title: Simultaneous quantitative determination of 9 active components in traditional Chinese medicinal preparation ShuangDan oral liquid by RP-HPLC coupled with photodiode array detection.
abstract: A simple, accurate and reliable method for the simultaneous separation and determination of 9 active components (danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, salvianolic acid B, paeonol, paeoniflorin and gallic acid) in traditional Chinese medicinal preparation ShuangDan (SD) oral liquid was developed using reverse phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array (PDA) detection. The chromatographic separation was performed on a SinoChrom ODS-BP C(18) column with gradient elution using methanol (A) and 3% glacial acetic acid aqueous solution (B) at a flow rate of 1.0mLmin(-1), and with a PDA detection. Good linear behaviors over the investigated concentration ranges were observed with the values of r(2) higher than 0.9992 for all the analytes. The recoveries and relative standard deviation (RSD), measured at three concentration levels, varied from 98.21% to 101.82% and 0.07% to 1.37%, respectively. The proposed method enables the simultaneous identification and determination of 9 active components in a single run for the quality control of ShuangDan oral liquid.
authors.affiliation: Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, Xi'an 710032, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Si-Wang
authors.initials: SW
authors.lastname: Wang
authors.forename: Bang-Le
authors.initials: BL
authors.lastname: Zhang
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Xie
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D002109:Caffeic Acids / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D056148:Chromatography, Reverse-Phase; D003327:Coronary Disease / Q000188:drug therapy; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000627:therapeutic use; D062385:Hydroxybenzoates / Q000032:analysis* / Q000737:chemistry; D028321:Plant Preparations / Q000032:analysis* / Q000737:chemistry / Q000627:therapeutic use; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D028321:Plant Preparations; C521472:Shuangdan; C009091:protocatechuic acid; C040048:caffeic acid