title: Virus load in pigs affected with different clinical forms of classical swine fever.
abstract: Classical swine fever (CSF) is an endemic disease in India, but the real magnitude of the problem is not known as only outbreaks of acute CSF are reported and many cases of chronic and clinically inapparent forms of the disease, which manifest a confusing clinical picture, remain undiagnosed. The real status of classical swine fever virus (CSFV) infection can only be known by testing pigs with highly specific and sensitive diagnostic assays. To obtain the baseline prevalence of CSFV infection among pigs in an endemic region where no vaccination was being performed, a real-time PCR assay was used to detect viral genetic material in tissue samples collected from a slaughterhouse in the northern state of Uttar Pradesh in India. In total, 1120 slaughtered pigs were examined for the presence of CSF suggestive pathological lesions and tissues from suspected cases were tested for the presence of CSFV antigen and nucleic acids by indirect immuno-peroxidase test and real-time PCR, respectively. Based on the detection of viral genetic material in the tonsils, the prevalence of CSFV infection among slaughtered pigs was found to be 7.67%. Pigs detected positive for viral genome by quantitative real-time PCR assay when categorized into different forms of CSF, depending upon the pathological lesions observed, the viral load in the tonsils of some of the pigs with chronic or clinically inapparent form of the disease was similar to that detected in pigs with acute CSF. The results of the study suggested that the risk posed by pigs with chronic disease or those infected but showing no clinical disease may be relatively higher as they can transmit the virus to new susceptible hosts over a longer period of time.
authors.affiliation: National CSF Referral Laboratory, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
authors.forename: M
authors.initials: M
authors.lastname: Rout
authors.forename: G
authors.initials: G
authors.lastname: Saikumar
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000302:isolation & purification*; D007194:India / Q000453:epidemiology; D014066:Palatine Tonsil / Q000821:virology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D019562:Viral Load