title: Biogenesis of the unique 4',5'-dehydronucleoside of the uridyl peptide antibiotic pacidamycin.
abstract: The pacidamycins belong to a class of antimicrobial nucleoside antibiotics that act by inhibiting the clinically unexploited target translocase I, a key enzyme in peptidoglycan assembly. As with other nucleoside antibiotics, the pacidamycin 4',5'-dehydronucleoside portion is an essential pharmacophore. Here we show that the biosynthesis of the pacidamycin nucleoside in  Streptomyces coeruleorubidus  proceeds through three steps from uridine. The transformations involve oxidation of the 5'-alcohol by Pac11, transamination of the resulting aldehyde by Pac5, and dehydration by the Cupin-domain protein Pac13.
authors.affiliation: School of Chemistry, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.
authors.forename: Amany E
authors.initials: AE
authors.lastname: Ragab
authors.forename: Sabine
authors.initials: S
authors.lastname: Grüschow
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Tromans
authors.forename: Rebecca J M
authors.initials: RJ
authors.lastname: Goss
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry*; D005810:Multigene Family; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*; D014529:Uridine / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D014529:Uridine