title: [Genetic characterization of poliovirus isolates from environmental sewage surveillance in Shandong, 2010].
abstract: To investigate the genetic characteristics of poliovirus isolates from environmental sewage surveillance in Shandong province, we collected sewage samples in Jinan and Linyi City. Serotyping and VP1/ 3D sequencing were performed on polioviruses isolated from the concentrated sewage samples, and VP1 mutation and recombination were analyzed. Thirty-two of sewage samples were collected, and polioviruses were detected in 10 of the samples with a positive rate of 31.3%. Eighteen Sabin strains were isolated including three type 1, nine type 2, and six type 3 polioviruses, and the number of nucleotide substitutions in VP1 coding region varied from 0 to 4. Recombination was found in three Sabin 2 and four Sabin 3 polioviruses. Analysis of neurovirulence sites of VP1 revealed that one Sabin 1 vaccine strain had a nucleotide change of A to G at nt 2749, one Sabin 2 strain had a nucleotide change of A to G at nt 2908, three Sabin 2 strains had a nucleotide change of U to C at nt 2909, and all six Sabin 3 strains had a nucleotide change of C to U at nt 2493. Poliovirus vaccine strains could be isolated from environmental sewage with a high rate of gene recombination and back mutation of neuvirulence-associated sites. None of wild-type poliovirus or vaccine-derived poliovirus was detected.
authors.affiliation: Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China. zhangyan204@sina.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sheng-Xiang
authors.initials: SX
authors.lastname: Ji
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ze-Xin
authors.initials: ZX
authors.lastname: Tao
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Shuang-Li
authors.initials: SL
authors.lastname: Zhu
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Ji
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Feng
authors.forename: Yoshida
authors.initials: Y
authors.lastname: Hiromu
authors.forename: Ai-Qiang
authors.initials: AQ
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002681:China; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011051:Poliomyelitis / Q000517:prevention & control* / Q000821:virology; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification*; D011159:Population Surveillance; D012722:Sewage / Q000821:virology*
chemical_list: D012722:Sewage