title: Multimodal biophotonic workstation for live cell analysis.
abstract: A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.
authors.affiliation: Institute of Applied Physics, University of Muenster, Corrensstraße 2/4 Muenster 48149, Germany. michael.esseling@uni-muenster.de
authors.forename: Michael
authors.initials: M
authors.lastname: Esseling
authors.forename: Björn
authors.initials: B
authors.lastname: Kemper
authors.forename: Maciej
authors.initials: M
authors.lastname: Antkowiak
authors.forename: David J
authors.initials: DJ
authors.lastname: Stevenson
authors.forename: Lionel
authors.initials: L
authors.lastname: Chaudet
authors.forename: Mark A A
authors.initials: MA
authors.lastname: Neil
authors.forename: Paul W
authors.initials: PW
authors.lastname: French
authors.forename: Gert
authors.initials: G
authors.lastname: von Bally
authors.forename: Kishan
authors.initials: K
authors.lastname: Dholakia
authors.forename: Cornelia
authors.initials: C
authors.lastname: Denz
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D002468:Cell Physiological Phenomena; D006224:Cricetinae; D003412:Cricetulus; D004867:Equipment Design; D006696:Holography / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D012666:Semiconductors