title: Vascular and micro-environmental influences on MSC-coral hydroxyapatite construct-based bone tissue engineering.
abstract: Bone tissue engineering (BTE) has been demonstrated an effective approach to generate bone tissue and repair bone defect in ectopic and orthotopic sites. The strategy of using a prevascularized tissue-engineered bone grafts (TEBG) fabricated ectopically to repair bone defects, which is called live bone graft surgery, has not been reported. And the quantitative advantages of vascularization and osteogenic environment in promoting engineered bone formation have not been defined yet. In the current study we generated a tissue engineered bone flap with a vascular pedicle of saphenous arteriovenous in which an organized vascular network was observed after 4 weeks implantation, and followed by a successful repaire of fibular defect in beagle dogs. Besides, after a 9 months long term observation of engineered bone formation in ectopic and orthotopic sites, four CHA (coral hydroxyapatite) scaffold groups were evaluated by CT (computed tomography) analysis. By the comparison of bone formation and scaffold degradation between different groups, the influences of vascularization and micro-environment on tissue engineered bone were quantitatively analyzed. The results showed that in the first 3 months vascularization improved engineered bone formation by 2 times of non-vascular group and bone defect micro-environment improved it by 3 times of ectopic group, and the CHA-scaffold degradation was accelerated as well.
authors.affiliation: Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100144, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Congmin
authors.initials: C
authors.lastname: Gu
authors.forename: Jingguo
authors.initials: J
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Kang
authors.forename: Jiewei
authors.initials: J
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Yan
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
authors.forename: Ran
authors.initials: R
authors.lastname: Xiao
mesh_terms: D000792:Angiography; D000818:Animals; D037421:Anthozoa; D001842:Bone and Bones / Q000098:blood supply / Q000166:cytology*; D002454:Cell Differentiation; D004285:Dogs; D006882:Hydroxyapatites; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D014057:Tomography, X-Ray Computed
chemical_list: D006882:Hydroxyapatites