title: Fabrication of mediator-free glutamate sensors based on glutamate oxidase using smart micro-devices.
abstract: L-glutamate sensor based on glutamate oxidase (GLOx) has been fabricated on N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) activated thioglycolic acid (TGA) self-assembled monolayer (SAM) on smart bio-device using mediator-free simple phosphate buffer system (PBS), where TGA-SAM is used as an enzyme immobilization background. The glutamate sensor displayed a low detection limit (DL, 0.089 +/- 0.002 microM), a wide linear dynamic range (LDR, 0.1 microM to10.0 mM), good linearity (R = 0.9931), and higher sensitivity (20.75 +/- 1.0/microM)(-1)cm-2), small sample volume (70.0 microl), as well as good stability and reproducibility. The smart-biodevice system exemplified a simple and efficient approach to the assimilation of GLOx and sensing surface, which can provide analytical access to a large group of enzymes for wide range of bioelectrochemical applications in health care fields.
authors.affiliation: Venture Business Laboratory, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempeku-cho, Toyohashi, Aichi 441-8580, Japan.
authors.forename: M M
authors.initials: MM
authors.lastname: Rahman
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D018698:Glutamic Acid / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D018698:Glutamic Acid