title: Enhanced oxidative stress and platelet activation combined with reduced antioxidant capacity in obese prepubertal and adolescent girls with full or partial metabolic syndrome.
abstract: In adults, obesity is a main factor implicated in increased oxidative stress (OS), platelet activation (PA) and impaired antioxidant status (AS), all predisposing factors for cardiovascular disease leading to increased morbidity and mortality. Furthermore, the metabolic syndrome (MetS) is an important cardiovascular risk factor, which progressively develops and may already be present during late childhood or adolescence. However, scarce data exist on oxidative-antioxidant balance and PA in childhood and adolescence in the presence of partial (PMetS) or full MetS. The aim of the study was to evaluate OS, PA, and AS in prepubertal and adolescent obese girls with partial or full MetS. 96 girls with a clinical and metabolic evaluation for obesity and 44 healthy normal-weight sex- and age-matched girls were studied. IDF-adopted criteria were used to define full and partial MetS and the patient population was divided into 4 groups: the first comprised 31 pre-pubertal girls with PMetS (PR-PMetS), the second 37 adolescents with PMetS (AD-PMetS), the third 10 prepubertal girls with full MetS (PR-MetS), and the fourth 18 adolescents with full MetS (AD-MetS). The OS was evaluated by measuring plasma 15-F(2t)-Isoprostane levels (15-F(2t)-IsoP) and protein carbonyls, PA by thromboxane B(2) levels (TXB(2)), and AS by serum vitamin E and plasma total antioxidant capacity (TAC) levels. 15-F(2t)-IsoP, protein carbonyls, and TXB(2) levels were significantly gradually amplified, and vitamin E and TAC reduced, and significantly correlated with obesity from childhood to adolescence and from partial to full MetS. This study demonstrates the loss of the normal homeostatic balance between oxidant-antioxidant state in obese children and adolescents with manifestations of partial and full MetS.
authors.affiliation: Division of Endocrinology, Diabetology and Metabolism, First Department of Pediatrics, Athens University Medical School, Aghia Sophia Children's Hospital, Athens, Greece.
authors.forename: I
authors.initials: I
authors.lastname: Karamouzis
authors.forename: P
authors.initials: P
authors.lastname: Pervanidou
authors.forename: R
authors.initials: R
authors.lastname: Berardelli
authors.forename: S
authors.initials: S
authors.lastname: Iliadis
authors.forename: I
authors.initials: I
authors.lastname: Papassotiriou
authors.forename: M
authors.initials: M
authors.lastname: Karamouzis
authors.forename: G P
authors.initials: GP
authors.lastname: Chrousos
authors.forename: C
authors.initials: C
authors.lastname: Kanaka-Gantenbein
mesh_terms: D000293:Adolescent; D041923:Adolescent Development; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D002648:Child; D015536:Down-Regulation; D006801:Humans; D024821:Metabolic Syndrome / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D009765:Obesity / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D018384:Oxidative Stress; D015539:Platelet Activation; D011627:Puberty; D015854:Up-Regulation
chemical_list: D000975:Antioxidants