title: [Effects of enzymatic bone powder on calcium absorption and bone density in rats].
abstract: OBJECTIVE
  To identify the influence of enzymatic cattle bone, enzymatic fish bone and ultrafine-enzymatic cattle bone on apparent calcium absorption rate and bone density in rats.
  
  
  METHODS
  Female SD rats were randomly divided into 9 groups with 10 rats in each group, and were fed with low- and high-enzymatic cattle bone diet, low- and high-enzymatic fish bone diet, low-and high-ultrafine-enzymatic cattle bone diet, low- and high-calcium carbonate diet and low calcium control diet for 13 weeks. The calcium content of the low and high experimental groups was 500 mg/100 g and 1000 mg/100 g, and the calcium content of the control group was 100 mg/100 g. 3-day calcium metabolic experiment was conducted by the end of the 3rd week. Bone density was assayed by the end of the 13th week.
  
  
  RESULTS
  The apparent calcium absorption rate was related to the calcium content in diet in a range of 60.69% -65.84% in the low dose groups and 34.00% - 44.06% in the high dose groups. The apparent calcium absorption rate in experimental groups was obviously lower than that in the low calcium control group (P < 0.05). Bone density of rats in enzymatic cattle bone, enzymatic fish bone and ultrafine-enzymatic cattle bone groups were obviously higher than that in calcium carbonate group at the same calcium content in diet (P < 0.05).
  
  
  CONCLUSIONS
  According to the results of calcium apparent absorption rate and bone density, enzymatic cattle bone, enzymatic fish bone, ultrafine-enzymatic cattle bone, and calcium carbonate could be used as sources of calcium.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Ma
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhu
authors.forename: Xuejun
authors.initials: X
authors.lastname: Liang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Long
authors.forename: Na
authors.initials: N
authors.lastname: Wang
authors.forename: Weirui
authors.initials: W
authors.lastname: Zhang
authors.forename: Hongyun
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000493:pharmacokinetics* / Q000494:pharmacology; D002136:Calcium, Dietary / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002417:Cattle; D019587:Dietary Supplements; D005260:Female; D005399:Fishes; D007408:Intestinal Absorption; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002136:Calcium, Dietary; D002118:Calcium