title: Electrochemical determination of hydrogen peroxide with cytochrome c peroxidase and horse heart cytochrome c entrapped in a gelatin hydrogel.
abstract: A novel and versatile method, based on a membrane-free enzyme electrode in which both the enzyme and a mediator protein are entrapped in a gelatine hydrogel was developed for the fabrication of biosensors. As a proof of principle, we prepared a hydrogen peroxide biosensor by successfully entrapping both horse heart cytochrome c (HHC) and Saccharomyces cerevisae cytochrome c peroxidase (CCP) in a gelatin matrix which is immobilized on a gold electrode. This electrode was first pretreated with 6-mercaptohexanol. The biosensor displayed a rapid response and an expanded linear response range from 0 to 0.3 mM (R = 0.987) with a detection limit of 1 × 10(-5)M in a HEPES buffer solution (pH 7.0). This method of encapsulation is now further investigated for industrial biosensor applications.
authors: Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium. karolien.dewael@ua.ac.be
authors: Karolien
authors: K
authors: De Wael
authors: Qamar
authors: Q
authors: Bashir
authors: Sandra
authors: S
authors: Van Vlierberghe
authors: Peter
authors: P
authors: Dubruel
authors: Hendrik A
authors: HA
authors: Heering
authors: Annemie
authors: A
authors: Adriaens
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D003578:Cytochrome-c Peroxidase / Q000737:chemistry / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D000441:Hexanols; D006736:Horses; D006861:Hydrogen Peroxide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D011199:Potentiometry / Q000379:methods*; D012441:Saccharomyces cerevisiae; D013438:Sulfhydryl Compounds
chemical_list: C503488:6-mercapto-1-hexanol; D004800:Enzymes, Immobilized; D000441:Hexanols; D013438:Sulfhydryl Compounds; D006046:Gold; D005780:Gelatin; D045304:Cytochromes c; D006861:Hydrogen Peroxide; D003578:Cytochrome-c Peroxidase