title: Membrane-bound alcohol dehydrogenase is essential for glyceric acid production in Acetobacter tropicalis.
abstract: Acetobacter tropicalis NBRC16470 can produce highly enantiomerically pure D-glyceric acid (D-GA; >99 % enantiomeric excess) from glycerol. To investigate whether membrane-bound alcohol dehydrogenase (mADH) is involved in GA production in A. tropicalis, we amplified part of the gene encoding mADH subunit I (adhA) using polymerase chain reaction and constructed an adhA-disrupted mutant of A. tropicalis (ΔadhA). Because ΔadhA did not produce GA, we confirmed that mADH is essential for the conversion of glycerol to GA. We also cloned and sequenced the entire region corresponding to adhA and adhB, which encodes mADH subunit II. The sequences showed high identities (84-86 %) with the equivalent mADH subunits from other Acetobacter spp.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki. hiroshi.habe@aist.go.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.forename: Shun
authors.initials: S
authors.lastname: Sato
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Yakushi
authors.forename: Kazunobu
authors.initials: K
authors.lastname: Matsushita
authors.forename: Keiji
authors.initials: K
authors.lastname: Sakaki
mesh_terms: D000091:Acetobacter / Q000201:enzymology* / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005988:Glyceric Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D005988:Glyceric Acids; C042971:glyceric acid; D000426:Alcohol Dehydrogenase