title: Cryphonectria naterciae: a new species in the Cryphonectria-Endothia complex and diagnostic molecular markers based on microsatellite-primed PCR.
abstract: In a recent study intended to assess the distribution of Cryphonectria parasitica in Portugal, 22 morphologically atypical orange isolates were collected in the Midwestern regions. Eleven isolates were recovered from Castanea sativa, in areas severely affected by chestnut blight and eleven isolates from Quercus suber in areas with cork oak decline. These isolates were compared with known C. parasitica and Cryphonectria radicalis isolates using an integrated approach comprising morphological and molecular methods. Morphologically the atypical isolates were more similar to C. radicalis than to C. parasitica. Phylogenetic analyses based on internal transcribed spacer (ITS) and β-tubulin sequence data grouped the isolates in a well-supported clade separate from C. radicalis. Combining morphological, cultural, and molecular data Cryphonectria naterciae is newly described in the Cryphonectria-Endothia complex. Microsatellite-primed PCR fingerprinting with (GACA)(4) primer discriminated between C. naterciae, C. radicalis, and C. parasitica.
authors.affiliation: Instituto Nacional de Recursos Biológicos, IP., Edifício da ex. Estação Florestal Nacional, Quinta do Marquês, Oeiras, Portugal. helena.braganca@inrb.pt
authors.forename: Helena
authors.initials: H
authors.lastname: Bragança
authors.forename: Daniel
authors.initials: D
authors.lastname: Rigling
authors.forename: Eugénio
authors.initials: E
authors.lastname: Diogo
authors.forename: Jorge
authors.initials: J
authors.lastname: Capelo
authors.forename: Alan
authors.initials: A
authors.lastname: Phillips
authors.forename: Rogério
authors.initials: R
authors.lastname: Tenreiro
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D029962:Fagaceae / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques / Q000379:methods*; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D029963:Quercus / Q000382:microbiology; D014404:Tubulin / Q000235:genetics
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D014404:Tubulin