title: Ionic liquid-based homogeneous liquid-liquid microextraction for the determination of antibiotics in milk by high-performance liquid chromatography.
abstract: Ionic liquid-based homogeneous liquid-liquid microextraction (IL-based HLLME) high-performance liquid chromatography was developed and applied to the extraction, separation and determination of some antibiotics in milk. The proteins and lipids were removed by adding salt and adjusting the pH value. The homogeneous extraction was applied to the improvement of recoveries for IL phase and analytes. The experimental parameters of the IL-based HLLME, including salt concentration in sample solution, pH value of sample solution, volume of [C(6)MIM][BF(4)], amount of ion-pairing agent (NH(4)PF(6)), and extraction time, were evaluated. The limits of detection for enoxacin, pefloxacin, norfloxacin, enrofloxacin, sulfamethoxazole and sulfadimethoxine were 15.8, 7.07, 5.13, 4.00, 7.79 and 8.33 μg L(-1), respectively. When the proposed method was applied to the analysis of milk samples the recoveries of the analytes ranged from 92.5 to 118.6% and relative standard deviations were lower than 7.00%.
authors: College of Chemistry, Jilin University, Changchun, China.
authors: Shiqian
authors: S
authors: Gao
authors: Haiyan
authors: H
authors: Jin
authors: Jingyan
authors: J
authors: You
authors: Yi
authors: Y
authors: Ding
authors: Nan
authors: N
authors: Zhang
authors: Ying
authors: Y
authors: Wang
authors: Ruibing
authors: R
authors: Ren
authors: Rui
authors: R
authors: Zhang
authors: Hanqi
authors: H
authors: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D024841:Fluoroquinolones / Q000032:analysis / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry; D059627:Liquid Phase Microextraction / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D012680:Sensitivity and Specificity; D012965:Sodium Chloride / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D052578:Ionic Liquids; D010756:Phosphoric Acids; D013449:Sulfonamides; D012965:Sodium Chloride; C513217:ammonium hexafluorophosphate