title: Analysis of Porphyromonas gingivalis PG27 by deletion and intragenic suppressor mutation analyses.
abstract: PG27 is required for secretion of virulence factor gingipains, and has recently been proposed as LptO, which is involved in O-deacylation of lipopolysaccharide. In the present study, a predicted 14 anti-parallel β-strand structure of PG27 was ascertained. Deletion study showed that the region from Asp382 to the C-terminal His391 of PG27 is dispensable for the function of PG27. Analysis of C-terminal deletion mutants revealed that the region in strand S14 (Asn369-Gly385) is important for activity. Of the gingipain-defective mutants, ΔThr378-His391 and ΔPhe377-His391 produced amounts of PG27 comparable to those produced by wild-type cells, suggesting that Thr378-Phe381 contains essential residues for the function of PG27. In contrast, ΔPhe381-His391, ΔAla380-His391, ΔLeu379-His391 and ΔArg376-His391 produced no detectable PG27. The defects of the ΔAla380-His391 mutant were suppressed by changing either Ala346 or Ala359 of PG27 to valine. Importantly, Ala346 and Ala359 are located close to Leu379 in the structural model of PG27. A359V compensated for the instability of PG27, but not the gingipain-defective phenotypes, of other deletion mutants tested, suggesting that Ala380 and Phe381 of PG27 are important for the stability of PG27. Lastly, we found that the C-terminal region of PG27 may be located in the periplasm. Taken together, these findings fit well with a predicted β-barrel structure model for PG27, and show that strand S14 is important for its function.
authors: Department of Microbiology, Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan.
authors: I
authors: I
authors: Ishiguro
authors: K
authors: K
authors: Saiki
authors: K
authors: K
authors: Konishi
mesh_terms: D000215:Acylation; D018829:Adhesins, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D000080867:Gingipain Cysteine Endopeptidases; D008070:Lipopolysaccharides / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000235:genetics*; D016966:Porphyromonas gingivalis / Q000201:enzymology* / Q000235:genetics*; D017434:Protein Structure, Tertiary; D017384:Sequence Deletion; D013489:Suppression, Genetic; D037521:Virulence Factors / Q000737:chemistry / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D000080867:Gingipain Cysteine Endopeptidases; D008070:Lipopolysaccharides; D026901:Membrane Transport Proteins; D037521:Virulence Factors; D003546:Cysteine Endopeptidases