title: Degradation of transgene DNA in genetically modified herbicide-tolerant rice during food processing.
abstract: In order to assess the effect of food processing on the degradation of exogenous DNA components in sweet rice wine and rice crackers made from genetically modified (GM) rice (Oryza sativa L.), we developed genomic DNA extraction methods and compared the effect of different food processing procedures on DNA degradation. It was found that the purity, quantity and quality of DNA by alkaline lysis method were higher than by CTAB (cetyltrimethylammonium bromide) method. For sweet rice wine, CAMV35S (cauliflower mosaic virus 35S) promoter and NOS (nopaline synthase) terminator were degraded by the third day, whereas the exogenous gene Bar (bialaphos resistance) remained unaffected. For rice crackers, boiling, drying and microwaving contributed to the initial degradations of DNA. Baking resulted in further degradations, and frying led to the most severe changes. These results indicated that the stability of DNA in GM rice was different under different processing conditions. For sweet rice wine, Bar was most stable, followed by NOS, CAMV35S, and SPS. For rice crackers, CAMV35S was most stable, followed by SPS, NOS, and Bar.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shangxin
authors.initials: S
authors.lastname: Song
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Liangyan
authors.initials: L
authors.lastname: Qiu
authors.forename: Sifa
authors.initials: S
authors.lastname: Dai
authors.forename: Xinglian
authors.initials: X
authors.lastname: Xu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics; D017796:Caulimovirus / Q000235:genetics; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000378:metabolism; D018744:DNA, Plant / Q000737:chemistry*; D005511:Food Handling / Q000379:methods*; D006540:Herbicides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism; D012275:Oryza / Q000737:chemistry / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics; D011401:Promoter Regions, Genetic; D019076:Transgenes
chemical_list: D002593:Cetrimonium Compounds; D018744:DNA, Plant; D006540:Herbicides; D009943:Organophosphorus Compounds; C003122:bialaphos; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase; D000077286:Cetrimonium