title: Chemical composition and antioxidant activities of extracts from Apocyni Veneti Folium.
abstract: An expeditious and effective HPLC-UV method has been developed for the simultaneous determination of seven major flavonoids in Apocyni Veneti Folium (AVF) extract. The chemical profile of seven flavonoids, including quercetin-3-O-β-D-glc(2 → 1)-β-D-glucoside, rutin, isoquercetin, kaempferol-3-O-β-D-glucoside, quercetin-3-O-(6″-O-malonyl)-β-D-glucoside, quercetin and kaempferol was acquired by HPLC-UV. The analysis was performed on a Diamosil C18 analytical column with a gradient solvent system of acetonitrile-0.1% aqueous acetic acid. Full validation of the method was carried out (linearity, reproducibility, repeatability, accuracy and limit of detection). The results indicated that the contents of investigated flavonoids in Apocyni Veneti Folium varied significantly from habitat to habitat, with contents ranging from 0.01 to 5.57 mg g⁻¹. The antioxidant activity results demonstrate that the seven flavonoids showed great efficiency in scavenging DPPH radicals. The high content of flavonoid components of AVF could be responsible for its high antioxidant activity. This study provides powerful evidence for the relationship between the chemical ingredients of and bioactivity in AVF.
authors.affiliation: School of Chemical Engineering, Northwest University, Xi'an , Shaanxi 710069, China.
authors.forename: Sui-Hong
authors.initials: SH
authors.lastname: Yan
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Hai-Song
authors.initials: HS
authors.lastname: Zeng
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Sun
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D029062:Apocynaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts