title: Cell-free ligand-binding assays for integrin LFA-1.
abstract: Integrin LFA-1 plays an important role in leukocyte trafficking to inflammatory sites as well as the interaction of T cells with antigen-presenting cells. Inhibition of the LFA-1:ICAM-1 interactions is a promising therapeutic approach for alleviating inflammation and autoimmune diseases. This chapter describes cell-free assays to study the LFA-1:ICAM-1 interactions. These assays may be used for screening novel LFA-1 antagonists.
authors: Department of Anesthesiology, Perioperative and Pain Medicine, Children's Hospital Boston, Boston, MA, USA. yuki@idi.harvard.edu
authors: Koichi
authors: K
authors: Yuki
mesh_terms: D002474:Cell-Free System; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1