title: Efficient biotinylation of methacryloyl-functionalized nonadherent cells for formation of cell microarrays.
abstract: In order to obtain cell microarrays formed with human promyelocytic leukemia cells (HL-60), biotinylation of methacryloyl-functionalized HL-60 cells was performed via a thiol-ene reaction with thiol-terminated 4-arm poly(ethylene glycol) (PEG(4)10K-SH). Biotinylated HL-60 cells were selectively adhered onto an avidin-patterned surface with high viability.
authors.affiliation: Department of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita-shi, Osaka 564-8680, Japan. yasu.bmt@kansai-u.ac.jp
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ota
mesh_terms: D019921:Biotinylation; D049109:Cell Proliferation; D018922:HL-60 Cells; D006595:Hexosamines / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D046888:Tissue Array Analysis / Q000379:methods*
chemical_list: D006595:Hexosamines; D013438:Sulfhydryl Compounds; C008322:mannosamine; D011092:Polyethylene Glycols