title: A quantitative immunopolymerase chain reaction method for detection of vegetative insecticidal protein in genetically modified crops.
abstract: Vegetative insecticidal protein (Vip) is being employed for transgenic expression in selected crops such as cotton, brinjal, and corn. For regulatory compliance, there is a need for a sensitive and reliable detection method, which can distinguish between approved and nonapproved genetically modified (GM) events and quantify GM contents as well. A quantitative immunopolymerase chain reaction (IPCR) method has been developed for the detection and quantification of Vip protein in GM crops. The developed assay displayed a detection limit of 1 ng/mL (1 ppb) and linear quantification range between 10 and 1000 ng/mL of Vip-S protein. The sensitivity of the assay was found to be 10 times higher than an analogous enzyme-linked immunosorbent assay for Vip-S protein. The results suggest that IPCR has the potential to become a standard method to quantify GM proteins.
authors: Department of Botany (Centre of Advanced Study), Banaras Hindu University , Varanasi-221 005, India. rajeshsbhu@yahoo.com
authors: Rajesh
authors: R
authors: Kumar
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay / Q000379:methods*; D007306:Insecticides; D030821:Plants, Genetically Modified / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D007306:Insecticides; D011994:Recombinant Proteins; C447139:Vip3A protein, Bacillus thuringiensis