title: Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats.
abstract: The present study investigated the effects of resveratrol (RV), a polyphenol with potent antioxidant properties, on oxidative stress parameters in liver and kidney, as well as on serum biochemical parameters of streptozotocin (STZ)-induced diabetic rats. Animals were divided into six groups (n = 8): control/saline; control/RV 10 mg/kg; control/RV 20 mg/kg; diabetic/saline; diabetic/RV10 mg/kg; diabetic/RV 20 mg/kg. After 30 days of treatment with resveratrol the animals were sacrificed and the liver, kidney and serum were used for experimental determinations. Results showed that TBARS levels were significantly increased in the diabetic/saline group and the administration of resveratrol prevented this increase in the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). The activities of catalase (CAT), superoxide dismutase (SOD) and aminolevulinic acid dehydratase (δ-ALA-D) and the levels of non protein thiols (NPSH) and vitamin C presented a significant decrease in the diabetic/saline group when compared with the control/saline group (P < 0.05). The treatment with resveratrol was able to prevent these decrease improving the antioxidant defense of the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). In addition, the elevation in serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamiltransferase (γ-GT) activities as well as in levels of urea, creatinine, cholesterol and triglycerides observed in the diabetic/saline group were reverted to levels close to normal by the administration of resveratrol in the diabetic/RV10 and diabetic/RV20 groups (P < 0.05). These findings suggest that resveratrol could have a protector effect against hepatic and renal damage induced by oxidative stress in the diabetic state, which was evidenced by the capacity of this polyphenol to modulate the antioxidant defense and to decrease the lipid peroxidation in these tissues.
authors.affiliation: Programa de Pós Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil. betaschmatz@hotmail.com
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Luciane Belmonte
authors.initials: LB
authors.lastname: Perreira
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Cinthia
authors.initials: C
authors.lastname: Mazzanti
authors.forename: Roselia
authors.initials: R
authors.lastname: Spanevello
authors.forename: Jessié
authors.initials: J
authors.lastname: Gutierres
authors.forename: Margarete
authors.initials: M
authors.lastname: Bagatini
authors.forename: Caroline Curry
authors.initials: CC
authors.lastname: Martins
authors.forename: Fátima Husein
authors.initials: FH
authors.lastname: Abdalla
authors.forename: Jonas
authors.initials: J
authors.lastname: Daci da Silva Serres
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Juliano Marchi
authors.initials: JM
authors.lastname: Vieira
authors.forename: Andréia Machado
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Morsch
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000627:therapeutic use; D013311:Streptozocin / Q000009:adverse effects; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D013267:Stilbenes; D017392:Thiobarbituric Acid Reactive Substances; D013311:Streptozocin; D002374:Catalase; D013482:Superoxide Dismutase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000623:Porphobilinogen Synthase; D000077185:Resveratrol