title: Improved production of L-lysine by over-expression of Meso-diaminopimelate decarboxylase enzyme of Corynebacterium glutamicum in Escherichia coli.
abstract: The aim of this study is over-expression of Meso-diaminopimelate decarboxylase enzyme (EC 4.1.1.20) and enhancement of L-lysine production rate. The C. glutamicum LysA gene which encodes a Meso-diaminopimelate decarboxylase was cloned in E. coli. The cloned gene was sequenced; it encodes a 445 amino acids protein with molecular weight of 47 kD. Expression of the LysA gene in E. coli resulted in an increase in Meso-diaminopimelate decarboxylase activity, correlated with the presence in sodium dodecyl sulfate-polyacrylamid gels of a clear protein band that corresponds to this enzyme. The induction of cloned gene by IPTG has been shown to have an inhibitory effect on cell growth due to over-expression of the cloned gene. A two fold increase in lysine production rate was observed after introduction of the cloned gene into E. coli.
authors.affiliation: Department of Biochemistry, Payam-E-Noor University, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Cheraghi
authors.forename: A
authors.initials: A
authors.lastname: Akbarzade
authors.forename: A
authors.initials: A
authors.lastname: Farhangi
authors.forename: M
authors.initials: M
authors.lastname: Chiani
authors.forename: Z
authors.initials: Z
authors.lastname: Saffari
authors.forename: S
authors.initials: S
authors.lastname: Ghassemi
authors.forename: H
authors.initials: H
authors.lastname: Rastegari
authors.forename: M R
authors.initials: MR
authors.lastname: Mehrabi
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics; D001709:Biotechnology / Q000379:methods*; D002262:Carboxy-Lyases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D048230:Corynebacterium glutamicum / Q000201:enzymology* / Q000235:genetics; D004247:DNA / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008239:Lysine / Q000378:metabolism*; D008970:Molecular Weight; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D004247:DNA; D002262:Carboxy-Lyases; C079912:LysA protein, Bacteria; C020031:diaminopimelic acid decarboxylase; D008239:Lysine