title: Molecular characterization of Staphylococcus pseudintermedius strains isolated from clinical samples of animal origin.
abstract: The aim of this study was to determine the species distribution among 44 randomly selected clinical isolates (30 mecA-positive and 14 mecA-negative) of animal origin previously identified as Staphylococcus intermedius by phenotypic tests and species-specific PCR amplification of the 16S rRNA gene. For this purpose, we used a multiplex PCR for the detection of the nuc gene and restriction fragment length polymorphism analysis of pta gene amplified by PCR. Both methods allow discrimination of Staphylococcus pseudintermedius from the other closely related members of the S. intermedius group and other coagulase-positive staphylococci isolated from animals. Genetic diversity of S. pseudintermedius strains was analyzed by staphylococcal protein A-encoding gene (spa) typing. Multiplex PCR method was used to identify staphylococcal cassette chromosome mec (SCCmec) type in mecA-positive strains. All isolates previously identified as S. intermedius were shown to belong to S. pseudintermedius. According to PCR-based SCCmec typing, SCCmecIII was the most prevalent type (n = 23), and solely seven isolates were designated as non-typeable. Furthermore, the assessment of spa-typing results revealed that the majority of all strains (n = 27) harbored spa type t02, and 17 strains were classified as non-typeable.
authors.affiliation: Division of Bacteriology and Molecular Biology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland. dorota.chrobak@wp.pl
authors.forename: D
authors.initials: D
authors.lastname: Chrobak
authors.forename: M
authors.initials: M
authors.lastname: Kizerwetter-Świda
authors.forename: M
authors.initials: M
authors.lastname: Rzewuska
authors.forename: A
authors.initials: A
authors.lastname: Moodley
authors.forename: L
authors.initials: L
authors.lastname: Guardabassi
authors.forename: M
authors.initials: M
authors.lastname: Binek
mesh_terms: D000820:Animal Diseases / Q000453:epidemiology / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015373:Bacterial Typing Techniques; D001826:Body Fluids / Q000382:microbiology; D002415:Cats; D002417:Cattle; D004285:Dogs; D024881:Drug Resistance, Bacterial; D005849:Gerbillinae; D008836:Micrococcal Nuclease / Q000032:analysis / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction; D046915:Penicillin-Binding Proteins; D011044:Poland / Q000453:epidemiology; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000662:veterinary*; D013205:Staphylococcal Protein A / Q000032:analysis / Q000235:genetics; D057789:Staphylococcus intermedius / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D012336:RNA, Ribosomal, 16S; D013205:Staphylococcal Protein A; C487789:mecA protein, Staphylococcus aureus; C074002:nuc protein, staphylococcus; D008836:Micrococcal Nuclease