title: Detection of Aspergillus in lung and other tissue samples using the MycAssay Aspergillus real-time PCR kit.
abstract: The MycAssay™ Aspergillus real-time PCR kit was tested on tissues from patients with invasive fungal infections. Tissue samples from nine organ transplant recipients and 33 patients with haematological malignancy were from lung (n = 30), skin (n = 4), and others. Samples were preprocessed with proteinase K and lyticase, followed by DNA extraction and real-time PCR. For all samples, the sensitivity of the MycAssay Aspergillus test was 82% and specificity 79% relative to microscopy and 90% and 64%, respectively, compared with Aspergillus culture. The positive predictive value and negative predictive values compared with culture were 69% and 88% and were 88% and 69% compared with microscopy, respectively. The MycAssay Aspergillus test detected tissue invasive infections with Aspergillus fumigatus , Aspergillus flavus , and Aspergillus terreus.
authors.affiliation: Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Fritz-Pregl-Straße 3, 6020 Innsbruck, Austria. cornelia.lass-floerl@i-med.ac.at
authors.forename: C
authors.initials: C
authors.lastname: Lass-Flörl
authors.forename: S A
authors.initials: SA
authors.lastname: Follett
authors.forename: A
authors.initials: A
authors.lastname: Moody
authors.forename: D W
authors.initials: DW
authors.lastname: Denning
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis* / Q000382:microbiology; D001230:Aspergillus / Q000235:genetics* / Q000302:isolation & purification; D001231:Aspergillus flavus / Q000235:genetics / Q000302:isolation & purification; D001232:Aspergillus fumigatus / Q000235:genetics / Q000302:isolation & purification; D001681:Biological Assay / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D006801:Humans; D008168:Lung / Q000382:microbiology*; D008172:Lung Diseases, Fungal / Q000175:diagnosis* / Q000382:microbiology; D016533:Mycological Typing Techniques / Q000379:methods*; D011237:Predictive Value of Tests; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004271:DNA, Fungal