title: Assessment of bioflocculant production by Bacillus sp. Gilbert, a marine bacterium isolated from the bottom sediment of Algoa Bay.
abstract: The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg²⁺ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Piyo
authors.initials: P
authors.lastname: Nontembiso
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Cosa
authors.initials: C
authors.lastname: Sekelwa
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Mabinya V
authors.initials: MV
authors.lastname: Leonard
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Okoh I
authors.identifier: 0000-0002-9770-085X
authors.initials: OI
authors.lastname: Anthony
mesh_terms: D000643:Ammonium Chloride / Q000737:chemistry / Q000378:metabolism; D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D061006:Bays; D002244:Carbon; D003470:Culture Media; D005427:Flocculation; D019015:Geologic Sediments / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D013019:South Africa; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D011108:Polymers; D011134:Polysaccharides; D012336:RNA, Ribosomal, 16S; D000643:Ammonium Chloride; D013395:Sucrose; D002244:Carbon; D009584:Nitrogen