title: Symerythrin structures at atomic resolution and the origins of rubrerythrins and the ferritin-like superfamily.
abstract: Rubrerythrins are diiron-containing peroxidases that belong to the ferritin-like superfamily (FLSF). Here, we describe the structures of symerythrin, a novel rubrerythrin variant from the oxygenic phototroph Cyanophora paradoxa, at 1.20-1.40 Å resolution in three different states: diferric, azide-bound diferric and chemically reduced. The symerythrin metallocenter has a unique eighth ligating residue compared to rubrerythrin-an additional glutamate inserted into helix A of the four-helix bundle that resides on a π-helical segment. Otherwise, the diferric metallocenter structure is highly similar to that of characterized rubrerythrins. Azide binds the diferric center in a μ-1,1 orientation similar to how peroxide binds to diferric rubrerythrin. The structure of the diferrous metallocenter shows heterogeneity that we ascribe to the acidic pH of the crystals. In what we consider the neutral pH conformation, reduction causes a 2.0-Å shift in Fe1 and the toggling of a Glu to a His ligand, as seen with rubrerythrins. The function of symerythrin remains unknown, but preliminary tests showing oxidase and peroxidase activities and the similarities of its metallocenter to other rubrerythrins suggest similar functionalities between the two despite the additional ligating glutamate in symerythrin. Of particular interest is the high internal symmetry of symerythrin, which supports the notion that its core four-helix bundle was formed by the gene duplication and fusion of a two-helix peptide. Sequence comparisons with another family in the FLSF that also has notable internal symmetry provide compelling evidence that, contrary to previous assumptions, there have been multiple gene fusion events that have generated the single-chain FLSF fold.
authors.affiliation: Department of Biochemistry and Biophysics, 2011 Ag and Life Sciences Building, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Richard B
authors.initials: RB
authors.lastname: Cooley
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Arp
authors.forename: P Andrew
authors.initials: PA
authors.lastname: Karplus
mesh_terms: D000595:Amino Acid Sequence; D001386:Azides / Q000378:metabolism; D018360:Crystallography, X-Ray; D044424:Cyanophora / Q000737:chemistry / Q000201:enzymology*; D019143:Evolution, Molecular; D005293:Ferritins / Q000737:chemistry; D006422:Hemerythrin / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010545:Peroxides / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D012416:Rubredoxins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis
chemical_list: D001386:Azides; D006422:Hemerythrin; D010545:Peroxides; D012416:Rubredoxins; C055857:rubrerythrins; D005293:Ferritins