title: Controlled gating and electrical detection of single 50S ribosomal subunits through a solid-state nanopore in a microfluidic chip.
abstract: We describe analysis and control of 50S ribosomal subunits by a solid-state 45nm diameter nanopore incorporated in a microfluidic chip. When used as a resistive pulse sensor, translocation of single 50S subunits through the nanopore produces current blockades that have a linear dependence on applied voltage. Introduction of individual subunits into the fluidic channel shows a threshold behavior that allows controlled entry of individual 50S ribosomal subunits. The incorporation of nanopores into a larger optofluidic chip system opens possibilities for electrical and optical studies of single ribosomes in well-defined and rapidly variable chemical environments.
authors.affiliation: School of Engineering, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Rudenko
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Holmes
authors.forename: Dmitri N
authors.initials: DN
authors.lastname: Ermolenko
authors.forename: Evan J
authors.initials: EJ
authors.lastname: Lunt
authors.forename: Sarah
authors.initials: S
authors.lastname: Gerhardt
authors.forename: Harry F
authors.initials: HF
authors.lastname: Noller
authors.forename: David W
authors.initials: DW
authors.lastname: Deamer
authors.forename: Aaron
authors.initials: A
authors.lastname: Hawkins
authors.forename: Holger
authors.initials: H
authors.lastname: Schmidt
mesh_terms: D055664:Electrochemical Techniques; D004867:Equipment Design; D004926:Escherichia coli / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D058608:Nanopores; D036103:Nanotechnology; D054681:Ribosome Subunits, Large, Bacterial / Q000737:chemistry*