title: [Comparison of conventional cytogenetics and interphase fluorescence in situ hybridization in diagnosis of acute promyelocytic leukemia].
abstract: The aim of this study was to explore cytogenetic characteristics of acute promyelocytic leukemia (APL) and compare the interphase fluorescence in situ hybridization (I-FISH) with conventional cytogenetic (CC) analysis. A total number of 157 APL patients were recruited in this study, and the I-FISH and CC were applied to analyze cytogenetic features. Chromosome samples of bone marrow cells were prepared by short-term culture. Out of all 157 cases, 136 were observed with CC assay, 66 with I-FISH, of which 45 samples were analyzed with both methods. The results showed that among all 136 CC samples, t(15;17)(q22;q21) was found in 120 cases, of which 107 cases was isolated t(15;17)(q22;q21) abnormality, 13 cases was complex abnormalities and 12 case without mitotic figure. Among all 66 cases of I-FISH group, PMI/RARα fusion gene was found in 64 cases (97.0%), suggesting that I-FISH group was more sensitive than CC group (p = 0.041). It is concluded that combination of I-FISH and CC techniques plays a pivotal role for diagnosis and detection of minimal residual disease in APL.
authors.affiliation: Department of Hematology, Nanjing Medical University, Jiangsu Provincial Hospital, Nanjing, Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Kou-Rong
authors.initials: KR
authors.lastname: Miao
authors.forename: Hai-Rong
authors.initials: HR
authors.lastname: Qiu
authors.forename: Si-Xuan
authors.initials: SX
authors.lastname: Qian
authors.forename: Ming
authors.initials: M
authors.lastname: Hong
authors.forename: Chun
authors.initials: C
authors.lastname: Qiao
authors.forename: Jian-Fu
authors.initials: JF
authors.lastname: Zhag
authors.forename: Lei
authors.initials: L
authors.lastname: Fan
authors.forename: Han-Xing
authors.initials: HX
authors.lastname: Wu
authors.forename: Hua
authors.initials: H
authors.lastname: Lu
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Qiu
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Chen
authors.forename: Shu-Jiang
authors.initials: SJ
authors.lastname: Zhag
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D020732:Cytogenetic Analysis / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D007621:Karyotyping; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D055815:Young Adult