title: Interaction of Escherichia coli RNA polymerase with artificial promoters, containing nonnucleotide spacers.
abstract: To study the functional role of the spacer region between two consensus -10 and -35 elements of promoters, recognized by E. coli RNA polymerase, the model promoter-like DNA duplexes containing nonnucleotide inserts (mimicking 17-mer spacer) either in one or both strands, were constructed. The modified duplexes can form the heparin-resistant binary complexes with RNA polymerase. The DNA duplex with nonnucleotide insert in the template strand can specifically direct the synthesis of mRNA in the in vitro run-off transcription assays.
authors: Chemical Department, M. V. Lomonosov Moscow State University, 119992, Moscow, Russian Federation. koroleva@genebee.msu.su
authors: Olga Nikolaevna
authors: ON
authors: Koroleva
authors: Eugeniy Mikhailovich
authors: EM
authors: Volkov
authors: Valeriy Lvovich
authors: VL
authors: Drutsa
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: D009841:Oligonucleotides; D004247:DNA; D012321:DNA-Directed RNA Polymerases