title: Glia-neuron sandwich cocultures: an in vitro approach to evaluate cell-to-cell communication in neuroinflammation and neurotoxicity.
abstract: Glia-neuron sandwich cocultures are "in vitro" cell systems suitable to evaluate cell-to-cell interactions relaying on the release of soluble factors, such as proinflammatory cytokines. This chapter presents a protocol to obtain a sandwich coculture from primary rat glial cells and hippocampal neurons. Furthermore, it provides details to evaluate the release of tumor necrosis factor-α by a biological assay and neuronal cell survival, as well as examples of practical application in the investigation of neuroinflammation and its impact on neuronal function.
authors.affiliation: Toxicology Laboratory, Department of Pharmacological Sciences, University of Milan, Milan, Italy.
authors.forename: Mariaserena
authors.initials: M
authors.lastname: Boraso
authors.forename: Barbara
authors.initials: B
authors.lastname: Viviani
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D002138:Calibration; D002450:Cell Communication; D002460:Cell Line; D002470:Cell Survival; D018920:Coculture Techniques; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D007249:Inflammation / Q000473:pathology*; D009457:Neuroglia / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D011247:Pregnancy; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha