title: Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats.
abstract: A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats.
authors.affiliation: Enfer Scientific, Unit T, M7 Business Park, Newhall, Naas, Co. Kildare, Ireland.
authors.forename: Eduard
authors.initials: E
authors.lastname: Shuralev
authors.forename: Padraig
authors.initials: P
authors.lastname: Quinn
authors.forename: Mairead
authors.initials: M
authors.lastname: Doyle
authors.forename: Anthony
authors.initials: A
authors.lastname: Duignan
authors.forename: Hang Fai
authors.initials: HF
authors.lastname: Kwok
authors.forename: Javier
authors.initials: J
authors.lastname: Bezos
authors.forename: Shane A
authors.initials: SA
authors.lastname: Olwill
authors.forename: Eamonn
authors.initials: E
authors.lastname: Gormley
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
authors.forename: Margaret
authors.initials: M
authors.lastname: Good
authors.forename: William C
authors.initials: WC
authors.lastname: Davis
authors.forename: John
authors.initials: J
authors.lastname: Clarke
authors.forename: Clare
authors.initials: C
authors.lastname: Whelan
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006041:Goats / Q000097:blood / Q000821:virology*; D049449:Luminescence; D009163:Mycobacterium bovis; D012680:Sensitivity and Specificity; D013030:Spain; D014374:Tuberculin Test / Q000662:veterinary; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*