title: Continuous CD8⁺ T-cell priming by dendritic cell cross-presentation of persistent antigen following adeno-associated virus-mediated gene delivery.
abstract: Recombinant adeno-associated virus (rAAV) vectors establish persistent transgene expression in the skeletal muscle of mice. How dendritic cells acquire encoded antigens for CD8(+) T-cell priming is unknown. Here we document CD8(+) T-cell priming after lethal irradiation and bone marrow reconstitution of mice treated with an AAV vector several weeks earlier. Temporal separation of vector delivery and successful class I antigen presentation indicated that T-cell priming does not necessarily require antigen synthesis in AAV-transduced dendritic cells. An apparent cross-presentation of antigen acquired from muscle suggests that strategies to limit transgene expression in dendritic cells will not prevent unwanted CD8(+) T-cell responses.
authors: Center for Vaccines and Immunity, The Research Institute at Nationwide Children's Hospital, 700 Children's Drive, Columbus, OH 43205, USA.
authors: Dan
authors: D
authors: Xu
authors: Christopher M
authors: CM
authors: Walker
mesh_terms: D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D045142:Cross-Priming; D003713:Dendritic Cells / Q000276:immunology*; D000229:Dependovirus / Q000235:genetics*; D005326:Fetal Proteins; D005796:Genes; D005822:Genetic Vectors; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D020825:T-Box Domain Proteins; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000235:genetics / Q000276:immunology; D000098908:Brachyury Protein
chemical_list: D000941:Antigens; D005326:Fetal Proteins; D020825:T-Box Domain Proteins; D001616:beta-Galactosidase; D000098908:Brachyury Protein