title: FinTRIMs, fish virus-inducible proteins with E3 ubiquitin ligase activity.
abstract: TRIM proteins have recently emerged as novel players in antiviral defense. TRIM proteins contain a tri-partite motif, composed of a RING zinc finger, one or two B-boxes and a coiled-coil domain. Many members of this large protein family of E3 ubiquitin ligases catalyze the attachment of ubiquitin to a substrate protein, an activity dependent on the RING domain. We earlier made a full description of the TRIM gene family in zebrafish and pufferfish and identified three multigene TRIM subsets, a feature unique to fish. To determine their biological role, we further characterized members of the finTRIM subset. FinTRIM gene expression was studied during development and in multiple tissues in adult rainbow trout. Upregulation of a large number of finTRIM upon viral stimulation suggests they are involved in antiviral immunity. We also demonstrate that two finTRIM members display E3 ubiquitin ligase activity, indicating that finTRIMs could regulate antiviral signaling through ubiquitination.
authors: Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors: Lieke M
authors: LM
authors: van der Aa
authors: Luc
authors: L
authors: Jouneau
authors: Emmanuel
authors: E
authors: Laplantine
authors: Olivier
authors: O
authors: Bouchez
authors: Lidy
authors: L
authors: Van Kemenade
authors: Pierre
authors: P
authors: Boudinot
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D016000:Cluster Analysis; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007150:Immunohistochemistry / Q000662:veterinary; D029141:Novirhabdovirus / Q000276:immunology / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D029941:Fish Proteins; D012313:RNA; D044767:Ubiquitin-Protein Ligases