title: Metabolic adaptation of Pteris vittata L. gametophyte to arsenic induced oxidative stress.
abstract: The sporophyte and gametophyte of Pteris vittata are arsenic hyperaccumulators, however, little is known about the mechanism by which the gametophyte deals with this toxic element. An in vitro system (spores grown in arsenic amended nutrient media) was used to investigate the impact of arsenic on growth of the gametophyte and the role of antioxidative systems in combating As-stress. When mature spores of P. vittata were grown in medium amended with 0-50 mg kg(-1) of arsenic (as arsenate), the arsenic concentration in the gametophyte increased, with increasing arsenate in the media, but did not inhibit the spore germination and biomass development. Increases in the level of antioxidant enzymes, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, and glutathione-Stransferase) and of ascorbic acid and glutathione probably enabled the gametophyte to withstand the oxidative stress caused by arsenate.
authors.affiliation: CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226 001, India.
authors.forename: Anshita
authors.initials: A
authors.lastname: Raj
authors.forename: Ashutosh K
authors.initials: AK
authors.lastname: Pandey
authors.forename: Y K
authors.initials: YK
authors.lastname: Sharma
authors.forename: P B
authors.initials: PB
authors.lastname: Khare
authors.forename: Pankaj K
authors.initials: PK
authors.lastname: Srivastava
authors.forename: Nandita
authors.initials: N
authors.lastname: Singh
mesh_terms: D001151:Arsenic / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000378:metabolism; D018533:Biomass; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D032495:Pteris / Q000187:drug effects* / Q000378:metabolism; D011786:Quality Control
chemical_list: D005978:Glutathione; D001151:Arsenic; D001205:Ascorbic Acid