title: Proteomic identification of CIB1 as a potential diagnostic factor in hepatocellular carcinoma.
abstract: Hepatocellular carcinoma (HCC), among the most common malignancies worldwide, remains a major threat to public health, and there is an urgent need to identify novel biomarkers for diagnosis, prognosis and targets for anti-cancer treatment. In this study, two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis was used to identify differentially expressed proteins among the HCC tumour centre, tumour margin and nontumourous liver tissues. In total, 52 spots with significant alteration were positively identified byMS/MSanalysis. Altered expression of representative proteins, including CIB1, was validated by Western blotting. Immunostaining suggested an increase tendency of CIB1 expression from nontumourous liver tissue to tumour centre. Knockdown of CIB1 expression by RNA interference led to the significant suppression of the cell growth in hepatoma HepG2 cells. These data suggest that CIB1 may be used as a novel prognostic factor and possibly an attractive therapeutic target for HCC.
authors.affiliation: Department of Nephrology, Guangzhou Army General Hospital Guangzhou 510010, China. tongjr63@163.com
authors.forename: Tong
authors.initials: T
authors.lastname: Junrong
authors.forename: Zhou
authors.initials: Z
authors.lastname: Huancheng
authors.forename: He
authors.initials: H
authors.lastname: Feng
authors.forename: Gao
authors.initials: G
authors.lastname: Yi
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiaoqin
authors.forename: Luo
authors.initials: L
authors.lastname: Zhengmao
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hong
authors.forename: Zeng
authors.initials: Z
authors.lastname: Jianying
authors.forename: Wang
authors.initials: W
authors.lastname: Yin
authors.forename: Huang
authors.initials: H
authors.lastname: Yuanhang
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jianlin
authors.forename: Sun
authors.initials: S
authors.lastname: Longhua
authors.forename: He
authors.initials: H
authors.lastname: Guolin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D020868:Gene Silencing / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D040901:Proteomics / Q000379:methods; D034741:RNA, Small Interfering / Q000494:pharmacology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D016019:Survival Analysis; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C109198:CIB1 protein, human; D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering