title: Clinical use of photodynamic antimicrobial chemotherapy for the treatment of deep carious lesions.
abstract: The purpose of this study was to assess photodynamic antimicrobial chemotherapy (PACT) via irradiation, using a low power laser associated with a photosensitization dye, as an alternative to remove cariogenic microorganisms by drilling. Remaining dentinal samples in deep carious lesions on permanent molars (n = 26) were treated with 0.01% methylene blue dye and irradiated with a low power laser (InGaAIP - indium gallium aluminum phosphide; λ = 660 nm; 100 mW; 320 Jcm(-2); 90 s; 9J). Samples of dentin from the pulpal wall region were collected with a micropunch before and immediately after PACT and kept in a transport medium for microbiological analysis. Samples were cultured in plates of Brucella blood agar, Mitis Salivarius Bacitracin agar and Rogosa SL agar to determine the total viable bacteria, mutans streptococci and Lactobacillus spp. counts, respectively. After incubation, colony-forming units were counted and microbial reduction was calculated for each group of bacteria. PACT led to statistically significant reductions in mutans streptococci (1.38 log), Lactobacillus spp. (0.93 log), and total viable bacteria (0.91 log). This therapy may be an appropriate approach for the treatment of deep carious lesions using minimally invasive procedures.
authors: University of São Paulo, Department of Restorative Dentistry, School of Dentistry, Av Lineu Prestes, 2227-05508-000 São Paulo SP, Brazil. camigugli@usp.br
authors: Camila de Almeida B
authors: Cde A
authors: Guglielmi
authors: Maria Regina L
authors: MR
authors: Simionato
authors: Karen Müller
authors: KM
authors: Ramalho
authors: José Carlos P
authors: JC
authors: Imparato
authors: Sérgio Luiz
authors: SL
authors: Pinheiro
authors: Maria A A C
authors: MA
authors: Luz
mesh_terms: D000293:Adolescent; D000328:Adult; D001419:Bacteria / Q000187:drug effects*; D002648:Child; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D003794:Dental Restoration, Temporary / Q000379:methods*; D003804:Dentin / Q000382:microbiology; D005260:Female; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008751:Methylene Blue / Q000627:therapeutic use; D019060:Minimally Invasive Surgical Procedures; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011859:Radiography; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects; D014070:Tooth / Q000000981:diagnostic imaging / Q000473:pathology
chemical_list: D017319:Photosensitizing Agents; D008751:Methylene Blue