title: [Experimental research on the influence of stress factors in an animal model of hypertension].
abstract: OBJECTIVE
  to investigate the influence of some stress factors in endothelin-1-induced hypertension.
  
  
  METHODS
  The experiment was carried out on Wistar rats, treated intraperitoneally, for 4 weeks, as follows: Group 1: saline solution (0.5 ml/100 g weight); Group 2: saline solution + stress; Group 3: Endotelin-1; Group 4: Endotelin-l + stress; Group 5: Endotelin-1 + cholesterol diet (0.2 g/kbw/day); Group 6: Endotelin-1 + cholesterol diet + stress. Endotelin-1 0.25 nmol/kbw was initially administered, followed by 0.5 nmol/kbw 15 min later, 2 times/week. Stress-inducing factors were immobilization and water immersion. In the 28th day of the experiment blood pressure was measured and blood samples were taken from the retro-orbitary plexus to assess plasma cortisol, blood count, phagocytic capacity of peripheral neutrophils, and serum complement activity.
  
  
  CONCLUSIONS
  Repeated administration of endotelin-1 determined an increase in blood pressure, statistically significant in stress conditions comparing to non-stressed animals. Our study proved a decrease of plasma cortisol, total leukocyte count, phagocytic capacity of peripheral neutrophils, without significant alterations in serum complement activity.
  
  
  CONCLUSIONS
  Chronic exposure to complex stress conditions in rats with endothelin-1-induced hypertension determined a decrease of plasma cortisol levels, effect correlated with elevated blood pressure and decrease in the number and phagocytic function of peripheral neutrophils.
authors.affiliation: Universităţii de Medicină şi Farmacie Gr. T. Popa Iaşi, Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi, Facultatea de Medicină.
authors.forename: C
authors.initials: C
authors.lastname: Diaconu
authors.forename: Liliana
authors.initials: L
authors.lastname: Tarţău
authors.forename: Cătălina-Elena
authors.initials: CE
authors.lastname: Lupuşoru
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000008:administration & dosage* / Q000009:adverse effects; D006854:Hydrocortisone / Q000097:blood; D006973:Hypertension / Q000097:blood / Q000503:physiopathology* / Q000523:psychology; D007101:Immersion / Q000009:adverse effects; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013312:Stress, Physiological; D013315:Stress, Psychological; D014867:Water
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D019332:Endothelin-1; D014867:Water; D006854:Hydrocortisone