title: Profile of NF-κBp(65/NFκBp50) among prostate specific antigen sera levels in prostatic pathologies.
abstract: OBJECTIVE
  The aim of this work was to characterise the immunoexpression of NF-κB (p50/p65) in human prostatic pathologies and to study its profiles of activation among sera prostate specific antigen antigen (PSA) according the three groups: 0-4ng/mL, 4-20ng/mL and >20ng/mL.
  
  
  METHODS
  Twenty-four men with benign prostate hyperplasia (BPH); 19 men with prostate cancer (PC) and five men with normal prostates (NP). Immunohistochemical and western blot analysis was performed. Serum levels of PSA were assayed by immulite autoanalyser.
  
  
  RESULTS
  In BPH and PC samples, immunoexpressions were observed for NF-κBp65 and NF-κBp50; while in NP samples, only were detected NF-κBp50. PC samples showed immunoreactions to NF-κBp65 and NF-κBp50 more intense (respectively 24.18±0.67 and 28.23±2.01) than that observed in BPH samples (respectively18.46±2.04 and 18.66±1.59) with special localisation in the nucleus. Different profiles of NF-κBp65 immunoexpressions were observed and BPH patients with sera PSA levels between 0-4ng/mL presented a significant weak percentage compared to BPH patients with sera PSA levels between 4-20ng/mL and >20ng/mL. No immunoreactions to NF-κBp65 were observed in PC patients with sera PSA levels between 4-20ng/mL.
  
  
  CONCLUSIONS
  The sensibility of both NF-κB and PSA to inflammation allowed confirming the relationship between these two molecules and its involvement in prostatic diseases progression (inflammatory and neoplasic).
authors: Faculty of Sciences of Bizerte, Unit of Immunology and Microbiology Environmental and Carcinogenesis (IMEC), University of Carthage, 7021 Zarzouna, Tunisia.
authors: Y
authors: Y
authors: Bouraoui
authors: A
authors: A
authors: Ben Jemaa
authors: G
authors: G
authors: Rodriguez
authors: N
authors: N
authors: Ben Rais
authors: B
authors: B
authors: Fraile
authors: R
authors: R
authors: Paniagua
authors: S
authors: S
authors: Sellemi
authors: M
authors: M
authors: Royuela
authors: R
authors: R
authors: Oueslati
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D006801:Humans; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit / Q000032:analysis / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism* / Q000473:pathology; D017430:Prostate-Specific Antigen / Q000097:blood*; D011470:Prostatic Hyperplasia / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution; D051996:Transcription Factor RelA / Q000032:analysis / Q000378:metabolism*; D055815:Young Adult
chemical_list: D052002:NF-kappa B p50 Subunit; D051996:Transcription Factor RelA; D017430:Prostate-Specific Antigen