title: Incidence of Phlebiopsis gigantea large virus-1 in a collection of Phlebiopsis gigantea isolates.
abstract: Eighty six Phlebiopsis gigantea isolates from at least 9 different tree species from various locations in 12 different European countries and North America were screened for the presence of large molecular weight dsRNA >10 kbp in size. In 7 isolates, which contained large dsRNAs, the presence of Phlebiopsis gigantea large virus-1 (PgLV-1) was suggested following the sequencing of the RT-PCR amplicons generated with PgLV-1 specific oligonucleotide primers which also revealed little genetic diversity between the virus isolates.
authors.affiliation: Division of Biology, Faculty of Natural Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London, SW7 2AZ, UK.
authors.forename: Jia M
authors.initials: JM
authors.lastname: Lim
authors.forename: Atif
authors.initials: A
authors.lastname: Jamal
authors.forename: Xiyu
authors.initials: X
authors.lastname: Phoon
authors.forename: Kari
authors.initials: K
authors.lastname: Korhonen
authors.forename: Robert H A
authors.initials: RH
authors.lastname: Coutts
mesh_terms: D000595:Amino Acid Sequence; D005060:Europe; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009656:North America; D020072:Polyporales / Q000145:classification / Q000821:virology*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*