title: Cooperative role of nectin-nectin and nectin-afadin interactions in formation of nectin-based cell-cell adhesion.
abstract: The nectin cell adhesion molecules interact in trans with each other through their extracellular regions and with afadin through their cytoplasmic tails, forming adherens junctions in cooperation with cadherins. In a single cell, Necl-5 (nectin-like molecule-5) localizes at the leading edge and regulates directional cell movement in response to a chemoattractant. In such a single cell, afadin also localizes at the leading edge without interacting with nectins or Necl-5. It remains unknown how the nectin-nectin and nectin-afadin interactions are initiated when moving cells contact each other to initiate the formation of adherens junctions. We show here that the Necl-5-nectin interaction induced by cell-cell contact enhances the nectin-afadin interaction. This interaction then enhances the nectin-nectin interaction, which further enhances the nectin-afadin interaction in a positive feedback manner. Thus, the Necl-5-nectin, nectin-nectin, and nectin-afadin interactions cooperatively increase the clustering of the nectin-afadin complex at the cell-cell contact sites, promoting the formation of the nectin-based cell-cell adhesion.
authors: Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Hyogo 650-0017, Japan.
authors: Souichi
authors: S
authors: Kurita
authors: Hisakazu
authors: H
authors: Ogita
authors: Yoshimi
authors: Y
authors: Takai
mesh_terms: D022005:Adherens Junctions / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007739:L Cells; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D000075983:Nectins; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D046912:Multiprotein Complexes; D000075983:Nectins; D011991:Receptors, Virus; C108835:afadin; C066100:poliovirus receptor