title: [Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint].
abstract: OBJECTIVE
  To establish HPLC fingerprint of Folium Pyrrosiae for identification.
  
  
  METHODS
  The HPLC method was developed with Diamonsil C18 column (250 mm x 4.6 mm, 5 microm),and a mixture liquid of acetonitrile-0.8% acetic acid solution as mobile phase in a gradient elution. HPLC fingerprints of44 samples were analyzed by similarity, cluster and principal component analysis.
  
  
  RESULTS
  The HPLC fingerprint common pattern of Pyrrosia petiolosa, Pyrrosia lingua and common pattern of Pyrrosia sheareri were set up separately. Samples from different species were classified based on the result of cluster and principal component analysis. Fingerprints of Pyrrosia sheareri and Pyrrosia lingua have high degree of similarity, but were different from Pyrrosia petiolosa, while Pyrrosia calvata and Pyrrosia assimlis were classified as adulterants with their dissimilar fingerprints.
  
  
  CONCLUSIONS
  The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Folium Pyrrosiae from different habitats and species.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Yi-xuan
authors.initials: YX
authors.lastname: Zhang
authors.forename: Kai-tong
authors.initials: KT
authors.lastname: Li
authors.forename: Qiu-yue
authors.initials: QY
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D029621:Polypodiaceae / Q000737:chemistry* / Q000145:classification / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D012503:Saponins; D002726:Chlorogenic Acid