title: Effect of platelet-rich plasma on growth and antigenic profile of human osteoblasts and its clinical impact.
abstract: OBJECTIVE
  In recent years, there has been widespread clinical use of platelet-rich plasma (PRP) to facilitate the regeneration of different tissues. However, few data are available on the effect of PRP on parameters other than cell growth. The aim of the present study was to evaluate the effect of PRP on the cell cycle, antigenic profile, and proliferation of primary cultured human osteoblasts.
  
  
  METHODS
  The cells in the present study were derived from human bone sections obtained from healthy volunteers during third molar surgery. PRP was prepared from human venous blood and used to culture the cell line obtained from the same patient. Flow cytometry was used to study the cell cycle, antigenic profile, and proliferation.
  
  
  RESULTS
  The treatment of osteoblasts with PRP modified the expression of CD54, CD80, CD86, and HLA-DR antigens. PRP treatment increased cell proliferation in the short term, but the cell proliferation capacity diminished in the long term, perhaps owing to cell exhaustion. No change in the cell cycle profile was observed in the PRP-cultured cells.
  
  
  CONCLUSIONS
  These results suggest that PRP treatment accelerates bone neoformation with no cell cycle changes that might carry a risk of malignant transformation.
authors.affiliation: Biomedical Research Group (BIO277), Department of Nursing, University of Granada School of Health Sciences, Granada, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: García-Martínez
authors.forename: Candela
authors.initials: C
authors.lastname: Reyes-Botella
authors.forename: Lourdes
authors.initials: L
authors.lastname: Díaz-Rodríguez
authors.forename: Elvira
authors.initials: E
authors.lastname: De Luna-Bertos
authors.forename: Javier
authors.initials: J
authors.lastname: Ramos-Torrecillas
authors.forename: Manuel Francisco
authors.initials: MF
authors.lastname: Vallecillo-Capilla
authors.forename: Concepción
authors.initials: C
authors.lastname: Ruiz
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D018826:CD13 Antigens / Q000032:analysis; D018929:Cell Culture Techniques; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007399:Interphase / Q000502:physiology; D015260:Neprilysin / Q000032:analysis; D010006:Osteoblasts / Q000276:immunology / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000502:physiology*; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497483:CD44 protein, human; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1; D000469:Alkaline Phosphatase; D018826:CD13 Antigens; D015260:Neprilysin