title: Epigallocatechin gallate changes mRNA expression level of genes involved in cholesterol metabolism in hepatocytes.
abstract: Catechins, compounds derived from green tea, have been shown to improve cholesterol metabolism in animal studies, but the molecular mechanisms underlying this function have not been fully understood. We performed DNA microarray analysis in order to clarify the effects of epigallocatechin gallate (EGCG), the dominant catechin in green tea, on cholesterol metabolism in HepG2 hepatocytes. This revealed that the expression levels of several genes related to cholesterol metabolism, including the LDL receptor, were changed by EGCG treatment. Using a real-time PCR technique, we confirmed that EGCG treatment up-regulated mRNA expression level of the LDL receptor. Moreover, EGCG decreased extracellular apoB levels. These findings indicated that EGCG improves cholesterol metabolism through the up-regulation of LDL receptor and also reduces extracellular apoB levels.
authors.affiliation: Department of Applied Life Science, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Saito
authors.forename: Kensei
authors.initials: K
authors.lastname: Morikawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kanamaru
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagaoka
mesh_terms: D000924:Anticholesteremic Agents / Q000494:pharmacology*; D001055:Apolipoproteins B / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism*; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000924:Anticholesteremic Agents; D001055:Apolipoproteins B; D012333:RNA, Messenger; D011973:Receptors, LDL; C544617:gallocatechin-7-gallate; D002392:Catechin; D002784:Cholesterol