title: Regulatory T cells limit vascular endothelial injury and prevent pulmonary hypertension.
abstract: BACKGROUND
  Pulmonary arterial hypertension (PAH) is an incurable disease associated with viral infections and connective tissue diseases. The relationship between inflammation and disease pathogenesis in these disorders remains poorly understood.
  
  
  OBJECTIVE
  To determine whether immune dysregulation due to absent T-cell populations directly contributes to the development of PAH.
  
  
  RESULTS
  Vascular endothelial growth factor receptor 2 (VEGFR2) blockade induced significant pulmonary endothelial apoptosis in T-cell-deficient rats but not in immune-reconstituted (IR) rats. T cell-lymphopenia in association with VEGFR2 blockade resulted in periarteriolar inflammation with macrophages, and B cells even prior to vascular remodeling and elevated pulmonary pressures. IR prevented early inflammation and attenuated PAH development. IR with either CD8 T cells alone or with CD4-depleted spleen cells was ineffective in preventing PAH, whereas CD4-depleting immunocompetent euthymic animals increased PAH susceptibility. IR with either CD4(+)CD25(hi) or CD4(+)CD25(-) T cell subsets prior to vascular injury attenuated the development of PAH. IR limited perivascular inflammation and endothelial apoptosis in rat lungs in association with increased FoxP3(+), IL-10- and TGF-β-expressing CD4 cells, and upregulation of pulmonary bone morphogenetic protein receptor type 2 (BMPR2)-expressing cells, a receptor that activates endothelial cell survival pathways.
  
  
  CONCLUSIONS
  PAH may arise when regulatory T-cell (Treg) activity fails to control endothelial injury. These studies suggest that regulatory T cells normally function to limit vascular injury and may protect against the development of PAH.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Stanford University School of Medicine, VA Palo Health Care System, CA 94304, USA.
authors.forename: Rasa
authors.initials: R
authors.lastname: Tamosiuniene
authors.forename: Wen
authors.initials: W
authors.lastname: Tian
authors.forename: Gundeep
authors.initials: G
authors.lastname: Dhillon
authors.forename: Lijuan
authors.initials: L
authors.lastname: Wang
authors.forename: Yon K
authors.initials: YK
authors.lastname: Sung
authors.forename: Lajos
authors.initials: L
authors.lastname: Gera
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Patterson
authors.forename: Rani
authors.initials: R
authors.lastname: Agrawal
authors.forename: Marlene
authors.initials: M
authors.lastname: Rabinovitch
authors.forename: Kelly
authors.initials: K
authors.lastname: Ambler
authors.forename: Carlin S
authors.initials: CS
authors.lastname: Long
authors.forename: Norbert F
authors.initials: NF
authors.lastname: Voelkel
authors.forename: Mark R
authors.initials: MR
authors.lastname: Nicolls
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000276:immunology* / Q000473:pathology; D006976:Hypertension, Pulmonary / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011923:Rats, Nude; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D057772:Vascular System Injuries / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*