title: [Expression and function of autophagy after ischemia/reperfusion in rats hippocampus neuron].
abstract: OBJECTIVE
  To explore the expression of autophagy after ischemia/reperfusion and its possible function in rats hippocampus neurons.
  
  
  METHODS
  After 2 hours oxygen-glucose deprivation and different periods time of reperfusion (OGD/R) treatment in primary hippocampal neurons, neuron viability was evaluated by MTT assay, specific structure of autophagosome and specific protein of autophagy microtubule-associated protein 1 light chain 3 B (LC3B) were detected by transmission electron microscope and immunofluorescence respectively. The inhibitor of autophagy 3-Methyladenine (3-MA) was also used to exam the viability of neurons.
  
  
  RESULTS
  Treatment by OGD/R markedly reduced neuronal viability. Compared to the control group, autophagy existed in different time periods after OGD/R shown both in transmission electron microscope and immunofluorescence. Application of 3-MA significantly reduced neuronal viability.
  
  
  CONCLUSIONS
  Oxygen-glucose deprivation can activate autophagy in rat hippocampus neurons, which may resist the injury during ischemia/reperfusion.
authors.affiliation: Department of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Su
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Jiang
authors.forename: De-Qing
authors.initials: DQ
authors.lastname: Peng
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Gao
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Liu
authors.forename: Ling-Bo
authors.initials: LB
authors.lastname: Qian
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Ye
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xia
mesh_terms: D000818:Animals; D001343:Autophagy / Q000502:physiology*; D002545:Brain Ischemia / Q000473:pathology*; D015687:Cell Hypoxia; D016895:Culture Media, Serum-Free; D006624:Hippocampus / Q000166:cytology* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000473:pathology*; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology*
chemical_list: D016895:Culture Media, Serum-Free