title: Post-translational modifications of pro-opiomelanocrtin related hormones in medaka pituitary based on mass spectrometric analyses.
abstract: Direct tissue matrix-assisted laser desorption ionization with time-of-flight mass spectrometry analysis provides a selective detection of mass profile for the peptides contained into cell secretory granules. By this mass spectrometry with slice of pituitary, two novel molecular forms of pro-opimelanocrtin related hormone were found in the orange-red strain medaka (Oryzias latipes var.). The structures of [N,O-diacetyl Serine(1), O-acetyl Serine(3)]-α-melanocyte-stimulating hormone (MSH) and [hydroxyproline(15)]-β-MSH, together with [phosphoserine(15)]-corticotropin-like intermediate lobe peptide, were determined for the first time using a collision-induced dissociation with electrospray ionization mass spectrometry. A combination of mass spectrometry analyses is thus a powerful tool to lead to the elucidation of the post-translational processing from the pre-prohormone.
authors: Health Research Institute, National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan. yasuda-a@aist.go.jp
authors: Akikazu
authors: A
authors: Yasuda
authors: Yoshiro
authors: Y
authors: Tatsu
authors: Yoshikazu
authors: Y
authors: Kawata
authors: Toshifumi
authors: T
authors: Akizawa
authors: Yasushi
authors: Y
authors: Shigeri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008969:Molecular Sequence Data; D009990:Oryzias / Q000033:anatomy & histology / Q000378:metabolism*; D010902:Pituitary Gland / Q000737:chemistry* / Q000378:metabolism; D011333:Pro-Opiomelanocortin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D000521:alpha-MSH / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019824:beta-MSH / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D019824:beta-MSH; D000521:alpha-MSH; D011333:Pro-Opiomelanocortin