title: Depression by a green tea extract of alcohol-induced oxidative stress and lipogenesis in rat liver.
abstract: We determined the effects of a green tea extract with 36% alcohol on the blood alcohol content, oxidative stress, lipogenesis, inflammation and liver function of female Wistar rats. Tea alcohol significantly decreased the O₂⁻, H₂O₂ and HOCl amounts via catechins and not caffeine. Thirty days of alcohol gavage improved the level of reactive oxygen species (ROS) in the liver, bile and blood, increased the 4-hydroxynonenal-protein adducts, Kupffer cell infiltration and lipid accumulation in the liver, and elevated the plasma alanine aminotransferase level. A western blot analysis showed reduced expression of the oxidative enzymes (CYP2E1 and NADPH oxidase p47phox protein) and lipogenic enzymes (SREBP-1c and fatty acid synthase) in the alcohol-treated liver. Tea alcohol significantly attenuated these elevated parameters. We conclude that the green tea extract in alcohol efficiently reduced the amounts of O₂⁻, H₂O₂ and HOCl primarily due to the catechin content, and not caffeine. The developed tea liquor attenuated alcohol-induced oxidative injury and lipogenesis in the liver by the synergetic action of catechins and caffeine.
authors: Division of General Surgery, Far-Eastern Memorial Hospital, Taipei, Taiwan.
authors: Kuo-Hsin
authors: KH
authors: Chen
authors: Ping-Chia
authors: PC
authors: Li
authors: Wei-Hsiang
authors: WH
authors: Lin
authors: Chiang-Ting
authors: CT
authors: Chien
authors: Boon-Hua
authors: BH
authors: Low
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000428:Alcohol Drinking / Q000009:adverse effects / Q000097:blood*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D015153:Blotting, Western; D002110:Caffeine / Q000097:blood / Q000494:pharmacology; D002392:Catechin / Q000097:blood / Q000494:pharmacology*; D019392:Cytochrome P-450 CYP2E1 / Q000097:blood; D000431:Ethanol / Q000009:adverse effects / Q000097:blood / Q000494:pharmacology; D064429:Fatty Acid Synthases / Q000097:blood; D005260:Female; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D007728:Kupffer Cells / Q000166:cytology / Q000187:drug effects; D050155:Lipogenesis / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D019255:NADPH Oxidases / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000097:blood; D013662:Tea / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D051780:Sterol Regulatory Element Binding Protein 1; D013662:Tea; D002110:Caffeine; D000431:Ethanol; D002392:Catechin; D006861:Hydrogen Peroxide; D019392:Cytochrome P-450 CYP2E1; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; D064429:Fatty Acid Synthases; D000410:Alanine Transaminase