title: Purification, crystallization and preliminary X-ray analysis of glucokinase from Streptomyces griseus in complex with glucose.
abstract: Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293 K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9 M sodium/potassium tartrate, 0.2 M NaCl and 0.1 M imidazole pH 8.1 and diffracted X-rays to 1.84 Å resolution. The crystal of SgGlkA in complex with glucose belonged to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 109.19, c = 141.18 Å. The crystal contained one molecule in the asymmetric unit.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Miyazono
authors.forename: Nobumitsu
authors.initials: N
authors.lastname: Tabei
authors.forename: Kazuya
authors.initials: K
authors.lastname: Marushima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005941:Glucokinase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013305:Streptomyces griseus / Q000201:enzymology*
chemical_list: D005941:Glucokinase; D005947:Glucose