title: Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional alleles.
abstract: Transgenic and conditional knockout mouse models play an important role in biomedical research and their use has grown exponentially in the last 5-10 years. Generating conditional knockouts often requires breeding multiple alleles onto the background of a single mouse or group of mice. Breeding these mice depends on parental genotype, litter size, transmission frequency, and the number of breeding rounds. Therefore, a well planned breeding strategy is critical for keeping costs to a minimum. However, designing a viable breeding strategy can be challenging. With so many different variables this would be an ideal task for a computer program. To facilitate this process, we created a Java-based program called Conditional Allele Mouse Planner (CAMP). CAMP is designed to provide an estimate of the number of breeders, amount of time, and costs associated with generating mice of a particular genotype. We provide a description of CAMP, how to use it, and offer it freely as an application.
authors.affiliation: Epithelial Systems Biology Group, Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 10, 6N312, Bethesda, MD 20892, USA.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Hoffert
authors.forename: Trairak
authors.initials: T
authors.lastname: Pisitkun
authors.forename: R Lance
authors.initials: RL
authors.lastname: Miller
mesh_terms: D000465:Algorithms; D000483:Alleles; D000818:Animals; D001947:Breeding / Q000379:methods*; D005260:Female; D005838:Genotype; D040582:Inheritance Patterns; D008098:Litter Size; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D012984:Software; D013997:Time Factors