title: The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits.
abstract: The nuclear export of large ribonucleoparticles is complex and requires specific transport factors. Messenger RNAs are exported through the RNA-binding protein Npl3 and the interacting export receptor Mex67. Export of large ribosomal subunits also requires Mex67; however, in this case, Mex67 binds directly to the 5S ribosomal RNA (rRNA) and does not require the Npl3 adaptor. Here, we have discovered a new function of Npl3 in mediating the export of pre-60S ribosomal subunit independently of Mex67. Npl3 interacts with the 25S rRNA, ribosomal and ribosome-associated proteins, as well as with the nuclear pore complex. Mutations in NPL3 lead to export defects of the large subunit and genetic interactions with other pre-60S export factors.
authors.affiliation: Abteilung für Molekulare Genetik, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August Universität Göttingen, Grisebachstrasse 8, Göttingen 37077, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Hackmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Gross
authors.forename: Claudia
authors.initials: C
authors.lastname: Baierlein
authors.forename: Heike
authors.initials: H
authors.lastname: Krebber
mesh_terms: D021581:Active Transport, Cell Nucleus; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D009154:Mutation / Q000235:genetics; D022022:Nuclear Pore / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D054658:Ribosome Subunits, Large / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C077734:NPL3 protein, S cerevisiae; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins