title: [Simultaneous determination of 4 major components in semen cassiae obtusifoline by HPLC].
abstract: OBJECTIVE
  To establish a HPLC method for the simultaneous determination of 4 major components in semen cassiae obtusifoline and provide valuable data for quality control of semen cassiae obtusifoline.
  
  
  METHODS
  Kromasil 100-5 C18 column (4.6 mm x 250 mm, 5 microm). Eluted with water: acetonitrile: tetramethylene oxide: glacial acetic acid (100: 23: 5:1). Detecte at 278 nm. The flow rate was 1.0 mL x min(-1).
  
  
  RESULTS
  Four compositions were separated well. Good linearies were obtained within the range of 0.274-1.37 microg, 0.153-0.765 microg, 0.302-1.51 microg and 0.052-0.26 microg for rubrofusarin gentiobioside, casside, aurantio-obtusin-6-O-beta-D- glucopyranoside, casside B respectively. The average recoveries were 100.9% (RSD 1.8%),101.2% (RSD 1.2%), 99.40% (RSD 2.2%), 100.5% (RSD 1.6%).
  
  
  CONCLUSIONS
  The method is accurate, reliable and convenient. It can be used to control the quality of semen cassiae obtusifoline and its products.
authors.affiliation: Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Su
authors.forename: Zhuju
authors.initials: Z
authors.lastname: Wang
authors.forename: Liying
authors.initials: L
authors.lastname: Tang
mesh_terms: D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal