title: Identification of a DNA methylation point in the promoter region of the bovine CYP21 gene.
abstract: The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu breeds by PCR-RFLP, and examined whether polymorphism in this element is associated with methylation. Among DNA samples from 135 Brazilian Zebu breed cattle, we identified an RFLP/HpaII polymorphism (T/C), which, based on a restriction methylation-sensitive assay employing HpaII and isoschizomer MspI enzymes (methylation-sensitive and -non-sensitive enzymes, respectively), appears to be a DNA methylation point. This is the first report of this polymorphism and on DNA methylation in the bovine CYP21 promoter region in Brazilian Zebu cattle.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: A M
authors.initials: AM
authors.lastname: da Silva
authors.forename: M A R
authors.initials: MA
authors.lastname: de Freitas
authors.forename: A F L
authors.initials: AF
authors.lastname: Rios
authors.forename: A
authors.initials: A
authors.lastname: Renzi
authors.forename: R B
authors.initials: RB
authors.lastname: Lôbo
authors.forename: M A V
authors.initials: MA
authors.lastname: Galerani
authors.forename: R A
authors.initials: RA
authors.lastname: Vila
authors.forename: E S
authors.initials: ES
authors.lastname: Ramos
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D019175:DNA Methylation / Q000235:genetics*; D057890:Epigenomics; D005838:Genotype; D011110:Polymorphism, Genetic / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013255:Steroid 21-Hydroxylase / Q000235:genetics*
chemical_list: D013255:Steroid 21-Hydroxylase