title: Kinetic plot based comparison of the efficiency and peak capacity of high-performance liquid chromatography columns: theoretical background and selected examples.
abstract: The present contribution reviews the foundations of the kinetic-plot method for the direct comparison of the kinetic performance of different chromatographic support and operating modes. The method directly uses experimental data collected for a specific sample and operating condition of one's interest, and is applicable both under isocratic- and gradient-elution conditions. Experimental proof is provided for the strong relation between the kinetic performance of a given support under isocratic and gradient conditions: a material offering superior kinetic performances under isocratic conditions will remain superior under gradient conditions and vice versa provided the comparison occurs under unbiased conditions. In addition, a review is made of the recent literature using the kinetic-plot method to compare and assess the kinetic performance of high performance HPLC columns and their operation mode.
authors: Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium.
authors: Ken
authors: K
authors: Broeckhoven
authors: Deirdre
authors: D
authors: Cabooter
authors: Sebastiaan
authors: S
authors: Eeltink
authors: Gert
authors: G
authors: Desmet
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007700:Kinetics; D008956:Models, Chemical; D016062:Porosity; D011312:Pressure; D013696:Temperature