title: [Distribution of microbes and their hereditary diversity in the rhizosphere of carnation in greenhouse].
abstract: OBJECTIVE
  To investigate the relationship between microbial community and the blight diseases in rhizosphere of carnation for biological control.
  
  
  METHODS
  Bacterial strains isolated from the rhizosphere of healthy and blight carnation plants in greenhouse were replicated by morphology and 16S rRNA gene similarity, and investigated by using phylogenetic analysis based on 16S rRNA gene sequence alignments.
  
  
  RESULTS
  Isolates belonged to 4 phyla of bacteria, 65 isolates from the samples of healthy carnation plants belonged to 9 genera and Bacillus, Streptomyces, Mortierella were the dominant bacteria. However, 33 isolates from the samples of blight carnation plants belonged to 12 genera and Stenotrophomonas, Sphingobacterium, Pseudomonas, Chryseobacterium, Amycolatopsis and Fusarium were only isolated from the sample of blight carnation plants. At least 13 isolates should represent potential novel species based on lower similarities of 16 S rRNA gene (90%-98% ).
  
  
  CONCLUSIONS
  The result showed that either the percentage of the fungus in the total strains or the abundance of Bacillus groups in the total strains can be the referential targets to evaluate whether the carnation soil would be healthy for the carnational growth or lead to fusarium wilt diseases, accurately forecasting potential risks of the disease.
authors.affiliation: Energy and Environment Science college, Yunnan Normal University, Kunming 650092, China. xhgao-ynu@tom.com
authors.forename: Xuhong
authors.initials: X
authors.lastname: Gao
authors.forename: Songtao
authors.initials: S
authors.lastname: He
authors.forename: Huilin
authors.initials: H
authors.lastname: Guan
authors.forename: Shukun
authors.initials: S
authors.lastname: Tang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D029749:Dianthus / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S