title: Methodology: simplified preparation of a DNA ladder using PCR.
abstract: Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mixing. Fragments of 100- to 1000-bp DNA were successfully amplified; the sequences showed 100% identity with lambda DNA. This prepared DNA marker displayed clear bands, indicating that it can be used for molecular studies.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Henan, P.R. China. wtianyuncn@126.com
authors.forename: T-Y
authors.initials: TY
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D004247:DNA