title: Caffeic acid phenethyl ester modulates gentamicin-induced oxidative nephrotoxicity in kidney of rats.
abstract: In this study, the modulator effect of caffeic acid phenethyl ester (CAPE) on the oxidative nephrotoxicity of gentamicin in the kidneys of rats was investigated by determining indices of lipid peroxidation and the activities of antioxidant enzymes as well as by histological analyses. Forty female Wistar albino rats were randomly divided into four groups, namely control, gentamicin, CAPE, and gentamicin plus CAPE. On the 12th day of the study, all rats were sacrificed and then blood samples and kidneys were taken. Lipid peroxidation and nitric oxide levels, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) enzyme activities, and histological evaluation were measured in kidneys of rats. Levels of blood urea nitrogen and creatinine were studied in serum. CAPE with gentamicin caused decreases in lipid peroxidation, nitric oxide, urea nitrogen, and creatinine levels, although it caused increases in CAT, GSH-Px, and SOD activities when compared with gentamicin alone. In addition, on histological evaluation, the renal damage caused by gentamicin alone appeared much higher than that caused by CAPE plus gentamicin. It is concluded that oxidative stress plays a critical role in causing gentamicin nephrotoxicity and that this nephrotoxicity may be significantly reduced by CAPE.
authors.affiliation: Department of Infectious Diseases, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Fadime Ozge
authors.initials: FO
authors.lastname: Aygün
authors.forename: Füsun Zeynep
authors.initials: FZ
authors.lastname: Akçam
authors.forename: Onur
authors.initials: O
authors.lastname: Kaya
authors.forename: Betül Mermi
authors.initials: BM
authors.lastname: Ceyhan
authors.forename: Recep
authors.initials: R
authors.lastname: Sütçü
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects* / Q000494:pharmacology; D002109:Caffeic Acids / Q000494:pharmacology*; D003404:Creatinine / Q000378:metabolism; D005260:Female; D005839:Gentamicins / Q000009:adverse effects* / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000900:Anti-Bacterial Agents; D002109:Caffeic Acids; D005839:Gentamicins; D009569:Nitric Oxide; D003404:Creatinine; D010088:Oxidoreductases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol