title: Prenatal lead acetate exposure induces apoptosis and changes GFAP expression during spinal cord development.
abstract: Lead is an important heavy metal pollutant in the environment, and it induces neurodevelopmental toxicity, which is characterized by histological, ultrastructural, and neurochemical changes in the central nervous system. The aim of this study was to evaluate the effects of prenatal acute lead exposure on apoptosis, GFAP expression, and lead deposition in the developing spinal cord. Chick embryos were exposed to 150μg or 450μg doses of lead acetate via yolk sac at E3 or E5 embryonic ages and incubated for six days. Lead deposition was observed in the ependymal cells, developing dorsal, and ventral horns, and in the white matter of all the exposed embryos. TUNEL-positive cells were found in all layers of the spinal cord of the control and treated embryos, and lead exposure resulted in a significant increase in the numerical density of the apoptotic cells. Control embryos showed intense GFAP expression in the ependymal cells of the roof and floor plates, and in the gray and white matters; whereas exposure to lead reduced GFAP reactivity. In ovo lead exposure induces apoptosis, and reduces GFAP expression in the nervous system of the chick embryos, which may cause impairments during neuronal development and consequences in childhood and adulthood.
authors: Departamento de Biologia Celular, Universidade Federal de Santa Catarina, Trindade, Florianópolis, SC, Brazil. yararm@ccb.ufsc.br
authors: Yara Maria Rauh
authors: YM
authors: Müller
authors: Karoline
authors: K
authors: Kobus
authors: Janaína Chaves
authors: JC
authors: Schatz
authors: Dib
authors: D
authors: Ammar
authors: Evelise Maria
authors: EM
authors: Nazari
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002642:Chick Embryo; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D015386:Hazardous Substances / Q000633:toxicity*; D020287:In Situ Nick-End Labeling; D009420:Nervous System / Q000187:drug effects / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009942:Organometallic Compounds / Q000633:toxicity*; D013116:Spinal Cord / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D015386:Hazardous Substances; D009942:Organometallic Compounds; C008261:lead acetate