title: Molecular identification of three Trichinella isolates from Heilongjiang Province, People's Republic of China.
abstract: DNAs of Trichinella dog isolate (HC), Trichinella swine isolate (HH) and Trichinella cat isolate (SW), obtained from Heilongjiang Province, were amplified by the fragments of 18S rDNA and ITS2. Two reference strains, Trichinella spiralis (ISS3) and Trichinella nativa (ISS10) were used for sequence comparison. Sequence and dendrogram analysis indicated that HC belonged to T. nativa and HH together with SW belonged to T. spiralis. This method permits rapid species identification of Trichinella isolates, although further evaluation is required before precisely identification.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, PR China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Lu
authors.forename: Caixia
authors.initials: C
authors.lastname: Han
authors.forename: Jinyu
authors.initials: J
authors.lastname: Yang
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Li
authors.forename: Mingxin
authors.initials: M
authors.lastname: Song
mesh_terms: D000818:Animals; D002415:Cats; D002681:China; D018509:DNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry; D021903:DNA, Ribosomal Spacer / Q000737:chemistry; D004285:Dogs; D051379:Mice; D018482:Muscle, Skeletal / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014234:Trichinella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014235:Trichinellosis / Q000469:parasitology; D001503:Ursidae
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S