title: GeBP/GPL transcription factors regulate a subset of CPR5-dependent processes.
abstract: The CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES5 (CPR5) gene of Arabidopsis (Arabidopsis thaliana) encodes a putative membrane protein of unknown biochemical function and displays highly pleiotropic functions, particularly in pathogen responses, cell proliferation, cell expansion, and cell death. Here, we demonstrate a link between CPR5 and the GLABRA1 ENHANCER BINDING PROTEIN (GeBP) family of transcription factors. We investigated the primary role of the GeBP/GeBP-like (GPL) genes using transcriptomic analysis of the quadruple gebp gpl1,2,3 mutant and one overexpressing line that displays several cpr5-like phenotypes including dwarfism, spontaneous necrotic lesions, and increased pathogen resistance. We found that GeBP/GPLs regulate a set of genes that represents a subset of the CPR5 pathway. This subset includes genes involved in response to stress as well as cell wall metabolism. Analysis of the quintuple gebp gpl1,2,3 cpr5 mutant indicates that GeBP/GPLs are involved in the control of cell expansion in a CPR5-dependent manner but not in the control of cell proliferation. In addition, to our knowledge, we provide the first evidence that the CPR5 protein is localized in the nucleus of plant cells and that a truncated version of the protein with no transmembrane domain can trigger cpr5-like processes when fused to the VP16 constitutive transcriptional activation domain. Our results provide clues on how CPR5 and GeBP/GPLs play opposite roles in the control of cell expansion and suggest that the CPR5 protein is involved in transcription.
authors.affiliation: Institut Albert Bonniot, Institut National de la Santé et de la Recherche Médicale/Université Joseph Fourier U823, Equipe Interference ARN et Epigenetique, Rond-point de la Chantourne, 38706 La Tronche cedex, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Perazza
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laporte
authors.forename: Claudine
authors.initials: C
authors.lastname: Balagué
authors.forename: Florian
authors.initials: F
authors.lastname: Chevalier
authors.forename: Shanterika
authors.initials: S
authors.lastname: Remo
authors.forename: Mickaël
authors.initials: M
authors.lastname: Bourge
authors.forename: John
authors.initials: J
authors.lastname: Larkin
authors.forename: Michel
authors.initials: M
authors.lastname: Herzog
authors.forename: Gilles
authors.initials: G
authors.lastname: Vachon
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D048429:Cell Size / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004843:Epistasis, Genetic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D019441:Plant Epidermis / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D021381:Protein Transport / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; C528008:CPR5 protein, Arabidopsis; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; C000629467:STKR1 protein, Arabidopsis; D014157:Transcription Factors; D016590:Aphidicolin