title: The influence of elasticity and surface roughness on myogenic and osteogenic-differentiation of cells on silk-elastin biomaterials.
abstract: The interactions of C2C12 myoblasts and human bone marrow stem cells (hMSCs) with silk-tropoelastin biomaterials, and the capacity of each to promote attachment, proliferation, and either myogenic- or osteogenic-differentiation were investigated. Temperature-controlled water vapor annealing was used to control beta-sheet crystal formation to generate insoluble silk-tropoelastin biomaterial matrices at defined ratios of the two proteins. These ratios controlled surface roughness and micro/nano-scale topological patterns, and elastic modulus, stiffness, yield stress, and tensile strength. A combination of low surface roughness and high stiffness in the silk-tropoelastin materials promoted proliferation and myogenic-differentiation of C2C12 cells. In contrast, high surface roughness with micro/nano-scale surface patterns was favored by hMSCs. Increasing the content of human tropoelastin in the silk-tropoelastin materials enhanced the proliferation and osteogenic-differentiation of hMSCs. We conclude that the silk-tropoelastin composition facilitates fine tuning of the growth and differentiation of these cells.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.forename: Sang-Hyug
authors.initials: SH
authors.lastname: Park
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Gil
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Xia
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
mesh_terms: D000328:Adult; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D004548:Elasticity; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008297:Male; D032446:Myoblasts / Q000166:cytology*; D010012:Osteogenesis; D047011:Silk / Q000737:chemistry*; D013499:Surface Properties; D014333:Tropoelastin / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D047011:Silk; D014333:Tropoelastin