title: A near-infrared fluorescent calcium probe: a new tool for intracellular multicolour Ca2+ imaging.
abstract: We report a novel near-infrared fluorescent calcium probe (KFCA), which has good optical properties such as intense NIR fluorescence emission (670 nm, QY: 0.24), excellent ON/OFF ratio (120-fold), and good wavelength-compatibility with visible-light-emissive fluorophores (Fluo-4, DsRed2), and which is applicable for real-time dual-colour intracellular Ca(2+) imaging.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Yokohama 223-8522, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Matsui
authors.forename: Keitaro
authors.initials: K
authors.lastname: Umezawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shindo
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Fujii
authors.forename: Daniel
authors.initials: D
authors.lastname: Citterio
authors.forename: Kotaro
authors.initials: K
authors.lastname: Oka
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D001896:Boron Compounds / Q000737:chemistry; D002118:Calcium / Q000378:metabolism*; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007259:Infrared Rays; D042541:Intracellular Space / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D055620:Optical Phenomena
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D002118:Calcium