title: Validated high-performance liquid chromatographic enantioseparation of selenomethionine using isothiocyanate based chiral derivatizing reagents.
abstract: A high-performance liquid chromatographic (HPLC) method for enantioseparation of selenomethionine (SeMet) was developed using two isothiocyanate-based chiral derivatizing reagents [(R)-methyl benzyl isothiocyanate (MBIC) and (S)-1-(1-naphthyl) ethyl isothiocyanate (NEIC)] and UV detection. Diastereomers of selenomethionine were synthesized either via stirring (using MBIC) or by microwave irradiation (using NEIC). Derivatization conditions were optimized and the synthesized diastereomers were successfully resolved using triethyl ammonium phosphate buffer and acetonitrile on a reversed-phase column. The method was validated for accuracy, precision and limit of detection. The mechanism of separation is also discussed.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India. rbushfcy@iitr.ernet.in
authors.forename: Ravi
authors.initials: R
authors.lastname: Bhushan
authors.forename: Rituraj
authors.initials: R
authors.lastname: Dubey
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017879:Isothiocyanates / Q000737:chemistry; D057230:Limit of Detection; D012645:Selenomethionine / Q000302:isolation & purification*; D013237:Stereoisomerism
chemical_list: D017879:Isothiocyanates; C037152:isothiocyanic acid; D012645:Selenomethionine