title: Lycopene and apo-10'-lycopenal do not alter DNA methylation of GSTP1 in LNCaP cells.
abstract: DNA hypermethylation and silencing of tumor-suppressor genes are commonly seen in human cancers, and likely contribute to the process of carcinogenesis. A growing body of evidence suggests that dietary compounds may alter cancer risk through epigenetic modifications. Glutathione S-transferase P1 (GSTP1) is hypermethylated in >90% of prostate cancer cases making it one of the most common genome alterations in prostate cancer. LNCaP cells were treated either with lycopene or apo-10'-lycopenal for 7days, and mRNA expression and DNA methylation of GSTP1 were evaluated. Neither compound significantly altered expression nor methylation of GSTP1 while treatment with 5-azacytidine decreased methylation by 78%. These findings demonstrate that lycopene and apo-10'-lycopenal are not effective demethylating agents of GSTP1 in the human LNCaP cell line.
authors.affiliation: Division of Nutritional Sciences and Department of Food Science and Human Nutrition, 905 S. Goodwin Avenue, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Ann G
authors.initials: AG
authors.lastname: Liu
authors.forename: John W
authors.initials: JW
authors.lastname: Erdman
mesh_terms: D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000187:drug effects*; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D006801:Humans; D000077276:Lycopene; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*
chemical_list: D002338:Carotenoids; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D000077276:Lycopene