title: Imaging-based approaches to understanding g protein-coupled receptor signalling complexes.
abstract: In the last 10 years, imaging assays based on resonance energy transfer (RET) and protein fragment complementation have made it possible to study interactions between components of G protein-coupled receptor (GPCR) signalling complexes in living cells under physiological conditions. Here, we consider the history of such approaches, the current tools available and how they have changed our understanding of GPCR signalling. We also discuss some theoretical and methodological issues important when combining the different types of assay.
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, Canada.
authors.forename: Darlaine
authors.initials: D
authors.lastname: Pétrin
authors.forename: Terence E
authors.initials: TE
authors.lastname: Hébert
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000266:history / Q000379:methods*; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D060066:Protein Interaction Maps; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D043562:Receptors, G-Protein-Coupled