title: Real-time expression of hTERT in primary melanoma biopsies.
abstract: Skin melanoma is by far the most lethal skin cancer, it is unpredictable by nature and presents a severe diagnostic problem. One of the major issues in melanoma diagnostics is to differentiate it with confidence from a dysplastic nevus. Thus, the aim of this study was to evaluate hTERT expression on a spectrum of dermal lesions (from normal skin toprimary melanoma) in order to examine its possible role as a diagnostic marker in melanoma diagnostics. In this study we analyzed the expression of hTERT by real-time PCR on 58 freshly obtained biopsy samples (4 samples of normal skin, 12 dermal nevi, 23 dysplastic nevi, 19 primary melanomas). Our results showed slightly greater hTERT expression in dysplastic nevi than melanomas with major data overlap. Considering the given results, hTERT does not seem to be a reliable diagnostic marker for melanoma.
authors.affiliation: University of Rijeka, School of Medicine, Department of Pathology, Rijeka, Croatia.
authors.forename: Miljenko
authors.initials: M
authors.lastname: Katunarić
authors.forename: Davor
authors.initials: D
authors.lastname: Jurisić
authors.forename: Ita
authors.initials: I
authors.lastname: Hadzisejdić
authors.forename: Ivan
authors.initials: I
authors.lastname: Kirin
authors.forename: Gordana
authors.initials: G
authors.lastname: Zamolo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D001706:Biopsy; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology; D019098:Telomerase / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C509186:TERT protein, human; D019098:Telomerase