title: Periodontal regeneration using a bilayered PLGA/calcium phosphate construct.
abstract: The regeneration of tissues affected by periodontal disease is a complex process; it encompasses the formation of bone, cementum and periodontal ligament. We developed a semi-rigid PLGA (polylactide-co-glycolide acid)/CaP (calcium phosphate) bilayered biomaterial construct to promote periodontal regeneration, which has a continuous outer barrier membrane and an inner topographically complex component. Our experimental model compared periodontal prophylaxis alone with prophylaxis and biomaterial implantation in the treatment of class II furcation defects in dogs. Clinical evaluation, micro-computed tomography, histology and backscattered electron imaging were used for data analysis. Healing occurred uneventfully and bone volumetric values, trabecular number and trabecular thickness were all significantly greater in the treated group; while trabecular separation was significantly greater in the control group. New cementum, bone, and periodontal ligament with Sharpey fibre insertions were only seen in the treated group. Although periodontal regeneration has been reported elsewhere, the advantages of employing our bilayered PLGA + CaP construct are twofold: 1)it did not collapse into the defect; and, 2) its inner side was able to retain the blood clot throughout the buccal defect. The result was greater periodontal regeneration than has previously been reported with traditional flexible membranes.
authors: Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, Minas Gerais, CEP 36570-000, Brazil.
authors: Emily C
authors: EC
authors: Carlo Reis
authors: Andréa P B
authors: AP
authors: Borges
authors: Michel V F
authors: MV
authors: Araújo
authors: Vanessa C
authors: VC
authors: Mendes
authors: Limin
authors: L
authors: Guan
authors: John E
authors: JE
authors: Davies
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D019344:Lactic Acid / Q000494:pharmacology*; D008297:Male; D008855:Microscopy, Electron, Scanning; D010510:Periodontal Diseases / Q000000981:diagnostic imaging / Q000473:pathology; D010519:Periodontium / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D011100:Polyglycolic Acid / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity / Q000187:drug effects; D012038:Regeneration / Q000187:drug effects*; D044968:Regenerative Medicine / Q000379:methods*; D013499:Surface Properties / Q000187:drug effects; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C020243:calcium phosphate