title: Demonstration of vaginal colonization with GusA-expressing Lactobacillus jensenii following oral delivery in rhesus macaques.
abstract: The vaginal microbiome, which harbors beneficial Lactobacillus strains, is believed to be a major host defense mechanism for preventing infections of the urogenital tract. It has been suggested that the gastrointestinal tract serves as a reservoir for lactobacilli that colonize the vagina. Using rhesus macaques, we examined whether oral delivery of human vaginal Lactobacillus jensenii 1153-1646, a GusA-producing strain, would result in colonization of the rectum and the vagina. Lactobacilli were identified from the vagina tracts of three macaques on the basis of β-glucuronidase enzyme production, 16S rRNA gene sequence and DNA homology using a repetitive sequence-based polymerase chain reaction.
authors: Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. llagenaur@mail.nih.gov
authors: Laurel A
authors: LA
authors: Lagenaur
authors: Peter P
authors: PP
authors: Lee
authors: Dean H
authors: DH
authors: Hamer
authors: Brigitte E
authors: BE
authors: Sanders-Beer
mesh_terms: D000284:Administration, Oral; D000818:Animals; D005243:Feces / Q000382:microbiology; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D019936:Probiotics / Q000008:administration & dosage*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012007:Rectum / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014621:Vagina / Q000382:microbiology*
chemical_list: D012336:RNA, Ribosomal, 16S; D005966:Glucuronidase