title: MicroRNA let-7e regulates the expression of caspase-3 during apoptosis of PC12 cells following anoxia/reoxygenation injury.
abstract: This study aimed to investigate the role and mechanism of action of microRNA (miR) let-7e in PC12 cells undergoing apoptosis following anoxia/reoxygenation (A/R) injury. The putative binding site of let-7e in the 3' UTR of caspase-3 (Casp3) mRNA was analyzed using the miRanda algorithm. Precursor let-7e (pre-miRNA), let-7e miR and anti-let-7e oligonucleotides were transfected into PC12 cells, which were then subjected to A/R injury. The levels of Casp3 mRNA and let-7e miRNA, the total protein levels of Casp3, Casp8 and Casp9 and levels of cellular apoptosis were measured. It was found that let-7e expression in PC12 cells was decreased, whereas the expression of Casp3 was significantly increased after A/R injury. The transfection of pre-miRNA or let-7e miR into PC12 cells decreased Casp3 expression levels and cellular apoptosis following A/R injury, while co-transfection of anti-let-7e strikingly alleviated the effects of let-7e miR. These results indicate that let-7e may protect PC12 cells against apoptosis following A/R injury by negatively regulating the expression of Casp3.
authors.affiliation: Department of Neurology, Medical College of Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Peng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Qianyi
authors.initials: Q
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Ben-Yan
authors.initials: BY
authors.lastname: Luo
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D053148:Caspase 3 / Q000096:biosynthesis*; D053181:Caspase 8 / Q000096:biosynthesis; D053453:Caspase 9 / Q000096:biosynthesis; D015687:Cell Hypoxia / Q000187:drug effects*; D035683:MicroRNAs / Q000502:physiology*; D010100:Oxygen / Q000633:toxicity*; D016716:PC12 Cells; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D010100:Oxygen