title: Stability of plant immune-receptor resistance proteins is controlled by SKP1-CULLIN1-F-box (SCF)-mediated protein degradation.
abstract: The nucleotide-binding domain and leucine-rich repeats containing proteins (NLRs) serve as immune receptors in both plants and animals. Overaccumulation of NLRs often leads to autoimmune responses, suggesting that the levels of these immune receptors must be tightly controlled. However, the mechanism by which NLR protein levels are regulated is unknown. Here we report that the F-box protein CPR1 controls the stability of plant NLR resistance proteins. Loss-of-function mutations in CPR1 lead to higher accumulation of the NLR proteins SNC1 and RPS2, as well as autoactivation of immune responses. The autoimmune responses in cpr1 mutant plants can be largely suppressed by knocking out SNC1. Furthermore, CPR1 interacts with SNC1 and RPS2 in vivo, and overexpressing CPR1 results in reduced accumulation of SNC1 and RPS2, as well as suppression of immunity mediated by these two NLR proteins. Our data suggest that SKP1-CULLIN1-F-box (SCF) complex-mediated stability control of plant NLR proteins plays an important role in regulating their protein levels and preventing autoimmunity.
authors.affiliation: Michael Smith Laboratories, Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Yu Ti
authors.initials: YT
authors.lastname: Cheng
authors.forename: Yingzhong
authors.initials: Y
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Xinnian
authors.initials: X
authors.lastname: Dong
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D029681:Arabidopsis Proteins / Q000502:physiology*; D015551:Autoimmunity; D044783:F-Box Proteins / Q000502:physiology; D007113:Immunity, Innate; D009154:Mutation; D010641:Phenotype; D010944:Plants / Q000276:immunology* / Q000378:metabolism; D044786:S-Phase Kinase-Associated Proteins / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; C547210:CPR30 protein, Arabidopsis; D044783:F-Box Proteins; C095014:RPM1 protein, Arabidopsis; C089426:RPS2 protein, Arabidopsis; D044786:S-Phase Kinase-Associated Proteins; C482739:SNC1 protein, Arabidopsis