title: Efficient lactobionic acid production from whey by Pseudomonas taetrolens under pH-shift conditions.
abstract: Lactobionic acid finds applications in the fields of pharmaceuticals, cosmetics and medicine. The production of lactobionic acid from whey by Pseudomonas taetrolens was studied in shake-flasks and in a bioreactor. Shake-flask experiments showed that lactobionic acid was a non-growth associated product. A two-stage pH-shift bioconversion strategy with a pH-uncontrolled above 6.5 during the growth phase and maintained at 6.5 during cumulative production was adopted in bioreactor batch cultures. An inoculation level of 30% promoted high cell culture densities that triggered lactobionic acid production at a rate of 1.12 g/Lh. This methodology displayed efficient bioconversion with cheese whey as an inexpensive substrate for lactobionic acid production.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Oviedo, Faculty of Chemistry, C/Julián Clavería s/n, 33071 Oviedo, Spain.
authors.forename: Saúl
authors.initials: S
authors.lastname: Alonso
authors.forename: Manuel
authors.initials: M
authors.lastname: Rendueles
authors.forename: Mario
authors.initials: M
authors.lastname: Díaz
mesh_terms: D019149:Bioreactors; D002611:Cheese; D004187:Disaccharides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism*
chemical_list: D004187:Disaccharides; C005608:lactobionic acid