title: Antioxidant and anti-inflammatory activities of quercetin 7-O-β-D-glucopyranoside from the leaves of Brasenia schreberi.
abstract: Brasenia schreberi Gmel. (Cabombaceae) is an aquatic plant that grows in eastern Asia, Australia, Africa, and North and Central America. B. schreberi leaf extracts were obtained by sequential solvent extraction with dichloromethane, methanol, and water. The antioxidant potential of each extract was assessed by using the oxygen radical absorbance capacity (ORAC) assay. With this method, methanol and water extracts were found to be active with mean ± standard deviation values of 7 ± 2 and 5.1 ± 0.5 μmol Trolox® equivalents (TE)/mg, respectively. Two major phenolic compounds, quercetin-7-O-β-D-glucopyranoside and gallic acid, were respectively isolated from the methanolic and water extracts. Both compounds exhibited antioxidant activities, in particular quercetin-7-O-β-D-glucopyranoside (ORAC value, 18 ± 4  μmol TE/μmol). In contrast to its well-known antioxidant homologue quercetin, quercetin-7-O-β-D-glucopyranoside does not inhibit growth of human fibroblasts (WS-1) or murine macrophages (RAW 264.7). Some flavonoids have been reported to possess beneficial effects in cardiovascular and chronic inflammatory diseases associated with overproduction of nitric oxide. Quercetin-7-O-β-D-glucopyranoside possesses anti-inflammatory activity, inhibiting expression of inducible nitric oxide synthase and release of nitric oxide by lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. Quercetin-7-O-β-D-glucopyranoside also inhibited overexpression of cyclooxygenase-2 and granulocyte macrophage-colony-stimulating factor.
authors.affiliation: Laboratory for Analysis and Separation of Plant Species (LASEVE), Université du Québec à Chicoutimi, Chicoutimi, Québec, Canada. jean_legault@uqac.ca
authors.forename: Jean
authors.initials: J
authors.lastname: Legault
authors.forename: Tommy
authors.initials: T
authors.lastname: Perron
authors.forename: Vakhtang
authors.initials: V
authors.lastname: Mshvildadze
authors.forename: Karl
authors.initials: K
authors.lastname: Girard-Lalancette
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Perron
authors.forename: Catherine
authors.initials: C
authors.lastname: Laprise
authors.forename: Pascal
authors.initials: P
authors.lastname: Sirois
authors.forename: André
authors.initials: A
authors.lastname: Pichette
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D015153:Blotting, Western; D002460:Cell Line; D002855:Chromatography, Thin Layer; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005960:Glucosides; D008070:Lipopolysaccharides; D010936:Plant Extracts; C110311:quercetin 7-O-glucopyranoside; D009569:Nitric Oxide; D005707:Gallic Acid; D011794:Quercetin; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2