title: Identification of RHOXF2 (PEPP2) as a cancer-promoting gene by expression             cloning.
abstract: Multiple mutations contribute to establish cancers. We have searched for             potential oncogenes by screening cDNA libraries derived from gastric cancer cell             lines, pancreatic cancer cell lines and glioma cell lines, using retrovirus-mediated             expression cloning. Two types of interleukin-3 (IL-3)-dependent cell lines, Ba/F3             and HF6, were transduced with the cDNA libraries and several genes that render             these cells factor-independent were identified including PIM-1, PIM-2, PIM-3,             GADD45B and reproductive homeobox genes on the X chromosome gene F2 (RHOXF2).             Although no mutation in these genes was found, these molecules were highly expressed             in cancer cell lines and they may play important roles in cell transformation.             Among them, we focused on a transcriptional repressor RHOXF2. Transduction of             RHOXF2 rendered HF6 cells factor-independent, while knockdown of RHOXF2 inhibited             growth of the HGC27 gastric cancer cell line which highly expresses RHOXF2. In             addition, RHOXF2-transduced HF6 cells quickly induced leukemia when transplanted             into sublethally irradiated mice. Moreover, RHOXF2 is highly expressed in some             leukemia cell lines and a variety of human cancer samples including colon and             lung cancers. Thus, these results indicate that RHOXF2 is involved in carcinogenesis.
authors.affiliation: Division of Cellular Therapy, The Institute of Medical Science,                     The University of Tokyo, Tokyo 108-8639, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Shibata-Minoshima
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Oki
authors.forename: Noriko
authors.initials: N
authors.lastname: Doki
authors.forename: Fumio
authors.initials: F
authors.lastname: Nakahara
authors.forename: Shun-Ichiro
authors.initials: S
authors.lastname: Kageyama
authors.forename: Jiro
authors.initials: J
authors.lastname: Kitaura
authors.forename: Junya
authors.initials: J
authors.lastname: Fukuoka
authors.forename: Toshio
authors.initials: T
authors.lastname: Kitamura
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D018398:Homeodomain Proteins; C471968:RHOXF2 protein, human