title: Functional cure of SIVagm infection in rhesus macaques results in complete recovery of CD4+ T cells and is reverted by CD8+ cell depletion.
abstract: Understanding the mechanism of infection control in elite controllers (EC) may shed light on the correlates of control of disease progression in HIV infection. However, limitations have prevented a clear understanding of the mechanisms of elite controlled infection, as these studies can only be performed at randomly selected late time points in infection, after control is achieved, and the access to tissues is limited. We report that SIVagm infection is elite-controlled in rhesus macaques (RMs) and therefore can be used as an animal model for EC HIV infection. A robust acute infection, with high levels of viral replication and dramatic mucosal CD4(+) T cell depletion, similar to pathogenic HIV-1/SIV infections of humans and RMs, was followed by complete and durable control of SIVagm replication, defined as: undetectable VLs in blood and tissues beginning 72 to 90 days postinoculation (pi) and continuing at least 4 years; seroreversion; progressive recovery of mucosal CD4(+) T cells, with complete recovery by 4 years pi; normal levels of T cell immune activation, proliferation, and apoptosis; and no disease progression. This "functional cure" of SIVagm infection in RMs could be reverted after 4 years of control of infection by depleting CD8 cells, which resulted in transient rebounds of VLs, thus suggesting that control may be at least in part immune mediated. Viral control was independent of MHC, partial APOBEC restriction was not involved in SIVagm control in RMs and Trim5 genotypes did not impact viral replication. This new animal model of EC lentiviral infection, in which complete control can be predicted in all cases, permits research on the early events of infection in blood and tissues, before the defining characteristics of EC are evident and when host factors are actively driving the infection towards the EC status.
authors.affiliation: Division of Comparative Pathology, Tulane National Primate Research Center, Covington, Louisiana, United States of America.
authors.forename: Ivona
authors.initials: I
authors.lastname: Pandrea
authors.forename: Thaidra
authors.initials: T
authors.lastname: Gaufin
authors.forename: Rajeev
authors.initials: R
authors.lastname: Gautam
authors.forename: Jan
authors.initials: J
authors.lastname: Kristoff
authors.forename: Daniel
authors.initials: D
authors.lastname: Mandell
authors.forename: David
authors.initials: D
authors.lastname: Montefiori
authors.forename: Brandon F
authors.initials: BF
authors.lastname: Keele
authors.forename: Ruy M
authors.initials: RM
authors.lastname: Ribeiro
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Veazey
authors.forename: Cristian
authors.initials: C
authors.lastname: Apetrei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000378:metabolism; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D013997:Time Factors; D014779:Virus Replication / Q000276:immunology*