title: Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds.
abstract: Osteochondral defects are prone to induce osteoarthritic degenerative changes. Many tissue-engineering approaches that aim to generate osteochondral implants suffer from poor tissue formation and compromised integration. This illustrates the need for further improvement of heterogeneous tissue constructs. Engineering of these structures is expected to profit from strategies addressing the complexity of tissue organization and the simultaneous use of multiple cell types. Moreover, this enables the investigation of the effects of three-dimensional (3D) organization and architecture on tissue function. In the present study, we characterize the use of a 3D fiber deposition (3DF) technique for the fabrication of cell-laden, heterogeneous hydrogel constructs for potential use as osteochondral grafts. Changing fiber spacing or angle of fiber deposition yielded scaffolds of varying porosity and elastic modulus. We encapsulated and printed fluorescently labeled human chondrocytes and osteogenic progenitors in alginate hydrogel yielding scaffolds of 1×2 cm with different parts for both cell types. Cell viability remained high throughout the printing process, and cells remained in their compartment of the printed scaffold for the whole culture period. Moreover, distinctive tissue formation was observed, both in vitro after 3 weeks and in vivo (6 weeks subcutaneously in immunodeficient mice), at different locations within one construct. These results demonstrate the possibility of manufacturing viable centimeter-scaled structured tissues by the 3DF technique, which could potentially be used for the repair of osteochondral defects.
authors.affiliation: Department of Orthopaedics, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Natalja E
authors.initials: NE
authors.lastname: Fedorovich
authors.forename: Wouter
authors.initials: W
authors.lastname: Schuurman
authors.forename: Hans M
authors.initials: HM
authors.lastname: Wijnberg
authors.forename: Henk-Jan
authors.initials: HJ
authors.lastname: Prins
authors.forename: P René
authors.initials: PR
authors.lastname: van Weeren
authors.forename: Jos
authors.initials: J
authors.lastname: Malda
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Alblas
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology*; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D016062:Porosity; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014783:Viscosity
chemical_list: D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate