title: Fibrinolytic activity of kaempferol isolated from the fruits of Lagenaria siceraria (Molina) Standley.
abstract: This study was undertaken to isolate a flavonol, kaempferol, from the fruits of Lagenaria siceraria (bottle gourd) as a sole compound and to explore the fibrinolytic potential of the methanolic extract of the fruits of L. siceraria and the isolated compound using their in vitro activity. The fibrinolytic activity in terms of percentage of plasma clot liquefaction was determined by plasma clot lysis at 37°C in 24 h. The fibrinolytic activity of both substances was compared to the well-known thrombolytic agent streptokinase (30,000 IU). The percentage of fibrinolytic activity of the extract and isolated compound were found to be 54.72 ± 0.7210 and 77.37 ± 1.3010, respectively. Streptokinase was considered as the standard fibrinolytic enzyme for comparative purposes and had 91.46 ± 0.7625% fibrinolytic activity. The conclusion drawn in our study after testing the hypothesis by experimental procedures is that in vitro fibrinolytic activity on plasma clots is an inherent property of kaempferol isolated from the fruits of L. siceraria, and its comparison with streptokinase is a new aspect for further study.
authors.affiliation: College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore 452012, Madhya Pradesh, India. mithun.sgsits@gmail.com
authors.forename: M S
authors.initials: MS
authors.lastname: Rajput
authors.forename: Vineet
authors.initials: V
authors.lastname: Mathur
authors.forename: Purti
authors.initials: P
authors.lastname: Agrawal
authors.forename: H K
authors.initials: HK
authors.lastname: Chandrawanshi
authors.forename: Urmila
authors.initials: U
authors.lastname: Pilaniya
mesh_terms: D002855:Chromatography, Thin Layer; D019662:Cucurbitaceae / Q000737:chemistry*; D005343:Fibrinolytic Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005343:Fibrinolytic Agents; D044949:Kaempferols; D010936:Plant Extracts; C006552:kaempferol