title: Metallization and investigation of electrical properties of in vitro recrystallized mSbsC-eGFP assemblies.
abstract: Surface layer (SL) proteins are self-assembling nanosized arrays which can be recrystallized in solution or on surfaces. In this paper, we investigate the metallization, contact potential difference and conductivity of in vitro recrystallized mSbsC-eGFP tube-like assemblies for possible applications in nanobiotechnology. Treatment of mSbsC-eGFP tube-like structures with 150 mM Pt salt solution resulted in the formation of metallized SL assemblies decorated with Pt nanoparticles (∅ > 3 nm) which were closely packed and aggregated into metal clusters. Kelvin probe force microscopy (KPFM) measurements revealed that metallized and unmetallized SL templates showed different surface potential behaviours, demonstrating that the metal coating changes the electrostatic surface characteristics of SL assemblies. In situ conductivity measurements showed that unmetallized SL assemblies were not conductive. Metallized samples showed linear I-V dependence between - 1 and + 1 V with a conductivity of ∼ 10(3) S m( - 1).
authors.affiliation: Institut für Genetik, Technische Universität Dresden, 01217 Dresden, Germany. nuriye korkmaz@yahoo.com
authors.forename: Nuriye
authors.initials: N
authors.lastname: Korkmaz
authors.forename: Felix
authors.initials: F
authors.lastname: Börrnert
authors.forename: Denny
authors.initials: D
authors.lastname: Köhler
authors.forename: Rafael G
authors.initials: RG
authors.lastname: Mendes
authors.forename: Alicja
authors.initials: A
authors.lastname: Bachmatiuk
authors.forename: Mark H
authors.initials: MH
authors.lastname: Rümmeli
authors.forename: Bernd
authors.initials: B
authors.lastname: Büchner
authors.forename: Lukas M
authors.initials: LM
authors.lastname: Eng
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rödel
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D004560:Electricity; D056506:Geobacillus / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D008670:Metals; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins