title: Establishment of a novel MALT lymphoma cell line, ma-1, from a patient with t(14;18)(q32;q21)-positive Helicobacter pylori-independent gastric MALT lymphoma.
abstract: Although t(11;18)(q21;q21), t(1;14)(p22;q32), and a few other genetic mutations are specific markers for the Helicobacter pylori (HP)-independent status of gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the molecular mechanisms responsible for HP-independence of gastric MALT lymphoma without such translocations and mutations remain uncharacterized. In the present study, we describe the establishment and characterization of a novel MALT lymphoma cell line, MA-1, which was derived from a gastric MALT lymphoma which was negative for both t(11;18)(q21;q21) and t(1;14)(p22;q32); the patient had failed HP eradication therapy and chemotherapy. The cell morphology and the immunophenotype of this cell line were similar to that of the original gastric MALT lymphoma. Comparative genomic hybridization analysis showed no significant gene copy number changes. Spectral karyotyping displayed a near-diploid chromosome content (48 < 2n>XY), with at least 13 chromosome structural abnormalities. Furthermore, fluorescence in situ hybridization analyses disclosed the existence of three sub-clones, characterized by t(14;18)(q32;q21)/IGH-BCL2, t(14;18)(q32;q21)/IGH-MALT1, and the presence of both chromosomal translocations in the same cell, respectively; whereas amplification of the genes CRAD9, TRAF2, and BCL10 were not found. In conclusion, we have established the first human gastric MALT lymphoma cell line, which is characterized by unusual and complex chromosome translocations and will be useful to explore further the molecular mechanisms of HP-independence in gastric MALT lymphoma.
authors.affiliation: Department of Oncology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei.
authors.forename: Sung-Hsin
authors.initials: SH
authors.lastname: Kuo
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Weng
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Ping-Ning
authors.initials: PN
authors.lastname: Hsu
authors.forename: Ming-Shiang
authors.initials: MS
authors.lastname: Wu
authors.forename: Chung-Wu
authors.initials: CW
authors.lastname: Lin
authors.forename: Huei-Jiuan
authors.initials: HJ
authors.lastname: Jeng
authors.forename: Kun-Huei
authors.initials: KH
authors.lastname: Yeh
authors.forename: Hui-Jen
authors.initials: HJ
authors.lastname: Tsai
authors.forename: Li-Tzong
authors.initials: LT
authors.lastname: Chen
authors.forename: Ann-Lii
authors.initials: AL
authors.lastname: Cheng
mesh_terms: D045744:Cell Line, Tumor; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D055028:Comparative Genomic Hybridization; D005434:Flow Cytometry; D018628:Gene Dosage; D016481:Helicobacter Infections / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D018442:Lymphoma, B-Cell, Marginal Zone / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D032681:Spectral Karyotyping; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D014178:Translocation, Genetic