title: Isolation and chromatographic analysis of bioactive triterpenoids from the bark extract of Cariniana domestica (Mart) Miers.
abstract: The Lecythidaceae family is composed of 25 genera and 400 species that occur in the form of trees with a pantropical distribution. The genera Cariniana belongs to the family Lecythidaceae, and there are few reports considering these species. In this work, fractionation of the dichloromethane fraction obtained from the aqueous extract of the stem bark of Cariniana domestica (Mart) Miers, popularly known as Jequitibá-roxo, led to the isolation of two mixtures of triterpenoids: lupeol and β-amyrin and β-sitosterol and stigmasterol. The structures of the isolated compounds were elucidated by spectroscopic (NMR) and chromatographic (GC-MS) techniques as well as literature data comparisons. β-Sitosterol, stigmasterol, lupeol and β-amyrin were quantified in dichloromethane fraction by high-performance liquid chromatography (HPLC/DAD). The dichloromethane fraction was also investigated for antioxidant and antifungal activities. The isolated compounds and their biological activities are reported for the first time for the species C. domestica.
authors.affiliation: Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, Brazil. v.janovik@hotmail.com
authors.forename: Vanessa
authors.initials: V
authors.lastname: Janovik
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Janaína Kieling
authors.initials: JK
authors.lastname: Frohlich
authors.forename: Thiago Guilherme
authors.initials: TG
authors.lastname: Schwanz
authors.forename: Tarciele Venturini
authors.initials: TV
authors.lastname: Pozzebon
authors.forename: Sydney Hartz
authors.initials: SH
authors.lastname: Alves
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D031381:Lecythidaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008752:Methylene Chloride; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000032:analysis; D053978:Pentacyclic Triterpenes / Q000032:analysis; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D012855:Sitosterols / Q000032:analysis; D013265:Stigmasterol / Q000032:analysis; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000094042:Lupanes
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D008752:Methylene Chloride; D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D012855:Sitosterols; D013265:Stigmasterol; D014315:Triterpenes; C025473:gamma-sitosterol; C036380:beta-amyrin; C010480:lupeol; D000094042:Lupanes