title: Capillary electrophoresis of free fatty acids by indirect ultraviolet detection: application to the classification of vegetable oils according to their botanical origin.
abstract: A method for the determination of fatty acids in vegetable oils by capillary electrophoresis with indirect UV-vis detection has been developed. The separation of fatty acids was optimized in terms of Brij surfactant nature and concentration and organic modifier (2-propanol) percentage. The optimal background electrolyte consisted of 10 mM p-hydroxybenzoate, 5 mM Tris at pH 8.8, 80 mM Brij 98, 40% acetonitrile, and 10% 2-propanol. Under these conditions, vegetable oils from five botanical origins (avocado, corn, extra virgin olive, hazelnut, and soybean) were analyzed and the fatty acid contents established. Linear discriminant analysis (LDA) models were constructed using fatty acid peak areas as predictors. An excellent resolution among all category pairs was obtained, and all samples were correctly classified with assignment probabilities of >95%.
authors: Departamento de Química Analítica, Universidad de Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
authors: María
authors: M
authors: Vergara-Barberán
authors: Aarón
authors: A
authors: Escrig-Doménech
authors: María Jesús
authors: MJ
authors: Lerma-García
authors: Ernesto Francisco
authors: EF
authors: Simó-Alfonso
authors: José Manuel
authors: JM
authors: Herrero-Martínez
mesh_terms: D019840:2-Propanol; D019075:Electrophoresis, Capillary / Q000379:methods*; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry* / Q000145:classification*; D011092:Polyethylene Glycols
chemical_list: D005230:Fatty Acids, Nonesterified; D010938:Plant Oils; D011092:Polyethylene Glycols; C033084:polyethylene glycol oleyl ether; D019840:2-Propanol