title: [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].
abstract: OBJECTIVE
  We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.
  
  
  METHODS
  By using dilution-plate method we isolated dominant bacteria, dominant fungi and actinomycetes from the rhizosphere of continuous cropping with peanut. Morphological specificity, culture shape, physiological-biochemical characteristic and partial 16S rDNA sequences were used to identify bacteria and actinomycetes. Morpholog, growth on various media, and Internal Transcribed Spacer (ITS) rDNA sequences homology analysis were performed to identify dominant fungi.
  
  
  RESULTS
  We isolated seven dominant bacteria strains, seven dominant fungi and seven dominant actinomycetes. Dominant bacteria were identified as Leifsonia xyli, Arthrobacter chlorophenolicus, Microbacterium flavescens, Sphingomonas sp., Pasteurella sp., Bacillus simplex and Bacillus megaterium. Dominant fungi were identified as Cladosporium cladosporioides, Penicillium purpurogenum , Hypocrea lixii, Exophiala pisciphila, Penicillium janthinellum, Aspergillus sp. and Verticillium dahliae. Dominant actinomycetes were identified as Streptomyces violaceoruber, Streptomyces flaveus, Streptomyces panaciterrae, Streptomyces achromogenes, Streptomyces pseudogriseolus, Streptomyces cellulosae and Streptomyces aureus.
  
  
  CONCLUSIONS
  This study was the first time to isolate and identify dominant microorganisms from the rhizosphere of continuous cropping with peanut. The type of dominant microorganisms changed obviously after planting peanut, although the change was without regularity.
authors.affiliation: College of Life Science, Qingdao Agricultural University, Qingdao 266109, China. yywttl@126.com
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Yan
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Hongquan
authors.initials: H
authors.lastname: Xian
authors.forename: Dejie
authors.initials: D
authors.lastname: Cui
mesh_terms: D010367:Arachis / Q000382:microbiology*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D058441:Rhizosphere; D012988:Soil Microbiology