title: Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method.
abstract: BACKGROUND
  Capillary electrophoresis (CE) is a high-resolution method for detection of hemoglobin Constant Spring (Hb CS).
  
  
  METHODS
  The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H-CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups.
  
  
  RESULTS
  Mean ± SD of Hb CS level of the homozygote was not significantly different from that of the Hb H-CS disease (1.9 ± 1.8 vs. 2.8 ± 1.3, P = 0.13), but it was significantly higher than that of the heterozygote (1.9 ± 1.8 vs. 0.4 ± 0.2, P = 0.007). The MCV <70 fL was found in Hb H-CS disease only.
  
  
  CONCLUSIONS
  CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70 fL), this approach provided a high resolution to identify Hb H-CS disease.
authors: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang-Mai University, Chiang-Mai, Thailand. sakornmi001@gmail.com
authors: S
authors: S
authors: Pornprasert
authors: S
authors: S
authors: Panyasai
authors: J
authors: J
authors: Waneesorn
authors: K
authors: K
authors: Kongthai
authors: P
authors: P
authors: Singboottra
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D004909:Erythrocyte Indices / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000032:analysis* / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D017085:alpha-Thalassemia / Q000175:diagnosis*
chemical_list: D006455:Hemoglobins, Abnormal; C035541:Hemoglobin Constant Spring