title: Differential acute phase immune responses by Angus and Romosinuano steers following an endotoxin challenge.
abstract: Our primary objective of this experiment was to evaluate potential genetic differences between two diverse Bos taurus breeds [Angus (AG) and Romosinuano (RO)] in response to an endotoxin challenge. Eighteen steers (n = 9 steers/breed; 299.4 ± 5.2 kg BW) were acclimated to environmentally controlled chambers maintained at thermoneutrality (19.7 °C) and then fitted with indwelling jugular catheters and rectal temperature (RT) recording devices 1 d before the endotoxin challenge. The next day, blood samples were collected at 30-min intervals from -2 to 8 h, and RT was measured continuously at 1-min intervals throughout the study. At time 0, all steers received an intravenous bolus injection of lipopolysaccharide (LPS; 2.5 μg/kg BW). Serum samples were stored at -80 °C until analyzed for cortisol, proinflammatory cytokines [tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), IL-6, and interferon gamma (IFN-γ)], and acute phase proteins (serum amyloid A, acid soluble protein, ceruloplasmin, and α-acid glycoprotein). Rectal temperatures increased in both breeds within 1 h after LPS, with RO producing a greater increase in RT than AG steers (P < 0.001). Serum cortisol and TNF-α increased (P < 0.01) in both breeds within 1 h after the LPS challenge. For cortisol, an overall breed effect (P < 0.02) was detected, such that AG steers had a higher cortisol response than RO steers. A breed × time interaction (P < 0.01) was observed for TNF-α, such that the response was delayed and extended in the RO steers compared with the AG steers. At 2 and 2.5 h after LPS, TNF-α concentrations were greater (P < 0.03) in RO steers than in AG steers. For IL-1β, a breed × time interaction (P < 0.04) was also observed. At 3 h after LPS, IL-1β concentrations were greater (P < 0.01) in RO steers than in AG steers. Serum IL-6 and IFN-γ increased (P < 0.01) in a similar manner in both groups after the LPS challenge. These data show differences in the innate immune response between two diverse Bos taurus breeds which may provide insight about differences observed in productivity, heat tolerance, disease resistance, and longevity among cattle breeds.
authors.affiliation: USDA-ARS Livestock Issues Research Unit, Lubbock, TX, USA. jeff.carroll@ars.usda.gov
authors.forename: J A
authors.initials: JA
authors.lastname: Carroll
authors.forename: N C
authors.initials: NC
authors.lastname: Burdick
authors.forename: R R
authors.initials: RR
authors.lastname: Reuter
authors.forename: C C
authors.initials: CC
authors.lastname: Chase
authors.forename: D E
authors.initials: DE
authors.lastname: Spiers
authors.forename: J D
authors.initials: JD
authors.lastname: Arthington
authors.forename: S W
authors.initials: SW
authors.lastname: Coleman
mesh_terms: D000064:Acclimatization; D000209:Acute-Phase Proteins / Q000378:metabolism; D000222:Adaptation, Physiological; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D002417:Cattle / Q000235:genetics* / Q000276:immunology* / Q000502:physiology; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006854:Hydrocortisone / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D056152:Respiratory Rate / Q000187:drug effects; D013997:Time Factors; D014329:Tropical Climate
chemical_list: D000209:Acute-Phase Proteins; D016207:Cytokines; D008070:Lipopolysaccharides; D006854:Hydrocortisone