title: Gene expression profiling of Atlantic cod (Gadus morhua) embryogenesis using microarray.
abstract: Atlantic cod (Gadus morhua) is a fish species of high importance, as a key species in a range of Northern ecosystems, in fisheries, and as an emerging species in aquaculture. So far, little is known about the transcriptional activity during early developmental stages of Atlantic cod. Hence, we decided to use a cDNA microarray covering 7,000 genes to analyze the temporal activity of the transcriptome during cod embryogenesis. Twelve different embryonic time points were selected, covering major developmental stages and processes such as maternally derived mRNA, blastula, gastrula, segmentation, hatching, and first-feeding larval stage. The microarray analysis revealed a highly dynamic transcriptional profile, showing for the first time the differential expression of thousands of known and unknown genes during Atlantic cod embryogenesis. These initial findings will serve as an important baseline for future in-depth studies of candidate genes involved in development, reproductive control, disease resistance, growth, nutrient digestion, and metabolism.
authors.affiliation: Institute of Marine Research, P.O. Box 1870, Nordnes, 5817 Bergen, Norway.
authors.forename: Øyvind
authors.initials: Ø
authors.lastname: Drivenes
authors.forename: Geir Lasse
authors.initials: GL
authors.lastname: Taranger
authors.forename: Rolf B
authors.initials: RB
authors.lastname: Edvardsen
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D048389:Gadus morhua / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods