title: Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: an in vitro study.
abstract: In this [corrected] study, we purified hcbCA I and II from human cord blood erythrocytes using [corrected] Sepharose-4B-l [corrected] tyrosine-sulfanilamide affinity gel chromatography. [corrected]. The inhibition effects of ampicillin sulfate, ceftriaxone, ceftizoxime and ranitidine on hcbCA I and hcbCA II were also monitored. [corrected]. IC(50) values for ceftriaxone, ceftizoxime and ranitidine were found to be 27.l, 79.4 and 55.5 µM, respectively, [corrected] for hcbCA I, and [corrected] 21.0, 79.1 and 66.1 µM, respectively, [corrected] for hcbCA II. [corrected]. According to these results, ampicillin [corrected] sulfate inhibited only hcbCAII and IC(50) value [corrected] of this antibiotic was found to be 56.8 µM. All [corrected] substances were found to be [corrected] non-competitive inhibitors. It is important to study the inhibition effects of these drugs on hcbCA I and II izoenzymes as pregnant women are often prescribed these antibiotics. [corrected]. For this reason, the dosage of [corrected] these drugs should be carefully evaluated [corrected] to minimize side effects.
authors.affiliation: Erzincan University, Science and Art Faculty, Biology Department, Erzincan, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Cankaya
authors.forename: Mehmet
authors.initials: M
authors.lastname: Aktas
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kuzucu
authors.forename: İlhami
authors.initials: İ
authors.lastname: Gül
authors.forename: T Abdulkadir
authors.initials: TA
authors.lastname: Coban
mesh_terms: D024401:Carbonic Anhydrase I / Q000037:antagonists & inhibitors*; D024402:Carbonic Anhydrase II / Q000037:antagonists & inhibitors*; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D005312:Fetal Blood / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II