title: Zinc-doped dentin adhesive for collagen protection at the hybrid layer.
abstract: The aim of the study was to ascertain whether the addition of zinc to adhesives may decrease metalloproteinase-mediated collagen degradation without affecting bonding efficacy. Human dentin beams were treated with phosphoric acid, with Clearfil SE Bond Primer or with Clearfil SE Bond Primer plus ZnCl(2) (2 wt%). Acid-etched dentin was infiltrated with Single Bond, Single Bond plus ZnCl(2) (2 wt%), or Single Bond plus ZnO nanoparticles (10 wt%), and Clearfil SE Bond-primed dentin was infiltrated with Clearfil SE Bonding resin, Clearfil SE-Bonding resin with ZnCl(2) (2 wt%), or Clearfil SE-Bonding resin with ZnO nanoparticles (10 wt%). The C-terminal telopeptide concentrations were determined 24 h, and 1 and 4 wk after treatment. Microtensile bond strength to dentin was determined for the tested adhesives. Matrix metalloproteinases-mediated collagen degradation occurred in acid-etched and SE-primed dentin. Resin infiltration decreased collagen degradation. Lower collagen degradation was found for SE Bond than for Single Bond. Zinc-doped Single Bond resin always reduced collagen degradation, the ZnO particles being more effective than ZnCl(2) . Zinc-doped SE Bond reduced the liberation of C-terminal telopeptide only at 24 h. Bond strength to dentin was not decreased when Zn-doped resins were employed, except when ZnCl(2) was added to SE Primer. Zinc-doped resin reduced collagen degradation in Single Bond hybrid layers, but did not affect bond strength. The addition of zinc to SE Bond had no beneficial effects.
authors: Department of Dental Materials, School of Dentistry, University of Granada, Granada Institute of Polymers, CSIC, Madrid, Spain.
authors: Raquel
authors: R
authors: Osorio
authors: Monica
authors: M
authors: Yamauti
authors: Estrella
authors: E
authors: Osorio
authors: Julio S
authors: JS
authors: Román
authors: Manuel
authors: M
authors: Toledano
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D003094:Collagen / Q000648:ultrastructure*; D024042:Collagen Type I / Q000032:analysis; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D010455:Peptides / Q000032:analysis; D010756:Phosphoric Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013314:Stress, Mechanical; D013718:Tensile Strength; D013997:Time Factors; D015032:Zinc / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D002712:Chlorides; C438313:Clearfil SE Bond; D024042:Collagen Type I; D017220:Dentin-Bonding Agents; D061965:Matrix Metalloproteinase Inhibitors; D010455:Peptides; D010756:Phosphoric Acids; D019279:Resin Cements; C511238:Single Bond Plus; D017967:Zinc Compounds; C052929:collagen type I trimeric cross-linked peptide; D017438:Bisphenol A-Glycidyl Methacrylate; C016837:zinc chloride; D003094:Collagen; C030242:phosphoric acid; D015032:Zinc; D015034:Zinc Oxide