title: Differential mRNA translation in Medicago truncatula accessions with contrasting responses to ozone-induced oxidative stress.
abstract: Acute ozone is a model abiotic elicitor of oxidative stress and a useful tool for understanding biochemical and molecular events during oxidative signaling. Two Medicago truncatula accessions with contrasting responses to ozone were used to examine translational regulation during ozone stress. In ozone-resistant JE154, significant reduction in ribosome loading was observed within one hour of ozone treatment, suggesting energy homeostasis as a vital factor for oxidative stress management. Polysomal RNA-based expression profiling with Affymetrix arrays revealed extensive changes in the translatomes of both accessions. Messenger RNAs with low GC content in their 5' and 3'-UTRs were preferentially associated with polysomes during oxidative stress. Genebins analysis revealed extensive changes in various gene ontologies in both accessions. Extensive changes in nicotinate and nicotinamide metabolism genes were corroborated with increased levels of NAD(+) and NADH in JE154. The significantly lower NAD(+):NADH redox status in JE154, in conjunction with higher ATP amounts, provided a cellular milieu conducive for overcoming oxidative stress. Low levels of ATP, NADH, and suppression of antioxidant defense responses, abet build-up of ozone-derived ROS and ultimately lead to oxidative cell death in Jemalong.
authors.affiliation: 246 Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Puckette
authors.forename: Niranjani J
authors.initials: NJ
authors.lastname: Iyer
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Tang
authors.forename: Xin-Bin
authors.initials: XB
authors.lastname: Dai
authors.forename: Patrick
authors.initials: P
authors.lastname: Zhao
authors.forename: Ramamurthy
authors.initials: R
authors.lastname: Mahalingam
mesh_terms: D004734:Energy Metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D046913:Medicago truncatula / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017382:Reactive Oxygen Species; D010126:Ozone