title: [Sequence analysis of 16S rDNA gene of endosymbiont of Acanthamoeba sp. CB/S1 isolated from soil].
abstract: The endosymbiont of Acanthamoeba sp. CB/SI was identified by orcein-carmine staining and 16S rDNA sequence analysis. The endosymbiont bacteria were rod-shaped and darkly stained, and irregularly localized within the cytoplasm. The length of the 16S rDNA was 1534 bp and its DNA sequence was closely related to those of Candidatus Amoebophilus asiaticus and Acanthamoeba sp. KA/E21 with 98% homology. Phylogenetic analysis showed that the endosymbiont of CB/SI, the endosymbiont of KA/E21, Candidatus Amoebophilus asiaticus, the endosymbiont of Ixodes scapularis, and the endosymbiont of Encarsia pergandiella constitute a monophyletic lineage in phylogenetic tree.
authors.affiliation: Department of Pathogenic Biology, College of Basic Medicine, Yanbian University, Yanji 133000, China.
authors.forename: Ying-hua
authors.initials: YH
authors.lastname: Xuan
authors.forename: Chun-quan
authors.initials: CQ
authors.lastname: Cui
authors.forename: Shan-zi
authors.initials: SZ
authors.lastname: Zheng
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal