title: Efficient bone regeneration induced by bone morphogenetic protein-2 released from apatite-coated collagen scaffolds.
abstract: Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. Clinically utilized BMP-2 uses a type-I collagen scaffold as a carrier. Here we hypothesized that an apatite coating on a type-I collagen scaffold would prolong the BMP-2 release period and enhance bone regeneration in calvarial defects in mice. Apatite coating was achieved by incubating collagen scaffolds in simulated body fluid. BMP-2 release kinetics and bioactivity were evaluated by enzyme-linked immunosorbent assay and alkaline phosphatase activity measurement of cultured osteoblasts. Computed tomography and histomorphometry were performed eight weeks after various doses of BMP-2 were delivered to mouse calvarial defects using either non-modified or apatite-coated collagen scaffolds. Apatite-coated collagen scaffolds released 91.8 ± 11.5% of the loaded BMP-2 over 13 days in vitro, whereas non-modified collagen scaffolds released 98.3 ± 2.2% over the initial one day. The in vivo study showed that BMP-2 delivery with apatite-coated collagen scaffolds resulted in a significantly greater bone formation area and higher bone density than that with non-modified collagen scaffolds. This study suggests that simple apatite coating on collagen scaffolds can enhance the bone regeneration efficacy of BMP-2 released from collagen scaffolds.
authors: a Department of Bioengineering , Hanyang University , Seoul , 133-791 , South Korea.
authors: Hee Seok
authors: HS
authors: Yang
authors: Wan-Geun
authors: WG
authors: La
authors: Jooyeon
authors: J
authors: Park
authors: Chang-Sung
authors: CS
authors: Kim
authors: Gun-Il
authors: GI
authors: Im
authors: Byung-Soo
authors: BS
authors: Kim
mesh_terms: D000818:Animals; D001031:Apatites / Q000737:chemistry; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D004195:Disease Models, Animal; D065546:Drug Liberation; D006133:Growth Substances / Q000008:administration & dosage*; D007700:Kinetics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology / Q000502:physiology; D051381:Rats; D012886:Skull / Q000187:drug effects / Q000293:injuries / Q000473:pathology / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry; D016896:Treatment Outcome
chemical_list: D001031:Apatites; D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D006133:Growth Substances; D003094:Collagen