title: Evaluation of apoptosis-related genes: Fas (CD94), FasL (CD178) and TRAIL polymorphisms in Iranian multiple sclerosis patients.
abstract: Multiple sclerosis (MS) is a central nervous system (CNS) demyelinating disease characterized by a relapsing-remitting course leading to progressive disability. Given the critical role of apoptosis-related genes in the maintenance of homeostasis in the immune privilege sites, mutations in these genes have a profound effect on occurring autoimmune diseases such as multiple sclerosis. In the current study, polymorphisms in the apoptosis-related genes: Fas _-670 A>G, FasL _-844C>T, FasLIVS2nt _124 A>G and TRAIL_1595C>T were analyzed in 107 Iranian patients suffering from MS and 112 unrelated healthy controls using PCR-RFLP method. Our results demonstrated that among Iranian patients with MS and controls being homozygous in Fas_670A/A, G/G and FasL_-844C/C, TT in the promoter region and homozygocity in the minor allele for FasLIVS2nt_124G/G and TRAIL_1595C/C, polymorphisms were not associated with the MS risk in Iranian patients when compared with normal controls. However, the Fas _-670G/G genotype had a borderline significantly increased frequency with secondary progressive MS type (X(2)=5.8, P=0.05). In conclusion, no statistical association was found between the Fas, FasL and TRAIL polymorphisms and the risk of MS in Iranian patients.
authors.affiliation: Department of Immunology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Mohammadzadeh
authors.forename: A A
authors.initials: AA
authors.lastname: Pourfathollah
authors.forename: M A
authors.initials: MA
authors.lastname: Sahraian
authors.forename: M
authors.initials: M
authors.lastname: Behmanesh
authors.forename: S
authors.initials: S
authors.lastname: Daneshmandi
authors.forename: Z
authors.initials: Z
authors.lastname: Moeinfar
authors.forename: M
authors.initials: M
authors.lastname: Heidari
mesh_terms: D000328:Adult; D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D020528:Multiple Sclerosis, Chronic Progressive / Q000453:epidemiology / Q000235:genetics*; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D012307:Risk Factors; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics*; D019014:fas Receptor / Q000235:genetics*
chemical_list: D051017:Apoptosis Regulatory Proteins; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D019014:fas Receptor