title: Collection and propagation methods for mesenchymal stromal cells.
abstract: Mesenchymal stromal cells (MSC) are derived from adult mesenchymal tissues and have the ability to undergo differentiation into bone, cartilage, and fat, and have therefore attracted great interest in regenerative medicine. Many isolation and culture methods have been described, making comparison between laboratories and quality-control protocols difficult. A uniform protocol to characterize equine MSC has recently been proposed, aiming to introduce consistency across the equine stem cell research field. This article reviews the published techniques for collection and propagation of equine MSC, focusing on bone marrow-derived and adipose-derived cells.
authors.affiliation: Department of Veterinary Clinical Sciences, University of Edinburgh, Dick Vet Equine Hospital, Easter Bush Vet Centre, Roslin, Midlothian, EH25 9RG, UK. Sarah.e.taylor@ed.ac.uk
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Taylor
authors.forename: Peter D
authors.initials: PD
authors.lastname: Clegg
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D006736:Horses / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D013048:Specimen Handling