title: Electrostatic braiding and homologous pairing of DNA double helices.
abstract: Homologous pairing and braiding (supercoiling) have crucial effects on genome organization, maintenance, and evolution. Generally, the pairing and braiding processes are discussed in different contexts, independently of each other. However, analysis of electrostatic interactions between DNA double helices suggests that in some situations these processes may be related. Here we present a theory of DNA braiding that accounts for the elastic energy of DNA double helices as well as for the chiral nature of the discrete helical patterns of DNA charges. This theory shows that DNA braiding may be affected, stabilized, or even driven by chiral electrostatic interactions. For example, electrostatically driven braiding may explain the surprising recent observation of stable pairing of homologous double-stranded DNA in solutions containing only monovalent salt. Electrostatic stabilization of left-handed braids may stand behind the chiral selectivity of type II topoisomerases and positive plasmid supercoiling in hyperthermophilic bacteria and archea.
authors.affiliation: Department of Chemistry, Imperial College London, London, UK. ruggero.cortini@gmail.com
authors.forename: Ruggero
authors.initials: R
authors.lastname: Cortini
authors.forename: Alexei A
authors.initials: AA
authors.lastname: Kornyshev
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Lee
authors.forename: Sergey
authors.initials: S
authors.lastname: Leikin
mesh_terms: D020029:Base Pairing / Q000187:drug effects; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004573:Electrolytes / Q000494:pharmacology; D059765:Homologous Recombination / Q000187:drug effects; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009994:Osmolar Concentration; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D055672:Static Electricity; D013816:Thermodynamics
chemical_list: D004573:Electrolytes; D012965:Sodium Chloride; D004247:DNA