title: Very-long-chain fatty acids are required for cell plate formation during cytokinesis in Arabidopsis thaliana.
abstract: Acyl chain length is thought to be crucial for biophysical properties of the membrane, in particular during cell division, when active vesicular fusion is necessary. In higher plants, the process of cytokinesis is unique, because the separation of the two daughter cells is carried out by de novo vesicular fusion to generate a laterally expanding cell plate. In Arabidopsis thaliana, very-long-chain fatty acid (VLCFA) depletion caused by a mutation in the microsomal elongase gene PASTICCINO2 (PAS2) or by application of the selective elongase inhibitor flufenacet altered cytokinesis. Cell plate expansion was delayed and the formation of the endomembrane tubular network altered. These defects were associated with specific aggregation of the cell plate markers YFP-Rab-A2a and KNOLLE during cytokinesis. Changes in levels of VLCFA also resulted in modification of endocytosis and sensitivity to brefeldin A. Finally, the cytokinesis impairment in pas2 cells was associated with reduced levels of very long fatty acyl chains in phospholipids. Together, our findings demonstrate that VLCFA-containing lipids are essential for endomembrane dynamics during cytokinesis.
authors: Institut Jean-Pierre Bourgin (IJPB), UMR1318 INRA-AgroParisTech, Saclay Plant Science (SPS), INRA Centre de Versailles-Grignon, Route de St-Cyr, 78000 Versailles, France.
authors: Liên
authors: L
authors: Bach
authors: Lionel
authors: L
authors: Gissot
authors: Jessica
authors: J
authors: Marion
authors: Frédérique
authors: F
authors: Tellier
authors: Patrick
authors: P
authors: Moreau
authors: Béatrice
authors: B
authors: Satiat-Jeunemaître
authors: Jean-Christophe
authors: JC
authors: Palauqui
authors: Johnathan A
authors: JA
authors: Napier
authors: Jean-Denis
authors: JD
authors: Faure
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020126:Brefeldin A / Q000494:pharmacology; D048750:Cell Nucleus Division; D048749:Cytokinesis; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D004705:Endocytosis; D005227:Fatty Acids / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009154:Mutation; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000648:ultrastructure; D050765:Qa-SNARE Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D005227:Fatty Acids; C097724:KNOLLE protein, Arabidopsis; C477363:PEPINO protein, Arabidopsis; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D020126:Brefeldin A