title: Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer.
abstract: We developed a new single nucleotide polymorphism (SNP) genotyping method based on single-molecule multi-color fluorescence resonance energy transfer (FRET). We demonstrated that this new method uses less than 1 fmol of sample and is also highly quantitative with a detection level of 1% or lower in the minor allele fraction.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 151-747, Korea.
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Koh
authors.forename: Kyu Young
authors.initials: KY
authors.lastname: Han
authors.forename: Jiwon
authors.initials: J
authors.lastname: Jung
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kim
mesh_terms: D003116:Color; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods*; D007834:Lasers; D016014:Linear Models; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*