title: Identification of yeast isolated from dermatological patients by MALDI-TOF mass spectrometry.
abstract: Species identification of yeasts is based on biochemical (e.g. API ID 32 C®, bioMérieux) and molecular biological approaches. As an alternative to DNA-dependent methods, mass spectral analysis based identification of micro-organisms has become increasingly recognized. In a number of studies, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been applied for the rapid classification and identification of micro-organisms. In this study, the applicability of MALDI-TOF MS for identifying yeasts isolated from dermatological patients was analysed and compared with the results from the API ID 32 C® system. Furthermore, sequencing the internal transcribed spacer (ITS) regions of the ribosomal DNA was employed as reference method. Candida (C.) albicans was isolated in 41.9% of all cases, C. parapsilosis in 20.3%, C. glabrata in 10.8%, and C. krusei in 6, 8.1%. Rarely isolated yeasts were Candida colliculosa, famata, guilliermondii, lusitaniae, and tropicalis as well as Geotrichum candidum, Rhodotorula mucilaginosa and Trichosporon mucoides. The MALDI TOF results were equal to the results gained by ITS sequence analysis in 94%, whereas API ID 32 C® provided the correct diagnosis in 84.3% (of all cases). This lower identification rate is mostly referable to frequent misidentifications of C. krusei as C. inconspicua/norvegensis,Candida tropicalis, or Geotrichum capitatum. In contrast, all C. krusei strains were correctly identified by MALDI TOF MS. In conclusion, species identification by MALDI-TOF MS was proven to be consistent with ITS sequence analysis; the technique has a resolving power comparatively as high as ITS sequence analysis.
authors.affiliation: Klinik für Hautkrankheiten, Universitätsklinikum Jena, Jena, Germany. florian.seyfarth@med.uni-jena.de
authors.forename: Florian
authors.initials: F
authors.lastname: Seyfarth
authors.forename: Cornelia
authors.initials: C
authors.lastname: Wiegand
authors.forename: Marcel
authors.initials: M
authors.lastname: Erhard
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Gräser
authors.forename: Peter
authors.initials: P
authors.lastname: Elsner
authors.forename: Uta-Christina
authors.initials: UC
authors.lastname: Hipler
mesh_terms: D006801:Humans; D016533:Mycological Typing Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis / Q000382:microbiology*; D012871:Skin Diseases / Q000175:diagnosis / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015003:Yeasts / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*