title: CsICE1 and CsCBF1: two transcription factors involved in cold responses in Camellia sinensis.
abstract: C-repeat/dehydration-responsive element binding factors (CBFs) can induce the expression of a suite of cold-responsive genes to increase plant cold tolerance, and inducer of CBF expression 1 (ICE1) is a major activator for CBF. In the present study, we isolated the full-length cDNAs of ICE1 and CBF from Camellia sinensis, designated as CsICE1 and CsCBF1, respectively. The deduced protein CsICE1 contains a highly conserved basic helix-loop-helix (bHLH) domain and C-terminal region of ICE1-like proteins. CsCBF1 contains all conserved domains of CBFs in other plant species and can specifically bind to the C-repeat/dehydration-responsive element (CRT/DRE) as confirmed by electrophoretic mobility shift assay. The transcription of CsICE1 had no apparent alteration after chilling treatment (4°C). CsCBF1 expression was not detected in normal temperature (20°C) but was induced immediately and significantly by low temperature (4°C). Our results suggest that ICE1-CBF cold-response pathway is conserved in tea plants. CsICE1 and CsCBF1, two components of this pathway, play roles in cold responses in tea plants.
authors.affiliation: Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agriculture University, Hefei, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Jiang
authors.forename: Ye-Yun
authors.initials: YY
authors.lastname: Li
authors.forename: Chao-Ling
authors.initials: CL
authors.lastname: Wei
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Deng
mesh_terms: D000064:Acclimatization / Q000502:physiology; D000595:Amino Acid Sequence; D001665:Binding Sites; D028241:Camellia sinensis / Q000502:physiology*; D003080:Cold Temperature; D017124:Conserved Sequence; D018076:DNA, Complementary; D024202:Electrophoretic Mobility Shift Assay; D004926:Escherichia coli / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D020218:Response Elements; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D014157:Transcription Factors