title: Antibacterial effectiveness of peracetic acid and conventional endodontic irrigants.
abstract: This study evaluated the in vitro antibacterial activity of conventional and experimental endodontic irrigants against Enterococcus faecalis. The following substances were evaluated by direct contact test: 2.5% sodium hypochlorite (NaOCl); 2% chlorhexidine (CHX); 1% peracetic acid. After different contact periods (30 s, 1, 3, and 10 min), a neutralizing agent was applied. Serial 10-fold dilutions were prepared and plated onto tryptic soy agar (TSA) and the number of colony-forming units per milliliter (CFU/mL) was determined. Sterile saline was used as a negative control. Both 2.5% NaOCl and 2% CHX eliminated E. faecalis after 30 s of contact. Peracetic acid reduced the bacterial counts by 86% after 3 min and completely eliminated E. faecalis after 10 min. These results allow us to conclude that 1% peracetic acid is effective against E. faecalis, despite its slower action compared with 2.5% NaOCl and 2% CHX.
authors.affiliation: Department of Restorative Dentistry, Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Juliane Maria
authors.initials: JM
authors.lastname: Guerreiro-Tanomaru
authors.forename: Renata Dornelles
authors.initials: RD
authors.lastname: Morgental
authors.forename: Norberto Batista
authors.initials: NB
authors.lastname: Faria-Junior
authors.forename: Fábio Luis Camargo Vilela
authors.initials: FL
authors.lastname: Berbert
authors.forename: Mário
authors.initials: M
authors.lastname: Tanomaru-Filho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D002710:Chlorhexidine / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D008422:Materials Testing; D050296:Microbial Viability / Q000187:drug effects; D010463:Peracetic Acid / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D010463:Peracetic Acid; D002710:Chlorhexidine