title: Differentiated skeletal cells contribute to blastema formation during zebrafish fin regeneration.
abstract: The origin of cells that generate the blastema following appendage amputation has been a long-standing question in epimorphic regeneration studies. The blastema is thought to originate from either stem (or progenitor) cells or differentiated cells of various tissues that undergo dedifferentiation. Here, we investigate the origin of cells that contribute to the regeneration of zebrafish caudal fin skeletal elements. We provide evidence that the process of lepidotrichia (bony rays) regeneration is initiated as early as 24 hours post-amputation and that differentiated scleroblasts acquire a proliferative state, detach from the lepidotrichia surface, migrate distally, integrate into the blastema and dedifferentiate. These findings provide novel insights into the origin of cells in epimorphic appendage regeneration in zebrafish and suggest conservation of regeneration mechanisms between fish and amphibians.
authors.affiliation: Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Sousa
authors.forename: Nuno
authors.initials: N
authors.lastname: Afonso
authors.forename: Anabela
authors.initials: A
authors.lastname: Bensimon-Brito
authors.forename: Mariana
authors.initials: M
authors.lastname: Fonseca
authors.forename: Mariana
authors.initials: M
authors.lastname: Simões
authors.forename: Joaquín
authors.initials: J
authors.lastname: Leon
authors.forename: Henry
authors.initials: H
authors.lastname: Roehl
authors.forename: Maria Leonor
authors.initials: ML
authors.lastname: Cancela
authors.forename: António
authors.initials: A
authors.lastname: Jacinto
mesh_terms: D058500:Animal Fins / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D001846:Bone Development / Q000235:genetics; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D054337:Cell Dedifferentiation / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation; D015870:Gene Expression; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D014945:Wound Healing / Q000235:genetics / Q000502:physiology; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins