title: Maintenance of human pluripotent stem cells using 4SP-hFGF2-secreting STO cells.
abstract: Human embryonic stem cells (hESCs) are typically cultured on fibroblast feeder cells or in fibroblast conditioned medium supplemented with fibroblast growth factor 2 (FGF2, also known as bFGF). FGF signaling appears to be important for hESC self-renewal and is required to enable the culture of hESCs in an undifferentiated state. In this study, we generated a transgenic fibroblast feeder line stably expressing a secretable FGF4 signal peptide tagged hFGF2 (4SP-hFGF2). The expression of this transgene functionally replaced the requirement for exogenous FGF2 when using these cells as feeders for the maintenance of hESCs. Under these conditions, hESCs maintained the typical marker of pluripotency assessed after long term culture, while still retaining the capacity for differentiation to all three germ layers. This transgene could be applied to mass produce 4SP-hFGF2 protein, serving to be an economical and effective strategy for culturing pluripotent stem cells as feeder cells.
authors: CHA Bio & Diostech Co.,Ltd. 606-16 Yeoksam 1 dong, Gangnam gu, Seoul 135-907, Republic of Korea.
authors: Won-Young
authors: WY
authors: Lee
authors: Jumi
authors: J
authors: Kim
authors: Chang-Hyun
authors: CH
authors: Gil
authors: Jae-Ho
authors: JH
authors: Lee
authors: Hyuk
authors: H
authors: Song
authors: Jae-Hwan
authors: JH
authors: Kim
authors: Hyung-Min
authors: HM
authors: Chung
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D001483:Base Sequence; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000378:metabolism*; D051519:Fibroblast Growth Factor 4 / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D005855:Germ Layers / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D019138:Antibodies, Blocking; C496445:FGF4 protein, human; D051519:Fibroblast Growth Factor 4; D007074:Immunoglobulin G; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D016222:Fibroblast Growth Factor 2