title: Chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus Metarhizium robertsii.
abstract: A lack of selectable markers was a hindrance in investigating gene function in Metarhizium robertsii. A reliable Agrobacterium-mediated transformation system based on the use of chlorimuron ethyl as the selectable marker was developed which could serve as a useful tool to inactivate genes involved in insect pathogenicity.
authors.affiliation: State key laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Lin
authors.forename: Fengqing
authors.initials: F
authors.lastname: Wang
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wei
mesh_terms: D060054:Agrobacterium / Q000235:genetics; D000818:Animals; D020868:Gene Silencing; D007313:Insecta / Q000382:microbiology*; D052981:Metarhizium / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011743:Pyrimidines / Q000494:pharmacology*; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D014170:Transformation, Genetic
chemical_list: D011743:Pyrimidines; D013453:Sulfonylurea Compounds; C112343:chlorimuron ethyl