title: [Effects of mangiferin on cytokines in rats with chronic bronchitis and expression of macrophage COX-2 in mice].
abstract: OBJECTIVE
  To explore the mechanism of anti-inflammatory effect of mangiferin.
  
  
  METHODS
  The model of chronic bronchitis in rat was established by LPS + smoke. The activity of SOD, content of MDA and NO in BALF and serum, content of TNF-alpha and IL-8 were determined. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS in mice was detected by RT-PCR.
  
  
  RESULTS
  The activity of SOD, the content of NO in BALF and serum in rat with chronic bronchitis were significantly higher with high, medium and low-dose of lg mangiferin (400,200,100 mg x kg(-1)), while the content of MDA, and the content of TNF-alpha and IL-8 in lung tissues were lower. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS was significantly reduced by mangiferin with 200,100, 50 micromol x L(-1).
  
  
  CONCLUSIONS
  The anti-inflammatory mechanism of mangiferin is to relieve inflammation by raising the activity of SOD and content of NO and reducing the content of MDA and the expression of TNF-alpha, IL-8 and COX-2 mRNA.
authors.affiliation: Guangxi Traditional Chinese Medical University, Nanning 530001, China. wq@gxtcmu.edu.cn
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Jiagang
authors.initials: J
authors.lastname: Deng
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.forename: Lancheng
authors.initials: L
authors.lastname: Xu
mesh_terms: D000818:Animals; D029481:Bronchitis, Chronic / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000276:immunology; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D044004:Xanthones / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D044004:Xanthones; C013592:mangiferin; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2