title: Bioinformatics and genomic analysis of transposable elements in eukaryotic genomes.
abstract: A major portion of most eukaryotic genomes are transposable elements (TEs). During evolution, TEs have introduced profound changes to genome size, structure, and function. As integral parts of genomes, the dynamic presence of TEs will continue to be a major force in reshaping genomes. Early computational analyses of TEs in genome sequences focused on filtering out "junk" sequences to facilitate gene annotation. When the high abundance and diversity of TEs in eukaryotic genomes were recognized, these early efforts transformed into the systematic genome-wide categorization and classification of TEs. The availability of genomic sequence data reversed the classical genetic approaches to discovering new TE families and superfamilies. Curated TE databases and their accurate annotation of genome sequences in turn facilitated the studies on TEs in a number of frontiers including: (1) TE-mediated changes of genome size and structure, (2) the influence of TEs on genome and gene functions, (3) TE regulation by host, (4) the evolution of TEs and their population dynamics, and (5) genomic scale studies of TE activity. Bioinformatics and genomic approaches have become an integral part of large-scale studies on TEs to extract information with pure in silico analyses or to assist wet lab experimental studies. The current revolution in genome sequencing technology facilitates further progress in the existing frontiers of research and emergence of new initiatives. The rapid generation of large-sequence datasets at record low costs on a routine basis is challenging the computing industry on storage capacity and manipulation speed and the bioinformatics community for improvement in algorithms and their implementations.
authors.affiliation: Department of Biology, University of Toronto at Mississauga, 3359 Mississauga Road, Mississauga, ON L5L1C6, Canada.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Janicki
authors.forename: Rebecca
authors.initials: R
authors.lastname: Rooke
authors.forename: Guojun
authors.initials: G
authors.lastname: Yang
mesh_terms: D019295:Computational Biology; D004251:DNA Transposable Elements; D030541:Databases, Genetic; D056890:Eukaryota / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D059646:Genome Size; D023281:Genomics; D058977:Molecular Sequence Annotation; D017422:Sequence Analysis, DNA; D012984:Software
chemical_list: D004251:DNA Transposable Elements