title: Accumulation of silver nanoparticles by cultured primary brain astrocytes.
abstract: Silver nanoparticles (AgNP) are components of various food industry products and are frequently used for medical equipment and materials. Although such particles enter the vertebrate brain, little is known on their biocompatibility for brain cells. To study the consequences of an AgNP exposure of brain cells we have treated astrocyte-rich primary cultures with polyvinylpyrrolidone (PVP)-coated AgNP. The incubation of cultured astrocytes with micromolar concentrations of AgNP for up to 24 h resulted in a time- and concentration-dependent accumulation of silver, but did not compromise the cell viability nor lower the cellular glutathione content. In contrast, the incubation of astrocytes for 4 h with identical amounts of silver as AgNO(3) already severely compromised the cell viability and completely deprived the cells of glutathione. The accumulation of AgNP by astrocytes was proportional to the concentration of AgNP applied and significantly lowered by about 30% in the presence of the endocytosis inhibitors chloroquine or amiloride. Incubation at 4 °C reduced the accumulation of AgNP by 80% compared to the values obtained for cells that had been exposed to AgNP at 37 °C. These data demonstrate that viable cultured brain astrocytes efficiently accumulate PVP-coated AgNP in a temperature-dependent process that most likely involves endocytotic pathways.
authors.affiliation: Center for Biomolecular Interactions Bremen, University of Bremen, PO Box 330440, D-28334 Bremen, Germany.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Luther
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Koehler
authors.forename: Joerg
authors.initials: J
authors.lastname: Diendorf
authors.forename: Matthias
authors.initials: M
authors.lastname: Epple
authors.forename: Ralf
authors.initials: R
authors.lastname: Dringen
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000166:cytology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011205:Povidone / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000378:metabolism*; D012835:Silver Nitrate / Q000494:pharmacology; D013054:Spectrophotometry, Atomic; D013696:Temperature; D013997:Time Factors
chemical_list: D012834:Silver; D012835:Silver Nitrate; D011205:Povidone; D005978:Glutathione