title: Interleukin-33 levels in gingival crevicular fluid, saliva, or plasma do not differentiate chronic periodontitis.
abstract: BACKGROUND
  This study investigates whether gingival crevicular fluid (GCF), saliva, and plasma levels of interleukin-33 (IL-33) can differentiate individuals with chronic periodontitis from individuals with healthy periodontium.
  
  
  METHODS
  GCF, whole saliva, and plasma samples together with full-mouth clinical periodontal recordings were obtained from 32 otherwise healthy, non-smoker chronic periodontitis individuals and 25 systemically and periodontally healthy, non-smoker individuals. IL-33 levels in the biofluid samples were determined by enzyme-linked immunosorbent assay. Data were tested statistically by Mann-Whitney U test.
  
  
  RESULTS
  The GCF concentrations of IL-33 were significantly lower in chronic periodontitis individuals than in healthy individuals (P <0.0001), whereas the total amounts in GCF samples were similar (P >0.05). The salivary and plasma contrations of IL-33 were indifferent in the two study groups (P >0.05).
  
  
  CONCLUSIONS
  According to the present findings, the GCF, saliva or plasma levels of IL-33 could not differentiate chronic periodontitis individuals and periodontally healthy individuals. Larger-scale intervention studies may better clarify this issue.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey. nurcan.buduneli@ege.edu.tr
authors.forename: Nurcan
authors.initials: N
authors.lastname: Buduneli
authors.forename: Özgün
authors.initials: Ö
authors.lastname: Özçaka
authors.forename: Ayşe
authors.initials: A
authors.lastname: Nalbantsoy
mesh_terms: D000328:Adult; D000368:Aged; D016301:Alveolar Bone Loss / Q000145:classification / Q000276:immunology; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D055113:Chronic Periodontitis / Q000097:blood / Q000145:classification / Q000276:immunology*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D005884:Gingival Hemorrhage / Q000145:classification / Q000276:immunology; D006801:Humans; D000067596:Interleukin-33; D007378:Interleukins / Q000032:analysis* / Q000097:blood; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification / Q000276:immunology; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification / Q000276:immunology; D010519:Periodontium / Q000378:metabolism; D016300:Radiography, Bitewing; D012463:Saliva / Q000737:chemistry*
chemical_list: D015415:Biomarkers; C506536:IL33 protein, human; D000067596:Interleukin-33; D007378:Interleukins