title: Rapid, massively parallel single-cell drug response measurements via live cell interferometry.
abstract: A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Here, we develop live cell interferometry for rapid, real-time quantification of cell mass in cells exposed to a changing environment. We used tunicamycin induction of the unfolded protein stress response in multiple myeloma cells to generate a mass response that was temporally profiled for hundreds of cells simultaneously. Within 2 h, the treated cells were growth suppressed compared to controls, with a few cells in both populations showing a robust increase (+15%) or little change (<5%) in mass accumulation. Overall, live cell interferometry provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs.
authors: California NanoSystems Institute, David Geffen School of Medicine, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, California, USA. jreed@cnsi.ucla.edu
authors: Jason
authors: J
authors: Reed
authors: Jennifer
authors: J
authors: Chun
authors: Thomas A
authors: TA
authors: Zangle
authors: Sheraz
authors: S
authors: Kalim
authors: Jason S
authors: JS
authors: Hong
authors: Sarah E
authors: SE
authors: Pefley
authors: Xin
authors: X
authors: Zheng
authors: James K
authors: JK
authors: Gimzewski
authors: Michael A
authors: MA
authors: Teitell
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D007368:Interferometry / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D013997:Time Factors; D014415:Tunicamycin / Q000494:pharmacology*; D056811:Unfolded Protein Response / Q000187:drug effects
chemical_list: D014415:Tunicamycin