title: Fatty acid binding protein 1 is related with development of aspirin-exacerbated respiratory disease.
abstract: BACKGROUND
  Aspirin-exacerbated respiratory disease (AERD) refers to the development of bronchoconstriction in asthmatics following the ingestion of aspirin. Although alterations in eicosanoid metabolites play a role in AERD, other immune or inflammatory mechanisms may be involved. We aimed to identify proteins that were differentially expressed in nasal polyps between patients with AERD and aspirin-tolerant asthma (ATA).
  
  
  RESULTS
  Two-dimensional electrophoresis was adopted for differential display proteomics. Proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS). Western blotting and immunohistochemical staining were performed to compare the amount of fatty acid-binding protein 1 (FABP1) in the nasal polyps of patients with AERD and ATA. Fifteen proteins were significantly up- (seven spots) or down-regulated in the nasal polyps of patients with AERD (n = 5) compared to those with ATA (n = 8). LC-MS revealed an increase in seven proteins expression and a decrease in eight proteins expression in patients with AERD compared to those with ATA (P = 0.003-0.045). FABP1-expression based on immunoblotting and immunohistochemical analysis was significantly higher in the nasal polyps of patients with AERD compared to that in patients with ATA. FABP1 was observed in epithelial, eosinophils, macrophages, and the smooth-muscle cells of blood vessels in the polyps.
  
  
  CONCLUSIONS
  Our results indicate that alterations in 15 proteins, including FABP1, may be related to the development of AERD.
authors.affiliation: Genome Research Center for Allergy and Respiratory Disease, Soonchunhyang University Bucheon Hospital, 1174, Jung-Dong, Wonmi-Gu, Bucheon, Gyeonggi-Do, South Korea.
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Kim
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Lee
authors.forename: Jong-Sook
authors.initials: JS
authors.lastname: Park
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Park
authors.forename: An-Soo
authors.initials: AS
authors.lastname: Jang
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
authors.forename: Jang-Yul
authors.initials: JY
authors.lastname: Byun
authors.forename: Soo-Taek
authors.initials: ST
authors.lastname: Uh
authors.forename: Eun-Suk
authors.initials: ES
authors.lastname: Koh
authors.forename: Il Yup
authors.initials: IY
authors.lastname: Chung
authors.forename: Choon-Sik
authors.initials: CS
authors.lastname: Park
mesh_terms: D001241:Aspirin / Q000009:adverse effects*; D015153:Blotting, Western; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D050556:Fatty Acid-Binding Proteins / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D012140:Respiratory Tract Diseases / Q000139:chemically induced* / Q000503:physiopathology; D053719:Tandem Mass Spectrometry
chemical_list: D050556:Fatty Acid-Binding Proteins; D001241:Aspirin