title: Nitric oxide methods in seed biology.
abstract: The ubiquitous signaling molecule nitric oxide (NO) plays an important role in seed biology. Experiments with this biologically important gas require special provisions because NO in aerobic environments is readily converted into other oxides of nitrogen. In this chapter, we describe methods for the application of NO as a gas, and through the use of NO-donor compounds. We included information on the removal or reduction of NO with NO scavengers. Methods for detecting NO using NO-reactive fluorescent probes, and an apparatus incorporating an oxidizer column are also described.
authors: Department of Horticulture, University of Wisconsin-Madison, Madison, WI, USA. paul.bethke@ars.usda.gov
authors: Paul C
authors: PC
authors: Bethke
authors: Igor G L
authors: IG
authors: Libourel
authors: Jan
authors: J
authors: Vitecek
authors: Russell L
authors: RL
authors: Jones
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D015920:Gas Scavengers; D018525:Germination / Q000235:genetics*; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism*; D009573:Nitrites / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D057445:Plant Dormancy / Q000235:genetics*; D012639:Seeds / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D009566:Nitrates; D009573:Nitrites; D009569:Nitric Oxide