title: Adenosine and ATP affect LPS-induced cytokine production in canine macrophage cell line DH82 cells.
abstract: Macrophages are essential for controlling the majority of infections, and are mediators of natural immunity. During infection, lipopolysaccharide (LPS) stimulates macrophages to produce pro-inflammatory cytokines. Adenosine and ATP released into the extracellular space by immunological stimuli have been shown to regulate various immune functions. More recently, it has been shown adenosine and ATP have a critical role on the physiological negative feedback mechanism for limitation and termination of tissue-specific and systemic inflammatory responses. It was useful and meaningful to gain information about interaction between LPS, which generates the inflammation, and adenosine and ATP, which terminate the inflammation. We evaluate effects of adenosine and ATP on the production of cytokines related to inflammation in canine macrophage cell line DH82 cells. Adenosine and ATP respectively increased the production of IL-10 without affecting the production of IL-6, TNF-α and IL-12 in DH82 cells. In addition, adenosine and ATP prevented the production of LPS-induced IL-6, TNF-α and IL-12 in DH82 cells. In contrast, adenosine and ATP potentiated LPS-induced IL-10 production in DH82 cells. Moreover, adenosine, but not ATP inhibited LPS-induced expression of TLR4 in DH82 cells. These results suggest that conditions related to increased adenosine and/or ATP may play an important role in the inflammatory reactions.
authors.affiliation: Laboratory of Advanced Diagnosis and Treatment, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Izumisano, Osaka, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: Naoko
authors.initials: N
authors.lastname: Nakatani
authors.forename: Takeya
authors.initials: T
authors.lastname: Kubo
authors.forename: Yuko
authors.initials: Y
authors.lastname: Semi
authors.forename: Natsuho
authors.initials: N
authors.lastname: Yoshida
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Nakajima
authors.forename: Toshie
authors.initials: T
authors.lastname: Iseri
authors.forename: Yasu-Taka
authors.initials: YT
authors.lastname: Azuma
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D005786:Gene Expression Regulation; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D000255:Adenosine Triphosphate; D000241:Adenosine