title: Assessing G1-to-S-phase progression after genotoxic stress.
abstract: Maintenance of genomic integrity is critical for the survival of organisms. Thus, mammalian cells employ a complex DNA damage response that can sense and repair DNA damage. One important aspect of the cellular DNA damage response is the activation of checkpoints that result in cell cycle arrest. In this chapter we present methods for the induction of genotoxic stress. Additionally, we describe methods for studying the progression of cells from G(1) to S phase after genotoxic stress.
authors.affiliation: Department of Biomedical Genetics, University of Rochester Medical Center, 601 Elmwood Ave, Rochester, New York 14642, USA. michael_deran@urmc.rochester.edu
authors.forename: Michael
authors.initials: M
authors.lastname: DeRan
authors.forename: Mary
authors.initials: M
authors.lastname: Pulvino
authors.forename: Jiyong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D001761:Bleomycin / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016193:G1 Phase / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D011419:Propidium / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects* / Q000235:genetics
chemical_list: D001761:Bleomycin; D011419:Propidium; D001973:Bromodeoxyuridine