title: An abiotic fluorescent probe for cardiac troponin I.
abstract: The first ratiometric fluorescent reporter was designed for the detection of cardiac troponin I (cTnI), a key protein elicited during cardiac muscle cell death. In designing this abiotic fluorescent probe, docking simulation studies were performed to predict the probe/protein interactions along the solvent exposed regions of cTnI. Simple cuvette titration experiments in aqueous buffered solution indicate remarkable selectivity for cardiac troponin in the clinically relevant nM region versus skeletal troponin.
authors.affiliation: Department of Chemistry, New Mexico Institute of Mining & Technology, Socorro, New Mexico 87801, United States.
authors.forename: Premchendar
authors.initials: P
authors.lastname: Nandhikonda
authors.forename: Michael D
authors.initials: MD
authors.lastname: Heagy
mesh_terms: D015415:Biomarkers / Q000097:blood / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D032389:Myocytes, Smooth Muscle / Q000737:chemistry* / Q000378:metabolism; D019210:Troponin I / Q000097:blood / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D005456:Fluorescent Dyes; D019210:Troponin I