title: Metabolic and genetic predictors of circulating adipocyte fatty acid-binding protein.
abstract: BACKGROUND
  Adipocyte fatty acid-binding protein (AFABP) was recently introduced as a novel adipokine playing an important role in glucose homeostasis. In this study, we investigated the relationship between serum AFABP levels and metabolic, as well as cardiovascular parameters, in the self-contained population of Sorbs. Furthermore, we conducted a genome-wide association study on serum AFABP concentrations in the Sorbs and we separately analyzed the effects of two common variants in the FABP4 gene on AFABP serum concentration.
  
  
  METHODS
  Serum AFABP concentrations were quantified by enzyme-linked immunosorbent assay and correlated with metabolic and cardiovascular parameters, as well as inflammatory markers and renal function, in 868 well-characterized non-diabetic Sorbs from Germany.
  
  
  RESULTS
  Median AFABP serum concentrations were 1.5-fold higher in female subjects (23.03 μg l(-1)) as compared to male subjects (15.86 μg l(-1)). Waist-to-height ratio and glomerular filtration rate were independently associated with AFABP concentrations in multiple regression analysis in both female and male subjects. The genome-wide scan for association of single-nucleotide polymorphisms with serum AFABP levels in the Sorbs revealed 39 loci reaching P-values <10(-4). Two single-nucleotide polymorphisms, rs16909187 and rs10808846, representing common genetic variation in FABP4 did not show any effect on serum AFABP concentrations in our study cohort.
  
  
  CONCLUSIONS
  AFABP serum concentrations are determined by parameters of fat distribution, renal function and gender.
authors.affiliation: Medical Department III, University of Leipzig, Liebigstrasse 18, Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Tönjes
authors.forename: S
authors.initials: S
authors.lastname: Kralisch
authors.forename: U
authors.initials: U
authors.lastname: Lössner
authors.forename: P
authors.initials: P
authors.lastname: Kovacs
authors.forename: M
authors.initials: M
authors.lastname: Blüher
authors.forename: M
authors.initials: M
authors.lastname: Stumvoll
authors.forename: M
authors.initials: M
authors.lastname: Fasshauer
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001827:Body Height; D015331:Cohort Studies; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D055106:Genome-Wide Association Study; D005858:Germany / Q000453:epidemiology; D005919:Glomerular Filtration Rate; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D051436:Renal Insufficiency, Chronic / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism*; D012307:Risk Factors; D012737:Sex Factors; D055105:Waist Circumference
chemical_list: D015415:Biomarkers; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins