title: Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4.
abstract: Galactokinases (GALK) have attracted significant research attention for their potential application in the enzymatic synthesis of unique sugar phosphates. The galactokinase (GalKSpe4) cloned from Streptococcus pneumoniae TIGR4 had a temperature optimum of 45°C, and a pH optimum of 8.0. The substrate specificity and kinetics studies revealed that GalKSpe4 had moderate activity toward glucose, in contrast with very low or no activity observed in other previously reported GALKs. Most interestingly, GalKSpe4 exhibited activity for GalNAc, which had never been recorded in other GALKs found by now. This is the first time to report that bacterial GALK can recognize GalNAc.
authors: The State Key laboratory of Microbial Technology, Shandong University, 27# Shanda South Road, Jinan, Shandong 250100, China.
authors: Min
authors: M
authors: Chen
authors: Lei-lei
authors: LL
authors: Chen
authors: Yang
authors: Y
authors: Zou
authors: Mengyang
authors: M
authors: Xue
authors: Min
authors: M
authors: Liang
authors: Lan
authors: L
authors: Jin
authors: Wan-yi
authors: WY
authors: Guan
authors: Jie
authors: J
authors: Shen
authors: Wenjun
authors: W
authors: Wang
authors: Lei
authors: L
authors: Wang
authors: Jun
authors: J
authors: Liu
authors: Peng George
authors: PG
authors: Wang
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry*; D000595:Amino Acid Sequence; D057075:Enzyme Assays; D005686:Galactokinase / Q000096:biosynthesis / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D013296:Streptococcus pneumoniae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; D005686:Galactokinase; D005947:Glucose; D000116:Acetylgalactosamine