title: Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no. 7.
abstract: The term 'sake yeast' is generally used to indicate the Saccharomyces cerevisiae strains that possess characteristics distinct from others including the laboratory strain S288C and are well suited for sake brewery. Here, we report the draft whole-genome shotgun sequence of a commonly used diploid sake yeast strain, Kyokai no. 7 (K7). The assembled sequence of K7 was nearly identical to that of the S288C, except for several subtelomeric polymorphisms and two large inversions in K7. A survey of heterozygous bases between the homologous chromosomes revealed the presence of mosaic-like uneven distribution of heterozygosity in K7. The distribution patterns appeared to have resulted from repeated losses of heterozygosity in the ancestral lineage of K7. Analysis of genes revealed the presence of both K7-acquired and K7-lost genes, in addition to numerous others with segmentations and terminal discrepancies in comparison with those of S288C. The distribution of Ty element also largely differed in the two strains. Interestingly, two regions in chromosomes I and VII of S288C have apparently been replaced by Ty elements in K7. Sequence comparisons suggest that these gene conversions were caused by cDNA-mediated recombination of Ty elements. The present study advances our understanding of the functional and evolutionary genomics of the sake yeast.
authors: National Research Institute of Brewing, Higashi-hiroshima, Japan.
authors: Takeshi
authors: T
authors: Akao
authors: Isao
authors: I
authors: Yashiro
authors: Akira
authors: A
authors: Hosoyama
authors: Hiroshi
authors: H
authors: Kitagaki
authors: Hiroshi
authors: H
authors: Horikawa
authors: Daisuke
authors: D
authors: Watanabe
authors: Rinji
authors: R
authors: Akada
authors: Yoshinori
authors: Y
authors: Ando
authors: Satoshi
authors: S
authors: Harashima
authors: Toyohisa
authors: T
authors: Inoue
authors: Yoshiharu
authors: Y
authors: Inoue
authors: Susumu
authors: S
authors: Kajiwara
authors: Katsuhiko
authors: K
authors: Kitamoto
authors: Noriyuki
authors: N
authors: Kitamoto
authors: Osamu
authors: O
authors: Kobayashi
authors: Satoru
authors: S
authors: Kuhara
authors: Takashi
authors: T
authors: Masubuchi
authors: Haruhiko
authors: H
authors: Mizoguchi
authors: Yoshihiro
authors: Y
authors: Nakao
authors: Atsumi
authors: A
authors: Nakazato
authors: Masahiro
authors: M
authors: Namise
authors: Takahiro
authors: T
authors: Oba
authors: Tomoo
authors: T
authors: Ogata
authors: Akinori
authors: A
authors: Ohta
authors: Masahide
authors: M
authors: Sato
authors: Seiji
authors: S
authors: Shibasaki
authors: Yoshifumi
authors: Y
authors: Takatsume
authors: Shota
authors: S
authors: Tanimoto
authors: Hirokazu
authors: H
authors: Tsuboi
authors: Akira
authors: A
authors: Nishimura
authors: Koji
authors: K
authors: Yoda
authors: Takeaki
authors: T
authors: Ishikawa
authors: Kazuhiro
authors: K
authors: Iwashita
authors: Nobuyuki
authors: N
authors: Fujita
authors: Hitoshi
authors: H
authors: Shimoi
mesh_terms: D007446:Chromosome Inversion; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA