title: Signal profile on Gd-EOB-DTPA-enhanced MR imaging in non-alcoholic steatohepatitis and liver cirrhosis induced in rats: correlation with transporter expression.
abstract: OBJECTIVE
  To compare the transporter expression and signal profile on Gd-EOB-DTPA-enhanced MRI between non-alcoholic steatohepatitis (NASH) and cirrhotic liver induced in rats, and investigate the correlation of the transporter expression and fibrosis rate in both diseases.
  
  
  METHODS
  Forty-eight rats were divided into four groups of 12: TAA (cirrhosis), NASH 7- and 10-week, and control groups. Each group was divided into two subgroups: Group 1 for MRI and Group 2 for transporter examinations.
  
  
  RESULTS
  The relative enhancement of the TAA group was significantly lower than those of other groups (p < 0.01). The T(max) and T(1/2) of the NASH 10-week group was significantly prolonged in comparison with the TAA group (p < 0.01). There was no significant difference in the oatp1 expression, whereas the mrp2 expression of the TAA group was significantly higher than those of other groups (p < 0.01). There was no significant correlation between the fibrosis rate and oatp1 expression, whereas a paradoxical correlation was found between the fibrosis rate and mrp2 expression (NASH: negative correlation, r = 0.91, p < 0.01; TAA: positive correlation, r = 0.85, p < 0.01).
  
  
  CONCLUSIONS
  Our findings showed that the mrp2 expression in cirrhosis increases in comparison with NASH, and there was a paradoxical correlation between the fibrosis rate and mrp2 expression.
authors.affiliation: Diagnostic Imaging Medical Affairs, Medical Affairs, Bayer Yakuhin, Ltd., 4-9, Umeda 2-chome, Kita-ku, Osaka, Osaka 530-0001, Japan. natsuko.tsuda@bayer.com
authors.forename: Natsuko
authors.initials: N
authors.lastname: Tsuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Matsui
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D003287:Contrast Media; D005234:Fatty Liver / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D019786:Gadolinium DTPA; D007091:Image Processing, Computer-Assisted; D008106:Liver Cirrhosis, Experimental / Q000175:diagnosis / Q000378:metabolism*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease; D029362:Organic Anion Transporters, Sodium-Independent / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018528:ATP-Binding Cassette Transporters; C423013:Abcc2 protein, rat; D003287:Contrast Media; D029362:Organic Anion Transporters, Sodium-Independent; C421102:Slco1a1 protein, rat; C073590:gadolinium ethoxybenzyl DTPA; D019786:Gadolinium DTPA