title: The influence of silicon substitution on the properties of spherical- and whisker-like biphasic α-calcium-phosphate/hydroxyapatite particles.
abstract: In this work, the influence of the morphology of hydroxyapatite particles on silicon substitution through hydrothermal synthesis performed under the same conditions was investigated. Spherical- and whisker-like hydroxyapatite particles were obtained starting from calcium-nitrate, sodium dihydrogen phosphate, disodium-ethylenediaminetetraacetic acid and urea (used only for the synthesis of whisker-like particles) dissolved in aqueous solutions. Silicon was introduced into the solution using tetraethylorthosilicate. X-ray diffraction, infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy and transmission electron microscopy indicate that silicon doping induce different phase compositions and bioactivity of spherical- and whisker-like hydroxyapatite particles obtained under the same hydrothermal conditions. Silicon-substituted, spherical hydroxyapatites particles showed greater phase transformation to silicon-substituted α- calcium-phosphate compared with whiskers-like hydroxyapatite particles synthesized with the same amount of added silicon. Metabolic activity assay performed with SaOs2 osteosarcoma cells showed better biocompatibility of annealed biphasic spherical-like particles compared with annealed whiskerlike particles while dried spherical-like particles induce high cytotoxicity effect.
authors.affiliation: Faculty of Technology and Metallurgy, Karnegijeva 4, 11000, Belgrade, Serbia. bjokic@tmf.bg.ac.rs
authors.forename: B
authors.initials: B
authors.lastname: Jokic
authors.forename: M
authors.initials: M
authors.lastname: Mitric
authors.forename: M
authors.initials: M
authors.lastname: Popovic
authors.forename: L
authors.initials: L
authors.lastname: Sima
authors.forename: S M
authors.initials: SM
authors.lastname: Petrescu
authors.forename: R
authors.initials: R
authors.lastname: Petrovic
authors.forename: Dj
authors.initials: Dj
authors.lastname: Janackovic
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D045744:Cell Line, Tumor; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013314:Stress, Mechanical
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D017886:Durapatite; D012825:Silicon