title: Anti-allergic effect of lambertianic acid from Thuja orientalis in mouse bone marrow-derived mast cells.
abstract: Lambertianic acid is a bioactive diterpene found in the leaves of Thuja orientalis. Its effect on the bone marrow-derived mast cell (BMMC) mediated allergy and inflammation mechanism remains unknown. In this study, lambertianic acid was evaluated for its effect on the allergic mediators, including prostaglandin D(2) (PGD(2)), leukotriene C(4) (LTC(4)), β-hexosaminidase (β-Hex) and cyclooxygenase-2 (COX-2) protein, in phorbol 12-myristate 13-acetate (PMA) plus calcimycin-stimulated BMMCs. The results revealed that lambertianic acid inhibited the production of interleukin-6 (IL-6), PGD(2) and LTC(4), the expression of COX-2 and the degranulation of β-hexosaminidase in the PMA plus calcimycin-induced BMMCs. Taken together, these findings implied that lambertianic acid may possess the potential in the treatment of allergy.
authors.affiliation: Immune Modulator Research Center, Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience & Biotechnology, Korea.
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Chae
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology*; D000001:Calcimycin / Q000494:pharmacology; D002264:Carboxylic Acids / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D018628:Gene Dosage / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D017997:Leukotriene C4 / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009281:Naphthalenes / Q000494:pharmacology*; D015230:Prostaglandin D2 / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D029783:Thuja / Q000737:chemistry*; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D002264:Carboxylic Acids; D015850:Interleukin-6; D009281:Naphthalenes; D017997:Leukotriene C4; D000001:Calcimycin; C421442:lambertianic acid; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D001619:beta-N-Acetylhexosaminidases; D013755:Tetradecanoylphorbol Acetate; D015230:Prostaglandin D2