title: Microglia cell culture: a primer for the novice.
abstract: Microglial cells are the resident immune cells of the central nervous system. Progress in the recent decade has clearly established that microglial cells participate or even actively drive neurological disease. Much of our current knowledge has been generated by investigating microglial cells in cell culture. The aim of this chapter is to give the uninitiated a basic and adaptable protocol for the culturing of microglial cells. We discuss the challenges of microglial cell culture and provide a collection of tips which reflect our 25+ years of collective experience.
authors.affiliation: Department of Neurology, University of Ulm, Ulm, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Witting
authors.forename: Thomas
authors.initials: T
authors.lastname: Möller
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000166:cytology* / Q000187:drug effects; D051381:Rats
chemical_list: D008070:Lipopolysaccharides