title: Flowering time variation in oilseed rape (Brassica napus L.) is associated with allelic variation in the FRIGIDA homologue BnaA.FRI.a.
abstract: Oilseed rape (Brassica napus L.) is a major oil crop which is grown worldwide. Adaptation to different environments and regional climatic conditions involves variation in the regulation of flowering time. Winter types have a strong vernalization requirement whereas semi-winter and spring types have a low vernalization requirement or flower without exposure to cold, respectively. In Arabidopsis thaliana, FRIGIDA (FRI) is a key regulator which inhibits floral transition through activation of FLOWERING LOCUS C (FLC), a central repressor of flowering which controls vernalization requirement and response. Here, four FRI homologues in B. napus were identified by BAC library screening and PCR-based cloning. While all homologues are expressed, two genes were found to be differentially expressed in aerial plant organs. One of these, BnaA.FRI.a, was mapped to a region on chromosome A03 which co-localizes with a major flowering time quantitative trait locus in multiple environments in a doubled-haploid mapping population. Association analysis of BnaA.FRI.a revealed that six SNPs, including at least one at a putative functional site, and one haplotype block, respectively, are associated with flowering time variation in 248 accessions, with flowering times differing by 13-19 d between extreme haplotypes. The results from both linkage analysis and association mapping indicate that BnaA.FRI.a is a major determinant of flowering time in oilseed rape, and suggest further that this gene also contributes to the differentiation between growth types. The putative functional polymorphisms identified here may facilitate adaptation of this crop to specific environments through marker-assisted breeding.
authors.affiliation: Plant Breeding Institute, Christian-Albrechts-University of Kiel, Olshausenstr. 40, D-24098 Kiel, Germany.
authors.forename: Nian
authors.initials: N
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.forename: Ida
authors.initials: I
authors.lastname: Suppanz
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wei
authors.forename: Bizeng
authors.initials: B
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.forename: Jinling
authors.initials: J
authors.lastname: Meng
authors.forename: Andreas E
authors.initials: AE
authors.lastname: Müller
authors.forename: Christian
authors.initials: C
authors.lastname: Jung
mesh_terms: D000483:Alleles; D029688:Brassica napus / Q000235:genetics* / Q000502:physiology*; D035264:Flowers / Q000235:genetics* / Q000502:physiology*; D014644:Genetic Variation / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics
chemical_list: D010940:Plant Proteins