title: regulation of the invasion and metastasis of human glioma cells by polypeptide N-acetylgalactosaminyltransferase 2.
abstract: Polypeptide, N-acetylgalactosaminyltransferase 2 (ppGalNAc-T2), is a member of the ppGalNAcT family which is the initial glycosyltransferase in O-glycan synthesis. Our previous studies have demonstrated that ppGalNAc-T2 plays a vital role in the process of tumor emergence and development. In this study, we aimed to determine whether ppGalNAc-T2 is correlated with the invasion and metastasis of human glioma cells. PpGalNAc-T2 sense vectors and interference vectors constructed in our laboratory were successfully transfected into SHG44 and U251 cells, which were observed through fluorescence microscopy, RT-PCR and western blot analysis. The metastasis of U251 cells was determined using a wound healing assay, and ppGalNAc-T2 was found to inhibit cell migration. By RT-PCR, glioma cell invasion assay showed that the expression of MMP-2 was increased in ppGalNAc-T2-knockdown cells, while the expression of MMP-9 and TGF-β1 was decreased in ppGalNAc-T2-overexpressing cells at the mRNA level. Meanwhile, the expression of TIMP-2 at the mRNA level was contrary to MMP-2. In summary, our findings indicate that ppGalNAc-T2 plays a poten-tial role in glioma cell migration and invasion, suggesting a new molecular therapeutic target for human malignant glioma treatment.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College, Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Lingyan
authors.initials: L
authors.lastname: Yang
authors.forename: Meifang
authors.initials: M
authors.lastname: Jin
authors.forename: Lan
authors.initials: L
authors.lastname: Xu
authors.forename: Shiliang
authors.initials: S
authors.lastname: Wu
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D000097763:Polypeptide N-acetylgalactosaminyltransferase
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D017350:N-Acetylgalactosaminyltransferases; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; D053773:Transforming Growth Factor beta1; D000097763:Polypeptide N-acetylgalactosaminyltransferase