title: Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram.
abstract: Sensitivity of baker's yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demonstrated by increased superoxide production and decrease of cellular glutathione content. Anoxic atmosphere and hydrophilic antioxidants (ascorbate, glutathione, dithiothreitol, cysteine, and N-acetylcysteine) ameliorated DSF toxicity to yeast indicating that oxidative stress plays a critical role in the cellular action of DSF.
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, ul. Zelwerowicza 4, 35-601 Rzeszow, Poland. mkwolek@univ.rzeszow.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kwolek-Mirek
authors.forename: Renata
authors.initials: R
authors.lastname: Zadrag-Tecza
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004221:Disulfiram / Q000633:toxicity*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000172:deficiency / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid; D004221:Disulfiram