title: A single major QTL controls expression of larval Cry1F resistance trait in Ostrinia nubilalis (Lepidoptera: Crambidae) and is independent of midgut receptor genes.
abstract: The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is an introduced crop pest in North America that causes major damage to corn and reduces yield of food, feed, and biofuel materials. The Cry1F toxin from Bacillus thuringiensis (Bt) expressed in transgenic hybrid corn is highly toxic to O. nubilalis larvae and effective in minimizing feeding damage. A laboratory colony of O. nubilalis was selected for high levels of Cry1F resistance (>12,000-fold compared to susceptible larvae) and is capable of survival on transgenic hybrid corn. Genetic linkage maps with segregating AFLP markers show that the Cry1F resistance trait is controlled by a single quantitative trait locus (QTL) on linkage group 12. The map position of single nucleotide polymorphism (SNP) markers indicated that midgut Bt toxin-receptor genes, alkaline phosphatase, aminopeptidase N, and cadherin, are not linked with the Cry1F QTL. Evidence suggests that genes within this genome interval may give rise to a novel Bt toxin resistance trait for Lepidoptera that appears independent of known receptor-based mechanisms of resistance.
authors.affiliation: USDA-ARS, Corn Insects & Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011, USA. brad.coates@ars.udsa.gov
authors.forename: Brad S
authors.initials: BS
authors.lastname: Coates
authors.forename: Douglas V
authors.initials: DV
authors.lastname: Sumerford
authors.forename: Miriam D
authors.initials: MD
authors.lastname: Lopez
authors.forename: Haichuan
authors.initials: H
authors.lastname: Wang
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Fraser
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Kroemer
authors.forename: Terrence
authors.initials: T
authors.lastname: Spencer
authors.forename: Kyung S
authors.initials: KS
authors.lastname: Kim
authors.forename: Craig A
authors.initials: CA
authors.lastname: Abel
authors.forename: Richard L
authors.initials: RL
authors.lastname: Hellmich
authors.forename: Blair D
authors.initials: BD
authors.lastname: Siegfried
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics / Q000633:toxicity*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D007915:Lepidoptera / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D008297:Male; D010375:Pedigree; D010641:Phenotype; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*
chemical_list: D001427:Bacterial Toxins; D019476:Insect Proteins; D004247:DNA