title: Intersession repeatability of a novel nasal lavage technique.
abstract: This study describes a novel nasal lavage method using a pediatric tracheostomy tube and examines intersession repeatability for several clinically and technically relevant parameters. Fourteen healthy subjects were included in this study. Both nasal cavities were washed using a standard amount of saline solution (7 mL) via a pediatric tracheostomy tube, and the 2 samples were pooled for measurement of cytokine concentrations and cell count. Recovery volume was also recorded. For each subject, measurements were repeated on 5 consecutive days. Intersession repeatability of recovery volume, cell count, and cytokine concentrations interleukin (IL)-6 and IL-8 were expressed in terms of mean coefficient of variation, intraclass correlation coefficient, and interitem correlations. Intraclass correlation coefficients and interitem correlation coefficients indicated almost perfect agreement for cell count and IL-8 concentrations. Recovery volume and IL-6 concentrations were more variable. The mean coefficient of variation was low for cell count (2%), IL-8 concentration (3%), and recovery volume (3%), whereas the mean percentage recovery was high (87%). This newly developed nasal lavage technique is repeatable over successive sessions for cytokine concentrations and cell counts in nasal secretions of healthy subjects. This method might be valuable in the study of inflammatory conditions involving the upper respiratory tract.
authors.affiliation: Academic Unit of Respiratory Medicine, University College London Medical School, Royal Free Campus, London, UK. alahmari87@hotmail.com
authors.forename: Mohammed D
authors.initials: MD
authors.lastname: AlAhmari
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Sapsford
authors.forename: Jadwiga A
authors.initials: JA
authors.lastname: Wedzicha
authors.forename: John R
authors.initials: JR
authors.lastname: Hurst
mesh_terms: D000328:Adult; D002452:Cell Count; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D055556:Nasal Lavage / Q000295:instrumentation / Q000379:methods*; D017817:Nasal Lavage Fluid / Q000166:cytology / Q000276:immunology; D015203:Reproducibility of Results; D012140:Respiratory Tract Diseases / Q000175:diagnosis / Q000276:immunology; D014139:Tracheostomy / Q000295:instrumentation; D057170:Translational Research, Biomedical
chemical_list: C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8