title: An approach to architecture 3D scaffold with interconnective microchannel networks inducing angiogenesis for tissue engineering.
abstract: The angiogenesis of 3D scaffold is one of the major current limitations in clinical practice tissue engineering. The new strategy of construction 3D scaffold with microchannel circulation network may improve angiogenesis. In this study, 3D poly(D: ,L: -lactic acid) scaffolds with controllable microchannel structures were fabricated using sacrificial sugar structures. Melt drawing sugar-fiber network produced by a modified filament spiral winding method was used to form the microchannel with adjustable diameters and porosity. This fabrication process was rapid, inexpensive, and highly scalable. The porosity, microchannel diameter, interconnectivity and surface topographies of the scaffold were characterized by scanning electron microscopy. Mechanical properties were evaluated by compression tests. The mean porosity values of the scaffolds were in the 65-78% and the scaffold exhibited microchannel structure with diameter in the 100-200 μm range. The results showed that the scaffolds exhibited an adequate porosity, interconnective microchannel network, and mechanical properties. The cell culture studies with endothelial cells (ECs) demonstrated that the scaffold allowed cells to proliferate and penetrate into the volume of the entire scaffold. Overall, these findings suggest that the fabrication process offers significant advantages and flexibility in generating a variety of non-cytotoxic tissue engineering scaffolds with controllable distributions of porosity and physical properties that could provide the necessary physical cues for ECs and further improve angiogenesis for tissue engineering.
authors.affiliation: Key Lab of Biorheological Science and Technology, College of Bioengineering, Ministry of Education, Research Center of Bioinspired Material Science and Engineering, Chongqing University, Chongqing 400030, China.
authors.forename: Jiaoxia
authors.initials: J
authors.lastname: Sun
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Qian
authors.forename: Chenbo
authors.initials: C
authors.lastname: Hu
mesh_terms: D055359:Antistatic Agents; D001672:Biocompatible Materials; D002478:Cells, Cultured; D042783:Endothelial Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000502:physiology*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D055359:Antistatic Agents; D001672:Biocompatible Materials