title: A plastic, disposable microfluidic flow cell for coupled on-chip PCR and microarray detection of infectious agents.
abstract: Clinical laboratories are recognizing the importance of implementing sensitive and specific molecular diagnostic tests. However, widespread adoption of these tests requires simplified workflows without requiring expensive supporting instrumentation. To enable microarray-based analysis to meet these requirements, we describe a valveless flow cell for disposable use that supports PCR coupled with microarray hybridization in the same chamber. The flow cell assembly consists simply of double-faced tape, a plastic microarray substrate, an absorbent, and a commercially-available hydrophilic thin film. The simple construction lends itself to low-cost and ease of manufacturing, yet several features reduce the complexity of the standard microarray workflow. First, there is no requirement for custom instrumentation. Second, the hydrophilic thin film allows uniform filling of a microfluidic chamber. Third, a geometric capillary stop design confines liquid to the microarray chamber during PCR, and thus eliminates the need for a valve or hydrophobic surface treatment. And fourth, imbibition drives the uniform removal of liquid reagents from the array chamber. Three hundred genomic copies of methicillin-resistant Staphylococcus aureus (MRSA) are detected in a flow cell with gel drop microarrays printed on an unmodified plastic substrate. This sensitivity is shown to be comparable to conventional methods (i.e., PCR in a tube, with separate hybridization in a microarray chamber, where amplicon is exposed to the workspace before and after hybridization). However, the flow cell combines these multiple steps into a simple, compact workflow without the need for complex valves or custom instrumentation and is less susceptible to contamination of the workspace than conventional methods because the amplicon is confined to the device.
authors: Akonni Biosystems, 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA. Cooney@akonni.com
authors: Christopher G
authors: CG
authors: Cooney
authors: David
authors: D
authors: Sipes
authors: Nitu
authors: N
authors: Thakore
authors: Rebecca
authors: R
authors: Holmberg
authors: Phillip
authors: P
authors: Belgrader
mesh_terms: D055624:Methicillin-Resistant Staphylococcus aureus / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*