title: p52 Activation in monomorphic B-cell posttransplant lymphoproliferative disorder/diffuse large B-cell lymphoma without BAFF-R expression.
abstract: Posttransplantation lymphoproliferative disorders (PTLD) are associated with Epstein-Barr virus (EBV) and activate the NF-κB pathway. B-cell activating factor (BAFF) modulates cell growth and survival in non-Hodgkin's lymphomas. However, there are few studies of EBV, BAFF/BAFF-R signaling, and NF-κB1 and NF-κB2 pathway activation in PTLD. Diffuse large B-cell lymphomas (DLBCL) in two different clinical contexts, immunocompetent patients (DLBCL/IC; n = 30) or posttransplantation solid-organ recipients (DLBCL/PTLD; n = 21), were characterized histogenically as germinal center (GC) or non-germinal center (NGC). Expression of BAFF, BAFF-R, and NF-κB proteins p50 and p52 and the presence or absence of EBV were compared in these clinical contexts. Regardless of the GC or NGC pattern of DLBCL, BAFF-R was expressed in 37% of DLBCL/IC but in only 4.8% of DLBCL/PTLD. p52 was expressed in DLBCL/PTLD/NGC (12 of 19 cases) as compared with DLBCL/IC/NGC (0 of 18 cases). This pattern might be related to the presence of EBV and latent membrane protein 1 because p52 expression was observed primarily in EBV-positive DLBCL/PTLD cases expressing latent membrane protein 1. Thus, the activation profile or NGC pattern of DLBCL/PTLD was not associated with BAFF/BAFF-R expression, whereas nuclear p52 related to NF-κB2 pathway activation might be linked to EBV.
authors.affiliation: Service d'Hématologie et Immunologie Biologiques, Cytogénétique, Villejuif, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Proust
authors.forename: Patricia
authors.initials: P
authors.lastname: Rincé
authors.forename: Rita
authors.initials: R
authors.lastname: Creidy
authors.forename: Thierry
authors.initials: T
authors.lastname: Lazure
authors.forename: Irène
authors.initials: I
authors.lastname: Joab
authors.forename: Loïc
authors.initials: L
authors.lastname: Garçon
authors.forename: Monique
authors.initials: M
authors.lastname: Fabre
authors.forename: Catherine
authors.initials: C
authors.lastname: Guettier
authors.forename: Martine
authors.initials: M
authors.lastname: Raphael
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D053264:B-Cell Activating Factor / Q000378:metabolism; D053265:B-Cell Activation Factor Receptor / Q000378:metabolism*; D002675:Child, Preschool; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D016403:Lymphoma, Large B-Cell, Diffuse / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D052003:NF-kappa B p52 Subunit / Q000378:metabolism*; D015398:Signal Transduction; D014180:Transplantation / Q000009:adverse effects*
chemical_list: D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; D052003:NF-kappa B p52 Subunit; C435926:TNFRSF13C protein, human; C505719:TNFSF13B protein, human