title: Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis.
abstract: Transforming growth factor (TGF)-β and bone morphogenetic protein (BMP) induce a cartilage-specific extracellular matrix (ECM) gene, aggrecan, in a chondrogenic cell line, ATDC5. The results of our recent study show that TGF-β1, but not BMP-4, strongly induces an ECM gene, fibronectin, during chondrogenesis. However, the role of fibronectin in chondrogenesis is unclear. In the current study, our results showed that TGF-β1, but not BMP-4, led to versican-dominant proteoglycan production during chondrogenesis of ATDC5 cells. siRNA-mediated reduction of fibronectin and interference in the liaison between fibronectin and integrins by the Arg-Gly-Asp-Ser (RGDS) peptide increased aggrecan expression, and decreased versican expression by TGF-β1 stimulation. These data suggest that fibronectin is a critical mediator for TGF-β-specific production balance of 2 major proteoglycans, aggrecan and versican, during chondrogenesis.
authors.affiliation: Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Shitsukawa, Toon, Ehime, Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kutsuna
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Inoue
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Takeda
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takahashi
authors.forename: Haruyasu
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Miura
authors.forename: Shigeki
authors.initials: S
authors.lastname: Higashiyama
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D020219:Chondrogenesis / Q000187:drug effects* / Q000235:genetics; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D008954:Models, Biological; D011509:Proteoglycans / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D011509:Proteoglycans; D053773:Transforming Growth Factor beta1