title: Characterization of in vitro hemostatic peptide effects by thromboelastography.
abstract: In this study, we validated a thromboelastography (TEG) method to evaluate the hemostatic effects of 3 peptides. The first peptide is an ideal amphipathic peptide composed of 22 leucine and lysine in a ratio of 2:1. At a very low concentration, the peptide had a procoagulant effect shown by decreases in reaction time (R) and coagulation time (K) but was impaired by a decrease in maximum amplitude (MA). At higher concentrations, the peptide had an anticoagulant effect. The α angle was minimally affected by the peptide. The second peptide is melittin derived from bee venom. Melittin showed procoagulant effects reflected by a decrease in clotting time but led to lower MA. The third peptide derived from fibrinogen γ chain promoted hemostasis only at an optimal concentration and became anticoagulant at a higher concentration. The hemostatic mechanisms of each peptide were discussed. Our study would facilitate further development of peptides for either hemorrhage control or thrombosis treatment.
authors.affiliation: Defence Research and Development Canada, Toronto, Ontario, Canada. henry.peng@drdc-rddc.gc.ca
authors.forename: Henry T
authors.initials: HT
authors.lastname: Peng
authors.forename: Mark D
authors.initials: MD
authors.lastname: Blostein
authors.forename: Pang N
authors.initials: PN
authors.lastname: Shek
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005340:Fibrinogen / Q000737:chemistry / Q000494:pharmacology*; D006490:Hemostatics / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008555:Melitten / Q000737:chemistry / Q000494:pharmacology*; D013916:Thrombelastography
chemical_list: D006490:Hemostatics; D008555:Melitten; D005340:Fibrinogen