title: [The establishment of real time fluorescent PCR detecting method and preliminary application on pathogenesis of HFMD].
abstract: OBJECTIVE
  To establish the real-time fluorescent PCR method for detecting enterovirus, enterovirus 71 and Coxsackievirus A 16 nucleic acid.
  
  
  METHODS
  Primers and MGB probe were chosen for virus gene. The samples of 38 HFMD patients were analyzed by TaqMan-MGB PCR technique on a fluorescence real-time PCR instrument,and the results were compared with those by conventional RT-PCR.
  
  
  RESULTS
  The real-time fluorescent PCR positive rates of EV, EV71 and Cox A16 were 73.7%, 60.5%, 13.2%; the conventional RT-PCR were 71.1%, 55.3%, 13.2%. There were no significant differences between the two methods.
  
  
  CONCLUSIONS
  The real-time fluorescent PCR detecting method of EV, EV71 and Cox A16 nucleic acid have been established successfully.
authors.affiliation: Military Postgraduate Medical College, Beijing 100853, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tang
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Sheng
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Chen
authors.forename: Bo-an
authors.initials: BA
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
mesh_terms: D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D029821:Enterovirus A, Human / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006232:Hand, Foot and Mouth Disease / Q000175:diagnosis* / Q000473:pathology / Q000821:virology; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes