title: Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis.
abstract: Experiments show that upon traumatic injury the composition of mesenteric lymph changes such that it initiates an immune response that can ultimately result in multiple organ dysfunction syndrome (MODS). To identify candidate protein mediators of this process we carried out a quantitative proteomic study on mesenteric lymph from a well characterized rat shock model. We analyzed three animals using analytical 2D differential gel electrophoresis. Intra-animal variation for the majority of protein spots was minor. Functional clustering of proteins revealed changes arising from several global classes that give novel insight into fundamental mechanisms of MODS. Mass spectrometry based proteomic analysis of proteins in mesenteric lymph can effectively be used to identify candidate mediators and loss of protective agents in shock models.
authors: Proteomics Facility, University of Colorado School of Medicine, Aurora, USA. ernest.moore@dhha.org.
authors: Ashley
authors: A
authors: Zurawel
authors: Ernest E
authors: EE
authors: Moore
authors: Erik D
authors: ED
authors: Peltz
authors: Janeen R
authors: JR
authors: Jordan
authors: Sagar
authors: S
authors: Damle
authors: Monika
authors: M
authors: Dzieciatkowska
authors: Anirban
authors: A
authors: Banerjee
authors: Kirk C
authors: KC
authors: Hansen