title: Cytotoxic and haemolytic steroidal glycosides from the Caribbean sponge Pandaros acanthifolium.
abstract: Six new steroidal saponins, pandarosides K-M (1-3) and their methyl esters (4-6), were isolated as minor components, after a careful chemical reinvestigation of the Caribbean sponge Pandaros acanthifolium. Their structures were established on the basis of spectroscopic analyses and comparison with the data obtained from previous metabolites of this family. All new compounds showed moderate to weak activity against four parasitic protozoa. Additionally, these compounds and previously reported pandarosides and acanthifoliosides were tested on three human tumour cell lines, and their haemolytic and liposome permeabilizing activity were assessed. Two pandarosides exhibited moderate to strong cytotoxic effect, while three acanthifoliosides showed strong haemolytic activity.
authors.affiliation: Laboratoire de Chimie des Molécules Bioactives et Arômes, UMR 6001 CNRS, University of Nice-Sophia Antipolis, Faculté des Sciences Parc Valrose, 06108 Nice Cedex 2, France. erikluis18@gmail.com
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.forename: Tom
authors.initials: T
authors.lastname: Turk
authors.forename: Deniz
authors.initials: D
authors.lastname: Tasdemir
authors.forename: Manca
authors.initials: M
authors.lastname: Gorjanc
authors.forename: Marcel
authors.initials: M
authors.lastname: Kaiser
authors.forename: Olivier P
authors.initials: OP
authors.lastname: Thomas
authors.forename: Rogelio
authors.initials: R
authors.lastname: Fernández
authors.forename: Philippe
authors.initials: P
authors.lastname: Amade
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000737:chemistry / Q000494:pharmacology*; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017691:Caribbean Region; D045744:Cell Line, Tumor; D053878:Hemolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011161:Porifera / Q000737:chemistry*; D051381:Rats; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D000981:Antiprotozoal Agents; D053878:Hemolytic Agents; D012503:Saponins; D013256:Steroids