title: A mesophilic Clostridium species that produces butanol from monosaccharides and hydrogen from polysaccharides.
abstract: A unique mesophilic Clostridium species strain BOH3 is obtained in this study, which is capable of fermenting monosaccharides to produce butanol and hydrolyzing polysaccharides to produce hydrogen (H(2)) and volatile fatty acids (VFAs). From 30 g/L of glucose and xylose each, batch culture BOH3 was able to produce 4.67 and 4.63 g/L of butanol. Enhancement treatments by increasing the inoculated cells improved butanol production to 7.05 and 7.41 g/L, respectively. Hydrogen production (2.47 and 1.93 mmol) was observed when cellulose and xylan (10 g/L each) were used, suggesting that strain BOH3 possesses xylanolytic and cellulolytic capabilities. These unique features reveal the strain's novelty as most wild-type solventogenic strains have not been reported to have such properties. Therefore, culture BOH3 is promising in generating butanol and hydrogen from renewable feedstock.
authors.affiliation: Department of Civil and Environmental Engineering, National University of Singapore, Block E2-02-13, 1 Engineering Drive 3, Singapore 117576, Singapore.
authors.forename: Sandhi Eko
authors.initials: SE
authors.lastname: Bramono
authors.forename: Yuen Sean
authors.initials: YS
authors.lastname: Lam
authors.forename: Say Leong
authors.initials: SL
authors.lastname: Ong
authors.forename: Jianzhong
authors.initials: J
authors.lastname: He
mesh_terms: D000440:Butanols / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D003013:Clostridium / Q000145:classification / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006868:Hydrolysis; D009005:Monosaccharides / Q000378:metabolism*; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism
chemical_list: D000440:Butanols; D009005:Monosaccharides; D011134:Polysaccharides; D014990:Xylans; D006859:Hydrogen; D002482:Cellulose