title: [Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw].
abstract: In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 degrees C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L x h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 degrees C-50 degrees C.
authors.affiliation: College of Life Science, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Ren
authors.forename: Keke
authors.initials: K
authors.lastname: Cheng
authors.forename: Andong
authors.initials: A
authors.lastname: Song
authors.forename: Jian'an
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002384:Catalysis; D002480:Cellulase / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D018547:Plant Stems; D013227:Steam; D003313:Zea mays
chemical_list: D013227:Steam; D002480:Cellulase