title: Localized surface plasmon resonance interfaces coated with poly[3-(pyrrolyl)carboxylic acid] for histidine-tagged peptide sensing.
abstract: The paper reports on a novel localized surface plasmon resonance (LSPR) substrate architecture for the immobilization and detection of histidine-tagged peptides. The LSPR interface consists of an ITO (indium tin oxide) substrate coated with gold nanostructures. The latter are obtained by thermal deposition of a thin (2 nm thick) gold film followed by post-annealing at 500 °C. The LSPR interface was coated with poly[3-(pyrrolyl)carboxylic acid] thin films using electrochemical means. The ability of the LSPR interfaces coated with poly[3-(pyrrolyl)carboxylic acid] to chelate copper ions was investigated. Once loaded with metal ions, the modified LSPR interface was able to bind specifically to histidine-tagged peptides. The binding process was followed using LSPR.
authors.affiliation: UDTS, 2, Bd Frantz-Fanon, B.P. 140 Alger-7 merveilles, 16200 Algiers, Algeria.
authors.forename: Fatma-Zohra
authors.initials: FZ
authors.lastname: Tighilt
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Subramanian
authors.forename: Naima
authors.initials: N
authors.lastname: Belhaneche-Bensemra
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Noureddine
authors.initials: N
authors.lastname: Gabouze
authors.forename: Sabrina
authors.initials: S
authors.lastname: Sam
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006639:Histidine / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry*; D010455:Peptides / Q000032:analysis*; D011758:Pyrroles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D002264:Carboxylic Acids; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D010455:Peptides; D011758:Pyrroles; D017971:Tin Compounds; C569491:poly(3-(pyrrolyl)carboxylic acid); D006639:Histidine; C109984:indium tin oxide; D006046:Gold; D003300:Copper