title: Mediator complex proteins are required for diverse developmental processes.
abstract: The Mediator complex serves a crucial function in gene regulation, forming a link between gene-specific transcription factors and RNA polymerase II. Most protein-coding genes therefore require Mediator complex activity for transcriptional regulation. Given the essential functions performed by Mediator complex proteins in gene regulation, it is not surprising that mutations in Mediator complex genes disrupt animal and plant development. What is more intriguing is that the phenotypes of individual Mediator complex mutants are distinct from each other, demonstrating that certain developmental processes have a greater requirement for specific Mediator complex genes. Additionally, the range of developmental processes that are altered in Mediator complex mutants is broad, affecting a variety of cell types and physiological systems. Gene expression defects in Mediator complex mutants reveal distinct roles for individual Mediator proteins in transcriptional regulation, suggesting that the deletion of one Mediator complex protein does not interfere with transcription in general, but instead alters the expression of specific target genes. Mediator complex proteins may have diverse roles in different organisms as well, as mutants in the same Mediator gene in different species can display dissimilar phenotypes.
authors.affiliation: Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK. kathryn.hentges@manchester.ac.uk
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Hentges
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D056892:Mediator Complex / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D010641:Phenotype; D012319:RNA Polymerase II / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D056892:Mediator Complex; D014157:Transcription Factors; D012319:RNA Polymerase II