title: Three different spectrophotometric methods manipulating ratio spectra for determination of binary mixture of Amlodipine and Atorvastatin.
abstract: Three simple, specific, accurate and precise spectrophotometric methods manipulating ratio spectra are developed for the simultaneous determination of Amlodipine besylate (AM) and Atorvastatin calcium (AT) in tablet dosage forms. The first method is first derivative of the ratio spectra ((1)DD), the second is ratio subtraction and the third is the method of mean centering of ratio spectra. The calibration curve is linear over the concentration range of 3-40 and 8-32 μg/ml for AM and AT, respectively. These methods are tested by analyzing synthetic mixtures of the above drugs and they are applied to commercial pharmaceutical preparation of the subjected drugs. Standard deviation is <1.5 in the assay of raw materials and tablets. Methods are validated as per ICH guidelines and accuracy, precision, repeatability and robustness are found to be within the acceptable limit.
authors: Cairo University, Faculty of Pharmacy, Department of Analytical Chemistry, Cairo, Egypt. hdarwish75@yahoo.com
authors: Hany W
authors: HW
authors: Darwish
authors: Said A
authors: SA
authors: Hassan
authors: Maissa Y
authors: MY
authors: Salem
authors: Badr A
authors: BA
authors: El-Zeiny
mesh_terms: D017311:Amlodipine / Q000032:analysis*; D000924:Anticholesteremic Agents / Q000032:analysis*; D000959:Antihypertensive Agents / Q000032:analysis*; D000069059:Atorvastatin; D006538:Heptanoic Acids / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D011758:Pyrroles / Q000032:analysis*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*; D013607:Tablets
chemical_list: D000924:Anticholesteremic Agents; D000959:Antihypertensive Agents; D006538:Heptanoic Acids; D004364:Pharmaceutical Preparations; D011758:Pyrroles; D013607:Tablets; D017311:Amlodipine; D000069059:Atorvastatin