title: Cytotoxicity of gamma-ray in rat immature hippocampal neurons.
abstract: This in vitro study evaluated the detrimental effect of acute gamma (γ)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0~4 Gy γ-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after γ-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose-dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances significantly blocked γ-irradiation-induced cytotoxicity in immature hippocampal neurons. The results suggest that the caspase-dependent cytotoxicity of γ-rays in immature hippocampal cultured neurons may be caused by oxidative stress.
authors: Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National, University, Gwangju 500-757, Korea.
authors: Miyoung
authors: M
authors: Yang
authors: Myoung-Sub
authors: MS
authors: Song
authors: Sung-Ho
authors: SH
authors: Kim
authors: Jong-Choon
authors: JC
authors: Kim
authors: Joong-Sun
authors: JS
authors: Kim
authors: Taekyun
authors: T
authors: Shin
authors: Changjong
authors: C
authors: Moon
mesh_terms: D004999:Amifostine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism / Q000528:radiation effects; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects*; D007770:L-Lactate Dehydrogenase / Q000528:radiation effects; D009474:Neurons / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects*; D011065:Poly(ADP-ribose) Polymerases / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D002392:Catechin; C045651:epigallocatechin gallate; D007770:L-Lactate Dehydrogenase; D011065:Poly(ADP-ribose) Polymerases; D053148:Caspase 3; D004999:Amifostine