title: Sixty years from discovery to solution: crystal structure of bovine liver catalase form III.
abstract: The crystallization and structural characterization of bovine liver catalase (BLC) has been intensively studied for decades. Forms I and II of BLC have previously been fully characterized using single-crystal X-ray diffraction. Form III has previously been analyzed by electron microscopy, but owing to the thinness of this crystal form an X-ray crystal structure had not been determined. Here, the crystal structure of form III of BLC is presented in space group P2(1)2(1)2(1), with unit-cell parameters a = 68.7, b = 173.7, c = 186.3 Å. The asymmetric unit is composed of the biological tetramer, which is packed in a tetrahedron motif with three other BLC tetramers. This higher resolution structure has allowed an assessment of the previously published electron-microscopy studies.
authors: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
authors: Leila M
authors: LM
authors: Foroughi
authors: You Na
authors: YN
authors: Kang
authors: Adam J
authors: AJ
authors: Matzger
mesh_terms: D000818:Animals; D002374:Catalase / Q000737:chemistry* / Q000648:ultrastructure; D002417:Cattle; D008099:Liver / Q000201:enzymology*; D008854:Microscopy, Electron; D014961:X-Ray Diffraction
chemical_list: D002374:Catalase