title: Aneuploidy rate in spermatozoa of selected men with severe teratozoospermia.
abstract: The aim of this study was to evaluate the incidence of spermatic aneuploidies in men with severe teratozoospermia and to determine an eventual relation between aneuploidies and a specific morphology of spermatozoa. Fluorescence in situ hybridisation (FISH) using a probe cocktail containing the alpha satellite for the centromeric region of chromosome X, Y and 18 was performed on decondensed spermatozoa from fresh ejaculates of thirty patients with severe teratozoospermia (abnormal forms >80%) and 15 fertile men with normal semen profiles. The mean frequency of teratozoospermia in patients was 91 ± 6.99%. There was statistically a significantly increased frequency of 1818, XY, XX and YY disomies in sperm with severe teratozoospermia compared with normal sperm (1.24% versus 0.08%, 1.42% versus 0.31%, 1.13% versus 0.19% and 1.11% versus 0.24%, respectively, P < 0.001 in all comparisons). The rate of total diploidy was significantly increased in patients compared with controls (1.46% versus 0.16%, P < 0.001). There was a correlation between macrocephalic spermatozoa and diploidy (r = 0.37, P < 0.05). Our data add further evidence that patients with severe teratozoospermia have an increased sperm aneuploidy rate and that this is particularly high in macrocephalic spermatozoa; FISH analysis on sperm could help to improve risk assessment and reproductive counselling in these individuals who are frequently candidates for intracytoplasmic sperm injection (ICSI) as a treatment of their infertility, as the use of ICSI has created consequential debate concerning the genetic risk for the offspring.
authors.affiliation: Department of Cytogenetics and Reproductive Biology, Farhat Hached University Teaching Hospital, Sousse, Tunisia. meriem.mehdi@rns.tn
authors.forename: M
authors.initials: M
authors.lastname: Mehdi
authors.forename: A
authors.initials: A
authors.lastname: Gmidène
authors.forename: S
authors.initials: S
authors.lastname: Brahem
authors.forename: J F
authors.initials: JF
authors.lastname: Guerin
authors.forename: H
authors.initials: H
authors.lastname: Elghezal
authors.forename: A
authors.initials: A
authors.lastname: Saad
mesh_terms: D000328:Adult; D000782:Aneuploidy; D016022:Case-Control Studies; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D013094:Spermatozoa / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes