title: [Development of a killed but metabolically active anthracis vaccine candidate strain].
abstract: Anthrax is a zoonosis caused by Bacillus anthracis, which seriously affects human health. In recent years, a special phenomenon is found that the metabolic active of a bacterium remains after it is killed. To development of a KBMA (killed but metabolically active) Bacillus anthracis vaccine candidate strain, a plasmid pMAD and a recombinase system Cre-loxP were used to knockout the uvrAB gene of B. anthracis AP422 which lacks both of two plasmids pXO1 and pXO2. The results of PCR and RT-PCR shows that uvrAB genes were deleted from B. anthracis AP422 chromosome successfully. The constructed B. anthracis AP422deltauvrAB was inactivated by photochemical treatment (PCT) including an exposure in a long-wave-length ultraviolet (UVA) light and a treatment of 8-Methoxypsoralen (8-MOP), then the metabolic activity were detected by the method of MTS. The results showed that the killed B. anthracis AP422deltauvrAB maintained a highly metabolic activity for at least 4 hours, showing a state of KBMA. The KBMA strain of B. anthracis AP422deltauvrAB provides the prospective vaccine candidate strain for anthrax.
authors.affiliation: College of Life Sciences, Anhui Normal University, Wuhu 241000, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.forename: Shengling
authors.initials: S
authors.lastname: Yuan
authors.forename: Dewen
authors.initials: D
authors.lastname: Zhan
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Ren
authors.forename: Haoxia
authors.initials: H
authors.lastname: Tao
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Lingchun
authors.initials: L
authors.lastname: Wang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Chen
authors.forename: Chunjie
authors.initials: C
authors.lastname: Liu
mesh_terms: D000881:Anthrax / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D022122:Anthrax Vaccines / Q000235:genetics* / Q000276:immunology* / Q000528:radiation effects; D001408:Bacillus anthracis / Q000235:genetics / Q000276:immunology; D055786:Gene Knockout Techniques; D008730:Methoxsalen / Q000494:pharmacology; D014466:Ultraviolet Rays; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology*
chemical_list: D022122:Anthrax Vaccines; D015164:Vaccines, Inactivated; D008730:Methoxsalen