title: Development and validation of a liquid chromatography-tandem mass spectrometry method for the identification and quantification of JWH-018, JWH-073, JWH-019, and JWH-250 in human whole blood.
abstract: A sensitive and specific method for the quantification of JWH-018, JWH-073, and JWH-250 and the qualitative identification of JWH-019 in whole blood was developed and validated. Samples fortified with JWH-018-d₉ and JWH-073-d₉ underwent liquid-liquid extraction and were analyzed by liquid chromatography-positive ion electrospray ionization-tandem mass spectrometry. Two transitions were monitored for all analytes except JWH-250, for which there was only one available transition. JWH-019 did not meet the stringent requirements for quantitative analysis, and thus this method is only appropriate for the qualitative identification of this compound in whole blood. The linear range was 0.1-20 μg/L for all quantitative analytes. The maximum average within and between-run imprecision was 7.9% and 10.2%, respectively, and all controls quantified within 8.2% of target concentrations. Process efficiency, a measurement that takes into effect extraction efficiency and matrix effect, was ≥ 32.0% for all quantitative analytes; similar results were obtained for the deuterated internal standards. All analytes were stable at room, refrigerated, and frozen temperatures for at least 30 days. The method was used to quantify JWH-018 and JWH-073 in a blood specimen collected from a person known to have used an herbal incense blend containing these substances.
authors.affiliation: NMS Labs, 3701 Welsh Rd., Willow Grove, Pennsylvania 18901, USA.
authors.forename: Sherri L
authors.initials: SL
authors.lastname: Kacinko
authors.forename: Allan
authors.initials: A
authors.lastname: Xu
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Homan
authors.forename: Matthew M
authors.initials: MM
authors.lastname: McMullin
authors.forename: Donna M
authors.initials: DM
authors.lastname: Warrington
authors.forename: Barry K
authors.initials: BK
authors.lastname: Logan
mesh_terms: D000840:Anisoles / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000097:blood*; D007211:Indoles / Q000097:blood*; D009281:Naphthalenes / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: C569193:(1-hexyl-1H-indol-3-yl)-1-naphthalenylmethanone; C569194:1-(1- pentyl-1H-indol-3-yl)-2-(2-methoxyphenyl)ethanone; D000840:Anisoles; D013287:Illicit Drugs; D007211:Indoles; D009281:Naphthalenes; C568258:JWH-073; C552597:1-pentyl-3-(1-naphthoyl)indole