title: Production of hydrocarbon compounds by endophytic fungi Gliocladium species grown on cellulose.
abstract: Endophytic fungi belonging to the genus Gliocladium are able to degrade plant cellulose and synthesize complex hydrocarbons under microaerophilic conditions. These fungi could thus be used to produce biofuels from cellulosics without the need for hydrolytic pretreatments. Gas chromatography-mass spectrometry-solid-phase micro-extraction (GC-MS-SPME) of head space gases from Gliocladium cultures demonstrated the production of C(6)-C(19) hydrocarbons including hexane, benzene, heptane, 3,4-dimethyl hexane, 1-octene, m-xylene, 3-methyl nonane, dodecane, tridecane, hexadecane and nonadecane directly from the cellulosic biomass. Hydrocarbon production was 100-fold higher in co-cultures of Gliocladium and Escherichia coli than in pure Gliocladium cultures. The dry mycelia weight is stable at stationary period in co-culture condition which may lead to synthesize more hydrocarbons. These fungi could potentially be developed into cost-effective biocatalysts for production of biofuels.
authors.affiliation: Center for Bioproducts and Bioenergy, Bioproducts Sciences and Engineering Laboratory, Washington State University, Richland, WA 99354-1671, USA.
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahamed
authors.forename: Birgitte K
authors.initials: BK
authors.lastname: Ahring
mesh_terms: D018533:Biomass; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry; D020462:Gliocladium / Q000378:metabolism*; D006838:Hydrocarbons / Q000378:metabolism*; D052617:Solid Phase Microextraction
chemical_list: D003470:Culture Media; D006838:Hydrocarbons; D002482:Cellulose