title: A novel magnetic mesoporous silica packed S-shaped microfluidic reactor for online proteolysis of low-MW proteome.
abstract: Magnetic mesoporous silica with a magnetic cover and mesoporous core was synthesized, filled with trypsin and located in an S-shaped microfluidic reactor. High-molecular weight (MW) proteins were split to waste by fractionation, whilst low-MW proteins were retained on the chip to be digested.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
authors.forename: Qianhao
authors.initials: Q
authors.lastname: Min
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhang
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D008280:Magnetics; D046210:Microfluidic Analytical Techniques; D008970:Molecular Weight; D011095:Polyethylenes / Q000737:chemistry; D016062:Porosity; D011506:Proteins / Q000378:metabolism; D059748:Proteolysis; D020543:Proteome / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D011095:Polyethylenes; D011506:Proteins; D020543:Proteome; D000644:Quaternary Ammonium Compounds; C509969:SBA-15; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012822:Silicon Dioxide; D014357:Trypsin; D052203:Ferrosoferric Oxide