title: Deoxyribonucleic Acid copy number aberrations in vasospastic angina patients using an array comparative genomic hybridization.
abstract: OBJECTIVE
  Vasospastic angina (VA) is a specific type of coronary artery disease and develops as a result of coronary artery spasm. Recently, a few studies have revealed that VA caused by coronary artery spasm is related to genetic traits. The objective of this study was to use the recently developed technique of array comparative genomic hybridization (CGH) to screen the genetic aberrations of VA.
  
  
  METHODS
  To identify candidate genes that might be causally involved in the pathogenesis of VA, genomic deoxyribonucleic acids were extracted from whole blood of 28 patients with VA who presented at Department of Cardiology at Seoul St. Mary's Hospital, Seoul, Korea. The copy number profiles of these patients was then analyzed using array CGH and reverse transcriptase (RT) quantitative polymerase chain reaction (PCR).
  
  
  RESULTS
  Array CGH revealed gains in 31 different regions, with losses in the 4q35.2, 7q22.1, 10q26.3, 15q11.2, 16p13.11, 17p11.2 and 19q13.3 regions (more than 32% aberration in these regions). Several loci were found to be frequently including gains of 5p and 11q (50% of samples). The most common losses were found in 7q (54% of samples). Copy number aberrations in chromosomal regions were detected and corresponding genes were confirmed by RT quantitative PCR. The fold change levels were highest in the CTDP1 (18q23), HDAC10 (22q13.33), KCNQ1 (11p15.5-p15.4), NINJ2 (12p13.33), NOTCH2 (1p12-p11.2), PCSK6 (15q26.3), SDHA (5p15.33), and MUC17 (7q22.1) genes.
  
  
  CONCLUSIONS
  Many candidate chromosomal regions that might be related to the pathogenesis of VA were detected by array CGH and should be systematically investigated to establish the causative and specific genes for VA.
authors.affiliation: Cardiovascular Center and Cardiology Division, College of Medicine, The Catholic University of Korea, Seoul, Korea.
authors.forename: Suk Min
authors.initials: SM
authors.lastname: Seo
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Koh
authors.forename: Hae Ok
authors.initials: HO
authors.lastname: Jung
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Choi
authors.forename: Pum Joon
authors.initials: PJ
authors.lastname: Kim
authors.forename: Sang Hong
authors.initials: SH
authors.lastname: Baek
authors.forename: Ho-Joong
authors.initials: HJ
authors.lastname: Youn
authors.forename: Kweon-Haeng
authors.initials: KH
authors.lastname: Lee
authors.forename: Ki-Bae
authors.initials: KB
authors.lastname: Seung