title: Electrochemical identification of artificial oligonucleotides related to bovine species. Potential for identification of species based on mismatches in the mitochondrial cytochrome C1 oxidase gene.
abstract: Our studies show that electrochemical impedance spectroscopy (EIS) and scanning electrochemical microscopy (SECM) of films of ds-DNA on gold allow us to distinguish between mitochondrial DNA fragments of the cytochrome c(1) oxidase (mt-Cox1) of three related species of the subfamily 'Bovinae' (Bos taurus, Bison bison, and Bison bonasus). In EIS, a perfectly matched DNA gives rise to a considerably larger charge transfer resistance R(ct) compared to mismatched pairings. Differences in charge transfer resistance, ΔR(ct), before and after the addition of Zn(2+) ions provide an additional tool for identification. In addition, all ds-DNA films were studied by SECM and their kinetic parameters were determined. Perfectly matched ds-DNAs are readily distinguished from mismatched duplexes by their lower rate constants. Our system can be used multiple times by dehybridization and rehybridization of capture strands up to the 250 pmole level.
authors.affiliation: Department of Chemistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: Mohtashim Hassan
authors.initials: MH
authors.lastname: Shamsi
authors.forename: Heinz-Bernhard
authors.initials: HB
authors.lastname: Kraatz
mesh_terms: D000818:Animals; D020137:Base Pair Mismatch / Q000235:genetics*; D001483:Base Sequence; D016164:Bison / Q000235:genetics; D002417:Cattle / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D058266:Dielectric Spectroscopy / Q000379:methods*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005607:Fraud; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D009841:Oligonucleotides; D003576:Electron Transport Complex IV