title: Phytochemical and in vitro screening of some Ficus and Morus spp. for hypolipidaemic and antioxidant activities and in vivo assessment of Ficus mysorensis (Roth).
abstract: Phytochemical screening of air-dried leaves and fruit juice of certain Ficus and Morus spp. have been studied. In an in vitro study, the ethanol and hexane extracts of the investigated plants were evaluated against hyperlipidaemia by estimating the rate limiting enzyme of cholesterol biothenysis; β-hydroxy-β-methylglutaryl coenzyme A reductase (HMG-CoA reductase). The antioxidant activity was evaluated by reduction of DPPH(-) free radical. Extra phytochemical screening of Ficus extracts was undertaken, which recorded potent hypolipidaemic and antioxidant activities. The more pronounced extract, Ficus mysorensis (hexane extract), was evaluated in vivo by estimation of the lipid profile and certain antioxidant parameters in hypercholesterolemic rats. The hexane fraction was chromatographed and six isolated compounds were identified. Furthermore, its saponifiable fraction was identified by a MS/MS technique. In conclusion, F. mysorensis recorded hypolipidaemic and antioxidant effects. Detailed studies of the isolated compounds must be undertaken for an evaluation against hypercholesterolemia and free radical elevation.
authors.affiliation: Pharmacognosy Department , National Research Center, Dokki, Cairo, Egypt.
authors.forename: Nagwa E
authors.initials: NE
authors.lastname: Awad
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Seida
authors.forename: Manal A
authors.initials: MA
authors.lastname: Hamed
authors.forename: Ahlam H
authors.initials: AH
authors.lastname: Mahmoud
authors.forename: Marwa M
authors.initials: MM
authors.lastname: Elbatanony
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D030681:Ficus / Q000737:chemistry*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D013058:Mass Spectrometry; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013055:Spectrophotometry, Infrared
chemical_list: D000975:Antioxidants; D000960:Hypolipidemic Agents; D010936:Plant Extracts