title: A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning.
abstract: A novel cell separation process based on immunoaffinity aqueous two phase systems is presented to isolate and purify CD34(+) stem/progenitor cells directly from the whole umbilical cord blood (UCB). A system, composed of polyethylene glycol and dextran, was evaluated for the selective recovery of CD34(+) cells from UCB. A monoclonal antibody against the CD34 surface antigen was used for the direct partitioning of CD34(+) cells in UCB to the PEG-rich phase. The initial population of CD34(+) cells (0.2% of the initial sample) was enriched to values up to 42% in a single partitioning step, while the majority of contaminant cells were partitioned to the dextran-rich phase (1.37 × 10(-2) < K(P) < 2.76 × 10(-2)). This novel selection method allowed a recovery yield of 95% of CD34(+) cells with a purification factor of 245 and is expected to pave a new way to purify hematopoietic stem/progenitor cells for use in a variety of clinical settings.
authors.affiliation: Department of Bioengineering, Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Technical University of Lisbon, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
authors.forename: António F
authors.initials: AF
authors.lastname: Sousa
authors.forename: Pedro Z
authors.initials: PZ
authors.lastname: Andrade
authors.forename: Roksana M
authors.initials: RM
authors.lastname: Pirzgalska
authors.forename: Teresa M
authors.initials: TM
authors.lastname: Galhoz
authors.forename: Ana M
authors.initials: AM
authors.lastname: Azevedo
authors.forename: Cláudia L
authors.initials: CL
authors.lastname: da Silva
authors.forename: M Raquel
authors.initials: MR
authors.lastname: Aires-Barros
authors.forename: Joaquim M S
authors.initials: JM
authors.lastname: Cabral
mesh_terms: D001781:Blood Component Removal / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans