title: Laminin-5 immunohistochemical mapping for analysis of local extension in hypopharyngeal cancer: A preliminary report.
abstract: OBJECTIVE
  Laminin-5 γ2 chain (LNγ2) plays an important role in cancer differentiation and migration. Using a novel immunohistochemical mapping technique to investigate whole mucosal strips of total laryngopharyngectomy specimens using iodine, H-E, and LNγ2 stainings, we investigated the locoregional behavior of hypopharyngeal cancer.
  
  
  METHODS
  Surgical specimens from two patients with pyriform sinus cancer were investigated. Three percent iodine was applied to the tumor-bearing laryngopharynx during surgery and photographed. Stainabilities of H-E and LNγ2 on pathologic sections from all mucosal strips were scored and coordinated with the laryngopharyngeal photograph to illustrate the immunohistochemical map.
  
  
  RESULTS
  In both patients, the main tumor of invasive squamous cell carcinoma was circumferentially surrounded by a superficial lesion characterized by high grade intraepithelial neoplasia that remained unstained by iodine. On LNγ2 immunohistochemical mapping, the main tumor was demonstrated by Score 2 staining and the superficial lesion by a stronger Score 3 staining.
  
  
  CONCLUSIONS
  The finding of neoplastic cells at the periphery demonstrating a higher potential than the cancer cells at the tumor center is suggestive of impending progression from neoplasia to carcinoma. The current preliminary report suggested morphological evidence of intraepithelial infiltration and lateral invasion in hypopharyngeal cancer.
authors.affiliation: Department of Otorhinolaryngology, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 252-0374, Japan. meijin@med.kitasato-u.ac.jp
authors.forename: Meijin
authors.initials: M
authors.lastname: Nakayama
authors.forename: Yu-ichi
authors.initials: Y
authors.lastname: Sato
authors.forename: Keiichi
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: Mikami
authors.initials: M
authors.lastname: Tetuo
authors.forename: Makito
authors.initials: M
authors.lastname: Okamoto
authors.forename: Yutomo
authors.initials: Y
authors.lastname: Seino
authors.forename: Tabito
authors.initials: T
authors.lastname: Okamoto
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Miyamoto
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000175:diagnosis* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000175:diagnosis* / Q000473:pathology; D056144:Pyriform Sinus / Q000473:pathology; D000077195:Squamous Cell Carcinoma of Head and Neck; D000097794:Kalinin
chemical_list: D015815:Cell Adhesion Molecules; D000097794:Kalinin