title: Aneuploidy drives genomic instability in yeast.
abstract: Aneuploidy decreases cellular fitness, yet it is also associated with cancer, a disease of enhanced proliferative capacity. To investigate one mechanism by which aneuploidy could contribute to tumorigenesis, we examined the effects of aneuploidy on genomic stability. We analyzed 13 budding yeast strains that carry extra copies of single chromosomes and found that all aneuploid strains exhibited one or more forms of genomic instability. Most strains displayed increased chromosome loss and mitotic recombination, as well as defective DNA damage repair. Aneuploid fission yeast strains also exhibited defects in mitotic recombination. Aneuploidy-induced genomic instability could facilitate the development of genetic alterations that drive malignant growth in cancer.
authors.affiliation: David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute (HHMI), Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Sheltzer
authors.forename: Heidi M
authors.initials: HM
authors.lastname: Blank
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Pfau
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Tange
authors.forename: Benson M
authors.initials: BM
authors.lastname: George
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Humpton
authors.forename: Ilana L
authors.initials: IL
authors.lastname: Brito
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hiraoka
authors.forename: Osami
authors.initials: O
authors.lastname: Niwa
authors.forename: Angelika
authors.initials: A
authors.lastname: Amon
mesh_terms: D000782:Aneuploidy; D020090:Chromosome Segregation; D015825:Chromosomes, Fungal / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D016681:Genome, Fungal; D042822:Genomic Instability; D016296:Mutagenesis; D009154:Mutation; D009369:Neoplasms / Q000235:genetics; D010641:Phenotype; D051721:Rad52 DNA Repair and Recombination Protein / Q000235:genetics; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D004271:DNA, Fungal; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins