title: High-throughput transposon mutagenesis of Corynebacterium glutamicum.
abstract: Construction of gene disruption mutants and analysis of the resultant phenotypes are an important strategy to study gene function. A simple and high-throughput method developed for microorganisms combines two different types of transposons, direct genomic DNA amplification and thermal asymmetric interlaced-PCR. The considerable utility of this approach is demonstrable in Corynebacterium glutamicum, where 18,000 transposon disruptants enabled the generation of an insertion library covering nearly 80% of the organism's 2,990 ORFs.
authors.affiliation: Research Institute of Innovative Technology for the Earth (RITE), Kizugawa-Shi, Kyoto, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Masayuki
authors.initials: M
authors.lastname: Inui
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yukawa
mesh_terms: D048230:Corynebacterium glutamicum / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial / Q000235:genetics; D016254:Mutagenesis, Insertional / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements