title: Expansion and delivery of adipose-derived mesenchymal stem cells on three microcarriers for soft tissue regeneration.
abstract: Cell/microcarrier combinations can be injected to repair tissue defects, but whether currently available microcarriers can be utilized to repair different tissue defects remains unknown. Here, we compared the suitability of fabricated micronized acellular dermal matrix (MADM), micronized small intestinal submucosa (MSIS), and gelatin microspheres as expansion and delivery scaffolds for adipose-derived mesenchymal stem cells (ADSCs). The results of MTS assay, scanning electron microscopy (SEM), and flow cytometry suggested that the three microcarriers all have good biocompatibility. Quantitative polymerase chain reaction revealed enhanced epidermal growth factor, vascular endothelial growth factor, basal fibroblast growth factor, and transforming growth factor-β expression levels after ADSCs had been cultured on MADM or MSIS for 5 days. After culturing ADSCs on microcarriers in osteogenic medium for 7 days, the expression levels of bone formation-related genes were enhanced. ADSC/microcarrier treatment accelerated wound closure. The ADSC/MADM and ADSC/MSIS combinations retained more of the original implant volume at 1 month postimplantation than ADSC/gelatin microspheres combination in soft-tissue augmentation studies. All implants displayed fibroblast and capillary vessel infiltrations; but ectopic bone formation did not occur, and the calvarial defect repair results were unfavorable. Our study demonstrates the potential utility of these microcarriers not only as a cell-culture substrate but also as a cell-transplantation vehicle for skin regeneration and soft-tissue reconstruction.
authors.affiliation: Department of Oral Histology and Pathology, School of Stomatology, The Fourth Military Medical University, Xi'an, PR China.
authors.forename: Yalei
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Yan
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Shiyu
authors.initials: S
authors.lastname: Liu
authors.forename: Xinhui
authors.initials: X
authors.lastname: Huang
authors.forename: Hailang
authors.initials: H
authors.lastname: Luo
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002828:Choristoma / Q000473:pathology; D020405:Dermis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009928:Organ Specificity / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012038:Regeneration / Q000502:physiology*; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D000469:Alkaline Phosphatase