title: Effect of neuraminidase treatment on persistent epileptiform activity in the rat hippocampus.
abstract: Negatively charged sialic acid residues located close to pores of voltage-gated sodium channels substantially influence their gating properties. The in vitro low Mg²⁺ seizure model is used to emulate difficult-to-treat status epilepticus. Using this model on cultured hippocampal slices, we examined the effectiveness of desialylation in reducing persistent seizure-like activity. We show that desialylation in cultured hippocampal slices effectively suppresses seizure-like activity induced by low Mg²⁺. These findings suggest that targeting negatively charged sialic acids may be an effective strategy to treat status epilepticus.
authors.affiliation: Department of General Physiology of Nervous System, Bogomoletz Institute of Physiology, Bogomoletz st. 4, Kiev, Ukraine. dmytro.isaev@gmail.com
authors.forename: Elena
authors.initials: E
authors.lastname: Isaeva
authors.forename: Irina
authors.initials: I
authors.lastname: Lushnikova
authors.forename: Alina
authors.initials: A
authors.lastname: Savrasova
authors.forename: Galina
authors.initials: G
authors.lastname: Skibo
authors.forename: Gregory L
authors.initials: GL
authors.lastname: Holmes
authors.forename: Dmytro
authors.initials: D
authors.lastname: Isaev
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D006624:Hippocampus / Q000473:pathology*; D008274:Magnesium / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012794:Sialic Acids / Q000378:metabolism*; D013226:Status Epilepticus / Q000188:drug therapy* / Q000503:physiopathology
chemical_list: D012794:Sialic Acids; D009439:Neuraminidase; D008274:Magnesium