title: Harmonin inhibits presynaptic Cav1.3 Ca²⁺ channels in mouse inner hair cells.
abstract: Harmonin is a scaffolding protein that is required for normal mechanosensory function in hair cells. We found a presynaptic association of harmonin and Ca(v)1.3 Ca(2+) channels at the mouse inner hair cell synapse, which limits channel availability through a ubiquitin-dependent pathway.
authors.affiliation: Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Frederick D
authors.initials: FD
authors.lastname: Gregory
authors.forename: Keith E
authors.initials: KE
authors.lastname: Bryan
authors.forename: Tina
authors.initials: T
authors.lastname: Pangršič
authors.forename: Irina E
authors.initials: IE
authors.lastname: Calin-Jageman
authors.forename: Tobias
authors.initials: T
authors.lastname: Moser
authors.forename: Amy
authors.initials: A
authors.lastname: Lee
mesh_terms: D000367:Age Factors; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D020746:Calcium Channels, L-Type / Q000172:deficiency / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002461:Cell Line, Transformed; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism; D007758:Ear, Inner / Q000166:cytology; D004558:Electric Stimulation; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000235:genetics; D008564:Membrane Potentials / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D054731:PDZ Domains / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000502:physiology*; D011485:Protein Binding / Q000187:drug effects; D013569:Synapses / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D025801:Ubiquitin / Q000378:metabolism
chemical_list: C485995:Cacna1d protein, mouse; D020746:Calcium Channels, L-Type; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D025801:Ubiquitin; C492262:Ush1c protein, mouse; D000429:Alcohol Oxidoreductases; C096794:C-terminal binding protein; D002118:Calcium