title: Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma.
abstract: Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.
authors.affiliation: School of Chemical and Biological Engineering Seoul National University Seoul 151-744, Korea.
authors.forename: Hee Seok
authors.initials: HS
authors.lastname: Yang
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Shin
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.forename: Jung Youn
authors.initials: JY
authors.lastname: Shin
authors.forename: Jooyeon
authors.initials: J
authors.lastname: Park
authors.forename: Gun Il
authors.initials: GI
authors.lastname: Im
authors.forename: Chang Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000077214:Becaplermin; D015153:Blotting, Western; D049109:Cell Proliferation; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D005260:Female; D005337:Fibrin / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D007124:Immunoenzyme Techniques; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration; D012867:Skin / Q000166:cytology* / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin; D005337:Fibrin; D006493:Heparin