title: Wound healing efficacy of Jatyadi Taila: in vivo evaluation in rat using excision wound model.
abstract: BACKGROUND
  In traditional Indian medicinal treatise there are several Ayurvedic formulations mentioned which have been claimed as potential wound healing agents like Madhu Ghrita and Jatyadi Taila. Jatyadi Taila (JT) is a medicated oil formulation (Taila) popularly used in the treatment of various topical wounds.
  
  
  OBJECTIVE
  Though JT has its composition recorded in ancient Ayurvedic texts, there have been minimal attempts to standardize its use in the management of wound. The current work evaluates the wound healing efficacy of JT and also provides evidence of the dermal absorption kinetics of Karanjin from JT.
  
  
  METHODS
  JT was subjected to preliminary phytochemical evaluation. Therapeutically active marker components β-sitosterol, lupeol and karanjin were detected and separated using HPTLC. As a part of safety evaluation, skin irritation potential of JT was evaluated on rabbit skin. Excision wound model in rats were used to evaluate the wound healing efficacy of JT. Histopathological and biochemical evaluations of excised skin tissues at wound sites were carried out. The HPTLC method developed was also validated to evaluate the pharmacokinetics of Karanjin from JT after topical application on pinna of rabbit.
  
  
  RESULTS
  Preliminary phytochemical evaluation of JT revealed presence of flavonoids, essential oils, tannins, glycosides, steroids and alkaloids while resins were found to be absent. HPTLC confirmed the presence of karanjin, lupeol and β-sitosterol in JT. JT was found to be non-irritant when applied to the skin of rabbits. Topical application of JT on excision wounds caused significantly faster reduction in wound area as compared to the application of modern topical formulation (Neosporin(®)) and untreated control wounds. Animals treated with JT showed significant increase in protein, hydroxyproline and hexosamine content in the granulation tissue when compared with the untreated controls. Wound healing potential of JT was found to be dose dependant. HPTLC method was successfully used to evaluate the pharmacokinetics of Karanjin after topical application of JT on rabbit pinna.
  
  
  CONCLUSIONS
  Current work demonstrates a modern approach towards standardization of the use of traditional topical formulation JT. The results justify the traditional claim of JT for its use in the management of wounds.
authors: Herbal Research Lab, Ramnarain Ruia College, Matunga (East), Mumbai 400019, India. sunitashailajan@yahoo.co.in
authors: Sunita
authors: S
authors: Shailajan
authors: Sasikumar
authors: S
authors: Menon
authors: Suhas
authors: S
authors: Pednekar
authors: Ashish
authors: A
authors: Singh
mesh_terms: D000287:Administration, Topical; D000588:Amines / Q000378:metabolism; D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D001578:Benzopyrans / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005260:Female; D006097:Granulation Tissue / Q000187:drug effects* / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008515:Medicine, Ayurvedic; D009355:Neomycin / Q000494:pharmacology; D053978:Pentacyclic Triterpenes / Q000494:pharmacology / Q000627:therapeutic use; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D011112:Polymyxin B / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012855:Sitosterols / Q000494:pharmacology / Q000627:therapeutic use; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism; D000094042:Lupanes
chemical_list: D000588:Amines; D001414:Bacitracin; D001578:Benzopyrans; D004338:Drug Combinations; D006909:Hydroxyproline; D009355:Neomycin; D053978:Pentacyclic Triterpenes; D010936:Plant Extracts; D011112:Polymyxin B; D011506:Proteins; D012855:Sitosterols; C025473:gamma-sitosterol; C001816:bacitracin zinc, neomycin sulfate, polymyxin B, drug combination; C010480:lupeol; D000094042:Lupanes; C052068:karanjin