title: Mass-spectrometric characterization of peroxidized and hydrolyzed lipids in plasma and dendritic cells of tumor-bearing animals.
abstract: Dendritic cells are the most potent antigen presenting cells responsible for the development of immune responses in cancer. However, it is known that the function of dendritic cells in tumor-bearing hosts is severely compromised. Our previous studies demonstrated that the defects in dendritic cell function are due, to a large extent, to the accumulation of high amounts of lipids--predominantly triglycerides--in a substantial proportion of dendritic cells in tumor-bearing mice and patients with cancer. The dendritic cells accumulation of lipids is likely associated with their up-regulation of a scavenger receptor A. This receptor is primarily responsible for uptake of modified lipids. Here, by using different versions of liquid chromatography-mass spectrometry, we identified several molecular species of oxygenated lipids in plasma of tumor-bearing animals that may be responsible for their uptake and accumulation by dendritic cells via scavenger receptor A-dependent pathway--the effect that may be associated with the loss of dendritic cell's immune surveillance function in cancer.
authors.affiliation: Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA 15219-3130, USA.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Tyurin
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Yulia Y
authors.initials: YY
authors.lastname: Tyurina
authors.forename: Dmitry I
authors.initials: DI
authors.lastname: Gabrilovich
authors.forename: Valerian E
authors.initials: VE
authors.lastname: Kagan
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003713:Dendritic Cells / Q000276:immunology*; D006868:Hydrolysis; D007157:Immunologic Surveillance; D015227:Lipid Peroxidation; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000097:blood / Q000276:immunology*; D051117:Scavenger Receptors, Class A / Q000235:genetics; D014280:Triglycerides / Q000097:blood / Q000737:chemistry* / Q000378:metabolism
chemical_list: C495140:Msr1 protein, mouse; D051117:Scavenger Receptors, Class A; D014280:Triglycerides