title: Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants.
abstract: The antiviral effect of the acyclic nucleoside phosphonate tenofovir (R)-PMPA on double-stranded DNA Cauliflower mosaic virus (CaMV) in Brassica pekinensis plants grown in vitro on liquid medium was evaluated. Double antibody sandwich ELISA and PCR were used for relative quantification of viral protein and detecting nucleic acid in plants. (R)-PMPA at concentrations of 25 and 50 mg/l significantly reduced CaMV titers in plants within 6-9 weeks to levels detectable neither by ELISA nor by PCR. Virus-free plants were obtained after 3-month cultivation of meristem tips on semisolid medium containing 50 mg/l (R)-PMPA and their regeneration to whole plants in the greenhouse. Studying the metabolism of (R)-PMPA in B. pekinensis revealed that mono- and diphosphate, structural analogs of NDP and/or NTP, are the only metabolites formed. The data indicate very low substrate activity of the enzymes toward (R)-PMPA as substrate. The extent of phosphorylation in the plant's leaves represents only 4.5% of applied labeled (R)-PMPA. In roots, we detected no radioactive peaks of phosphorylated metabolites of (R)-PMPAp or (R)-PMPApp.
authors: Biology Centre, v.v.i., Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, 37005 České Budějovice, Czech Republic. spak@umbr.cas.cz
authors: Josef
authors: J
authors: Spak
authors: Ivan
authors: I
authors: Votruba
authors: Daniela
authors: D
authors: Pavingerová
authors: Antonín
authors: A
authors: Holý
authors: Vlastimila
authors: V
authors: Spaková
authors: Karel
authors: K
authors: Petrzik
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000998:Antiviral Agents / Q000378:metabolism* / Q000494:pharmacology*; D001711:Biotransformation; D001937:Brassica / Q000378:metabolism* / Q000821:virology*; D017796:Caulimovirus / Q000187:drug effects* / Q000254:growth & development; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D063065:Organophosphonates / Q000378:metabolism* / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D000068698:Tenofovir; D019562:Viral Load; D014764:Viral Proteins / Q000032:analysis
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D063065:Organophosphonates; D014764:Viral Proteins; D000068698:Tenofovir; D000225:Adenine