title: One-pot three-enzyme synthesis of UDP-GlcNAc derivatives.
abstract: A Pasteurella multocida N-acetylglucosamine 1-phosphate uridylyltransferase (PmGlmU) was cloned and used efficiently with an N-acetylhexosamine 1-kinase (NahK_ATCC55813) and an inorganic pyrophosphatase (PmPpA) for one-pot three-enzyme synthesis of UDP-GlcNAc derivatives with or without further chemical diversification.
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Vireak
authors.initials: V
authors.lastname: Thon
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Li
authors.forename: Hai
authors.initials: H
authors.lastname: Yu
authors.forename: Li
authors.initials: L
authors.lastname: Ding
authors.forename: Kam
authors.initials: K
authors.lastname: Lau
authors.forename: Jingyao
authors.initials: J
authors.lastname: Qu
authors.forename: Liana
authors.initials: L
authors.lastname: Hie
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D000117:Acetylglucosamine / Q000138:chemical synthesis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology; D043564:Inorganic Pyrophosphatase / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000378:metabolism*; D016979:Pasteurella multocida / Q000201:enzymology; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D014537:Uridine Diphosphate N-Acetylglucosamine; D009713:Nucleotidyltransferases; C023226:UDPacetylglucosamine pyrophosphorylase; D043564:Inorganic Pyrophosphatase; D000117:Acetylglucosamine