title: Effects of the Hb E, Hb H and Hb G-Taichung variants on HbA1c values by the Bio-Rad variant II turbo analyzer.
abstract: OBJECTIVE
  The influences of Hb variants on HbA(1c) values can cause mismanagement of diabetes; therefore, the effects of Hb E, H, and G-Taichung variants were evaluated.
  
  
  METHODS
  HbA(1c) values of 2105 samples, including 37 samples with Hb E, H and G-Taichung variants identified by Hb electrophoresis and the PCR sequence, were evaluated by the ion-exchange (Bio-Rad Variant II Turbo analyzer) and boronate affinity (Primus CLC 385 analyzer) high performance liquid chromatography (HPLC) methods.
  
  
  RESULTS
  In the patients with the Hb E and H variants, their HbA(1c) values determined by ion-exchange HPLC were significantly higher than those by boronate affinity HPLC. However, there were no significant differences of the HbA(1c) values in the patients with the Hb G-Taichung variant.
  
  
  CONCLUSIONS
  The HbA1c levels might be interfered by the Hb E and H variants, but not the Hb G-Taichung variant, measured by the Bio-Rad Variant II Turbo analyzer.
authors.affiliation: Department of Laboratory Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Su-Chen
authors.initials: SC
authors.lastname: Lee
authors.forename: Li-Hsuan
authors.initials: LH
authors.lastname: Wang
authors.forename: Shih-Meng
authors.initials: SM
authors.lastname: Tsai
authors.forename: Hung-Yao
authors.initials: HY
authors.lastname: Fang
authors.forename: Li-Yu
authors.initials: LY
authors.lastname: Tsai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood* / Q000235:genetics*; D005260:Female; D014644:Genetic Variation; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D006446:Hemoglobin E / Q000032:analysis / Q000235:genetics*; D006447:Hemoglobin H / Q000032:analysis / Q000235:genetics*; D006455:Hemoglobins, Abnormal / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008297:Male
chemical_list: D006442:Glycated Hemoglobin; D006446:Hemoglobin E; D006447:Hemoglobin H; D006455:Hemoglobins, Abnormal; C517652:hemoglobin A1c protein, human; C043216:hemoglobin Chinese