title: Fabrication of polyphenol biosensor based on laccase immobilized on copper nanoparticles/chitosan/multiwalled carbon nanotubes/polyaniline-modified gold electrode.
abstract: A high-performance amperometric polyphenol biosensor was developed, based on covalent immobilization of Ganoderma sp. laccase onto copper nanoparticles (CuNP's)/chitosan (CHIT)/carboxylated multiwalled carbon nanotube (cMWCNT)/polyaniline (PANI)-modified gold (Au) electrode. The CuNP's and cMWCNT had a synergistic electrocatalytic effect in the matrix of CHIT. The biosensor showed optimum response at pH 6.0 (0.1 M acetate buffer) and 35°C, when operated at 50 mVs(-1). The biosensor exhibited excellent sensitivity (the detection limit was down to 0.156 μM for guaiacol), fast response time (less than 4s) and wide linear range (from 1 to 500 μM). Analytical recovery of added guaiacol was 96.40-98.46%. Within batch and between batch coefficients of variation were <2.6% and <5.3%, respectively. The enzyme electrode was used 300 times over a period of 7 months, when stored at 4°C.
authors.affiliation: Department of Biochemistry, M.D. University, Rohtak 124 001, Haryana, India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Chawla
authors.forename: Rachna
authors.initials: R
authors.lastname: Rawal
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D048271:Chitosan / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D017743:Equipment Reuse; D032902:Ganoderma / Q000201:enzymology; D006046:Gold / Q000737:chemistry; D006139:Guaiacol / Q000032:analysis; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D016014:Linear Models; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D059808:Polyphenols; C416807:polyaniline; D006139:Guaiacol; D006046:Gold; D003300:Copper; D048271:Chitosan; D042845:Laccase