title: The large Rep protein of adeno-associated virus type 2 is polyubiquitinated.
abstract: Five adenovirus (Ad) gene products are required for efficient replication of co-infecting adeno-associated virus (AAV); however, the combined net enhancement by these factors is composed of both positive and negative effects. Similar to previous results with AAV Rep52, AAV2 large Rep was targeted for ubiquitination and degradation by the Ad E4orf6/E1b 55 kDa, cullin 5-containing, E3-ubiquitin ligase. Additionally, large Rep was targeted for ubiquitination via extension of ubiquitin lysine K48 and K63 both in the presence and absence of E4orf6.
authors.affiliation: Department of Molecular Microbiology and Immunology, Bond Life Sciences Center, University of Missouri-Columbia, Columbia, USA.
authors.forename: Loretta
authors.initials: L
authors.lastname: Sukhu
authors.affiliation: Department of Molecular Microbiology and Immunology, Bond Life Sciences Center, University of Missouri-Columbia, Columbia, USA.
authors.forename: David
authors.initials: D
authors.lastname: Pintel
mesh_terms: D002460:Cell Line; D004268:DNA-Binding Proteins / Q000737:chemistry*; D000229:Dependovirus / Q000235:genetics* / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006801:Humans; D025821:Polyubiquitin / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D054875:Ubiquitination; D014764:Viral Proteins / Q000737:chemistry*; D014779:Virus Replication
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins; D025821:Polyubiquitin; C068734:rep proteins, Adeno-associated virus 2; D044767:Ubiquitin-Protein Ligases