title: The first simultaneous study of the prevalence of Neisseria gonorrhoeae and Chlamydia trachomatis infections by polymerase chain reaction and standard methods.
abstract: BACKGROUND
  Neisseria gonorrhoeae and Chlamydia trachomatis are important sexually transmissible pathogens. The aim of the present study was to determine their prevalence in symptomatic and asymptomatic people.
  
  
  METHODS
  Polymerase chain reaction (PCR) and standard methods were tested for 413 specimens.
  
  
  RESULTS
  The registered prevalence with PCR was N. gonorrhoeae 2.66% and C. trachomatis 0.73%. One man showed co-infection. Standard methods demonstrated lower sensitivity in finding N. gonorrhoeae and C. trachomatis in comparison with the PCR.
  
  
  CONCLUSIONS
  This is the first simultaneous study in Bulgaria for detection of N. gonorrhoeae and C. trachomatis by PCR and standard methods. N. gonorrhoeae was found to be a more frequent infection. Further investigations are needed.
authors.forename: Vessela V
authors.initials: VV
authors.lastname: Ouzounova-Raykova
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Tibi
authors.forename: Ivan G
authors.initials: IG
authors.lastname: Mitov
mesh_terms: D002031:Bulgaria; D002690:Chlamydia Infections / Q000175:diagnosis / Q000453:epidemiology*; D002692:Chlamydia trachomatis; D015897:Comorbidity; D003430:Cross-Sectional Studies; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis / Q000453:epidemiology*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data*; D011237:Predictive Value of Tests; D014600:Utilization Review