title: Intestinal parasitic infections in an industrialized country; a new focus on children with better DNA-based diagnostics.
abstract: In recent years, the isolation of parasitic DNA from faecal samples and PCR techniques, have been improved and simplified. Moreover, the introduction of real-time PCR has made it possible to multiplex different targets into one reaction. These new technical possibilities make it feasible to introduce PCR with its unsurpassed sensitivity and specificity in a routine laboratory setting for the diagnosis of intestinal parasites. Detection rates of the parasitic infections included in the PCR are increased significantly compared with microscopy. Molecular diagnostics, especially in children, reveal a possible cause of the gastrointestinal complaints in many more cases compared with conventional methods. Usually in GP patients no other pathogenic parasites are detected using microscopy and in the returning travellers additional parasites are found with microscopy in a minority of cases only. Multiplex real-time PCR offers a highly sensitive and specific diagnostic alternative for labour intensive microscopy in clinical laboratory practice. Additional diagnostic methods for the detection of parasitic infections that are not included as PCR target can be limited to a selected group of patients.
authors.affiliation: Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands. j.j.verweij@lumc.nl
authors.forename: Jaco J
authors.initials: JJ
authors.lastname: Verweij
authors.forename: Lisette
authors.initials: L
authors.lastname: van Lieshout
mesh_terms: D000293:Adolescent; D000328:Adult; D001530:Belgium / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000469:parasitology; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D019049:Developed Countries; D004029:Dientamoeba / Q000235:genetics / Q000302:isolation & purification*; D004030:Dientamoebiasis / Q000175:diagnosis / Q000469:parasitology; D004404:Dysentery, Amebic / Q000175:diagnosis / Q000469:parasitology; D004748:Entamoeba histolytica / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000469:parasitology; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009426:Netherlands / Q000453:epidemiology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan