title: A rapid assay for miRNA maturation by using unmodified pre-miRNA.
abstract: Without labeling RNA: A new rapid assay for micro-RNA maturation was developed. The assay depends on the cleavage of unmodified pre-miRNAs and the subsequent amplification of the mature miRNA by rolling circle amplification (see accompanying scheme).
authors.affiliation: Institut für Chemie, Humboldt Universität zu Berlin, Brook-Taylor-Strasse 2, 12489 Berlin, Germany.
authors.forename: Saskia
authors.initials: S
authors.lastname: Neubacher
authors.forename: Claudine M
authors.initials: CM
authors.lastname: Dojahn
authors.forename: Christoph
authors.initials: C
authors.lastname: Arenz
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D057075:Enzyme Assays / Q000191:economics / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism; D013997:Time Factors
chemical_list: D035683:MicroRNAs; D011994:Recombinant Proteins; D043244:Ribonuclease III