title: Yos9, a control protein for misfolded glycosylated and non-glycosylated proteins in ERAD.
abstract: The endoplasmic reticulum (ER) is responsible for folding and delivery of secretory proteins to their site of action. One major modification proteins undergo in this organelle is N-glycosylation. Proteins that cannot fold properly will be directed to a process known as endoplasmic reticulum associated degradation (ERAD). Processing of N-glycans generates a signal for ERAD. The lectin Yos9 recognizes the N-glycan signal of misfolded proteins and acts as a gatekeeper for the delivery of these substrates to the cytoplasm for degradation. Presence of Yos9 accelerates degradation of the glycosylated model ERAD substrate CPY∗. Here we show that Yos9 has also a control function in degradation of the unglycosylated ERAD substrate CPY∗0000. It decelerates its degradation rate.
authors.affiliation: Institut für Biochemie der Universität Stuttgart, Stuttgart, Germany.
authors.forename: Elena Martinez
authors.initials: EM
authors.lastname: Benitez
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stolz
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Wolf
mesh_terms: D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D060746:Endoplasmic Reticulum-Associated Degradation / Q000502:physiology*; D006031:Glycosylation; D008361:Mannosidases / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; C495027:Yos9 protein, S cerevisiae; C434527:MNL1 protein, S cerevisiae; D008361:Mannosidases; D002268:Carboxypeptidases; C520242:CPY protein, S cerevisiae