title: IgE responsiveness to Dermatophagoides farinae in West Highland white terrier dogs is associated with region on CFA35.
abstract: Immunoglobulin E (IgE)-mediated hypersensitivity against environmental allergens, commonly including Dermatophagoides farinae, is associated with atopic diseases in both humans and dogs. We have recently identified a family of clinically healthy West Highland white terriers (WHWTs) with high-serum D. farinae-IgE levels. In this study, we investigated the genetic mechanism controlling IgE responsiveness in dogs by performing a genome-wide association study (GWAS) using the Affymetrix V2 Dog SNP array in 31 high-IgE and 24 low-IgE responder WHWTs. A gene-dropping simulation method, using SIB-PAIR software, showed significant allelic association between serum D. farinae-specific IgE levels and a 2.3-Mb area on CFA35 (best empirical P = 1 × 10(-5)). A nearby candidate gene, CD83, encodes a protein which has important immunological functions in antigen presentation and regulation of humoral immune responses. We sequenced this gene in 2 high-IgE responders and 2 low-IgE responders and identified an intronic polymorphic repeat sequence with a predicted functional effect, but the association was insufficient to explain the GWAS association signal in this population (P = 1 × 10(-3)). Further studies are necessary to investigate the significance of these findings for IgE responsiveness and atopic disease in the dog.
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton, Queensland 4343, Australia.
authors.forename: Joana Barros
authors.initials: JB
authors.lastname: Roque
authors.forename: Caroline Ann
authors.initials: CA
authors.lastname: O'Leary
authors.forename: David Lorenzo
authors.initials: DL
authors.lastname: Duffy
authors.forename: Myat
authors.initials: M
authors.lastname: Kyaw-Tanner
authors.forename: Melanie
authors.initials: M
authors.lastname: Latter
authors.forename: Kenneth
authors.initials: K
authors.lastname: Mason
authors.forename: Linda
authors.initials: L
authors.lastname: Vogelnest
authors.forename: Michael
authors.initials: M
authors.lastname: Shipstone
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D040001:Dermatophagoides farinae / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D056426:Genetic Loci / Q000235:genetics*; D055106:Genome-Wide Association Study; D006969:Hypersensitivity, Immediate / Q000469:parasitology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood; D007136:Immunoglobulins / Q000235:genetics; D016014:Linear Models; D008562:Membrane Glycoproteins / Q000235:genetics; D008924:Mite Infestations / Q000276:immunology / Q000662:veterinary*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D017931:DNA Primers; D007073:Immunoglobulin E; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen