title: Intrastrand locks increase duplex stability and base pairing selectivity.
abstract: Oligodeoxynucleotide probes with disulfide locks between neighboring nucleobases show increases in melting point for duplexes with RNA target strands of up to 7.6 °C. The weakly pairing TT dimers are replaced with locked 2'-deoxy-5-(thioalkynyl)uridine residues via automated synthesis.
authors.affiliation: Institute for Organic Chemistry, University of Stuttgart, Pfaffenwaldring 55, 70565 Stuttgart, Germany.
authors.forename: Cora
authors.initials: C
authors.lastname: Prestinari
authors.forename: Clemens
authors.initials: C
authors.lastname: Richert
mesh_terms: D020137:Base Pair Mismatch; D020029:Base Pairing; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D044366:Transition Temperature; D014466:Ultraviolet Rays
chemical_list: D004220:Disulfides; D009838:Oligodeoxyribonucleotides; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C477371:locked nucleic acid; D012313:RNA