title: Neuroprotective effect of ACE inhibitors in glutamate - induced neurotoxicity: rat neuron culture study.
abstract: OBJECTIVE
  Glutamate is known to be neurotoxic at concentrations of 10-6M and 10-7M. Angiotensin converting enzyme (ACE) inhibitors can be assumed to be neuroprotective as they open the mitochondrial adenosine triphosphate-sensitive potassium channels by inhibiting the degradation of bradykinin. In this study, we investigated whether the ACE inhibitors captopril, ramipril and perindopril have protective effects in glutamate-induced neurotoxicity in newborn rat cerebral cortex cell cultures.
  
  
  METHODS
  Viability tests were performed among ACE inhibitors by constituting groups of control and 10-7M and 10-6M glutamate doses in newborn rat cortex cultures.
  
  
  RESULTS
  While the mean viable cell number was 0.47±0.06 in the control group, it was 0.37±0.03 in the group exposed to 10-7M glutamate (p < 0.05) and 0.37±0.01 in the group exposed to 10-6M glutamate (p < 0.05). Captopril was used at a dose of 10 μM, perindopril was used at a dose of 1 μM, and ramipril was used at a dose of 30 μM against 10-7M and 10-6M glutamate. Ramipril and perindopril reversed the toxicity against 10-6M glutamate (p < 0.05). The neuroprotective properties of captopril, perindopril and ramipril were not found to be statistically significant against 10-7M glutamate at the doses mentioned above.
  
  
  CONCLUSIONS
  Data obtained from this study indicate that ramipril and perindopril can prevent 10-6M glutamate-induced neurotoxicity.
authors.affiliation: Ataturk University, Faculty of Medicine, Department of Neurosurgery, Erzurum, Turkey. goksinsengul@gmail.com
authors.forename: Goksin
authors.initials: G
authors.lastname: Sengul
authors.forename: Suleyman
authors.initials: S
authors.lastname: Coskun
authors.forename: Murteza
authors.initials: M
authors.lastname: Cakir
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Coban
authors.forename: Fatih
authors.initials: F
authors.lastname: Saruhan
authors.forename: Ahmet
authors.initials: A
authors.lastname: Hacimuftuoglu
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002216:Captopril / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D020913:Perindopril / Q000494:pharmacology; D017257:Ramipril / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D018696:Neuroprotective Agents; D018698:Glutamic Acid; D002216:Captopril; D017257:Ramipril; D020913:Perindopril