title: The antifouling potentiality of galactosamine characterized from Vibrio vulnificus exopolysaccharide.
abstract: To gain a better insight into biofilm composition, the exopolysaccharide (EPS) of the Gram-negative bacterium Vibrio vulnificus was studied. Monosaccharide composition analysis of the wild-type and mutant V. vulnificus EPS carried out with Bio-liquid chromatography revealed the presence of D-glucosamine, D-galactose, D-glucose and D-xylose in both strains. D-galactosamine was found only in the mutant that formed less biofilm compared to its wild-type. The influence of galactosamine on biofilm formation was then studied by adding this substance gradually to six different Gram-negative/positive bacteria associated with various autoinducers. Four bacterial species known to use the autoinducer type-2 signaling system produced less biofilm in the presence of galactosamine. No significant inhibition of biofilm formation was observed in bacteria that produce autoinducer type-1 signal molecules. Galactosamine was also immobilized on polymeric nanofibers to determine its re-usability for the study of biofilm inhibition. The immobilized galactosamine retained >65% of its initial antifouling activity after 10 repeated uses. The results of this study suggest the antifouling role of galactosamine for bacteria that produce AI-2.
authors.affiliation: Department of Microbiology, Chungbuk National University, 410 Sungbong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Kim
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Um
authors.forename: Kyu-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Hongik
authors.initials: H
authors.lastname: Kim
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002853:Chromatography, Liquid; D005688:Galactosamine / Q000494:pharmacology*; D005944:Glucosamine / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D009005:Monosaccharides / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D015398:Signal Transduction; D041261:Vibrio vulnificus / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D005688:Galactosamine; D005944:Glucosamine