title: Preparation of novel anti-ski monoclonal antibodies.
abstract: Ski is an avian sarcoma virus oncogene homolog best known for inhibiting TGF beta signaling through its association with the SMAD proteins. Anti-Ski antibodies (MAbs) of high titer were prepared by immunizing BALB/c mice with multifocal intradermal injections and fusing high titer antibody producing spleen cells with myeloma cells of SP2/0 origin. Three MAbs were selected for further characterization as classes and subclasses. Antibodies were produced by these three clones with high affinities ranging from 10(9) to 10(11)/m. These clones were found to be of the immunoglobulin IgG1 and IgG2b subclass with kappa light chain. They could recognize Ski as determined by Western blot analysis. The produced MAbs will be a useful tool for further investigation of Ski functions in organisms.
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education of China, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ye
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Yang
authors.forename: Huai-Dong
authors.initials: HD
authors.lastname: Hu
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Tong
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Cai
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Zhang
authors.forename: Peng
authors.initials: P
authors.lastname: Hu
authors.forename: Sang-Lin
authors.initials: SL
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Ren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000915:Antibody Affinity; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D004926:Escherichia coli; D005786:Gene Expression Regulation; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; C060614:SKI protein, human