title: The radiolabeling of proteins by the [18F]AlF method.
abstract: A new ([(18)F]AlF)(2+)-binding ligand that contains 1,4,7-triazacyclononane-1,4-diacetate (NODA) attached to a methyl phenylacetic acid group (MPA) was conjugated to N-(2-aminoethyl)maleimide (EM) to form NODA-MPAEM. The NODA-MPAEM was labeled with ([(18)F]AlF)(2+) at 105°C in 49-82% yield and conjugated at room temperature to an antibody Fab' fragment in 69-80% yield (total time ∼50min) and with retention of immunoreactivity. These data indicate that the rapid and simple [(18)F]AlF-labeling method can be easily adapted for preparing heat-sensitive compounds with (18)F quickly and in high yields.
authors: Immunomedics, Inc., Morris Plains, New Jersey, USA. bmcbride@immunomedics.com
authors: William J
authors: WJ
authors: McBride
authors: Christopher A
authors: CA
authors: D'Souza
authors: Robert M
authors: RM
authors: Sharkey
authors: David M
authors: DM
authors: Goldenberg
mesh_terms: D051033:Apoptosis Inducing Factor / Q000737:chemistry*; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods; D011485:Protein Binding
chemical_list: C494829:AIFM1 protein, human; D051033:Apoptosis Inducing Factor; D005462:Fluorine Radioisotopes; D007140:Immunoglobulin Fab Fragments