title: Analytical method for the simultaneous determination of polyfunctional amines used as monomers in the manufacture of food packaging materials.
abstract: This paper describes the development of a multi-analyte method for the determination of polyfunctional amines commonly used as monomers in the manufacture of food contact materials. Amines were analyzed by high-performance-liquid chromatography with diode-array detection (HPLC-DAD) after derivatization with dansyl chloride. The chromatographic analysis and the derivatization conditions were optimized. The proposed method was validated in terms of linearity, limits of detection and repeatabilities. The method showed an excellent sensitivity (LOD≤0.05 μg/mL) and appropriate repeatabilites (RSD (n=7)≤5%)). LC-MS/MS was used as a confirmatory technique. The stability of the amines in five food simulants (distilled water, 3% acetic acid, 10% ethanol, 50% ethanol and olive oil) under the most common testing conditions (10 days at 40 °C) was also studied. Results showed that amines had an acceptable stability in aqueous simulants but in the olive oil a loss of 100% was observed for all analytes.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Paseiro-Cerrato
authors.forename: A Rodríguez-Bernaldo
authors.initials: AR
authors.lastname: de Quirós
authors.forename: Raquel
authors.initials: R
authors.lastname: Sendón
authors.forename: Juana
authors.initials: J
authors.lastname: Bustos
authors.forename: E
authors.initials: E
authors.lastname: Ruíz
authors.forename: J M
authors.initials: JM
authors.lastname: Cruz
authors.forename: P
authors.initials: P
authors.lastname: Paseiro-Losada
mesh_terms: D000588:Amines / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds; D005504:Food Analysis / Q000379:methods; D018857:Food Packaging; D000432:Methanol; D008956:Models, Chemical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water
chemical_list: D000588:Amines; D003619:Dansyl Compounds; D014867:Water; C029038:dansyl chloride; D000432:Methanol