title: A study of the genetic variability of human respiratory syncytial virus (HRSV) in Cambodia reveals the existence of a new HRSV group B genotype.
abstract: Human respiratory syncytial virus (HRSV) is the leading cause of hospitalization of children aged <5 years due to respiratory illness in industrialized countries, and pneumonia is the leading cause of mortality among children aged <5 years worldwide. Although HRSV was first identified in 1956, a preventative vaccine has yet to be developed. Here we report the results of the first study to investigate the circulation and genetic diversity of HRSV in Cambodia among an all-ages population over 5 consecutive years. The incidences of HRSV infection among all-ages outpatient and hospitalized populations were equivalent, at 9.5% and 8.2%, respectively. Infection was most prevalent among children aged <5 years, with bronchiolitis being the most frequently observed clinical syndrome in the same age group. Circulation of HRSV was seasonal, typically coinciding with the rainy season between July and November annually. Strains belonging to HRSV groups A and B were detected with equivalent frequencies; however, we observed a potentially biennial shift in the predominant circulating HRSV genotype. The majority of HRSV group B strains belonged to the recently described BA genotype, with the exception of 10 strains classified as belonging to a novel HRSV group B genotype, SAB4, first reported here.
authors.affiliation: Institut Pasteur in Cambodia, Réseau International des Instituts Pasteur, 5 Monivong Blvd., P.O. Box 983, Phnom Penh, Cambodia.
authors.forename: Alicia
authors.initials: A
authors.lastname: Arnott
authors.forename: Sirenda
authors.initials: S
authors.lastname: Vong
authors.forename: Sek
authors.initials: S
authors.lastname: Mardy
authors.forename: Simon
authors.initials: S
authors.lastname: Chu
authors.forename: Monica
authors.initials: M
authors.lastname: Naughtin
authors.forename: Ly
authors.initials: L
authors.lastname: Sovann
authors.forename: Carole
authors.initials: C
authors.lastname: Buecher
authors.forename: Julien
authors.initials: J
authors.lastname: Beauté
authors.forename: Sareth
authors.initials: S
authors.lastname: Rith
authors.forename: Laurence
authors.initials: L
authors.lastname: Borand
authors.forename: Nima
authors.initials: N
authors.lastname: Asgari
authors.forename: Roger
authors.initials: R
authors.lastname: Frutos
authors.forename: Bertrand
authors.initials: B
authors.lastname: Guillard
authors.forename: Sok
authors.initials: S
authors.lastname: Touch
authors.forename: Vincent
authors.initials: V
authors.lastname: Deubel
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002160:Cambodia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012621:Seasons; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012367:RNA, Viral