title: Effects of in utero exposure to D-004, a lipid extract from Roystonea regia fruits, in the male rat: a comparison with finasteride.
abstract: D-004 is a lipid extract obtained from Cuban royal palm fruits, consisting of a mixture of free fatty acids, that prevents prostate hyperplasia induced with testosterone in rodents. This study investigated the possible alterations due to D-004 of androgen-dependent development after exposure in utero and compared them with those due to finasteride. Rats were randomized into five experimental groups: a control group, three groups treated with D-004 at 500, 750, or 1,000 mg/kg/day, respectively, and a group treated with finasteride (10 mg/kg/day). Male rats were treated 10 weeks before and during mating. Female rats were treated for 15 days prior mating, during mating, during pregnancy, and until lactation (day 21) except for those treated with finasteride, which were only administered the drug on gestational days 12-21. All male offspring were monitored individually until necropsy after postnatal day 90. The results of the present study indicate that D-004 induced no alterations in androgen-dependent development after the exposure in utero. Also, the current study demonstrated a permanent reduction in anogenital distance and retention of nipples in adult male rats exposed to finasteride during late gestation. Significant alterations induced by exposure to finasteride were mainly in tissues dependent on dihydrotestosterone during development.
authors.affiliation: Center of Natural Products, National Center for Scientific Research, Cubanacan, Havana, Cuba. ariadne.gutierrez@cnic.edu.cu
authors.forename: Ariadne Gutiérrez
authors.initials: AG
authors.lastname: Martínez
authors.forename: Balia
authors.initials: B
authors.lastname: Pardo
authors.forename: Rafael
authors.initials: R
authors.lastname: Gámez
authors.forename: Rosa
authors.initials: R
authors.lastname: Mas
authors.forename: Miriam
authors.initials: M
authors.lastname: Noa
authors.forename: Gisela
authors.initials: G
authors.lastname: Marrero
authors.forename: Maikel
authors.initials: M
authors.lastname: Valle
authors.forename: Haydee
authors.initials: H
authors.lastname: García
authors.forename: Dayisell
authors.initials: D
authors.lastname: Curveco
authors.forename: Nilda
authors.initials: N
authors.lastname: Mendoza
authors.forename: Edy
authors.initials: E
authors.lastname: Goicochea
mesh_terms: D000728:Androgens / Q000378:metabolism; D000818:Animals; D028023:Arecaceae / Q000737:chemistry*; D005260:Female; D018120:Finasteride / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D008055:Lipids / Q000302:isolation & purification*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011470:Prostatic Hyperplasia / Q000139:chemically induced / Q000517:prevention & control; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000009:adverse effects
chemical_list: D000728:Androgens; C504503:D 004 extract; D008055:Lipids; D010936:Plant Extracts; D013739:Testosterone; D018120:Finasteride