title: [Quantification of acrylamide in foods by gas chromatography-mass spectrometry coupled with pro-column derivatization].
abstract: The quantification of acrylamide in foods was investigated by gas chromatography-mass spectrometry (GC-MS). In this method, (13)C3-acrylamide was used as an internal standard. The samples were homogenized, defatted with hexane and extracted with ethyl acetate for twice. The derivatization of acrylamide into 2-bromopropenamide (2-BPA) was done using potassium bromide/potassium bromate at 4 degrees C for 90 min. Selective ion monitoring mode was chosen to monitor objective chromatography. This method had a good linearity between 0.05-2.00 mg/kg (r2 = 0.9995), and the limits of detection and quantification were 3 microg/kg and 7 microg/kg, respectively. The recoveries of acrylamide were in the range of 62.7%-65.5%. In order to confirm this GC-MS method, the acrylamide in foods was also determined by high performance liquid chromatography coupled to quadrupole tandem mass spectrometry (HPLC-MS/MS), which had been proved in our laboratory. The concentrations of acrylamide in the samples quantified by GC-MS were slightly higher than those detected by HPLC-MS/MS. This method can be used to quantify acrylamide in normal foods.
authors.affiliation: College of Food Science & Nutritional Engineering, Key Laboratory of Fruits and Vegetables Processing of Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing of Ministry of Education, China Agricultural University, Beijing 100083, China.
authors.forename: Sichao
authors.initials: S
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Junhan
authors.initials: J
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*
chemical_list: D020106:Acrylamide