title: Ultrasound-assisted extraction of flaxseed oil using immobilized enzymes.
abstract: An aqueous enzymatic process assisted by ultrasound extraction (AEP-UE) was applied to the extraction of oil from flaxseed (Linum usitatissimum L.). The highest oil recovery of 68.1% was obtained when ground flaxseed was incubated with 130 U/g of cellulase, pectinase, and hemicellulase for 12h, at 45°C and pH 5.0. The IC(50) values of oil obtained by AEP-UE and organic solvent extraction (OSE), as measured by DPPH scavenging activity essay, were 2.27 mg/mL and 3.31 mg/mL. The AEP-UE-derived oil had a 1.5% higher content of unsaturated fatty acids than the OSE-derived oil. AEP-UE is therefore a promising environmentally friendly method for large-scale preparation of flaxseed oil.
authors: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
authors: Jing-jing
authors: JJ
authors: Long
authors: Yu-jie
authors: YJ
authors: Fu
authors: Yuan-gang
authors: YG
authors: Zu
authors: Ji
authors: J
authors: Li
authors: Wei
authors: W
authors: Wang
authors: Cheng-bo
authors: CB
authors: Gu
authors: Meng
authors: M
authors: Luo
mesh_terms: D001713:Biphenyl Compounds / Q000378:metabolism; D002480:Cellulase / Q000378:metabolism; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006026:Glycoside Hydrolases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D008043:Linseed Oil / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism; D025341:Principal Component Analysis; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics / Q000379:methods*
chemical_list: D001713:Biphenyl Compounds; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D016166:Free Radical Scavengers; D010851:Picrates; D008043:Linseed Oil; C004931:1,1-diphenyl-2-picrylhydrazyl; D006026:Glycoside Hydrolases; C023305:hemicellulase; D011096:Polygalacturonase; D002480:Cellulase