title: Cell labeling approaches for fluorescence-based in vivo flow cytometry.
abstract: We provide an overview of the methods used to label circulating cells for fluorescence detection by in vivo flow cytometry. These methods are useful for cell tracking in small animals without the need to draw blood samples and are particularly useful for the detection of circulating cancer cells and quantification of circulating immune cells.
authors: Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors: Costas M
authors: CM
authors: Pitsillides
authors: Judith M
authors: JM
authors: Runnels
authors: Joel A
authors: JA
authors: Spencer
authors: Liang
authors: L
authors: Zhi
authors: Mei X
authors: MX
authors: Wu
authors: Charles P
authors: CP
authors: Lin
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000378:metabolism; D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D051379:Mice; D057054:Molecular Imaging / Q000379:methods; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D061088:Photoacoustic Techniques / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins