title: CD8α(+) dendritic cells are an obligate cellular entry point for productive infection by Listeria monocytogenes.
abstract: CD8α(+) dendritic cells (DCs) prime cytotoxic T lymphocytes during viral infections and produce interleukin-12 in response to pathogens. Although the loss of CD8α(+) DCs in Batf3(-/-) mice increases their susceptibility to several pathogens, we observed that Batf3(-/-) mice exhibited enhanced resistance to the intracellular bacterium Listeria monocytogenes. In wild-type mice, Listeria organisms, initially located in the splenic marginal zone, migrated to the periarteriolar lymphoid sheath (PALS) where they grew exponentially and induced widespread lymphocyte apoptosis. In Batf3(-/-) mice, however, Listeria organisms remain trapped in the marginal zone, failed to traffic into the PALS, and were rapidly cleared by phagocytes. In addition, Batf3(-/-) mice, which lacked the normal population of hepatic CD103(+) peripheral DCs, also showed protection from liver infection. These results suggest that Batf3-dependent CD8α(+) and CD103(+) DCs provide initial cellular entry points within the reticuloendothelial system by which Listeria establishes productive infection.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
authors.forename: Brian T
authors.initials: BT
authors.lastname: Edelson
authors.forename: Tara R
authors.initials: TR
authors.lastname: Bradstreet
authors.forename: Kai
authors.initials: K
authors.lastname: Hildner
authors.forename: Javier A
authors.initials: JA
authors.lastname: Carrero
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Frederick
authors.forename: Wumesh
authors.initials: W
authors.lastname: KC
authors.forename: Roger
authors.initials: R
authors.lastname: Belizaire
authors.forename: Taiki
authors.initials: T
authors.lastname: Aoshi
authors.forename: Robert D
authors.initials: RD
authors.lastname: Schreiber
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Miller
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Murphy
authors.forename: Emil R
authors.initials: ER
authors.lastname: Unanue
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Murphy
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D017209:Apoptosis / Q000235:genetics; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics; D016827:CD8 Antigens / Q000096:biosynthesis; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D007113:Immunity, Innate / Q000235:genetics; D039001:Integrin alpha Chains / Q000096:biosynthesis; D008089:Listeria monocytogenes / Q000472:pathogenicity / Q000502:physiology*; D008088:Listeriosis / Q000276:immunology* / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D010587:Phagocytosis / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D013154:Spleen / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D014774:Virulence
chemical_list: D015703:Antigens, CD; D050976:Basic-Leucine Zipper Transcription Factors; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D039001:Integrin alpha Chains; D012097:Repressor Proteins; C533289:SNFT protein, mouse; C091367:alpha E integrins