title: Epidermal permeability barrier measurement in mammalian skin.
abstract: A defective skin epidermal permeability barrier (EPB) is responsible for a high mortality rate in premature infants and is an important risk factor in inflammatory skin diseases such as eczema. We report here fast and accurate methods for measurement of EPB in animal models or in human patients using simple techniques that monitor diffusion of dyes (X-Gal or Lucifer Yellow) through the upper epidermis and measure transepidermal water loss (TEWL) resulting from a defective skin barrier. Accurate diagnosis and early detection of EPB defects in human patients are critical for effective treatment of certain classes of inflammatory skin diseases.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, and Environmental Health Sciences Center, Oregon State University, Corvallis, OR, USA. Arup.Indra@oregonstate.edu
authors.forename: Arup Kumar
authors.initials: AK
authors.lastname: Indra
authors.forename: Mark
authors.initials: M
authors.lastname: Leid
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D004058:Diffusion; D004485:Eczema / Q000378:metabolism / Q000473:pathology; D004622:Embryo, Mammalian / Q000378:metabolism*; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D005697:Galactosides / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000032:analysis* / Q000378:metabolism; D007223:Infant; D007546:Isoquinolines / Q000032:analysis* / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008322:Mammals; D051379:Mice; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D010539:Permeability; D011247:Pregnancy; D012097:Repressor Proteins / Q000172:deficiency* / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000172:deficiency* / Q000235:genetics; D014870:Water Loss, Insensible
chemical_list: C511143:Bcl11b protein, mouse; D005456:Fluorescent Dyes; D005697:Galactosides; D007211:Indoles; D007546:Isoquinolines; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; C017475:lucifer yellow; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside