title: Functional HAK/KUP/KT-like potassium transporter encoded by chlorella viruses.
abstract: Chlorella viruses are a source of interesting membrane transport proteins. Here we examine a putative K(+)  transporter encoded by virus FR483 and related chlorella viruses. The protein shares sequence and structural features with HAK/KUP/KT-like K(+)  transporters from plants, bacteria and fungi. Yeast complementation assays and Rb(+)  uptake experiments show that the viral protein, termed HAKCV (high-affinity K(+)  transporter of chlorella virus), is functional, with transport characteristics that are similar to those of known K(+)  transporters. Expression studies revealed that the protein is expressed as an early gene during viral replication, and proteomics data indicate that it is not packaged in the virion. The function of HAKCV is unclear, but the data refute the hypothesis that the transporter acts as a substitute for viral-encoded K(+)  channels during virus infection.
authors.affiliation: Institute of Botany at the Technische Universität Darmstadt, Schnittspahnstrasse 3-5, 64287 Darmstadt, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Greiner
authors.forename: José
authors.initials: J
authors.lastname: Ramos
authors.forename: Maria C
authors.initials: MC
authors.lastname: Alvarez
authors.forename: James R
authors.initials: JR
authors.lastname: Gurnon
authors.forename: Ming
authors.initials: M
authors.lastname: Kang
authors.forename: James L
authors.initials: JL
authors.lastname: Van Etten
authors.forename: Anna
authors.initials: A
authors.lastname: Moroni
authors.forename: Gerhard
authors.initials: G
authors.lastname: Thiel
mesh_terms: D000595:Amino Acid Sequence; D027682:Cation Transport Proteins; D002708:Chlorella / Q000821:virology*; D008969:Molecular Sequence Data; D017862:Phycodnaviridae / Q000502:physiology*; D010940:Plant Proteins; D011188:Potassium; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D027682:Cation Transport Proteins; D010940:Plant Proteins; D014764:Viral Proteins; D011188:Potassium