title: Characterisation of oligosaccharides from the chondroitin/dermatan sulphates: (1)H and (13)C NMR studies of oligosaccharides generated by nitrous acid depolymerisation.
abstract: The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH(4) and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete (1)H and (13)C NMR data have been derived for the major components which were shown to have the structures: GlcA-(β1→3)-anTal6S-ol (I) and L-IdoA-(α1→3)-anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.
authors.affiliation: Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK. r.lauder@lancaster.ac.uk
authors.forename: Robert M
authors.initials: RM
authors.lastname: Lauder
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Huckerby
authors.forename: Ian A
authors.initials: IA
authors.lastname: Nieduszynski
authors.forename: Ian H
authors.initials: IH
authors.lastname: Sadler
mesh_terms: D002240:Carbohydrate Sequence; D002807:Chondroitin / Q000737:chemistry*; D003871:Dermatan Sulfate / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D007067:Iduronic Acid / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009608:Nitrous Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D058105:Polymerization
chemical_list: D009844:Oligosaccharides; D003871:Dermatan Sulfate; D007067:Iduronic Acid; D020723:Glucuronic Acid; D002807:Chondroitin; D009608:Nitrous Acid