title: Phosphonoacetate biosynthesis: in vitro detection of a novel NADP(+)-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of the marine Roseovarius nubinhibens ISM.
abstract: A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate hydrolase activities. These findings indicate the existence of a biological route from 2-AEP via phosphonoacetaldehyde for the production of phosphonoacetate, which has not previously been shown to be a natural product. The three enzymes appear to constitute a previously-unreported pathway for the mineralization of 2-AEP which is a potentially important source of phosphorus in the nutrient-stressed marine environment.
authors.affiliation: School of Biological Science, Queens University, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, Northern Ireland, UK.
authors.forename: N A
authors.initials: NA
authors.lastname: Cooley
authors.forename: A N
authors.initials: AN
authors.lastname: Kulakova
authors.forename: J F
authors.initials: JF
authors.lastname: Villarreal-Chiu
authors.forename: J A
authors.initials: JA
authors.lastname: Gilbert
authors.forename: J W
authors.initials: JW
authors.lastname: McGrath
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000615:Aminoethylphosphonic Acid / Q000378:metabolism*; D059001:Aquatic Organisms / Q000201:enzymology / Q000254:growth & development; D001426:Bacterial Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009249:NADP / Q000378:metabolism; D010746:Phosphonoacetic Acid / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism; D041902:Rhodobacteraceae / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D013379:Substrate Specificity; D013696:Temperature; D000637:Transaminases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C003126:phosphonoacetaldehyde; D010758:Phosphorus; D009249:NADP; D000615:Aminoethylphosphonic Acid; D000637:Transaminases; D000469:Alkaline Phosphatase; C097254:phosphonoacetate hydrolase; D000079:Acetaldehyde; D010746:Phosphonoacetic Acid