title: Vitamin E modulates cigarette smoke extract-induced cell apoptosis in mouse embryonic cells.
abstract: Vitamin E (VE) can effectively prevent occurrence of lung cancer caused by passive smoking in mice. However, whether VE prevents smoking-induced cytotoxicity remains unclear. In this study, a primary culture of embryonic lung cells (ELCs) was used to observe the cytotoxic effects of cigarette smoke extract (CSE), including its influence on cell survival, cell cycle, apoptosis, and DNA damage, and also to examine the effects of VE intervention on CSE-induced cytotoxicity. Our results showed that CSE could significantly inhibit the survival of ELCs with dose- and time-dependent effects. Furthermore, CSE clearly disturbed the cell cycle of ELCs by decreasing the proportion of cells at the S and G₂/M phases and increasing the proportion of cells at the G₀/G₁ phase. CSE promoted cell apoptosis, with the highest apoptosis rate reaching more than 40%. CSE also significantly caused DNA damage of ELCs. VE supplementation could evidently inhibit or reverse the cytotoxic effects of CSE in a dose- and time-dependent manner. The mechanism of CSE effects on ELCs and that of VE intervention might involve the mitochondrial pathway of cytochrome c-mediated caspase activation. Our study validate that VE plays a clearly protective effect against CSE-induced cytotoxicity in mouse embryonic lung cells.
authors.affiliation: Department of Health and Environment, Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, No.1, Dali Road, Tianjin 300050, P. R. China.
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Tao
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Yuan
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Shen
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Li
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Qiu
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Xin-Wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D008168:Lung / Q000166:cytology / Q000196:embryology; D051379:Mice; D012907:Smoking / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects / Q000737:chemistry*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D014810:Vitamin E