title: Somatostatin receptor 5 is palmitoylated by the interacting ZDHHC5 palmitoyltransferase.
abstract: Many G-protein coupled receptors are palmitoylated in their C-terminal, intracellular regions. So far no enzymes responsible for this modification have been described. We identified an interaction of the membrane proximal helix 8 of somatostatin receptor 5 (SSTR5) with the N-terminal region of the putative palmitoyltransferase ZDHHC5 using the Ras recruitment interaction screening system. ZDHHC5 and SSTR5 are colocalized at the plasma membrane and can be efficiently coimmunoprecipitated from transfected cells. Coexpression of ZDHHC5 in HEK293 cells increased palmitoylation of SSTR5 whereas knock-down of endogenous ZDHHC5 by siRNAs decreased it. Our data identify the first palmitoyltransferase for a G-protein coupled receptor.
authors.affiliation: Institut für Humangenetik, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Tarja
authors.initials: T
authors.lastname: Kokkola
authors.forename: Claudia
authors.initials: C
authors.lastname: Kruse
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Roy-Pogodzik
authors.forename: Jenna
authors.initials: J
authors.lastname: Pekkinen
authors.forename: Carola
authors.initials: C
authors.lastname: Bauch
authors.forename: Hans-Hinrich
authors.initials: HH
authors.lastname: Hönck
authors.forename: Hanjo
authors.initials: H
authors.lastname: Hennemann
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Kreienkamp
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D054878:Lipoylation; D008856:Microscopy, Fluorescence; D034741:RNA, Small Interfering; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D017481:Receptors, Somatostatin; C094573:somatostatin receptor 5; D000217:Acyltransferases