title: The adhesion and proliferation of bone marrow-derived mesenchymal stem cells promoted by nanoparticle surface.
abstract: This study's aim consists of evaluating the adhesion and proliferation of mesenchymal stem cells (MSCs) derived from rat bone marrow on nanoparticle Titanium (Ti) surface. Hence, passage 3 MSCs were, respectively, seeded on nanoparticle Ti and pure Ti surfaces and then cultured for 32 h. Cell morphology and viability were separately examined by scanning electron microscopy and 3-(4,5-dimethylthiazsol-2-yl)-2,5-diphenyltetrazolium bromide assay. Moreover, the mitotic rate of the attached MSCs was observed through immunocytochemistry. The real-time polymerase chain reaction was applied to determine the adhesion-associated messenger ribonucleic acid (mRNA), CD44 gene encoding variant isoform 6 (CD44 V6), and the integrinβ1 level. The results showed that MSCs performed better in faster extension on the nanoparticle Ti surface than on the pure Ti surface after culturing for 4 h, and were quicker in fusion patterns after 16 h. Furthermore, cell viability was significantly increased on the nanoparticle Ti surface compared to that of the pure Ti surface 16 h after initial seeding (p < 0.05), and the mitotic rate of attached MSCs on the nanoparticle Ti surface was higher than that on the pure Ti surface after 32 h (p < 0.05). More interestingly, the CD44 V6 and integrinβ1 mRNA in the nanoparticle Ti surface group expressed higher than that in the pure Ti surface group after 4 h (p < 0.05), and positive correlation between CD44 V6 and integrinβ1 was found through statistical analysis (correlation coefficient r (s) = 0.98, p < 0.05). Our study's result indicates that a nanoparticle Ti surface can significantly promote the adhesion and proliferation of MSCs, and also improve the bioactivity of Ti surface.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, Jiangsu 215006, People's Republic of China.
authors.forename: Lian
authors.initials: L
authors.lastname: Chen
authors.forename: Junying
authors.initials: J
authors.lastname: Sun
authors.forename: Zhansheng
authors.initials: Z
authors.lastname: Zhu
authors.forename: Kaiyun
authors.initials: K
authors.lastname: Wu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Li
authors.forename: Hongming
authors.initials: H
authors.lastname: Liu
authors.forename: Shi
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D017931:DNA Primers; D005434:Flow Cytometry; D018960:Hyaluronan Receptors / Q000378:metabolism; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D015854:Up-Regulation
chemical_list: D017931:DNA Primers; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D014025:Titanium