title: Effect of andrographolide on cysteamine-induced duodenal ulcer in rats.
abstract: The aim of this study was to evaluate the gastroprotective efficacy of andrographolide isolated from Andrographis paniculata in rats induced with duodenal ulcers. Duodenal ulcers were induced by cysteamine administration in rats pretreated with 3 mg kg⁻¹ BW day⁻¹ of andrographolide for 30 days. Ulcer score, myeloperoxidase activity, TBARS level, GSH/GSSG ratio and enzyme antioxidants were measured in the duodenal tissue. Brush border and basolateral membranes were isolated to assay sucrase, maltase, alkaline phosphatase and total ATPases. Ulcer score was significantly minimised in rats pretreated with andrographolide. Elevation in myeloperoxidase and TBARS levels were found to be minimised significantly due to andrographolide treatment. Membrane-bound enzyme activities and the thiol redox status of glutathione were significantly maintained in duodenal mucosa of rats that received andrographolide. This study reveals that the major component of A. paniculata, andrographolide, has potent antiulcer properties that are most likely caused by minimising inflammatory changes, counteracting free radical formation and maintaining the thiol redox status in the duodenum.
authors.affiliation: Department of Biochemistry, Bharathi Women's College-Affliated to University of Madras, Chennai 600108, Tamil Nadu, India.
authors.forename: Saranya
authors.initials: S
authors.lastname: Panneerselvam
authors.forename: Geetha
authors.initials: G
authors.lastname: Arumugam
authors.forename: Narmadha Selvamathy Selvaperumal Munis
authors.initials: NS
authors.lastname: Karthikeyan
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D003543:Cysteamine / Q000008:administration & dosage / Q000009:adverse effects; D004195:Disease Models, Animal; D004224:Diterpenes / Q000008:administration & dosage / Q000627:therapeutic use; D004381:Duodenal Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology; D004386:Duodenum / Q000187:drug effects / Q000201:enzymology; D005978:Glutathione / Q000032:analysis; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D010936:Plant Extracts / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013393:Sucrase / Q000032:analysis; D000520:alpha-Glucosidases / Q000032:analysis
chemical_list: D000897:Anti-Ulcer Agents; D004224:Diterpenes; D010936:Plant Extracts; C030419:andrographolide; D003543:Cysteamine; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D013393:Sucrase; D005978:Glutathione