title: A comparative study on the effectiveness of cis- and trans-form of cinnamic acid treatments for inhibiting invasive activity of human lung adenocarcinoma cells.
abstract: Lung cancer is the major cause of tumor-related death, and approximately 70% of lung cancer patients die from metastasis. Evidence that phenolic compounds may have a potential inhibitory effect on cancer invasion and metastasis is increasingly being reported in the scientific literature. Cinnamic acid is a member of phenolics which having several isoforms in nature. The trans-cinnamic acid (t-CA) has been investigated extensively for its potential pharmacological effects whereas the study of cis-cinnamic acid (c-CA) is limited because pure c-CA was hard to obtain. We had developed a practicable method previously to transform and obtain pure c-CA, and the pure compound was used to evaluate the anti-invasive effect on human adenocarcinoma A549 cells. The A549 cells were treated with 0-200 μM of c-CA and t-CA in the presence of 200 nM phorbol-12-myristate-13-acetate (PMA) at 37 °C for 24 h, and matrix metalloproteinase (MMP)-2, MMP-9, adhesive, migratory, and invasive activities of the cells were determined. The results showed that the treatment of c-CA and t-CA dose-dependently reduced the PMA-induced MMP-2 and -9 activities but without significant effect on the adhesive activity of cells. The PMA-induced motility was suppressed in a dose-dependent manner by a 24-h treatment with c-CA and t-CA. The invasive ability was significantly (p<0.05) reduced to 68% and 65%, respectively, relative to PMA treatment alone after treatment of PMA-treated A549 cells with either 50 μM c-CA or 100 μM t-CA for 24 h. The results suggest that both of the c-CA and t-CA are inhibitors for invasion of A549 cells and the activity of c-CA seems to be higher than t-CA.
authors.affiliation: Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Rd, Taichung 40227, Taiwan.
authors.forename: Gow-Chin
authors.initials: GC
authors.lastname: Yen
authors.forename: Yen-Ling
authors.initials: YL
authors.lastname: Chen
authors.forename: Fang-Ming
authors.initials: FM
authors.lastname: Sun
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Chiang
authors.forename: Shiau-Huei
authors.initials: SH
authors.lastname: Lu
authors.forename: Chia-Jui
authors.initials: CJ
authors.lastname: Weng
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008954:Models, Biological; D009361:Neoplasm Invasiveness; D013237:Stereoisomerism; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D002934:Cinnamates; C029010:cinnamic acid; C016658:phorbolol myristate acetate; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate