title: Photobacterium atrarenae sp. nov. a novel bacterium isolated from sea sand.
abstract: The gram-reaction-negative, motile, facultatively anaerobic, catalase-positive, oxidase-positive bacterial strain M3-4(T) was isolated from black sea sand and subjected to a taxonomic study. Cells of strain M3-4(T) have monotrichous flagella, grow optimally at 37°C and at pH 7-8 in the presence of 1-4% (w/v) NaCl and hydrolyze casein, starch and L: -tyrosine. According to phylogenetic analyses using 16S rRNA gene sequences, strain M3-4(T) belongs to the genus Photobacterium and is most closely related to Photobacterium rosenbergii LMG 22223(T) (97.4%) and P. gaetbulicola KCTC 22804(T) (96.6%). The DNA-DNA relatedness value between M3-4(T) and P. rosenbergii LMG 22223(T) was 21.5%. The DNA G+C mol% of strain M3-4(T) was 53.6. The major cellular fatty acid of strain M3-4(T) was a summed feature 3 consisting of C(16:1) ω7c and/or iso-C(15:0) 2-OH (35.0%), followed by C(16:0) (25.4%) and C(18:1)ω7c (16.8%). These data suggest that strain M3-4(T) represents a novel species in genus Photobacterium, for which the name P. atrarenae sp. nov. is proposed. The type strain is M3-4(T) (= KCTC 23265(T) = NCAIM B 02414(T)).
authors.affiliation: Microbial Resource Center, KRIBB, Daejeon 305-806, Republic of Korea.
authors.forename: Byung-Chun
authors.initials: BC
authors.lastname: Kim
authors.forename: Haryoung
authors.initials: H
authors.lastname: Poo
authors.forename: Mi Na
authors.initials: MN
authors.lastname: Kim
authors.forename: Kang Hyun
authors.initials: KH
authors.lastname: Lee
authors.forename: Jongtae
authors.initials: J
authors.lastname: Lee
authors.forename: Moon-Soo
authors.initials: MS
authors.lastname: Rhee
authors.forename: Kee-Sun
authors.initials: KS
authors.lastname: Shin
mesh_terms: D001482:Base Composition; D059038:Black Sea; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010776:Photobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D012822:Silicon Dioxide
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide