title: Biochemical characterization of Sfh-I, a subclass B2 metallo-beta-lactamase from Serratia fonticola UTAD54.
abstract: The subclass B2 metallo-β-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-β-lactamase with some distinctive properties.
authors.affiliation: CESAM & Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal. ffonseca@ua.pt
authors.forename: Fátima
authors.initials: F
authors.lastname: Fonseca
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Arthur
authors.forename: Elizabeth H C
authors.initials: EH
authors.lastname: Bromley
authors.forename: Bart
authors.initials: B
authors.lastname: Samyn
authors.forename: Pablo
authors.initials: P
authors.lastname: Moerman
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Saavedra
authors.forename: António
authors.initials: A
authors.lastname: Correia
authors.forename: James
authors.initials: J
authors.lastname: Spencer
mesh_terms: D015780:Carbapenems / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D012705:Serratia / Q000201:enzymology*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015780:Carbapenems; D001618:beta-Lactamases