title: Serum from interferon-β-1b-treated patients with early multiple sclerosis stabilizes the blood-brain barrier in vitro.
abstract: Interferon-β (IFN-β) stabilizes the blood-brain barrier (BBB) in vitro. Here we investigated the effect of serum from 15 IFN-β-1b-treated multiple sclerosis (MS) patients on the permeability read-outs of small solutes in an in vitro BBB model consisting of human brain microvascular endothelial cells in co-culture with rat astrocytes. The addition of sera from IFN-β-treated patients resulted in a significantly (p < 0.05) reduced permeability as compared with untreated patients. Our findings show that sera from IFN-β-1b-treated MS patients have a stabilizing effect on the in vitro BBB. We suggest an unknown potentially pro-inflammatory factor in the serum of MS patients that may lead to a BBB dysfunction and can be modulated by IFN-β.
authors.affiliation: Department of Neurology, University Hospital of Münster, Münster, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Müller
authors.forename: Achim
authors.initials: A
authors.lastname: Frese
authors.forename: Isabelle
authors.initials: I
authors.lastname: Nassenstein
authors.forename: Maike
authors.initials: M
authors.lastname: Hoppen
authors.forename: Martin
authors.initials: M
authors.lastname: Marziniak
authors.forename: Erich Bernd
authors.initials: EB
authors.lastname: Ringelstein
authors.forename: Kwang Sik
authors.initials: KS
authors.lastname: Kim
authors.forename: Wolf-Rüdiger
authors.initials: WR
authors.lastname: Schäbitz
authors.forename: Jörg
authors.initials: J
authors.lastname: Kraus
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000328:Adult; D000818:Animals; D001253:Astrocytes / Q000166:cytology; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000494:pharmacology*; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002461:Cell Line, Transformed; D018920:Coculture Techniques; D016903:Drug Monitoring / Q000379:methods; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D051381:Rats
chemical_list: D000276:Adjuvants, Immunologic; D015415:Biomarkers; D001798:Blood Proteins; D000068576:Interferon beta-1b; D016899:Interferon-beta