title: Transient increase of free iron in rat livers following hemorrhagic-traumatic shock and reperfusion is independent of heme oxygenase 1 upregulation.
abstract: Hemorrhagic-traumatic shock (HTS) followed by reperfusion induces heme oxygenase (HO) 1. Free iron (Fe2+) may cause oxidative stress, if not adequately sequestered. We aimed to characterize HO-1-mediated effects on Fe2+ levels in liver and transferrin-bound iron (TFBI) in plasma following HTS, including laparotomy, bleeding, and inadequate and adequate reperfusion. Anesthetized rats showed upregulated HO-1 mRNA at 40 min after HTS, which was followed by increased HO activity at 3 h after shock. Fe2+ levels were transiently increased at 40 min after shock, a time point when HO activity was not affected yet. Levels of plasma TFBI were higher in HTS animals, showing the highest levels at 40 min after shock, and decreased thereafter. In addition, we modulated HO activity 6 h before HTS by administering an inhibitor (zinc-protoporphyrin IX) or an activator (hemin) of HO. At 18 h after HTS in all shock groups, HO activity was increased, the highest being in the hemin-pretreated group. The zinc-protoporphyrin IX-treated HTS animals showed increased HO-1 mRNA and Fe2+ levels in the liver compared with the untreated HTS animals. Transferrin-bound iron levels were affected by pharmacological modulation before shock. All animals undergoing HTS displayed increased TFBI levels after reperfusion; however, in animals pretreated with hemin, TFBI levels increased less. Our data indicate that increase in Fe2+ levels in liver and plasma early after HTS is not mediated by HO-1 upregulation, but possibly reflects an increased mobilization from internal iron stores or increased cell damage. Thus, upregulation of HO activity by hemin does not increase Fe2+ levels following HTS and reperfusion.
authors.affiliation: Department of Biomedical Sciences, University of Veterinary Medicine, Veterinaerplatz 1, Vienna, Austria.
authors.forename: Astrid
authors.initials: A
authors.lastname: Postl
authors.forename: Clara
authors.initials: C
authors.lastname: Zifko
authors.forename: Romana T
authors.initials: RT
authors.lastname: Hartl
authors.forename: Thomas
authors.initials: T
authors.lastname: Ebel
authors.forename: Ingrid
authors.initials: I
authors.lastname: Miller
authors.forename: Rudolf
authors.initials: R
authors.lastname: Moldzio
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Kozlov
authors.forename: Soheyl
authors.initials: S
authors.lastname: Bahrami
authors.forename: Johanna Catharina
authors.initials: JC
authors.lastname: Duvigneau
mesh_terms: D000818:Animals; D015153:Blotting, Western; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012771:Shock, Hemorrhagic / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D007501:Iron; D051547:Heme Oxygenase-1