title: The fat body transcriptomes of the yellow fever mosquito Aedes aegypti, pre- and post- blood meal.
abstract: BACKGROUND
  The fat body is the main organ of intermediary metabolism in insects and the principal source of hemolymph proteins. As part of our ongoing efforts to understand mosquito fat body physiology and to identify novel targets for insect control, we have conducted a transcriptome analysis of the fat body of Aedes aegypti before and in response to blood feeding.
  
  
  RESULTS
  We created two fat body non-normalized EST libraries, one from mosquito fat bodies non-blood fed (NBF) and another from mosquitoes 24 hrs post-blood meal (PBM). 454 pyrosequencing of the non-normalized libraries resulted in 204,578 useable reads from the NBF sample and 323,474 useable reads from the PBM sample. Alignment of reads to the existing reference Ae. aegypti transcript libraries for analysis of differential expression between NBF and PBM samples revealed 116,912 and 115,051 matches, respectively. De novo assembly of the reads from the NBF sample resulted in 15,456 contigs, and assembly of the reads from the PBM sample resulted in 15,010 contigs. Collectively, 123 novel transcripts were identified within these contigs. Prominently expressed transcripts in the NBF fat body library were represented by transcripts encoding ribosomal proteins. Thirty-five point four percent of all reads in the PBM library were represented by transcripts that encode yolk proteins. The most highly expressed were transcripts encoding members of the cathepsin b, vitellogenin, vitellogenic carboxypeptidase, and vitelline membrane protein families.
  
  
  CONCLUSIONS
  The two fat body transcriptomes were considerably different from each other in terms of transcript expression in terms of abundances of transcripts and genes expressed. They reflect the physiological shift of the pre-feeding fat body from a resting state to vitellogenic gene expression after feeding.
authors.affiliation: The Molecular Biology Program, New Mexico State University, Las Cruces, New Mexico, United States of America.
authors.forename: David P
authors.initials: DP
authors.lastname: Price
authors.forename: Vijayaraj
authors.initials: V
authors.lastname: Nagarajan
authors.forename: Alexander
authors.initials: A
authors.lastname: Churbanov
authors.forename: Peter
authors.initials: P
authors.lastname: Houde
authors.forename: Brook
authors.initials: B
authors.lastname: Milligan
authors.forename: Lisa L
authors.initials: LL
authors.lastname: Drake
authors.forename: John E
authors.initials: JE
authors.lastname: Gustafson
authors.forename: Immo A
authors.initials: IA
authors.lastname: Hansen
mesh_terms: D000330:Aedes / Q000235:genetics*; D000818:Animals; D002645:Chickens; D020451:Contig Mapping; D018076:DNA, Complementary / Q000235:genetics; D005216:Fat Body / Q000378:metabolism*; D005247:Feeding Behavior; D005786:Gene Expression Regulation; D017344:Genes, Insect / Q000235:genetics; D007109:Immunity / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D059467:Transcriptome; D015004:Yellow Fever / Q000469:parasitology*
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger