title: The sensitivity of two-dimensional hindlimb joint kinematics analysis in assessing functional recovery in rats after sciatic nerve crush.
abstract: Walking analysis in the rat is increasingly used to assess functional recovery after peripheral nerve injury. Here we assess the sensitivity and specificity of hindlimb joint kinematics measures during the rat gait early after sciatic nerve crush injury (DEN), after twelve weeks of recovery (REINN) and in sham-operated controls (Sham) using discriminant analysis. The analysis addressed gait spatiotemporal variables and hip, knee and ankle angle and angular velocity measures during the entire walking cycle. In DEN animals, changes affected all studied joints plus spatiotemporal parameters of gait. Both the spatiotemporal and ankle kinematics parameters recovered to normality within twelve weeks. At this time point, some hip and knee kinematics values were still abnormal when compared to sham controls. Discriminant models based on hip, knee and ankle kinematics displayed maximal sensitivity to identify DEN animals. However, the discriminant models based on spatiotemporal and ankle kinematics data showed a poor performance when assigning animals to the REINN and Sham groups. Models using hip and knee kinematics during walking showed the best sensitivity to recognize the reinnervated animals. The model construed on the basis of hip joint kinematics was the one combining highest sensitivity with robustness and high specificity. It is concluded that ankle joint kinematics fails in detecting minor functional deficits after long term recovery from sciatic nerve crush and extending the kinematic analysis during walking to the hip and knee joints improves the sensitivity of this functional test.
authors.affiliation: Faculty of Human Kinetics and Neuromechanics of Human Movement Group, CIPER, Technical University of Lisbon, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Amado
authors.forename: Paulo A S
authors.initials: PA
authors.lastname: Armada-da-Silva
authors.forename: Filipa
authors.initials: F
authors.lastname: João
authors.forename: Ana C
authors.initials: AC
authors.lastname: Maurício
authors.forename: Ana L
authors.initials: AL
authors.lastname: Luís
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Simões
authors.forename: António P
authors.initials: AP
authors.lastname: Veloso
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology*; D016002:Discriminant Analysis; D005684:Gait / Q000502:physiology*; D006614:Hindlimb / Q000502:physiology*; D007596:Joints / Q000502:physiology*; D008297:Male; D015233:Models, Statistical; D009409:Nerve Crush / Q000534:rehabilitation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D020426:Sciatic Neuropathy / Q000175:diagnosis / Q000503:physiopathology* / Q000534:rehabilitation; D012680:Sensitivity and Specificity; D013997:Time Factors