title: Genetically engineered human neural stem cells with rabbit carboxyl esterase can target brain metastasis from breast cancer.
abstract: Neural stem cells (NSCs) led to the development of a novel strategy for delivering therapeutic genes to tumors. NSCs expressing rabbit carboxyl esterase (F3.CE), which activates CPT-11, significantly inhibited the growth of MDA-MB-435 cells in the presence of CPT-11. F3.CE cells migrated selectively into the brain metastases located in the opposite hemisphere. The treatment also significantly decreased tumor volume in immune-deficient mice bearing MDA-MB-435 tumors when F3.CE cells were transplanted into the contralateral hemisphere. The survival rate was significantly prolonged with the treatment with F3.CE and CPT-11. This strategy may be considered as an effective treatment regimen for brain metastases.
authors.affiliation: Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Ho Jun
authors.initials: HJ
authors.lastname: Seol
authors.forename: Juyoun
authors.initials: J
authors.lastname: Jin
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Seong
authors.forename: Kyeung Min
authors.initials: KM
authors.lastname: Joo
authors.forename: Wonyoung
authors.initials: W
authors.lastname: Kang
authors.forename: Heekyoung
authors.initials: H
authors.lastname: Yang
authors.forename: Jandi
authors.initials: J
authors.lastname: Kim
authors.forename: Chul Soo
authors.initials: CS
authors.lastname: Shin
authors.forename: Yonghyun
authors.initials: Y
authors.lastname: Kim
authors.forename: Kang Ho
authors.initials: KH
authors.lastname: Kim
authors.forename: Doo-Sik
authors.initials: DS
authors.lastname: Kong
authors.forename: Jung-Ii
authors.initials: JI
authors.lastname: Lee
authors.forename: Karen S
authors.initials: KS
authors.lastname: Aboody
authors.forename: Hong Jun
authors.initials: HJ
authors.lastname: Lee
authors.forename: Seung U
authors.initials: SU
authors.lastname: Kim
authors.forename: Do-Hyun
authors.initials: DH
authors.lastname: Nam
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000556:secondary / Q000628:therapy*; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D043182:Carboxylesterase / Q000235:genetics*; D005260:Female; D041722:Genes, Transgenic, Suicide; D005818:Genetic Engineering / Q000379:methods; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000637:transplantation*; D011817:Rabbits
chemical_list: D043182:Carboxylesterase