title: [Monoclonal antibodies to type A, B, E and F botulinum neurotoxins].
abstract: Mouse monoclonal antibodies against the most acutely toxic substances, botulinum neurotoxins (BoNTs) of types A, B, E, and F, was generated and characterized, that recognize their respective toxins in natural toxin complex. Based on these antibodies, we developed sandwich-ELISA for quantitative detection of these toxins. For each respective toxin the detection limit of the assay was: BoNT/A - 0.4 ng/ml, BoNT/B - 0.5 ng/ml; BoNT/E - 0.1 ng/ml; and for BoNT/F - 2.4 ng/ml. The developed assays permitted quantitative identification of the BoNTs in canned meat and vegetables. The BNTA-4.1 and BNTA-9.1 antibodies possessed neutralizing activity against natural complex of the botulinium toxin type A in vivo, both individually and in mixture, the mixture of the antibodies neutralized the higher dose of the toxin. The BNTA-4.1 antibody binds specifically the light chain (the chain with protease activity) of the toxin, whereas BNTA-9.1 interacts with the heavy chain. We believe that the BNTA-4.1 and BNTA-9.1 monoclonal antibodies are prospective candidates for development of humanized therapeutic antibodies for treatment of BoNT/A-caused botulism.
authors: S G
authors: SG
authors: Abbasova
authors: N V
authors: NV
authors: Ruddenko
authors: A Iu
authors: AIu
authors: Gorokhovatskiĭ
authors: M V
authors: MV
authors: Kapralova
authors: I D
authors: ID
authors: Vinogradova
authors: Iu V
authors: IuV
authors: Vertiev
authors: V A
authors: VA
authors: Nesmeianov
authors: E V
authors: EV
authors: Grishin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000276:immunology*; D001905:Botulinum Toxins / Q000032:analysis* / Q000276:immunology; D019274:Botulinum Toxins, Type A / Q000032:analysis* / Q000276:immunology; D001906:Botulism / Q000175:diagnosis; D003014:Clostridium botulinum / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D057141:Food, Preserved / Q000032:analysis / Q000382:microbiology; D008460:Meat / Q000032:analysis / Q000382:microbiology; D051379:Mice; D012680:Sensitivity and Specificity; D014675:Vegetables / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; C096323:rimabotulinumtoxinB; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; C096326:botulinum toxin type E; C096321:botulinum toxin type F