title: Bacterial surface display of a co-factor containing enzyme, ω-transaminase from Vibrio fluvialis using the Bacillus subtilis spore display system.
abstract: To improve the conventional bacterial surface display systems and to display a co-factor containing enzyme, ω-transaminase from Vibrio fluvialis, which needs pyridoxal phosphate (PLP) for efficient transamination, Bacillus subtilis spore display system with cotG, as an anchoring motif was used. Flow cytometry of the B. subtilis spore-expressing ω-transaminase proved its surface localization on the spore. The enzymatic activity of the spore expressing ω-transaminase was more than 30 times higher than that of the host spore. Protease treatment of the ω-transaminase displaying spores resulted in decreased transaminase activity, which is in keeping with the surface location of the fusion protein, CotG-ω-transaminase.
authors: Department of Chemical Engineering and Bioengineering and The Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720-3220, USA.
authors: Bum-Yeol
authors: BY
authors: Hwang
authors: Byung-Gee
authors: BG
authors: Kim
authors: June-Hyung
authors: JH
authors: Kim
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001681:Biological Assay; D019281:Dimerization; D005434:Flow Cytometry; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013171:Spores, Bacterial / Q000201:enzymology / Q000235:genetics*; D000637:Transaminases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014733:Vibrio / Q000737:chemistry / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins; C091352:cotG protein, Bacillus subtilis; D011732:Pyridoxal Phosphate; D000637:Transaminases; C019798:branched-chain-amino-acid transaminase