title: Immobilization of Candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support.
abstract: The overall objective of this study is to evaluate the morphological [scanning electron microscopy (SEM)], physicochemical [differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), chemical composition analysis, Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR)], and biochemical properties of Candida rugosa lipase (CRL) immobilized on a natural biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (PHBV) in aqueous solution. CRL was immobilized by physical adsorption with efficiency of 30%. Compared with free CRL enzyme, there were slight changes in immobilized CRL activity as a function of temperature (from 37°C to 45°C), but a similar optimal pH value of 7.0. Inactivation rate constants for immobilized CRL enzyme were 0.009 and 0.334 h⁻¹, and half-lives were 77 and 2 h at 40°C and 60°C, respectively. Kinetic parameters obtained for immobilized CRL include the Michaelis-Menten constant of K(m) = 213.18 mM and maximum reaction velocity of V(max) = 318.62 U/g. The operational stability of immobilized CRL was tested repeatedly, and after 12 cycles of reuse, the enzyme retained 50% activity. Based on our results, we propose that PHBV-immobilized CRL could serve as a promising biocatalyst in several industrial applications.
authors.affiliation: Universidade Tiradentes, Av. Murilo Dantas, 300, Bairro Farolândia, Aracaju, SE 49032-490, Brazil.
authors.forename: Rebeca Y
authors.initials: RY
authors.lastname: Cabrera-Padilla
authors.forename: Milena C
authors.initials: MC
authors.lastname: Lisboa
authors.forename: Alini T
authors.initials: AT
authors.lastname: Fricks
authors.forename: Elton
authors.initials: E
authors.lastname: Franceschi
authors.forename: Alvaro S
authors.initials: AS
authors.lastname: Lima
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Silva
authors.forename: Cleide M F
authors.initials: CM
authors.lastname: Soares
mesh_terms: D000327:Adsorption; D001704:Biopolymers / Q000737:chemistry; D001709:Biotechnology; D002175:Candida / Q000201:enzymology* / Q000378:metabolism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D055772:Green Chemistry Technology; D007700:Kinetics; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D000094122:Polyhydroxybutyrates
chemical_list: D001704:Biopolymers; D004800:Enzymes, Immobilized; D008049:Lipase; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates