title: [On-line detection of hydrocortisone concentration in rat subcutaneous adipose with liquid chromatography-mass spectrometry/mass spectrometry].
abstract: OBJECTIVE
  To develop a method for detecting the topical concentration of hydrocortisone (HC) in the subcutaneous adipose of rats using microdialysis sampling technique and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS).
  
  
  METHODS
  Topical samples were collected by applying the probe into the subcutaneous adipose of rats, with alcohol (5%)-ringers solution as the perfusion solution. A LC-MS/MS method was established for detecting the HC concentration in the dialysates.
  
  
  RESULTS
  The protonated precursor to produce ion transitions monitored for HC was m/z 363.2→121.1. The calibration curve was linear over the range of 0.5-1000 ng/ml, with the intra- and inter-day precision and accuracy within ∓15%, and no significant matrix effect was noted. The in vivo recovery of the probe was about 59%.
  
  
  CONCLUSIONS
  A selective and sensitive method has been successfully established for the on-line HC detection in the subcutaneous adipose of rats.
authors.affiliation: Department of Pharmacy, Zijin Peoples Hospital, Heyuan 517400, China. 279433464@qq.com
authors.forename: Li-zhen
authors.initials: LZ
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Lu-qi
authors.initials: LQ
authors.lastname: Xiong
authors.forename: Guo-feng
authors.initials: GF
authors.lastname: Li
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D006854:Hydrocortisone / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D050151:Subcutaneous Fat / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D006854:Hydrocortisone