title: Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins.
abstract: In this report, we developed a universal assay method for both genomic DNA and proteins by using enzyme-based multi-component optical nanoprobes. The nanoprobes are gold nanoparticles assembled with bio-recognizing and signaling elements. We firstly demonstrated that the nanoprobes could detect unpurified asymmetric polymerase chain reaction (PCR) product from genomic DNA of Escherichia coli, with the sensitivity approximately 10 times higher than that of quantitative real-time PCR assay. The limit of detection (LOD) of our nanoprobe-based method is less than 10 pg template DNA (target DNA). Using DNA aptamers as recognition elements, we also showed that as few as 0.1 nM thrombin could be colorimetrically detected with high specificity. These results indicated that the enzyme-based multi-component nanoprobes have the capability to work with real biological samples, and have the potential in various biological and clinical applications.
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D009841:Oligonucleotides; D011506:Proteins; D006046:Gold; D004247:DNA; D013917:Thrombin