title: Culture medium, gas atmosphere and MAPK inhibition affect regulation of RNA-binding protein targets during mouse preimplantation development.
abstract: During oogenesis, mammalian oocytes accumulate maternal mRNAs that support the embryo until embryonic genome activation. RNA-binding proteins (RBP) may regulate the stability and turnover of maternal and embryonic mRNAs. We hypothesised that varying embryo culture conditions, such as culture medium, oxygen tension and MAPK inhibition, affects regulation of RBPs and their targets during preimplantation development. STAU1, ELAVL1, KHSRP and ZFP36 proteins and mRNAs were detected throughout mouse preimplantation development, whereas Elavl2 mRNA decreased after the two-cell stage. Potential target mRNAs of RBP regulation, Gclc, Slc2a1 and Slc7a1 were detected during mouse preimplantation development. Gclc mRNA was significantly elevated in embryos cultured in Whitten's medium compared with embryos cultured in KSOMaa, and Gclc mRNA was elevated under high-oxygen conditions. Inhibition of the p38 MAPK pathway reduced Slc7a1 mRNA expression while inhibition of ERK increased Slc2a1 mRNA expression. The half-lives of the potential RBP mRNA targets are not regulated in parallel; Slc2a1 mRNA displayed the longest half-life. Our results indicate that mRNAs and proteins encoding five RBPs are present during preimplantation development and more importantly, demonstrate that expression of RBP target mRNAs are regulated by culture medium, gas atmosphere and MAPK pathways.
authors.affiliation: Department of Physiology, The University of Western Ontario, London, Ontario, Canada A5-146. michele.calder@schulich.uwo.ca
authors.forename: Michele D
authors.initials: MD
authors.lastname: Calder
authors.forename: Patricia H
authors.initials: PH
authors.lastname: Watson
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Watson
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D005260:Female; D005740:Gases / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D051379:Mice; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005740:Gases; D047428:Protein Kinase Inhibitors; D016601:RNA-Binding Proteins; D048049:Extracellular Signal-Regulated MAP Kinases