title: Sequencing and biological effects of an adipokinetic/hypertrehalosemic peptide in the stick insect, Baculum extradentatum.
abstract: The corpora cardiaca of the Vietnamese stick insect, Baculum extradentatum, contain a member of the adipokinetic hormone/red pigment-concentrating hormone/hypertrehalosemic hormone (AKH/RPCH/HrTH) family of peptides whose sequence is identical to that originally described for the Indian stick insect, Carausius morosus. This decapeptide, Carmo-HrTH-II (pELTFTPNWGTa), has both hypertrehalosemic and cardioacceleratory activity in B. extradentatum, and hyperlipaemic activity in locusts. Reversed-phase high performance liquid chromatography (RP-HPLC) of corpora cardiaca extract followed by MALDI-TOF MS/MS also revealed a novel modification of a second peptide in B. extradentatum: the tryptophan residue at position 8 is post-translationally modified to kynurenine.
authors.affiliation: Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Malik
authors.forename: Gerd
authors.initials: G
authors.lastname: Gäde
authors.forename: Angela B
authors.initials: AB
authors.lastname: Lange
mesh_terms: D000818:Animals; D056148:Chromatography, Reverse-Phase; D006321:Heart / Q000187:drug effects; D007301:Insect Hormones / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D007737:Kynurenine / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D048188:Locusta migratoria / Q000187:drug effects; D008297:Male; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry
chemical_list: D007301:Insect Hormones; D009479:Neuropeptides; D009842:Oligopeptides; C044833:adipokinetic hormone; C084112:hypertrehalosemic hormone; D007737:Kynurenine; D014364:Tryptophan; D011761:Pyrrolidonecarboxylic Acid