title: Molecular approaches to analysing the microbial composition of raw milk and raw milk cheese.
abstract: The availability and application of culture-independent tools that enable a detailed investigation of the microbiota and microbial biodiversity of food systems has had a major impact on food microbiology. This review focuses on the application of DNA-based technologies, such as denaturing gradient gel electrophoresis (DGGE), temporal temperature gradient gel electrophoresis (TTGE), single stranded conformation polymorphisms (SSCP), the polymerase chain reaction (PCR) and others, to investigate the diversity, dynamics and identity of microbes in dairy products from raw milk. Here, we will highlight the benefits associated with culture-independent methods which include enhanced sensitivity, rapidity and the detection of microorganisms not previously associated with such products.
authors.affiliation: Teagasc, Moorepark Food Research Centre, Fermoy, Cork, Ireland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Quigley
authors.forename: Orla
authors.initials: O
authors.lastname: O'Sullivan
authors.forename: Tom P
authors.initials: TP
authors.lastname: Beresford
authors.forename: R Paul
authors.initials: RP
authors.lastname: Ross
authors.forename: Gerald F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: Paul D
authors.initials: PD
authors.lastname: Cotter
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D044822:Biodiversity; D002611:Cheese / Q000382:microbiology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005516:Food Microbiology; D006801:Humans; D008892:Milk / Q000737:chemistry / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D004269:DNA, Bacterial