title: [Detection and genotyping of rotavirus in diarrhea infant feces in Chengdu].
abstract: OBJECTIVE
  To study the genotype of rotavirus and the genetic variations of the major neutralization antigen VP4 of group A rotavirus in fecal samples from infants with diarrhea in Chengdu, Sichuan province, China.
  
  
  METHODS
  The fecal specimens were collected from infant patients with diarrhea in the spring of 2010 at West China Second University Hospital. Reverse transcription polymerase chain reaction (RT-PCR) was performed to identify rotavirus G serotypes and P genotypes. VP4 gene fragments of the virus were amplified from two strains drawn randomly from the prevailing genotype and cloned into a T-A clone vector to generate the recombinants for sequencing.
  
  
  RESULTS
  A group rotaviruses were detected in 13 of 75 specimens (17.3%). Serotype G1 was the predominant type (7/13) and two were serotype G3, four strains' serotypes were unidentified. Analysis of P gene demonstrated that genotype P [8] was the predominant type (6/13), whereas only two P[4] genotype were detected and genotypes for two strains were not determined. G1P [8] was the predominant type of G/P dominance combination (5/13). Sequencing results of the VP4 gene for the analyzed two strains implied that they were genotype P[8] with a 97% homology in sequence. Compared with the standard strain, homologies were also more than 90%.
  
  
  CONCLUSIONS
  Rotavirus is one of the major etiological agents of viral diarrhea among infants in Chengdu. G1 was the dominant type G in Chengdu. G1P[8] was the predominant type of G/P dominance combination.
authors.affiliation: Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Liao
authors.forename: Meng-Yan
authors.initials: MY
authors.lastname: Zhang
authors.forename: Xi-Kai
authors.initials: XK
authors.lastname: Chen
authors.forename: Zhi-Jin
authors.initials: ZJ
authors.lastname: Chen
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Fan
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Pei
mesh_terms: D036022:Capsid Proteins / Q000235:genetics*; D002681:China; D003968:Diarrhea, Infantile / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus