title: Determination of bisphenol A with effective pretreatment medium using automated column-switching HPLC with fluorescence detection.
abstract: A practical way for reducing contaminants, such as humic acids, and solving column-clogging problem in environmental water analysis with liquid chromatography is proposed. Detection interference by contamination is one of the most important issues of the environmental analyses. Moreover, due to the recent smaller diameter and fine particle size of an analytical column for HPLC system, a column-clogging problem is another practical difficulty as well. We found it possible to solve these problems by employing column-switching HPLC, which consists of a pretreatment column containing surface-modified polymer particles and flow changeover valves for cleaning the remaining matrices in the pretreatment column prior to analysis. This method was successfully applied to actual HPLC-fluorescence detection of bisphenol A. Limit of detection (LOD) in real sample was <0.7 ng/L. Repeatability was around 1.4% and recovery was around 97% or more. A particular pressure increase was not observed in 150 repeated analyses of real river water samples.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, Sendai, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanigawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Watabe
authors.forename: Takuya
authors.initials: T
authors.lastname: Kubo
authors.forename: Ken
authors.initials: K
authors.lastname: Hosoya
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000295:instrumentation / Q000379:methods*; D001559:Benzhydryl Compounds; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis*; D005453:Fluorescence; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D045483:Rivers / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds