title: In vitro prominent bone regeneration by release zinc ion from Zn-modified implant.
abstract: Zinc is one of the trace elements which induce the proliferation and the differentiation of the osteoblast. In the previous study, we found that zinc ions (Zn(2+) ion)-releasing titanium implants had excellent bone fixation using a rabbit femurs model. In this study, we isolated the Zn(2+) ions (eluted Zn(2+) ion; EZ) released from the implant surface, and evaluated the effect of EZ on the osteogenesis of human bone marrow-derived mesenchymal cells (hBMCs). In the result, it was found that the EZ stimulated cell viability, osteoblast marker gene (type I collagen, osteocalcin (OC), alkaline phosphatase (ALP) and bone sialoprotein (BSP)) expressions and calcium deposition in hBMCs.
authors.affiliation: Department of Biochemistry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yusa
authors.forename: Osamu
authors.initials: O
authors.lastname: Yamamoto
authors.forename: Masayuki
authors.initials: M
authors.lastname: Fukuda
authors.forename: Souichi
authors.initials: S
authors.lastname: Koyota
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects*; D002413:Cations, Divalent / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis; D004343:Drug Implants / Q000378:metabolism / Q000494:pharmacology; D005269:Femur; D058574:Integrin-Binding Sialoprotein / Q000096:biosynthesis; D008648:Mesoderm / Q000187:drug effects; D015675:Osteocalcin / Q000096:biosynthesis; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D024042:Collagen Type I; D004343:Drug Implants; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; D015675:Osteocalcin; D014025:Titanium; D000469:Alkaline Phosphatase; D015032:Zinc