title: Persistent inflammation leads to proliferative neoplasia and loss of smooth muscle cells in a prostate tumor model.
abstract: In prostate cancers, epidemiological data suggest a link between prostate inflammation and subsequent cancer development, but proof for this concept in a tumor model is lacking. A constitutively active version of IκB kinase 2 (IKK2), which is activated by many inflammatory stimuli, was expressed specifically in the prostate epithelium. Constitutive activation of the IKK2/nuclear factor κB axis was insufficient for prostate transformation. However, in combination with heterozygous loss of phosphatase and tensin homolog, IKK2 activation led to an increase in tumor size, formation of cribriform structures, and increase in fiber in the fibroblastic stroma. This phenotype was coupled with persistent inflammation evoked by chemokine expression in the epithelium and stroma. The hyperplastic and dysplastic epithelia correlated with changes evoked by decreased androgen receptor activation. Conversely, inflammation correlated with stromal changes highlighted by loss of smooth muscle cells around prostate ducts. Despite the loss of the smooth muscle barrier, tumors were rarely invasive in a C57BL/6 background. Data mining revealed that smooth muscle markers are also downregulated in human prostate cancers, and loss of these markers in primary tumors is associated with subsequent metastasis. In conclusion, our data show that loss of smooth muscle and invasiveness of the tumor are not coupled in our model, with inflammation leading to increased tumor size and a dedifferentiated stroma.
authors.affiliation: Department of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, Austria. andreas.birbach@meduniwien.ac.at
authors.forename: Andreas
authors.initials: A
authors.lastname: Birbach
authors.forename: David
authors.initials: D
authors.lastname: Eisenbarth
authors.forename: Nicolas
authors.initials: N
authors.lastname: Kozakowski
authors.forename: Eva
authors.initials: E
authors.lastname: Ladenhauf
authors.forename: Marc
authors.initials: M
authors.lastname: Schmidt-Supprian
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Schmid
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D018925:Chemokines / Q000096:biosynthesis; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D051550:I-kappa B Kinase / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D011467:Prostate / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011472:Prostatitis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D047368:Tumor Burden / Q000235:genetics
chemical_list: D018925:Chemokines; D011944:Receptors, Androgen; D051550:I-kappa B Kinase; D051059:PTEN Phosphohydrolase; C487757:Pten protein, mouse