title: Determination of the non protein amino acid β-N-methylamino-l-alanine in estuarine cyanobacteria by capillary electrophoresis.
abstract: A capillary electrophoretic method for the determination of the amino acid β-N-methylamino-l-alanine (BMAA) was achieved using a fused-silica capillary column (50 cm × 75 μm I.D.) filled with 5 mM sodium tetraborate solution (pH 9), with an applied voltage of 25 kV, at 25 °C. The method was then applied in quantifying BMAA in eighteen strains of lyophilized estuarine cyanobacteria, following amino acid extraction using 0.1 M trichloroacetic acid and 6 M hydrochloric acid, sequentially.
authors.affiliation: LEGE, CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, R. dos Bragas 289, 4050-123 Porto, Portugal. mbaptista@ciimar.up.pt
authors.forename: Mafalda S
authors.initials: MS
authors.lastname: Baptista
authors.forename: Rosa C C
authors.initials: RC
authors.lastname: Cianca
authors.forename: Viviana R
authors.initials: VR
authors.lastname: Lopes
authors.forename: C Marisa R
authors.initials: CM
authors.lastname: Almeida
authors.forename: Vitor M
authors.initials: VM
authors.lastname: Vasconcelos
mesh_terms: D000599:Amino Acids, Diamino / Q000032:analysis*; D000458:Cyanobacteria / Q000737:chemistry*; D000087522:Cyanobacteria Toxins; D019075:Electrophoresis, Capillary / Q000379:methods*; D006868:Hydrolysis
chemical_list: D000599:Amino Acids, Diamino; D000087522:Cyanobacteria Toxins; C001824:beta-N-methylamino-L-alanine