title: [Light-sheet based fluorescence microscopy: the dark side of the sample finally revealed].
abstract: Light-sheet based fluorescence microscopy (LSM) is an optical technique that becomes more and more popular for multi-view imaging of in vivo sample in its physiological environment. LSM combines the advantages of the direct optical sectioning to the ones of optical tomography by angular scanning. In fact, a thin light-sheet illuminates laterally a section of the sample, thus limiting the effects of photobleaching and phototoxicity only to the plane of interest. The spatial resolution can be improved by combining multiple views obtained along different angle into a single data, leading to a 3D isotropic rendering of the sample. Such an approach provides several advantages in comparison to conventional 3D microscopic techniques: confocal and multiphoton microscopies. It makes LSM an optical tool suited for imaging specimens with a subcellular resolution even inside an embryo and with temporal resolution adapted for real-time monitoring of biological processes.
authors: Université Paris Descartes, 75270 Paris Cedex 06, France. philippe.girard@parisdescartes.fr
authors: Philippe P
authors: PP
authors: Girard
authors: Benoît C
authors: BC
authors: Forget
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000648:ultrastructure; D004867:Equipment Design; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D038761:Photobleaching; D010777:Photochemistry; D013048:Specimen Handling; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods; D015027:Zebrafish / Q000196:embryology