title: [Effect of lysyl oxidase on migration and adhesion of human gastric cancer HGC-27 cells in vitro].
abstract: OBJECTIVE
  To study the effects of lysyl oxidase (LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro.
  
  
  METHODS
  The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments.
  
  
  RESULTS
  Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) × 10(3)/ml to (7.78 ± 0.11) × 10(3)/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98 ± 0.15) × 10(3)/ml to (8.35 ± 0.10) × 10(3)/ml, both < 0.05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8.02 ± 0.11) × 10(3)/ml and the number of cells with heterogeneous adhesion was down to (7.93 ± 0.07) × 10(3)/ml (P < 0.05).
  
  
  CONCLUSIONS
  LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.
authors.affiliation: Department of Pathogenic Biology and Immunology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Bai-jing
authors.initials: BJ
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Huang
authors.forename: Mei
authors.initials: M
authors.lastname: Han
authors.forename: Yu-qiao
authors.initials: YQ
authors.lastname: Wang
authors.forename: Jian-ning
authors.initials: JN
authors.lastname: Zhao
authors.forename: Bo-jian
authors.initials: BJ
authors.lastname: Sun
authors.forename: Jia-jing
authors.initials: JJ
authors.lastname: Lin
authors.forename: Ting-ge
authors.initials: TG
authors.lastname: Song
mesh_terms: D000629:Aminopropionitrile / Q000008:administration & dosage / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009361:Neoplasm Invasiveness; D008249:Protein-Lysine 6-Oxidase / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D013274:Stomach Neoplasms / Q000201:enzymology / Q000473:pathology*
chemical_list: D000629:Aminopropionitrile; D008249:Protein-Lysine 6-Oxidase