title: Monoclonal antibody against NRP-1 b1b2.
abstract: Neuropilin-1 is a member of the neuropilins family protein, which contains a large extracellular domain (a1a2, b1b2 and c), a single transmembrane domain, and a short cytoplasmic tail. NRP-1 plays a critical role in angiogenesis and stimulates endothelial cell division and migration by binding VEGF(165) with b1b2 domain. In the present study, we report the establishment of a monoclonal antibody (A6-26-11-26 clone) specific for NRP-1 b1b2 through hybridoma method. Western blot analysis indicates that our NRP-1 b1b2 MAb can combine both NRP-1 b1b2 and NRP-1 originated from tumor cells. This monoclonal antibody against NRP-1 b1b2 will be useful in the further development of cancer target strategy.
authors.affiliation: Cancer Research Center, Medical College, Xiamen University, 422 Siming South Road, Xiamen, Fujian, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Fanghong
authors.initials: F
authors.lastname: Luo
authors.forename: Shengyu
authors.initials: S
authors.lastname: Wang
authors.forename: Erru
authors.initials: E
authors.lastname: Ni
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Tang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Lv
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Chen
authors.forename: Lukui
authors.initials: L
authors.lastname: Chen
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000918:Antibody Specificity; D015153:Blotting, Western; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D039942:Neuropilin-1 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D011994:Recombinant Proteins; D039942:Neuropilin-1