title: Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures.
abstract: There is evidence that pro-opiomelanocortin (POMC)-derived peptides other than adrenocorticotropic hormone (ACTH) have a role in adrenal cell proliferation. We compared the activity of synthetic rat N-terminal POMC fragment 1-28 with disulfide bridges (N-POMC(w)) and without disulfide bridges (N-POMC(w/o)), with the activity of fibroblast growth factor (FGF2), a widely studied adrenal growth factor, and ACTH, in well-characterized pure cultures of both isolated adrenal Glomerulosa (G) and Fasciculata/Reticularis (F/R) cells. Three days of FGF2-treatment had a proliferative effect similar to serum, and synthetic peptide N-POMC(w) induced proliferation more efficiently than N-POMC(w/o). Moreover, both induced proliferation via the ERK1/2 pathway. In contrast, sustained ACTH treatment decreased proliferation and viability through apoptosis induction, but not necrosis, and independently of PKA and PKC pathways. Further elucidation of 1-28 POMC signal transduction is of interest, and primary cultures of adrenal cells were found to be useful for examining the trophic activity of this peptide.
authors.affiliation: Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
authors.forename: Gabriele E
authors.initials: GE
authors.lastname: Mattos
authors.forename: Jacqueline F
authors.initials: JF
authors.lastname: Jacysyn
authors.forename: Gustavo P
authors.initials: GP
authors.lastname: Amarante-Mendes
authors.forename: Claudimara Ferini Pacicco
authors.initials: CF
authors.lastname: Lotfi
mesh_terms: D000311:Adrenal Glands / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001391:Azo Compounds / Q000378:metabolism; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011333:Pro-Opiomelanocortin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D001391:Azo Compounds; C508137:Creb1 protein, rat; D017362:Cyclic AMP Response Element-Binding Protein; D010455:Peptides; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D013252:Steroid 11-beta-Hydroxylase; D014446:Tyrosine 3-Monooxygenase; D048049:Extracellular Signal-Regulated MAP Kinases; C011049:oil red O; D006416:Hematoxylin