title: A customized self-assembling peptide hydrogel for dental pulp tissue engineering.
abstract: Root canal therapy is common practice in dentistry. During this procedure, the inflamed or necrotic dental pulp is removed and replaced with a synthetic material. However, recent research provides evidence that engineering of dental pulp and dentin is possible by using biologically driven approaches. As tissue engineering strategies hold the promise to soon supersede conventional root canal treatment, there is a need for customized scaffolds for stem cell delivery or recruitment. We hypothesize that the incorporation of dental pulp-derived stem cells with bioactive factors into such a scaffold can promote cell proliferation, differentiation, and angiogenesis. In this study, we used a cell adhesive, enzyme-cleavable hydrogel made from self-assembling peptide nanofibers to encapsulate dental pulp stem cells. The growth factors (GFs) fibroblast growth factor basic, transforming growth factor β1, and vascular endothelial growth factor were incorporated into the hydrogel via heparin binding. Release profiles were established, and the influence of GFs on cell morphology and proliferation was assessed to confirm their bioactivity after binding and subsequent release. Cell morphology and spreading in three-dimensional cultures were visualized by using cell tracker and histologic stains. Subcutaneous transplantation of the hydrogel within dentin cylinders into immunocompromised mice led to the formation of a vascularized soft connective tissue similar to dental pulp. These data support the use of this novel biomaterial as a highly promising candidate for future treatment concepts in regenerative endodontics.
authors.affiliation: Department of Restorative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany.
authors.forename: Kerstin M
authors.initials: KM
authors.lastname: Galler
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hartgerink
authors.forename: Adriana C
authors.initials: AC
authors.lastname: Cavender
authors.forename: Gottfried
authors.initials: G
authors.lastname: Schmalz
authors.forename: Rena N
authors.initials: RN
authors.lastname: D'Souza
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D057139:Nanofibers / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D040521:Subcutaneous Tissue / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003470:Culture Media; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate