title: Determination of tadalafil and N-desmethylsibutramine in health and dietary supplements using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS).
abstract: The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 µg kg(-1) and from 1.3 to 6.0 µg kg(-1), respectively. This method allows easy and fast analysis and detection of diverse adulterants.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Roh
authors.forename: Y P
authors.initials: YP
authors.lastname: Kang
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: Y
authors.initials: Y
authors.lastname: Huh
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: D
authors.initials: D
authors.lastname: Kim
authors.forename: S W
authors.initials: SW
authors.lastname: Kwon
mesh_terms: D002243:Carbolines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D003503:Cyclobutanes / Q000032:analysis* / Q000737:chemistry; D019587:Dietary Supplements; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D000068581:Tadalafil
chemical_list: D002243:Carbolines; D003503:Cyclobutanes; D000068581:Tadalafil; C058254:sibutramine