title: Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells in vitro.
abstract: Bone graft substitutes (BGS) are widely used in clinical practice. For stem cellbased approaches to bone tissue engineering BGS need to show sufficient biocompatibility in the in vitro setting. This study was designed to demonstrate the influence of six different BGS on the proliferation and metabolic activity of porcine mesenchymal multilineage stem cells (pMSC) in vitro. Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells. Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation. In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone.
authors.affiliation: Department of Oral and Craniomacillofacial Surgey, University Hospital Schleswig-Holstein Campus Kiel, Germany. c.e.zimmermann@gmx.de
authors.forename: C E
authors.initials: CE
authors.lastname: Zimmermann
authors.forename: M
authors.initials: M
authors.lastname: Gierloff
authors.forename: J
authors.initials: J
authors.lastname: Hedderich
authors.forename: Y
authors.initials: Y
authors.lastname: Açil
authors.forename: J
authors.initials: J
authors.lastname: Wiltfang
authors.forename: H
authors.initials: H
authors.lastname: Terheyden
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D019070:Cell Lineage; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature; D019737:Transplants
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes