title: Streptomyces ghanaensis pleiotropic regulatory gene wblA(gh) influences morphogenesis and moenomycin production.
abstract: The wblA(gh) gene, encoding a homologue of the WhiB-family of proteins, was identified in the sequenced genome of moenomycin producer Streptomyces ghanaensis. Deletion of the gene blocked aerial mycelium sporulation and caused a 230% increase in moenomycins production. S. ghanaensis overexpressing SSFG-01620: a homologue of extracellular protease inhibitor SCO0762, whose expression in Streptomyces coelicolor is down-regulated by wblA: showed deficiencies in sporulation similar to that of wblA(gh) knockout strain. The wblA(gh) gene of S. ghanaensis appears to play a negative role in the control of moenomycin biosynthesis and is essential for sporulation.
authors.affiliation: Department of Genetic and Biotechnology, Ivan Franko National University of Lviv, Grushevskogo St. 4, Lviv 79005, Ukraine. Availl@i.ua
authors.forename: Mariia
authors.initials: M
authors.lastname: Rabyk
authors.forename: Bohdan
authors.initials: B
authors.lastname: Ostash
authors.forename: Yurij
authors.initials: Y
authors.lastname: Rebets
authors.forename: Suzanne
authors.initials: S
authors.lastname: Walker
authors.forename: Victor
authors.initials: V
authors.lastname: Fedorenko
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics*; D058685:Genetic Pleiotropy / Q000235:genetics*; D009024:Morphogenesis / Q000502:physiology*; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D012097:Repressor Proteins; C419802:moenomycin