title: Immobilization of horseradish peroxidase on silane-modified ceramics and their properties: potential for oily wastewater treatment.
abstract: Horseradish peroxidase (HRP) is proved being effective in eliminating oil from aqueous solutions, but the elimination is expensive because free HRP can not be reused. In present work, HRP was successfully immobilized on cordierite porous ceramics support with a novel method of N-beta-aminoethyl-gamma-aminopropyl-trimethoxysilane modification and glutaraldehyde activation. Under the optimized immobilized conditions, the actual immobilized HRP was 1.16 mg/g support, the activity of the immobilized HRP could reach as high as 1379.4 U/g support. Experiment results showed that the properties of storage stability, acid-base stability and the tolerance to the pH fluctuation of the immobilized HRP were better than those of the free HRP. The operation stability of the immobilized HRP was also good. The immobilized HRP is suitable for the oily wastewater treatment because of its reusability proved in this work.
authors.affiliation: College of Chemical Engineering Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China. liuw@mail.buct.edu.cn
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: W C
authors.initials: WC
authors.lastname: Wang
authors.forename: H S
authors.initials: HS
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhou
mesh_terms: D002516:Ceramics / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005976:Glutaral / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D009821:Oils / Q000737:chemistry / Q000378:metabolism*; D012821:Silanes / Q000737:chemistry*; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D009821:Oils; D012821:Silanes; D014874:Water Pollutants, Chemical; D006735:Horseradish Peroxidase; D005976:Glutaral