title: Two-photon Lysotrackers for in vivo imaging.
abstract: We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750-840 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time through two-photon microscopy. When combined with appropriate two-photon probes for other biological targets, these novel probes would greatly facilitate the two-photon microscopy colocalization experiments.
authors.affiliation: Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Son
authors.forename: Chang Su
authors.initials: CS
authors.lastname: Lim
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Han
authors.forename: Isravel Antony
authors.initials: IA
authors.lastname: Danish
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D000588:Amines / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*; D006624:Hippocampus / Q000166:cytology; D057054:Molecular Imaging / Q000379:methods*; D017785:Photons; D051381:Rats
chemical_list: D000588:Amines; D005456:Fluorescent Dyes; C493330:Red DND-99