title: Phage displayed peptides to avian H5N1 virus distinguished the virus from other viruses.
abstract: The purpose of the current study was to identify potential ligands and develop a novel diagnostic test to highly pathogenic avian influenza A virus (HPAI), subtype H5N1 viruses using phage display technology. The H5N1 viruses were used as an immobilized target in a biopanning process using a 12-mer phage display random peptide library. After five rounds of panning, three phages expressing peptides HAWDPIPARDPF, AAWHLIVALAPN or ATSHLHVRLPSK had a specific binding activity to H5N1 viruses were isolated. Putative binding motifs to H5N1 viruses were identified by DNA sequencing. In terms of the minimum quantity of viruses, the phage-based ELISA was better than antiserum-based ELISA and a manual, semi-quantitative endpoint RT-PCR for detecting H5N1 viruses. More importantly, the selected phages bearing the specific peptides to H5N1 viruses were capable of differentiating this virus from other avian viruses in enzyme-linked immunosorbent assays.
authors: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
authors: Dan
authors: D
authors: Wu
authors: Guangxing
authors: G
authors: Li
authors: Chengfeng
authors: C
authors: Qin
authors: Xiaofeng
authors: X
authors: Ren
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001435:Bacteriophages / Q000235:genetics; D001717:Birds / Q000276:immunology / Q000821:virology*; D002460:Cell Line; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D005585:Influenza in Birds / Q000821:virology; D057230:Limit of Detection; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D000914:Antibodies, Viral; D019151:Peptide Library; D010455:Peptides