title: A Campylobacter jejuni Dps homolog has a role in intracellular survival and in the development of campylobacterosis in neonate piglets.
abstract: Iron acquisition is an absolute requirement by most microorganisms for host survival. In this work, we investigated the Campylobacter jejuni iron binding Dps protein for a potential role in virulence. In vitro assays using J774A.1 macrophage-like cells demonstrated a 2.5 log reduction in C. jejuni survival of the Dps mutant and a reduction of four logs in invasion of HEp-2 epithelial cells compared to the wild-type strain. To examine the role of the dps gene in host pathogenesis, the piglet model was used in C. jejuni challenge studies. In vivo inoculation studies of newborn piglets with wild-type C. jejuni demonstrated an 11-fold upregulation of the dps gene and intestinal lesion production typical of campylobacteriosis in humans. In contrast, piglets inoculated with the dps mutant were not colonized and remained normal throughout the study period. Mucosal lesion production was restored in piglets inoculated with the complemented Dps mutant strain. Based on these results, we conclude that the C. jejuni Dps homolog is a virulence factor in the production of campylobacteriosis, and warrants further investigation.
authors.affiliation: Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona 85721, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Theoret
authors.forename: Kerry K
authors.initials: KK
authors.lastname: Cooper
authors.forename: Robert D
authors.initials: RD
authors.lastname: Glock
authors.forename: Lynn A
authors.initials: LA
authors.lastname: Joens
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D057138:Bacterial Shedding; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D005816:Genetic Complementation Test; D033862:Iron-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000382:microbiology; D050296:Microbial Viability; D009154:Mutation; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D013997:Time Factors; D015533:Transcriptional Activation; D015854:Up-Regulation / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C083911:DPS protein, Bacteria; D033862:Iron-Binding Proteins; D037521:Virulence Factors