title: [Alteration of brown adipocyte Ca2+ responses in culture by adrenergic activation].
abstract: Thermogenic capability of brown adipose tissue is controlled by norepinephrine. Interaction of norepinephrine with adipocyte at- and P3-adrenergic receptors results in the increase of Ca2+ and cAMP concentrations. The [Ca2+]i changes initiated by norepinephrine and selective agonists of alpha1- and beta-adrenergic receptors, cirazolin and isoproterenol, were recorded in single cells of primary culture on the 1st, 3rd and 6th days in vitro. On the first day, isoproterenol-induced [Ca2+]i changes as compared to cirazolin-induced ones were characterized by greater amplitude and lesser impulse duration over the entire range of physiological concentrations used. These differences were negligible after 3 days and kinetic differences were practically absent after 6 days of cultivation. The agonist-induced [Ca2+]i changes in proliferating and differentiated cells differed significantly: in the process of cell growth in culture, the amplitude of calcium response increased, the duration of impulse signal decreased and the sensitivity to adrenergic agonists increased. The Ca2+ store in endoplasmic reticulum increased during the cell growth and development in culture, according to thapsigargin-induced Ca2+ response amplitude increase in Ca2+ free medium. The rate of Ca2+ pumping out of cell characterizing PMCA-activity also increased.
authors.forename: E A
authors.initials: EA
authors.lastname: Turovskiĭ
authors.forename: M V
authors.initials: MV
authors.lastname: Konakov
authors.forename: A V
authors.initials: AV
authors.lastname: Berezhnov
authors.forename: V P
authors.initials: VP
authors.lastname: Zinchenko
authors.forename: G E
authors.initials: GE
authors.lastname: Bronnikov
authors.forename: L P
authors.initials: LP
authors.lastname: Dolgacheva
mesh_terms: D052437:Adipocytes, Brown / Q000187:drug effects*; D002001:Adipose Tissue, Brown / Q000187:drug effects; D000322:Adrenergic Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D000322:Adrenergic Agonists; D000319:Adrenergic beta-Antagonists; D004791:Enzyme Inhibitors; D007093:Imidazoles; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D019284:Thapsigargin; D011433:Propranolol; D007545:Isoproterenol; C014282:cirazoline; D002118:Calcium; D009638:Norepinephrine