title: Chromosomal localization of two novel repetitive sequences isolated from the Chenopodium quinoa Willd. genome.
abstract: The chromosomal organization of two novel repetitive DNA sequences isolated from the Chenopodium quinoa Willd. genome was analyzed across the genomes of selected Chenopodium species. Fluorescence in situ hybridization (FISH) analysis with the repetitive DNA clone 18-24J in the closely related allotetraploids C. quinoa and Chenopodium berlandieri Moq. (2n = 4x = 36) evidenced hybridization signals that were mainly present on 18 chromosomes; however, in the allohexaploid Chenopodium album L. (2n = 6x = 54), cross-hybridization was observed on all of the chromosomes. In situ hybridization with rRNA gene probes indicated that during the evolution of polyploidy, the chenopods lost some of their rDNA loci. Reprobing with rDNA indicated that in the subgenome labeled with 18-24J, one 35S rRNA locus and at least half of the 5S rDNA loci were present. A second analyzed sequence, 12-13P, localized exclusively in pericentromeric regions of each chromosome of C. quinoa and related species. The intensity of the FISH signals differed considerably among chromosomes. The pattern observed on C. quinoa chromosomes after FISH with 12-13P was very similar to GISH results, suggesting that the 12-13P sequence constitutes a major part of the repetitive DNA of C. quinoa.
authors.affiliation: Department of Plant Anatomy and Cytology, University of Silesia, Katowice, Poland. bozena.kolano@us.edu.pl
authors.forename: B
authors.initials: B
authors.lastname: Kolano
authors.forename: B W
authors.initials: BW
authors.lastname: Gardunia
authors.forename: M
authors.initials: M
authors.lastname: Michalska
authors.forename: A
authors.initials: A
authors.lastname: Bonifacio
authors.forename: D
authors.initials: D
authors.lastname: Fairbanks
authors.forename: P J
authors.initials: PJ
authors.lastname: Maughan
authors.forename: C E
authors.initials: CE
authors.lastname: Coleman
authors.forename: M R
authors.initials: MR
authors.lastname: Stevens
authors.forename: E N
authors.initials: EN
authors.lastname: Jellen
authors.forename: J
authors.initials: J
authors.lastname: Maluszynska
mesh_terms: D001483:Base Sequence; D027465:Chenopodium quinoa / Q000235:genetics*; D032461:Chromosomes, Plant / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D015723:Gene Library; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal