title: Patterning small-molecule biocapture surfaces: microcontact insertion printing vs. photolithography.
abstract: Chemical patterns prepared by self-assembly, combined with soft lithography or photolithography, are directly compared. Pattern fidelity can be controlled in both cases but patterning at the low densities necessary for small-molecule probe capture of large biomolecule targets is better accomplished using microcontact insertion printing (μCIP). Surfaces patterned by μCIP are used to capture biomolecule binding partners for the small molecules dopamine and biotin.
authors.affiliation: Center for Nanoscale Science, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Shuster
authors.forename: A
authors.initials: A
authors.lastname: Vaish
authors.forename: H H
authors.initials: HH
authors.lastname: Cao
authors.forename: A I
authors.initials: AI
authors.lastname: Guttentag
authors.forename: J E
authors.initials: JE
authors.lastname: McManigle
authors.forename: A L
authors.initials: AL
authors.lastname: Gibb
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Rivera
authors.forename: M M
authors.initials: MM
authors.lastname: Martinez
authors.forename: R M
authors.initials: RM
authors.lastname: Nezarati
authors.forename: J M
authors.initials: JM
authors.lastname: Hinds
authors.forename: W-S
authors.initials: WS
authors.lastname: Liao
authors.forename: P S
authors.initials: PS
authors.lastname: Weiss
authors.forename: A M
authors.initials: AM
authors.lastname: Andrews
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001710:Biotin / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006834:Hydrazines / Q000737:chemistry; D008856:Microscopy, Fluorescence; D011817:Rabbits; D019809:Streptavidin / Q000276:immunology; D013499:Surface Properties
chemical_list: C417561:Alexa 488 hydrazide; D000906:Antibodies; D006834:Hydrazines; D001710:Biotin; D006046:Gold; D019809:Streptavidin; D004298:Dopamine