title: Tracking viral movement in plants by means of chlorophyll fluorescence imaging.
abstract: Many techniques have been applied to understand viral cell-to-cell movement in host plants, but little progress has been made in understanding viral vascular transport mechanisms. We propose the use of chlorophyll fluorescence imaging techniques, not only to diagnose the viral infection, but also to follow the movement of the virus through the vascular system and its subsequent spread into the leaves. In Nicotiana benthamiana plants, imaging of chlorophyll fluorescence parameters such as Ф(PSII) and NPQ proved useful to follow infections with Pepper mild mottle virus. The results demonstrate a correlation between changes in the chlorophyll fluorescence parameters and the viral distribution analyzed by tissue printing.
authors.affiliation: Department of Biochemistry, Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, C/ Profesor Albareda, n° 1, C.P. 18008, Granada, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Pineda
authors.forename: Julie
authors.initials: J
authors.lastname: Olejníčková
authors.forename: Ladislav
authors.initials: L
authors.lastname: Cséfalvay
authors.forename: Matilde
authors.initials: M
authors.lastname: Barón
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D061848:Optical Imaging; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000648:ultrastructure / Q000821:virology; D014026:Nicotiana / Q000648:ultrastructure / Q000821:virology*; D017898:Tobamovirus / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D002734:Chlorophyll