title: [Definition and function identification of nucleus export signal of BRD7].
abstract: OBJECTIVE
  To localize and define the region of nucleus export signal (NES) on BRD7, and determine the role of this region in nucleus export of the external protein.
  
  
  METHODS
  Based on an in vitro expressed model of green fluorescence protein (GFP), we performed DNA walking analysis to set BRD7 into several sections according to the structural characteristics of BRD7, investigated the effect of different sections of BRD7 on nucleus export of GFP, defined the region of nucleus export signal sequence of BRD7, and further ascertained the content of amino acids in BRD7 and potential localization of BRD7 NES by bioinformatics.
  
  
  RESULTS
  B7C1 fragments ranged from aa219 to aa450 in BRD7 were found to target the external protein GFP into the cytoplasm detected by GFP direct fluorescence, which could be inhibited by NES inhibitor Leptomycin B (LMB). This region was rich in hydrophobic amino acid residues but no typical NES with characteristics of leucine-rich sequence by bioinformatics.
  
  
  CONCLUSIONS
  The region from aa219 to aa450 is primarily defined as an atypical NES in BRD7.
authors.affiliation: Cancer Research Institute, Central South University, Changsha 410078, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Chi
authors.initials: C
authors.lastname: Guo
authors.forename: Xiayu
authors.initials: X
authors.lastname: Li
authors.forename: Jiajin
authors.initials: J
authors.lastname: He
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xu
authors.forename: Heran
authors.initials: H
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Cao
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Li
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D049790:Nuclear Export Signals; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000092468:Bromodomain Containing Proteins
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D049452:Green Fluorescent Proteins; D049790:Nuclear Export Signals; D011994:Recombinant Proteins; C485694:BRD7 protein, human; D000092468:Bromodomain Containing Proteins