title: Analysis of KIT expression and KIT exon 11 mutations in canine oral malignant melanomas.
abstract: KIT, a transmembrane receptor tyrosine kinase, is one of the specific targets for anti-cancer therapy. In humans, its expression and mutations have been identified in malignant melanomas and therapies using molecular-targeted agents have been promising in these tumours. As human malignant melanoma, canine malignant melanoma is a fatal disease with metastases and the poor response has been observed with all standard protocols. In our study, KIT expression and exon 11 mutations in dogs with histologically confirmed malignant oral melanomas were evaluated. Although 20 of 39 cases were positive for KIT protein, there was no significant difference between KIT expression and overall survival. Moreover, polymerase chain reaction amplification and sequencing of KIT exon 11 in 17 samples did not detect any mutations and proved disappointing. For several reasons, however, KIT expression and mutations of various exons including exon 11 should be investigated in more cases.
authors.affiliation: Laboratory of Veterinary Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Yanai
authors.forename: Y
authors.initials: Y
authors.lastname: Hoshino
authors.forename: K
authors.initials: K
authors.lastname: Maruo
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis / Q000662:veterinary; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D004285:Dogs; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D007150:Immunohistochemistry / Q000662:veterinary; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D009154:Mutation; D016133:Polymerase Chain Reaction; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics* / Q000378:metabolism
chemical_list: D019009:Proto-Oncogene Proteins c-kit