title: Assessment of HB-EGF levels in peritoneal fluid and serum of ovarian cancer patients using ELISA.
abstract: BACKGROUND
  Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target for ovarian cancer therapy. The aim of this study was to examine HB-EGF levels in the peritoneal fluid and serum of ovarian cancer (OVCA) patients.
  
  
  METHODS
  Samples were collected from six healthy women, 21 OVCA patients, and 21 ovarian cyst patients. HB-EGF levels were measured using a sandwich ELISA kit and calculated using a parallel line assay.
  
  
  RESULTS
  No significant difference between the slopes of the standard and sample curves was observed at an anti-HB-EGF antibody concentration of 1.6 μg/ml. HB-EGF levels in the peritoneal fluid and serum of OVCA patients were significantly higher than those in patients with ovarian cysts or controls. Serum HB-EGF levels were also significantly correlated with levels in peritoneal fluid in OVCA patients.
  
  
  CONCLUSIONS
  We developed an assay for the exact measurement of HB-EGF levels in peritoneal fluid and serum.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Hikita
authors.forename: Fusanori
authors.initials: F
authors.lastname: Yotsumoto
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukami
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fumaki
authors.forename: Shinji
authors.initials: S
authors.lastname: Horiuchi
authors.forename: Ayako
authors.initials: A
authors.lastname: Sanui
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyata
authors.forename: Sung Ouk
authors.initials: SO
authors.lastname: Nam
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujioka
authors.forename: Taeko
authors.initials: T
authors.lastname: Ueda
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shirota
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yoshizato
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Maeda
authors.forename: Toyokazu
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Okuno
authors.forename: Masahide
authors.initials: M
authors.lastname: Kuroki
authors.forename: Eisuke
authors.initials: E
authors.lastname: Mekada
authors.forename: Shingo
authors.initials: S
authors.lastname: Miyamoto
mesh_terms: D000328:Adult; D000368:Aged; D000906:Antibodies / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D016477:Artifacts; D001202:Ascitic Fluid / Q000737:chemistry*; D001667:Binding, Competitive; D014408:Biomarkers, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood / Q000276:immunology; D010048:Ovarian Cysts / Q000097:blood / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000097:blood / Q000378:metabolism*; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins