title: Suppression of MED19 expression by shRNA induces inhibition of cell proliferation and tumorigenesis in human prostate cancer cells.
abstract: MED19 is a member of the Mediator that plays a key role in the activation and repression of signal transduction or the regulation of transcription in carcinomas. To tested the functional role of MED19 in human prostate cancer, we downregulated MED19 expression in prostate cancer cells (PC-3 and DU145) by lentivirus-mediated short hairpin (shRNA), and analyzed the effect of inhibition of MED19 on prostate cancer cell proliferation and tumorigenesis. The in vitro prostate cancer cell proliferation, colony formation, and in vivo tumor growth in nude mice xenografts was significantly reduced after the downregulation of MED19. Knockdown of MED19 caused S-phase arrest and induced apoptosis via modulation of Bid and Caspase 7. It was suggested that MED19 serves as a novel proliferation regulator that promotes growth of prostate cancer cells.
authors.affiliation: Urology Department of Surgery, Changzheng Hospital Affiliated to the Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Xingang
authors.initials: X
authors.lastname: Cui
authors.forename: Danfeng
authors.initials: D
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.forename: Fajun
authors.initials: F
authors.lastname: Qu
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Yushan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Jianping
authors.initials: J
authors.lastname: Che
authors.forename: Yacheng
authors.initials: Y
authors.lastname: Yao
authors.forename: Hongyu
authors.initials: H
authors.lastname: Yu
mesh_terms: D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D056892:Mediator Complex / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D034741:RNA, Small Interfering / Q000378:metabolism*; D016196:S Phase; D013489:Suppression, Genetic; D014410:Tumor Stem Cell Assay
chemical_list: C558319:MED19 protein, human; D056892:Mediator Complex; D034741:RNA, Small Interfering