title: Dynamic light scattering as an efficient tool to study glyconanoparticle-lectin interactions.
abstract: Glyconanomaterials, an emerging class of bio-functional nanomaterials, have shown promise in detecting, imaging and targeting proteins, bacteria, and cells. In this article, we report that dynamic light scattering (DLS) can be used as an efficient tool to study glyconanoparticle (GNP)--lectin interactions. Silica and Au nanoparticles (NPs) conjugated with D-mannose (Man) and D-galactose (Gal) were treated with the lectins Concanavalin A (Con A) and Ricinus communis agglutinin (RCA(120)), and the hydrodynamic volumes of the resulting aggregates were measured by DLS. The results showed that the particle size grew with increasing lectin concentration. The limit of detection (LOD) was determined to be 2.9 nM for Con A with Man-conjugated and 6.6 nM for RCA(120) with Gal-conjugated silica NPs (35 nm), respectively. The binding affinity was also determined by DLS and the results showed 3-4 orders of magnitude higher affinity of GNPs than the free ligands with lectins. The assay sensitivity and affinity were particle size dependent and decreased with increasing particle diameter. Because the method relies on the particle size growth, it is therefore general and can be applied to nanomaterials of different compositions.
authors.affiliation: Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207-0751, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Olof
authors.initials: O
authors.lastname: Ramström
authors.forename: Mingdi
authors.initials: M
authors.lastname: Yan
mesh_terms: D001386:Azides / Q000737:chemistry; D003208:Concanavalin A / Q000032:analysis; D005690:Galactose / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008027:Light; D008358:Mannose / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D037121:Plant Lectins / Q000737:chemistry; D012542:Scattering, Radiation; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D001386:Azides; D006845:Hydrocarbons, Fluorinated; D037102:Lectins; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; C556136:perfluorophenyl azide; D003208:Concanavalin A; D006046:Gold; D012822:Silicon Dioxide; D008358:Mannose; D005690:Galactose