title: Synergistic effect of biochemical factors and strain on the smooth muscle cell differentiation of adipose-derived stem cells on an elastic nanofibrous scaffold.
abstract: Adipose-derived stem cells (ASCs) have been the subject of many tissue-engineering studies, mainly because of their multipotential properties. We have focused on the potential of human ASCs (hASCs) for smooth muscle cell (SMC) differentiation in cardiovascular tissue engineering. In this study, we investigated the combined effect of differentiation factors along with strain utilizing a customized device on hASCs proliferation and consequent differentiation into SMCs on an elastic nanofibrous scaffold. The cell proliferation increased in strain-stimulated culture and was more affected by media composition as compared to in static culture. Differentiation factors did not have an influence on SM α-actin (α-SMA) and myosin heavy chain (MHC) expression in static culture. However, α-SMA and MHC expression were affected by differentiation factors in strain culture, in particular, showing that treatment with retinoic acid significantly increased the expression of α-SMA (3.6-fold) and MHC (2-fold) as compared to strain alone. This study demonstrated the synergic effect of strain and biochemical factor on the SMC differentiation of hASCs and provided a useful method for the application of stem cells in mechano-active tissue engineering.
authors.affiliation: a Center for Biomaterials, Medical Engineering Institute, Korea Institute of Science and Technology , 39-1 Hawolgok , Seongbuk , Seoul , 136-791 , South Korea.
authors.forename: In Su
authors.initials: IS
authors.lastname: Park
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.forename: Youngmee
authors.initials: Y
authors.lastname: Jung
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Rhie
authors.forename: Sang-Heon
authors.initials: SH
authors.lastname: Kim
mesh_terms: D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000502:physiology*; D000328:Adult; D018929:Cell Culture Techniques / Q000295:instrumentation; D002454:Cell Differentiation / Q000502:physiology*; D004548:Elasticity; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D018995:Myosin Heavy Chains / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D013234:Stem Cells / Q000502:physiology*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000199:Actins; D018995:Myosin Heavy Chains