title: Utilization of glycosyltransferases for the synthesis of a densely packed hyperbranched polysaccharide brush coating as artificial glycocalyx.
abstract: Densely packed polysaccharide brushes consisting of α-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase and Deinococcusgeothermalis branching enzyme resulted in a hyperbranched brush coating as the latter one redistributes short oligosaccharides from the α(1-4)-linked position to the α (1-6)-linked position in the polysaccharide brush. The obtained grafting density of the brushes was estimated on 1.89 nm(-2) while the thickness was measured with ellipsometric techniques and determined to be between 12.2 and 20.2 nm.
authors: Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
authors: Jeroen
authors: J
authors: van der Vlist
authors: Iris
authors: I
authors: Schönen
authors: Katja
authors: K
authors: Loos
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000378:metabolism; D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism; D034301:Deinococcus / Q000737:chemistry / Q000201:enzymology*; D005936:Glucans / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism; D019276:Glycocalyx / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006005:Phosphorylases / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants / Q000382:microbiology; D012825:Silicon / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005936:Glucans; D005958:Glucosephosphates; C031146:maltoheptaose; C031590:glucose-1-phosphate; D006005:Phosphorylases; D015061:1,4-alpha-Glucan Branching Enzyme; D012825:Silicon