title: Fatty acids suppress autophagic turnover in β-cells.
abstract: Recent studies have shown that autophagy is essential for proper β-cell function and survival. However, it is yet unclear under what pathogenic conditions autophagy is inhibited in β-cells. Here, we report that long term exposure to fatty acids and glucose block autophagic flux in β-cells, contributing to their toxic effect. INS1 cells expressing GFP-LC3 (an autophagosome marker) were treated with 0.4 mm palmitate, 0.4 mm oleate, and various concentrations of glucose for 22 h. Kinetics of the effect of fatty acids on autophagy showed a biphasic response. During the second phase of autophagy, the size of autophagosomes and the content of autophagosome substrates (GFP-LC3, p62) and endogenous LC3 was increased. During the same phase, fatty acids suppressed autophagic degradation of long lived protein in both INS1 cells and islets. In INS1 cells, palmitate induced a 3-fold decrease in the number and the acidity of Acidic Vesicular Organelles. This decrease was associated with a suppression of hydrolase activity, suppression of endocytosis, and suppression of oxidative phosphorylation. The combination of fatty acids with glucose synergistically suppressed autophagic turnover, concomitantly suppressing insulin secretion. Rapamycin treatment resulted in partial reversal of the inhibition of autophagic flux, the inhibition of insulin secretion, and the increase in cell death. Our results indicate that excess nutrient could impair autophagy in the long term, hence contributing to nutrient-induced β-cell dysfunction. This may provide a novel mechanism that connects diet-induced obesity and diabetes.
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: Guy
authors.initials: G
authors.lastname: Las
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: Sam B
authors.initials: SB
authors.lastname: Serada
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: Jakob D
authors.initials: JD
authors.lastname: Wikstrom
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118.
authors.forename: Gilad
authors.initials: G
authors.lastname: Twig
authors.affiliation: Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118. Electronic address: Shirihai@bu.edu.
authors.forename: Orian S
authors.initials: OS
authors.lastname: Shirihai
mesh_terms: D000818:Animals; D001343:Autophagy; D002460:Cell Line; D002469:Cell Separation; D003920:Diabetes Mellitus / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D020123:Sirolimus / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D007328:Insulin; D049452:Green Fluorescent Proteins; D005947:Glucose; D020123:Sirolimus