title: Oxygen convective uptakes in gas exchange cycles in early diapause pupae of Pieris brassicae.
abstract: Oxygen convective uptakes in gas exchange cycles were directly recorded in early diapause pupae of Pieris brassicae L. (Lepidoptera; Pieridae) by means of O2 coulometric respirometry. This method was combined with flow-through CO2 respirometry, the two systems being switchable one to the other. During recording with both systems, measurements were also taken with infrared actography. The pupae displayed short discontinuous gas exchange cycles lasting 40-70 min. No true C phase was found by flow-through measurements; instead, flutter opening of the spiracles with discrete convective O2 uptakes began shortly after the O phase whereas CO2 release was suppressed by the inward directed passive suction ventilation. The F phase was characterized by a series of small CO2 bursts (flutter events). Between these bursts, novel sub-phase `miniflutter' was observed, which consisted of six to 10 miniature inspirations without any CO2 emission. During the flow-through measurements, oxygen convective uptakes were indirectly recorded by the infrared actograph as sudden extensions (lengthening) of the abdominal segments at each spiracular microopening.
authors.affiliation: Estonian University of Life Sciences, Institute Agricultural and Environmental Sciences, Department of Plant Protection, Kreutzwaldi 1, Tartu 51014, Estonia. katrin.jogar@emu.ee
authors.forename: Katrin
authors.initials: K
authors.lastname: Jõgar
authors.forename: Aare
authors.initials: A
authors.lastname: Kuusik
authors.forename: Angela
authors.initials: A
authors.lastname: Ploomi
authors.forename: Luule
authors.initials: L
authors.lastname: Metspalu
authors.forename: Ingrid
authors.initials: I
authors.lastname: Williams
authors.forename: Külli
authors.initials: K
authors.lastname: Hiiesaar
authors.forename: Irja
authors.initials: I
authors.lastname: Kivimägi
authors.forename: Marika
authors.initials: M
authors.lastname: Mänd
authors.forename: Tea
authors.initials: T
authors.lastname: Tasa
authors.forename: Anne
authors.initials: A
authors.lastname: Luik
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D007915:Lepidoptera / Q000378:metabolism*; D008675:Metamorphosis, Biological; D010100:Oxygen / Q000378:metabolism*; D011679:Pupa / Q000378:metabolism
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen