title: Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells.
abstract: BACKGROUND
  This study was to investigate the effects of tricalcium silicate (Ca(3)SiO(5)) on proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro.
  
  
  METHODS
  The hDPCs were seeded in culture medium with or without Ca(3)SiO(5) extract and calcium hydroxide (Ca(OH)(2)) extract. Proliferation of the hDPCs was measured by methyl-thiazol-tetrazolium (MTT) assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction by using odontogenic marker genes such as dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP 1), osteocalcin (OC), alkaline phosphatase (ALP), and collagen type I (Col I), which were verified by ALP activity assessment, mineralization assay, and immunocytochemistry staining for dentin sialoprotein (DSP).
  
  
  RESULTS
  The MTT assay showed that hDPCs cultured with Ca(3)SiO(5) extract proliferated more significantly as compared with Ca(OH)(2) extract. Analysis of odontogenic marker genes indicated that Ca(3)SiO(5) enhanced the expression of those genes. Moreover, the extract of Ca(3)SiO(5) stimulated mineralization and increased ALP and DSP production conspicuously.
  
  
  CONCLUSIONS
  These results reveal that Ca(3)SiO(5) can induce the proliferation and odontogenic differentiation of hDPCs in vitro and might be a potential candidate for preparation of a new type of Ca(3)SiO(5-)based cement as a pulp-capping agent.
authors.affiliation: Department of General Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Peng
authors.forename: Weining
authors.initials: W
authors.lastname: Liu
authors.forename: Wanyin
authors.initials: W
authors.lastname: Zhai
authors.forename: Long
authors.initials: L
authors.lastname: Jiang
authors.forename: Lifen
authors.initials: L
authors.lastname: Li
authors.forename: Jiang
authors.initials: J
authors.lastname: Chang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000235:genetics; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000096:biosynthesis / Q000235:genetics; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D009804:Odontoblasts / Q000378:metabolism; D009805:Odontogenesis / Q000187:drug effects* / Q000235:genetics; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D057872:Pulp Capping and Pulpectomy Agents / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D017640:Silicates / Q000494:pharmacology*; D014074:Tooth Calcification / Q000187:drug effects / Q000235:genetics; D055815:Young Adult; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D017610:Calcium Compounds; D002126:Calcium Hydroxide; D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D015675:Osteocalcin; D010750:Phosphoproteins; D057872:Pulp Capping and Pulpectomy Agents; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human; C506393:tricalcium silicate