title: Isolation of mesenchymal stem cells from the mandibular marrow aspirates.
abstract: OBJECTIVE
  The aim of this study was to determine if mesenchymal stem cells (MSCs) could be obtained from mandible marrow aspirates.
  
  
  METHODS
  In 5 patients, 10 mL marrow aspirates were obtained from both the mandible and the iliac crest. Second passage MSCs were characterized by fluorescence-activated cell sorting (FACS) analysis using MSC-specific cell surface marker, and fifth-passage cells were differentiated into 3 mesenchymal tissues in vitro.
  
  
  RESULTS
  Average total cell yields of MSCs were 2.8 ± 1.8 × 10(5) per 10 mL marrow aspirates from the mandible. Immunophenotypes of MSCs isolated from the mandible and iliac crest were highly similar, as indicated by FACS analysis. Differentiation into mesodermal cell types, such as osteocytes, chondrocytes, and adipocytes, was successfully achieved in all MSC isolates from all aspirates.
  
  
  CONCLUSIONS
  The MSCs can be isolated from the mandibular aspirates, providing an alternative accessible source of MSCs for the treatment of future dental and craniomaxillofacial diseases.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Asan Medical Center, College of Medicine, Ulsan University, Seoul, Korea. bukyu.lee@amc.seoul.kr
authors.forename: Bu-Kyu
authors.initials: BK
authors.lastname: Lee
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: David
authors.initials: D
authors.lastname: Mack
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Oh
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D001707:Biopsy, Needle; D001854:Bone Marrow Cells / Q000166:cytology*; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D019902:Chondrocytes / Q000166:cytology; D003470:Culture Media; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007085:Ilium / Q000166:cytology; D016130:Immunophenotyping; D008297:Male; D008334:Mandible / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology; D055815:Young Adult
chemical_list: D003470:Culture Media