title: ECM-based triple layered scaffolds for vascular tissue engineering.
abstract: The present study focused on the development of three layered small-diameter (<6 mm) extracellular matrix (ECM)-based vessels. These were engineered artificially through the freeze-drying technique. A layer of decellularized bovine aorta (DAM) was deposited on a mandrel and, after lyophilization, it was dipped into a poly-L-lactide acid (PLLA)/polyethylene glycol (PEG) 2000 dichloromethane solution then quickly wrapped with a pre-prepared thin DAM sheet. Mechanical properties of three-layered scaffolds were evaluated by means of uniaxial tensile measurement. Furthermore, human endothelial and smooth muscle cells were seeded on internal and external scaffold surfaces, respectively, and co-cultured for 7 days. Our results demonstrate that i) ECM components provide suitable stimuli for cell adhesion and proliferation, ii) the microporous intermediate PLLA/PEG2000 layer is responsible for the scaffold resistance and iii) the layered deposition technique can be considered a valuable method to obtain layered vascular scaffolds of different sizes and with a good compromise between stiffness and elasticity for optimal cell organization.
authors.affiliation: Department of Pharmaceutical Sciences, University of Padua, Via Marzolo 5, I-35131 Padua, Italy. claudio.grandi@unipd.it
authors.forename: Claudio
authors.initials: C
authors.lastname: Grandi
authors.forename: Francesca
authors.initials: F
authors.lastname: Martorina
authors.forename: Silvano
authors.initials: S
authors.lastname: Lora
authors.forename: Daniele
authors.initials: D
authors.lastname: Dalzoppo
authors.forename: Pietro
authors.initials: P
authors.lastname: Amistà
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sartore
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Liddo
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
mesh_terms: D000818:Animals; D001011:Aorta / Q000033:anatomy & histology / Q000737:chemistry* / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D004548:Elasticity; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005612:Freeze Drying; D006801:Humans; D008422:Materials Testing; D008752:Methylene Chloride / Q000737:chemistry; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011092:Polyethylene Glycols; C033616:poly(lactide); D008752:Methylene Chloride