title: Measuring forces between two single actin filaments during bundle formation.
abstract: Bundles of filamentous actin are dominant cytoskeletal structures, which play a crucial role in various cellular processes. As yet quantifying the fundamental interaction between two individual actin filaments forming the smallest possible bundle has not been realized. Applying holographic optical tweezers integrated with a microfluidic platform, we were able to measure the forces between two actin filaments during bundle formation. Quantitative analysis yields forces up to 0.2 pN depending on the concentration of bundling agents.
authors.affiliation: Max Planck Institute for Intelligent Systems, Department of New Materials and Biosystems, 70569 Stuttgart, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Streichfuss
authors.forename: Friedrich
authors.initials: F
authors.lastname: Erbs
authors.forename: Kai
authors.initials: K
authors.lastname: Uhrig
authors.forename: Rainer
authors.initials: R
authors.lastname: Kurre
authors.forename: Anabel E-M
authors.initials: AE
authors.lastname: Clemen
authors.forename: Christian H J
authors.initials: CH
authors.lastname: Böhm
authors.forename: Tamás
authors.initials: T
authors.lastname: Haraszti
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D000199:Actins / Q000737:chemistry*; D000255:Adenosine Triphosphate / Q000737:chemistry; D000818:Animals; D001703:Biophysics / Q000379:methods*; D003599:Cytoskeleton / Q000378:metabolism; D004573:Electrolytes; D006801:Humans; D007477:Ions; D044085:Microfluidics; D009348:Nematoda; D052898:Optical Tweezers; D055095:Optics and Photonics; D015203:Reproducibility of Results; D013314:Stress, Mechanical
chemical_list: D000199:Actins; D004573:Electrolytes; D007477:Ions; D000255:Adenosine Triphosphate