title: [Neuregulin promotes spermatogonia proliferation in mice].
abstract: OBJECTIVE
  To explore the role of neuregulin (neural regulation of protein, NRG) in the process of mouse spermatogonia proliferation.
  
  
  METHODS
  Mouse testis fragments were cultured in the medium DMEM containing purified NRG1beta or NRG3 at the concentration of 50, 100 and 200 ng/ml, respectively, followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.
  
  
  RESULTS
  Compared with the control group, neuregulin significantly promoted the proliferation of spermatogonia (P < 0.05). The proliferation rates of spermatogonia cultured in the medium with 50, 100 and 200 ng/ml of NRG13 were 1.69, 1.55 and 1.86 times, and those with 50, 100 and 200 ng/ml of NRG3 were 1.35, 1.54 and 2.11 times that of the control.
  
  
  CONCLUSIONS
  NRG1beta and NRG3 can promote the proliferation of mouse spermatogonia, and NRG is expected to be applied in the treatment of male infertility.
authors.affiliation: Department of Dermatology, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, China.
authors.forename: Ying-ji
authors.initials: YJ
authors.lastname: Jin
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Pan
authors.forename: Yu-ji
authors.initials: YJ
authors.lastname: Jin
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020890:Neuregulin-1 / Q000494:pharmacology*; D020880:Neuregulins; D015398:Signal Transduction; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D020890:Neuregulin-1; D020880:Neuregulins; C490581:Nrg1 protein, mouse; C108113:Nrg3 protein, mouse