title: Kinetin applications alleviate salt stress and improve the antioxidant composition of leaf extracts in Salvia officinalis.
abstract: A pot experiment was carried out under glasshouse conditions with common sage (Salvia officinalis L.) to investigate the interactive effects of salt stress and kinetin on growth attributes and the abundance of pigments, ions, phenolic diterpenes and α-tocopherol in leaf extracts of this species. The plants were subjected to the following four treatments: (i) control (nutrient solution), (ii) control + 10 μM kinetin, (iii) salt stress (nutrient solution + 100 mM NaCl), and (iv) salt stress + 10 μM kinetin. Kinetin was applied as a foliar fertilizer. Salt stress reduced water contents, photosynthetic activity and pigment contents of sage leaves. In addition, it increased Na(+) contents, and reduced those of Ca(2+) and K(+) in leaves. Salt stress reduced carnosic acid and 12-O-methyl carnosic acid contents in leaves, while it did not affect carnosol and α-tocopherol contents. Foliar applications of kinetin seemed to counterbalance or alleviate the stress symptoms induced by salinity, improving ion and pigment contents, while leaf phenolic diterpene (mainly carnosol) and α-tocopherol contents also increased in both control and NaCl-treated plants; still this effect was much more obvious in salt-treated plants. A similar effect was also obtained when plants were sprayed with KNO(3) or Ca(NO(3))(2), thus suggesting that kinetin effects were at least partly due to an improvement of ion homeostasis. Kinetin applications resulted in increased transcript levels of the isoprenoid and tocopherol biosynthetic genes, DXPRI and VTE2 and VTE4 in control plants, but not in NaCl-treated plants. We conclude that kinetin can alleviate the negative impact of salt on sage plants cultivated under arid environments with salinity problems.
authors.affiliation: Laboratory of Biotechnology Applied to Crop Improvement, Faculty of Sciences of Gabes, University of Gabes, Cité Erriadh, Zrig 6072 Gabès, Tunisia. tounektico@yahoo.fr
authors.forename: Taïeb
authors.initials: T
authors.lastname: Tounekti
authors.forename: Iker
authors.initials: I
authors.lastname: Hernández
authors.forename: Maren
authors.initials: M
authors.lastname: Müller
authors.forename: Habib
authors.initials: H
authors.lastname: Khemira
authors.forename: Sergi
authors.initials: S
authors.lastname: Munné-Bosch
mesh_terms: D045784:Abietanes / Q000032:analysis / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D017610:Calcium Compounds / Q000494:pharmacology; D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D004224:Diterpenes / Q000032:analysis / Q000378:metabolism; D005308:Fertilizers; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006706:Homeostasis; D007701:Kinetin / Q000494:pharmacology*; D009566:Nitrates / Q000494:pharmacology; D010788:Photosynthesis; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism; D018526:Plant Transpiration; D017680:Potassium Compounds / Q000494:pharmacology; D018749:RNA, Plant / Q000302:isolation & purification; D055051:Salt-Tolerant Plants / Q000378:metabolism; D027543:Salvia officinalis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D014810:Vitamin E / Q000378:metabolism; D024502:alpha-Tocopherol / Q000032:analysis / Q000378:metabolism
chemical_list: D045784:Abietanes; D000975:Antioxidants; D017610:Calcium Compounds; D004224:Diterpenes; D005308:Fertilizers; D009566:Nitrates; D010936:Plant Extracts; D017680:Potassium Compounds; D018749:RNA, Plant; D014810:Vitamin E; D002734:Chlorophyll; D012965:Sodium Chloride; D024502:alpha-Tocopherol; C018381:salvin; C059948:calcium nitrate; D007701:Kinetin; C023844:potassium nitrate