title: [Investigation on correlation between ratio of xylem to phloem of Radix Isatidis and efficacy, chemical composition].
abstract: OBJECTIVE
  Explore contribution of ratio of xylem to phloem(RXP) to evaluate the quality of Radix Isatidis.
  
  
  METHODS
  Antivirus activity and chemical compositions of xylem, phloem and Radix Isatidis of different RXP were determined by RBC agglutination test and unique chromatogram. Meanwhile, correlation between RXP and bioactivity,components was investigated.
  
  
  RESULTS
  the activity of medical material of Radix Isatidis whose RXP was 1:2 or 1:1 is equal to that of phloem sample, while is stronger than that of cylem sample. There was a good consistency among the chemical figureprints of three samples (Radix Isatidis, xylem and phloem). When the RXP was 2:1, the medical material of Radix Isatidi and its xylem had the same activity. But the activity of phloem was not obvious. Their consistency of chemical fingerprint was bad, and the activity of Radix Isatidis which had RXP of 1:2 or 1:1 was better than that formed by xylem and phloem of 2:1. The Radix Isatidis of RXP of 1:2 or 1:1 had less similarity of chemical figureprint with that having RXP of 2:1.
  
  
  CONCLUSIONS
  The quality of Radix Isatidis made up by the various RXP had significant difference. Radix Isatidis whose RXP is less than 1:1 had good quality and better activity. As a characteristic parameter of biologic morpha, the RXP can be applied to identifying the quality of Radix Isatidis, and also provided a reference to evaluation of other medical material of roots.
authors.affiliation: Institute of Chinese Medicine, 302 Hospital of People's Liberation Army, Beijing 100039, China. yd277@126.com
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Han
authors.forename: Jiao-yang
authors.initials: JY
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Shao-feng
authors.initials: SF
authors.lastname: Zhang
authors.forename: Xiao-he
authors.initials: XH
authors.lastname: Xiao
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology; D031219:Isatis / Q000033:anatomy & histology / Q000737:chemistry*; D052585:Phloem / Q000737:chemistry*; D018517:Plant Roots / Q000033:anatomy & histology / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D052584:Xylem / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal