title: Development and characterization of polymorphic microsatellite markers in Dysosma pleiantha (Berberidaceae).
abstract: OBJECTIVE
  The development of compound microsatellite markers was conducted in Dysosma pleiantha to investigate genetic diversity and population genetic structure of this threatened medicinal plant.
  
  
  RESULTS
  Using the compound microsatellite marker technique, 14 microsatellite markers that were successfully amplified showed polymorphism when tested on 38 individuals from three populations in eastern China. Overall, the number of alleles per locus ranged from 2 to 14, with an average of 7.71 alleles per locus.
  
  
  CONCLUSIONS
  These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure and genetic diversity in Dysosma pleiantha.
authors.affiliation: College of Life Sciences and Food Engineering, Nanchang University, Nanchang 310031, China. guanbicai12@163.com
authors.forename: Bi-Cai
authors.initials: BC
authors.lastname: Guan
authors.forename: Xi
authors.initials: X
authors.lastname: Gong
authors.forename: Shi-Liang
authors.initials: SL
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D001483:Base Sequence; D028141:Berberidaceae / Q000235:genetics* / Q000378:metabolism; D002681:China; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D056727:Endangered Species; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005787:Gene Frequency; D015723:Gene Library; D006580:Genetic Carrier Screening / Q000379:methods*; D056426:Genetic Loci; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant