title: [Study on extraction and purification techniques and free radical scavenging activity of total flavonoids from Fissistigma oldhamii].
abstract: OBJECTIVE
  To optimize the extraction and purification techniques of total flavonoids in Fissistigma oldhamii. The scavenging activity to DPPH of the total flavonoids and the extraction were studied in vitro.
  
  
  METHODS
  By orthogonal design, we investigated the effects of concentration of ethanol, temperature, ratio of solid to liquid and time of extraction on the total flavonoids extraction rate. The total flavonoids was separated and purified by AB-8 macroporous resin absorption.
  
  
  RESULTS
  The optimum extraction condition was that at extraction time of 3 h, concentration of alcohol 90%, solid/liquid ratio of 1:10 and temperature of 70 degrees C. The content of total flavonoids in the refined product was above 55%. The GFM-2 fraction showed antioxidant capacity in vitro.
  
  
  CONCLUSIONS
  The optimum extraction condition and purification experiment are very useful for further development of industrial process. Meantime, the result may be helpful for further development of Fissistigma oldhamii as health protection foods.
authors.affiliation: Department of Pharmacy, Shaoyang Junior Medicine College, Shaoyang 422000, China. 2. Red Cross Hospital at Xinshao County of. chunyanfull@yahoo.com.cn
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Fu
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Dai-Wu
authors.initials: DW
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Tang
authors.forename: Fen
authors.initials: F
authors.lastname: Feng
authors.forename: Zhong-Liu
authors.initials: ZL
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D029061:Annonaceae / Q000737:chemistry*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D000431:Ethanol; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D012117:Resins, Synthetic; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D012117:Resins, Synthetic; D012997:Solvents; D000431:Ethanol; C004931:1,1-diphenyl-2-picrylhydrazyl