title: [Procedure of running experiments on studying the effects of altered gas medium on culture of hydra Hydra attenuata].
abstract: Owing to the simple form and short gemmation, hydroid polyps have become a classic model object for studies into cell division and proliferation, and effects of changed environment on these processes. However, there is no procedure for studying of how altered gas composition of aqueous medium affects biological processes in hydroidpolyps. Testing of a procedure for objective studies with bioculture of Hydra attenuata resulted in determination of optimal hydra density in Petri dish. It was also stated that culture duplication takes minimal time at 25-30 degrees C. Tests verified efficiency of an incubator designed for experiment with Hydra attenuata in pressurized chambers enabling controlled ventilation by different gas mixtures. It was shown that time of culture duplication in normoxic and hypoxic nitrox is permanent and statistically different Therefore, the proposed procedure and incubator can be useful for fundamental and applied studies of the biological effects of altered gas medium on hydroid polyps.
authors.forename: I R
authors.initials: IR
authors.lastname: Ruzhichko
authors.forename: B N
authors.initials: BN
authors.lastname: Pavlov
mesh_terms: D000818:Animals; D002452:Cell Count; D002455:Cell Division; D003470:Culture Media / Q000737:chemistry*; D005740:Gases / Q000032:analysis*; D006829:Hydra / Q000166:cytology / Q000254:growth & development*; D013997:Time Factors
chemical_list: D003470:Culture Media; D005740:Gases