title: Dendritic cells transfected with PD-L1 recombinant adenovirus induces T cell suppression and long-term acceptance of allograft transplantation.
abstract: The purpose of this study is to assess the potential of dendritic cells transfected with PD-L1 recombinant adenovirus induces CD8+ T cell suppression and kidney allograft tolerance. To prove it, DCs transfected with PD-L1 recombinant adenovirus (DC/Ad-PD-L1) were transferred into the MHC-mismatched rat kidney transplants. After kidney transplantation, the mixed lymphocyte reaction (MLR) assay and kidney function were analyzed. The results demonstrated that after administration of DC/Ad-PD-L1, the proliferation, cytokines secretion and activation marker expression of CD8+ T cells were suppressed. In addition, DC/Ad-PD-L1 could improve kidney function and survival of transplants. The findings suggested that DC/Ad-PD-L1 could induce CD8+ T cell tolerance and lead to kidney allograft tolerance, which provided a promising finding for clinical application.
authors.affiliation: Department of Orthopaedics, 309th Hospital of PLA, Beijing 100091, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Boli
authors.initials: B
authors.lastname: Ran
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Ma
authors.forename: Xunwu
authors.initials: X
authors.lastname: Huang
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D060890:B7-H1 Antigen / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism; D016030:Kidney Transplantation / Q000379:methods; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D011507:Proteinuria / Q000276:immunology / Q000652:urine; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D023001:Transplantation Tolerance / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D060890:B7-H1 Antigen; D007376:Interleukin-2; D011993:Recombinant Fusion Proteins; D007371:Interferon-gamma