title: [Construction and identification of attenuated Salmonella which harboring enterovirus 71 VP1 gene].
abstract: OBJECTIVE
  To develop attenuated Salmonella which harboring enterovirus 71 (EV71) VP1 gene.
  
  
  METHODS
  The plasmid which expressed VP1 protein of EV71 was constructed by gene recombination. Cellular expression was assessed by Western Blot analysis. The recombinant plasmid was then transformed into attenuated Salmonella SL7207.
  
  
  RESULTS
  EV71 VP1 gene sequence was inserted into a eukaryotic expression plasmid VR1012. VP1 protein was detected by Western Blot analysis in the culture supernatant. And the attenuated Salmonella harbored the plasmid stable.
  
  
  CONCLUSIONS
  The plasmid was constructed successfully and it can express effectively in vitro. The bacteria which harboring the plasmid were constructed successfully. This has provided a basis for further research of an oral EV71 vaccine.
authors.affiliation: Military Postgraduate Medical College, Beijing 100853, China.
authors.forename: Ze
authors.initials: Z
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Hong-hui
authors.initials: HH
authors.lastname: Shen
authors.forename: Shao-ping
authors.initials: SP
authors.lastname: Cai
authors.forename: Bing-ke
authors.initials: BK
authors.lastname: Bai
authors.forename: Rong
authors.initials: R
authors.lastname: Gao
authors.forename: Sheng-dong
authors.initials: SD
authors.lastname: Luo
authors.forename: Yan-tao
authors.initials: YT
authors.lastname: Chai
authors.forename: Pan-yong
authors.initials: PY
authors.lastname: Mao
mesh_terms: D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D029821:Enterovirus A, Human / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D012475:Salmonella / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins