title: Development of a dynamic growth-death model for Escherichia coli O157:H7 in minimally processed leafy green vegetables.
abstract: Escherichia coli O157:H7, an occasional contaminant of fresh produce, can present a serious health risk in minimally processed leafy green vegetables. A good predictive model is needed for Quantitative Risk Assessment (QRA) purposes, which adequately describes the growth or die-off of this pathogen under variable temperature conditions experienced during processing, storage and shipping. Literature data on behaviour of this pathogen on fresh-cut lettuce and spinach was taken from published graphs by digitization, published tables or from personal communications. A three-phase growth function was fitted to the data from 13 studies, and a square root model for growth rate (μ) as a function of temperature was derived: μ=(0.023*(Temperature-1.20))(2). Variability in the published data was incorporated into the growth model by the use of weighted regression and the 95% prediction limits. A log-linear die-off function was fitted to the data from 13 studies, and the resulting rate constants were fitted to a shifted lognormal distribution (Mean: 0.013; Standard Deviation, 0.010; Shift, 0.001). The combined growth-death model successfully predicted pathogen behaviour under both isothermal and non-isothermal conditions when compared to new published data. By incorporating variability, the resulting model is an improvement over existing ones, and is suitable for QRA applications.
authors.affiliation: AAFC Research Associate, Central Experimental Farm, 960 Carling Ave., Ottawa, Ontario, Canada K1N0C6. Robin.McKellar@agr.gc.ca
authors.forename: Robin C
authors.initials: RC
authors.lastname: McKellar
authors.forename: Pascal
authors.initials: P
authors.lastname: Delaquis
mesh_terms: D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000254:growth & development*; D005511:Food Handling; D018545:Lactuca / Q000382:microbiology*; D008954:Models, Biological; D018515:Plant Leaves / Q000382:microbiology; D018570:Risk Assessment; D018724:Spinacia oleracea / Q000382:microbiology*; D013696:Temperature