title: Effects of D-cycloserine and midazolam on the expression of the GABA-A alpha-2 receptor subunits in brain structures of fear conditioned rats.
abstract: The role of the GABA-A alpha-2 receptor subunit in the basolateral amygdala (BLA), dentate gyrus of the hippocampus (DG) and prefrontal cortex (M2 area) during a fear session (performed one week after the conditioned fear test), was studied. We employed a model of high (HR) and low anxiety (LR) rats divided according to their conditioned freezing response. Pretreatment of rats with d-cycloserine immediately before the fear session attenuated fear response in HR and LR rats and increased the density of alpha-2 subunits in the BLA, M2 area and DG of HR animals. The less potent behavioural influence of midazolam (in HR group only) was linked to the increased expression of alpha-2 subunit in M2 area and DG. These results support a role of the GABA-A receptor alpha-2 subunit in processing of emotional cortico-hippocampal input to the BLA.
authors: Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 26/28 Krakowskie Przedmieście Street, 00-927 Warsaw, Poland. awislow@yahoo.com
authors: Aleksandra
authors: A
authors: Wisłowska-Stanek
authors: Małgorzata
authors: M
authors: Lehner
authors: Anna
authors: A
authors: Skórzewska
authors: Piotr
authors: P
authors: Maciejak
authors: Janusz
authors: J
authors: Szyndler
authors: Danuta
authors: D
authors: Turzyńska
authors: Alicja
authors: A
authors: Sobolewska
authors: Adam
authors: A
authors: Płaźnik
mesh_terms: D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects*; D003523:Cycloserine / Q000494:pharmacology*; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism*; D005239:Fear / Q000187:drug effects*; D008297:Male; D008874:Midazolam / Q000494:pharmacology*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000096:biosynthesis*
chemical_list: C520011:Gabra2 protein, rat; D011963:Receptors, GABA-A; D003523:Cycloserine; D008874:Midazolam