title: [Study for association between high risk human papillomavirus and cervical lesions in the samples from opportunistic screening].
abstract: OBJECTIVE
  To investigate the association between HPV genotypes and cervical lesion in Hybrid Capture 2 (HC2) HPV test positive samples.
  
  
  METHODS
  602 cervical samples randomly obtained detected as HPV positive by the HC2 high-risk probe cocktail were determined by polymerase chain reaction (PCR)-reverse dot blot (RDB), among them 344 participated Thinprep Cytology test.
  
  
  RESULTS
  569 (94.5%) samples were successfully amplified. The most common HPV genotypes were HPV16 (31.6%), 52 (16.7%), 58 (15.1%), 56 (8.1%), 39 (7.9%); HPV16 or HPV16/18 infection was significantly related to ASCUS, LSIL and HSIL; 24 samples suggested single HPV infection with the genotypes not available for HC2.
  
  
  CONCLUSIONS
  The most common HPV genotypes in random screening were HPV16, 52 and HPV58; Our data demonstrated the pseudo-positivity of HC2 test was 4.0%; HPV16 was a index for progression of HSIL.
authors.affiliation: Department of Obstetrics and Gynaecology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Bian
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Wang
authors.forename: Xiao-zhou
authors.initials: XZ
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.forename: Hong-tu
authors.initials: HT
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002584:Cervix Uteri / Q000473:pathology / Q000821:virology; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D012307:Risk Factors; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult