title: Analysis of chemotaxis in Dictyostelium.
abstract: Dictyostelium discoideum is an excellent model organism for the study of directed cell migration, since Dictyostelium cells show robust chemotactic responses to the chemoattractant cAMP. Many powerful experimental tools are applicable, including forward and reverse genetics, biochemistry, microscopy, and proteomics. Recent studies have demonstrated that many components involved in chemotaxis are functionally conserved between human neutrophils and Dictyostelium amoebae. In this chapter, we describe how to define the functions of proteins that mediate and regulate cell motility, cell polarity, and directional sensing during chemotaxis in Dictyostelium.
authors: Department of Cell Biology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
authors: Huaqing
authors: H
authors: Cai
authors: Chuan-Hsiang
authors: CH
authors: Huang
authors: Peter N
authors: PN
authors: Devreotes
authors: Miho
authors: M
authors: Iijima
mesh_terms: D001681:Biological Assay; D002465:Cell Movement / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004023:Dictyostelium / Q000502:physiology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000242:Cyclic AMP; D051057:Proto-Oncogene Proteins c-akt