title: RANTES gene mRNA expression and its -403 G/A promoter polymorphism in coronary artery disease.
abstract: OBJECTIVE
  Increased RANTES expression has been described to have a role in atherosclerosis plaque formation. Functional polymorphisms within RANTES promoter region have shown association with increased risk of coronary atherosclerosis (CAD). The aim of this study was to examine the RANTES mRNA expression in patients with CAD compared to patients without CAD and its association with RANTES -403 G/A polymorphism in an Iranian population.
  
  
  METHODS
  The study was performed on 319 patients who underwent coronary artery angiography and patients with >50% stenosis in vessels considered as case groups (CAD+) N=191 and normal vessels group as control (CAD-) N=128. In each group 20 patients were examined for RANTES mRNA expression. RANTES mRNA expression was examined using quantitative real-time PCR. Genotyping of -403 polymorphism was performed using PCR-RFLP technique.
  
  
  RESULTS
  We found that RANTES mRNA expression was increased to 1.37 fold in CAD patients compared to the controls but the difference was not statistically significant. Also comparing the RANTES mRNA expression in patients with different RANTES -403 G/A polymorphism showed that in patients carrying AA genotype RANTES mRNA expression was increased to 1.74 fold compared to patients carrying GG genotype and to 1.51 fold compared to patients carrying GA genotype. No significant difference for allele and genotype frequencies of RANTES -403 polymorphism was found between cases and controls.
  
  
  CONCLUSIONS
  More studies on larger number of samples are required to further evaluate role of RANTES in pathogenesis of CAD.
authors.affiliation: Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Tavakkoly-Bazzaz
authors.forename: Parvin
authors.initials: P
authors.lastname: Amiri
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tajmir-Riahi
authors.forename: Daryoosh
authors.initials: D
authors.lastname: Javidi
authors.forename: Maryam
authors.initials: M
authors.lastname: Khojasteh-Fard
authors.forename: Zahra
authors.initials: Z
authors.lastname: Taheri
authors.forename: Aida
authors.initials: A
authors.lastname: Tabrizi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Keramatipour
authors.forename: Mahsa M
authors.initials: MM
authors.lastname: Amoli
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D018946:Chemokine CCL5 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018946:Chemokine CCL5; D012333:RNA, Messenger