title: Dielectrophoresis-assisted massively parallel cell pairing and fusion based on field constriction created by a micro-orifice array sheet.
abstract: In this paper, we present a novel electrofusion device that enables massive parallelism, using an electrically insulating sheet having a two-dimensional micro-orifice array. The sheet is sandwiched by a pair of micro-chambers with immersed electrodes, and each chamber is filled with the suspensions of the two types of cells to be fused. Dielectrophoresis, assisted by sedimentation, is used to position the cells in the upper chamber down onto the orifices, then the device is flipped over to position the cells on the other side, so that cell pairs making contact in the orifice are formed. When a pulse voltage is applied to the electrodes, most voltage drop occurs around the orifice and impressed on the cell membrane in the orifice. This makes possible the application of size-independent voltage to fuse two cells in contact at all orifices exclusively in 1:1 manner. In the experiment, cytoplasm of one of the cells is stained with a fluorescence dye, and the transfer of the fluorescence to the other cell is used as the indication of fusion events. The two-dimensional orifice arrangement at the pitch of 50 μm realizes simultaneous fusion of 6 × 10³ cells on a 4 mm diameter chip, and the fusion yield of 78-90% is achieved for various sizes and types of cells.
authors.affiliation: Department of Bioengineering, the University of Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kimura
authors.forename: Murat
authors.initials: M
authors.lastname: Gel
authors.forename: Boonchai
authors.initials: B
authors.lastname: Techaumnat
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Oana
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kotera
authors.forename: Masao
authors.initials: M
authors.lastname: Washizu
mesh_terms: D000818:Animals; D002459:Cell Fusion / Q000295:instrumentation* / Q000379:methods*; D045744:Cell Line, Tumor; D004566:Electrodes; D004586:Electrophoresis / Q000295:instrumentation*; D004867:Equipment Design; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D010316:Particle Size; D046888:Tissue Array Analysis / Q000295:instrumentation*
chemical_list: D005452:Fluoresceins