title: Effective inhibition of irradiation on human gliomas growth in vitro and in vivo after epidermal growth factor receptor silencing with RNA interference.
abstract: In this study, we found that irradiation in the presence of small interfering RNA-epidermal growth factor receptor (EGFR) arrested U373 glioma cells in G₀ and G₁ phases, delayed cell cycle progression, and effectively inhibited cell proliferation compared with cells that received only radiotherapy. In addition, combined therapy enhanced the percent of apoptotic U373 cells in vitro and also reduced the tumor size and increased the survival rate in tumor xenograft studies. This study demonstrates the antitumor activity of ionizing radiation therapy in combination with small interfering RNA-EGFR in gliomas both in vitro and in vivo and provides a scientific rationale for targeting EGFR to enhance the sensitivity to radiotherapy in patients with glioblastoma multiforme.
authors.affiliation: Department of Radiology, Tangdu Hospital, Fourth Military Medical University, Xinsi Road, Xi'an, Shaan'xi, P.R. China. cuiguangbin@hotmail.com
authors.forename: Guangbin
authors.initials: G
authors.lastname: Cui
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Longxiao
authors.initials: L
authors.lastname: Wei
authors.forename: Pang
authors.initials: P
authors.lastname: Du
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Baoying
authors.initials: B
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Yin
authors.forename: Linfeng
authors.initials: L
authors.lastname: Yan
authors.forename: Jingguo
authors.initials: J
authors.lastname: Wei
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000528:radiation effects; D002469:Cell Separation; D003131:Combined Modality Therapy; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000235:genetics; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism / Q000532:radiotherapy*; D034622:RNA Interference; D034741:RNA, Small Interfering; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; D066246:ErbB Receptors