title: Identification of Stenotrophomonas maltophilia strains isolated from environmental and clinical samples: a rapid and efficient procedure.
abstract: OBJECTIVE
  Aim of the study is to identify accurately Stenotrophomonas maltophilia isolates recovered from environmental and clinical samples.
  
  
  RESULTS
  Recovery of Sten. maltophilia-like isolates from soil samples using the vancomycin, imipenem, amphotericin B (VIA) selective agar medium enabled distinction of various morphotype colonies. A set of soil and clinical isolates was tested for species identification using different methods. 16S rDNA analyses showed the dark green with a blue halo morphotype to be typical Sten. maltophilia strains. The API-20NE, Vitek-2 and Biolog phenotypic analyses typically used for the identification of clinical isolates did not perform well on these soil isolates. The species-specific PCR screening targeting Sten. maltophilia 23S rDNA and the multiplex smeD/ggpS PCR, differentiating Sten. maltophilia from Stenotrophomonas rhizophila, were tested for improvement of these identification schemes. The latter multiplex PCR identified all isolates tested in this study, whatever be their origin.
  
  
  CONCLUSIONS
  Isolation on VIA medium and confirmation of Sten. maltophilia species membership by smeD PCR is proposed to identify environmental and clinical isolates of Sten. maltophilia.
  
  
  CONCLUSIONS
  The proposed approach enables isolation and identification of Sten. maltophilia from different environments in an easy and rapid way. This approach will be useful to accurately manage studies on the abundance and distribution of Sten. maltophilia in hospital and nonhospital environments.
authors.affiliation: Université de Lyon, France Research Group on 'Bacterial Opportunistic Pathogens and Environment', UMR 5557 Ecologie Microbienne, CNRS, VetAgro Sup and Université Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Pinot
authors.forename: A
authors.initials: A
authors.lastname: Deredjian
authors.forename: S
authors.initials: S
authors.lastname: Nazaret
authors.forename: E
authors.initials: E
authors.lastname: Brothier
authors.forename: B
authors.initials: B
authors.lastname: Cournoyer
authors.forename: C
authors.initials: C
authors.lastname: Segonds
authors.forename: S
authors.initials: S
authors.lastname: Favre-Bonté
mesh_terms: D000362:Agar / Q000737:chemistry; D000666:Amphotericin B; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D015378:Imipenem; D060885:Multiplex Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D020615:Stenotrophomonas maltophilia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D014640:Vancomycin
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D014640:Vancomycin; D015378:Imipenem; D000666:Amphotericin B; D000362:Agar