title: JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
abstract: In adult mammals, hematopoietic stem cells (HSCs) reside in the bone marrow (BM) and are maintained in a quiescent and undifferentiated state through adhesive interactions with specialized microenvironmental niches. Although junctional adhesion molecule-C (JAM-C) is expressed by HSCs, its function in adult hematopoiesis remains elusive. Here, we show that HSCs adhere to JAM-B expressed by BM stromal cells in a JAM-C dependent manner. The interaction regulates the interplay between HSCs and BM stromal cells as illustrated by the decreased pool of quiescent HSCs observed in jam-b deficient mice. We further show that this is probably because of alterations of BM stromal compartments and changes in SDF-1α BM content in jam-b(-/-) mice, suggesting that JAM-B is an active player in the maintenance of the BM stromal microenvironment.
authors.affiliation: Centre de Recherche en Cancérologie de Marseille, Unité Mixle de Recherche, Marseille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Arcangeli
authors.forename: Vincent
authors.initials: V
authors.lastname: Frontera
authors.forename: Florence
authors.initials: F
authors.lastname: Bardin
authors.forename: Elodie
authors.initials: E
authors.lastname: Obrados
authors.forename: Susanne
authors.initials: S
authors.lastname: Adams
authors.forename: Christian
authors.initials: C
authors.lastname: Chabannon
authors.forename: Claudine
authors.initials: C
authors.lastname: Schiff
authors.forename: Stephane J C
authors.initials: SJ
authors.lastname: Mancini
authors.forename: Ralf H
authors.initials: RH
authors.lastname: Adams
authors.forename: Michel
authors.initials: M
authors.lastname: Aurrand-Lions
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D049109:Cell Proliferation; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000378:metabolism / Q000502:physiology*; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D062765:Junctional Adhesion Molecule B
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C498781:Jam2 protein, mouse; D062765:Junctional Adhesion Molecule B; C486723:Jam3 protein, mouse