title: Evidence for self-association of a miniaturized version of agrin from hydrodynamic and small-angle X-ray scattering measurements.
abstract: Hydrodynamic studies of miniagrin indicate a molar mass that is 20% larger than the value calculated from the sequence of this genetically engineered protein. Consistent with this finding is the negative sign and also the magnitude of the second virial coefficient obtained from small-angle X-ray scattering measurements. The inference that miniagrin reversibly self-associates is confirmed by a sedimentation equilibrium study that yields an equilibrium constant of 0.24 L/g for a putative monomer-dimer interaction. Finally, Guinier analysis of the small-angle X-ray scattering (SAXS) results yields concentration-dependent values for the radius of gyration that may be described by the monomer-dimer model and respective R(g) values of 40 and 105 Å for the monomeric and dimeric miniagrin species. Although intermolecular protein interactions are endemic in the events leading to acetylcholine receptor aggregation by agrin, the matrix proteoglycan of which miniagrin is a miniaturized model, this investigation raises the possibility that agrin may itself self-associate.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada. patelt@cc.umanitoba.ca
authors.forename: Trushar R
authors.initials: TR
authors.lastname: Patel
authors.forename: Tabot M D
authors.initials: TM
authors.lastname: Besong
authors.forename: Nehal
authors.initials: N
authors.lastname: Patel
authors.forename: Markus
authors.initials: M
authors.lastname: Meier
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Harding
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Winzor
authors.forename: Jörg
authors.initials: J
authors.lastname: Stetefeld
mesh_terms: D018171:Agrin / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D057809:HEK293 Cells; D006801:Humans; D057446:Hydrodynamics; D008958:Models, Molecular; D008970:Molecular Weight; D010957:Plasmids; D015202:Protein Engineering; D055503:Protein Multimerization; D053838:Scattering, Small Angle; D014162:Transfection; D014461:Ultracentrifugation
chemical_list: D018171:Agrin