title: Expression of SOCS1-7 and CIS mRNA in porcine tissues.
abstract: The Suppressor Of Cytokine Signaling (SOCS) proteins are key physiological regulators of the immune system. Little is known about tissue expression of SOCS and data in pigs are extremely scarce. In order to further study SOCS in pigs, preliminary data must be collected. In the current report, we first identified the three most suitable reference genes in ten porcine tissues. The beta-2-microglobulin (B2MI) reference gene was most often particularly suitable in our conditions. Then, using three reference genes we determined the mRNA expression of SOCS1-7 and CIS in every selected tissue. Constitutive mRNA expression was identified for all the members of the SOCS family in the ten tissues. Interestingly, the constitutive mRNA expression of SOCS1, SOCS3, SOCS7 and CIS was rather heterogeneous between tissues while for SOCS2, SOCS4, SOCS5 and SOCS6 differences of expression were less obvious. Highest CIS and SOCS mRNA expressions were observed in large intestine (SOCS1, SOCS3, SOCS4, SOCS6, and CIS), small intestine (SOCS1, SOCS4, SOCS6, and CIS), spleen (SOCS2, SOCS3, SOCS5, SOCS7, and CIS), trachea (SOCS3) and thymus (SOCS1, SOCS2, SOCS4, SOCS7, and CIS). These data will help for further studies about the role of SOCS proteins in the control of porcine innate and adaptive responses.
authors.affiliation: Institut National de Recherche Agronomique, UR1282, Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France.
authors.forename: Mario
authors.initials: M
authors.lastname: Delgado-Ortega
authors.forename: Sandrine
authors.initials: S
authors.lastname: Melo
authors.forename: François
authors.initials: F
authors.lastname: Meurens
mesh_terms: D000818:Animals; D007420:Intestine, Large / Q000737:chemistry / Q000378:metabolism; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000737:chemistry / Q000378:metabolism; D050826:Suppressor of Cytokine Signaling Proteins / Q000032:analysis / Q000096:biosynthesis*; D013552:Swine / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000737:chemistry / Q000378:metabolism; D014018:Tissue Distribution; D014132:Trachea / Q000737:chemistry / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D050826:Suppressor of Cytokine Signaling Proteins