title: Trolox reduces the effect of ethanol on acetylcholine-induced contractions and oxidative stress in the isolated rabbit duodenum.
abstract: Trolox is a hydrophilic analogue of vitamin E and a free radical scavenger. Ethanol diminishes the amplitude of spontaneous contractions and acetylcholine (ACh)-induced contractions in rabbit duodenum. The aim of this work was to study the effect of Trolox on the alterations induced by ethanol on contractility and lipid peroxidation in the duodenum. The duodenal contractility studies in vitro were carried out in an organ bath and the levels of malondialdehyde and 4-hydroxyalkenals (MDA+4-HAD) were measured by spectrophotometry. Trolox increased the reduction induced by ethanol on the amplitude of spontaneous contractions in longitudinal muscle but not in circular muscle. Trolox 4 mM decreased the effects of ethanol on ACh-induced contractions and on MDA+4-HDA concentrations. We conclude that Trolox might prevent oxidative stress induced by ethanol in the duodenum.
authors.affiliation: Department of Pharmacology and Physiology, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
authors.forename: Diego S
authors.initials: DS
authors.lastname: Fagundes
authors.forename: Sergio
authors.initials: S
authors.lastname: Gonzalo
authors.forename: Laura
authors.initials: L
authors.lastname: Grasa
authors.forename: Marta
authors.initials: M
authors.lastname: Castro
authors.forename: Ma Pilar
authors.initials: MP
authors.lastname: Arruebo
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Plaza
authors.forename: Ma Divina
authors.initials: MD
authors.lastname: Murillo
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology*; D004386:Duodenum / Q000187:drug effects* / Q000502:physiology*; D000431:Ethanol / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D009119:Muscle Contraction / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011817:Rabbits
chemical_list: D000975:Antioxidants; D002839:Chromans; D000431:Ethanol; D000109:Acetylcholine; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid