title: Coproantigen capture ELISA for detection of Clonorchis sinensis infection in experimentally infected rats.
abstract: The present study investigated the diagnostic value of an ELISA for the detection of Clonorchis sinensis antigen in the feces of experimentally infected rats. A mouse polyclonal IgG antibody against adult C. sinensis crude antigen (CsAg) was used to capture the C. sinensis coproantigen. The detection limit for pure CsAg was 20 ng/ml in sample buffer and 40 ng/ml in uninfected fecal extract. The test was evaluated using a follow-up of five groups of rats experimentally infected with 100, 50, 10, 5 and 1 metacercariae of C. sinensis and an uninfected control group. Coproantigen was detected in all infected groups of rats from 2 weeks of infection, whereas fecal eggs were not observed until 3 weeks of infection. As the infection period progressed, the fecal CsAg concentration increased in all groups of infected rats, even those infected with a single metacercaria. The fecal CsAg concentration was correlated positively with fecal egg counts and worm burden. This coproantigen capture ELISA is highly sensitive for the detection of CsAg in rat feces, and with further development, should be useful for mass screening of human subjects in clonorchiasis-endemic areas.
authors.affiliation: Department of Parasitology and Tropical Medicine, Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110–799, Republic of Korea.
authors.forename: S M
authors.initials: SM
authors.lastname: Mazidur Rahman
authors.forename: Min-Ho
authors.initials: MH
authors.lastname: Choi
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Bae
authors.forename: Sung-Tae
authors.initials: ST
authors.lastname: Hong
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology; D002681:China; D003003:Clonorchiasis / Q000175:diagnosis* / Q000276:immunology; D003004:Clonorchis sinensis / Q000254:growth & development / Q000276:immunology*; D003530:Cyprinidae / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000469:parasitology*; D008297:Male; D058488:Metacercariae / Q000254:growth & development / Q000276:immunology; D023421:Models, Animal; D010270:Parasite Egg Count; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth