title: Anti-inflammatory principles from Cordyceps sinensis.
abstract: In order to explore the anti-inflammatory principles of the mycelia of Cordyceps sinensis, the crude extract and partially purified fractions were examined for their inhibition of superoxide anion generation and elastase release. Further chemical investigation of the bioactive fractions has resulted in the identification of 50 compounds, including five constituents, cordysinins A-E (1-5), reported from a natural source for the first time. In addition, compounds were examined for their anti-inflammatory activity. 1-(5-Hydroxymethyl-2-furyl)-β-carboline displayed the most significant inhibition of superoxide anion generation and elastase release with IC50 values of 0.45±0.15 and 1.68±0.32 μM, respectively.
authors.affiliation: Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan, Republic of China.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Yang
authors.forename: Ping-Chung
authors.initials: PC
authors.lastname: Kuo
authors.forename: Tsong-Long
authors.initials: TL
authors.lastname: Hwang
authors.forename: Tian-Shung
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology; D002243:Carbolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D032661:Cordyceps / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D025282:Mycelium / Q000737:chemistry; D010196:Pancreatic Elastase / Q000032:analysis / Q000097:blood / Q000378:metabolism; D010851:Picrates / Q000494:pharmacology; D013237:Stereoisomerism; D013481:Superoxides / Q000037:antagonists & inhibitors; D013624:Taiwan
chemical_list: D000893:Anti-Inflammatory Agents; D001713:Biphenyl Compounds; D002243:Carbolines; D010851:Picrates; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D010196:Pancreatic Elastase