title: Magnetic and fluorescent glycopolymer hybrid nanoparticles for intranuclear optical imaging.
abstract: The synthesis of galactose-displaying core-shell nanospheres exhibiting both fluorescent and magnetic properties by grafting a glycocopolymer consisting of 6-O-methacryloylgalactopyranose (MAGal) and 4-(pyrenyl)butyl methacrylate (PyMA) onto magnetic silica particles via thiol-ene chemistry is reported. Magnetization measurements indicated that neither the encapsulation of the iron oxide particles into silica nor the grafting of the glycocopolymer chains had a significant influence on the superparamagnetic properties. This not only simplifies the purification of the particles but may facilitate the use of the particles in applications such as hyperthermia or magnetic resonance imaging (MRI). Furthermore, the hydrophilic glycopolymer shell provided solubility of the particles in aqueous medium and enabled the uptake of the particles into the cytoplasm and nucleus of lung cancer cells via carbohydrate-lectin recognition effects.
authors.affiliation: Makromolekulare Chemie II, Universität Bayreuth, 95440 Bayreuth, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Pfaff
authors.forename: Anja
authors.initials: A
authors.lastname: Schallon
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Ruhland
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Majewski
authors.forename: Holger
authors.initials: H
authors.lastname: Schmalz
authors.forename: Ruth
authors.initials: R
authors.lastname: Freitag
authors.forename: Axel H E
authors.initials: AH
authors.lastname: Müller
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000378:metabolism; D004705:Endocytosis / Q000502:physiology; D005290:Ferric Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis; D005690:Galactose / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure; D054118:Nanospheres / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011721:Pyrenes / Q000032:analysis / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D008689:Methacrylates; D011721:Pyrenes; D013438:Sulfhydryl Compounds; C000499:ferric oxide; D012822:Silicon Dioxide; C016284:butyl methacrylate; D005690:Galactose