title: Role of GOLPH3 and GOLPH3L in the proliferation of human rhabdomyosarcoma.
abstract: GOLPH3 was originally identified by proteomic analyses of Golgi proteins localized in the trans-Golgi network. Recently, it was reported that GOLPH3 is up-regulated in various types of malignancies, including melanoma, colon cancer and lung cancer. However, the mechanism through which GOLPH3 is involved in the pathogenesis of rhabdomyosarcoma remains unidentified. In order to explore the function of GOLPH3 and its isoform, GOLPH3L, in the pathogenesis of rhabdomyosarcoma, we investigated the expression and knockdown effects of GOLPH3 and GOLPH3L in human rhabdomyosarcoma. Western blot analysis and real-time PCR revealed that human rhabdomyosarcoma cell lines and biopsy specimens exhibited an increased expression of GOLPH3 and GOLPH3L. GOLPH3 and GOLPH3L knockdown by siRNA prevented the proliferation of human rhabdomyosarcoma cell lines. In addition, double-knockdown of GOLPH3 and GOLPH3L also prevented the proliferation of rhabdomyosarcoma cell lines. Our findings improve the understanding of rhabdomyosarcoma pathogenesis and suggest that the knockdown of GOLPH3 or GOLPH3L may be an effective treatment for rhabdomyosarcoma.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Kunigou
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nagao
authors.forename: Naoya
authors.initials: N
authors.lastname: Kawabata
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Ishidou
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagano
authors.forename: Shingo
authors.initials: S
authors.lastname: Maeda
authors.forename: Setsuro
authors.initials: S
authors.lastname: Komiya
authors.forename: Takao
authors.initials: T
authors.lastname: Setoguchi
mesh_terms: D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006056:Golgi Apparatus / Q000502:physiology; D006801:Humans; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020033:Protein Isoforms; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D015398:Signal Transduction
chemical_list: C429024:GOLPH3 protein, human; D008565:Membrane Proteins; D020033:Protein Isoforms