title: Presence of murine leukemia virus (MLV)-related virus gene sequences in a commercial RT-PCR reagent.
abstract: BACKGROUND
  The recent identification of murine leukemia virus (MLV)-related viruses in patients with chronic fatigue syndrome (CFS) has aroused much interest, not least among sufferers. However, other studies failed to detect these viruses in CFS patients.
  
  
  METHODS
  We wanted to establish a MLV-related virus real-time PCR for routine diagnostics.
  
  
  RESULTS
  Our study identified false positive MLV-related virus results due to a contamination of Superscript III Platinum One-Step Quantitative RT-PCR System (Invitrogen).
  
  
  CONCLUSIONS
  This observation may be helpful to elucidate discrepant results for the detection of MLV-related virus like xenotropic MLV-related virus (XMRV) in recently published studies.
authors: Medical Laboratory Bremen, Bremen, Germany. dietmar.wolff@mlhb.de
authors: Dietmar
authors: D
authors: Wolff
authors: Andreas
authors: A
authors: Gerritzen
mesh_terms: D001769:Blood / Q000821:virology; D003199:Computer Systems; D018076:DNA, Complementary / Q000032:analysis*; D004340:Drug Contamination; D005189:False Positive Reactions; D015750:Genes, gag; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D009052:Leukemia Virus, Murine / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D007202:Indicators and Reagents; D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic