title: Olig2 lineage cells generate GABAergic neurons in the prethalamic nuclei, including the zona incerta, ventral lateral geniculate nucleus and reticular thalamic nucleus.
abstract: Neuronal differentiation is a crucial event during neural development. Recent studies have characterized the development of the diencephalon; however, the origins of the primarily GABAergic prethalamic nuclei, including the zona incerta (ZI), ventral lateral geniculate nucleus (vLG) and reticular thalamic nucleus (RT), remain unclear. Here we characterize Olig2 lineage cells in the developing prethalamus using mice in which tamoxifen-induced recombination permanently labels Olig2-expressing cells. We show that GABAergic neurons in the prethalamic nuclei, including the RT, ZI and vLG, originate from prethalamic Olig2 lineage cells. Based on these data and on those derived from short-term lineage-tracing data using Olig3-lacZ mice and previous reports, we suggest that vLG cells originate from the ventricular zone of the thalamus, zona limitans intrathalamica and prethalamus.
authors.affiliation: Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Inamura
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ono
authors.forename: Hirohide
authors.initials: H
authors.lastname: Takebayashi
authors.forename: Bernard
authors.initials: B
authors.lastname: Zalc
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikenaka
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D059330:GABAergic Neurons / Q000166:cytology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005829:Geniculate Bodies / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D013234:Stem Cells / Q000502:physiology; D020530:Subthalamus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013629:Tamoxifen; D020651:Ventral Thalamic Nuclei / Q000166:cytology / Q000196:embryology / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D013629:Tamoxifen; C512773:Olig2 protein, mouse; C420992:Olig3 protein, mouse