title: [The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure].
abstract: OBJECTIVE
  To investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF).
  
  
  METHODS
  Patients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of IL-10 promoter via MSP, to analyze the difference among the three groups.
  
  
  RESULTS
  Both the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group (P < 0.05); the ACLF group's level is higher than the CHB group, however without statistical significance (P > 0.05). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0.022, r = 0.033, respectively; P > 0.05); has positive relativity with TBIL and MELD ( r = 0.566, r = 0.443, respectively; P < 0.05); and negative relativity with PTA (r = -0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two.
  
  
  CONCLUSIONS
  The serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.
authors.affiliation: QiLu Hospital of ShangDong University, Shandong 250012, China.
authors.forename: Zhao-xia
authors.initials: ZX
authors.lastname: Qi
authors.forename: Shu-xia
authors.initials: SX
authors.lastname: Yu
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Hao
authors.forename: Feng-cai
authors.initials: FC
authors.lastname: Li
authors.forename: Zhao-yang
authors.initials: ZY
authors.lastname: Guo
authors.forename: Yu-chen
authors.initials: YC
authors.lastname: Fan
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D002908:Chronic Disease; D019175:DNA Methylation; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000235:genetics* / Q000378:metabolism; D017114:Liver Failure, Acute / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008745:Methylation; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D055815:Young Adult
chemical_list: D016753:Interleukin-10