title: [Effect of isopsoralen on the proliferation and differentiate of osteoblasts in vitro].
abstract: OBJECTIVE
  To investigate the effect of isopsoralen on proliferation, osteogenic differentiation and calcification capacity of rat calvarial osteoblasts (ROB).
  
  
  METHODS
  Segregated neonatal SD rat skull,and digestion with enzyme to obtain bone cells and cultured in MEM containing 10% FBS. Exchange the medium after three days, proceeded serial subcultivation when cells covered with 90% culture dish. Proliferation analysis was performed in 96-well plates use MTT method, isopsoralen's final concentration were 1 x 10(-4), 1 x10(-5), 1 x 10(-6), 1 x 10(-7) mmol/L. Differentiation analysis was performed in 24-well plates, the Alkaline phosphatase activity and calcium salt sediment yield and osteocalcin measured at the 4th, 8th, 12th, 16th day. At 12th day, proceeded ALP stain, and at 14th day for alizarin red staining and calcified nodule count.
  
  
  RESULTS
  When the Isopsoralen's final concentration was 1 x 10(-5) mmol/L, there was no significant effect on the ROB's proliferation, but it could promote osteogenesis. It also could raise the ALP activity and calcium salt sediment yield and osteocalcin, increase calcified tubercle amount.
  
  
  CONCLUSIONS
  When the isopsoralen final concentration is 1 x 10(-5) mmol/L, it promoted ROB differentiation and maturation. Isopsoralen may be the active ingredients of preventing anti-osteoporosis in Psoralea corylifolia.
authors.affiliation: Lanzhou General Hospital, Lanzhou 730050 China.
authors.forename: Lei-Guo
authors.initials: LG
authors.lastname: Ming
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Cheng
authors.forename: Bao-Fen
authors.initials: BF
authors.lastname: Ge
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Ma
authors.forename: Yuan-Kun
authors.initials: YK
authors.lastname: Zai
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D011564:Furocoumarins / Q000008:administration & dosage / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D015675:Osteocalcin / Q000378:metabolism; D029907:Psoralea / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000166:cytology
chemical_list: D004365:Drugs, Chinese Herbal; D011564:Furocoumarins; D015675:Osteocalcin; C011659:angelicin; D000469:Alkaline Phosphatase; D002118:Calcium