title: Collective sampling of intact anionic polysaccharide components and application in quantitative determination by LC-MS.
abstract: Anionic polysaccharides, such as glycosaminoglycans (GAGs) and alginate, readily undergo source-induced fragmentation when analyzed by electrospray mass spectrometry with the use of high source cone voltage. The dissociation chemistry converts all components of a polysaccharide into a small set of structurally characteristic small saccharides. This chemistry enables the collective detection of a polysaccharide through the detection of one or more small saccharides. This ability, combined with the elution of polysaccharides as relatively compact bands using ion-pairing reverse phase liquid chromatography, created a unique opportunity for the development of LC-MS methods suitable for the quantitative analysis of intact anionic polysaccharides. Feasibility of this approach is demonstrated with a mixture of heparin, chondroitin sulfate A, and alginate.
authors.affiliation: Technology Resources, Baxter Healthcare Corporation, 25212 W. Illinois Route 120, Round Lake, IL 60073, USA. peifeng_hu@baxter.com
authors.forename: Peifeng
authors.initials: P
authors.lastname: Hu
authors.forename: Liqiong
authors.initials: L
authors.lastname: Fang
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Jones
authors.forename: Edward K
authors.initials: EK
authors.lastname: Chess
mesh_terms: D000464:Alginates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006603:Hexuronic Acids / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; C007896:guluronic acid; C008324:mannuronic acid