title: Plasma pharmacochemistry based approach to screening potential bioactive components in Huang-Lian-Jie-Du-Tang using high performance liquid chromatography coupled with mass spectrometric detection.
abstract: BACKGROUND
  Huang-Lian-Jie-Du-Tang (HLJDT), a classic prescription of traditional Chinese medicine (TCM), has been used in clinical over 1700 years for the treatment of gastrointestinal disorders, cardiovascular diseases and Alzheimer disease. But the active components of HLJDT were ambiguous, which seriously restricted its clinical application.
  
  
  METHODS
  The methodology of plasma pharmacochemistry was applied to screen the bioactive components in HLJDT. A reliable LC/MS system was established for detecting the prototype compounds and metabolites in dosed plasma after oral administration of HLJDT. By comparative analysis of the chemical profiles of HLJDT extracts, blank plasma and dosed plasma, potential bioactive compounds in HLJDT may be discovered.
  
  
  RESULTS
  By comparing the retention time, MS and MS/MS spectra with those of reference standard and literature data, 30 components including 22 prototype compounds and 8 metabolites from HLJDT were discovered as potential bioactive components in rat plasma.
  
  
  CONCLUSIONS
  A reliable and effective method was established to screen the potential bioactive components in the formula of HLJDT, which provided useful information for the further study of action mechanism of HLJDT.
authors.affiliation: School of Pharmacy, Second Military Medical University, Shanghai, PR China.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Hu
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.forename: Shikai
authors.initials: S
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.forename: Runhui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001711:Biotransformation; D001769:Blood / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D004365:Drugs, Chinese Herbal; C045607:huang-lien-chieh-tu-tang