title: Potential of human decidua stem cells for angiogenesis and neurogenesis.
abstract: OBJECTIVE
  Placenta as a fetomaternal organ is a potential source of fetal as well as maternal stem cells. This present study describes novel properties of the cells isolated from the maternal part of term placenta membrane, the decidua basalis.
  
  
  METHODS
  Colony-forming unit-fibroblast (CFU-F) frequency and immunophenotype of human decidua-derived stem cells (hDSC) was carried out using flow cytometry. Quantitative polymerase chain reaction was performed to reveal the stemness, angiogenic- and endothelial cell-associated genes expression in serial-passage hDSC. Adipogenic and osteogenic differentiation potential of passage 5 (P5) cells were determined. We also performed immunostaining of common angiogenic/endogenic (CD31 and vWF) and neurogenic markers (GFAP, NF, NSE, vimentin and nestin) on hDSC at P5.
  
  
  RESULTS
  HDSC contains high clonogenic precursor with 1:25 CFU-F frequency. Mesenchymal stem cell-associated markers CD90, CD9, CD44, CD73 and HLA ABC were highly expressed in P0 and P5 hDSC. The specific lineage markers CD117, CD45, CD34, CD31 and HLA DR DP DQ were scarcely expressed. HDSC expressed all the stem cell-associated genes and the expression was maintained until P5. Also, the cells are capable of differentiating into adipogenic and osteogenic lineage. Positive expressions of angiogenic/endogenic markers (CD31, vWF) and neurogenic markers (GFAP, NF, NSE, vimentin and nestin) were demonstrated by hDSC.
  
  
  CONCLUSIONS
  Human decidua contains stem cells with great proliferation capacity and mesenchymal properties. Expressions of angiogenic/endogenic and neurogenic markers support the conclusion that hDSC is a promising stem cell source for neurogenesis as well as angiogenesis.
authors.affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
authors.forename: Abdul-Rahman
authors.initials: AR
authors.lastname: Hayati
authors.forename: Mohd-Manzor
authors.initials: MM
authors.lastname: Nur Fariha
authors.forename: Geok-Chin
authors.initials: GC
authors.lastname: Tan
authors.forename: Ay-Eeng
authors.initials: AE
authors.lastname: Tan
authors.forename: Kienhui
authors.initials: K
authors.lastname: Chua
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D048430:Cell Shape; D003656:Decidua / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D016130:Immunophenotyping; D018919:Neovascularization, Physiologic / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D010920:Placenta / Q000033:anatomy & histology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D015415:Biomarkers