title: Edelfosine disturbs the sphingomyelin-cholesterol model membrane system in a cholesterol-dependent way - the Langmuir monolayer study.
abstract: Synthetic alkyl-lysophospholipids, represented by edelfosine (ED), reveal strong anticancer activity and therefore are promising drugs used in anticancer therapy. Primary target for edelfosine is cellular membrane, which is in contrast to traditional cytostatics affecting DNA. The mechanism of antitumor activity of edelfosine was hypothesized to be related to its accumulation in membrane rafts. Inspired by these findings, we have performed the Langmuir monolayer studies on the influence of edelfosine on systems composed of sphingomyelin (SM) and cholesterol (Chol), being the principal components of membrane rafts. Sphingomyelin-cholesterol proportion in monolayers was varied to reflect the composition of solely membrane rafts (SM/Chol=2:1) and contain excess of cholesterol (SM/Chol=1:1 and 1:2). Into these systems, edelfosine was added in various concentrations. The analysis of surface pressure-area isotherms, complemented with films visualization with Brewster angle microscopy (BAM) allowed us to compare the effect of edelfosine on condensation and ordering of SM/Chol monolayers. The results evidenced that the influence of ED on the interactions in model membranes and its fluidizing effect is highly cholesterol-dependent. The strongest decrease of monolayer ordering was observed for model raft system, while the excess of cholesterol present in the remaining mixtures was found to weaken the fluidizing effect of the drug.
authors.affiliation: Jagiellonian University, Faculty of Chemistry, Ingardena 3, 30-060 Kraków, Poland. hac@chemia.uj.edu.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hąc-Wydro
authors.forename: Patrycja
authors.initials: P
authors.lastname: Dynarowicz-Łątka
authors.forename: Paweł
authors.initials: P
authors.lastname: Wydro
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bąk
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D008567:Membranes, Artificial; D010742:Phospholipid Ethers / Q000737:chemistry*; D013109:Sphingomyelins / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D010742:Phospholipid Ethers; D013109:Sphingomyelins; C026659:edelfosine; D002784:Cholesterol