title: Energy metabolism in umbilical endothelial cells from preterm and term neonates.
abstract: The aim of this study was to investigate the impact of gestational age on energy metabolism in human umbilical vein endothelial cells (HUVECs) of preterm and term neonates.
  
  
  
  Activities of respiratory chain (RC) complexes I-V, citrate synthase (CS), overall mitochondrial fatty acid oxidation (FAO), carnitine palmitoyltransferase 2 (CPT2), glycolytic enzymes as well as energy-rich phosphates in HUVECs from uncomplicated term and preterm pregnancies were measured. Neonatal acylcarnitine profiles were analyzed postpartum.
  
  
  
  Activities of RC complexes II+III, IV, V, and CS were higher in HUVECs from immature pregnancies. Overall FAO did not change, whereas CPT2 activity was higher in term neonates. RC complexes II-V and CS correlated inversely to gestational age, as well as CPT2 activity within the term cohort. Phosphofructokinase activity increased with maturation; lactate dehydrogenase and hexokinase as well as energy-rich phosphates remained constant. In blood, long-chain acylcarnitines were higher in term neonates.
  
  
  
  Gestational age-dependent differences of energy-providing pathways in HUVECs were shown. Alterations of RC complexes with gestational age may be an adaptive process to cope with metabolic stress during birth; reduced oxidative phosphorylation and high glycolytic activity make HUVECs less susceptible to peripartum hypoxic damage. We hypothesize that HUVECs of premature neonates are metabolically maladapted to birth, which may be responsible for perinatal complications.
authors.affiliation: Clinic for Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Hannover, Germany. illsinger.sabine@mh-hannover.de
authors.forename: Sabine
authors.initials: S
authors.lastname: Illsinger
authors.forename: Nils
authors.initials: N
authors.lastname: Janzen
authors.forename: Stefanie
authors.initials: S
authors.lastname: Sander
authors.forename: Julia
authors.initials: J
authors.lastname: Bode
authors.forename: Lisa
authors.initials: L
authors.lastname: Mallunat
authors.forename: Rena
authors.initials: R
authors.lastname: Thomasmeyer
authors.forename: Friederike
authors.initials: F
authors.lastname: Hagebölling
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Schmidt
authors.forename: Jolanthe
authors.initials: J
authors.lastname: Bednarczyk
authors.forename: Bernhard
authors.initials: B
authors.lastname: Vaske
authors.forename: Thomas
authors.initials: T
authors.lastname: Lücke
authors.forename: Anibh M
authors.initials: AM
authors.lastname: Das
mesh_terms: D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D015331:Cohort Studies; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism*; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006019:Glycolysis; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D008297:Male; D010085:Oxidative Phosphorylation; D011247:Pregnancy; D011446:Prospective Studies; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
chemical_list: D045222:Electron Transport Chain Complex Proteins; D005227:Fatty Acids; D002334:Carnitine O-Palmitoyltransferase; D002950:Citrate (si)-Synthase