title: Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum.
abstract: Double isotope dilution surface-enhanced Raman scattering (double IDSERS) is qualified as a method for accurate and precise determination of biomarkers in human blood serum. Providing a full evaluation of the measurement uncertainty as well as traceability to a reference material sets the procedure in line with the requirements of a primary ratio method. Data evaluation is based on a partial least squares (PLS) model, whose prediction ability is validated from quantifying the uric acid concentration in both an artificial reference solution and a real human blood serum sample. With the proposed approach, the uric acid serum concentration can be determined with an uncertainty of 1.6% at a confidence level of 95%.
authors.affiliation: Physikalisch-Technische Bundesanstalt (PTB), Bundesallee 100, 38116 Braunschweig, Germany. Sabine.Zakel@ptb.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Zakel
authors.forename: Olaf
authors.initials: O
authors.lastname: Rienitz
authors.forename: Bernd
authors.initials: B
authors.lastname: Güttler
authors.forename: Rainer
authors.initials: R
authors.lastname: Stosch
mesh_terms: D015415:Biomarkers / Q000097:blood; D006801:Humans; D016018:Least-Squares Analysis; D011865:Radioisotope Dilution Technique; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman; D014527:Uric Acid / Q000097:blood*
chemical_list: D015415:Biomarkers; D014527:Uric Acid