title: [Interaction of lactoferrin and its peptides with DPPC and DPPG liposomes studied by Raman spectroscopy].
abstract: Interaction of lactoferrin and its peptides LfcinB4-14 and LfampinB with dipalmitoylglycero-phosphocholine (DPPC) and dipalmitoylglycero-phosphoglycerol (DPPG) liposomes were studied by means of Raman spectroscopy. In our study, conformational changes in the phospholipid molecules were investigated by measuring the intensities of 2 847 and 2 882 cm(-1) Raman bands which are assigned to acyl chains' symmetric and asymmetric C-H stretching vibrations. The addition of lactoferrin and its peptides LfcinB4-14 and LfampinB caused a decrease in the 2 882 cm(-1) intensity of DPPG liposomes, thus the order parameter for the lateral interactions between chains S(lat) decreased from 0.19 to 0.17, 0.14 and 0.12 respectively. On the contrary, the intensities at 2 847 and 2 882 cm(-1) of DPPC liposomes were poorly affected by lactoferrin and its peptides. The results show that lactoferrin and its peptides present a stronger effect on the molecular structure and order degree of anionic lipid DPPG than that of zwitterionic lipid DPPC. This suggests that lactoferrin, LfcinB4-14 and LfampinB can interact and combine with the negatively charged DPPG liposomes by electrostatic interaction and perform its antibacterial activity. Besides, LfcinB4-14 and LfampinB can affect the lipid more strongly than lactoferrin.
authors.affiliation: College of Food Science & Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China. zw_7431@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Fa-Zheng
authors.initials: FZ
authors.lastname: Ren
authors.forename: Shao-Yang
authors.initials: SY
authors.lastname: Ge
authors.forename: Lu-Da
authors.initials: LD
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Jiang
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Mao
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Guo
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D007781:Lactoferrin / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D008081:Liposomes; D010455:Peptides; D010715:Phosphatidylglycerols; D015060:1,2-Dipalmitoylphosphatidylcholine; D007781:Lactoferrin; C030345:1,2-dipalmitoylphosphatidylglycerol