title: High dose granulocyte colony-stimulating factor enhances survival and hematopoietic reconstruction in canines irradiated by 2.3 Gy mixed fission neutron and gamma ray.
abstract: This study was purposed to evaluate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on hematopoietic reconstruction and survival in beagles exposed to mixed fission neutron and γ-ray. 13 beagles were unilaterally exposed to single dose of 2.3 Gy 90% neutrons. The experiments were divided into 3 groups: irradiation control group (no any treatment, n = 4), supportive care group (n = 5) and rhG-CSF plus supportive care group (n = 4, abbreviated as rhG-CSF group) in which the beagles were subcutaneously injected with 200 µg/kg of rhG-CSF early at half an hour and 24 hours post-irradiation respectively. The results showed that 2.3 Gy 90% neutron irradiation induced a severe acute radiation sickness of bone marrow type. The administration of rhG-CSF increased the survival rate from 60% in supportive care group to 100%. Twice injection of rhG-CSF in the first 24 hours reduced duration of neutropenia, enhanced neutrophil nadir and promoted neutrophil recovery when compared with control cohort administered clinical support. The number of colony-forming cells (CFU-GM, CFU-E, and BFU-E) in peripheral blood of rhG-CSF treated canines increased 2-to 5-fold relative to those of the supportive care group on day 3. All canines treated with rhG-CSF achieved hematopoietic reconstruction as evidenced by the pathological section of sternum while severe shortage of hemopoietic cells remained in the cohorts given supportive care alone. It is concluded that the combination of supportive care and high-dose rhG-CSF can accelerate hematopoietic recovery and enhance survival of dogs exposed to 2.3 Gy mixed neutron and gamma ray.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Zu-Yin
authors.initials: ZY
authors.lastname: Yu
authors.forename: Shuang
authors.initials: S
authors.lastname: Xing
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Ou
authors.forename: Guo-Lin
authors.initials: GL
authors.lastname: Xiong
authors.forename: Ling
authors.initials: L
authors.lastname: Xie
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhao
authors.forename: A-Ru-Na
authors.initials: AR
authors.lastname: Han
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Shan
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Zhen-Hu
authors.initials: ZH
authors.lastname: Zhao
authors.forename: Xin-Ru
authors.initials: XR
authors.lastname: Wang
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Cong
authors.forename: Qing-Liang
authors.initials: QL
authors.lastname: Luo
mesh_terms: D000818:Animals; D004285:Dogs; D005720:Gamma Rays / Q000009:adverse effects; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D006413:Hematopoietic System / Q000187:drug effects* / Q000528:radiation effects*; D033363:Neutron Diffraction; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D015996:Survival Rate
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor