title: Effect of chromatographic conditions on liquid chromatographic chiral separation of terbutaline and salbutamol on Chirobiotic V column.
abstract: In this study, a method for enantioseparation of terbutaline and salbutamol was established using Chirobiotic V column as a stationary phase. Polar ionic mode applying mobile phase containing ammonium nitrate in 100% ethanol, pH 5.1 was found to give the best separation. The salt concentration in the mobile phase and pH value were found to be the most important chromatographic factors affecting separation. Separation of enantiomers of these two basic analytes was complete in less than 10 min without applying ammonium trifluoroacetate (ATFA) or triethylamine (TEA) salts.
authors.affiliation: Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Zagazig University, Egypt.
authors.forename: Hisham
authors.initials: H
authors.lastname: Hashem
authors.forename: Clemens
authors.initials: C
authors.lastname: Tründelberg
authors.forename: Omer
authors.initials: O
authors.lastname: Attef
authors.forename: Thomas
authors.initials: T
authors.lastname: Jira
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000737:chemistry* / Q000302:isolation & purification; D000420:Albuterol / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D013237:Stereoisomerism; D013726:Terbutaline / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D013726:Terbutaline; D000420:Albuterol