title: Detection of ricin using a carbon nanofiber based biosensor.
abstract: We report ricin detection using antibody and aptamer probes immobilized on a nanoelectrode array (NEA) consisting of vertically aligned carbon nanofibers (VACNFs). These biosensor chips are fabricated on a wafer scale using steps common in integrated circuit manufacturing. Electrochemical impedance spectroscopy is used to characterize the detection event and the results indicate that the electron transfer resistance changes significantly after the ricin protein binds to the probe. Further confirmation is obtained from evaluation of the electrode surface by atomic force microscopy which clearly shows a change in height from the bare electrode to the surface bound by the probe-protein.
authors.affiliation: NASA Ames Research Center, Moffett Field, CA 94035, USA.
authors.forename: Adaikkappan
authors.initials: A
authors.lastname: Periyakaruppan
authors.forename: Prabhu U
authors.initials: PU
authors.lastname: Arumugam
authors.forename: M
authors.initials: M
authors.lastname: Meyyappan
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Koehne
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D058266:Dielectric Spectroscopy / Q000379:methods*; D018625:Microscopy, Atomic Force; D057139:Nanofibers / Q000737:chemistry*; D012276:Ricin / Q000032:analysis*; D052156:SELEX Aptamer Technique
chemical_list: D002244:Carbon; D012276:Ricin