title: Genotoxic evaluation of a methanolic extract of Verbascum thapsus using micronucleus test in mouse bone marrow.
abstract: Verbascum thapsus L. is a medicinal plant and has been used to treat numerous pulmonary diseases, asthma, inflammatory disease, spasmodic coughs and migraine headaches. Several studies have demonstrated that different extracts of V. thapsus present antimicrobial activity. Thus, the goal of this study was to evaluate the genotoxic and cytotoxic activities of a methanolic extract of Verbascum thapsus, using micronucleus test in mouse bone marrow. No toxicity in bone marrow was detected in the extract-treated groups. The methanolic extract of V. thapsus at doses of 100, 300 and 500 mg/kg, did not produce a significant increase in the frequency of MNPCE in bone marrow and neither altered the relationship PCE/NCE respect to negative control. These cytogenotoxic findings contribute the preclinical knowledge of methanolic extract of V. thapsus and provide security in its use as herbal medicine.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. fescobar@exa.unrc.edu.ar
authors.forename: Franco Matías
authors.initials: FM
authors.lastname: Escobar
authors.forename: María Carola
authors.initials: MC
authors.lastname: Sabini
authors.forename: Silvia Matilde
authors.initials: SM
authors.lastname: Zanon
authors.forename: Laura Noelia
authors.initials: LN
authors.lastname: Cariddi
authors.forename: Carlos Eugenio
authors.initials: CE
authors.lastname: Tonn
authors.forename: Liliana Inés
authors.initials: LI
authors.lastname: Sabini
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D032265:Verbascum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts