title: Construction of Ipr1 expression vector and development of cloned embryos in vitro.
abstract: The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP-EGFP-Ipr1was constructed. Bovine fetal fibroblasts were transfected with pSP-EGFP-Ipr1. The green fluorescent protein (GFP)-expressing cells were selected and transferred into enucleated bovine oocytes. Then, the rates of oocyte cleavage and blastocyst formation of transgenic cells and non-transgenic cells were observed, respectively. The results showed that reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive. This study may provide cloned embryos for the production of anti-tuberculosis transgenic animals.
authors.affiliation: Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China. Zhy1956p@163.com
authors.forename: Yongli
authors.initials: Y
authors.lastname: Song
authors.forename: Xiaoning
authors.initials: X
authors.lastname: He
authors.forename: Song
authors.initials: S
authors.lastname: Hua
authors.forename: Jie
authors.initials: J
authors.lastname: Lan
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Liu
authors.forename: Pang
authors.initials: P
authors.lastname: Cheng
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.forename: Jixia
authors.initials: J
authors.lastname: Li
authors.forename: Xiaoying
authors.initials: X
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D019976:Cloning, Organism / Q000379:methods*; D005260:Female; D005347:Fibroblasts; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007621:Karyotyping; D008810:Mice, Inbred C57BL; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000502:physiology; D016133:Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection
chemical_list: C465035:Ifi75 protein, mouse; D015534:Trans-Activators; D049452:Green Fluorescent Proteins