title: Genome wide expression profile in human HTR-8/Svneo trophoblastic cells in response to overexpression of placental alkaline phosphatase gene.
abstract: During pregnancy, placental growth allows the adaptation of the feto-maternal unit to fetal requirements. Placental alkaline phosphatase (PLAP) is a phosphomonoesterase produced increasingly until term by the placenta and also ectopically in some tumors. To precise the role of this enzyme in the placenta, we analyzed the genome wide expression profile of HTR-8/Svneo trophoblastic cells after overexpression of the alkaline phosphatase gene (ALPP). We showed that ALPP overexpression mainly altered expression of genes implicated in cellular growth and proliferation. These results were confirmed by the study of cellular effects in HTR-8/Svneo cells overexpressing ALPP and in HTR-8/Svneo cells in which ALPP expression was suppressed by siRNA. We showed that PLAP exerts a positive effect on DNA replication and acts as a proliferative factor in trophoblastic cells.
authors.affiliation: Unité de pathologie cellulaire et génétique UPRES-EA2493, UFR Paris Ile de France Ouest PRES Universud Paris, Université de Versailles-Saint Quentin en Yvelines, Versailles, France.
authors.forename: L
authors.initials: L
authors.lastname: Bellazi
authors.forename: E
authors.initials: E
authors.lastname: Mornet
authors.forename: G
authors.initials: G
authors.lastname: Meurice
authors.forename: N
authors.initials: N
authors.lastname: Pata-Merci
authors.forename: P
authors.initials: P
authors.lastname: De Mazancourt
authors.forename: M-N
authors.initials: MN
authors.lastname: Dieudonné
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D002460:Cell Line; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D016014:Linear Models; D020411:Oligonucleotide Array Sequence Analysis; D010920:Placenta / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*
chemical_list: D058851:GPI-Linked Proteins; D007527:Isoenzymes; D034741:RNA, Small Interfering; D012313:RNA; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental