title: Spectroscopic analysis on the resveratrol-DNA binding interactions at physiological pH.
abstract: The interaction of resveratrol with calf thymus deoxyribonucleic acid (ctDNA) under physiological conditions (Tris-HCl buffer solutions, pH 7.4) was studied by spectroscopy, fluorescence spectroscopy and viscosity measurement method, respectively. Results indicated that a complex of resveratrol with ctDNA was formed with a binding constant of K(17°C) = 5.49×10(3) L mol(-1) and K(37°C) = 1.90×10(4) L mol(-1). The fluorescence quenching mechanism of acridine orange (AO)-ctDNA by resveratrol was shown to be a static quenching type. The thermodynamic parameters of the complex were calculated by a double reciprocal method: Δ(r)H(m)(s) = 4.64×10(4)  J  mol(-1), Δ(r)S(m)(s) = 231.8  J  K(-1)  mol(-1) and Δ(r)G(m)(s) = -2.54×10(4)  J  mol(-1) (37°C). Spectroscopic techniques together with viscosity determination provided evidences of intercalation mode of binding for the interaction between resveratrol and ctDNA.
authors.affiliation: College of Chemistry and Chemical Engineering, Qufu Normal University, Shandong, Qufu 273165, People's Republic of China. zhshf999@sina.com
authors.forename: Shufang
authors.initials: S
authors.lastname: Zhang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Jing
authors.forename: Fengli
authors.initials: F
authors.lastname: Qu
mesh_terms: D000042:Absorption; D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D011485:Protein Binding; D000077185:Resveratrol; D013050:Spectrometry, Fluorescence / Q000379:methods; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013267:Stilbenes / Q000737:chemistry*; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D013267:Stilbenes; D004247:DNA; C427203:calf thymus DNA; D000077185:Resveratrol