title: Immbolization of uricase enzyme in Langmuir and Langmuir-Blodgett films of fatty acids: possible use as a uric acid sensor.
abstract: Preserving the enzyme structure in solid films is key for producing various bioelectronic devices, including biosensors, which has normally been performed with nanostructured films that allow for control of molecular architectures. In this paper, we investigate the adsorption of uricase onto Langmuir monolayers of stearic acid (SA), and their transfer to solid supports as Langmuir-Blodgett (LB) films. Structuring of the enzyme in β-sheets was preserved in the form of 1-layer LB film, which was corroborated with a higher catalytic activity than for other uricase-containing LB film architectures where the β-sheets structuring was not preserved. The optimized architecture was also used to detect uric acid within a range covering typical concentrations in the human blood. The approach presented here not only allows for an optimized catalytic activity toward uric acid but also permits one to explain why some film architectures exhibit a superior performance.
authors.affiliation: Instituto de Ciências Ambientais, Química e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil.
authors.forename: Nathaly C M
authors.initials: NC
authors.lastname: Zanon
authors.forename: Osvaldo N
authors.initials: ON
authors.lastname: Oliveira
authors.forename: Luciano
authors.initials: L
authors.lastname: Caseli
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D007103:Immobilization; D008567:Membranes, Artificial; D013229:Stearic Acids / Q000737:chemistry*; D013499:Surface Properties; D014503:Urate Oxidase / Q000737:chemistry* / Q000378:metabolism; D014527:Uric Acid / Q000032:analysis* / Q000378:metabolism
chemical_list: D008567:Membranes, Artificial; D013229:Stearic Acids; D014527:Uric Acid; C031183:stearic acid; D014503:Urate Oxidase