title: Modeling binge-like ethanol drinking by peri-adolescent and adult P rats.
abstract: Alcohol binge-drinking, especially among adolescents and young adults, is a serious public health concern. The present study examined ethanol binge-like drinking by peri-adolescent [postnatal days (PNDs 30-72)] and adult (PNDs 90-132) alcohol-preferring (P) rats with a drinking-in-the-dark-multiple-scheduled-access (DID-MSA) procedure used by our laboratory. Male and female P rats were provided concurrent access to 15% and 30% ethanol for three 1-h sessions across the dark cycle 5 days/week. For the 1st week, adolescent and adult female P rats consumed 3.4 and 1.6g/kg of ethanol, respectively, during the 1st hour of access, whereas for male rats the values were 3.5 and 1.1g/kg of ethanol, respectively. Adult intakes increased to ~2.0 g/kg/h and adolescent intakes decreased to ~2.5 g/kg/h across the 6 weeks of ethanol access. The daily ethanol intake of adult DID-MSA rats approximated or modestly exceeded that seen in continuous access (CA) rats or the selection criterion for P rats (≥5 g/kg/day). However, in general, the daily ethanol intake of DID-MSA peri-adolescent rats significantly exceeded that of their CA counterparts. BELs were assessed at 15-min intervals across the 3rd hour of access during the 4th week. Ethanol intake was 1.7 g/kg vs. 2.7 g/kg and BELs were 57 mg% vs. 100mg% at 15- and 60-min, respectively. Intoxication induced by DID-MSA in female P rats was assessed during the 1st vs. 4th week of ethanol access. Level of impairment did not differ between the 2 weeks (106 vs. 97 s latency to fall, 120 s criterion) and was significant (vs. naïve controls) only during the 4th week. Overall, these findings support the use of the DID-MSA procedure in rats, and underscore the presence of age- and sex-dependent effects mediating ethanol binge-like drinking in P rats.
authors.affiliation: Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, IN 46202-4887, USA. ribell@iupui.edu
authors.forename: Richard L
authors.initials: RL
authors.lastname: Bell
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: Rodd
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Smith
authors.forename: Jamie E
authors.initials: JE
authors.lastname: Toalston
authors.forename: Kelle M
authors.initials: KM
authors.lastname: Franklin
authors.forename: William J
authors.initials: WJ
authors.lastname: McBride
mesh_terms: D000367:Age Factors; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000523:psychology; D000818:Animals; D004195:Disease Models, Animal; D000431:Ethanol / Q000506:poisoning*; D005260:Female; D008297:Male; D023421:Models, Animal; D051381:Rats; D012646:Self Administration
chemical_list: D000431:Ethanol