title: Enhanced isolation of Legionella species from composted material.
abstract: Legionella pneumophila and Legionella species were isolated from composted material when freshly prepared buffered charcoal yeast extract (BCYE) was supplemented with glycine (1.5 g/L), polymyxin B sulfate (40 000 IU/L), vancomycin hydrochloride (0.5 mg/L) and cycloheximide (40 mg/L) (GVPC medium) and Modified Wadowsky-Yee (MWY) (Oxoid, Cambridge, UK) plates were used for cultivation, but not with commercially sourced pre-poured GVPC and MWY plates (Oxoid). Legionella cincinnatiensis and pathogenic L. pneumophila serogroup (Sg) 1 Benidorm and France/Allentown were identified, as well as a non-typeable (NT) strain of L. pneumophila. As most laboratories no longer produce their own media, this may contribute to the lack of positive cultures from composted material. The antigenicity of the NT strain is discussed.
authors.affiliation: Scottish Haemophilus Legionella Meningococcus Pneumococcus Reference Laboratory (SHLMPRL), House on the Hill, Stobhill Hospital, Glasgow, UK.
authors.forename: S
authors.initials: S
authors.lastname: McCabe
authors.forename: A
authors.initials: A
authors.lastname: Brown
authors.forename: G F S
authors.initials: GF
authors.lastname: Edwards
authors.forename: D
authors.initials: D
authors.lastname: Lindsay
mesh_terms: D000942:Antigens, Bacterial / Q000276:immunology; D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry*; D003513:Cycloheximide / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D007124:Immunoenzyme Techniques; D018189:Immunomagnetic Separation; D007875:Legionella / Q000145:classification / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D011112:Polymyxin B / Q000737:chemistry; D012987:Soil / Q000032:analysis / Q000737:chemistry*; D012988:Soil Microbiology; D014640:Vancomycin / Q000737:chemistry
chemical_list: D000942:Antigens, Bacterial; D003470:Culture Media; D012987:Soil; D014640:Vancomycin; D003513:Cycloheximide; D011112:Polymyxin B; D005998:Glycine