title: Alterations of profibrinolytic receptor annexin A2 in pre-eclampsia: a possible role in placental thrombin formation.
abstract: BACKGROUND
  Deficient fibrinolytic activity due to alterations of profibrinolytic receptor annexin A2 has been shown to be related to increased thrombosis. We compared the expression of annexin A2 in pre-eclampsia with that in normal pregnancies and investigated its relationship to placental thrombin formation.
  
  
  METHODS
  Sixty patients with pre-eclampsia and 30 matched normal pregnant controls were included in the study. Expression of annexin A2 mRNA in placental tissues was analyzed using quantitative RT-PCR. Annexin A2 protein in placentas as well as blood was determined by western blot analysis and immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) was used to detect the presence of anti- annexin A2 antibodies in peripheral maternal blood.
  
  
  RESULTS
  The expression of annexin A2 mRNA in placentas was significantly decreased in pre-eclampsia compared to normal pregnancies. Levels of annexin A2 protein in placentas as well as in peripheral maternal blood were also significantly reduced in patients with pre-eclampsia compared with healthy pregnant women. High titers of anti- annexin A2 antibodies were more frequently detected in pre-eclampsia group. Decreased levels of annexin A2 expression and the presence of anti-annexin A2 antibodies were associated with increased placental thrombosis.
  
  
  CONCLUSIONS
  The down regulation of annexin A2 expression and the presence of anti-annexin A2 antibodies were correlated to PE and may increase placental thrombin formation.
authors.affiliation: Department of Obstetrics,The Second Hospital of Hebei Medical university, 215 Heping Xi Road, Shijiazhuang 050000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xin
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Huilan
authors.initials: H
authors.lastname: Wang
authors.forename: Shuhan
authors.initials: S
authors.lastname: Sun
mesh_terms: D000328:Adult; D017306:Annexin A2 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013917:Thrombin / Q000235:genetics / Q000378:metabolism*
chemical_list: D017306:Annexin A2; D012333:RNA, Messenger; D013917:Thrombin