title: Free radical scavenging activity, determination of phenolic compounds and HPLC-DAD/ESI-MS profile of Campomanesia adamantium leaves.
abstract: Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activity to scavenge free radicals. Campomanesia adamantium (Myrtaceae) is a small tree with edible fruit, commonly known as "guavira" or "guabiroba-branca" that has been used in popular medicine as depurative anti-diarrhoeic, antiinflammatory, anti-rheumatic and to liver diseases. In this study, the antiradical activities of ethanol crude extract of the leaves from C. adamantium and the ethyl acetate and butanol fractions obtained by partition, were determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl radical) and ORAC-FL (Oxygen Radical Absorbance Capacity) assays. The total phenol content in the samples was estimated by Folin Ciocalteau method (FCR). In an initial evaluation the ethanolic extract and the fractions ethyl acetate and butanol have shown levels of phenolic compounds between 15- 74 mg GAE/g in FCR assay, showed DPPH free-radical scavenging activity with SC50 in the range of 7.77-13.35 microg/mL and demonstrated antioxidant capacity between 2648-3502 micromol TE/g of extract and fractions in the ORAC-FL assay. HPLC-DAD and ESI-MS analysis revealed were that the extract of the leaves of C. adamantium studied appears to contain flavonoids as major constituents, including isoquercetrin and quercetin that exhibit proven antioxidant activity.
authors.affiliation: Pharmacy School, Department of Plant Biology, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP 13083-970, Brazil.
authors.forename: Aislan C R F
authors.initials: AC
authors.lastname: Pascoal
authors.forename: Carlos Augusto
authors.initials: CA
authors.lastname: Ehrenfried
authors.forename: Marcos N
authors.initials: MN
authors.lastname: Eberline
authors.forename: Maria Elida Alves
authors.initials: ME
authors.lastname: Stefanello
authors.forename: Marcos José
authors.initials: MJ
authors.lastname: Salvador
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D001938:Brazil; D002839:Chromans / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D027822:Myrtaceae / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D001713:Biphenyl Compounds; D002839:Chromans; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid