title: Using the meniscus in a capillary for small volume contact angle measurement in biochemical applications.
abstract: The contact angle is a sensitive parameter often used to define wettability. With the increasing movement toward smaller liquid volumes in many biochemical applications, a key challenge lies in how to perform measurements in the retainer holding the reagent for rapid evaluation and limited material loss. Here, we report a simple and robust method to determine the contact angle of small volumes using the microscopic imaging of a capillary meniscus that requires only the radius and meniscus height information. An error analysis of the measurement finds the method to be highly accurate. We also uncovered that illumination delivered from the liquid end of the capillary lights up the interface, thereby facilitating the measurement.
authors.affiliation: Laboratory for Optics, Acoustics & Mechanics, Monash University, Clayton VIC3800 Australia.
authors.forename: Brandon Huey-Ping
authors.initials: BH
authors.lastname: Cheong
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
mesh_terms: D008592:Menisci, Tibial / Q000737:chemistry*; D008853:Microscopy; D017830:Octoxynol / Q000737:chemistry; D014867:Water / Q000737:chemistry; D017155:Wettability
chemical_list: D014867:Water; D017830:Octoxynol