title: Association between genetic risk score and periodontitis onset and progression: a pilot study.
abstract: OBJECTIVE
  Recent research has focused attention on the single nucleotide polymorphisms (SNPs) involved in the host response in periodontitis. However, so as to combine the relatively small effects of individual genes the use of multi locus genetic risk (GRS) has been proposed. This study aims to evaluate whether the genetic risk score may predict periodontitis onset and progression.
  
  
  METHODS
  Fifty patients were divided into various groups according to periodontal status. Total DNA was isolated from epithelial oral cells by a masked operator and the selected SNPs were analysed. A GRS was calculated using an additive model.
  
  
  RESULTS
  We found a strong association only between TNF rs1800629 and diffused forms of periodontitis. Data show that GRS is able to discriminate diffused forms of periodontitis from localized ones. Finally, a progressive increase of the GRS is evident in advanced periodontitis in comparison with early forms.
  
  
  CONCLUSIONS
  In recent years, research on genetic polymorphism has had limited success in predicting the susceptibility to periodontal disease. However, our results indicate that the use of the genetic risk score could be promising. Further studies are necessary to include data from multiple genes so as to confirm our result.
authors.affiliation: Istituto Stomatologico Tirreno, Versilia Hospital, Camaiore (Lu), Italy. ricci.massimiliano@yahoo.it
authors.forename: M
authors.initials: M
authors.lastname: Ricci
authors.forename: F
authors.initials: F
authors.lastname: Garoia
authors.forename: C
authors.initials: C
authors.lastname: Tabarroni
authors.forename: O
authors.initials: O
authors.lastname: Marchisio
authors.forename: A
authors.initials: A
authors.lastname: Barone
authors.forename: A
authors.initials: A
authors.lastname: Genovesi
authors.forename: U
authors.initials: U
authors.lastname: Covani
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008957:Models, Genetic; D010518:Periodontitis / Q000453:epidemiology / Q000235:genetics*; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D018570:Risk Assessment; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha