title: Validation of the Seratec® SeraQuant™ for the quantitation of prostate-specific antigen levels on immunochromatographic membranes.
abstract: Use of immunochromatographic membranes for the detection of prostate-specific antigen (PSA) has become commonplace in forensic laboratories. Experiments were designed to test the newly developed Seratec(®)  SeraQuant™ for accuracy, precision, and consistency in the quantitation of PSA. PSA standards were diluted with buffers and run on the instruments. Values obtained were examined for accuracy (was the correct value obtained?) and precision (were multiple sample values consistent?). To test for variation between instruments, large volumes of diluted PSA standard were run repeatedly on six units and the values obtained were plotted against the known PSA values to obtain a standard curve for each instrument. Fifty membranes having negative or weak positive results were then run on the six units, and the adjusted values were recorded and compared. Results of these experiments indicate that the instruments are accurate and precise in the quantitation of low levels of PSA.
authors.affiliation: Ohio Attorney General's Office, Bureau of Criminal Identification and Investigation, Richfield, OH 44286, USA. d.l.laux@csuohio.edu
authors.forename: Dale L
authors.initials: DL
authors.lastname: Laux
authors.forename: Justin P
authors.initials: JP
authors.lastname: Barnhart
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis*; D012984:Software
chemical_list: D017430:Prostate-Specific Antigen