title: Effect of surfactants on PAH biodegradation by a bacterial consortium and on the dynamics of the bacterial community during the process.
abstract: The aim of this work was to evaluate the effect of a non-biodegradable (Tergitol NP-10) and a biodegradable (Tween-80) surfactant on growth, degradation rate and microbial dynamics of a polycyclic aromatic hydrocarbon (PAHs) degrading consortium (C2PL05) from a petroleum polluted soil, applying cultivable and non cultivable techniques. Growth and degradation rate were significantly lower with Tergitol NP-10 than that with Tween-80. Toxicity did not show any significant reduction with Tergitol NP-10 whereas with Tween-80 toxicity was almost depleted (30%) after 40 days. Regarding to the cultured bacteria, Pseudomonas and Stenotrophomonas groups were dominant during PAH degradation with Tergitol NP-10, whereas Enterobacter and Stenotrophomonas were dominant with Tween-80. DGGE analyses (PRIMER and MDS) showed that bacteria composition was more similar between treatments when PAHs were consumed than when PAHs concentration was still high. Community changes between treatments were a consequence of Pseudomonas sp., Sphingomonas sp., Sphingobium sp. and Agromonas sp.
authors.affiliation: Department of Biology and Geology, ESCET, Universidad Rey Juan Carlos, C/ Tulipán s/n, E-28933 Móstoles, Madrid, Spain.
authors.forename: N
authors.initials: N
authors.lastname: González
authors.forename: R
authors.initials: R
authors.lastname: Simarro
authors.forename: M C
authors.initials: MC
authors.lastname: Molina
authors.forename: L F
authors.initials: LF
authors.lastname: Bautista
authors.forename: L
authors.initials: L
authors.lastname: Delgado
authors.forename: J A
authors.initials: JA
authors.lastname: Villa
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D011083:Polycyclic Compounds / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D011083:Polycyclic Compounds; D013501:Surface-Active Agents