title: Distribution of TRPC6 in the cerebrospinal fluid-contacting nucleus of rat brain parenchyma and its expression in morphine dependence and withdrawal.
abstract: To investigate the role of cerebrospinal fluid contacting nucleus (CSF-CN) and the changes of TRPC6 expression in morphine dependence and withdrawal. Male Sprague-Dawley rats (250 ± 50 g) were randomly divided into four groups: the normal group (N); the saline group (S); the morphine dependence group (D); the morphine withdrawal group (W). All animals in each group were tested the morphine withdrawal-like behavioral signs by total withdrawal scores. Double-labeled immunofluorescent technique and laser scanning confocal microscopy were used to identify the expression of TRPC6 in CSF-CN. TRPC6 labeled neurons were found in CSF-CN and the number of CB-HRP/TRPC6 double labeled neurons in CSF-CN significantly increased. TRPC6 existed in CSF-CN of the normal rats and its expression in morphine dependence and withdrawal increased. CSF-CN might participate in the development of morphine dependence and withdrawal by the up-regulated expression of TRPC6.
authors.affiliation: Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical College, 84 Huaihai West Road, Xuzhou City, 221002 Jiangsu Province, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Wu
authors.forename: Zi-jun
authors.initials: ZJ
authors.lastname: Zhao
authors.forename: Chun
authors.initials: C
authors.lastname: Xu
authors.forename: Li-cai
authors.initials: LC
authors.lastname: Zhang
mesh_terms: D000818:Animals; D008297:Male; D023421:Models, Animal; D009020:Morphine / Q000009:adverse effects*; D009021:Morphine Dependence / Q000378:metabolism*; D009270:Naloxone / Q000494:pharmacology; D010487:Periaqueductal Gray / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*; D050052:TRPC Cation Channels / Q000378:metabolism*
chemical_list: D050052:TRPC Cation Channels; C492145:Trpc6 protein, rat; D009270:Naloxone; D009020:Morphine