title: Stable-isotope labeling with amino acids in nematodes.
abstract: We describe an approach for accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted stable-isotope labeling with amino acids in cell culture (SILAC) for nematodes by feeding worms a heavy lysine- and heavy arginine-labeled Escherichia coli strain and report a genetic solution to elminate the problem of arginine-to-proline conversion. Combining our approach with quantitative proteomics methods, we characterized the heat-shock response in worms.
authors.affiliation: Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Larance
authors.forename: Aymeric P
authors.initials: AP
authors.lastname: Bailly
authors.forename: Ehsan
authors.initials: E
authors.lastname: Pourkarimi
authors.forename: Ronald T
authors.initials: RT
authors.lastname: Hay
authors.forename: Grant
authors.initials: G
authors.lastname: Buchanan
authors.forename: Sarah
authors.initials: S
authors.lastname: Coulthurst
authors.forename: Dimitris P
authors.initials: DP
authors.lastname: Xirodimas
authors.forename: Anton
authors.initials: A
authors.lastname: Gartner
authors.forename: Angus I
authors.initials: AI
authors.lastname: Lamond
mesh_terms: D000818:Animals; D001120:Arginine / Q000737:chemistry* / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000378:metabolism*; D002478:Cells, Cultured; D004926:Escherichia coli / Q000737:chemistry; D018869:Heat-Shock Response / Q000502:physiology; D007553:Isotope Labeling / Q000379:methods*; D008239:Lysine / Q000737:chemistry*; D011392:Proline / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics
chemical_list: D001120:Arginine; D011392:Proline; D008239:Lysine