title: Catalytic and thermodynamic properties of a tannase produced by Aspergillus niger GH1 grown on polyurethane foam.
abstract: Tannase is an inducible enzyme with important applications in the food and pharmaceutical industries. This enzyme was produced by the fungus Aspergillus niger GH1 under solid-state fermentation using polyurethane foam as solid support and tannic acid as sole carbon source and tannase inducer. Physicochemical properties of A. niger tannase were characterized, and the kinetic and thermodynamics parameters on methyl gallate hydrolysis were evaluated. The enzyme was stable in a pH range of 2-8 and a functional temperature range of 25-65 °C. The highest k(cat) value was 2,611.10 s(-1) at 65 °C. Tannase had more affinity for methyl gallate at 45 °C with a K(M) value of 1.82 mM and an efficiency of hydrolysis (k(cat)/K(M)) of 330.01 s(-1) mM(-1). The lowest E(a) value was found to be 21.38 kJ/mol at 4.4 mM of methyl gallate. The lowest free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 64.86 and 18.56 kJ/mol, respectively. Entropy (ΔS) was -0.22 kJ/mol K. Results suggest that the A. niger GH1 tannase is an attractive enzyme for industrial applications due its catalytic and thermodynamical properties.
authors.affiliation: Department of Food Science and Technology, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, Coahuila, Mexico.
authors.forename: Erika L
authors.initials: EL
authors.lastname: Ramos
authors.forename: Marco A
authors.initials: MA
authors.lastname: Mata-Gómez
authors.forename: Luis V
authors.initials: LV
authors.lastname: Rodríguez-Durán
authors.forename: Ruth E
authors.initials: RE
authors.lastname: Belmares
authors.forename: Raúl
authors.initials: R
authors.lastname: Rodríguez-Herrera
authors.forename: Cristóbal Noe
authors.initials: CN
authors.lastname: Aguilar
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development; D061249:Batch Cell Culture Techniques / Q000295:instrumentation; D055162:Biocatalysis; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011140:Polyurethanes / Q000032:analysis
chemical_list: D005656:Fungal Proteins; D011140:Polyurethanes; C028279:polyurethane foam; D002265:Carboxylic Ester Hydrolases; C022711:tannase