title: Nicotine promotes proliferation and collagen synthesis of chondrocytes isolated from normal human and osteoarthritis patients.
abstract: The aims of the study were to show the direct effect of nicotine with different concentrations (0, 25, 50, and 100 ng/ml) on chondrocytes isolated from normal human and osteoarthritis patients, respectively. Microscopic observation was performed during the culture with an inverted microscope. Methyl thiazolyl tetrazolium (MTT) assay method was adopted to observe the influence of nicotine on the proliferation of chondrocytes, and real-time PCR and ELISA were used to assay the mRNA and protein expression of type II collagen and aggrecan, respectively. We discovered that the OA chondrocytes were similar to fibroblasts in shape and grow slower than normal chondrocytes. The proliferation of the two kinds of chondrocytes was increased in a concentration-dependent manner and in a time-dependent manner (P<0.05). Also, we found that the mRNA level of type II collagen were upregulated under 25-100 ng/ml nicotine doses both in the two kinds of chondrocytes compared with control. The expression of protein levels of type II collagen were synthesized in line with the increase in mRNA. No effect was observed on aggrecan synthesis with any nicotine dose. We concluded that nicotine has the same effect on both chondrocytes, obtained either from osteoarthritis patients or from normal human, and the positive effect of smoking in OA may relate to the alteration in metabolism of chondrocytes.
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, Xue Yuan Xi Road, Wenzhou, 325000, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Ying
authors.forename: Shaowen
authors.initials: S
authors.lastname: Cheng
authors.forename: Yue
authors.initials: Y
authors.lastname: Shen
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Cheng
authors.forename: Ferdinand
authors.initials: F
authors.lastname: An Rompis
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhongqin
authors.initials: Z
authors.lastname: Lin
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Dongquan
authors.initials: D
authors.lastname: Kou
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Xin Qiao
authors.initials: XQ
authors.lastname: Tian
authors.forename: Chuan Zhu
authors.initials: CZ
authors.lastname: Lu
mesh_terms: D016022:Case-Control Studies; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000096:biosynthesis*; D024043:Collagen Type II / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009538:Nicotine / Q000494:pharmacology*; D010003:Osteoarthritis / Q000473:pathology*; D012907:Smoking
chemical_list: D024043:Collagen Type II; D009538:Nicotine; D003094:Collagen