title: Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers.
abstract: Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Grosso State, Brazil. Internal transcribed spacer-RFLP did not differentiate the isolates of M. anisopliae var. anisopliae, while ISSR and intron primers identified three distinct groups. Variability among these groups was 96% for (GTG)(5) and 100% for the other primers. We found considerable genetic variability, even among isolates from the same geographical origin and host.
authors.affiliation: Departamento de Micologia, Universidade Federal de Pernambuco, Recife, PE, Brasil. patiago@hotmail.com
authors.forename: P V
authors.initials: PV
authors.lastname: Tiago
authors.forename: M P
authors.initials: MP
authors.lastname: Carneiro-Leão
authors.forename: M L A
authors.initials: ML
authors.lastname: Lima
authors.forename: N T
authors.initials: NT
authors.lastname: Oliveira
authors.forename: E A
authors.initials: EA
authors.lastname: Luna-Alves Lima
mesh_terms: D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D014644:Genetic Variation; D006999:Hypocreales / Q000235:genetics*; D007438:Introns; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D022821:RNA Splice Sites
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D022821:RNA Splice Sites