title: DNA methylation profiling in nanochannels.
abstract: We report the profiling of the 5-methyl cytosine distribution within single genomic-sized DNA molecules at a gene-relevant resolution. This method linearizes and stretches DNA molecules by confinement to channels with a dimension of about 250×200 nm(2). The methylation state is detected using fluorescently labeled methyl-CpG binding domain proteins (MBD), with high signal contrast and low background. DNA barcodes consisting of methylated and non-methylated segments are generated, with both short and long concatemers demonstrating spatially resolved MBD binding. The resolution of the technique is better than 10 kbp, and single-molecule read-lengths exceeding 140 kbp have been achieved.
authors.affiliation: Department of Physics, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Shuang Fang
authors.initials: SF
authors.lastname: Lim
authors.forename: Alena
authors.initials: A
authors.lastname: Karpusenko
authors.forename: John J
authors.initials: JJ
authors.lastname: Sakon
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hook
authors.forename: Tyra A
authors.initials: TA
authors.lastname: Lamar
authors.forename: Robert
authors.initials: R
authors.lastname: Riehn