title: CYFIP dependent actin remodeling controls specific aspects of Drosophila eye morphogenesis.
abstract: Cell rearrangements shape organs and organisms using molecular pathways and cellular processes that are still poorly understood. Here we investigate the role of the Actin cytoskeleton in the formation of the Drosophila compound eye, which requires extensive remodeling and coordination between different cell types. We show that CYFIP/Sra-1, a member of the WAVE/SCAR complex and regulator of Actin remodeling, controls specific aspects of eye architecture: rhabdomere extension, rhabdomere terminal web organization, adherens junctions, retina depth and basement membrane integrity. We demonstrate that some phenotypes manifest independently, due to defects in different cell types. Mutations in WAVE/SCAR and in ARP2/3 complex subunits but not in WASP, another major regulator of Actin nucleation, phenocopy CYFIP defects. Thus, the CYFIP-SCAR-ARP2/3 pathway orchestrates specific tissue remodeling processes.
authors: Fovea-Pharmaceuticals, Institut de la vision, 17 rue Moreau 75012 Paris, France.
authors: Anne
authors: A
authors: Galy
authors: Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Donders Institute for Brain, Cognition and Behaviour & Radboud University Nijmegen Medical Center, 6525 GA Nijmegen, The Netherlands. Electronic address: A.Schenck@antrg.umcn.nl.
authors: Annette
authors: A
authors: Schenck
authors: Department of Molecular Biology and Genetics, Bogazici University, Bebek, Istanbul, Turkey.
authors: H Bahar
authors: HB
authors: Sahin
authors: Department of Human Genetics, Emory University School of Medicine, Atlanta, GA, USA.
authors: Abrar
authors: A
authors: Qurashi
authors: INSERM, U968, Université Pierre et Marie Curie-Paris 6, UM80, Institut de la Vision, CNRS, UMR-7210, Fondation Ophtalmologique Adolphe de Rothschild, Paris, France; Institute of Ophthalmology, University College of London, London, United Kingdom.
authors: José-Alain
authors: JA
authors: Sahel
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/UDS, BP 10142, 67404 Illkirch, CU de Strasbourg, France.
authors: Céline
authors: C
authors: Diebold
authors: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/UDS, BP 10142, 67404 Illkirch, CU de Strasbourg, France. Electronic address: angela@igbmc.fr.
authors: Angela
authors: A
authors: Giangrande
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology*; D022005:Adherens Junctions / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins / Q000502:physiology*; D005123:Eye / Q000196:embryology*; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009154:Mutation
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D029721:Drosophila Proteins; C484666:Sra-1 protein, Drosophila