title: The glycan shield of HIV is predominantly oligomannose independently of production system or viral clade.
abstract: The N-linked oligomannose glycans of HIV gp120 are a target for both microbicide and vaccine design. The extent of cross-clade conservation of HIV oligomannose glycans is therefore a critical consideration for the development of HIV prophylaxes. We measured the oligomannose content of virion-associated gp120 from primary virus from PBMCs for a range of viral isolates and showed cross-clade elevation (62-79%) of these glycans relative to recombinant, monomeric gp120 (∼30%). We also confirmed that pseudoviral production systems can give rise to notably elevated gp120 oligomannose levels (∼98%), compared to gp120 derived from a single-plasmid viral system using the HIV(LAI) backbone (56%). This study highlights differences in glycosylation between virion-associated and recombinant gp120.
authors.affiliation: Department of Biochemistry, Oxford Glycobiology Institute, University of Oxford, Oxford, United Kingdom.
authors.forename: Camille
authors.initials: C
authors.lastname: Bonomelli
authors.forename: Katie J
authors.initials: KJ
authors.lastname: Doores
authors.forename: D Cameron
authors.initials: DC
authors.lastname: Dunlop
authors.forename: Victoria
authors.initials: V
authors.lastname: Thaney
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Dwek
authors.forename: Dennis R
authors.initials: DR
authors.lastname: Burton
authors.forename: Max
authors.initials: M
authors.lastname: Crispin
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Scanlan
mesh_terms: D002478:Cells, Cultured; D006031:Glycosylation; D057809:HEK293 Cells; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D006801:Humans; D008358:Mannose / Q000737:chemistry / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014771:Virion / Q000737:chemistry / Q000378:metabolism
chemical_list: D015699:HIV Envelope Protein gp120; D009844:Oligosaccharides; D011134:Polysaccharides; D011994:Recombinant Proteins; D008358:Mannose