title: Simultaneous determination of arsenite and arsenate in arsenic trioxide injection by dual detection ion chromatography.
abstract: This paper describes a rapid method to determine arsenite assay and arsenate impurity in Arsenic Trioxide Injection using a single conductivity detector. The arsenite assay was determined in a non-suppressed conductivity detection mode and arsenate impurity was quantified in a suppressed conductivity detection mode. Dual-conductivity detections were enabled by valve switching and time programming. The method was validated with respect to specificity, linearity, precision, accuracy, stability, and limit of quantification. The limit of detection and quantification for arsenite were 0.855 mg/L and 2.593 mg/L, and 0.044 mg/L and 0.133 mg/L for arsenate, respectively.
authors.affiliation: Metrohm India Limited, 13, First Avenue, Indira Nagar, Adyar, Chennai 600020, Tamilnadu, India. harisenthil@hotmail.com
authors.forename: N Harihara
authors.initials: NH
authors.lastname: Subramanian
authors.forename: M R
authors.initials: MR
authors.lastname: Hariganesh
authors.forename: R Ganesh
authors.initials: RG
authors.lastname: Jeevan
authors.forename: Ganga
authors.initials: G
authors.lastname: Radhakrishnan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry; D001149:Arsenates / Q000032:analysis*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000737:chemistry*; D018053:Arsenites / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004340:Drug Contamination; D004355:Drug Stability; D004553:Electric Conductivity; D004867:Equipment Design; D010087:Oxides / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents; D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D010087:Oxides; C015001:arsenite; C025657:arsenic acid; D000077237:Arsenic Trioxide