title: Magnetic resonance imaging-guided microdialysis cannula implantation in a spontaneous high-grade glioma murine model.
abstract: Cerebral microdialysis is used to study anticancer drug penetration in the central nervous system (CNS) and brain tumors in animal models. Genetically engineered murine models (GEMMs) have been recently used to study many aspects of CNS tumors since they represent a more relevant model than orthotopic brain tumor xenograft models. However, it is challenging to implant microdialysis cannula in these animals because T2-weighted magnetic resonance imaging (MRI) does not show the reference point (bregma) traditionally used to obtain stereotactic coordinates. Thus, an alternative reference point that can be visualized on MRI images is needed. In this study, a novel reference point, identified as the intersection between the olfactory bulb/frontal lobe border and the midline between cerebral hemispheres on T2-weighted MRI images, was used to calculate anterior-posterior and medial-lateral coordinates of brain tumors in a GEMM. This point overlies a visible crossover between the rostral rhinal vein and the midline suture on the mouse skull, allowing for the conversion of the MRI coordinates into surgical stereotactic coordinates. Postmortem MRI and histological examination confirmed accurate probe placement. This procedure will facilitate the accurate and precise implantation of microdialysis probes for the study of anticancer drug penetration in brain tumors of GEMMs.
authors.affiliation: Department of Pharmaceutical Sciences, St. Jude Children's Research Hospital, Memphis, Tennessee 38105; Integrated Program in Biomedical Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Elmeliegy
authors.forename: Angel M
authors.initials: AM
authors.lastname: Carcaboso
authors.forename: Lionel M
authors.initials: LM
authors.lastname: L Chow
authors.forename: Ziwei M
authors.initials: ZM
authors.lastname: Zhang
authors.forename: Christopher
authors.initials: C
authors.lastname: Calabrese
authors.forename: Stacy L
authors.initials: SL
authors.lastname: Throm
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Baker
authors.forename: Clinton F
authors.initials: CF
authors.lastname: Stewart
mesh_terms: D059925:Anatomic Landmarks; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002404:Catheterization / Q000295:instrumentation*; D002408:Catheters, Indwelling; D004195:Disease Models, Animal; D004867:Equipment Design; D005910:Glioma / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018810:Magnetic Resonance Angiography; D053783:Magnetic Resonance Imaging, Interventional; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D017551:Microdialysis / Q000295:instrumentation*; D060787:Neoplasm Grading
chemical_list: D000970:Antineoplastic Agents