title: Postsynthetic modification of oligonucleotides with imidazophenazine dye and its effect on duplex stability.
abstract: Carboxyalkyl derivative of the intercalating agent imidazo[4,5-b]phenazine was used for the postsynthetic oligonucleotide modification. Model pentadecathymidylate-imidazophenazine conjugate was prepared from 5'-aminoalkyl-modified (dT)(15) by using phosphonium coupling reagent BOP in the presence of 1-hydroxybenzotriazole. Spectral-fluorescent properties of the conjugate were studied. The attachment of the dye was found to increase the thermal stability of (dT)(15) duplex with poly(dA) by more than 4°C, probably by the intercalation mechanism.
authors: Institute of Molecular Biology and Genetics, National Academy of Sciences, Kyiv, Ukraine.
authors: Larysa
authors: L
authors: Dubey
authors: Olga
authors: O
authors: Ryazanova
authors: Victor
authors: V
authors: Zozulya
authors: Dmytro
authors: D
authors: Fedoryak
authors: Igor
authors: I
authors: Dubey
mesh_terms: D004396:Coloring Agents / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D004396:Coloring Agents; D007093:Imidazoles; D007364:Intercalating Agents; D009841:Oligonucleotides; D010619:Phenazines; C029899:imidazole