title: Vestibules are part of the substrate path in the multidrug efflux transporter AcrB of Escherichia coli.
abstract: The path of substrates in the multidrug efflux pump AcrB of Escherichia coli was examined by using labeling with a lipophilic substrate mimic, Bodipy FL maleimide. Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrate transport, whereas only one (out of nine) residue in the central cavity tested positive.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202, USA.
authors.forename: Fasahath
authors.initials: F
authors.lastname: Husain
authors.forename: Mihir
authors.initials: M
authors.lastname: Bikhchandani
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nikaido
mesh_terms: D020816:Amino Acid Motifs; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001665:Binding Sites; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008968:Molecular Conformation; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; C083496:AcrB protein, E coli