title: Baeyer-Villiger monooxygenases in aroma compound synthesis.
abstract: Baeyer-Villiger monooxygenases (BVMOs) are presented as highly selective and efficient biocatalysts for the synthesis of aroma lactones via kinetic resolution of 2-substituted cycloketones, exemplified with two δ-valerolactones, the jasmine lactones and their ε-caprolactone homologs. Analytical scale screens of our BVMO library ensued by preparative whole-cell biotransformations led to the identification of two enzymes (cyclohexanone monooxygenase from Arthrobacter BP2 and cyclododecanone monooxygenase from Rhodococcus SC1) perfectly suited for the task at hand: easily accessible racemic starting materials were bio-oxidized to almost enantiopure ketones and lactones in good yields (48-74%) and optical purities (ee 93% to >99%, E>100).
authors: Institute for Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9/163, A-1090, Vienna, Austria.
authors: Michael J
authors: MJ
authors: Fink
authors: Florian
authors: F
authors: Rudroff
authors: Marko D
authors: MD
authors: Mihovilovic
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D001711:Biotransformation; D007783:Lactones / Q000737:chemistry / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010476:Perfume / Q000737:chemistry / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology*
chemical_list: D007783:Lactones; D010476:Perfume; D006899:Mixed Function Oxygenases