title: Profiling of hormones and related metabolites in seed dormancy and germination studies.
abstract: Seed dormancy and germination are regulated by several plant hormones, such as abscisic acid, gibberellin, auxin (indole-3-acetic acid), ethylene, and brassinosteroid. Endogenous concentrations of a hormone are determined by the balance between biosynthesis and deactivation, and contribute to the regulation of physiological responses. Therefore, profiling of all hormones and their metabolites (hormonome) is a powerful approach to elucidate the regulatory networks of hormone metabolism. The methods involved in the use of liquid chromatography-electrospray ionization-tandem mass spectrometry to develop a high-sensitive and high-throughput hormonome platform are described in this chapter.
authors: Dormancy and Adaptation Research Unit, RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan. mseo@psc.riken.jp
authors: Mitsunori
authors: M
authors: Seo
authors: Yusuke
authors: Y
authors: Jikumaru
authors: Yuji
authors: Y
authors: Kamiya
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D018525:Germination / Q000235:genetics / Q000502:physiology*; D005875:Gibberellins / Q000096:biosynthesis / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D057445:Plant Dormancy; D010937:Plant Growth Regulators / Q000378:metabolism*; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D005030:Ethylenes; D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C030737:indoleacetic acid; D000040:Abscisic Acid; C036216:ethylene