title: miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells.
abstract: OBJECTIVE
  To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC).
  
  
  METHODS
  The expression of miR-149 was examined by real-time PCR and calculated by 2(-▵▵Ct) method. The cell proliferation was analyzed by MTT assay. The cell migration and invasion were shown by the wound healing assay and transwell migration assay, and the expression of E-cadherin was detected by Western blot.
  
  
  RESULTS
  The expression of miR-149 was higher in NPC cell lines 5-8F and 6-10B than that in normal immortalized nasopharyngeal epithelial NP69. MTT assay showed that miR-149 promoted the proliferation of NPC cell lines. The wound healing assay showed miR-149 promoted the mobility and invasion of NPC cell lines. Inhibition of miR-149 reduced the ability of NPC cell lines to proliferate and invade. miR-149 downregulated the expression of E-cadherin, whereas antagomir which mediated knockdown of miR-149 significantly upregulated the expression of E-cadherin.
  
  
  CONCLUSIONS
  miR-149 might be involved in the invasion and metastasis of NPC through regulation of epithelial-mesenchymal transition (EMT).
authors.affiliation: Cancer Research Institute, Central South University, Changsha 410078, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Luo
authors.forename: Liyang
authors.initials: L
authors.lastname: Zhang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
authors.forename: Yafei
authors.initials: Y
authors.lastname: Dai
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Haibo
authors.initials: H
authors.lastname: Yu
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xiang
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D009361:Neoplasm Invasiveness
chemical_list: D015820:Cadherins; C551557:MIRN149 microRNA, human; D035683:MicroRNAs