title: Characterization of a microbial consortium capable of degrading lignocellulose.
abstract: A microbial consortium, designated WCS-6, was established by successive subcultivation in the presence of rice straw under static conditions. The degradation efficiencies of WSC-6 for 0.5 g filter paper, cotton and rice straw after 3 days of cultivation were 99.0±0.7%, 76.9±1.5% and 81.3±0.8%, respectively as determined by gravimetrical methods. Nine bacterial isolates were obtained from WCS-6 plated under aerobic conditions, and sequencing of their 16S rDNA indicated that these bacteria were related to Bacillus thermoamylovorans BTa, Paenibacillus barengoltzii SAFN-016, Proteobacterium S072, Pseudoxanthomonas taiwanensis CB-226, Rhizobiaceae str. M100, Bacillus sp. E53-10, Beta proteobacterium HMD444, Petrobacter succinimandens 4BON, and Tepidiphilus margaritifer N2-214. DGGE (denaturing gradient gel electrophoresis) and sequencing of 16S rDNA sequences amplified from total consortium DNA revealed the presence of sequences related to those of Ureibacillus thermosphaericus, uncultured bacterium clone GC3, uncultured Clostridium sp. clone A1-3, Clostridium thermobutyricum, and Clostridium thermosuccinogenes in addition to the sequences identified from the cultured bacteria. The microbial community identified herein is a potential candidate consortium for the degradation of waste lignocellulosic biomass.
authors.affiliation: College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, PR China. wwdcyy@126.com
authors.forename: Weidong
authors.initials: W
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Yan
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
authors.forename: Yamei
authors.initials: Y
authors.lastname: Gao
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Jing
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry*; D010802:Phylogeny; D018547:Plant Stems / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; C036909:lignocellulose; D008031:Lignin