title: Deep, subsurface microflora after excavation respiration and biomass and its potential role in degradation of fossil organic matter.
abstract: Three types of Miocene claystones (amorphous, lamellar, and transitional) were aseptically sampled from depths of 30 m and 150 m below the soil surface. Respiration of these sediments was measured under conditions that prevented inoculation by other microorganisms not indigenous to the claystones in situ. Microbial respiration was higher in lamellar than amorphous claystones and was not affected by sampling depth. During cultivation, microbial biomass (as indicated by PLFA) significantly increased. Microbial biomass after cultivation was significantly higher in sediments from 30 m than from 150 m depth. Both microbial respiration and biomass increased after glucose addition.
authors.affiliation: Institute of Soil Biology BC ASCR, Na Sádkách 7, CZ37005, České Budějovice, Czech Republic. frouz@natur.cuni.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Frouz
authors.forename: Tomas
authors.initials: T
authors.lastname: Cajthaml
authors.forename: Bohdan
authors.initials: B
authors.lastname: Kříbek
authors.forename: Philipe
authors.initials: P
authors.lastname: Schaeffer
authors.forename: Martin
authors.initials: M
authors.lastname: Bartuška
authors.forename: Renata
authors.initials: R
authors.lastname: Galertová
authors.forename: Petr
authors.initials: P
authors.lastname: Rojík
authors.forename: Vaclav
authors.initials: V
authors.lastname: Krištůfek
mesh_terms: D000332:Aerobiosis; D000538:Aluminum Silicates / Q000032:analysis* / Q000145:classification; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D000077215:Clay; D005227:Fatty Acids / Q000032:analysis; D005580:Fossils; D005658:Fungi / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000378:metabolism; D059013:Microbial Consortia / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D052616:Solid Phase Extraction
chemical_list: D000538:Aluminum Silicates; D005227:Fatty Acids; D010743:Phospholipids; D012987:Soil; D005947:Glucose; D010100:Oxygen; D000077215:Clay