title: RNA polymerase II degradation in response to rapamycin is not mediated through ubiquitylation.
abstract: In Saccharomyces cerevisiae, the immunosuppressor rapamycin engenders the degradation of excessive RNA polymerase II leading to growth arrest but the regulation of this process is not known yet. Here, we show that this mechanism is dependent on the peptidyl prolyl cis/trans isomerase Rrd1. Strikingly this degradation is independent of RNA polymerase II polyubiquitylation and does not require the elongation factor Elc1. Our data reveal that there are at least two alternative pathways to degrade RNA polymerase II that depend on different type of stresses.
authors: University of Montreal, Maisonneuve-Rosemont Hospital, Research Center, 5415 de l'Assomption, Montreal, Quebec, Canada H1T 2M4.
authors: Nathalie
authors: N
authors: Jouvet
authors: Jeremie
authors: J
authors: Poschmann
authors: Julie
authors: J
authors: Douville
authors: Rim
authors: R
authors: Marrakchi
authors: Dindial
authors: D
authors: Ramotar
mesh_terms: D007166:Immunosuppressive Agents / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology*; D054875:Ubiquitination
chemical_list: D007166:Immunosuppressive Agents; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II; D019696:Peptidylprolyl Isomerase; C490203:RRD1 protein, S cerevisiae; D020123:Sirolimus