title: Immunohistochemical analysis of bone resorption regulators (RANKL and OPG), angiogenic index, and myofibroblasts in syndrome and non-syndrome odontogenic keratocysts.
abstract: OBJECTIVE
  The aim of this study was to immunohistochemically analyse bone resorption regulators (receptor activator of nuclear factor kappa B ligand [RANKL] and osteoprotegerin [OPG]), angiogenic index, and myofibroblasts in Gorlin syndrome-related odontogenic keratocysts (SOKCs) and non-syndrome odontogenic keratocysts (NSOKCs).
  
  
  METHODS
  Twenty-two SOKCs, 22 primary NSOKCs, and eight recurrent NSOKCs were evaluated by immunohistochemistry using anti-RANKL and anti-OPG antibodies. The angiogenic index was determined by microvessel count (MVC) using anti-CD34 antibody. Anti-α-smooth muscle actin (α-SMA) antibody was used for the identification of myofibroblasts.
  
  
  RESULTS
  Analysis of the expression of RANKL and OPG in the epithelial lining and fibrous capsule did not reveal significant differences between groups (P>0.05). In the epithelial lining, the RANKL/OPG ratio was RANKL<OPG and RANKL=OPG in most primary NSOCKs (54.5%) and SOKCs (59.1%), respectively (P>0.05). In the fibrous capsule, the ratio was RANKL=OPG in most primary (81.8%) and recurrent NSOKCs (75.0%) and in most SOKCs (45.5%) (P>0.05). No significant differences in the angiogenic index or number of myofibroblasts were observed between primary NSOKCs, recurrent NSOKCs, and SOKCs (P>0.05).
  
  
  CONCLUSIONS
  The present results suggest that differences in the biological behaviour of SOKCs and NSOKCs may not be related to the expression of RANKL and OPG, to the RANKL/OPG ratio, to the angiogenic index, or to the number of myofibroblasts in these lesions.
authors.affiliation: Department of Oral Pathology, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Cassiano Francisco Weege
authors.initials: CF
authors.lastname: Nonaka
authors.forename: Roberta Barroso
authors.initials: RB
authors.lastname: Cavalcante
authors.forename: Renato Luiz Maia
authors.initials: RL
authors.lastname: Nogueira
authors.forename: Lélia Batista
authors.initials: LB
authors.lastname: de Souza
authors.forename: Leão Pereira
authors.initials: LP
authors.lastname: Pinto
mesh_terms: D018952:Antigens, CD34 / Q000032:analysis; D001478:Basal Cell Nevus Syndrome / Q000378:metabolism*; D001862:Bone Resorption / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D058628:Myofibroblasts / Q000378:metabolism*; D009389:Neovascularization, Pathologic; D009807:Odontogenic Cysts / Q000098:blood supply / Q000378:metabolism*; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*
chemical_list: D018952:Antigens, CD34; D053244:Osteoprotegerin; D053245:RANK Ligand