title: Modulation of cytokine mRNA expression in pharyngeal epithelial samples obtained from cattle infected with foot-and-mouth disease virus.
abstract: A novel technique of endoscopical collection of small tissue samples was used to obtain sequential tissue samples from the dorsal soft palate (DSP) of individual cattle infected with foot-and-mouth disease virus (FMDV) at different phases of the infection. Levels of mRNA encoding interferon (IFN)-α and IFN-β as well as tumour necrosis factor (TNF)-α were measured in these samples by quantitative reverse transcriptase polymerase chain reaction. Expression of IFN-β mRNA was significantly down-regulated in the biopsy samples harvested during the acute phase of infection, while there was no statistically significant effect on the expression of IFN-α mRNA compared with baseline levels. In contrast, the mRNA encoding TNF-α was significantly up-regulated in samples collected during both acute and late (>28 days post infection) phases of infection. There were also significantly higher levels of TNF-α mRNA expressed in samples derived from animals that were identified subsequently as persistently infected FMDV-carriers. It was concluded that there was a significant difference in the host-response in the DSP of calves that were identified as persistently infected, subclinical carriers of FMDV.
authors.affiliation: National Veterinary Institute, Technical University of Denmark (DTU-Vet), Division of Virology, Lindholm, DK-4771 Kalvehave, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Stenfeldt
authors.forename: P M H
authors.initials: PM
authors.lastname: Heegaard
authors.forename: A
authors.initials: A
authors.lastname: Stockmarr
authors.forename: G J
authors.initials: GJ
authors.lastname: Belsham
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D005536:Foot-and-Mouth Disease / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000378:metabolism*; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism*; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D010614:Pharynx / Q000378:metabolism* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D016898:Interferon-alpha; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta