title: Bacterial killing by several root filling materials and methods in an ex vivo infected root canal model.
abstract: OBJECTIVE
  To evaluate the ability of two root canal sealers (Epoxy resin-based AH Plus or polydimethylsiloxane-based GuttaFlow) and five root filling techniques (continuous wave of condensation, Thermafil, lateral condensation, matched taper single gutta-percha point, laterally condensed-matched taper gutta-percha point) to kill bacteria in experimentally infected dentinal tubules.
  
  
  METHODS
  An infected dentine block model was used. One hundred and twenty extracted, single-rooted human teeth were randomly divided into 10 test (n = 10) and 2 control (n = 10) groups. The roots, except negative controls, were infected with Enterococcus faecalis for 21 days. The root canals were then filled using the test materials and methods. Positive controls were not filled. Sterile roots were used as negative controls. Dentine powder was obtained from all root canals using gates glidden drills using a standard method. The dentine powder was diluted and inoculated into bacterial growth media. Total colony-forming units (CFU) were calculated for each sample. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U test.
  
  
  RESULTS
  The epoxy resin-based sealer was effective in killing E. faecalis except when using Thermafil (P < 0.05), but the polydimethylsiloxane-based sealer was not effective in killing this microorganism except in the continuous wave group (P < 0.05).
  
  
  CONCLUSIONS
  In the test model, AH Plus killed bacteria in infected dentine more effectively than GuttaFlow. The filling method was less important than the sealer material.
authors: Department of Endodontics, Faculty of Dentistry, Mustafa Kemal University, Hatay, Turkey. eozcan58@hotmail.com
authors: E
authors: E
authors: Özcan
authors: A U
authors: AU
authors: Eldeniz
authors: H
authors: H
authors: Arı
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D058491:Bacterial Load / Q000187:drug effects; D001431:Bacteriological Techniques; D003786:Dental Pulp Cavity / Q000382:microbiology* / Q000648:ultrastructure; D003804:Dentin / Q000382:microbiology / Q000648:ultrastructure; D004129:Dimethylpolysiloxanes / Q000627:therapeutic use; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects*; D004853:Epoxy Resins / Q000627:therapeutic use; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy*; D006171:Gutta-Percha / Q000627:therapeutic use; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D012387:Root Canal Filling Materials / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012389:Root Canal Obturation / Q000379:methods*; D018915:Root Canal Preparation / Q000295:instrumentation; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; D004338:Drug Combinations; D004853:Epoxy Resins; C515718:GuttaFlow; D012387:Root Canal Filling Materials; D012388:Root Canal Irrigants; C534916:epoxy resin-based root canal sealer; D006171:Gutta-Percha; D012973:Sodium Hypochlorite