title: Age-dependent expression of forkhead box O proteins in the duodenum of rats.
abstract: The O subfamily of forkhead box (FoxO) proteins is the downstream effector of the insulin-like growth factor-1/phosphoinositide 3-kinase/protein kinase B (IGF-1/PI3K/PKB) signal pathway. The objective of the present study was to examine the expressions of three members of FoxO proteins, FoxO1, FoxO3a, and FoxO4 in the duodenum of Sprague-Dawley rats at different ages. The result demonstrated that the expression of FoxO4 in rat duodenum showed an age-dependent manner. At Day 21, there were no detectable localization and expression of FoxO4 in the duodenum, while, at Months 2 and 6, localization and expression of FoxO4 were distinct. In addition, FoxO4 staining was primarily concentrated in the cell nuclei of the lamina propria around the intestinal gland of the duodenum in 2-month-old rats, but was not detectable in the same area in 6-month-old rats. Our results showed also that although FoxO3a existed in the cytoplasm of the lamina propria at a low level at the 2- and 6-month marks, it was still not detectable at Day 21. Besides, FoxO1 was not detectable in all parts and stages. Taken together, our findings suggested that the cell-specific and age-dependent expressional patterns of FoxO4 and FoxO3a proteins in the duodenum play some roles in the development and growth performance of the rat duodenum.
authors: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors: Pan
authors: P
authors: Huang
authors: Zhen-qi
authors: ZQ
authors: Zhou
authors: Rui-hua
authors: RH
authors: Huang
authors: Bo
authors: B
authors: Zhou
authors: Quan-wei
authors: QW
authors: Wei
authors: Fang-xiong
authors: FX
authors: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis; D003593:Cytoplasm / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism / Q000473:pathology*; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D009092:Mucous Membrane / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D000071161:Forkhead Box Protein O1
chemical_list: D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009419:Nerve Tissue Proteins; C499515:FOXO3 protein, rat; C524737:FOXO4 protein, rat; C074639:Foxo1 protein, rat; D000071161:Forkhead Box Protein O1