title: Periodontal healing by periodontal ligament cell sheets in a teeth replantation model.
abstract: OBJECTIVE
  Successful transplantation of avulsed teeth is to restore the attachment and regenerate the periodontal support. Different strategies have been applied in treatment from modification of teeth storage, antibiotic usage to peridontium tissue replacement. We developed a novel periodontal ligament cell-sheet delivery system to apply on delayed replanted teeth in promoting periodontal healing in a canine model.
  
  
  METHODS
  Autologous periodontal ligament (PDL) fibroblasts were isolated from extracted premolars of beagle dog. The cell-sheets were fabricated using normal culture dish after stimulation of extracellular matrix formation. Teeth were surgically extracted and attached soft tissues were removed. After root canal treatment, the root of teeth were wrapped by the PDL cell-sheets and replanted back to prior socket accordingly whilst teeth without cell sheets as a control. Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Regeneration of periodontal tissue was evaluated through histology assay.
  
  
  RESULTS
  Multi-layered PDL cell-sheet could be attached on tooth root and most cells on sheet-tooth constructs were viable before replantation. Minimum clinical signs of inflammation were observed in experiment. PDL cell-sheets group show significant higher occurrence of favourable healing (88.4%) than control group with low healing (5.3%). Periodontal ligament and cememtum tissue regeneration was observed in the experimental group, and the regenerated tissues showed high collagen type III, type I and fibronectin expression.
  
  
  CONCLUSIONS
  The periodontal ligament cell-sheets fabricated through normal cell culture dish has a potential for regeneration of periodontal ligament and may become a novel therapy for avulsed teeth replantation.
authors: Department of Cellular Biology, School of Biological Sciences and Technology, Xiangya School of Medicine, Central South University, PR China.
authors: Yefang
authors: Y
authors: Zhou
authors: Yusheng
authors: Y
authors: Li
authors: Ling
authors: L
authors: Mao
authors: Hao
authors: H
authors: Peng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005347:Fibroblasts / Q000637:transplantation; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D010513:Periodontal Ligament / Q000166:cytology / Q000637:transplantation*; D010519:Periodontium / Q000502:physiology / Q000601:surgery; D023822:Tissue Engineering; D014084:Tooth Avulsion / Q000601:surgery*; D014090:Tooth Replantation / Q000379:methods*; D014945:Wound Healing