title: Integrated sieving microstructures on microchannels for biological cell trapping and droplet formation.
abstract: We have developed a single step microfabrication method to prepare constriction microstructures on a PCB master by controlling the etching time of two microchannels separated by a finite distance that is easily attainable using imagesetters widely available in the printing industry. PDMS replica of the constriction structures present sieving microstructures (microsieves) that could be used for size-dependent trapping of microspheres, biological cells and the formation of water-in-oil droplets.
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR.
authors.forename: Wanqing
authors.initials: W
authors.lastname: Yue
authors.forename: Cheuk-Wing
authors.initials: CW
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Mengsu
authors.initials: M
authors.lastname: Yang
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008899:Mineral Oil / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D014867:Water; C013830:baysilon; D008899:Mineral Oil