title: Production of laccase and decolouration of the textile dye Remazol Brilliant Blue R in temporary immersion bioreactors.
abstract: Laccase production by Trametes pubescens grown on sunflower-seed shells (SS) under solid-state fermentation (SFF) conditions in temporary immersion bioreactors was studied. Three immersion cycles were considered: 1 min immersed and 9 min non-immersed, 1 min immersed and 30 min non-immersed and 1 min immersed and 60 min non-immersed. The latter led to the highest laccase activities (4000-6000 Ul(-1)). Also, the in vitro and in vivo decolouration of the recalcitrant textile dye Remazol Brilliant Blue R (RBBR) was assessed. It was found that RBBR (133.33 mg l(-1)) was efficiently decolourised by T. pubencens grown on SS under SSF conditions in temporary immersion bioreactors in five successive batches. The percentage of RBBR decolouration was higher than 55% in 4h and around 70% in 24h in all the batches. However, it was found that RBBR decolouration by the crude culture filtrates was more advantageous. Thus, an RBBR decolouration percentage of nearly 80% in 2h was obtained.
authors.affiliation: CEIT, Unit of Environmental Engineering, Paseo Manuel de Lardizábal 15, 20018 San Sebastian, Spain. srodriguez@ceit.es
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Couto
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D019149:Bioreactors; D003116:Color; D042845:Laccase / Q000096:biosynthesis*; D055454:Trametes / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D042845:Laccase; C024758:Remazol Brilliant Blue R