title: Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways.
abstract: The Chinese medicinal herb, Panax notoginseng, has long been used to treat bone fractures and Panax notoginseng saponins (PNS) could promote bone formation. Here, we investigated whether PNS could promote osteogenesis of bone marrow stromal cells (BMSCs) through modulating the MAPK signaling pathways, which are implicated in BMSC osteogenesis. We found that PNS markedly increased the mineralization of BMSCs by alizarin red S assays and stimulate alkaline phosphatase activity of these cells. Additionally, PNS significantly increased the mRNA levels of alkaline phosphatase, core-binding factor a1, and bone sialoprotein while decreasing PPARγ2 mRNA levels. Furthermore, inhibitors of ERK, PD98059, and p38, SB203580 inhibited the osteogenesis-potentiating effects by PNS. PNS stimulated the activation of ERK and p38 as evidenced by increased phosphorylation of these proteins, which was inhibited by PD98059 and SB203580. Our findings indicate that PNS could promote BMSC osteogenesis by activating the ERK and p38 signaling pathways.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital, Shantou University Medical College, Shantou, R.P. China.
authors.forename: Xue-Dong
authors.initials: XD
authors.lastname: Li
authors.forename: Zhao-Yong
authors.initials: ZY
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Chang
authors.forename: Dong-Xin
authors.initials: DX
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.forename: Yun-Guo
authors.initials: YG
authors.lastname: Wang
authors.forename: Jian-Kun
authors.initials: JK
authors.lastname: Xu
authors.forename: Dong-Yang
authors.initials: DY
authors.lastname: Huang
authors.forename: Shi-Xin
authors.initials: SX
authors.lastname: Du
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D053723:Panax notoginseng / Q000737:chemistry*; D010766:Phosphorylation; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D005419:Flavonoids; D007093:Imidazoles; D058574:Integrin-Binding Sialoprotein; D047495:PPAR gamma; D011725:Pyridines; D012503:Saponins; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one