title: Proteomic and immunoproteomic analysis of Aggregatibacter actinomycetemcomitans JP2 clone strain HK1651.
abstract: The proteome of Aggregatibacter actinomycetemcomitans HK1651 (JP2 clone) and immunoreactive antigens were studied by two-dimensional (2D) gel electrophoresis, matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS), and 2D immunoblotting. The highly leukotoxic JP2 clone of A. actinomycetemcomitans is strongly associated with aggressive periodontitis (AgP) in adolescents of North-West African descent and the pathogenicity of this bacterium is of major interest. Hence, we developed a comprehensive 2D proteome reference map of A. actinomycetemcomitans proteins with 167 identified spots representing 114 different proteins of which 15 were outer membrane proteins. To unravel immunoreactive antigens, we applied 2D-gel and subsequent immunoblotting analyses using sera from five individuals with A. actinomycetemcomitans infections and one healthy control. The analysis revealed 32 immunoreactive proteins. Antibodies to two outer membrane proteins, YaeT (85 kDa) and Omp39 (39 kDa), not previously described as immunoreactive, were found only in subjects with current or previous A. actinomycetemcomitans JP2 infection. Further proteome-based studies of A. actinomycetemcomitans combined with analyses of the humoral immune response and targeted against outer membrane proteins may provide important insight into the host relationship of this important pathogen.
authors.affiliation: Department of Medical Microbiology and Immunology, School of Dentistry, Faculty of Health Sciences, Aarhus University, Aarhus, Denmark. rylev@microbiology.au.dk
authors.forename: Mette
authors.initials: M
authors.lastname: Rylev
authors.forename: Ahmed B
authors.initials: AB
authors.lastname: Abduljabar
authors.forename: Jesper
authors.initials: J
authors.lastname: Reinholdt
authors.forename: Oum-Keltoum
authors.initials: OK
authors.lastname: Ennibi
authors.forename: Dorte
authors.initials: D
authors.lastname: Haubek
authors.forename: Svend
authors.initials: S
authors.lastname: Birkelund
authors.forename: Mogens
authors.initials: M
authors.lastname: Kilian
mesh_terms: D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007166:Immunosuppressive Agents / Q000378:metabolism*; D015661:Pasteurellaceae / Q000276:immunology / Q000378:metabolism* / Q000472:pathogenicity; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007166:Immunosuppressive Agents