title: Using a TEMPO-based fluorescent probe for monitoring oxidative stress in living cells.
abstract: Generation of too many reactive oxygen species (ROS) in relation to available antioxidants in living cells can cause oxidative stress, which is involved in the development and progression of several serious diseases. 2',7'-Dichlorodihydrofluorescein (DCFH) and its diacetate form, DCFH-DA, are widely used probes for monitoring general oxidative stress in cells, but DCFH oxidation is not always related to ROS. We report here a new method for quantifying cellular oxidative stress using a 2,2,6,6-tetramethyl- piperidine-1-oxyl (TEMPO)-based probe. We tested and verified the probe both in cell-free solutions and in living cells under conditions of increased or reduced oxidative stress. The probe revealed the oxidative stress status in living cells and may be a useful complement to DCFH fluorescent probes.
authors.affiliation: Department of Nuclear Medicine, Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang 310009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Mingjiang
authors.initials: M
authors.lastname: Zhu
authors.forename: Jingji
authors.initials: J
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Tian
mesh_terms: D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer / Q000295:instrumentation*; D003497:Cyclic N-Oxides / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry / Q000633:toxicity; D005456:Fluorescent Dyes / Q000737:chemistry*; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D003497:Cyclic N-Oxides; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; C065013:2',7'-dichlorodihydrofluorescein; C003959:TEMPO