title: Serologic survey of the pandemic H1N1 2009 virus in Guangdong Province, China: a cross sectional study.
abstract: BACKGROUND
  Relying on surveillance of clinical cases limits the ability to understand the full impact and severity of an epidemic, which urges a deep insight into the serological evidence of infection and transmission feature of pandemic H1N1 2009 (pH1N1) virus in Guangdong province.
  
  
  METHODS
  In this cross-sectional serological survey, serum samples were collected by multi-stage stratified random sampling in Jan 2010. Antibody titers were measured by hemagglutination inhibition (HI) assay. Age-specific and region-specific prevalence were calculated based on the results of HI assay (positive, HI titer≥1:40).
  
  
  RESULTS
  A total of 4,319 serum samples had been collected from subjects without vaccination with pH1N1 vaccine. The seroprevalence was 22.82% (985/4,319). By contrast, there was a marked spatial heterogeneity in prevalence. The seroprevalence was 27.3% in large city, 21.4% in medium cities, higher than that of 20.2% in rural areas. The seroprevalence was highest in 11-20 age group (32.8%), however, in those above 60 years of age group, which was 12.6%, lower than other age groups. On the other hand, antibody titers to pH1N1 virus were highest in school children, which were followed by a gradual decrease in adult. However, in the elderly groups from cities, especially from large city, the antibody titer to pH1N1 increased significantly and reached a much higher level.
  
  
  CONCLUSIONS
  Our results showed that the prevalence for pH1N1 was correlated with age and population density. Preexisting antibody may have protected the very old from pH1N1 infection, while original antigenic sin and immunosenescence may have contributed to greater severity once infected. These should be considered when studying the pathogenesis and transmission of influenza virus and formulating strategies on vaccination and treatment.
authors.affiliation: Center for Disease Control and Prevention of Guangdong Province, Guangzhou, People's Republic of China. zhangxinhui16@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianfeng
authors.initials: J
authors.lastname: He
authors.forename: Linghui
authors.initials: L
authors.lastname: Li
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhu
authors.forename: Changwen
authors.initials: C
authors.lastname: Ke
authors.forename: Hanzhong
authors.initials: H
authors.lastname: Ni
authors.forename: Nianmei
authors.initials: N
authors.lastname: Hou
authors.forename: Haojie
authors.initials: H
authors.lastname: Zhong
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003430:Cross-Sectional Studies; D005260:Female; D005843:Geography; D019538:Health Care Surveys / Q000706:statistics & numerical data*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D058873:Pandemics / Q000706:statistics & numerical data*; D012698:Serologic Tests / Q000379:methods*; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral