title: Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study.
abstract: We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, 2,6-diCO(2)R-BDP, for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.forename: Daihi
authors.initials: D
authors.lastname: Oushiki
authors.forename: Aoi
authors.initials: A
authors.lastname: Takeda
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyamura
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.forename: Takuya
authors.initials: T
authors.lastname: Terai
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mineno
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D019556:COS Cells; D002522:Chlorocebus aethiops; D066246:ErbB Receptors / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D056004:Molecular Dynamics Simulation; D038761:Photobleaching; D017382:Reactive Oxygen Species / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D066246:ErbB Receptors