title: ³²P-HPLC analysis of N1-(2-carboxy-2-hydroxyethyl)deoxyadenosine: a DNA adduct of the acrylamide-derived epoxide glycidamide.
abstract: Acrylamide (AA) is produced in many types of food products cooked or processed at high temperature. AA is metabolized to the epoxide glycidamide (GA), which can bind to deoxyguanosine and deoxyadenosine in DNA. The GA-derived N7-guanine and N3-adenine adducts are the only products which so far have been analysed in vivo. Because of previous excellent experience from analysis of adducts to N1-adenine, the aim of our study was to investigate if the N1-adenine adduct of GA could be used as a biomarker of AA exposure. A ³²P-postlabelling method was developed and tested (a) on DNA modified in vitro with GA, (b) on cells treated with GA and (c) on liver DNA from mice treated with AA. The N1-adenine adduct of GA (analysed after conversion to N⁶-GA-deoxyadenosine-5'-monophosphate) was easily detected in DNA reacted with GA and in DNA from cells exposed to GA, but not in DNA from mice treated with AA. The reason for this is currently not clearly understood, but some of the possible contributing factors are discussed. The application of the method in other experimental conditions should be further pursued in order to solve this matter.
authors: Department of Biosciences and Nutrition, Karolinska Institute, Novum, SE-14183 Huddinge, Sweden.
authors: Natalia
authors: N
authors: Kotova
authors: Tina
authors: T
authors: Jurén
authors: Kirsi
authors: K
authors: Myöhänen
authors: Michael
authors: M
authors: Cornelius
authors: Lilianne
authors: L
authors: Abramsson-Zetterberg
authors: Josefin
authors: J
authors: Backman
authors: Ulrike
authors: U
authors: Menzel
authors: Per
authors: P
authors: Rydberg
authors: Leif
authors: L
authors: Kronberg
authors: Kirsi
authors: K
authors: Vähäkangas
authors: Dan
authors: D
authors: Segerbäck
mesh_terms: D020106:Acrylamide / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006224:Cricetinae; D003412:Cricetulus; D018736:DNA Adducts / Q000032:analysis* / Q000378:metabolism; D003839:Deoxyadenosines / Q000032:analysis* / Q000378:metabolism; D004852:Epoxy Compounds / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear; D051379:Mice; D013056:Spectrophotometry, Ultraviolet
chemical_list: D018736:DNA Adducts; D003839:Deoxyadenosines; D004852:Epoxy Compounds; D020106:Acrylamide; C071834:glycidamide