title: [The roles of PPAR-gamma/PGC-1alpha to Nrf2/gamma-GCS-h in lung of guinea pigs with bronchial asthma].
abstract: OBJECTIVE
  To investigate the expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h in lung of guinea pigs with bronchial asthma, and to explore the roles of them.
  
  
  METHODS
  Forty adult male guinea pigs were randomly divided into 4 groups: the control group (group A), asthmatic group ( group B), dexamethasone group (group C) and rogridone group (group D), 10 guinea pigs in each group. The asthmatic model was established by the ovalbumin challenge method. Expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h mRNA in lung tissue were assayed by in situ hybridization. Expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h protein were detected by immunohischemistry and by Western blot.
  
  
  RESULTS
  In situ hybridization showed that the expressions of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h mRNA in lung tissue were the lowest in group B and the comparison among groups showed statistical significant (all P < 0.01). Immunohistochemistry and Western blot indicated that the value of PPAR-gamma/PGC-1alpha and Nrf2/gamma-GCS-h protein in lung tissue were the lowest in group B, and expressed primarily in nucleus, the differences being statistically significant (all P < 0.01). There was positive correlation between PPAR-gamma and PGC-1. gamma-GCS-h mRNA also positively correlated between PPAR-gamma/PGC-1alpha and Nrf2 in nucleus, and the expression of Nrf2 was also positively correlated with PPAR-gamma/ PGC-1alpha.
  
  
  CONCLUSIONS
  In acute asthmatic models induced by ovalbumin, the expressions of PPAR-alpha/PGC-1alpha and Nrf2/gamma-GCS-h were decreased, and PPARgamma/PGC-1alpha could up-regulate the expressions of Nrf2/gamma-GCS-h to increase the antioxidant defense of tissues, thus being implicated that PPARgamma/PGC-1alpha might play important roles in the pathogenesis and prevention of asthma.
authors.affiliation: The Seventh Ward of Thoracic Center, Changsha Central Hospital, Changsha 410004, China. chenjian8110@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Dai
authors.forename: Man-Jiao
authors.initials: MJ
authors.lastname: Fu
authors.forename: Zhang-Gai
authors.initials: ZG
authors.lastname: Long
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000503:physiopathology*; D005721:Glutamate-Cysteine Ligase / Q000235:genetics / Q000378:metabolism*; D006168:Guinea Pigs; D008168:Lung / Q000378:metabolism*; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D010047:Ovalbumin; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D051267:NF-E2-Related Factor 2; D047495:PPAR gamma; D012333:RNA, Messenger; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; D010047:Ovalbumin; D005721:Glutamate-Cysteine Ligase