title: Overexpression of NF-κB inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response.
abstract: IκBα is a member of the NF-κB inhibitor family that inhibits NF-κB activity by sequestering NF-κB in an inactive form in the cytosol. Unlike mammalian IκBα, which has been extensively studied, very little is known about the function of fish IκBα. In this study, we identified and analyzed an IκBα homologue, CsIκBα from half-smooth tongue sole (Cynoglossus semilaevis), a marine flatfish with important economic value. The deduced amino acid sequence of CsIκBα contains 308 residues and shares 58-82% overall sequence identities with the IκBα of a number of teleosts. In silico analysis identified in CsIκBα conserved domains that in mammals are known to be involved in phosphorylation, ubiquitination, and degradation of IκBα. Quantitative real time RT-PCR detected constitutive expression of CsIκBα in gut, spleen, liver, gill, heart, brain, muscle, and kidney. Experimental challenge with a bacterial pathogen-induced significant inductions of CsIκBα expression in head and trunk kidney, which, however, were transient and much lower in magnitude than that of interleukin-1β. To examine the effect of unregulated overexpression of CsIκBα in a live fish model, tongue sole were administered via intramuscular injection with plasmid pCNCsIkBa, which constitutively expresses CsIκBα. PCR, RT-PCR, and immunohistochemistry analysis showed that pCNCsIkBa was able to translocate to internal tissues, where transcription and translation of the recombinant CsIκBα took place. Compared to control fish, fish administered with pCNCsIkBa were impaired in the ability to block bacterial dissemination and survival in kidney and exhibited significantly reduced expression of multiple immune genes. These results suggest the possible existence in tongue sole of a NF-κB-IκBα signaling pathway that is negatively regulated by CsIκBα and required for effective defense against bacterial infection.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Xiao
authors.forename: Li
authors.initials: L
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005412:Flatfishes; D005822:Genetic Vectors; D060226:Head Kidney / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D054884:Host-Pathogen Interactions; D020672:I-kappa B Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007109:Immunity / Q000235:genetics; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation / Q000276:immunology; D014733:Vibrio / Q000276:immunology* / Q000472:pathogenicity; D014735:Vibrio Infections / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D020672:I-kappa B Proteins; D016328:NF-kappa B; D000072000:NF-KappaB Inhibitor alpha