title: [Construction and analysis of one kind of chitosan-coated BMP-2 nanoparticles as genetic carrier].
abstract: OBJECTIVE
  To construct an excellent kind of chitosan-coated BMP-2 nanoparticles as genetic carrier.
  
  
  METHODS
  Chitosan-coated BMP-2 nanoparticles as genetic carrier, including C-B50 group, C-B100 group and C-B200 group, were constructed through re-coacervation and gene recombination methods. This carrier was studied by using morphological observation, enveloping test, anti- DNase I digested test and MTT assay.
  
  
  RESULTS
  One kind of excellent chitosan-coated BMP-2 nanoparticles as genetic carrier was constructed. Particle diameter was about (90 +/- 20) nm. Enveloping ratio of C-B50 group, C-B100 group and C-B200 group was 76.31% + 1.58%, 84.49% + 1.81% and 81.69% + 1.77%, respectively. Genetic carrier could prevent objective gene from DNase I digestion. The growth inhibiting ratio to bone marrow stem cells was increased as the increase of genetic carrier dose and contact time (P < 0.05), but maximal dose of carrier had no obvious toxic effect on cells growth in vitro.
  
  
  CONCLUSIONS
  The constructed chitosan-coated BMP-2 nanoparticle material was a promising genetic carrier in bone tissue engineering.
authors.affiliation: Department of Prosthodontics, Hengha Stomatology Hospital, Hangzhou 310006, China.
authors.forename: Meng-Tao
authors.initials: MT
authors.lastname: Liu
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Zhang
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Zhai
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005822:Genetic Vectors; D006801:Humans; D053758:Nanoparticles; D023822:Tissue Engineering / Q000379:methods*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D020099:Coated Materials, Biocompatible; D048271:Chitosan