title: Structural modification effects on bioactivities of the novel 15-mer peptide adjuvant.
abstract: Structure-function relationship studies for novel GMDP-peptide mimic, RVPPRYHAKISPMVN (RN-peptide) were performed on array of truncated and 'Ala-scan' analogues. The shortest peptide fragment possessing detectable affinity towards anti-GMDP-antibodies was demonstrated to be PRYH. RN-peptide analogues lacking up to 8 residues at C-terminus were found to retain adjuvant activity with the minimal active peptide being RVPPRYH. Evaluation of Ala-scan analogues highlighted that adjuvant activity is most critically dependent on both arginine residues, but also is sensitive to substitution of K9, I10, S11 and M13 amino acid residues, the functional importance of which was additionally confirmed by testing peptides truncated at both termini.
authors.affiliation: Pushchino Branch of Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow region, 142290, Russian Federation.
authors.forename: A G
authors.initials: AG
authors.lastname: Laman
authors.forename: A O
authors.initials: AO
authors.lastname: Shepelyakovskaya
authors.forename: Kh M
authors.initials: KhM
authors.lastname: Boziev
authors.forename: G V
authors.initials: GV
authors.lastname: Savinov
authors.forename: L K
authors.initials: LK
authors.lastname: Baidakova
authors.forename: A N
authors.initials: AN
authors.lastname: Chulin
authors.forename: I A
authors.initials: IA
authors.lastname: Chulina
authors.forename: T
authors.initials: T
authors.lastname: Korpela
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeyanov
authors.forename: F A
authors.initials: FA
authors.lastname: Brovko
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D019943:Amino Acid Substitution; D000818:Animals; D000906:Antibodies / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006020:Glycopeptides / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D013329:Structure-Activity Relationship
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D006020:Glycopeptides; D010047:Ovalbumin