title: Neural cell adhesion molecule differentially interacts with isoforms of the fibroblast growth factor receptor.
abstract: The fibroblast growth factor receptor (FGFR) can be activated through direct interactions with various fibroblast growth factors or through a number of cell adhesion molecules, including the neural cell adhesion molecule (NCAM). We produced recombinant proteins comprising the ligand-binding immunoglobulin-like modules 2 and 3 of FGFR1b, FGFR1c, FGFR2b, FGFR2c, FGFR3b, FGFR3c, and FGFR4, and found that all FGFR isoforms, except for FGFR4, interacted with NCAM. The binding affinity of NCAM-FGFR interactions was considerably higher for splice variant 'b' than for splice variant 'c'. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling through FGFR.
authors.affiliation: Department of Neuroscience and Pharmacology, Protein Laboratory, University of Copenhagen, Copenhagen N, Denmark.
authors.forename: Claus
authors.initials: C
authors.lastname: Christensen
authors.forename: Vladimir
authors.initials: V
authors.lastname: Berezin
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Bock
mesh_terms: D000818:Animals; D008297:Male; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D020349:Surface Plasmon Resonance
chemical_list: D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms; D017468:Receptors, Fibroblast Growth Factor; D011994:Recombinant Proteins