title: Determination of perfluorooctanoate and perfluorooctanesulfonate in water matrices by inline matrix elimination liquid chromatography with reversed phase separation and suppressed conductivity detection.
abstract: This work describes a new method for the determination of perfluorooctanoate (PFOA) and perfluorooctane sulfonate (PFOS) in water matrices by suppressed conductivity detection. Separation was achieved by isocratic elution on a reversed-phase column thermostated at 45°C using an aqueous mobile phase containing boric acid and acetonitrile. The PFOA and PFOS content in the water matrix were quantified by a pre-concentration technique. For the concentration range of 1 to 15 ng/mL and 2 to 30 ng/mL, the linear calibration curve for PFOA and PFOS yielded coefficients of determination (R(2)) of 0.9995 and 0.9985, respectively. The relative standard deviations were smaller than 1.5% for PFOA and PFOS. The retention-time precision of four consecutive 12 h injections was smaller than 0.641% and 0.818%, respectively. The presence of common divalent cations, such as calcium, magnesium, and iron in water matrices impairs PFOS recovery. This drawback was overcome by applying inline matrix elimination method. The optimized method was successfully applied for drinking water, ground water, and seawater samples.
authors.affiliation: Metrohm India Limited, 13, First Avenue, Indira Nagar, Adyar, Chennai 600 020, Tamilnadu, India. harisenthil@hotmail.com
authors.forename: N Harihara
authors.initials: NH
authors.lastname: Subramanian
authors.forename: P
authors.initials: P
authors.lastname: Manigandan
authors.forename: Andrea
authors.initials: A
authors.lastname: Wille
authors.forename: Ganga
authors.initials: G
authors.lastname: Radhakrishnan
mesh_terms: D017738:Alkanesulfonic Acids / Q000032:analysis*; D002210:Caprylates / Q000032:analysis*; D002412:Cations / Q000032:analysis; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D004553:Electric Conductivity; D005466:Fluorocarbons / Q000032:analysis*; D005618:Fresh Water; D016018:Least-Squares Analysis; D015203:Reproducibility of Results; D012623:Seawater; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply
chemical_list: D017738:Alkanesulfonic Acids; D002210:Caprylates; D002412:Cations; D005466:Fluorocarbons; D014874:Water Pollutants, Chemical; D014867:Water; C023036:perfluorooctanoic acid; C076994:perfluorooctane sulfonic acid