title: Improved long-term protection against Mycobacterium tuberculosis Beijing/W in mice after intra-dermal inoculation of recombinant BCG expressing latency associated antigens.
abstract: Bacille Calmette-Guérin (BCG) is the vaccine against tuberculosis (TB), but has varied efficacy in different geographical locations. Recombinant strategies to genetically modify the organism to enhance the quality of the immune response have aimed at improving BCG efficacy. Here we describe such a strategy using rBCGΔureC∷hly expressing defined latency-associated antigens and test this construct for long-term protection against an isolate of the Mycobacterium tuberculosis (Mtb) Beijing/W lineage. Expression of the antigens Rv2659c, Rv3407 and Rv1733c by rBCGΔureC∷hly improved long-term efficacy in both lung and spleen at day 200 post-infection after intradermal vaccination of mice. Our data support expression of Mtb latency associated antigens by rBCG to improve protection against Mtb.
authors.affiliation: Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany. reece@mpiib-berlin.mpg.de
authors.forename: Stephen T
authors.initials: ST
authors.lastname: Reece
authors.forename: Ali
authors.initials: A
authors.lastname: Nasser-Eddine
authors.forename: Jes
authors.initials: J
authors.lastname: Dietrich
authors.forename: Maik
authors.initials: M
authors.lastname: Stein
authors.forename: Ulrike
authors.initials: U
authors.lastname: Zedler
authors.forename: Sandra
authors.initials: S
authors.lastname: Schommer-Leitner
authors.forename: Tom H M
authors.initials: TH
authors.lastname: Ottenhoff
authors.forename: Peter
authors.initials: P
authors.lastname: Andersen
authors.forename: Stefan H E
authors.initials: SH
authors.lastname: Kaufmann
mesh_terms: D000818:Animals; D000941:Antigens; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001500:BCG Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D027682:Cation Transport Proteins; D015169:Colony Count, Microbial; D005260:Female; D007271:Injections, Intradermal; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000382:microbiology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D000941:Antigens; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D027682:Cation Transport Proteins; D014614:Vaccines, Synthetic; D000099173:Natural Resistance-associated Macrophage Protein 1