title: Perfluorochemical liquid-adenovirus suspensions enhance gene delivery to the distal lung.
abstract: WE COMPARED LUNG DELIVERY METHODS OF RECOMBINANT ADENOVIRUS (RAD): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2-4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.
authors.affiliation: The Cardiopulmonary Research Institute and Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital, Mineola, NY 11507, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Kazzaz
authors.forename: Marlene S
authors.initials: MS
authors.lastname: Strayer
authors.forename: Jichuan
authors.initials: J
authors.lastname: Wu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Malone
authors.forename: Hshi-Chi
authors.initials: HC
authors.lastname: Koo
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Shaffer
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Davis
authors.forename: David S
authors.initials: DS
authors.lastname: Strayer
authors.forename: Marla R
authors.initials: MR
authors.lastname: Wolfson