title: Association of A-FABP gene polymorphism in intron 1 with meat quality traits in Junmu No. 1 white swine.
abstract: This study was designed to investigate the single nucleotide polymorphism (SNP) in intron 1 of the gene A-FABP in 127 Junmu No. 1 white swine using PCR-SSCP. The association between the polymorphism and meat quality traits was also studied. The cloning and sequencing results indicated that the polymorphism of intron 1 was due to a point mutation in position 3481bp of A-FABP, giving 3 genotypes (CC, CD and DD). Association analysis indicated that the polymorphism had a significant effect on marbling (P<0.05). Genotype DD had higher marbling than CD and CC, but the difference between CD and CC was no significant. Polymorphism had a highly significant effect on intramuscular fat (IMF) content (P<0.01). DD was higher than CD, which was higher than CC. No significant conclusions can be drawn regarding other traits. Immunoblot analysis of A-FABP levels was carried out on 3 different genotype individuals. Expression was markedly reduced in DD compared with genotype CC. Thus A-FABP may be a candidate gene or a quantitative trait locus-linked gene associated with meat quality traits.
authors.affiliation: Laboratory Animal Center, Jilin University, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Yonghong Hong
authors.initials: YH
authors.lastname: Zhang
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhang
authors.forename: Fujuan
authors.initials: F
authors.lastname: Li
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Lisheng
authors.initials: L
authors.lastname: Dai
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.forename: Shuqi
authors.initials: S
authors.lastname: Xiao
authors.forename: Dianfeng
authors.initials: D
authors.lastname: Liu
authors.forename: Boxing
authors.initials: B
authors.lastname: Sun
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jiabao Bao
authors.initials: JB
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D007438:Introns / Q000235:genetics; D008297:Male; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D011786:Quality Control; D040641:Quantitative Trait Loci; D013045:Species Specificity; D013552:Swine / Q000235:genetics* / Q000502:physiology
chemical_list: D050556:Fatty Acid-Binding Proteins