title: Molecular water motions of skim milk powder solutions during acidification studied by 17O and 1H nuclear magnetic resonance and rheology.
abstract: The molecular motion of water was studied in glucono-δ-lactone-acidified skim milk powder (SMP) solutions with various pH values and dry matter contents. NMR relaxometry measurements revealed that lowering the pH in SMP solutions affected 17O and 1H T2 relaxation rates almost identically. Consequently, the present study indicates that the proteins present in the samples do not affect the 1H relaxation behavior markedly, even at relatively high SMP concentrations (15-25%). Comparison of rheological measurements and NMR measurements suggested that the collapse of κ-casein during acidification could contribute to the initial decrease in 17O and 1H relaxation rate in the pH range between 6.6 and 5.5 for 15% SMP and in the pH range between 6.6 and 5.9 for 25% SMP. However, below pH 5.5 the viscosity and 17O and 1H NMR relaxation rates did not correlate, revealing that the aggregation of casein micelles, which increases viscosity below pH 5.5, does not involve major repartitioning of water.
authors.affiliation: Department of Food Science, Faculty of Science and Technology, Aarhus University, Research Centre Aarslev, Kirstinebjergvej 10, DK-5792 Årslev, Denmark.
authors.forename: Sandie M
authors.initials: SM
authors.lastname: Møller
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Whittaker
authors.forename: Jason R
authors.initials: JR
authors.lastname: Stokes
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Gidley
authors.forename: Ulf
authors.initials: U
authors.lastname: Andersen
authors.forename: Hanne C
authors.initials: HC
authors.lastname: Bertram
mesh_terms: D000818:Animals; D002364:Caseins / Q000737:chemistry; D057141:Food, Preserved; D005942:Gluconates; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007783:Lactones; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000737:chemistry*; D010103:Oxygen Isotopes; D012212:Rheology; D012996:Solutions; D014783:Viscosity; D014867:Water / Q000737:chemistry*
chemical_list: D002364:Caseins; D005942:Gluconates; D007783:Lactones; D010103:Oxygen Isotopes; D012996:Solutions; D014867:Water; D006859:Hydrogen; C010730:beta-glucono-1,5-lactone