title: Delayed ischemic postconditioning protects hippocampal CA1 neurons by preserving mitochondrial integrity via Akt/GSK3β signaling.
abstract: Delayed ischemic postconditioning (Post C), which involves a brief ischemia followed by reperfusion 2 days after 8-10min global cerebral ischemia (GCI), has been shown to exert a remarkable protection of the vulnerable hippocampal CA1 region of the brain and attenuation of behavioral deficits, although the mechanisms remain poorly understood. The purpose of the current study was to explore the effect of Post C upon mitochondrial integrity, cytochrome c release and Bax translocation as a potential key mechanism for Post C protection of the critical hippocampal CA1 region neurons. The results of the study revealed that ischemic Post C (3min) administered 2 days after 8-min GCI exerted a robust preservation from GCI injury, as evidenced by the increase of NeuN-positive and the decrease of TUNEL-positive cells, as well as morphological features of mitochondrial integrity in the hippocampal CA1 region. We also found that Post C significantly blocked inner mitochondrial membrane potential depolarization, as shown by JC-1 staining, and attenuates cytochrome c release and Bax translocation induced by GCI. Pre-treatment of the PI3K inhibitor LY294002, 20min prior to Post C, significantly attenuated Post C-induced elevation of p-Akt and p-GSK3β, as well as prevented Post C enhancement of mitochondrial integrity and Post C neuroprotection. The results suggest that phosphorylation of Akt and subsequent inactivation of GSK3β signaling is critical in mediating Post C beneficial effects upon mitochondrial integrity, function and neuroprotection following GCI injury.
authors.affiliation: Institute of Neurobiology, Experimental and Research Center, Hebei United University, 57 South Jianshe Road, Tangshan, Hebei Province 063600, China.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Zhou
authors.forename: Jingyi
authors.initials: J
authors.lastname: Tu
authors.forename: Quanguang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Dongshuang
authors.initials: D
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Darrell W
authors.initials: DW
authors.lastname: Brann
authors.forename: Ruimin
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D020520:Brain Infarction / Q000473:pathology / Q000517:prevention & control / Q000628:therapy*; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy; D056547:CA1 Region, Hippocampal / Q000098:blood supply / Q000378:metabolism* / Q000503:physiopathology; D038362:Glycogen Synthase Kinase 3 / Q000502:physiology*; D000071679:Glycogen Synthase Kinase 3 beta; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009410:Nerve Degeneration / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D018696:Neuroprotective Agents; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D051057:Proto-Oncogene Proteins c-akt; D038362:Glycogen Synthase Kinase 3