title: Colorimetric and luminescent bifunctional Ru(II) complex-modified gold nano probe for sensing of DNA.
abstract: A colorimetric and luminescent bifunctional Ru(II) complex-modified gold nano-probe for sensing ctDNA was developed. A new water-soluble Ru(II) complex containing an aromatic α-diimine ligand with an extended π system was found to be emissive at about 588nm in water and the emission intensity of the complex was enhanced about 3.4-fold upon addition of calf thymus DNA (ctDNA) in aqueous buffer with no color changes. The detection limit was 70nM with a 1.2% relative standard deviation (RSD, n=5). The Ru(II) complex chromophore was used to modify 8nm MHA carboxylate-functionalized gold nanoparticles to produce a colorimetric and luminescent bifunctional probe for sensing ctDNA in aqueous buffer at room temperature. The obvious change in visible color and enhancement of emission intensity of the functionalized gold-Ru(II) complex colloids upon addition of ctDNA was due to the electron or energy transfer between Ru(II) chromophore and the Au-NPs. The limit of detection was 1.0nM for ctDNA with a 4.5% RSD (n=5). With such a high sensitivity, the bifunctional Ru(II) complex-modified gold nano-probe will be potentially suitable for the DNA sensing in bioanalytical application.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis & Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, China. mjli@fzu.edu.cn
authors.forename: Mei-Jin
authors.initials: MJ
authors.lastname: Li
authors.forename: Mei-Juan
authors.initials: MJ
authors.lastname: Nie
authors.forename: Zhao-Zhen
authors.initials: ZZ
authors.lastname: Wu
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Guo-Nan
authors.initials: GN
authors.lastname: Chen
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D006046:Gold; D049449:Luminescence; D053768:Metal Nanoparticles; D009942:Organometallic Compounds; D012428:Ruthenium
chemical_list: D009942:Organometallic Compounds; D006046:Gold; D012428:Ruthenium; D004247:DNA