title: Click maltose as an alternative to reverse phase material for desalting glycopeptides.
abstract: Desalting peptides before mass spectrometry analysis is important because salts lead to adduct formation, increased chemical noise and ion suppression effect. A high concentration of salt can clog nanoelectrospray ionization (ESI) emitters. The reverse phase C18 material is commonly used to desalt peptides because of its high binding capacity. However, peptides with high hydrophilicity, such as glycopeptides, are not retained well on this material, resulting in the loss of peptide information. To improve the efficiency of glycopeptide desalting, we introduced a hydrophilic interaction chromatography (HILIC)-based material named click maltose. Four glycoproteins, horseradish peroxidase (HRP), human serum immunoglobulin G (IgG), bovine ribonuclease B (RNase B), and α-1 acid glycoprotein (AGP) were chosen as models and their glycopeptides were desalted with click maltose, AQ C18, Empore C18 and ZipTip C18. Click maltose as a HILIC material exhibited better performance than the other three C18 materials for both number of targeted glycopeptides and their corresponding intensities. In addition, accurate glycopeptide profiling was achieved with click maltose desalting regardless of peptide lengths and glycan types.
authors.affiliation: The Second Affiliated Hospitals of Dalian Medical University, Dalian, 116044, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Xiuling
authors.initials: X
authors.lastname: Li
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Long
authors.initials: L
authors.lastname: Yu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zou
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D002417:Cattle; D057930:Click Chemistry; D006020:Glycopeptides / Q000032:analysis*; D006801:Humans; D008320:Maltose / Q000032:analysis*
chemical_list: D006020:Glycopeptides; D008320:Maltose