title: Statistically sound calibration curves for chromatographic methods involving negative response data.
abstract: A statistical procedure is presented for calibrating chromatographic methods that generate negative chromatographic peaks. The technique is illustrated via data collected during the ion-chromatographic analysis of the ammonium ion in methansulfonic-acid solutions. A peak-integration protocol is explained and subsequent regression data are presented. For the concentration range of 1 to 5 ppb, a quadratic model could be fitted to the calibration data, using ordinary least squares as the fitting technique. The precision was approximately ± 0.5 ppb, at 95% confidence.
authors.affiliation: Air Liquide-Balazs, Dallas, TX, USA. lynn.vanatta@airliquide.com
authors.forename: L E
authors.initials: LE
authors.lastname: Vanatta
authors.forename: D C
authors.initials: DC
authors.lastname: Cowles
mesh_terms: D002138:Calibration / Q000592:standards; D002852:Chromatography, Ion Exchange / Q000592:standards*; D016018:Least-Squares Analysis; D008698:Mesylates / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012044:Regression Analysis
chemical_list: D008698:Mesylates; D000644:Quaternary Ammonium Compounds; C045880:methanesulfonic acid