title: Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora.
abstract: A Micromonospora-like strain, RtIII47(T), was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013(T) (98.0%). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868(T) (97.9%), Plantactinospora mayteni YIM 61359(T) (97.9%), Salinispora tropica CNB-440(T) (97.8%), Micromonospora peucetia DSM 43363(T) (97.7%), Micromonospora auratinigra TT1-11(T) (97.7%), Verrucosispora sediminis CGMCC 4.3550(T) (97.6%) and Salinispora arenicola CNH-643(T) (97.5%). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47(T) should be assigned to the genus Verrucosispora. DNA-DNA relatedness between strain RtIII47(T) and the most closely related type strain, V. lutea YIM 013(T), was less than 40%. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora, and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47(T) (=CGMCC 4.5826(T) =NBRC 106684(T)) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Xi
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Ruan
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002241:Carbohydrates / Q000032:analysis; D002681:China; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D008848:Micromonosporaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D053833:Wetlands
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase