title: A dual-chamber model of the female genital tract to evaluate epithelial toxicity of candidate anti-HIV microbicides.
abstract: Heterosexual transmission of human immunodeficiency virus (HIV) is the predominant mode of infection worldwide. The early steps of transepithelial infection are crucial, but how exactly infection is established in the female genital tract (FGT) is still under debate. Using epithelial cells originating from the FGT and primary cells as subepithelial HIV target cells, an in vitro dual-chamber model of the FGT was developed. Here we describe how this in vitro model can be used to assess the cellular toxicity and anti-HIV activity of antiretrovirals and formulations thereof that are intended to be used as microbicides.
authors: Virology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium.
authors: Kevin K
authors: KK
authors: Ariën
authors: Guido
authors: G
authors: Vanham
authors: Youssef
authors: Y
authors: Gali
mesh_terms: D019380:Anti-HIV Agents / Q000633:toxicity*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000821:virology; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival; D018920:Coculture Techniques / Q000379:methods; D003713:Dendritic Cells / Q000187:drug effects / Q000821:virology; D004847:Epithelial Cells / Q000187:drug effects* / Q000821:virology; D005260:Female; D005836:Genitalia, Female / Q000187:drug effects*; D015658:HIV Infections / Q000517:prevention & control / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000187:drug effects / Q000254:growth & development; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D014776:Virus Cultivation / Q000379:methods
chemical_list: D019380:Anti-HIV Agents