title: Diagnostic usefulness of a lumi-aggregometer adenosine triphosphate release assay for the assessment of platelet function disorders.
abstract: Platelet dense granule release assays are recommended for diagnosing platelet function disorders and are commonly performed by Lumi-Aggregometer (Chrono-Log, Havertown, PA) assays of adenosine triphosphate (ATP) release. We conducted a prospective cohort study of people tested for ATP release defects to assess bleeding symptoms. Reduced release, with 1 or more agonists, was more common among patients with bleeding disorders than among healthy control subjects (P < .001). The respective likelihood (odds ratio [95% confidence interval]) of a bleeding disorder or an inherited platelet function disorder were high when release was reduced with 1 or more agonists (17 [6-46]; 128 [30-545]), even if aggregation was normal (12 [4-34]; 105 [20-565]). ATP release had high specificity and moderate sensitivity for inherited platelet function disorders, with most abnormalities detected by the combination of 6 μmol/L epinephrine, 5.0 μg/mL collagen, and 1 μmol/L U46619. Platelet ATP release assays are useful for evaluating common bleeding disorders, regardless of aggregation findings.
authors.affiliation: Department of Medicine, McMaster University, Hamilton, Canada.
authors.forename: Menaka
authors.initials: M
authors.lastname: Pai
authors.forename: Grace
authors.initials: G
authors.lastname: Wang
authors.forename: Karen A
authors.initials: KA
authors.lastname: Moffat
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jodi
authors.initials: J
authors.lastname: Seecharan
authors.forename: Kathryn
authors.initials: K
authors.lastname: Webert
authors.forename: Nancy
authors.initials: N
authors.lastname: Heddle
authors.forename: Catherine
authors.initials: C
authors.lastname: Hayward
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001791:Blood Platelet Disorders / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation; D010979:Platelet Function Tests / Q000295:instrumentation*; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D000255:Adenosine Triphosphate