title: Simple and accurate quantitative analysis of ten antiepileptic drugs in human plasma by liquid chromatography/tandem mass spectrometry.
abstract: A simple, accurate, and sensitive liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method has been developed for the simultaneous quantification of 10 antiepileptic drugs (AEDs; gabapentin (GBP), levetiracetam (LEV), valproic acid (VPA), lamotrigine (LTG), carbamazepine-10,11-epoxide (CBZ-epoxide), zonisamide (ZNS), oxcarbazepine (OXC), topiramate (TPM), carbamazepine (CBZ), phenytoin (PHT)) in human plasma as a tool for drug monitoring. d(10)-Phenytoin (d(10)-PHT) and d(6-)valproic acid (d(6)-VPA) were used as internal standards for the positive- and negative-ionization modes, respectively. Plasma samples were precipitated by the addition of acetonitrile, and supernatants were analyzed on a C18 reverse-phase column using an isocratic elution. Detection was carried out in selected reaction monitoring (SRM) mode. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r(2)) greater than 0.997 for all AEDs. The intra- and inter-day precision was less than 12%, and the accuracy was between 85.9 and 114.5%. This method was successfully used in the identification and quantitation of AEDs in patients undergoing mono- or polytherapy for epilepsy.
authors.affiliation: Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan, South Korea.
authors.forename: Kwon-Bok
authors.initials: KB
authors.lastname: Kim
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Seo
authors.forename: Sung-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Bae
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Jae-Gook
authors.initials: JG
authors.lastname: Shin
mesh_terms: D000927:Anticonvulsants / Q000097:blood* / Q000737:chemistry / Q000627:therapeutic use; D002138:Calibration; D002220:Carbamazepine / Q000097:blood* / Q000737:chemistry / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring; D004355:Drug Stability; D004827:Epilepsy / Q000188:drug therapy; D006801:Humans; D016014:Linear Models; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014635:Valproic Acid / Q000097:blood* / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000927:Anticonvulsants; D002220:Carbamazepine; D014635:Valproic Acid