title: The influence of early-phase remodeling events on the biomechanical properties of engineered vascular tissues.
abstract: OBJECTIVE
  During the last decade, the use of ex vivo-derived materials designed as implant scaffolds has increased significantly. This is particularly so in the area of regenerative medicine, or tissue engineering, where the natural chemical and biomechanical properties have been shown to be advantageous. By focusing on detailed events that occur during early-phase remodeling processes, our objective was to detail progressive changes in graft biomechanics to further our understanding of these processes.
  
  
  METHODS
  A perfusion bioreactor system and acellular human umbilical veins were used as a model three-dimensional vascular scaffold on which human myofibroblasts were seeded and cultured under static or defined pulsatile conditions. Cell function in relation to graft mechanical properties was assessed.
  
  
  RESULTS
  Cells doubled in density from approximately 1 × 10(6) to 2 ± 0.4 × 10(6) cells/cm ringlet, whereas static cultures remained unchanged. The material's compressive stiffness and ultimate tensile strength remained unchanged in both static and dynamic systems. However the Young's modulus values increased significantly in the physiologic range, whereas in the failure range, a significant reduction (66%) was shown under dynamic conditions.
  
  
  CONCLUSIONS
  As pulse and flow conditions are modulated, complex mechanical changes are occurring that modify the elastic modulus differentially in both physiologic and failure ranges. Mechanical properties play an important role in graft patency, and a dynamic relationship between structure and function occurs during graft remodeling. These investigations have shown that as cells migrate into this ex vivo scaffold model, significant variation in material elasticity occurs that may have important implications in our understanding of early-stage vascular remodeling events.
authors.affiliation: J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL 32611-6131, USA.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Tosun
authors.forename: Carolina
authors.initials: C
authors.lastname: Villegas-Montoya
authors.forename: Peter S
authors.initials: PS
authors.lastname: McFetridge
mesh_terms: D001696:Biomechanical Phenomena; D001705:Bioprosthesis / Q000009:adverse effects; D019149:Bioreactors; D001807:Blood Vessel Prosthesis / Q000009:adverse effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D019245:Compressive Strength; D055119:Elastic Modulus; D006083:Graft Occlusion, Vascular / Q000209:etiology / Q000517:prevention & control; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology / Q000637:transplantation*; D006801:Humans; D058628:Myofibroblasts / Q000502:physiology / Q000637:transplantation*; D011474:Prosthesis Design; D011673:Pulsatile Flow; D013718:Tensile Strength; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014654:Vascular Patency