title: Fucosyltransferases as synthetic tools: glycan array based substrate selection and core fucosylation of synthetic N-glycans.
abstract: Two recombinant fucosyltransferases were employed as synthetic tools in the chemoenzymatic synthesis of core fucosylated N-glycan structures. Enzyme substrates were rapidly identified by incubating a microarray of synthetic N-glycans with the transferases and detecting the presence of core fucose with four lectins and one antibody. Selected substrates were then enzymatically fucosylated in solution on a preparative scale and characterized by NMR and MS. With this approach the chemoenzymatic synthesis of a series of α1,3-, α1,6-, and difucosylated structures was accomplished in very short time and with high yields, which otherwise would have required extensive additional synthetic effort and a complete redesign of existing synthetic routes. In addition, valuable information was gathered regarding the specificities of the lectins employed in this study.
authors.affiliation: Biofunctional Nanomaterials Unit, CICbiomaGUNE, Paseo Miramon 182, 20009 San Sebastian, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Serna
authors.forename: Shi
authors.initials: S
authors.lastname: Yan
authors.forename: Manuel
authors.initials: M
authors.lastname: Martin-Lomas
authors.forename: Iain B H
authors.initials: IB
authors.lastname: Wilson
authors.forename: Niels-Christian
authors.initials: NC
authors.lastname: Reichardt
mesh_terms: D002236:Carbohydrate Conformation; D005647:Fucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D011134:Polysaccharides; D005647:Fucosyltransferases