title: Evaluation study of a portable impedance biosensor for detection of avian influenza virus.
abstract: Current methods for detection of avian influenza virus (AIV) based on virus culture and RT-PCR are well established, but they are either time consuming or require specialized laboratory facilities and highly trained technicians. A simple, rapid, robust, and reliable test, suitable for use in the field or at the patient's bedside, is urgently needed. In this study, the performance of a newly developed portable impedance biosensor was evaluated by comparison with real-time reverse transcriptase PCR (rRT-PCR) and virus culture for detection of AIV in tracheal and cloacal swab samples collected from experimentally H5N2 AIV infected chickens. The impedance biosensor system was based on a combination of magnetic nanobeads, which were coated with AIV subtype-specific antibody for capture (separation and concentration) of a target virus, and a microfluidic chip with an interdigitated array microelectrode for transfer and detection of target virus, and impedance measurement of the bio-nanobeads and AI virus complexes in a buffer solution. A comparison of results obtained from 59 swab samples using virus culture, impedance biosensor and rRT-PCR methods showed that the impedance biosensor technique was comparable in sensitivity and specificity to rRT-PCR. Detection time for the impedance biosensor is less than 1h.
authors.affiliation: Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Wang
authors.forename: Jianhan
authors.initials: J
authors.lastname: Lin
authors.forename: Kentu
authors.initials: K
authors.lastname: Lassiter
authors.forename: Balaji
authors.initials: B
authors.lastname: Srinivasan
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Huaguang
authors.initials: H
authors.lastname: Lu
authors.forename: Steve
authors.initials: S
authors.lastname: Tung
authors.forename: Billy
authors.initials: B
authors.lastname: Hargis
authors.forename: Walter
authors.initials: W
authors.lastname: Bottje
authors.forename: Luc
authors.initials: L
authors.lastname: Berghman
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D015374:Biosensing Techniques / Q000379:methods*; D002645:Chickens; D002988:Cloaca / Q000821:virology; D017097:Electric Impedance; D053125:Influenza A Virus, H5N2 Subtype / Q000302:isolation & purification*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D058185:Magnetite Nanoparticles; D044085:Microfluidics; D019095:Point-of-Care Systems; D012680:Sensitivity and Specificity; D014132:Trachea / Q000821:virology; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D058185:Magnetite Nanoparticles