title: Quantitative analysis of myo-inositol in urine, blood and nutritional supplements by high-performance liquid chromatography tandem mass spectrometry.
abstract: Myo-inositol plays key physiological functions, necessitating development of methodology for quantification in biological matrices. Limitations of current mass spectrometry-based approaches include the need for a derivatisation step and/or sample clean-up. In addition, co-elution of glucose may cause ion suppression of myo-inositol signals, for example in blood or urine samples. We describe an HPLC-MS/MS method using a lead-form resin based column online to a triple quadrupole tandem mass spectrometer, which requires minimum sample preparation and no derivatisation. This method allows separation and selective detection of myo-inositol from other inositol stereoisomers. Importantly, inositol was also separated from hexose monosaccharides of the same molecular weight, including glucose, galactose, mannose and fructose. The inter- and intra-assay variability was determined for standard solutions and urine with inter-assay coefficient of variation (CV) of 1.1% and 3.5% respectively, while intra-assay CV was 2.3% and 3.6%. Urine and blood samples from normal individuals were analysed.
authors.affiliation: Neural Development Unit, Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, United Kingdom.
authors.forename: Kit-Yi
authors.initials: KY
authors.lastname: Leung
authors.forename: Kevin
authors.initials: K
authors.lastname: Mills
authors.forename: Katie A
authors.initials: KA
authors.lastname: Burren
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Copp
authors.forename: Nicholas D E
authors.initials: ND
authors.lastname: Greene
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019587:Dietary Supplements / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000032:analysis* / Q000097:blood / Q000652:urine; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007294:Inositol; D005947:Glucose