title: Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
abstract: We establish a novel method for the induction and collection of mesenchymal stem cells using a typical cell surface marker, CD105, through adipogenesis from mouse ES cells. ES cells were cultured in a medium for adipogenesis. Mesenchymal stem cells from mouse ES cells were easily identified by the expression of CD105, and were isolated and differentiated into multiple mesenchymal cell types. Mesenchymal stem cells showed remarkable telomerase activity and sustained their growth for a long time with a high potential for differentiation involving skeletal myogenesis in vitro. When mesenchymal stem cells were transplanted into the injured tibialis anterior muscles, they differentiated into skeletal muscle cells in vivo. In addition, they improved the vascular formation, but never formed teratoma for longer than 6 months. Gene expression profiles revealed that mesenchymal stem cells lost pluripotency, while they acquired high potential to differentiate into mesenchymal cell lines. They thus indicate a promising new source of cell-based therapy without teratoma formation.
authors.affiliation: Department of Health Sciences, Nagoya University Graduate School of Medicine, Higashi-ku, Nagoya, Japan
authors.forename: Nana
authors.initials: N
authors.lastname: Ninagawa
authors.forename: Rumi
authors.initials: R
authors.lastname: Murakami
authors.forename: Eri
authors.initials: E
authors.lastname: Isobe
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Shigeko
authors.initials: S
authors.lastname: Torihashi
mesh_terms: D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D000071063:Endoglin; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D019098:Telomerase / Q000378:metabolism*
chemical_list: D000071063:Endoglin; C502004:Eng protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D019098:Telomerase