title: Effects of immune reaction in rats after acute carbon monoxide poisoning.
abstract: This study is designed to observe the immune reaction in rats after acute carbon monoxide (CO) poisoning. We observed brain injury, cognitive impairment, a variety of microglias and expression of immune factors, including major histocompatibility complex II (MHCII), CD4, vascular cell adhesion molecule-1 (VCAM-1) and interferon-gamma (IFN-gamma) in the brain tissues of CO-poisoned rats. Then relationships between cognitive impairment and immune factors were explored. We found that there were extensive neuronal degeneration and necrosis in the brains of CO-poisoned rats, and the escape latency of the CO Group in a Morris water maze became significantly longer than that of the Control Group (11.63 +/- 3.54s vs. 7.06 +/- 3.13s, p < 0.05) after six days of CO poisoning. Microglias, as immune effector cells, underwent activation and proliferation which reached 35.0 +/- 5.7 cells per five high-power fields (HPF) in the seventh day after CO poisoning, but 20.3 +/- 2.9 cells/5HPF in the Control Group (p < 0.05). Expression levels of immune factors increased in the brains of CO-poisoned rats. VCAM-1-positive cells peaked in quantity the first day, IFN-gamma-positive cells and MHCII-positive cells the third day and CD4-positive cells the seventh day. The results indicate that immune reaction plays an important role on CO-mediated neuropathology.
authors.affiliation: Department of Aerospace Hygiene and Health Service, School of Aerospace Medicine, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
authors.forename: Wenlan
authors.initials: W
authors.lastname: Wang
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Li
authors.forename: Yaoming
authors.initials: Y
authors.lastname: Chang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D001921:Brain / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015704:CD4 Antigens / Q000378:metabolism; D002249:Carbon Monoxide Poisoning / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D003072:Cognition Disorders / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism; D008285:Major Histocompatibility Complex / Q000276:immunology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D017628:Microglia / Q000473:pathology; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D009410:Nerve Degeneration / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D019010:Vascular Cell Adhesion Molecule-1; D007371:Interferon-gamma