title: Detection of aneuploidy rate for chromosomes X, Y and 8 by fluorescence in-situ hybridization in spermatozoa from patients with severe non-obstructive oligozoospermia.
abstract: OBJECTIVE
  To evaluate the frequency of sperm nuclei disomy for chromosomes 8, X, and Y in patients with severe non-obstructive oligozoospermia and to assess possible correlations between sperm nuclei aneuploidy and semen parameters or a particular clinical phenotype.
  
  
  METHODS
  The sperm aneuploidy rate for chromosomes X, Y, and 8 were assessed in 16 infertile men with severe non-obstructive oligozoospermia and 7 healthy men with normal semen parameters. The frequency of sperm aneuploidy was compared between several patients groups according to their clinical and biological factors.
  
  
  RESULTS
  The total rate of chromosomally abnormal spermatozoa was significantly higher in patients with severe oligozoospermia compared to control group (P < 0.001). A significant relationship was found between the age of patients, sperm concentration, and morphology and the mean rate of sex chromosomes disomy. In addition to the low sperm count (<5 × 10(6)/ml), an elevated FSH level and an exposed to an elevated temperature are two major predictive factors leading to the production of higher numbers of chromosomally abnormal gametes.
  
  
  CONCLUSIONS
  Patients with severe oligozoospermia, who are potential candidates for assisted reproduction technology, presented a high level of sex numerical chromosome abnormalities, and consequently are at high risk of chromosome abnormalities in their offspring.
authors.affiliation: Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, Sousse, 4000, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Mougou-Zerelli
authors.forename: Sonia
authors.initials: S
authors.lastname: Brahem
authors.forename: Molka
authors.initials: M
authors.lastname: Kammoun
authors.forename: Mehdi
authors.initials: M
authors.lastname: Jerbi
authors.forename: Hatem
authors.initials: H
authors.lastname: Elghezal
authors.forename: Mounir
authors.initials: M
authors.lastname: Ajina
authors.forename: Ali
authors.initials: A
authors.lastname: Saad
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002898:Chromosomes, Human, Pair 8; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D055101:Semen Analysis; D013094:Spermatozoa