title: Genome of low-temperature T4-related bacteriophage vB_EcoM-VR7.
abstract: The complete genome sequence of the T4-related low-temperature Escherichia coli bacteriophage vB_EcoM-VR7 was determined. The genome sequence is 169,285 bp long, with a G+C content of 40.3%. Overall, 95% of the phage genome is coding. It encodes 293 putative protein-encoding open reading frames (ORFs) and tRNA(Met). More than half (59%) of the genomic DNA lacks significant homology with the DNA of T4, but once translated, 72% of the vB_EcoM-VR7 genome (211 ORFs) encodes protein homologues of T4 genes. Overall, 46 vB_EcoM-VR7 ORFs have no homologues in T4 but are derived from other T4-related phages, nine ORFs show similarities to bacterial or non-T4-related phage genes, and 27 ORFs are unique to vB_EcoM-VR7. This phage lacks several T4 enzymes involved in host DNA degradation; however, there is extensive representation of the DNA replication, recombination and repair enzymes as well as the viral capsid and tail structural genes.
authors.affiliation: Vilnius University Institute of Biochemistry, Mokslininkų 12, 08662, Vilnius, Lithuania, laura.kaliniene@bchi.vu.lt.
authors.forename: Laura
authors.initials: L
authors.lastname: Kaliniene
authors.forename: Vytautas
authors.initials: V
authors.lastname: Klausa
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zajančkauskaite
authors.forename: Rimas
authors.initials: R
authors.lastname: Nivinskas
authors.forename: Lidija
authors.initials: L
authors.lastname: Truncaite
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D004926:Escherichia coli / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012722:Sewage / Q000821:virology*; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012722:Sewage; D014764:Viral Proteins