title: [Mechanism of malate dehydrogenase isoform formation in Sphaerotilus natans D-507 under different cultivation conditions].
abstract: Electrophoretically homogenous preparations of malate dehydrogenase (MDH) isoforms of the bacteria Sphaerotilus natans D-507 with specific activity 7.46 U/mg and 5.74 U/mg with respect to protein concentration have been obtained. The dimeric isoform of the enzyme was shown to function under organotrophic growth conditions, whereas the tetrameric isoform was induced under mixotrophic cultivation conditions. PCR-analysis revealed a single gene encoding the malate dehydrogenase molecule. The topography of the MDH isoform surface was studied by atomic-force microscopy, and a 3D-structure of the enzyme was obtained. Spectraphotometric analysis data allowed us to suggest that stabilization of the tetrameric form of MDH is due to additional bounds implicated in the quaternary structure formation.
authors.forename: A T
authors.initials: AT
authors.lastname: Eprintsev
authors.forename: M I
authors.initials: MI
authors.lastname: Falaleeva
authors.forename: M A
authors.initials: MA
authors.lastname: Arabtseva
authors.forename: I A
authors.initials: IA
authors.lastname: Lavrinenko
authors.forename: I V
authors.initials: IV
authors.lastname: Parfenova
authors.forename: M V
authors.initials: MV
authors.lastname: Grechkina
authors.forename: F S
authors.initials: FS
authors.lastname: Abud
mesh_terms: D018929:Cell Culture Techniques; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000302:isolation & purification; D020033:Protein Isoforms / Q000737:chemistry* / Q000302:isolation & purification; D020836:Protein Structure, Quaternary; D042744:Sphaerotilus / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D020033:Protein Isoforms; D008291:Malate Dehydrogenase