title: Malaria parasites (Plasmodium spp.) infecting introduced, native and endemic New Zealand birds.
abstract: Avian malaria is caused by intracellular mosquito-transmitted protist parasites in the order Haemosporida, genus Plasmodium. Although Plasmodium species have been diagnosed as causing death in several threatened species in New Zealand, little is known about their ecology and epidemiology. In this study, we examined the presence, microscopic characterization and sequence homology of Plasmodium spp. isolates collected from a small number of New Zealand introduced, native and endemic bird species. We identified 14 Plasmodium spp. isolates from 90 blood or tissue samples. The host range included four species of passerines (two endemic, one native, one introduced), one species of endemic pigeon and two species of endemic kiwi. The isolates were associated into at least four distinct clusters including Plasmodium (Huffia) elongatum, a subgroup of Plasmodium elongatum, Plasmodium relictum and Plasmodium (Noyvella) spp. The infected birds presented a low level of peripheral parasitemia consistent with chronic infection (11/15 blood smears examined). In addition, we report death due to overwhelming parasitemia in a blackbird, a great spotted kiwi and a hihi. These deaths were attributed to infections with either Plasmodium spp. lineage LINN1 or P. relictum lineage GRW4. To the authors' knowledge, this is the first published report of Plasmodium spp. infection in great spotted and brown kiwi, kereru and kokako. Currently, we are only able to speculate on the origin of these 14 isolates but consideration must be made as to the impact they may have on threatened endemic species, particularly due to the examples of mortality.
authors.affiliation: Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11-222, Palmerston North 4442, New Zealand. L.Howe@massey.ac.nz
authors.forename: Laryssa
authors.initials: L
authors.lastname: Howe
authors.forename: Isabel C
authors.initials: IC
authors.lastname: Castro
authors.forename: Ellen R
authors.initials: ER
authors.lastname: Schoener
authors.forename: Stuart
authors.initials: S
authors.lastname: Hunter
authors.forename: Rosemary K
authors.initials: RK
authors.lastname: Barraclough
authors.forename: Maurice R
authors.initials: MR
authors.lastname: Alley
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000469:parasitology*; D001717:Birds; D001769:Blood / Q000469:parasitology; D002908:Chronic Disease; D016000:Cluster Analysis; D045303:Cytochromes b / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D008288:Malaria / Q000469:parasitology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009520:New Zealand; D018512:Parasitemia / Q000469:parasitology / Q000662:veterinary; D010802:Phylogeny; D010961:Plasmodium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D045303:Cytochromes b