title: Aptamer-targeted magnetic nanospheres as a solid-phase extraction sorbent for determination of ochratoxin A in food samples.
abstract: A sorbent based on the aptamer for ochratoxin A was immobilized onto magnetic nanospheres (MNS) and used to develop a magnetic solid-phase extraction procedure to clean up food samples in conjunction with high-performance liquid chromatography separation and fluorescence detection. Specific retention of ochratoxin A by the sorbent was demonstrated, and the capacity of the MNS-aptamer sorbent was determined. The efficacy of this new approach was successfully evaluated through comparison with solid-phase extraction on commercial C18 cartridge. Several different food samples fortified in the range of with 2.5-50 μg/kg yielded mean recoveries from 67% to 90%, respectively. Finally, this oligosorbent was applied to the selective extraction of ochratoxin A from unfortified food samples.
authors: College of Chemistry and Molecular Sciences, Wuhan University, Luojiashan, Wuhan, China. wxmdwb927@126.com
authors: Ximei
authors: X
authors: Wu
authors: Jun
authors: J
authors: Hu
authors: Binghui
authors: B
authors: Zhu
authors: Lin
authors: L
authors: Lu
authors: Xiangdong
authors: X
authors: Huang
authors: Daiwen
authors: D
authors: Pang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D058185:Magnetite Nanoparticles; D009793:Ochratoxins; C025589:ochratoxin A