title: Hd86, the Bm86 tick protein ortholog in Hyalomma scupense (syn. H. detritum): expression in Pichia pastoris and analysis of nucleotides and amino acids sequences variations prior to vaccination trials.
abstract: The genus Hyalomma includes the most frequent tick species infesting livestock in North Africa, one of these species, Hyalomma scupense (syn. H. detritum) is particularly important due to its role in the transmission of tropical theileriosis to cattle (Theileria annulata infection). We have cloned and characterized the orthologs of the Bm86 gene from H. scupense strains collected over Tunisia in 2006 and 2009. The recombinant protein rHd86 was expressed in Pichia pastoris for vaccination purpose using a transcript from the 2006 strain. The rHd86 was then purified from the yeast culture supernatant by a filtration and a size exclusion process. It was recognized by specific anti-Bm86 antisera. An important extent of inter-specific diversity ranging from 35 to 40% was recorded between Hd86 and Bm86/Bm95 proteins whilst a very limited level of intra-specific diversity (1.7%) occurred between the Hd86 vaccine candidate protein and its homologues from H. scupense strains collected in 2009. These results emphasise the need for assessing the efficacy against H. scupense and others important cattle Hyalomma species in Tunisia of our Hd86 vaccine candidate alongside with a Bm86 vaccine.
authors.affiliation: Laboratoire de Parasitologie Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisia.
authors.forename: Mourad
authors.initials: M
authors.lastname: Ben Said
authors.forename: Yousr
authors.initials: Y
authors.lastname: Galai
authors.forename: Mario
authors.initials: M
authors.lastname: Canales
authors.forename: Ard Menzo
authors.initials: AM
authors.lastname: Nijhof
authors.forename: Moez
authors.initials: M
authors.lastname: Mhadhbi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jedidi
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente
authors.forename: Mohamed Aziz
authors.initials: MA
authors.lastname: Darghouth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D015870:Gene Expression; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D026863:Ixodidae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016796:Theileria annulata; D013801:Theileriasis / Q000517:prevention & control; D014416:Tunisia; D014612:Vaccines / Q000378:metabolism*
chemical_list: D000941:Antigens; D060829:Arthropod Proteins; D006023:Glycoproteins; D011994:Recombinant Proteins; D014612:Vaccines