title: Development of microsatellite markers for Euryale ferox (Nymphaeaceae), an endangered aquatic plant species in Japan.
abstract: OBJECTIVE
  Microsatellite markers were developed to investigate genetic diversity and genetic structure of Euryale ferox, a vulnerable aquatic plant.
  
  
  RESULTS
  Ten microsatellite loci were isolated and characterized for this species. Eight loci showed polymorphisms with two or three alleles per locus. As for the polymorphic markers, the expected and observed heterozygosities ranged from 0.00 to 0.08 and 0.17 to 0.64, respectively.
  
  
  CONCLUSIONS
  The 10 microsatellite markers described here will be useful for investigating genetic diversity, genetic structure, and gene flow between populations of E. ferox.
authors.affiliation: Graduate School of Global Environmental Studies, Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan. makinoa@kais.kyoto-u.ac.jp
authors.forename: Ayumi
authors.initials: A
authors.lastname: Imanishi
authors.forename: Shingo
authors.initials: S
authors.lastname: Kaneko
authors.forename: Yuji
authors.initials: Y
authors.lastname: Isagi
authors.forename: Junichi
authors.initials: J
authors.lastname: Imanishi
authors.forename: Yosihiro
authors.initials: Y
authors.lastname: Natuhara
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis* / Q000235:genetics; D056727:Endangered Species; D051456:Gene Flow; D005787:Gene Frequency; D015723:Gene Library; D006580:Genetic Carrier Screening / Q000379:methods; D018745:Genome, Plant; D006579:Heterozygote; D007564:Japan; D018895:Microsatellite Repeats; D030702:Nymphaeaceae / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018744:DNA, Plant