title: Efficiency of Plasmocin™ on various mammalian cell lines infected by mollicutes in comparison with commonly used antibiotics in cell culture: a local experience.
abstract: Mycoplasma contamination is a deleterious event for cell culture laboratories. Plasmocin™ is used to prevent and eradicate mycoplasma infections from cell. In this study, 80 different mammalian cell lines from various sources; human, monkey, mice, hamster and rat were used to study and evaluate plasmocin™ efficiency and compare it to commonly used antibiotics such as BM-cyclin, ciprofloxacin and mycoplasma removal agent (MRA). It was shown that mycoplasma infections were eradicated by plasmocin™, BM-cyclin, ciprofloxacin and MRA in 65%, 66.25%, 20%, and 31.25%, respectively, of infected cell cultures. However, re-infection with mycoplasmas after the period of 4 months occurred in 10-80% of the studied cell lines. Cell cytotoxicity and culture death was observed in 25, 17.5 and 10% of the treated cells, for plasmocin™, BM-cyclin and MRA, respectively. In this study, Plasmocin™ showed strong ability to eradicate mollicutes from our cell lines with minimal percentage of regrowth. However, due to its high cell cytotoxicity it should be used with caution especially when dealing with expensive or hard-to-obtain cell lines. Amongst the antibiotics tested, BM-cyclin was shown to remove mycoplasma with the highest efficiency.
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Molla Kazemiha
authors.forename: Shahram
authors.initials: S
authors.lastname: Azari
authors.forename: Amir
authors.initials: A
authors.lastname: Amanzadeh
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
authors.forename: Morteza
authors.initials: M
authors.lastname: Shojaei Moghadam
authors.forename: Mahdi
authors.initials: M
authors.lastname: Habibi Anbouhi
authors.forename: Susan
authors.initials: S
authors.lastname: Maleki
authors.forename: Nahid
authors.initials: N
authors.lastname: Ahmadi
authors.forename: Tahmineh
authors.initials: T
authors.lastname: Mousavi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar