title: [A study on the construction, expression and immunosterility of Lagurus laguru zona pellucida 3 DNA vaccine pVAX1-sig-LTB-lZP3-C3d3].
abstract: OBJECTIVE
  To enhance the immunocontraceptive effect of Lagurus lagurus zona pellucida 3 DNA vaccine, and to achieve the prospect of application through the pVAX1-sig-LTB-lZP3-C3d3 different immunity pathway.
  
  
  METHODS
  Two adjuvant molecules were constructed into the recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 as DNA vaccine which contains Escherichia coli heat-labile enterotoxin B subunit and the molecular adjuvant 3 copies of C3d. The results of RT-PCR and western blot showed that the DNA vaccine was expressed in mRNA and protein level. The female C57BL/6 mice were immunized by three ways: intramuscular injection, intranasal or oral route.Antibody levels and types were detected by ELISA.
  
  
  RESULTS
  ELISA results showed that recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 immunization induced specific IgG, IgA levels were significantly different comparing with control (P<0.01). Antifertility experiment showed that the experimental group reduced the average fertility significantly different compared with the control group (P<0.01).
  
  
  CONCLUSIONS
  Restriction analysis, RT-PCR and Western blot showed that the recombinant plasmid constructed correctly and can be the expression of mRNA and protein levels.It resulted that the recombinant plasmid pVAX1-sig-LTB-lZP3-C3d3 can induce the specific immune response efficiently and enhance the immunocontraceptive effects.
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
authors: Chen-Chen
authors: CC
authors: Li
authors: Ji-Yun
authors: JY
authors: Yu
authors: Min
authors: M
authors: Jiang
authors: Yi-Xian
authors: YX
authors: Tu
authors: Xiao-Lin
authors: XL
authors: Ma
authors: Fu-Chun
authors: FC
authors: Zhang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D003411:Arvicolinae; D003269:Contraception, Immunologic; D004527:Egg Proteins / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007247:Infertility, Female / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000276:immunology*; D022642:Vaccines, Contraceptive / Q000235:genetics* / Q000276:immunology*; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D057134:Antibodies, Neutralizing; D004527:Egg Proteins; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D022642:Vaccines, Contraceptive; D019444:Vaccines, DNA; D000072216:Zona Pellucida Glycoproteins