title: S-methyl thioesters are produced from fatty acids and branched-chain amino acids by brevibacteria: focus on L-leucine catabolic pathway and identification of acyl-CoA intermediates.
abstract: Despite their importance as potent odors that contribute to the aroma of numerous cheeses, S-methyl thioesters formation pathways have not been fully established yet. In a first part of our work, we demonstrated that Brevibacterium antiquum and Brevibacterium aurantiacum could produce S-methyl thioesters using short-chain fatty acids or branched-chain amino acids as precursors. Then, we focused our work on L-leucine catabolism using liquid chromatography tandem mass spectrometry and gas chromatography-mass spectrometry analyses coupled with tracing experiments. For the first time, several acyl-CoAs intermediates of the L-leucine to thioesters conversion pathway were identified. S-methyl thioisovalerate was produced from L-leucine, indicating that this amino acid was initially transaminated. Quite interestingly, data also showed that other S-methyl thioesters, e.g., S-methyl thioacetate or S-methyl thioisobutyrate, were produced from L-leucine. Enzymatic and tracing experiments allowed for postulating catabolic pathways leading to S-methyl thioesters biosynthesis.
authors.affiliation: Bio Springer, 103 Rue Jean Jaurès, Maisons-Alfort, Paris, France.
authors.forename: Alain M
authors.initials: AM
authors.lastname: Sourabié
authors.forename: Henry-Eric
authors.initials: HE
authors.lastname: Spinnler
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Bourdat-Deschamps
authors.forename: Richard
authors.initials: R
authors.lastname: Tallon
authors.forename: Sophie
authors.initials: S
authors.lastname: Landaud
authors.forename: Pascal
authors.initials: P
authors.lastname: Bonnarme
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000597:Amino Acids, Branched-Chain / Q000378:metabolism*; D001951:Brevibacterium / Q000378:metabolism*; D002611:Cheese / Q000382:microbiology; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005227:Fatty Acids / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism*; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D013457:Sulfur Compounds / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D000597:Amino Acids, Branched-Chain; D005227:Fatty Acids; D013457:Sulfur Compounds; D007930:Leucine