title: Isolation and characterization of a novel agarase-producing Pseudoalteromonas spp. bacterium from the guts of spiny turban shells.
abstract: An agar-degrading bacterium was isolated from the guts of spiny turban shells. It was identified as a Pseudoalteromonas species and named Pseudoalteromonas sp. JYBCL 1. The viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. The agarase produced by the isolate had optimal activities at 35 degrees C and pH 7. The enzyme had extremely strong resistance to ionic stress compared with other known agarases. Its molecular mass was estimated at about 60 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The agarase could saccharify Gelidium amansii directly, with an efficiency about half that compared with agar saccharification.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Changkyou
authors.initials: C
authors.lastname: Jung
authors.forename: Jinwon
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D049851:Gastropoda / Q000382:microbiology*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D031161:Pseudoalteromonas / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C019624:agarase