title: Variants of HSPA1A in combination with plasma Hsp70 and anti-Hsp70 antibody levels associated with higher risk of acute coronary syndrome.
abstract: OBJECTIVE
  It was the aim of our study to investigate whether polymorphisms of HSP70 have an affect on antigen and antibody levels in acute coronary syndrome (ACS) patients and normal controls, and the possible joint effect of variants and antigen and antibody levels on the risk of ACS.
  
  
  METHODS
  Three single nucleotide polymorphisms of HSPA1A and HSPA1L were evaluated in 520 ACS patients and 520 age- and sex-matched controls. Plasma extracellular Hsp70 (eHsp70) and anti-Hsp70 antibody levels were determined using ELISA.
  
  
  RESULTS
  Individuals with +190G/C (rs1043618) CC genotype in HSPA1A had higher levels of eHsp70 in controls and lower levels of anti-Hsp70 body in ACS, compared with +190G/C GG carriers. Significantly increased ACS risks of 2.93 and 3.53 fold were found in subjects with the +190G/C CC genotype and high eHsp70 levels or low anti-Hsp70 antibody levels, respectively. The highest risk of ACS was found in subjects with +190G/C CC genotypes, high eHsp70 and low anti-Hsp70 antibody levels compared with those in the reference group (OR = 7.57, 95% CI 3.04-18.87).
  
  
  CONCLUSIONS
  The +190G/C polymorphism of HSPA1A may contribute to influence eHsp70 levels in controls and anti-Hsp70 antibody levels in ACS, and the +190G/C genotypes, eHsp70 and anti-Hsp70 antibody levels may have a joint effect on the risk of ACS.
authors.affiliation: Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. mingxz117@mail.hust.edu.cn
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Zhang
authors.forename: Robert M
authors.initials: RM
authors.lastname: Tanguay
authors.forename: Mei'an
authors.initials: M
authors.lastname: He
authors.forename: Qifei
authors.initials: Q
authors.lastname: Deng
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Miao
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Tangchun
authors.initials: T
authors.lastname: Wu
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000235:genetics* / Q000276:immunology; D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000097:blood; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D000906:Antibodies; D000941:Antigens; D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human; C543454:HSPA1L protein, human