title: Prevalence and multiplicity of HPV in HIV women in Minas Gerais, Brazil.
abstract: OBJECTIVE
  To detect the frequency and subtypes of HPV in the uterine cervix of HIV-infected women.
  
  
  METHODS
  Sample consisted of 288 HIV-infected women, recruited from the public health system of five cities of Minas Gerais, Brazil. Women were seen from August 2003 to August 2008. Cervical samples were collected for cytological analysis and for HPV DNA detection, using polymerase chain reaction (PCR). HPV DNA was classified according to its oncogenic potential in low risk (types 6, 11) and high risk (types 16, 18, 31, 33, 35). Colposcopy was performed, followed by cervical biopsy when necessary. Categorical variables were compared using the Chi-squared test, with a significance level established at the 5% level.
  
  
  RESULTS
  HPV prevalence was 78.8%. Most frequent genotypes were HPV-6 (63.9%) and HPV-16 (48.5%). High-risk HPV were observed in 70.5% of the women; low-risk in 71.4%; both high and low-risk HPV were detected in 55.1% of the patients. Multiple HPV genotypes were detected in 64.8% of the patients; two genotypes in 23.8%, and three in 18.9%.
  
  
  CONCLUSIONS
  HPV prevalence was high among HIV-infected women. Multiple HPV genotypes were common in samples from the uterine cervix of HIV-infected women.
authors.affiliation: Medical School, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Christine Miranda
authors.initials: CM
authors.lastname: Corrêa
authors.forename: Nara Chartuni Pereira
authors.initials: NC
authors.lastname: Teixeira
authors.forename: Angela Cristina Labanca de
authors.initials: AC
authors.lastname: Araújo
authors.forename: Nara de Oliveira
authors.initials: Nde O
authors.lastname: Carvalho
authors.forename: Dora Mendez Del
authors.initials: DM
authors.lastname: Castillo
authors.forename: Rachel Rezende
authors.initials: RR
authors.lastname: Campos
authors.forename: Isaura Vargas
authors.initials: IV
authors.lastname: Oliveira
authors.forename: Antonio Rafael
authors.initials: AR
authors.lastname: Alves
authors.forename: Ana Flávia de Moura
authors.initials: AF
authors.lastname: França
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Melo
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus / Q000235:genetics; D001938:Brazil / Q000453:epidemiology; D018791:CD4 Lymphocyte Count; D002584:Cervix Uteri / Q000821:virology*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral