title: Cobalt phthalocyanine as a biomimetic catalyst in the amperometric quantification of dipyrone using FIA.
abstract: A biomimetic sensor for the determination of dipyrone was prepared by modifying carbon paste with cobalt phthalocyanine (CoPc), and used as an amperometric detector in a flow injection analysis (FIA) system. The results of cyclic voltammetry experiments suggested that CoPc behaved as a biomimetic catalyst in the electrocatalytic oxidation of dipyrone, which involved the transfer of one electron. The optimized FIA procedure employed a flow rate of 1.5 mL min(-1), a 75 μL sample loop, a 0.1 mol L(-1) phosphate buffer carrier solution at pH 7.0 and amperometric detection at a potential of 0.3 V vs. Ag/AgCl. Under these conditions, the proposed method showed a linear response for dipyrone concentrations in the range 5.0 × 10(-6)-6.3 × 10(-3) mol L(-1). Selectivity and interference studies were carried out in order to validate the system for use with pharmaceutical and environmental samples. In addition to being environmentally friendly, the proposed method is a sensitive and selective analytical tool for the determination of dipyrone.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, State University of São Paulo (UNESP), 14801-970 Araraquara, SP, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Boni
authors.forename: Ademar
authors.initials: A
authors.lastname: Wong
authors.forename: Rosa Amália Fireman
authors.initials: RA
authors.lastname: Dutra
authors.forename: Maria Del Pilar Taboada
authors.initials: Mdel P
authors.lastname: Sotomayor
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004177:Dipyrone / Q000032:analysis*; D004563:Electrochemistry / Q000191:economics / Q000379:methods*; D017022:Flow Injection Analysis / Q000191:economics / Q000379:methods*; D007211:Indoles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D013997:Time Factors
chemical_list: D007211:Indoles; D009942:Organometallic Compounds; C063633:cobalt phthalocyanine; D004177:Dipyrone; D003577:Cytochrome P-450 Enzyme System