title: Gene expression changes in femoral head necrosis of human bone tissue.
abstract: Osteonecrosis of the femoral head (ONFH) is the result of an interruption of the local circulation and the injury of vascular supply of bone. Multiple factors have been implicated in the development of the disease. However the mechanism of ischemia and necrosis in non-traumatic ONFH is not clear. The aim of our investigation was to identify genes that are differently expressed in ONFH vs. non-ONFH human bone and to describe the relationships between these genes using multivariate data analysis. Six bone tissue samples from ONFH male patients and 8 bone tissue samples from non-ONFH men were examined. The expression differences of selected 117 genes were analyzed by TaqMan probe-based quantitative real-time RT-PCR system. The significance test indicated marked differences in the expression of nine genes between ONFH and non-ONFH individuals. These altered genes code for collagen molecules, an extracellular matrix digesting metalloproteinase, a transcription factor, an adhesion molecule, and a growth factor. Canonical variates analysis demonstrated that ONFH and non-ONFH bone tissues can be distinguished by the multiple expression profile analysis of numerous genes controlled via canonical TGFB pathway as well as genes coding for extracellular matrix composing collagen type molecules. The markedly altered gene expression profile observed in the ONFH of human bone tissue may provide further insight into the pathogenetic process of osteonecrotic degeneration of bone.
authors.affiliation: 1st Department of Internal Medicine, Semmelweis University, Budapest, Hungary. betti@bel1.sote.hu
authors.forename: Bernadett
authors.initials: B
authors.lastname: Balla
authors.forename: Csaba
authors.initials: C
authors.lastname: Pintér
authors.forename: János P
authors.initials: JP
authors.lastname: Kósa
authors.forename: János
authors.initials: J
authors.lastname: Podani
authors.forename: István
authors.initials: I
authors.lastname: Takács
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Nagy
authors.forename: Gábor
authors.initials: G
authors.lastname: Speer
authors.forename: Balázs
authors.initials: B
authors.lastname: Horváth
authors.forename: László
authors.initials: L
authors.lastname: Korányi
authors.forename: Péter
authors.initials: P
authors.lastname: Lakatos
mesh_terms: D001842:Bone and Bones / Q000378:metabolism*; D005270:Femur Head / Q000473:pathology*; D005271:Femur Head Necrosis / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans