title: Effect of an experimental oil spill on vertebral bone tissue quality in European sea bass (Dicentrarchus labrax L.).
abstract: In order to identify biomarkers of oil pollution in fish we tested the effects of an experimental Light Cycle Oil (LCO) exposure on vertebral bone of sea bass, Dicentrarchus labrax L. A total of 60 adult fish were acclimated for fifteen days, then twenty were collected as controls (Day 0) while 40 were exposed to a soluble fraction of LCO (1136 ng L(-1) of ten Polycyclic Aromatic Hydrocarbons, PAHs) for seven days. Twenty of them were sampled at the end of the exposure period and the twenty last after a recovery period of fourteen days in clean seawater. Vertebral abnormalities were counted and bone mineralization, total bone area and bone density profiles were established for several post-cranial and caudal vertebrae. In sea bass, seven days of LCO exposure did not affect the frequency and severity of the vertebral abnormalities. No significant differences were observed in bone density and bone repartition (parameters of bone area profiles) between unexposed (Day 0), exposed (D7) and decontaminated (D21) fish. In contrast, bone mineralization of the vertebrae decreased in contaminated sea bass, but in a reversible way, which confirms a previous study in trout showing that this parameter is an early stress indicator. Our results suggest that vertebral bone mineralization could be used as a biomarker of PAH pollution in sea bass. It would be interesting to check this new biomarker in other teleost species exposed to various xenobiotics.
authors.affiliation: Evolution et Développement du Squelette, UMR7138, Université Pierre & Marie Curie, 7 quai St-Bernard, 75252 Paris cedex 05, France. morgane.danion@anses.fr
authors.forename: Morgane
authors.initials: M
authors.lastname: Danion
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Deschamps
authors.forename: Hélène
authors.initials: H
authors.lastname: Thomas-Guyon
authors.forename: Anne
authors.initials: A
authors.lastname: Bado-Nilles
authors.forename: Stéphane
authors.initials: S
authors.lastname: Le Floch
authors.forename: Claire
authors.initials: C
authors.lastname: Quentel
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Sire
mesh_terms: D000818:Animals; D001492:Bass / Q000002:abnormalities*; D015415:Biomarkers / Q000032:analysis; D015519:Bone Density / Q000187:drug effects; D002113:Calcification, Physiologic / Q000187:drug effects; D059392:Petroleum Pollution / Q000009:adverse effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D012623:Seawater; D013131:Spine / Q000002:abnormalities / Q000187:drug effects*; D023382:Toxicity Tests, Acute
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons