title: Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm.
abstract: The objective of this study was to screen for novel quorum-sensing inhibitors (QSIs) from traditional Chinese medicines (TCMs) that inhibit bacterial biofilm formation. Six of 46 active components found in TCMs were identified as putative QSIs based on molecular docking studies. Of these, three compounds inhibited biofilm formation by Pseudomonas aeruginosa and Stenotrophomonas maltophilia at a concentration of 200 µM. A fourth compound (emodin) significantly inhibited biofilm formation at 20 µM and induced proteolysis of the quorum-sensing signal receptor TraR in Escherichia coli at a concentration of 3-30 mM. Emodin also increased the activity of ampicillin against P. aeruginosa. Therefore, emodin might be suitable for development into an antivirulence and antibacterial agent.
authors.affiliation: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.|State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Xian
authors.initials: X
authors.lastname: Ding
authors.affiliation: LED, South China Sea Institute of Oceanology, Chinese Academy of Science, Guangzhou 510301, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yin
authors.affiliation: School of Chemistry and Chemical Engineering, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Qian
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Zeliang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, PR China.
authors.forename: Shining
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000880:Anthraquinones / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D003374:Coumarins / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004642:Emodin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009285:Naphthoquinones / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D053038:Quorum Sensing / Q000187:drug effects*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D009285:Naphthoquinones; C016101:shikonin; C001684:marmesin; D004642:Emodin; C027113:chrysophanic acid; C080614:fraxin; C020491:rhein