title: Enrichment of unstable non-coding RNAs and their genome-wide identification.
abstract: Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.
authors.affiliation: Unité de Génétique des Interactions Macromoléculaires, Institut Pasteur, CNRS, URA2171, Paris, France.
authors.forename: Helen
authors.initials: H
authors.lastname: Neil
authors.forename: Alain
authors.initials: A
authors.lastname: Jacquier
mesh_terms: D001483:Base Sequence; D015925:Cryopreservation; D020869:Gene Expression Profiling; D016681:Genome, Fungal / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008969:Molecular Sequence Data; D026723:Polyadenylation; D020871:RNA Stability; D012331:RNA, Fungal / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D022661:RNA, Untranslated / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics
chemical_list: D012331:RNA, Fungal; D022661:RNA, Untranslated; D012261:Ribonucleoproteins