title: [Mechanism of the effects of amitrole on the thyroglobulin in Fischer rat thyroid follicle-5 cell].
abstract: OBJECTIVE
  The mechanisms of the effects of amitrole on the thyroglobulin (TG) was investigated in Fischer rat thyroid follicle-5 cell (FRTL-5 cells) and in the medium.
  
  
  METHODS
  FRTL-5 cells were treated with 1, 10 and 100 microg/ml amitrole, and cytotoxicity was tested by 3H-TdR. The effects of amitrole on TG and thyroid transcription factor 1(TTF-1) in FRTL-5 cells were analyzed by RIA and ICC. And the TSHR in FRTL-5 cells was examined by Immunofluorescence analysis.
  
  
  RESULTS
  1,10 and 100 microg/ml amitrole had no significant cytotoxicity to the FRTL-5 cells (P > 0.05). The concentration of TG in the culture was decreased by 10 and 100 microg/ml amitrole (P < 0.05), and the concentration of TG in the cells was decreased by 100 microg/ml (P < 0.05) but the TTF-1 in the cells were not obviously changed (P > 0.05). The TSHR in the surface of FRTL-5 cells was significantly decreased by all doses of amitrole (P < 0.01).
  
  
  CONCLUSIONS
  The influence of amitrole on TG of FRTL-5 cells may be related to significantly reduce TSHR in the surface of FRTL-5 cells.
authors.affiliation: Institute of Health Science, Kunming Medical College, Kunming 650031, China. happyphm@163.com
authors.forename: Hongmei
authors.initials: H
authors.lastname: Pan
authors.forename: Lishi
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000640:Amitrole / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology; D006540:Herbicides / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D013954:Thyroglobulin / Q000032:analysis / Q000187:drug effects*; D013961:Thyroid Gland / Q000737:chemistry* / Q000166:cytology*
chemical_list: D006540:Herbicides; D013954:Thyroglobulin; D000640:Amitrole