title: RD antigen based nanovaccine imparts long term protection by inducing memory response against experimental murine tuberculosis.
abstract: BACKGROUND
  The absence of certain genomic loci that are present in most of the virulent strains of Mycobacterium tuberculosis as well as lack of lasting memory responses are some of the major causes attributed to the non effectiveness of Bacille Calmette-Gue'rin (BCG) vaccine. Immunization schedules addressing these issues can offer better strategy for protection against tuberculosis.
  
  
  METHODS
  The immunological responses evoked upon administration of archaeosome based antigen delivery system comprising T cell antigen, Rv3619c (an ESAT-6 family protein), has been assessed against experimental murine tuberculosis in BALB/c mice.
  
  
  RESULTS
  Archaeosome based subunit vaccine has been found to elicit type-1 cytokines in the immunized mice. Besides effective T cell memory response, the Rv3619c based vaccine was able to reduce mycobacterial burden in the animals challenged with Mycobacterium tuberculosis infection.
  
  
  CONCLUSIONS
  The data of the present study suggest that archaeosome encapsulated RD gene products offer substantial protection against M. tuberculosis infection.
authors.affiliation: Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
authors.forename: Mairaj Ahmed
authors.initials: MA
authors.lastname: Ansari
authors.forename: Swaleha
authors.initials: S
authors.lastname: Zubair
authors.forename: Anjum
authors.initials: A
authors.lastname: Mahmood
authors.forename: Pushpa
authors.initials: P
authors.lastname: Gupta
authors.forename: Aijaz A
authors.initials: AA
authors.lastname: Khan
authors.forename: Umesh D
authors.initials: UD
authors.lastname: Gupta
authors.forename: Ashish
authors.initials: A
authors.lastname: Arora
authors.forename: Mohammad
authors.initials: M
authors.lastname: Owais
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000276:immunology; D053758:Nanoparticles / Q000627:therapeutic use*; D018417:Th1 Cells / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014376:Tuberculosis / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D032581:Tuberculosis Vaccines / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D015415:Biomarkers; D016207:Cytokines; D007074:Immunoglobulin G; D032581:Tuberculosis Vaccines