title: [Immunological efficiency induced by HIV-1 p24 DNA combined with P24 protein].
abstract: New strategies to improve vaccine efficacy against human immunodeficiency virus type 1 (HIV-1) are still required. DNA vaccines, exhibiting potential advantages over conventional vaccines for their simplicity and versatility, can induce specific humoral and cellular immune responses. We developed a recombinant pVAX1 DNA vector carrying p24 gene of HIV-1. The results showed that pVAX1 mediated gene possessed the ability of effective expression in both transfected 293T cells and BALB/c mice. And pVAX1-p24 DNA prime and boost immunization can induce significant P24-specific humoral immune responses and cellular immune responses in BALB/c mice. Furthermore, immunization with pVAX1-p24 DNA prime and protein boost induced 7.3 to 8.0-fold greater p24-specific humoral responses than pVAX1-p24 DNA prime and boost, while the cellular immune responses induced by combined immunization was lower. The results suggested that pVAX1-p24 DNA and P24 protein vaccine is a promising HIV-1 vaccine, and the selections of the immunization strategies are important for the immunization results.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Chen
authors.forename: Shijuan
authors.initials: S
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Song
authors.forename: Wenlin
authors.initials: W
authors.lastname: Huang
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D004247:DNA / Q000235:genetics / Q000276:immunology*; D057809:HEK293 Cells; D016655:HIV Core Protein p24 / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D016655:HIV Core Protein p24; D019444:Vaccines, DNA; C514799:p24 protein, Human Immunodeficiency Virus Type 1; D004247:DNA