title: Wiring of redox enzymes on three dimensional self-assembled molecular scaffold.
abstract: The integration of biological molecules and nanoscale components provides a fertile basis for the construction of hybrid materials of synergic properties and functions. Stable protein 1 (SP1), a highly stable ring shaped protein, was recently used to display different functional domains, to bind nanoparticles (NPs), and to spontaneously form two and three-dimensional structures. Here we show an approach to wire redox enzymes on this self-assembled protein-nanoparticle hybrid. Those hybrids are genetically engineered SP1s, displaying glucose oxidase (GOx) enzymes tethered to the protein inner pore. Moreover, the Au-NP-protein hybrids self-assembled to multiple enzymatic layers on the surface. By wiring the redox enzymes to the electrode, we present an active structure for the bioelectrocatalytic oxidation of glucose. This system demonstrates for the first time a three-dimensional assembly of multiple catalytic modules on a protein scaffold with an efficient electrical wiring of the enzyme units on an electrode surface, thus implementing a hybrid electrically active unit for nanobioelectronic applications.
authors: Department of Chemistry and Drug Technology, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
authors: Marco
authors: M
authors: Frasconi
authors: Arnon
authors: A
authors: Heyman
authors: Izhar
authors: I
authors: Medalsy
authors: Danny
authors: D
authors: Porath
authors: Franco
authors: F
authors: Mazzei
authors: Oded
authors: O
authors: Shoseyov
mesh_terms: D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008958:Models, Molecular; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D006046:Gold; D005949:Glucose Oxidase