title: [Experimental studies on the early treatment of soft tissue explosion injury by vacuum-assisted closure].
abstract: OBJECTIVE
  To investigate the effect on early treatment with vacuum-assisted closure(VAC) to wound healing of acute explosion injury in pigs, and provide a new way for early treatment of battle wounds.
  
  
  METHODS
  Eight healthy 3-month Landrace pigs of both sexes with the body mass of (50 +/- 5) kg were selected in the study. Sixteen battle wounds were made by explosion of same type detonator (pattern number: 660929F48840-55, included DDNP 0.3 g, RDX 0.7 g) in hibateral skin of buttock of 8 pigs, which were divided into experimental group and control group (pair wounds of left and right). The raw sufaces were thorough debrided at 3 h after exposure, according to the characteristics of treatment on the battlefield, experimental group was treated with VAC under the pressure of (-50 +/- 5) Kpa after debridement and sterilization and control group was treated with routine dry sterile gauze draping. Results of bacteriology (bacterial counts and the proportion of G+ bacteria) and pathology (HE stain and Masson stain) were detected at every wound before and after treatment.
  
  
  RESULTS
  At the 3 days after treatment,the bacterial number in the experimental group was [(7.82 +/- 0.55) x 10(4) ] CFU/g, in control group was [(1.07 +/- 0.14) x 10(6)] CFU/g. There was significant difference between two groups. The proportion of G+ bacteria in experimental group was significantly increased. The raw surface in experimental group was clean with affluent and neoformative granulation tissue, blood vessels and collagen, necrotic tissue decreased obviously by pathological observation.
  
  
  CONCLUSIONS
  VAC could reduce the quantity of bacteria, improve the proportion of G+ bacteria, and promote the formation of granulation tissue and the healing of wound. The VAC for the treatment of battle wounds has a positive effect.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou Command of Chinese PLA, Lanzhou 730050, Gansu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.forename: Bao-feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Chen
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: Zhao
authors.forename: Gui-qiu
authors.initials: GQ
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D005107:Explosions; D005260:Female; D008297:Male; D054843:Negative-Pressure Wound Therapy / Q000379:methods*; D017695:Soft Tissue Injuries / Q000209:etiology* / Q000382:microbiology / Q000473:pathology / Q000601:surgery*; D013194:Staining and Labeling; D013552:Swine; D013997:Time Factors