title: Effect of pH on protein adsorption capacity of strong cation exchangers with grafted layer.
abstract: The effect of pH on the static adsorption capacity of immunoglobulin G, human serum albumin, and equine myoglobin was investigated for a set of five strong cation exchangers with the grafted tentacle layer having a different ligand density. A sharp maximum of adsorption capacity with pH was observed for adsorbents with a high ligand density. The results were elucidated using the protein structure and calculations of pK(a) of ionizable groups of surface basic residues. Inverse size-exclusion experiments were carried out to understand the relation between the adsorption capacity and pore accessibility of the investigated proteins.
authors.affiliation: Slovak University of Technology, Faculty of Chemical and Food Technology, Institute of Chemical and Environmental Engineering, Department of Chemical and Biochemical Engineering, Radlinského 9, 812 37 Bratislava, Slovakia.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wrzosek
authors.forename: Milan
authors.initials: M
authors.lastname: Polakovič
mesh_terms: D000327:Adsorption; D000818:Animals; D002411:Cation Exchange Resins / Q000737:chemistry*; D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods*; D006736:Horses; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D009211:Myoglobin / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012709:Serum Albumin / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D002411:Cation Exchange Resins; D007074:Immunoglobulin G; D009211:Myoglobin; D011506:Proteins; D012709:Serum Albumin; D012965:Sodium Chloride