title: Potential for chemolithoautotrophy among ubiquitous bacteria lineages in the dark ocean.
abstract: Recent studies suggest that unidentified prokaryotes fix inorganic carbon at globally significant rates in the immense dark ocean. Using single-cell sorting and whole-genome amplification of prokaryotes from two subtropical gyres, we obtained genomic DNA from 738 cells representing most cosmopolitan lineages. Multiple cells of Deltaproteobacteria cluster SAR324, Gammaproteobacteria clusters ARCTIC96BD-19 and Agg47, and some Oceanospirillales from the lower mesopelagic contained ribulose-1,5-bisphosphate carboxylase-oxygenase and sulfur oxidation genes. These results corroborated community DNA and RNA profiling from diverse geographic regions. The SAR324 genomes also suggested C(1) metabolism and a particle-associated life-style. Microautoradiography and fluorescence in situ hybridization confirmed bicarbonate uptake and particle association of SAR324 cells. Our study suggests potential chemolithoautotrophy in several uncultured Proteobacteria lineages that are ubiquitous in the dark oxygenated ocean and provides new perspective on carbon cycling in the ocean's largest habitat.
authors: Bigelow Laboratory for Ocean Sciences, 180 McKown Point Road, Post Office Box 475, West Boothbay Harbor, ME 04575, USA.
authors: Brandon K
authors: BK
authors: Swan
authors: Manuel
authors: M
authors: Martinez-Garcia
authors: Christina M
authors: CM
authors: Preston
authors: Alexander
authors: A
authors: Sczyrba
authors: Tanja
authors: T
authors: Woyke
authors: Dominique
authors: D
authors: Lamy
authors: Thomas
authors: T
authors: Reinthaler
authors: Nicole J
authors: NJ
authors: Poulton
authors: E Dashiell P
authors: ED
authors: Masland
authors: Monica Lluesma
authors: ML
authors: Gomez
authors: Michael E
authors: ME
authors: Sieracki
authors: Edward F
authors: EF
authors: DeLong
authors: Gerhard J
authors: GJ
authors: Herndl
authors: Ramunas
authors: R
authors: Stepanauskas
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D057486:Carbon Cycle; D002245:Carbon Dioxide / Q000378:metabolism; D052837:Chemoautotrophic Growth; D003624:Darkness; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D054892:Metagenome; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010084:Oxidation-Reduction; D010802:Phylogeny; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D013455:Sulfur / Q000378:metabolism; D013457:Sulfur Compounds / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D013457:Sulfur Compounds; D002245:Carbon Dioxide; D013455:Sulfur; D012273:Ribulose-Bisphosphate Carboxylase