title: Altered arachidonate distribution in macrophages from caveolin-1 null mice leading to reduced eicosanoid synthesis.
abstract: In this work we have studied the effect of caveolin-1 deficiency on the mechanisms that regulate free arachidonic acid (AA) availability. The results presented here demonstrate that macrophages from caveolin-1-deficient mice exhibit elevated fatty acid incorporation and remodeling and a constitutively increased CoA-independent transacylase activity. Mass spectrometry-based lipidomic analyses reveal stable alterations in the profile of AA distribution among phospholipids, manifested by reduced levels of AA in choline glycerophospholipids but elevated levels in ethanolamine glycerophospholipids and phosphatidylinositol. Furthermore, macrophages from caveolin-1 null mice show decreased AA mobilization and prostaglandin E(2) and LTB(4) production upon cell stimulation. Collectively, these results provide insight into the role of caveolin-1 in AA homeostasis and suggest an important role for this protein in the eicosanoid biosynthetic response.
authors.affiliation: Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas, 47003 Valladolid, Spain.
authors.forename: Alma M
authors.initials: AM
authors.lastname: Astudillo
authors.forename: Gema
authors.initials: G
authors.lastname: Pérez-Chacón
authors.forename: Clara
authors.initials: C
authors.lastname: Meana
authors.forename: David
authors.initials: D
authors.lastname: Balgoma
authors.forename: Albert
authors.initials: A
authors.lastname: Pol
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Del Pozo
authors.forename: María A
authors.initials: MA
authors.lastname: Balboa
authors.forename: Jesús
authors.initials: J
authors.lastname: Balsinde
mesh_terms: D000217:Acyltransferases; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D051242:Caveolin 1 / Q000378:metabolism*; D002478:Cells, Cultured; D015777:Eicosanoids / Q000378:metabolism*; D004952:Esters / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007249:Inflammation; D008055:Lipids / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051242:Caveolin 1; D015777:Eicosanoids; D004952:Esters; D008055:Lipids; D010743:Phospholipids; D016718:Arachidonic Acid; D000217:Acyltransferases; C039766:arachidonyl transacylase