title: [The effects of mechanical stretching on phospholipase C-γ1 of human periodontal ligament cells].
abstract: OBJECTIVE
  To investigate the effects of mechanical stretching on concentration of phospholipase C-γ1(PLC-γ1) in the human periodontal ligament cells(HPDLCs).
  
  
  METHODS
  Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time. PLC-γ1 was examined through flow cytometry. The data were analyzed by one-way ANOVA using SPSS13.0 software package.
  
  
  RESULTS
  In early period of stretching, the expression of PLC-γ1 remained at a lower level. With the stretching time prolonging, the expression of PLC-γ1 increased gradually. The level of PLC-γ1 of each stretching group reached peak concentration at 50-minute (P<0.01), then declined at 60-minute (P<0.01).
  
  
  CONCLUSIONS
  Stretching force induces the change of concentration of the PLC-γ1 of HPDLCs.
authors: Department of Prosthodontics, Shanghai Jiao Tong University School of Medicine, Shanghai, China. li_junn@163.com
authors: Jun
authors: J
authors: Li
authors: Chun
authors: C
authors: Xu
authors: Yi
authors: Y
authors: Hao
authors: Xiao-feng
authors: XF
authors: Liu
mesh_terms: D001696:Biomechanical Phenomena; D006801:Humans; D010513:Periodontal Ligament; D051966:Phospholipase C gamma
chemical_list: D051966:Phospholipase C gamma