title: Phenotypic and molecular characterization of human salmonella enterica serovar 4,[5],12:i:- isolates in Slovakia.
abstract: Forty-three epidemiologically unrelated emerging Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strains isolated during the period 2009-2010 in Slovakia were characterized by phenotypic and genotypic methods. Thirty-one isolates (72.1%) expressed resistance to ampicillin, streptomycin, sulfizoxazole, and tetracycline [R-type ASSuT]. The majority of the strains belonged to both definitive phage types DT193 (30.2%) and U311 (27.9%). Other phage types identified were U302 (6.9%), DT18 (4.7%), and DT194 (2.3%). Twelve strains (27.9%) were not typeable. Pulsed-field gel electrophoresis analysis identified three closely related major banding profiles (X1, X1a, and X2), suggesting the similarity and close epidemiological relationship between S. enterica serovar 4,[5],12:i:- strains. In two isolates with R-type ASSuT, phage type NT and in one isolate with R-type ACROSSuSxTTTMPNA, phage type DT193 class 1 integrons were found encoding bla(PSE-1) and dfrA, aadA1, respectively.
authors: Department of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 14, 833 03 Bratislava, Slovakia.
authors: Viktor
authors: V
authors: Majtan
authors: Lubica
authors: L
authors: Majtanova
authors: Juraj
authors: J
authors: Majtan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001434:Bacteriophage Typing; D024881:Drug Resistance, Bacterial; D005759:Gastroenteritis / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D032023:Integrons; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D012480:Salmonella Infections / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents