title: NMR cross-correlated relaxation rates reveal ion coordination sites in DNA.
abstract: In this work, a novel NMR method for the identification of preferential coordination sites between physiologically relevant counterions and nucleic acid bases is demonstrated. In this approach, the NMR cross-correlated relaxation rates between the aromatic carbon chemical shift anisotropy and the proton-carbon dipolar interaction are monitored as a function of increasing Na(+), K(+), and Mg(2+) concentrations. Increasing the counterion concentration modulates the residence times of the counterions at specific sites around the nucleic acid bases. It is demonstrated that the modulation of the counterion concentration leads to sizable variations of the cross-correlated relaxation rates, which can be used to probe the site-specific counterion coordination. In parallel, the very same measurements report on the rotational tumbling of DNA, which, as shown here, depends on the nature of the ion and its concentration. This methodology is highly sensitive and easily implemented. The method can be used to cross-validate and/or complement direct but artifact-prone experimental techniques such as X-ray diffraction, NMR analysis with substitutionary ions, and molecular dynamics simulations. The feasibility of this technique is demonstrated on the extraordinarily stable DNA mini-hairpin d(GCGAAGC).
authors.affiliation: National Centre for Biomolecular Research and CEITEC, Masaryk University, Brno, Czech Republic. fiala@ncbr.chemi.muni.cz
authors.forename: Radovan
authors.initials: R
authors.lastname: Fiala
authors.forename: Nada
authors.initials: N
authors.lastname: Spacková
authors.forename: Silvie
authors.initials: S
authors.lastname: Foldynová-Trantírková
authors.forename: Jirí
authors.initials: J
authors.lastname: Sponer
authors.forename: Vladimír
authors.initials: V
authors.lastname: Sklenár
authors.forename: Lukás
authors.initials: L
authors.lastname: Trantírek
mesh_terms: D004247:DNA / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation
chemical_list: D007477:Ions; D004247:DNA