title: Activity of matrix metalloproteinases in bovine versus human dentine.
abstract: Metalloproteinases (MMPs) have been implicated with metabolism of collagen in physiological and pathological processes in human dentine. As bovine teeth have been used as a substitute for human teeth in laboratory analysis, this study evaluated the activity of MMP-2 and -9 in bovine versus human dentine. Bovine and human dentine fragments, from crowns and roots, were powderized. Protein extraction was performed by two protocols: a neutral extraction with guanidine-HCl/EDTA (pH 7.4) and an acidic extraction with citric acid (pH 2.3). Gelatinolytic activities of extracts were revealed by zymography. MMP-2 and -9 were detected in crown and root dentine from bovine and human teeth. Total activities of MMP-2 were 11.4 ± 2.2, 14.6 ± 2.0, 9.7 ± 1.2 and 12.4 ± 0.9 ng/ml for bovine root, human root, bovine crown and human crown dentine, respectively. Corresponding activities for MMP-9 were 14.9 ± 2.0, 15.3 ± 1.3, 15.4 ± 1.3 and 15.5 ± 1.3 ng/ml, respectively. Bovine dentine was found to be a reliable substrate for studies involving the activity of MMP-2 and -9.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, USP-University of São Paulo, Bauru, Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Kato
authors.forename: A R
authors.initials: AR
authors.lastname: Hannas
authors.forename: A L
authors.initials: AL
authors.lastname: Leite
authors.forename: A
authors.initials: A
authors.lastname: Bolanho
authors.forename: B L
authors.initials: BL
authors.lastname: Zarella
authors.forename: J
authors.initials: J
authors.lastname: Santos
authors.forename: M
authors.initials: M
authors.lastname: Carrilho
authors.forename: L
authors.initials: L
authors.lastname: Tjäderhane
authors.forename: M A R
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002417:Cattle; D003804:Dentin / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007180:Incisor / Q000201:enzymology; D020778:Matrix Metalloproteinase 2 / Q000032:analysis / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000378:metabolism*; D008964:Molar, Third / Q000201:enzymology; D011506:Proteins / Q000032:analysis; D019228:Tooth Crown / Q000201:enzymology; D014092:Tooth Root / Q000201:enzymology; D055815:Young Adult
chemical_list: D011506:Proteins; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9