title: Circadian expression of steroidogenic cytochromes P450 in the mouse adrenal gland--involvement of cAMP-responsive element modulator in epigenetic regulation of Cyp17a1.
abstract: The cytochrome P450 (CYP) genes Cyp51, Cyp11a1, Cyp17a1, Cyb11b1, Cyp11b2 and Cyp21a1 are involved in the adrenal production of corticosteroids, whose circulating levels are circadian. cAMP signaling plays an important role in adrenal steroidogenesis. By using cAMP responsive element modulator (Crem) knockout mice, we show that CREM isoforms contribute to circadian expression of steroidogenic CYPs in the mouse adrenal gland. Most striking was the CREM-dependent hypomethylation of the Cyp17a1 promoter at zeitgeber time 12, which resulted in higher Cyp17a1 mRNA and protein expression in the knockout adrenal glands. The data indicate that products of the Crem gene control the epigenetic repression of Cyp17 in mouse adrenal glands.
authors: Center for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors: Rok
authors: R
authors: Košir
authors: Ursula Prosenc
authors: UP
authors: Zmrzljak
authors: Tanja
authors: T
authors: Bele
authors: Jure
authors: J
authors: Acimovic
authors: Martina
authors: M
authors: Perse
authors: Gregor
authors: G
authors: Majdic
authors: Cornelia
authors: C
authors: Prehn
authors: Jerzy
authors: J
authors: Adamski
authors: Damjana
authors: D
authors: Rozman
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm; D003345:Corticosterone / Q000097:blood; D051786:Cyclic AMP Response Element Modulator / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D044127:Epigenesis, Genetic / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic; D013254:Steroid 17-alpha-Hydroxylase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D051786:Cyclic AMP Response Element Modulator; D013254:Steroid 17-alpha-Hydroxylase; D003345:Corticosterone