title: Development of microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae) and cross-amplification in Cephalotaxus.
abstract: OBJECTIVE
  Microsatellite loci were developed for Cephalotaxus oliveri, an endemic and endangered conifer in China, which will allow assessment of the levels of genetic diversity and a means to understand the genetic consequences of habitat fragmentation.
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 19 microsatellite loci were identified in C. oliveri, 13 of which were polymorphic within a sample of 52 individuals representing five natural populations. The actual number of alleles per locus ranged from one to five. Twelve polymorphic loci were also successfully amplified in C. fortunei.
  
  
  CONCLUSIONS
  These microsatellite loci will provide a useful tool for further investigating genetic variation in natural populations of C. oliveri, which will inform future conservation and management strategies. Additionally, cross-amplification in C. fortunei suggested the potential utility of these loci in this and other congeneric species.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Pan
authors.forename: Ying-Rong
authors.initials: YR
authors.lastname: Guo
authors.forename: Ying-Juan
authors.initials: YJ
authors.lastname: Su
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D032483:Cephalotaxus / Q000235:genetics* / Q000378:metabolism; D002681:China; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D017753:Ecosystem; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005787:Gene Frequency; D006580:Genetic Carrier Screening / Q000379:methods; D056426:Genetic Loci; D018745:Genome, Plant; D006579:Heterozygote; D018895:Microsatellite Repeats; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant