title: Multisite haplotype on cattle chromosome 3 is associated with quantitative trait locus effects on lactation traits.
abstract: The goal of this study was to identify candidate genes and DNA polymorphisms for quantitative trait loci (QTL) affecting milk yield (MY), fat yield (FY), and protein yield (PY) previously mapped to bovine chromosome 3 (BTA3). To accomplish this, 373 half-siblings sired by three bulls previously shown to be segregating for lactation trait QTL, and 263 additional sires in the U.S. Dairy Bull DNA Repository (DBDR) were genotyped for 2,500 SNPs within a 16.3 Mbp QTL critical region on BTA3. Targeted resequencing of ∼1.8 Mbp within the QTL critical region of one of the QTL heterozygous sires identified additional polymorphisms useful for association studies. Twenty-three single nucleotide polymorphisms (SNPs) within a fine-mapped region were associated with effects on breeding values for MY, FY, or PY in DBDR sires, of which five SNPs were in strong linkage disequilibrium in the population. This multisite haplotype included SNPs located within exons or promoters of four tightly linked genes: RAP1A, ADORA3, OVGP1, and C3H1orf88. An SNP within RAP1A showed strong evidence of a recent selective sweep based on integrated haplotype score and was also associated with breeding value for PY. Because of its known function in alveolar lumen formation in the mammary gland, RAP1A is thus a strong candidate gene for QTL effects on lactation traits. Our results provide a detailed assessment of a QTL region that will be a useful guide for complex traits analysis in humans and other noninbred species.
authors: Institute for Genomic Biology, Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors: Miri
authors: M
authors: Cohen-Zinder
authors: Ravikiran
authors: R
authors: Donthu
authors: Denis M
authors: DM
authors: Larkin
authors: Charu Gupta
authors: CG
authors: Kumar
authors: Sandra L
authors: SL
authors: Rodriguez-Zas
authors: Kalista E
authors: KE
authors: Andropolis
authors: Rosane
authors: R
authors: Oliveira
authors: Harris A
authors: HA
authors: Lewin
mesh_terms: D000818:Animals; D020029:Base Pairing / Q000235:genetics; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003433:Crosses, Genetic; D005260:Female; D056726:Genetic Association Studies; D006239:Haplotypes / Q000235:genetics*; D006579:Heterozygote; D007774:Lactation / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D008297:Male; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA