title: Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development.
abstract: Non-coding RNAs (ncRNAs) Meg8 and Irm were previously identified as alternatively splicing isoforms of Rian gene. Ascertaining ncRNAs spatiotemporal expression patterns is crucial for understanding the physiological roles of ncRNAs during tissue and organ development. In this study in mouse embryos, we focused on the developmental regulation expression of imprinted macro ncRNAs, Meg8 and Irm by using in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). The in situ hybridization results showed that Meg8 and Irm were expressed in the developing brain at embryonic day 10.5 (E10.5) and E11.5, while Irm expression signals were strikingly detected in the somite, where Meg8 expression signals were undetectable. By E15.5, they were expressed in brain, tongue, liver, lung and neuroendocrine tissues, while Irm displayed more restricted expression in tongue and skeletal muscle than Meg8. Furthermore, quantitative analysis confirmed that they were highly expressed in tongue and brain at E12.5, E15.5 and E18.5. These results indicated that Meg8 and Irm might be coordinately expressed and functionally correlated in diverse of organs. Notably, Irm was more closely associated with morphogenesis of skeletal muscle in contrast to Meg8 during embryonic development.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Department of Life Science and Engineering, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Gu
authors.forename: Hongjuan
authors.initials: H
authors.lastname: He
authors.forename: Zhengbin
authors.initials: Z
authors.lastname: Han
authors.forename: Tiebo
authors.initials: T
authors.lastname: Zeng
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Huang
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Huijie
authors.initials: H
authors.lastname: Jiang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D011247:Pregnancy; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014059:Tongue / Q000196:embryology / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D022661:RNA, Untranslated; C441460:Rian protein, mouse