title: [Effect of ciglitazone on THP-1 macrophage CD36 expression and cholesterol influx].
abstract: OBJECTIVE
  To investigate the effect of ciglitazone on CD36 expression and cholesterol influx in THP-1 macrophage.
  
  
  METHODS
  After exposure of the cultured THP-1 macrophage to ciglitazone for 24 h, [(3)H] labeled Cholesterol influx was determined by FJ-2107P typed liquid scintillator. CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively.
  
  
  RESULTS
  PPARγ agonist, ciglitazone, elevated CD36 in both protein and mRNA levels, and increased cholesterol influx in THP-1 macrophage. The levels of cholesterol influx were 20. 3%, 28. 6%, 37. 2%, 44. 3%, 48. 7% respectively.
  
  
  CONCLUSIONS
  Our results indicated that ciglitazone may play an important role in cholesterol influx and modulating CD36 expression in THP-1 macrophage.
authors: Department of Histoplogy and Embryology, University of South China, China.
authors: Zhong-cheng
authors: ZC
authors: Mo
authors: Xin
authors: X
authors: Chen
authors: Zhi-feng
authors: ZF
authors: Long
authors: Ying
authors: Y
authors: Zeng
authors: Qing-hai
authors: QH
authors: Zhang
authors: Guang-hui
authors: GH
authors: Yi
mesh_terms: D018955:CD36 Antigens / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism*; D005487:Foam Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000819:agonists; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D018955:CD36 Antigens; D047495:PPAR gamma; D012333:RNA, Messenger; D045162:Thiazolidinediones; D002784:Cholesterol; C039671:ciglitazone