title: [Applications and perspectives of DNA stable-isotope probing in metagenomics: a review].
abstract: DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D056186:Metagenomics / Q000379:methods*; D015336:Molecular Probe Techniques; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004247:DNA