title: Human fibroblasts for large-scale "omics" investigations of ATM gene function.
abstract: ATM (gene mutated in ataxia-telangiectasia) is a critical central component of the pleiotropic responses of cells to ionizing radiation-induced stress. To gain insight into molecular mechanisms and to enhance our understanding of ATM functions, we have advanced a human model cell system, derived from genetically defined immortal fibroblasts, and we have applied high-throughput genomic, proteomic and metabolomic technologies for a systems level analysis. The cellular characterizations reported here provide the background for application of a systems analysis to integrate transcription, post-translational modifications and metabolic activity induced by exposure of cells to ionizing radiation. We present here a summary of the derivation and characterization of cells comprising this model cell system and review applications of this model to systems analysis of ATM functions.
authors: Department of Radiation Medicine, Georgetown University Medical Center, Washington, DC 20057, USA. jungm@georgetown.edu
authors: Mira
authors: M
authors: Jung
authors: Olga
authors: O
authors: Timofeeva
authors: Amrita K
authors: AK
authors: Cheema
authors: Rency
authors: R
authors: Varghese
authors: Habtom
authors: H
authors: Ressom
authors: Anatoly
authors: A
authors: Dritschilo
mesh_terms: D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005347:Fibroblasts / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D055432:Metabolomics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology; D040901:Proteomics; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000502:physiology
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases