title: Assessment of the cytotoxicity of aluminium oxide nanoparticles on selected mammalian cells.
abstract: The rapid development of nanotechnology raises both enthusiasm and anxiety among researchers, which is related to the safety use of the manufactured materials. Thus, the aim of this study was to investigate the effect of aluminium oxide nanoparticles on the viability of selected mammalian cells in vitro. The aluminium oxide nanoparticles were characterised using SEM and BET analyses. Based on Zeta (ζ) potential measurements and particle size distribution, the tested suspensions of aluminium oxide nanoparticles in water and nutrient solutions with or without FBS were classified as unstable. Cell viability, the degree of apoptosis induction and nanoparticles internalization into the cells were assessed after 24 h of cell exposure to Al2O3 nanoparticles. Our results confirm the ability of aluminium oxide nanoparticles to penetrate through the membranes of L929 and BJ cells. Despite this, there was no significant increase in apoptosis or decrease in cell viability observed, suggesting that aluminium oxide nanoparticles in the tested range of concentrations has no cytotoxic effects on the selected mammalian cells.
authors.affiliation: Department of Biochemistry and Biopharmaceuticals, National Medicines Institute, Chelmska 30/34, 00-725 Warsaw, Poland. eradziun@gmail.com
authors.forename: E
authors.initials: E
authors.lastname: Radziun
authors.forename: J
authors.initials: J
authors.lastname: Dudkiewicz Wilczyńska
authors.forename: I
authors.initials: I
authors.lastname: Książek
authors.forename: K
authors.initials: K
authors.lastname: Nowak
authors.forename: E L
authors.initials: EL
authors.lastname: Anuszewska
authors.forename: A
authors.initials: A
authors.lastname: Kunicki
authors.forename: A
authors.initials: A
authors.lastname: Olszyna
authors.forename: T
authors.initials: T
authors.lastname: Ząbkowski
mesh_terms: D000537:Aluminum Oxide / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D051379:Mice; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size; D013053:Spectrophotometry
chemical_list: D000537:Aluminum Oxide