title: [New feasibility of using a tissue culture technique in diagnostic oncomorphology in case of neuroblastoma and breast cancer].
abstract: The paper gives the results of investigating the cultured tissues from patients with breast cancer (BC) and those with neuroblastoma, by applying both the traditional studies and molecular genetic techniques (FISH). The cultures from the patients with neuroblastoma represented as three cell types--N, S, and I. There are certain similarities between the cell composition in the culture medium and the histological structure of the tumor. Mature neoplasms of the ganglioneuroma type contain S-type cells only while intermediate type I cells are a predominant element of immature neuroblastomas. The presence of a considerable number of I cells in the explants appears to suggest a poor prognosis even when the tumor has a comparatively mature structure as a whole. The results of FISH on explant cells presented a means of detecting a broad range of points that are not recorded on histologic specimens--amplification on the metaphase plate, localization of an amplified product, amplification on one chromosome, and double acentric chromosomes. The results of cultivation of BC cells are difficult to systematize although they offer the advantage of performing the FISH reaction. The authors recommend that the tissue culture method should be more extensively used in the day-to-day diagnostic work of pathology laboratories.
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Tabakov
authors.forename: A M
authors.initials: AM
authors.lastname: Stroganova
authors.forename: V V
authors.initials: VV
authors.lastname: Chestkov
authors.forename: A I
authors.initials: AI
authors.lastname: Karseladze
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D009447:Neuroblastoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D011379:Prognosis; D046509:Tissue Culture Techniques / Q000379:methods*