title: The influence of corticosterone on antizyme gene expression in early regenerating rat liver.
abstract: OBJECTIVE
  Antizyme 1, a specific inhibitor of Ornithine decarboxylase (ODC), plays a critical role in cell proliferation. Little is known about the impact of glucocorticoid on antizyme expression in the regenerating liver. In this paper, the effect of corticosterone on the gene expression of antizyme 1 in early regenerating rat liver induced by partial hepatectomy (PH) was investigated.
  
  
  METHODS
  Bilateral adrenalectomies (ADX) were performed 3 days before PH. Corticosterone in sesame oil or sesame oil was injected sub-cutaneously to ADX rats. Antizyme 1 mRNA and protein levels as well as polyamine contents in the regenerating liver were determined by RT-PCR, Western blotting and HPLC, respectively.
  
  
  RESULTS
  Antizyme 1 protein content in the oil-treated ADX group decreased significantly at 5, 7 and 9 h after PH compared to control. Following corticosterone administration the content rose dose-dependently during the whole experiment. At 5 h post-PH, the protein levels in 10 and 40 mg/kg corticosterone-treated ADX rats increased by 66% and 148%, respectively, when compared with the control group. However, no significant changes in antizyme 1 mRNA levels were observed in oil-treated ADX rats or corticosterone-treated groups compared to control. Polyamine contents in oil-treated ADX rats were the highest among all groups at 5 and 9 h. Corticosterone treatment resulted in a dramatic decrease of polyamine contents at most of the time points investigated when compared with those in control rats.
  
  
  CONCLUSIONS
  Corticosterone treatment induces antizyme 1 protein synthesis in early regenerating rat liver. However, it has little effect on antizyme 1 gene transcription. (Acta gastroenterol. belg., 2011, 74, 289-294).
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: J F
authors.initials: JF
authors.lastname: Dong
authors.forename: Q J
authors.initials: QJ
authors.lastname: Ning
authors.forename: J K
authors.initials: JK
authors.lastname: Li
mesh_terms: D000818:Animals; D003345:Corticosterone / Q000494:pharmacology*; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors; D005786:Gene Expression Regulation / Q000187:drug effects*; D006498:Hepatectomy; D008115:Liver Regeneration / Q000187:drug effects / Q000235:genetics*; D008297:Male; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012313:RNA / Q000235:genetics*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D011506:Proteins; C031017:ornithine decarboxylase antizyme; D012313:RNA; D003345:Corticosterone