title: Prediction of oocyte developmental competence in ovine using glucose-6-phosphate dehydrogenase (G6PDH) activity determined at retrieval time.
abstract: OBJECTIVE
  To determine whether G6PDH-activity measured by Brilliant Cresyl Blue known as BCB dye, predicts developmental competence within cohorts of ovine oocytes.
  
  
  METHODS
  Ovine oocytes were exposed to BCB staining and categorized into two groups: BCB+ (blue cytoplasm, low G6PDH-activity) and BCB- (colorless cytoplasm, high G6PDH-activity). After maturation in vitro, oocytes were subjected to fertilization followed by in vitro embryo culture.
  
  
  RESULTS
  We observed a significant difference in oocyte diameter considering BCB+ and BCB- oocytes. BCB+ and Control groups showed significantly higher maturation rates compared to BCB- group. There were significantly more cleaved embryos in BCB+ and control groups than in BCB- group. Blastocyst rate was significantly higher for BCB+ group compared to control and BCB- groups with control group being significantly higher than BCB- group.
  
  
  CONCLUSIONS
  G6PDH-activity is a strong predictive marker of oocyte competence and may be useful in identifying oocytes with a good prognosis for further develop.
authors.affiliation: Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran. a.mohammadis@stemcellstech.com
authors.forename: Abdollah
authors.initials: A
authors.lastname: Mohammadi-Sangcheshmeh
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Hamid
authors.initials: H
authors.lastname: Deldar
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.forename: Sara
authors.initials: S
authors.lastname: Soudi
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Hashemi
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.forename: Michael
authors.initials: M
authors.lastname: Hoelker
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D059471:In Vitro Oocyte Maturation Techniques; D009865:Oocytes / Q000201:enzymology* / Q000254:growth & development*; D011237:Predictive Value of Tests; D012756:Sheep / Q000196:embryology
chemical_list: D005954:Glucosephosphate Dehydrogenase