title: The effect of subretinal viscoelastics on the porcine retinal function.
abstract: The functional consequence of long-term retinal detachment in the porcine model is examined by multifocal electroretinography (mfERG). Retinal detachment (RD) in humans leaves permanent visual impairment, despite anatomical successful reattachment surgery. To improve treatment, adjuvant pharmaceutical therapy is needed, and can only be tested in a suitable animal model. The porcine model is promising and the mfERG is well validated in this model. RD was induced in 18 pigs by vitrectomy and healon injection of various concentrations. Preoperatively and 6 weeks postoperatively eight animals were examined by mfERG. The major component P1 was analyzed statistically. Indirect ophthalmoscopy and bilateral color fundus photography (FP) were performed. Selected animals underwent high-resolution optical coherence tomography (OCT). Examination by ophthalmoscopy and FP showed that the RDs remained detached for the 6 weeks of follow-up. The P1 amplitude of the mfERG did not differ significantly between the detached areas, the surrounding attached areas, and the healthy eye (p = 0.25). Similarly, P1 implicit time did not differ between the areas (p = 0.85). The lack of functional consequences of long-term RD makes the porcine model unsuitable for examining adjuvant pharmaceutical RD treatment. Future studies should focus on foveated primates.
authors.affiliation: Department of Ophthalmology, Glostrup Hospital, University of Copenhagen, Copenhagen, Denmark. nina.sorensen@dadlnet.dk
authors.forename: Nina Fischer
authors.initials: NF
authors.lastname: Sørensen
authors.forename: Rasmus
authors.initials: R
authors.lastname: Ejstrup
authors.forename: Thøger Frøsig
authors.initials: TF
authors.lastname: Svahn
authors.forename: Birgit
authors.initials: B
authors.lastname: Sander
authors.forename: Jens
authors.initials: J
authors.lastname: Kiilgaard
authors.forename: Morten
authors.initials: M
authors.lastname: la Cour
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004596:Electroretinography / Q000187:drug effects*; D058450:Endotamponade; D005260:Female; D006820:Hyaluronic Acid / Q000633:toxicity*; D009887:Ophthalmoscopy; D012075:Remission, Spontaneous; D012160:Retina / Q000187:drug effects / Q000502:physiology*; D012163:Retinal Detachment / Q000175:diagnosis / Q000503:physiopathology* / Q000601:surgery; D034421:Sus scrofa; D041623:Tomography, Optical Coherence; D055675:Viscosupplements / Q000633:toxicity*; D014821:Vitrectomy
chemical_list: D055675:Viscosupplements; D006820:Hyaluronic Acid