title: Tissue distribution and expression of signaling lymphocyte activation molecule receptor to peste des petits ruminant virus in goats detected by real-time PCR.
abstract: In the present study, we investigated the tissue distribution and expression of signaling lymphocyte activation molecule (SLAM) in 40 tissues and organs of goats by real-time RT-PCR, in order to determine the role of these receptors in tissue tropism. SLAM mRNA was detected in all the samples investigated. The expression of SLAM mRNA was detected at high levels in spleen, mesenteric lymph node, hilar lymph node, mandibular lymph node, superficial cervical lymph node, nasal mucosa, duodenum, heart, gallbladder, thymus and blood; this is similar to the tissue tropism of peste des petits ruminant virus. However, it was surprising that expression of SLAM was low in lungs, colon and rectum which are the major sites of replication of PPRV. In addition, very low levels were detected in larynx, tongue and esophagus, which suggest the possible presence of an alternative receptor for PPRV. This study provided the first data on caprine SLAM for use in further studies of the pathogenesis of PPRV in goats.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Gansu Provincial Engineering and Technique Research Centre on Biological Detection, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Yanchangpu, Chengguan District, Lanzhou 730046, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Meng
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Dou
authors.forename: Junjun
authors.initials: J
authors.lastname: Zhai
authors.forename: Hairui
authors.initials: H
authors.lastname: Zhang
authors.forename: Fengchao
authors.initials: F
authors.lastname: Yan
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Shi
authors.forename: Xuelong
authors.initials: X
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015511:Goat Diseases / Q000235:genetics* / Q000821:virology*; D006041:Goats / Q000235:genetics / Q000821:virology*; D029021:Peste-des-Petits-Ruminants / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D012015:Reference Standards; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D014018:Tissue Distribution / Q000235:genetics
chemical_list: D015703:Antigens, CD; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1