title: Preparation and cell response of bio-mineralized Fe3O4 nanoparticles.
abstract: Silk fibroin (SF)-coated Fe(3)O(4) nanoparticles (NPs) with good superparamagnetism were successfully prepared via a bio-mineralization process at room temperature. Two cell tests revealed that mineralized SF-coated Fe(3)O(4) NPs presented good cytocompatibility for L929 and osteoblast cells and higher cell density after 5 d with high concentrations of SF-coated Fe(3)O(4) NPs (up to 0.5 mg/mL). These resulted from SF surface coating on NPs, nano-surface morphology and iron ion release of Fe(3)O(4) NPs. The mineralized SF-coated Fe(3)O(4) NPs could be envisioned for various bone orthopedic and therapeutic applications, in which SF-coated NPs location is controlled through an external magnetic field to promoted bone growth.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Min
authors.initials: M
authors.lastname: Deng
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zeng
authors.forename: Jianwen
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D005349:Fibroins / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010316:Particle Size; D047011:Silk / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D047011:Silk; D005349:Fibroins