title: Effects of organic contaminants in reactive oxygen species, protein carbonylation and DNA damage on digestive gland and haemolymph of land snails.
abstract: The present study focused on early responses of land snails Eobania vermiculata to organic environmental contaminants, by investigating the use of a newly-established method for the measurement of protein carbonylation as a new biomarker of terrestrial pollution, as well as by measuring the ROS production and the DNA damage. Land snails were exposed to different concentrations of chlorpyrifos, parathion-methyl or PAHs in vivo or in vitro in the laboratory. The susceptibility of exposed snails was increased in relation to oxidative stress induced by contaminants tested. A statistically significant increase in ROS production, protein carbonylation and DNA damage was revealed in the snails treated with pollutants, compared to the untreated ones. The results indicated the effectiveness of measuring ROS production and DNA damage and reinforce the application of the present ELISA method in organic terrestrial pollution biomonitoring studies.
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Itziou
authors.forename: M
authors.initials: M
authors.lastname: Kaloyianni
authors.forename: V K
authors.initials: VK
authors.lastname: Dimitriadis
mesh_terms: D000818:Animals; D001681:Biological Assay; D015415:Biomarkers / Q000378:metabolism; D004249:DNA Damage; D004064:Digestive System / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D054750:Ecotoxicology; D004785:Environmental Pollutants / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D006458:Hemolymph / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009930:Organic Chemicals / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D050050:Protein Carbonylation / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012908:Snails / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D009930:Organic Chemicals; D017382:Reactive Oxygen Species