title: Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41.
abstract: A new agarase, AgaA(CN41), cloned from Vibrio sp. strain CN41, consists of 990 amino acids, with only 49% amino acid sequence identity with known β-agarases. AgaA(CN41) belongs to the GH50 (glycoside hydrolase 50) family but yields neoagarotetraose as the end product. AgaA(CN41) was expressed and characterized.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liao
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.forename: Xia-Wei
authors.initials: XW
authors.lastname: Jiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Na
authors.initials: N
authors.lastname: Yi
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Huo
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D003001:Cloning, Molecular; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014733:Vibrio / Q000201:enzymology* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D006026:Glycoside Hydrolases; C019624:agarase