title: A new strategy for the synthesis of bisaminoacylated tRNAs.
abstract: Tandemly activated tRNAs participate effectively in protein synthesis and exhibit superior chemical and biochemical stability compared to the more commonly used singly aminoacylated tRNAs. While several bisaminoacylated tRNAs have been prepared via the T4 RNA ligase-mediated condensation of bisaminoacylated pdCpAs and abbreviated tRNA transcripts (tRNA-C(OH)), the bisaminoacylated pdCpAs are difficult to prepare when using bulky amino acids. Described herein is a new strategy for preparing bisaminoacylated tRNAs, applicable even for bulky amino acids.
authors.affiliation: Center for BioEnergetics, The Biodesign Institute, Arizona State University, Tempe, Arizona 85827, USA.
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Nangreave
authors.forename: Larisa M
authors.initials: LM
authors.lastname: Dedkova
authors.forename: Shengxi
authors.initials: S
authors.lastname: Chen
authors.forename: Sidney M
authors.initials: SM
authors.lastname: Hecht
mesh_terms: D000215:Acylation; D015394:Molecular Structure; D012343:RNA, Transfer / Q000138:chemical synthesis* / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D012343:RNA, Transfer