title: NADPH oxidase p22phox polymorphisms and oxidative stress in patients with obstructive sleep apnoea.
abstract: BACKGROUND
  Obstructive Sleep Apnoea (OSA) is associated with increased oxidative stress. NADPH oxidases are the main source of Reactive Oxygen Species (ROS) in the vasculature. Several polymorphisms related to NADPH oxidase expression or activity have been identified. We compared the distribution of the allelic frequencies of A-930G and C242T polymorphisms and their possible relationship with the levels of 8-isoprostanes as a marker of oxidative stress in patients with OSA and in a control group without OSA.
  
  
  METHODS
  This is a case-control study. We determined the A-930G and C242T p22phox genotypes in 427 patients with OSA and in 139 healthy subjects recruited from the Sleep Unit of Son Dureta University Hospital, (Palma de Mallorca, Spain). 8-Isoprostane was measured as an oxidative stress marker.
  
  
  RESULTS
  The distribution of the p22phox genotypes in OSA and in control subjects was different. The risk of OSA was associated with the presence of the G allele in the A-930G p22phox independently of age, gender, Body Mass Index (BMI), hypertension, dyslipemia and diabetes, but no association was found with the C242T polymorphism. The median level of 8-isoprostane was significantly higher in OSA patients. Synergic effect in 8-Isoprostane levels was observed when these two polymorphisms were analysed together.
  
  
  CONCLUSIONS
  the A-930G polymorphism of the p22phox gene may play an important role in genetic susceptibility to OSA. Furthermore, the C242T and A-930G polymorphisms of the p22phox gene have a synergic effect on the 8-isoprostane levels, suggesting that they may be involved in the development of oxidative stress in these patients.
authors.affiliation: Unidad de Investigación, Hospital Universitario Son Espases, Ctra Valldemossa 79, 07120 Palma de Mallorca, Spain. javier.pierola@ssib.es
authors.forename: Javier
authors.initials: J
authors.lastname: Piérola
authors.forename: Alexandra
authors.initials: A
authors.lastname: Alemany
authors.forename: Aina
authors.initials: A
authors.lastname: Yañez
authors.forename: Mónica
authors.initials: M
authors.lastname: de-la-Peña
authors.forename: Manuel
authors.initials: M
authors.lastname: Sánchez-de-la-Torre
authors.forename: Cristina
authors.initials: C
authors.lastname: Esquinas
authors.forename: Camino
authors.initials: C
authors.lastname: Pérez-Gutierrez
authors.forename: Bartolomé
authors.initials: B
authors.lastname: Burguera
authors.forename: Ferran
authors.initials: F
authors.lastname: Barbé
authors.forename: Antonia
authors.initials: A
authors.lastname: Barceló
mesh_terms: D015992:Body Mass Index; D016022:Case-Control Studies; D005260:Female; D005784:Gene Amplification; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D020181:Sleep Apnea, Obstructive / Q000235:genetics* / Q000503:physiopathology
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human