title: Cbl-regulated Akt and ERK signals are involved in β-elemene-induced cell apoptosis in lung cancer cells.
abstract: β-elemene is an active component of a natural plant-derived antineoplastic agent. Although the anticancer activity of β-elemene has been reported, its antitumor mechanism has not been elucidated. The aim of the present study was to investigate how Cbl-regulated Akt and ERK affect β-elemene-induced cell apoptosis in lung cancer cells. In the present study, β-elemene induced cell apoptosis in a dose-dependent manner in A549 cells. Moreover, β-elemene decreased bcl-2 expression, increased bax expression and caused the cleavage of PARP. However, β-elemene also induced the phosphorylation of Akt and ERK in a short period of time. The inhibition of Akt and ERK activation rapidly enhanced β-elemene-induced apoptosis, and the up-regulation of c-Cbl and Cbl-b expression was also detected. These results suggest that Cbl-regulated Akt and ERK signals are involved in β-elemene-induced cell apoptosis in lung cancer cells.
authors.affiliation: Department of Respiratory Medicine, The First Hospital of China Medical University, Shenyang 110001, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Qu
authors.forename: Mingfang
authors.initials: M
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Kang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Liu
authors.forename: Xuejun
authors.initials: X
authors.lastname: Hu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002867:Chromones / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D009025:Morpholines / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D002867:Chromones; D005419:Flavonoids; D009025:Morpholines; D012717:Sesquiterpenes; C445979:beta-elemene; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D050721:Proto-Oncogene Proteins c-cbl; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one