title: Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13.
abstract: Nowadays, majority of vanillin supplied to the world market is chemically synthesized from a petroleum-based raw material, raising a concern among the consumers regarding the product safety. In this study, an organic solvent-tolerant Brevibacillus agri 13 previously reported for a strong predilectic property was utilized as a whole-cell biocatalyst for bioproduction of vanillin from isoeugenol (IG). B. agri 13 is the first biocatalyst reported for bioproduction of vanillin at a temperature as high as 45°C. Both pH and temperature were found to affect vanillin production significantly. An extreme level of organic solvent tolerance of B. agri 13 allowed us to utilize it in a biphasic system using organic solvents generally considered as highly toxic to most bacteria. With an addition of butyl acetate at 30% (v/v) as an organic second phase, toxicity of IG exerted onto the biocatalyst was reduced dramatically while faster and more efficient vanillin production was obtained (1.7 g/L after 48 h with 27.8% molar conversion).
authors.affiliation: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
authors.forename: Nuttawat
authors.initials: N
authors.lastname: Wangrangsimagul
authors.forename: Kunticha
authors.initials: K
authors.lastname: Klinsakul
authors.forename: Alisa S
authors.initials: AS
authors.lastname: Vangnai
authors.forename: Jirarut
authors.initials: J
authors.lastname: Wongkongkatep
authors.forename: Pranee
authors.initials: P
authors.lastname: Inprakhon
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Honda
authors.forename: Hisao
authors.initials: H
authors.lastname: Ohtake
authors.forename: Junichi
authors.initials: J
authors.lastname: Kato
authors.forename: Thunyarat
authors.initials: T
authors.lastname: Pongtharangkul
mesh_terms: D000085:Acetates / Q000378:metabolism; D001547:Benzaldehydes / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D058675:Brevibacillus / Q000378:metabolism*; D004361:Drug Tolerance; D005054:Eugenol / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D012997:Solvents / Q000378:metabolism / Q000633:toxicity; D013696:Temperature
chemical_list: D000085:Acetates; D001547:Benzaldehydes; D012997:Solvents; D005054:Eugenol; C006848:butyl acetate; C036643:isoeugenol; C100058:vanillin