title: Evaluation of the inhibition of mushroom tyrosinase and cellular tyrosinase activities of oxyresveratrol: comparison with mulberroside A.
abstract: The inhibitory effects of oxyresveratrol, the aglycone of mulberroside A, on mushroom and cellular tyrosinase activities and melanin synthesis were evaluated. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase, with oxyresveratrol demonstrating a greater inhibitory effect than that of mulberroside A. Oxyresveratrol and mulberroside A strongly inhibited melanin production in Streptomyces bikiniensis and exhibited dose-dependent inhibition of tyrosinase activity and inhibition of melanin synthesis in B16F10 melanoma cells. However, the compounds exhibited nearly similar inhibitory effects on the activity of cellular tyrosinase and melanin synthesis in murine melanocytes. The inhibition of melanin synthesis by mulberroside A and oxyresveratrol was involved in suppressing the expression level of melanogenic enzymes, tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2). These results indicate that the inhibition rate of mushroom tyrosinase might not provide an accurate estimate of the inhibition rate of melanin synthesis in melanocytes.
authors.affiliation: Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, South Korea.
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Kim
authors.forename: Keun-Tae
authors.initials: KT
authors.lastname: Park
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Mijin
authors.initials: M
authors.lastname: Kim
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004187:Disaccharides / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008543:Melanins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D051379:Mice; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D004187:Disaccharides; D004791:Enzyme Inhibitors; D008543:Melanins; D010936:Plant Extracts; D013267:Stilbenes; C420606:mulberroside A; C034912:puag-haad; D014442:Monophenol Monooxygenase