title: Human pathogen shown to cause disease in the threatened eklhorn coral Acropora palmata.
abstract: Coral reefs are in severe decline. Infections by the human pathogen Serratia marcescens have contributed to precipitous losses in the common Caribbean elkhorn coral, Acropora palmata, culminating in its listing under the United States Endangered Species Act. During a 2003 outbreak of this coral disease, called acroporid serratiosis (APS), a unique strain of the pathogen, Serratia marcescens strain PDR60, was identified from diseased A. palmata, human wastewater, the non-host coral Siderastrea siderea and the corallivorous snail Coralliophila abbreviata. In order to examine humans as a source and other marine invertebrates as vectors and/or reservoirs of the APS pathogen, challenge experiments were conducted with A. palmata maintained in closed aquaria to determine infectivity of strain PDR60 from reef and wastewater sources. Strain PDR60 from wastewater and diseased A. palmata caused disease signs in elkhorn coral in as little as four and five days, respectively, demonstrating that wastewater is a definitive source of APS and identifying human strain PDR60 as a coral pathogen through fulfillment of Koch's postulates. A. palmata inoculated with strain PDR60 from C. abbreviata showed limited virulence, with one of three inoculated fragments developing APS signs within 13 days. Strain PDR60 from non-host coral S. siderea showed a delayed pathogenic effect, with disease signs developing within an average of 20 days. These results suggest that C. abbreviata and non-host corals may function as reservoirs or vectors of the APS pathogen. Our results provide the first example of a marine "reverse zoonosis" involving the transmission of a human pathogen (S. marcescens) to a marine invertebrate (A. palmata). These findings underscore the interaction between public health practices and environmental health indices such as coral reef survival.
authors.affiliation: Department of Biology, Rollins College, Winter Park, Florida, United States of America. kpsutherland@rollins.edu
authors.forename: Kathryn Patterson
authors.initials: KP
authors.lastname: Sutherland
authors.forename: Sameera
authors.initials: S
authors.lastname: Shaban
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Joyner
authors.forename: James W
authors.initials: JW
authors.lastname: Porter
authors.forename: Erin K
authors.initials: EK
authors.lastname: Lipp
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D017691:Caribbean Region; D057894:Coral Reefs; D056727:Endangered Species; D005431:Florida; D054884:Host-Pathogen Interactions; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D016868:Serratia Infections / Q000382:microbiology*; D012706:Serratia marcescens / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D012722:Sewage / Q000382:microbiology; D012908:Snails / Q000382:microbiology; D013997:Time Factors
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage