title: Supplementation of inorganic phosphate enhancing the removal efficiency of tannery sludge-borne Cr through bioleaching.
abstract: Four inorganic mineral nutrients including NH4+, K+, Mg2+ and soluble inorganic phosphate (Pi) were investigated to reveal the potential limiting nutrients for tannery sludge bioleaching process driven by Acidithiobacillus species, and the feasibility of supplementing the limiting nutrients to accelerate tannery sludge bioleaching was studied in the present study. It was found that the concentration of Pi was lower than 3.5 mg/L throughout the whole bioleaching process, which is the most probable restricting nutrient for tannery sludge bioleaching. Further experiments revealed that the deficiency of Pi could seriously influence the growth of Acidithiobacillus thiooxidans and lower its oxidization capacity for S0, and the limiting concentration of Pi for the growth of A. thiooxidans was 6 mg/L. The low concentration of soluble Pi in sludge matrix was resulted from the extremely strong sorbing/binding capacity of tannery sludge for phosphate. The supplementation of more than 1.6 g/L KH2PO4 into tannery sludge bioleaching system could effectively stimulate the growth of Acidithiobacillus species, enhance Cr removal rate and further shorten tannery sludge bioleaching period from 10 days to 7 days. Therefore, inorganic phosphate supplementation is an effective and feasible method to accelerate tannery sludge bioleaching process, and the optimum dosage of KH2PO4 was 1.6 g/L for tannery sludge with 5.1% of total solids.
authors.affiliation: Department of Environmental Engineering, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.
authors.forename: Guanyu
authors.initials: G
authors.lastname: Zheng
authors.forename: Lixiang
authors.initials: L
authors.lastname: Zhou
mesh_terms: D042763:Acidithiobacillus / Q000378:metabolism*; D002857:Chromium / Q000302:isolation & purification* / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism; D012722:Sewage; D013633:Tanning
chemical_list: D010710:Phosphates; D012722:Sewage; D002857:Chromium