title: Role of aquaporin 3 in development, subtypes and activation of dendritic cells.
abstract: Dendritic cells (DCs) uptake soluble antigens and large volumes of fluid through macropinocytosis and migrate for antigen presentation. Aquaporin 3 (AQP3), a water and glycerol transporting protein, is highly expressed in immature DCs. To elucidate the role of AQP3 in DC function, we investigated subtype and activation of DCs in AQP3 knock-out (AQP3(-/-)) mice. Depletion of AQP3 did not affect the development of bone marrow-derived DCs (BM-DCs) by GM-CSF or the Flt3 ligand and the level of expression of CD86 on unstimulated and LPS-stimulated BM-DCs. In addition, the percentage of CD86(+) cells among splenic cDCs after LPS treatment in both in vitro and in vivo conditions was similar in wild type and AQP3(-/-) mice. However, the frequency of CD4(+) cDCs in the spleen of AQP3(-/-) mice was significantly lower than that of wild type mice. There was higher expression of CD103 in the CD8(+) subpopulation of splenic cDCs from AQP3(-/-) mice than wild type mice. In the dermis, more CD103-expressing cells were detected in AQP3(-/-) mice than in wild type mice and the LPS-induced decrease of CD103(+) dermal DCs was impaired in AQP3(-/-) mice. AQP3 depletion did not affect the uptake of either albumin or dextran by CD11c(+) splenic DCs. However, HgCl(2), which is an AQP inhibitor, significantly inhibited the uptake of albumin but not dextran by CD11c(+) splenic DCs. These results suggest that AQP3 may play a role in modulating DC population and migration.
authors.affiliation: Department of Biochemistry, School of Medicine, Dong-A University, Busan 602-714, Republic of Korea.
authors.forename: Min-Gyu
authors.initials: MG
authors.lastname: Song
authors.forename: Seung-Young
authors.initials: SY
authors.lastname: Hwang
authors.forename: Joo-In
authors.initials: JI
authors.lastname: Park
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
authors.forename: Hae-Rahn
authors.initials: HR
authors.lastname: Bae
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D051400:Aquaporin 3 / Q000276:immunology / Q000378:metabolism*; D002465:Cell Movement / Q000276:immunology; D002469:Cell Separation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D004705:Endocytosis / Q000276:immunology; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D018613:Microscopy, Confocal
chemical_list: C496028:Aqp3 protein, mouse; D051400:Aquaporin 3