title: Shotgun proteomics and network analysis of ubiquitin-related proteins from human breast carcinoma epithelial cells.
abstract: Protein ubiquitination via the covalent attachment of ubiquitin (Ub) plays an important role in the regulation of the stability, function or localization of multiple proteins in eukaryotic cells. Comprehensive investigation of the proteomics related to ubiquitination will gain the insight into the Ub-mediated regulatory mechanism. In the present study, the combination of polyUb affinity purification, SDS-PAGE separation, and liquid chromatography-tandem mass spectrometry analysis (GeLC-MS/MS) was employed to analyze the Ub-related proteins in human MDA-MB-231 breast carcinoma epithelial cells after treatment with the proteasome inhibitor MG132. A total of 260 non-redundant Ub-related proteins were identified from the cells. These proteins were shown to be involved in a host of critical cellular functions and processes, including transcription, translation, Ub-proteasome pathway, cell cycle, heat shock response, transport, etc. The interaction network analysis by STRING indicated that the identified Ub-related proteins formed eleven clusters, the three most highly ranked network clusters were mainly involved in protein translation, RNA transcription and processing, and Ub-proteasome pathway, suggesting that there were obvious ubiquitination-mediated alternations in gene expression of human MDA-MB-231 cells. The proteomic profiling and their interaction network analysis in this study would help to our systematic understanding of the Ub-related cellular protein functions and the related biological processes in human disease tissue cells.
authors.affiliation: The Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, College of Life Sciences, Hunan Normal University, Changsha City, 410081, Hunan, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Deng
authors.initials: D
authors.lastname: Bi
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Xianchun
authors.initials: X
authors.lastname: Wang
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000737:chemistry* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011480:Protease Inhibitors; D060066:Protein Interaction Maps; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D054875:Ubiquitination; D014452:Ubiquitins / Q000032:analysis*
chemical_list: D011480:Protease Inhibitors; D011506:Proteins; D014452:Ubiquitins