title: Maspin expression is regulated by the non-canonical NF-κB subunit in androgen-insensitive prostate cancer cell lines.
abstract: Dysregulation of Maspin expression and constitutive activation of NF-κB subunits are important events in tumorigenesis of prostate cancer. Recent finding points that RelB, which contributes to the alternative NF-κB activity, interferes with carcinogenesis in the prostate. We report here, that both the classical and the alternative NF-κB activities are constitutively present in androgen-insensitive human prostate cancer cells. Maspin and RelB expression is correlated negatively in prostate cancer tissues at the later stage. TNF-α signaling triggers the nuclear accumulation of RelB and the concomitant reduction of Maspin expression in a time-dependent manner. In addition, the proteasome inhibitor-induced Maspin expression is accompanied by the reduction of RelB expression. A successful depletion of RelB expression, but not RelA expression, induces Maspin expression. RelB-deficiency abrogates the proteasome inhibitor-induced Maspin expression. Moreover, we demonstrate that the enforced expression of RelB protein in prostate cancer cells inhibits Maspin expression. We propose that RelB is an essential molecule controlling the endogenous and the proteasome inhibitor-induced Maspin expression. Developing a RelB-targeted therapeutic intervention, which might be coupled with the induction of a tumor suppressor Maspin, is valuable in treating advanced, metastatic prostate cancer.
authors.affiliation: Central Lab, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. guofeng27@suda.edu.cn
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Suya
authors.initials: S
authors.lastname: Kang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Linchuan
authors.initials: L
authors.lastname: Guo
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Jianquan
authors.initials: J
authors.lastname: Hou
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D015843:Serpins / Q000096:biosynthesis* / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics*; D051996:Transcription Factor RelA / Q000235:genetics* / Q000378:metabolism; D051841:Transcription Factor RelB / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; C085344:SERPIN-B5; D015843:Serpins; D051996:Transcription Factor RelA; D051841:Transcription Factor RelB