title: Hypoxia stimulates the production of the angiogenesis inhibitor 2-methoxyestradiol by swine granulosa cells.
abstract: We previously demonstrated the presence of 2-methoxyestradiol (2-ME) in swine follicular fluid. Present study was aimed first of all to investigate if swine granulosa cell produce 2-ME; in addition, we tried to assess a potential effect of hypoxia in modulating 2-ME output. Finally, we explored the effect of 2-ME in an angiogenesis bioassay set up in our lab. Our data show that cultured granulosa cells are able to produce 2-ME; interestingly, the secretion of the hormone appeared to be stimulated by hypoxia. Angiogenesis bioassay points out that 2-ME displays an inhibitory effect on neovascularisation. Therefore our data suggest that 2-ME could be a local effector in determining the fine tuning responsible for follicle angiogenesis. These data deserve special attention since the ovary is a valuable experimental model in angiogenesis research.
authors.affiliation: Dipartimento di Produzioni Animali, Biotecnologie Veterinarie, Qualità e Sicurezza degli Alimenti, Sezione di Fisiologia Veterinaria, Università degli Studi di Parma, Via del Taglio 8, 43126 Parma, Italy. basini@unipr.it
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Basini
authors.forename: Francesca
authors.initials: F
authors.lastname: Grasselli
authors.forename: Simona
authors.initials: S
authors.lastname: Bussolati
authors.forename: Laura
authors.initials: L
authors.lastname: Baioni
authors.forename: Federica
authors.initials: F
authors.lastname: Bianchi
authors.forename: Marilena
authors.initials: M
authors.lastname: Musci
authors.forename: Maria
authors.initials: M
authors.lastname: Careri
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mangia
mesh_terms: D000077584:2-Methoxyestradiol; D020533:Angiogenesis Inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000166:cytology; D001681:Biological Assay; D015687:Cell Hypoxia; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000494:pharmacology; D005260:Female; D005337:Fibrin / Q000378:metabolism; D006107:Granulosa Cells / Q000378:metabolism*; D015203:Reproducibility of Results; D013552:Swine
chemical_list: D020533:Angiogenesis Inhibitors; D003470:Culture Media; D004958:Estradiol; D000077584:2-Methoxyestradiol; D005337:Fibrin