title: [Examination of warm and cold ischemic injury of small intestine by differential scanning calorimetry].
abstract: OBJECTIVE
  Small bowel is extremely sensitive to ischemia/reperfusion injury. Therefore, we compared the effect of warm and cold ischemia on the intestinal structural changes by differential scanning calorimetry (DSC) method.
  
  
  METHODS
  Warm and cold ischemia groups were established on Wistar rats with 1, 3 and 6 h ischemic times (n = 30). Intestinal biopsies were collected after laparotomy and at the end of the ischemia periods. DSC measurement was performed on the mucosa, muscular layer and total intestinal wall.
  
  
  RESULTS
  Our DSC data confirmed that longer warm ischemia period caused more severe damage in the structure of the mucosa and muscular layers. The results of transition temperature and calorimetric enthalpy suggest that these changes can be reduced using cold ischemic procedure in University of Wisconsin solution. However, the extent of thermal destruction of each layers following cold preservation injury was significantly different compared to normal bowel structure.
  
  
  CONCLUSIONS
  In this study we quantitatively measured structural changes in the gut following ischemia using DSC. This thermodynamic method may provide basis for further investigations in different bowel stress models.
authors.affiliation: Zala Megyei Kórház Általános Sebészeti Osztály 8900 Zalaegerszeg Zrínyi u. 1.
authors.forename: Klára
authors.initials: K
authors.lastname: Nedvig
authors.forename: István
authors.initials: I
authors.lastname: Zapf
authors.forename: Tamás
authors.initials: T
authors.lastname: Fekecs
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D003080:Cold Temperature; D007421:Intestine, Small; D007422:Intestines; D017208:Rats, Wistar; D015427:Reperfusion Injury