title: Association of ADAM33 gene polymorphisms with adult-onset asthma and its severity in an Indian adult population.
abstract: ADAM33, a member of the ADAM (a disintegrin and metalloprotease) gene family, is an asthma susceptibility gene originally identified by positional cloning. In the present study, we investigated the possible association of five single-nucleotide polymorphisms (SNPs) in the ADAM33 (rs511898, rs528557, rs44707, rs597980 and rs2787094) with adult-onset asthma in an Indian population. The study included 175 patients with mild intermittent (n = 44), mild persistent (n = 108) or moderate persistent (n = 23) subgroups of asthma, and 253 nonasthmatic control individuals. SNPs were genotyped with the help of restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) method, and data were analysed using chi-square test and logistic regression model. Bonferroni's correction for multiple comparisons was applied for each hypothesis. Genotypes and allele frequencies of SNPs rs511898 and rs528557 were significantly associated with adult-onset asthma (P = 0.010-<0.001). A significant association of the homozygous mutant genotype and mutant alleles of SNPs rs2787094, rs44707 and rs597980 with the asthma was also observed (P = 0.020-<0.001). A positive association between asthma and haplotypes AGCCT, GGCCT, AGACT, GCAGT, GGACT, ACCCC and AGACC were also found (P = 0.036-<0.001, OR = 2.07-8.49). Haplotypes AGCGT, GCAGC, ACAGC, ACAGT, GGAGC and GGCGT appear to protect against asthma (P = 0.013-<0.0001, OR = 0.34-0.10). Our data suggest that ADAM33 gene polymorphisms serve as genetic risk factors for asthma in Indian adult population.
authors.affiliation: Department of Pediatrics, Chhtrapati Shahuji Maharaj Medical University, Chowk, Lucknow 226 003, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Tripathi
authors.forename: Shally
authors.initials: S
authors.lastname: Awasthi
authors.forename: Rajendra
authors.initials: R
authors.lastname: Prasad
authors.forename: Nuzhat
authors.initials: N
authors.lastname: Husain
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Ganesh
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000328:Adult; D001249:Asthma / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D007194:India; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D055815:Young Adult
chemical_list: D051722:ADAM Proteins; C450441:ADAM33 protein, human