title: Expression of liquoral neuroprotection markers in children with acute lymphoblastic leukemia.
abstract: Brain damage related to intrathecal methotrexate in children with acute lymphoblastic leukemia (ALL) is still unclear. Neuroinflammatory mechanisms and intracerebral production of specific biomarkers, play a key role in determining neuroprotective mechanisms after brain injury. To determine whether the CSF concentrations of neuron-specific enolase (NSE), neurotrophic factors and doublecortin (DCX) are influenced by repeated intrathecal methotrexate administrations, we prospectively collected CSF samples from 10 children with ALL and 10 controls. Our results showed an increased expression of the liquoral markers. This up-regulation could be interpreted as a neuroprotective response of the brain against the neuronal damages induced by MTX.
authors.affiliation: Department of Pediatric Neurosciences, Catholic University of Rome, Rome, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Chiaretti
authors.forename: Antonio
authors.initials: A
authors.lastname: Ruggiero
authors.forename: Paola
authors.initials: P
authors.lastname: Coccia
authors.forename: Alessia
authors.initials: A
authors.lastname: Antonelli
authors.forename: Filomena
authors.initials: F
authors.lastname: Pierri
authors.forename: Giovanni
authors.initials: G
authors.lastname: Barone
authors.forename: Giorgio
authors.initials: G
authors.lastname: Attinà
authors.forename: Laura
authors.initials: L
authors.lastname: Iuvone
authors.forename: Palma
authors.initials: P
authors.lastname: Maurizi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Riccardi
mesh_terms: D000293:Adolescent; D000964:Antimetabolites, Antineoplastic / Q000009:adverse effects; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid; D015153:Blotting, Western; D001930:Brain Injuries / Q000134:cerebrospinal fluid / Q000139:chemically induced / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008727:Methotrexate / Q000009:adverse effects*; D008869:Microtubule-Associated Proteins / Q000134:cerebrospinal fluid*; D009414:Nerve Growth Factors / Q000134:cerebrospinal fluid*; D009479:Neuropeptides / Q000134:cerebrospinal fluid*; D018696:Neuroprotective Agents / Q000134:cerebrospinal fluid*; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000473:pathology; D011379:Prognosis; D011446:Prospective Studies; D015854:Up-Regulation
chemical_list: D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; C000714328:DCX protein, human; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D009479:Neuropeptides; D018696:Neuroprotective Agents; D010751:Phosphopyruvate Hydratase; D008727:Methotrexate