title: [Studies on fingerprints of Centella asiatica by HPLC].
abstract: OBJECTIVE
  To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Centella asiatica.
  
  
  METHODS
  HPLC gradient elution was applied for the fingerprints of Centella asiatica. All 16 samples are collected from different habitats of China. The columni was Alltech C18 column (250 mm x 4.6 mm, 5 microm), mobile phase was acetonitrile-water, flow rate was 1.0 ml/min, wavelength was 205 nm.
  
  
  RESULTS
  The fingerprint of Centella asiatica was established, 16 samples of different areas of Centella asiatica were detected. There were 15 common peaks in the HPLC fingerprints of Centella asiatica. By comparison with the reference standards and using LC-ESI-MS(n) to corroborate the structure, 5-10 peaks were identified as madecassoside, asiaticoside, quercetin, kaemperol, madecassic acid and asiatic acid respectively. After calculating the similarity of the HPLC fingerprints of 16 habitants, the similarity of different habitats has been bad quite.
  
  
  CONCLUSIONS
  The method is accurate, reliable and good repeatability. This chromatographic fingerprint method can be used to controll the quality of Centella asiatica.
authors.affiliation: Affiliated Hospital of NingXia Medical University, Yinchuan 750004, China. saiint@163.com
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Wan-hong
authors.initials: WH
authors.lastname: Li
authors.forename: Shi-qiang
authors.initials: SQ
authors.lastname: Hu
mesh_terms: D028041:Centella / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry* / Q000592:standards; D010598:Pharmacognosy; D010946:Plants, Medicinal / Q000737:chemistry / Q000254:growth & development; D011786:Quality Control; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D014315:Triterpenes; D011794:Quercetin; C001669:madecassic acid