title: Optimization of pretreatment and saccharification for the production of bioethanol from water hyacinth by Saccharomyces cerevisiae.
abstract: Alkaline-oxidative (A/O) pretreatment and enzymatic saccharification were optimized for bioethanol fermentation from water hyacinth by Saccharomyces cerevisiae. Water hyacinth was subjected to A/O pretreatment at various NaOH and H(2)O(2) concentrations and reaction temperatures for the optimization of bioethanol fermentation by S. cerevisiae. The most effective condition for A/O pretreatment was 7% (w/v) NaOH at 100 °C and 2% (w/v) H(2)O(2). The carbohydrate content was analyzed after reaction at various enzyme concentrations and enzyme ratios using Celluclast 1.5 L and Viscozyme L to determine the effective conditions for enzymatic saccharification. After ethanol fermentation using S. cerevisiae KCTC 7928, the concentration of glucose, ethanol and glycerol was analyzed by HPLC using a RI detector. The yield of ethanol in batch fermentation was 0.35 g ethanol/g biomass. Continuous fermentation was carried out at a dilution rate of 0.11 (per h) and the ethanol productivity was 0.77 [g/(l h)].
authors: Department of Biological Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon, 402-751, Korea.
authors: Deuk Joo
authors: DJ
authors: Ahn
authors: Se Kyung
authors: SK
authors: Kim
authors: Hyun Shik
authors: HS
authors: Yun
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors / Q000382:microbiology*; D050260:Carbohydrate Metabolism / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D031806:Eichhornia / Q000737:chemistry* / Q000382:microbiology*; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D056804:Biofuels; D010936:Plant Extracts; D000431:Ethanol