title: Alterations in protein and gene expression within the barrel cortices of ZnT3 knockout mice: experience-independent and dependent changes.
abstract: Much evidence exists for the involvement of vesicular zinc in neurotransmission and cortical plasticity. Recent studies have reported that mice deficient in zinc transporter-3 protein (ZnT3) and thus, vesicular zinc, have significant behavioural and biochemical deficits. Here, we examined whether phenotypic differences existed in the barrel cortices of ZnT3 KO mice using functional proteomics and quantitative PCR. Additionally, by manipulating whisker input, we also investigated experience-dependent changes in protein and gene expression, thereby assaying how cortical plasticity is different in the absence of vesicular zinc. The GABA metabolizing protein ABAT was observed in lower abundances consistently in KO mice. Several presynaptic proteins were identified that were abundant in differing amounts between the WT and KO groups in an experience-dependent manner. At baseline, we observed a decrease in the relative expression of Dlg4, Grin2a, Mt3, and Ntrkb genes in KO mice. The reduced expression of Nrtkb persisted with whisker plucking. These data demonstrate that fundamental changes in the expression of proteins and genes important in neurotransmission occur in the absence of vesicular zinc. Furthermore, the complement of experience-dependent changes were different between WT and KO mice, indicating that the lack of vesicular zinc affects the process of cortical plasticity.
authors.affiliation: Department of Psychology, University of Calgary, Calgary, AB, Canada.
authors.forename: Amy S
authors.initials: AS
authors.lastname: Nakashima
authors.forename: R Hussain
authors.initials: RH
authors.lastname: Butt
authors.forename: Richard H
authors.initials: RH
authors.lastname: Dyck
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D027682:Cation Transport Proteins; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics*; D026901:Membrane Transport Proteins; D000090023:Metallothionein 3; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000235:genetics; D013003:Somatosensory Cortex / Q000254:growth & development* / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology; D015032:Zinc / Q000172:deficiency / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000090023:Metallothionein 3; C000715430:Mt3 protein, mouse; C491177:Slc30a3 protein, mouse; D015032:Zinc