title: Note: Direct force and ionic-current measurements on DNA in a nanocapillary.
abstract: We have developed optical tweezers, with force measurements based on fast video tracking, for analysis and control of DNA translocation through nanocapillaries. Nanocapillaries are single-molecule biosensors with very similar characteristics to solid-state nanopores. Our novel experimental setup allows for ionic-current measurements in which the nanocapillary is oriented perpendicular to the trapping laser. Using video-based particle tracking, we are able to measure the position of DNA coated colloids at sub-millisecond resolution and in real-time. We present the first electrophoretic force and simultaneous ionic-current measurements of a single DNA molecule inside the orifice of a nanocapillary.
authors: Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom.
authors: O
authors: O
authors: Otto
authors: L J
authors: LJ
authors: Steinbock
authors: D W
authors: DW
authors: Wong
authors: J L
authors: JL
authors: Gornall
authors: U F
authors: UF
authors: Keyser
mesh_terms: D010582:Bacteriophage lambda; D004279:DNA, Viral / Q000032:analysis*; D004553:Electric Conductivity; D009038:Motion; D049329:Nanostructures; D052898:Optical Tweezers
chemical_list: D004279:DNA, Viral