title: [Participation of beta2-integrins CD11b/CD18 and CD11c/CD18 in adhesion and migration of cells on fibrinogen].
abstract: The role of beta2-integrins CD11b/CD18 and CD 11c/CD 18 in adhesion and migration of leukocytes on fibrinogen was studied. The monoclonal antibodies against CD11b inhibited the spontaneous adhesion of monocytic THP-1 cells on fibrinogen, whereas antibodies to CD11c more effectively inhibited the adhesion stimulated by chemokine MCP-1. By the RNA-interference method the clones of THP-1 with reduced expression of CD11b and general beta2-subunit CD18 were obtained. MCP-I stimulated the adhesion to fibrinogen of THP-1 cells of wild-type and mutant cells with reduced expression of CD11b (THP-1-CD11b-low), but not of cells with low expression of CD18 (THP-1-CD18-low). THP-1-CD18-low cells were also characterized by the impaired chemotaxis in presence of MCP-1. The data obtained suggest that spontaneous cell adhesion to fibrinogen is mediated to a greater extent by CD11b/CD18 integrins, while chemokine-stimulated adhesion and migration is mostly dependent on CD11c/CD18 molecules.
authors.forename: N B
authors.initials: NB
authors.lastname: Kukhtina
authors.forename: P N
authors.initials: PN
authors.lastname: Rutkevich
authors.forename: A Ia
authors.initials: AIa
authors.lastname: Shevelev
authors.forename: T N
authors.initials: TN
authors.lastname: Vlasik
authors.forename: T I
authors.initials: TI
authors.lastname: Aref'eva
mesh_terms: D039481:CD11b Antigen / Q000235:genetics / Q000502:physiology*; D039521:CD11c Antigen / Q000235:genetics / Q000502:physiology*; D018821:CD18 Antigens / Q000235:genetics / Q000502:physiology; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000502:physiology; D009000:Monocytes / Q000502:physiology
chemical_list: D039481:CD11b Antigen; D039521:CD11c Antigen; D018821:CD18 Antigens; D005340:Fibrinogen