title: GATA5 interacts with GATA4 and GATA6 in outflow tract development.
abstract: Members of the GATA family of transcription factors are critical regulators of heart development and mutations in 2 of them, GATA4 and GATA6 are associated with outflow tract and septal defects in human. The heart expresses 3 GATA factors, GATA4, 5 and 6 in a partially overlapping pattern. Here, we report that compound Gata4/Gata5 and Gata5/Gata6 mutants die embryonically or perinatally due to severe congenital heart defects. Almost all Gata4(+/-)Gata5(+/-) mutant embryos have double outlet right ventricles (DORV), large ventricular septal defects (VSD) as well as hypertrophied mitral and tricuspid valves. Only 25% of double compound Gata4/Gata5 heterozygotes survive to adulthood and these mice have aortic stenosis. Compound loss of a Gata5 and a Gata6 allele also leads to DORVs associated with subaortic VSDs. Expression of several transcription factors important for endocardial and myocardial cell differentiation, such as Tbx20, Mef2c, Hey1 and Hand2, was reduced in compound heterozygote embryos. These findings suggest the existence of important genetic interactions between Gata5 and the 2 other cardiac GATA factors in endocardial cushion formation and outflow tract morphogenesis. The data identify GATA5 as a potential genetic modifier of congenital heart disease and provide insight for elucidating the genetic basis of an important class of human birth defects.
authors.affiliation: Laboratoire de Développement et Différentiation Cardiaque, Programme de Biologie Moléculaire, Université de Montréal, Montréal QC, Canada H3C 3J7.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Laforest
authors.forename: Mona
authors.initials: M
authors.lastname: Nemer
mesh_terms: D000818:Animals; D001024:Aortic Valve Stenosis / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D004694:Endocardial Cushion Defects / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D005260:Female; D005318:Fetal Heart / Q000196:embryology* / Q000378:metabolism*; D050991:GATA4 Transcription Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D050992:GATA5 Transcription Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D050993:GATA6 Transcription Factor / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006330:Heart Defects, Congenital / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D006345:Heart Septal Defects, Ventricular / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D006579:Heterozygote; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D050991:GATA4 Transcription Factor; D050992:GATA5 Transcription Factor; D050993:GATA6 Transcription Factor; C484171:Gata4 protein, mouse; C494756:Gata5 protein, mouse; C494760:Gata6 protein, mouse; D012333:RNA, Messenger