title: Biophysical and microbiological study of high hydrostatic pressure inactivation of Bovine Viral Diarrheavirus type 1 on serum.
abstract: The effect of high hydrostatic pressure application on fetal bovine serum components and the model microorganism (Bovine Viral Diarrheavirus type 1 NADL strain) was studied at 132 and 220 MPa pressure for 5 min at 25°C. Protein secondary structures were found to be unaffected by an artificial neural network application on the amide I region for both untreated and HHP treated samples. FTIR spectroscopy study of both the HHP-treated and control samples revealed changes in the intensity of some bands in the finger-print region (1500-900 cm(-1)) originating mainly from lipids which are thought to result from changes in the lipoprotein structure. The virus strain lost its infectivity completely after 220 MPa HHP treatments. These results indicate that HHP can be successfully used for inactivation of pestiviruses while leaving structural and functional properties of serum and serum products unaffected.
authors.affiliation: Department of Food Engineering, İzmir Institute of Technology, Urla, 35430 İzmir, Turkey. cagatayceylan@iyte.edu.tr
authors.forename: Cagatay
authors.initials: C
authors.lastname: Ceylan
authors.forename: Feride
authors.initials: F
authors.lastname: Severcan
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozkul
authors.forename: Mete
authors.initials: M
authors.lastname: Severcan
authors.forename: Faruk
authors.initials: F
authors.lastname: Bozoglu
authors.forename: Nusret
authors.initials: N
authors.lastname: Taheri
mesh_terms: D000465:Algorithms; D000818:Animals; D001703:Biophysics; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000737:chemistry*; D006874:Hydrostatic Pressure; D016571:Neural Networks, Computer; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D038301:Virus Inactivation