title: Fez function is required to maintain the size of the animal plate in the sea urchin embryo.
abstract: Partitioning ectoderm precisely into neurogenic and non-neurogenic regions is an essential step for neurogenesis of almost all bilaterian embryos. Although it is widely accepted that antagonism between BMP and its inhibitors primarily sets up the border between these two types of ectoderm, it is unclear how such extracellular, diffusible molecules create a sharp and precise border at the single-cell level. Here, we show that Fez, a zinc finger protein, functions as an intracellular factor attenuating BMP signaling specifically within the neurogenic region at the anterior end of sea urchin embryos, termed the animal plate. When Fez function is blocked, the size of this neurogenic ectoderm becomes smaller than normal. However, this reduction is rescued in Fez morphants simply by blocking BMP2/4 translation, indicating that Fez maintains the size of the animal plate by attenuating BMP2/4 function. Consistent with this, the gradient of BMP activity along the aboral side of the animal plate, as measured by pSmad1/5/8 levels, drops significantly in cells expressing Fez and this steep decline requires Fez function. Our data reveal that this neurogenic ectoderm produces an intrinsic system that attenuates BMP signaling to ensure the establishment of a stable, well-defined neural territory, the animal plate.
authors.affiliation: Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan. yag@kurofune.shimoda.tsukuba.ac.jp
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Yaguchi
authors.forename: Junko
authors.initials: J
authors.lastname: Yaguchi
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wei
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Jin
authors.forename: Lynne M
authors.initials: LM
authors.lastname: Angerer
authors.forename: Kazuo
authors.initials: K
authors.lastname: Inaba
mesh_terms: D000818:Animals; D036703:Blastula / Q000378:metabolism; D019521:Body Patterning / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000737:chemistry*; D019070:Cell Lineage; D004475:Ectoderm / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry / Q000379:methods; D008954:Models, Biological; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012617:Sea Urchins; D051785:Smad Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D016335:Zinc Fingers
chemical_list: D019485:Bone Morphogenetic Proteins; D051785:Smad Proteins; D014157:Transcription Factors