title: Endothelial activation and injury by cigarette smoke exposure.
abstract: Endothelial activation/injury following exposure to cigarette smoke may explain incidence of atherosclerosis and cardiovascular disease in smokers. We investigated cigarette smoke extract (CSE) effects relative to activation, injury, and survival of human umbilical vein endothelial cells (HUVEC) and compared circulating levels of specific endothelial activation markers between smokers and healthy non-smokers before and after smoking cessation. Viability and toxicity of HUVEC were tested by MTT and LDH assay. Release (by endothelial cells) and circulating levels (in smokers) of von Willebrand Factor (vWF), thrombomodulin (TM), was evaluated by ELISA. Incubation with increasing concentrations of CSE reduced the percentage of viable cells, being 33.9%, 23.9% after CSE 4%, 6% respectively. Dose- and time-dependent release of LDH was observed after incubation with CSE. vWF, TM release were assayed after CSE 2% HUVEC stimulation. Significant 42%, 61%, 76% increase in vWF concentration was detected respectively at 30', 60', 120'. Reduction in circulating levels of vWF, from a median value of 144.0% to 123.7%, was observed in the quitters group after smoking cessation. Exposure to cigarette smoke is cytotoxic and induces activation/injury of endothelium in vitro and in vivo. These findings may provide pathogenetic basis by which smoking can predispose to development of atherothrombosis and cardiovascular disease.
authors: Centre for the Prevention and Cure of Tabagism, CPCT, Catania University.
authors: F
authors: F
authors: Guarino
authors: G
authors: G
authors: Cantarella
authors: M
authors: M
authors: Caruso
authors: C
authors: C
authors: Russo
authors: S
authors: S
authors: Mancuso
authors: G
authors: G
authors: Arcidiacono
authors: R R
authors: RR
authors: Cacciola
authors: R
authors: R
authors: Bernardini
authors: R
authors: R
authors: Polosa
mesh_terms: D000328:Adult; D002318:Cardiovascular Diseases / Q000097:blood / Q000209:etiology / Q000517:prevention & control; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000009:adverse effects / Q000737:chemistry*; D003430:Cross-Sectional Studies; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D011446:Prospective Studies; D012907:Smoking / Q000009:adverse effects / Q000097:blood*; D016540:Smoking Cessation; D018180:Thrombomodulin / Q000097:blood; D014026:Nicotiana / Q000009:adverse effects / Q000737:chemistry*; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D045424:Complex Mixtures; D018180:Thrombomodulin; D014841:von Willebrand Factor; D007770:L-Lactate Dehydrogenase