title: Triterpenoid saponins from Dipsacus asper and their activities in vitro.
abstract: Two new triterpenoid saponins 1 and 2, along with six known saponins 3-8, were isolated from the roots of Dipsacus asper. The structures of new compounds were established as 3-O-β-d-glucopyranosyl-(1 → 4)-[α-l-rhamnopyranosyl-(1 → 3)]-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 2)-α-l-arabinopyranosyl-hederagenin-28-O-β-d-glucopyranoside (dipsacus saponin J, 1) and 3-O-α-l-arabinopyranosyl-hederagenin-28-O-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranoside (dipsacus saponin K, 2). The structures were determined by extensive analysis of their spectroscopic data. Compounds 6 and 7 could significantly stimulate UMR106 cell proliferation and increase alkaline phosphatase activities in UMR106 cell at the concentration of 4 μM.
authors.affiliation: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plants Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liu
authors.forename: Xin-Luan
authors.initials: XL
authors.lastname: Wang
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Guo
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Pei-Gen
authors.initials: PG
authors.lastname: Xiao
authors.forename: Chao-Qing
authors.initials: CQ
authors.lastname: Huang
authors.forename: Li-Zhen
authors.initials: LZ
authors.lastname: Zheng
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.forename: Guang-Zhong
authors.initials: GZ
authors.lastname: Tu
mesh_terms: D000469:Alkaline Phosphatase / Q000187:drug effects; D049917:Dipsacaceae / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D012503:Saponins; C561958:dipsacus saponin J; C561959:dipsacus saponin K; D009828:Oleanolic Acid; D000469:Alkaline Phosphatase