methacrylates; D012333:RNA, Messenger; C072703:2-aminoethylmethacrylate; D006820:Hyaluronic Acid; D019821:Simvastatin