title: Kinetics of effector functions and phenotype of virus-specific and γδ T lymphocytes in primary human cytomegalovirus infection during pregnancy.
abstract: The T-cell response to human cytomegalovirus (HCMV) primary infection was analyzed in 27 pregnant women during the first year after primary HCMV infection. Pregnant women with remote HCMV infection were enrolled as controls. Interferon-γ-producing T cells were readily detected at levels comparable (CD4(+)) or higher (CD8(+)) than controls, whereas the CD4(+) and CD8(+) lymphoproliferative response as well as IL-2 production was significantly reduced with respect to controls for at least 9 months after infection. In addition, CD45RA re-expression as well as cytotoxic T lymphocyte activity and perforin expression were the major components of the adaptive CD4(+) and CD8(+) T-cell immune response, while Vδ2(-) γδ T-cell expansion in response to HCMV infection followed kinetics similar to that of CD8(+) T cells. Reduced CD45RA re-expression directly correlated with HCMV transmission to the fetus, thus providing an important prognostic parameter.
authors.affiliation: Experimental Research Laboratories, Transplantation Area, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Fornara
authors.forename: Daniele
authors.initials: D
authors.lastname: Lilleri
authors.forename: M Grazia
authors.initials: MG
authors.lastname: Revello
authors.forename: Milena
authors.initials: M
authors.lastname: Furione
authors.forename: Maurizio
authors.initials: M
authors.lastname: Zavattoni
authors.forename: Elisa
authors.initials: E
authors.lastname: Lenta
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Gerna
mesh_terms: D056704:Adaptive Immunity; D000328:Adult; D049109:Cell Proliferation; D003587:Cytomegalovirus / Q000276:immunology* / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D018445:Infectious Disease Transmission, Vertical; D007371:Interferon-gamma / Q000378:metabolism; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D011264:Pregnancy Trimesters; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta; D007371:Interferon-gamma