title: Identification of risk loci for necrotizing meningoencephalitis in Pug dogs.
abstract: Due to their unique population structure, purebred dogs have emerged as a key model for the study of complex genetic disorders. To evaluate the utility of a newly available high-density canine whole-genome array with >170,000 single nucleotide polymorphisms (SNPs), genome-wide association was performed on a small number of case and control dogs to determine disease susceptibility loci in canine necrotizing meningoencephalitis (NME), a disorder with known non-Mendelian inheritance that shares clinical similarities with atypical variants of multiple sclerosis in humans. Genotyping of 30 NME-affected Pug dogs and 68 healthy control Pugs identified 2 loci associated with NME, including a region within dog leukocyte antigen class II on chromosome 12 that remained significant after Bonferroni correction. Our results support the utility of this high-density SNP array, confirm that dogs are a powerful model for mapping complex genetic disorders and provide important preliminary data to support in depth genetic analysis of NME in numerous affected breeds.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
authors.forename: Renee M
authors.initials: RM
authors.lastname: Barber
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Schatzberg
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Corneveaux
authors.forename: April N
authors.initials: AN
authors.lastname: Allen
authors.forename: Brian F
authors.initials: BF
authors.lastname: Porter
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Pruzin
authors.forename: Simon R
authors.initials: SR
authors.lastname: Platt
authors.forename: Marc
authors.initials: M
authors.lastname: Kent
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Huentelman
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D008590:Meningoencephalitis / Q000235:genetics / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors
chemical_list: D002352:Carrier Proteins; D015395:Histocompatibility Antigens Class I; C039692:histocompatibility antigen DLA