title: Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH-SY5Y neuroblastoma cells.
abstract: Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor-suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates.
authors.affiliation: Department of Cellular Biotechnology and Hematology, Sapienza University, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Parisi
authors.forename: Corinna
authors.initials: C
authors.lastname: Giorgi
authors.forename: Enrico Maria
authors.initials: EM
authors.lastname: Batassa
authors.forename: Laura
authors.initials: L
authors.lastname: Braccini
authors.forename: Giovanna
authors.initials: G
authors.lastname: Maresca
authors.forename: Igea
authors.initials: I
authors.lastname: D'agnano
authors.forename: Viviana
authors.initials: V
authors.lastname: Caputo
authors.forename: Annamaria
authors.initials: A
authors.lastname: Salvatore
authors.forename: Flavia
authors.initials: F
authors.lastname: Pietrolati
authors.forename: Carlo
authors.initials: C
authors.lastname: Cogoni
authors.forename: Caterina
authors.initials: C
authors.lastname: Catalanotto
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D046228:Microarray Analysis; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: C115848:AGO1 protein, human; C466422:AGO2 protein, human; D060565:Argonaute Proteins; D039642:Eukaryotic Initiation Factors; D016159:Tumor Suppressor Protein p53