title: Poor diagnostic accuracy of commercial antibody-based assays for the diagnosis of acute Chikungunya infection.
abstract: A Sri Lankan fever cohort (n = 292 patients; 17.8% prevalence) was used to assess two standard diagnostic Chikungunya IgM tests. The immunochromatographic test (ICT) acute sample sensitivity (SN) was 1.9 to 3.9%, and specificity (SP) was 92.5 to 95.0%. The enzyme-linked immunosorbent assay (ELISA) gave an acute sample SN of 3.9% and an SP of 92.5% and a convalescent sample SN of 84% and an SP of 91%. These assays are not suitable for the acute diagnosis of Chikungunya virus infection.
authors.affiliation: Mahidol University—Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. stuart@tropmedres.ac
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Blacksell
authors.forename: Ampai
authors.initials: A
authors.lastname: Tanganuchitcharnchai
authors.forename: Richard G
authors.initials: RG
authors.lastname: Jarman
authors.forename: Robert V
authors.initials: RV
authors.lastname: Gibbons
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Paris
authors.forename: Mark S
authors.initials: MS
authors.lastname: Bailey
authors.forename: Nicholas P J
authors.initials: NP
authors.lastname: Day
authors.forename: Ranjan
authors.initials: R
authors.lastname: Premaratna
authors.forename: David G
authors.initials: DG
authors.lastname: Lalloo
authors.forename: H Janaka
authors.initials: HJ
authors.lastname: de Silva
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis*; D000914:Antibodies, Viral / Q000097:blood*; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000276:immunology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D015331:Cohort Studies; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity; D013188:Sri Lanka
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M