title: Expressed sequence tags from heat-shocked seagrass Zostera noltii (Hornemann) from its southern distribution range.
abstract: Predicted global climate change threatens the distributional ranges of species worldwide. We identified genes expressed in the intertidal seagrass Zostera noltii during recovery from a simulated low tide heat-shock exposure. Five Expressed Sequence Tag (EST) libraries were compared, corresponding to four recovery times following sub-lethal temperature stress, and a non-stressed control. We sequenced and analyzed 7009 sequence reads from 30min, 2h, 4h and 24h after the beginning of the heat-shock (AHS), and 1585 from the control library, for a total of 8594 sequence reads. Among 51 Tentative UniGenes (TUGs) exhibiting significantly different expression between libraries, 19 (37.3%) were identified as 'molecular chaperones' and were over-expressed following heat-shock, while 12 (23.5%) were 'photosynthesis TUGs' generally under-expressed in heat-shocked plants. A time course analysis of expression showed a rapid increase in expression of the molecular chaperone class, most of which were heat-shock proteins; which increased from 2 sequence reads in the control library to almost 230 in the 30min AHS library, followed by a slow decrease during further recovery. In contrast, 'photosynthesis TUGs' were under-expressed 30min AHS compared with the control library, and declined progressively with recovery time in the stress libraries, with a total of 29 sequence reads 24h AHS, compared with 125 in the control. A total of 4734 TUGs were screened for EST-Single Sequence Repeats (EST-SSRs) and 86 microsatellites were identified.
authors.affiliation: CCMAR-CIMAR, Universidade do Algarve, Gambelas; 8005-139 Faro; Portugal.
authors.forename: Sónia I
authors.initials: SI
authors.lastname: Massa
authors.forename: Gareth A
authors.initials: GA
authors.lastname: Pearson
authors.forename: Tânia
authors.initials: T
authors.lastname: Aires
authors.forename: Michael
authors.initials: M
authors.lastname: Kube
authors.forename: Jeanine L
authors.initials: JL
authors.lastname: Olsen
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Ester A
authors.initials: EA
authors.lastname: Serrão
authors.forename: Sophie
authors.initials: S
authors.lastname: Arnaud-Haond
mesh_terms: D005075:Biological Evolution; D057231:Climate Change; D003710:Demography; D020224:Expressed Sequence Tags; D005420:Flavoproteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D018895:Microsatellite Repeats; D018384:Oxidative Stress; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D035962:Zosteraceae / Q000235:genetics / Q000378:metabolism*
chemical_list: D005420:Flavoproteins; D010940:Plant Proteins; C071072:vestitone reductase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D019696:Peptidylprolyl Isomerase; D005974:Glutamate-Ammonia Ligase