title: Purification and characterization of a magnesium ion requiring N-acetyl-D-glucosamine specific lectin from seeds of Quercus ilex L.
abstract: A new magnesium ion requiring N-acetyl-D-glucosamine specific lectin QIL was purified to electrophoretic homogeneity from seeds of Quercus ilex L. through successive steps of (i) lectin extraction, (ii) ammonium sulphate (30-50%) fractionation, (iii) diethylaminoethyl (DEAE)-cellulose chromatography, (iv) carboxymethyl (CM)-cellulose chromatography, and (v) Sephadex G-75 chromatography. The lectin, having specific activity of 25,600 hemagglutination units (HAU)/mg of protein, was found to be a monomeric protein with a native molecular weight of 13.2 kDa. N-Acetyl-D-glucosamine was found to exhibit most potent inhibitory action on the lectin activity among all the sugars tested. The lectin was also found to exhibit specificity for human blood groups A, B, and AB. It was converted to the corresponding apo-lectin by ethylenediaminetetraacetic acid (EDTA) treatment followed by buffer dialysis. The apo-lectin exhibited a specific and characteristic requirement for magnesium ions for the expression of its activity.
authors: Laboratory of Protein Biochemistry, Biochemistry Department, Manipur University, India. kdsanjenbam@yahoo.com
authors: Sanjenbam Kunjeshwori
authors: SK
authors: Devi
authors: Senjam Sunil
authors: SS
authors: Singh
authors: Sorokhaibam Jibankumar
authors: SJ
authors: Singh
authors: Huidrom
authors: H
authors: Rully
authors: Laishram Rupachandra
authors: LR
authors: Singh
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004492:Edetic Acid; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006384:Hemagglutination / Q000187:drug effects; D006385:Hemagglutination Inhibition Tests; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D007983:Lewis Blood Group Antigens / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D029963:Quercus / Q000737:chemistry*; D011817:Rabbits; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D037102:Lectins; D007983:Lewis Blood Group Antigens; D004492:Edetic Acid; D008274:Magnesium; D000117:Acetylglucosamine