title: Genetic diversity of Borrelia burgdorferi sensu lato isolates from Northeastern China.
abstract: Thirty-two strains of Borrelia burgdorferi sensu lato were isolated from Ixodes persulcatus ticks collected from northeastern China from May to June in 2004 and 2005. Restriction fragment length polymorphism (RFLP) analysis and sequence analysis of 5S-23S rRNA intergenic spacer revealed that 29 (90.6%) belonged to Borrelia garinii, demonstrating B, C, and a unique pattern. The remaining three isolates (9.4%) were Borrelia afzelii with pattern D. The phylogenetic analysis based on 5S-23S rRNA intergenic spacer showed that B. garinii and B. afzelii genospecies clustered into two separate lineages. B. garinii strains were classified into three different branches: All the strains with RFLP pattern C were in the same branch, strain VH10 with a unique RFLP pattern clustered with strains VH9 and MDH2 with pattern B, and the rest of the strains with pattern B constitute another branch. These findings demonstrate the genetic diversity of B. burgdorferi sensu lato isolates from northeastern China.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China.
authors.forename: Chen-Yi
authors.initials: CY
authors.lastname: Chu
authors.forename: Bao-Gui
authors.initials: BG
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Pan-He
authors.initials: PH
authors.lastname: Zhang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wu
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Hua
authors.initials: H
authors.lastname: Shi
authors.forename: Ha-Sen
authors.initials: HS
authors.lastname: Gaowa
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Janet E
authors.initials: JE
authors.lastname: Foley
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Cao
mesh_terms: D000818:Animals; D015748:Borrelia burgdorferi Group / Q000145:classification / Q000235:genetics*; D002681:China; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D003870:Dermacentor; D014644:Genetic Variation; D018884:Ixodes / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S; D017421:Sequence Analysis; D013987:Ticks / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D012338:RNA, Ribosomal, 23S