title: Characterization of Baylisascaris schroederi from Qinling subspecies of giant panda in China by the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA.
abstract: In the present study, a total of 20 nematode isolates, (including 10 male and 10 female worms) representing Baylisascaris schroederi from 5 Qinling subspecies of giant pandas (Ailuropoda melanoleuca) in Shaanxi Province of China, were characterized and grouped genetically by the first internal transcribed spacer (ITS-1) of nuclear ribosomal DNA (rDNA). The rDNA fragment spanning 3' end of 18S rDNA, complete ITS-1 rDNA, and 5' end of 5.8S rDNA were amplified and sequenced. The sequence variability in ITS-1 rDNA was examined within B. schroederi and among parasites in order Ascaridata available in GenBank™, and their phylogenetic relationships were also reconstructed. The sequences of ITS-1 rDNA for all the B. schroederi isolates were 427 bp in length, with no genetic variation detected among these isolates. Phylogenetic analyses based on the ITS-1 rDNA sequences revealed that all the male and female B. schroederi isolates sequenced in the present study were posited into the clade of genus Baylisascaris, sistered to zoonotic nematodes in genus Ascaris, and the ITS-1 rDNA sequence could distinguish different species in order Ascaridata. These results showed that the ITS-1 rDNA provides a suitable molecular marker for the inter-species phylogenetic analysis and differential identification of nematodes in order Ascaridata.
authors.affiliation: College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
authors.forename: Q
authors.initials: Q
authors.lastname: Lin
authors.forename: H M
authors.initials: HM
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Gao
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: W X
authors.initials: WX
authors.lastname: Ren
authors.forename: M M
authors.initials: MM
authors.lastname: Cong
authors.forename: X C
authors.initials: XC
authors.lastname: Tan
authors.forename: C X
authors.initials: CX
authors.lastname: Chen
authors.forename: S K
authors.initials: SK
authors.lastname: Yu
authors.forename: G H
authors.initials: GH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017191:Ascaridida Infections / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002681:China; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis* / Q000235:genetics; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D008297:Male; D010274:Parasitology / Q000379:methods; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D001503:Ursidae / Q000469:parasitology*
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers