title: Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue.
abstract: A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventional Coomassie Brilliant Blue G staining with phosphoric acid in the staining solution. In addition, the dye stain does not contain any amount of acid and methanol, such as phosphoric acid. Considering the speed, simplicity, and low cost, the dye stain may be of more practical value than other dye-based protein stains in routine proteomic research.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Dong
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Jun-He
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D012394:Rosaniline Dyes / Q000737:chemistry*
chemical_list: D012394:Rosaniline Dyes; C048139:Coomassie blue; C004692:coomassie Brilliant Blue