title: In vitro evaluation of bacterial leakage along the implant-abutment interface of an external-hex implant after saliva incubation.
abstract: The aim of this in vitro study was to evaluate bacterial leakage along the implant-abutment interface under unloaded conditions. Twelve premachined abutments with plastic sleeves and 12 dental implants were used in this study. Prior to tests of bacterial leakage, samples from the inner parts of the implants were collected with sterile microbrushes to serve as negative controls for contamination. After casting, the abutments were tightened to 32 Ncm on the implants. The assemblies were immersed in 2.0 mL of human saliva and incubated for 7 days. After this period, possible contamination of the internal parts of the implants was evaluated using the DNA Checkerboard method. Microorganisms were found in the internal surfaces of all the implants evaluated. Aggregatibacter actinomycetemcomitans and Capnocytophaga gingivalis were the most incident species. No microorganisms were found in the samples recovered from the implants before contamination testing (negative control). Bacterial species from human saliva may penetrate the implant-abutment interface under unloaded conditions.
authors.affiliation: Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Brazil. cassionasc@forp.usp.br
authors.forename: Cássio
authors.initials: C
authors.lastname: do Nascimento
authors.forename: Paola Kirsten
authors.initials: PK
authors.lastname: Miani
authors.forename: Evandro
authors.initials: E
authors.lastname: Watanabe
authors.forename: Vinícius
authors.initials: V
authors.lastname: Pedrazzi
authors.forename: Rubens Ferreira
authors.initials: RF
authors.lastname: de Albuqerque
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification; D002206:Capnocytophaga / Q000235:genetics / Q000302:isolation & purification; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D000044:Dental Abutments / Q000009:adverse effects / Q000382:microbiology*; D059605:Dental Implant-Abutment Design / Q000009:adverse effects*; D015921:Dental Implants / Q000009:adverse effects / Q000382:microbiology*; D003763:Dental Leakage / Q000209:etiology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012463:Saliva / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D015921:Dental Implants