title: Application of dermal microdialysis for the determination of bioavailability of clobetasol propionate applied to the skin of human subjects.
abstract: Dermal microdialysis was used to assess the bioavailability of a topical corticosteroid, clobetasol propionate, following application onto the skin of human subjects. The penetration of clobetasol propionate from a 4% m/v ethanolic solution applied onto 4 sites on one forearm of healthy human volunteers was studied. A lipid emulsion, Intralipid®, was used as the perfusate and linear microdialysis probes with a 2-kDa cutoff were inserted intradermally at the designated sites. The results indicated that Intralipid could be used as a suitable perfusate for in vivo microdialysis of this lipophilic drug of interest. Furthermore, the study clearly demonstrated the application of dermal microdialysis as a valuable tool to assess the bioavailability/bioequivalence of clobetasol propionate penetration into the skin following topical application.
authors.affiliation: Rhodes University, Grahamstown, South Africa.
authors.forename: W L
authors.initials: WL
authors.lastname: Au
authors.forename: M F
authors.initials: MF
authors.lastname: Skinner
authors.forename: E
authors.initials: E
authors.lastname: Benfeldt
authors.forename: R K
authors.initials: RK
authors.lastname: Verbeeck
authors.forename: I
authors.initials: I
authors.lastname: Kanfer
mesh_terms: D000279:Administration, Cutaneous; D000293:Adolescent; D000327:Adsorption; D000328:Adult; D019540:Area Under Curve; D001682:Biological Availability; D002990:Clobetasol / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D005938:Glucocorticoids / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008055:Lipids; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D012867:Skin / Q000378:metabolism* / Q000648:ultrastructure; D012869:Skin Absorption; D012965:Sodium Chloride; D055815:Young Adult
chemical_list: D005938:Glucocorticoids; D008055:Lipids; D012965:Sodium Chloride; D002990:Clobetasol