title: [Effect of calixarene C-107 on kinetic parameters of Na+, K(+)-ATPase in the plasma membrane of the uterus myocytes].
abstract: The inhibitory action of calixarene C-107 (5,17-diamino(2-pyridyl)methylphosphono- 11,23-di-tret-butyl-26,28-dihydroxy-25,27-dipropoxy-calix[4]arene) on Na+, K(+)-ATPase activity kinetic properties of myometrium perforated plasma membrane was investigated. It has been shown that the calixarene C-107 inhibiting Na+, K(+)-ATPase does not change the kinetic parameters (Km, nH) of reaction velocity dependence on substrate concentration. The constant Ka of enzyme activation by MgCl2 has complex dependence on calixarene C-107 concentration: it increases twice with growth of calixarene concentration up to 50 nM and decreases to the control level with further growth of calixarene concentration. The Hill cooperativity coefficient nH of activation by MgCl2 does not vary in the presence of calixarene C-107. Both ATP and MgCl2 have no influence on Na+, K(+)-ATPase constant of inhibition by calixarene C-107, but an increase of concentration of the mentioned physiological compounds causes the growth of cooperativity coefficient nH of enzymatic reaction inhibition by calixaren C-107.
authors.forename: T O
authors.initials: TO
authors.lastname: Veklich
authors.forename: O A
authors.initials: OA
authors.lastname: Shkrabak
authors.forename: R V
authors.initials: RV
authors.lastname: Rodik
authors.forename: V I
authors.initials: VI
authors.lastname: Kal'chenko
authors.forename: S O
authors.initials: SO
authors.lastname: Kosterin
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D047250:Calixarenes / Q000737:chemistry / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D015636:Magnesium Chloride / Q000494:pharmacology; D015394:Molecular Structure; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000201:enzymology; D009215:Myometrium / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; C533334:calixarene C-107; D015636:Magnesium Chloride; D047250:Calixarenes; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase