title: Lipolytic effects of B-type natriuretic peptide 1-32 in adipose tissue of heart failure patients compared with healthy controls.
abstract: OBJECTIVE
  Our goal was to examine the role of B-type natriuretic peptide (BNP) in lipolysis regulation in heart failure (HF) patients.
  
  
  BACKGROUND
  Enhanced adipose tissue lipolysis can contribute to myocardial lipid overload, insulin resistance, and cachexia in advanced HF. Natriuretic peptides were recently recognized to stimulate lipolysis in healthy subjects.
  
  
  METHODS
  Ten nondiabetic HF patients (New York Heart Association functional class III, 50% nonischemic etiology) and 13 healthy subjects (control subjects) of similar age, sex, and body composition underwent a microdialysis study of subcutaneous abdominal adipose tissue. Four microdialysis probes were simultaneously perfused with 0.1 μM BNP(1-32,) 10 μM BNP(1-32), 10 μM norepinephrine (NE) or Ringer's solution. Outgoing dialysate glycerol concentration (DGC) was measured as an index of lipolysis.
  
  
  RESULTS
  Spontaneous lipolysis was higher in HF patients compared with control subjects (DGC: 189 ± 37 μmol/l vs. 152 ± 35 μmol/l, p < 0.01). Response to NE was similar (p = 0.35) in HF patients and control subjects (DGC increase of 1.7 ± 0.2-fold vs. 1.7 ± 0.4-fold). BNP(1-32) 10 μM markedly increased lipolysis in both HF patients and control subjects (DGC increase of 2.8 ± 0.5-fold vs. 3.2 ± 0.3-fold), whereas the response to 0.1 μM BNP(1-32) was more pronounced in HF patients (p = 0.02). In HF patients, spontaneous lipolysis positively correlated with insulin resistance and the response to BNP(1-32) negatively correlated with adiposity.
  
  
  CONCLUSIONS
  BNP(1-32) exerts strong lipolytic effects in humans. Despite marked elevation of plasma immunoreactive BNP, the responsiveness of adipose tissue to BNP(1-32) is not attenuated in HF, possibly reflecting a deficiency of endogenous bioactive BNP. Lipolytic effects of BNP can contribute to excessive fatty acid mobilization in advanced HF.
authors: Department of Cardiology, Institute of Clinical and Experimental Medicine, IKEM, Videnska 1958/9, Prague, Czech Republic.
authors: Jan
authors: J
authors: Polak
authors: Martin
authors: M
authors: Kotrc
authors: Zuzana
authors: Z
authors: Wedellova
authors: Antonin
authors: A
authors: Jabor
authors: Ivan
authors: I
authors: Malek
authors: Josef
authors: J
authors: Kautzner
authors: Ludmila
authors: L
authors: Kazdova
authors: Vojtech
authors: V
authors: Melenovsky
mesh_terms: D016022:Case-Control Studies; D006152:Cyclic GMP / Q000378:metabolism; D045604:Extracellular Fluid / Q000378:metabolism; D006333:Heart Failure / Q000378:metabolism*; D006801:Humans; D008066:Lipolysis; D008297:Male; D017551:Microdialysis; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000008:administration & dosage / Q000378:metabolism*; D009638:Norepinephrine / Q000008:administration & dosage / Q000378:metabolism; D012039:Regional Blood Flow; D050507:Subcutaneous Fat, Abdominal / Q000098:blood supply / Q000378:metabolism*
chemical_list: D020097:Natriuretic Peptide, Brain; D006152:Cyclic GMP; D009638:Norepinephrine