title: Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination.
abstract: In India, the proportion of bovines vaccinated against foot-and-mouth disease (FMD) is increasing since the implementation of the Government supported 'FMD Control Programme', and non-structural protein (NSP)-based serological assays for discriminating between antibodies induced by infection or vaccination (DIVA) could be useful. The FMD virus NSP 3AB was expressed in a prokaryotic system and an indirect ELISA (r3AB(3) I-ELISA) was developed and validated as a screening assay for detecting virus in vaccinated bovines. The diagnostic sensitivity of the assay was estimated to be 96%, while the diagnostic specificity varied between the naïve and vaccinates as 99.1% and 96.4%, respectively. This assay could detect antibodies to 3AB (3AB-Ab) from 10 to as late as 900 days post-infection in cattle infected experimentally. The "in-house" assay demonstrated higher sensitivity than a commercial 3ABC ELISA kit particularly with samples obtained from the late stages of infection. Transient post-vaccinal 3AB-Ab response could be detected in one of the three commercial vaccines during the six-month vaccination regimen, which emphasizes the fact that for a DIVA-compatible diagnostic strategy to be a realistic option, all vaccines need to be quality checked for the NSP content.
authors.affiliation: Project Directorate on Foot and Mouth Disease, Indian Veterinary Research Institute Campus, Mukteswar, Nainital 263138, Uttarakhand, India.
authors.forename: Jajati K
authors.initials: JK
authors.lastname: Mohapatra
authors.forename: Laxmi K
authors.initials: LK
authors.lastname: Pandey
authors.forename: Aniket
authors.initials: A
authors.lastname: Sanyal
authors.forename: Bramhadev
authors.initials: B
authors.lastname: Pattnaik
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D008297:Male; D055550:Protein Stability; D011933:Reagent Kits, Diagnostic / Q000662:veterinary; D011994:Recombinant Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: C109754:3ABC protein, virus; D000914:Antibodies, Viral; D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014765:Viral Vaccines