title: Association of GSTM1 and GSTT1 polymorphisms with DNA damage in coal-tar workers.
abstract: DNA damage was evaluated by alkaline comet assay in peripheral blood lymphocytes of 115 coal-tar workers occupationally exposed to polycyclic aromatic hydrocarbons (PAHs) and 105 control subjects. The effect of polymorphisms of glutathione S-transferase (GST) genotypes on the DNA damage was assessed. The mean tail moment (TM) value in the coal-tar workers was significantly higher as compared to the control subjects (12.06 ± 0.55 versus 0.44 ± 0.31; P<0.05). No significant association (P>0.05) between the GSTT1 and GSTM1 genotypes and the TM values was found, however highest mean rank TM value was reported in GSTM1 null and GSTT1 null genotypes in both control and exposed subjects. Our results suggest that there is increased DNA damage in coal-tar workers due to PAHs exposure. Polymorphisms in GSTM1 and GSTT1 genes do not show significant effect (P>0.05) on DNA damage.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra - 136119 (Haryana), India.
authors.forename: Shiv Kumar
authors.initials: SK
authors.lastname: Giri
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Kapil
authors.initials: K
authors.lastname: Dev
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: Neeraj
authors.initials: N
authors.lastname: Aggarwal
authors.forename: Neena
authors.initials: N
authors.lastname: Seth
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Gautam
mesh_terms: D000328:Adult; D003033:Coal Tar; D020552:Comet Assay; D004249:DNA Damage; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D011110:Polymorphism, Genetic; D017132:Workplace / Q000592:standards; D055815:Young Adult
chemical_list: D003033:Coal Tar; D005982:Glutathione Transferase; D011084:Polycyclic Aromatic Hydrocarbons; C413545:glutathione S-transferase T1