title: Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region.
abstract: We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil.
authors: Laboratório de Bacteriologia Clínica, Fundação Alfredo da Matta, Universidade Estadual do Amazonas, Manaus, AM, Brasil, 69065-130. wianfe@yahoo.com.br
authors: William Antunes
authors: WA
authors: Ferreira
authors: Cristina Motta
authors: CM
authors: Ferreira
authors: Felipe Gomes
authors: FG
authors: Naveca
authors: Nayanne Cristina Oliveira da Silva
authors: NC
authors: Almeida
authors: Waldemara de Souza
authors: Wde S
authors: Vasconcelos
authors: Jairo de Souza
authors: Jde S
authors: Gomes
authors: Maria de Fátima Pinto da
authors: Mde F
authors: Silva
authors: Maria das Graças Costa
authors: Md
authors: Alecrim
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001938:Brazil; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005838:Genotype; D006069:Gonorrhea / Q000175:diagnosis / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D009344:Neisseria gonorrhoeae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D015242:Ofloxacin / Q000494:pharmacology*; D010641:Phenotype
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D015242:Ofloxacin