title: Analysis of total and transferrin-bound iron in human serum for pharmacokinetic studies of iron-sucrose formulations.
abstract: BACKGROUND
  Patients with iron-deficiency anemia benefit from intravenous iron therapies. Development of these pharmaceutical agents requires pharmacokinetic studies monitoring levels of both the administered agent and transferrin-bound iron (TBI). Successful pharmacokinetic methods must discriminate iron species.
  
  
  RESULTS
  Routine colorimetric procedures were used to reliably measure total iron and TBI following iron-sucrose administration. Iron was liberated from iron-sucrose allowing the determination of all circulating iron. Solid-phase sample processing allowed the measurement of TBI. Circulating iron-sucrose could then be calculated as the difference between total iron and TBI.
  
  
  CONCLUSIONS
  A reproducible and robust spectrophotometric method was developed and validated for measuring total iron and TBI in human serum following iron-sucrose therapy.
authors: Research & Development Laboratory, MEDTOX Laboratories, New Brighton, MN 55112, USA.
authors: Melissa M
authors: MM
authors: Goggin
authors: Stephanie D
authors: SD
authors: Gozum
authors: David M
authors: DM
authors: Burrows
authors: An
authors: A
authors: Nguyen
authors: Henry F
authors: HF
authors: Corcoran
authors: Heather
authors: H
authors: Dotzauer
authors: Cheng-Min
authors: CM
authors: Tann
authors: Richard A
authors: RA
authors: Lundberg
authors: Gregory C
authors: GC
authors: Janis
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000188:drug therapy*; D003124:Colorimetry / Q000379:methods; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry*; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid; D006801:Humans; D007501:Iron / Q000008:administration & dosage / Q000097:blood* / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods; D014168:Transferrin / Q000378:metabolism*
chemical_list: D005290:Ferric Compounds; D014168:Transferrin; D007501:Iron; D000077605:Ferric Oxide, Saccharated; D005937:Glucaric Acid