title: The plant cell death suppressor Adi3 interacts with the autophagic protein Atg8h.
abstract: The tomato AGC protein kinase Adi3 is known to function as a suppressor of PCD and silencing of Adi3 leads to spontaneous cell death on leaves and stems. In an effort to isolate Adi3 interacting proteins, a yeast two-hybrid screen was carried out and identified the autophagy protein Atg8h as an Adi3 interactor. This interaction occurred independent of the kinase activity status of Adi3. Silencing of genes involved in autophagy is known to eliminate the restriction of pathogen-induced PCD to a few cells and leads to run away PCD. Cosilencing Adi3 with several autophagy genes lead to the same run away cell death suggesting Adi3 may be involved in autophagic regulation of PCD.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128, USA. tpd8@tamu.edu
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Devarenne
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis; D017360:Arabidopsis / Q000201:enzymology / Q000378:metabolism; D001343:Autophagy; D020868:Gene Silencing; D018551:Solanum lycopersicum / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D059828:Plant Cells; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D011198:Solanum tuberosum; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D011494:Protein Kinases