title: Fabrication of a highly sensitive electrochemiluminescence lactate biosensor using ZnO nanoparticles decorated multiwalled carbon nanotubes.
abstract: ZnO nanoparticles (nanoZnO) were decorated on multiwalled carbon nanotubes (MWCNTs) and then the prepared nano-hybrids, nanoZnO-MWCNTs, were immobilized on the surface of a glassy carbon electrode (GCE) to fabricate nanoZnO-MWCNTs modified GCE. The prepared electrode, GCE/nanoZnO-MWCNTs, showed excellent electrocatalytic activity towards luminol electrochemiluminescence (ECL) reaction. The electrode was then further modified with lactate oxidase and Nafion to fabricate a highly sensitive ECL lactate biosensor. Two linear dynamic ranges of 0.01-10 μmol L(-1) and 10-200 μmol L(-1) were obtained for lactate with the correlation coefficient better than 0.9996. The detection limit (S/N=3) was 4 nmol L(-1) lactate. The relative standard deviation for repetitive measurements (n=6) of 10 μmol L(-1) lactate was 1.5%. The fabrication reproducibility for five biosensors prepared and used in different days was 7.4%. The proposed ECL lactate biosensor was used for determination of lactate in human blood plasma samples with satisfactory results.
authors.affiliation: Department of Chemistry, Institute for Advanced Studies in Basic Sciences, P.O. Box 45195-1159, Gava Zang, Zanjan, Iran. haghighi@iasbs.ac.ir
authors.forename: Behzad
authors.initials: B
authors.lastname: Haghighi
authors.forename: Somayyeh
authors.initials: S
authors.lastname: Bozorgzadeh
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005898:Glass / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D013499:Surface Properties; D013696:Temperature; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D019344:Lactic Acid; D008165:Luminol; D006861:Hydrogen Peroxide; D015034:Zinc Oxide