title: Simple and rapid method for the determination of uric acid-independent antioxidant capacity.
abstract: Determination of the relative contribution of uric acid level increases to the total measured antioxidative activity could be very useful for testing antioxidative products and their effect on human health. The aim of this report is to present a simple spectrophotometric method that combines the measurement of total antioxidative capacity of a sample by ferric reducing/antioxidative power (FRAP) assay, with the uricase-reaction (specific elimination of uric acid), in order to establish and correct for the contribution of uric acid in FRAP values. We measured FRAP values, with (uric acid-independent antioxidant capacity, TAC-UA) and without (total antioxidant capacity, TAC) uricase treatment, and expressed it as μmol/L of uric acid equivalents. In such way, it was possible to determine both total and uric acid-independent antioxidant capacity, plasma uric acid (UA, as the difference between TAC and TAC-UA), and the ratio of the uric acid in total antioxidant capacity (UA/TAC).
authors.affiliation: Department of Cardiology, University Hospital Split, School of Medicine, University of Split, Croatia. darko.duplancic@gmail.com
authors.forename: Darko
authors.initials: D
authors.lastname: Duplancic
authors.forename: Lea
authors.initials: L
authors.lastname: Kukoc-Modun
authors.forename: Darko
authors.initials: D
authors.lastname: Modun
authors.forename: Njegomir
authors.initials: N
authors.lastname: Radic
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D006801:Humans; D033461:Hyperuricemia / Q000097:blood* / Q000503:physiopathology; D010084:Oxidation-Reduction; D013053:Spectrophotometry / Q000379:methods*; D014503:Urate Oxidase / Q000378:metabolism*; D014527:Uric Acid / Q000097:blood*
chemical_list: D000975:Antioxidants; D014527:Uric Acid; D014503:Urate Oxidase; D001205:Ascorbic Acid