title: Detection and characterization of receptor interactions with PDZ domains.
abstract: Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.
authors.affiliation: Department of Pharmacology, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Stefanie L
authors.initials: SL
authors.lastname: Ritter
authors.forename: Randy A
authors.initials: RA
authors.lastname: Hall
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D054731:PDZ Domains; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins