title: In vitro expansion of human adipose-derived stem cells in a spinner culture system using human extracellular matrix powders.
abstract: Stem cell therapy requires large numbers of stem cells to replace damaged tissues, but only limited numbers of stem cells can be harvested from a single patient. To obtain large quantities of stem cells with differentiation potential, we explored a spinner culture system using human extracellular matrix (hECM) powders. The hECM was extracted from adipose tissue and fabricated into powders. Human adipose-derived stem cells (hASCs) were isolated, seeded on hECM powders, and cultivated in a spinner flask. The 3-D culture system, using hECM powders, was highly effective for promoting cell proliferation. The number of hASCs in the 3-D culture system significantly increased for 10 days, resulting in an approximately 10-fold expansion, whereas a traditional 2-D culture system showed just a 2.8-fold expansion. Surface markers, transcriptional factors, and differentiation potential of hASCs were assayed to identify the characteristics of proliferated cells in 3-D culture system. The hASCs expressed the pluripotency markers, Oct-4 and Sox-2 during 3-D culture and retained their capacity to differentiate into adipogenic, osteogenic, and chondrogenic lineages. These findings demonstrate that the 3-D culture systems using hECM powders provide an efficient in vitro environment for stem cell proliferation, and could act as stem cell delivery carriers for autologous tissue engineering and cell therapy.
authors.affiliation: Departments of Chemical Engineering and Bionanotechnology, Hanyang University, Sangnok-gu, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Ji Suk
authors.initials: JS
authors.lastname: Choi
authors.forename: Beob Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Jae Dong
authors.initials: JD
authors.lastname: Kim
authors.forename: Young Chan
authors.initials: YC
authors.lastname: Choi
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Lee
authors.forename: Kinam
authors.initials: K
authors.lastname: Park
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Yong Woo
authors.initials: YW
authors.lastname: Cho
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D039904:Pluripotent Stem Cells / Q000166:cytology; D011208:Powders; D013234:Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D011208:Powders