title: Sensitive detection of endocrine disrupters using ionic liquid--single walled carbon nanotubes modified screen-printed based biosensors.
abstract: Simple and low cost biosensor based on screen-printed electrode for sensitive detection of some alkylphenols was developed, by entrapment of HRP in a nanocomposite gel based on single-walled carbon nanotubes (SWCNTs) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF(6)]) ionic liquid. Raman and FTIR spectroscopy, CV and EIS studies demonstrate the interaction between SWCNTs and ionic liquid. The nanocomposite gel, SWCNT-[BMIM][PF(6)] provides to the modified sensor a considerable enhanced electrocatalytic activity toward hydrogen peroxide reduction. The HRP based biosensor exhibits high sensitivity and good stability, allowing a detection of the alkylphenols at an applied potential of -0.2V vs. Ag/AgCl, in linear range from 5.5 to 97.7 μM for 4-t-octylphenol and respectively, between 5.5 and 140 μM for 4-n-nonylphenol, with a response time of about 5s. The detection limit was 1.1 μM for 4-t-octylphenol, and respectively 0.4 μM for 4-n-nonylphenol (S/N=3).
authors.affiliation: Laboratory of Quality Control and Process Monitoring, University of Bucharest, 4-12 Regina Elisabeta, 030018 Bucharest, Romania.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Gurban
authors.forename: Lucian
authors.initials: L
authors.lastname: Rotariu
authors.forename: Mihaela
authors.initials: M
authors.lastname: Baibarac
authors.forename: Ioan
authors.initials: I
authors.lastname: Baltog
authors.forename: Camelia
authors.initials: C
authors.lastname: Bala
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy; D004563:Electrochemistry; D004566:Electrodes; D052244:Endocrine Disruptors / Q000032:analysis* / Q000737:chemistry; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011327:Printing; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman
chemical_list: C412621:1-butyl-3-methylimidazolium hexafluorophosphate; D052244:Endocrine Disruptors; D007093:Imidazoles; D052578:Ionic Liquids; D037742:Nanotubes, Carbon; D010636:Phenols; C080417:4-octylphenol; C041594:4-nonylphenol