title: Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.).
abstract: A linkage map of expressed sequence tag (EST)-based markers in radish (Raphanus sativus L.) was constructed using a low-cost and high-efficiency single-nucleotide polymorphism (SNP) genotyping method named multiplex polymerase chain reaction-mixed probe dot-blot analysis developed in this study. Seven hundred and forty-six SNP markers derived from EST sequences of R. sativus were assigned to nine linkage groups with a total length of 806.7 cM. By BLASTN, 726 markers were found to have homologous genes in Arabidopsis thaliana, and 72 syntenic regions, which have great potential for utilizing genomic information of the model species A. thaliana in basic and applied genetics of R. sativus, were identified. By construction and analysis of the genome structures of R. sativus based on the 24 genomic blocks within the Brassicaceae ancestral karyotype, 23 of the 24 genomic blocks were detected in the genome of R. sativus, and half of them were found to be triplicated. Comparison of the genome structure of R. sativus with those of the A, B, and C genomes of Brassica species and that of Sinapis alba L. revealed extensive chromosome homoeology among Brassiceae species, which would facilitate transfer of the genomic information from one Brassiceae species to another.
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi 981-8555, Japan.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Masako
authors.initials: M
authors.lastname: Saito
authors.forename: Sachiko
authors.initials: S
authors.lastname: Shirasawa
authors.forename: Aki
authors.initials: A
authors.lastname: Fukushima
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Ito
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Fujii
authors.forename: Sachie
authors.initials: S
authors.lastname: Kishitani
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Kitashiba
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishio
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D019607:Brassicaceae / Q000145:classification / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D020224:Expressed Sequence Tags / Q000737:chemistry*; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics*; D018745:Genome, Plant; D023281:Genomics; D015151:Immunoblotting; D060885:Multiplex Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D031224:Raphanus / Q000235:genetics; D031226:Sinapis / Q000235:genetics; D013045:Species Specificity; D026801:Synteny
chemical_list: D005819:Genetic Markers