title: Changes in the composition of an acid mine drainage microbial community upon successive transfers in medium containing low-grade copper sulfide.
abstract: A consortium of microorganisms from acid mine drainage samples was cultured in modified 9 K medium containing low-grade copper sulfide. The culture was maintained for sixty days and then transferred to fresh medium. This process was repeated three more times and a final consortium exhibiting a copper extraction rate of 89.3% was obtained. RFLP and microarrays analysis of 16S rRNA sequences retrieved from the consortia showed that Acidithiobacilluscaldus, Leptospirillumferriphilum, Sulfobacillus sp., Acidiphilium sp., and Sulfolobus spp. were represented in higher numbers in the consortia obtained in the copper-containing medium than in the original consortium. In contrast, a decrease in Acidithiobacillus ferrooxidans, Alicyclobacillus sp., Pseudomonas sp., and Sulfobacillus thermosulfidooxidans was observed. The abundance of genes related to sulfur metabolism from At. caldus and Sulfolobus spp., iron oxidation from Leptospirillum sp. and metal resistance from most of the detected microorganisms increased as the consortium was successively transferred into fresh medium.
authors.affiliation: School of Minerals Processesing and Bioengineering, Central South University, Changsha, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Yili
authors.initials: Y
authors.lastname: Liang
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Xian
authors.initials: X
authors.lastname: Fu
authors.forename: Ngom
authors.initials: N
authors.lastname: Baba
authors.forename: Weimin
authors.initials: W
authors.lastname: Zeng
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D000143:Acids / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000737:chemistry*; D005798:Genes, Bacterial; D016013:Likelihood Functions; D008906:Mining
chemical_list: D000143:Acids; D003300:Copper