title: Phosphine-free synthesis of high-quality reverse type-I ZnSe/CdSe core with CdS/Cd(x)Zn(1 - x)S/ZnS multishell nanocrystals and their application for detection of human hepatitis B surface antigen.
abstract: Highly photoluminescent (PL) reverse type-I ZnSe/CdSe nanocrystals (NCs) and ZnSe/CdSe/CdS/Cd(x)Zn(1 - x)S/ZnS core/multishell NCs were successfully synthesized by a phosphine-free method. By this low-cost, 'green' synthesis route, more than 10 g of high-quality ZnSe/CdSe/CdS/Cd(x)Zn(1 - x)S/ZnS NCs were synthesized in a large scale synthesis. After the overgrowth of a CdS/Cd(x)Zn(1 - x)S/ZnS multishell on ZnSe/CdSe cores, the PL quantum yields (QYs) increased from 28% to 75% along with the stability improvement. An amphiphilic oligomer was used as a surface coating agent to conduct a phase transfer experiment, core/multishell NCs were dissolved in water by such surface modification and the QYs were still kept above 70%. The as-prepared water dispersible ZnSe/CdSe/CdS/Cd(x)Zn(1 - x)S/ZnS core/multishell NCs not only have high fluorescence QYs but also are extremely stable in various physiological conditions. Furthermore, a biosensor system (lateral flow immunoassay system, LFIA) for the detection of human hepatitis B surface antigen (HBsAg) was developed by using this water-soluble core/multishell NCs as a fluorescent label and a nitrocellulose filter membrane for lateral flow. The result showed that such ZnSe/CdSe/CdS/Cd(x)Zn(1 - x)S/ZnS core/multishell NCs were excellent fluorescent labels to detect HBsAg. The sensitivity of HBsAg detection could reach as high as 0.05 ng ml( - 1).
authors.affiliation: Key Laboratory for Special Functional Materials, Henan University, Kaifeng 475004, People's Republic of China.
authors.forename: Huaibin
authors.initials: H
authors.lastname: Shen
authors.forename: Hang
authors.initials: H
authors.lastname: Yuan
authors.forename: Jin Zhong
authors.initials: JZ
authors.lastname: Niu
authors.forename: Shasha
authors.initials: S
authors.lastname: Xu
authors.forename: Changhua
authors.initials: C
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Ma
authors.forename: Lin Song
authors.initials: LS
authors.lastname: Li
mesh_terms: D002021:Buffers; D019187:Cadmium Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010720:Phosphines / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D012666:Semiconductors; D012995:Solubility; D013057:Spectrum Analysis; D013440:Sulfides / Q000138:chemical synthesis* / Q000737:chemistry; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D002021:Buffers; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D006514:Hepatitis B Surface Antigens; D010720:Phosphines; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C034939:cadmium sulfide; D014867:Water; C058667:cadmium selenide; C044646:phosphine; C031238:zinc sulfide; C044696:zinc selenide