title: FSCB phosphorylation in mouse spermatozoa capacitation.
abstract: It is generally accepted that spermatozoa capacitation is associated with protein kinase A-mediated tyrosine phosphorylation. In our previous study, we identified the fibrous sheath CABYR binding protein (FSCB), which was phosphorylated by PKA. However, the phosphorylation status of FSCB protein during spermatozoa capacitation should be further investigated. To this aim, in this study, we found that phosphorylation of this 270-kDa protein occurred as early as 1 min after mouse spermatozoa capacitation, which increased over time and remained stable after 60 min. Immunoprecipitation assays demonstrated that the tyrosine and Ser/Thr phosphorylation of FSCB occurred during spermatozoa capacitation. The extent of phosphorylation and was closely associated with the PKA activity and spermatozoa motility characteristics. FSCB phosphorylation could be induced by PKA agonist DB-cAMP, but was blocked by PKA antagonist H-89.Therefore, FSCB contributes to spermatozoa capacitation in a tyrosine-phosphorylated format, which may help in further elucidating the molecular mechanism of spermatozoa capacitation.
authors.affiliation: Department of Urology, Daping Hospital, The Third Military Medical University, Chongqing, China.
authors.forename: Shun-Li
authors.initials: SL
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Ni
authors.forename: Xiang-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Wen-Qian
authors.initials: WQ
authors.lastname: Huo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Tian
authors.forename: Ze-Min
authors.initials: ZM
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Tian
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Zheng
authors.forename: Feng-Shuo
authors.initials: FS
authors.lastname: Jin
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Li
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D008297:Male; D051379:Mice; D010766:Phosphorylation; D013075:Sperm Capacitation / Q000502:physiology*; D013081:Sperm Motility / Q000502:physiology
chemical_list: D002135:Calcium-Binding Proteins; C525804:FSCB protein, mouse; D017868:Cyclic AMP-Dependent Protein Kinases