title: [Effects of inorganic arsenic on mRNA expression of Nrf2 and Nrf2-regulated downstream anti-oxidant enzymes in Chang hepatocytes].
abstract: OBJECTIVE
  To study the effects of inorganic arsenic on mRNA expression of transcription factor Nrf2 and Nrf2-regulated downstream anti-oxidant enzymes, NADPH: quinine oxidoreductase 1 (NQO1) and heme oxygenase 1(HO-1) in Chang hepatocytes.
  
  
  METHODS
  Chang hepatocytes were treated with 5, 10, 25 and 50 micromol/L of sodium arsenite (NaAsO2) for 6h, and RT-PCR were then performed to detect the mRNA expression of Nrf2, NQO1 and HO-1.
  
  
  RESULTS
  When exposed to 5, 10, 25 and 50 micromol/L of NaAsO2, Nrf2 mRNA were (100.74 +/- 3.70)%, (105.96 +/- 1.75)%, (101.76 +/- 1.01)% and (101.81 +/- 6.33)% of control,showing no statistic significance (P > 0.05). NQO1 mRNA expression were significantly increased (P < 0.05), and the levels of NQO1 mRNA were (106.52 +/- 3.11)%, (113.27 +/- 2.84)%, (111.96 +/- 6.96)% and (107.33 +/- 2.76)% of control, respectively. Moreover, HO-1 mRNA expression were also significantly induced by sodium arsenite exposure, and a definite dose-effect relationship was confirmed (P < 0.01).
  
  
  CONCLUSIONS
  No elevation of Nrf2 transcription was found, while inorganic arsenic could increase the Nrf2-regulated NQO1 and HO-1 mRNA expression in human hepatocytes.
authors.affiliation: Department of Occupational and Environmental Health, Liaoning Provincial Key Laboratory of Arsenic Biological Effect and Poisoning, School of Public Health, China Medical University, Shenyang 110001, China. libing@mail.cmu.edu.cn
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Xing
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Guifan
authors.initials: G
authors.lastname: Sun
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001151:Arsenic / Q000633:toxicity; D018053:Arsenites / Q000633:toxicity*; D002460:Cell Line; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D018053:Arsenites; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D012333:RNA, Messenger; D017670:Sodium Compounds; C017947:sodium arsenite; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D001151:Arsenic