title: Comparative expression profiles for KiSS-1 and REN genes in preeclamptic and healthy placental tissues.
abstract: OBJECTIVE
  The aim of the present work was to look at differences in the placental tissue expression of KiSS-1 and REN genes from preeclamptic and healthy pregnant women, that could account for a possible synergistic function for both genes in the pathogenesis of preeclampsia.
  
  
  METHODS
  This case-control study involved 27 preeclamptic women and 27 normoevolutive pregnant women. cDNA was obtained from placental tissue to carry out qPCR for both KiSS-1 and REN genes in order to compare mRNA expression levels in the studied groups. Statistical analysis showed expression differences that correlate with clinical and/or biochemical variables.
  
  
  RESULTS
  Higher expression for KiSS-1 in PEE vs. control woman (p=0.001) was observed, whereas no difference was observed for REN expression (p=0.300) when all the subjects were included. However, REN expression was significant higher when the samples were stratified according to preeclampsia severity. For 18 mild preeclamptic patients the p-value was p=0.001 compared to their controls, while for the remaining nine with severe preeclampsia the expression became significant (p=0.001).
  
  
  CONCLUSIONS
  Our results suggest that the high KiSS-1 expression seen in preeclamptic patients is in accordance with its role as an inhibitor of trophoblast invasiveness and maintained until the end of gestation. On the other hand, aggressive therapeutic management and/or severity status of patients have a direct effect on placental REN expression levels, masking the natural high expression of this gene on preeclamptic placental tissue. Therefore it was not possible to establish a real concordant expression profile for KiSS-1 and REN genes.
authors.affiliation: Academia de Genómica, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional, IPN Unidad Dgo., Durango, Zip Code 34220, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Vazquez-Alaniz
authors.forename: Carlos
authors.initials: C
authors.lastname: Galaviz-Hernandez
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Marchat
authors.forename: José M
authors.initials: JM
authors.lastname: Salas-Pacheco
authors.forename: Isaías
authors.initials: I
authors.lastname: Chairez-Hernandez
authors.forename: José J
authors.initials: JJ
authors.lastname: Guijarro-Bustillos
authors.forename: Alberto
authors.initials: A
authors.lastname: Mireles-Ordaz
mesh_terms: D000293:Adolescent; D000328:Adult; D000959:Antihypertensive Agents / Q000627:therapeutic use; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D006973:Hypertension / Q000209:etiology; D059648:Kisspeptins / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology / Q000652:urine; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D011507:Proteinuria / Q000209:etiology; D012333:RNA, Messenger / Q000378:metabolism; D012083:Renin / Q000235:genetics / Q000378:metabolism*; D012720:Severity of Illness Index; D015854:Up-Regulation / Q000187:drug effects; D055815:Young Adult
chemical_list: D000959:Antihypertensive Agents; C106628:KISS1 protein, human; D059648:Kisspeptins; D011257:Pregnancy Proteins; D012333:RNA, Messenger; D012083:Renin