title: Determination of specific neuropeptides modulation time course in a rat model of osteoarthritis pain by liquid chromatography ion trap mass spectrometry.
abstract: Animal models are useful to evaluate pharmacological therapies to alleviate joint pain. The present study characterized central neuropeptides modulation in the monoiodoacetate (MIA) rat model. Animals receiving a single 3mg MIA injection were euthanized at 3, 7, 14, 21 and 28 days post injection. Spinal cords were analyzed by liquid chromatography ion trap mass spectrometry. Up-regulations of the calcitonin gene-related peptide and substance P were observed starting on days 7 and 28 respectively, whereas big dynorphin(₁₋₃₂) content decreased significantly on day 14 in comparison to control animals (P<0.05). Preclinical drug evaluations using this model should be conducted between 7 and 21 days post injection when the lesions resemble most to human osteoarthritis.
authors.affiliation: Département de Biomédecine Vétérinaire, Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Rue Sicotte, Saint-Hyacinthe, Québec J2S2M2, Canada.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Ferland
authors.forename: Floriane
authors.initials: F
authors.lastname: Pailleux
authors.forename: Pascal
authors.initials: P
authors.lastname: Vachon
authors.forename: Francis
authors.initials: F
authors.lastname: Beaudry
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D004195:Disease Models, Animal; D004399:Dynorphins / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D019807:Iodoacetic Acid / Q000494:pharmacology; D007719:Knee Joint / Q000378:metabolism / Q000473:pathology; D008297:Male; D009479:Neuropeptides / Q000032:analysis*; D010003:Osteoarthritis / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D010146:Pain / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry; D013373:Substance P / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D009479:Neuropeptides; D013373:Substance P; D004399:Dynorphins; C045485:dynorphin (1-32); D015740:Calcitonin Gene-Related Peptide; D019807:Iodoacetic Acid