title: Comprehensive quality evaluation of Fructus Schisandrae using electrospray ionization ion trap multiple-stage tandem mass spectrometry coupled with chemical pattern recognition techniques.
abstract: Electrospray ionization ion trap multiple-stage tandem mass spectrometry (ESI-MS(n)) was used to evaluate Fructus Schisandrae of similar species (Schisandra chinensis (Turcz.) Baill. fruits and Schisandra sphenanthera Rehd. et Wils. fruits) and different growth characteristics (color, shape, etc.). The application of chemical pattern recognition in the ESI-MS(n) data analysis was carried out by principal component analysis (PCA), hierarchical cluster analysis (HCA) and linear discriminant analysis (LDA). Then the antioxidant activity of different Fructus Schisandrae samples were determined by an LC-ESI-MS method and ferric reducing antioxidant power (FRAP) assay. Using the ESI-MS(n) method coupled with chemical pattern recognition analysis and correlated with the antioxidant activity evaluation, the two similar species were successfully distinguished, thus improving the therapeutic safety and effectiveness. The superior characteristics of Schisandra chinensis (Turcz.) Baill. fruits were obtained and made the selection and breeding of Chinese medicine materials more scientific. This study indicates that ESI-MS(n) is a valuable tool for the authentication of botanical origin and can also be useful for the quality control of Chinese medicinal herbs.
authors: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
authors: Xin
authors: X
authors: Huang
authors: Fengrui
authors: F
authors: Song
authors: Zhiqiang
authors: Z
authors: Liu
authors: Shuying
authors: S
authors: Liu
authors: Jun
authors: J
authors: Ai
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000592:standards; D016000:Cluster Analysis; D016002:Discriminant Analysis; D004365:Drugs, Chinese Herbal; D005638:Fruit / Q000737:chemistry; D017705:Lignans / Q000032:analysis / Q000592:standards; D025341:Principal Component Analysis; D011786:Quality Control; D032141:Schisandra / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000592:standards
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D017705:Lignans