title: [Cyclin D1, hTERT expression and telomerase activity in HL-60 and HL-60A cell lines and their significance].
abstract: To observe the expression of cyclin D1, hTERT, and telomerase activity in MNC, HL-60, HL-60A and to explore their effects on leukemogenesis and drug-resistance, normal human peripheral blood mononuclear cells, HL-60 cells sensitive to adriamycin and HL-60A cells resistant to adriamycin were investigated. The cell cycle was analyzed by flow cytometry, and the apoptosis was analyzed by Annexin V-FITC(+) PI staining. Expressions of cyclin D1 and hTERT were determined by real-time PCR and Western blot. Telomerase activity was detected by TRAP-ELISA. The results indicated that the percentage of MNC, HL-60 and HL-60A in S phase was (10.21 + 2.11)%, (44.93 + 3.00)%, and (51.38 + 1.10)% respectively; the percentage of apoptosis cells was (16.14 + 2.13)%, (7.53 + 0.92)%, (4.15 + 0.96)% respectively; the expression of mRNA and protein for cyclin D1 and hTERT increased; the telomerase activities of HL-60 and HL-60A were higher (p = 0.000), whereas the difference between HL-60 and HL-60A was no statistically significant (p = 0.232); positive correlation between cyclin D1, hTERT and telomerase activity had been found (p < 0.01). It is concluded that the cells of S phase increased while the apoptotic cells decreased in HL-60 and HL-60A, especially in HL-60A, which may be due to the up-regulation of cyclin D1, hTERT and telomerase activity.
authors.affiliation: Department of Hematology, Sun Yat-Sen University, Guangzhou, Guangdong Province, China.
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Huang
authors.forename: Da-Nian
authors.initials: DN
authors.lastname: Nie
authors.forename: Song-Mei
authors.initials: SM
authors.lastname: Yin
authors.forename: Yi-Qing
authors.initials: YQ
authors.lastname: Li
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Xie
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ma
authors.forename: Xiu-Ju
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yu-Dan
authors.initials: YD
authors.lastname: Wu
mesh_terms: D002453:Cell Cycle; D019938:Cyclin D1 / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D019098:Telomerase / Q000378:metabolism*
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1; C509186:TERT protein, human; D019098:Telomerase