title: The possible role of the PK1 and its receptor in the etiology of the preeclampsia.
abstract: OBJECTIVE
  Preeclampsia (PE) is a hypertensive complication of the pregnancy. In our study we investigated the expression, localization, and signaling pathways of PK1 and PKR1 in third-trimester human placenta and myometrium and assessed the correlation between the PK1 and PKR1 expression and signaling and the incidence of the PE.
  
  
  METHODS
  We designed two study groups: pregnant PE patients and healthy, pregnant women. After collection, tissue was placed in RNAlater for RNA extraction, fixed in 4% neutral buffered formalin, and wax embedded for immunohistochemistry or placed in RPMI and transported to the laboratory for in vitro culture. We have collected and processed placental and myometrial biopsies from 40 patients - 19 were PE patients.
  
  
  RESULTS
  Only the PK1 mRNA expression comparison between PE and CTRL in placenta showed statistically significant difference (p=0.004). There was statistically significant difference in cell signaling in myometrium controls in 30 minutes after ligand. The rise in pERK/tERK ratio is clearly visible in time intervals 20 and 30 minutes in controls, although with no statistical significance. There was no difference in PK1 and PKR1 localization in the placenta and myometrium in the groups.
  
  
  CONCLUSIONS
  The number of PKR1 is not reduced in PE. The reduced PK1 mRNA expression is not than dependent on PKR1 mRNA expression. The data shows that ST produces much more PK1 in healthy pregnant women than those suffering from PE. We can conclude that in PE the production of PK1 is impaired and so are the endocrine functions of the ST.
authors.affiliation: Department of Perinatology and Gynecology, Poznan University of Medical Sciences, Poznan, Poland. chejron74@wp.pl
authors.forename: Michael
authors.initials: M
authors.lastname: Szuber
authors.forename: Wieslaw
authors.initials: W
authors.lastname: Markwitz
authors.forename: Mariola
authors.initials: M
authors.lastname: Ropacka
authors.forename: Grzegorz H
authors.initials: GH
authors.lastname: Breborowicz
mesh_terms: D005260:Female; D005768:Gastrointestinal Hormones / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D009215:Myometrium / Q000502:physiology; D010920:Placenta / Q000502:physiology; D011225:Pre-Eclampsia / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / Q000235:genetics* / Q000378:metabolism
chemical_list: D005768:Gastrointestinal Hormones; C467610:PROK1 protein, human; C470837:PROKR1 protein, human; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived