title: NY-BR-1 and PAX8 immunoreactivity in breast, gynecologic tract, and other CK7+ carcinomas: potential use for determining site of origin.
abstract: The distinction between breast and müllerian carcinomas from each other and from tumors with a similar cytokeratin profile can be difficult. We tested the usefulness of 2 new markers, NY-BR-1 and PAX8, by staining a variety of breast and gynecologic carcinomas, along with tumors of pancreas, bile ducts, stomach, and gastroesophageal junction. NY-BR-1 expression (ie, H score >10) was seen in 58.4% of breast carcinomas (111/190), 5.6% of müllerian carcinomas (8/142), 7% of pancreatic tumors (1/15), 0% of cholangiocarcinomas (0/22), 0% of gastric tumors (0/36), and 0% of gastroesophageal carcinomas (0/25). All 188 breast carcinomas were negative for PAX8. PAX8 expression was seen in 72.4% of müllerian tumors (105/145). All pancreatic tumors (n = 15), cholangiocarcinomas (n = 23), and gastric (n = 35) and gastroesophageal junction (n = 25) carcinomas were negative for PAX8. Addition of NY-BR-1 and PAX8 in a panel would be useful in distinguishing breast cancer, gynecologic tumors, and tumors of the upper gastrointestinal tract.
authors.affiliation: Dept of Pathology, Magee-Womens Hospital, University of Pittsburgh Medical Center, PA 15213, USA.
authors.forename: Anna H
authors.initials: AH
authors.lastname: Woodard
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: David J
authors.initials: DJ
authors.lastname: Dabbs
authors.forename: Sushil
authors.initials: S
authors.lastname: Beriwal
authors.forename: Anca V
authors.initials: AV
authors.lastname: Florea
authors.forename: Esther
authors.initials: E
authors.lastname: Elishaev
authors.forename: Jon M
authors.initials: JM
authors.lastname: Davison
authors.forename: Alyssa M
authors.initials: AM
authors.lastname: Krasinskas
authors.forename: Rohit
authors.initials: R
authors.lastname: Bhargava
mesh_terms: D000328:Adult; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D002277:Carcinoma / Q000175:diagnosis* / Q000378:metabolism; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000378:metabolism*
chemical_list: C509866:ANKRD30A protein, human; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D051761:Paired Box Transcription Factors