title: Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus.
abstract: Chilli ringspot virus (ChiRSV), a novel potyvirus, was recently found in Hainan, China with high prevalence. The genomic sequence of the ChiRSV-HN/14 isolate was determined by sequencing overlapping cDNA segments generated by reverse transcription polymerase chain reaction with degenerate and/or specific primers. ChiRSV genome (GenBank Acc. no. JN008909) comprised of 9,571 nucleotides (nt) excluding the 3'-terminal poly (A) tail and contained a large open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 349.1 kDa. A small, overlapping PIPO coding region was also found to span from nt 2,913 to 3,095, with a capacity to encode a peptide of 60 amino acids. ChiRSV shares sequence identities of only 48.5-65.4 and 42.9-68.7% with closely related potyviruses at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the genomic sequences provided further evidence that ChiRSV is a distinct species of the Potyvirus genus. ChiRSV-HN/14 is most closely related to Tobacco vein banding mosaic virus and two other pepper-infecting potyviruses.
authors.affiliation: Chinese Academy of Tropical Agriculture Science, Haikou, Hainan, People's Republic of China.
authors.forename: Dian
authors.initials: D
authors.lastname: Gong
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Zhan-Song
authors.initials: ZS
authors.lastname: Lin
authors.forename: Shao-Yan
authors.initials: SY
authors.lastname: Zhang
authors.forename: Yu-Liang
authors.initials: YL
authors.lastname: Zhang
authors.forename: Nai-Tong
authors.initials: NT
authors.lastname: Yu
authors.forename: Zhongguo
authors.initials: Z
authors.lastname: Xiong
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liu
mesh_terms: D001483:Base Sequence; D002212:Capsicum / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*