title: A biological breadboard platform for cell adhesion and detachment studies.
abstract: The dynamic nature of cell adhesion and detachment, which plays a critical role in a variety of physiological and pathological phenomena, still remains unclear. This motivates the pursuit of controllable manipulation of cell adhesion and detachment for a better understanding of cellular dynamics. Here we present an addressable, multifunctional, and reusable platform, termed the biological breadboard (BBB), for spatiotemporal manipulation of cell adhesion and detachment at cellular and subcellular levels. The BBB, composed of multiple gold electrodes patterned on a Pyrex substrate, is surface-modified with arginine-glycine-aspartic acid terminated thiol (RTT) and polyethylene glycol (PEG) to achieve a cell-adhesive surface on the gold electrodes and a cell-resistive surface on the Pyrex substrate, respectively. Cell adhesion is regulated by the steric repulsion of PEG chains, while cell detachment is controlled by the reductive desorption of a gold-thiol self-assembled monolayer (SAM) at an activation potential of -0.90 to -1.65 V. Experimental characterizations using NIH 3T3 fibroblasts are presented to demonstrate the utility of our device.
authors.affiliation: Molecular Cell Biomechanics Laboratory, Department of Bioengineering, University of California, Berkeley, California 94720, USA.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Yoon
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Chang
authors.forename: Liwei
authors.initials: L
authors.lastname: Lin
authors.forename: Mohammad R K
authors.initials: MR
authors.lastname: Mofrad
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002470:Cell Survival; D003584:Cytological Techniques / Q000295:instrumentation*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D055616:Microtechnology / Q000295:instrumentation*; D041681:NIH 3T3 Cells; D055620:Optical Phenomena; D011092:Polyethylene Glycols / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013997:Time Factors
chemical_list: D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold