title: Immunoproteomic assay of secreted proteins of Streptococcus suis serotype 9 with convalescent sera from pigs.
abstract: Streptococcus suis is an important pathogen of pigs. In China, in addition to S. suis serotype 2, S. suis serotype 9 (SS9) is also a prevalent serotype. There is no vaccine available for SS9. An immunoproteome-based approach was developed to identify SS9 immunogenic proteins for vaccine development. Secreted proteins extracted from SS9 strain GZ0565 were screened by two-dimensional Western blotting using convalescent sera from pigs. Protein spots were excised from preparative gels and were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which led to the identification of ten immunogenic proteins (sortases, ABC transporter substrate-binding protein-maltose/maltodextrin, ABC transporter periplasmic protein, CHAP domain containing protein, peptidoglycan-binding LysM, elongation factor Tu, elongation factor G, thymidine kinase, molecular chaperone DnaK, hypothetical protein SSU98_2184). These novel immunogenic proteins, which are encoded by genes that are reasonably conserved among SS9 strains, may be developed as antigens for further study of SS9 vaccine.
authors: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors: Zongfu
authors: Z
authors: Wu
authors: Wei
authors: W
authors: Zhang
authors: Jing
authors: J
authors: Shao
authors: Yang
authors: Y
authors: Wang
authors: Yan
authors: Y
authors: Lu
authors: Chengping
authors: C
authors: Lu
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D002681:China; D003289:Convalescence; D015180:Electrophoresis, Gel, Two-Dimensional; D040901:Proteomics / Q000379:methods; D044967:Serum / Q000737:chemistry / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013290:Streptococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control* / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000096:biosynthesis / Q000627:therapeutic use; D017011:Streptococcus suis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D022541:Streptococcal Vaccines