title: Adsorptive modulation of inflammatory mediators dampens endothelial cell activation.
abstract: OBJECTIVE
  In this study, the effect of specific or selective adsorption of inflammatory mediators on endothelial activation was assessed.
  
  
  METHODS
  Conditioned medium was obtained by stimulation of monocytic THP-1 cells with lipopolysaccharide and treated either with an adsorbent specific for tumour necrosis factor-α or with an albumin-coated polystyrene-divinylbenzene copolymer which selectively binds a range of cytokines. Thereafter, the conditioned medium was applied to endothelial cells in culture.
  
  
  RESULTS
  Adsorption of inflammatory mediators resulted in significantly decreased endothelial cell activation, as shown by reduced interleukin (IL)-6 and IL-8 secretion from endothelial cells as well as reduced surface expression of the adhesion molecules intercellular adhesion molecule-1 and E-selectin. The effect was more pronounced the earlier the mediator modulation was performed.
  
  
  CONCLUSIONS
  Adsorptive modulation of inflammatory mediators dampens endothelial cell activation and may thus be beneficial as supportive therapy in sepsis.
authors.affiliation: Department for Clinical Medicine and Biotechnology, Danube University Krems, Austria.
authors.forename: Anita
authors.initials: A
authors.lastname: Schildberger
authors.forename: Tanja
authors.initials: T
authors.lastname: Buchacher
authors.forename: Viktoria
authors.initials: V
authors.lastname: Weber
authors.forename: Dieter
authors.initials: D
authors.lastname: Falkenhagen
mesh_terms: D000327:Adsorption / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D018836:Inflammation Mediators; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha