title: [Development of a method for estimation of citalopram and desmethylcitalopram in nails and hair and its usefulness in forensic toxicology].
abstract: The report presents the possibility of using an alternative material of determining citalopram and its metabolite (desmethylcitalopram) in hair and nails. Citalopram (Cipramil, Citaratio, Citaxin, Oropram, Cital, Cilon, Aurex) is an antidepressant drug of the selective serotonin reuptake inhibitor (SSRI) class, employed in treatment of depression, prevention of depressive disorders recurrence and in some anxiety disorders. The investigations were performed using liquid chromatography coupled with electrospray-ionization mass spectrophotometry (LC-ESI-MS). In the course of the study, the authors developed a method for isolation and identification of Citaprolam and its metabolite (desmethylcitaprolam) from hair and nails. Determination were performed in hair and nail samples collected from individuals who had been administered citalopram in therapeutic doses at least for 12 months before sample collection. Hair and nail samples were obtained 4, 6, 9 and 15 months after discontinuing drug administration. The concentration of citalopram in nails was 0.40-10.49 ng/mg and the concentration of desmethylcitalopram was 0.32-3.70 ng/mg. In hair, citalopram concentration was 1.04-8.69 ng/mg and for desmethylcitalopram, the concentration range was 0.07-1.27 ng/mg.
authors.affiliation: Z Katedry i Zaktadu Medycyny Sadowej Uniwersytetu Mikołaja Kopernika w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pufal
authors.forename: Marzena
authors.initials: M
authors.lastname: Sykutera
authors.forename: Teresa
authors.initials: T
authors.lastname: Nowacka
authors.forename: Anna
authors.initials: A
authors.lastname: Stefanowicz
authors.forename: Karol
authors.initials: K
authors.lastname: Sliwka
mesh_terms: D014150:Antipsychotic Agents / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D015283:Citalopram / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D003866:Depressive Disorder / Q000188:drug therapy; D053593:Forensic Toxicology / Q000379:methods*; D006197:Hair / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009262:Nails / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D017367:Selective Serotonin Reuptake Inhibitors / Q000032:analysis
chemical_list: D014150:Antipsychotic Agents; D015283:Citalopram; D017367:Selective Serotonin Reuptake Inhibitors; C046304:desmethylcitalopram