title: Effects of Salivae Miltiorrhizae Liguspyragine Hydrochloride and Glucose Injection on the levels of main platelet thrombin receptors in chronic haemodialysis patients.
abstract: OBJECTIVE
  To investigate the effects of Salvia Miltiorrhiza Liguspyragine Hydrochloride and Glucose Injection (, SLGI) on the expression of platelet membrane receptors proteinase-activated receptor-1 (PAR1) and proteinase-activated receptor-4 (PAR4) in end-stage renal disease (ESRD) patients on chronic haemodialysis (HD).
  
  
  METHODS
  Eighty-six ESRD patients on HD (treated group) were treated with SLGI, 7 days as one therapeutic course, for two successive courses. The previous therapies were unchanged. Flow cytometry was used to assess the expression of platelet PAR1 and PAR4 in the patients, and turbidity method was used to determine the platelet maximum aggregation rate (MAR). Meanwhile, renal function was measured. The final data were compared with those before treatment and with those in the normal control group (54 healthy subjects).
  
  
  RESULTS
  Compared with the normal control group, the expressions of PAR1 and PAR4 and platelet MAR in ESRD patients on HD was significantly higher before treatment (P=0.001, P=0.006, and P=0.008); after treatment with SLGI, the above indices in patients were remarkably decreased (P=0.036 and P=0.046), except PAR4 (P=0.067), but still higher than those in the normal control group, however, it was not statistically significant.
  
  
  CONCLUSIONS
  (1) The overexpression of PAR1 and PAR4 might lead to increased platelet aggregation and this could be one of the reasons for the thrombotic events in ESRD patients on HD. (2) SLGI was able to down-regulate the expression of PAR1 in ESRD patients on HD, improve platelet function, and regulate platelet activation.
authors.affiliation: Department of Integrated Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Lu
authors.forename: You
authors.initials: Y
authors.lastname: Qin
authors.forename: Jan-guo
authors.initials: JG
authors.lastname: Liu
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000009:adverse effects / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D005947:Glucose / Q000008:administration & dosage* / Q000494:pharmacology; D006801:Humans; D007677:Kidney Function Tests; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D018179:Receptors, Thrombin / Q000378:metabolism*; D006435:Renal Dialysis; D027561:Salvia miltiorrhiza / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D018179:Receptors, Thrombin; D005947:Glucose