title: Combinatorial approach for fabrication of coatings to control bacterial adhesion.
abstract: Due to the high importance of bacterial infections in medical devices there is an increasing interest in the design of anti-fouling coatings. The application of substrates with controlled chemical gradients to prevent microbial adhesion is presented. We describe here the co-polymerization of poly(ethylene glycol) dimethacrylate with a hyperbranched multimethacrylate (H30MA) using a chemical gradient generator; and the resulting films were characterized with respect to their ability to serve as coating for biomedical devices. The photo-polymerized materials present special surface properties due to the hyperbranched structure of H30MA and phase separation at specific concentrations in the PEGDM matrix. This approach affords the investigation of cell response to a large range of different chemistries on a single sample. Two bacterial strains commonly associated with surgical site infections, Escherichia coli and Pseudomonas aeruginosa, have been cultured on these substrates to study their attachment behaviour. These gradient-coated samples demonstrate less bacterial adhesion at higher concentrations of H30MA, and the adhesion is substantially affected by the extent of surface phase segregation.
authors: a Institute de Ciencia y Tecnología de Polímeros, CSIC , Juan de la Cierva 3 , 28006 , Madrid , Spain.
authors: S
authors: S
authors: Pedron
authors: C
authors: C
authors: Peinado
authors: F
authors: F
authors: Catalina
authors: P
authors: P
authors: Bosch
authors: K S
authors: KS
authors: Anseth
authors: C
authors: C
authors: Abrusci
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D057219:Biofouling / Q000517:prevention & control*; D004926:Escherichia coli / Q000502:physiology; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D058105:Polymerization; D011550:Pseudomonas aeruginosa / Q000502:physiology; D013499:Surface Properties
chemical_list: D008689:Methacrylates; C421283:poly(ethylene glycol)-dimethacrylate; D011092:Polyethylene Glycols