title: Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen.
abstract: This study is focusing on elucidation of the capacity of attenuated Salmonella enteritidis E23 (cya, crp) to serve as a vehicle for the rectal delivery of the DNA vaccine. Earlier for creation HIV-1 candidate DNA vaccine we have designed the polyepitope protein TCI (T-cell immunogen), which comprises over 80 CTL epitopes from subtype A, B and C HIV-1 proteins. The gene coding for TCI protein was used to construct the eukaryotic expression plasmid pcDNA-TCI. The attenuated S. enteritidis E23 was transformed by electroporation with recombinant plasmid pcDNA-TCI and the expression of the TCI gene was determined in vitro and in vivo. BALB/c mice were rectally immunized with S. enteritidis E23/pcDNA-TCI (10⁸ cfu) twice at 4 week interval. Bacteria were not pathogenic for mice and spontaneously eliminated from mice spleen and liver to 60 days post the immunization. Detectable antibodies were generated in 2 weeks after immunization and their level increased after second immunization. The results of INF-γ ELISpot show that mice immunized with S. enteritidis E23/pcDNA-TCI elicited HIV-specific cellular immune response. This study demonstrates that attenuated S. enteritidis E23 is an effective live vector for rectal delivery of the DNA vaccine pcDNA-TCI to generate humoral and T-cellular responses against HIV-1.
authors: State Research Center of Virology and Biotechnology 'Vector', 630559 Koltsovo, Novosibirsk, Russia. lkarpenko@ngs.ru
authors: Larisa I
authors: LI
authors: Karpenko
authors: Alexey V
authors: AV
authors: Danilenko
authors: Sergei I
authors: SI
authors: Bazhan
authors: Elena D
authors: ED
authors: Danilenko
authors: Galina M
authors: GM
authors: Sysoeva
authors: Olga N
authors: ON
authors: Kaplina
authors: Olga Y
authors: OY
authors: Volkova
authors: Svetlana F
authors: SF
authors: Oreshkova
authors: Alexander A
authors: AA
authors: Ilyichev
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D000285:Administration, Rectal; D000818:Animals; D016503:Drug Delivery Systems; D058501:Enzyme-Linked Immunospot Assay; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D015483:HIV Antibodies / Q000097:blood; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012477:Salmonella enteritidis / Q000235:genetics*; D013154:Spleen / Q000382:microbiology; D014611:Vaccination / Q000379:methods; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
chemical_list: D016915:AIDS Vaccines; D018984:Epitopes, T-Lymphocyte; D015483:HIV Antibodies; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D007371:Interferon-gamma