title: Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese.
abstract: Rapid diagnosis of individuals involved in brucellosis outbreaks can sometimes be difficult with conventional microbiological techniques. We analyzed, for the first time, the diagnostic yield of a real-time polymerase chain reaction (PCR) assay in a family outbreak of brucellosis due to consumption of unpasteurized goat cheese. PCR correctly identified all symptomatic cases.
authors.affiliation: Infectious Diseases Service, Carlos Haya University Hospital, Málaga, Spain.
authors.forename: Juan D
authors.initials: JD
authors.lastname: Colmenero
authors.forename: Encarnación
authors.initials: E
authors.lastname: Clavijo
authors.forename: Pilar
authors.initials: P
authors.lastname: Morata
authors.forename: María J
authors.initials: MJ
authors.lastname: Bravo
authors.forename: María I
authors.initials: MI
authors.lastname: Queipo-Ortuño
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D017347:Brucella melitensis / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis* / Q000453:epidemiology*; D002611:Cheese / Q000382:microbiology*; D002648:Child; D004196:Disease Outbreaks; D004435:Eating; D005192:Family Health; D005260:Female; D005798:Genes, Bacterial; D006041:Goats; D006801:Humans; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult