title: Molecular identification of Bartonella quintana infection using species-specific real-time PCR targeting transcriptional regulatory protein (bqtR) gene.
abstract: We describe a SYBR Green I-based real-time PCR targeting Bartonella quintana transcriptional regulatory protein (bqtR) gene, recently found as invariant gene among different B. quintana strains. Melting curve analysis allowed us to discriminate between B. quintana and Bartonella henselae amplified products. We also show its usefulness in the management of a blood culture-negative patient affected by enlarged cervical lymphonodes and long-lasting fever. B. quintana DNA detection in patient whole blood samples and blood culture bottles was confirmed by sequencing and analyzing amplified products.
authors.affiliation: Institute of Microbiology, Dept. of Medical Sciences, Magna Graecia University, Via Tommaso Campanella 115, 88100 Catanzaro, Italy. mliberto@unicz.it
authors.forename: Maria Carla
authors.initials: MC
authors.lastname: Liberto
authors.forename: Angelo G
authors.initials: AG
authors.lastname: Lamberti
authors.forename: Nadia
authors.initials: N
authors.lastname: Marascio
authors.forename: Giovanni
authors.initials: G
authors.lastname: Matera
authors.forename: Angela
authors.initials: A
authors.lastname: Quirino
authors.forename: Giorgio S
authors.initials: GS
authors.lastname: Barreca
authors.forename: Francesco
authors.initials: F
authors.lastname: Baudi
authors.forename: Alfredo
authors.initials: A
authors.lastname: Focà
mesh_terms: D015373:Bacterial Typing Techniques; D001474:Bartonella Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology; D018416:Bartonella henselae / Q000235:genetics* / Q000302:isolation & purification; D018413:Bartonella quintana / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004269:DNA, Bacterial / Q000097:blood*; D006801:Humans; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial