title: [Inhibitory effect of recombinant HMGN2 protein on human hepatitis B viral].
abstract: OBJECTIVE
  To construct a prokaryotic expression recombinant for the expression of HMGN2 and to evaluate its antiviral activity against human hepatitis virus.
  
  
  METHODS
  The extracellular region cDNA of HMGN2 was isolated and amplified by RT-PCR, and introduced to the prokaryotic expression vector pGEX-4T-1. HMGN2 protein was expressed under IPTG induction and purified by GST protein purification system, then identified by SDS-PAGE and Western blot. The cytotoxicity of fusion HMGN2 to HBV-transfected HepG2. 2.15 cell was evaluated with MTT assay. Different concentration of fusion HMGN2 was applied on the HepG2. 2.15 cell and the cell culture supernatants were harvested after 3 and 6 days treatment. The HBsAg and HBeAg in the supernatants were detected by ELISA and the HBV DNA was detected by RT-PCR.
  
  
  RESULTS
  In the range of tested 1-100 microg/mL of HMGN2, no cytotoxicity to HepG2. 2.15 cells was detected by MTT assay. When incubated with HMGN2 at 15 microg/mL for 72 h or 144 h, there was a significant reduction in HBeAg and HBsAg expression as well as the HBV DNA copies.
  
  
  CONCLUSIONS
  pGEX-4T-1/HMGN2 vector was success constructed, and the recombinant HMGN2 protein could inhibit HBV expression and replication in vitro remarkably.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Xing-Li
authors.initials: XL
authors.lastname: He
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Jin
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D024242:HMGN2 Protein / Q000096:biosynthesis* / Q000235:genetics; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D024242:HMGN2 Protein; D011994:Recombinant Proteins