title: High-efficiency upconversion luminescent sensing and bioimaging of Hg(II) by chromophoric ruthenium complex-assembled nanophosphors.
abstract: A chromophoric ruthenium complex-assembled nanophosphor (N719-UCNPs) was achieved as a highly selective water-soluble probe for upconversion luminescence sensing and bioimaging of intracellular mercury ions. The prepared nanophosphors were characterized by X-ray powder diffraction (XRD), transmission electron microscopy (TEM), energy-dispersive X-ray analysis (EDXA), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). Further application of N719-UCNPs in sensing Hg(2+) was confirmed by optical titration experiment and upconversion luminescence live cell imaging. Using the ratiometric upconversion luminescence as a detection signal, the detection limit of Hg(2+) for this nanoprobe in water was down to 1.95 ppb, lower than the maximum level (2 ppb) of Hg(2+) in drinking water set by the United States EPA. Importantly, the nanoprobe N719-UCNPs has been shown to be capable of monitoring changes in the distribution of Hg(2+) in living cells by upconversion luminescence bioimaging.
authors: Department of Chemistry, Fudan University, Shanghai 200433, People's Republic of China.
authors: Qian
authors: Q
authors: Liu
authors: Juanjuan
authors: J
authors: Peng
authors: Lining
authors: L
authors: Sun
authors: Fuyou
authors: F
authors: Li
mesh_terms: D002470:Cell Survival; D003116:Color; D006367:HeLa Cells; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000379:methods*; D008628:Mercury / Q000737:chemistry / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D049408:Luminescent Agents; D009942:Organometallic Compounds; D014867:Water; D012428:Ruthenium; D008628:Mercury