title: Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B).
abstract: Impaired trafficking of human kidney anion exchanger 1 (kAE1) to the basolateral membrane of α-intercalated cells of the kidney collecting duct leads to the defect of the Cl(-)/HCO(3)(-) exchange and the failure of proton (H(+)) secretion at the apical membrane of these cells, causing distal renal tubular acidosis (dRTA). In the sorting process, kAE1 interacts with AP-1 mu1A, a subunit of AP-1A adaptor complex. However, it is not known whether kAE1 interacts with motor proteins in its trafficking process to the plasma membrane or not. We report here that kAE1 interacts with kinesin family member 3B (KIF3B) in kidney cells and a dileucine motif at the carboxyl terminus of kAE1 contributes to this interaction. We have also demonstrated that kAE1 co-localizes with KIF3B in human kidney tissues and the suppression of endogenous KIF3B in HEK293T cells by small interfering RNA (siRNA) decreases membrane localization of kAE1 but increases its intracellular accumulation. All results suggest that KIF3B is involved in the trafficking of kAE1 to the plasma membrane of human kidney α-intercalated cells.
authors.affiliation: Medical Molecular Biology Unit, Office for Research and Development Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
authors.forename: Natapol
authors.initials: N
authors.lastname: Duangtum
authors.forename: Mutita
authors.initials: M
authors.lastname: Junking
authors.forename: Nunghathai
authors.initials: N
authors.lastname: Sawasdee
authors.forename: Boonyarit
authors.initials: B
authors.lastname: Cheunsuchon
authors.forename: Thawornchai
authors.initials: T
authors.lastname: Limjindaporn
authors.forename: Pa-thai
authors.initials: PT
authors.lastname: Yenchitsomanus
mesh_terms: D020816:Amino Acid Motifs; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D007668:Kidney / Q000378:metabolism*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; C095899:KIF3B protein, human; C454201:SLC4A1 protein, human; D016547:Kinesins