title: Subcellular scaled multiplexed protein patterns for single cell cocultures.
abstract: Tip-based direct protein printing is a relatively new technique that is useful for controlling the cellular microenvironment with subcellular resolution. Coculture studies have been useful for mimicking the in vivo environment and studying effects on stem or progenitor cell function. However, there are many experimental variables that cannot be properly controlled and may lead to confounding results. Here we demonstrate a technique that allows spatial control of multiple cell types at single cell levels on a substrate. Specifically, 3T3 fibroblasts and C2C12 myoblasts and their respective binding dynamics with fibronectin and laminin demonstrate the single cell coculture concept.
authors: Nanofabrication Systems Division, NanoInk, Skokie, IL 60077, USA. jcollins@nanoink.net
authors: John M
authors: JM
authors: Collins
authors: Saju
authors: S
authors: Nettikadan
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D041681:NIH 3T3 Cells; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D005353:Fibronectins; D007797:Laminin