title: Optical trapping for the characterization of amyloid-beta aggregation kinetics.
abstract: Alzheimer's disease (AD) is marked by the accumulation of neuronal plaques from insoluble amyloid-beta (Aβ) peptides. Growing evidence for the role of Aβ oligomers in neuronal cell cytotoxicity and pathogenesis has prompted the development of novel techniques to better understand the early stages of aggregation. Near infrared (NIR) optical trapping was applied to characterize the early stages of Aβ aggregation in the presence of a β-sheet intercalating dye, Congo Red (CR), as the fluorescent marker. The integration of fluorescence analysis with NIR optical trapping has provided a new outlook into the first two hours of Aβ aggregation.
authors.affiliation: Dept. of Physical and Environmental Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON M1C 1A4, Canada.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Veloso
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Xin R
authors.initials: XR
authors.lastname: Cheng
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
authors.forename: Kagan
authors.initials: K
authors.lastname: Kerman
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D003224:Congo Red / Q000737:chemistry; D055664:Electrochemical Techniques; D007700:Kinetics; D007834:Lasers; D052898:Optical Tweezers; D010446:Peptide Fragments / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); D003224:Congo Red