title: Direct fluorescent-antibody testing followed by culture for diagnosis of 2009 H1N1 influenza A.
abstract: During the 2009 H1N1 influenza A outbreak, 773 children were tested for influenza by direct fluorescent-antibody testing with PCR confirmation. Direct fluorescent-antibody testing has a specificity of 99.6% but a sensitivity of only 65.0%. Physicians should recognize diagnostic limitations of direct fluorescent-antibody testing, which missed one-third of infected individuals.
authors.affiliation: Phoenix Children's Hospital, Pediatric Intensive Care Unit, Phoenix, Arizona 85016, USA. pbakerm@phoenixchildrens.com
authors.forename: Paul
authors.initials: P
authors.lastname: Bakerman
authors.forename: Lilanthi
authors.initials: L
authors.lastname: Balasuriya
authors.forename: Ora
authors.initials: O
authors.lastname: Fried
authors.forename: David
authors.initials: D
authors.lastname: Tellez
authors.forename: Pamela
authors.initials: P
authors.lastname: Garcia-Filion
authors.forename: Heidi
authors.initials: H
authors.lastname: Dalton
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000737:chemistry; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral