title: Impaired mobilization of CD133(+) bone marrow-derived circulating progenitor cells with an increased number of diseased coronary arteries in ischemic heart disease patients with diabetes.
abstract: BACKGROUND
  The influence of the number of diseased coronary arteries on the mobilization of CD133/45(+) bone marrow-derived circulating progenitor cells (BM-CPCs) in peripheral blood (PB) in patients with ischemic heart disease (IHD) was analyzed.
  
  
  RESULTS
  Mobilization of CD133/45(+) BM-CPCs by flow cytometry was measured in 120 patients with coronary 1 vessel (IHD1, n=40), coronary 2 vessel (IHD2, n=40), and coronary 3 vessel disease (IHD3, n=40), and in a control group (n=40). The mobilization of CD133/45(+) BM-CPCs was significantly reduced in patients with IHD compared to the control group (P<0.001). The mobilization of CD133/45(+) BM-CPCs was impaired in patients with IHD3 compared to IHD1 (P<0.001) and to IHD2 (P<0.001). But there was no significant difference in mobilization of CD133/45(+) BM-CPCs between the patients with IHD2 and IHD1 (P=0.35). Moreover, we found significantly reduced CD133/45(+) cell mobilization in patients with a high SYNTAX-Score (SS) compared to a low SS (P<0.001) and an intermediate SS (P<0.001). In subgroup analyzes, we observed a significantly negative correlation between levels of hemoglobin A(1c) and the mobilization of CD133/45(+) BM-CPCs (P=0.001, r=-0.6).
  
  
  CONCLUSIONS
  The mobilization of CD133/45(+) BM-CPCs in PB is impaired in patients with IHD. This impairment might augment with increased number of diseased coronary arteries. Moreover, mobilization of CD133/45(+) BM-CPCs in ischemic tissue is further impaired by diabetes in patients with IHD.
authors.affiliation: Department of Internal Medicine, Division of Cardiology, University Hospital Rostock, Germany. dr_g_turan@hotmail.com
authors.forename: R Goekmen
authors.initials: RG
authors.lastname: Turan
authors.forename: C Hakan
authors.initials: CH
authors.lastname: Turan
authors.forename: Ilkay
authors.initials: I
authors.lastname: Bozdag-Turan
authors.forename: Jasmin
authors.initials: J
authors.lastname: Ortak
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Akin
authors.forename: Stephan
authors.initials: S
authors.lastname: Kische
authors.forename: Henrik
authors.initials: H
authors.lastname: Schneider
authors.forename: Tilo
authors.initials: T
authors.lastname: Kleinfeldt
authors.forename: Tim C
authors.initials: TC
authors.lastname: Rehders
authors.forename: Mathias
authors.initials: M
authors.lastname: Rauchhaus
authors.forename: Esther
authors.initials: E
authors.lastname: Adolph
authors.forename: Shwan
authors.initials: S
authors.lastname: Amen
authors.forename: Tina
authors.initials: T
authors.lastname: Hermann
authors.forename: Sedat
authors.initials: S
authors.lastname: Yokus
authors.forename: Micheal
authors.initials: M
authors.lastname: Brehm
authors.forename: Stephan
authors.initials: S
authors.lastname: Steiner
authors.forename: Kurtulus
authors.initials: K
authors.lastname: Sahin
authors.forename: Christoph A
authors.initials: CA
authors.lastname: Nienaber
authors.forename: Hueseyin
authors.initials: H
authors.lastname: Ince
mesh_terms: D000071916:AC133 Antigen; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D001854:Bone Marrow Cells; D048909:Diabetes Complications / Q000097:blood* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006023:Glycoproteins; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D017493:Leukocyte Common Antigens; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood* / Q000473:pathology; D010455:Peptides; D013234:Stem Cells
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human