title: PaCATB, a secreted catalase protecting Podospora anserina against exogenous oxidative stress.
abstract: A differential mass spectrometry analysis of secreted proteins from juvenile and senescent Podospora anserina cultures revealed age-related differences in protein profiles. Among other proteins with decreased abundance in the secretome of senescent cultures a catalase, termed PaCATB, was identified. Genetic modulation of the abundance of PaCATB identified differential effects on the phenotype of the corresponding strains. Deletion of PaCatB resulted in decreased resistance, over-expression in increased resistance against hydrogen peroxide. While the lifespan of the genetically modified strains was found to be unaffected under standard growth conditions, increased exogenous hydrogen peroxide stress in the growth medium markedly reduced the lifespan of the PaCatB deletion strain but extended the lifespan of PaCatB over-expressors. Overall our data identify a component of the secretome of P. anserina as a new effective factor to cope with environmental stress, stress that under natural conditions is constantly applied on organisms and influences aging processes.
authors.affiliation: Institute of Molecular Biosciences and 'Cluster of Excellence Macromolecular Complexes', Department of Biosciences, J.W. Goethe-University, Frankfurt am Main, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Zintel
authors.forename: Dominik
authors.initials: D
authors.lastname: Bernhardt
authors.forename: Adelina
authors.initials: A
authors.lastname: Rogowska-Wrzesinska
authors.forename: Heinz D
authors.initials: HD
authors.lastname: Osiewacz
mesh_terms: D000375:Aging / Q000502:physiology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D016681:Genome, Fungal; D006861:Hydrogen Peroxide / Q000633:toxicity; D030781:Organisms, Genetically Modified; D018384:Oxidative Stress / Q000502:physiology*; D048468:Podospora / Q000187:drug effects / Q000201:enzymology*
chemical_list: D006861:Hydrogen Peroxide; D002374:Catalase