title: Free sphingobases induce RBOHD-dependent reactive oxygen species production in Arabidopsis leaves.
abstract: Sphingolipids are implied in several regulatory processes, including cell death. Levels of the free sphingobase t18:0 (phytosphingosine) increase in Arabidopsis in response to the bacterial pathogen Pseudomonas syringae. To gain information on sphingobase-induced signaling, we determined kinetics of leaf reactive oxygen species (ROS) levels and cell death in response to specific sphingobases. t18:0, d18:0 and d17:1, but not d20:0, induced ROS and cell death within 1.5-2h. Early sphingobase-induced ROS production was independent of cell death induction and required the NADPH oxidase Respiratory Burst Oxidase Homolog D (RBOHD). Specific sphingobases can therefore induce cell death and require RBOHD for early ROS induction in plants.
authors.affiliation: Julius-von-Sachs Institute of Biosciences, Pharmaceutical Biology, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Peer
authors.forename: Matthias
authors.initials: M
authors.lastname: Bach
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Mueller
authors.forename: Frank
authors.initials: F
authors.lastname: Waller
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology* / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D044224:Pseudomonas syringae / Q000378:metabolism / Q000472:pathogenicity; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013107:Sphingolipids / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D007527:Isoenzymes; D017382:Reactive Oxygen Species; D013107:Sphingolipids; C568857:AT5G47910 protein, Arabidopsis; D019255:NADPH Oxidases; C022709:superoxide-forming enzyme