title: Strain differences in the neural, behavioral, and molecular correlates of sweet and salty taste in naive, ethanol- and sucrose-exposed P and NP rats.
abstract: Strain differences between naive, sucrose- and ethanol-exposed alcohol-preferring (P) and alcohol-nonpreferring (NP) rats were investigated in their consumption of ethanol, sucrose, and NaCl; chorda tympani (CT) nerve responses to sweet and salty stimuli; and gene expression in the anterior tongue of T1R3 and TRPV1/TRPV1t. Preference for 5% ethanol and 10% sucrose, CT responses to sweet stimuli, and T1R3 expression were greater in naive P rats than NP rats. The enhancement of the CT response to 0.5 M sucrose in the presence of varying ethanol concentrations (0.5-40%) in naive P rats was higher and shifted to lower ethanol concentrations than NP rats. Chronic ingestion of 5% sucrose or 5% ethanol decreased T1R3 mRNA in NP and P rats. Naive P rats also demonstrated bigger CT responses to NaCl+benzamil and greater TRPV1/TRPV1t expression. TRPV1t agonists produced biphasic effects on NaCl+benzamil CT responses, enhancing the response at low concentrations and inhibiting it at high concentrations. The concentration of a TRPV1/TRPV1t agonist (Maillard reacted peptides conjugated with galacturonic acid) that produced a maximum enhancement in the NaCl+benzamil CT response induced a decrease in NaCl intake and preference in P rats. In naive P rats and NP rats exposed to 5% ethanol in a no-choice paradigm, the biphasic TRPV1t agonist vs. NaCl+benzamil CT response profiles were higher and shifted to lower agonist concentrations than in naive NP rats. TRPV1/TRPV1t mRNA expression increased in NP rats but not in P rats exposed to 5% ethanol in a no-choice paradigm. We conclude that P and NP rats differ in T1R3 and TRPV1/TRPV1t expression and neural and behavioral responses to sweet and salty stimuli and to chronic sucrose and ethanol exposure.
authors.affiliation: Dept. of Physiology and Biophysics, Virginia Commonwealth Univ., Richmond, VA 23219, USA.
authors.forename: Jamison
authors.initials: J
authors.lastname: Coleman
authors.forename: Ashley
authors.initials: A
authors.lastname: Williams
authors.forename: Tam-Hao T
authors.initials: TH
authors.lastname: Phan
authors.forename: Shobha
authors.initials: S
authors.lastname: Mummalaneni
authors.forename: Pamela
authors.initials: P
authors.lastname: Melone
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Ren
authors.forename: Huiping
authors.initials: H
authors.lastname: Zhou
authors.forename: Sunila
authors.initials: S
authors.lastname: Mahavadi
authors.forename: Karnam S
authors.initials: KS
authors.lastname: Murthy
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Katsumata
authors.forename: John A
authors.initials: JA
authors.lastname: DeSimone
authors.forename: Vijay
authors.initials: V
authors.lastname: Lyall
mesh_terms: D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology; D000813:Anilides / Q000494:pharmacology; D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology; D002814:Chorda Tympani Nerve / Q000502:physiology; D002934:Cinnamates / Q000494:pharmacology; D019422:Dietary Sucrose / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects / Q000502:physiology*; D005518:Food Preferences / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008954:Models, Biological; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000235:genetics; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*; D013045:Species Specificity; D050916:TRPV Cation Channels / Q000819:agonists / Q000235:genetics; D055697:Taste Perception / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D014059:Tongue / Q000502:physiology
chemical_list: D000813:Anilides; D002492:Central Nervous System Depressants; D002934:Cinnamates; D019422:Dietary Sucrose; D004224:Diterpenes; C477659:N-(3-methoxyphenyl)-4-chlorocinnamanilide; D043562:Receptors, G-Protein-Coupled; D017673:Sodium Chloride, Dietary; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C431926:taste receptors, type 1; C013407:benzamil; D000431:Ethanol; D000584:Amiloride; C024353:resiniferatoxin