title: Rational design of a cyclin A fluorescent peptide sensor.
abstract: We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.
authors.affiliation: Departamento de Química Orgánica y Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Unidad Asociada al CSIC, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pazos
authors.forename: Miguel
authors.initials: M
authors.lastname: Pérez
authors.forename: Hugo
authors.initials: H
authors.lastname: Gutiérrez-de-Terán
authors.forename: Mar
authors.initials: M
authors.lastname: Orzáez
authors.forename: Tatiana
authors.initials: T
authors.lastname: Guevara
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
mesh_terms: D001665:Binding Sites; D001681:Biological Assay; D015374:Biosensing Techniques / Q000379:methods*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019925:Cyclin A / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D054802:Molecular Imprinting / Q000379:methods*; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D010797:Phthalimides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D047428:Protein Kinase Inhibitors / Q000378:metabolism / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D019925:Cyclin A; D005456:Fluorescent Dyes; D019151:Peptide Library; D010455:Peptides; D010797:Phthalimides; D047428:Protein Kinase Inhibitors; D018844:Cyclin-Dependent Kinases