title: [In vitro diagnosis and monitoring of Hymenoptera venom hyposensitization].
abstract: Specific immunotherapy (SIT) of hymenoptera venom allergy, in particular, has become an example for the effectiveness of the treatment of IgE-mediated allergic diseases. In vitro diagnostic procedures and the measurement of serum tryptase for risk assessment are well-established in the process of diagnose finding and therapy preparation. For monitoring and validation of the effectiveness of the SIT, however, in vitro diagnostic procedures remain controversial. Potentially useful approaches include detection of specific IgE, specific IgG4, basophile activation - represented by the CD 63 expression - and the lymphocyte proliferation and its IL10 release. Preliminary data suggest that the latter method appear appropriate, whereas the detection of basophile activation did not produce definite results.
authors.affiliation: Hautklinik - Klinik für Dermatologie und Allergologie, Universitätsklinikum der RWTH Aachen, Aachen, Deutschland. mark.neis@post.rwth-aachen.de
authors.forename: M M
authors.initials: MM
authors.lastname: Neis
authors.forename: G
authors.initials: G
authors.lastname: Wurpts
authors.forename: L
authors.initials: L
authors.lastname: Wilbers
authors.forename: H F
authors.initials: HF
authors.lastname: Merk
mesh_terms: D000818:Animals; D015549:Basophil Degranulation Test; D001514:Bee Venoms / Q000008:administration & dosage* / Q000276:immunology*; D003888:Desensitization, Immunologic / Q000379:methods*; D000939:Epitopes / Q000008:administration & dosage* / Q000276:immunology*; D006801:Humans; D006927:Hymenoptera / Q000276:immunology*; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000097:blood; D008213:Lymphocyte Activation / Q000276:immunology; D060149:Tetraspanin 30 / Q000097:blood; D016896:Treatment Outcome; D053802:Tryptases / Q000097:blood; D014862:Wasp Venoms / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D001514:Bee Venoms; C554586:CD63 protein, human; D000939:Epitopes; D007074:Immunoglobulin G; D060149:Tetraspanin 30; D014862:Wasp Venoms; D016753:Interleukin-10; D007073:Immunoglobulin E; D053802:Tryptases