title: Biodegradable tocopherol acetate as a drug carrier to prevent ureteral stent-associated infection.
abstract: Biomaterial-centred bacterial infections present common and challenging complications with medical implants like ureteral stent which provide substratum for the biofilm formation. Hence the purpose of this study is to make antibacterial stent surface with biodegradable polymer (tocopherol acetate) and anti-infective agents (norfloxacin and metronidazole) using a modified dip-coating procedure. This is done by impregnating the stent pieces in the anti-infective solution (a mixture of norfloxacin-metronidazole and polymer) for uniform surface coating (drug-carrier-coated stents). After coating, agar diffusion test was performed as qualitative test to find out the sensitivity of coated stents against the clinical isolates, Staphylococcus epidermidis and Escherichia coli. Quantitative test was measured by calculating the numbers of adhered bacteria on coated and uncoated stents by incubating the stent pieces in artificial urine. Difference in the number of viable bacteria adhered on the surface of coated and uncoated stents were statistically calculated using chi square test with p < 0.05 considered significant. The stent colonising ability of Staphylococcus epidermidis and Escherichia coli in a controlled environment chamber was determined using two-challenge dose of the isolates by in vitro challenge test. In qualitative test, the zone of inhibition around the coated stents showed sensitivity against the clinical isolates. In quantitative test, the number of adhered bacteria on the surface of coated stents was reduced to a significant level (p < 0.05). The polymer, tocopherol acetate is highly biodegradable in nature. Due to its degrading ability in body tissues, it releases the anti-infective drugs at a constant and sustained rate.
authors.affiliation: Department of Microbiology and Bioinformatics, CMS College of Science and Commerce, Coimbatore, India.
authors.lastname: Elayarajah
authors.forename: R
authors.initials: R
authors.lastname: Rajendran
authors.lastname: Venkatrajah
authors.forename: Sweda
authors.initials: S
authors.lastname: Sreekumar
authors.forename: Asa
authors.initials: A
authors.lastname: Sudhakar
authors.lastname: Janiga
authors.forename: Soumya
authors.initials: S
authors.lastname: Sreekumar
mesh_terms: D020341:Absorbable Implants; D000892:Anti-Infective Agents, Urinary / Q000008:administration & dosage; D001422:Bacterial Adhesion / Q000187:drug effects; D003428:Cross Infection / Q000517:prevention & control; D004337:Drug Carriers; D054855:Drug-Eluting Stents; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008795:Metronidazole / Q000008:administration & dosage; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000008:administration & dosage; D016459:Prosthesis-Related Infections / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000187:drug effects; D024505:Tocopherols; D014513:Ureter / Q000601:surgery*; D014552:Urinary Tract Infections / Q000517:prevention & control*
chemical_list: D000892:Anti-Infective Agents, Urinary; D004337:Drug Carriers; D008795:Metronidazole; D009643:Norfloxacin; D024505:Tocopherols