title: Improved solvent extraction procedure and high-performance liquid chromatography-evaporative light-scattering detector method for analysis of polar lipids from dairy materials.
abstract: A normal-phase high-performance liquid chromatography-evaporative light-scattering detector method employing dichloromethane, methanol, and acetic acid/triethylamine buffer as the mobile phase was developed for analysis of polar lipids (PLs). This method was applicable for analysis of PLs from both dairy materials and soy lecithin. All of the PLs of interest such as glycolipids, phospholipids, and sphingomyelin were well separated with a total run time of 22.5 min and without necessitating the removal of neutral lipids beforehand. Peak retention times were stable, and the method was reproducible. In this study, a modified method of using solvents for extraction of PLs from dairy matrices was also investigated. The modified method offered higher extraction efficiency, consumed less time, and in some cases saved solvent use.
authors.affiliation: Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Ghent University , Coupure links 653, 9000 Gent, Belgium. le.trungthien@ugent.be
authors.forename: Thien Trung
authors.initials: TT
authors.lastname: Le
authors.forename: Jelena
authors.initials: J
authors.lastname: Miocinovic
authors.forename: Tuyet Mai
authors.initials: TM
authors.lastname: Nguyen
authors.forename: Roeland
authors.initials: R
authors.lastname: Rombaut
authors.forename: John
authors.initials: J
authors.lastname: van Camp
authors.forename: Koen
authors.initials: K
authors.lastname: Dewettinck
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003611:Dairy Products / Q000032:analysis*; D020404:Glycerophospholipids / Q000032:analysis; D006017:Glycolipids / Q000032:analysis; D008027:Light; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012997:Solvents; D013109:Sphingomyelins / Q000032:analysis
chemical_list: D020404:Glycerophospholipids; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D012997:Solvents; D013109:Sphingomyelins