title: Red wine consumption is inversely associated with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-DNA adduct levels in prostate.
abstract: In humans, genetic variation and dietary factors may alter the biological effects of exposure to 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), one of the major heterocyclic amines generated from cooking meats at high temperatures that has carcinogenic potential through the formation of DNA adducts. Previously, we reported grilled red meat consumption associated with PhIP-DNA adduct levels in human prostate. In this study, we expanded our investigation to estimate the associations between beverage consumption and PhIP-DNA adduct levels in prostate for 391 prostate cancer cases. Of the 15 beverages analyzed, red wine consumption had the strongest association with PhIP-DNA adduct levels showing an inverse correlation in both tumor (P = 0.006) and nontumor (P = 0.002) prostate cells. Red wine consumption was significantly lower in African American compared with white cases, but PhIP-DNA adduct levels in prostate did not vary by race. In African Americans compared with whites, however, associations between red wine consumption and PhIP-DNA adduct levels were not as strong as associations with specific (e.g., SULT1A1 and UGT1A10 genotypes) and nonspecific (e.g., African ancestry) genetic variation. In a multivariable model, the covariate for red wine consumption explained a comparable percentage (13%-16%) of the variation in PhIP-DNA adduct levels in prostate across the two racial groups, but the aforementioned genetic factors explained 33% of the PhIP-DNA adduct variation in African American cases, whereas only 19% of the PhIP-DNA adduct variation in whites. We conclude that red wine consumption may counteract biological effects of PhIP exposure in human prostate, but genetic factors may play an even larger role, particularly in African Americans.
authors.affiliation: Department of Public Health Sciences, Henry Ford Health System, Detroit, MI 48202, USA. brybicki1@hfhs.org
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Rybicki
authors.forename: Christine
authors.initials: C
authors.lastname: Neslund-Dudas
authors.forename: Cathryn H
authors.initials: CH
authors.lastname: Bock
authors.forename: Nora L
authors.initials: NL
authors.lastname: Nock
authors.forename: Andrew
authors.initials: A
authors.lastname: Rundle
authors.forename: Michelle
authors.initials: M
authors.lastname: Jankowski
authors.forename: Albert M
authors.initials: AM
authors.lastname: Levin
authors.forename: Jennifer
authors.initials: J
authors.lastname: Beebe-Dimmer
authors.forename: Adnan T
authors.initials: AT
authors.lastname: Savera
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shirai
authors.forename: Deliang
authors.initials: D
authors.lastname: Tang
mesh_terms: D001741:Black or African American / Q000706:statistics & numerical data; D015239:Arylsulfotransferase / Q000235:genetics; D018736:DNA Adducts / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000235:genetics; D005838:Genotype; D014453:Glucuronosyltransferase / Q000235:genetics; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D008460:Meat / Q000009:adverse effects; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000208:ethnology / Q000209:etiology* / Q000378:metabolism*; D012307:Risk Factors; D044465:White People / Q000706:statistics & numerical data; D014920:Wine / Q000009:adverse effects*
chemical_list: C502239:2-amino-1-methyl-6-phenolimidazo(4,5-b)pyridine-DNA adduct; D018736:DNA Adducts; D004273:DNA, Neoplasm; D007093:Imidazoles; C476725:bilirubin uridine-diphosphoglucuronosyl transferase 1A10; D014453:Glucuronosyltransferase; D015239:Arylsulfotransferase; C117544:SULT1A1 protein, human