title: [Anti-inflammatory and analgesic effects of saponins from D. zingiberensis C. H. Wright and diosgenin derivative on mice].
abstract: OBJECTIVE
  To determine the potential anti-inflammatory and analgesic activities of saponins from D. zingiberensis C. H. Wright and diosgenin derivative on xylene-induced acute ear edema in mice.
  
  
  METHODS
  3beta-2-acetoxy-benzoyl-diosgenin ester (ABDE) was synthesized by acylation of aspirin through two-step of reaction. The analgesic activities of ABDE and saponins were investigated through acetic acid-induced writhing response of the mice. The anti-inflammatory effects of saponins and ABDE were observed on the xylene-induced swelling ears of the mice.
  
  
  RESULTS
  The structure of ABDE was confirmed by 1H-NMR and ESI-MS. ABDE had significantly higher anti-inflammatory and analgesic activities than the comparison drug. The saponins demonstrated 7.92% and 14.51% anti-inflammatory activities at the dose of 126 mg/kg and 252 mg/kg, respectively, and 15.38% and 26.15% analgesic activities at the dose of 126 mg/kg and 252 mg/kg, respectively.
  
  
  CONCLUSIONS
  The saponins of D. zingiberensis C. H. Wright possess acute nonspecific anti-inflammatory and analgesic effects. The anti-inflammatory and analgesic effects of ABDE are equivalent to aspirin.
authors.affiliation: State Key Laboratory of Biotherapy, Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Mao-Hua
authors.initials: MH
authors.lastname: Ma
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D011897:Random Allocation; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D012503:Saponins; D004144:Diosgenin