title: Salvage and storage of infectious disease protein targets in the SSGCID high-throughput crystallization pathway using microfluidics.
abstract: The MPCS Plug Maker is a microcapillary-based protein-crystallization system for generating diffraction-ready crystals from nanovolumes of protein. Crystallization screening using the Plug Maker was used as a salvage pathway for proteins that failed to crystallize during the initial observation period using the traditional sitting-drop vapor-diffusion method. Furthermore, the CrystalCards used to store the crystallization experiments set up by the Plug Maker are shown be a viable container for long-term storage of protein crystals without a discernable loss of diffraction quality with time. Use of the Plug Maker with SSGCID proteins is demonstrated to be an effective crystal-salvage and storage method.
authors: Emerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA.
authors: Jeff
authors: J
authors: Christensen
authors: Cory J
authors: CJ
authors: Gerdts
authors: Mathew C
authors: MC
authors: Clifton
authors: Lance
authors: L
authors: Stewart
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D044085:Microfluidics / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins