title: [Mechanism of E1A-mediated escape from ras-induced senescence in human fibraoblasts].
abstract: OBJECTIVE
  To study the effect of binding activities of the NH(2) terminus of E1A to the proteins regulating cell growth on ras-induced cell senescence and explore the mechanism of E1A-mediated escape from ras-induced senescence by E1A in human fibroblast.
  
  
  METHODS
  In primary human fibroblasts, the proteins regulating cell growth in association with E1A NH(2) terminus, including the Rb family proteins, p300/CBP, and p400, were inactivated or interfered. The effect of alterations in the binding activities of these proteins on cell senescence bypass mediated by E1A was evaluated by cell growth curve.
  
  
  RESULTS
  The Inactivation of Rb family proteins alone was not sufficient to rescue ras-induced cell senescence, whereas inactivation of both the Rb proteins and p300/CBP blocked ras-induced senescence of human fibroblasts.
  
  
  CONCLUSIONS
  Rb and p300/CBP binding activities are both required for E1A to bypass ras-induced senescence in human fibroblasts.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lei@fimmu.com
authors.forename: Yi-lei
authors.initials: YL
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Yu
mesh_terms: D017500:Adenovirus E1A Proteins / Q000494:pharmacology*; D016922:Cellular Senescence / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D061251:Primary Cell Culture; D016160:Retinoblastoma Protein / Q000378:metabolism; D012867:Skin / Q000166:cytology; D050880:p300-CBP Transcription Factors / Q000378:metabolism; D018631:ras Proteins / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D017500:Adenovirus E1A Proteins; D016160:Retinoblastoma Protein; D050880:p300-CBP Transcription Factors; D018631:ras Proteins