title: An efficient method for recombineering GAL4 and QF drivers.
abstract: Neural circuit mapping and manipulation are facilitated by independent control of gene expression in pre- and post-synaptic neurons. The GAL4/UAS and Q binary transcription systems have the potential to provide this capability. Of particular use in neural circuit mapping would be GAL4 and QF drivers specific for neurotransmitters and neurotransmitter receptors. Recently available Drosophila genomic BAC libraries make recombineering large genes including those specific for neurotransmitters and neurotransmitter receptors feasible. Here the functionality of cassettes that allow efficient recombineering of GAL4 and QF drivers based on kanamycin selection is demonstrated in Drosophila. The cassettes should, however, be generalizable for recombineering in other species.
authors.affiliation: Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA. sstowers@montana.edu
authors.forename: R Steven
authors.initials: RS
authors.lastname: Stowers
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D016254:Mutagenesis, Insertional / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors