title: Effects of intra-abdominal pressure increase on intestinal ischemia and bacterial translocation in experimental sepsis model.
abstract: OBJECTIVE
  To investigate the safety of laparoscopic intervention for diagnosis and treatment at 8 mm Hg pressure in one-hour period on acute peritonitis related intra-abdominal sepsis model.
  
  
  METHODS
  In this study, we included 32 female Wistar-Albino rats, weighing 250 +/- 20 g, and divided them into 4 groups. This study was conducted in Istanbul University Experimental Medical Research Institution (DETAE) laboratory from April to May 2009. Intra-abdominal sepsis was created with intraperitoneal (i.p.) one mL (109 CFU/mL) Escherichia coli (E. coli) injection, and pneumoperitoneum was formed with CO2 insufflation at 8 mm Hg pressure for one hour i.p. The rats were administered with: Group 1 - one mL i.p. isotonic saline; Group 2 - one mL i.p. isotonic saline + pneumoperitoneum; Group 3 - i.p E. coli; and Group 4 - i.p. E.coli + pneumoperitoneum. Data were analyzed using the Statistical Package for Social Sciences version 15 for Windows (SPSS Inc, Chicago, IL, USA).
  
  
  RESULTS
  Fever and leukocyte values were considered high in Groups 3 and 4 compared with Groups 1 and 2 (p=0.001). The administered reproduction ratio of the E. coli strain was determined as 0% in Groups 1 and 2, and 100% in Groups 3 and 4.
  
  
  CONCLUSIONS
  In this study, as pneumoperitoneum was formed for one hour at 8 mm Hg pressure, in case of intra-abdominal derived sepsis where emergency intervention is needed, we consider that laparoscopic approaches with low pressure may be used safely for diagnosis and treatment.
authors.affiliation: Department of General Surgery, Akcaabat State Hospital, Ministry of Health, Trabzon, Turkey. ugurkesici77@mynet.com
authors.forename: Ugur
authors.initials: U
authors.lastname: Kesici
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kesici
authors.forename: Erdal
authors.initials: E
authors.lastname: Polat
authors.forename: Birol
authors.initials: B
authors.lastname: Agca
authors.forename: Ulku A
authors.initials: UA
authors.lastname: Turkmen
authors.forename: Deniz
authors.initials: D
authors.lastname: Ozcan
authors.forename: Musa K
authors.initials: MK
authors.lastname: Sari
mesh_terms: D000818:Animals; D018988:Bacterial Translocation; D005260:Female; D007422:Intestines / Q000098:blood supply*; D007511:Ischemia / Q000209:etiology*; D010535:Laparoscopy / Q000009:adverse effects; D010538:Peritonitis; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011312:Pressure / Q000009:adverse effects*; D051381:Rats; D017208:Rats, Wistar