title: The alterations in hepatic microcirculation and Kupffer cell activity after biliary drainage in jaundiced mice.
abstract: OBJECTIVE
  The aim of this study is to examine the effects of biliary drainage on hepatic microcirculation and Kupffer cell activity in the liver with obstructive jaundice.
  
  
  METHODS
  Common bile duct ligation and division was performed on C57BL/6 mice to induce obstructive jaundice. Seven or 14 days after surgery, some mice underwent biliary drainage. Three days after biliary drainage, sinusoidal perfusion, leukocyte rolling and sticking in the postsinusoidal venules, and the diameters of sinusoids containing blood flow were evaluated using intravital microscopy. Kupffer cell phagocytic activity was estimated as the ratio of Kupffer cells that phagocytosed fluorescent-labeled particles to sinusoids containing blood flow.
  
  
  RESULTS
  Sinusoidal perfusion after biliary drainage was significantly increased compared with that in livers with obstructive jaundice, but remained decreased compared with controls. Although the number of rolling leukocytes and sticking leukocytes was significantly decreased, the diameters of sinusoids remained reduced, associated with an increase in Kupffer cell phagocytic activity compared with controls even after biliary drainage.
  
  
  CONCLUSIONS
  Leukocyte-endothelial cell interaction is ameliorated but sinusoids remain narrowed due to swelling of activated Kupffer cells; this might cause deterioration of hepatic microcirculation during the early phase of biliary drainage.
authors.affiliation: Department of Surgery, Chibaken Saiseikai Narashino Hospital, 1-1-1 Izumichou, Narashino, Chiba 275-8580, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Okaya
authors.forename: Koji
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Fumio
authors.initials: F
authors.lastname: Kimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimizu
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshidome
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kato
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Yoshitomi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ito
authors.forename: Masaru
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D004322:Drainage; D041781:Jaundice, Obstructive; D007728:Kupffer Cells / Q000502:physiology*; D008099:Liver / Q000098:blood supply* / Q000166:cytology / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology
chemical_list: D000410:Alanine Transaminase; D001663:Bilirubin