title: Yokukansan enhances pentobarbital-induced sleep in socially isolated mice: possible involvement of GABA(A)-benzodiazepine receptor complex.
abstract: In the present study, we investigated the effect of the Kampo medicine Yokukansan (YKS) on pentobarbital-induced sleep in group-housed and socially isolated mice. Socially isolated mice showed shorter sleeping time than the group-housed mice. YKS (300 mg/kg, p.o.) prolonged the pentobarbital-induced sleeping time in socially isolated mice without affecting pentobarbital sleep in group-housed mice. The prolongation of sleeping time by YKS was reversed by bicuculline (3 mg/kg, i.p.) and flumazenil (3 mg/kg, i.p.), but not WAY100635. These findings suggest that the GABA(A)-benzodiazepine receptor complex, but not 5-HT(1A) receptors, is involved in the reversal effect of YKS on the decrease of pentobarbital sleep by social isolation.
authors: Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka 814-0180, Japan. n-egashi@pharm.med.kyushu-u.ac.jp
authors: Nobuaki
authors: N
authors: Egashira
authors: Ai
authors: A
authors: Nogami
authors: Katsunori
authors: K
authors: Iwasaki
authors: Ayumi
authors: A
authors: Ishibashi
authors: Naoki
authors: N
authors: Uchida
authors: Kotaro
authors: K
authors: Takasaki
authors: Kenichi
authors: K
authors: Mishima
authors: Ryoji
authors: R
authors: Nishimura
authors: Ryozo
authors: R
authors: Oishi
authors: Michihiro
authors: M
authors: Fujiwara
mesh_terms: D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000627:therapeutic use*; D005442:Flumazenil / Q000494:pharmacology; D058785:GABA-A Receptor Agonists / Q000627:therapeutic use; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D006993:Hypnotics and Sedatives / Q000627:therapeutic use*; D008297:Male; D020835:Medicine, Kampo; D051379:Mice; D008815:Mice, Inbred Strains; D010424:Pentobarbital / Q000627:therapeutic use*; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism*; D012890:Sleep / Q000187:drug effects*; D012893:Sleep Wake Disorders / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D012934:Social Isolation; D013315:Stress, Psychological / Q000503:physiopathology
chemical_list: D004365:Drugs, Chinese Herbal; D058785:GABA-A Receptor Agonists; D058787:GABA-A Receptor Antagonists; D006993:Hypnotics and Sedatives; D011963:Receptors, GABA-A; C524644:Yi-Gan San; D005442:Flumazenil; D010424:Pentobarbital; D001640:Bicuculline