title: Prior knowledge of HPV status improves detection of CIN2+ by cytology screening.
abstract: OBJECTIVE
  The objective of the study was to investigate whether knowledge of human papillomavirus (HPV) deoxyribonucleic acid test results increases sensitivity of guided cytology screening for the detection of cervical intraepithelial neoplasia (CIN)-2 or higher-grade cervical lesions.
  
  
  METHODS
  This was a prospective colposcopy-controlled study of 2905 BD SurePath samples to identify cases with CIN2+ within a 24 month follow-up period. Sensitivity and specificity to detect CIN2+ was evaluated, comparing guided cytology screening with and without prior knowledge of HPV status.
  
  
  RESULTS
  Prior knowledge of HPV status resulted in significantly higher detection rate of CIN2+ compared with screening blinded to HPV status (P = .005) with limited loss of specificity (P = .026). Gain in sensitivity is higher in older women (43.8%, P = .008) vs in younger women (10.2%, P = .317), whereas loss of specificity is more pronounced in younger women (P < .001) vs older women (P = .729).
  
  
  CONCLUSIONS
  Guided cytological screening performed with prior knowledge of HPV status results in an improved detection of CIN2 or higher-grade lesions.
authors: Department of Molecular Pathology (RIATOL) Sonic Healthcare, Antwerp, Belgium. ina.benoy@riatol.be
authors: Ina H
authors: IH
authors: Benoy
authors: Davy
authors: D
authors: Vanden Broeck
authors: Maya J
authors: MJ
authors: Ruymbeke
authors: Shaira
authors: S
authors: Sahebali
authors: Marc
authors: M
authors: Arbyn
authors: Johannes J
authors: JJ
authors: Bogers
authors: Marleen
authors: M
authors: Temmerman
authors: Christophe E
authors: CE
authors: Depuydt
mesh_terms: D000328:Adult; D000367:Age Factors; D003127:Colposcopy / Q000592:standards; D003581:Cytodiagnosis / Q000592:standards; D015344:DNA Probes, HPV; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D007722:Health Knowledge, Attitudes, Practice; D006801:Humans; D008403:Mass Screening / Q000592:standards*; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000150:complications / Q000473:pathology*; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology
chemical_list: D015344:DNA Probes, HPV