title: Gamma-radiation induced modifications in substrate specificity of glucose dehydrogenase and carbon source utilization pattern of phosphate-solubilizing Pantoea strains.
abstract: Glucose, maltose, and mannose as sole carbon sources, induced synthesis of glucose dehydrogenase (GDH) in three strains of Pantoea with specific activities from 0.14 to 0.6 U/mg proteins. Utilization of lactose indicated that the enzyme belongs to GDH type B isozyme. Of mutant clones, developed through radiation mutagenesis, P2-M2 utilized ribose with GDH specific activity of 0.57 U/mg protein, P4-M3 grown on glucose gave 1.5 U/mg protein and P4-M5 had high activities, when grown on galactose, maltose, and lactose. Clones P3-M2 and P2-M5 had versatile utilization of sugars and released higher amounts of P from tri-calcium phosphate and can be efficiently used for biofertilization.
authors.affiliation: Radiation Application Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
authors.forename: Young-Keun
authors.initials: YK
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Senthilkumar
authors.forename: Il Yun
authors.initials: IY
authors.lastname: Jeong
authors.forename: K
authors.initials: K
authors.lastname: Annapurna
authors.forename: K
authors.initials: K
authors.lastname: Swarnalakshmi
mesh_terms: D050260:Carbohydrate Metabolism / Q000502:physiology* / Q000528:radiation effects; D002244:Carbon / Q000378:metabolism; D005720:Gamma Rays; D024861:Genetic Enhancement / Q000379:methods*; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009154:Mutation / Q000528:radiation effects; D020636:Pantoea / Q000145:classification / Q000201:enzymology* / Q000528:radiation effects*; D010710:Phosphates / Q000378:metabolism*; D011829:Radiation Dosage; D012995:Solubility; D013045:Species Specificity; D013379:Substrate Specificity / Q000528:radiation effects
chemical_list: D010710:Phosphates; D008320:Maltose; D002244:Carbon; D042843:Glucose 1-Dehydrogenase; D005947:Glucose; D008358:Mannose