title: A novel single-dose dengue subunit vaccine induces memory immune responses.
abstract: To protect against dengue viral infection, a novel lipidated dengue subunit vaccine was rationally designed to contain the consensus amino acid sequences derived from four serotypes of dengue viruses. We found that the lipidated consensus dengue virus envelope protein domain III (LcED III) is capable of activating antigen-presenting cells and enhancing cellular and humoral immune responses. A single-dose of LcED III immunization in mice without extra adjuvant formulation is sufficient to elicit neutralizing antibodies against all four serotypes of dengue viruses. In addition, strong memory responses were elicited in mice immunized with a single-dose of LcED III. Quick, anamnestic neutralizing antibody responses to a live dengue virus challenge were elicited at week 28 post-immunization. These results demonstrate the promising possibility of a future successful tetravalent vaccine against dengue viral infections that utilizes one-dose vaccination with LcED III.
authors.affiliation: Vaccine Research and Development Center, National Health Research Institutes, Zhunan, Miaoli, Taiwan.
authors.forename: Chen-Yi
authors.initials: CY
authors.lastname: Chiang
authors.forename: Shih-Jen
authors.initials: SJ
authors.lastname: Liu
authors.forename: Jy-Ping
authors.initials: JP
authors.lastname: Tsai
authors.forename: Yi-Shiuan
authors.initials: YS
authors.lastname: Li
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Chen
authors.forename: Hsueh-Hung
authors.initials: HH
authors.lastname: Liu
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
authors.forename: Chih-Hsiang
authors.initials: CH
authors.lastname: Leng
authors.forename: Hsin-Wei
authors.initials: HW
authors.lastname: Chen
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D053059:Dengue Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D020014:K562 Cells; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D053059:Dengue Vaccines