title: [UPLC-MS analysis of constituents of dachuanxiong fang active parts absorbed into blood].
abstract: OBJECTIVE
  To study the constituents absorbed into blood following the intragstric administration of Dachuanxiong Fang active parts(DCXF) to the experimental migrainous model rats induced by nitroglycerin.
  
  
  METHODS
  The UPLC-MS was used as the analytic method. The DCXF, Chuanxiong active parts, Tianma active parts, gastrodin, ferulic acid and senkyunolide I were used as the control samples. The information on the total ion chromatogram, mass chromatogram and the mass spectrogram were synthetically analyzed to confirm the constituents absorbed into blood.
  
  
  RESULTS
  Ten of the DCXF constituents were detected in the rats plasma post the intragastric administration of DCXF, in whitch four including gastrodin came from Tianma active parts and six including ferulic acid and senkyunolide I from Chuanxiong active parts.
  
  
  CONCLUSIONS
  The findings abtained from the study can provide the useful information for the determination of bioactive substances of the DCXF.
authors.affiliation: College of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China. weiyuanfengyuer@yeah.net
authors.forename: Yuanfeng
authors.initials: Y
authors.lastname: Wei
authors.forename: Xiao
authors.initials: X
authors.lastname: Lin
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000097:blood; D001592:Benzyl Alcohols / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000097:blood; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001572:Benzofurans; D001592:Benzyl Alcohols; D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C045345:gastrodin; C045855:3-N-butyl-4,5-dihydrophthalide; C004999:ferulic acid