title: Large-scale N-glycoproteome map of rat brain tissue: simultaneous characterization of insoluble and soluble protein fractions.
abstract: The large-scale N-glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM BF(4)) and those in soluble fraction, a total number of 462 non-redundant N-glycoprotein groups, including 316 transmembrane glycoproteins, were successfully identified. Correspondingly, 849 unique N-glycosites were confidently recognized. The data set could provide a support for the further in-depth research of brain N-glycosylation, such as for the discovery of candidate drug targets and biomarkers.
authors: Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
authors: Xiaoqiang
authors: X
authors: Qiao
authors: Dingyin
authors: D
authors: Tao
authors: Yanyan
authors: Y
authors: Qu
authors: Liangliang
authors: L
authors: Sun
authors: Liang
authors: L
authors: Gao
authors: Xiaodan
authors: X
authors: Zhang
authors: Zhen
authors: Z
authors: Liang
authors: Lihua
authors: L
authors: Zhang
authors: Yukui
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006031:Glycosylation; D007093:Imidazoles / Q000737:chemistry; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D012995:Solubility
chemical_list: C419324:1-butyl-3-methylimidazolium tetrafluoroborate; D006023:Glycoproteins; D007093:Imidazoles; D020543:Proteome