title: Lentiviral gene transfer method to study integrin function in T lymphocytes.
abstract: Integrins play critical roles in adhesion and migration of T cells during an immune response and inflammation. It is of great importance to understand the molecular pathways that regulate integrin function in T cells. Lentiviral vector-based gene transfer method has emerged in the past decade as an efficient means of transferring genes into both resting and activated hard-to-transfect cells, including T cells to knockdown gene expression. Therefore, this technology could be utilized effectively to study different aspects of integrin function or even to perform genome-wide RNAi screens to look globally for regulators of integrin function in T cells. In this chapter, we provide the simplest protocol to infect activated CD4(+) human T cells with high efficiency.
authors: Immune Disease Institute, Program in Cellular and Molecular Medicine, Children's Hospital Boston, Boston, MA, USA. banerjee@idi.harvard.edu
authors: Daliya
authors: D
authors: Banerjee
authors: Motomu
authors: M
authors: Shimaoka
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D016086:Lentivirus / Q000235:genetics*; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000378:metabolism*; D014161:Transduction, Genetic
chemical_list: D016023:Integrins