title: UVC mutagenicity is suppressed in Japanese miso-treated human RSa cells, possibly via GRP78 expression.
abstract: Little is known about the ability of miso, to modulate mutability in human cells. We have observed increased levels of glucose-regulated protein 78 (GRP78) expression in association with suppression of mutation in human RSa cells irradiated with ultraviolet C (UVC). Here we examined to determine whether miso treatment results in increased GRP78 expression and suppression of UVC mutagenicity in RSa cells. Supernatants of water extracts of miso products and their components were tested. In the sample-treated cells, the amount of GRP78, as estimated by RT-PCR and immunoblotting analysis, increased, and the UVC-induced ouabain resistant mutation (Oua(R)) and the K-ras codon 12-base substitution mutation frequency decreased. This decrease was not observed in cells with downregulation of GRP78 by GRP78 siRNA transfection. The results suggest that miso suppresses UVC mutagenicity by increasing GRP78 expression in human cells.
authors: Department of Environmental Biochemistry, Graduate School of Medicine, Chiba University.
authors: Xia
authors: X
authors: Jiang
authors: Qian
authors: Q
authors: Ren
authors: Shi-Ping
authors: SP
authors: Chen
authors: Xiao-Bo
authors: XB
authors: Tong
authors: Mei
authors: M
authors: Dong
authors: Shigeru
authors: S
authors: Sugaya
authors: Takeshi
authors: T
authors: Tanaka
authors: Kazuko
authors: K
authors: Kita
authors: Nobuo
authors: N
authors: Suzuki
mesh_terms: D015153:Blotting, Western; D002461:Cell Line, Transformed; D003062:Codon; D000091342:Endoplasmic Reticulum Chaperone BiP; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D020868:Gene Silencing / Q000187:drug effects; D011905:Genes, ras / Q000187:drug effects / Q000528:radiation effects; D006360:Heat-Shock Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007564:Japan; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects; D059645:Mutation Rate; D010042:Ouabain / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045730:Soy Foods; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D003062:Codon; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D010936:Plant Extracts; D034741:RNA, Small Interfering; D010042:Ouabain