title: Depletion of Nrf2 enhances inflammation induced by oxyhemoglobin in cultured mice astrocytes.
abstract: Nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element pathway has been proved to be the key regulator in reducing inflammatory damage, which is involved in subarachnoid hemorrhage (SAH). Here, in a traditional in vitro SAH model, we investigated the effect of Nrf2 depletion on pro-inflammatory cytokines production. Primary cultured astrocytes from Nrf2 wild type (WT) or knockout (KO) mouse were exposed or not exposed to oxyhemoglobin (OxyHb). Then the DNA-binding activity of transcription factor nuclear factor-κB (NF-κB) was detected by EMSA. The expression of TNF-α, IL-1β, IL-6 and MMP9 were evaluated. The activity of MMP9 was measured by Gelatin zymography. After exposure to OxyHb, NF-κB was activated and the expression of downstream pro-inflammatory cytokines was up-regulated in astrocytes. And such up-regulation was much higher in KO astrocytes than in WT astrocytes, which means more aggravated inflammation in Nrf2 deficient astrocytes. These results suggest that astrocytes participate in inflammatory process after SAH and the absence of Nrf2 may induce more aggressive inflammation through activation of NF-κB pathway.
authors.affiliation: Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, 305 East Zhongshan Road, Nanjing, 210002 Jiangsu Province, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pan
authors.forename: Handong
authors.initials: H
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Mao
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.forename: Xingfen
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D016923:Cell Death; D002478:Cells, Cultured; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D053583:Interleukin-1beta; D015850:Interleukin-6 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D051379:Mice; D018345:Mice, Knockout; D051267:NF-E2-Related Factor 2 / Q000172:deficiency* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010108:Oxyhemoglobins / Q000633:toxicity*; D013345:Subarachnoid Hemorrhage / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015854:Up-Regulation
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; D010108:Oxyhemoglobins; D014409:Tumor Necrosis Factor-alpha; D020780:Matrix Metalloproteinase 9