title: Diagnosis of vaginal discharge by wet mount microscopy: a simple and underrated method.
abstract: Vaginal discharge is highly variable in quality and quantity among different individuals, and even in the same individual during different periods of life. Vaginal discharge is most commonly caused by infection with sexually transmitted organisms or increased colonization by different facultative pathogenic microorganisms (i.e., Gardnerella vaginalis). Noninfectious causes of vaginal discharge are quite rare (10% noninfectious as compared to 90% infectious causes). Most common in women with a vaginal infection is bacterial vaginosis (40%-50% of cases), followed by vulvovaginal candidosis (20%-25%), and then trichomoniasis (15%-20%). If infection is suspected as the primary cause, a sample of the vaginal discharge should be taken and examined microscopically. When evaluating vaginal secretions by phase-contrast wet mount microscopy, knowledge of what is normal versus abnormal is very important. Knowledge of the sensitivity and specificity of wet mount microscopy in different clinical settings is also important.
  
  
  BACKGROUND
  Obstetricians & Gynecologists, Family Physicians
  
  
  OBJECTIVE
  After completing this CME activity, physicians should be better able to evaluate lifetime changes in vaginal secretions, characterize the physiological and pathological appearance of vaginal discharge, assess the clinical practicality and usefulness of wet mount microscopy and use wet mount microscopy to diagnose bacterial vaginosis and other common vaginal infections.
authors.affiliation: Department of Obstetrics and Gynecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Munich, Germany. ioannis.mylonas@med.uni-muenchen.de
authors.forename: Ioannis
authors.initials: I
authors.lastname: Mylonas
authors.forename: Florian
authors.initials: F
authors.lastname: Bergauer
mesh_terms: D002181:Candidiasis, Vulvovaginal / Q000150:complications / Q000175:diagnosis; D005260:Female; D005833:Genital Neoplasms, Female / Q000150:complications / Q000175:diagnosis; D006801:Humans; D008853:Microscopy; D014247:Trichomonas Vaginitis / Q000150:complications / Q000175:diagnosis; D002575:Uterine Cervicitis / Q000150:complications / Q000175:diagnosis; D019522:Vaginal Discharge / Q000209:etiology* / Q000503:physiopathology; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000150:complications / Q000175:diagnosis