title: Enzyme-linked immunosorbent assay and polymerase chain reaction performance using Mexican and Guatemalan discrete typing unit I strains of Trypanosoma cruzi.
abstract: Thirteen Trypanosoma cruzi isolates from different geographic regions of Mexico and Guatemala belonging to discrete typing unit (DTU) I and a reference CL-Brener (DTU VI) strain were used to perform enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). A panel of 57 Mexican serum samples of patients with chronic chagasic cardiopathy and asymptomatic infected subjects (blood bank donors) were used in this study. DNA from the above 14 T. cruzi strains were extracted and analyzed by PCR using different sets of primers designed from minicircle and satellite T. cruzi DNA. The chronic chagasic cardiopathy serum samples were easily recognized with ELISA regardless of the source of antigenic extract used, even with the CL-Brener TcVI, but positive serum samples from blood bank donors in some cases were not recognized by some Mexican antigenic extracts. On the other hand, PCR showed an excellent performance despite the set of primers used, since all Mexican and Guatemalan T. cruzi strains were correctly amplified. In general terms, Mexican, Guatemalan, and CL-Brener T. cruzi strains are equally good sources of antigen when using the ELISA test to detect Mexican serum samples. However, there are some strains with poor performance. The DTU I strains are easily detected using either kinetoplast or satellite DNA target designed from DTU VI strains.
authors.affiliation: Instituto Nacional Cardiologia I. Chávez, Mexico DF, Mexico.
authors.forename: Martha
authors.initials: M
authors.lastname: Ballinas-Verdugo
authors.forename: Pedro Antonio
authors.initials: PA
authors.lastname: Reyes
authors.forename: Ana
authors.initials: A
authors.lastname: Mejia-Dominguez
authors.forename: Ruth
authors.initials: R
authors.lastname: López
authors.forename: Vivian
authors.initials: V
authors.lastname: Matta
authors.forename: Victor M
authors.initials: VM
authors.lastname: Monteón
mesh_terms: D000953:Antigens, Protozoan / Q000097:blood*; D001782:Blood Donors; D002598:Chagas Cardiomyopathy / Q000097:blood / Q000175:diagnosis*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards*; D006166:Guatemala; D006801:Humans; D008800:Mexico; D016133:Polymerase Chain Reaction / Q000592:standards*; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000953:Antigens, Protozoan; D017931:DNA Primers; D016054:DNA, Protozoan