title: A colorimetric and ratiometric fluorescent probe for quantification of bovine serum albumin.
abstract: A 4-aminonaphthalimide-based ratiometric fluorescent probe 1 employing the internal charge transfer (ICT) mechanism was designed and synthesized to detect bovine serum albumin (BSA). The interaction of 1 and BSA was investigated by fluorescence and UV-vis absorption spectroscopy. Upon treatment with BSA, the probe successfully exhibited a ratiometric fluorescent response at 540 nm and 480 nm. The fluorescent intensity ratio at 540 nm and 480 nm (F(540)/F(480)) increases linearly with BSA concentration in the range of 0-75.0 μg mL(-1) and the detection limit was about 2.4 ng mL(-1). Our strategy is expected to provide a methodology to quantify BSA either by a normal or by a ratiometric and colorimetric way with high sensitivity.
authors.affiliation: Key Laboratory of Cluster Science of Ministry of Education, Department of Chemistry, School of Science, Beijing Institute of Technology, Beijing, 100081, People's Republic of China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Zeng
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Zhang
authors.forename: Baocun
authors.initials: B
authors.lastname: Zhu
authors.forename: Hongying
authors.initials: H
authors.lastname: Jia
authors.forename: Yamin
authors.initials: Y
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Xue
mesh_terms: D000818:Animals; D002417:Cattle; D003124:Colorimetry; D005456:Fluorescent Dyes / Q000737:chemistry*; D010797:Phthalimides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D010797:Phthalimides; D012710:Serum Albumin, Bovine