title: [Role of ERK in the hormesis induced by cadmium chloride in HEK293 cells].
abstract: OBJECTIVE
  To detect the role of ERK in the hormesis induced by cadmium chloride in HEK293 cells.
  
  
  METHODS
  HEK293 cells were treated with CdCl2 of 0, 0.0005, 0.005, 0.05, 0.5, 5, 50, 500 micromol/L for 12h and 24h. The ERK inhibitors (PD98059, U0126) and c-raf shRNA were added before the cell was treated with CdCl2. The proliferation viability was measured by thethiazolyl blue (MTT) and WST-8 assay. The level of the phosphorylated ERK and c-raf was examined with the western blot method. The level of c-fos, c-myc and c-raf mRNA was examined with RT-PCR.
  
  
  RESULTS
  CdCl2 stimulated cell proliferation at lower concentrations (0.05 and 0.5 micromol/L) but inhibit it at higher concentrations (50 and 500 micromol/L). The c-fos mRNA increased significantly at low concentrations (0.51 micromol/L) and the c-myc mRNA increased with CdCl2 concentration. Treated with 100 micromol/L ERK1/2 inhibitor and c-raf shRNA, the cell proliferation did not increase significantly at low concentrations (0.05 and 0.51 micromol/L), but still decreased at high concentrations (50 and 500 micromol/L).
  
  
  CONCLUSIONS
  CdCl2 can induce the hormesis of HEK293 cell proliferation. The c-fos of ERK pathway may be involved in the biphasic effect induced by CdCl2.
authors.affiliation: Department of Occupational Healthy, School of Public Health, Zhengzhou University, Zhengzhou 450001, China. haochangfu@126.com
authors.forename: Changfu
authors.initials: C
authors.lastname: Hao
authors.forename: Weidong
authors.initials: W
authors.lastname: Hao
mesh_terms: D002070:Butadienes / Q000494:pharmacology; D019256:Cadmium Chloride / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D005419:Flavonoids / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology
chemical_list: D002070:Butadienes; D005419:Flavonoids; D009570:Nitriles; C113580:U 0126; D048049:Extracellular Signal-Regulated MAP Kinases; D048052:Mitogen-Activated Protein Kinase 3; D019256:Cadmium Chloride; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one