title: Insulin-binding aptamer-conjugated graphene oxide for insulin detection.
abstract: This paper describes a simple and sensitive aptamer/graphene oxide (GO) based assay for insulin detection. GO can protect DNA from nuclease cleavage, but aptamers can be detached from the GO surface by specific target binding. This exposes the aptamers to enzymatic cleavage and releases the target for a new cycle. Cycling of targets leads to significant signal amplification and low LOD.
authors.affiliation: Xiangya Hospital, Central South University, P.O. Box 190. Changsha, Hunan 410008, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhu
authors.forename: Da
authors.initials: D
authors.lastname: Han
authors.forename: Huixia
authors.initials: H
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Liao
authors.forename: Kejing
authors.initials: K
authors.lastname: Zhang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D007328:Insulin / Q000032:analysis*; D010087:Oxides / Q000737:chemistry*; D052156:SELEX Aptamer Technique; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D007328:Insulin; D010087:Oxides; D006108:Graphite; D003850:Deoxyribonuclease I