title: Cytoplasmic N-glycosyltransferase of Actinobacillus pleuropneumoniae is an inverting enzyme and recognizes the NX(S/T) consensus sequence.
abstract: N-Linked glycosylation is a frequent protein modification that occurs in all three domains of life. This process involves the transfer of a preassembled oligosaccharide from a lipid donor to asparagine side chains of polypeptides and is catalyzed by the membrane-bound oligosaccharyltransferase (OST). We characterized an alternative bacterial pathway wherein a cytoplasmic N-glycosyltransferase uses nucleotide-activated monosaccharides as donors to modify asparagine residues of peptides and proteins. N-Glycosyltransferase is an inverting glycosyltransferase and recognizes the NX(S/T) consensus sequence. It therefore exhibits similar acceptor site specificity as eukaryotic OST, despite the unrelated predicted structural architecture and the apparently different catalytic mechanism. The identification of an enzyme that integrates some of the features of OST in a cytoplasmic pathway defines a novel class of N-linked protein glycosylation found in pathogenic bacteria.
authors.affiliation: Institutes of Microbiology, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland.
authors.forename: Flavio
authors.initials: F
authors.lastname: Schwarz
authors.forename: Yao-Yun
authors.initials: YY
authors.lastname: Fan
authors.forename: Mario
authors.initials: M
authors.lastname: Schubert
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D003593:Cytoplasm / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000737:chemistry*; D006031:Glycosylation; D006602:Hexosyltransferases / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry; D009005:Monosaccharides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D009005:Monosaccharides; D005964:Glucosyltransferases; D006602:Hexosyltransferases; C410788:N-glycosyltransferase, Nocardia aerocolonigenes; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D005947:Glucose