title: [Effect of myriocin on the expression of cyclinD1 in high glucose-induced hypertrophy mesangial cells].
abstract: OBJECTIVE
  Myriocin (ISP-1) is a new type of immune inhibitor extracted from cordyceps sinensis. This study was to observe the effects of ISP-1 on the expression of cell cycle regulatory protein D1 (cyclinD1) in high glucose-induced hypertrophy rat glomerular mesangial cells (GMCs).
  
  
  METHODS
  Rat GMCs were cultured in vitro and divided into three groups: high glucose (450 mg/dL D-glucose), normal glucose (100 mg/dL D-glucose, control) and ISP-1 (450 mg/dL D-glucose plus 100 μg/mL ISP-1). The protein expression of cyclinD1 was detected by flow cytometry.
  
  
  RESULTS
  The expression of cyclinD1 in GMCs in the high glucose group increased significantly in a time-dependent manner compared with that in the control group. ISP-1 treatment significantly inhibited the up-regulated expression of cyclinD1 induced by high concentration glucose, and the expression of cyclinD1 was restored to the level of the control group 48 and 72 hrs after ISP-1 treatment.
  
  
  CONCLUSIONS
  High concentration of glucose can up-regulate the expression of cyclinD1 in GMCs. ISP-1 may inhibit the up-regulated expression of cyclinD1, which might contribute to the protective effect of ISP-1 against GMC hypertrophy induced by high glucose.
authors.affiliation: Department of Pediatrics, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Zhao-Hua
authors.initials: ZH
authors.lastname: Xiao
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhou
authors.forename: Heng-Sheng
authors.initials: HS
authors.lastname: Wu
mesh_terms: D000818:Animals; D019938:Cyclin D1 / Q000032:analysis*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D016193:G1 Phase; D005947:Glucose / Q000633:toxicity*; D006984:Hypertrophy; D050527:Mesangial Cells / Q000737:chemistry / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005229:Fatty Acids, Monounsaturated; D019938:Cyclin D1; D005947:Glucose; C001996:thermozymocidin