title: Antibodies to HIV integrase catalyze site-specific degradation of their antigen.
abstract: HIV-1 integrase (IN) catalyzes integration of a DNA copy of the viral genome into the host genome. In contrast to canonical proteases (trypsin, chymotrypsin and proteinase K), IgGs and IgMs isolated from HIV-infected patients by affinity chromatography on immobilized IN specifically hydrolyzed only IN but not many other tested intact globular proteins. The sites of IN cleavage determined by MALDI mass spectrometry were localized mainly within seven known immunodominant regions of IN. Thin layer chromatography analysis has shown that the abzymes (Abzs) could also cleave 17 to 22-mer oligopeptides (OPs) corresponding to the immunodominant regions of IN sequence with a much higher rate than non-specific long peptides or three- and tetrapeptides of various sequence. Therefore, a prolonged incubation of IN with AIDS IgGs and IgMs having high catalytic activity usually produces many OPs of different length. Since anti-IN IgGs and IgMs can efficiently hydrolyze IN, a positive role of the Abzs in counteracting the infection is possible.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russia.
authors.forename: Elena S
authors.initials: ES
authors.lastname: Odintsova
authors.forename: Svetlana V
authors.initials: SV
authors.lastname: Baranova
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.forename: Valeriy A
authors.initials: VA
authors.lastname: Rasskazov
authors.forename: Christina
authors.initials: C
authors.lastname: Calmels
authors.forename: Vincent
authors.initials: V
authors.lastname: Parissi
authors.forename: Marie-Line
authors.initials: ML
authors.lastname: Andreola
authors.forename: Valentina N
authors.initials: VN
authors.lastname: Buneva
authors.forename: Olga D
authors.initials: OD
authors.lastname: Zakharova
authors.forename: Georgy A
authors.initials: GA
authors.lastname: Nevinsky
mesh_terms: D000293:Adolescent; D000328:Adult; D055162:Biocatalysis; D005260:Female; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology / Q000378:metabolism*; D015658:HIV Infections / Q000097:blood / Q000276:immunology; D019427:HIV Integrase / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D013379:Substrate Specificity; D055815:Young Adult
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019427:HIV Integrase