title: Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment.
abstract: There is a need for reliable and sensitive biomarkers for renal impairments to detect early signs of kidney toxicity and to monitor progression of disease. Here, antibody suspension bead arrays were applied to profile plasma samples from patients with four types of kidney disorders: glomerulonephritis, diabetic nephropathy, obstructive uropathy, and analgesic abuse. In total, 200 clinical renal-associated cases and control plasma samples from different cohorts were profiled. Parallel plasma protein profiles were obtained using biotinylated and nonfractionated samples and a selected set of 94 proteins targeted by 129 antigen-purified polyclonal antibodies. Out of the analyzed target proteins, human fibulin-1 was detected at significantly higher levels in the glomerulonephritis patient group compared to the controls and with elevated levels in patient samples for all other renal disorders investigated. Two polyclonal antibodies and one monoclonal antibody directed toward separate, nonoverlapping epitopes showed the same trend in the discovery cohorts. A technical verification using Western blot analysis of selected patient plasma confirmed the trends toward higher abundance of the target protein in disease samples. Furthermore, a verification study was carried out in the context of glomerulonephritis using an independent case and control cohort, and this confirmed the results from the discovery cohort, suggesting that plasma levels of fibulin-1 could serve as a potential indicator to monitor kidney malfunction or kidney damage.
authors: Royal Institute of Technology, Solna, Sweden.
authors: Maja
authors: M
authors: Neiman
authors: Jesper J
authors: JJ
authors: Hedberg
authors: Pierre R
authors: PR
authors: Dönnes
authors: Ina
authors: I
authors: Schuppe-Koistinen
authors: Stephan
authors: S
authors: Hanschke
authors: Ralf
authors: R
authors: Schindler
authors: Mathias
authors: M
authors: Uhlén
authors: Jochen M
authors: JM
authors: Schwenk
authors: Peter
authors: P
authors: Nilsson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods; D012372:ROC Curve; D051437:Renal Insufficiency / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; C060820:fibulin