title: Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes.
abstract: BACKGROUND
  The aim of this study is to investigate the effect of quercetin in alleviating the cytotoxic effects of Dimethoate in human peripheral blood lymphocytes.
  
  
  METHODS
  Lymphocytes were divided into too groups. The first group, lymphocytes were incubated for 4 h at 37°C with different concentrations (0, 40, 60 and 100 mM) of Dimethoate. The second group was preincubated with quercetin for 30 min and followed by Dim incubation for 4 h at 37°C.
  
  
  RESULTS
  Following in vitro incubation, Dimethoate caused a significant increase in malondialdehyde levels, a significant decrease in thiol levels, as well as a significant increase in superoxide dismutase, and catalase activities in lymphocytes at different concentrations. Quercetin pretreated lymphocytes showed a significant protection against the cytotoxic effects inducted by Dimethoate on the studied parameters.
  
  
  CONCLUSIONS
  In conclusion, antioxidant quercetin could protect against Dimethoate-induced oxidative stress by decreasing lipid peroxidation, protein oxidation and increasing superoxide dismutase and catalase activities in human lymphocytes.
authors.affiliation: Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, 3038 Tunisie. bochragargouri@yahoo.fr
authors.forename: Bochra
authors.initials: B
authors.lastname: Gargouri
authors.forename: Riadh Ben
authors.initials: RB
authors.lastname: Mansour
authors.forename: Fatma Ben
authors.initials: FB
authors.lastname: Abdallah
authors.forename: Abdelfetteh
authors.initials: A
authors.lastname: Elfekih
authors.forename: Saloua
authors.initials: S
authors.lastname: Lassoued
authors.forename: Hamden
authors.initials: H
authors.lastname: Khaled
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004117:Dimethoate / Q000633:toxicity*; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D011506:Proteins / Q000737:chemistry; D011794:Quercetin / Q000494:pharmacology*; D013439:Sulfhydryl Reagents / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D007306:Insecticides; D011506:Proteins; D013439:Sulfhydryl Reagents; D008315:Malondialdehyde; D004228:Dithionitrobenzoic Acid; D011794:Quercetin; D002374:Catalase; D013482:Superoxide Dismutase; D004117:Dimethoate