title: Monitoring the gestation period of rescued Formosan pangolin (Manis pentadactyla pentadactyla) with progesterone radioimmunoassay.
abstract: Eight species of pangolin have been identified in the world. However, understanding of pangolin reproductive biology has been limited to fragmentary records. In this study, the concentration of serum progesterone in three pregnant and two nonpregnant rescued female Formosan pangolins (Manis pentadactyla pentadactyla) was monitored using a commercial progesterone radioimmunoassay kit. During gestation, the serum progesterone of pregnant pangolins A, B, and C remained at 28.5-55 ng/ml (n = 31 samples), 10.9-50.1 ng/ml (n = 34), and 12.4 and 33.5 ng/ml with a peak at 47.6 ng/ml (n = 19), respectively, whereas the serum progesterone of nonpregnant pangolins D and E remained at 1.99 ± 1.62 ng/ml (n = 80) and 2.27 ± 1.64 ng/ml (n = 27), respectively. From this study, it was found that female pangolin weighing as low as 2.14 kg was already capable of reproduction. For pregnant pangolins to give birth to viable offspring, their body weight must increase significantly, 63.89 and 134.0% in the study, from the time of inception or early pregnancy until parturition. In addition, study has found that both viable offspring were born fully developed and exceeded 80 g in weight. The period of gestation was found to be as short as 318 or longer than 372 days. Therefore, the Formosan pangolin should only be able to reproduce once a year. This study is the first insight into hormone assay for determining the gestation period of pangolin. Further investigations on the same subject are necessary to establish criteria for the recognition of reproductive status in pangolins.
authors.affiliation: Taipei Zoo, Taipei, Taiwan.
authors.forename: Shih-Chien
authors.initials: SC
authors.lastname: Chin
authors.forename: Chen-Yen
authors.initials: CY
authors.lastname: Lien
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Chan
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Chen
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Yang
authors.forename: Lih-Seng
authors.initials: LS
authors.lastname: Yeh
mesh_terms: D000818:Animals; D000836:Animals, Zoo; D001835:Body Weight / Q000502:physiology; D004032:Diet; D005260:Female; D008322:Mammals / Q000502:physiology*; D008991:Monitoring, Physiologic / Q000379:methods*; D011247:Pregnancy / Q000502:physiology*; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay / Q000662:veterinary*; D013624:Taiwan; D013997:Time Factors
chemical_list: D011374:Progesterone