title: Two-photon 3D FIONA of individual quantum dots in an aqueous environment.
abstract: We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9 × 9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.
authors: Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors: Ruobing
authors: R
authors: Zhang
authors: Eli
authors: E
authors: Rothenberg
authors: Gilbert
authors: G
authors: Fruhwirth
authors: Paul D
authors: PD
authors: Simonson
authors: Fangfu
authors: F
authors: Ye
authors: Ido
authors: I
authors: Golding
authors: Tony
authors: T
authors: Ng
authors: Ward
authors: W
authors: Lopes
authors: Paul R
authors: PR
authors: Selvin
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D004705:Endocytosis; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006801:Humans; D017785:Photons; D045663:Quantum Dots; D014867:Water
chemical_list: D014867:Water; D066246:ErbB Receptors