title: Expression, purification and preliminary crystallographic analysis of O-acetylhomoserine sulfhydrylase from Mycobacterium tuberculosis.
abstract: The gene product of the open reading frame Rv3340 from Mycobacterium tuberculosis is annotated as encoding a probable O-acetylhomoserine (OAH) sulfhydrylase (MetC), an enzyme that catalyzes the last step in the biosynthesis of methionine, which is an essential amino acid in bacteria and plants. Following overexpression in Escherichia coli, the M. tuberculosis MetC enzyme was purified and crystallized using the hanging-drop vapor-diffusion method. Native diffraction data were collected from crystals belonging to space group P2(1) and were processed to a resolution of 2.1 Å.
authors.affiliation: Department of Biochemistry, School of Molecular and Systems Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yin
authors.forename: Craig R
authors.initials: CR
authors.lastname: Garen
authors.forename: Katherine
authors.initials: K
authors.lastname: Bateman
authors.forename: Minmin
authors.initials: M
authors.lastname: Yu
authors.forename: Emily Z Alipio
authors.initials: EZ
authors.lastname: Lyon
authors.forename: Jeff
authors.initials: J
authors.lastname: Habel
authors.forename: Heungbok
authors.initials: H
authors.lastname: Kim
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Hung
authors.forename: Chang-Yub
authors.initials: CY
authors.lastname: Kim
authors.forename: Michael N G
authors.initials: MN
authors.lastname: James
mesh_terms: D000595:Amino Acid Sequence; D019757:Carbon-Oxygen Lyases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology*; D016415:Sequence Alignment
chemical_list: C018725:O-acetylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases