title: Comparative proteomic analysis of mesenchymal stem cells derived from human bone marrow, umbilical cord and placenta: implication in the migration.
abstract: Umbilical cord (UC) and placenta (P) have been suggested as alternatives to bone marrow (BM) as sources of mesenchymal stem cells (MSC) for cell therapy, with both UC- and P-MSC possess immunophenotypic and functional characteristics similar to BM-MSC. However, under defined conditions, the migration capacity of BM- and P-MSC was found to be 5.9- and 3.2-folds higher than that of UC-MSC, respectively. By the use of 2-DE and combined MS and MS/MS analysis, six differentially expressed proteins were identified among these MSC samples, with five of them known to be involved in cell migration as migration enhancing or inhibiting proteins. Interestingly, the expression levels of those proteins reflect perfectly the migration capacity of corresponding MSC, which is also proved by in vitro overexpression and silencing techniques. Our study indicates that a bunch of migration-related proteins are pivotal in governing the migration capacity of MSC.
authors: Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, People's Republic of China.
authors: Guo
authors: G
authors: Li
authors: Xiao-Ai
authors: XA
authors: Zhang
authors: Hua
authors: H
authors: Wang
authors: Xin
authors: X
authors: Wang
authors: Chun-Ling
authors: CL
authors: Meng
authors: Chu-Yan
authors: CY
authors: Chan
authors: David Tai Wai
authors: DT
authors: Yew
authors: Kam Sze
authors: KS
authors: Tsang
authors: Karen
authors: K
authors: Li
authors: Sau-Na
authors: SN
authors: Tsai
authors: Sai-Ming
authors: SM
authors: Ngai
authors: Zhong Chao
authors: ZC
authors: Han
authors: Marie Chia-Mi
authors: MC
authors: Lin
authors: Ming-Liang
authors: ML
authors: He
authors: Hsiang-Fu
authors: HF
authors: Kung
mesh_terms: D001854:Bone Marrow Cells / Q000737:chemistry* / Q000502:physiology; D002401:Cathepsin B / Q000032:analysis / Q000502:physiology; D002465:Cell Movement; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000502:physiology; D010920:Placenta / Q000737:chemistry*; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis / Q000502:physiology; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D014470:Umbilical Cord / Q000737:chemistry*
chemical_list: D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human; C533912:CTSB protein, human; D002401:Cathepsin B