title: Lentivirus-mediated modification of pluripotent stem cells.
abstract: Relatively safe, HIV-1-based lentiviral vectors have served as an efficient means of transducing human embryonic stem cells (hESCs). Here we describe the variety of lentiviral vector systems available with the basic strategy for designing viral vectors and methods for generating viruses for efficiently infecting and selecting transduced hESCs.
authors.affiliation: Wysocka lab, Department of Chemical and Systems Biology, School of Medicine, Stanford University, Palo Alto, CA, USA. rbajpai@stanford.edu
authors.forename: Ruchi
authors.initials: R
authors.lastname: Bajpai
mesh_terms: D002478:Cells, Cultured; D002999:Clone Cells; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D009355:Neomycin / Q000494:pharmacology; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000821:virology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014161:Transduction, Genetic / Q000379:methods*; D014162:Transfection; D019076:Transgenes / Q000235:genetics; D019065:Virus Assembly / Q000187:drug effects
chemical_list: D049452:Green Fluorescent Proteins; D009355:Neomycin