title: Live birth after intrauterine insemination with spermatozoa from an oligoasthenozoospermic patient vitrified without permeable cryoprotectants.
abstract: We report the first case of a healthy baby born after intrauterine  insemination with vitrified swim-up spermatozoa from an oligoasthenozoospermic  patient. A 39-year-old patient was subjected to intrauterine insemination with  spermatozoa from her 35-year-old husband, diagnosed with  oligoasthenozoospermia. The swim-up spermatozoa from 2 ejaculates were  suspended in a culture medium supplemented with 1% human serum albumin and  0.25 M sucrose. Three hermetically packaged 100-μL sperm portions (each  containing 1.0 × 10(6) spermatozoa/mL) were vitrified by direct  plunging into liquid nitrogen. The presence of leukocytes in the ejaculates  was determined by indirect immunofluorescence assay. Production of reactive  oxygen species was measured by chemiluminescent assay. Before intrauterine  insemination, all portions were warmed in culture medium at 37°C, and the  spermatozoa were concentrated by centrifugation; they were then resuspended in  500 μL of culture medium and inseminated. The cell suspension used for  insemination displayed 60% progressive motility. Clinical pregnancy was  confirmed at 7 weeks of gestation, and a healthy baby was born at term. Our  results show that a successful intrauterine insemination can be achieved with  aseptic vitrification of spermatozoa.
authors.affiliation: Department of Preclinical Sciences, La Frontera University, Temuco, Chile.
authors.forename: Raul
authors.initials: R
authors.lastname: Sanchez
authors.forename: Vladimir
authors.initials: V
authors.lastname: Isachenko
authors.forename: Anna M
authors.initials: AM
authors.lastname: Petrunkina
authors.forename: Jennie
authors.initials: J
authors.lastname: Risopatron
authors.forename: Mabel
authors.initials: M
authors.lastname: Schulz
authors.forename: Evgenia
authors.initials: E
authors.lastname: Isachenko
mesh_terms: D000328:Adult; D053627:Asthenozoospermia; D015925:Cryopreservation / Q000379:methods; D005260:Female; D006801:Humans; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D050498:Live Birth; D008297:Male; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D058989:Vitrification