title: Separation of a set of peptide sequence isomers using differential ion mobility spectrometry.
abstract: Protein identification in bottom-up proteomics requires disentangling isomers of proteolytic peptides, a major class of which are sequence inversions. Their separation using ion mobility spectrometry (IMS) has been limited to isomeric pairs. Here we demonstrate baseline separation of all seven 8-mer tryptic peptide isomers using differential IMS. Evaluation of peak capacity implies that even larger libraries should be resolved for heavier peptides with higher charge states.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Shvartsburg
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Creese
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000595:Amino Acid Sequence; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D040901:Proteomics; D014357:Trypsin / Q000378:metabolism
chemical_list: D007477:Ions; D010455:Peptides; D014357:Trypsin