title: Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry.
abstract: In this work, we developed a novel class-specific immunoaffinity column for the natural isoprenoid cytokinins (CTKs) by using trans-zeatin riboside as the hapten to generate a complete antigen. By combination with a mixed-mode solid phase extraction step for pre-cleanup and a high performance liquid chromatography-quadrupole-time of flight mass spectrometry for the quantification, an efficient analytical protocol was established which allowed simultaneous quantification of eight endogenous isoprenoid CTKs in Arabidopsis thaliana leaves with a wide linear range from 25 to 500pg/g fresh weight and a detection limit of 12.5pg/g fresh weight. The method will be very useful for comprehensive research on the networks of signaling interactions of the active phytohormones and their regulation of the plant functions.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Zhu
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D001667:Binding, Competitive; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D003583:Cytokinins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D007541:Isopentenyladenosine / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D010937:Plant Growth Regulators / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012680:Sensitivity and Specificity; D013729:Terpenes / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators; D013729:Terpenes; D007541:Isopentenyladenosine; C009699:zeatin riboside