title: A ribonucleopeptide module for effective conversion of an RNA aptamer to a fluorescent sensor.
abstract: Ribonucleopeptide (RNP) is a new class of scaffold for modular fluorescent sensors. We report here a short RNA motif that induces an efficient communication between the structural changes associated with the ligand-binding event of RNA aptamer and an optical response of a fluorescent RNP module. An optimized short RNA motif was used as a communication module for the rational design of modular RNP sensors. A modular combination of a GTP-binding RNA aptamer, the short RNA motif and the fluorophore-labeled RNP module afforded a fluorescent GTP sensor that retain the ligand-binding affinity of the parent aptamer.
authors: Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors: Fong Fong
authors: FF
authors: Liew
authors: Hironori
authors: H
authors: Hayashi
authors: Shun
authors: S
authors: Nakano
authors: Eiji
authors: E
authors: Nakata
authors: Takashi
authors: T
authors: Morii
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D010455:Peptides; D012313:RNA; D000255:Adenosine Triphosphate