title: Fractionation of volatile constituents from curcuma rhizome by preparative gas chromatography.
abstract: A preparative gas chromatography (pGC) method was developed for the separation of volatile components from the methanol extract of Curcuma rhizome. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then, the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% were directed to the fraction collector. Five volatile compounds were collected from the methanol extract of Curcuma rhizome (5 g/mL) after 83 single injections (20 uL) with the yield of 5.1-46.2 mg. Furthermore, the structures of the obtained compounds were identified as β-elemene, curzerene, curzerenone, curcumenol, and curcumenone by MS and NMR spectra, respectively.
authors.affiliation: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
authors.forename: F Q
authors.initials: FQ
authors.lastname: Yang
authors.forename: H K
authors.initials: HK
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: J D
authors.initials: JD
authors.lastname: Chen
authors.forename: Z N
authors.initials: ZN
authors.lastname: Xia