title: Interpenetrating polymer network (IPN) scaffolds of sodium hyaluronate and sodium alginate for chondrocyte culture.
abstract: In this study, porous sodium hyaluronic acid/sodium alginate (HA/SA) scaffold based on interpenetrating polymeric network (IPN) technique has been fabricated, where HA and SA were cross-linked with poly(ethylene glycol) diglycidyl ether (PEGDG) and calcium chloride, respectively. The mean pore size and the swelling ratio of fabricated scaffolds decreased, and the compressive strength increased as the content of SA increased in HA/SA IPN scaffold. Rabbit chondrocytes were seeded within the HA/SA IPN scaffolds, and then their proliferation as well as chondrogenic differentiation was examined. DNA contents observed from the chondrocytes cultured in the IPN scaffolds increased with time over 21 days, which demonstrated that the rabbit chondrocytes continued to proliferate in HA/SA scaffolds. Results of the 1,9-dimethylmethylene blue (DMMB) and p-dimethylaminobenzaldehyde (DMBA) assays showed that glycosaminoglycan (s-GAG) and collagen contents increased over culture period, indicating the chondrogenic differentiation in the scaffold. Reverse transcription-polymerase chain reaction (RT-PCR) results showed the expression of type II collagen, the main chondrogenic differentiation marker. The bands indicating mRNA expression of type II collagen increased with the culture period. These results demonstrated that the porous HA/SA IPN scaffolds were successfully prepared and could serve as an effective delivery system of the three-dimensional culture of chondrocytes.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Chung-Wook
authors.initials: CW
authors.lastname: Chung
authors.forename: Jeong Yeon
authors.initials: JY
authors.lastname: Kang
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Yoon
authors.forename: Hyung-Don
authors.initials: HD
authors.lastname: Hwang
authors.forename: Prabagar
authors.initials: P
authors.lastname: Balakrishnan
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Kyu-Don
authors.initials: KD
authors.lastname: Chung
authors.forename: Dae-Hwan
authors.initials: DH
authors.lastname: Kang
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002122:Calcium Chloride / Q000737:chemistry; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000737:chemistry; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006820:Hyaluronic Acid; D003094:Collagen; D002122:Calcium Chloride; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue