title: Genome-wide measurement of histone H3 replacement dynamics in yeast.
abstract: Chromatin plays critical roles in processes governed in different timescales - responses to environmental changes require rapid plasticity, while long-term stability through multiple cell generations requires epigenetically heritable chromatin. Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis. Here, we describe a protocol for measuring histone replacement rates genome wide in the budding yeast Saccharomyces cerevisiae. With suitable modifications, this protocol could be applied to other organisms, or to replacement dynamics of other DNA-associated proteins.
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, USA. Oliver.Rando@umassmed.edu
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Rando
mesh_terms: D000818:Animals; D002417:Cattle; D047369:Chromatin Immunoprecipitation; D046508:Culture Techniques; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D016681:Genome, Fungal / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D007422:Intestines / Q000201:enzymology; D008836:Micrococcal Nuclease / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D006657:Histones; D009707:Nucleosomes; D016372:RNA, Antisense; D010744:Phosphoric Monoester Hydrolases; D008836:Micrococcal Nuclease