title: [Mobilization and collection of hematopoietic stem cells in children with different neoplastic diseases for autotransplantation to support high dose chemotherapy (single centre experience)].
abstract: Currently, granulocyte colony stimulating factor (G-CSF) alone or in combination with myelosuppresive chemotherapy remain the standards of CD34+ cells mobilization allows the safe and successful collection of adequate peripheral blood stem cells (PBSC) for autologous transplantation. However, in up to 30% of patients mobilization of PBSC is ineffective. This report presents our experience in mobilization and collection of peripheral blood stem cells in 82 children with different proliferative disease. In mobilization G-CSF was administered alone in steady state (56 patients, pts) or in combination with myelosuppresive chemotherapy (26 pts). The CD34+ cell count at least 10 cells/ml was required to start apheresis procedure, which was repeated, if needed, during following 1-4 days until collection of at least 2 (optimally 3) x106 CD34+ cells/kg b.w. of recipient was obtained. Three pts in each group (3/ 56 and 3/26) failed the first course of mobilization. The median number of CD34+ cells mobilized was 4.8 (0.5-15) x106/kg b.w. The minimal and optimal number of CD34+ cells for transplantation was achieved in 85% and 61% of patients in the G-CSF + chemotherapy group and in 84% and 54% in the G-CSF group, respectively. The efficacy of presented mobilization arms in our group was similar. However, the incidence of infection and total hospitalization time during mobilization were higher in chemotherapy + G-CSF group.
authors.affiliation: Ośrodek Transplantacji Uniwersyteckiego Szpitala Dzieciecego w Krakowie. jgozdzik@cm-uj.krakow.pl
authors.forename: Jolanta
authors.initials: J
authors.lastname: Goździk
authors.forename: Wojciech
authors.initials: W
authors.lastname: Czogała
authors.forename: Szymon
authors.initials: S
authors.lastname: Skoczeń
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Krasowska-Kwiecień
authors.forename: Oktawiusz
authors.initials: O
authors.lastname: Wiecha
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Stec
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czogała
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001781:Blood Component Removal; D002648:Child; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D007223:Infant; D008297:Male; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34