title: Identification of nontuberculous mycobacteria isolated from clinical sterile sites in patients at a university hospital in the city of Rio de Janeiro, Brazil.
abstract: OBJECTIVE
  To identify nontuberculous mycobacteria (NTM) isolated from sterile sites in patients hospitalized between 2001 and 2006 at the Clementino Fraga Filho University Hospital, located in the city of Rio de Janeiro, Brazil.
  
  
  METHODS
  During the study period, 34 NTM isolates from sterile sites of 14 patients, most of whom were HIV-positive, were submitted to phenotypic identification and hsp65 PCR-restriction enzyme analysis (PRA).
  
  
  RESULTS
  Most isolates were identified as Mycobacterium avium, followed by M. monacense, M. kansasii, and M. abscessus.
  
  
  CONCLUSIONS
  The combination of PRA, a relatively simple and inexpensive method, with the evaluation of a few phenotypic characteristics can allow NTM to be accurately identified in the routine of clinical laboratories.
authors: Federal University of Rio de Janeiro – Rio de Janeiro, Brazil. simonegsenna@yahoo.com.br
authors: Simone Gonçalves
authors: SG
authors: Senna
authors: Ana Grazia
authors: AG
authors: Marsico
authors: Gisele Betzler de Oliveira
authors: GB
authors: Vieira
authors: Luciana Fonseca
authors: LF
authors: Sobral
authors: Philip Noel
authors: PN
authors: Suffys
authors: Leila de Souza
authors: Lde S
authors: Fonseca
mesh_terms: D000293:Adolescent; D000328:Adult; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques; D001938:Brazil; D018834:Chaperonin 60 / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial / Q000235:genetics*; D006785:Hospitals, University; D006801:Humans; D007297:Inpatients; D008875:Middle Aged; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology*; D015269:Mycobacterium avium Complex / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods*; D055815:Young Adult
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D004262:DNA Restriction Enzymes