title: Effect of Candida antarctica lipase B immobilization on the porous structure of the carrier.
abstract: A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-fold) and the specific volume (up to 2.1-fold) of the epoxy resin. A similar behaviour is observed for glutaraldehyde-modified epoxy resins. The influences of the resin porosity properties on the loading of Candida antarctica lipase B during immobilization and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized lipase are studied.
authors.affiliation: Polymer Chemistry Department, Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands.
authors.forename: Nemanja
authors.initials: N
authors.lastname: Miletić
authors.forename: Zorica
authors.initials: Z
authors.lastname: Vuković
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nastasović
authors.forename: Katja
authors.initials: K
authors.lastname: Loos
mesh_terms: D000327:Adsorption; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000737:chemistry / Q000201:enzymology*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004853:Epoxy Resins / Q000737:chemistry; D005026:Ethylene Glycols; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D005976:Glutaral / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D008689:Methacrylates / Q000737:chemistry*; D009596:Nitrophenols / Q000378:metabolism; D010316:Particle Size; D016062:Porosity
chemical_list: D004800:Enzymes, Immobilized; D004853:Epoxy Resins; D005026:Ethylene Glycols; D005656:Fungal Proteins; D008689:Methacrylates; D009596:Nitrophenols; C526534:poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate); C008642:4-nitrophenyl acetate; D008049:Lipase; C431349:lipase B, Candida antarctica; D005976:Glutaral