title: Anti-tumor and immunomodulatory activity of resveratrol in vitro and its potential for combining with cancer immunotherapy.
abstract: We evaluated the anti-tumor effect of Resveratrol (RV) on M21 and NXS2 tumor cell lines and its immunosuppressive activity on human and murine immune cells to determine the potential for combining RV and immunotherapy. In vitro, concentrations of RV≥25 mcM, inhibited cell proliferation, blocked DNA synthesis and induced G1 phase arrest in tumor and immune cells. RV at 12-50 mcM inhibited antibody dependent cell mediated cytotoxicity (ADCC) of tumor cells facilitated by the hu14.18-IL2 immunocytokine (IC). The in vivo anti-tumor and immunomodulating activity of RV given systemically were assessed in mice. Results showed that this RV regimen inhibited the growth of NXS2 tumors in vivo but did not appear to interfere with blood cell count, splenocyte or macrophage function. Thus, RV may be a candidate for combining with immunotherapy.
authors.affiliation: Department of Human Oncology, University of Wisconsin, Madison, WI 53706, USA.
authors.forename: Brenda L
authors.initials: BL
authors.lastname: Soto
authors.forename: Jacquelyn A
authors.initials: JA
authors.lastname: Hank
authors.forename: Soesiawati R
authors.initials: SR
authors.lastname: Darjatmoko
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Polans
authors.forename: Eric M
authors.initials: EM
authors.lastname: Yanke
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Rakhmilevich
authors.forename: Songwon
authors.initials: S
authors.lastname: Seo
authors.forename: KyungMann
authors.initials: K
authors.lastname: Kim
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Reisfeld
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Gillies
authors.forename: Paul M
authors.initials: PM
authors.lastname: Sondel
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D000077185:Resveratrol; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes; D000077185:Resveratrol