title: Respiratory and TCA cycle activities affect S. cerevisiae lifespan, response to caloric restriction and mtDNA stability.
abstract: We studied the importance of respiratory fitness in S. cerevisiae lifespan, response to caloric restriction (CR) and mtDNA stability. Mutants harboring mtDNA instability and electron transport defects do not respond to CR, while tricarboxylic acid cycle mutants presented extended lifespans due to CR. Interestingly, mtDNA is unstable in cells lacking dihydrolipoyl dehydrogenase under CR conditions, and cells lacking aconitase under standard conditions (both enzymes are components of the TCA and mitochondrial nucleoid). Altogether, our data indicate that respiratory integrity is required for lifespan extension by CR and that mtDNA stability is regulated by nucleoid proteins in a glucose-sensitive manner.
authors.affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Cidade Universitária, Av. Prof. Lineu Prestes, 748 São Paulo, SP, Brazil 05508-000.
authors.forename: Erich B
authors.initials: EB
authors.lastname: Tahara
authors.forename: Kizzy
authors.initials: K
authors.lastname: Cezário
authors.forename: Nadja C
authors.initials: NC
authors.lastname: Souza-Pinto
authors.forename: Mario H
authors.initials: MH
authors.lastname: Barros
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Kowaltowski
mesh_terms: D002952:Citric Acid Cycle / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010101:Oxygen Consumption / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D029701:Saccharomyces cerevisiae Proteins; D008058:Dihydrolipoamide Dehydrogenase