title: Permeability of endothelial barrier: cell culture and in vivo models.
abstract: The methods for assessment of endothelial barrier permeability are vital tools of experimental biology. They allow us to measure permeability of endothelial monolayer in cell culture and in lung vessels or to determine formation of tissue edema resulting from increased permeability of vasculature. This chapter provides an overview of the most common protocols.
authors.affiliation: Department of Pharmacology, University of Illinois, Chicago, IL, USA.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Garcia
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Vogel
authors.forename: Yulia A
authors.initials: YA
authors.lastname: Komarova
authors.forename: Asrar B
authors.initials: AB
authors.lastname: Malik
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D015194:Diffusion Chambers, Culture; D017097:Electric Impedance; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005070:Evans Blue / Q000032:analysis; D007365:Intercellular Junctions / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009924:Organ Culture Techniques / Q000379:methods*; D009929:Organ Size; D010539:Permeability; D011199:Potentiometry / Q000379:methods*; D011654:Pulmonary Edema / Q000378:metabolism* / Q000503:physiopathology; D012711:Serum Albumin, Radio-Iodinated / Q000032:analysis / Q000378:metabolism
chemical_list: D012711:Serum Albumin, Radio-Iodinated; D005070:Evans Blue