title: Phenotypic and molecular characterization of Yersinia ruckeri isolates from rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) in Turkey.
abstract: The aim of the study was the phenotypic and molecular characterization of Yersinia (Y) ruckeri strains, the causative agent of Enteric Redmouth Disease (ERM), by antibiotyping, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of whole cell proteins. For this aim, a total of 97 Y ruckeri isolates were analyzed. The isolates were distinguished into ten antibiotypes and six phenotypes according to their resistance properties and whole cell protein profiles, respectively. Also, a glycoprotein band of approximately 25.5 kDa was observed in all Y ruckeri strains tested. In all strains, six different RAPD types were observed. In conclusion, Y ruckeri strains isolated from rainbow trout of fish farms in Turkey showed variation according to their phenotypic and genotypic characteristics, and the use of these three typing techniques in double and triple combinations could be more useful for discriminating the strains.
authors.affiliation: Department of Diseases and Clinical Sciences, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun,Turkey. eeonuk@omu.edu.tr
authors.forename: Ertan Emek
authors.initials: EE
authors.lastname: Onuk
authors.forename: Alper
authors.initials: A
authors.lastname: Ciftci
authors.forename: Arzu
authors.initials: A
authors.lastname: Findik
authors.forename: Gülay
authors.initials: G
authors.lastname: Ciftci
authors.forename: Soner
authors.initials: S
authors.lastname: Altun
authors.forename: Fikri
authors.initials: F
authors.lastname: Balta
authors.forename: Selmin
authors.initials: S
authors.lastname: Ozer
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D005393:Fish Diseases / Q000382:microbiology*; D005838:Genotype; D006023:Glycoproteins / Q000032:analysis; D008826:Microbial Sensitivity Tests; D017686:Oncorhynchus mykiss; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015203:Reproducibility of Results; D013045:Species Specificity; D014421:Turkey; D015009:Yersinia Infections / Q000382:microbiology / Q000662:veterinary*; D044124:Yersinia ruckeri / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006023:Glycoproteins