title: Reduction of Escherichia coli on surfaces of utensils and development of a predictive model as a function of concentration and exposure time of chlorine.
abstract: Cross-contamination to fruit and vegetables can readily occur through contaminated surfaces; thus, there is a need to develop methods to inactivate microorganisms on the surfaces of various materials. The aim of this study was to develop methods to reduce the levels of Escherichia coli on the surfaces of various materials and to develop a predictive model as a function of chlorine concentration and exposure time. The reduction of E. coli on the surfaces of stainless steel, plastic, wood, rubber, glass, and ceramic at various chlorine concentrations (0-200 ppm) after a 0-5-min exposure was evaluated. The surface treatment at the maximum chlorine concentration (200 ppm) over a 5-min exposure reduced the E. coli contamination levels to 5.30, 5.18, 3.34, 4.69, 5.05, and 5.53 log CFU/cm(2) on the surfaces of stainless steel, plastic, wood, rubber, glass, and ceramic, respectively. Using these results, predictive models for the reduction of E. coli on surfaces of various materials using chlorine treatment were developed. Each model was significant (p<0.05) and defined as fit by the lack of fit and probability of normal residuals. It has measured the R(2) value to 0.9746. Therefore, the models presented in this study could be used to determine the minimum concentrations of chlorine and exposure times needed to control E. coli on the surfaces of various materials.
authors.affiliation: Department of Food Science and Technology, Chung-Ang University, Ansung, South Korea.
authors.forename: Seung-Bum
authors.initials: SB
authors.lastname: Baek
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Sang-Do
authors.initials: SD
authors.lastname: Ha
mesh_terms: D000704:Analysis of Variance; D002516:Ceramics; D002713:Chlorine / Q000494:pharmacology*; D015169:Colony Count, Microbial; D003297:Cooking and Eating Utensils / Q000592:standards*; D004202:Disinfectants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D005506:Food Contamination / Q000517:prevention & control*; D005898:Glass; D006801:Humans; D050296:Microbial Viability; D008954:Models, Biological; D010969:Plastics; D012408:Rubber; D013193:Stainless Steel; D013997:Time Factors; D014934:Wood
chemical_list: D004202:Disinfectants; D010969:Plastics; D013193:Stainless Steel; D002713:Chlorine; D012408:Rubber