title: Identification and interaction of amino acids with leucine-anthracene reagent by TLC and spectrophotometry: experimental and theoretical studies.
abstract: A new reagent has been synthesized by coupling anthracene moiety to L-leucine. The reagent is characterized by different analytical techniques. It is capable for easy identification of various amino acids on thin-layer chromatography plates by developing distinguishable colors (detection limit between 0.1-0.5 μg at cold condition and 0.1-0.4 μg after heating). This reagent also binds with different amino acids very strongly in solution (methanol). Estimation of equilibrium binding constants of this new reagent with different amino acids has also been carried out. The values of the binding constants are lowest for L-Tyrosine (6.86 × 10³ dm³ mol(-1)) and highest for L-Arginine monohydrochloride (8.86 × 10⁵ dm³ mol(-1)) at 25°C. A theoretical study (Hartree-Fock) has been performed to investigate the interaction of the reagent with a representative amino acid, glycine.
authors.affiliation: Department of Chemistry, The University of Burdwan, Burdwan 713104, India.
authors.forename: Animesh
authors.initials: A
authors.lastname: Sahana
authors.forename: Sudipta
authors.initials: S
authors.lastname: Das
authors.forename: Raja
authors.initials: R
authors.lastname: Saha
authors.forename: Moumita
authors.initials: M
authors.lastname: Gupta
authors.forename: Subrata
authors.initials: S
authors.lastname: Laskar
authors.forename: Debasis
authors.initials: D
authors.lastname: Das
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000873:Anthracenes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D002855:Chromatography, Thin Layer / Q000379:methods*; D007930:Leucine / Q000031:analogs & derivatives* / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular; D010940:Plant Proteins / Q000032:analysis; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012996:Solutions
chemical_list: D000596:Amino Acids; D000873:Anthracenes; D010940:Plant Proteins; D012996:Solutions; D007930:Leucine; D000432:Methanol