title: N-phenylmaleimide derivatives as mimetic agents of the pro-inflammatory process: myeloperoxidase activation.
abstract: Myeloperoxidase (MPO) is an important enzyme that catalyzes the reaction between hydrogen peroxide and chloride to generate hypochlorous acid, which oxidizes a range of biomolecules and has been associated with inflammatory diseases. The synthetic compounds N-phenylmaleimide (NFM) and 4-methyl-N-phenylmaleimide (Me-NFM) increased the MPO activity in vitro (of isolated enzyme and in isolated cells after animal treatment) and in vivo assays. MPO-induction may represent a good model system to investigate the molecular and cellular mechanisms of oxidative cell injury induced by activated neutrophils, and the interactions between damaging species involved in the respiratory burst.
authors.affiliation: Department of Pharmaceutical Sciences, Center of Science and Health, Federal University of Santa Catarina, 88040-900 Florianópolis-SC, Brazil.
authors.forename: Vânia F
authors.initials: VF
authors.lastname: Noldin
authors.forename: Silvana V G
authors.initials: SV
authors.lastname: Vigil
authors.forename: Rafael
authors.initials: R
authors.lastname: De Liz
authors.forename: Valdir
authors.initials: V
authors.lastname: Cechinel-Filho
authors.forename: Tânia S
authors.initials: TS
authors.lastname: Fröde
authors.forename: Tânia B
authors.initials: TB
authors.lastname: Creczynski-Pasa
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D008301:Maleimides / Q000494:pharmacology*; D051379:Mice; D009504:Neutrophils / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: C568561:4-methyl-N-phenylmaleimide; D008301:Maleimides; C028525:N-phenylmaleimide; D009195:Peroxidase