title: Effect of acid blue 80, an anthracenedione dye, on rainbow trout liver, gill and gut cells in vitro.
abstract: Acid Blue 80 (AB80) is a dark blue colorant that like other synthetic dyes can get into the environment. Cultures of rainbow trout cell lines were dosed with AB80 either directly, which involved mixing AB80 stock solution into the medium over cells, or indirectly, which involved replacing the medium in cultures with medium that had AB80. A dose-dependent decline in cell viability was found in cultures with or without fetal bovine serum (FBS) after direct dosing. However, for FBS cultures, indirect dosing caused no loss of viability over 24h and in the long term was detrimental to RTgill-W1 but not RTL-W1 cultures. After 6 days at 50mg/L cytotoxicity was evident and by 9 days RTgill-W1 cell number had declined. Yet AB at 1mg/L elicited no changes over 9 days in any cell line. AB80 appears to have the potential to be toxic at only very high concentrations.
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, Ont., Canada N2L 3G1.
authors.forename: P N Catherine
authors.initials: PN
authors.lastname: Tee
authors.forename: Y T Janice
authors.initials: YT
authors.lastname: Wong
authors.forename: James P
authors.initials: JP
authors.lastname: Sherry
authors.forename: Niels C
authors.initials: NC
authors.lastname: Bols
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000166:cytology / Q000187:drug effects*; D005880:Gills / Q000166:cytology / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D017686:Oncorhynchus mykiss
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D016895:Culture Media, Serum-Free; D019363:Cytochrome P-450 CYP1A1; C433873:acid blue 80