title: Molecular machines in archaeal DNA replication.
abstract: The archaeal DNA replication apparatus is a simplified version of that of eukaryotes and has attracted attention as a tractable model system for the orthologous, but significantly more complex eukaryal machinery. A variety of archaeal model organisms have been investigated with strong emphasis on structural and biochemical analyses of replication-associated proteins. In this review we will describe recent advances in understanding the properties of the replicative helicase, the MCM complex, and the role of the sliding clamp, PCNA, in mediating a range of protein-DNA transactions. Although both complexes form ring shaped assemblies, they play very distinct roles at the leading and trailing edges of the replication fork machinery respectively.
authors.affiliation: Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK.
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Beattie
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Bell
mesh_terms: D001105:Archaea / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001696:Biomechanical Phenomena; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004265:DNA Helicases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000235:genetics*; D019641:DNA, Archaeal / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs; D016914:Ribonuclease H / Q000235:genetics / Q000378:metabolism*
chemical_list: D019843:Archaeal Proteins; D019641:DNA, Archaeal; D004268:DNA-Binding Proteins; C022262:Okazaki fragments; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA; C091139:ribonuclease HII; D016914:Ribonuclease H; D004265:DNA Helicases