title: Polymeric sequence probe for single DNA detection.
abstract: A polynucleotide probe, polymeric sequence probe (PSP), was developed for single molecular detections. PSP is a single-stranded DNA molecule with ~2000 tandem repeat target-binding sequences and label-binding sequences. A single PSP can bind to multiple fluorescent complementary oligos to generate a strong fluorescence signal. Single target molecules bound to PSPs can be clearly visualized by a conventional fluorescence microscope. An ultrasensitive PSP-based assay for Mycobacterium tuberculosis was demonstrated.
authors.forename: Suxian
authors.initials: S
authors.lastname: Huang
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D015342:DNA Probes / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000235:genetics; D011119:Polynucleotides / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D004277:DNA, Single-Stranded; D011119:Polynucleotides