title: Identification of distinct amino acids as ADP-ribose acceptor sites by mass spectrometry.
abstract: ADP-ribosylation is a well-known post-translational protein modification, which regulates a variety of -cellular processes. The proteins able to catalyze mono- or poly ADP-ribosylation of proteins belong to the family of ADP-ribosyltransferases. A variety of nuclear proteins has been described to be ADP-ribosylated, including ARTD1 itself and histone proteins. Despite intensive research during the last 40 years, the acceptor amino acids in ARTD1 or histone proteins could be identified and confirmed only recently by MS/MS and by site-directed mutagenesis. The establishment of a standardized protocol including the specific enrichment of ADP-ribosylated proteins and peptides and subsequent mass spectrometric analysis allows the identification of ADP-ribose acceptor sites of modified proteins and to address the functional contribution of ADP-ribosylation in vitro as well as in vivo.
authors.affiliation: Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Zurich, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Rosenthal
authors.forename: Simon
authors.initials: S
authors.lastname: Messner
authors.forename: Bernd
authors.initials: B
authors.lastname: Roschitzki
authors.forename: Peter
authors.initials: P
authors.lastname: Gehrig
authors.forename: Paolo
authors.initials: P
authors.lastname: Nanni
authors.forename: Michael O
authors.initials: MO
authors.lastname: Hottiger
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry
chemical_list: D000596:Amino Acids; D000246:Adenosine Diphosphate Ribose; D011065:Poly(ADP-ribose) Polymerases