title: Generation of recombinant antibody fragments that target canine dendritic cells by phage display technology.
abstract: One of the main goals in cancer immunotherapy is the efficient activation of the host immune system against tumour cells. Dendritic cells (DCs) can induce specific anti-tumour immune responses in both experimental animal models and humans. However, most preclinical studies using small animal models show only limited correlation with studies carried out in clinical settings, whereas laboratory dogs naturally develop tumours that are biologically and histopathologically similar to their human counterparts. Here, we describe the generation and characterization of recombinant antibodies against canine DCs, isolated using the Tomlinson phage display system. We successfully isolated highly specific single-chain variable fragment (scFv) antibodies in a sequential three-step panning strategy involving depletion on canine peripheral blood mononuclear cells followed by positive selection on native canine DCs. This provides the basis for an antibody-based method for the immunological detection and manipulation of DCs and for monitoring antigen-specific immune responses.
authors.affiliation: Experimental Medicine and Immunotherapy, Department of Applied Medical Engineering, Helmholtz Institute for Biomedical Engineering, University Hospital RWTH Aachen, Aachen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Fitting
authors.forename: D
authors.initials: D
authors.lastname: Killian
authors.forename: C
authors.initials: C
authors.lastname: Junghanss
authors.forename: S
authors.initials: S
authors.lastname: Willenbrock
authors.forename: H
authors.initials: H
authors.lastname: Murua Escobar
authors.forename: S
authors.initials: S
authors.lastname: Lange
authors.forename: I
authors.initials: I
authors.lastname: Nolte
authors.forename: S
authors.initials: S
authors.lastname: Barth
authors.forename: M K
authors.initials: MK
authors.lastname: Tur
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D018952:Antigens, CD34 / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D004285:Dogs; D005260:Female; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D019151:Peptide Library; D011994:Recombinant Proteins / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000276:immunology
chemical_list: D018952:Antigens, CD34; D019151:Peptide Library; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies