title: Assessment of the angiotensin-I-converting enzyme (ACE-I) inhibitory and antioxidant activities of hydrolysates of bovine brisket sarcoplasmic proteins produced by papain and characterisation of associated bioactive peptidic fractions.
abstract: The main objective was to investigate the angiotensin-I-converting enzyme (ACE-I) inhibitory and antioxidant activities of sarcoplasmic proteins isolated from the brisket muscle (Pectoralis profundus) of 3 (Bos taurus) cattle and hydrolysed with papain for 24 h at 37°C. Sarcoplasmic protein hydrolysates were ultra-filtered using molecular weight cut off (MWCO) membranes and 10-kDa and 3-kDa filtrates were obtained. The total sarcoplasmic protein extracts and the 3-kDa filtrates were tested for angiotensin I-converting enzyme inhibitory (ACE-I) activities. The total hydrolysates, 10-kDa and 3-kDa filtrates were also tested for their associated antioxidant activities using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay, the ferric ion reducing antioxidant power (FRAP) assay and the Fe(2+) metal chelating ability assay. The peptidic content of the total hydrolysates, the 10-kDa and the 3-kDa filtrates were analysed using an ORBITRAP mass spectrometer, and mass spectral data obtained were analysed using TurboSEQUEST. Eleven peptides were characterised from the total hydrolysates, fifteen from the 10-kDa filtrate fractions, whilst nine peptides were characterised from the 3-kDa filtrate fractions. Similarities between the amino acid sequences of the peptides identified in this study and previously identified antioxidant and ACE-I inhibitory peptides detailed in the BIOPEP database were outlined.
authors: Food Chemistry and Technology Department, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.
authors: Roberta
authors: R
authors: Di Bernardini
authors: Anne Maria
authors: AM
authors: Mullen
authors: Declan
authors: D
authors: Bolton
authors: Joseph
authors: J
authors: Kerry
authors: Eileen
authors: E
authors: O'Neill
authors: Maria
authors: M
authors: Hayes
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002417:Cattle; D005504:Food Analysis; D007502:Iron Chelating Agents; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism; D010206:Papain / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D012519:Sarcoplasmic Reticulum; D014867:Water
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; D010206:Papain