title: Comparison of liposome-polycation-DNA(LPD) and monophosphoryl lipid A(MPL) adjuvant formulations in BALB/c mice models.
abstract: It is of fundamental importance to use an appropriate adjuvant to generate a potent immune response for immunotherapy. In this study, we had a comparative investigation on the effectiveness of two adjuvant formulations, liposome-polycation-DNA (LPD) and monophosphoryl lipid A(MPL) in combination with a truncated peptide of bFGF(tbFGF) as antigen. LPD/tbFGF induced continuously increasing antibodies expression during the whole immunization period. In contrast, the level of antibodies was variable in MPL/tbFGF-immunized mice, MPL/tbFGF elicited potent antibodies response in the early-phase of immunization (during the first 3 immunizations), but the later immunizations did not produce a significant increase in the level of antibodies. Evaluation of IFN-γ and IL-4 responses revealed that both LPD/tbFGF and MPL/tbFGF demonstrated generation of higher level of IFN-γ, whereas no significant increase in IL-4 levels was detected in the two groups. In addition, histological analysis exhibited obvious germinal centers in the spleen tissues of LPD/tbFGF mice. The data suggested that LPD would be a promising long-effective adjuvant due to its potent and persistent immunostimulation and MPL could play an appropriate role in short-acting immunization.
authors.affiliation: Gansu College of Traditional Chinese Medicine, Lanzhou, Gansu, China. pzhang@gmail.com
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Cui
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D016207:Cytokines / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000276:immunology; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry; D008050:Lipid A / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011073:Polyamines / Q000737:chemistry / Q000276:immunology; D000071228:Polyelectrolytes; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology / Q000633:toxicity
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D016207:Cytokines; D004247:DNA; D016222:Fibroblast Growth Factor 2; D008050:Lipid A; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; D014614:Vaccines, Synthetic; C408982:CPG-oligonucleotide; C048436:monophosphoryl lipid A