title: Microsatellite loci development in mycoheterotrophic Corallorhiza maculata (Orchidaceae) with amplification in C. mertensiana.
abstract: OBJECTIVE
  Microsatellite primers were developed for the first time in the species Corallorhiza maculata, a nonphotosynthetic orchid that is becoming a model for studying mycorrhizal specificity. •
  
  
  RESULTS
  Eight polymorphic microsatellite markers were developed using an enrichment and cloning protocol. The number of alleles for each locus ranged from two to seven. The loci were tested in three populations, and the resulting high F(ST) values suggested that these loci have utility in illuminating population structure. Cross-amplification was also achieved in the sister species C. mertensiana. •
  
  
  CONCLUSIONS
  These microsatellite markers will be useful in further study of the population genetics of this species, including that of previously described mycorrhizal races.
authors.affiliation: Department of Biology and Wildlife, University of Alaska Fairbanks, 211 Irving I building, Fairbanks, Alaska 99775 USA. sehopkins4@alaska.edu
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Hopkins
authors.forename: D Lee
authors.initials: DL
authors.lastname: Taylor
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005784:Gene Amplification; D017343:Genes, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D029595:Orchidaceae / Q000235:genetics*
chemical_list: D017931:DNA Primers