title: Standardized flavonoid-rich Eugenia jambolana seed extract retards in vitro and in vivo LDL oxidation and expression of VCAM-1 and P-selectin in atherogenic rats.
abstract: The present inventory evaluates anti-atherogenic potential of flavonoid-rich Eugenia jambolana seed extract (EJSE) against in vitro low-density lipoprotein (LDL) oxidation, foam cell formation, and atherogenic (ATH) diet-induced experimental atherosclerosis in rats. EJSE was able to prevent in vitro LDL oxidation and oxidized LDL-induced macrophage foam cell formation. Also, EJSE supplementation to ATH rats significantly minimized increment in serum markers of LDL oxidation. The ex vivo oxidation indices were also minimized in LDL of EJSE-treated animals. Microscopic evaluation of thoracic aorta of ATH + EJSE rats recorded minimal evidence of atheromatous plaque formation, accumulation of lipid laden macrophages, calcium deposition, and expression of cell adhesion molecules (vascular cell adhesion molecule-1 and P-selectin). This is the first scientific report that demonstrates anti-atherogenic potential of EJSE and warrants further evaluation at clinical level.
authors.affiliation: Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, The M. S. University of Baroda, Vadodara, Gujarat.
authors.forename: Ravirajsinh N
authors.initials: RN
authors.lastname: Jadeja
authors.forename: Menaka C
authors.initials: MC
authors.lastname: Thouaojam
authors.forename: Jayantha M
authors.initials: JM
authors.lastname: Sankhari
authors.forename: Mahendra
authors.initials: M
authors.lastname: Jain
authors.forename: Ranjitsinh V
authors.initials: RV
authors.lastname: Devkar
authors.forename: A V
authors.initials: AV
authors.lastname: Ramachandran
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood* / Q000517:prevention & control; D002460:Cell Line; D004036:Diet, Atherogenic / Q000379:methods; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005786:Gene Expression Regulation; D006801:Humans; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors / Q000097:blood*; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction / Q000187:drug effects; D019007:P-Selectin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D012639:Seeds / Q000737:chemistry; D027842:Syzygium / Q000737:chemistry; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D005419:Flavonoids; D008077:Lipoproteins, LDL; D019007:P-Selectin; D010936:Plant Extracts; D019010:Vascular Cell Adhesion Molecule-1