title: Biofilm production using distinct media and antimicrobial susceptibility profile of Pseudomonas aeruginosa.
abstract: Biofilm production is an important mechanism for bacterial survival and its occurrence together with antimicrobial resistance represents a challenge for clinical management. Here, we evaluated the ability for biofilm production among P. aeruginosa isolates from patients with or without cystic fibrosis (CF) using two distinct media, besides determining the antimicrobial susceptibility profile of these isolates for eight antimicrobial agents. The ability for biofilm production when TSB medium was used was higher than when used CF sputum media (p = 0.0198). However, P. aeruginosa isolates from CF have demonstrated similar performance for biofilm production, independently of the medium used. Besides, among the biofilm-producing isolates, those recovered from CF were more resistant to the carbapenems (meropenem and imipenem) agents than those isolates from non-CF isolates.
authors.affiliation: Microbiology Unit, Hospital Mãe de Deus, Porto Alegre, RS, Brazil. leandro.reus@gmail.com
authors.forename: Leandro Reus Rodrigues
authors.initials: LR
authors.lastname: Perez
authors.forename: Afonso Luís
authors.initials: AL
authors.lastname: Barth
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D003470:Culture Media; D003550:Cystic Fibrosis / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media