title: Molecular characterization of Theileria orientalis causing fatal infection in crossbred adult bovines of South India.
abstract: The disease condition attributed to have been caused by Theileria orientalis is generally benign. However, it is also thought that the parasite, at least some strains of it, can cause fatal disease. The present communication deals with the clinical signs, postmortem lesions and diagnosis of a fatal disease due to T. orientalis which caused mortality in crossbred adult bovines of South India. High body temperature, lacrimation, nasal discharge, swollen lymph nodes and haemoglobinuria were the symptoms observed. The postmortem lesions observed were punched out ulcers in abomasum, enlargement of spleen, massive pulmonary oedema, frothy exudates in trachea, epicardial and endocardial haemorrhage and haemorrhagic duodenitis. Peripheral blood smear examination revealed rod shaped Theileria sp. organisms. Polymerase chain reaction that amplify the T. orientalis specific P(32/33) gene, followed by cloning and sequencing, revealed maximum homology with Narathiwat (Thailand) and Jingole -1 (Indonesia) isolates which were positioned as isolate type 7 of T. orientalis.
authors.affiliation: College of Veterinary and Animal Sciences, Pookode, Wayanad, Kerala, India.
authors.forename: M
authors.initials: M
authors.lastname: Aparna
authors.forename: Reghu
authors.initials: R
authors.lastname: Ravindran
authors.forename: M B
authors.initials: MB
authors.lastname: Vimalkumar
authors.forename: Bindu
authors.initials: B
authors.lastname: Lakshmanan
authors.forename: P
authors.initials: P
authors.lastname: Rameshkumar
authors.forename: K G Ajith
authors.initials: KG
authors.lastname: Kumar
authors.forename: K
authors.initials: K
authors.lastname: Promod
authors.forename: S
authors.initials: S
authors.lastname: Ajithkumar
authors.forename: Chintu
authors.initials: C
authors.lastname: Ravishankar
authors.forename: K
authors.initials: K
authors.lastname: Devada
authors.forename: H
authors.initials: H
authors.lastname: Subramanian
authors.forename: Ajith Jacob
authors.initials: AJ
authors.lastname: George
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000401:mortality / Q000469:parasitology / Q000635:transmission; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics*; D017125:Genes, Protozoan; D007194:India / Q000453:epidemiology; D007214:Indonesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D015996:Survival Rate; D013785:Thailand; D016794:Theileria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D013801:Theileriasis / Q000097:blood / Q000175:diagnosis* / Q000401:mortality / Q000469:parasitology / Q000635:transmission
chemical_list: D016054:DNA, Protozoan