title: Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study.
abstract: A rapid and specific HPLC method has been developed and validated for the simultaneous determination of liensinine (CAS 2586-96-1), isoliensinine (CAS 6817-41-0) and neferine (CAS 2292-16-2) in rat plasma. The sample was prepared by a liquid-liquid extraction with diethyl ether and the recovery was above 80% from the plasma for the three compounds. Chromatographic separation was achieved with a Hypersil BDS C18 column (4.0 mm x 250 mm, particle size 5 microm). A mobile phase consisting of methanol: 0.2 M KH2PO4:0.2 M NaOH:triethylamine (71:17:12:0.002, v/v/v/v, pH 9.2-9.3) was slowly delivered at 0.8 ml/min in isocratic mode with a detection wavelength of 282 nm. The linearity of calibration curves were good (r > 0.999) in the concentration range of 0.031-2.00 microg/ ml. The lower limit of quantification can reach 0.03 microg/ml for the three compounds. The intra-day and inter-day variations estimated with QC samples were less than 8% for the three tested concentration levels. This developed method was applied in the plasma pharmacokinetic study of total bisbenzylisoquinoline alkaloids (TAL) of the lotus flower (Lian Zi Xin) following a single oral and intravenous administration of TAL in rats.
authors.affiliation: WuXi AppTec Co., Ltd, Wuxi, P R China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Libo
authors.initials: L
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Jialing
authors.initials: J
authors.lastname: Wang
authors.forename: Jizhou
authors.initials: J
authors.lastname: Xiang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D044182:Benzylisoquinolines / Q000032:analysis* / Q000493:pharmacokinetics; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D007275:Injections, Intravenous; D007546:Isoquinolines / Q000032:analysis* / Q000493:pharmacokinetics; D008297:Male; D031653:Nelumbo / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D044182:Benzylisoquinolines; D007546:Isoquinolines; D010636:Phenols; D012996:Solutions; C499779:isoliensinine; C057222:neferine; C080095:liensinine