title: [Clinical evaluation of insulin measuring kit not cross-react with insulin analogue preparations].
abstract: Clinical evaluation of insulin assay system reacting with only human insulin molecule (kit B) was performed by comparing it with conventional insulin assay system (kit A) cross-reacting with insulin analogue as well as human insulin preparation. In vitro, the kit B was confirmed to cross-react with only human insulin, not with insulin analogue preparations such as insulin aspart, lyspro and glargine. In non-insulin treated diabetic patients, postprandial and post-insulin injected serum immunoreactive insulin (IRI) concentrations measured by kit B were almost the same as those measured by the kit A. On the other hand, in diabetic patients treated with insulin analogue preparations, postprandial and post-insulin injected serum IRI levels measured by kit B were obviously low compared with those by kit A. After intravenous injection of insulin analogue preparations (0.1 unit/kg), insulin lyspro or insulin aspart, serum IRI levels measured by the kit B were not increased but gradually decreased in contrast to the obviously increased serum IRI level measured by the kit A. From these results, the kit B was confirmed not to measure the insulin analogue preparations in vitro and in vivo.
authors.affiliation: Department of Clinical Laboratory Medicine, Wakayama Medical University, Wakayama 641-8509, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Ueyama
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shimajiri
authors.forename: Shuhei
authors.initials: S
authors.lastname: Morita
authors.forename: Akiko
authors.initials: A
authors.lastname: Yamana
authors.forename: Machi
authors.initials: M
authors.lastname: Furuta
authors.forename: Tokio
authors.initials: T
authors.lastname: Sanke
mesh_terms: D003429:Cross Reactions; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy; D006801:Humans; D007275:Injections, Intravenous; D007328:Insulin / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000097:blood*; D061267:Insulin Aspart; D000069036:Insulin Glargine; D061268:Insulin Lispro; D049528:Insulin, Long-Acting; D011933:Reagent Kits, Diagnostic
chemical_list: D007328:Insulin; D061268:Insulin Lispro; D049528:Insulin, Long-Acting; D011933:Reagent Kits, Diagnostic; D000069036:Insulin Glargine; D061267:Insulin Aspart