title: Platelet Activation Following Exposure to Anti-ABO Antibodies-An In Vitro Study.
abstract: Since platelets possess A and B antigen, mismatched ABO platelets could, theoretically, become activated or hypofunctional by exposure to anti-A or anti-B antibodies found in transfused or recipient plasma. Following normal baseline platelet aggregation to adenosine diphosphate (ADP), platelets from normal donors of different blood types were incubated at 37°C for 10 minutes with 50μl of normal saline (NS), O plasma, or AB plasma. Aggregation was then induced with ADP. No significant changes from baseline were seen in platelet aggregation studies following incubation with NS. However, platelet aggregations of type A and type B platelets were significantly inhibited when incubated with O plasma (mean of 41 and 22%, respectively). Our findings indicate that mediators in group O plasma, very likely anti-A and anti-B antibodies, cause impaired platelet aggregation of ABO non-identical platelets.
authors.affiliation: Assistant Professor, Department of Medicine, University of Rochester Medical Center.
authors.forename: Majed A
authors.initials: MA
authors.lastname: Refaai
authors.forename: Kelly F
authors.initials: KF
authors.lastname: Henrichs
authors.forename: Sherry L
authors.initials: SL
authors.lastname: Spinelli
authors.forename: Richard P
authors.initials: RP
authors.lastname: Phipps
authors.forename: Edward
authors.initials: E
authors.lastname: Masel
authors.forename: Brian H
authors.initials: BH
authors.lastname: Smith
authors.forename: Charles W
authors.initials: CW
authors.lastname: Francis
authors.forename: Neil
authors.initials: N
authors.lastname: Blumberg