title: Analysis of the expression of platelet antigens CD9 and CD41/61 in transgenic rabbits with the integrated human blood clotting factor VIII gene construct.
abstract: The objective of this research was to study the expression of cell membrane molecules CD9 and CD41/61 of transgenic rabbit with integrated human factor VIII (rhFVIII) gene construct. The expressions of these molecules have been monitored during two lactations of transgenic rabbits and simultaneously compared with the expression of the same molecules of non-transgenic rabbits. The immunochemical analysis by indirect immunofluorescence, ELISA and indirect immunoperoxidase staining of blood cells and udder tissues show that the insertion of the WAP-hFVIII gene construct into the rabbit genome, do not influence the expression of cell membrane antigens CD9 and CD41/61 on the blood platelets, polymorphonuclear blood cells, milk somatic cells and mammary gland tissues.
authors.affiliation: Institute of Animal Biochemistry and Genetics, Centre of Excellence of Slovak Research and Development Agency "Biomembranes 2008", Slovak Academy of Sciences, Moyzesova 61, 900 28 Ivanka pri Dunaji, Slovak Republic. Michal.Simon@savba.sk
authors.forename: Michal
authors.initials: M
authors.lastname: Simon
authors.forename: Jana
authors.initials: J
authors.lastname: Antalikova
authors.forename: Peter
authors.initials: P
authors.lastname: Chrenek
authors.forename: Lubica
authors.initials: L
authors.lastname: Horovska
authors.forename: Svatoslav
authors.initials: S
authors.lastname: Hluchy
authors.forename: Katarina
authors.initials: K
authors.lastname: Michalkova
authors.forename: Jana
authors.initials: J
authors.lastname: Jankovicova
authors.forename: Vladimir
authors.initials: V
authors.lastname: Tancin
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015703:Antigens, CD / Q000378:metabolism*; D001777:Blood Coagulation / Q000235:genetics; D001792:Blood Platelets / Q000378:metabolism*; D002460:Cell Line; D005169:Factor VIII / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D039661:Integrin beta3 / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism*; D011817:Rabbits; D060245:Tetraspanin 29
chemical_list: D015703:Antigens, CD; C554869:CD9 protein, human; D039661:Integrin beta3; D008562:Membrane Glycoproteins; D040201:Platelet Membrane Glycoprotein IIb; D060245:Tetraspanin 29; D005169:Factor VIII