title: Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines.
abstract: SlyA protein plays a key role in virulence in Salmonella enterica. In this study, we evaluated the ability of the slyA mutant strain of S. enterica serovar Choleraesuis (S. choleraesuis) to protect against swine salmonellosis. Using a murine model infected with S. enterica serovar Typhimurium (S. typhimurium), we showed that the Salmonella strain with a deletion of slyA could be used as a highly immunogenic, effective and safe vaccine in mice. Based on these data, a slyA mutant of S. enterica serovar Choleraesuis strain RF-1 was constructed, and the ability of this mutant to protect immunized pigs from S. choleraesuis infection was examined. As with the S. typhimurium slyA mutant, immunization of pigs with the S. choleraesuis slyA mutant strain provided significant protection against subsequent challenge by the wild-type RF-1. These results demonstrate that SlyA is a potential target in the development of a novel live attenuated vaccine against S. enterica.
authors.affiliation: Department of Microbiology, School of Pharmacy, Kitasato University, Minato-ku, Tokyo 108-8641, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Haneda
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okada
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: Masami
authors.initials: M
authors.lastname: Takagi
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kurotaki
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miki
authors.forename: Setsuo
authors.initials: S
authors.lastname: Arai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Danbara
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D056724:Immunity, Humoral; D016898:Interferon-alpha / Q000032:analysis / Q000276:immunology; D015847:Interleukin-4 / Q000032:analysis / Q000276:immunology; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D010581:Peyer's Patches / Q000382:microbiology / Q000473:pathology; D012480:Salmonella Infections / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D013552:Swine; D014157:Transcription Factors / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D016898:Interferon-alpha; D022562:Salmonella Vaccines; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated; C056785:salmolysin; D015847:Interleukin-4