title: Knockdown of aquaporin 3 is involved in intestinal barrier integrity impairment.
abstract: AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 translocation, with the reduction of TEER and increasing LY permeability. Western blots revealed that expression of Claudin-1 and Occludin were significantly decreased in the AQP3 knockdown group, demonstrating that this treatment enhances paracellular permeability via an opening of the tight junction complex. These data not only describe the correlation between transcellular and paracellular pathways in human intestines, but also show that targeted knockdown of AQP3 might impair the intestinal barrier integrity.
authors: Division of Gastrointestinal Surgery, Department of General Surgery, First Affiliated Hospital, Nanjing Medical University, Nanjing, China.
authors: Wenjie
authors: W
authors: Zhang
authors: Yong
authors: Y
authors: Xu
authors: Zheng
authors: Z
authors: Chen
authors: Zekuan
authors: Z
authors: Xu
authors: Hao
authors: H
authors: Xu
mesh_terms: D051400:Aquaporin 3 / Q000235:genetics* / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D002463:Cell Membrane Permeability / Q000502:physiology*; D062445:Claudin-1; D017097:Electric Impedance; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000382:microbiology / Q000502:physiology* / Q000503:physiopathology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D062793:Occludin; D034622:RNA Interference; D019108:Tight Junctions / Q000378:metabolism
chemical_list: C568108:CLDN1 protein, human; D062445:Claudin-1; D008565:Membrane Proteins; C568985:OCLN protein, human; D062793:Occludin; D051400:Aquaporin 3