title: Mechanistic insights into the translocation of full length HIV-1 Tat across lipid membranes.
abstract: The mechanism of how full length Tat (aa 1-86) crosses artificial lipid membranes was elucidated by means of fluorescence spectroscopy and fluorescence microscopy. It was shown that full length Tat (aa 1-86) neither forms pores in large unilamellar vesicles (LUVs) nor in giant unilamellar vesicles (GUVs) composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). In contrast, an N-terminally truncated Tat protein (aa 35-86) that lacks the structurally defined proline- and cysteine-rich region as well as the highly conserved tryptophan residue at position 11 generates pores in artificial POPC-membranes, through which a water-soluble dye up to a size of 10kDa can pass. By means of fluorescence microscopy, the transfer of fluorescently labeled full length Tat across POPC-bilayers was unambiguously visualized with a concomitant accumulation of the protein in the membrane interface. However, if the dye was attached to the protein, also pore formation was induced. The size of the pores was, however smaller than the protein size, i.e. the labeled protein with a mass of 11.6kDa passed the membrane, while a fluorescent dye with a mass of 10kDa was excluded from the vesicles' interior. The results demonstrate that pore formation is not the prime mechanism by which full length Tat crosses a membrane.
authors.affiliation: Institute of Organic and Biomolecular Chemistry, Tammannstr. 2, Göttingen, Germany.
authors.forename: Annegret
authors.initials: A
authors.lastname: Boll
authors.forename: Aline
authors.initials: A
authors.lastname: Jatho
authors.forename: Nadine
authors.initials: N
authors.lastname: Czudnochowski
authors.forename: Matthias
authors.initials: M
authors.lastname: Geyer
authors.forename: Claudia
authors.initials: C
authors.lastname: Steinem
mesh_terms: D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010713:Phosphatidylcholines / Q000737:chemistry; D021381:Protein Transport; D053835:Unilamellar Liposomes / Q000737:chemistry; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; D054322:tat Gene Products, Human Immunodeficiency Virus; C028694:1-palmitoyl-2-oleoylphosphatidylcholine