title: Proteomic reactors and their applications in biology.
abstract: Proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow HPLC, MS and bioinformatics. Although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. Along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. These microfluidic devices provide rapid, robust and efficient pre-MS sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. The proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade.
authors.affiliation: Ottawa Institute of Systems Biology (OISB), University of Ottawa, Ottawa, ON, Canada.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Ning
authors.forename: Fangjun
authors.initials: F
authors.lastname: Wang
authors.forename: Deeptee
authors.initials: D
authors.lastname: Seebun
authors.forename: Daniel
authors.initials: D
authors.lastname: Figeys
mesh_terms: D000818:Animals; D019149:Bioreactors; D004798:Enzymes / Q000378:metabolism; D006801:Humans; D046210:Microfluidic Analytical Techniques; D040901:Proteomics
chemical_list: D004798:Enzymes