title: Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging.
abstract: We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.
authors.affiliation: Sofia University, Department of Physical Chemistry, Lab. of Biophysical Chemistry, 1, James Bourchier Ave., Sofia 1164, Bulgaria. kbalashev@chem.uni-sofia.bg
authors.forename: Konstantin
authors.initials: K
authors.lastname: Balashev
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Callisen
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.forename: Thomas
authors.initials: T
authors.lastname: Bjørnholm
mesh_terms: D000388:Air; D000818:Animals; D002417:Cattle; D018625:Microscopy, Atomic Force / Q000379:methods*; D012697:Serine Endopeptidases / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D014867:Water
chemical_list: D014867:Water; D012710:Serum Albumin, Bovine; D012697:Serine Endopeptidases; C032923:microbial serine proteinases