title: [Proteomic research on anti-atherosclerosis mechanism of curcumin in RAW264.7].
abstract: OBJECTIVE
  Two-dimensional difference gel electrophoresis and mass spectrum were used to study the anti-atherosclerosis mechanism of curcumin.
  
  
  METHODS
  The proteins from RAW264.7 cell and RAW264.7 cell treated with 25 micromol x L(-1) curcumin were labeled with Cy3 or Cy5 randomly. Each Cy3-labeled sample and Cy5-labeled sample was mixed on the same 2-D gel along with a Cy2-labeled mixture of all samples as an internal standard and run on the same gel. The gels were scanned under different wave-length light after electrophoresis. All images were analyzed by DeCyder 6.5 software, and the different proteins were identified by mass spectrum.
  
  
  RESULTS
  The expression of ATP synthesis H+ transporting, MHC class II, non-muscle myosin alkali light chain and cytochrome b5 increased in the RAW264.7 cell treated with 25 micromol x L(-1) curcumin, while the expression of phosphodiesterase 4D, elF-3, Hnrpf protein, vimentin, nucleophosminl and Ranbp 1 decreased.
  
  
  CONCLUSIONS
  The anti-atherosclerosis mechanism of curcumin is the result of enhancement of the cell inflammation, antioxidant activity and inhibition of cholesterol transport, reduce of the accumulation of intracellular cholesterol and other factors. In addition, curcumin also had the effect of anti-tumor through regulated tumor cell differentiation and apoptosis.
authors.affiliation: College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. ludezhao@126.com
authors.forename: Dezhao
authors.initials: D
authors.lastname: Lu
authors.forename: Taoqi
authors.initials: T
authors.lastname: Lin
authors.forename: Xingde
authors.initials: X
authors.lastname: Wo
authors.forename: Pinger
authors.initials: P
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism*; D002460:Cell Line; D003474:Curcumin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D015870:Gene Expression / Q000187:drug effects; D008285:Major Histocompatibility Complex / Q000502:physiology; D013058:Mass Spectrometry; D051379:Mice; D040901:Proteomics / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003474:Curcumin