title: Interaction of glycated human serum albumin with endothelial cells in a hemodynamic environment: structural and functional correlates.
abstract: Advanced glycation end products (AGEs) are known to be involved in the pathogenesis of several diseases, in particular diabetes, via signaling through their receptor. Numerous studies have been carried out on protein-sugar interactions at very high concentrations of the latter. The objective of this investigation was to determine the effects of nonenzymatic glycation induced by reducing sugars on the secondary structure of human serum albumin (HSA) under different physiological conditions and to correlate that with expression of RAGE (receptor for advanced glycation end products) on HUVECs (human umbilical vein endothelial cells) in a controlled hemodynamic environment. Our results indicate that RAGE expression is shear stress modulated and that glycated HSA enhances the expression further. The secondary structure of AGE-HSA derived from glucose at 20 mM contains higher α-helical content and elicits maximum expression of the receptor. The effect of shear stress at 10 dynes cm(-2) is independent of AGE-HSA.
authors.affiliation: Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi 110 016, India.
authors.forename: Kanchan
authors.initials: K
authors.lastname: Bala
authors.forename: James
authors.initials: J
authors.lastname: Gomes
authors.forename: Nivedita Karmakar
authors.initials: NK
authors.lastname: Gohil
mesh_terms: D042783:Endothelial Cells / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000737:chemistry / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D000075462:Serum Albumin, Human; D013329:Structure-Activity Relationship; D000093423:Glycated Serum Albumin
chemical_list: D017127:Glycation End Products, Advanced; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic; D012709:Serum Albumin; C109748:advanced glycation end products-human serum albumin; D000075462:Serum Albumin, Human; D000093423:Glycated Serum Albumin