title: Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii.
abstract: Grain hardness of wheat is determined by the hardness (Ha)-locus region, which contains three friabilin-related genes: puroindoline-a (Pina), puroindoline-b (Pinb) and GSP-1. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha-locus region of Aegilops tauschii containing Pina and GSP-1 genes by Agrobacterium-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP-1, were stably expressed in endosperms of the T3 and T4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds.
authors.affiliation: Division of Natural Science, Osaka Kyoiku University, 4-698-1 Asahigaoka, Kashiwara, Osaka, 582-8582, Japan. gsuzuki@cc.osaka-kyoiku.ac.jp
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.forename: Hideo
authors.initials: H
authors.lastname: Wada
authors.forename: Hiromi
authors.initials: H
authors.lastname: Goto
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakano
authors.forename: Haruna
authors.initials: H
authors.lastname: Oba
authors.forename: Takuya
authors.initials: T
authors.lastname: Deno
authors.forename: Sadequr
authors.initials: S
authors.lastname: Rahman
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Mukai
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056625:Endosperm / Q000648:ultrastructure; D017343:Genes, Plant; D005822:Genetic Vectors; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000235:genetics; D014170:Transformation, Genetic; D019076:Transgenes; D014908:Triticum / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; C088852:GSP-1 protein, Triticum aestivum; D010940:Plant Proteins; C036483:T-DNA