title: Independent localization of MAP2, CaMKIIα and β-actin RNAs in low copy numbers.
abstract: Messenger RNA localization involves the assembly of ribonucleoprotein particles (RNPs) and their subsequent transport along the cytoskeleton to their final destination. Here, we provide new evidence that microtubule-associated protein 2 (MAP2), calcium/calmodulin-dependent protein kinase II (CaMKIIα) and β-actin RNAs localize to dendrites in distinct RNPs, which contain--unexpectedly--very few RNA molecules. The number of MAP2 molecules per particle is affected by synaptic activity and Staufen 2, indicating that RNP composition is tightly controlled. Our data suggest that the independent localization of individual RNAs in low copy numbers could contribute to tighter temporal and spatial control of expression in neurons and synapse-specific plasticity.
authors.affiliation: Center for Brain Research, Department of Neuronal Cell Biology, Medical University of Vienna, Spitalgasse 4, Wien 1090, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Mikl
authors.forename: Georgia
authors.initials: G
authors.lastname: Vendra
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kiebler
mesh_terms: D000199:Actins / Q000235:genetics*; D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics*; D003712:Dendrites / Q000378:metabolism; D005786:Gene Expression Regulation; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D000199:Actins; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C492188:Stau2 protein, rat; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2