title: Brush and spray: a high-throughput systemic acquired resistance assay suitable for large-scale genetic screening.
abstract: Systemic acquired resistance (SAR) is a defense mechanism induced in the distal parts of plants after primary infection. It confers long-lasting protection against a broad spectrum of microbial pathogens. Lack of high-throughput assays has hampered the forward genetic analysis of SAR. Here, we report the development of an easy and efficient assay for SAR and its application in a forward genetic screen for SAR-deficient mutants in Arabidopsis (Arabidopsis thaliana). Using the new assay for SAR, we identified six flavin-dependent monooxygenase1, four AGD2-like defense response protein1, three salicylic acid induction-deficient2, one phytoalexin deficient4, and one avrPphB-susceptible3 alleles as well as a gain-of-function mutant of CALMODULIN-BINDING TRANSCRIPTION ACTIVATOR3 designated camta3-3D. Like transgenic plants overexpressing CAMTA3, camta3-3D mutant plants exhibit compromised SAR and enhanced susceptibility to virulent pathogens, suggesting that CAMTA3 is a critical regulator of both basal resistance and SAR.
authors: College of Biological Sciences, China Agricultural University, Beijing 100094, People's Republic of China.
authors: Beibei
authors: B
authors: Jing
authors: Shaohua
authors: S
authors: Xu
authors: Mo
authors: M
authors: Xu
authors: Yan
authors: Y
authors: Li
authors: Shuxin
authors: S
authors: Li
authors: Jinmei
authors: J
authors: Ding
authors: Yuelin
authors: Y
authors: Zhang
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D060467:Disease Resistance / Q000187:drug effects / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology; D005820:Genetic Testing / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D009154:Mutation / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology; D044742:Peronospora / Q000187:drug effects; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000382:microbiology; D044224:Pseudomonas syringae / Q000187:drug effects; D020156:Salicylic Acid / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; C011006:jasmonic acid; C036216:ethylene; D020156:Salicylic Acid