title: [Validation of a HPLC method for ochratoxin A determination].
abstract: Ochratoxin A is a mycotoxin produced by various species of Aspergillus and Penicillium. Ochratoxin A has been detected in cereals and cereal products, coffee beans, beer, wine, spices, pig's kidney and cow's milk. For ochratoxin A, a HPLC method was developed and validated. Ochratoxin A was determined by RP-HPLC, using a liquid chromatograph type HP 1090 Series II, equiped with a fluorescence detector. The analysis was performed with a Phenomenex column, type Luna C18(2) 100A (150 x 4.6 mm; 5 microm) with a mobile phase consisting of a mixture of acetonitrile/water/acid acetic (99/99/2), a flow of 0.7 mL/min. For detection, the wavelenght of excitation was 228 nm and wavelenght of emision was 423 nm. The calibration graph was linear in 6.25-50 ng/mL concentration range (r2 = 0,9991). The detection limits was 1.6 ng/mL and the quantification limit was 4.9 ng/mL. The method precision (RSD = 2.4975%) and the accuracy (recovery was 100.1%) were studied. The HPLC method was applyed for ochratoxin A from food samples with good results.
authors.affiliation: Universitatea de Medicină şi Farmacie Gr. T. Popa Iaşi, Facultatea de Farmacie, Disciplina Farmacodinamie.
authors.forename: Delia
authors.initials: D
authors.lastname: Bulea
authors.forename: A F
authors.initials: AF
authors.lastname: Spac
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D002273:Carcinogens; D009793:Ochratoxins; C025589:ochratoxin A