title: Effects of type I protein kinase A modulation on the T cell distal pole complex.
abstract: The distal pole complex (DPC) assembles signalling proteins at the T cell pole opposite the immunological synapse (IS) and is thought to facilitate T cell activation by sequestering negative regulatory molecules away from the T cell receptor-proximal signalling machinery. Here, we report the translocation of type I protein kinase A (PKA) to the DPC in a fraction of T cells following activation and the localization of type I PKA with known components of the DPC. We propose that sequestration of type I PKA and concomitant loss of cAMP-mediated negative regulation at the IS may be necessary to allow full T cell activation. Moreover, composition of the DPC appears to be modulated by type I PKA activity, as the antagonist Rp-8-Br-cAMPS inhibited translocation of type I PKA and other DPC proteins.
authors.affiliation: The Biotechnology Centre, University of Oslo, Oslo, Norway.
authors.forename: R
authors.initials: R
authors.lastname: Mosenden
authors.forename: K
authors.initials: K
authors.lastname: Moltu
authors.forename: A
authors.initials: A
authors.lastname: Ruppelt
authors.forename: T
authors.initials: T
authors.lastname: Berge
authors.forename: K
authors.initials: K
authors.lastname: Taskén
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D054743:Cyclic AMP-Dependent Protein Kinase Type I / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D011485:Protein Binding; D013601:T-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000276:immunology*
chemical_list: D054743:Cyclic AMP-Dependent Protein Kinase Type I