title: Kinetic behaviour of free lipase and mica-based immobilized lipase catalyzing the synthesis of sugar esters.
abstract: The utilization of natural mica as a biocatalyst support in kinetic investigations is first described in this study. The formation of lactose caprate from lactose sugar and capric acid, using free lipase (free-CRL) and lipase immobilized on nanoporous mica (NER-CRL) as a biocatalyst, was evaluated through a kinetic study. The apparent kinetic parameters, K(m) and V(max), were determined by means of the Michaelis-Menten kinetic model. The Ping-Pong Bi-Bi mechanism with single substrate inhibition was adopted as it best explains the experimental findings. The kinetic results show lower K(m) values with NER-CRL than with free-CRL, indicating the higher affinity of NER-CRL towards both substrates at the maximum reaction velocity (V(max,app)>V(max)). The kinetic parameters deduced from this model were used to simulate reaction rate data which were in close agreement with the experimental values.
authors.affiliation: Department of Chemistry, Faculty of Science, Universiti Putra Malaysia.
authors.forename: Uswatun Hasanah
authors.initials: UH
authors.lastname: Zaidan
authors.forename: Mohd Basyaruddin
authors.initials: MB
authors.lastname: Abdul Rahman
authors.forename: Siti Salhah
authors.initials: SS
authors.lastname: Othman
authors.forename: Mahiran
authors.initials: M
authors.lastname: Basri
authors.forename: Emilia
authors.initials: E
authors.lastname: Abdulmalek
authors.forename: Raja Noor Zaliha Raja Abd
authors.initials: RN
authors.lastname: Rahman
authors.forename: Abu Bakar
authors.initials: AB
authors.lastname: Salleh
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry; D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D001709:Biotechnology / Q000379:methods*; D003432:Cross-Linking Reagents; D003652:Decanoic Acids / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004952:Esters / Q000378:metabolism*; D007700:Kinetics; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000201:enzymology*; D007785:Lactose / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D015233:Models, Statistical; D049329:Nanostructures / Q000737:chemistry
chemical_list: D000538:Aluminum Silicates; D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D003652:Decanoic Acids; D004800:Enzymes, Immobilized; D004952:Esters; C031071:decanoic acid; D008049:Lipase; D007785:Lactose; C011934:mica