title: Interaction of Arabidopsis DET1 with CCA1 and LHY in mediating transcriptional repression in the plant circadian clock.
abstract: The COP10-DET1-DDB1 (CDD) complex is an evolutionarily conserved protein complex discovered for its role in the repression of photomorphogenesis in Arabidopsis. It is important in many cellular and developmental processes in both plants and animals, but its molecular mode of action remains poorly understood. Here, we show that the CDD component DET1 possesses transcriptional repression activity and physically interacts with two closely related MYB transcription factors, CCA1 and LHY, which are core components of the plant circadian clock. DET1 associates with the promoter of CCA1/LHY target genes, such as TOC1, in a CCA1/LHY-dependent manner and is required for their repression, suggesting a recruitment of DET1 by the central oscillator components to regulate the clock. Our results reveal DET1 as a core transcriptional repression factor important for clock progression. Overall, the CDD complex may function as a transcriptional corepressor in diverse processes through direct interaction with distinct transcription factors.
authors: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
authors: On Sun
authors: OS
authors: Lau
authors: Xi
authors: X
authors: Huang
authors: Jean-Benoit
authors: JB
authors: Charron
authors: Jae-Hoon
authors: JH
authors: Lee
authors: Gang
authors: G
authors: Li
authors: Xing Wang
authors: XW
authors: Deng
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D057906:Circadian Clocks; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; C114425:CCA1 protein, Arabidopsis; C088435:DET1 protein, Arabidopsis; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C113192:LHY protein, Arabidopsis; D009687:Nuclear Proteins; D014157:Transcription Factors