title: Protein kinase C regulates ezrin-radixin-moesin phosphorylation in canine osteosarcoma cells.
abstract: The development of metastasis is the most significant cause of death for both canine and human patients with osteosarcoma (OS). Ezrin has been associated with tumour progression and metastasis in human, canine and murine OS. Ezrin activation is dynamically regulated by protein kinase C (PKC) during metastatic progression in human and murine OS. To include the dog in the development of therapeutics that target ezrin biology, we characterized four new canine OS cell lines and confirmed the relationship between PKC and ezrin in these cells. Three of four cell lines formed tumours in mice that were histologically consistent with OS. All cell lines were markedly aneuploid and expressed ezrin and PKC. Finally, both ezrin phosphorylation and cell migration were inhibited using a PKC inhibitor. These data suggest that an association between PKC-mediated activation of ezrin and the metastatic phenotype in canine OS cells.
authors.affiliation: Tumor and Metastasis Biology Section, Pediatric Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. hongsu@mail.nih.gov
authors.forename: S-H
authors.initials: SH
authors.lastname: Hong
authors.forename: T
authors.initials: T
authors.lastname: Osborne
authors.forename: L
authors.initials: L
authors.lastname: Ren
authors.forename: J
authors.initials: J
authors.lastname: Briggs
authors.forename: C
authors.initials: C
authors.lastname: Mazcko
authors.forename: S S
authors.initials: SS
authors.lastname: Burkett
authors.forename: C
authors.initials: C
authors.lastname: Khanna
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism / Q000662:veterinary*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D016513:Mice, SCID; D008840:Microfilament Proteins / Q000378:metabolism; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000378:metabolism / Q000662:veterinary*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D012376:Rodent Diseases / Q000378:metabolism*; D015398:Signal Transduction; D014945:Wound Healing; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D011493:Protein Kinase C; D000099149:Ezrin; C071058:moesin; C071776:radixin