title: Analysis of aflatoxin-B1-degrading microbes by use of a combined toxicity-profiling method.
abstract: To monitor cytotoxic and genotoxic effects of aflatoxin, a luminescent assay employing Aliivibrio fischeri as a test organism and a colorimetric assay based on the SOS-Chromotest were adapted to our needs. The aim of this method-developing work was to be able to select - from a collection of environmental isolates - microbes that degrade aflatoxin without production of harmful intermediates and by-products, in a fast and cost-effective way. By the combination of the two modified assays, microbes that met these criteria have been successfully selected. Among thirty-three isolates, the strain Rhodococcus rhodochrous NI2 proved to be the best aflatoxin-B1-degrading microbe, with the weakest harmful biological effects throughout aflatoxin-B1-degradation. Our findings underline the necessity to employ bio-tests in biodegradation assays, as cytotoxicity and/or genotoxicity may occur even after substantial degradation of the toxins.
authors.affiliation: Department of Environmental Protection and Safety, Szent István University, Gödöllő, Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: Krifaton
authors.forename: Balázs
authors.initials: B
authors.lastname: Kriszt
authors.forename: Sándor
authors.initials: S
authors.lastname: Szoboszlay
authors.forename: Mátyás
authors.initials: M
authors.lastname: Cserháti
authors.forename: Adám
authors.initials: A
authors.lastname: Szucs
authors.forename: József
authors.initials: J
authors.lastname: Kukolya
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism*; D059706:Aliivibrio / Q000378:metabolism; D001673:Biodegradation, Environmental; D009152:Mutagenicity Tests / Q000379:methods; D012240:Rhodococcus / Q000378:metabolism; D018675:Toxicity Tests / Q000379:methods
chemical_list: D016604:Aflatoxin B1