title: Immunogenicity of Leishmania donovani iron superoxide dismutase B1 and peroxidoxin 4 in BALB/c mice: the contribution of Toll-like receptor agonists as adjuvant.
abstract: In this study, we assessed the immune response of two Leishmania donovani recombinant proteins: iron superoxide dismutase B1 (SODB1) and peroxidoxin 4 (Pxn4) in BALB/c mice. Assessment of the immunogenicity of these proteins alone or combined with Toll-like receptor 9 (TLR-9) agonist (CpG ODN) or TLR-4 agonist (GLA-SE) showed that they elicit specific antibody as well as cytokine production in response to the respective antigen in vitro. The use of adjuvants augmented immunogenicity of these antigens and more importantly, skewed the immune response to a Th1-type. These results indicate that recombinant SODB1 and Pxn4 proteins are potential vaccine candidates when administered with appropriate adjuvants.
authors.affiliation: University of Calgary, Department of Biological Sciences, Room 374, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4.
authors.forename: Nada S
authors.initials: NS
authors.lastname: Daifalla
authors.forename: A Genetu
authors.initials: AG
authors.lastname: Bayih
authors.forename: Lashitew
authors.initials: L
authors.lastname: Gedamu
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D007893:Leishmania donovani / Q000235:genetics / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D054464:Peroxiredoxins / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology*; D051193:Toll-Like Receptors / Q000819:agonists*
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D051193:Toll-Like Receptors; D054464:Peroxiredoxins; D013482:Superoxide Dismutase