title: PEA3 activates CXCR4 transcription in MDA-MB-231 and MCF7 breast cancer cells.
abstract: CXC chemokine receptor 4 (CXCR4) is a cell surface receptor that has been shown to mediate the metastasis of many solid tumors including lung, breast, kidney, and prostate tumors. In this study, we found that overexpression of ets variant gene 4 (PEA3) could elevate CXCR4 mRNA level and CXCR4 promoter activity in human MDA-MB-231 and MCF-7 breast cancer cells. PEA3 promoted CXCR4 expression and breast cancer metastasis. Chromatin immunoprecipitation assay demonstrated that PEA3 could bind to the CXCR4 promoter in the cells transfected with PEA3 expression vector. PEA3 siRNA attenuated CXCR4 promoter activity and the binding of PEA3 to the CXCR4 promoter in MDA-MB-231 and MCF-7 cells. These results indicated that PEA3 could activate CXCR4 promoter transcription and promote breast cancer metastasis.
authors.affiliation: Department of Breast Surgery, Breast Cancer Institute, Department of Oncology, Shanghai Medical College, Institute of Biomedical Science, Fudan University, China.
authors.forename: Shengmei
authors.initials: S
authors.lastname: Gu
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Hong
authors.forename: Tingting
authors.initials: T
authors.lastname: Yan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhuang
authors.forename: Qiaoqiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.forename: Jiong
authors.initials: J
authors.lastname: Wu
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D034741:RNA, Small Interfering; D019718:Receptors, CXCR4; D014157:Transcription Factors; C065788:transcription factor PEA3