title: Recombinant Shiga toxin B subunit elicits protection against Shiga toxin via mixed Th type immune response in mice.
abstract: Shigella dysenteriae is the causative agent of the third commonest bacterial disease for childhood diarrhoea and responsible for millions of deaths per year. It produces potent toxin termed Shiga toxin which is listed in category B biological warfare agent of CDC, USA. Earlier we have reported production of recombinant Shiga toxin B subunit that produced antibodies which neutralized Shiga toxin toxicity in HeLa cells. In the present study, we have evaluated the immunomodulatory potential of rStxB protein in Balb/c mice using Freunds adjuvants. Animal protection with recombinant StxB was conferred through both humoral and cellular immune responses as indicated by an increased antibody titre with predominance of IgG2a and IgG2b isotypes along with elevated levels of IgG1 subtype. Cytokine profile of the mice antiserum and splenocyte also indicates Th2 and Th1 type of immune responses where former dominates in early stage of immunization. Histopathology study of kidneys of vaccinated mice showed remarkable differences when compared to the animals infected with Shigella dysenteriae type1. The present study indicates that recombinant StxB is a promising vaccine candidate and can be used for production of therapeutic antibodies to counter Shiga intoxication.
authors.affiliation: Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, MP, India.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Gupta
authors.forename: Manglesh Kumar
authors.initials: MK
authors.lastname: Singh
authors.forename: Yamini
authors.initials: Y
authors.lastname: Singh
authors.forename: Vandana
authors.initials: V
authors.lastname: Gautam
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
authors.forename: Om
authors.initials: O
authors.lastname: Kumar
authors.forename: Ram Kumar
authors.initials: RK
authors.lastname: Dhaked
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000992:Antitoxins / Q000097:blood; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004405:Dysentery, Bacillary / Q000473:pathology / Q000517:prevention & control; D005260:Female; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000097:blood; D007668:Kidney / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D021122:Protein Subunits / Q000008:administration & dosage / Q000276:immunology; D022621:Shiga Toxin / Q000008:administration & dosage / Q000276:immunology*; D022281:Shigella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012762:Shigella dysenteriae / Q000276:immunology / Q000472:pathogenicity; D013154:Spleen / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000992:Antitoxins; D016207:Cytokines; D007074:Immunoglobulin G; D021122:Protein Subunits; D022281:Shigella Vaccines; D014614:Vaccines, Synthetic; D022621:Shiga Toxin; D005620:Freund's Adjuvant