title: A naturally occurring single-residue mutation in the translocator domain of Neisseria meningitidis NhhA affects trimerization, surface localization, and adhesive capabilities.
abstract: Neisseria meningitidis NhhA (Neisseria hia/hsf homologue A) is an oligomeric outer membrane protein belonging to the family of trimeric autotransporter adhesins. NhhA mediates the interaction of N. meningitidis with human epithelial cells and components of the extracellular matrix. The recombinant protein is able to induce bactericidal antibodies and hence has also been considered a potential vaccine candidate. In this study, we analyzed the production of NhhA in a large panel of N. meningitidis strains belonging to different serogroups and clonal complexes. We found that trimeric NhhA was produced at different levels by the various strains tested. In some strains belonging to the clonal complex ST41/44, the protein is detectable only as a monomer. Sequencing of the nhhA gene and generation of complementing strains in different genetic backgrounds have proved that a single mutation (Gly to Asp) in the translocator domain affected both trimerization and surface localization of NhhA. In vitro infection assays showed that this mutation impairs meningococcal NhhA-mediated adhesion, suggesting that strains carrying the mutation may rely on different strategies or molecules to mediate interaction with host cells. Finally, we demonstrated that N. meningitidis ST41/44 strains producing the mutated form did not induce killing mediated by NhhA-specific bactericidal antibodies. Our data help to elucidate the secretion mechanisms of trimeric autotransporters and to understand the contribution of NhhA in the evolutionary process of host-Neisseria interactions. Also, they might have important implications for the evaluation of NhhA as a vaccine candidate.
authors.affiliation: Novartis Vaccines and Diagnostics, Via Fiorentina, 1, 53100 Siena, Italy.
authors.forename: Hebert
authors.initials: H
authors.lastname: Echenique-Rivera
authors.forename: Brunella
authors.initials: B
authors.lastname: Brunelli
authors.forename: Maria
authors.initials: M
authors.lastname: Scarselli
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Taddei
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Pizza
authors.forename: Beatrice
authors.initials: B
authors.lastname: Aricò
authors.forename: Davide
authors.initials: D
authors.lastname: Serruto
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001422:Bacterial Adhesion / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D021381:Protein Transport
chemical_list: D018829:Adhesins, Bacterial