title: Controlling the dimensions of amyloid fibrils: toward homogenous components for bionanotechnology.
abstract: Amyloid fibrils have been recognized as having potential in a variety of bionanotechnological applications. However, realization of these applications is constrained by a lack of control over morphology and alignment, both crucial for potential end uses. This article focuses on the use of growth and storage conditions to control the length of amyloid fibrils formed from bovine insulin, with length distributions constructed from transmission electron microscopy (TEM) images. Growth temperature, pH, protein concentration, and storage conditions were examined and were seen to offer a range of conditions that favor different length distribution. The use of amyloid fibrils as nanowires is one area where control of fibril dimensions is desirable, for experimental setup and endpoint applications. The conductive properties of fibrils formed from bovine insulin are presented, with these insulin fibrils being shown to have high resistivity in their unmodified state, with current values in the nanoamp range. These low current values can be increased via modification, or the fibrils used in their native state in applications where low current values are desirable. These findings, coupled with the ability to predict and select for various insulin amyloid fibril dimensions, enhances their utility as nanomaterials.
authors.affiliation: School of Biological Sciences, University of Canterbury, New Zealand. laura.domigan@pg.canterbury.ac.nz
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Domigan
authors.forename: Jackie P
authors.initials: JP
authors.lastname: Healy
authors.forename: Susie J
authors.initials: SJ
authors.lastname: Meade
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Blaikie
authors.forename: Juliet A
authors.initials: JA
authors.lastname: Gerrard
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D002417:Cattle; D007004:Hypoglycemic Agents / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D011487:Protein Conformation
chemical_list: D000682:Amyloid; D001704:Biopolymers; D007004:Hypoglycemic Agents; D007328:Insulin