title: Identification and characterization of OXA-48 producing, carbapenem-resistant Enterobacteriaceae isolates in Turkey.
abstract: Carbapenem resistance in Enterobacteriaceae isolates has been reported from Turkey and is most often mediated by OXA-48 type carbapenemases. We report the identification and characterization of four carbapenem-resistant isolates (three Klebsiella pneumoniae and one Escherichia coli) among 515 clinical Enterobacteriaceae isolates collected during a 7-month study period in Ankara, Turkey. The four isolates were recovered from blood and urine specimens in patients with varied clinical manifestations. They had distinct pulsed-field gel electrophoresis patterns and harbored a variety of β-lactamases including bla(TEM-1), bla(SHV-12) genes, bla(SHV-11), and/or bla(CTX-M-15). PCR and sequencing analysis revealed that the bla(OXA-48) gene was present in all four isolates. Our data indicated that the OXA-48-type carbapenemase was the only mechanism for carbapenem resistance in our hospital.
authors.affiliation: Departments of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, Ankara, Turkey. abkilic@gata.edu.tr
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kilic
authors.forename: Zerin
authors.initials: Z
authors.lastname: Aktas
authors.forename: Orhan
authors.initials: O
authors.lastname: Bedir
authors.forename: Ramazan
authors.initials: R
authors.lastname: Gumral
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Bulut
authors.forename: Charles
authors.initials: C
authors.lastname: Stratton
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
authors.forename: A Celal
authors.initials: AC
authors.lastname: Basustaoglu
mesh_terms: D000369:Aged, 80 and over; D015780:Carbapenems / Q000494:pharmacology; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D016133:Polymerase Chain Reaction; D014421:Turkey; D055815:Young Adult; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D015780:Carbapenems; D001618:beta-Lactamases; C108449:oxacillinase