title: Immobilization and stabilization of papain on poly(hydroxyethyl methacrylate-ethylenglycol dimethacrylate) beads grafted with epoxy functional polymer chains via surface-initiated-atom transfer radical polymerization (SI-ATRP).
abstract: Poly(hydroxyethyl methacrylate-ethylen glycol dimethacrylate), p(HEMA-EGDMA), beads were prepared by suspension polymerization, and were decorated with fibrous poly(glycidyl methacrylate), p(GMA), via surface initiated-atom transfer radical polymerization (SI-ATRP). The functional epoxy groups of the beads were used for covalent immobilization of papain. The average amount of immobilized enzyme was 18.7 mg/g beads. The immobilized enzyme was characterized by temperature, pH, operational and storage stability experiments. The maximum velocity of the free and immobilized enzymes (V(max)) and Michaelis-Menten constant (K(m)) values were determined as 10.7 and 8.3 U/mg proteins and 274 and 465 μM, respectively. The immobilized papain was operated in a batch reactor, and it was very effective for hydrolysis of different proteins (i.e., casein and cytochrom c).
authors: Gazi University, Faculty of Sciences, Biochemical Processing and Biomaterial Research Laboratory, 06500 Teknikokullar, Ankara, Turkey. g_bayramoglu@hotmail.com
authors: Gulay
authors: G
authors: Bayramoglu
authors: B Filiz
authors: BF
authors: Senkal
authors: Meltem
authors: M
authors: Yilmaz
authors: M Yakup
authors: MY
authors: Arica
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008863:Microspheres; D010206:Papain / Q000378:metabolism*; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry*; D059027:Recycling; D013499:Surface Properties; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D004852:Epoxy Compounds; D011109:Polymethacrylic Acids; C107571:poly(ethyleneglycol dimethacrylate-2-hydroxyethylmethacrylate); D010206:Papain