title: Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of olaquindox residues.
abstract: A rapid immunochromatographic lateral flow test strip of competitive format has been developed for the specific determination of olaquindox (OLA) residues in pig urine and muscle tissues. The sensitivity of the test strip was found to be 1.58 ± 0.27 μg/kg and 1.70 ± 0.26 μg/kg of OLA in pig urine and muscle tissues, and the lower detection limit was 0.27 ± 0.08 μg/kg and 0.31 ± 0.07 μg/kg respectively. For negative pig urine and muscle samples spiked with 4, 12, and 36 μg/kg, the recovery range was 83.0-94.0% and 78.8-87.4% and the coefficient of variation scope [CV (%)] was 3.17-7.41% and 4.66-7.64% respectively. Parallel analysis of OLA samples from pig urine and muscle tissue showed comparable results from the test strip and HPLC. Each test requires 5-8 min, and the test strip can provide a useful screening method for quantitative, semiquantitative, or qualitative detection of OLA residues.
authors.affiliation: Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Song
authors.forename: Qingtang
authors.initials: Q
authors.lastname: Liu
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhi
authors.forename: Jifei
authors.initials: J
authors.lastname: Yang
authors.forename: Yubao
authors.initials: Y
authors.lastname: Zhi
authors.forename: Qingmei
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
authors.forename: Ruiguang
authors.initials: R
authors.lastname: Deng
authors.forename: Justin
authors.initials: J
authors.lastname: Casas
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D017979:Gold Colloid; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000737:chemistry; D011810:Quinoxalines / Q000032:analysis* / Q000652:urine; D011934:Reagent Strips; D012680:Sensitivity and Specificity; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011810:Quinoxalines; D011934:Reagent Strips; D019155:Veterinary Drugs; C013168:olaquindox