title: Overexpression of UDP-GlcNAc transporter partially corrects galactosylation defect caused by UDP-Gal transporter mutation.
abstract: Nucleotide sugar transporters deliver substrates for glycosyltransferases into the endoplasmic reticulum and the Golgi apparatus. We demonstrated that overexpression of UDP-GlcNAc transporter (NGT) in MDCK-RCA(r) and CHO-Lec8 mutant cells defective in UDP-Gal transporter (UGT) restored galactosylation of N-glycans. NGT overexpression resulted in decreased transport of UDP-GlcNAc into the Golgi vesicles. This effect resembled the phenotype of mutant cells corrected by UGT1 overexpression. The transport of UDP-Gal was not significantly changed. Our data suggest that the biological function of UGT and NGT in galactosylation of macromolecules may be coupled.
authors: Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland.
authors: Dorota
authors: D
authors: Maszczak-Seneczko
authors: Teresa
authors: T
authors: Olczak
authors: Piotr
authors: P
authors: Jakimowicz
authors: Mariusz
authors: M
authors: Olczak
mesh_terms: D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004285:Dogs; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D011134:Polysaccharides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014531:Uridine Diphosphate Galactose / Q000378:metabolism; D000099237:UDP-Galactose Translocators
chemical_list: D006023:Glycoproteins; D037102:Lectins; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D011134:Polysaccharides; D014531:Uridine Diphosphate Galactose; D000099237:UDP-Galactose Translocators; C121945:UDP-N-acetylglucosamine transporter