title: Fritillaria ussuriensis extract inhibits the production of inflammatory cytokine and MAPKs in mast cells.
abstract: Fritillaria ussuriensis (FU, derived from the bulbs of various species of the genus Fritillaria, including Fritillaria thunbergii Miq.) is used in herbal medicine to treat conditions such as eczema, skin burns, and frostbite. In this study, we investigated the mechanism of the anti-allergy effect of FU. FU extract (80 mg/kg), orally administered to Sprague-Dawley (SD) rats, significantly inhibited the passive cutaneous anaphylaxis (PCA) reaction. It inhibited the compound 48/80-induced release of histamine from rat peritoneal mast cells in a concentration-dependent manner. Significant inhibitory effects of the FU extract on IL-6, IL-8, and TNF-α (1, 10, and 100 µg/mL) were observed in HMC-1 cells. Treatment with FU attenuated PMA plus A23187-induced phosphorylation of all three MAPKs, especially at concentrations of 10 and 100 µg/mL. Further, it (80 mg/kg) led to significant inhibition of mast-cell accumulation in ear tissue at the chronic phase. These results indicate that it inhibits allergic reactions.
authors.affiliation: Department of Anatomy, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea.
authors.forename: Ik-Hyun
authors.initials: IH
authors.lastname: Cho
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Lee
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Na Young
authors.initials: NY
authors.lastname: Han
authors.forename: Kyu Won
authors.initials: KW
authors.lastname: Shin
authors.forename: Youngjoo
authors.initials: Y
authors.lastname: Sohn
authors.forename: Hyuk-Sang
authors.initials: HS
authors.lastname: Jung
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000001:Calcimycin / Q000494:pharmacology; D031410:Fritillaria / Q000737:chemistry*; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects* / Q000276:immunology; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D018926:Anti-Allergic Agents; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D020929:Mitogen-Activated Protein Kinase Kinases; D013755:Tetradecanoylphorbol Acetate