title: Impact of genetic variations of the CYP1A1, GSTT1, and GSTM1 genes on the risk of coronary artery disease.
abstract: Carcinogenic and toxic molecules produce DNA adducts that contribute to the development of atherosclerosis. Genetic polymorphisms of xenobiotic-detoxified enzymes, which control the level of DNA adducts, may affect both enzymatic activity and individual susceptibility to coronary artery disease (CAD). In this study we investigated the effects of genetic polymorphisms of the CYP1A1*2C, GSTT1, and GSTM1 enzymes on CAD risk in a Turkish population. Genotypes were determined for 132 CAD patients and 151 healthy controls by the polymerase chain reaction/restriction fragment length polymorphism method. There were no significant differences between patients and controls in terms of CYP1A1, GSTT1, and GSTM1 genotypes. Analysis of the possible interactions between the genotypes, after adjustment for the risk factors, demonstrated that individuals carrying CYP1A1 variant GSTT1 null genotypes had an 8.907-fold increased CAD risk compared to their wild status (p<0.05). We suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in CAD. Therefore, CYP1A1 and GSTM1 polymorphisms should be considered as important parameters for the prediction of CAD.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Ankara University, Ankara, Turkey. mtaspinartr@yahoo.com
authors.forename: Mehmet
authors.initials: M
authors.lastname: Taspinar
authors.forename: Sena
authors.initials: S
authors.lastname: Aydos
authors.forename: Onur
authors.initials: O
authors.lastname: Sakiragaoglu
authors.forename: Irfan Veysel
authors.initials: IV
authors.lastname: Duzen
authors.forename: Adnan
authors.initials: A
authors.lastname: Yalcinkaya
authors.forename: Derya
authors.initials: D
authors.lastname: Oztuna
authors.forename: Hasmet
authors.initials: H
authors.lastname: Bardakci
authors.forename: Eralp
authors.initials: E
authors.lastname: Tutar
authors.forename: Asuman
authors.initials: A
authors.lastname: Sunguroglu
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000502:physiology; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation / Q000502:physiology; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012306:Risk
chemical_list: D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; C569463:CYP1A1 protein, human; C413545:glutathione S-transferase T1