title: Mitigation of 2,4-dinitrofluorobenzene-induced atopic dermatitis-related             symptoms by Terminalia chebula Retzius.
abstract: To evaluate whether an aqueous seed extract of Terminalia chebula Retzius inhibited development of atopy in vivo, we used a 2,4-dinitrofluorobenzene (DNFB)-induced animal model of atopic symptoms to investigate the effects of the extract. We measured CD4+ cell numbers by hematoxylin and eosin (H&E) staining, and determined the expression levels of matrix metalloproteinase (MMP)-9, interleukin (IL)-31, and T-bet genes, in this animal model. The data showed that a Terminalia chebula extract (100 µg/ml) exhibited strong anti-atopic activity, mediating a 52% reduction in the immune response, as measured by thickness of ear swelling, and resulting in decreased eosinophil levels in adjacent skin tissue. Collectively, the results indicate that a Terminalia chebula seed extract has potential for alleviation of atopy-like symptoms induced by DNFB in the mouse.
authors: Department of Food Science and Biotechnology, Kyungpook National                     University, Daegu 702-701, Republic of Korea.
authors: Dong-Yoon
authors: DY
authors: Nam
authors: Jin-Man
authors: JM
authors: Lee
authors: Jin-Chul
authors: JC
authors: Heo
authors: Sang-Han
authors: SH
authors: Lee
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000009:adverse effects; D004801:Eosine Yellowish-(YS); D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006416:Hematoxylin; D007378:Interleukins / Q000032:analysis / Q000096:biosynthesis; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012639:Seeds / Q000737:chemistry; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D020825:T-Box Domain Proteins / Q000032:analysis / Q000096:biosynthesis; D029766:Terminalia / Q000737:chemistry*; D000098815:T-bet Transcription Factor
chemical_list: D007378:Interleukins; D010936:Plant Extracts; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; C502588:interleukin-31, mouse; D004139:Dinitrofluorobenzene; D020780:Matrix Metalloproteinase 9; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin