title: [Influence of cytokine combinations on proliferation and differentiation of umbilical cord blood CD34(+) cells into megakaryocytes/platelets in vitro].
abstract: In order to investigate the influence of cytokine combinations on proliferation and differentiation of human umbilical cord blood CD34(+) cells into megakaryocytes/platelets in vitro, the CD34(+) cells from human umbilical cord blood were amplified in serum-free medium StemSpan(SFEM) supplemented with several cytokine combinations by three-phase culture system. The effects of the cytokine combinations were compared. The results showed that at day 14 of the first culture phase, the CD34(+) cells cultured with cytokine combinations SCF + TPO + FL + IL-3 were amplified (11 000 ± 1 000) times, which were significantly higher than that of cells cultured with SCF + TPO + FL, but were not significantly different from that of cells cultured with SCF + TPO + IL-3 or SCF + TPO + FL + IL-3+ hydroxyl-corticosteroids. At day 7 of the second culture phase, the CD34(+) cells cultured with cytokine combination SCF + TPO + FL + IL-11 were amplified by (204666.7 ± 11718.9) times, which were significantly higher than that of cells cultured with SCF + TPO + FL + IL-3, but were not significantly different from that of cells cultured with SCF + TPO + FL + IL-11 + BMP4 + VEGF. At day 3 and day 6, the CD34(+) platelet-like cells accounted for about (39.8 ± 1.9)%, (39.7 ± 2.6)% and (25.5 ± 1.4)%, (23.1 ± 3.5)% cultured with SCF + TPO + FL + IL-11 and SCF + TPO + FL + IL-11 + BMP4 + VEGF, and significantly higher than that of the cells cultured with SCF + TPO + FL + IL-3. It is concluded that the cytokine combination of SCF + TPO + FL + IL-3 is most suitable cytokines combination for the amplification of CD34(+) hematopoietic progenitor cells. The cytokine combination of SCF + TPO + FL + IL-11 is preferred for the proliferation and differentiation of megakaryocytes, this study lays an experimental basis for investigating the proliferation and differentiation of CD34(+) into megakaryocytes/platelets in vitro.
authors.affiliation: Beijing Red Cross Blood Service Center, Beijing, China.
authors.forename: Ke-Ying
authors.initials: KY
authors.lastname: Zhang
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Duan
authors.forename: Song-Ming
authors.initials: SM
authors.lastname: Gao
authors.forename: Shuang
authors.initials: S
authors.lastname: Cui
authors.forename: Zhi-Yin
authors.initials: ZY
authors.lastname: Gong
authors.forename: Lei
authors.initials: L
authors.lastname: Ni
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Zhang
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology; D001792:Blood Platelets / Q000166:cytology*; D002454:Cell Differentiation; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006801:Humans; D017370:Interleukin-11 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D008533:Megakaryocytes / Q000166:cytology*; D019089:Stem Cell Factor / Q000494:pharmacology; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: D018952:Antigens, CD34; D017370:Interleukin-11; D007377:Interleukin-3; D019089:Stem Cell Factor; D013926:Thrombopoietin