title: MRI of CEST-based reporter gene.
abstract: In recent years, several reporter genes have been designed for non-invasive magnetic resonance imaging (MRI). Here, we offer a brief summary of recent advances in MRI reporter gene technology, as well as elaborated protocols for cloning, expression, and imaging of reporter genes based on a chemical exchange saturation transfer (CEST) method. These protocols emphasize new developments in CEST-MRI data acquisition and processing.
authors.affiliation: Department of Radiology, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, USA. guanshu@mri.jhu.edu
authors.forename: Guanshu
authors.initials: G
authors.lastname: Liu
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
mesh_terms: D017930:Genes, Reporter; D008279:Magnetic Resonance Imaging / Q000379:methods*