title: The StepOne real-time polymerase chain reaction detection of Salmonella sp., Salmonella enterica ser. typhimurium and enteritidis in milk and meat.
abstract: The aim of this study was to follow contamination of ready to eat milk and meat products with Salmonella spp. by using the StepOne real-time polymerase chain reaction (PCR). Classical microbiological methods for detection of foodborne bacteria involve the use of pre-enrichment and/or specific enrichment, following isolation of bacteria in solid media and the final confirmation by biochemical and/or serological tests. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Salmonella spp. Detection Kit for pursuance of the real-time PCR (Applied Biosystems). In samples without incubation we detected strain of Salmonella sp. in 5 out of 25 samples (swabs), as well as in the internal positive control (IPC), which was positive in all samples. This StepOne real-time PCR assay is extremely useful for any laboratory equipped by real-time PCR. It is a fast, reproducible, simple, specific and sensitive way to detect nucleic acids, which could be used in clinical diagnostic tests in the future. Our results indicated that real-time PCR assay developed in this study could sensitively detect Salmonella spp. in ready-to-eat food. This could prevent infection caused by Salmonella, and also could benefit food manufacturing companies by extending their product's shelf-life as well as saving the cost of warehousing their food products while awaiting pathogen testing results.
authors: Department of Microbiology, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.
authors: Jaroslav
authors: J
authors: Pochop
authors: Miroslava
authors: M
authors: Kačániová
authors: Lukáš
authors: L
authors: Hleba
authors: Jadža
authors: J
authors: Lejková
authors: Martina
authors: M
authors: Fikselová
authors: Simona
authors: S
authors: Kunová
authors: Maciej
authors: M
authors: Kluz
mesh_terms: D000818:Animals; D003611:Dairy Products / Q000382:microbiology*; D005504:Food Analysis; D005506:Food Contamination / Q000032:analysis; D008461:Meat Products / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*