title: Protein profiling of human nonpigmented ciliary epithelium cell secretome: the differentiation factors characterization for retinal ganglion cell line.
abstract: The purpose of this paper was to characterize proteins secreted from the human nonpigmented ciliary epithelial (HNPE) cells, which have differentiated a rat retinal ganglion cell line, RGC-5. Undifferentiated RGC-5 cells have been shown to express several marker proteins characteristic of retinal ganglion cells. However, RGC-5 cells do not respond to N-methyl-D aspartate (NMDA), or glutamate. HNPE cells have been shown to secrete numbers of neuropeptides or neuroproteins also found in the aqueous humor, many of which have the ability to influence the activity of neuronal cells. This paper details the profile of HNPE cell-secreted proteins by proteomic approaches. The experimental results revealed the identification of 132 unique proteins from the HNPE cell-conditioned SF-medium. The biological functions of a portion of these identified proteins are involved in cell differentiation. We hypothesized that a differentiation system of HNPE cell-conditioned SF-medium with RGC-5 cells can induce a differentiated phenotype in RGC-5 cells, with functional characteristics that more closely resemble primary cultures of rat retinal ganglion cells. These proteins may replace harsh chemicals, which are currently used to induce cell differentiation.
authors.affiliation: Department of Chemical and Material Engineering, National Yunlin University of Science and Technology, Douliou, Yunlin, Taiwan.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.forename: Raghu R
authors.initials: RR
authors.lastname: Krishnamoorthy
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chu
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Yang
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.forename: Sharon Chia-Ju
authors.initials: SC
authors.lastname: Chen
authors.forename: Adnan
authors.initials: A
authors.lastname: Dibas
authors.forename: Thomas
authors.initials: T
authors.lastname: Yorio
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D017077:Culture Media, Conditioned; D030562:Databases, Protein; D004847:Epithelial Cells / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000145:classification / Q000378:metabolism*; D010641:Phenotype; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D053719:Tandem Mass Spectrometry
chemical_list: D017077:Culture Media, Conditioned; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D020543:Proteome