title: HIV-1 infection suppresses expression of host cell cycle-associated gene PDS5A.
abstract: OBJECTIVE
  To unravel the interplay between HIV-1 and its host cell, the effect of HIV-1 infection on cellular gene expression was investigated.
  
  
  METHODS
  HIV-1(SF33)-infected and uninfected H9 T cells were screened by differential display and RNase protection assay. The finding (PDS5A) was confirmed in HIV-1(Lai)-infected P4-CCR5 HeLa cells, which were also examined after PDS5A siRNA knockdown in regard to HIV-1 replication by quantitative RT-PCR, p24 ELISA and LTR-driven β-galactosidase expression. The PDS5A knockdown effect on cellular gene expressions was studied by microarray analysis. PDS5A tissue expression was determined by Northern blotting.
  
  
  RESULTS
  Regulator of cohesion maintenance, homolog A (PDS5A) was found to be down-regulated by HIV-1. When PDS5A was suppressed by siRNA, HIV-1 replication was unaffected. PDS5A was found to be highly expressed in skeletal muscle tissue, and to lesser degrees in pancreas, heart, placenta, lung, kidney, liver and brain. Microarray analysis of PDS5A knockdown revealed 91 differential gene products over-representing cell cycle, transport and protein stability regulation, including 4 genes (PP2A, RANTES, PCAF, TCF7L2) previously reported to interact with HIV-1.
  
  
  CONCLUSIONS
  The data show a downregulation of proliferation-associated host gene PDS5A and suggest a role of PDS5A in HIV-1-induced cellular pathogenesis but not viral replication.
authors.affiliation: Department of Hematology/Oncology and Infectious Diseases, Johann Wolfgang Goethe University Hospital, Frankfurt/Main, Germany.
authors.forename: Gianni
authors.initials: G
authors.lastname: Capalbo
authors.forename: Thea
authors.initials: T
authors.lastname: Müller-Kuller
authors.forename: Oliver G
authors.initials: OG
authors.lastname: Ottmann
authors.forename: Dieter
authors.initials: D
authors.lastname: Hoelzer
authors.forename: Urban J
authors.initials: UJ
authors.lastname: Scheuring
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology; D020869:Gene Expression Profiling; D015497:HIV-1 / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D046228:Microarray Analysis; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors*; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000821:virology
chemical_list: D009687:Nuclear Proteins; C486457:PDS5A protein, human