title: Down regulation of gene related sex hormone synthesis pathway in mouse testes by miroestrol and deoxymiroestrol.
abstract: Miroestrol and deoxymiroestrol are phytoestrogens isolated from tuberous root of Pueraria candollei var. mirifica. Modulatory effects of miroestrol and deoxymiroestrol on enzymes involved in sex-hormone synthesis pathway in male C57BL/6 mice were investigated using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Miroestrol and deoxymiroestrol suppressed the expressions of 3β-HSD, 17β-HSD1, and CYP17 while CYP19 mRNA expression was slightly decreased. In addition, the expression of 17β-HSD2 was induced in correlation with those did by estradiol. These observations supported that miroestrol and deoxymiroestrol could exhibit the same effect as estradiol regarding regulation of testicular gene related sex hormone synthesis pathway.
authors.affiliation: Academic Office for Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
authors.forename: Latiporn
authors.initials: L
authors.lastname: Udomsuk
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Juengwatanatrakul
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D012739:Gonadal Steroid Hormones / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D048789:Phytoestrogens / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D029908:Pueraria / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000302:isolation & purification / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism
chemical_list: D003374:Coumarins; D004791:Enzyme Inhibitors; D012739:Gonadal Steroid Hormones; D048789:Phytoestrogens; D010936:Plant Extracts; D012333:RNA, Messenger; D013256:Steroids; C406602:deoxymiroestrol; C571572:miroestrol; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase