title: Production of 1,2-propanediol from glycerol in Saccharomyces cerevisiae.
abstract: Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2- propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Korea.
authors.forename: Joon-Young
authors.initials: JY
authors.lastname: Jung
authors.forename: Hyun Shik
authors.initials: HS
authors.lastname: Yun
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Oh
mesh_terms: D019757:Carbon-Oxygen Lyases / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000235:genetics / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019946:Propylene Glycol / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D019946:Propylene Glycol; D005993:Glycerolphosphate Dehydrogenase; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D005991:Glycerol Kinase; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase; D005990:Glycerol