title: Antiglycation and antioxidant properties of soy sauces.
abstract: Diabetes-induced hyperglycemia increases formation of advanced glycation end products (AGEs) and metal-catalyzed production of free radicals. This study compared the antioxidant capacities of dark and light soy sauces of different brands and investigated their abilities to inhibit AGEs and whether their mechanism of action was pre- or post-Amadori or involved chelation of transition metals. The antioxidant capacities of soy sauces were compared using the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) method and by measuring their total phenolic contents. Model proteins (lysozyme, albumin) were glycated using fructose with or without soy sauces with subsequent analysis of cross-linked AGEs by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The effect of soy sauces on pre- and post-Amadori inhibition of AGEs was investigated by measuring fructosamine and AGEs following reincubation of ribose-glycated (ribated) lysozyme, respectively. Dark soy sauces had higher antioxidant capacities and phenolic content and were more effective inhibitors of post-Amadori-derived cross-linked AGEs. However, light soy sauces were more effective at inhibiting fructosamine and had more potent metal chelation properties. This study reports the antiglycation properties of soy sauces, but further studies are required to determine the constituents responsible for this effect and whether soy sauce consumption can reduce oxidative stress and AGEs in diabetic subjects.
authors.affiliation: School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom.
authors.forename: Changwe
authors.initials: C
authors.lastname: Mashilipa
authors.forename: Qiuyu
authors.initials: Q
authors.lastname: Wang
authors.forename: Mark
authors.initials: M
authors.lastname: Slevin
authors.forename: Nessar
authors.initials: N
authors.lastname: Ahmed
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D052160:Benzothiazoles / Q000378:metabolism; D019270:Fructosamine / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D019800:Phenol / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D045730:Soy Foods / Q000032:analysis*; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D017127:Glycation End Products, Advanced; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D019800:Phenol; D019270:Fructosamine