title: Three-dimensional biomaterials for the study of human pluripotent stem cells.
abstract: The self-renewal and differentiation of human pluripotent stem cells (hPSCs) have typically been studied in flat, two-dimensional (2D) environments. In this Perspective, we argue that 3D model systems may be needed in addition, as they mimic the natural 3D tissue organization more closely. We survey methods that have used 3D biomaterials for expansion of undifferentiated hPSCs, directed differentiation of hPSCs and transplantation of differentiated hPSCs in vivo.
authors: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors: Thomas P
authors: TP
authors: Kraehenbuehl
authors: Robert
authors: R
authors: Langer
authors: Lino S
authors: LS
authors: Ferreira
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D011956:Receptors, Cell Surface