title: Vasodilator-stimulated phosphoprotein regulates osteosarcoma cell migration.
abstract: Vasodilator-stimulated phosphoprotein (VASP) has been reported to play an             important role in the process of cell migration and tumor metastasis. However,             to date, no study has examined VASP expression and its function in osteocarcoma             cells. In this study, we analyzed the effect of VASP on osteosarcoma cell migration             and the signal transduction pathways involved. We used two osteosarcoma cell strains             (Mg-63 and Saos-2 cells) with different metastatic potential. Silencing of VASP             gene expression was carried out using RNA interference in these cells. Knockdown             of expression at the transcriptional or translational level was determined by             RT-PCR or western blot analysis, respectively. The metastatic potential of the             tumor cells was determined by a wound healing migration assay. VASP mRNA expression             was also determined in 30 human osteosarcoma samples. Furthermore, Rac1 was determined             as a regulator of VASP function. RT-PCR and western blotting showed that Mg-63             cells had a significantly higher VASP expression at both the transcriptional and             translational levels compared to Saos-2 cells. The wound healing assay revealed             that Mg-63 cells had more migratory potential compared to Saos-2 cells. The effect             was found to be reversible when VASP was knocked down by siRNA in Mg-63 cells.             Specimens from human patients with metastases had higher VASP expression compared             to specimens of patients without metastases. Knockdown of Rac1 resulted in inhibition             of VASP expression in sarcoma cells. These results suggest that VASP protein regulates             osteosarcoma cell migration and metastasis. Rac1 and VASP interaction may be a             potential target for osteo-sarcoma treatment.
authors.affiliation: Department of Orthopedics, Zhongnan Hospital of Wuhan University, and Department of Pathology and Pathophysiology, Medical School of Wuhan University, Wuhan, Hubei 430071, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Wei
authors.forename: Aixi
authors.initials: A
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Baiwen
authors.initials: B
authors.lastname: Qi
authors.forename: Ke
authors.initials: K
authors.lastname: Su
authors.forename: Xiang
authors.initials: X
authors.lastname: Hu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002648:Child; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012516:Osteosarcoma / Q000378:metabolism* / Q000556:secondary; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D034622:RNA Interference; D015398:Signal Transduction; D014158:Transcription, Genetic; D055815:Young Adult; D020830:rac1 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism; D000098662:Vasodilator-Stimulated Phosphoprotein
chemical_list: D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D010750:Phosphoproteins; C107953:RAC1 protein, human; D000098662:Vasodilator-Stimulated Phosphoprotein; D020830:rac1 GTP-Binding Protein