title: Detection and characterization of 2-E2-specific surface protein in human pluripotent stem cells.
abstract: To investigate cell surface antigens on human embryonic stem cells (hESCs), we generated a panel of monoclonal antibodies (MAbs) against undifferentiated hESCs by a decoy immunization strategy. One of the MAbs, MAb 2-E2, specifically bound to human pluripotent stem cells but not to mouse pluripotent stem cells and mouse embryonic fibroblasts. 2-E2 also bound to human differentiated cells, peripheral blood monocytes, and dermal fibroblasts. 2-E2 antigen expression drastically decreased in retinoic acid-induced differentiated hESCs. However, it gradually increased after initial decrease during embryoid body formation of hESCs. 2-E2 recognized approximately 68 and 27 kDa proteins present on the surface of human pluripotent stem cells. The results suggest that 2-E2-specific surface protein is a novel cell surface protein that plays a role in the early differentiation of human pluripotent stem cells.
authors.affiliation: Insitute of Bioscience, Department of Bioscience and Biotechnology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul, Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Kim
authors.forename: Hong Seo
authors.initials: HS
authors.lastname: Choi
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Chun Jeih
authors.initials: CJ
authors.lastname: Ryu
mesh_terms: D000071443:12E7 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000071443:12E7 Antigen; D015703:Antigens, CD; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules