title: [Effect of hypoxia on activation of the peripheral blood T lymphocyte in rats].
abstract: OBJECTIVE
  To explore the effect of hypoxia on the peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats so as to provide the basis for studying the intervention measure.
  
  
  METHODS
  Before hypoxia and during hypoxia at 8 000 m for 8 h, 3 d, 6 d and 10 d the change of peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats were detected by flowcytometer with three-color immunofluorescence label.
  
  
  RESULTS
  Rats were exposed to hypoxia at 8 000 m for 8 hours, and CD3+, CD8+, CD8+ CD28- lymphocyte percentages were significantly decreased (P < 0.01) compared with that before hypoxia. After 3 days of hypoxia, besides aforesaid change, CD4+ CD28+ lymphocyte percentage also prominently decreased (P < 0.01) and CD4+ CD28- prominently increased (P < 0.01). After 6 and 10 days of hypoxia, CD3+, CD4+ lymphocyte percentages were further decreased, while CD8+ CD28+ lymphocyte percentage significantly increased (P < 0.01).
  
  
  CONCLUSIONS
  After exposed to hypoxia at 8 000 m for 8 hours and 3 days, activation of CD8+ and CD4+ T lymphocyte was prominently decreased, while with the prolong of exposed time activation of CD8+ T lymphocyte was significantly increased.
authors.affiliation: Environmental Medicine Center, Institute of Health and Environmental Medicine Academy of Military Medical Sciences, Tianjin 300050, China.
authors.forename: Yun-Mei
authors.initials: YM
authors.lastname: Tian
authors.forename: Hong-Jing
authors.initials: HJ
authors.lastname: Nie
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Liu
authors.forename: Yan-Kun
authors.initials: YK
authors.lastname: Zhang
authors.forename: Dong-Xiang
authors.initials: DX
authors.lastname: Zhang
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000531:Altitude; D000532:Altitude Sickness / Q000503:physiopathology; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D000860:Hypoxia / Q000276:immunology / Q000503:physiopathology*; D008213:Lymphocyte Activation / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000502:physiology*