title: [Nitrates and nitrites in meat products--nitrosamines precursors].
abstract: OBJECTIVE
  To determine the content in nitrates and nitrites and the formation of two nitrosamines (N-nitrosodimethylamine--NDMA, and N-nitrosodiethylaamine--NDEA) in samples of chicken ham, dry Banat salami, dry French salami, traditional Romania sausages, and pork pastrami.
  
  
  METHODS
  Nitrites were determined by spectrophotometry with Peter-Griess reagent, and nitrates by the same method after reduction to nitrites with cadmium powder. High performance liquid chromatography with UV detection was used to determine nitrosamines.
  
  
  RESULTS
  The initial concentration of nitrates, nitrites, NDMA and NDEA in the samples ranged as follows: 14.10-60.40 mg NO3/kg, 2.70-26.70 mg NO2/kg, from non-detectable to 0.90 microg NDMA/kg, and from non-detectable to 0.27 microg NDEA/kg, respectively. After 28 days the concentrations were: 3.24-17.1 mg NO3/kg, 0.04 -1.87 mg NO2/kg, 0.8-29 microg NDMA/kg, and 11.6-61.9 microg NDEA/kg, respectively.
  
  
  CONCLUSIONS
  The decreased nitrate and nitrite and increased NDMA and NDEA concentrations prove that in food products nitrosamines are formed due to residual nitrite during their preservation. The determination of nitrasamines revealed levels much above the admitted maximal concentration for these food products.
authors.affiliation: Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi, Facultatea de Farmacie, Disciplina Chimia mediului şi alimentului.
authors.forename: Liliana
authors.initials: L
authors.lastname: Avasilcăi
authors.forename: Rodica
authors.initials: R
authors.lastname: Cuciureanu
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008461:Meat Products; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
chemical_list: D009566:Nitrates; D009573:Nitrites; D009602:Nitrosamines