title: Combined approach to demonstrate acetylcholinesterase activity changes in the rat brain following tabun intoxication and its treatment.
abstract: Reactivation effects of K203 and currently available oximes (obidoxime, HI-6) in combination with atropine on acetylcholinesterase activities in the brain parts of rats poisoned with tabun were studied. The activity was determined by quantitative histochemical and biochemical methods correlating between them very well. The tabun-induced changes in acetylcholinsterase activity as well as in reactivation potency of reactivators used were different in various parts of the brain. Pontomedullar area seems to be important for observed changes following tabun intoxication and its treatment. From the oximes studied, the reactivation effect of K203 was comparable with obidoxime; HI-6 was ineffective. Combination of bio- and histochemical methods allow fine differentiation among the action of different oximes following tabun poisoning.
authors.affiliation: Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic. bajgar@pmfhk.cz
authors.forename: Jiri
authors.initials: J
authors.lastname: Bajgar
authors.forename: Petr
authors.initials: P
authors.lastname: Hajek
authors.forename: Jiri
authors.initials: J
authors.lastname: Kassa
authors.forename: Dasa
authors.initials: D
authors.lastname: Slizova
authors.forename: Otakar
authors.initials: O
authors.lastname: Krs
authors.forename: Jana Zdarova
authors.initials: JZ
authors.lastname: Karasova
authors.forename: Josef
authors.initials: J
authors.lastname: Fusek
authors.forename: Lukas
authors.initials: L
authors.lastname: Capek
authors.forename: Victor A
authors.initials: VA
authors.lastname: Voicu
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D001931:Brain Mapping; D002619:Chemical Warfare Agents / Q000506:poisoning*; D002801:Cholinesterase Reactivators / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002802:Cholinesterases / Q000378:metabolism*; D005260:Female; D015394:Molecular Structure; D020258:Neurotoxicity Syndromes / Q000201:enzymology / Q000473:pathology / Q000517:prevention & control; D062025:Organophosphate Poisoning; D010755:Organophosphates; D010091:Oximes / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D011726:Pyridinium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: C524716:1-(4-carbamoylpyridinium)-4-(4-hydroxyiminomethylpyridinium)but-2-ene; D002619:Chemical Warfare Agents; D002801:Cholinesterase Reactivators; D010755:Organophosphates; D010091:Oximes; D011726:Pyridinium Compounds; D002802:Cholinesterases; C009374:tabun