title: [Cloning and sequence analysis of thioredoxin peroxidase gene from Taenia multiceps].
abstract: Protoscoleces of Taenia multiceps were collected from the naturally infected sheep and total RNA was extracted. Specific primers were designed according to TaHe2-D11 mRNA sequence and T. multiceps thioredoxin peroxidase gene (TmTPx) was amplified by RT-PCR. PCR products were ligated into pMD18-T vector and transformed to E. coli DH5alpha. The recombinant plasmids were identified by restriction digestion and sequencing. A 614 bp cDNA was amplified. The TmTPx open reading frame (591 bp) encoded a 196-amino acid protein with Mr 21,690, pI 7.61. Bioinformatics analysis indicated that TmTPx had a typical 2-Cys Prx conserved domain. Phylogenetic tree revealed that T. multiceps had the closest relationship to T. asiatica, followed by T. solium and T. crassiceps, E. granulosus and E. multilocularis.
authors.affiliation: Key Laboratory of Animal Paramsitology of Gansu Province, Chinese Academy of Agricultural Sciences, Lanzlhou 730046, China.
authors.forename: Yong-guang
authors.initials: YG
authors.lastname: Li
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Li
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Gai
authors.forename: Ju-xia
authors.initials: JX
authors.lastname: Yao
authors.forename: Zi-gang
authors.initials: ZG
authors.lastname: Qu
authors.forename: Wan-zhong
authors.initials: WZ
authors.lastname: Jia
authors.forename: Blaga
authors.initials: B
authors.lastname: Radu
authors.forename: Bao-quan
authors.initials: BQ
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D054464:Peroxiredoxins / Q000235:genetics*; D010957:Plasmids; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D012756:Sheep; D013621:Taenia / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018076:DNA, Complementary; D054464:Peroxiredoxins