title: Modulation of inv gene expression by the OmpR two-component response regulator protein of Yersinia enterocolitica.
abstract: To elucidate the physiological meaning of OmpR-dependent expression of invasin gene (inv) inhibition in Yersinia enterocolitica, the function of the EnvZ/OmpR regulatory pathway in osmoregulation of inv expression was analyzed in detail. The osmoregulation of inv expression was found to be a multifaceted process involving both OmpR-dependent and -independent mechanisms. Analysis of inv transcription in strains lacking OmpR or EnvZ proteins indicated that kinase EnvZ is not the only regulator of OmpR phosphorylation. Using the transcriptional inv::lacZ fusion in a heterologous system (Escherichia coli) we tried to clarify the role of OmpR in the inv regulatory circuit composed of negative (H-NS) and positive (RovA) regulators of inv gene transcription. We were able to show a significant increase in inv expression in E. coli ompR background under H-NS( Ecoli )-repressed condition. Moreover, H-NS-mediated inv repression was relieved when RovA of Y. enterocolitica was expressed from a plasmid. Furthermore, we showed that RovA may activate inv expression irrespective on the presence of H-NS protein. Using this strategy we showed that OmpR of Y. enterocolitica decrease RovA-mediated inv activation.
authors.affiliation: Department of Applied Microbiology, University of Warsaw, Faculty of Biology, Institute of Microbiology, Miecznikowa 1, Warsaw, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Raczkowska
authors.forename: M
authors.initials: M
authors.lastname: Brzóstkowska
authors.forename: A
authors.initials: A
authors.lastname: Kwiatek
authors.forename: J
authors.initials: J
authors.lastname: Bielecki
authors.forename: K
authors.initials: K
authors.lastname: Brzostek
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D050939:Gene Fusion; D007763:Lac Operon / Q000235:genetics; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; C053476:invasin, Yersinia