title: [Population characterization of Balantidium coli from pigs using ITS1-5.8S rRNA-ITS2 sequence].
abstract: OBJECTIVE
  To determine the species and genotypes of Balantidium isolated from pigs in Henan province, China.
  
  
  METHODS
  Scatoscopy and the modified DMEM media were used to isolate trophozoites of Balantidium from pig feces. The ITS1-5.8S rRNA-ITS2-based molecular marker method, Acridine orange staining ( AO) and microscopic observation were used to determine the population characteristics among different isolates of B. coli from various pigs farms.
  
  
  RESULTS
  We isolated 15 isolates from the pigs at diagnosis in the Animal Hospital of Henan University of Science and Technology from the pig farms of the 8 counties or cities of the west of Henan province in total, and all of them belong to the same species B. coli. MJ-2 and SX-1 isolates were genotype A of B. coli, and the remaining 13 isolates were genotype B. Trophozoites of MJ-2 and SX-1 were bigger, moved more slowly and lower density in feces and in vitro culture than other 13 isolates, while structures of their nuclei were not different.
  
  
  CONCLUSIONS
  Both genotype A and B of B. coli are present in the pig farms of the west of Henan province, China, and genotype B is the determinant population in pigs farms. These findings could provide an important implication for the effective control of balantidiosis of human and other hosts.
authors.affiliation: College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. wtq@mail.haust.edu.cn
authors.forename: Tianqi
authors.initials: T
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Dou
authors.forename: Wenchao
authors.initials: W
authors.lastname: Yan
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
authors.forename: Lifang
authors.initials: L
authors.lastname: Han
authors.forename: Ke
authors.initials: K
authors.lastname: Ding
authors.forename: Chuanchuan
authors.initials: C
authors.lastname: Jia
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Dujuan
authors.initials: D
authors.lastname: Yu
mesh_terms: D000818:Animals; D001447:Balantidiasis / Q000469:parasitology / Q000662:veterinary*; D001448:Balantidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S