title: Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development.
abstract: Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits. Our results suggested the occurrence of a metabolic change during citrus fruit maturation, where the organic acid and amino acid accumulation seen during the early stages of development shifted into sugar synthesis during the later stage of citrus fruit development. The expression of invertases remained unchanged, while an invertase inhibitor was up-regulated towards maturation. The increased expression of sucrose-phosphate synthase and sucrose-6-phosphate phosphatase and the rapid sugar accumulation suggest that sucrose is also being synthesized in citrus juice sac cells during the later stage of fruit development.
authors.affiliation: Department of Plant Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Ehud
authors.initials: E
authors.lastname: Katz
authors.forename: Kyung Hwan
authors.initials: KH
authors.lastname: Boo
authors.forename: Ho Youn
authors.initials: HY
authors.lastname: Kim
authors.forename: Richard A
authors.initials: RA
authors.lastname: Eigenheer
authors.forename: Brett S
authors.initials: BS
authors.lastname: Phinney
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shulaev
authors.forename: Florence
authors.initials: F
authors.lastname: Negre-Zakharov
authors.forename: Avi
authors.initials: A
authors.lastname: Sadka
authors.forename: Eduardo
authors.initials: E
authors.lastname: Blumwald
mesh_terms: D002957:Citrus / Q000254:growth & development* / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase; D010744:Phosphoric Monoester Hydrolases; C022697:sucrose-phosphatase