title: Differential brain angiotensin-II type I receptor expression in hypertensive rats.
abstract: Blood-borne angiotensin-II (Ang-II) has profound effects in the brain. We tested the hypothesis that Ang-II-dependent hypertension involves differential Ang-II type I (AT(1)) receptors expression in the subfornical organ (SFO) and the rostral ventrolateral medulla (RVLM). Male Wistar rats were implanted with 14-day osmotic minipump filled with Ang-II (150 ng/kg/min) or saline. AT(1) receptor mRNA levels were detected in the SFO and RVLM by reverse transcription-polymerase chain reaction (RT-PCR). Ang-II caused hypertension (134 ± 10 mmHg vs. 98 ± 9 mmHg, n = 9, p < 0.05). RT-PCR revealed that Ang-II infusion induced increased AT(1) receptor mRNA levels in RVLM and decreased in SFO. Our data suggest that Ang-II-induced hypertension involves differential expression of brain AT(1) receptors.
authors: Veterinary Sciences Department, Center for Agrarian Sciences, Federal University of Paraíba, Areia, PB 58397-000, Brazil. valdir@cca.ufpb.br
authors: Valdir A
authors: VA
authors: Braga
mesh_terms: D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013356:Subfornical Organ / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II