title: Chiral imprinted polymers as enantiospecific coatings of stir bar sorptive extraction devices.
abstract: This paper reports the design of Molecularly Imprinted Polymers (MIP) with affinity towards (S)-citalopram using computational modeling for the selection of functional monomers and monomer:template ratio. Acrylamide was selected as functional monomer and the final complex functional monomer/template resulted in a 3:1 ratio. The polymer was synthesized by radical polymerization initiated by UV onto magnetic stir-bars in order to obtain a stir bar sorptive extraction (SBSE) device capable of selective enantiomeric recognition. After successful template removal, the parameters affecting the SBSE procedure (sample volume, ionic strength, extraction time and pH) were optimized for the effective rebinding of the target analyte. The resultant chirally imprinted polymer based stir-bar was able to selectively extract (S)-citalopram from a racemic mixture in an aqueous media with high specificity (specificity factor 4) between 25 and 500 μgL(-1). The MIP coated stir-bars can have significance for enantiospecific sample pre-concentration and subsequent analysis without the need for any chiral chromatographic separation.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, University of the Basque Country, 01006 Vitoria-Gasteiz, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Gomez-Caballero
authors.forename: Antonio
authors.initials: A
authors.lastname: Guerreiro
authors.forename: Kal
authors.initials: K
authors.lastname: Karim
authors.forename: Sergey
authors.initials: S
authors.lastname: Piletsky
authors.forename: M Aranzazu
authors.initials: MA
authors.lastname: Goicolea
authors.forename: Ramon J
authors.initials: RJ
authors.lastname: Barrio
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation* / Q000379:methods; D015283:Citalopram / Q000032:analysis*; D003198:Computer Simulation; D054802:Molecular Imprinting; D013237:Stereoisomerism
chemical_list: D015283:Citalopram