title: Dissecting the molecular damage to stallion spermatozoa: the way to improve current cryopreservation protocols?
abstract: We review recent developments in the technology of freezing stallion sperm, paying special attention to the molecular lesions that spermatozoa suffer during freezing and thawing, such as osmotic stress, oxidative damage, and apoptotic changes. We also discuss the applicability of colloidal centrifugation in stallion sperm cryobiology. Increased knowledge about the molecular injuries that occur during cryopreservation may lead to improved protective techniques and thus to further improvements in fertility in the current decade.
authors.affiliation: Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura Cáceres, Spain. fjuanpvega@unex.es
authors.forename: F J
authors.initials: FJ
authors.lastname: Peña
authors.forename: B Macías
authors.initials: BM
authors.lastname: García
authors.forename: J C
authors.initials: JC
authors.lastname: Samper
authors.forename: I M
authors.initials: IM
authors.lastname: Aparicio
authors.forename: J A
authors.initials: JA
authors.lastname: Tapia
authors.forename: C Ortega
authors.initials: CO
authors.lastname: Ferrusola
mesh_terms: D000818:Animals; D017209:Apoptosis; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D006736:Horses; D008297:Male; D009997:Osmotic Pressure; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013094:Spermatozoa
chemical_list: D017382:Reactive Oxygen Species