title: In vitro and in vivo effects of Houttuynia cordata on infectious bronchitis virus.
abstract: Avian infectious bronchitis virus (IBV), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. Houttuynia cordata (Saururaceae) (HC) is a traditional Chinese medicine used in China. In the present study, the effect of HC on cell infection by IBV was determined using plaque assay and reverse transcription-polymerase chain reaction. The inhibitory effect of HC on IBV infection in ovo and in vivo was analysed using specific pathogen free (SPF) chicken embryos and chickens. Moreover, the effect of HC on cell apoptosis induced by IBV was investigated. Results showed that HC had more than 90% inhibition rate against IBV infection in Vero cells and chicken embryo kidney cells, and decreased more than 90% apoptotic cells caused by IBV. HC fully protected the SPF embryos, and had more than 50% protection rate in SPF chickens, against IBV challenge.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030, Harbin, China.
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D018352:Coronavirus Infections / Q000517:prevention & control / Q000662:veterinary*; D017931:DNA Primers / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D052859:Houttuynia; D001351:Infectious bronchitis virus / Q000187:drug effects*; D011201:Poultry Diseases / Q000517:prevention & control* / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013047:Specific Pathogen-Free Organisms; D014709:Vero Cells; D010948:Viral Plaque Assay
chemical_list: D017931:DNA Primers; D004365:Drugs, Chinese Herbal; C442781:Houttuynia cordata extract