title: Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China.
abstract: The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequence identity in all gene products except the coat protein. Based on the molecular criterion (>10% amino acid sequence difference) for species demarcation in the genus Polerovirus, the virus represents a distinct species for which the name Brassica yellows virus (BrYV) is proposed. Interestingly, there were two genotypes of BrYV, which mainly differed in the 5'-terminal half of the genome.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, People's Republic of China. xhy236@yahoo.com.cn
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Xiang
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Dong
authors.forename: Qiao-Xia
authors.initials: QX
authors.lastname: Shang
authors.forename: Cui-Ji
authors.initials: CJ
authors.lastname: Zhou
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.forename: Jia-Lin
authors.initials: JL
authors.lastname: Yu
authors.forename: Cheng-Gui
authors.initials: CG
authors.lastname: Han
mesh_terms: D001483:Base Sequence; D001937:Brassica / Q000821:virology*; D002681:China; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D005838:Genotype; D052679:Luteoviridae / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral