title: In vitro and in vivo pro-angiogenic effects of thymosin-β4-derived peptides.
abstract: Thymosin-β4 (Tβ4) is a G-actin sequestering peptide involved in regeneration and remodeling of injured tissues. In this work, we have designed and synthesized three peptide sequences containing the N-terminus (TYB4-n), the central part (TYB4-i) or the C-terminus (TYB4-c) of Tβ4. All fragments are overlapping on the main central binding actin site. After a structural characterization, we have evaluated in vitro and in vivo their pro-angiogenic effects. The results of this study have shown that: (i) each fragment reproduces the native conformation; (ii) Tβ4-derived peptides exert both in vitro and in vivo pro-angiogenic effects; (iii) their in vitro effect seem to be related to the activation of several signaling pathways and is positively modulated by the N-terminus of Tβ4.
authors.affiliation: Department of Chemical Process Engineering, University of Padua, Padua, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
authors.forename: Francesca
authors.initials: F
authors.lastname: Ghezzo
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Conconi
authors.forename: Luca
authors.initials: L
authors.lastname: Urbani
authors.forename: Gabriella
authors.initials: G
authors.lastname: D'Auria
authors.forename: Lucia
authors.initials: L
authors.lastname: Falcigno
authors.forename: Diego
authors.initials: D
authors.lastname: Guidolin
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
authors.forename: Carlo
authors.initials: C
authors.lastname: Di Bello
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Parnigotto
mesh_terms: D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002642:Chick Embryo; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D011487:Protein Conformation; D015398:Signal Transduction / Q000187:drug effects; D013947:Thymosin / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology; D000099201:Thymosin beta(4)
chemical_list: D043925:Angiogenesis Inducing Agents; D010446:Peptide Fragments; D013947:Thymosin; D000099201:Thymosin beta(4)