title: Proteomics and posttranslational proteomics of seed dormancy and germination.
abstract: The seed is the dispersal unit of plants and must survive the vagaries of the environment. It is the object of intense genetic and genomic studies because processes related to seed quality affect crop yield and the seed itself provides food for humans and animals. Presently, the general aim of postgenomics analyses is to understand the complex biochemical and molecular processes underlying seed quality, longevity, dormancy, and vigor. Due to advances in functional genomics, the recent past years have seen a tremendous progress in our understanding of several aspects of seed development and germination. Here, we describe the proteomics protocols (from protein extraction to mass spectrometry) that can be used to investigate several aspects of seed physiology, including germination and its hormonal regulation, dormancy release, and seed longevity. These techniques can be applied to the study of both model plants (such as Arabidopsis) and crops.
authors: AgroParisTech, Unité de Formation et de Recherche de Physiologie Végétale, Paris, France. loic.rajjou@agroparistech.fr
authors: Loïc
authors: L
authors: Rajjou
authors: Maya
authors: M
authors: Belghazi
authors: Julie
authors: J
authors: Catusse
authors: Laurent
authors: L
authors: Ogé
authors: Erwann
authors: E
authors: Arc
authors: Béatrice
authors: B
authors: Godin
authors: Kamel
authors: K
authors: Chibani
authors: Sonia
authors: S
authors: Ali-Rachidi
authors: Boris
authors: B
authors: Collet
authors: Philippe
authors: P
authors: Grappin
authors: Marc
authors: M
authors: Jullien
authors: Karine
authors: K
authors: Gallardo
authors: Claudette
authors: C
authors: Job
authors: Dominique
authors: D
authors: Job
mesh_terms: D000040:Abscisic Acid / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics* / Q000502:physiology; D013058:Mass Spectrometry; D057445:Plant Dormancy / Q000235:genetics* / Q000502:physiology; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D040901:Proteomics; D012639:Seeds / Q000235:genetics / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D000040:Abscisic Acid