title: A novel method of core aspirate cytology compared to fine-needle aspiration for diagnosing canine osteosarcoma.
abstract: There is little information in veterinary literature regarding the diagnostic accuracy of aspirate cytology for the diagnosis of canine osteosarcoma (OSA). The authors compared the diagnostic accuracy of a novel method of cytologic collection, termed core aspirate cytology (CA), with fine needle aspiration (FNA) and histopathology in 27 dogs with lytic and/or proliferative bone lesions. Alkaline phosphatase (ALP) staining was performed to confirm the diagnosis of OSA cytologically. OSA was accurately diagnosed in 85% and 95% of FNA and CA, respectively. ALP staining was 100% sensitive for the diagnosis of OSA. CA using a bone marrow biopsy needle allowed for penetration of cortical bone and aspirate cytology with a larger bore needle than FNA; however, there was no significant difference in diagnostic accuracy between techniques. Aspirate cytology with ALP staining was a safe, accurate, and minimally invasive diagnostic test for the evaluation of suspected OSA lesions in dogs.
authors.affiliation: Affiliated Veterinary Specialists, Orange Park, FL, USA. sneihausdvm@hotmail.com
authors.forename: Steven A
authors.initials: SA
authors.lastname: Neihaus
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Locke
authors.forename: Anne M
authors.initials: AM
authors.lastname: Barger
authors.forename: Luke B
authors.initials: LB
authors.lastname: Borst
authors.forename: Robert L
authors.initials: RL
authors.lastname: Goring
mesh_terms: D000818:Animals; D044963:Biopsy, Fine-Needle / Q000662:veterinary; D001707:Biopsy, Needle / Q000662:veterinary*; D001859:Bone Neoplasms / Q000175:diagnosis* / Q000473:pathology; D004283:Dog Diseases / Q000175:diagnosis* / Q000473:pathology; D004285:Dogs; D005260:Female; D008297:Male; D012516:Osteosarcoma / Q000175:diagnosis / Q000662:veterinary*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000662:veterinary