title: Land application of tylosin and chlortetracycline swine manure: Impacts to soil nutrients and soil microbial community structure.
abstract: The land application of aged chortetracycle (CTC) and tylosin-containing swine manure was investigated to determine associated impacts to soil microbial respiration, nutrient (phosphorus, ammonium, nitrate) cycling, and soil microbial community structure under laboratory conditions. Two silty clay loam soils common to southeastern South Dakota were used. Aerobic soil respiration results using batch reactors containing a soil-manure mixture showed that interactions between soil, native soil microbial populations, and antimicrobials influenced CO(2) generation. The aged tylosin treatment resulted in the greatest degree of CO(2) inhibition, while the aged CTC treatment was similar to the no-antimicrobial treatment. For soil columns in which manure was applied at a one-time agronomic loading rate, there was no significant difference in soil-P behavior between either aged CTC or tylosin and the no-antimicrobial treatment. For soil-nitrogen (ammonium and nitrate), the aged CTC treatment resulted in rapid ammonium accumulation at the deeper 40cm soil column depth, while nitrate production was minimal. The aged CTC treatment microbial community structure was different than the no-antimicrobial treatment, where amines/amide and carbohydrate chemical guilds utilization profile were low. The aged tylosin treatment also resulted in ammonium accumulation at 40 cm column depth, however nitrate accumulation also occurred concurrently at 10 cm. The microbial community structure for the aged tylosin was also significantly different than the no-antimicrobial treatment, with a higher degree of amines/amides and carbohydrate chemical guild utilization compared to the no-antimicrobial treatment. Study results suggest that land application of CTC and tylosin-containing manure appears to fundamentally change microbial-mediated nitrogen behavior within soil A horizons.
authors: Department of Civil and Environmental Engineering, South Dakota School of Mines and Technology, Rapid City, South Dakota 57701, USA. james.stone@sdsmt.edu
authors: James J
authors: JJ
authors: Stone
authors: Erin K
authors: EK
authors: Dreis
authors: Christopher D
authors: CD
authors: Lupo
authors: Sharon A
authors: SA
authors: Clay
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D002751:Chlortetracycline / Q000032:analysis / Q000494:pharmacology*; D004784:Environmental Monitoring; D008372:Manure / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013552:Swine; D015645:Tylosin / Q000032:analysis / Q000494:pharmacology*
chemical_list: D008372:Manure; D012987:Soil; D010758:Phosphorus; D002244:Carbon; D009584:Nitrogen; D002751:Chlortetracycline; D015645:Tylosin