title: Smart DNA vectors based on cyclodextrin polymers: compaction and endosomal release.
abstract: OBJECTIVE
  Neutral β-cyclodextrin polymers (polyβCD) associated with cationic adamantyl derivatives (Ada) can be used to deliver plasmid DNA into cells. In absence of an endosomolytic agent, transfection efficiency remains low because most complexes are trapped in the endosomal compartment. We asked whether addition of an imidazole-modified Ada can increase efficiency of polyβCD/cationic Ada-based delivery system.
  
  
  METHODS
  We synthesized two adamantyl derivatives: Ada5, which has a spacer arm between the Ada moiety and a bi-cationic polar head group, and Ada6, which presents an imidazole group. Strength of association between polyβCD and Ada derivatives was evaluated by fluorimetric titration.
  
  
  RESULTS
  Gel mobility shift assay, zeta potential, and dark field transmission electron microscopy experiments demonstrated the system allowed for efficient DNA compaction. In vitro transfection experiments performed on HepG2 and HEK293 cells revealed the quaternary system polyβCD/Ada5/Ada6/DNA has efficiency comparable to cationic lipid DOTAP.
  
  
  CONCLUSIONS
  We successfully designed fine-tuned DNA vectors based on cyclodextrin polymers combined with two new adamantyl derivatives, leading to significant transfection associated with low toxicity.
authors.affiliation: Institut de Chimie et des Matériaux Paris Est, ICMPE Systèmes Polymères Complexes, UMR 7182, 2-8 rue Henri Dunant, 94320, Thiais, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Wintgens
authors.forename: Christian
authors.initials: C
authors.lastname: Leborgne
authors.forename: Sonia
authors.initials: S
authors.lastname: Baconnais
authors.forename: Virginie
authors.initials: V
authors.lastname: Burckbuchler
authors.forename: Eric
authors.initials: E
authors.lastname: Le Cam
authors.forename: Daniel
authors.initials: D
authors.lastname: Scherman
authors.forename: Antoine
authors.initials: A
authors.lastname: Kichler
authors.forename: Catherine
authors.initials: C
authors.lastname: Amiel
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives*; D002482:Cellulose / Q000737:chemistry*; D003505:Cyclodextrins / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection
chemical_list: D003505:Cyclodextrins; D007093:Imidazoles; C041953:cyclodextrin polymer; C029899:imidazole; D002482:Cellulose; D004247:DNA; D000218:Adamantane