title: Role and regulation of autophagy in the development of acinar structures formed by bovine BME-UV1 mammary epithelial cells.
abstract: Autophagy is a catabolic process providing an alternative energy source for cells under stressful conditions such as starvation, growth factor deprivation or hypoxia. During involution of the bovine mammary gland autophagy is induced in mammary epithelial cells (MECs) as a survival mechanism, and is tightly regulated by hormones and growth factors necessary for gland development. In the present study we adapted the three-dimensional culture model to investigate the role of autophagy during formation of alveoli-like structures by bovine BME-UV1 MECs grown on extracellular matrix (ECM) components. Using confocal microscopy and Western-blot analyses of autophagic and apoptotic markers: LC3, and cleaved caspase-3, we showed that autophagy was induced in centrally localized cells within the developing acini. These cells lacked a direct contact with ECM, and formed a distinct population from the outer layer of cells. Induction of autophagy preceded apoptosis, but did not inhibit the formation of a hollow lumen. In the presence of steroid hormones: 17β-estradiol and progesterone, although autophagy was augmented, acini formation proceeded normally. In contrast, the major lactogenic hormone: prolactin, which supports functional differentiation of alveoli, did not alter induction of autophagy within the spheroids. BME-UV1 cells cultured on Matrigel in the presence of growth factors IGF-I and EGF formed larger, underdeveloped acini without lumens due to caspase-3 inhibition, and sustained autophagy in the centre of the spheroids, while TGF-β1 accelerated apoptosis, and increased autophagy significantly. Our observations suggest that sex steroids 17β-estradiol and progesterone, as well as growth factor TGF-β1 may regulate the development of the bovine mammary gland by inducing autophagy in addition to regulating proliferation and apoptosis of MECs. These data indicate that autophagy may play an important role during alveolargenesis.
authors.affiliation: Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sobolewska
authors.forename: Tomasz
authors.initials: T
authors.lastname: Motyl
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Gajewska
mesh_terms: D000818:Animals; D017209:Apoptosis; D001343:Autophagy; D053148:Caspase 3 / Q000378:metabolism; D002417:Cattle; D002449:Cell Aggregation; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology / Q000502:physiology; D005260:Female; D006133:Growth Substances / Q000494:pharmacology / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000502:physiology; D007797:Laminin / Q000737:chemistry; D008321:Mammary Glands, Animal / Q000166:cytology*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011374:Progesterone / Q000494:pharmacology / Q000502:physiology; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology; D011509:Proteoglycans / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology / Q000502:physiology
chemical_list: D003470:Culture Media; D004338:Drug Combinations; D006133:Growth Substances; D007797:Laminin; D011509:Proteoglycans; D053773:Transforming Growth Factor beta1; C059485:matrigel; D011374:Progesterone; D004958:Estradiol; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I; D011388:Prolactin; D003094:Collagen; D053148:Caspase 3