title: [Altered collagene characteristics and lysyl oxidase activity in lathyrism].
abstract: Experiments were carried out on rats with lathyrism, which was induced by adding semicarbazide (0.075%) into drinking water for 45 days. The data obtained show a 30% reduction in the body weight and an increase in.organ weight coefficients. Semicarbazide intake led to the pelvic limb paralysis, scoliosis, bone tissue degradation, cartilage growth, 46% decrease of the calcium level in the femur. It has been detected essential structural changes in extracellular matrix based on the collagen cross-links reduction. The activity of lysyl oxidase, a key enzyme for the collagen development, showed 5-fold decrease in the aorta tissues. The level of formaldehyde, a nonenzymic cross-links developer, has been measured in the liver tissue by the aldehyde trap (5,5-dimethyleyclohexane-1,3-dione) administration and then fluorimetric determination of formaldimedone. Under semicarbazide load, the formaldehyde level in the liver tissue was reduced by 47%. Therefore, semicarbazide influences not only the enzymic development of aldehyde groups in collagen, but the level of other aldehydes, which can cause cross-links. This experimental model of lathyrism is appropriate for investigation of the lysyl oxidase inhibitors effect on extracellular matrix.
authors.forename: T T
authors.initials: TT
authors.lastname: Volodina
authors.forename: N D
authors.initials: ND
authors.lastname: Dzvonkevych
authors.forename: L M
authors.initials: LM
authors.lastname: Petrun'
authors.forename: I P
authors.initials: IP
authors.lastname: Krysiuk
authors.forename: N M
authors.initials: NM
authors.lastname: Popova
authors.forename: S H
authors.initials: SH
authors.lastname: Shandrenko
authors.forename: M P
authors.initials: MP
authors.lastname: Dmytrenko
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology / Q000378:metabolism; D001835:Body Weight; D001842:Bone and Bones / Q000201:enzymology / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D004195:Disease Models, Animal; D005557:Formaldehyde / Q000378:metabolism; D007596:Joints / Q000201:enzymology / Q000378:metabolism; D007842:Lathyrism / Q000139:chemically induced / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012663:Semicarbazides / Q000494:pharmacology; D012867:Skin / Q000201:enzymology / Q000378:metabolism
chemical_list: D012663:Semicarbazides; D005557:Formaldehyde; C010059:carbamylhydrazine; D003094:Collagen; D008249:Protein-Lysine 6-Oxidase