title: Bisphenol-A suppresses neurite extension due to inhibition of phosphorylation of mitogen-activated protein kinase in PC12 cells.
abstract: An endocrine disrupter, bisphenol-A is widely used in the production of plastics and coatings. Recently, it was reported that bisphenol-A affected neurotransmitters in the mammalian brain. On the basis of these reports, it was considered that bisphenol-A affected neuronal differentiation. In this study, the morphological changes in nerve growth factor (NFG)-induced differentiation caused by bisphenol-A were confirmed using a PC12 cell system. When a low concentration of bisphenol-A was added to medium containing NGF, it inhibited neurite extension. In addition, to clarify whether bisphenol-A affects the early and late stages of the NGF-signaling pathway in cell differentiation, changes of phosphorylation of MAP kinases and cAMP-response element binding protein (CREB) in PC12 cells treated with and without BPA in medium containing NGF were investigated using western blot analysis. As results, bisphenol-A significantly inhibited phosphorylation of CREB and ERK1/2 MAPK.
authors.affiliation: Environmental Adaptation Science, Division of Environmental Development, Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Seki
authors.forename: Miho
authors.initials: M
authors.lastname: Aoki
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Hosokawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Runa
authors.initials: R
authors.lastname: Masuma
authors.forename: Miyako
authors.initials: M
authors.lastname: Komori
authors.forename: Masaaki
authors.initials: M
authors.lastname: Kurasaki
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D010636:Phenols / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017362:Cyclic AMP Response Element-Binding Protein; D052244:Endocrine Disruptors; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases; D020932:Nerve Growth Factor; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds