title: The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells.
abstract: Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibility of using the AM after different preservation methods to serve as substrates for endothelial cell expansion ex vivo. The effects of cryopreservation and lyophilization were evaluated on mechanical and histological characteristics of the AM, and the results were compared with the fresh AM. The ECM components of the basement membrane were well conserved in all groups. Although lyophilization resulted in more histological changes and lower level of physical variables including thickness, F(max), elongation at break and suture retention than the fresh and cryopreserved AM, endothelial cells grown on the lyophilized AM were better attached to the basement membrane. Cytotoxicity assay by MTT showed that the lyophilized AM is a compatible substrate for endothelial cells cultivation. The findings of this study suggest that the lyophilized AM is a suitable matrix for cultivation of endothelial cells due to this fact that lyophilization led to exposure of basement membrane of the AM.
authors: Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. niknejad@sbmu.ac.ir
authors: Hassan
authors: H
authors: Niknejad
authors: Tina
authors: T
authors: Deihim
authors: Mehran
authors: M
authors: Solati-Hashjin
authors: Habibollah
authors: H
authors: Peirovi
mesh_terms: D000650:Amnion / Q000033:anatomy & histology* / Q000378:metabolism; D000818:Animals; D001485:Basement Membrane / Q000033:anatomy & histology* / Q000378:metabolism; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D002585:Cesarean Section; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis; D015925:Cryopreservation / Q000379:methods*; D042783:Endothelial Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D005353:Fibronectins / Q000032:analysis / Q000096:biosynthesis; D005612:Freeze Drying / Q000379:methods*; D019812:Heparan Sulfate Proteoglycans / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007797:Laminin / Q000032:analysis / Q000096:biosynthesis; D008855:Microscopy, Electron, Scanning; D010920:Placenta / Q000033:anatomy & histology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D005353:Fibronectins; D019812:Heparan Sulfate Proteoglycans; D007797:Laminin; C071927:perlecan; D003094:Collagen