title: The 8-hydroxydeoxyguanosine concentrations according to hormone therapy and S326C polymorphism of OGG1 gene in postmenopausal women.
abstract: BACKGROUND
  The 8-hydroxydeoxyguanosine (8-OHdG) is widely used for determination of DNA damage since it is excised from oxidative damaged DNA with endonuclease repair enzymes coded by 8-oxoguanine DNA N-glycosylase gene (OGG1). The present study aimed at investigating whether hormone therapy (HT) may influence on the blood/urinary 8-OHdG levels and whether the level of 8-OHdG is different according to OGG1 S326C polymorphism in postmenopausal women receiving HT.
  
  
  METHODS
  In 102 postmenopausal women receiving HT, the 8-OHdG levels were measured in the blood and urine using high performance liquid chromatography (HPLC) before HT and 3 months after HT. The genotyping of the S326C polymorphism of the OGG1 was performed by polymerase chain reaction (PCR) and restriction enzyme fragment length polymorphism (RFLP) analysis.
  
  
  RESULTS
  After HT, mean blood 8-OHdG level significantly decreased compared to those before HT (P=0.003), while urinary 8-OHdG level did not show any difference according to HT. Pre-HT level of 8-OHdG was not different according to OGG1 genotypes and similar finding was demonstrated in post-HT 8-OHdG concentration.
  
  
  CONCLUSIONS
  These findings imply that hormone therapy can reduce blood 8-OHdG concentration, one of the markers of oxidative damage. Further study is needed to confirm this association in larger population.
authors: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Hoon
authors: H
authors: Kim
authors: Seung-Yup
authors: SY
authors: Ku
authors: Jong Won
authors: JW
authors: Kang
authors: Heon
authors: H
authors: Kim
authors: Yong Dae
authors: YD
authors: Kim
authors: Seok Hyun
authors: SH
authors: Kim
authors: Young Min
authors: YM
authors: Choi
authors: Jung Gu
authors: JG
authors: Kim
authors: Shin Yong
authors: SY
authors: Moon
mesh_terms: D019943:Amino Acid Substitution; D054458:Amplified Fragment Length Polymorphism Analysis; D015415:Biomarkers / Q000097:blood / Q000652:urine; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D006151:Guanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D020249:Hormone Replacement Therapy; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D017698:Postmenopause / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D006151:Guanosine; C046215:8-hydroxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human