title: CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids.
abstract: We report the design, synthesis, and characterization of a binary oligonucleotideprobe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.
authors.affiliation: Department of Chemistry, Columbia University, New York, NY 10027, USA.
authors.forename: Yiru
authors.initials: Y
authors.lastname: Peng
authors.forename: Chunmei
authors.initials: C
authors.lastname: Qiu
authors.forename: Steffen
authors.initials: S
authors.lastname: Jockusch
authors.forename: Amy M
authors.initials: AM
authors.lastname: Scott
authors.forename: Zengmin
authors.initials: Z
authors.lastname: Li
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000737:chemistry*; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D002232:Carbocyanines; D015345:Oligonucleotide Probes; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C085321:cyanine dye 5; D004247:DNA; C058667:cadmium selenide; C031238:zinc sulfide