title: Genotypic and phenotypic differences between nodal and extranodal diffuse large B-Cell lymphomas.
abstract: Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of diseases that have diverse clinical, pathological, and biological features. Here, it is shown that primary nodal and extranodal DLBCLs differ genomically and phenotypically. Using conventional comparative genomic hybridization (CGH), the authors assessed the chromosomal aberrations in 18 nodal, 13 extranodal, and 5 mixed DLBCLs. The results demonstrate significantly distinct chromosomal aberrations exemplified by gains of chromosomal arms 1p, 7p, 12q24.21-12q24.31, and 22q and chromosome X and loss of chromosome 4, 6q, and 18q22.3-23 in extranodal compared with nodal DLBCLs. Nodal DLBCLs showed an increased tendency for 18q amplification and BCL2 protein overexpression compared with extranodal and mixed tumors. Using a panel of five antibodies against GCET1, MUM1, CD10, BCL6, and FOXP1 proteins to subclassify DLBCLs according to the recent Choi algorithm, the authors showed that the genomic profiles observed between the nodal and extranodal DLBCLs were not due to the different proportions of GCB vs ABC in the two groups. Further delineation of these genomic differences was illuminated by the use of high-resolution 21K BAC array CGH performed on 12 independent new cases of extranodal DLBCL. The authors demonstrated for the first time a novel genome and proteome-based signatures that may differentiate the two lymphoma types.
authors.affiliation: Department of Pathology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
authors.forename: Salah A
authors.initials: SA
authors.lastname: Al-Humood
authors.forename: Aisha S
authors.initials: AS
authors.lastname: Al-Qallaf
authors.forename: Salem H
authors.initials: SH
authors.lastname: Alshemmari
authors.forename: Issam M
authors.initials: IM
authors.lastname: Francis
authors.forename: Thamradeen A
authors.initials: TA
authors.lastname: Junaid
authors.forename: Rajaa A
authors.initials: RA
authors.lastname: Marouf
authors.forename: Fahd
authors.initials: F
authors.lastname: Al-Mulla
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000465:Algorithms; D002648:Child; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000235:genetics; D015331:Cohort Studies; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D050835:Interferon Regulatory Factors / Q000378:metabolism; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D015260:Neprilysin / Q000378:metabolism; D040901:Proteomics; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins / Q000378:metabolism; D012189:Retrospective Studies; D015843:Serpins / Q000378:metabolism; D055815:Young Adult; D000099218:Interferon Regulatory Factor-4
chemical_list: D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D050835:Interferon Regulatory Factors; D009363:Neoplasm Proteins; D015260:Neprilysin; D051560:Proto-Oncogene Proteins c-bcl-6; D012097:Repressor Proteins; D015843:Serpins; D000099218:Interferon Regulatory Factor-4; C084103:BCL6 protein, human; C486626:FOXP1 protein, human; C476825:SERPINA9 protein, human