title: Do serum angiopoietin-1, angiopoietin-2, and their receptor Tie-2 and 4G/5G variant of PAI-1 gene have a role in the pathogenesis of preeclampsia?
abstract: OBJECTIVE
  To evaluate whether serum angiogenesis markers such as angiopoietins (Ang-1, Ang-2) and their receptor (Tie-2) are altered in women with preeclampsia. We also performed genotyping to determine if the 4G/5G genotypes of -675 PAI-1 gene may play a role in the pathogenesis of preeclampsia.
  
  
  METHODS
  Sixty-eight pregnant women with preeclampsia were compared to 35 normotensive pregnant women and 24 normotensive nonpregnant women in a cross-sectional study. Using enzyme-linked immunosorbent assay, levels of serum Ang-1 and Ang-2, and Tie-2 were measured. A single base pair insertion/deletion 4G/5G polymorphism of the PAI-1 gene was determined by polymerase chain reaction.
  
  
  RESULTS
  Serum levels of Ang-1 and Tie-2 were significantly different among the study groups (P = 0.001 and P = 0.025, respectively) being lower in the preeclamptic group. Positive significant correlation was found between Ang-2 and Tie-2, (r = 0.26, P = 0.024). The frequency of the genotypes (4G/5G, 4G/4G, and 5G/5G) differed among the groups (P = 0.001). Also, the mean of systolic and diastolic blood pressures differed significantly according to the PAI-1 genotype being higher in those bearing the 4G allele; P = 0.04 and P = 0.023, respectively.
  
  
  CONCLUSIONS
  Sera Ang-1 and Ang-2, and Tie-2 as well as variants of 4G/5G of PAI-1 polymorphism have positive implications in the pathogenesis of preeclampsia.
authors.affiliation: Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt. manalmk@yahoo.com
authors.forename: Manal
authors.initials: M
authors.lastname: Kamal
authors.forename: Waleed
authors.initials: W
authors.lastname: El-Khayat
mesh_terms: D000328:Adult; D000483:Alleles; D042683:Angiopoietin-1 / Q000097:blood*; D042702:Angiopoietin-2 / Q000097:blood*; D015415:Biomarkers / Q000378:metabolism; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D009389:Neovascularization, Pathologic / Q000473:pathology; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood* / Q000235:genetics; D011225:Pre-Eclampsia / Q000097:blood* / Q000235:genetics; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics; D011507:Proteinuria / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000097:blood*
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D015415:Biomarkers; D017395:Plasminogen Activator Inhibitor 1; D042787:Receptor, TIE-2