title: Network-wide integration of stem cell-derived neurons and mouse cortical neurons using microfabricated co-culture devices.
abstract: Regeneration of damaged central nervous systems (CNS) is an important topic in neuroscience and neuroengineering. Grafting new neurons derived from pluripotent stem cells into damaged regions can be done to restore functions after injury. Little is known, however, about network-wide interactions between stem-cell-derived neurons and CNS neurons. In this study, we developed a co-culture method of stem cell-derived neuronal networks and CNS networks and observed spontaneous activity in the co-culture samples. By using a microfabricated poly(dimethylsiloxane) device having two culture compartments and 20 connecting microconduits, we are able to compartmentalize P19-derived neurons and mouse cortical neurons and connect them via the microconduits. Furthermore, we combined the co-culture device and a microelectrode array (MEA)-based recording system and recorded spontaneous activity in the co-cultured networks. We found that periodic synchronized bursting spreading over both neuronal networks occurred during the second week in vitro and that P19-derived neurons in the co-cultured networks had different developmental processes compared with those grown in monoculture. These findings suggest that functional interactions form between P19-dervived neurons and mouse cortical neurons and that the co-culture method is useful for exploring the network-wide integrations between stem cell-derived neurons and CNS neurons.
authors.affiliation: Department of Information Physics and Computing, Graduate School of Information Sciences and Technology, University of Tokyo, Hongo, Bunkyo-ku, Japan. yuzo_takayama@ipc.i.u-tokyo.ac.jp
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Takayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Moriguchi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Kotani
authors.forename: Takafumi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Mabuchi
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Jimbo
mesh_terms: D000200:Action Potentials; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002490:Central Nervous System / Q000166:cytology* / Q000254:growth & development; D018920:Coculture Techniques / Q000379:methods; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D054278:Embryonal Carcinoma Stem Cells / Q000166:cytology*; D051379:Mice; D008839:Microelectrodes; D055616:Microtechnology; D023421:Models, Animal; D009415:Nerve Net / Q000166:cytology* / Q000254:growth & development; D009474:Neurons / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology; D014407:Tumor Cells, Cultured
chemical_list: D004129:Dimethylpolysiloxanes; C013830:baysilon