title: Culture of human pluripotent stem cells on glass slides for high-resolution imaging.
abstract: For certain applications, particularly experiments involving high-resolution imaging, it is necessary to culture cells on glass slides or cover glasses. This chapter describes techniques for successfully growing human embryonic stem cells (hESCs) on glass surfaces under three different conditions - serum-containing, serum-free, and following single-cell dissociation. It is anticipated that these techniques will extrapolate to other types of pluripotent stem cells such as induced pluripotent stem cells (iPSCs) and embryonic germ cells (EGCs).
authors.affiliation: Harley Norris Research Tower, University of Southern California Stem Cell Core, Los Angeles, CA, USA. victorif@usc.edu
authors.forename: Victoria
authors.initials: V
authors.lastname: Fox
mesh_terms: D000143:Acids; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005898:Glass; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D059010:Single-Cell Analysis; D013242:Sterilization; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000143:Acids; D016895:Culture Media, Serum-Free