title: Reversal of serologic, immunologic, and histologic dysfunction in mice with systemic lupus erythematosus by long-term serial adipose tissue-derived mesenchymal stem cell transplantation.
abstract: OBJECTIVE
  To investigate the efficacy of human adipose tissue-derived mesenchymal stem cell (AD-MSC) transplantation in systemic lupus erythematosus (SLE) and to determine the optimal transplantation window for stem cells either before or after disease onset.
  
  
  METHODS
  (NZB×NZW)F1 mice with SLE were administered human AD-MSCs (5×10(5)) intravenously every 2 weeks from age 6 weeks until age 60 weeks, while the control group received saline vehicle on the same schedule. Another experiment was carried out with a different initiation time point for serial transplantation (age 6 weeks or age 32 weeks).
  
  
  RESULTS
  Long-term serial administration (total of 28 times) of human AD-MSCs ameliorated SLE without any adverse effects. Compared with the control group, the human AD-MSC-treated group had a significantly higher survival rate with improvement of histologic and serologic abnormalities and immunologic function, and also had a decreased incidence of proteinuria. Anti-double-stranded DNA antibodies and blood urea nitrogen levels decreased significantly with transplantation of human AD-MSCs, and serum levels of granulocyte-macrophage colony-stimulating factor, interleukin-4 (IL-4), and IL-10 increased significantly. A significant increase in the proportion of CD4+FoxP3+ cells and a marked restoration of capacity for cytokine production were observed in spleens from the human AD-MSC-treated group. In the second experiment, an early stage treatment group showed better results (higher survival rates and lower incidence of proteinuria) than an advanced stage treatment group.
  
  
  CONCLUSIONS
  Serial human AD-MSC transplantation had beneficial effects in the treatment of SLE, without adverse effects. Transplantation of human AD-MSCs before disease onset was preferable for amelioration of SLE and restoration of immune homeostasis.
authors: Laboratory Animal Research Center, Samsung Biomedical Research Institute, Seoul, Republic of Korea. vet.cew@gmail.com
authors: Eun Wha
authors: EW
authors: Choi
authors: Il Seob
authors: IS
authors: Shin
authors: So Young
authors: SY
authors: Park
authors: Ji Hyun
authors: JH
authors: Park
authors: Jong Sung
authors: JS
authors: Kim
authors: Eun Ji
authors: EJ
authors: Yoon
authors: Sung Keun
authors: SK
authors: Kang
authors: Jeong Chan
authors: JC
authors: Ra
authors: Sung Hwa
authors: SH
authors: Hong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology / Q000378:metabolism; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006801:Humans; D008136:Longevity; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D008181:Lupus Nephritis / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D009929:Organ Size; D011507:Proteinuria / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D000974:Antibodies, Antinuclear; D016207:Cytokines