title: Platelet-derived growth factor regulates breast cancer progression via β-catenin expression.
abstract: OBJECTIVE
  The knowledge on the association between platelet-derived growth factor (PDGF) signaling and epithelial cancers is scarce, although overexpression of PDGF and PDGF receptors has been reported in some human mesenchymal tumors. Thus, we studied the effect of PDGF on breast cancer cells in vitro and the distribution of PDGF in breast cancer tissues.
  
  
  METHODS
  The effect of PDGF-BB on breast cancer cells was assessed by Western blotting, immunofluorescence, WST and 5-bromo-2-deoxyuridine incorporation experiments. PDGF-B and β-catenin expression was investigated in breast cancer tissues by immunohistochemistry.
  
  
  RESULTS
  PDGF-BB induces β-catenin expression in breast cancer cells, and immunohistochemically the distribution of PDGF-B was similar to β-catenin in breast cancer cells. PDGF-B-positive cancer cells were more frequent in cases of ductal carcinoma in situ (87.5%) than invasive carcinoma (61.2%). In addition, PDGF-B staining was stronger in intraductal than invasive cancer cells. PDGF-BB tended to induce nuclear translocation of β-catenin, cell proliferation and DNA incorporation in MDA-MB231 cells, while these results were not found in MCF-7 cells.
  
  
  CONCLUSIONS
  Our results suggest that PDGF-BB regulates protein expression of β-catenin and is associated with cancer cell behavior.
authors.affiliation: Department of Molecular Pathology, Osaka University Graduate School of Medicine and Health Science, Osaka, Japan.
authors.forename: Yuhki
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Seiji
authors.initials: S
authors.lastname: Mori
authors.forename: Yoshinosuke
authors.initials: Y
authors.lastname: Hamada
authors.forename: Miki
authors.initials: M
authors.lastname: Hieda
authors.forename: Naomasa
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: Mohammed
authors.initials: M
authors.lastname: Shaker
authors.forename: Yu
authors.initials: Y
authors.lastname: Tao
authors.forename: Katsuhide
authors.initials: K
authors.lastname: Yoshidome
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
mesh_terms: D000077214:Becaplermin; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D051176:beta Catenin / Q000378:metabolism*
chemical_list: C495270:CTNNB1 protein, human; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D051176:beta Catenin; D000077214:Becaplermin