title: A collagen peptide-based physical hydrogel for cell encapsulation.
abstract: Collagen peptide-based hydrogels are prepared and characterized for application in 3D cell growth. Physical hydrogels are formed by covalently linking a collagen-based peptide to an 8-arm poly(ethylene glycol) star polymer. The resulting viscoelastic hydrogels have the ability to melt into a liquid-like state near the melting temperature of the collagen triple helix and reform back into an elastic-state at room temperature, adding a thermoresponsive feature to the material. In addition, the hydrogels possess desirable stiffness, as well as a highly cross-linked network of pores where cells are found to reside, making the hydrogels promising scaffolds for the culture of hMSCs.
authors.affiliation: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA.
authors.forename: Charles M Rubert
authors.initials: CM
authors.lastname: Pérez
authors.forename: Alyssa
authors.initials: A
authors.lastname: Panitch
authors.forename: Jean
authors.initials: J
authors.lastname: Chmielewski
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D018914:Cells, Immobilized; D003094:Collagen / Q000737:chemistry*; D020285:Cryoelectron Microscopy; D055119:Elastic Modulus; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D011489:Protein Denaturation; D012212:Rheology; D013696:Temperature
chemical_list: D020100:Hydrogels; D010455:Peptides; D003094:Collagen