title: Insulin-like growth factor-2 antisense oligonucleotides inhibits myopia by expression blocking of retinal insulin-like growth factor-2 in guinea pig.
abstract: OBJECTIVE
  To clarify the role of IGF-2 on the development of myopia, the dynamic expression of IGF-2 was investigated in the FD eyes' retina, and the effects of intravitreous injection with IGF-2 ASON was studied on the diopter and axial eye length of FD eyes.
  
  
  METHODS
  64 guinea pigs were divided into 2 groups. In group A (n = 24), the right eyes were covered. On days 7, 14 and 21, the diopter, axial eye length and level of IGF-2 of both eyes were measured in every 8 guinea pigs. In group B (n = 40), the right eyes were covered. On day 1, the right eyes were received intravitreal injection with 40 µg IGF-2SON, 10 µg, 20 µg or 40 µg IGF-2 ASON. The diopter, axial eye length and level of IGF-2 were measured on day 14.
  
  
  RESULTS
  FD eyes showed myopic shift, axial length enlongation, and up-regulation in retinal IGF-2 from day 7 to day 21. The level of retinal IGF-2 in FD eyes was higher than that in non-FD eyes. Compare with FD eyes without injection, the myopia diopter of FD eyes decreased in received intravitreous injection with IGF-2 ASON, axial length shortened, and down-regulated with retinal IGF-2. With the increase dose of IGF-2 ASON, the change of myopic diopter, axial length, and level of retinal IGF-2 were showed more and more significant.
  
  
  CONCLUSIONS
  FD is effective to up-regulate the level of retinal IGF-2 expression in guinea pig. Intravitreous injection with IGF-2 ASON can inhibit the development of myopia.
authors: Department of Ophthalmology, Third Xiangya Hospital, Central South University, Changsha Eye Center, Changsha, China.
authors: Ren-hong
authors: RH
authors: Tang
authors: Jia
authors: J
authors: Tan
authors: Zhi-hong
authors: ZH
authors: Deng
authors: Shao-zhen
authors: SZ
authors: Zhao
authors: Ying-bin
authors: YB
authors: Miao
authors: Wei-juan
authors: WJ
authors: Zhang
mesh_terms: D000818:Animals; D058452:Axial Length, Eye; D015153:Blotting, Western; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D006168:Guinea Pigs; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D058449:Intravitreal Injections; D008081:Liposomes; D009216:Myopia / Q000378:metabolism / Q000517:prevention & control*; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D008081:Liposomes; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D007335:Insulin-Like Growth Factor II