title: Immunohistochemical detection of HSP27 and hnRNP K as prognostic and predictive biomarkers for colorectal cancer.
abstract: The present study was aimed at evaluating the expression of heat shock protein 27 (HSP27) and heterogeneous nuclear ribonucleoprotein K (hnRNP K), two potential biomarkers of many cancers, in colorectal cancer (CRC) and their clinical significance. Expression of HSP27 and hnRNP K were investigated by immunohistochemistry (IHC) in a series of tissue microarrays containing 175 primary colorectal cancers and their corresponding normal mucosa samples and matched with clinicopathological features and patient survival. HSP27 and hnRNP K displayed more frequent strong immunoreactivity in primary colorectal tumor samples compared with adjacent non-cancer tissue (P < 0.001). Increased cytoplasmic expression of HSP27 and hnRNP K were associated with tumor location (P = 0.032 and P < 0.001, respectively), poorer overall survival (P = 0.004 and P = 0.02, respectively) and to an unfavorable prognosis for CRC patients in multivariate analysis (P = 0.019 and P = 0.01, respectively). Their overexpression combination identified a subset of patients with definitively worse prognosis than any other combination (P < 0.001). Overexpression of HSP27 and hnRNPK were independent markers of poor prognosis, and their combination predicted definitively adverse outcomes in CRC patients.
authors.affiliation: Department of Surgery, The Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Chenzhang
authors.initials: C
authors.lastname: Shi
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Yang
authors.forename: Huanlong
authors.initials: H
authors.lastname: Qin
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D034641:Heterogeneous-Nuclear Ribonucleoprotein K / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D016016:Proportional Hazards Models; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; D034641:Heterogeneous-Nuclear Ribonucleoprotein K