title: Screening and detection of biomarkers in chickpea plants exposed to chromium and cadmium.
abstract: A broad screening protocol, covering the most general phytochemical groups of compounds, was developed on the basis of high performance thin layer chromatography (HPTLC). A total of six TLC systems, comprising three derivatization reagents, two stationary phases and two mobile phases, were included. The screening method was applied for the identification of biomarkers in the chickpea plant exposed to cadmium and chromium. The biomarkers were selected on the basis of significant changes (0.26-4.6 fold) in concentration levels of phytochemicals. Totally, five different amino acids, three organic acids, one sulphur containing compound and one sugar were identified as biomarkers in chickpea exposed heavy metal.
authors: Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi-1 10062, India.
authors: Mather Ali
authors: MA
authors: Khan
authors: Mauji
authors: M
authors: Ram
authors: Prabhakar
authors: P
authors: Jha
authors: M Mobeen
authors: MM
authors: Ahmad
authors: Pravej
authors: P
authors: Alam
authors: Kamaluddin
authors: Athar
authors: A
authors: Ali
authors: Usha
authors: U
authors: Kiran
authors: M Z
authors: MZ
authors: Abdin
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D002857:Chromium / Q000633:toxicity*; D029863:Cicer / Q000187:drug effects* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002104:Cadmium; D002857:Chromium