title: Role of vesicle pools in action potential pattern-dependent dopamine overflow in rat striatum in vivo.
abstract: Action potential (AP) patterns and dopamine (DA) release are known to correlate with rewarding behaviors, but how codes of AP bursts translate into DA release in vivo remains elusive. Here, a given AP pattern was defined by four codes, termed total AP number, frequency, number of AP bursts, and interburst time [N, f, b, i].. The 'burst effect' was calculated by the ratio (γ) of DA overflow by multiple bursts to that of a single burst when total AP number was fixed. By stimulating the medial forebrain bundle using AP codes at either physiological (20 Hz) or supraphysiological (80 Hz) frequencies, we found that DA was released from two kinetically distinct vesicle pools, the fast-releasable pool (FRP) and prolonged-releasable pool (PRP), in striatal dopaminergic terminals in vivo. We examined the effects of vesicle pools on AP-pattern dependent DA overflow and found, with given 'burst codes' [b=8, i=0.5 s], a large total AP number [N = 768, f = 80 Hz] produced a facilitating burst-effect (γ[b8/b1] = 126 ± 3%), while a small total AP number [N=96, 80 Hz] triggered a depressing-burst-effect (γ[b8/b1] = 29 ± 4%). Furthermore, we found that the PRP (but not the FRP) predominantly contributed to the facilitating-burst-effect and the FRP played an important role in the depressing-burst effect. Thus, our results suggest that striatal DA release captures pre-synaptic AP pattern information through different releasable pools.
authors.affiliation: State Key Laboratory of Membrane Bioengineering, Institute of Molecular Medicine, Peking University, Beijing, China.
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Huang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Pan-Li
authors.initials: PL
authors.lastname: Zuo
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Dou
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Xin-Jiang
authors.initials: XJ
authors.lastname: Kang
authors.forename: Hua-Dong
authors.initials: HD
authors.lastname: Xu
authors.forename: Mei-Qin
authors.initials: MQ
authors.lastname: Hu
authors.forename: Mu
authors.initials: M
authors.lastname: Jin
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Mu
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Peng
authors.forename: Claire Xi
authors.initials: CX
authors.lastname: Zhang
authors.forename: Jiu-Ping
authors.initials: JP
authors.lastname: Ding
authors.forename: Bao-Ming
authors.initials: BM
authors.lastname: Li
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000465:Algorithms; D000818:Animals; D003198:Computer Simulation; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation; D004563:Electrochemistry; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D013572:Synaptic Vesicles / Q000378:metabolism / Q000502:physiology*
chemical_list: D007473:Ion Channels; D004298:Dopamine