title: Characterization and diagnostic use of a monoclonal antibody for VP28 envelope protein of white spot syndrome virus.
abstract: The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV) was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21. After induction, the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal antibody (MAb) production. It was observed by immuno-electron microscopy the MAbs specific to rVP28 could recognize native VP28 target epitopes of WSSV and dot-blot analysis was used to detect natural WSSV infection. Competitive PCR showed that the viral level was approximately 10(4) copies/mg tissue in the dilution of gill homogenate of WSSV-infected crayfish at the detection limit of dot-blot assay. Our results suggest that dot-blot analysis with anti-rVP28 MAb could rapidly and sensitively detect WSSV at the early stages of WSSV infection.
authors.affiliation: Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, China.
authors.forename: Chong-lin
authors.initials: CL
authors.lastname: Hou
authors.forename: Yu
authors.initials: Y
authors.lastname: Cao
authors.forename: Rong-hui
authors.initials: RH
authors.lastname: Xie
authors.forename: Yi-zhen
authors.initials: YZ
authors.lastname: Wang
authors.forename: Hua-hua
authors.initials: HH
authors.lastname: Du
mesh_terms: D000911:Antibodies, Monoclonal; D004926:Escherichia coli / Q000821:virology; D015151:Immunoblotting; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; C548473:VP28 protein, white spot syndrome virus; D014759:Viral Envelope Proteins