title: Identification of CM1 as a Pathogenic Factor in Inflammatory Diseases and Cancer.
abstract: BACKGROUND
  CM1 (centrocyte/-blast marker 1) was defined by a mAb against concanavalin A (Con A) activated PBMC. It is expressed in germinal center of human tonsil and on the surface of activated PBMC as well as cancer cells. Recently, increased productions of pro-inflammatory mediators were detected from activated PBMC by CM1 ligation.
  
  
  METHODS
  However, there is a limitation to explain the exact role of CM1 on inflammation and its related mechanisms, since the identity of CM1 is still not clarified. In our previous study, we have already confirmed that soluble form of CM1 was produced by Raji. Therefore, we performed Q-TOF analysis after immunoprecipitation of concentrated Raji culture supernatant using anti-CM1 mAbs.
  
  
  RESULTS
  As a result, we found that CM1 is identical to enolase-1(ENO1), a glycolytic enzyme, and we confirmed that results by silencing ENO1 using siRNA. It was also confirmed through competition assay between anti-CM1 and anti-ENO1 mAbs. Finally, we investigated the possible role of CM1 in inflammatory response and cancer. The ligation of CM1 on Raji cells with anti-CM1 mAbs induces the extensive production of prostaglandin E(2)(PGE(2)). In addition, the increased activity of matrix metalloproteinase (MMP)-2/9 was shown in NCI-N87, stomach cancer cell line by CM1 stimulation.
  
  
  CONCLUSIONS
  CM1 is identical to ENO1 and it might be an important role in the regulation of inflammatory responses.
authors.affiliation: Department of Anatomy and Tumor Immunity Medical Research Center, Seoul National University College of Medicine, Seoul 110-799, Korea.
authors.forename: Seyeon
authors.initials: S
authors.lastname: Bae
authors.forename: Hyemin
authors.initials: H
authors.lastname: Kim
authors.forename: Yeon Sil
authors.initials: YS
authors.lastname: Yu
authors.forename: Na-Eun
authors.initials: NE
authors.lastname: Lee
authors.forename: Joo Myoung
authors.initials: JM
authors.lastname: Kong
authors.forename: Hang-Rae
authors.initials: HR
authors.lastname: Kim
authors.forename: Young-Il
authors.initials: YI
authors.lastname: Hwang
authors.forename: Yeong Wook
authors.initials: YW
authors.lastname: Song
authors.forename: Jae Seung
authors.initials: JS
authors.lastname: Kang
authors.forename: Wang Jae
authors.initials: WJ
authors.lastname: Lee