title: Efficacy of vitamin E and selenium for the prevention of intra-abdominal adhesions in rats: uterine horn models.
abstract: OBJECTIVE
  This study compares the efficacies of vitamin E and selenium, both individually and in combination, for the prevention of postoperative intra-abdominal adhesions in rats.
  
  
  METHODS
  Forty-seven female rats were divided into five groups. The sham animals (S group, n = 7) were given only laparotomies and intraperitoneally received 0.9% NaCl (2 ml). In the 40 other rats, abrasions of the left uterine horn were performed, followed by intraperitoneal administration of either 2 ml 0.9% NaCl (C group), 10 mg vitamin E (vitamin E group), 0.2 mg/kg selenium (Se group) or 10 mg vitamin E with 0.2 mg/kg selenium (vitamin E + Se group), with 10 animals in each treatment group.
  
  
  RESULTS
  Adhesion formation was significantly reduced in animals in the Se and vitamin E + Se groups (p<0.05). Tissue catalase and glutathione peroxidase activities did not significantly differ between the groups. However, catalase and glutathione peroxidase activities and reduced glutathione levels were slightly increased in the vitamin E, Se and vitamin E + Se groups. In the vitamin E group, malondialdehyde concentrations were significantly lower than in the C group (p<0.05), but no significant differences were present among the S, C, Se and vitamin E + Se groups. Levels of nitric oxide were significantly higher in the C group than in the other groups (p<0.01).
  
  
  CONCLUSIONS
  Intraperitoneal administration of selenium or combined vitamin E and selenium appears to be effective in preventing intra-abdominal adhesion formation in rat models through the reduction of lipid peroxidation products.
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey. asdurmus@firat.edu.tr
authors.forename: Ali Said
authors.initials: AS
authors.lastname: Durmus
authors.forename: Hamit
authors.initials: H
authors.lastname: Yildiz
authors.forename: Ihsan
authors.initials: I
authors.lastname: Yaman
authors.forename: Halil
authors.initials: H
authors.lastname: Simsek
mesh_terms: D000005:Abdomen; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D002374:Catalase / Q000032:analysis; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000627:therapeutic use*; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control; D014591:Uterine Diseases / Q000473:pathology*; D014810:Vitamin E / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D012643:Selenium