title: Developing visible fluorogenic 'click-on' dyes for cellular imaging.
abstract: New fluorogenic dyes were designed and synthesized based on Cu(I)-catalyzed 'click' reaction. Conjugating weakly fluorescent benzothiazole derivatives with an electron-deficient alkyne group at the 2-position with azide-containing molecules in aqueous solution form 'click-on' fluorescent adducts. Model reactions and cell culture experiment indicated that the developed 'click-on' dye could be applied to labeling various biomolecules, such as nucleic acids, proteins, and other molecules, in cells.
authors.affiliation: Department of Radiology, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Qi
authors.forename: Myung-Shin
authors.initials: MS
authors.lastname: Han
authors.forename: Yu-Cheng
authors.initials: YC
authors.lastname: Chang
authors.forename: Ching-Hsuan
authors.initials: CH
authors.lastname: Tung
mesh_terms: D001386:Azides / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D001386:Azides; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D003300:Copper; C005465:benzothiazole