title: Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5).
abstract: OBJECTIVE
  The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells.
  
  
  METHODS
  Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test.
  
  
  RESULTS
  A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods (P < .001). The cytotoxic effect of both materials was concentration dependant.
  
  
  CONCLUSIONS
  Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.
authors.affiliation: Faculty of Oral and Dental Medicine, Misr International University, Cairo, Egypt. daliafayyad222@yahoo.com
authors.forename: Dalia
authors.initials: D
authors.lastname: Mukhtar-Fayyad
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry; D004396:Coloring Agents; D003470:Culture Media; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006813:Humidity; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology; D053761:Nanocomposites / Q000737:chemistry; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors
chemical_list: C557009:BioAggregate; D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D004396:Coloring Agents; D003470:Culture Media; D006882:Hydroxyapatites; D012387:Root Canal Filling Materials; D017640:Silicates; D013778:Tetrazolium Salts; D013844:Thiazoles; D017886:Durapatite; C020243:calcium phosphate; C022616:thiazolyl blue; D002126:Calcium Hydroxide; C031293:calcium silicate