title: BLCAP induces apoptosis in human Ewing's sarcoma cells.
abstract: Bladder cancer-associated protein (BLCAP) is a novel candidate tumor suppressor gene identified from human bladder carcinoma and highly associated with the invasion of bladder cancer. We previously reported that it also plays a key role in the tumorigenesis and metastasis of human osteosarcoma. In the present study, we constructed a recombinant encoding BLCAP cDNA. Overexpression of BLCAP resulted in growth inhibition and induced apoptosis of human TC-135 Ewing's sarcoma cells in vitro. We further investigated the caspase-3/7 activity and expressions of the fusion transcription factor Ewing's sarcoma protein-friend leukemia virus integration 1 (EWS-FLI1) and the apoptosis regulator B-cell lymphoma 2 (BCL-2). Cell apoptosis was accompanied by the down-regulated expression of EWS-FLI1 and BCL-2. Our present results suggest that BLCAP may play a role not only in regulating cell proliferation but also in coordinating apoptosis through the down-regulation of BCL-2 and EWS-FLI1 in human Ewing's sarcoma cells.
authors.affiliation: Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital, Xi-an, China.
authors.forename: De-Gang
authors.initials: DG
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Ding
authors.forename: Ming-Mei
authors.initials: MM
authors.lastname: Wu
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Fan
authors.forename: Katsuji
authors.initials: K
authors.lastname: Shimizu
authors.forename: Taikoh
authors.initials: T
authors.lastname: Dohjima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nozawa
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Wakahara
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Ohno
authors.forename: Yun-Shan
authors.initials: YS
authors.lastname: Guo
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Ma
authors.forename: Jian-Li
authors.initials: JL
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000494:pharmacology*; D015514:Oncogene Proteins, Fusion / Q000096:biosynthesis; D051789:Proto-Oncogene Protein c-fli-1 / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D034802:RNA-Binding Protein EWS / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012512:Sarcoma, Ewing / Q000378:metabolism*
chemical_list: C453739:BLCAP protein, human; C106031:EWS-FLI fusion protein; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D051789:Proto-Oncogene Protein c-fli-1; D019253:Proto-Oncogene Proteins c-bcl-2; D034802:RNA-Binding Protein EWS; D011994:Recombinant Proteins; D053148:Caspase 3; D053179:Caspase 7