title: Computational analysis of two species C human adenoviruses provides evidence of a novel virus.
abstract: Human adenovirus C (HAdV-C) species are a common cause of respiratory infections and can occasionally produce severe clinical manifestations. A deeper understanding of the variation and evolution in species HAdV-C is especially important since these viruses, including HAdV-C6, are used as gene delivery vectors for human gene therapy and in other biotechnological applications. Here, the full-genome analysis of the prototype HAdV-C6 and a recently identified virus provisionally termed HAdV-C57 are reported. Although the genomes of all species HAdV-C members are very similar to each other, the E3 region, hexon and fiber (ten proteins total) present a wide range of identity values at the amino acid level. Studies of these viruses in comparison to the other three HAdV-C prototypes (1, 2, and 5) comprise a comprehensive analysis of the diversity and conservation within HAdV-C species. HAdV-C6 contains a recombination event within the constant region of the hexon gene. HAdV-C57 is a recombinant virus with a fiber gene nearly identical to HAdV-C6 and a unique hexon distinguished by its loop 2 motif.
authors.affiliation: Department of Bioinformatics and Computational Biology, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Walsh
authors.forename: Jason
authors.initials: J
authors.lastname: Seto
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Liu
authors.forename: Shoaleh
authors.initials: S
authors.lastname: Dehghan
authors.forename: Nolan R
authors.initials: NR
authors.lastname: Hudson
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Lukashev
authors.forename: Olga
authors.initials: O
authors.lastname: Ivanova
authors.forename: James
authors.initials: J
authors.lastname: Chodosh
authors.forename: David W
authors.initials: DW
authors.lastname: Dyer
authors.forename: Morris S
authors.initials: MS
authors.lastname: Jones
authors.forename: Donald
authors.initials: D
authors.lastname: Seto
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification* / Q000235:genetics*; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral