title: [Distribution of hepatitis B virus genotypes and serotypes in people who had a physical examination in Yunnan Province].
abstract: OBJECTIVE
  To know genotypes and serotypes of hepatitis B virus (HBV) detected from hepatitis B infected people in Yunnan Province.
  
  
  METHODS
  Serum samples were collected from HBsAg carriers detected from people who had a physical examination at Yunnan Provincial Center for Disease Control and Prevention. The S genes of HBV were amplified by nested PCR and the PCR products were sequenced. The viral genotype was identified by phylogenetic analysis. 27 reference sequences corresponding to HBV genotype A to I were obtained from GenBank. According to the amino acid sequences deduced from the nucleotide sequences of S gene, the dominant serotype of HBV detected from these people were confirmed.
  
  
  RESULTS
  39 HBsAg positive serum samples were detected from 2216 people who had a physical examination. The results shows that 76.9% were C genotype; 15.4% were B genotype; 5.1% were D genotype; 2.5% were I genotype. Three serotypes were found. The rates of adw2, adrq+ and ayr serotypes are 71.8%, 17.9% and 10.3% respectively. All of adw2 subtype specimens are C genotype. Among the serum specimens in which both HBsAg and HBeAg are positive, 75% were C genotype and adw2 subtype.
  
  
  CONCLUSIONS
  It is determined that the main genotype and subtype of HBV prevailed in Yunnan province is C genotype and adw2 subtype.
authors.affiliation: Yunnan Provincial Center for Disease Control and Prevention, Kunming 650022, China.
authors.forename: Wen-yu
authors.initials: WY
authors.lastname: Kang
authors.forename: Zheng-rong
authors.initials: ZR
authors.lastname: Ding
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Shen
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Zhao
authors.forename: Bing-jun
authors.initials: BJ
authors.lastname: Tian
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology; D002681:China; D005260:Female; D006509:Hepatitis B / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000235:genetics / Q000276:immunology; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010808:Physical Examination; D011159:Population Surveillance
chemical_list: D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens