title: Bone formation induced by growth factors embedded into the nanostructured particles.
abstract: Tissue engineering has merged with stem cell biotechnology with development of new sources of transplantable biomaterials for the treatment of bone tissue diseases. Bone defects are expected to benefit from this new biotechnology because of the low self-regenerating capacity of bone matrix secreting cells. The differentiation of stem cells to bone cells using bi-functionalized multilayered particles is presented. The functionalized particles are composed of poly-glutamic acid (PGA) and poly-L-lysine (PLL) with two bone growth factors (BMP-2 and TGFbeta1) embedded into the multilayered film. The induction of bone from these bioactive particles incubated with embryonic stem cells was demonstrated in vitro. We report the demonstration of a multilayered particle-based delivery system for inducing bone formation in vivo. This new strategy is an alternative approach for in vivo bone formation.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 977 Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Facca
authors.forename: A
authors.initials: A
authors.lastname: Ferrand
authors.forename: C
authors.initials: C
authors.lastname: Mendoza-Palomares
authors.forename: F
authors.initials: F
authors.lastname: Perrin-Schmitt
authors.forename: P
authors.initials: P
authors.lastname: Netter
authors.forename: D
authors.initials: D
authors.lastname: Mainard
authors.forename: P
authors.initials: P
authors.lastname: Liverneaux
authors.forename: N
authors.initials: N
authors.lastname: Benkirane-Jessel
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D016503:Drug Delivery Systems; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology*; D011137:Polystyrenes / Q000737:chemistry; D023822:Tissue Engineering; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D055396:Bone Morphogenetic Protein 2; D036341:Intercellular Signaling Peptides and Proteins; D011137:Polystyrenes; D053773:Transforming Growth Factor beta1