title: Identification of new aberrantly expressed miRNAs in intestinal-type             gastric cancer and its clinical significance.
abstract: miRNAs are small 19 to 22 nucleotide sequences of RNA that negatively regulate             gene expression. miRNA expression profiles may become useful biomarkers for diagnostics,             prognosis and prediction of response to treat, and it could be a powerful tool             for cancer prevention and therapeutics. Several miRNA expression profiles of miRNAs             in gastric cancer have been reported, but these studies screened only few miRNAs             and samples used in experiments include several different subtypes of gastric             cancers, which decrease the sensitivity to identify new aberrant miRNAs. In this             study, a miRNA expression profile was identified by miRCURY LNA Array (v.14.0)             between intestinal-type gastric cancers and normal tissues. Forty miRNA precursors             were up-regulated and thirty-six miRNA were down-regulated in intestinal-type             gastric cancers (p<0.01). Sixteen new miRNAs were found in intestinal-type             gastric cancers. Seventeen new miRNAs were found in intestinal-type gastric cancers.             miR-145, miR-27a, miR-494 are differently expressed between intestinal-type and             diffuse-type gastric cancers. miR-32, miR-182 and miR-143 dysregulated expression             levels are related with different pathological stages of intestinal-type gastric             cancers (p<0.01). Taken together, aberrantly expressed miRNAs may offer new             clues to tumorigenesis of gastric cancers. miR-32, miR-182 and miR-143 may be             potential diagnostic biomarkers for intestinal-type gastric cancers.
authors.affiliation: Department of Gastroenterology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.forename: Feijun
authors.initials: F
authors.lastname: Luo
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Meihua
authors.initials: M
authors.lastname: Xu
authors.forename: Deyun
authors.initials: D
authors.lastname: Feng
authors.forename: Guiying
authors.initials: G
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D016000:Cluster Analysis; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009367:Neoplasm Staging; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C542359:MIRN143 microRNA, human; C568529:MIRN32 microRNA, human; C548912:MIRN483 microRNA, human; D035683:MicroRNAs; C538984:Mirn182 microRNA, human