title: Influence of chirality of V(V) Schiff base complexes on DNA, BSA binding and cleavage activity.
abstract: New chiral V(V) Schiff base complexes (S)-[VO(OMe)L] and (R)-[VO(OMe)L] were synthesized and characterized by microanalysis, infrared (IR), UV-Visible, Circular dichroism (CD) spectroscopy and single crystal X-ray studies. The interaction of these complexes with calf thymus (CT) DNA and bovine serum albumin (BSA) protein showed chiral expression DNA/protein binding strength. The influence of chirality was also observed in cytotoxicity assay of Hep 2 cells. (R)-[VO(OMe)L] enantiomer exhibited higher binding constant (5 ± 1 × 10(5) M(-1)) as compared to (S)-[VO(OMe)L] (8 ± 1 × 10(4) M(-1)). The fluorescence quenching, thermal melting and viscosity data suggest DNA surface and/or groove binding nature of the complexes and electrophoresis studies also showed greater activity for (R)-[VO(OMe)L] in cleaving DNA and protein as against (S)-[VO(OMe)L].
authors.affiliation: Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute (CSMCRI), Council of Scientific & Industrial Research (CSIR), G. B. Marg, Bhavnagar 364 021, Gujarat, India. khan251293@yahoo.in
authors.forename: Noor-ul H
authors.initials: NU
authors.lastname: Khan
authors.forename: Nirali
authors.initials: N
authors.lastname: Pandya
authors.forename: Nabin Ch
authors.initials: NCh
authors.lastname: Maity
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Rajesh M
authors.initials: RM
authors.lastname: Patel
authors.forename: Rukhsana I
authors.initials: RI
authors.lastname: Kureshy
authors.forename: Sayed H R
authors.initials: SH
authors.lastname: Abdi
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
authors.forename: Satyabrata
authors.initials: S
authors.lastname: Das
authors.forename: Hari C
authors.initials: HC
authors.lastname: Bajaj
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008958:Models, Molecular; D009369:Neoplasms / Q000188:drug therapy; D011485:Protein Binding / Q000187:drug effects; D012545:Schiff Bases / Q000737:chemistry / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013237:Stereoisomerism; D014639:Vanadium / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D012545:Schiff Bases; D014639:Vanadium; D012710:Serum Albumin, Bovine; D004247:DNA; C427203:calf thymus DNA