title: Structure and genetics of the O-antigen of Escherichia coli O158.
abstract: A structure of the O-polysaccharide (O-antigen) of Escherichia coli O158 has been reported (Datta, A. K.; Basu, S.; Roy, N. Carbohydr. Res.1999, 322, 219-227). In this work, we reinvestigated the O158 polysaccharide using sugar analyses, Smith degradation, and (1)H and (13)C NMR spectroscopy and established the following structure, which is at variance with the structure established earlier: [see formula in text]. This structure is in agreement with the predicted functions of genes found in the O-antigen gene cluster of E. coli O158.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
authors.forename: Andrei V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Liuqing
authors.initials: L
authors.lastname: Wen
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D019081:O Antigens