title: The abc1-/coq8- respiratory-deficient mutant of Schizosaccharomyces pombe suffers from glutathione underproduction and hyperaccumulates Cd2+.
abstract: The abc1(-)/coq8(-) gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative stress-sensitive phenotypes to H(2)O(2), menadione, tBuOOH, Cd(2+), and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium. Mutant NBp17 produced significantly increased amounts of O(2)•- and H(2)O(2) as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity. The Cr(VI) reduction capacity and hence the •OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species. All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated yellow CdS.
authors.affiliation: Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Gazdag
authors.forename: Stefan
authors.initials: S
authors.lastname: Fujs
authors.forename: Balázs
authors.initials: B
authors.lastname: Koszegi
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Kálmán
authors.forename: Gábor
authors.initials: G
authors.lastname: Papp
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.forename: Joseph
authors.initials: J
authors.lastname: Belágyi
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
authors.forename: Miklós
authors.initials: M
authors.lastname: Pesti
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D002104:Cadmium / Q000378:metabolism*; D017353:Gene Deletion; D005978:Glutathione / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D010641:Phenotype; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*; D014451:Ubiquinone / Q000235:genetics*
chemical_list: C099890:ABC1 protein, S pombe; D018528:ATP-Binding Cassette Transporters; D016877:Oxidants; D010545:Peroxides; D029702:Schizosaccharomyces pombe Proteins; D002104:Cadmium; D014451:Ubiquinone; C030778:ubiquinone 8; D005978:Glutathione