title: In vivo evaluation of a new surfactant polymer coating mimicking the glycocalyx of endothelial cells.
abstract: The purpose of this study was to demonstrate that a proprietary surfactant polymer (SP) coating does not adversely affect the hemodynamic performance of cardiopulmonary bypass (CPB) or gas exchange in oxygenators. The new coating was applied to a CPB circuit including cannulae, reservoir, oxygenator, and blood pump implanted into 12 pigs, divided into groups with either coated or noncoated pumps. CPB flow was maintained at a fixed level of approximately 2.4 L/min for 6 hours with full heparinization. Hemodynamic data and pump performance were recorded every hour, and blood samples were taken every 2 hours. After sacrifice, the CPB circuit and major organs were macroscopically examined. There was no significant difference in the oxygen transfer rate between the two groups. The coating did not adversely affect oxygenator inlet or outlet pressures. There was no significant difference between the two groups in microthrombi seen in the oxygenators. No thromboemboli were noted in the major organs on gross or histologic examination. In conclusion, this new SP coating did not decrease gas exchange performance, and its biocompatibility evaluations revealed no differences between coated and noncoated groups under aggressive heparin use.
authors.affiliation: Department of Biomedical Engineering, Lerner Research Institute, the Cleveland Clinic, Cleveland, Ohio 44195, USA.
authors.forename: Akira
authors.initials: A
authors.lastname: Shiose
authors.forename: Tohru
authors.initials: T
authors.lastname: Takaseya
authors.forename: Hyun-Il
authors.initials: HI
authors.lastname: Kim
authors.forename: Mariko
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Horai
authors.forename: Santosh
authors.initials: S
authors.lastname: Rao
authors.forename: Yoko
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Masako
authors.initials: M
authors.lastname: Fujiki
authors.forename: Mark A
authors.initials: MA
authors.lastname: McCarthy
authors.forename: Yubiao
authors.initials: Y
authors.lastname: Liu
authors.forename: Jan J
authors.initials: JJ
authors.lastname: Lewandowski
authors.forename: Amy
authors.initials: A
authors.lastname: Davis
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Fukamachi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002315:Cardiopulmonary Bypass / Q000295:instrumentation*; D042783:Endothelial Cells / Q000166:cytology*; D005740:Gases; D019276:Glycocalyx / Q000378:metabolism*; D006439:Hemodynamics; D006493:Heparin / Q000737:chemistry; D008422:Materials Testing; D010100:Oxygen / Q000737:chemistry; D010106:Oxygenators; D010865:Pilot Projects; D011108:Polymers / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013552:Swine
chemical_list: D001672:Biocompatible Materials; D005740:Gases; D011108:Polymers; D013501:Surface-Active Agents; D006493:Heparin; D010100:Oxygen