title: Breeding of a new wastewater treatment yeast by genetic engineering.
abstract: We previously developed a host vector system for the wastewater treatment yeast Hansenula fabianii J640. The promoter and terminator regions of the gene encoding glucoamylase from H. fabianii J640 were used for a new expression vector, pHFGE-1. The performance of pHFGE-1 was compared with that of the widely used pG-1 transformant vector. H. fabianii J640 (HF-TAMY) cells were transformed with pHFGE-1, and Saccharomyces cerevisiae YPH-499 (SC-TAMY) cells were transformed with pG-1, both of which carried the Taka-amylase. Expression of Taka-amylase by HF-TAMY showed higher than that by SC-TAMY. By using this new system, we bred the new wastewater treatment yeast that shows α-amylase activity. This yeast appears to grow well under experimental wastewater conditions, and is effective in treating model wastewater containing soluble and insoluble starch.
authors: Graduate School of Biosphere Science, Hiroshima University, 1-4-4, Kagamiyama, Higashihiroshima, Hiroshima 739-8527, Japan. iefuji@nrib.go.jp.
authors: Miyoshi
authors: M
authors: Kato
authors: Haruyuki
authors: H
authors: Iefuji