title: Combined microwave irradiation and intraarticular glutamine administration-induced HSP70 expression therapy prevents cartilage degradation in a rat osteoarthritis model.
abstract: The objective of the present study was to investigate the effects of heat stimulation and glutamine (Gln) on the expression of extracellular matrix genes and heat shock protein 70 (HSP70) in rat articular cartilage in vivo and to determine whether HSP70 expression achieved with a combination of microwave (MW) and Gln suppresses osteoarthritis (OA) progression in a rat OA model. Stimulation at 40 W was assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. Articular cartilage was collected at 8 h after heat stimulation and/or intraarticular Gln administration, and total RNA was extracted. The expression of HSP70, aggrecan, and type II collagen was quantified using real-time RT-PCR. Cartilage samples from the OA model were subjected to hematoxylin and eosin (HE) and safranin O staining. HSP70 and aggrecan expression was greatest in a group receiving both MW and Gln. In the rat OA model, the severity of OA was significantly milder in a group receiving MW and Gln than in the control group. HSP70, stimulated by the combination of MW heat and Gln, may be involved in the suppression of OA progression.
authors.affiliation: Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Fujita
authors.forename: Yuji
authors.initials: Y
authors.lastname: Arai
authors.forename: Shuji
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Kenji A
authors.initials: KA
authors.lastname: Takahashi
authors.forename: Ryu
authors.initials: R
authors.lastname: Terauchi
authors.forename: Atsuo
authors.initials: A
authors.lastname: Inoue
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Tonomura
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hiraoka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Inoue
authors.forename: Shinji
authors.initials: S
authors.lastname: Tsuchida
authors.forename: Osam
authors.initials: O
authors.lastname: Mazda
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Kubo
mesh_terms: D053674:Aggrecans / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D002358:Cartilage, Articular / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D020868:Gene Silencing; D005973:Glutamine / Q000008:administration & dosage*; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D008872:Microwaves / Q000627:therapeutic use*; D010003:Osteoarthritis / Q000378:metabolism / Q000628:therapy*; D011794:Quercetin / Q000494:pharmacology / Q000627:therapeutic use; D034741:RNA, Small Interfering; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
chemical_list: D053674:Aggrecans; D000975:Antioxidants; D024043:Collagen Type II; D016326:Extracellular Matrix Proteins; D018840:HSP70 Heat-Shock Proteins; D034741:RNA, Small Interfering; D005973:Glutamine; D011794:Quercetin