title: Role of nitric oxide (NO) metabolism and inflammatory mediators in childhood obesity.
abstract: OBJECTIVE
  The role of NO and adipocytokines in childhood obesity was studied, supposing that obesity provokes inflammation. Children were admitted to the pediatric clinic for a regular check up because of obesity.
  
  
  METHODS
  Obese (n = 79) and healthy (n = 12) children were selected and divided into subgroups according to their age, gender, glucose tolerance and nitric oxide synthase (NOS II) positivity.
  
  
  METHODS
  Urine and blood nitrite plus nitrate, the expression of NOS II in white blood cells, serum adipocytokines and clinical characteristics were analyzed in each group. Significance was tested by unpaired two-tailed t test and by ANOVA.
  
  
  RESULTS
  NOS II was only detected in the white blood cells of a subgroup (17/79) of obese children. Serum leptin and resistin concentrations were significantly higher, adiponectin was lower compared to healthy children. Significant correlations were observed between serum adiponectin and resistin levels (reciprocal, R (2) = 0.4), and between body mass index and serum leptin levels.
  
  
  CONCLUSIONS
  NOS II expression in white blood cells was observed in a minority of patients. Low-grade inflammation in obese children was suggested by the increased resistin levels, particularly in NOS II-positive patients. Correlation between different adipocytokines was restricted for a few subgroups.
authors.affiliation: Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47., POB 260, Budapest, 1444, Hungary. andras.hrabak@eok.sote.hu
authors.forename: András
authors.initials: A
authors.lastname: Hrabák
authors.forename: László
authors.initials: L
authors.lastname: Derzbach
authors.forename: Ildikó
authors.initials: I
authors.lastname: Csuka
authors.forename: Tamás
authors.initials: T
authors.lastname: Bajor
authors.forename: Anna
authors.initials: A
authors.lastname: Körner
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D054392:Adipokines / Q000378:metabolism; D052242:Adiponectin / Q000096:biosynthesis; D000293:Adolescent; D015992:Body Mass Index; D002097:C-Reactive Protein / Q000096:biosynthesis; D002648:Child; D002675:Child, Preschool; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000096:biosynthesis; D020738:Leptin / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D009765:Obesity / Q000378:metabolism*; D052243:Resistin / Q000096:biosynthesis
chemical_list: D054392:Adipokines; D052242:Adiponectin; D015850:Interleukin-6; D020738:Leptin; D052243:Resistin; D009569:Nitric Oxide; D002097:C-Reactive Protein; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II