title: [The affect of Si-Rac1b on the malignant biological behaviors of colorectal cancer cell].
abstract: OBJECTIVE
  To observe the affect of Small interfering RNA Rac1b (Si-Rac1b) on the malignant biological behaviors of colorectal cancer cell including the proliferation, migration, invasion and apoptosis of the cells.
  
  
  METHODS
  Mediated by lipofectamine 2000, Si-Rac1b was transfected into colorectal cancer cell line SW1116 (with overexpression of Rac1b). The expression of Rac1b was detected by Western blotting and RT-PCR. The CCK-8 assay was used to analyze the cell proliferation, the Wound-healing assay and invasion assay were respectively applied to analyze the cell migration and invasion, and the Hoechst 33258 was used to evaluate the apoptotic index.
  
  
  RESULTS
  Si-Rac1b can knock down the Rac1b but not Rac1 both in the level of mRNA and protein. In addition, Si-Rac1b could singnificantly facilitate the cell proliferation, migration, invasion and control the cell apoptosis.
  
  
  CONCLUSIONS
  Si-Rac1b could partically reverse the malignant phenotypes of colorectal cancer cell.
authors.affiliation: Department of Abdominal Cancer, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li-Lan
authors.initials: LL
authors.lastname: Li
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Yue
authors.forename: Nan-Jing
authors.initials: NJ
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Wei
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Tang
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection; D020830:rac1 GTP-Binding Protein / Q000235:genetics*
chemical_list: C107953:RAC1 protein, human; D034741:RNA, Small Interfering; D020830:rac1 GTP-Binding Protein