title: Purification and physicochemical properties of different polysaccharide fractions from the water extract of Boschniakia rossica and their effect on macrophages activation.
abstract: Today more and more attentions had been attracted by many nutritionists and pharmacologists on polysaccharides from natural plants or animals due to their significant biological activities. In this research three polysaccharides (BRR-W1, BRR-WA1 and BRR-WA2) were isolated and purified from the water extract of Boschniakia rossica by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Chemical and physical characteristics of three polysaccharides were investigated by a combination of chemical and instrumental analysis methods. The assays of their effect on macrophages activation were also investigated in vitro, including phagocytosis of macrophages, detections for NO production and TNF-α secretion. The results indicated that the effect of polysaccharides on macrophages activation was influenced by their respective physicochemical properties.
authors: School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China.
authors: Yang
authors: Y
authors: Liu
authors: Yu
authors: Y
authors: Sheng
authors: Guangxin
authors: G
authors: Yuan
authors: Yan
authors: Y
authors: Wang
authors: Huifang
authors: H
authors: Wei
authors: Ming
authors: M
authors: Guan
authors: Jin
authors: J
authors: Pei
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D031670:Orobanchaceae / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology; D012685:Sepharose; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D012685:Sepharose