title: Inhibitory effect of pomegranate on intestinal sodium dependent glucose uptake.
abstract: Intestinal glucose uptake is mainly performed by its specific transporters, SGLT1 and GLUTs expressed in the intestinal epithelial cells. By using Caco-2 cells and 2-NBDG, we observed that intestinal glucose uptake was markedly inhibited by pomegranate (Punica granatum L, PG) among 200 screened edible Korean plants. The effects of the PG extract on Na(+)-dependent glucose uptake were further evaluated using brush border membrane vesicles (BBMV) obtained from the mouse small intestine. PG inhibited Na(+)-dependent glucose uptake with the IC(50) value of 424 μg/ml. The SGLT1 protein expression was dose dependently down regulated with PG treatment in Caco-2 cells. We next assessed the antihyperglycemic effect of PG in streptozotocin (STZ)-induced diabetic mice. Administration of PG (800 mg/kg) to STZ mice for four weeks improved postprandial glucose regulation. Furthermore, elevated Na(+)-dependent glucose uptake by BBMV isolated from STZ mice was normalized by PG treratment. These results suggest that PG could play a role in controlling the dietary glucose absorption at the intestinal tract by decreasing SGLT1 expression, and may contribute to blood glucose homeostasis in the diabetic condition.
authors: Department of Food and Biotechnology, Hanseo University, Seosan 356-706, Republic of Korea.
authors: Hye Kyung
authors: HK
authors: Kim
authors: Soon-Sun
authors: SS
authors: Baek
authors: Hong-Yon
authors: HY
authors: Cho
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects*; D018938:Caco-2 Cells; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006943:Hyperglycemia / Q000188:drug therapy* / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012964:Sodium / Q000378:metabolism*
chemical_list: D007004:Hypoglycemic Agents; D012964:Sodium; D005947:Glucose