title: The use of nitroxide radical-containing nanoparticles coupled with piperine to protect neuroblastoma SH-SY5Y cells from Aβ-induced oxidative stress.
abstract: The antioxidant effect and potential mechanism of nitroxide radical-containing nanoparticles (RNPs) coupled with piperine (PI) were investigated in human neuroblastoma SH-SY5Y cells. The effects of RNP/PI on SH-SY5Y cell lines was determined by WST assay for cell viability, nitroblue tetrazolium and deoxyribose assay for reactive oxygen species generation, ELISA assay for reactive oxygen species products and apoptotic cell death, and biochemical techniques for catalase and glutathione peroxidase activity. The RNP/PI significantly reduced the reactive oxygen species level and reactive oxygen species products compared with those of cells treated with RNPs alone. The RNP/PI treatment enhanced catalase and glutathione peroxidase activity. The combination of RNP/PI has been found to have an augmented antioxidant effect on an Alzheimer's model in vitro. The mechanism of the protective effect of this combination therapy was correlated in this study with its ability to reduce the generation of reactive oxygen species and prevent apoptosis via scavenging enzyme action pathways.
authors.affiliation: Department of Materials Science, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8503, Japan.
authors.forename: Pennapa
authors.initials: P
authors.lastname: Chonpathompikunlert
authors.forename: Toru
authors.initials: T
authors.lastname: Yoshitomi
authors.forename: Junkyu
authors.initials: J
authors.lastname: Han
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nagasaki
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D016229:Amyloid beta-Peptides / Q000502:physiology*; D017209:Apoptosis; D052117:Benzodioxoles / Q000494:pharmacology*; D045744:Cell Line, Tumor; D006801:Humans; D053758:Nanoparticles; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009589:Nitrogen Oxides / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000470:Alkaloids; D016229:Amyloid beta-Peptides; D052117:Benzodioxoles; D009589:Nitrogen Oxides; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D017382:Reactive Oxygen Species; C008922:piperine