title: Pressure-assisted electrokinetic supercharging for the enhancement of non-steroidal anti-inflammatory drugs.
abstract: Electrokinetic supercharging (EKS) combines field-amplified sample injection with transient isotachophoresis (tITP) to create a powerful on-line preconcentration technique for capillary electrophoresis. In this work, EKS is enhanced with a positive pressure (pressure-assisted EKS, or PA-EKS) during injection to improve stacking of non-steroidal anti-inflammatory drugs (NSAIDs). Several parameters, including buffer composition and concentration, terminating electrolyte, organic modifier, and injection voltage and injection time of both terminating electrolyte and sample were optimized. Detection limits for seven NSAIDs were determined and an enhancement in sensitivity of almost 50,000-fold was obtained. The PA-EKS method has the potential to be a simple MS compatible preconcentration method to improve the sensitivity of CE.
authors.affiliation: Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
authors.forename: Michelle M
authors.initials: MM
authors.lastname: Meighan
authors.forename: Mohamed
authors.initials: M
authors.lastname: Dawod
authors.forename: Rosanne M
authors.initials: RM
authors.lastname: Guijt
authors.forename: Mark A
authors.initials: MA
authors.lastname: Hayes
authors.forename: Michael C
authors.initials: MC
authors.lastname: Breadmore
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D057625:Isotachophoresis / Q000295:instrumentation / Q000379:methods*; D011312:Pressure; D012680:Sensitivity and Specificity
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal