title: Evaluation of hemicellulose removal by xylanase and delignification on SHF and SSF for bioethanol production with steam-pretreated substrates.
abstract: Steam-pretreated sweet sorghum bagasse (SSB) and Douglas-fir (DF) were employed for SHF and SSF to evaluate the effects of xylanase supplementation and delignification on ethanol production. Results indicated final ethanol concentration in SHF could reach 28.4 g/L (SSB) and 20.4 g/L (DF) by xylanase supplementation with the increase of 46% and 61% in comparison with controls. The delignification could significantly enhance final ethanol concentration to 31.2g/L (SSB) and 30.2 g/L (DF) with the increase of 61% and 138%. In SSF, final ethanol concentration in the delignified SSB and DF arrived at 27.6 g/L and 34.3 g/L with the increase of 26% and 157% compared with controls. However, only 2.2 g/L (SSB) and 6.9 g/L (DF) ethanol were obtained with xylanase supplementation. According to these results, it could be concluded that delignification was beneficial to improve ethanol production of SHF and SSF. The xylanase supplementation (0.12 g protein/g glucan) was only positive to SHF while retarded SSF seriously.
authors.affiliation: Provincial Key Laboratory of Agricultural Environmental Engineering, Sichuan Agricultural University-Chengdu Campus, Chengdu, Sichuan, PR China. fishensjtu@gmail.com
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.forename: Linoj
authors.initials: L
authors.lastname: Kumar
authors.forename: Jinguang
authors.initials: J
authors.lastname: Hu
authors.forename: Jack N
authors.initials: JN
authors.lastname: Saddler
mesh_terms: D056804:Biofuels; D002482:Cellulose / Q000737:chemistry*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism*; D028224:Pseudotsuga / Q000737:chemistry*; D013227:Steam
chemical_list: D056804:Biofuels; D011134:Polysaccharides; D013227:Steam; D000431:Ethanol; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse; D043364:Endo-1,4-beta Xylanases