title: Isolation, characterization of Rhodococcus sp. P14 capable of degrading high-molecular-weight polycyclic aromatic hydrocarbons and aliphatic hydrocarbons.
abstract: Rhodococcus sp. P14 was isolated from crude oil-contaminated sediments. This strain was capable of utilizing three to five rings polycyclic aromatic hydrocarbons (PAHs) including phenanthrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) as a sole carbon and energy source. After cultivated with 50mg/L of each PAH, strain P14 removed 43% Phe, 34% Pyr and 30% BaP in 30 d. Four different hydroxyphenanthrene products derived from Phe by strain P14 (1,2,3,4-hydroxyphenanthrene) were detected using SPME-GC-MS. Strain P14 also was capable of degrading mineral oil with n-alkanes of C17 to C21 carbon chain length. Compared with glucose-grown cells, PAHs-grown cells had decreased contents of shorter-chain length fatty acids (≤ C16:0), increased contents of C18:0, Me-C19:0 and disappeared odd-number carbon chain fatty acids. The contents of unsaturated C19:1, Me-C19:0 increased and C18:0 decreased in mineral oil-grown cells. At the same time, the strain P14 tended to float when cultivated in mineral oil-supplemented liquid medium. The degradation capability of P14 to alkane and PAHs and its floating characteristics will be very helpful for future's application in oil-spill bioremediation.
authors.affiliation: Department of Biology, Shantou University, Shantou 515063, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Song
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Gangmin
authors.initials: G
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Tongwang
authors.initials: T
authors.lastname: Huang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Hu
mesh_terms: D000473:Alkanes / Q000378:metabolism; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology; D008969:Molecular Sequence Data; D061329:Oil and Gas Fields / Q000382:microbiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012240:Rhodococcus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000473:Alkanes; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical