title: Transcriptomic analysis brings new insight into the biological role of the prion protein during mouse embryogenesis.
abstract: The biological function of the Prion protein remains largely unknown but recent data revealed its implication in early zebrafish and mammalian embryogenesis. To gain further insight into its biological function, comparative transcriptomic analysis between FVB/N and FVB/N Prnp knockout mice was performed at early embryonic stages. RNAseq analysis revealed the differential expression of 73 and 263 genes at E6.5 and E7.5, respectively. The related metabolic pathways identified in this analysis partially overlap with those described in PrP1 and PrP2 knockdown zebrafish embryos and prion-infected mammalian brains and emphasize a potentially important role for the PrP family genes in early developmental processes.
authors.affiliation: INRA, UMR1313, Génétique Animale et Biologie Intégrative, INRA, Jouy-en-Josas, France.
authors.forename: Manal
authors.initials: M
authors.lastname: Khalifé
authors.forename: Rachel
authors.initials: R
authors.lastname: Young
authors.forename: Bruno
authors.initials: B
authors.lastname: Passet
authors.forename: Sophie
authors.initials: S
authors.lastname: Halliez
authors.forename: Marthe
authors.initials: M
authors.lastname: Vilotte
authors.forename: Florence
authors.initials: F
authors.lastname: Jaffrezic
authors.forename: Sylvain
authors.initials: S
authors.lastname: Marthey
authors.forename: Vincent
authors.initials: V
authors.lastname: Béringue
authors.forename: Daniel
authors.initials: D
authors.lastname: Vaiman
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Provost
authors.forename: Hubert
authors.initials: H
authors.lastname: Laude
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vilotte
mesh_terms: D000818:Animals; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D047108:Embryonic Development / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D053263:Gene Regulatory Networks; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D011328:Prions / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D011328:Prions; D020033:Protein Isoforms; D029961:Zebrafish Proteins