title: Development of peritoneal macrophage along a dendritic cell lineage in response to uptake of oligomannose-coated liposomes.
abstract: In this study, we investigate the potential of peritoneal macrophages to differentiate into dendritic cell (DCs) in response to preferential uptake of oligomannose-coated liposomes (OMLs). About 30% of peritoneal cells (PECs) preferentially took up OMLs that were administered into the peritoneal cavity. The OML-ingesting cells expressed CD11b and F4/80, but lacked CD11c expression, indicating that the OML-ingesting PECs with a CD11b(high)CD11c(-) phenotype are resident peritoneal macrophages. During in vitro cultivation, CD11c(+) cells arose among the PECs with ingested OMLs. CD11c(+) cells also developed among enriched peritoneal CD11b(high)CD11(-) cells from OML-treated mice, and the resulting CD11c(+) cells expressed co-stimulatory molecules and MHC class II. In addition, OML-ingesting CD11b(high)CD11c(+) cells were found in spleen after the enriched peritoneal macrophages with ingested OMLs were transplanted in the peritoneal cavity of mice. These results show that a fraction of peritoneal macrophages can differentiate into mature DCs following uptake of OMLs.
authors.affiliation: Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa, Japan. naoyaki@keyaki.cc.u-tokai.ac.jp
authors.forename: Naoya
authors.initials: N
authors.lastname: Kojima
authors.forename: Chiaki
authors.initials: C
authors.lastname: Kato
authors.forename: Megumi
authors.initials: M
authors.lastname: Igarashi
authors.forename: Mariko
authors.initials: M
authors.lastname: Ishii
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D039521:CD11c Antigen / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology*; D005260:Female; D008081:Liposomes; D017737:Macrophages, Peritoneal / Q000166:cytology* / Q000276:immunology* / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D039481:CD11b Antigen; D039521:CD11c Antigen; D008081:Liposomes; D009844:Oligosaccharides; C028569:oligomannoside