title: PCL film surfaces conjugated with P(DMAEMA)/gelatin complexes for improving cell immobilization and gene transfection.
abstract: Successful gene transfection on a tissue scaffold is of crucial importance in facilitating tissue repair and regeneration by enabling the localized production of therapeutic drugs. Polycaprolactone (PCL) has been widely adopted as a scaffold biomaterial, but its unfavorable cell-adhesion property needs to be improved. In this work, the PCL film surface was conjugated with poly((2-dimethyl amino)ethyl methacrylate) (P(DMAEMA))/gelatin complexes via surface-initiated atom transfer radical polymerization (ATRP) for improving cell immobilization and subsequent gene transfection. A simple aminolysis-based method was first used for the covalent immobilization of ATRP initiators on the PCL film. Well-defined P(DMAEMA) brushes were subsequently prepared via surface-initiated ATRP from the initiator-functionalized PCL surfaces. The P(DMAEMA) chains with a pK(a) of 7.0-7.3 were used for conjugating gelatin with a pI of 4.7 via electrostatic interaction. The amount of complexed gelatin increased as that of the grafted P(DMAEMA) layer. The cell-adhesion property on the functionalized PCL surface could be controlled by adjusting the ratio of P(DMAEMA)/gelatin. It was found that the gene transfection property on the immobilized cells was dependent on the density of the immobilized cells on the functionalized PCL film. With the good cell-adhesive nature of gelatin and the efficient gene transfection on the dense immobilized cells, the incorporating the suitable of P(DMAEMA)/gelatin complexes onto PCL surfaces could endow the PCL substrates new and interesting properties for potential tissue engineering applications.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Yuan
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: J S
authors.initials: JS
authors.lastname: Li
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
authors.forename: W T
authors.initials: WT
authors.lastname: Yang
authors.forename: J
authors.initials: J
authors.lastname: Ma
mesh_terms: D002448:Cell Adhesion; D002460:Cell Line; D018914:Cells, Immobilized; D005434:Flow Cytometry; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D009757:Nylons / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D058105:Polymerization; D055672:Static Electricity; D013499:Surface Properties; D014162:Transfection / Q000379:methods*
chemical_list: D008689:Methacrylates; D009757:Nylons; D011091:Polyesters; C109846:poly(2-(diethylamino)ethyl methacrylate); C016240:polycaprolactone; D005780:Gelatin