title: Characterization of a novel molluscan MyD88 family protein from manila clam, Ruditapes philippinarum.
abstract: Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein which is required for signal transduction of TLR/IL-1R family. In this study, a novel molluscan MyD88 family member protein (named as RpMyD88) was identified from manila clam, Ruditapes philippinarum. It was identified using BLAST algorithm from GS-FLX™ sequencing data. The cDNA of RpMyD88 consists of 1416 bp open reading frame (ORF) encoding 471 amino acid residues. The RpMyD88 contains death domain and Toll/interleukin-1 receptor (TIR) domain which are typical features of MyD88 family proteins. The predicted amino acid sequence of RpMyD88 shares 27% identity with scallop MyD88. The expression level of RpMyD88 mRNA was investigated in healthy and challenged clams by quantitative real-time RT-PCR. The RpMyD88 gene expression is ubiquitous in all selected tissues. The RpMyD88 mRNA was strongly expressed in hemocyte, gill and mantle. In contrast, it was weakly expressed in siphon, foot and adductor muscle. RpMyD88 was up-regulated in gill and hemocyte after immune challenge with both Vibrio tapetis and LPS challenge. All results considered, sequence characterization, comparison and gene expression data suggesting that MyD88-dependent signaling pathway is presence in manila clam and RpMyD88 plays an important role in innate immune response against bacteria.
authors.affiliation: Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Special Self-Governing Province 690-756, Republic of Korea.
authors.forename: Youngdeuk
authors.initials: Y
authors.lastname: Lee
authors.forename: Ilson
authors.initials: I
authors.lastname: Whang
authors.forename: Navaneethaiyer
authors.initials: N
authors.lastname: Umasuthan
authors.forename: Mahanama
authors.initials: M
authors.lastname: De Zoysa
authors.forename: Chulhong
authors.initials: C
authors.lastname: Oh
authors.forename: Do-Hyung
authors.initials: DH
authors.lastname: Kang
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
authors.forename: Choul-Ji
authors.initials: CJ
authors.lastname: Park
authors.forename: Jehee
authors.initials: J
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014733:Vibrio / Q000276:immunology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88