title: Development of a highly sensitive and specific immunoassay for determining chrysoidine, a banned dye, in soybean milk film.
abstract: A highly specific and sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA）was developed for the first time for the detection of chrysoidine, a dye banned in soybean milk film. Two haptens with different spacer arms were synthesized to produce antibodies. Both homologous and heterologous immunoassay formats were compared to enhance the icELISA sensitivity. The heterologous icELISA exhibited better performance, with an IC(50) (50% inhibitory concentration) of 0.33 ng/mL, a limit of detection (LOD, 10% inhibitory concentration) of 0.04 ng/mL, and a limit of quantitation (LOQ, 20%-80% inhibitory concentration) from 0.09 to 4.9 ng/mL. The developed icELISA was high sensitive and specific, and was applied to determine chrysoidine in fortified soybean milk film samples. The results were in good agreement with that obtained by high-performance liquid chromatography (HPLC) analyses.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, China. hongtao@scau.edu.cn
authors.forename: Hongtao
authors.initials: H
authors.lastname: Lei
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Lijun
authors.initials: L
authors.lastname: Song
authors.forename: Yudong
authors.initials: Y
authors.lastname: Shen
authors.forename: Simon A
authors.initials: SA
authors.lastname: Haughey
authors.forename: Haoxian
authors.initials: H
authors.lastname: Guo
authors.forename: Jinyi
authors.initials: J
authors.lastname: Yang
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Xu
authors.forename: Yueming
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001667:Binding, Competitive; D002138:Calibration; D002273:Carcinogens / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004655:Emulsions / Q000032:analysis* / Q000737:chemistry; D004785:Environmental Pollutants / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005506:Food Contamination / Q000032:analysis* / Q000517:prevention & control; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D006801:Humans; D057230:Limit of Detection; D011817:Rabbits; D013025:Glycine max / Q000737:chemistry*; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000906:Antibodies; D002273:Carcinogens; D004655:Emulsions; D004785:Environmental Pollutants; D000939:Epitopes; D006241:Haptens; C005562:chrysoidine; D010128:p-Aminoazobenzene