title: Polymer brush controlled bioinspired calcium phosphate mineralization and bone cell growth.
abstract: Polymer brushes on thiol-modified gold surfaces were synthesized by using terminal thiol groups for the surface-initiated free radical polymerization of methacrylic acid and dimethylaminoethyl methacrylate, respectively. Atomic force microscopy shows that the resulting poly(methacrylic acid) (PMAA) and poly(dimethylaminoethyl methacrylate) (PDMAEMA) brushes are homogeneous. Contact angle measurements show that the brushes are pH-responsive and can reversibly be protonated and deprotonated. Mineralization of the brushes with calcium phosphate at different pH yields homogeneously mineralized surfaces, and preosteoblastic cells proliferate on both the nonmineralized and mineralized surfaces. The number of living cells on the mineralized hybrid surfaces is ca. 3 times (PDMAEMA) and 10 times (PMAA) higher than on the corresponding nonmineralized brushes.
authors.affiliation: Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str 24-25, D-14476 Golm, Germany.
authors.forename: Ruben
authors.initials: R
authors.lastname: Löbbicke
authors.forename: Munish
authors.initials: M
authors.lastname: Chanana
authors.forename: Helmut
authors.initials: H
authors.lastname: Schlaad
authors.forename: Christine
authors.initials: C
authors.lastname: Pilz-Allen
authors.forename: Christina
authors.initials: C
authors.lastname: Günter
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
authors.forename: Andreas
authors.initials: A
authors.lastname: Taubert
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D001842:Bone and Bones / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006046:Gold / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000138:chemical synthesis* / Q000494:pharmacology; D051379:Mice; D018625:Microscopy, Atomic Force; D009757:Nylons / Q000138:chemical synthesis* / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D055668:Photochemical Processes / Q000528:radiation effects; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis* / Q000494:pharmacology; D011522:Protons; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D008689:Methacrylates; D009757:Nylons; D011522:Protons; D013438:Sulfhydryl Compounds; C407037:poly(2-(dimethylamino)ethyl methacrylate); D006046:Gold; D019904:Polymethyl Methacrylate; C020243:calcium phosphate