title: [Effect of lipopolysaccharide and hypoxia on rat neutrophils in vitro].
abstract: The main aim of this study was to examine the role of lipopolysaccharide (LPS) and hypoxia on respiratory burst in rat neutrophils in vitro. Hypoxia (2% oxygen tension) inhibited respiratory burst of neutrophils in response to phorbol ester. Neutrophils treatment by LPS and hypoxia resulted in significant augmentation respiratory burst via NADPH oxidase activity in luminol chemiluminescence. This paper suggests that induction of iNOS was responsible for this effect.
authors.affiliation: Katedra Farmakologii, Uniwersytet Jagielloński Collegium Medicum, Kraków. m.salwinska@interia.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Salwińska
authors.forename: Danuta
authors.initials: D
authors.lastname: Uracz
authors.forename: Wojciech
authors.initials: W
authors.lastname: Uracz
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008163:Luminescent Measurements; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D016897:Respiratory Burst / Q000187:drug effects / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides; D019255:NADPH Oxidases