title: Dinucleotide cap analogue affinity resins for purification of proteins that specifically recognize the 5' end of mRNA.
abstract: Here we present first dinucleotide affinity resins for purification of proteins that specifically recognize the 5' end of mRNA. Constructed resins possess either a naturally occurring mono- or trimethylated cap or their analogues resistant towards enzymatic degradation, bearing a CH(2) bridge between β and γ position of the 5',5'-triphosphate chain. All cap analogues were attached to a polymer support (EAH-Sepharose) through the carboxylic group that had been generated by derivatization of the 2',3'-cis diol of the second nucleotide in the cap structure with levulinic acid.
authors: Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland. marzena@chem.uw.edu.pl
authors: Marzena
authors: M
authors: Jankowska-Anyszka
authors: Karolina
authors: K
authors: Piecyk
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D011485:Protein Binding; D012314:RNA Cap Analogs / Q000737:chemistry / Q000378:metabolism*; D012315:RNA Caps / Q000737:chemistry / Q000378:metabolism*; D012685:Sepharose / Q000737:chemistry
chemical_list: D029742:Caenorhabditis elegans Proteins; D012314:RNA Cap Analogs; D012315:RNA Caps; D012685:Sepharose