title: Preparation of fibrous cellulose by enzymatic polymerization using cross-linked mutant endoglucanase II.
abstract: A cross-linked mutant endoglucanase II was prepared for enzymatic polymerization to cellulose. The cross-linked enzyme is composed of three mutant enzymes showing polymerization activity. A characteristic feature of the polymerization with this cross-linked enzyme is formation of cellulose fibrils in contrast to plate-like crystals obtained by using a free enzyme.
authors.affiliation: Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Itsuko
authors.initials: I
authors.lastname: Nakamura
authors.forename: Akira
authors.initials: A
authors.lastname: Makino
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Horikawa
authors.forename: Junji
authors.initials: J
authors.lastname: Sugiyama
authors.forename: Masashi
authors.initials: M
authors.lastname: Ohmae
authors.forename: Shunsaku
authors.initials: S
authors.lastname: Kimura
mesh_terms: D055162:Biocatalysis; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D058105:Polymerization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002482:Cellulose; C056373:endoglucanase 2; D002480:Cellulase