title: Simultaneous determination of three polyphenols in rat plasma after orally administering hawthorn leaves extract by the HPLC method.
abstract: A simple and sensitive HPLC method was developed to simultaneously determine three active compounds, vitexin-4″-O-glucoside (VG), vitexin-2″-O-rhamnoside (VR) and hyperoside (HP), in rat plasma after administering the hawthorn leaves extract (HLE). An HPLC assay with baicalin as the internal standard was carried out using a Phenomsil C₁₈ analytical column with UV detection at 332 nm. The mobile phase consisted of methanol-acetonitrile-tetrahydrofuran-1% glacial acetic acid (6 : 1.5 : 18.5 : 74, v/v/v/v). The calibration curves were linear over the range of 2.5-500, 0.2-25 and 0.25-12.5 µg mL⁻¹ for VG, VR and HP, respectively. The method was reproducible and reliable, with relative standard deviations of the intra- and inter-day precision between 1.2% and 13.2% for the analysis of the three analytes. The validated HPLC method herein described was successfully applied to the pharmacokinetic study of VG, VR and HP after oral administration of HLE to rats over the dose range of 2.5-10  mL kg⁻¹.
authors.affiliation: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, 77 Shengming 1 Road, DD Port, Dalian 116600, China.
authors.forename: Xixiang
authors.initials: X
authors.lastname: Ying
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Meng
authors.forename: Siyuan
authors.initials: S
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Du
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Tingguo
authors.initials: T
authors.lastname: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D027825:Crataegus / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000097:blood*; D051381:Rats
chemical_list: D010936:Plant Extracts; D059808:Polyphenols