title: Monitoring integrin activation by fluorescence resonance energy transfer.
abstract: Aberrant integrin activation is associated with several immune pathologies. In leukocyte adhesion deficiency (LAD), the absence or inability of β(2) integrins to undergo affinity upregulation contributes to recurrent infectious episodes and impaired wound healing, while excessive integrin activity leads to an exaggerated inflammatory response with associated tissue damage. Therefore, integrin activation is an attractive target for immunotherapies, and monitoring the effect of agents on integrin activation is necessary during preclinical drug development. The activation of integrins involves the structural rearrangement of both the extracellular and cytoplasmic domains. Here, we describe methods for monitoring integrin conformational activation using fluorescence resonance energy transfer (FRET).
authors: Department of Microbiology and Immunology, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester, Rochester, NY, USA.
authors: Craig T
authors: CT
authors: Lefort
authors: Young-Min
authors: YM
authors: Hyun
authors: Minsoo
authors: M
authors: Kim
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D020014:K562 Cells; D009504:Neutrophils / Q000378:metabolism
chemical_list: D016023:Integrins