title: Characterization of human melanoma cell lines and melanocytes by proteome analysis.
abstract: We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography - tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were over-expressed in both melanoma cell lines as compared to melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, protein DJ-1, cyclophilin A and cofilin-1. We show, for the first time, that only specific isoforms of these molecules are over-expressed in melanoma. Different protein profiles were also found between each individual melanoma cell line and the melanocytes. s-Methyl-5-thioadenosine phosphorylase, ubiquitin and ribosomal protein S27 a precursor, the basic form of protein DJ-1, annexin a1, proliferation associated protein 2g4, isoform alfa-enolase of alfa-enolase, protein disulfide-isomerase precursor, and elongation factor 2 were more strongly expressed in A375 cells compared to melanocytes. In 526 cells, 60s acidic ribosomal protein p1 and calreticulin precursor were more highly expressed than in melanocytes. These molecular differences may help in better understanding melanoma development and its different responsiveness to therapies. The identified proteins could be exploited as biomarkers or therapeutic targets for melanoma.
authors.affiliation: Institute of Genetics and Biophysics (IGB) A.Buzzati-Traverso, Consiglio Nazionale delle Ricerche (CNR), Naples, Italy. caputo@igb.cnr.it
authors.forename: Emilia
authors.initials: E
authors.lastname: Caputo
authors.forename: Luigi
authors.initials: L
authors.lastname: Maiorana
authors.forename: Valeria
authors.initials: V
authors.lastname: Vasta
authors.forename: Franca M
authors.initials: FM
authors.lastname: Pezzino
authors.forename: Sudhir
authors.initials: S
authors.lastname: Sunkara
authors.forename: Kieran
authors.initials: K
authors.lastname: Wynne
authors.forename: Giuliano
authors.initials: G
authors.lastname: Elia
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Marincola
authors.forename: James A
authors.initials: JA
authors.lastname: McCubrey
authors.forename: Massimo
authors.initials: M
authors.lastname: Libra
authors.forename: Salvatore
authors.initials: S
authors.lastname: Travali
authors.forename: Marian
authors.initials: M
authors.lastname: Kane
mesh_terms: D015415:Biomarkers; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D051338:Cofilin 1 / Q000378:metabolism; D021984:Cyclophilin A / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D037483:Galectin 1 / Q000378:metabolism; D006801:Humans; D007168:IMP Dehydrogenase / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D008544:Melanocytes / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism; D000071617:Protein Deglycase DJ-1; D020033:Protein Isoforms / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D015415:Biomarkers; C495810:CFL1 protein, human; D051338:Cofilin 1; D037483:Galectin 1; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D020033:Protein Isoforms; D011498:Protein Precursors; D020543:Proteome; D007168:IMP Dehydrogenase; C501687:IMPDH2 protein, human; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D021984:Cyclophilin A