title: The effects of electrical hippocampal kindling of seizures on amino acids and kynurenic acid concentrations in brain structures.
abstract: Our study demonstrated that the development of seizures during the electrically induced kindling of seizures is associated with significant changes in the concentration of kynurenic acid (KYNA) and its precursor, tryptophan (TRP). The primary finding of our study was an increase in KYNA levels and the KYNA/TRP ratio (a theoretical index of activity of the kynurenine pathway) in the amygdala and hippocampus of kindled animals. We also found decreases in the concentration of tryptophan in the hippocampus and prefrontal cortex. Changes in the concentration of KYNA and TRP in the amygdala were accompanied by a significant decrease in γ-Aminobutryic Acid (GABA) levels and an increase in the glutamate/GABA ratio. Moreover, we found a significant negative correlation between the local concentrations of KYNA and glutamate in the amygdala of kindled rats. However, there were no changes in the local concentrations of the following amino acids: glutamate, aspartate, glutamine, glycine, taurine and alanine. In conclusion, these new results suggest a modulatory influence of KYNA on the process of epileptogenesis, characterized by a negative relationship between the KYNA and glutamate systems in the amygdala.
authors.affiliation: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927, Warsaw, Poland. jszyndler@yahoo.com
authors.forename: J
authors.initials: J
authors.lastname: Szyndler
authors.forename: P
authors.initials: P
authors.lastname: Maciejak
authors.forename: D
authors.initials: D
authors.lastname: Turzyńska
authors.forename: A
authors.initials: A
authors.lastname: Sobolewska
authors.forename: J
authors.initials: J
authors.lastname: Walkowiak
authors.forename: A
authors.initials: A
authors.lastname: Płaźnik
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D004558:Electric Stimulation / Q000009:adverse effects; D004567:Electrodes, Implanted; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000378:metabolism*; D007736:Kynurenic Acid / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D000596:Amino Acids; D007736:Kynurenic Acid