title: Bovine serum albumin decreases Km values of human UDP-glucuronosyltransferases 1A9 and 2B7 and increases Vmax values of UGT1A9.
abstract: The human UDP-glucuronosyltransferase (UGT) enzymes UGT1A9 and UGT2B7 play important roles in the hepatic glucuronidation of many drugs. The presence of bovine serum albumin (BSA) during in vitro assays was recently reported to lower the K(m) values of both these UGTs for their aglycone substrates without affecting the corresponding V(max) values. Nonetheless, using the specific substrates entacapone and zidovudine (AZT) for UGT1A9 and UGT2B7, respectively, and using an improved ultrafiltration method for measuring drug binding to BSA and to biological membranes, we found that the presence of BSA during the glucuronidation reaction leads to a large increase in the V(max) value of UGT1A9, in addition to lowering its K(m) value. On the other hand, in the case of UGT2B7, our results agree with the previously described effect of BSA, namely lowering the K(m) value without a large effect on the enzyme's V(max) value. The unexpected BSA effect on UGT1A9 was independent of the expression system because it was found in a recombinant enzyme that was expressed in baculovirus-infected insect cells as well as in the native enzyme in human liver microsomes. Moreover, the effect of BSA on the kinetics of 4-methylumbelliferone glucuronidation by recombinant UGT1A9 was similar to its effect on entacapone glucuronidation. Contrary to the aglycone substrates, the effect of BSA on the apparent K(m) of UGT1A9 for the cosubstrate UDP-α-D-glucuronic acid was nonsignificant. Our findings call for further investigations of the BSA effects on different UGTs and the inhibitors that it may remove.
authors.affiliation: Division of Pharmaceutical Chemistry, and Centre for Drug Research, Faculty of Pharmacy, P.O. Box 56 (Viikinkaari 5), FI-00014 University of Helsinki, Finland.
authors.forename: Nenad
authors.initials: N
authors.lastname: Manevski
authors.forename: Paolo Svaluto
authors.initials: PS
authors.lastname: Moreolo
authors.forename: Jari
authors.initials: J
authors.lastname: Yli-Kauhaluoma
authors.forename: Moshe
authors.initials: M
authors.lastname: Finel
mesh_terms: D000818:Animals; D002396:Catechols / Q000378:metabolism / Q000493:pharmacokinetics; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004347:Drug Interactions; D020719:Glucuronides / Q000378:metabolism / Q000493:pharmacokinetics; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D009570:Nitriles / Q000378:metabolism / Q000493:pharmacokinetics; D011994:Recombinant Proteins / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology*; D000090264:UDP-Glucuronosyltransferase 1A9; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism; D015215:Zidovudine / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D002396:Catechols; D020719:Glucuronides; D009570:Nitriles; D011994:Recombinant Proteins; C000715591:UGT1A9 protein, human; D014535:Uridine Diphosphate Glucuronic Acid; D012710:Serum Albumin, Bovine; D006923:Hymecromone; C071192:entacapone; D015215:Zidovudine; C491474:UGT2B7 protein, human; D014453:Glucuronosyltransferase; D000090264:UDP-Glucuronosyltransferase 1A9