title: Effects of TiO2-containing phosphate glasses on solubility and in vitro biocompatibility.
abstract: Phosphate-based glasses with different amounts of titanium dioxide (TiO(2)), having the following molar composition 50P(2)O(5)-30CaO-9Na(2)O-3SiO(2)-3MgO-(5-x)K(2)O-xTiO(2), (where x = 0, 2.5, 5 mol %), were synthesised and characterized in terms of solubility (according to ISO 10993-14), and in vitro biocompatibility using human MG-63 osteoblast cells. Dissolution tests were carried out in Tris-HCl (pH 7.4) to simulate the physiological pH and in citric acid (pH 3.0) to simulate an acidic environment. The weight loss decreased with increasing TiO(2) content, a process further enhanced in acidic medium. TiO(2) reduced the pH changes usually caused by the dissolution products released. Cellular tests showed that all the glasses studied (0-5 mol % TiO(2)) and TiCl(4), used to investigate the biocompatibility of titanium ions, did not produce cytotoxic effects on human MG-63 osteoblasts for up to 5 days in culture. On the basis of these results, we suggest that TiO(2)-containing phosphate glasses could be promising substrates for bone tissue engineering applications.
authors.affiliation: Materials Science and Chemical Engineering Department, Politecnico di Torino, Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Novajra
authors.forename: C
authors.initials: C
authors.lastname: Vitale-Brovarone
authors.forename: J C
authors.initials: JC
authors.lastname: Knowles
authors.forename: G
authors.initials: G
authors.lastname: Maina
authors.forename: V
authors.initials: V
authors.lastname: Aina
authors.forename: D
authors.initials: D
authors.lastname: Ghigo
authors.forename: L
authors.initials: L
authors.lastname: Bergandi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D010710:Phosphates / Q000737:chemistry* / Q000494:pharmacology; D012995:Solubility; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D010710:Phosphates; C009495:titanium dioxide; D014025:Titanium