title: Wnt5a potentiates U46619-induced platelet aggregation via the PI3K/Akt pathway.
abstract: Platelet aggregation plays crucial roles in the formation of hemostatic plugs and thrombosis. Although it was recently shown that canonical Wnt signaling negatively regulates platelet aggregation, the role of non-canonical Wnt signaling remains unknown. Here, we observed that Wnt5a, one of the non-canonical Wnts, positively regulated platelet aggregation. Platelet aggregation was potentiated by the addition of Wnt5a to collagen-or U46619-induced rat platelet rich plasma (PRP). Treatment with Wnt5a to U46619-stimulated PRP resulted in an increase in the level of phosphorylated Akt, whereas phosphorylation of PKCδ and JNK1 was unaffected. In addition, inhibition of PI3K blocked the potentiating effect of Wnt5a. Taken together, these results suggest that Wnt5a potentiates U46619-induced platelet aggregation via the PI3K/Akt pathway.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Sewoon
authors.initials: S
authors.lastname: Kim
authors.forename: Hye Sook
authors.initials: HS
authors.lastname: Yun-Choi
authors.forename: Eek-Hoon
authors.initials: EH
authors.lastname: Jho
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / Q000494:pharmacology; D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets; D002478:Cells, Cultured; D051058:Oncogene Protein v-akt / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects; D053657:Platelet-Rich Plasma; D011518:Proto-Oncogene Proteins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D051153:Wnt Proteins / Q000494:pharmacology*; D000071818:Wnt-5a Protein; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D014662:Vasoconstrictor Agents; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D051058:Oncogene Protein v-akt; D000077191:Wortmannin