title: [Effect of seizure activity on subunit composition of Ca2+/calmodulin-dependent protein kinase II in hippocampus of Krushinskii-Molodkina rats].
abstract: Using a Western blot method it was been shown that a aontent of beta-CaMKII subunit was decreased on 40 % in hippocampus of Krushinskii-Molodkina rats (rats, genetically prone to audiogenic seizures) in comparison with normal Wistar rats. Additionally, we have investigate the temporal modifications of alpha/beta-CaMKII subunits in dependent from number audiogenic fits (n = 1 or n = 5). The level of beta-CaMKII in hippocampus of naive KM rats and KM rats in 24 h after single audiogenic seizure was not different, but it was increased on 56 % through 72 h after convulsion fit. In contrast, both 5-fold seizures and 20-fold seizures (audiogenic kindling) reduced the beta-CaMKII levels in comparison with naive group. The levels of alpha-subunit protein kinase in hippocampus these experimental groups of animals were not different. We suggest a hypothesis, that modifications alpha/beta ratio can be viewed as specific "homeostatic mechanism", which provide for neuronal function in hippocampus, thereby changing the rules governing synaptic plasticity in dependence from preliminary neuronal activity.
authors.forename: T A
authors.initials: TA
authors.lastname: Savina
authors.forename: T G
authors.initials: TG
authors.lastname: Shchipakina
authors.forename: O V
authors.initials: OV
authors.lastname: Godukhin
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000737:chemistry / Q000378:metabolism*; D006624:Hippocampus / Q000201:enzymology*; D008297:Male; D009474:Neurons / Q000201:enzymology; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000201:enzymology* / Q000503:physiopathology*; D013016:Sound / Q000009:adverse effects*
chemical_list: D021122:Protein Subunits; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2