title: Immunoassay of C-reactive protein by hot electron induced electrochemiluminescence using integrated electrodes with hydrophobic sample confinement.
abstract: C-reactive protein (CRP) was determined in the concentration range 0.01-10 mg L(-1) using hot electron induced electrochemiluminescence (HECL) with devices combining both working and counter electrodes and sample confinement on a single chip. The sample area on the electrodes was defined by a hydrophobic ring, which enabled dispensing the reagents and the analyte directly on the electrode. Immunoassay of CRP by HECL using integrated electrodes is a good candidate for a high-sensitivity point-of-care CRP-test, because the concentration range is suitable, miniaturisation of the measurement system has been demonstrated and the assay method with integrated electrodes is easy to use. High-sensitivity CRP tests can be used to monitor the current state of cardiovascular disease and also to predict future cardiovascular problems in apparently healthy people.
authors.affiliation: Laboratory of Analytical Chemistry, Aalto University School of Chemical Technology, P.O. Box 16100, FI-00076 Aalto, Finland. tiina.ylinen-hinkka@aalto.fi
authors.forename: T
authors.initials: T
authors.lastname: Ylinen-Hinkka
authors.forename: A J
authors.initials: AJ
authors.lastname: Niskanen
authors.forename: S
authors.initials: S
authors.lastname: Franssila
authors.forename: S
authors.initials: S
authors.lastname: Kulmala
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods; D004583:Electrons; D005455:Fluorescent Antibody Technique; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008839:Microelectrodes; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity
chemical_list: D002097:C-Reactive Protein