title: Yan, an ETS-domain transcription factor, negatively modulates the Wingless pathway in the Drosophila eye.
abstract: We report the identification of yan, an ETS-domain transcription factor belonging to the Drosophila epidermal growth factor receptor (DER) pathway, as an antagonist of the Wingless signalling pathway. We demonstrate that cells lacking yan function in the Drosophila eye show increased Wingless pathway activity, and inhibition of Wingless signalling in yan(-/-) cells rescues the yan mutant phenotype. Biochemical analysis shows that Yan physically associates with Armadillo, a crucial effector of the Wingless pathway, thereby suggesting a direct regulatory mechanism. We conclude that yan represents a new and unsuspected molecular link between the Wingless and DER pathways.
authors.affiliation: Cancer Institute and Department of Pharmacology, New York University School of Medicine, 522 First Avenue, Smilow Research Building, Room 1211, New York, New York 10016, USA.
authors.forename: Emily R
authors.initials: ER
authors.lastname: Olson
authors.forename: Raluca
authors.initials: R
authors.lastname: Pancratov
authors.forename: Sujash S
authors.initials: SS
authors.lastname: Chatterjee
authors.forename: Binita
authors.initials: B
authors.lastname: Changkakoty
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Pervaiz
authors.forename: Ramanuj
authors.initials: R
authors.lastname: DasGupta
mesh_terms: D000818:Animals; D051186:Armadillo Domain Proteins / Q000037:antagonists & inhibitors; D002460:Cell Line; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D011485:Protein Binding; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000037:antagonists & inhibitors; D051155:Wnt1 Protein / Q000378:metabolism*
chemical_list: C076188:AOP protein, Drosophila; C495305:ARM protein, Drosophila; D051186:Armadillo Domain Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C074622:wg protein, Drosophila