title: Visualizing receptor endocytosis and trafficking.
abstract: G-protein-coupled 7 transmembrane domain receptors (GPCR-7TMR) represent the largest class of membrane protein drug targets. They respond to a plethora of ligands ranging from small molecules to polypeptide hormones. Upon activation, almost all GPCR-7TMRs undergo desensitization followed by receptor internalization and resensitization. This cycle is crucially important for regulating the signal emanating from the receptor and is tightly linked to the receptor and/or the ligands studied. In this chapter, we describe some of the technical approaches that can be used to study GPCR internalization and trafficking.
authors.affiliation: Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
authors.forename: Ali
authors.initials: A
authors.lastname: Salahpour
authors.forename: Larry S
authors.initials: LS
authors.lastname: Barak
mesh_terms: D000818:Animals; D002460:Cell Line; D004705:Endocytosis; D005455:Fluorescent Antibody Technique / Q000379:methods*; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D006801:Humans; D018613:Microscopy, Confocal / Q000379:methods*; D021381:Protein Transport; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D043562:Receptors, G-Protein-Coupled; D049452:Green Fluorescent Proteins