title: NAD+ treatment induces delayed autophagy in Neuro2a cells partially by increasing oxidative stress.
abstract: NAD(+) plays important roles in various biological processes. In this study, we reported that treatment of NAD(+) induces delayed autophagy in Neuro2a cells. Moreover, the effects of NAD(+) on the autophagy in the cells appear to be, at least partially, mediated by oxidative stress. However, nicotinamide, a degradation product of NAD(+), does not affect the autophagy. Our experiments have further indicated that the NAD(+)-induced autophagy contributes to the NAD(+)-induced decrease in the survival of these cells. In summary, our study has provided the first evidence that NAD(+) treatment induces autophagy in cancer cells such as Neuro2a cells, which contributes to the NAD(+)-induced decrease in cancer cell survival.
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Shengtao
authors.initials: S
authors.lastname: Shi
authors.forename: Lan
authors.initials: L
authors.lastname: Min
authors.forename: Teresa
authors.initials: T
authors.lastname: Wu
authors.forename: Weiliang
authors.initials: W
authors.lastname: Xia
authors.forename: Weihai
authors.initials: W
authors.lastname: Ying
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009243:NAD / Q000494:pharmacology*; D009447:Neuroblastoma; D009536:Niacinamide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C115010:Becn1 protein, mouse; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D009243:NAD; D009536:Niacinamide