title: Establishment of a confluent cardiomyocyte culture in a cylindrical microchannel.
abstract: We established a confluent cardiomyocyte culture method using an 800-µm diameter cylindrical microchannel in this report. This was realized by introducing cardiomyocytes 2 times before and after turning over a microchip. The optimum condition was starting the flowing medium 2.0 h after seeding and flowing the medium at 1.0 µL/min. By applying this technology to a cardiomyocyte-based spherical heart pump device, one may develop self-fluid regulated devices that could be applied for implantable or circulation analysis device on a chip.
authors: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan.
authors: Yo
authors: Y
authors: Tanaka
authors: Hiroto
authors: H
authors: Akaike
authors: Yasuhiko
authors: Y
authors: Sugii
authors: Takehiko
authors: T
authors: Kitamori
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D007260:Infusion Pumps; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar