title: A pyrene-based highly selective turn-on fluorescent sensor for copper(II) ion and its application in live cell imaging.
abstract: A new pyrene derivative (chemosensor 1) containing a picolinohydrazide moiety exhibits high selectivity for Cu(2+) ion detection in mixed aqueous media (CH(3)OH:H(2)O = 7:3). Significant fluorescence enhancement was observed with chemosensor 1 in the presence of Cu(2+). However, the metal ions Ag(+), Ca(2+), Cd(2+), Co(2+), Cu(2+), Fe(2+), Fe(3+), Hg(2+), K(+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), and Zn(2+) produced only minor changes in fluorescence for the system. The apparent association constant (K(a)) of Cu(2+) binding in chemosensor 1 was found to be 2.75*10(3) M(-1). The maximum fluorescence enhancement caused by Cu(2+) binding in chemosensor 1 was observed over the pH range 5-8. Moreover, by means of fluorescence microscopy experiments, it is demonstrated that 1 can be used as a fluorescent probe for detecting Cu(2+) in living cells.
authors.affiliation: Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China. spwu@mail.nctu.edu.tw
authors.forename: Shu-Pao
authors.initials: SP
authors.lastname: Wu
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Huang
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Liu
authors.forename: Peter Kun
authors.initials: PK
authors.lastname: Chung
mesh_terms: D002470:Cell Survival; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D003300:Copper / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D011721:Pyrenes; D003300:Copper; C030984:pyrene