title: Determination of some phenolic compounds in red wine by RP-HPLC: method development and validation.
abstract: A methodology employing reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for simultaneous determination of five phenolic compounds in red wine. The chromatographic separation was carried out in a C(18) column with water acidify with acetic acid (pH 2.6) (solvent A) and 20% solvent A and 80% acetonitrile (solvent B) as the mobile phase. The validation parameters included: selectivity, linearity, range, limits of detection and quantitation, precision and accuracy, using an internal standard. All calibration curves were linear (R(2) > 0.999) within the range, and good precision (RSD < 2.6%) and recovery (80-120%) was obtained for all compounds. This method was applied to quantify phenolics in red wine samples from Santa Catarina State, Brazil, and good separation peaks for phenolic compounds in these wines were observed.
authors.affiliation: Departamento de Ciência e Tecnologia de Alimentos CAL/CCA, Florianópolis, SC, Brazil.
authors.forename: Vívian Maria
authors.initials: VM
authors.lastname: Burin
authors.forename: Stefany Grützmann
authors.initials: SG
authors.lastname: Arcari
authors.forename: Léa Luzia Freitas
authors.initials: LL
authors.lastname: Costa
authors.forename: Marilde T
authors.initials: MT
authors.lastname: Bordignon-Luiz
mesh_terms: D000097:Acetonitriles; D000704:Analysis of Variance; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D002934:Cinnamates / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis*; D016018:Least-Squares Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
chemical_list: D000097:Acetonitriles; D002934:Cinnamates; D005419:Flavonoids; C032159:acetonitrile