title: Coenzyme Q10 protects neurons against neurotoxicity in hippocampal slice culture.
abstract: This study investigated the neuroprotective effects of coenzyme Q10 (CoQ10) against oxidative stress induced by kainic acid (KA) in organotypic hippocampal slice culture of rats. Cultured slices were injured by exposure to 5 µM of KA for 18 h and then treated with different concentrations of CoQ10. Neuronal cell death measured as propidium iodide uptake was reduced at 24 h after treatment with 1 µM of CoQ10. We also observed an increased number of surviving CA3 neurons in 0.1 and 1 µM concentrations of CoQ10-treated groups using cresyl violet staining. CoQ10 (0.01, 0.1, and 1 µM) treatment significantly decreased the 2',7'-dichlorofluorescein fluorescence and the expression of NQO1 in the CoQ10-treated groups was significantly lower than that in the KA-only group. These results suggest that CoQ10 may protect hippocampal neurons against oxidative stress.
authors.affiliation: Department of Biomedical Laboratory Science, Dongseo University, Busan, Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Won
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Lee
authors.forename: Bae Hwan
authors.initials: BH
authors.lastname: Lee
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D007608:Kainic Acid / Q000633:toxicity; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000502:physiology
chemical_list: D018696:Neuroprotective Agents; D014451:Ubiquinone; C024989:coenzyme Q10; D007608:Kainic Acid