title: Variation of telomeric DNA content in gingiva and dental pulp.
abstract: OBJECTIVE
  The purpose of the present study was to examine the age- and tissue-related variations of the telomere length in gingiva and dental pulp of donor patients.
  
  
  METHODS
  We quantified the relative telomeric DNA content corresponding to the telomere length in gingiva or dental pulp from donor patients (male and female, aged at 19-68) by using genomic DNA of oral tissues in dot-blot hybridization with telomere-specific probe.
  
  
  RESULTS
  Telomeric DNA content in the dental pulp showed a negative correlation with the age of donor patients, with smaller telomeric DNA content observed in the elders (p<0.05). In age-matched gingival samples, the average telomeric DNA content was not significantly different between male and female donors. In the age- and gender-matched samples, telomeric DNA content was significantly greater (p<0.001) in dental pulp than in gingiva.
  
  
  CONCLUSIONS
  The telomere length is greater in the dental pulp than in the gingiva. In the dental pulp, but not in the gingiva, telomere length shortens with age.
authors.affiliation: Graduate School of Tokyo Medical and Dental University, Tokyo 113-8549, Japan. kumei.bch@tmd.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kumei
authors.forename: Hideo
authors.initials: H
authors.lastname: Akiyama
authors.forename: Takuo
authors.initials: T
authors.lastname: Onizuka
authors.forename: Chihiro
authors.initials: C
authors.lastname: Kobayashi
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D004247:DNA / Q000378:metabolism*; D003782:Dental Pulp / Q000378:metabolism*; D005260:Female; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D016615:Telomere / Q000378:metabolism*
chemical_list: D004247:DNA