title: Receptor-binding specificity of the human parainfluenza virus type 1 hemagglutinin-neuraminidase glycoprotein.
abstract: The hemagglutinin-neuraminidase (HN) glycoprotein is utilized by human parainfluenza viruses for binding to the host cell. By the use of glycan array assays, we demonstrate that, in addition to the first catalytic-binding site, the HN of human parainfluenza virus type 1 has a second site for binding covered by N-linked glycan. Our data suggest that attachment of the first site to sialic acid (SA)-linked receptors triggers exposure of the second site. We found that both sites bind to α2-3-linked SAs with a preference for a sialyl-Lewis(x) motif. Binding to α2-3-linked SAs with a sulfated sialyl-Lewis motif as well as to α2-8-linked SAs was unique for the second binding site. Neither site recognizes α2-6-linked oligosaccharides.
authors.affiliation: Department of Infectious Diseases, St Jude Children's Research Hospital, 262 Danny Thomas Place, Memphis, TN 38105-3678, USA. irina.alymova@stjude.org
authors.forename: Irina V
authors.initials: IV
authors.lastname: Alymova
authors.forename: Allen
authors.initials: A
authors.lastname: Portner
authors.forename: Vasiliy P
authors.initials: VP
authors.lastname: Mishin
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: McCullers
authors.forename: Pamela
authors.initials: P
authors.lastname: Freiden
authors.forename: Garry L
authors.initials: GL
authors.lastname: Taylor
mesh_terms: D001386:Azides / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism*; D015707:HN Protein / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006603:Hexuronic Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009154:Mutation / Q000235:genetics*; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism*; D000080506:Sialyl Lewis X Antigen
chemical_list: C486444:4-zido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxyglycerogalacto-2-nonulopyranosic acid; D001386:Azides; D004791:Enzyme Inhibitors; D015707:HN Protein; D006603:Hexuronic Acids; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; D000080506:Sialyl Lewis X Antigen; C092488:sialic acid receptor; C036137:2-deoxy-2,3-dehydro-N-acetylneuraminic acid; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid