title: Comparison of transcriptional and blood cell-phenotypic markers between operationally tolerant liver and kidney recipients.
abstract: A proportion of transplant recipients can spontaneously accept their grafts in the absence of immunosuppression (operational tolerance). Previous studies identified blood transcriptional and cell-phenotypic markers characteristic of either liver or kidney tolerant recipients. However, the small number of patients analyzed and the use of different transcriptional platforms hampered data interpretation. In this study we directly compared samples from kidney and liver tolerant recipients in order to identify potential similarities in immune-related parameters. Liver and kidney tolerant recipients differed in blood expression and B-cell immunophenotypic patterns and no significant overlaps were detectable between them. Whereas some recipients coincided in specific NK-related transcripts, this observation was not reproducible in all cohorts analyzed. Our results reveal that certain immune features, but not others, are consistently present across all cohorts of operationally tolerant recipients. This provides a set of reproducible biomarkers that should be explored in future large-scale immunomonitoring trials.
authors.affiliation: Plataforma de Bioinformatica, CIBEREHD, Hospital Clinic, Barcelona, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lozano
authors.forename: A
authors.initials: A
authors.lastname: Pallier
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Llordella
authors.forename: R
authors.initials: R
authors.lastname: Danger
authors.forename: M
authors.initials: M
authors.lastname: López
authors.forename: M
authors.initials: M
authors.lastname: Giral
authors.forename: M C
authors.initials: MC
authors.lastname: Londoño
authors.forename: A
authors.initials: A
authors.lastname: Rimola
authors.forename: J P
authors.initials: JP
authors.lastname: Soulillou
authors.forename: S
authors.initials: S
authors.lastname: Brouard
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Fueyo
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D016130:Immunophenotyping; D016030:Kidney Transplantation; D016031:Liver Transplantation; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic