title: [Feasibility of peptide mass fingerprinting for differential diagnosis of IgA and non-IgA nephropathy].
abstract: OBJECTIVE
  To investigate the feasibility of peptide mass fingerprinting for non-invasive differential diagnosis of IgA nephropathy (IgAN) from the non-IgA nephropathy (IgAN).?
  
  
  METHODS
  According to the results of renal biopsy, 56 patients were divided into IgAN group (n=28) and non-IgAN group (n=28), and peptide mass fingerprints were acquired from these patients using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
  
  
  RESULTS
  Nine different peptides were identified between IgAN and non-IgAN. The two most distinctive differentially expressed peptides, with peptide peak values of 4476.46 and 1968.10, showed area under curve values of 86.18% and 79.77%. Principal component analysis demonstrated that the accumulated explained variance of the first 8 differential peptides reached 95%, suggesting the feasibility of differential diagnosis of IgAN from non-IgAN. Comparison with the Matrix protein database identified the peptide with a relative molecular mass of 5338.08 as a fragment of mucin 4 inform and the 2082.77 peptide as fragment of α1-II type collagen inform.
  
  
  CONCLUSIONS
  MALDI-TOF MS is feasible for differential diagnosis of IgAN and non-IgAN and also has great potentials in the classification of the subtypes of other systemic diseases.
authors.affiliation: Department of Clinical Biochemistry, General Hospital of PLA, Beijing 100853, China. gaojingwang @yahoo.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.forename: Xin-yu
authors.initials: XY
authors.lastname: Wen
authors.forename: Hong-hao
authors.initials: HH
authors.lastname: Lu
authors.forename: Zhen-nan
authors.initials: ZN
authors.lastname: Dong
authors.forename: Ya-ping
authors.initials: YP
authors.lastname: Tian
mesh_terms: D000328:Adult; D003937:Diagnosis, Differential; D005240:Feasibility Studies; D005260:Female; D005922:Glomerulonephritis, IGA / Q000175:diagnosis*; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D010455:Peptides