title: The effect of current used bone substitution materials and platelet-rich plasma on periosteal cells by ectopic site implantation: an in-vivo pilot study.
abstract: The aim of this study was to investigate de novo bone formation following ectopic site implantation of bone substitutes covered by periosteum, with and without the application of autologous platelet-rich plasma (PRP). Twenty-four weeks after subcutaneous implantation of various bone substitutes (bovine hydroxyapatite (bHAP), phycogenic hydroxyapatite (pHAP), and bioglass (BG)) in 35 mini-pigs, bone regeneration rates were compared microradiographically and histologically. Without PRP, bHAP showed a mean de novo bone formation of 32.41%±29.99, in contrast to the other substitute materials where no mineralization could be detected. In combination with PRP, in the bHAP (63.61%±12.98; p±0.03) and pHAP (34.37±29.38; p=0.015) group, significantly higher de novo bone formation was ascertained than without PRP. No ossification could be detected in the BG group. In conclusion, bHAP and pHAP bone substitutes in combination with PRP showed a significant positive effect on periosteal cells by de novo bone formation after ectopic, subcutaneous, low-vascular site implantation.
authors.affiliation: Department of Craniomaxillofacial and Oral Surgery (Head: Klaus Wilhelm Grätz, MD, DMD, PhD), University of Zurich, Frauenklinikstrasse 24, 8091 Zurich, Switzerland. philipp.metzler@usz.ch
authors.forename: Philipp
authors.initials: P
authors.lastname: Metzler
authors.forename: Cornelius
authors.initials: C
authors.lastname: von Wilmowsky
authors.forename: Robert
authors.initials: R
authors.lastname: Zimmermann
authors.forename: Jörg
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Karl Andreas
authors.initials: KA
authors.lastname: Schlegel
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use*; D002113:Calcification, Physiologic / Q000187:drug effects; D002417:Cattle; D002516:Ceramics; D017886:Durapatite / Q000627:therapeutic use; D005898:Glass; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008852:Microradiography; D008903:Minerals / Q000627:therapeutic use; D010012:Osteogenesis / Q000187:drug effects*; D010521:Periosteum / Q000166:cytology / Q000187:drug effects*; D010865:Pilot Projects; D053657:Platelet-Rich Plasma / Q000502:physiology*; D050151:Subcutaneous Fat / Q000473:pathology / Q000601:surgery; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: C483718:Algipore; C077540:Bio-Oss; C022107:Bioglass; C073544:Biogran; D018786:Bone Substitutes; D008903:Minerals; D017886:Durapatite