title: [Determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry].
abstract: A novel method for the simultaneous determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The imperatorin and isoimperatorin were extracted by methanol. The analytes were separated on a C18 column using 10 mmol/L ammonium acetate/ methanol-5 mmol/L ammonium acetate aqueous solution (80:20, v/v) as mobile phase, and then detected by HPLC-MS/MS using multiple reaction monitoring (MRM) mode. The good linearities (r > 0.999) were achieved for the target compounds over the range of 0.25-20 microg/L. The limits of quantification (LOQs) were 0.5 mg/kg. The recoveries for the imperatorin and isoimperatorin varied from 82.2% to 105% and 80.0% to 103% with the relative standard deviations ranging from 2.7% to 4.9% and 1.8% to 4.6% respectively in the range of 0.50-10.0 mg/kg. The method can meet the requirements for the simultaneous determination of imperatorin and isoimperatorin in cosmetics.
authors.affiliation: Technology Center of Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China. cjciq@yahoo.com.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiong
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D011564:Furocoumarins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003358:Cosmetics; D011564:Furocoumarins; C055542:isoimperatorin; C031534:imperatorin