title: Assessment of endothelial permeability and leukocyte transmigration in human endothelial cell monolayers.
abstract: Increased vascular permeability is the hallmark of inflammation. Here, we describe three methods to assess vascular permeability in cell culture: (1) Impedance measurements of endothelial cell monolayers that allow to monitor changes in cell shape in real time. (2) Diffusion of fluorescently labeled dextran across endothelial cell monolayers to identify openings large enough for bulky molecules. (3) Transmigration of neutrophils through confluent endothelial cell monolayers to study one major process that increases endothelial permeability in inflammation.
authors.affiliation: Institute of Pharmacology and Toxicology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Ludwig
authors.forename: Anselm
authors.initials: A
authors.lastname: Sommer
authors.forename: Stefan
authors.initials: S
authors.lastname: Uhlig
mesh_terms: D002199:Capillary Permeability / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D017097:Electric Impedance; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007365:Intercellular Junctions / Q000187:drug effects; D016209:Interleukin-8 / Q000276:immunology / Q000494:pharmacology; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D018613:Microscopy, Confocal; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D011199:Potentiometry / Q000379:methods*; D013917:Thrombin / Q000494:pharmacology
chemical_list: D003911:Dextrans; D016209:Interleukin-8; D013917:Thrombin