title: An emulsion based microarray method to detect the toxin genes of toxin-producing organisms.
abstract: Toxins produced by bacteria and fungi are one of the most important factors which may cause food contamination. The study of detection methods with high sensitivity and throughput is significant for the protection of food safety. In the present study, we coupled microarray with emulsion PCR and developed a high throughput detection method. Thirteen different gene sites which encode the common toxins of several bacteria and fungi were assayed in parallel in positive and maize samples. Conventional PCR assays were carried out for comparison. The results showed that the developed microarray method had high specificity and sensitivity. Two zearalenone-related genes were investigated in one of the ten maize samples obtained with this present method. The results indicated that the emulsion based microarray detection method was developed successfully and suggested its potential application in multiple gene site detection.
authors.affiliation: Key Laboratory of Child Development and Learning Science, Ministry of Education, Southeast University, Nanjing 210096, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Lu
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics*; D004655:Emulsions; D005516:Food Microbiology; D024282:Genes, sry; D006801:Humans; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009183:Mycotoxins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D003313:Zea mays / Q000382:microbiology
chemical_list: D001427:Bacterial Toxins; D004655:Emulsions; D009183:Mycotoxins