title: Comparative proteome analysis of Brucella abortus 2308 and its virB type IV secretion system mutant reveals new T4SS-related candidate proteins.
abstract: Brucella abortus is an alpha-2 proteobacteria with a type IV secretion system (T4SS) known as virB, which is necessary to gain virulence by building up a replicative vacuole associated with the endoplasmic reticulum of the host cell. A virB T4SS mutant of the B. abortus 2308 strain and its wild-type strain were grown in acid medium in order to obtain and analyze their proteomes, looking for putative proteins that may serve as T4SS substrates and those that may be subjected to T4SS regulation. A total of 47 overexpressed and 22 underexpressed proteins from the virB T4SS mutant strain were selected and sequenced. Some of the 69 analyzed proteins have not been described before either as over or under-expressed in relation to a virB T4SS mutation, whereas some of them have been already described by other groups as potentially important secretory proteins in other Brucella species. An important number of the proteins identified are outer membrane and periplasmic space protein, which makes them become particularly important new T4SS-related candidate proteins.
authors.affiliation: Departamentos de Inmunología y, Escuela Nacional de Ciencias Biológicas, IPN, México DF, Mexico.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Paredes-Cervantes
authors.forename: Raúl
authors.initials: R
authors.lastname: Flores-Mejía
authors.forename: Martha C
authors.initials: MC
authors.lastname: Moreno-Lafont
authors.forename: Humberto
authors.initials: H
authors.lastname: Lanz-Mendoza
authors.forename: Ángel T
authors.initials: ÁT
authors.lastname: Tello-López
authors.forename: Jane
authors.initials: J
authors.lastname: Castillo-Vera
authors.forename: Victoria
authors.initials: V
authors.lastname: Pando-Robles
authors.forename: Gerardo
authors.initials: G
authors.lastname: Hurtado-Sil
authors.forename: Edith
authors.initials: E
authors.lastname: González-González
authors.forename: Octavio
authors.initials: O
authors.lastname: Rodríguez-Cortés
authors.forename: Adriana
authors.initials: A
authors.lastname: Gutiérrez-Hoya
authors.forename: María T
authors.initials: MT
authors.lastname: Vega-Ramírez
authors.forename: Rubén
authors.initials: R
authors.lastname: López-Santiago
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D058947:Bacterial Secretion Systems; D002003:Brucella abortus / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation; D033903:Periplasmic Proteins / Q000096:biosynthesis* / Q000235:genetics; D020543:Proteome / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001425:Bacterial Outer Membrane Proteins; D058947:Bacterial Secretion Systems; D033903:Periplasmic Proteins; D020543:Proteome