title: Mediterranean diet reduces senescence-associated stress in endothelial cells.
abstract: This paper aims to study the effects of the oxidative stress induced by quality and quantity of dietary fat on cellular senescence. Twenty elderly subjects consumed three diets, each for 4 weeks: a saturated fatty acid diet (SFA), a low-fat and high-carbohydrate diet (CHO-ALA), and a Mediterranean diet (MedDiet) enriched in monounsaturated fatty acid following a randomized crossover design. For each diet, we investigated intracellular reactive oxidative species (ROS), cellular apoptosis and telomere length in human umbilical endothelial cells incubated with serum from each patient. MedDiet induced lower intracellular ROS production, cellular apoptosis, and percentage of cell with telomere shortening, compared with the baseline and with SFA and CHO-ALA diets. Dietary fat modulates the oxidative stress in human endothelial cells. MedDiet protects these cells from oxidative stress, prevents cellular senescence and reduces cellular apoptosis.
authors: Lipids and Atherosclerosis Unit, Córdoba, Spain.
authors: Carmen
authors: C
authors: Marin
authors: Javier
authors: J
authors: Delgado-Lista
authors: Rafael
authors: R
authors: Ramirez
authors: Julia
authors: J
authors: Carracedo
authors: Javier
authors: J
authors: Caballero
authors: Pablo
authors: P
authors: Perez-Martinez
authors: Francisco Miguel
authors: FM
authors: Gutierrez-Mariscal
authors: Antonio
authors: A
authors: Garcia-Rios
authors: Nieves
authors: N
authors: Delgado-Casado
authors: Cristina
authors: C
authors: Cruz-Teno
authors: Elena Maria
authors: EM
authors: Yubero-Serrano
authors: Francisco
authors: F
authors: Tinahones
authors: Maria del Mar
authors: Mdel M
authors: Malagon
authors: Francisco
authors: F
authors: Perez-Jimenez
authors: Jose
authors: J
authors: Lopez-Miranda
mesh_terms: D000368:Aged; D000375:Aging / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D016922:Cellular Senescence / Q000502:physiology*; D018592:Cross-Over Studies; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D038441:Diet, Mediterranean; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species; D059505:Telomere Homeostasis / Q000502:physiology
chemical_list: D004040:Dietary Carbohydrates; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D017382:Reactive Oxygen Species