title: Direct and indirect organogenesis of Alpinia galanga and the phytochemical analysis.
abstract: Alpinia galanga is a rhizomatous herb rich in essential oils and various other significant phytoconstituents. Rapid direct regeneration was obtained from the rhizome explants (15.66 ± 0.57 shoots) on MS media supplemented with zeatin at a concentration of 2 mg/l. The callus cultures of A. galanga were initiated from the rhizome explants on MS media supplemented with 2 mg/l each of BAP, 2,4-D, and NAA. The callus was analyzed for the presence of a vital phytoconstituent--acetoxychavicol acetate (ACA) associated with various biological properties. ACA was detected in the young friable callus as well as the stationary phase callus. Moreover, the induction of morphogenetic response in callus resulted in higher accumulation of ACA. The phytohormone withdrawal from the propagation media and the subsequent transfer of callus to BAP (2 mg/l) containing MS media has resulted in multiple shoot induction. The regenerated (indirect) plants have shown 1.6-fold higher ACA content (1.253%) when compared to the control plant (0.783%). Micropropagation of such conventionally propagated plants is very essential to meet the commercial demand as well as to ensure easy storage and transportation of disease free stocks.
authors: Centre for Biotechnology, Institute of Science & Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad 500 085, India. raorskiran@rediffmail.com
authors: Kiranmayee
authors: K
authors: Rao
authors: Bhuvaneswari
authors: B
authors: Chodisetti
authors: Suryakala
authors: S
authors: Gandi
authors: Lakshmi Narasu
authors: LN
authors: Mangamoori
authors: Archana
authors: A
authors: Giri
mesh_terms: D032429:Alpinia / Q000737:chemistry* / Q000196:embryology* / Q000502:physiology; D009024:Morphogenesis; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D012038:Regeneration; D027343:Rhizome / Q000737:chemistry / Q000196:embryology; D046509:Tissue Culture Techniques
chemical_list: D010936:Plant Extracts