title: Is ototopical nystatin ototoxic? A chinchilla model.
abstract: In this prospective controlled animal study, the authors investigated the potential ototoxic effects of ototopical application of nystatin through a tympanostomy tube, using their established chinchilla animal model. Each of the 10 animals used had ventilation tubes inserted in both ears; 1 ear was randomly assigned to receive nystatin suspension, whereas the other ear did not receive any medication, serving as control. Distortion product otoacoustic emissions (DPOAEs) were measured in each animal before application of nystatin and at 45 and 60 days after application. Each cochlea was also processed for scanning electron microscopy (SEM) at the end of the experiment. There was no significant difference in the DPOAEs and SEM appearances of the experimental and control ears over the 60-day period of the experiment. The authors conclude that transtympanic nystatin did not produce any long-term ototoxic effects detectable by DPOAEs or SEM.
authors.affiliation: Department of Pediatric Otolaryngology-Head and Neck Surgery, McGill University, Montreal Children's Hospital, Montreal, Quebec, Canada. sam.daniel@mcgill.ca
authors.forename: Sam J
authors.initials: SJ
authors.lastname: Daniel
authors.forename: Sofia
authors.initials: S
authors.lastname: Sahmkow
authors.forename: Olubunmi V
authors.initials: OV
authors.lastname: Akinpelu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002682:Chinchilla; D003051:Cochlea / Q000187:drug effects* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D008876:Middle Ear Ventilation; D009761:Nystatin / Q000494:pharmacology / Q000633:toxicity*; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects; D011897:Random Allocation; D012016:Reference Values; D012680:Sensitivity and Specificity; D014432:Tympanic Membrane / Q000187:drug effects*
chemical_list: D009761:Nystatin