title: Validation of tumor-associated macrophage ferritin light chain as a prognostic biomarker in node-negative breast cancer tumors: A multicentric 2004 national PHRC study.
abstract: Novel prognostic biomarkers are imperatively needed to help direct treatment decisions by typing subgroups of node-negative breast cancer patients. Large screening of different biological compartments, such as the proteome, by means of high throughput techniques may greatly help scientists to find such markers. The present retrospective multicentric study included 268 node-negative breast cancer patients. We used a proteomic approach of SELDI-TOF-MS screening to identify differentially expressed cytosolic proteins with prognostic impact. The screening cohort was composed of 198 patients. Seventy supplementary patients were included for validation. Immunohistochemistry (IHC) and immunoassay (IA) were run to confirm the prognostic role of the marker identified by SELDI-TOF-MS screening. IHC was also used to explore links between selected marker and epithelial-mesenchymal transition (EMT)-like, proliferation and macrophage markers. Ferritin light chain (FTL) was identified as an independent prognostic marker (HR = 1.30-95% CI: 1.10-1.50, p = 0.001). Validation step by means of IHC and IA confirmed the prognostic value of FTL level. CD68 IHC showed that FTL was stored in tumor-associated macrophages (TAM), which exhibit an M2-like phenotype. We report here, first, the validation of FTL as a breast tumor prognostic biomarker in node-negative patients, and second, the fact that FTL is stored in TAM.
authors: Département de Biologie Oncologique, Institut de Cancérologie de l'Ouest - René Gauducheau, Bd J Monod, Nantes - Saint Herblain Cedex, France. pascal.jezequel@ico.unicancer.fr
authors: Pascal
authors: P
authors: Jézéquel
authors: Loïc
authors: L
authors: Campion
authors: Frédérique
authors: F
authors: Spyratos
authors: Delphine
authors: D
authors: Loussouarn
authors: Mario
authors: M
authors: Campone
authors: Catherine
authors: C
authors: Guérin-Charbonnel
authors: Marie-Pierre
authors: MP
authors: Joalland
authors: Jean
authors: J
authors: André
authors: Françoise
authors: F
authors: Descotes
authors: Catherine
authors: C
authors: Grenot
authors: Pascal
authors: P
authors: Roy
authors: Antoine
authors: A
authors: Carlioz
authors: Pierre-Marie
authors: PM
authors: Martin
authors: Agnès
authors: A
authors: Chassevent
authors: Marie-Lise
authors: ML
authors: Jourdan
authors: Gabriel
authors: G
authors: Ricolleau
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001052:Apoferritins / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000175:diagnosis* / Q000473:pathology; D049109:Cell Proliferation; D015331:Cohort Studies; D003600:Cytosol; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D005260:Female; D006801:Humans; D008264:Macrophages / Q000737:chemistry*; D008875:Middle Aged; D011379:Prognosis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001052:Apoferritins; D014408:Biomarkers, Tumor; C067980:CD68 antigen, human; D000097382:CD68 Molecule