title: A genome-wide microarray highlights the antiinflammatory genes targeted by oolong tea theasinensin A in macrophages.
abstract: Theasinensin A is one of the oolong tea theasinensins, which differ from green tea catechins and black tea theaflavins. In a previous study, we found that theasinesin A had a potential effect on antiinflammation since theasinensin A suppressed LPS-induced COX2 and PGE(2) production. To clarify the molecular mechanisms, we investigated the gene expression profiling in macrophage-like cells treated with theasinensin A through a genome-wide DNA microarray in the present study. Among 22,050 oligonucleotides, the expression levels of 406 genes were increased by ≥3-fold in LPS-activated RAW264 cells, 259 gene signals of which were attenuated by theasinensin A treatment (≥2-fold). Expression levels of 717 genes were decreased by ≥3-fold in LPS-activated cells, of which 471 gene signals were restored by theasinensin A treatment (≥2-fold). These genes were further categorized as "defense, inflammatory response, cytokines activities, and receptor activities," and some of them were confirmed by real-time polymerase chain reaction. Furthermore, pathways analysis revealed that theasinensin A regulated the relevant expression networks of chemokines, interleukins, and interferons to exert its antiinflammatory effects.
authors.affiliation: Course of Biological Science and Technology, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Japan.
authors.forename: Jihua
authors.initials: J
authors.lastname: Chen
authors.forename: Si
authors.initials: S
authors.lastname: Qin
authors.forename: Jiping
authors.initials: J
authors.lastname: Xiao
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Tanigawa
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Uto
authors.forename: Fumio
authors.initials: F
authors.lastname: Hashimoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujii
authors.forename: De-Xing
authors.initials: DX
authors.lastname: Hou
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001578:Benzopyrans / Q000494:pharmacology*; D002460:Cell Line; D018925:Chemokines / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D007372:Interferons / Q000235:genetics / Q000378:metabolism; D007378:Interleukins / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010636:Phenols / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013662:Tea / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D018925:Chemokines; D007378:Interleukins; D008070:Lipopolysaccharides; D010636:Phenols; D013662:Tea; C481298:theasinensin A; D007372:Interferons