title: Evaluation of VEGF-induced vascular permeability in mice.
abstract: Vascular endothelial growth factor (VEGF) is a potent inducer of angiogenesis and vascular leak involved in development, wound healing, tumor growth, macular degeneration, and ischemia. Studying the effects of VEGF in vitro is not always sufficient to approximate the complex in vivo response that involves multiple cell types within functioning tissues. Treating mice with an intravenous injection of recombinant VEGF produces a rapid and transient biochemical response that is accompanied by a series of ultrastructural changes. Similar events are induced by hypoxia-induced VEGF in the heart following myocardial infarction or by tumor cell-released VEGF during metastasis. Studying how intact blood vessels respond to VEGF will augment the further development of antipermeability strategies to improve disease progression in a number of pathologies.
authors.affiliation: Moores UCSD Cancer Center, University of California, San Diego, La Jolla, CA, USA. sweis@ucsd.edu
authors.forename: Sara M
authors.initials: SM
authors.lastname: Weis
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis; D001681:Biological Assay; D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis / Q000096:biosynthesis; D002199:Capillary Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D004338:Drug Combinations; D004591:Electrophoresis, Polyacrylamide Gel; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D000860:Hypoxia / Q000378:metabolism; D007275:Injections, Intravenous; D007797:Laminin / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D046529:Microscopy, Electron, Transmission; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013045:Species Specificity; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000494:pharmacology; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D003094:Collagen; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D042461:Vascular Endothelial Growth Factor A; D040301:Vascular Endothelial Growth Factor Receptor-2; D000099152:Cadherin 5; C059485:matrigel