title: Effect of genetic polymorphism of GSTM1 and GSTT1 genotypes on cytogenetic biomarkers among coaltar workers.
abstract: Chromosomal aberrations (CAs) in peripheral blood lymphocytes and micronuclei (MN) in exfoliated buccal cells have been used for decades as cytogenetic biomarkers to investigate genotoxicity among occupationally or environmentally exposed population. In our study, we investigated the association of increased cytogenetic damage with genetic polymorphism in glutathione-S transferase genotypes among occupationally exposed 115 coaltar workers and 105 unexposed controls. We found higher mean value of chromosome aberrations (chromatid type-2.01±1.76; chromosomal type-2.22±1.73) and buccal micronuclei (BMN-7.10±1.56) in exposed subjects when compared to referents (chromatid type-0.82±.51; chromosomal type-0.87±.54; BMN-5.09±2.88). We observed that individuals having null genotype of GSTM1 and GSTT1 have significantly higher frequency of CAs and MN. Despite of small sample size, our findings suggest a significant association between polymorphism of glutathione-S transferase genotypes and cytogenetic biomarkers which are considered as early effects of genotoxic carcinogens.
authors.affiliation: Department of Biotechnology, Kurukshetra University Kurukshetra, Haryana, India.
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.forename: Shiv Kumar
authors.initials: SK
authors.lastname: Giri
authors.forename: Kapil
authors.initials: K
authors.lastname: Dev
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Gautam
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: Neeraj
authors.initials: N
authors.lastname: Aggarwal
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D002273:Carcinogens / Q000633:toxicity; D002869:Chromosome Aberrations / Q000139:chemically induced; D003033:Coal Tar / Q000633:toxicity*; D003582:Cytogenetics / Q000379:methods*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007641:Keratolytic Agents / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008297:Male; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000187:drug effects / Q000502:physiology; D016273:Occupational Exposure; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002273:Carcinogens; D003033:Coal Tar; D005982:Glutathione Transferase; D007641:Keratolytic Agents; C413545:glutathione S-transferase T1