title: A broader role for AmyR in Aspergillus niger: regulation of the utilisation of D-glucose or D-galactose containing oligo- and polysaccharides.
abstract: AmyR is commonly considered a regulator of starch degradation whose activity is induced by the presence of maltose, the disaccharide building block of starch. In this study, we demonstrate that the role of AmyR extends beyond starch degradation. Enzyme activity assays, genes expression analysis and growth profiling on D-glucose- and D-galactose-containing oligo- and polysaccharides showed that AmyR regulates the expression of some of the Aspergillus niger genes encoding α- and β-glucosidases, α- and β- galactosidases, as well as genes encoding α-amlyases and glucoamylases. In addition, we provide evidence that D-glucose or a metabolic product thereof may be the inducer of the AmyR system in A. niger and not maltose, as is commonly assumed.
authors.affiliation: Molecular Genetics of Industrial Microorganisms, Wageningen University, Wageningen, The Netherlands.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: vanKuyk
authors.forename: Jaques A E
authors.initials: JA
authors.lastname: Benen
authors.forename: Han A B
authors.initials: HA
authors.lastname: Wösten
authors.forename: Jaap
authors.initials: J
authors.lastname: Visser
authors.forename: Ronald P
authors.initials: RP
authors.lastname: de Vries
mesh_terms: D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism*; D009548:Niger; D011134:Polysaccharides / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D015534:Trans-Activators; C414005:amyR protein, Aspergillus; D005947:Glucose; D005690:Galactose