title: Highly specific, sensitive and rapid enzyme immunoassays for the measurement of acetaminophen in serum.
abstract: Acetaminophen antibodies were purified using affinity chromatography and labelled with horseradish peroxidase (HRP). The antibody-HRP conjugate and a new acetaminophen derivative were used in the construction of two immunoassay methods facilitating the direct quantitative measurement of acetaminophen in serum: a 96-well microtitre plate and coated-tube ELISAs. A minimum detection limit of 0.2 μg mL(-1) and a dynamic range of 0.2 to 1 μg mL(-1) in serum were achieved using the 96-well microtitre plate ELISA. The tube assay was optimised for the measurement of the clinically critical acetaminophen concentration of 50 to 250 μg mL(-1) of serum. The quantitative and specific tests could be completed within less than an hour. Common drugs including aspirin showed less than 0.1% cross-reactivity.
authors.affiliation: Analytical Sciences Research Group, Pharmaceutical Science Research Division, School of Biomedical and Health Sciences, King's College London, University of London, 150 Stamford Street, London SE1 9NH, UK. ram.abuknesha@kcl.ac.uk
authors.forename: Ramadan A
authors.initials: RA
authors.lastname: Abuknesha
authors.forename: Michael
authors.initials: M
authors.lastname: Paleodimos
authors.forename: Fiona
authors.initials: F
authors.lastname: Jeganathan
mesh_terms: D000082:Acetaminophen / Q000097:blood* / Q000276:immunology*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G; D000082:Acetaminophen; D006735:Horseradish Peroxidase