title: Re-expression of estrogen receptor β inhibits the proliferation and             migration of ovarian clear cell adenocarcinoma cells.
abstract: Ovarian clear cell adenocarcinoma (OCCA) is an aggressive ovarian malignancy             with a poor prognosis. The role of estrogen receptor β (ERβ) in the development             of OCCA remains to be clarified. To investigate the action of ERβ in the proliferation             and invasion of OCCA cells, the ES-2 cell line was stably transfected with full-length             human ERβ cDNA, and clones were screened and identified using RT-PCR and western             blot assay. ERβ stable transfectants, referred to as ESβ1 and ESβ2 cells, were             compared with mock transfectant ESVE and parental ES-2 cells with respect to their             growth, motility and ability to activate target genes. ESβ1 and ESβ2 cells expressed             ERβ mRNA and protein, whereas ES-2 and ESVE cells were ERβ negative. ERβ transfectants             exhibited distinct characteristics from ES-2 and ESVE cells including proliferative             properties and the ability to express cyclin D1 in the presence of 17β-estradiol             (E2). ERβ inhibited ES-2 cell proliferation, which was determined using the MTT             assay, BrdU labeling method and by the down-regulation of cyclin D1 gene expression.             Moreover, exogenous ERβ expression resulted in a significant inhibition of ES-2             cell motility in an in vitro invasion assay. ERβ reduced the expression of MMP2             mRNA and the activity of MMP2 enzymatic activity in a ligand-dependent manner.             In summary, ERβ may inhibit the proliferation and invasion of ES-2 cells and may             be an important regulator in OCCA carcinogenesis.
authors.affiliation: Obstetrics and Gynecology Hospital at Fudan University, Shanghai 200011, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Keqin
authors.initials: K
authors.lastname: Hua
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Yu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jin
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
mesh_terms: D018262:Adenocarcinoma, Clear Cell; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010051:Ovarian Neoplasms; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C530104:CCND1 protein, human; D047629:Estrogen Receptor beta; D011994:Recombinant Proteins; D019938:Cyclin D1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2