title: Chemoenzymatic synthesis of N-acetyl-D-neuraminic acid from N-acetyl-D-glucosamine by using the spore surface-displayed N-acetyl-D-neuraminic acid aldolase.
abstract: Chemoenzymatic synthesis of N-acetyl-d-neuraminic acid from N-acetyl-d-glucosamine using the spore surface-displayed N-acetyl-d-neuraminic acid aldolase at a high concentration (53.9 g liter(-1)) was achieved in this study. Thus, displaying a target enzyme on the surface of spores might be an alternative for integration of biocatalytic conversion into chemical synthesis.
authors.affiliation: State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Xu
authors.forename: Xifeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Che
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qiu
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D015870:Gene Expression; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis*; D030781:Organisms, Genetically Modified; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013171:Spores, Bacterial / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000446:Aldehyde-Lyases; C021858:neuraminic acid aldolase; D019158:N-Acetylneuraminic Acid; D000117:Acetylglucosamine