title: Detection of DNA via the fluorescence quenching of Mn-doped ZnSe D-dots/doxorubicin/DNA ternary complexes system.
abstract: This manuscript reports a method for the detection of double-stranded DNA, based on Mn:ZnSe d-dots and intercalating agent doxorubicin (DOX). DOX can quench the photoluminescence (PL) of Mn:ZnSe d-dots through photoinduced electron transfer process, after binding with Mn:ZnSe d-dots. The addition of DNA can result in the formation of the Mn:ZnSe d-dots-DOX-DNA ternary complexes, the fluorescence of the Mn:ZnSe d-dots-DOX complexes would be further quenched by the addition of DNA, thus allowing the detection of DNA. The formation mechanism of the Mn:ZnSe d-dots-DOX-DNA ternary complexes was studied in detail in this paper. Under optimal conditions, the quenched fluorescence intensity of Mn:ZnSe d-dots-DOX system are perfectly described by Stern-Volmer equation with the concentration of hsDNA ranging from 0.006 μg mL(-1) to 6.4 μg mL(-1). The detection limit (S/N = 3) for hsDNA is 0.5 ng mL(-1). The proposed method was successfully applied to the detection of DNA in synthetic samples and the results were satisfactory.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Qianwei Road 10, Changchun, Jilin, 130012, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Niu
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D004579:Electron Transport; D008345:Manganese / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D018036:Selenium Compounds; D017967:Zinc Compounds; D008345:Manganese; D004317:Doxorubicin; D004247:DNA; C044696:zinc selenide