title: High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex.
abstract: We have developed a two-step method based on high-resolution melting (HRM) that reliably identifies species from the Cryptococcus species complex (Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii). Our results indicate that HRM can provide a fast protocol to identify and distinguish among the main Cryptococcus species.
authors.affiliation: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2, 28220 Majadahonda (Madrid), Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Gago
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Zaragoza
authors.forename: Isabel
authors.initials: I
authors.lastname: Cuesta
authors.forename: Juan L
authors.initials: JL
authors.lastname: Rodríguez-Tudela
authors.forename: Manuel
authors.initials: M
authors.lastname: Cuenca-Estrella
authors.forename: María J
authors.initials: MJ
authors.lastname: Buitrago
mesh_terms: D019411:Clinical Laboratory Techniques / Q000379:methods*; D003453:Cryptococcosis / Q000175:diagnosis / Q000382:microbiology*; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D003455:Cryptococcus neoformans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D009172:Mycology / Q000379:methods*; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors; D044366:Transition Temperature
chemical_list: D004271:DNA, Fungal