title: Effect of hematocrit on accuracy of two point-of-care glucometers for use in dogs.
abstract: OBJECTIVE
  To determine the effect of Hct on blood glucose readings of dogs obtained by use of 2 point-of-care (POC) blood glucometers and a laboratory analyzer.
  
  
  METHODS
  184 dogs, including 139 Greyhounds.
  
  
  METHODS
  Venous blood samples collected from 184 dogs with a range of Hcts (measured in EDTA-anticoagulated blood) were immediately analyzed with a handheld glucometer specifically developed for veterinary use and a glucometer developed for use in humans. The remainder of each blood sample was placed in fluoride oxalate tubes, and plasma glucose concentration was measured with a laboratory analyzer. Agreement between results for the POC glucometers and laboratory analyzer and effect of Hct on glucometer accuracy was assessed via regression analysis.
  
  
  RESULTS
  Significant differences were detected between results of the glucometers and the reference laboratory analyzer. The Hct affected the correlation between results for the glucometers and the laboratory analyzer. Deviations of the glucometers from the reference interval varied with Hct. The glucometer for veterinary use more closely correlated with the glucose concentration when Hct was within or above its reference interval. The glucometer for use in humans more closely approximated laboratory reference glucose concentrations in anemic dogs.
  
  
  CONCLUSIONS
  Hct had a relevant impact on the correlation between whole blood and plasma glucose concentrations in dogs. Significant variations between results obtained with the 2 glucometers could be critical when interpreting blood glucose measurements or selecting a POC glucometer for an intensive care setting and precise glycemic control in critically ill dogs.
authors: Department of Veterinary Clinical Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6155, Australia. a.paul@murdoch.edu.au
authors: Amanda E H
authors: AE
authors: Paul
authors: Robert E
authors: RE
authors: Shiel
authors: Florence
authors: F
authors: Juvet
authors: Carmel T
authors: CT
authors: Mooney
authors: Caroline S
authors: CS
authors: Mansfield
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D001786:Blood Glucose / Q000032:analysis*; D004285:Dogs / Q000097:blood*; D006400:Hematocrit / Q000295:instrumentation / Q000379:methods* / Q000662:veterinary; D006801:Humans; D016014:Linear Models; D019095:Point-of-Care Systems; D012016:Reference Values; D015203:Reproducibility of Results
chemical_list: D001786:Blood Glucose