title: Three-dimensional visualization of bioactive glass-bone integration in a rabbit tibia model using synchrotron X-ray microcomputed tomography.
abstract: Synchrotron X-ray microcomputed tomography (SR microCT), with a micron resolution, was used to evaluate the osteoconduction and osteointegration by borate bioactive glass after implantation 12 weeks in a rabbit tibia model. The study focused on the biomaterial-bone interface. Results from SR microCT two-dimensional and three-dimensional (3D) reconstructions provided precise imaging of the biomaterial-bone integration and detailed microarchitecture of both the bone-like glass graft and the newly formed trabecular bone. Osteoconduction, the formation of new trabecular bone within a tibia defect, occurred only in the tibiae implanted with teicoplanin-loaded borate glass but not in those with teicoplanin-loaded CaSO(4) beads, indicating the excellent biocompatibility of the glass implants. 3D reconstruction of the tibiae also showed the infiltration of vascular tissue in both the bioactive glass graft and the new trabecular bone. This study indicates that SR microCT can serve as a valuable complementary technique for imaging bone repair when using bioactive glass implants.
authors.affiliation: Lawrence Berkeley National Laboratory, Materials Sciences Division, Berkeley, CA 94720, USA. qfu@lbl.gov
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Wenhai
authors.initials: W
authors.lastname: Huang
authors.forename: Weitao
authors.initials: W
authors.lastname: Jia
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Rahaman
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Antoni P
authors.initials: AP
authors.lastname: Tomsia
mesh_terms: D000818:Animals; D005898:Glass / Q000737:chemistry*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D020007:Implants, Experimental; D023421:Models, Animal; D011817:Rabbits; D017356:Synchrotrons; D017334:Teicoplanin / Q000494:pharmacology; D013977:Tibia / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: C570888:Na2O-K2O-MgO-CaO-B2O3-P2O5 glass; D017334:Teicoplanin