title: Exploration of the relationship between the tumor burden and the concentration of vascular endothelial growth factor in liver-cancer-bearing animals using immunomagnetic reduction assay.
abstract: To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
authors.affiliation: Department of Surgery and Hepatitis Research Center National Taiwan University Hospital, National Taiwan University, Taipei 100, Taiwan.
authors.forename: K W
authors.initials: KW
authors.lastname: Huang
authors.forename: S Y
authors.initials: SY
authors.lastname: Yang
authors.forename: C Y
authors.initials: CY
authors.lastname: Yu
authors.forename: J J
authors.initials: JJ
authors.lastname: Chieh
authors.forename: Che-Chuan
authors.initials: CC
authors.lastname: Yang
authors.forename: Herng-Er
authors.initials: HE
authors.lastname: Horng
authors.forename: Chin-Yih
authors.initials: CY
authors.lastname: Hong
authors.forename: Hong-Chang
authors.initials: HC
authors.lastname: Yang
authors.forename: Chau-Chung
authors.initials: CC
authors.lastname: Wu
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D008114:Liver Neoplasms, Experimental / Q000097:blood* / Q000473:pathology*; D008280:Magnetics / Q000379:methods; D058185:Magnetite Nanoparticles; D008297:Male; D036103:Nanotechnology; D051381:Rats; D017208:Rats, Wistar; D047368:Tumor Burden; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D058185:Magnetite Nanoparticles; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat