title: Efficacy of random primer-pair arrays in plant genome analysis: a case study of Cucumis (Cucurbitaceae) for identification of wild and cultivated species.
abstract: The efficacy of random primer-pair arrays compared to conventional RAPD method with a single decamer primer was evaluated using DNA from two species of Cucumis. The banding patterns of amplicons revealed enhanced utility of primer-pair arrays over conventional RAPDs, producing more bands and a higher degree of polymorphism, both at intra- and inter-specific levels. Amplification produced by both methods clearly distinguished a wild from a cultivated species of the genus Cucumis. The main advantage of the primer-pair RAPD over single-primer-based RAPD is the increase in the number of reactions and amplification products in the form of novel/unique bands with a limited number of primers. It also enables the generation of reliable amplicons with a large number of polymorphic bands, which can be linked to gene-governing traits, allowing sequence-characterized partial genome analysis.
authors.affiliation: Plant Biotechnology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, India.
authors.forename: E M
authors.initials: EM
authors.lastname: Gatphoh
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
authors.forename: K
authors.initials: K
authors.lastname: Rajkumari
authors.forename: S
authors.initials: S
authors.lastname: Rama Rao
mesh_terms: D019662:Cucurbitaceae / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D015336:Molecular Probe Techniques; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D017931:DNA Primers