title: [Primary study on origination of bone marrow abnormal clones in patients with myelodysplastic syndrome].
abstract: The study was aimed to investigate the origination of abnormal clones in hematopoietic cells of MDS patients. That is to say if there are abnormal clones in CD34(+)CD38(-) and CD34(+)CD38(+) cells and their proportions in MDS patients. Immuno-magnetic bead technique was used to sort CD34(+)CD38(-) and CD34(+)CD38(+) in bone marrow mononuclear cells of 9 MDS patients with chromosome abnormalities (four cases with trisomy 8, 1 case with trisomy 8 complex karyotype, 2 cases with 5q(-), 1 case with 5q(-)complex karyotype, 1 case with 5q(-) accompanying trisomy 8) and smears were made respectively. Then the percentage of abnormal clones in CD34(+)CD38(-) and CD34(+)CD38(+) cells were compared by using FISH. The results indicated that abnormal clones were involved in the two population cells in 9 patients. The percentage of abnormal clones in CD34(+)CD38(-) cells (41.8 ± 8.4%)was obviously lower than that in CD34(+)CD38(+) cells(72.4 ± 7.7%) (p < 0.001), and the percentage of abnormal clones in karyocytes was 70.8 ± 9.2%. It is concluded the abnormal clones of bone marrow hematopoietic cells may originate from stem cell stage in MDS patients with 5q(-) and +8, and the abnormal clones are predominant at stage of progenitors.
authors.affiliation: Suzhou University Medical College, Suzhou, Jiangsu Province, China.
authors.forename: Qing-Xia
authors.initials: QX
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: He
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Wu
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34; D001854:Bone Marrow Cells / Q000166:cytology*; D002869:Chromosome Aberrations; D002999:Clone Cells; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*
chemical_list: D018952:Antigens, CD34; D051997:ADP-ribosyl Cyclase 1