title: Shotgun isotope array for rapid, substrate-specific detection of microorganisms in a microbial community.
abstract: The shotgun isotope array method has been proposed to be an effective new tool for use in substrate-specific microbe exploration without any prior knowledge of the community composition. Proof of concept was demonstrated by detection of acetate-degrading microorganisms in activated sludge and further verified by independent stable isotope probing (SIP).
authors.affiliation: Department of Urban Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. tobino@env.t.u-tokyo.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Tobino
authors.forename: Futoshi
authors.initials: F
authors.lastname: Kurisu
authors.forename: Ikuro
authors.initials: I
authors.lastname: Kasuga
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Furumai
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007554:Isotopes / Q000378:metabolism*; D008969:Molecular Sequence Data; D010961:Plasmodium / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D007554:Isotopes; D012336:RNA, Ribosomal, 16S; D012722:Sewage