title: Differential expression of up-regulated cofilin-1 and down-regulated             cofilin-2 characteristic of pancreatic cancer tissues.
abstract: Pancreatic cancer (PC) is one of the most deadly malignant tumors. The aim             of this study was to identify potential biomarkers for PC. Using two-dimensional             gel electrophoresis and liquid chromatography-tandem mass spectrometry, the proteomic             profiles of pancreatic cancerous and non-cancerous tissues from ten patients with             PC were compared. One of the numerous spots that showed stronger intensity in             cancerous compared to non-cancerous tissues was identified as non-muscle cofilin             (cofilin-1). This up-regulation was validated by Western blot analysis. It is             noteworthy that Western blot analysis showed significantly lower expression of             muscle cofilin (cofilin-2) in pancreatic cancerous tissues compared to non-cancerous             tissues. This is the first time that cofilin isoforms (cofilin-1/2) have been             identified to be differentially expressed in pancreatic cancerous tissues. Therefore,             cofilin isoforms may serve as candidates for clinically useful biomarkers or therapeutic             targets for PC.
authors: Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755-8505, Japan.
authors: Yufeng
authors: Y
authors: Wang
authors: Yasuhiro
authors: Y
authors: Kuramitsu
authors: Tomio
authors: T
authors: Ueno
authors: Nobuaki
authors: N
authors: Suzuki
authors: Shigefumi
authors: S
authors: Yoshino
authors: Norio
authors: N
authors: Iizuka
authors: Xiulian
authors: X
authors: Zhang
authors: Masaaki
authors: M
authors: Oka
authors: Kazuyuki
authors: K
authors: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051338:Cofilin 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051340:Cofilin 2 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C495810:CFL1 protein, human; C495815:CFL2 protein, human; D051338:Cofilin 1; D051340:Cofilin 2; D020033:Protein Isoforms