title: Distribution of human papillomavirus genotypes in archival cervical tissue from women with cervical cancer in urban Sri Lanka.
abstract: OBJECTIVE
  To identify the contributions of various human papillomavirus (HPV) genotypes in tissue samples from women diagnosed with cervical cancer in Sri Lanka.
  
  
  METHODS
  In a retrospective study, archival cervical tissues samples (n=108) obtained from Sri Lankan women diagnosed with histologically proven invasive squamous cell carcinoma between 2006 and 2007 were tested for HPV. Genotyping of HPV DNA was performed using an INNO-LiPA assay.
  
  
  RESULTS
  Overall, 93% of tumor samples tested positive for HPV DNA. HPV types 16 and 18 accounted collectively for 83.4% of the positive samples.
  
  
  CONCLUSIONS
  The findings suggest that the HPV genotypes responsible for causing cervical cancer in Sri Lanka are similar to those reported elsewhere worldwide. Consequently, women in Sri Lanka could benefit from currently available prophylactic HPV vaccines should they be implemented.
authors.affiliation: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia. nirma.samarawickrema@monash.edu
authors.forename: Nirma A
authors.initials: NA
authors.lastname: Samarawickrema
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
authors.forename: Janaki
authors.initials: J
authors.lastname: Hewavisenthi
authors.forename: Trishe
authors.initials: T
authors.lastname: Leong
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D053918:Papillomavirus Vaccines / Q000627:therapeutic use; D012189:Retrospective Studies; D013188:Sri Lanka / Q000453:epidemiology; D014505:Urban Population; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000517:prevention & control / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D053918:Papillomavirus Vaccines