title: On-line coupling of automatic solid-phase extraction and HPLC for determination of carotenoids in serum.
abstract: The automated method developed for the determination of carotenoids uses 200 μL of serum, which was mixed with 400 μL of tetrahydrofuran, vortexed for 1 min, settled for 10 min, centrifuged for 6 min and the supernatant injected into an automatic solid-phase extraction (SPE) system for cleanup-preconcentration. A 10% water-acetonitrile mobile phase at 1.5 mL min(-1) eluted the retained compounds and transferred them on-line to a reversed-phase analytical column for individual separation of the target analytes. Visible detection was performed at 450 and 460 nm. The detection limits for the target analytes were between 3 and 30 ng mL(-1); the precision (expressed as relative standard deviation) ranged between 2.83 and 5.06% for repeatability and between 3.80 and 7.40% for within laboratory reproducibility. The total analysis time was 18 min. The proposed method is reliable, robust, and has an excellent potential for high-throughput use in both clinical and research laboratories.
authors.affiliation: Department of Analytical Chemistry, Annex C-3, Campus of Rabanales, University of Córdoba, E-14071, Córdoba, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Ferreiro-Vera
authors.forename: J M
authors.initials: JM
authors.lastname: Mata-Granados
authors.forename: J M
authors.initials: JM
authors.lastname: Quesada Gómez
authors.forename: M D
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001331:Automation; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002338:Carotenoids / Q000097:blood* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009862:Online Systems; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D055815:Young Adult
chemical_list: D012997:Solvents; D002338:Carotenoids