title: TLR4 signal transduction pathways neutralize the effect of Fas signals on glioblastoma cell proliferation and migration.
abstract: The Fas pathway is described as an activator of the glioblastoma proliferation by increasing the pathogenicity of this tumour. The lipopolysaccharide (LPS) pathway depending on Toll-like receptor 4 (TLR4) could limit the glioblastoma spreading. Here, Fas and TLR4 pathways were activated in glioblastoma cell lines by an agonist antibody and/or LPS treatment. Activation of the Fas pathway or of the TLR4 pathway induced cell proliferation. However, simultaneous treatment with agonist antibody and LPS decreased proliferation. This anti-proliferative effect was caspase dependent, and a decreased cell migration and matrix metalloproteinase (MMP)-9 expression were also observed. Both TLR4 and MMP-9 were highly expressed in human glioblastoma tissues. These data suggest that TLR4 signal transduction pathways neutralize proliferation and migration induced by Fas pathway activation in glioblastoma cell lines.
authors.affiliation: EA 3842 (Homéostasie Cellulaire et Pathologies), Institut Fédératif de Recherche 145, Facultés de Médecine et de Pharmacie, Université de Limoges, Limoges, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Sarrazy
authors.forename: Nicolas
authors.initials: N
authors.lastname: Vedrenne
authors.forename: Fabrice
authors.initials: F
authors.lastname: Billet
authors.forename: Nelly
authors.initials: N
authors.lastname: Bordeau
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lepreux
authors.forename: Anne
authors.initials: A
authors.lastname: Vital
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Jauberteau
authors.forename: Alexis
authors.initials: A
authors.lastname: Desmoulière
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism*
chemical_list: C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D019014:fas Receptor; D020780:Matrix Metalloproteinase 9