title: Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa.
abstract: The objective of the present study was to investigate the effect of Single-Layer Centrifugation (SLC) on boar spermatozoa, namely the effect of removal of seminal plasma proteins and cholesterol from the surface of spermatozoa. The presence of porcine seminal plasma proteins I and II (PSP-I/PSP-II) before and after SLC was studied using immunofluorescence, whereas the removal of cholesterol was shown qualitatively by thin-layer chromatography (TLC). Finally, the integrity of the sperm plasma membrane was observed by electron microscopy. It was shown that the seminal plasma proteins PSP-I and -II were removed from spermatozoa during SLC but could be restored by adding seminal plasma to the SLC-selected sperm samples. Some cholesterol was also lost from the spermatozoa during SLC but the plasma membrane itself appeared to be morphologically intact. Further studies are underway to examine the relevance of these findings to boar sperm cryopreservation and sperm fertility.
authors.affiliation: Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Kruse
authors.forename: P C
authors.initials: PC
authors.lastname: Dutta
authors.forename: J M
authors.initials: JM
authors.lastname: Morrell
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D002469:Cell Separation / Q000662:veterinary; D002498:Centrifugation / Q000009:adverse effects / Q000379:methods / Q000662:veterinary*; D002784:Cholesterol / Q000378:metabolism*; D003102:Colloids / Q000009:adverse effects; D007202:Indicators and Reagents / Q000009:adverse effects / Q000737:chemistry*; D008297:Male; D012661:Semen / Q000166:cytology / Q000378:metabolism; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D029607:Seminal Vesicle Secretory Proteins / Q000378:metabolism; D012821:Silanes / Q000009:adverse effects / Q000737:chemistry; D012822:Silicon Dioxide / Q000009:adverse effects / Q000737:chemistry; D053625:Sperm Retrieval / Q000662:veterinary; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D013499:Surface Properties; D034421:Sus scrofa
chemical_list: C403136:3-glycidoxypropyltrimethoxysilane; D003102:Colloids; D007202:Indicators and Reagents; D029589:Seminal Plasma Proteins; D029607:Seminal Vesicle Secretory Proteins; D012821:Silanes; C074521:seminal vesicle secretory protein II, porcine; C074520:seminal vesicle secretory protein 109, porcine; D012822:Silicon Dioxide; D002784:Cholesterol