title: In vivo measurement of glioma-induced vascular permeability.
abstract: The normal blood-brain barrier (BBB) consists of tight interendothelial cell junctions and adjacent astrocyte end feet separated by a basal lamina surrounding the endothelium. The interactions between the different cell types of BBB are disrupted in distinct patterns in the microenvironment of glioma. Malignant gliomas infiltrate the surrounding normal brain parenchyma; a process associated with vascular permeability (VP) and breakdown of the BBB. Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma.
authors.affiliation: Department of Surgery, University of California San Diego, San Diego, CA, USA.
authors.forename: Jisook
authors.initials: J
authors.lastname: Lee
authors.forename: Andrew
authors.initials: A
authors.lastname: Baird
authors.forename: Brian P
authors.initials: BP
authors.lastname: Eliceiri
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D052160:Benzothiazoles / Q000032:analysis; D001812:Blood-Brain Barrier / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002199:Capillary Permeability; D003001:Cloning, Molecular; D003911:Dextrans / Q000032:analysis; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000032:analysis; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D057967:Infusions, Intraventricular; D016086:Lentivirus / Q000235:genetics; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D051379:Mice; D008845:Microinjections / Q000379:methods; D057054:Molecular Imaging; D013238:Stereotaxic Techniques; D019108:Tight Junctions / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays / Q000379:methods*
chemical_list: D052160:Benzothiazoles; C532924:D-luciferin; D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate