title: Transillumination hyperspectral imaging for histopathological examination of excised tissue.
abstract: Angular domain spectroscopic imaging (ADSI) is a novel technique for the detection and characterization of optical contrast in turbid media based on spectral characteristics. The imaging system employs a silicon micromachined angular filter array to reject scattered light traversing a specimen and an imaging spectrometer to capture and discriminate the largely remaining quasiballistic light based on spatial position and wavelength. The imaging modality results in hyperspectral shadowgrams containing two-dimensional (2D) spatial maps of spectral information. An ADSI system was constructed and its performance was evaluated in the near-infrared region on tissue-mimicking phantoms. Image-based spectral correlation analysis using transmission spectra and first order derivatives revealed that embedded optical targets could be resolved. The hyperspectral images obtained with ADSI were observed to depend on target concentration, target depth, and scattering level of the background medium. A similar analysis on a muscle and tumor sample dissected from a mouse resulted in spatially dependent optical transmission spectra that were distinct, which suggested that ADSI may find utility in classifying tissues in biomedical applications.
authors: Lawson Health Research Institute, Imaging Program, London, Ontario, Canada N6A 4V2.
authors: Fartash
authors: F
authors: Vasefi
authors: Mohamadreza
authors: M
authors: Najiminaini
authors: Eldon
authors: E
authors: Ng
authors: Astrid
authors: A
authors: Chamson-Reig
authors: Bozena
authors: B
authors: Kaminska
authors: Muriel
authors: M
authors: Brackstone
authors: Jeffrey
authors: J
authors: Carson
mesh_terms: D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007208:Indocyanine Green / Q000737:chemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D018482:Muscle, Skeletal / Q000737:chemistry; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000737:chemistry; D019047:Phantoms, Imaging; D012815:Signal Processing, Computer-Assisted; D013057:Spectrum Analysis / Q000379:methods*; D014172:Transillumination / Q000379:methods*
chemical_list: D007208:Indocyanine Green