title: Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.
abstract: Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution.
authors.affiliation: Department of Tissue Engineering, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic. prosecka@biomed.cas.cz
authors.forename: E
authors.initials: E
authors.lastname: Prosecká
authors.forename: M
authors.initials: M
authors.lastname: Rampichová
authors.forename: L
authors.initials: L
authors.lastname: Vojtová
authors.forename: D
authors.initials: D
authors.lastname: Tvrdík
authors.forename: S
authors.initials: S
authors.lastname: Melčáková
authors.forename: J
authors.initials: J
authors.lastname: Juhasová
authors.forename: M
authors.initials: M
authors.lastname: Plencner
authors.forename: R
authors.initials: R
authors.lastname: Jakubová
authors.forename: J
authors.initials: J
authors.lastname: Jančář
authors.forename: A
authors.initials: A
authors.lastname: Nečas
authors.forename: P
authors.initials: P
authors.lastname: Kochová
authors.forename: J
authors.initials: J
authors.lastname: Klepáček
authors.forename: Z
authors.initials: Z
authors.lastname: Tonar
authors.forename: E
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D006882:Hydroxyapatites; D003094:Collagen