title: Preparation of HCV NS3 and NS5B proteins to support small-molecule drug discovery.
abstract: Production of high-quality, well-characterized recombinant proteins facilitates screening of compound libraries. The protocols detailed in this unit are used to purify three recombinant enzymes that are widely used in HCV research: the HCV NS3 protease domain, the helicase domain as an NS3+NS4A complex, and the NS5B RNA-dependent RNA polymerase. The active enzymes are purified to homogeneity by two-column chromatography to support a screening program for HCV inhibitors.
authors: Gilead Sciences, Foster City, CA, USA.
authors: Magdeleine
authors: M
authors: Hung
authors: Ruth
authors: R
authors: Wang
authors: Xiaohong
authors: X
authors: Liu
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016174:Hepacivirus / Q000187:drug effects / Q000201:enzymology*; D066298:In Vitro Techniques; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins