title: Winogradskyella ulvae sp. nov., an epiphyte of a Pacific seaweed, and emended descriptions of the genus Winogradskyella and Winogradskyella thalassocola, Winogradskyella echinorum, Winogradskyella exilis and Winogradskyella eximia.
abstract: A Gram-negative, facultatively anaerobic, slightly halophilic bacterium that was motile by gliding, designated KMM 6390(T), was isolated from the coastal green alga Ulva fenestrata collected from the Sea of Japan. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6390(T) was a member of the genus Winogradskyella, with Winogradskyella echinorum KMM 6211(T) as its closest relative (97.8% 16S rRNA gene sequence similarity). 16S rRNA gene sequence similarities between the isolate and other members of the genus Winogradskyella were 95.4-96.7%. DNA-DNA relatedness between the isolate and W. echinorum KMM 6211(T) was 27%. Strain KMM 6390(T) was able to grow with 0.5-4.0% NaCl and at 4-37 °C. The isolate decomposed casein, gelatin, starch and DNA and produced acid from galactose, D-glucose, lactose and maltose. The major fatty acids were iso-C(15:0), iso-C(15:1), anteiso-C(15:0) and C(15:0). The polar lipid profile comprised phosphatidylethanolamine and two unknown aminolipids. The DNA G+C content was 34.2 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic investigations, strain KMM 6390(T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella ulvae sp. nov. is proposed; the type strain is KMM 6390(T) (=KCTC 23626(T)=LMG 26444(T)). Emended descriptions of the genus Winogradskyella and of W. thalassocola, W. echinorum, W. exilis and W. eximia are also provided.
authors.affiliation: Pacific Institute of Bioorganic Chemistry of the Far-Eastern Branch of the Russian Academy of Sciences, Pr. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Nedashkovskaya
authors.affiliation: Far Eastern Federal University, Sukhanova St. 8, 690950 Vladivostok, Russia.|A.V. Zhirmunsky Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Science, Pal'chevskogo St. 17, 690032 Vladivostok, Russia.
authors.forename: Andrey D
authors.initials: AD
authors.lastname: Kukhlevskiy
authors.affiliation: Far Eastern Federal University, Sukhanova St. 8, 690950 Vladivostok, Russia.|A.V. Zhirmunsky Institute of Marine Biology of the Far-Eastern Branch of the Russian Academy of Science, Pal'chevskogo St. 17, 690032 Vladivostok, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Zhukova
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D007564:Japan; D008124:Locomotion; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D012624:Seaweed / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride