title: The concentration of thiobarbituric acid reactive substances (TBARS) and paraoxonase activity in blood of patients with osteoarthrosis after endoprosthesis implantation.
abstract: BACKGROUND
  The aim of this study was to evaluate the concentration of malondialdehyde (MDA) in erythrocytes and in blood plasma and the activity of blood paraoxonase (PON1) of patients with osteoarthrosis (OA) submitted to endoprosthesis implantation for evaluating oxidative stress.
  
  
  METHODS
  Study was conducted on 55 patients with OA and on 54 total movement-efficient volunteers. The material for the study was venous blood plasma, serum and erythrocytes.
  
  
  RESULTS
  Increased concentration of MDAe before surgery was observed in the group of men and in patients with a degenerative process affecting hip joints. After an implantation of endoprosthesis, MDAe decreased to the level observed in the control groups. In the study group MDA concentration in plasma was slightly lower before surgery, and after an operation it reached the value of the parameter of the reference groups. Regardless of sex or age, paraoxonase activity was almost twice as high in almost all subgroups as in the reference group. A positive correlation between PON 1 activity and MDAe concentration was demonstrated both before and after surgery in the group of men.
  
  
  CONCLUSIONS
  The increase of PON1 activity in patients' serum in relation to the control groups indicates a probable pathogenic role of the increased formation of reactive oxygen species in the course of OA and may suggest acute inflammation of the synovial joint. The high level of PON 1 activity after endoprosthesis implantation indicates that surgical treatment may additionally stimulate ROS generation. MDAe concentration indicate more intensive process of lipid peroxidation in the elderly.
authors.affiliation: Department of Medical Biology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.
authors.forename: Dorota M
authors.initials: DM
authors.lastname: Olszewska-Słonina
authors.forename: Dariusz
authors.initials: D
authors.lastname: Mątewski
authors.forename: Rafał
authors.initials: R
authors.lastname: Czajkowski
authors.forename: Krzysztof J
authors.initials: KJ
authors.lastname: Olszewski
authors.forename: Alina
authors.initials: A
authors.lastname: Woźniak
authors.forename: Grażyna
authors.initials: G
authors.lastname: Odrowąż-Sypniewska
authors.forename: Kinga
authors.initials: K
authors.lastname: Lis
authors.forename: Dariusz
authors.initials: D
authors.lastname: Musiałkiewicz
authors.forename: Bogna
authors.initials: B
authors.lastname: Kowaliszyn
mesh_terms: D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D016022:Case-Control Studies; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood*; D019919:Prosthesis Implantation; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D043303:Aryldialkylphosphatase