title: A combined proteomic and immunologic approach for the analysis of Schistosoma mansoni cercariae and adult worm protein extracts and the detection of one of the vaccine candidates, Sm28GST, from a Venezuelan parasite isolate.
abstract: Understanding the mode of Schistosoma mansoni larval invasion and the mechanism of immune evasion utilized by larvae and adult worms is essential for a rational development of vaccines or drugs to prevent or cure the disease. This parasite has a very complex molecular organization in all parasite stages, and identifying the major parasite proteins would give clues to schistosome metabolism and to the interaction of the parasite with the host immune system. Our goal was the evaluation of the protein parasite repertoire using a proteomic approach, and the characterization of protein extracts from two different parasite stages of a Venezuelan isolate, such as cercariae and adult worms, previously performed by other authors in some other strains. A comparison among authors was made. Besides, we aimed to identify different isoforms of one of the vaccine candidates, the gluthation-S-transferase protein (Sm28GST), by 2D SDS-PAGE and mass spectrometry, and to achieve its immunologic detection using sera from rabbits immunized with synthetic peptides derived from the Sm28GST protein. These techniques allowed the identification of some of the target molecules of the protective immune response that are being evaluated as potential members of a multi-component and multi-stage anti-S. mansoni vaccine and to clarify if the selected peptides induce antibodies that are able to recognize different isoforms of the Sm28GST.
authors.affiliation: Seccion de Biohelmintiasis, Instituto de Medicina Tropical, Escuela de Medicina Luis Razetti, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela.
authors.forename: Sandra
authors.initials: S
authors.lastname: Losada
authors.forename: Laurence
authors.initials: L
authors.lastname: Sabatier
authors.forename: Philippe
authors.initials: P
authors.lastname: Hammann
authors.forename: Christelle
authors.initials: C
authors.lastname: Guillier
authors.forename: César
authors.initials: C
authors.lastname: Matos
authors.forename: Henry
authors.initials: H
authors.lastname: Bermúdez
authors.forename: María Angelita
authors.initials: MA
authors.lastname: Lorenzo
authors.forename: Oscar
authors.initials: O
authors.lastname: Noya
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D058487:Cercaria; D005982:Glutathione Transferase / Q000235:genetics* / Q000276:immunology*; D015801:Helminth Proteins / Q000235:genetics*; D040901:Proteomics; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology*; D014612:Vaccines; D014687:Venezuela
chemical_list: D000942:Antigens, Bacterial; D015801:Helminth Proteins; D014612:Vaccines; C484242:28-kilodalton glutathione S-transferase antigen, Schistosoma mansoni; D005982:Glutathione Transferase