title: Induction of insert-specific immune response in mice by hamster polyomavirus VP1 derived virus-like particles carrying LCMV GP33 CTL epitope.
abstract: Hamster polyomavirus (HaPyV) major capsid protein VP1 based chimeric virus-like particles (VLPs) carrying model GP33 CTL epitope derived from Lymphocytic choriomeningitis virus (LCMV) were generated in yeast and examined for their capability to induce CTL response in mice. Chimeric VP1-GP33 VLPs were effectively processed in antigen presenting cells in vitro and in vivo and induced antigen-specific CD8+ T cell proliferation. Mice immunized only once with VP1-GP33 VLPs without adjuvant developed an effective GP33-specific memory T cell response: 70% were fully and 30% partially protected from LCMV infection. Moreover, aggressive growth of tumors expressing GP33 was significantly delayed in these mice in vivo. Therefore, HaPyV VP1-derived VLP harboring CTL epitopes are attractive vaccine candidates for the induction of insert-specific CTL immune response.
authors.affiliation: Vilnius University, Institute of Biotechnology, Graiciuno 8, LT-02241 Vilnius, Lithuania.
authors.forename: Egle
authors.initials: E
authors.lastname: Mazeike
authors.forename: Alma
authors.initials: A
authors.lastname: Gedvilaite
authors.forename: Ulrike
authors.initials: U
authors.lastname: Blohm
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001117:Arenaviridae Infections / Q000276:immunology / Q000517:prevention & control; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics*; D049109:Cell Proliferation; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D004337:Drug Carriers; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology / Q000472:pathogenicity; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology*; D011120:Polyomavirus / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D022721:Vaccines, Virosome / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; D004337:Drug Carriers; D018984:Epitopes, T-Lymphocyte; D006023:Glycoproteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C062481:VP1 protein, polyomavirus; D022721:Vaccines, Virosome; D014764:Viral Proteins; D014765:Viral Vaccines; C094244:glycoprotein peptide 33-41, Lymphocytic choriomeningitis virus