title: Negligible penetration of incidental amounts of alpha-hydroxy acid from rinse-off personal care products in human skin using an in vitro static diffusion cell model.
abstract: Alpha-hydroxy acids (AHAs), primarily glycolic and lactic acids, are widely used in cosmetics to alleviate dyspigmentation, photodamage, and other aging skin conditions and as pH adjusters. Glycolic acid reportedly enhances skin damage after repeated ultraviolet light exposure, e.g., increased sunburn cell formation. This study assessed potential in vitro skin penetration of lactic acid and malic acid incorporated into rinse-off personal care products, compared with rinse-off and leave-on exposures to glycolic acid (10%, pH 3.5) in a reference lotion. Radiolabeled AHA-fortified shampoo, conditioner, and lotion were evenly applied as single doses to human epidermal membranes mounted in static diffusion cells (not occluded). Exposures were 1-3 min (rinse-off) or 24 h (leave-on). Epidermal penetration of malic acid and lactic acid from the rinse-off shampoo and conditioner, respectively, was negligible, with >99% removed by rinsing, a negligible portion remaining in the stratum corneum (≤0.15%), and even less penetrating into the viable epidermis (≤0.04%). Glycolic acid penetration from the leave-on reference lotion was 1.42 μg equiv./cm2/h, with total absorbable dose recovery (receptor fluid plus epidermis) of 2.51%, compared to 0.009%, 0.003%, and 0.04% for the rinse-off reference lotion, shampoo (malic acid), and conditioner (lactic acid) exposures, respectively. Dermal penetration of AHAs into human skin is pH-, concentration-, and time-dependent. Alpha-hydroxy acids in rinse-off shampoos and conditioners are almost entirely removed from the skin within minutes by rinsing (resulting in negligible epidermal penetration). This suggests that ultraviolet radiation-induced skin effects of AHA-containing rinse-off products are negligible.
authors.affiliation: Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okuda
authors.forename: D A
authors.initials: DA
authors.lastname: Donahue
authors.forename: L E
authors.initials: LE
authors.lastname: Kaufman
authors.forename: J
authors.initials: J
authors.lastname: Avalos
authors.forename: F A
authors.initials: FA
authors.lastname: Simion
authors.forename: D C
authors.initials: DC
authors.lastname: Story
authors.forename: H
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: R
authors.initials: R
authors.lastname: Fautz
authors.forename: A
authors.initials: A
authors.lastname: Fuchs
mesh_terms: D004817:Epidermis / Q000737:chemistry / Q000378:metabolism*; D006016:Glycolates / Q000737:chemistry / Q000378:metabolism; D006203:Hair Preparations / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008293:Malates / Q000737:chemistry / Q000378:metabolism*; D012869:Skin Absorption; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D006016:Glycolates; D006203:Hair Preparations; D008293:Malates; D014867:Water; C031149:glycolic acid; D019344:Lactic Acid; C030298:malic acid