title: Enhanced chondrogenic marker expression of human mesenchymal stem cells by interaction with both TGF-β3 and hyaluronic acid.
abstract: This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF-β3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes with or without TGF-β3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage-specific markers using real-time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA-coated dishes with TGF-β3 supplementation revealed a prominent increase in collagen type II, aggrecan, and Sox9. When hMSCs were cultured without TGF-β3 supplementation, only hMSCs cultured on HA-coated dishes showed prominent expression of the cartilage-specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell-adhesion matrix and a soluble growth factor.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Republic of Korea.
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Jeong-Yi
authors.initials: JY
authors.lastname: Jeon
authors.forename: Wan-Geun
authors.initials: WG
authors.lastname: La
authors.forename: Jun Yeup
authors.initials: JY
authors.lastname: Seong
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Hwang
authors.forename: Seong Eon
authors.initials: SE
authors.lastname: Ryu
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism; D002478:Cells, Cultured; D020219:Chondrogenesis; D024043:Collagen Type II / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D055758:SOX9 Transcription Factor / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology*
chemical_list: D053674:Aggrecans; D015415:Biomarkers; D024043:Collagen Type II; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D053782:Transforming Growth Factor beta3; D006820:Hyaluronic Acid