title: Gold nanoparticles-based catalysis for detection of S-nitrosothiols in blood serum.
abstract: Accumulating evidence suggests that S-nitrosothiols (RSNOs) play key roles in human health and disease. To clarify their physiological functions and roles in diseases, it is necessary to promote some new techniques for quantifying RSNOs in blood and other biological fluids. Here, a new method using gold nanoparticle catalysts has been introduced for quantitative evaluation of RSNOs in blood serum. The assay involves degrading RSNOs using gold nanoparticles and detecting nitric oxide (NO) released with NO-selective electrodes. The approach displays very high sensitivity for RSNOs with a low detection limit in the picomolar concentration range (5.08 × 10(-11) mol L(-1), S/N=3) and is free from interference of some endogenous substances such as NO(2)(-) and NO(3)(-) co-existing in blood serum. A linear function of concentration in the range of (5.0-1000.0) × 10(-9) mol L(-1) has been observed with a correlation coefficient of 0.9976. The level of RSNOs in blood serum was successfully determined using the described method above. In addition, a dose-dependent effect of gold nanoparticles on the sensitivity for RSNOs detection is revealed, and thereby the approach is potentially useful to evaluate RSNOs levels in various biological fluids via varying gold nanoparticles concentration.
authors.affiliation: State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Jia
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Qiu
authors.initials: Q
authors.lastname: Tian
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Miao
authors.forename: Libo
authors.initials: L
authors.lastname: Du
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods*; D002384:Catalysis; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D026403:S-Nitrosothiols / Q000097:blood* / Q000737:chemistry*; D013997:Time Factors
chemical_list: D026403:S-Nitrosothiols; D006046:Gold