title: [Human platelet lysates promotes the proliferation of mesenchymal stem cells in vitro].
abstract: OBJECTIVE
  To investigate the effect of human platelet lysates (HPL) obtained from platelet-rich plasma on the proliferation and biological characteristics of human mesenchymal stem cells (MSCs) in vitro.
  
  
  METHODS
  HPL was obtained by repeated freeze-thawing of human plateletes, and the MSCs separated by density gradient centrifugation from 6 donors were expanded in medium supplemented with 10% fetal bovine serum (FCS) or HPL at different concentrations. The optimal concentration of HPL for cells culture was determined according to the cell proliferation kinetics. The cultured MSCs were characterized for their proliferation, cell phenotype, and cell cycle distribution.
  
  
  RESULTS
  The HPL-supplemented medium contained 4 essential growth factors for the growth of MSCs, namely platelet-derived growth factors (0.53∓0.06 ng/ml), basic fibroblast growth factor (37.5∓4.31 pg/ml), insulin-like growth factor-1 (0.15∓0.06 mg/ml) and transforming growth factor (5150∓463 pg/ml). Cultured in the presence of HPL at the optimal concentration of 7.5%, the MSCs displayed a spindle-shaped fibroblast-like morphology without obvious changes in the proliferation activity till passage 8 (P>0.05), similar to those of cells in FCS-supplemented culture medium. Flow cytometry and cell cycle analysis revealed no differences in the phenotypes or cell cycle distribution between the cells cultured in the presence of 7.5% HPL and 10% FCS.
  
  
  CONCLUSIONS
  The culture medium supplemented by 7.5% HPL can promote the expansion of human MSCs and maintain the basic biological characteristics of the cells.
authors.affiliation: Institute of Blood Transfusion, Guangzhou Blood Center, Guangzhou, China. leng868@163.com
authors.forename: Ru
authors.initials: R
authors.lastname: Xu
authors.forename: Wen-jie
authors.initials: WJ
authors.lastname: Xia
authors.forename: Xia
authors.initials: X
authors.lastname: Rong
authors.forename: Xin
authors.initials: X
authors.lastname: Ye
authors.forename: Yuan
authors.initials: Y
authors.lastname: Shao
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Luo
authors.forename: Yong-shui
authors.initials: YS
authors.lastname: Fu
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D002457:Cell Extracts / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology
chemical_list: D002457:Cell Extracts; D003470:Culture Media; D010982:Platelet-Derived Growth Factor; C095477:platelet-derived growth factor AB; D016222:Fibroblast Growth Factor 2