title: Studying permeability in a commonly used epithelial cell line: T84 intestinal epithelial cells.
abstract: The integrity, or barrier function, of the intestinal epithelium is of paramount importance in -maintaining good health. This is largely imparted by a single layer of epithelial cells linked by the transmembrane tight junction protein complex near their apical surface. Disruption of epithelial permeability via the tight junctions can contribute to disease progression. The cytokine IFNγ is involved in many inflammatory processes and has been shown to dramatically increase permeability via changes at the tight junction in experimental models. One of its key effectors is the transcription factor, -IRF-1. In our studies of the role of IRF-1 in barrier function using the human T84 intestinal epithelial cell monolayer model, we have found that induction of IRF-1 alone is insufficient to change permeability and that if IRF-1 is involved in mediating the permeability effects of IFNγ, then other factors must also be required.
authors.affiliation: Women's and Children's Health Research Institute, North Adelaide, SA, Australia.
authors.forename: Rino P
authors.initials: RP
authors.lastname: Donato
authors.forename: Adaweyah
authors.initials: A
authors.lastname: El-Merhibi
authors.forename: Batjargal
authors.initials: B
authors.lastname: Gundsambuu
authors.forename: Kai Yan
authors.initials: KY
authors.lastname: Mak
authors.forename: Emma R
authors.initials: ER
authors.lastname: Formosa
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Abbott
authors.forename: Barry C
authors.initials: BC
authors.lastname: Powell
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D003911:Dextrans / Q000032:analysis; D015194:Diffusion Chambers, Culture; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D050856:Interferon Regulatory Factor-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D062793:Occludin; D010539:Permeability; D010957:Plasmids; D019108:Tight Junctions / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
chemical_list: D003911:Dextrans; D050856:Interferon Regulatory Factor-1; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; C015219:fluorescein isothiocyanate dextran; D007371:Interferon-gamma; D016650:Fluorescein-5-isothiocyanate