title: Structure-function analysis of the beta regulatory subunit of protein kinase CK2 by targeting embryonic stem cell.
abstract: Programs that govern stem cell maintenance and pluripotency are dependent on extracellular factors and of intrinsic cell modulators. Embryonic stem (ES) cells with a specific depletion of the gene encoding the regulatory subunit of protein kinase CK2 (CK2β) revealed a viability defect. However, analysis of CK2β functions along the neural lineage established CK2β as a positive regulator for neural stem/progenitor cell (NSC) proliferation and multipotency. By using an in vitro genetic conditional approach, we demonstrate in this work that specific domains of CK2β involved in the regulatory function towards CK2 catalytic subunits are crucial structural determinants for ES cell homeostasis.
authors.affiliation: INSERM, U873, CEA, iRTSV/LTS, 17, Av. des Martyrs, 38054 Grenoble, France.
authors.forename: Léa
authors.initials: L
authors.lastname: Ziercher
authors.forename: Odile
authors.initials: O
authors.lastname: Filhol
authors.forename: Béatrice
authors.initials: B
authors.lastname: Laudet
authors.forename: Renaud
authors.initials: R
authors.lastname: Prudent
authors.forename: Claude
authors.initials: C
authors.lastname: Cochet
authors.forename: Thierry
authors.initials: T
authors.lastname: Buchou
mesh_terms: D000818:Animals; D047390:Casein Kinase II / Q000737:chemistry* / Q000378:metabolism*; D020134:Catalytic Domain; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002470:Cell Survival; D053595:Embryonic Stem Cells / Q000166:cytology / Q000201:enzymology*; D051379:Mice; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D058953:Neural Stem Cells / Q000166:cytology / Q000201:enzymology; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D013329:Structure-Activity Relationship; D013724:Teratoma / Q000473:pathology
chemical_list: D050505:Mutant Proteins; D047390:Casein Kinase II