title: Calorie restriction (CR) reduces age-dependent decline of non-homologous end joining (NHEJ) activity in rat tissues.
abstract: Even though CR has shown to enhance base excision repair (BER) and nucleotide excision repair (NER) capacities, it has not been reported whether CR can enhance non-homologous end joining (NHEJ) activity. To examine the effect of CR on NHEJ activity, ad libitum (AL)- and calorie restricted (CR)-dieted rats were used. Age-dependent decline of NHEJ activity was apparent in the lung, liver, and kidney and appeared to be slightly decreased in spleen. CR reduced age-dependent decline of NHEJ activity in all tissues, even though the extent of recovery was variable among tissues. Moreover, CR appeared to reduce age-dependent decline of XRCC4 protein level. These results suggest that CR could reduce age-dependent decline of NHEJ activity in various tissues of rats possibly through up-regulation of XRCC4.
authors.affiliation: Department of Family Medicine, Hangang Secred Heart Hospital, Hallym University Medical Center, Seoul, South Korea.
authors.forename: Jae-Eun
authors.initials: JE
authors.lastname: Lee
authors.forename: Jee-In
authors.initials: JI
authors.lastname: Heo
authors.forename: Seong-Hoon
authors.initials: SH
authors.lastname: Park
authors.forename: Jeong-Hyeon
authors.initials: JH
authors.lastname: Kim
authors.forename: Yoon-Jung
authors.initials: YJ
authors.lastname: Kho
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Kang
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Jong-Lull
authors.initials: JL
authors.lastname: Yoon
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Lee
mesh_terms: D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D031204:Caloric Restriction; D059766:DNA End-Joining Repair / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species; D013154:Spleen / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D017382:Reactive Oxygen Species; C539916:XRCC4 protein, rat