title: The inhibition activity of selected beta-carboline alkaloids on enzymes of acetylcholinesterase and butyrylcholinesterase.
abstract: This thesis deals with testing of inhibition activity beta-carboline alkaloids on activity of enzymes acetylcholinesterase (ACHE) and butyrylcholinesterase (BUCHE) using test "Fast Blue B salt" at TLC desk and Ellman's test using spectrophotometer. It was also investigated how dimethylsulfoxide used as a solvent in combination with water affects activity of enzymes and alkaloids. Results show harmine in form of base and salt in water and in mixture of DMSO and water has the hightest inhibition activity on ACHE using eserine as reference substance. Harmalol in form of salt in water and harmine in form of base and salt in mixture of DMSO and water has the hightest activity on BUCHE. It was find out that DMSO considerably affects activity of enzymes and alkaloids.
authors.affiliation: Department of Pharmacognosy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Krsková
authors.forename: Jan
authors.initials: J
authors.lastname: Martin
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dusek
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000470:Alkaloids / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000378:metabolism*; D002243:Carbolines / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D002243:Carbolines; D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D004121:Dimethyl Sulfoxide