title: A new model for the estimation of cell proliferation dynamics using CFSE data.
abstract: CFSE analysis of a proliferating cell population is a popular tool for the study of cell division and divisionlinked changes in cell behavior. Recently Banks et al. (2011), Luzyanina et al. (2009), Luzyanina et al. (2007), a partial differential equation (PDE) model to describe lymphocyte dynamics in a CFSE proliferation assay was proposed. We present a significant revision of this model which improves the physiological understanding of several parameters. Namely, the parameter used previously as a heuristic explanation for the dilution of CFSE dye by cell division is replaced with a more physical component, cellular autofluorescence. The rate at which label decays is also quantified using a Gompertz decay process. We then demonstrate a revised method of fitting the model to the commonly used histogram representation of the data. It is shown that these improvements result in a model with a strong physiological basis which is fully capable of replicating the behavior observed in the data.
authors: Center for Research in Scientific Computation, North Carolina State University, Raleigh, NC 27695-8212, USA. htbanks@ncsu.edu
authors: H T
authors: HT
authors: Banks
authors: Karyn L
authors: KL
authors: Sutton
authors: W Clayton
authors: WC
authors: Thompson
authors: Gennady
authors: G
authors: Bocharov
authors: Marie
authors: M
authors: Doumic
authors: Tim
authors: T
authors: Schenkel
authors: Jordi
authors: J
authors: Argilaguet
authors: Sandra
authors: S
authors: Giest
authors: Cristina
authors: C
authors: Peligero
authors: Andreas
authors: A
authors: Meyerhans
mesh_terms: D000465:Algorithms; D002455:Cell Division; D049109:Cell Proliferation; D005434:Flow Cytometry; D005452:Fluoresceins / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008954:Models, Biological; D056004:Molecular Dynamics Simulation; D013388:Succinimides / Q000378:metabolism*
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013388:Succinimides