title: Methodology: a modified method for high-quality DNA extraction for molecular analysis in cereal plants.
abstract: Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high-quality genomic DNA from leaves and seeds of major cereal crops with minor modifications. DNA yields ranged from 300 to 1800 ng for 0.01 g seed or leaf tissue.
authors.affiliation: Department of Biochemistry, Quaid-e-Azam University, Islamabad, Pakistan. zahidahasan82pk@hotmail.com
authors.forename: Z H
authors.initials: ZH
authors.lastname: Pervaiz
authors.forename: N A
authors.initials: NA
authors.lastname: Turi
authors.forename: I
authors.initials: I
authors.lastname: Khaliq
authors.forename: M A
authors.initials: MA
authors.lastname: Rabbani
authors.forename: S A
authors.initials: SA
authors.lastname: Malik
mesh_terms: D018744:DNA, Plant / Q000302:isolation & purification*; D002523:Edible Grain / Q000235:genetics*; D018745:Genome, Plant; D018515:Plant Leaves / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D012639:Seeds / Q000737:chemistry
chemical_list: D018744:DNA, Plant