title: Serotonin 2C receptor activates a distinct population of arcuate pro-opiomelanocortin neurons via TRPC channels.
abstract: Serotonin 2C receptors (5-HT(2C)Rs) expressed by pro-opiomelanocortin (POMC) neurons of hypothalamic arcuate nucleus regulate food intake, energy homeostasis and glucose metabolism. However, the cellular mechanisms underlying the effects of 5-HT to regulate POMC neuronal activity via 5-HT(2C)Rs have not yet been identified. In the present study, we found the putative transient receptor potential C (TRPC) channels mediate the activation of a subpopulation of POMC neurons by mCPP (a 5-HT(2C)R agonist). Interestingly, mCPP-activated POMC neurons were found to be a distinct population from those activated by leptin. Together, our data suggest that 5-HT(2C)R and leptin receptors are expressed by distinct subpopulations of arcuate POMC neurons and that both 5-HT and leptin exert their actions in POMC neurons via TRPC channels.
authors.affiliation: Division of Hypothalamic Research, Department of Internal Medicine, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
authors.forename: Jong-Woo
authors.initials: JW
authors.lastname: Sohn
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Juli E
authors.initials: JE
authors.lastname: Jones
authors.forename: Kevin
authors.initials: K
authors.lastname: Wickman
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Williams
authors.forename: Joel K
authors.initials: JK
authors.lastname: Elmquist
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000502:physiology*; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels / Q000502:physiology; D020738:Leptin / Q000494:pharmacology / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000379:methods; D010879:Piperazines / Q000494:pharmacology; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000502:physiology*; D044405:Receptor, Serotonin, 5-HT2C / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology / Q000502:physiology; D058826:Serotonin 5-HT2 Receptor Agonists / Q000494:pharmacology; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D020738:Leptin; D010879:Piperazines; D044405:Receptor, Serotonin, 5-HT2C; D058826:Serotonin 5-HT2 Receptor Agonists; D050052:TRPC Cation Channels; D012701:Serotonin; D011333:Pro-Opiomelanocortin; C015068:1-(3-chlorophenyl)piperazine