title: Effects of vaspin, chemerin and omentin-1 on feeding behavior and hypothalamic peptide gene expression in the rat.
abstract: Visceral adipose tissue-derived serpin (vaspin) improves glucose tolerance and insulin sensitivity in diet-induced obese mice. Chemerin may increase insulin sensitivity in adipose tissue and seems to be associated with several key aspects of metabolic syndrome. Decreased levels of omentin-1 are associated with increasing obesity and insulin resistance. Our study aimed to investigate the effects of vaspin, chemerin and omentin-1 acute administration on feeding and hypothalamic gene expression of peptides which play a key role in feeding regulation. 35 rats were injected into the arcuate nucleus (ARC) of the hypothalamus with either saline (n=8), vaspin (1μg/kg; n=9), chemerin (8μg/kg; n=9), or omentin-1 (8μg/kg; n=9). Food intake in the following 24h was recorded, thereafter rats were sacrificed. Total RNA was extracted from hypothalami and reverse transcribed to evaluate hypothalamic gene expression of agouti-related peptide (AgRP), neuropeptide Y (NPY), orexin-A, cocaine- and amphetamine-regulated transcript (CART), corticotrophin releasing hormone (CRH) and proopiomelanocortin (POMC), by real-time reverse transcription polymerase chain reaction. Compared to vehicle, vaspin injection significantly decreased feeding, while chemerin and omentin-1 had no effect in the tested dose. Vaspin treatment significantly decreased NPY and increased POMC gene expression. Chemerin treatment led to a significant increase of both AgRP and POMC gene expression. Omentin-1 treatment did not modify gene expression of the investigated peptides. Therefore, vaspin is an adipokine triggering anorectic pathways in the hypothalamus, where reduction of NPY and increase of POMC mRNA levels mediate feeding inhibition. Chemerin and omentin-1 have no effect on feeding in the tested dose.
authors.affiliation: Department of Drug Sciences, G. d' Annunzio University, via dei Vestini 31, 66013 Chieti, Italy. brunetti@unich.it
authors.forename: Luigi
authors.initials: L
authors.lastname: Brunetti
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Nisio
authors.forename: Lucia
authors.initials: L
authors.lastname: Recinella
authors.forename: Annalisa
authors.initials: A
authors.lastname: Chiavaroli
authors.forename: Sheila
authors.initials: S
authors.lastname: Leone
authors.forename: Claudio
authors.initials: C
authors.lastname: Ferrante
authors.forename: Giustino
authors.initials: G
authors.lastname: Orlando
authors.forename: Michele
authors.initials: M
authors.lastname: Vacca
mesh_terms: D054369:Agouti-Related Protein / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001067:Appetite Depressants / Q000494:pharmacology; D018925:Chemokines / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000008:administration & dosage / Q000494:pharmacology*; D004435:Eating / Q000187:drug effects; D005247:Feeding Behavior / Q000187:drug effects*; D058851:GPI-Linked Proteins / Q000008:administration & dosage / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins; D037102:Lectins / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009478:Neuropeptide Y / Q000235:genetics / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D015843:Serpins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: C515197:AGRP protein, rat; D054369:Agouti-Related Protein; D001067:Appetite Depressants; D018925:Chemokines; D016207:Cytokines; D058851:GPI-Linked Proteins; C432397:ITLN1 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D037102:Lectins; D009478:Neuropeptide Y; D009479:Neuropeptides; C478630:RARRES2 protein, human; D011994:Recombinant Proteins; D015843:Serpins; D011333:Pro-Opiomelanocortin