title: The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects.
abstract: The correlation of glutathione S-transferase (GST) M1/T1 genetic polymorphisms with oxidative stress-related chronic diseases was proved recently. The aim of the present study was to investigate the association of GSTM1/T1 genetic polymorphisms with antioxidant biomarkers and consumption of fruits and vegetables (F&V) in healthy subjects. In this study, for conducting a 3 d dietary survey, 190 healthy adults were recruited. After DNA extraction, a multiple PCR method was used for GSTM1/T1 genotyping. A spectrophotometer method was applied for the determination of plasma total antioxidant capacity (T-AOC), vitamin C level and erythrocyte GST enzyme activity. A general linear model was used to compare the mean values of antioxidant parameters for different GSTM1/T1 genotypes and consumption of F&V. Polymorphisms of GSTM1/T1 had no effects on plasma T-AOC and vitamin C levels. Deletion of the GSTM1 gene decreased the erythrocyte GST activity. There was correlation between plasma T-AOC and consumption of F&V in the GSTM1⁻ or GSTT1⁺ subjects. A similar pattern was evident for erythrocyte GST activity in the GSTM1⁻ subjects. No association was found among consumption of F&V and GSTM1/T1 genotypes and plasma vitamin C level. Different consumption of F&V had no impact on plasma T-AOC and vitamin C levels in the GSTM1⁻/GSTT1⁺ or GSTM1⁻/GSTT1⁻ subjects. The erythrocyte GST activity was more sensitive to consumption of F&V in the individuals with the GSTM1⁻/GSTT1⁺ genotype. Association was found among GSTM1/T1 genotypes, antioxidant parameters and consumption of F&V. Large-scale and multiple ethnic studies are needed to further evaluate the relationship.
authors: Department of Nutrition and Food Hygiene, School of Public Health and Family Medicine, Capital Medical University, No. 10, Xitoutiao, You An Men, Beijing 100069, People's Republic of China.
authors: Lin-Hong
authors: LH
authors: Yuan
authors: Li-Ping
authors: LP
authors: Meng
authors: Wei-Wei
authors: WW
authors: Ma
authors: Sheng
authors: S
authors: Li
authors: Jin-Fang
authors: JF
authors: Feng
authors: Huan-Ling
authors: HL
authors: Yu
authors: Rong
authors: R
authors: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000032:analysis; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002681:China; D004032:Diet; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005260:Female; D005638:Fruit; D017353:Gene Deletion; D056726:Genetic Association Studies; D005982:Glutathione Transferase / Q000097:blood / Q000235:genetics*; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D014675:Vegetables; D055815:Young Adult
chemical_list: D000975:Antioxidants; D001205:Ascorbic Acid; D015415:Biomarkers; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1