title: Increased expression of insulin-like growth factor-1 receptor is correlated with tumor metastasis and prognosis in patients with osteosarcoma.
abstract: BACKGROUND
  The aim of this study was to investigate the association of insulin-like growth factor-1 receptor (IGF-1R) with metastasis and prognosis of osteosarcoma patients.
  
  
  METHODS
  RT-PCR and Western blot assays were performed to detect IGF-1R mRNA and protein expression in 26 osteosarcoma and noncancerous bone tissues. Immunohistochemistry was performed to analyze the correlation of IGF-1R expression in 84 osteosarcoma tissues with clinicopathological factors or survival of patients. Lentivirus-mediated RNA interference system was employed to downregulate IGF-1R expression and analyze the effects of IGF-1R downregulation on invasion and metastasis of osteosarcoma cells.
  
  
  RESULTS
  The relative levels of IGF-1R mRNA and protein expression were significantly higher in osteosarcoma tissues than in corresponding noncancerous bone tissues. The expression of IGF-1R protein was closely associated with surgical stage and distant metastasis of osteosarcoma patients. Osteosarcoma patients with high IGF-1R expression had poorer survival, and multivariate Cox analyses showed that high IGF-1R expression was an independent prognostic maker. Lentivirus-mediated targeting IGF-1R could significantly inhibit adhesion, migration, invasion, and metastasis of osteosarcoma cells, which might be correlated with of inactivation of Akt signaling pathway.
  
  
  CONCLUSIONS
  IGF-1R is an independent prognostic marker for osteosarcoma patients and increased expression of this molecular is correlated with metastasis of osteosarcoma.
authors.affiliation: Department of Orthopaedic Surgery, The Affiliated Drum Tower Hospital of Nanjing, University Medical School, Nanjing, Jaingsu, P.R. China.
authors.forename: Yin-He
authors.initials: YH
authors.lastname: Wang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiu
authors.forename: Jin
authors.initials: J
authors.lastname: Xiong
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Ze-Zhang
authors.initials: ZZ
authors.lastname: Zhu
authors.forename: Bang-Ping
authors.initials: BP
authors.lastname: Qian
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Chen
authors.forename: Shou-Feng
authors.initials: SF
authors.lastname: Wang
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Shi
authors.forename: Xu
authors.initials: X
authors.lastname: Sun
mesh_terms: D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D001859:Bone Neoplasms / Q000378:metabolism / Q000401:mortality* / Q000473:pathology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D007150:Immunohistochemistry; D016086:Lentivirus / Q000235:genetics; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000378:metabolism / Q000401:mortality* / Q000473:pathology*; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014161:Transduction, Genetic
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017526:Receptor, IGF Type 1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9