title: Separation and purification of ginsenoside Re from ginseng bud by selective adsorption of active carbon and preparative high-performance liquid chromatography.
abstract: Ginsenoside Re (G-Re) is a major ingredient of the ginseng bud. A novel and rapid method to isolate and purify G-Re from ginseng buds was established. The procedure involves solvent extraction of ginseng bud powder, then pre-purification with an active carbon column and purification by preparative HPLC (prep-HPLC). Active carbon, which can selectively adsorb other kinds of ginsenosides except G-Re, was used as a new media to pre-purify G-Re in this study. In addition, we describe the development and optimisation of prep-HPLC parameters for G-Re purification. Compared to other types of high-purity G-Re preparation methods, this method is efficient, economical, waste-conscious and has the potential to maximise.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
authors.forename: Pengqun
authors.initials: P
authors.lastname: Kuang
authors.forename: Guan
authors.initials: G
authors.lastname: Wang
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000302:isolation & purification*; D005894:Panax / Q000737:chemistry*
chemical_list: D036145:Ginsenosides; C049864:ginsenoside Re