title: Simultaneous detection of dual proteins using quantum dots coated silica nanoparticles as labels.
abstract: Simultaneous detection of multianalytes associated with a particular cancer is beneficial for disease diagnosis. Here, a facile immunosensing strategy was designed to allow simultaneous electrochemical detection of dual proteins, in a single run. CdSe and PbS water-soluble quantum dots (QDs) were prepared and coated on monodisperse silica nanoparticles as labels for proteins detection. Rabbit immunoglobulin G antigen (IgG) and carcinoembryonic antigen (CEA) were chosen as model proteins for analysis. After a typical sandwich immunoassay, CdSe and PbS QDs labels were introduced onto the Au substrates' surface, which were then dissolved and could be simultaneously monitored by square-wave-voltammetric (SWV) stripping measurements. Under selected conditions, IgG and CEA could be assayed in the ranges of 0.05-40 ng mL(-1) and 0.05-25 ng mL(-1), respectively. The proposed method possessed high sensitivity, good precision, and satisfactory reproducibility and regeneration.
authors.affiliation: School of Chemistry and Chemical Engineering, Southeast University, Sipeilou 2#, Nanjing 211189, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qian
authors.forename: Haichao
authors.initials: H
authors.lastname: Dai
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Pan
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002272:Carcinoembryonic Antigen / Q000097:blood; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D011506:Proteins; D012822:Silicon Dioxide