title: [Effects of polysaccharides isolated from Panax japonicus on immunosuppression mice].
abstract: OBJECTIVE
  To investigate the immunomodulatory effects of polysaccharides from Panax japonicus on immunosuppressed mice.
  
  
  METHODS
  Immunosuppressed murine model were established in Kuming mice induced by cyclophosphamide (Cy) and splenic indexes were evaluated. The proliferation activities of splenic lymphocyte were assayed by MTT and levels of serum hemolysin were confirmed by spectrophotometry. The antibody-secreting capabilities of splenocytes were assayed by quan-titative haemolysis of SRBC (QHS). The percentage of NK cells were analysed by flow cytometry (FCM).
  
  
  RESULTS
  Polysaccharides of Panax japonicus significantly increased the splenic indexes, promoted the proliferation of splenic lymphocytes, enhanced QHS reaction, elevated the production of serum hemolysin and percentages of peripheral blood NK cells in Cy-induced immunosuppressed mice.
  
  
  CONCLUSIONS
  Panax japonicus polysaccharides can recover immune function in the Cy-induced immunosuppressed mice.
authors.affiliation: Medical College of China, Three Gorges University, Yichang 443002, China. greatwall@ctgu.edu.cn
authors.forename: Chang-cheng
authors.initials: CC
authors.lastname: Zhang
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Zhao
authors.forename: Mei-jie
authors.initials: MJ
authors.lastname: Jiang
authors.forename: Hong-wu
authors.initials: HW
authors.lastname: Wang
authors.forename: Yu-min
authors.initials: YM
authors.lastname: He
authors.forename: Xiao
authors.initials: X
authors.lastname: Zeng
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001835:Body Weight; D003520:Cyclophosphamide / Q000009:adverse effects; D005260:Female; D005434:Flow Cytometry; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D027343:Rhizome / Q000737:chemistry; D013154:Spleen / Q000187:drug effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D010936:Plant Extracts; D011134:Polysaccharides; D003520:Cyclophosphamide