title: Suppression of jasmonic acid-dependent defense in cotton plant by the mealybug Phenacoccus solenopsis.
abstract: The solenopsis mealybug, Phenacoccus solenopsis, has been recently recognized as an aggressively invasive pest in China, and is now becoming a serious threat to the cotton industry in the country. Thus, it is necessary to investigate the molecular mechanisms employed by cotton for defending against P. solenopsis before the pest populations reach epidemic levels. Here, we examined the effects of exogenous jasmonic acid (JA), salicylic acid (SA), and herbivory treatments on feeding behavior and on development of female P. solenopsis. Further, we compared the volatile emissions of cotton plants upon JA, SA, and herbivory treatments, as well as the time-related changes in gossypol production and defense-related genes. Female adult P. solenopsis were repelled by leaves from JA-treated plant, but were not repelled by leaves from SA-treated plants. In contrast, females were attracted by leaves from plants pre-infested by P. solenopsis. The diverse feeding responses by P. solenopsis were due to the difference in volatile emission of plants from different treatments. Furthermore, we show that JA-treated plants slowed P. solenopsis development, but plants pre-infested by P. solenopsis accelerated its development. We also show that P. solenopsis feeding inhibited the JA-regulated gossypol production, and prevented the induction of JA-related genes. We conclude that P. solenopsis is able to prevent the activation of JA-dependent defenses associated with basal resistance to mealybugs.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhu
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jinming
authors.initials: J
authors.lastname: Zhang
authors.forename: Yaobin
authors.initials: Y
authors.lastname: Lu
authors.forename: Yongming
authors.initials: Y
authors.lastname: Ruan
mesh_terms: D000818:Animals; D002755:Choice Behavior / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology*; D005260:Female; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D003368:Gossypium / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000469:parasitology*; D006072:Gossypol / Q000032:analysis; D006430:Hemiptera / Q000187:drug effects / Q000502:physiology*; D060434:Herbivory / Q000187:drug effects; D054883:Oxylipins / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects; D025341:Principal Component Analysis; D020156:Salicylic Acid / Q000494:pharmacology; D013997:Time Factors; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D055549:Volatile Organic Compounds; C011006:jasmonic acid; D006072:Gossypol; D020156:Salicylic Acid