title: [A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo].
abstract: To perform electrophysiological recording and other investigations on transplanted neural cells in vivo, we used mechanical damage to establish a special traumatic brain injury model that could distinguish transplanted cells from host cells. The morphology of the trauma-induced holes in the cortex of the rat brain was regular. The model was stable and repeatable. Neural stem cells were transplanted into the trauma-induced hole, and were able to survive for a long time. Most of the transplanted cells differentiated into neurons, and only a small amount turned into glia cells. There was a clear boundary between the host cells and the transplanted cells. Single cell electrophysiological recording on transplanted neural cells were detected in vivo. This study established a stable and repeatable traumatic brain injury model, which could be used to conduct in vivo electrophysiological recording research on transplanted neural cells.
authors.affiliation: State Kay Laboratory of Brain and Cognitive Science, Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100101, China. Yangsc@mail.kiz.ac.cn
authors.forename: Shang-Chuan
authors.initials: SC
authors.lastname: Yang
authors.forename: Jin-Run
authors.initials: JR
authors.lastname: Dong
authors.forename: Jia-Gui
authors.initials: JG
authors.lastname: Qu
authors.forename: Xin-Tian
authors.initials: XT
authors.lastname: Hu
authors.forename: Zheng-Bo
authors.initials: ZB
authors.lastname: Wang
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology / Q000628:therapy*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena; D005260:Female; D006801:Humans; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000502:physiology / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000502:physiology; D019737:Transplants