title: Analytical investigations of toxic p-phenylenediamine (PPD) levels in clinical urine samples with special focus on MALDI-MS/MS.
abstract: Para-phenylenediamine (PPD) is a common chromophoric ingredient in oxidative hair-dyes. In some African countries like Sudan, Egypt and Morocco but also in India this chemical is used alone or in combination with colouring extracts like Henna for dyeing of the hair or the skin. Excessive dermal exposure to PPD mainly leads to the N-mono- and N,N'-diacetylated products (MAPPD, DAPPD) by N-acetyltransferase 1 and 2 (NAT1 and 2) catalyzed reactions. Metabolites and PPD are mainly excreted via renal clearance. Despite a low risk of intoxication when used in due form, there are numerous cases of acute intoxication in those countries every year. At the ENT Hospital - Khartoum (Sudan) alone more than 300 cases are reported every year (~10% fatal), mostly caused by either an accidental or intended (suicidal) high systemic exposure to pure PPD. Intoxication leads to a severe clinical syndrome including laryngeal edema, rhabdomyolysis and subsequent renal failure, neurotoxicity and acute toxic hepatitis. To date, there is no defined clinical treatment or antidote available and treatment is largely supportive. Herein, we show the development of a quick on-site identification assay to facilitate differential diagnosis in the clinic and, more importantly, the implementation of an advanced analytical platform for future in-depth investigations of PPD intoxication and metabolism is described. The current work shows a sensitive (~25 µM) wet chemistry assay, a validated MALDI-MS/MS and HPLC-UV assay for the determination of PPD and its metabolites in human urine. We show the feasibility of the methods for measuring PPD over a range of 50-1000 µM. The validation criteria included linearity, lower limit of quantification (LLOQ), accuracy and precision, recovery and stability. Finally, PPD concentrations were determined in clinical urine samples of cases of acute intoxication and the applied technique was expanded to identify MAPPD and DAPPD in the identical samples.
authors.affiliation: Department of Neurology, Laboratory of Neuro-Oncology and Clinical and Cancer Proteomics, University Medical Center Rotterdam, ErasmusMC, Rotterdam, The Netherlands. g.hooff@erasmusmc.nl
authors.forename: Gero P
authors.initials: GP
authors.lastname: Hooff
authors.forename: Nick A
authors.initials: NA
authors.lastname: van Huizen
authors.forename: Roland J W
authors.initials: RJ
authors.lastname: Meesters
authors.forename: Eduard E
authors.initials: EE
authors.lastname: Zijlstra
authors.forename: Mohamed
authors.initials: M
authors.lastname: Abdelraheem
authors.forename: Waleed
authors.initials: W
authors.lastname: Abdelraheem
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hamdouk
authors.forename: Jan
authors.initials: J
authors.lastname: Lindemans
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
mesh_terms: D001191:Arylamine N-Acetyltransferase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D057230:Limit of Detection; D010655:Phenylenediamines / Q000378:metabolism / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004396:Coloring Agents; D007527:Isoenzymes; D010655:Phenylenediamines; D001191:Arylamine N-Acetyltransferase; C089384:N-acetyltransferase 1; C478900:NAT2 protein, human; C029728:4-phenylenediamine