title: Cellular viability effects of fatty acid amide hydrolase inhibition on cerebellar neurons.
abstract: The endocannabinoid anandamide (ANA) participates in the control of cell death inducing the formation of apoptotic bodies and DNA fragmentation. The aim of this study was to evaluate whether the ANA degrading enzyme, the fatty acid amide hydrolase (FAAH), would induce cellular death. Experiments were performed in cerebellar granule neurons cultured with the FAAH inhibitor, URB597 (25, 50 or 100 nM) as well as endogenous lipids such as oleoylethanolamide (OEA) or palmitoylethanolamide (PEA) and cellular viability was determined by MTT test. Neurons cultured with URB597 (25, 50 or 100 nM) displayed a decrease in cellular viability. In addition, if cultured with OEA (25 nM) or PEA (100 nM), cellular death was found. These results further suggest that URB597, OEA or PEA promote cellular death.
authors.affiliation: Department of Neurology, Philipps University, D-35033 Marburg, Germany. arias@exp-neuro.de.
authors.forename: Kathia
authors.initials: K
authors.lastname: Lueneberg
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Domínguez
authors.forename: Oscar
authors.initials: O
authors.lastname: Arias-Carrión
authors.forename: Marcela
authors.initials: M
authors.lastname: Palomero-Rivero
authors.forename: Diana
authors.initials: D
authors.lastname: Millán-Aldaco
authors.forename: Julio
authors.initials: J
authors.lastname: Morán
authors.forename: René
authors.initials: R
authors.lastname: Drucker-Colín
authors.forename: Eric
authors.initials: E
authors.lastname: Murillo-Rodríguez