title: MiR-138 suppresses expression of hypoxia-inducible factor 1α (HIF-1α) in clear cell renal cell carcinoma 786-O cells.
abstract: OBJECTIVE
  Hypoxia-inducible factor-1alpha (HIF-1a) is widely considered to be one of the key regulators in cancer cells. Here, we investigated a microRNA regulating expression of HIF-1a and explored its functions in clear cell renal cell carcinoma (ccRCC) cells.
  
  
  METHODS
  Western blot and reporter assays were used to assess HIF-1a as a direct target of miR-138. The effects of miR-138 or si-HIF-1a on ccRCC 786-O cells were also estimated by apoptosis analysis and cell migration assay.
  
  
  RESULTS
  The data showed HIF-1a to be one target of miR-138. Futhermore, inhibition of the expression of HIF-1a with specific siRNA or miR-138 could increase apoptosis and reduce the migration of 786-O cells.
  
  
  CONCLUSIONS
  miR-138 could inhibit the expression of HIF-1a and regulate the apoptosis and migration of ccRCC cells.
authors.affiliation: Department of Urology, Clinical Division of Surgery, Chinese PLA General Hospital, Beijing, China. songtao6669@sina.cn
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Chunyang
authors.initials: C
authors.lastname: Wang
authors.forename: Yiguang
authors.initials: Y
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.forename: Jiangping
authors.initials: J
authors.lastname: Gao
authors.forename: Baofa
authors.initials: B
authors.lastname: Hong
mesh_terms: D017209:Apoptosis; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C546110:MIRN138 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering