title: Nano polyurethane-assisted ultrasensitive biodetection of H(2)O(2) over immobilized microperoxidase-11.
abstract: Nanostructured polyurethane (PU) synthesized by an emulsion polymerization with narrow size distribution was employed for the first time directly as a novel matrix for enzyme immobilization to develop sensitively amperometric biosensors. When Microperoxidase-11 (MP-11) was selected as a model protein, the resulting hydrogen peroxide (H(2)O(2)) biosensor exhibited improved sensitivity of 29.6μAmM(-1)cm(-2) with quite good response time of (1.3±0.4)s and remarkable limit of detection as low as 10pM (S/N 3) over existing protocols. A linear calibration curve for hydrogen peroxide was obtained up to 1.3μM under the optimized conditions with a relative low calculated Michaelis-Menten constant (K(M)(app)) (1.87±0.05)μM, which indicated the enhanced enzymatic affinity of MP-11 to H(2)O(2) via PU. The possible interferents had negligible effect on the response current and time of the prepared biosensor. Results suggest that the PU nanoparticles (PU-NPs) with good biocompatibility and sufficient interfacial adhesion hold promise as an attractive support material for construction of ultrasensitive amperometric biosensor.
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, PR China.
authors.forename: Zhenping
authors.initials: Z
authors.lastname: Chen
authors.forename: Dongmei
authors.initials: D
authors.lastname: Sun
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jiayue
authors.initials: J
authors.lastname: Zhao
authors.forename: Tianhong
authors.initials: T
authors.lastname: Lu
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Huang
authors.forename: Chenxin
authors.initials: C
authors.lastname: Cai
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010544:Peroxidases; D011140:Polyurethanes
chemical_list: D004800:Enzymes, Immobilized; D011140:Polyurethanes; D006861:Hydrogen Peroxide; D010544:Peroxidases; C021799:microperoxidase