title: C-terminal de novo sequencing of peptides using oxazolone-based derivatization with bromine signature.
abstract: Due to almost identical chemical properties of C-terminal and side-chain carboxylic groups, selective C-terminal derivatization has been difficult. Although oxazolone-based C-terminal derivatization is the only selective C-terminal modification available, it has not been used widely because of its low derivatization efficiency. In this paper, an improved oxazolone chemistry for incorporation of Br signature to C-terminus is reported. MS/MS analysis of the brominated peptides led to a series of y ions with Br signature, facilitating de novo C-terminal sequencing.
authors: Department of Chemistry, Seoul National University, Seoul 151-742, Republic of Korea. jongseo.kim1@gmail.com
authors: Jong-Seo
authors: JS
authors: Kim
authors: Mansup
authors: M
authors: Shin
authors: Jin-Su
authors: JS
authors: Song
authors: Songhie
authors: S
authors: An
authors: Hie-Joon
authors: HJ
authors: Kim
mesh_terms: D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000032:analysis / Q000737:chemistry; D001966:Bromine / Q000737:chemistry*; D008969:Molecular Sequence Data; D010081:Oxazolone / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D000804:Angiotensin II; D010081:Oxazolone; D001966:Bromine