title: Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering.
abstract: A surface modification technique based on poly(dopamine) deposition developed from oxidative polymerization of dopamine is known to promote cell adhesion to several cell-resistant substrates. In this study this technique was applied to articular cartilage tissue engineering. The adhesion and proliferation of rabbit chondrocytes were evaluated on poly(dopamine)-coated polymer films, such as polycaprolactone, poly(L-lactide), poly(lactic-co-glycolic acid) and polyurethane, biodegradable polymers that are commonly used in tissue engineering. Cell adhesion was significantly increased by merely 15 s of dopamine incubation, and 4 min incubation was enough to reach maximal cell adhesion, a 1.35-2.69-fold increase compared with that on the untreated substrates. Cells also grew much faster on the poly(dopamine)-coated substrates than on untreated substrates. The increase in cell affinity for poly(dopamine)-coated substrates was demonstrated via enhancement of the immobilization of serum adhesive proteins such as fibronectin. When the poly(dopamine)-coating technique was applied to three-dimensional (3-D) polyurethane scaffolds, the proliferation of chondrocytes and the secretion of glycosaminoglycans were increased compared with untreated scaffolds. Our results show that the deposition of a poly(dopamine) layer on 3-D porous scaffolds is a simple and promising strategy for articular cartilage tissue engineering, and may be applied to other types of tissue engineering.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, 1 Roosevelt Road, Section 4, Taipei 106, Taiwan. weibortsai@ntu.edu.tw
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
authors.forename: Wen-Tung
authors.initials: WT
authors.lastname: Chen
authors.forename: Hsiu-Wen
authors.initials: HW
authors.lastname: Chien
authors.forename: Wei-Hsuan
authors.initials: WH
authors.lastname: Kuo
authors.forename: Meng-Jiy
authors.initials: MJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D023822:Tissue Engineering
chemical_list: D007211:Indoles; D011108:Polymers; C568283:polydopamine