title: Role of single-dose clostridiopeptidase A collagenase in peritoneal adhesions.
abstract: BACKGROUND
  To evaluate the effect of clostridiopeptidase A collagenase (CAC) in a rat model of postoperative peritoneal adhesion (PPA).
  
  
  METHODS
  Forty rats were divided into four equal groups. In group 1, 1 g CAC was injected into the peritoneal cavity. In group 2, adhesions were generated. In group 3, adhesions were generated, and, 10 days later, the area with PPAs was covered with 1 g CAC. In group 4, adhesions were generated and the area was covered immediately with 1 g CAC. All rats were sacrificed on postoperative day 10, and adhesions were scored both macroscopically and microscopically.
  
  
  RESULTS
  Mean macroscopic adhesion scores in groups 1-4 were 0, 2.9 ± 0.21, 2.55 ± 0.16, and 1.3 ± 1, respectively (p = 0.0001). The mean macroscopic adhesion score in group 4 was lower than that in group 2 (p = 0.045). The mean macroscopic adhesion score in group 4 was lower than that in group 3, but the value was not statistically different (p = 0.098). Mean microscopic values were 0, 2.8 ± 0.42, 2.5 ± 0.52, and 1.3 ± 0.67, respectively (p< 0.0001). The group 4 score was lower than those of groups 2 (p = 0.01) and 3 (p = 0.025).
  
  
  CONCLUSIONS
  A single dose of CAC reduces PPA formation. CAC, however, is not effective on already formed PPAs.
authors.affiliation: Bezmialem Vakif University, Istanbul, Turkey. dr@webcerrah.com
authors.forename: E
authors.initials: E
authors.lastname: Aysan
authors.forename: H
authors.initials: H
authors.lastname: Bektas
authors.forename: F
authors.initials: F
authors.lastname: Ersoz
authors.forename: K
authors.initials: K
authors.lastname: Behzat
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D003012:Microbial Collagenase / Q000008:administration & dosage*; D010532:Peritoneal Diseases / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control*
chemical_list: D003094:Collagen; D003012:Microbial Collagenase