title: New method for affinity adsorbent screening using gold colloids.
abstract: A rapid, effective method for the screening of adsorbent ligands based on the unique optical properties of gold colloid has been developed. Different interactions between adsorbate and ligands induce different states of aggregation of the gold colloid, and the associated distinct color changes of the colloid have been utilized to estimate the affinity of the ligands toward the adsorbate. In this work, phosphorylated peptide CGGFGGpSG was appended to a gold colloid to obtain the adsorbate-modified gold colloid (CG8-AuNPs). Candidate ligands Dpa-Zn(2+), DMAPAA, and AAn were copolymerized with acrylamide to form linear polymers and cross-linked CG8-AuNPs to induce aggregation. Screening of the candidate ligands revealed that Dpa-Zn(2+) showed the highest affinity among those tested, inducing a color change of the gold colloid at a concentration of 10 μM, which is much lower than those of ligands DMAPAA (40 μM) and AAn (almost no color change could be observed). Subsequent statistical adsorption experiments confirmed these screening results, with the adsorbent A-AAm-Dpa-Zn(2+) showing the highest adsorption capacity (426 mg/g) for CG-8, almost twice that of adsorbent A-AAm-DMAPAA. This reported method has low sample consumption, and the screening may be simply monitored by the naked eye.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University, Tianjin 300071, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qinqin
authors.initials: Q
authors.lastname: Wei
authors.forename: Saihui
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D003102:Colloids; D006046:Gold / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D003102:Colloids; D055767:Immobilized Proteins; D008024:Ligands; D009842:Oligopeptides; D006046:Gold