title: Analysis of bulk and virus-specific CD8+ T cells reveals advanced differentiation of CD8+ T cells in patients with common variable immunodeficiency.
abstract: Common variable immunodeficiency (CVID) is a heterogeneous antibody deficiency syndrome with alterations in T cell regulation and function in a subgroup of patients. We assessed phenotype and function of bulk and virus-specific CD8+ T cells of a cohort of 34 HLA-A2+ CVID patients by pentamer technology and flow cytometry in relationship to their immunological and clinical phenotypes. Bulk CD8+ T cells displayed a shift toward a more antigen experienced and activated differentiation state. The advanced differentiation pattern was mainly found in a subgroup of CVID patients with lymphadenopathy and granulomatous disease. This effect existed independently of the patients' CMV status even so CMV-associated immunosenescence was more evident in CVID patients than in CMV-positive immunocompetent controls. As the phenotype and function of virus-specific CD8+ T cells were normal in CVID the induction of antiviral immunity by prophylactic immunization appears to be a logical and desirable aim for this group of patients.
authors.affiliation: Department of Medicine II, University Medical Center Freiburg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Kuntz
authors.forename: Sigune
authors.initials: S
authors.lastname: Goldacker
authors.forename: Hubert E
authors.initials: HE
authors.lastname: Blum
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Pircher
authors.forename: Susanne
authors.initials: S
authors.lastname: Stampf
authors.forename: Hans-Hartmut
authors.initials: HH
authors.lastname: Peter
authors.forename: Robert
authors.initials: R
authors.lastname: Thimme
authors.forename: Klaus
authors.initials: K
authors.lastname: Warnatz
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D017074:Common Variable Immunodeficiency / Q000276:immunology* / Q000473:pathology; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology; D020031:Epstein-Barr Virus Infections / Q000097:blood / Q000276:immunology; D005260:Female; D006099:Granuloma / Q000209:etiology / Q000276:immunology; D004854:Herpesvirus 4, Human / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D016130:Immunophenotyping; D008206:Lymphatic Diseases / Q000209:etiology / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009842:Oligopeptides / Q000276:immunology; D009975:Orthomyxoviridae / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D010750:Phosphoproteins / Q000276:immunology; D037182:T-Cell Antigen Receptor Specificity; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology; D014766:Viremia / Q000276:immunology; D055815:Young Adult
chemical_list: D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; D009842:Oligopeptides; D010446:Peptide Fragments; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa; C403195:glycyl-leucyl-cysteinyl-threonyl-leucyl-valyl-alanyl-methionyl-leucine; C448634:influenza matrix peptide (58-66)