title: Separation of alkaloids from herbs using high-speed counter-current chromatography.
abstract: Alkaloids represent a most widespread group of bioactive natural products. Because of their alkalinity and structural diversity, the fractionation and purification of the alkaloids from herbs can often present a number of practical difficulties using the conventional chromatographic techniques. High-speed counter-current chromatography (HSCCC) is a liquid-liquid partition chromatography with a support-free liquid stationary phase, and is gaining more and more popularity as a viable separation technique for bioactive compounds from natural resources. In the present review, focus is placed on the separation of alkaloids by both conventional HSCCC and pH-zone-refining counter-current chromatography (CCC) techniques from herbs. The review presents the separation of over 120 different alkaloid compounds from more than 30 plant species by the conventional HSCCC and pH-zone-refining CCC. Based on the data from the literature, the proper solvent systems for the separation of alkaloids by the conventional HSCCC and pH-zone-refining CCC are also summarized.
authors: Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Academy of Sciences, Jinan, P. R. China.
authors: Lei
authors: L
authors: Fang
authors: Yuqin
authors: Y
authors: Liu
authors: Bin
authors: B
authors: Yang
authors: Xiao
authors: X
authors: Wang
authors: Luqi
authors: L
authors: Huang
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts