title: Effect of dietary supplementation with unprocessed and ethanol-extracted apple pomaces on caecal fermentation, antioxidant and blood biomarkers in rats.
abstract: The present 4-week study on growing Wistar rats was aimed at assessing the potential advantages of dietary supplementation with apple pomace containing both fibre and polyphenols, which enables nutritional exploitation of the physiological traits of both compounds. A total of twenty-four rats, assigned to the control (C), group fed with a diet supplemented with 14% of processed apple pomace (A) and group fed with a diet supplemented with 15% unprocessed apple pomace (AP) groups, were fed the following iso-fibrous diets: control, ethanol-extracted and unprocessed apple pomaces (low and high level of dietary polyphenols, i.e. 0·002 and 0·018%, respectively). To measure the animal response, parameters describing the caecal fermentation, antioxidative status and lipoprotein profile of rats were assessed. Both dietary apple pomaces were found to significantly (P≤0·05) decrease caecal pH and ammonia concentration, microbial β-glucuronidase activity as well as to increase caecal SCFA concentration in comparison to the control diet. The unprocessed pomace did not suppress caecal fermentation. Unlike the extracted one, the dietary apple pomace rich in polyphenols significantly (P≤0·05 v. C group) increased erythrocyte superoxide dismutase activity and serum antioxidant capacity of lipid-soluble substances and significantly (P≤0·05 v. C group) decreased amounts of thiobarbituric acid-reactive substances in liver tissue. Moreover, the 4-week administration of the AP diet to rats evoked a significant decrease in serum glucose concentration (P≤0·05 v. C and A groups). In conclusion, the results demonstrated that the polyphenol-rich fibre complexes from apple pomace exerted positive effects on gastrointestinal physiology and antioxidant status of rats.
authors.affiliation: Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10 Tuwima Str., 10-747 Olsztyn, Poland. j.juskiewicz@pan.olsztyn.pl
authors.forename: Jerzy
authors.initials: J
authors.lastname: Juśkiewicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Zary-Sikorska
authors.forename: Zenon
authors.initials: Z
authors.lastname: Zduńczyk
authors.forename: Bogusław
authors.initials: B
authors.lastname: Król
authors.forename: Julia
authors.initials: J
authors.lastname: Jarosławska
authors.forename: Adam
authors.initials: A
authors.lastname: Jurgoński
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002432:Cecum / Q000378:metabolism / Q000382:microbiology; D004043:Dietary Fiber / Q000008:administration & dosage; D019587:Dietary Supplements; D000431:Ethanol; D005285:Fermentation; D005511:Food Handling; D005966:Glucuronidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000097:blood; D008297:Male; D027845:Malus / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D059808:Polyphenols / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D004043:Dietary Fiber; D008055:Lipids; D010936:Plant Extracts; D059808:Polyphenols; D000431:Ethanol; D005966:Glucuronidase