title: Study of canine parvovirus evolution: comparative analysis of full-length VP2 gene sequences from Argentina and international field strains.
abstract: The continuous emergence of new strains of canine parvovirus (CPV), poorly protected by current vaccination, is a concern among breeders, veterinarians, and dog owners around the world. Therefore, the understanding of the genetic variation in emerging CPV strains is crucial for the design of disease control strategies, including vaccines. In this paper, we obtained the sequences of the full-length gene encoding for the main capsid protein (VP2) of 11 canine parvovirus type 2 (CPV-2) Argentine representative field strains, selected from a total of 75 positive samples studied in our laboratory in the last 9 years. A comparative sequence analysis was performed on 9 CPV-2c, one CPV-2a, and one CPV-2b Argentine strains with respect to international strains reported in the GenBank database. In agreement with previous reports, a high degree of identity was found among CPV-2c Argentine strains (99.6-100% and 99.7-100% at nucleotide and amino acid levels, respectively). However, the appearance of a new substitution in the 440 position (T440A) in four CPV-2c Argentine strains obtained after the year 2009 gives support to the variability observed for this position located within the VP2, three-fold spike. This is the first report on the genetic characterization of the full-length VP2 gene of emerging CPV strains in South America and shows that all the Argentine CPV-2c isolates cluster together with European and North American CPV-2c strains.
authors.affiliation: Centro de Virología Animal, Instituto de Ciencia y Tecnología Dr. Cesar Milstein CONICET, Saladillo 2468, C1440FFX Buenos Aires, Argentina.
authors.forename: Marina
authors.initials: M
authors.lastname: Gallo Calderón
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Wilda
authors.forename: Lorena
authors.initials: L
authors.lastname: Boado
authors.forename: Leticia
authors.initials: L
authors.lastname: Keller
authors.forename: Viviana
authors.initials: V
authors.lastname: Malirat
authors.forename: Marcela
authors.initials: M
authors.lastname: Iglesias
authors.forename: Nora
authors.initials: N
authors.lastname: Mattion
authors.forename: Jose
authors.initials: J
authors.lastname: La Torre
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina; D001208:Asia; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics*; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005060:Europe; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D008297:Male; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D016415:Sequence Alignment; D013020:South America
chemical_list: D036022:Capsid Proteins