title: Modelling real-time simultaneous saccharification and fermentation of lignocellulosic biomass and organic acid accumulation using dielectric spectroscopy.
abstract: Dielectric spectroscopy (DS) is routinely used in yeast and mammalian fermentations to quantitatively monitor viable biomass through the inherent capacitance of live cells; however, the use of DS to monitor the enzymatic break down of lignocellulosic biomass has not been reported. The aim of the current study was to examine the application of DS in monitoring the enzymatic saccharification of high sugar perennial ryegrass (HS-PRG) fibre and to relate the data to changes in chemical composition. DS was capable of both monitoring the on-line decrease in PRG fibre capacitance (C=580 kHz) during enzymatic hydrolysis, together with the subsequent increase in conductivity (G=580 kHz) resulting from the production of organic acids during microbial growth. Analysis of the fibre fractions revealed >50% of HS-PRG lignocellulose had undergone enzymatic hydrolysis. These data demonstrated the utility of DS biomass probes for on-line monitoring of simultaneous saccharification and fermentation (SSF).
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK.
authors.forename: David N
authors.initials: DN
authors.lastname: Bryant
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Morris
authors.forename: David
authors.initials: D
authors.lastname: Leemans
authors.forename: Steven A
authors.initials: SA
authors.lastname: Fish
authors.forename: Stephen
authors.initials: S
authors.lastname: Taylor
authors.forename: John
authors.initials: J
authors.lastname: Carvell
authors.forename: Robert W
authors.initials: RW
authors.lastname: Todd
authors.forename: Daniel
authors.initials: D
authors.lastname: Logan
authors.forename: Matthew
authors.initials: M
authors.lastname: Lee
authors.forename: Naroa
authors.initials: N
authors.lastname: Garcia
authors.forename: Andrew
authors.initials: A
authors.lastname: Ellis
authors.forename: Joe A
authors.initials: JA
authors.lastname: Gallagher
mesh_terms: D018533:Biomass; D002138:Calibration; D002241:Carbohydrates / Q000737:chemistry*; D005285:Fermentation; D008031:Lignin / Q000378:metabolism*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D008031:Lignin