title: Resonance light scattering method for the determination of DNA with cationic methacrylate based polymer nanoparticle probes.
abstract: Narrowly distributed cationic poly (methyl methacrylate-co-diacetone acrylamide) (P(MMA-DAAM)) nanoparticles were successfully prepared by microemulsion polymerization. Photon correlation spectrometer (PCS) measurement and transmission electron microscope (TEM) observation revealed that z-average particle size of P(MMA-DAAM) is ∼27.5 nm. It was found that these cationic nanoparticles interact with DNA through electrostatic interaction to form P(MMA-DAAM)-DNA complex, which significantly enhances the resonance light scattering (RLS) signal. Therefore, a novel method using this polymer nanoparticle as a new probe for the detection of DNA by RLS technique is developed in this paper. The results showed this method is very convenient, sensitive, and reproducible.
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineer, Hubei University, Wuhan 430062, PR China. qichaozou@yahoo.com.cn
authors.forename: Qi-Chao
authors.initials: QC
authors.lastname: Zou
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Zhang
authors.forename: Shi-Gan
authors.initials: SG
authors.lastname: Chai
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D004655:Emulsions; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D017785:Photons; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000178:Acrylamides; D002412:Cations; D004655:Emulsions; D011108:Polymers; C569451:poly (methyl methacrylate-co-diacetone acrylamide); D004247:DNA; D019904:Polymethyl Methacrylate