title: Remarkable expression in the colon adenocarcinoma of Hmat-Xa, a human mannosyltransferase-like gene, that is homologous to drosophila gene GC15914.
abstract: We cloned a novel human mannosyltransferase-like gene, designated Hmat-Xa, as a gene homologous to the Drosophila GC15914 gene encoding the 9QVXN0 protein: see "Project Report for FY2002 on the 'Construction of Libraries of Human Genes Participating in Glycosylation' project" 43-45 (2003), New Energy and Industrial Technology Development Organization (NEDO), NEDO and Research Association for Biotechnology, Tokyo, Japan (in Japanese). After that, the GTDC1 gene, as reported by Zhao et al., DNA Cell Biol., 23, 183-187 (2004), was found to be the same as the Hmat-Xa gene. Domain EXFGI/L/VX(2)L/VE in the Hmat-Xa protein, also present in both human mannosyltransferase II/III and mannosyltransferase IV/V, which are involved in the synthesis of lipid-linked oligosaccharides, and some bacterial mannosyltransferases. A real-time PCR study of Hmat-Xa mRNA expression in human normal and tumor multiple tissue cDNA identified its tissue-specific expression and its remarkable expression in colon adenocarcinoma as compared to the normal counterpart. Thus the elevated expression of Hmat-Xa might serve as a candidate marker for colon adenocarcinoma.
authors.affiliation: Department of Applied Biochemistry, School of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan.
authors.forename: Nanae
authors.initials: N
authors.lastname: Shimono
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Nishimura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kamiguchi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Nishikawa
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D030541:Databases, Genetic; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli; D005333:Fetus; D015972:Gene Expression Regulation, Neoplastic; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D011994:Recombinant Proteins; C484366:GTDC1 protein, human; D016695:Glycosyltransferases