title: Application of a disposable screen-printed electrode to depression diagnosis for laboratory rats based on blood serotonin detection.
abstract: No exact and digital diagnostic methods for depression have been found. In this study, we prepared a 5-HT biosensor based on screen-printed electrode and applied it to a rat depression model caused by chronic unpredictable mild stress (CUMS). During CUMS, the blood 5-HT and the depression behavior of the depressed rats and the rats treated by antidepressants were recorded. The correlation coefficient of 5-HT was 0.9966 (0 - 4 × 10(-6) M) on the sensor. Results demonstrated that 5-HT level of the depressed rats declined while the depression behavior was aggravated. Fluoxetine (20 mg/kg) and lentinan (10, 20 mg/kg) mildly elevated 5-HT level and slowly regulated the behavior. Mifepristone (20 mg/kg) and Rhizoma Coptidis water extract (10, 20, 100 mg/kg) quickly reversed 5-HT level and the depression behavior. This sensor can accurately test blood 5-HT and might be applied to rapid diagnosis for depression and evaluation of antidepressants effect.
authors.affiliation: Department of Pharmacy, South-Central University for Nationalities, Wuhan 430074, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Jiajie
authors.initials: J
authors.lastname: Hu
authors.forename: Tian
authors.initials: T
authors.lastname: Ma
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use; D015374:Biosensing Techniques; D003863:Depression / Q000097:blood / Q000188:drug therapy; D004195:Disease Models, Animal; D004209:Disposable Equipment; D004566:Electrodes; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000097:blood*
chemical_list: D000928:Antidepressive Agents; D012701:Serotonin