title: Enantioselective determination of 3-hydroxybutyrate in the tissues of normal and streptozotocin-induced diabetic rats of different ages.
abstract: L-3-Hydroxybutyrate (3HB) and D-3HB are enantiomers that exist in various rat tissues, and the ratio of the 2 compounds is of importance since it may affect glucose utilization in cardiomyocytes. In this study, we determined the concentrations of L-3HB and D-3HB in the tissues of normal and streptozotocin (STZ)-induced diabetic rats of different ages by column-switching high-performance liquid chromatography using a fluorescence detection system. In normal rats, the levels of L-3HB peaked at 8 weeks of age in the cerebrum, liver, spleen, lung, kidney, adrenal gland, and heart and then decreased afterwards. The concentrations of L-3HB were the highest in the heart, with 26.24±13.74 μmol/mg protein. In addition, there was an increase in the levels of (D+L)-3HB, D-3HB, and L-3HB in the tissues of diabetic rats with time, whereas the ratios of L-3HB to (D+L)-3HB declined (46.44% vs. 21.03%, P<0.05, in heart tissue after 24 weeks of STZ treatment). Both the concentration and the ratio of L-3HB may be associated with disease conditions, and the determination of L-3HB may help clarify the role of L-3HB under physiological and pathological conditions.
authors.affiliation: School of Pharmacy, College of Pharmacy, Taipei Medical University, No. 250 Wuxing St., Taipei 11031, Taiwan.
authors.forename: Wei-Yu
authors.initials: WY
authors.lastname: Hsu
authors.forename: Chen-Yi
authors.initials: CY
authors.lastname: Kuo
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukushima
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Imai
authors.forename: Chien-Ming
authors.initials: CM
authors.lastname: Chen
authors.forename: Pen-Yuan
authors.initials: PY
authors.lastname: Lin
authors.forename: Jen-Ai
authors.initials: JA
authors.lastname: Lee
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D013237:Stereoisomerism
chemical_list: D001786:Blood Glucose; D020155:3-Hydroxybutyric Acid