title: Growth factor- and adhesion protein-like components of fetal calf serum can significantly enhance the intracellular delivery of Peptide nucleic acids.
abstract: Evidence is presented that components of fetal calf serum (FCS) can significantly enhance the splicing correction activity of peptide nucleic acids (PNA) in HeLa pLuc 705 cells. The effect proved more pronounced for PNAs bearing fluorescence tags and relies on the ability of specific components of FCS to mediate a mainly nonendocytotic intracellular delivery of PNA. Attempts to isolate and characterize the active serum components using PNA-loaded beads and nano-LC-ESI mass spectrometry revealed the growth-factor related inter-alpha-trypsin inhibitor and the adhesion protein fibronectin to be substantially responsible for the delivery activity of FCS.
authors.affiliation: Leibniz-Institute of Molecular Pharmacology , Berlin, Germany. joehlke@t-online.de
authors.forename: Johannes
authors.initials: J
authors.lastname: Oehlke
authors.forename: Angelika
authors.initials: A
authors.lastname: Ehrlich
authors.forename: Eberhard
authors.initials: E
authors.lastname: Krause
authors.forename: Stephan
authors.initials: S
authors.lastname: Pritz
authors.forename: Burkhard
authors.initials: B
authors.lastname: Wiesner
authors.forename: Michael
authors.initials: M
authors.lastname: Beyermann
mesh_terms: D000510:Alpha-Globulins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002846:Chromatography, Affinity; D004337:Drug Carriers / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005312:Fetal Blood / Q000737:chemistry / Q000378:metabolism*; D005353:Fibronectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005434:Flow Cytometry; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D012326:RNA Splicing / Q000187:drug effects; D044967:Serum / Q000737:chemistry / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000510:Alpha-Globulins; D004337:Drug Carriers; D005353:Fibronectins; D020135:Peptide Nucleic Acids; C011877:inter-alpha-inhibitor; D008156:Luciferases