title: Notification of the first isolation of Cacipacore virus in a human in the State of Rondônia, Brazil.
abstract: Flavivirus is a genus of arthropod-transmitted viruses of the family Flaviviridae, and in Brazil, up to eleven different Flavivirus have been isolated. We collected blood from farmers in the municipality of Theobroma, which is located 320km from the City of Porto Velho, the former capital of the Brazilian State of Rondônia. For viral isolation, we used newborn mouse brain, followed by RT-PCR with specific universal Flavivirus primers. We obtained fragments 958bp and 800bp in length. Based on BLAST, these sequences were 91% similar to a sequence of Cacipacore virus.
authors.affiliation: Laboratório de Virologia, Instituto de Pesquisas em Patologias Tropicais, Porto Velho, RO, Brasil. weber_cepem@hotmail.com
authors.forename: Weber Cheli
authors.initials: WC
authors.lastname: Batista
authors.forename: Glauciane da Silva Bifano
authors.initials: Gda S
authors.lastname: Tavares
authors.forename: Deusilene Souza
authors.initials: DS
authors.lastname: Vieira
authors.forename: Eduardo Rezende
authors.initials: ER
authors.lastname: Honda
authors.forename: Soraya Santos
authors.initials: SS
authors.lastname: Pereira
authors.forename: Mauro Shugiro
authors.initials: MS
authors.lastname: Tada
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D005416:Flavivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018177:Flavivirus Infections / Q000453:epidemiology / Q000821:virology*; D006801:Humans; D008297:Male; D051379:Mice; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral