title: Nucleotide exchange factors for Hsp70 chaperones.
abstract: The ATPase cycle of Hsp70 chaperones controls their transient association with substrate and, thus, governs their function in protein folding. Nucleotide exchange factors (NEFs) accelerate ADP release from Hsp70 which results in rebinding of ATP and release of the substrate. This chapter describes several methods suitable to study NEFs of Hsp70 chaperones. On the one hand, steady-state ATPase assays provide information on how the NEF influences progression of the Hsp70 through the entire ATPase cycle. On the other hand, nucleotide release can be measured directly using labeled nucleotides, which enables identification and further characterization of NEFs.
authors: Zentrum für Molekulare Biologie, der Universität Heidelberg, DKFZ-ZMBH Alliance, Heidelberg, Germany.
authors: Heike
authors: H
authors: Rampelt
authors: Matthias P
authors: MP
authors: Mayer
authors: Bernd
authors: B
authors: Bukau
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009711:Nucleotides / Q000032:analysis / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D009711:Nucleotides; D015513:Oncogene Proteins; D029701:Saccharomyces cerevisiae Proteins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases