title: Protective effects of ω-conotoxin on amyloid-β-induced damage in PC12 cells.
abstract: The present study was designed to investigate the potential neuroprotective effect of ω-conotoxin (ω-CTX) in a cell-based model of Alzheimer's disease (AD) using cultured PC12 cells incubated with β-amyloid (Aβ). Immunohistochemical staining, Western blot, MTT and TdT-mediated dUTP-biotin nick end labeling (TUNEL) analysis were employed to assess the cell viability and cell death. Aβ in this model was clearly neurotoxic, inducing necrosis and apoptosis. ω-CTX antagonized the effects of Aβ: there was an increase in cell viability and a suppression of inflammatory- and oxidative stress-related factors. These data suggest that ω-CTX may have neuroprotective actions against Aβ-induced neurotoxicity. The significance of these new findings relative to the etiology and treatment of AD is discussed.
authors.affiliation: Institute of Anatomy and Cell Biology, Medical College, Zhejiang University, Hangzhou, China.
authors.forename: Marong
authors.initials: M
authors.lastname: Fang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Han
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Hu
authors.forename: Jin-biao
authors.initials: JB
authors.lastname: Zhan
authors.forename: Shucai
authors.initials: S
authors.lastname: Ling
authors.forename: John A
authors.initials: JA
authors.lastname: Rudd
authors.forename: Yu
authors.initials: Y
authors.lastname: Geng
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D008858:Microscopy, Phase-Contrast; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D051381:Rats; D014343:Trypan Blue / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000032:analysis; D020960:omega-Conotoxins / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; C494828:Bax protein, rat; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020960:omega-Conotoxins; D053148:Caspase 3; D014343:Trypan Blue