title: The subcellular localization of periwinkle farnesyl diphosphate synthase provides insight into the role of peroxisome in isoprenoid biosynthesis.
abstract: Farnesyl diphosphate (FPP) synthase (FPS: EC.2.5.1.1, EC.2.5.1.10) catalyzes the formation of FPP from isopentenyl diphosphate and dimethylallyl diphosphate via two successive condensation reactions. A cDNA designated CrFPS, encoding a protein showing high similarities with trans-type short FPS isoforms, was isolated from the Madagascar periwinkle (Catharanthus roseus). This cDNA was shown to functionally complement the lethal FPS deletion mutant in the yeast Saccharomyces cerevisiae. At the subcellular level, while short FPS isoforms are usually described as cytosolic proteins, we showed, using transient transformations of C. roseus cells with yellow fluorescent protein-fused constructs, that CrFPS is targeted to peroxisomes. This finding is discussed in relation to the subcellular distribution of FPS isoforms in plants and animals and opens new perspectives towards the understanding of isoprenoid biosynthesis.
authors.affiliation: Université François-Rabelais de Tours, EA2106, Biomolécules et Biotechnologies Végétales, 37200 Tours, France.
authors.forename: Insaf
authors.initials: I
authors.lastname: Thabet
authors.forename: Grégory
authors.initials: G
authors.lastname: Guirimand
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Godet
authors.forename: Christelle
authors.initials: C
authors.lastname: Dutilleul
authors.forename: Sadok
authors.initials: S
authors.lastname: Bouzid
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Simkin
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D001692:Biological Transport; D030008:Catharanthus / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005816:Genetic Complementation Test; D051228:Geranyltranstransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045782:Hemiterpenes / Q000378:metabolism; D008164:Luminescent Proteins; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013729:Terpenes / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D045782:Hemiterpenes; D008164:Luminescent Proteins; D009943:Organophosphorus Compounds; D018749:RNA, Plant; D011993:Recombinant Fusion Proteins; D013729:Terpenes; C054562:yellow fluorescent protein, Bacteria; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D051228:Geranyltranstransferase