title: New functional ligands for ficolin-3 among lipopolysaccharides of Hafnia alvei.
abstract: Ficolin-1 (M), ficolin-2 (L), ficolin-3 (H) and mannan-binding lectin (MBL) activate the complement system and have opsonic activity. The specificity of ficolin-3 is poorly characterized and currently limited to a few ligands only. We present new specific targets for human ficolin-3, identified among lipopolysaccharides (LPSs, endotoxin) of Hafnia alvei. The interaction was restricted to LPSs of four strains: 23, Polish Collection of Microorganisms (PCM) 1200, PCM 1203 and PCM 1205 and limited to their O-specific polysaccharides (O-specific PSs) composed of different numbers of oligosaccharide (OS) repeating units (RUs). Moreover, these LPS/ficolin-3 complexes activated the lectin pathway of complement in a C4b-deposition assay in a calcium- and magnesium-dependent way. A neoglycoconjugate of the O-specific PS fraction of H. alvei 1200 LPS with bovine serum albumin (BSA) was prepared and used as a tool for the determination of ficolin-3 concentration and activity in serum. To confirm a structure of the O-specific PS 1200 selected for the conjugate preparation, structural analysis was performed on a series of O-specific PSs released by the mild acid hydrolysis of the LPS. The isolated O-specific PSs, showing the different length distributions, were devoid of a major part of the core OS region and had Hep-Kdo disaccharide at a reducing end. The neoglycoconjugate was a highly selective tool for the determination of ficolin-3 concentration and activity in serum (lectin pathway activation in the C4b deposition assay) and was not affected by MBL, ficolin-1 and ficolin-2 or natural antibodies.
authors: Laboratory of Immunobiology of Infections, Institute for Medical Biology, Polish Academy of Sciences, Lodowa 106, Lodz, Poland.
authors: Anna
authors: A
authors: Swierzko
authors: Jolanta
authors: J
authors: Lukasiewicz
authors: Maciej
authors: M
authors: Cedzynski
authors: Anna
authors: A
authors: Maciejewska
authors: Wojciech
authors: W
authors: Jachymek
authors: Tomasz
authors: T
authors: Niedziela
authors: Misao
authors: M
authors: Matsushita
authors: Czeslaw
authors: C
authors: Lugowski
mesh_terms: D000818:Animals; D002417:Cattle; D037582:Complement Pathway, Mannose-Binding Lectin / Q000502:physiology; D004731:Endotoxins / Q000737:chemistry*; D020611:Hafnia alvei; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D008024:Ligands; D008070:Lipopolysaccharides / Q000737:chemistry; D019081:O Antigens / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D000097604:Ficolins
chemical_list: D004731:Endotoxins; D037102:Lectins; D008024:Ligands; D008070:Lipopolysaccharides; D019081:O Antigens; D012710:Serum Albumin, Bovine; D000097604:Ficolins