title: Lack of association between chemokine receptor 5 (CCR5) δ32 mutation and pathogenesis of asthma in Iranian patients.
abstract: BACKGROUND
  Chemokines and their receptors are clinically important mediators, as the chemokine receptors are expressed on almost all immune cells. They play pivotal roles in pathogenesis of almost all clinical situations including asthma. Correspondingly, MIP-1α (CCL3), MIP-1β (CCL4), and RANTES (CCL5) are among the important chemokines involved in the pathogenesis of asthma. These chemokines bind to the CCR5 (their related receptor) on the cell surfaces. Attachment of related chemokine ligands to CCR5 plays an important role in the pathogenesis of asthma; hence, this study aimed to analyze δ32 mutations in CCR5 in asthmatic patients.
  
  
  METHODS
  This experimental study was undertaken on 162 asthmatic patients and 200 healthy controls during February to June 2008 at Rafsanjan University of Medical Sciences. The Gap-PCR method was applied to analyze the δ32 mutation in the CCR5 gene, and demographic data (eg, age, sex, occupation, socio-economic status) were collected using a questionnaire.
  
  
  RESULTS
  The findings of this study indicated that none of the asthmatic patients exhibited δ32 mutation in CCR5 chemokine receptor while only 3 (1.5%) of controls had the heterozygotic form of this mutation.
  
  
  CONCLUSIONS
  Several research groups analyzed δ32 mutations in CCR5 in different diseases, including asthma. Some investigations reported a significant relation between asthma and δ32 mutations in CCR5, but there are also many reports which failed to find a relation between asthma and this mutation. Based on the results of this study and others, it seems that the δ32 mutation does not affect the pathogenesis of asthma.
authors: Department of Infectious Disease, Rafsanjan Ali Ebne Abitaleb Hospital, Rafsanjan, Iran.
authors: Hamid
authors: H
authors: Abousaidi
authors: Reza
authors: R
authors: Vazirinejad
authors: Mohammad Kazemi
authors: MK
authors: Arababadi
authors: Houshang
authors: H
authors: Rafatpanah
authors: Ali Akbar
authors: AA
authors: Pourfathollah
authors: Reza
authors: R
authors: Derakhshan
authors: Saeed
authors: S
authors: Daneshmandi
authors: Gholamhossein
authors: G
authors: Hassanshahi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001249:Asthma / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019713:Receptors, CCR5 / Q000235:genetics*; D055815:Young Adult
chemical_list: D019713:Receptors, CCR5; D007073:Immunoglobulin E