title: Enhanced IL-10 production by CD4+ T cells primed in IL-15Rα-deficient mice.
abstract: In this study, we investigated the functional outcomes of CD4(+)  T cells primed in the absence of IL-15 transpresentation. Compared with their WT counterparts primed in WT mice, IL-15Rα KO CD4(+)  T cells primed in KO mice were found to exclusively overproduce IL-10 upon in vitro restimulation(.)  The comparable expression of IL-4 and Foxp3 in CD4(+)  T cells primed in the WT and IL-15Rα KO mice indicated that this was neither due to T(H) 2- nor Treg cell-differentiation. IL-10 overproduction was also observed when OVA-specific TCR transgenic CD4(+)  T (OT-II) cells were primed in KO mice, excluding an intrinsic deficiency of KO CD4(+)  T cells. To investigate the WT and KO microenvironment, DCs from both WT and IL-15Rα KO mice were compared. DCs from both backgrounds were indistinguishable in their steady-state survival and in their expression of MHC class II and costimulatory molecules CD80, CD86, and CD40. However, IL-15Rα KO DCs primed OT-II cells in vitro to produce higher levels of IL-10 upon their restimulation. Additionally, IL-15Rα KO DCs produced significantly more IL-10 upon activation, and IL-10 neutralization during DC-mediated in vitro priming abolished IL-10 overproduction by CD4(+)  T cells. Thus, IL-15Rα KO DCs provide an IL-10-enriched environment that preferentially primes CD4(+)  T cells for more IL-10 production, highlighting a regulatory role for IL-15 transpresentation in CD4(+)  T-cell priming.
authors.affiliation: Department of Microbiology and Immunology, Chang Gung University,Taoyuan, Taiwan, ROC.
authors.forename: Kai-Ping N
authors.initials: KP
authors.lastname: Chow
authors.forename: Jan-Mou
authors.initials: JM
authors.lastname: Lee
authors.forename: Jian-Tai
authors.initials: JT
authors.lastname: Qiu
authors.forename: Shuen-Kuei
authors.initials: SK
authors.lastname: Liao
authors.forename: Shih-Chieh
authors.initials: SC
authors.lastname: Lin
authors.forename: Shuo-Lun
authors.initials: SL
authors.lastname: Hsu
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Wu
authors.forename: Yen-Fu
authors.initials: YF
authors.lastname: Lin
authors.forename: Tzong-Shoon
authors.initials: TS
authors.lastname: Wu
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002469:Cell Separation; D005434:Flow Cytometry; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D053720:Interleukin-15 Receptor alpha Subunit / Q000172:deficiency / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: C556062:IL10 protein, mouse; D053720:Interleukin-15 Receptor alpha Subunit; D016753:Interleukin-10