title: The C2H2 zinc finger transcription factors are likely targets for Ni(II) toxicity.
abstract: Ni(II) ions are able to hydrolyze Naa-(Ser/Thr) peptide bonds in Naa-(Ser/Thr)-Xaa-His-Zaa sequences. We found that various human transcription factors contain such nickel hydrolytic patterns within C2H2 zinc finger (ZF) domains. We demonstrated the hydrolysis on two peptide models, the 3rd ZF of the Sp1 transcription factor and the 1st ZF of the ZNF302 transcription factor. The experimentally studied reaction rates indicate that the hydrolysis reaction is likely to be an element of intracellular nickel toxicity.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kurowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Sasin-Kurowska
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Bonna
authors.forename: Marcin
authors.initials: M
authors.lastname: Grynberg
authors.forename: Jarosław
authors.initials: J
authors.lastname: Poznański
authors.forename: Lukasz
authors.initials: L
authors.lastname: Knizewski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Ginalski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Bal
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009532:Nickel / Q000633:toxicity*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D010455:Peptides; D014157:Transcription Factors; D009532:Nickel