title: Detection of bat coronaviruses from Miniopterus fuliginosus in Japan.
abstract: Bats have great potential as reservoirs for emerging viruses such as severe acute respiratory syndrome-coronavirus. In this study, bat coronaviruses (BtCoVs) were detected by RT-PCR from intestinal and fecal specimens of Miniopterus fuliginosus breeding colonies in Wakayama Prefecture caves, where we previously identified bat betaherpesvirus 2. Two primer sets were used for the detection of BtCoV: one was for the RNA-dependent RNA polymerase (RdRp) region and the other was for the spike (S) protein region. Eleven and 73% of intestinal and fecal specimens, respectively, were positive for RdRp region, and 2 and 40% of those were positive for S protein region. Sequencing and phylogenetic analysis showed that the detected BtCoV belonged to the group 1 (alpha) coronaviruses. These data suggest that BtCoV is endemic in M. fuliginosus in Japan.
authors.affiliation: Department of Virology 3, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Shirato
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Shumpei
authors.initials: S
authors.lastname: Tsuda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Suzuki
authors.forename: Shumpei
authors.initials: S
authors.lastname: Watanabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimoda
authors.forename: Naoya
authors.initials: N
authors.lastname: Ueda
authors.forename: Koichiro
authors.initials: K
authors.lastname: Iha
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kyuwa
authors.forename: Daiji
authors.initials: D
authors.lastname: Endoh
authors.forename: Shutoku
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kurane
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Hiroomi
authors.initials: H
authors.lastname: Akashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
mesh_terms: D000818:Animals; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D005243:Feces / Q000821:virology; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins