title: Bovine fetal DNA in the maternal circulation: Applications and implications.
abstract: OBJECTIVE
  The main aim of the present study was to detect bovine fetal DNA in the maternal circulation, a relatively unexplored subject in the literature.
  
  
  METHODS
  DNA was extracted from blood of 84 primipara cows (Bos indicus) at different gestational ages (30-270 days) and from 100 adult animals (50 males and 50 non-pregnant cows). The samples were analyzed using PCR with primers for TSPY gene.
  
  
  RESULTS
  Molecular results matched the fetal phenotypic gender in all 47 male and 37 female fetuses, including early pregnancy, and in control animals.
  
  
  CONCLUSIONS
  These results evidence a bovine transplacental fetal DNA passage.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. lemosdc@gmail.com
authors.forename: D C
authors.initials: DC
authors.lastname: Lemos
authors.forename: P L
authors.initials: PL
authors.lastname: Takeuchi
authors.forename: A F L
authors.initials: AF
authors.lastname: Rios
authors.forename: A
authors.initials: A
authors.lastname: Araújo
authors.forename: H C
authors.initials: HC
authors.lastname: Lemos
authors.forename: E S
authors.initials: ES
authors.lastname: Ramos
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000097:blood*; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D008297:Male; D008431:Maternal-Fetal Exchange / Q000235:genetics*; D009035:Mothers; D011247:Pregnancy; D011270:Pregnancy, Animal; D011296:Prenatal Diagnosis / Q000379:methods / Q000662:veterinary; D012737:Sex Factors
chemical_list: D004247:DNA