title: RNA dynamics: perspectives from spin labels.
abstract: Dynamics are important and indispensible physical attributes that play essential roles in RNA function. RNA dynamics are complex, spanning vast timescales, and encompassing a large number of physical modes. The technique of site-directed spin labeling (SDSL), which derives information on local structural and dynamic features of a macromolecule by monitoring a chemically stable nitroxide radical using electron paramagnetic resonance spectroscopy, has been applied to monitor intrinsic dynamics at defined structural states as well as to probe conformational transition dynamics of RNAs. The current state of SDSL studies of RNA dynamics is summarized here. Further development and application of SDSL promise to open up many more opportunities for probing RNA dynamics and connecting dynamics to structure and function.
authors: Department of Chemistry, University of Southern California, Los Angeles, CA, USA.
authors: Phuong
authors: P
authors: Nguyen
authors: Peter Z
authors: PZ
authors: Qin
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D016325:HIV Long Terminal Repeat; D009589:Nitrogen Oxides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D016337:RNA, Catalytic / Q000737:chemistry; D013113:Spin Labels
chemical_list: D009589:Nitrogen Oxides; D016337:RNA, Catalytic; D013113:Spin Labels; C427204:hammerhead ribozyme; D012313:RNA; C039900:nitroxyl