title: Expression of bone morphogenetic proteins and their receptors in the normal adult rat spinal cord.
abstract: OBJECTIVE
  To observe the expression distribution of bone morphogenetic proteins (BMP) in the spinal cord of normal adult rats.
  
  
  METHODS
  Expression of BMP2, BMP4, and BMP7, and their receptors BMPR Ia, BMPR Ib, and BMP II were detected by immunochemistry analysis in the spinal cord of normal adult rats.
  
  
  RESULTS
  Expression of BMPR Ia or BMPR Ib was observed in the motor neurons of the anterior horn, sensory neurons of the dorsal horn, oligodentrocytes, some microglia, and some astrocytes. Expression of receptor BMPR II was found in the oligodentrocytes and motor neurons in the gray matter of anterior horn. It was also expressed in some glial fibrillary acidic protein (GFAP)-positive astrocytes in the white matter but not in the gray matter. BMP2 and BMP4 were not expressed in the spinal cord of normal adult rats by immunohistochemistry. BMP7 was expressed in all the APC-positive oligodentrocytes, all the NeuN-positive motor neurons in the anterior horn, and some astrocytes in the normal spinal cord. Phosphated pSmad 1/5/8 protein was expressed in all the oligodentrocytes, all the neurons, and some astrocytes, especially in the GFAP-positive astrocytes which were RC2-positive radial glia in the subventricular zone.
  
  
  CONCLUSIONS
  BMP7, BMP receptors, and phosphated pSmad 1/5/8 are expressed in many types of cells whereas BMP2 and BMP4 are not expressed in the spinal cord of normal adult rats, which suggests an important function of BMP signal pathway in the neuron and glia of spinal cord.
authors.affiliation: Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Guixiu
authors.initials: G
authors.lastname: Yuan
authors.forename: Liwen
authors.initials: L
authors.lastname: Li
authors.forename: Dingquan
authors.initials: D
authors.lastname: Zou
authors.forename: Xinghua
authors.initials: X
authors.lastname: Jiang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.forename: Junmei
authors.initials: J
authors.lastname: Xu
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism*; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics / Q000378:metabolism*; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D052004:Bone Morphogenetic Protein Receptors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013116:Spinal Cord / Q000378:metabolism*
chemical_list: C525720:Bmp2 protein, rat; C525811:Bmp4 protein, rat; C525821:Bmp7 protein, rat; D055396:Bone Morphogenetic Protein 2; D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D052004:Bone Morphogenetic Protein Receptors