title: DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.
abstract: It was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-β in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-β ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão 121, Salvador, Bahia, Brazil.
authors.forename: Robson A A
authors.initials: RA
authors.lastname: da Silva
authors.forename: Natália M
authors.initials: NM
authors.lastname: Tavares
authors.forename: Dirceu
authors.initials: D
authors.lastname: Costa
authors.forename: Maiana
authors.initials: M
authors.lastname: Pitombo
authors.forename: Larissa
authors.initials: L
authors.lastname: Barbosa
authors.forename: Kyioshi
authors.initials: K
authors.lastname: Fukutani
authors.forename: Jose C
authors.initials: JC
authors.lastname: Miranda
authors.forename: Camila I
authors.initials: CI
authors.lastname: de Oliveira
authors.forename: Jesus G
authors.initials: JG
authors.lastname: Valenzuela
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
authors.forename: Manuel
authors.initials: M
authors.lastname: Soto
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: Cláudia
authors.initials: C
authors.lastname: Brodskyn
mesh_terms: D000818:Animals; D006224:Cricetinae; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000517:prevention & control*; D008099:Liver / Q000469:parasitology; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D008647:Mesocricetus; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D011576:Psychodidae / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000469:parasitology; D016212:Transforming Growth Factor beta / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D019476:Insect Proteins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012471:Salivary Proteins and Peptides; D016212:Transforming Growth Factor beta; D019444:Vaccines, DNA; C090009:kinetoplastid membrane protein 11, Leishmania donovani; D016753:Interleukin-10; D007371:Interferon-gamma