title: Capsicum annuum L. methanolic extract inhibits ovalbumin-induced airway inflammation and oxidative stress in a mouse model of asthma.
abstract: The pepper fruit of Capsicum annuum L. is used as a food, spice, and topical medicine. Here, we investigated the effect of a methanolic C. annuum L. extract (CAE) in a mouse model of ovalbumin-induced allergic airway inflammation. Animals were treated with CAE by oral gavage before ovalbumin challenge. After ovalbumin challenge, airway responsiveness to methacholine, influx of inflammatory cells into the lung, cytokine levels in bronchoalveolar lavage fluid and lung, nuclear factor-κB (NF-κB) activity in lungs, and lung histopathology were assessed. Oral treatment with CAE significantly reduced the pathophysiological signs of allergic airway disease, including increased inflammatory cell recruitment to the airways, airway hyperresponsiveness, and increased levels of T-helper type 2 cytokines. Reactive oxygen species were also decreased in cells from bronchoalveolar lavage fluid. In addition, we found that administration of CAE attenuated ovalbumin-induced increases in NF-κB activity in lungs. Collectively, these results suggest that CAE may be an effective oral treatment for allergic airway inflammation by virtue of its antioxidant activity.
authors.affiliation: Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon-gun, Chungbuk, Korea.
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Jang
authors.forename: Se-Mi
authors.initials: SM
authors.lastname: Kim
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Yuk
authors.forename: Ok-Kyoung
authors.initials: OK
authors.lastname: Kwon
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Hyeok
authors.initials: H
authors.lastname: Jeong
authors.forename: Kyung-Seop
authors.initials: KS
authors.lastname: Ahn
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001249:Asthma / Q000188:drug therapy* / Q000473:pathology; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002212:Capsicum / Q000737:chemistry*; D016207:Cytokines / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005638:Fruit / Q000737:chemistry; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D008168:Lung / Q000187:drug effects / Q000473:pathology; D016210:Methacholine Chloride / Q000009:adverse effects; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D010047:Ovalbumin / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D016328:NF-kappa B; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D016210:Methacholine Chloride; D007073:Immunoglobulin E; D010047:Ovalbumin