title: The antioxidant effect of wheat germ oil on subchronic coumaphos exposure in mice.
abstract: Forty-eight male Balb/C mice, allocated to 4 equal groups, constituted the material of the study. The first group was maintained as the control group and was administered solely with a vehicle, which was used to dissolve coumaphos in the third and fourth groups. The second group was administered with 1.5 ml/kg.bw/day (∼1400 mg/kg.bw/day) of wheat germ oil. The third group received 5.5mg/kg.bw/day (1/10 LD50(oral)) of coumaphos. Finally, the fourth group was given both coumaphos and wheat germ oil at the doses indicated above. In all groups, the compounds were given directly into the stomach using a gastric tube, and treatment was continued for a period of 45 days. At the end of the 45th day, the liver, lungs, kidneys, brain, heart and spleen were extirpated in all of the animals. Tissue homogenates prepared from the tissue specimens were analysed for malondialdehyde (MDA) levels and catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. In conclusion, it was determined that coumaphos led to adverse alterations in the majority of the oxidative stress markers investigated. The administration of wheat germ oil alleviated the coumpahos-induced adverse effects detected in the tissues examined.
authors.affiliation: Erciyes University, Safiye Çıkrıkçıoğlu Vocational College, Department of Animal Science, Kayseri, Turkey.
authors.forename: Mürsel
authors.initials: M
authors.lastname: Karabacak
authors.forename: Murat
authors.initials: M
authors.lastname: Kanbur
authors.forename: Gökhan
authors.initials: G
authors.lastname: Eraslan
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Soyer Sarıca
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003372:Coumaphos / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007306:Insecticides / Q000633:toxicity; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D010938:Plant Oils / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007306:Insecticides; D010938:Plant Oils; C050055:wheat germ oil; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003372:Coumaphos