title: Study on the interaction of amino phosphine ester derivatives with DNA by spectroscopy, modeling and calorimetry.
abstract: The binding properties of amino phosphate ester derivatives, compound 1 and 2 with calf thymus DNA (CT-DNA) were investigated by UV spectra, fluorescence spectra, molecular modeling and isothermal titration calorimetry (ITC). The intrinsic binding constants K(b) of compound 1 and 2 with CT-DNA were determined by fluorescence spectroscopy and ITC, respectively. The results indicated that the two compounds bind to CT-DNA with different binding affinity, which is in the order of compound 1>compound 2. At the same time, fluorescence spectra suggested that the mechanism of the binding of the two compounds to CT-DNA is a static enhancing type. According to the ethidium bromide displacement experiments, UV spectra, molecular modeling and ITC studies, it can be concluded that compound 1 and 2 are intercalators that can slide into the G-C rich region of CT-DNA. Furthermore, ITC data showed that compound/DNA binding is enthalpy controlled.
authors.affiliation: College of Chemistry and Environmental Science, Henan Normal University, Xinxiang, Henan 453007, PR China. yanlu2001@sohu.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Gongke
authors.initials: G
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Tang
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Hao
authors.forename: Meihua
authors.initials: M
authors.lastname: Xu
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D002151:Calorimetry / Q000379:methods; D004247:DNA / Q000737:chemistry*; D004952:Esters / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry; D006358:Hot Temperature; D007364:Intercalating Agents / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D008968:Molecular Conformation; D010720:Phosphines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013816:Thermodynamics
chemical_list: D004952:Esters; D007364:Intercalating Agents; D010720:Phosphines; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium