title: In vitro analyses of endothelial cell permeability.
abstract: Endothelial cells lining the vessels of the vasculature and the cell-cell junctions, which join them, -provide the primary barrier to the passage of fluids, immune cells, and macromolecules between the bloodstream and the tissues. Appropriate and dynamic regulation of this barrier is required during normal -physiological processes; however, if not tightly controlled, increased permeability of the endothelium can also contribute to many pathological situations, including chronic inflammatory diseases and edema. The development of in vitro methods to study endothelial barrier function has been key in the identification of molecular mechanisms underlying many of these disease states. In this chapter, we describe three complementary approaches to measure endothelial monolayer permeability and barrier function in vitro.
authors.affiliation: Department of Cell and Developmental Biology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Monaghan-Benson
authors.forename: Erika S
authors.initials: ES
authors.lastname: Wittchen
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D003911:Dextrans / Q000032:analysis; D015194:Diffusion Chambers, Culture; D017097:Electric Impedance; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004727:Endothelium / Q000166:cytology / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000032:analysis; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D011199:Potentiometry / Q000379:methods*; D013873:Thionucleotides / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D000242:Cyclic AMP; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate; D013873:Thionucleotides; D013917:Thrombin; D042461:Vascular Endothelial Growth Factor A; D000099152:Cadherin 5; C467484:VEGFA protein, human; C015219:fluorescein isothiocyanate dextran; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP