title: Temporal variation of pharmaceuticals in an urban and agriculturally influenced stream.
abstract: Pharmaceuticals have become ubiquitous in the aquatic environment. Previous studies consistently demonstrate the prevalence of pharmaceuticals in freshwater but we do not yet know how concentrations vary over time within a given system. Two sites in central Indiana with varying land use in the surrounding watershed (suburban and agricultural) were sampled monthly for pharmaceutical concentrations and stream physiochemical parameters. Sediment samples were also collected at each sampling event for measurement of δ(15)N natural abundance and sediment organic content. Across sites and sampling events, twelve pharmaceuticals were detected including acetaminophen, caffeine, carbamazepine, cotinine, N,N-diethyl-meta-toluamide (DEET), gemfibrozil, ibuprofen, sulfadimethoxine, sulfamethazine, sulfamethoxazole, triclosan, and trimethoprim. Sulfathiazole, lincomycin, and tylosin were not detected at either site at any time. The agriculturally-influenced site had comparable pharmaceutical concentrations to the urban-influenced site. In general, pharmaceutical concentrations increased during winter at both sites and decreased during spring and summer. Multiple regression analyses indicated that water column dissolved oxygen, the number of days since precipitation, and solar radiation influenced total pharmaceutical concentration in the urban-influenced site; whereas pH, chlorophyll a concentration, and total amount of rainfall in the previous 10 days influenced total pharmaceutical concentrations in the agriculturally-influenced site. Pharmaceutical concentrations were not correlated with sediment δ(15)N across or within sites. However, sediment in the urban-influenced site had higher mean δ(15)N signatures relative to sediment in the agriculturally-influenced site. These data indicate pharmaceuticals are persistent in aquatic ecosystems influenced by both agricultural and suburban activity. Pharmaceuticals are designed to have a physiological effect; therefore, it is likely that they may also influence aquatic organisms, potentially threatening freshwater ecosystem health.
authors.affiliation: Department of Biology, Ball State University, Muncie, Indiana 47306, USA. amveach@k-state.edu
authors.forename: Allison M
authors.initials: AM
authors.lastname: Veach
authors.forename: Melody J
authors.initials: MJ
authors.lastname: Bernot
mesh_terms: D000383:Agriculture; D002947:Cities; D004777:Environment; D004784:Environmental Monitoring / Q000379:methods*; D019015:Geologic Sediments / Q000737:chemistry*; D007196:Indiana; D009587:Nitrogen Isotopes / Q000032:analysis*; D004366:Nonprescription Drugs / Q000032:analysis*; D012044:Regression Analysis; D045483:Rivers / Q000737:chemistry*; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D009587:Nitrogen Isotopes; D004366:Nonprescription Drugs; D014874:Water Pollutants, Chemical