title: Determination of puerarin in rabbit aqueous humor by liquid chromatography tandem mass spectrometry using microdialysis sampling after topical administration of puerarin PAMAM dendrimer complex.
abstract: To study pharmacokinetic properties of puerarin poly(amido amine) (PAMAM) dendrimer complex, a sensitive liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated to determine puerarin in rabbit aqueous humor using microdialysis sampling. Astilbin was used as the internal standard. The linear range for puerarin was from 2 to 1000ng/mL (r=0.9986) based on 20μL of aqueous humor. The coefficients of variations for intra-day and inter-day precisions were less than 10.0%, and the relative error of accuracy was within ±6.3%. The mean extraction recovery of puerarin varied from 80.4% to 85.5%. Microdialysis provides a complete concentration versus time profile. A significant difference was observed in main pharmacokinetic parameters of C(max), AUC and t(1/2) between puerarin solution and puerarin PAMAM dendrimer complex. Complex formation resulted in an obvious increase in bioavailability of puerarin after topical administration to rabbit according to the above LC-MS/MS assay method.
authors.affiliation: School of Pharmacy, Yantai University, Yantai 264005, China; State Key Laboratory of Long-acting and Targeting Drug Delivery System, Yantai, China.
authors.forename: Wen-Yan
authors.initials: WY
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Yao
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Shao
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Mu
authors.forename: Kao-Xiang
authors.initials: KX
authors.lastname: Sun
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry*; D001672:Biocompatible Materials / Q000032:analysis* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods; D050091:Dendrimers / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D004338:Drug Combinations; D004355:Drug Stability; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D017551:Microdialysis / Q000379:methods; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014665:Vasodilator Agents / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D001672:Biocompatible Materials; D050091:Dendrimers; D004338:Drug Combinations; D007529:Isoflavones; C104700:PAMAM Starburst; D014665:Vasodilator Agents; C033607:puerarin