title: A monoclonal antibody for in vivo detection of peroxisome-associated PTS1 receptor.
abstract: PEX5 is a key protein of the peroxisomal protein import machinery. This cycling receptor binds newly synthesized proteins with a peroxisomal targeting signal type 1 in the cytosol and directs them to the peroxisomal membrane. There, PEX5, together with its docking protein, forms a transient membrane-spanning channel that enables cargo-transport across the membrane. Through interaction with other multimeric membrane complexes, the receptor is released from the membrane back to the cytosol. Very little is known about the various conformational states of the receptor during its cycling. Here we report the generation and characterization of a mouse monoclonal antibody that recognizes in vivo primarily the membrane-associated form of the human PTS1 receptor.
authors.affiliation: Institute for Physiological Chemistry, Faculty of Medicine, Ruhr University of Bochum, Bochum, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Cizmowski
authors.forename: Martin
authors.initials: M
authors.lastname: Jung
authors.forename: Ralf
authors.initials: R
authors.lastname: Erdmann
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schliebs
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000918:Antibody Specificity; D002460:Cell Line; D018604:Epitope Mapping; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism*; D020033:Protein Isoforms / Q000276:immunology / Q000378:metabolism; D017434:Protein Structure, Tertiary; D018160:Receptors, Cytoplasmic and Nuclear / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; C000614264:PEX5 protein, human; D000074435:Peroxisome-Targeting Signal 1 Receptor; C000614265:Pex5 protein, mouse; D020033:Protein Isoforms; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins