title: Initial fibroblast attachment to Erbium:YAG laser-irradiated dentine.
abstract: OBJECTIVE
  To evaluate the effects of Erbium (Er):YAG laser irradiation on the morphology of resected dentine surfaces, and to investigate fibroblast attachment to laser-irradiated dentine surfaces.
  
  
  METHODS
  Dentine blocks obtained from single-rooted human teeth were divided into the following groups after sterilization in an autoclave: (i) Laser group treated with Er:YAG laser irradiation (30 mJ per pulse, 10 pps, 60 s); (ii) L-MTAD group treated with laser irradiation as in (i) plus a mixture of doxycycline, tetracycline isomer and citric acid; (iii) RC-Prep group treated with EDTA gel or cream (RC-Prep) and (iv) Control group left untreated. After each treatment, the dentine blocks were incubated with NIH/3T3 fibroblasts cultured to subconfluency in Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum and antibiotics. The number of attached cells amongst the groups was analysed statistically at the 5% significance level. The dentine surface morphologies and cell attachments were evaluated by counting assays, histological observations and scanning electron microscopy (SEM).
  
  
  RESULTS
  The number of attached cells was significantly higher (P < 0.05) in the Laser group than in the RC-Prep and Control groups at 16 h. Dendritic cell extension of the fibroblasts was only observed in the Laser group at 8 h by SEM. In the histological analyses, significantly more attached cells were found on the dentine surfaces treated with laser irradiation.
  
  
  CONCLUSIONS
  Er:YAG laser irradiation induced morphological alterations in dentine surfaces, which may improve the attachment of fibroblasts to dentine.
authors.affiliation: Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: G
authors.initials: G
authors.lastname: Bolortuya
authors.forename: A
authors.initials: A
authors.lastname: Ebihara
authors.forename: S
authors.initials: S
authors.lastname: Ichinose
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Anjo
authors.forename: C
authors.initials: C
authors.lastname: Kokuzawa
authors.forename: H
authors.initials: H
authors.lastname: Saegusa
authors.forename: N
authors.initials: N
authors.lastname: Kawashima
authors.forename: H
authors.initials: H
authors.lastname: Suda
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D048430:Cell Shape; D002614:Chelating Agents / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003804:Dentin / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure; D004318:Doxycycline / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010545:Peroxides / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D011829:Radiation Dosage; D012388:Root Canal Irrigants / Q000494:pharmacology; D013997:Time Factors; D014508:Urea / Q000494:pharmacology; D014885:Waxes / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; C475043:MTAD (intracanal irrigant); D010545:Peroxides; D011136:Polysorbates; D012388:Root Canal Irrigants; D014885:Waxes; D019343:Citric Acid; C025081:RC-Prep; D014508:Urea; D004492:Edetic Acid; D004318:Doxycycline