title: A Unique BSL-3 Cryo-Electron Microscopy Laboratory at UTMB.
abstract: This article describes a unique cryo-electron microscopy (CryoEM) facility to study the three-dimensional organization of viruses at biological safety level 3 (BSL-3). This facility, the W. M. Keck Center for Virus Imaging, has successfully operated for more than a year without incident and was cleared for select agent studies by the Centers for Disease Control and Prevention (CDC). Standard operating procedures for the laboratory were developed and implemented to ensure its safe and efficient operation. This facility at the University of Texas Medical Branch (Galveston, TX) is the only such BSL-3 CryoEM facility approved for select agent research.
authors.affiliation: University of Texas Medical Branch at Galveston, Galveston, Texas.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Sherman
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Freiberg
authors.forename: Dennis
authors.initials: D
authors.lastname: Razmus
authors.forename: Shintaro
authors.initials: S
authors.lastname: Yazuka
authors.forename: Craig
authors.initials: C
authors.lastname: Koht
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Hilser
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Lemon
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Brocard
authors.forename: Dee
authors.initials: D
authors.lastname: Zimmerman
authors.forename: Wah
authors.initials: W
authors.lastname: Chiu
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Watowich
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver