title: Anidulafungin in treatment of experimental invasive infection by Candida parapsilosis: in vitro activity, (1-->3)-beta-D-glucan and mannan serum levels, histopathological findings, and in vivo efficacy.
abstract: We have evaluated the in vitro activity of anidulafungin (AFG) against 31 strains of Candida parapsilosis sensu stricto by using broth microdilution, disk diffusion, and minimal fungicidal concentration (MFC) determination procedures. The two first methods showed a high level of activity of the drug, while MFCs were 1 to 5 dilutions higher than their corresponding MICs. To assess if MICs were predictive of in vivo outcomes, six strains representing different AFG MICs (0.12 to 2 μg/ml) were tested in a murine model of disseminated infection treated with different doses of the drug (1, 5, or 10 mg/kg of body weight). AFG was able to prolong the survival of mice infected with all the strains tested but was able to reduce the tissue burden of those mice infected only with the strains that showed the lowest MIC (0.12 μg/ml).
authors.affiliation: Unitat de Microbiologia, Facultat de Medicina, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201 Reus, Spain.
authors.forename: Valentina
authors.initials: V
authors.lastname: Salas
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Pastor
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Emilio
authors.initials: E
authors.lastname: Mayayo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quindós
authors.forename: Alfonso J
authors.initials: AJ
authors.lastname: Carrillo
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
mesh_terms: D000077612:Anidulafungin; D000818:Animals; D000935:Antifungal Agents / Q000627:therapeutic use*; D002175:Candida / Q000187:drug effects* / Q000472:pathogenicity*; D002177:Candidiasis / Q000097:blood* / Q000188:drug therapy*; D054714:Echinocandins / Q000627:therapeutic use*; D053208:Kaplan-Meier Estimate; D008297:Male; D008351:Mannans / Q000097:blood*; D051379:Mice; D008826:Microbial Sensitivity Tests; D047071:beta-Glucans / Q000097:blood*
chemical_list: D000935:Antifungal Agents; D054714:Echinocandins; D008351:Mannans; D047071:beta-Glucans; D000077612:Anidulafungin