title: [Human single chain antibodies directed to tumor necrosis factor].
abstract: Six unique phage antibodies to human TNF have been selected from a combinatorial library of human single chain fragment variable. ELISA and Western-blotting was used to study selected phage antibodies binding with TNF. The specificity of selected antibodies was determined by binding with interferon alpha and gamma, bovine serum albumin, ovalbumin and ubiquitin. Two antibodies, sA1 and sB3, were converted into a soluble single-chain antibody form and their affinity was 2.5 and 13.7 nM respectively.
authors: M A
authors: MA
authors: Vikhrova
authors: T A
authors: TA
authors: Batanova
authors: L R
authors: LR
authors: Lebedev
authors: L N
authors: LN
authors: Shingarova
authors: L A
authors: LA
authors: Frank
authors: M P
authors: MP
authors: Kirpichnikov
authors: N V
authors: NV
authors: Tikunova
mesh_terms: D000595:Amino Acid Sequence; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D019151:Peptide Library; D012710:Serum Albumin, Bovine / Q000276:immunology; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D048069:Tumor Necrosis Factors / Q000276:immunology*; D025801:Ubiquitin / Q000276:immunology
chemical_list: D016207:Cytokines; D019151:Peptide Library; D057127:Single-Chain Antibodies; D048069:Tumor Necrosis Factors; D025801:Ubiquitin; D012710:Serum Albumin, Bovine; D010047:Ovalbumin