title: RNA sequencing.
abstract: This chapter describes the RNA sequencing (RNA-Seq) protocol, whereby RNA from yeast cells is prepared for sequencing on an Illumina Genome Analyzer. The protocol can easily be altered to use RNA from a different organism. This chapter covers RNA extraction, cDNA synthesis, cDNA fragmentation, and Illumina cDNA library generation and contains some brief remarks on bioinformatic analysis.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.
authors.forename: Karl
authors.initials: K
authors.lastname: Waern
authors.forename: Ugrappa
authors.initials: U
authors.lastname: Nagalakshmi
authors.forename: Michael
authors.initials: M
authors.lastname: Snyder
mesh_terms: D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D015723:Gene Library; D023281:Genomics; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D018076:DNA, Complementary; D012331:RNA, Fungal