title: Effect of ovarian aging on androgen biosynthesis in a cynomolgus macaque model.
abstract: OBJECTIVE
  The role of androgens in chronic disease pathogenesis, cognitive function and libido during menopause is of increasing interest. The aim of this study was to characterize the distribution and expression of androgenic proteins in the macaque ovary and to investigate the relationship between serum androgen concentrations, follicle number, and the persistence of androgenesis in the aging macaque ovary.
  
  
  METHODS
  The subjects were 26 adult female cynomolgus macaques. Ovaries were immunostained for cytochrome P450 17α-hydroxylase/17-20 lyase (P450c17), 3β-hydroxysteroid dehydrogenase (3βHSD), and cytochrome b5 (cytb5). Based on primordial follicle counts, animals were divided into tertiles (low (≤200), intermediate (226-1232), and high (2372-4356)) to evaluate differences in androgen staining and changes in serum androgen concentrations following ovariectomy.
  
  
  RESULTS
  Positive immunostaining for P450c17 and cytb5 within the theca interna layer of growing follicles persisted in advanced atretic follicles and secondary interstitial cells (residual stromal cells). Ovaries with low follicle numbers had less staining for all androgenic proteins compared to ovaries with higher numbers of growing follicles. Immunostaining for cytb5 was the most reliable marker for persistent androgenesis in ovaries with minimal primordial follicle numbers (<100) and residual stromal cells. Following ovariectomy, a significant decrease in testosterone (-27.7%, -30.8%, -27.5%; p < 0.01) and androstenedione (-33.4%, -35.7%, -46.0%; p < 0.01) was observed in monkeys with low, intermediate, and high primordial follicle counts, respectively.
  
  
  CONCLUSIONS
  Despite low follicle numbers, the aging macaque ovary retains the necessary proteins for androgenesis within residual stromal cells and contributes to peripheral androgen concentrations.
authors.affiliation: Department of Pathology/Comparative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC 27157-1040, USA.
authors.forename: K F
authors.initials: KF
authors.lastname: Ethun
authors.forename: C E
authors.initials: CE
authors.lastname: Wood
authors.forename: C R
authors.initials: CR
authors.lastname: Parker
authors.forename: J R
authors.initials: JR
authors.lastname: Kaplan
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: S E
authors.initials: SE
authors.lastname: Appt
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000728:Androgens / Q000096:biosynthesis* / Q000097:blood; D000818:Animals; D016922:Cellular Senescence; D004396:Coloring Agents / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008252:Macaca fascicularis; D023421:Models, Animal; D008991:Monitoring, Physiologic; D010052:Ovariectomy / Q000009:adverse effects; D011182:Postoperative Care / Q000379:methods; D011300:Preoperative Care / Q000379:methods; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism; D013799:Theca Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D000728:Androgens; D004396:Coloring Agents; D015786:Cytochromes b5; D015096:3-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase