title: Novel cryo-imaging of the glioma tumor microenvironment reveals migration and dispersal pathways in vivid three-dimensional detail.
abstract: Traditional methods of imaging cell migration in the tumor microenvironment include serial sections of xenografts and standard histologic stains. Current molecular imaging techniques suffer from low resolution and difficulty in imaging through the skull. Here we show how computer algorithms can be used to reconstruct images from tissue sections obtained from mouse xenograft models of human glioma and can be rendered into three-dimensional images offering exquisite anatomic detail of tumor cell dispersal. Our findings identify human LN-229 and rodent CNS-1 glioma cells as valid systems to study the highly dispersive nature of glioma tumor cells along blood vessels and white matter tracts in vivo. This novel cryo-imaging technique provides a valuable tool to evaluate therapeutic interventions targeted at limiting tumor cell invasion and dispersal.
authors.affiliation: Department of Molecular Biology and Microbiology, Biomedical Engineering, Neuroscience, NFCR Center for Molecular Imaging, and Radiology, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Burden-Gulley
authors.forename: Mohammed Q
authors.initials: MQ
authors.lastname: Qutaish
authors.forename: Kristin E
authors.initials: KE
authors.lastname: Sullivant
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Sonya E L
authors.initials: SE
authors.lastname: Craig
authors.forename: James P
authors.initials: JP
authors.lastname: Basilion
authors.forename: David L
authors.initials: DL
authors.lastname: Wilson
authors.forename: Susann M
authors.initials: SM
authors.lastname: Brady-Kalnay
mesh_terms: D000465:Algorithms; D000818:Animals; D001932:Brain Neoplasms / Q000098:blood supply / Q000473:pathology*; D002465:Cell Movement; D015925:Cryopreservation; D005910:Glioma / Q000098:blood supply / Q000473:pathology*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional; D051379:Mice; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D059016:Tumor Microenvironment; D023041:Xenograft Model Antitumor Assays