title: Association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population.
abstract: Osteoarthritis is a chronic progressive degenerative joint disease characterized by age-related regressive change in articular cartilage. A single nucleotide polymorphism has been described at position -174 of the interleukin-6 (IL-6) promoter region, leading to three possible genotypes, GG, GC, and CC. We investigated a possible association of the IL-6 -174G/C gene polymorphism with knee osteoarthritis in a Thai population. Genotype distributions and allelic frequencies of the IL-6 -174G/C polymorphism were investigated in 115 knee osteoarthritis patients and 100 healthy controls. Genotyping was performed using PCR-RFLP. The genotype distribution of IL-6 was 79 GG, 36 GC, 0 CC in knee osteoarthritis patients and 88 GG, 12 GC, 0 CC in controls. The frequency of the GC genotype in subjects with knee osteoarthritis was higher than in controls (P< 0.001). Logistic regression analysis showed that the GC genotype was independently associated with increased risk of knee osteoarthritis (odds ratio = 3.3, 95% confidence interval = 1.6-6.9, P = 0.001). These findings suggest that the -174G/C polymorphism of the IL-6 gene promoter plays a role in the pathogenesis of knee osteoarthritis.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. Sittisak.H@chula.ac.th
authors.forename: S
authors.initials: S
authors.lastname: Honsawek
authors.forename: B
authors.initials: B
authors.lastname: Deepaisarnsakul
authors.forename: A
authors.initials: A
authors.lastname: Tanavalee
authors.forename: P
authors.initials: P
authors.lastname: Yuktanandana
authors.forename: P
authors.initials: P
authors.lastname: Bumrungpanichthaworn
authors.forename: S
authors.initials: S
authors.lastname: Malila
authors.forename: N
authors.initials: N
authors.lastname: Saetan
mesh_terms: D000368:Aged; D044466:Asian People; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000208:ethnology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic
chemical_list: D015850:Interleukin-6