title: The effect of stinging nettle (Urtica dioica) seed oil on experimental colitis in rats.
abstract: This study investigated the effect of Urtica dioica, known as stinging nettle, seed oil (UDO) treatment on colonic tissue and blood parameters of trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Experimental colitis was induced with 1 mL of TNBS in 40% ethanol by intracolonic administration with a 8-cm-long cannula with rats under ether anesthesia, assigned to a colitis group and a colitis+UDO group. Rats in the control group were given saline at the same volume by intracolonic administration. UDO (2.5 mL/kg) was given to the colitis+UDO group by oral administration throughout a 3-day interval, 5 minutes later than colitis induction. Saline (2.5 mL/kg) was given to the control and colitis groups at the same volume by oral administration. At the end of the experiment macroscopic lesions were scored, and the degree of oxidant damage was evaluated by colonic total protein, sialic acid, malondialdehyde (MDA), and glutathione levels, collagen content, tissue factor activity, and superoxide dismutase and myeloperoxidase activities. Colonic tissues were also examined by histological and cytological analysis. Pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β, and interleukin-6), lactate dehydrogenase activity, and triglyceride and cholesterol levels were analyzed in blood samples. We found that UDO decreased levels of pro-inflammatory cytokines, lactate dehydrogenase, triglyceride, and cholesterol, which were increased in colitis. UDO administration ameliorated the TNBS-induced disturbances in colonic tissue except for MDA. In conclusion, UDO, through its anti-inflammatory and antioxidant actions, merits consideration as a potential agent in ameliorating colonic inflammation.
authors.affiliation: Department of Biochemistry, Marmara University, Istanbul, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Genc
authors.forename: Aysen
authors.initials: A
authors.lastname: Yarat
authors.forename: Tugba
authors.initials: T
authors.lastname: Tunali-Akbay
authors.forename: Goksel
authors.initials: G
authors.lastname: Sener
authors.forename: Sule
authors.initials: S
authors.lastname: Cetinel
authors.forename: Rabia
authors.initials: R
authors.lastname: Pisiriciler
authors.forename: Esin
authors.initials: E
authors.lastname: Caliskan-Ak
authors.forename: Ayhan
authors.initials: A
authors.lastname: Altıntas
authors.forename: Betul
authors.initials: B
authors.lastname: Demirci
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy; D015850:Interleukin-6 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity; D026982:Urtica dioica / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D010938:Plant Oils; D014280:Triglycerides; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019158:N-Acetylneuraminic Acid