title: Development of an amperometric biosensor for phenol detection.
abstract: With the aim of searching for an in situ method for monitoring phenol, Agaricus bisporus tissue with tyrosine activity was used as a biocomponent and an oxygen electrode used as a transducer to develop a biosensor. The experimental methodology investigated the relation between dissolved oxygen and phenol concentration using a standard solution. Biosensor calibration was evaluated by studying reaction time and tissue amount necessary to promote a reliable response and to minimize errors. The influence of air saturation of the sample and washing of the electrode was also investigated. Results showed that 5 g of mushroom tissue with a 1 min reaction time promoted the best biosensor response within a phenol concentration range of 5-10 ppm. Washing of the electrode did not change the performance of the analysis; however, initial air saturation caused less variation amongst the samples.
authors.affiliation: Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro (UFRJ), E-122, University City, CEP 21949-909, Rio de Janeiro, Brazil.
authors.forename: L M C
authors.initials: LM
authors.lastname: Silva
authors.forename: A M
authors.initials: AM
authors.lastname: Salgado
authors.forename: M A Z
authors.initials: MA
authors.lastname: Coelho
mesh_terms: D000364:Agaricus / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D010636:Phenols; D014874:Water Pollutants, Chemical; D014442:Monophenol Monooxygenase; D010100:Oxygen