title: [Role of interleukin-17 and Th17 cells in acute renal allograft rejection in mice].
abstract: OBJECTIVE
  To investigate the role of Thl7 cells and the cytokine interleukin-17 (IL-17) in acute allograft rejection in mice.
  
  
  METHODS
  Mouse models of kidney transplantation were randomly divided into rejection group and isograft group. On the post-operative day (POD) 3 and 7, we tested the serum IL-17 level using enzyme-linked immunosorbent assay and measured the number of Th17 cells in the renal grafts by flow cytometry. The grafts were harvested and fixed in 10% formalin to prepare paraffin sections for routine pathological inspection.
  
  
  RESULTS
  Compared to isograft group, the allograft group showed a significantly higher level of serum IL-17 on POD3 and POD7 (P<0.05), and the level of IL-17 is significantly higher on POD7 than on POD3 (P<0.05). The allograft group showed more infiltrating Th17 cells in the grafts on POD3 and POD7 (P<0.05), and the cell number was significantly greater on POD7 (P<0.05). Pathological examination also showed an increased severity of graft rejection with the post-transplantation time.
  
  
  CONCLUSIONS
  Thl7 cells may play an important role in the development of renal graft rejection. IL-17 may serve as a potential specific indicator for predicting allograft rejection.
authors.affiliation: Department of General Surgery Organ Transplantation, Second Xiangya Hospital, Central South University, Changsha, China. szz5858@medmail.com.cn
authors.forename: Zhong-zhou
authors.initials: ZZ
authors.lastname: Si
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Jie-qun
authors.initials: JQ
authors.lastname: Li
authors.forename: Hai-zhi
authors.initials: HZ
authors.lastname: Qi
authors.forename: Xu-biao
authors.initials: XB
authors.lastname: Xie
mesh_terms: D000818:Animals; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology*; D020381:Interleukin-17 / Q000097:blood*; D016030:Kidney Transplantation / Q000009:adverse effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D058504:Th17 Cells / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D020381:Interleukin-17