title: Anti-angiogenic activity evaluation of secondary metabolites from Calycolpus moritzianus leaves.
abstract: Angiogenesis is a crucial step in many pathological conditions like cancer, inflammation and metastasis formation; on these basis the search for antiangiogenic agents has widened. In order to identify new compounds able to interfere in the Vascular Endothelial Growth Factor Receptor-1 (VEGFR-1, also known as Flt-1) recognition by VEGFs family members, we screened Calycolpus moritzianus (O. Berg) Burret leaves extracts by a competitive ELISA-based assay. MeOH and CHCl3 extracts and several their fractions demonstrated to be able to prevent VEGF or PlGF interaction with Flt-1, with an inhibition about 50% at concentration of 100 microg/mL. Phytochemical and pharmacological investigation of the active fractions led to the isolation of flavonoids, and terpenes.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Lepore
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Gualtieri
authors.forename: Nicola
authors.initials: N
authors.lastname: Malafronte
authors.forename: Roberta
authors.initials: R
authors.lastname: Cotugno
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Dal Piaz
authors.forename: Letizia
authors.initials: L
authors.lastname: Ambrosio
authors.forename: Sandro
authors.initials: S
authors.lastname: De Falco
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D027822:Myrtaceae / Q000737:chemistry* / Q000378:metabolism; D000072483:Placenta Growth Factor; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D011257:Pregnancy Proteins / Q000037:antagonists & inhibitors*; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*
chemical_list: D020533:Angiogenesis Inhibitors; D005419:Flavonoids; D011257:Pregnancy Proteins; D013729:Terpenes; D042461:Vascular Endothelial Growth Factor A; D000072483:Placenta Growth Factor