title: Down-regulation of SymRK correlates with a deficiency in vascular bundle development in Phaseolus vulgaris nodules.
abstract: The symbiotic interaction of legumes and rhizobia results in the formation of nitrogen-fixing nodules. Nodulation depends on the finely coordinated expression of a battery of genes involved in the infection and the organogenesis processes. After Nod factor perception, symbiosis receptor kinase (SymRK) receptor triggers a signal transduction cascade essential for nodulation leading to cortical cell divisions, infection thread (IT) formation and final release of rhizobia to the intracellular space, forming the symbiosome. Herein, the participation of SymRK receptor during the nodule organogenesis in Phaseolus vulgaris is addressed. Our findings indicate that besides its expression in the nodule epidermis, in IT, and in uninfected cells of the infection zone, PvSymRK immunolocalizes in the root and nodule vascular system. On the other hand, knockdown expression of PvSymRK led to the formation of scarce and defective nodules, which presented alterations in both IT/symbiosome formation and vascular system.
authors.affiliation: Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
authors.forename: Rosana
authors.initials: R
authors.lastname: Sánchez-López
authors.forename: David
authors.initials: D
authors.lastname: Jáuregui
authors.forename: Noreide
authors.initials: N
authors.lastname: Nava
authors.forename: Xóchitl
authors.initials: X
authors.lastname: Alvarado-Affantranger
authors.forename: Jesús
authors.initials: J
authors.lastname: Montiel
authors.forename: Olivia
authors.initials: O
authors.lastname: Santana
authors.forename: Federico
authors.initials: F
authors.lastname: Sanchez
authors.forename: Carmen
authors.initials: C
authors.lastname: Quinto
mesh_terms: D015536:Down-Regulation; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D027805:Phaseolus / Q000201:enzymology / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055170:Plant Root Nodulation; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D053204:Root Nodules, Plant / Q000201:enzymology / Q000254:growth & development*; D015398:Signal Transduction; D013559:Symbiosis
chemical_list: D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases