title: Development and validation of a capillary electrophoresis assay for the determination of the stereoisomeric purity of chloroquine enantiomers.
abstract: A stereoselective CE assay for the determination of the enantiomeric purity of (R)-(-)-chloroquine and (S)-(+)-chloroquine was developed and validated. The separations were performed in a 50.2/40 cm uncoated fused silica capillary at 20°C using a 100 mM sodium phosphate buffer, pH 2.5, containing 30 mg/mL sulfobutylether(VII)-β-cyclodextrin as background electrolyte operated at an applied voltage of -25 kV and 20°C. The detection wavelength was 225 nm. Carbamazepine was used as internal standard. The assay was validated in the range of 0.05-1.0% for the respective minor chloroquine enantiomer based on a concentration of 3 mg/mL of the major enantiomer, either (R)-(-)-chloroquine or (S)-(+)-chloroquine. The method was applied to analyze the stereoisomeric purity of synthetic samples of the chloroquine enantiomers.
authors: Department of Pharmaceutical Chemistry, Friedrich Schiller University Jena, Jena, Germany.
authors: Sudaporn
authors: S
authors: Wongwan
authors: Gerhard K E
authors: GK
authors: Scriba
mesh_terms: D002738:Chloroquine / Q000032:analysis / Q000737:chemistry*; D004340:Drug Contamination; D019075:Electrophoresis, Capillary / Q000379:methods*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D047392:beta-Cyclodextrins; C093196:SBE4-beta-cyclodextrin; D002738:Chloroquine