title: Nicotinic acetylcholine receptor β2 subunits in the medial prefrontal cortex control attention.
abstract: More than one-third of all people are estimated to experience mild to severe cognitive impairment as they age. Acetylcholine (ACh) levels in the brain diminish with aging, and nicotinic ACh receptor (nAChR) stimulation is known to enhance cognitive performance. The prefrontal cortex (PFC) is involved in a range of cognitive functions and is thought to mediate attentional focus. We found that mice carrying nAChR β2-subunit deletions have impaired attention performance. Efficient lentiviral vector-mediated reexpression of functional β2-subunit-containing nAChRs in PFC neurons of the prelimbic area (PrL) completely restored the attentional deficit but did not affect impulsive and motivational behavior. Our findings show that β2-subunit expression in the PrL PFC is sufficient for endogenous nAChR-mediated cholinergic regulation of attentional performance.
authors.affiliation: Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research (CNCR), Neuroscience Campus Amsterdam, VU University, 1081 HV Amsterdam, Netherlands.
authors.forename: Karine
authors.initials: K
authors.lastname: Guillem
authors.forename: Bernard
authors.initials: B
authors.lastname: Bloem
authors.forename: Rogier B
authors.initials: RB
authors.lastname: Poorthuis
authors.forename: Maarten
authors.initials: M
authors.lastname: Loos
authors.forename: August B
authors.initials: AB
authors.lastname: Smit
authors.forename: Uwe
authors.initials: U
authors.lastname: Maskos
authors.forename: Sabine
authors.initials: S
authors.lastname: Spijker
authors.forename: Huibert D
authors.initials: HD
authors.lastname: Mansvelder
mesh_terms: D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001288:Attention; D001522:Behavior, Animal; D003071:Cognition; D017353:Gene Deletion; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009042:Motivation; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D017397:Prefrontal Cortex / Q000502:physiology*; D011930:Reaction Time; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D000109:Acetylcholine; D011978:Receptors, Nicotinic