title: Identification of a new protective antigen of Bordetella pertussis.
abstract: Antigenic proteins whose expression is induced under iron starvation, an environmental condition that bacterial pathogens have to face during colonization, might be potential candidates for improved vaccine. By mean of immune proteomics we identified novel antigens of Bordetella pertussis maximally expressed under iron limitation. Among them, Bp1152 (named as IRP1-3) showed a particularly strong reaction with human IgG purified from pooled sera of pertussis-infected individuals. Computer analysis showed IRP1-3 as a dimeric membrane protein potentially involved in iron uptake. Experimental data revealed the surface-exposure of this protein and showed its increase under iron starvation to be independent of bacterial virulence phase. Immunization of mice with the recombinant IRP1-3 resulted in a strong antibody response. These antibodies not only recognized the native protein on bacterial surface but also promote effective bacterial phagocytosis by human PMN, a key protecting activity against this pathogen. Accordingly, IRP1-3 proved protective against B. pertussis infection in mouse model. Expression of IRP1-3 was found conserved among clinical isolates of B. pertussis and positively regulated by iron starvation in these strains. Taken together these results suggest that this protein might be an interesting novel vaccine candidate.
authors: Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
authors: Jimena
authors: J
authors: Alvarez Hayes
authors: Esteban
authors: E
authors: Erben
authors: Yanina
authors: Y
authors: Lamberti
authors: Miguel
authors: M
authors: Ayala
authors: Fabricio
authors: F
authors: Maschi
authors: Cecilia
authors: C
authors: Carbone
authors: Blanca
authors: B
authors: Gatti
authors: Gustavo
authors: G
authors: Parisi
authors: Maria Eugenia
authors: ME
authors: Rodriguez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D005260:Female; D008565:Membrane Proteins / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008565:Membrane Proteins; D010567:Pertussis Vaccine; D011994:Recombinant Proteins