title: HarpinPss-mediated enhancement in growth and biological control of late leaf spot in groundnut by a chlorothalonil-tolerant Bacillus thuringiensis SFC24.
abstract: Chemical and biological approaches have been adopted to increase the growth and yield of crops and reduce loss due to diseases. We have adopted an integrated approach, where both direct antagonism and induced resistance were combined to reduce the incidence of late leaf spot (LLS) disease in groundnut caused by Phaeoisariopsis personata. Chitinolytic chlorothalonil-tolerant soil bacterium Bacillus thuringiensis SFC24 (Bt SFC24) was manipulated in vitro to express secretable form of elicitor protein harpin(Pss) of Pseudomonas syringae pv. syringae. Severity of the LLS decreased by 65% when the leaves were sprayed with B. thuringiensis expressing harpin(Pss) (Bt-pss). As seed treatment, there was an increase in growth of groundnut. Bt and Bt-pss accounted to 13% and 36% increase in shoot length. Expression of a secretable form of harpin(Pss) thus improved the ability of B. thuringiensis SFC24 to promote growth and control LLS in groundnut. In this new approach a chlorothalonil-tolerant chitinolytic bacterium was genetically engineered to secrete elicitor harpin(Pss) for dual benefit of growth promotion and disease control.
authors.affiliation: Department of Plant Sciences, University of Hyderabad, Hyderabad 500046, India.
authors.forename: Kondreddy
authors.initials: K
authors.lastname: Anil
authors.forename: Appa Rao
authors.initials: AR
authors.lastname: Podile
mesh_terms: D010367:Arachis / Q000254:growth & development / Q000382:microbiology*; D001203:Ascomycota / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D001413:Bacillus thuringiensis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D024881:Drug Resistance, Bacterial; D005659:Fungicides, Industrial / Q000378:metabolism*; D015870:Gene Expression; D009570:Nitriles / Q000378:metabolism*; D030781:Organisms, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D010572:Pest Control, Biological / Q000379:methods; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D018515:Plant Leaves / Q000254:growth & development / Q000382:microbiology; D018520:Plant Shoots / Q000254:growth & development; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005659:Fungicides, Industrial; D009570:Nitriles; D011994:Recombinant Proteins; C087295:HrpZ protein, Pseudomonas syringae; C005806:tetrachloroisophthalonitrile