title: Serum paraoxonase-3 concentration is associated with the severity of hepatic impairment in patients with chronic liver disease.
abstract: OBJECTIVE
  Research on paraoxonase-3 (PON3) has been hampered by the lack of methods for measurement. This is a pilot study aimed at exploring whether chronic liver impairment is associated with changes in serum PON3 concentrations, and to know whether this measurement may provide useful information to investigate this derangement.
  
  
  METHODS
  We studied 110 patients with chronic liver disease (21 minimal changes, 79 chronic hepatitis, 10 cirrhosis) and 356 healthy volunteers. Serum PON3 concentration was determined by ELISA using polyclonal antibodies generated against a synthetic peptide with a sequence specific to PON3.
  
  
  RESULTS
  Serum PON3 concentrations were increased in patients with chronic hepatitis or cirrhosis and showed significant direct correlations with the degree of periportal abnormalities including fibrosis, and with serum FAS (a marker of antiapoptosis) concentrations.
  
  
  CONCLUSIONS
  These results suggest that PON3 may play a hepatoprotective role against histological alterations and hepatic cell apoptosis leading to liver disease.
authors.affiliation: Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, 43201 Reus, Catalonia, Spain.
authors.forename: Anabel
authors.initials: A
authors.lastname: García-Heredia
authors.forename: Judit
authors.initials: J
authors.lastname: Marsillach
authors.forename: Gerard
authors.initials: G
authors.lastname: Aragonès
authors.forename: Marta
authors.initials: M
authors.lastname: Guardiola
authors.forename: Anna
authors.initials: A
authors.lastname: Rull
authors.forename: Raúl
authors.initials: R
authors.lastname: Beltrán-Debón
authors.forename: Alba
authors.initials: A
authors.lastname: Folch
authors.forename: Bharti
authors.initials: B
authors.lastname: Mackness
authors.forename: Michael
authors.initials: M
authors.lastname: Mackness
authors.forename: Juan
authors.initials: J
authors.lastname: Pedro-Botet
authors.forename: Jorge
authors.initials: J
authors.lastname: Joven
authors.forename: Jordi
authors.initials: J
authors.lastname: Camps
mesh_terms: D000328:Adult; D000368:Aged; D043303:Aryldialkylphosphatase / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood / Q000175:diagnosis / Q000201:enzymology*; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood / Q000175:diagnosis / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects
chemical_list: D015415:Biomarkers; D043303:Aryldialkylphosphatase; C113656:PON3 protein, human