title: Quantitative proteomics by amino acid labeling in C. elegans.
abstract: We demonstrate labeling of Caenorhabditis elegans with heavy isotope-labeled lysine by feeding them with heavy isotope-labeled Escherichia coli. Using heavy isotope-labeled worms and quantitative proteomics methods, we identified several proteins that are regulated in response to loss or RNAi-mediated knockdown of the nuclear hormone receptor 49 in C. elegans. The combined use of quantitative proteomics and selective gene knockdown is a powerful tool for C. elegans biology.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
authors.forename: Julius
authors.initials: J
authors.lastname: Fredens
authors.forename: Kasper
authors.initials: K
authors.lastname: Engholm-Keller
authors.forename: Anders
authors.initials: A
authors.lastname: Giessing
authors.forename: Dennis
authors.initials: D
authors.lastname: Pultz
authors.forename: Martin Røssel
authors.initials: MR
authors.lastname: Larsen
authors.forename: Peter
authors.initials: P
authors.lastname: Højrup
authors.forename: Jakob
authors.initials: J
authors.lastname: Møller-Jensen
authors.forename: Nils J
authors.initials: NJ
authors.lastname: Færgeman
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000032:analysis* / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D007553:Isotope Labeling; D008239:Lysine / Q000737:chemistry*; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics
chemical_list: D029742:Caenorhabditis elegans Proteins; D020543:Proteome; D008239:Lysine