title: [Simultaneous determination of 6 phenolic acids in coffee beans by reversed-phase high performance liquid chromatography].
abstract: A method of reversed-phase high performance liquid chromatography coupled with diode array detection (HPLC-DAD) was established for the simultaneous determination of 6 phenolic acids in green coffee beans. These phenolic acids are caffeic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid. The separation was achieved using a Kromasil C18 column (200 mm x 4.6 mm, 5 microm) under the gradient elution with the mobile phases of acetonitrile and 0.1% (v/v) formic acid/water. The 6 phenolic acids were well separated within 45 min. The recoveries were from 90.76% to 104.73% with the relative standard deviations between 0.7% and 3.9%. The method is simple, rapid and highly sensitive, suitable for the simultaneous determination of 6 phenolic acids and quality control of coffee beans.
authors.affiliation: National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha 410128, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Long
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Yuan
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Li
authors.forename: Zhonghu
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002109:Caffeic Acids / Q000032:analysis*; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D003069:Coffee / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000032:analysis*; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D002109:Caffeic Acids; D002726:Chlorogenic Acid; D003069:Coffee; D062385:Hydroxybenzoates; D011801:Quinic Acid; C017616:phenolic acid; C473200:5-caffeoylquinic acid; C040048:caffeic acid