title: [Influence of surface modification of titanium on OPG/RANKL mRNA expression in MG-63 human osteoblast-like cells].
abstract: OBJECTIVE
  To investigate the influence of surface modification of titanium on OPG/RANKL mRNA expression in human osteoblast-like cells.
  
  
  METHODS
  MG-63 osteoblast-like cells were seeded on the titanium plates with surface polishing and with surface modification by sandblasting plus acid-base treatment, with the cells on glass slides as the control. On days 2, 4, 6, and 8 following cell seeding, the cells were harvested for examination of OPG/RANKL mRNA expression using RT-PCR and real-time PCR.
  
  
  RESULTS
  The expression of OPG/RANKL mRNA was sensitive to the surface microphotography. Compared with the other groups, the cells on the titanium plates with sandblasting plus acid-base treatment, which resulted in a porous micro-structure and high roughness, showed significantly up-regulated expression of OPG mRNA. OPG mRNA expression also showed a time-dependent up-regulation, and was the highest on day 8. The expression of the RANKL mRNA in cells on both of the titanium plates was higher than that in the control cells. The peak level of RANKL mRNA expression occurred on day 6 followed by a gradual decrease.
  
  
  CONCLUSIONS
  A rough and porous surface of the culture plates and prolonged culture time can synergistically up-regulate the ratio of OPG/RANKL mRNA.
authors.affiliation: Department of Dental Implantation, Southern Medical University, Guangzhou, China. liuyang197374@sina.com
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Yang
authors.forename: Chang-hong
authors.initials: CH
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Lei
authors.forename: Yuan
authors.initials: Y
authors.lastname: Su
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Li
authors.forename: Hua-ying
authors.initials: HY
authors.lastname: Wang
authors.forename: Wei-cheng
authors.initials: WC
authors.lastname: Xu
authors.forename: Su-qin
authors.initials: SQ
authors.lastname: Xian
mesh_terms: D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*; D016062:Porosity; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013499:Surface Properties; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; C506030:TNFRSF11B protein, human; C505688:TNFSF11 protein, human; D014025:Titanium