title: Overexpression of human papillomavirus (HPV) type 16 oncoproteins promotes angiogenesis via enhancing HIF-1α and VEGF expression in non-small cell lung cancer cells.
abstract: HPV-16 infection may play an important role in the development of non-small cell lung cancer (NSCLC) among never-smokers. Due to the critical role of angiogenesis in NSCLC development, we describe here the effect of HPV-16 oncoproteins on angiogenesis in NSCLC and the underlying mechanisms. We found that overexpression of HPV-16 E6 and E7 oncoproteins in NSCLC cells significantly promoted angiogenesis both in vitro and in vivo, and correspondingly, an enhanced expression of HIF-1α and VEGF, important pro-angiogenic factors in tumor angiogenesis. Meanwhile, overexpression of HPV-16 oncoproteins also led to HIF-1α-dependent increases in the secretion of several other pro-angiogenic factors, including IL-8. Our findings suggest that HPV-16 oncoproteins contribute to the development of NSCLC possibly by promoting HIF-1α/VEGF-mediated tumor angiogenesis.
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Erying
authors.initials: E
authors.lastname: Zhang
authors.forename: Jingli
authors.initials: J
authors.lastname: Shi
authors.forename: Qunzhou
authors.initials: Q
authors.lastname: Zhang
authors.forename: Anh D
authors.initials: AD
authors.lastname: Le
authors.forename: Keyuan
authors.initials: K
authors.lastname: Zhou
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D002472:Cell Transformation, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D052162:Human papillomavirus 16 / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000821:virology*; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis*; D050725:Papillomavirus E7 Proteins / Q000096:biosynthesis*; D030361:Papillomavirus Infections / Q000378:metabolism*; D040081:Protein Array Analysis; D012097:Repressor Proteins / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; D042461:Vascular Endothelial Growth Factor A; C059731:oncogene protein E7, Human papillomavirus type 16