title: A novel mitotropic oligolysine nanocarrier: Targeted delivery of covalently bound D-Luciferin to cell mitochondria.
abstract: New and emerging therapeutic approaches focus on the targeted delivery of therapeutic agents to cell mitochondria with high specificity. Herein we present a novel mitotropic nanocarrier based on an oligolysine scaffold by addition of two triphenylphosphonium cations per oligomer. Although the parent oligolysine failed to enter healthy cells, the triphenylphosphonium modified carrier, with or without D-Luciferin, attached as cargo molecule, demonstrated striking mitochondrial specificity. Furthermore, the oligolysine bound d-Luciferin exhibited chemiluminescence, of lower intensity than free d-Luciferin, yet of remarkably longer steady-state temporal profile.
authors.affiliation: Institute of Physical Chemistry, NCSR Demokritos, Patriarchou Gregoriou & Neapoleos, 153 10 Aghia Paraskevi, Attiki, Greece. theo@chem.demokritos.gr
authors.forename: Theodossis A
authors.initials: TA
authors.lastname: Theodossiou
authors.forename: Zili
authors.initials: Z
authors.lastname: Sideratou
authors.forename: Dimitris
authors.initials: D
authors.lastname: Tsiourvas
authors.forename: Constantinos M
authors.initials: CM
authors.lastname: Paleos
mesh_terms: D052160:Benzothiazoles / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism / Q000493:pharmacokinetics; D021381:Protein Transport
chemical_list: D052160:Benzothiazoles; D002352:Carrier Proteins; C532924:D-luciferin; D024101:Mitochondrial Proteins; D016566:Organoselenium Compounds; C092819:triphenylselenonium chloride; D011107:Polylysine