title: Flow-injection chemiluminescence determination of melamine in urine and plasma.
abstract: A novel flow-injection chemiluminescence method for the determination of melamine in urine and plasma was developed. It was found that melamine can remarkably enhance chemiluminescence emission from the luminol-K(3) Fe(CN)(6) system in an alkaline medium. Under the optimum conditions, chemiluminescence intensity had a good linear relationship with the concentration of melamine in the range 9.0 × 10(-9) -7.0 × 10(-6) g/mL, with a correlation coefficient of 0.9992. The detection limit (3σ) was 3.5 ng/mL. The method has been applied to determine the concentration of melamine in samples using liquid-liquid extraction. Average recoveries of melamine were 102.6% in urine samples and 95.1% in plasma samples. The method provided a reproducible and stable approach for the sensitive detection of melamine in urine and plasma samples.
authors.affiliation: Second Affiliated Hospital, Lanzhou University, Lanzhou 730000, People's Republic of China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Tang
authors.forename: Xiyan
authors.initials: X
authors.lastname: Shi
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhongjin
authors.initials: Z
authors.lastname: Yue
authors.forename: Qiqi
authors.initials: Q
authors.lastname: He
mesh_terms: D000818:Animals; D002648:Child; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007700:Kinetics; D059627:Liquid Phase Microextraction; D049449:Luminescence; D012756:Sheep; D014227:Triazines / Q000097:blood* / Q000652:urine*
chemical_list: D014227:Triazines; C011907:melamine