title: The tunica adventitia of human arteries and veins as a source of mesenchymal stem cells.
abstract: We previously demonstrated that human pericytes, which encircle capillaries and microvessels, give rise in culture to genuine mesenchymal stem cells (MSCs). This raised the question as to whether all MSC are derived from pericytes. Pericytes and other cells defined on differential expression of CD34, CD31, and CD146 were sorted from the stromal vascular fraction of human white adipose tissue. Besides pericytes, CD34+ CD31- CD146- CD45- cells, which reside in the outmost layer of blood vessels, the tunica adventitia, natively expressed MSC markers and gave rise in culture to clonogenic multipotent progenitors identical to standard bone marrow-derived MSC. Despite common MSC features and developmental properties, adventitial cells and pericytes retain distinct phenotypes and genotypes through culture. However, in the presence of growth factors involved in vascular remodeling, adventitial cells acquire a pericytes-like phenotype. In conclusion, we demonstrate the co-existence of 2 separate perivascular MSC progenitors: pericytes in capillaries and microvessels and adventitial cells around larger vessels.
authors.affiliation: Department of Orthopaedic Surgery, Orthopaedic Hospital Research Center, UCLA and Orthopaedic Hospital, Los Angeles, California 90095, USA.
authors.forename: Mirko
authors.initials: M
authors.lastname: Corselli
authors.forename: Chien-Wen
authors.initials: CW
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Solomon
authors.initials: S
authors.lastname: Yap
authors.forename: J Peter
authors.initials: JP
authors.lastname: Rubin
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D018952:Antigens, CD34 / Q000378:metabolism; D001158:Arteries / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity / Q000187:drug effects; D020286:Pericytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014680:Veins / Q000166:cytology*
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D051929:CD146 Antigen; D036341:Intercellular Signaling Peptides and Proteins