title: Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
abstract: The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype. Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.
authors: Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Suedufer 10, 17493 Greifswald, Riems Island, Germany. Bernd.Hoffmann@fli.bund.de
authors: Bernd
authors: B
authors: Hoffmann
authors: Sandra
authors: S
authors: Blome
authors: Paolo
authors: P
authors: Bonilauri
authors: Jovita
authors: J
authors: Fernández-Piñero
authors: Irene
authors: I
authors: Greiser-Wilke
authors: Andy
authors: A
authors: Haegeman
authors: Mats
authors: M
authors: Isaksson
authors: Frank
authors: F
authors: Koenen
authors: Neil
authors: N
authors: LeBlanc
authors: Immanuel
authors: I
authors: Leifer
authors: Marie-Frederique
authors: MF
authors: Le Potier
authors: Willie
authors: W
authors: Loeffen
authors: Thomas Bruun
authors: TB
authors: Rasmussen
authors: Tomasz
authors: T
authors: Stadejek
authors: Karl
authors: K
authors: Ståhl
authors: Marylène
authors: M
authors: Tignon
authors: Ase
authors: A
authors: Uttenthal
authors: Wim
authors: W
authors: van der Poel
authors: Martin
authors: M
authors: Beer
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000453:epidemiology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D005060:Europe; D005838:Genotype; D007753:Laboratories; D015588:Observer Variation; D012367:RNA, Viral / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D012367:RNA, Viral