title: The C242T polymorphism of the p22-phox gene (CYBA) is associated with higher left ventricular mass in Brazilian hypertensive patients.
abstract: BACKGROUND
  Reactive oxygen species have been implicated in the physiopathogenesis of hypertensive end-organ damage. This study investigated the impact of the C242T polymorphism of the p22-phox gene (CYBA) on left ventricular structure in Brazilian hypertensive subjects.
  
  
  METHODS
  We cross-sectionally evaluated 561 patients from 2 independent centers [Campinas (n = 441) and Vitória (n = 120)] by clinical history, physical examination, anthropometry, analysis of metabolic and echocardiography parameters as well as p22-phox C242T polymorphism genotyping. In addition, NADPH-oxidase activity was quantified in peripheral mononuclear cells from a subgroup of Campinas sample.
  
  
  RESULTS
  Genotype frequencies in both samples were consistent with the Hardy- Weinberg equilibrium. Subjects with the T allele presented higher left ventricular mass/height2.7 than those carrying the CC genotype in Campinas (76.8 ± 1.6 vs 70.9 ± 1.4 g/m2.7; p = 0.009), and in Vitória (45.6 ± 1.9 vs 39.9 ± 1.4 g/m2.7; p = 0.023) samples. These results were confirmed by stepwise regression analyses adjusted for age, gender, blood pressure, metabolic variables and use of anti-hypertensive medications. In addition, increased NADPH-oxidase activity was detected in peripheral mononuclear cells from T allele carriers compared with CC genotype carriers (p = 0.03).
  
  
  CONCLUSIONS
  The T allele of the p22-phox C242T polymorphism is associated with higher left ventricular mass/height 2.7 and increased NADPH-oxidase activity in Brazilian hypertensive patients. These data suggest that genetic variation within NADPH-oxidase components may modulate left ventricular remodeling in subjects with systemic hypertension.
authors: Department of Internal Medicine, University of Campinas, Campinas, Brazil.
authors: Roberto
authors: R
authors: Schreiber
authors: Maria C
authors: MC
authors: Ferreira-Sae
authors: Juliana A
authors: JA
authors: Ronchi
authors: José A
authors: JA
authors: Pio-Magalhães
authors: José A
authors: JA
authors: Cipolli
authors: José R
authors: JR
authors: Matos-Souza
authors: José G
authors: JG
authors: Mill
authors: Aníbal E
authors: AE
authors: Vercesi
authors: José E
authors: JE
authors: Krieger
authors: Kleber G
authors: KG
authors: Franchini
authors: Alexandre C
authors: AC
authors: Pereira
authors: Wilson
authors: W
authors: Nadruz Junior
mesh_terms: D000483:Alleles; D001938:Brazil; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D006973:Hypertension / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D017379:Hypertrophy, Left Ventricular / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D020257:Ventricular Remodeling / Q000235:genetics / Q000502:physiology
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human