title: Human IL-25- and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161.
abstract: Innate lymphoid cells (ILCs) are emerging as a family of effectors and regulators of innate immunity and tissue remodeling. Interleukin 22 (IL-22)- and IL-17-producing ILCs, which depend on the transcription factor RORγt, express CD127 (IL-7 receptor α-chain) and the natural killer cell marker CD161. Here we describe another lineage-negative CD127(+)CD161(+) ILC population found in humans that expressed the chemoattractant receptor CRTH2. These cells responded in vitro to IL-2 plus IL-25 and IL-33 by producing IL-13. CRTH2(+) ILCs were present in fetal and adult lung and gut. In fetal gut, these cells expressed IL-13 but not IL-17 or IL-22. There was enrichment for CRTH2(+) ILCs in nasal polyps of chronic rhinosinusitis, a typical type 2 inflammatory disease. Our data identify a unique type of human ILC that provides an innate source of T helper type 2 (T(H)2) cytokines.
authors: Tytgat Institute for Liver and Intestinal Research, University of Amsterdam, Amsterdam, The Netherlands.
authors: Jenny M
authors: JM
authors: Mjösberg
authors: Sara
authors: S
authors: Trifari
authors: Natasha K
authors: NK
authors: Crellin
authors: Charlotte P
authors: CP
authors: Peters
authors: Cornelis M
authors: CM
authors: van Drunen
authors: Berber
authors: B
authors: Piet
authors: Wytske J
authors: WJ
authors: Fokkens
authors: Tom
authors: T
authors: Cupedo
authors: Hergen
authors: H
authors: Spits
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D002908:Chronic Disease; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D000067596:Interleukin-33; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D055651:NK Cell Lectin-Like Receptor Subfamily B / Q000276:immunology / Q000378:metabolism*; D009298:Nasal Polyps; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000276:immunology / Q000378:metabolism*; D012220:Rhinitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D012852:Sinusitis / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; C486701:IL25 protein, human; C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D007378:Interleukins; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; C036293:prostaglandin D2 receptor