title: Cyst formation, increased anti-inflammatory cytokines and expression of chemokines support for Clonorchis sinensis infection in FVB mice.
abstract: To verify the hypothesis that different pathology of Clonorchis sinensis infection by mouse strains is determined by different responses of cytokines and chemokines, we compared those responses of FVB with those of BALB/c mice. All of FVB mice infected with 30 metacercariae of C. sinensis showed cystic dilatation in the liver, whereas infected BALB/c mice did not. Mature worms were recovered from 19 of 20 liver sections of FVB mice while only one of 20 sections of BALB/c mice revealed a mature worm. In both strains the proportion of CD4(+) T cells was lower in C. sinensis-infected than in the uninfected group. However, the proportion of CD8(+) T cells was elevated in C. sinensis-infected from both strains compared to uninfected mice. The Th2-associated anti-inflammatory cytokines such as IL-4, IL-5 and IL-13, IL-10 and TGF-β, were significantly more produced by the lymphocytes of FVB than by those of BALB/c mice. Especially, the 2 anti-inflammatory cytokines, IL-10 and TGF-β, were presumably related with susceptibility and the development of worms in the liver. C. sinensis infected FVB mice also produced more chemokines such as RANTES and MIP-1α in the liver lymphocytes than BALB/c mice. In conclusion, the FVB mice provide the favorable niche for C. sinensis by cyst formation in the bile duct, increased production of Th2-associated anti-inflammatory cytokines and upregulation of chemokines.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea.
authors.forename: Eun-Min
authors.initials: EM
authors.lastname: Kim
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Bae
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Choi
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Hong
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000378:metabolism; D018925:Chemokines / Q000276:immunology*; D003003:Clonorchiasis / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D003004:Clonorchis sinensis / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D003560:Cysts / Q000469:parasitology / Q000473:pathology*; D016207:Cytokines / Q000276:immunology*; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D008099:Liver / Q000469:parasitology / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D013045:Species Specificity
chemical_list: D000909:Antibodies, Helminth; D018925:Chemokines; D016207:Cytokines