title: Topology of 1-acyl-sn-glycerol-3-phosphate acyltransferases SLC1 and ALE1 and related membrane-bound O-acyltransferases (MBOATs) of Saccharomyces cerevisiae.
abstract: In yeast, phosphatidic acid, the biosynthetic precursor for all glycerophospholipids and triacylglycerols, is made de novo by the 1-acyl-sn-glycerol-3-phosphate acyltransferases Ale1p and Slc1p. Ale1p belongs to the membrane-bound O-acyltransferase (MBOAT) family, which contains many enzymes acylating lipids but also others that acylate secretory proteins residing in the lumen of the ER. A histidine present in a very short loop between two predicted transmembrane domains is the only residue that is conserved throughout the MBOAT gene family. The yeast MBOAT proteins of known function comprise Ale1p, the ergosterol acyltransferases Are1p and Are2p, and Gup1p, the last of which acylates lysophosphatidylinositol moieties of GPI anchors on ER lumenal GPI proteins. C-terminal topology reporters added to truncated versions of Gup1p yield a topology predicting a lumenal location of its uniquely conserved histidine 447 residue. The same approach shows that Ale1p and Are2p also have the uniquely conserved histidine residing in the ER lumen. Because these data raised the possibility that phosphatidic acid could be made in the lumen of the ER, we further investigated the topology of the second yeast 1-acyl-sn-glycerol-3-phosphate acyltransferase, Slc1p. The location of C-terminal topology reporters, microsomal assays probing the protease sensitivity of inserted tags, and the accessibility of natural or artificially inserted cysteines to membrane-impermeant alkylating agents all indicate that the most conserved motif containing the presumed active site histidine of Slc1p is oriented toward the ER lumen, whereas other conserved motifs are cytosolic. The implications of these findings are discussed.
authors.affiliation: Department of Biology, University of Fribourg, CH-1700 Fribourg, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Pagac
authors.forename: Hector Vazquez
authors.initials: HV
authors.lastname: de la Mora
authors.forename: Cécile
authors.initials: C
authors.lastname: Duperrex
authors.forename: Carole
authors.initials: C
authors.lastname: Roubaty
authors.forename: Christine
authors.initials: C
authors.lastname: Vionnet
authors.forename: Andreas
authors.initials: A
authors.lastname: Conzelmann
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D004398:Dyneins; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000235:genetics; D007425:Intracellular Membranes / Q000201:enzymology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D002785:Sterol O-Acyltransferase / Q000235:genetics / Q000378:metabolism
chemical_list: C414103:Gup1 protein, S cerevisiae; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D000217:Acyltransferases; C543388:ARE2 protein, S cerevisiae; D002785:Sterol O-Acyltransferase; D004398:Dyneins; C083745:SLC1 protein, S cerevisiae