title: Preparation and in vitro/in vivo evaluations of dimpled poly(L-lactic acid) fibers mixed/coated with hydroxyapatite nanocrystals.
abstract: A novel hydroxyapatite (HAp)/poly(L-lactic acid) (PLLA) nanocomposite nonwoven fabric, which was coated and mixed with calcined HAp nanocrystals, and has submicron-sized dimples on its surface, was fabricated. First, HAp-mixed PLLA fabric was prepared by electrospinning a HAp nanocrystal dispersion in dichloromethane (DCM)-dissolved PLLA. It was found that most of the HAp nanocrystals were not exposed on the HAp-mixed PLLA fiber surface but covered with the PLLA matrix. A HAp-nanocrystal coating was applied onto the surface of the HAp-mixed PLLA fabric after corona discharge treatment followed by ethanol washing. The submicron-sized dimples were enlarged after the ethanol washing. After the HAp-nanocrystal coating, the HAp-mixed PLLA fabric surface was uniformly coated with the HAp nanocrystals. In vitro cell spread tests showed that the rat osteoblasts spread more on HAp-nanocrystal-coated fabrics than on non-HAp-coated fabrics. Upon covering calvarial defects, the in vivo hard tissue responses suggested earlier restoration of the defects with HAp-nanocrystal-coated fabrics than those with non-HAp-coated fabrics.
authors.affiliation: Department of Bioengineering, Advanced Medical Engineering Center, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yanagida
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okada
authors.forename: Miwa
authors.initials: M
authors.lastname: Masuda
authors.forename: Isao
authors.initials: I
authors.lastname: Narama
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Nakano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kitao
authors.forename: Kazuo
authors.initials: K
authors.lastname: Takakuda
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Furuzono
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D053758:Nanoparticles / Q000648:ultrastructure; D010006:Osteoblasts / Q000187:drug effects*; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000187:drug effects*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite