title: Use of sentinel serology in a Bovine viral diarrhea virus eradication program.
abstract: Forty-seven cattle management groups from 36 herds in a regional Bovine viral diarrhea virus (BVDV) eradication program were selected to evaluate serology as a tool to detect herd infection with BVDV. Serum samples were obtained from 5 non-vaccinated sentinel calves ≥ 6 months old in each management group and virus neutralizing (VN) antibody titers against BVDV genotypes 1 and 2 were determined. A herd was considered positive if 2 or more sentinel calves had VN antibody titers ≥ 128 to either genotype. Results were compared to individual animal testing of all available calves by reverse transcription polymerase chain reaction (RT-PCR) on skin biopsy samples. In 1 management group from 1 herd (n = 24), 3 sentinel calves had VN antibody titers ≥ 128. Three ear notch samples from that herd were positive for BVDV on RT-PCR assay. All other management groups were negative for BVDV. In the present study, the herd sensitivity of sentinel serology was 100% (95% confidence interval [CI]: 0.05-1.0) and herd specificity was 100% (95% CI: 0.90-1.0). The κ value for agreement between sentinel serology and RT-PCR was 1.0 (95% CI: 1.0-1.0). Preliminary results suggest that sentinel animal serology can be utilized in a BVDV eradication program to provide an accurate and efficient evaluation of herd status.
authors: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, 165 NFSTC Building, Michigan State University, East Lansing, MI 48824, USA. corbett2@cvm.msu.edu
authors: Erik M
authors: EM
authors: Corbett
authors: Daniel L
authors: DL
authors: Grooms
authors: Steven R
authors: SR
authors: Bolin
authors: Ben
authors: B
authors: Bartlett
authors: Dale M
authors: DM
authors: Grotelueschen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000517:prevention & control*; D002417:Cattle / Q000821:virology; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics; D060740:Disease Eradication / Q000379:methods; D005838:Genotype; D009500:Neutralization Tests / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D018571:Sentinel Surveillance / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral