title: Development and characterization of microsatellite markers in the critically endangered species Acer yangbiense (Aceraceae).
abstract: OBJECTIVE
  Microsatellite primers were developed to assess genetic diversity and population structure in Acer yangbiense, a critically endangered endemic species that occurs in northwestern Yunnan Province, China. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 34 microsatellite loci were isolated and characterized in A. yangbiense. Polymorphisms were evaluated in 39 individuals from two distinct populations, one of which was naturally occurring and the other an ex situ grouping. Nine of the markers showed polymorphisms with two to five alleles per locus; observed (H(o)) and expected (H(e)) heterozygosities ranged from 0.0000 to 0.8000 and from 0.0000 to 0.6889, respectively. •
  
  
  CONCLUSIONS
  These microsatellite primers will prove useful in the further investigation of population genetics studies in A. yangbiense and, in addition, will assist related research of other congeneric species.
authors.affiliation: Kunming Botanical Garden, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Zhao
authors.forename: Weibang
authors.initials: W
authors.lastname: Sun
authors.forename: Junbo
authors.initials: J
authors.lastname: Yang
mesh_terms: D031002:Acer / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D005819:Genetic Markers; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers