title: Light and moderate doses of ethanol in chemical carcinogenesis of the colon in rats.
abstract: The aberrant crypt foci (ACF), cyclooxygenase 2 (COX-2), and the proliferating cell nuclear antigen (PCNA) are putative biomarkers for colon cancer. To study the association between light (1 g of ethanol/kg bw) and moderate (3 g of ethanol/kg bw) doses of ethanol with the chemical carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), Wistar rats were divided into 6 groups. The colon fragments were collected for histochemical and immunohistochemical analyses, and the liver samples were collected for oxidative stress analysis, with products of lipid peroxidation (malondialdehyde), antioxidant enzymes (glutathione), and vitamin E. The association of light and moderate doses of ethanol with MNNG did not present differences in the oxidative parameters. However, a reduction in vitamin E levels in the carcinogen groups was observed. The association induced a reduction of the COX-2 and PCNA expression. The number of ACF in the group that received a light dose of ethanol had lower rates, while the group that received a moderate dose had the highest rates compared to the control MNNG, demonstrating that the light dose of ethanol could have a protective effect, while the moderate dose could represent a risk during chemical carcinogenesis in rats.
authors.affiliation: Department of Pathology and Legal Medicine, Cell Proliferation Laboratory, Medicine School of Ribeirão Preto, University of São Paulo, Brazil. jfelipe.vet@gmail.com
authors.forename: João Felipe Rito
authors.initials: JF
authors.lastname: Cardoso
authors.forename: Célia
authors.initials: C
authors.lastname: Cohen
authors.forename: Alceu Afonso
authors.initials: AA
authors.lastname: Jordão
authors.forename: Hélio
authors.initials: H
authors.lastname: Vannucchi
authors.forename: Sérgio Britto
authors.initials: SB
authors.lastname: Garcia
authors.forename: Sérgio
authors.initials: S
authors.lastname: Zucoloto
mesh_terms: D058739:Aberrant Crypt Foci / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002273:Carcinogens / Q000633:toxicity*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage* / Q000633:toxicity*; D005978:Glutathione / Q000032:analysis; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D018384:Oxidative Stress; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000032:analysis
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002273:Carcinogens; D018809:Proliferating Cell Nuclear Antigen; D008769:Methylnitronitrosoguanidine; D014810:Vitamin E; D000431:Ethanol; D008315:Malondialdehyde; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D005978:Glutathione