title: Neuronal differentiation potential of mouse induced pluripotent stem cells.
abstract: Induced pluripotent stem (iPS) cells have been generated from somatic cells by ectopic expression of defined transcription factors. The important issues for clinical applications of iPS cells are the defined methods for somatic cell differentiation and how to effectively enrich desired cell population. Here we used humanized renilla green fluorescent protein under the control of Tα1 α-tubulin promoter as lineage selection marker for neuronal differentiation of iPS cells. Using fluorescence-activated cell sorting, green fluorescent protein positive cells were isolated and enriched to near-purity. These results indicated that the neuronal differentiation potential of iPS cells derived from adult somatic cells is similar to that of embryonic stem cells and the high-purity neurons may have important implications for neurodevelopmental studies, safety pharmacological studies, and transplantation studies.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Yao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Ye
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Li
authors.forename: Xi-Lin
authors.initials: XL
authors.lastname: Lu
authors.forename: Pan-Long
authors.initials: PL
authors.lastname: Li
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Li
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Li
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000502:physiology