title: In vivo ¹H-NMR microimaging during seed imbibition, germination, and early growth.
abstract: Magnetic resonance imaging (MRI) is a superior noninvasive diagnostic tool widely used in clinical medicine, with more than 60 million MRI tests performed each year worldwide. More specialized high-resolution MRI systems capable of a resolution that is 100-1,000 times higher than standard MRI instruments are used primarily in materials science, but are used with increasing frequency in plant physiology. We have shown that high-resolution (1)H-nuclear magnetic resonance (NMR) microimaging can provide a wealth of information about the internal anatomy of plant seeds as small as 1 mm or even smaller. This chapter covers the methods associated with these imaging techniques in detail. We also discuss the application of (1)H-NMR microimaging to study in vivo seed imbibition, germination, and early seedling growth.
authors: Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, ON, Canada.
authors: Victor
authors: V
authors: Terskikh
authors: Kerstin
authors: K
authors: Müller
authors: Allison R
authors: AR
authors: Kermode
authors: Gerhard
authors: G
authors: Leubner-Metzger
mesh_terms: D018525:Germination / Q000502:physiology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D057445:Plant Dormancy / Q000502:physiology; D036226:Seedlings / Q000254:growth & development / Q000502:physiology; D012639:Seeds / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D014867:Water / Q000502:physiology
chemical_list: D014867:Water