title: Involvement of ecto-5'-nucleotidase/CD73 in U138MG glioma cell adhesion.
abstract: Glioblastoma multiform is the most common and aggressive type of brain tumor. The overexpression of ecto-5'-nucleotidase/CD73 (ecto-5'-NT/CD73), an adhesion molecule and the main enzymatic source of extracellular adenosine, has been reported in tumor cells, and it is emerging as a component of glioma progression. Here, we evaluated the involvement of ecto-5'-NT/CD73 in cell adhesion through its interaction with different components of the extracellular matrix in the human U138MG glioma cell line. The results indicated that adenosine induced an increase in glioma cell adhesion. The treatment of glioma cells with adenosine receptor antagonists, APCP (α,β-methylene ADP) and dipyridamole prevented the adenosine effect, indicating the participation of extracellular and intracellular signaling pathways in cell adhesion mediated by adenosine. The ECM protein laminin (lam) and chondroitin sulfate (ChS) modulated the ecto-5'-NT/CD73 activity and glioma adhesion in a parallel manner, suggesting the involvement of purinergic signaling in the effects mediated by the extracellular matrix. Taken together, these results suggest that ecto-5'-NT/CD73, an important producer of extracellular adenosine, may modulate glioma cell adhesion and tumor cell-extracellular matrix interactions.
authors.affiliation: Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-anexo, Porto Alegre, RS, 90035-003, Brazil.
authors.forename: Angélica Regina
authors.initials: AR
authors.lastname: Cappellari
authors.forename: Gabriela Jouglard
authors.initials: GJ
authors.lastname: Vasques
authors.forename: Luci
authors.initials: L
authors.lastname: Bavaresco
authors.forename: Elizandra
authors.initials: E
authors.lastname: Braganhol
authors.forename: Ana Maria Oliveira
authors.initials: AM
authors.lastname: Battastini
mesh_terms: D015720:5'-Nucleotidase / Q000502:physiology*; D000241:Adenosine / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D005109:Extracellular Matrix / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000502:physiology; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D058915:Purinergic P1 Receptor Antagonists; D011687:Purines
chemical_list: D058851:GPI-Linked Proteins; D058915:Purinergic P1 Receptor Antagonists; D011687:Purines; D015720:5'-Nucleotidase; C548241:NT5E protein, human; D000241:Adenosine