title: Controlled delivery of gentamicin using poly(3-hydroxybutyrate) microspheres.
abstract: Poly(3-hydroxybutyrate), P(3HB), produced from Bacillus cereus SPV using a simple glucose feeding strategy was used to fabricate P(3HB) microspheres using a solid-in-oil-water (s/o/w) technique. For this study, several parameters such as polymer concentration, surfactant and stirring rates were varied in order to determine their effect on microsphere characteristics. The average size of the microspheres was in the range of 2 μm to 1.54 μm with specific surface areas varying between 9.60 m(2)/g and 6.05 m(2)/g. Low stirring speed of 300 rpm produced slightly larger microspheres when compared to the smaller microspheres produced when the stirring velocity was increased to 800 rpm. The surface morphology of the microspheres after solvent evaporation appeared smooth when observed under SEM. Gentamicin was encapsulated within these P(3HB) microspheres and the release kinetics from the microspheres exhibiting the highest encapsulation efficiency, which was 48%, was investigated. The in vitro release of gentamicin was bimodal, an initial burst release was observed followed by a diffusion mediated sustained release. Biodegradable P(3HB) microspheres developed in this research has shown high potential to be used in various biomedical applications.
authors.affiliation: Department of Applied and Molecular Biosciences, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK; E-Mails: lydiafrancis12@gmail.com (L.F.); keshavt@wmin.ac.uk (T.K.).
authors.forename: Lydia
authors.initials: L
authors.lastname: Francis
authors.forename: Decheng
authors.initials: D
authors.lastname: Meng
authors.forename: Jonathan
authors.initials: J
authors.lastname: Knowles
authors.forename: Tajalli
authors.initials: T
authors.lastname: Keshavarz
authors.forename: Aldo R
authors.initials: AR
authors.lastname: Boccaccini
authors.forename: Ipsita
authors.initials: I
authors.lastname: Roy
mesh_terms: D000327:Adsorption; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D005839:Gentamicins / Q000737:chemistry* / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D006885:Hydroxybutyrates / Q000737:chemistry*; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D004337:Drug Carriers; D005839:Gentamicins; D006885:Hydroxybutyrates; D011091:Polyesters; D013501:Surface-Active Agents; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates