title: Experimental study on phase-contrast imaging with synchrotron hard X-ray for repairing osteonecrosis of the femoral head.
abstract: Synchrotron radiation light is 1 of 4 artificial light sources, the others being electric light, X-ray, and laser. Phase-contrast imaging with hard X-ray has achieved wide application in many scientific fields, such as biomedicine and material science. This article compares the effectiveness of nanohydroxyapatite/collagen (nHAC) and autologous mesenchymal stem cell for the repair of defects in a rabbit model with osteonecrosis of the femoral head under the monitoring of phase-contrast imaging with synchrotron hard X-ray. We established models of bilateral osteonecrosis of the femoral head defect using New Zealand rabbits and divided them into 3 groups. Imaging techniques such as phase-contrast imaging and diffraction enhanced imaging with synchrotron hard X-ray were applied to assess the degradation and repair process of nHAC and mesenchymal stem cell at 4, 8, and 12 weeks postoperatively. We found phase-contrast imaging with synchrotron hard X-ray displayed the reparative process of the bone defect, degradation of nHAC, and osteocyte substitution. There were significant differences in the repair of the bone defect and osteogenesis in groups B and C compared with group A (control). Osteogenesis was more significant in group C. We provided experimental data for the development and application of synchrotron hard X-ray imaging techniques and concluded that phase-contrast microimaging with synchrotron hard X-ray displays the reparative process of bone tissue at a micro-level and plays an important role in the development of tissue engineering.
authors: Center for Osteonecrosis and Joint Preserving and Reconstruction, Department of Orthopedics, China-Japan Friendship Hospital, Beijing, China.
authors: Wei
authors: W
authors: Sun
authors: Zi-rong
authors: ZR
authors: Li
authors: Yu-Run
authors: YR
authors: Yang
authors: Zhen-cai
authors: ZC
authors: Shi
authors: Bailiang
authors: B
authors: Wang
authors: Bingli
authors: B
authors: Liu
authors: Shaohui
authors: S
authors: Shi
mesh_terms: D000818:Animals; D003094:Collagen / Q000494:pharmacology; D004195:Disease Models, Animal; D017886:Durapatite / Q000494:pharmacology; D005260:Female; D005271:Femur Head Necrosis / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D011859:Radiography / Q000295:instrumentation / Q000379:methods*; D017356:Synchrotrons; D023822:Tissue Engineering
chemical_list: C509317:nano-hydroxyapatite-collagen; D003094:Collagen; D017886:Durapatite