title: Efficacy of chitin-PAA-GTMAC gel in promoting wound healing: animal study.
abstract: Acrylic grafted chitin (chitin-PAA) was modified with glycidyltrimethylammonium chloride (GTMAC) with the aim of promoting wound healing. The chitin-PAA-GTMAC gels with different GTMAC contents were compared with the original chitin-PAA gel and Intrasite gel for their efficacy in deep wound healing of Wistar rats. Four full-thickness wounds were made on the dorsal skin of rats and then each was treated with 4 materials; chitin-PAA, chitin-PAA-GTMAC(1:4), chitin-PAA-GTMAC(1:10) and Intrasite gel. During 18 days of treatment, the wounds were visually observed and calculated for wound size using image analysis program. Skin wound tissues of sacrificed rats were processed for routine histological observation and immunohistochemistry of proliferating cell nuclear antigen (PCNA). The wounds covered with the chitin derivatives either with or without GTMAC showed a significant reduction in wound size in day 9 in comparison with day 12 for those covered with Intrasite gel. The faster rate and the better pattern of epidermal development observed in histological study as well as the higher dermal cell proliferation (PCNA expression) also demonstrated the better efficiency in wound healing of the chitin derivatives than Intrasite. The earliest epidermal development of the wounds treated with chitin-PAA-GTMAC (1:4) among the tested materials suggested the most promising of this material for the treatment of full-thickness open wound.
authors.affiliation: Faculty of Medicine Siriraj Hospital, Mahidol University, 2 Prannok Rd., Bangkok, Thailand.
authors.forename: Kajee
authors.initials: K
authors.lastname: Pilakasiri
authors.forename: Patamaporn
authors.initials: P
authors.lastname: Molee
authors.forename: Duangkamol
authors.initials: D
authors.lastname: Sringernyuang
authors.forename: Noppadon
authors.initials: N
authors.lastname: Sangjun
authors.forename: Somruethai
authors.initials: S
authors.lastname: Channasanon
authors.forename: Siriporn
authors.initials: S
authors.lastname: Tanodekaew
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials; D002686:Chitin / Q000737:chemistry / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D008422:Materials Testing; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D004852:Epoxy Compounds; D000644:Quaternary Ammonium Compounds; D002686:Chitin; C045006:glycidyl trimethylammonium; C006903:carbopol 940