title: Effect of the modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine on European and North American PRRSV shedding in semen from infected boars.
abstract: The objective of the present study was to compare the effects of the modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine (Ingelvac PRRS MLV; Boehringer Ingelheim Animal Health, St. Joseph, MO) on European and North American PRRSV shedding in the semen of experimentally infected boars. The boars were randomly divided into six groups. Vaccinated boars shed the North American PRRSV at the rate of 10(0.1) to 10(1.0) viral genome copies per ml and 3.63 to 10(1.1) 50% tissue culture infective doses (TCID(50))/ml, respectively, in semen, whereas nonvaccinated boars shed the North American PRRSV at the rate of 10(0.2) to 10(4.7) viral genome copies per ml and 1.14 to 10(3.07) TCID(50)/ml, respectively, in semen. Vaccinated boars shed the European PRRSV at the rate of 10(0.1) to 10(4.57) viral genome copies per ml and 1.66 to 10(3.10) TCID(50)/ml, respectively, in semen, whereas nonvaccinated boars shed the European PRRSV at the rate of 10(0.3) to 10(5.14) viral genome copies per ml and 1.69 to 10(3.17) TCID(50)/ml, respectively, in semen. The number of genomic copies of the European PRRSV in semen samples was not significantly different between vaccinated and nonvaccinated challenged European PRRSV boars. The present study demonstrated that boar vaccination using commercial modified live PRRSV vaccine was able to decrease subsequent shedding of North American PRRSV in semen after challenge but was unable to decrease shedding of European PRRSV in semen after challenge.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Han
authors.forename: Hwi Won
authors.initials: HW
authors.lastname: Seo
authors.forename: Jeoung Hwa
authors.initials: JH
authors.lastname: Shin
authors.forename: Yeonsu
authors.initials: Y
authors.lastname: Oh
authors.forename: Ikjae
authors.initials: I
authors.lastname: Kang
authors.forename: Changhoon
authors.initials: C
authors.lastname: Park
authors.forename: Chanhee
authors.initials: C
authors.lastname: Chae
mesh_terms: D000818:Animals; D008969:Molecular Sequence Data; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D012661:Semen / Q000276:immunology / Q000821:virology*; D017422:Sequence Analysis, DNA; D013552:Swine; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D017201:Virus Shedding / Q000276:immunology*
chemical_list: D012367:RNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines