title: Nonlinear response of gene expression to chemical perturbations: a noise-detector model and its predictions.
abstract: The widespread use of microarrays provided a first glimpse at some simple laws and organizing principles that govern the transcriptome. Previous analyses have shown that the transcriptional organization is very heterogeneous and characterized by a power-law decay for gene expression levels. Moreover, a simple law was unveiled suggesting that gene expression dynamic changes under stress are proportional to their initial expression values. However, to elucidate and assess the underlying governing principles of transcriptional organization, we do not only need to identify them, but also provide theoretical models that are able to faithfully capture and reproduce them. Here we present a method to investigate the gene expression dynamics inspired by the theory of nonlinear transformation of random signals and noise. The model is able to explain not only the well-known power-law decay for abundance of expression levels, but also to reproduce the linear dependence of the standard deviation of gene expression change with respect to the initial expression value (also known as rich-travels-more dynamics). To our knowledge, this is the first model applied to gene expression dynamics that is able to simultaneously predict both statistical features. The theoretical framework derives an indicator to measure the coupling between gene expression and specific perturbations. Using genome-wide transcriptional data, this indicator identifies genes strongly coupled to specific inflammatory responses to different pathogens. The novel application of signal and noise theory to study intracellular responses and gene expression changes offers not only a new theoretical avenue to study transcriptional responses to environmental stresses and chemical signals but also provides predictive capability at the genome scale.
authors.affiliation: Department of Complex and Intelligent Systems, Future University Hakodate, Kamedanakano-cho, Hokkaido, Japan. nacher@fun.ac.jp
authors.forename: Jose C
authors.initials: JC
authors.lastname: Nacher
authors.forename: Vladimir B
authors.initials: VB
authors.lastname: Ryabov
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012680:Sensitivity and Specificity; D013997:Time Factors; D051193:Toll-Like Receptors / Q000819:agonists / Q000378:metabolism; D059467:Transcriptome
chemical_list: D051193:Toll-Like Receptors