title: A new way to detect the interaction of DNA and anticancer drugs based on the decreased resonance light scattering signal and its potential application.
abstract: A novel assay has been developed to detect the interaction of DNA and anticancer drugs based on the decreased resonance light scattering (RLS) technique. The proposed method can be used to study those drugs which do not produce a RLS-signal after binding to DNA. RLS was used to monitor the interaction of five anticancer drugs with DNA. The reaction between anticancer drugs and DNA took place in BR buffer solution. From the RLS assay, the sequence of five anticancer drugs activities was as follows: CTX < MTX < Pt < MMC < 5-Fu. Mammary cancer cell DNA (mcDNA) was involved to validate the RLS assay. The results showed that the sensitivities of the five anticancer drugs targeting both mcDNA and ctDNA increased in the same order. However the sensitivity of each drug to mcDNA was higher than that to ctDNA It is a significant innovation of the RLS method to detect the interaction of DNA and anticancer drugs and to obtain drug sensitivity, which provides new strategies to screen DNA targeted anticancer drugs.
authors.affiliation: Department of Chemistry, Shantou University, Shantou, 515063, China. kqlu@stu.edu.cn
authors.forename: Zhanguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Tianhe
authors.initials: T
authors.lastname: Song
authors.forename: Yurui
authors.initials: Y
authors.lastname: Peng
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.forename: Guomin
authors.initials: G
authors.lastname: Zhang
authors.forename: Sihua
authors.initials: S
authors.lastname: Qian
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000032:analysis* / Q000187:drug effects; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008027:Light; D008751:Methylene Blue / Q000737:chemistry; D015394:Molecular Structure; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D005456:Fluorescent Dyes; D008751:Methylene Blue