title: Effects of neuroactive steroids on cochlear hair cell death induced by gentamicin.
abstract: As neuroactive steroids, sex steroid hormones have non-reproductive effects. We previously reported that 17β-estradiol (βE2) had protective effects against gentamicin (GM) ototoxicity in the cochlea. In the present study, we examined whether the protective action of βE2 on GM ototoxicity is mediated by the estrogen receptor (ER) and whether other estrogens (17α-estradiol (αE2), estrone (E1), and estriol (E3)) and other neuroactive steroids, dehydroepiandrosterone (DHEA) and progesterone (P), have similar protective effects. The basal turn of the organ of Corti was dissected from Sprague-Dawley rats and cultured in a medium containing 100 μM GM for 48h. The effects of βE2 and ICI 182,780, a selective ER antagonist, were examined. In addition, the effects of other estrogens, DHEA and P were tested using this culture system. Loss of outer hair cells induced by GM exposure was compared among groups. βE2 exhibited a protective effect against GM ototoxicity, but its protective effect was antagonized by ICI 182,780. αE2, E1, and E3 also protected hair cells against gentamicin ototoxicity. DHEA showed a protective effect; however, the addition of ICI 182,780 did not affect hair cell loss. P did not have any effect on GM-induced outer hair cell death. The present findings suggest that estrogens and DHEA are protective agents against GM ototoxicity. The results of the ER antagonist study also suggest that the protective action of βE2 is mediated via ER but that of DHEA is not related to its conversion to estrogen and binding to ER. Further studies on neuroactive steroids may lead to new insights regarding cochlear protection.
authors.affiliation: Department of Otolaryngology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Nakamagoe
authors.forename: Keiji
authors.initials: K
authors.lastname: Tabuchi
authors.forename: Bungo
authors.initials: B
authors.lastname: Nishimura
authors.forename: Akira
authors.initials: A
authors.lastname: Hara
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D016923:Cell Death / Q000187:drug effects*; D003051:Cochlea / Q000166:cytology*; D019610:Cytoprotection / Q000187:drug effects*; D003687:Dehydroepiandrosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005839:Gentamicins / Q000009:adverse effects*; D006198:Hair Cells, Auditory / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004967:Estrogens; D005839:Gentamicins; D011960:Receptors, Estrogen; D013256:Steroids; D003687:Dehydroepiandrosterone; D011374:Progesterone; D004958:Estradiol