title: IL-10 neutralization promotes parasite clearance in splenic aspirate cells from patients with visceral leishmaniasis.
abstract: The mechanisms underlying the failure to contain the growth of Leishmania parasites in human visceral leishmaniasis (VL) are not understood. L donovani amastigotes were quantified in cultured splenic aspirate cells to assess the function of IL-10 in lesional tissue ex vivo. In 67 patients with active VL, IL-10 neutralization promoted parasite killing in 73% and complete clearance in 30%, while 18% had more parasites and 9% did not change. The splenic cells secreted increased levels of both tumor necrosis factor α (TNFα) and interferon γ (IFNγ) under IL-10-neutralizing conditions. These findings provide direct support for targeting IL-10 as an approach to therapy in human VL.
authors: Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
authors: Shalini
authors: S
authors: Gautam
authors: Rajiv
authors: R
authors: Kumar
authors: Radheshyam
authors: R
authors: Maurya
authors: Susanne
authors: S
authors: Nylén
authors: Nasim
authors: N
authors: Ansari
authors: Madhukar
authors: M
authors: Rai
authors: Shyam
authors: S
authors: Sundar
authors: David
authors: D
authors: Sacks
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D007893:Leishmania donovani / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology*; D008264:Macrophages / Q000378:metabolism / Q000469:parasitology; D008297:Male; D013154:Spleen / Q000378:metabolism / Q000469:parasitology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D055815:Young Adult
chemical_list: D000911:Antibodies, Monoclonal; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma