title: XRCC1 gene variants and possible links with chromosome aberrations and micronucleus in active and passive smokers.
abstract: Tobacco addiction is a major risk for diseases such as cancers, heart attack, etc. Tobacco smoke constitutes environmental toxins that are the major preventable leading cause of death worldwide. We investigated the influence of tobacco smoke on cytogenetic parameters (chromosomal aberrations and micronuclei) and the influence of XRCC1 arg399gln polymorphism on the cytogenetic parameters of the exposed subjects. The cases for this study include active and passive smokers. They were divided into three groups in accordance with duration of exposure to tobacco smoke. We observed changes in the frequency of chromosomal aberrations and micronuclei among the exposed subjects and controls. Of the three groups of exposed subjects, group III of active smokers and group III of passive smokers showed higher number of chromosomal aberrations and micronuclei when compared to controls, group I and group II of active and passive smokers. The XRCC1 arg399gln polymorphic variant gln/gln, influenced the extent of genotoxic damage in chromosomes and frequency of in micronuclei the three variants (arg/arg, arg/gln and gln/gln), gln/gln harbored significantly (P<0.05) higher number of aberrations than the arg/arg and arg/gln. In this context, the results observed in our study indicated that the single nucleotide polymorphism on XRCC1codon 399 influenced the frequencies of chromosomal aberrations and micronuclei.
authors.affiliation: Unit of Human Genetics, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamilnadu, India. genechandru@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Chandirasekar
authors.forename: K
authors.initials: K
authors.lastname: Suresh
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
authors.forename: R
authors.initials: R
authors.lastname: Venkatesan
authors.forename: B
authors.initials: B
authors.lastname: Lakshman Kumar
authors.forename: K
authors.initials: K
authors.lastname: Sasikala
mesh_terms: D016022:Case-Control Studies; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003582:Cytogenetics / Q000379:methods; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics*; D004847:Epithelial Cells / Q000502:physiology; D005260:Female; D005838:Genotype; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D009061:Mouth Mucosa / Q000166:cytology; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D012907:Smoking / Q000009:adverse effects*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D000076105:X-ray Repair Cross Complementing Protein 1
chemical_list: D004268:DNA-Binding Proteins; D014028:Tobacco Smoke Pollution; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human