title: [Effect of HPV 16 E5 on E6 and E7 gene induced human immortalized oral epithelial cell].
abstract: OBJECTIVE
  To investigate the influence of HPV16 E5 on E6 and E7 gene in human oral immortalized epithelial cells (HIOEC).
  
  
  METHODS
  The pLEGFP-E5 was transferred into HIOEC cell; Deletion mutation expression vectors were constructed and transferred into HIOEC; RT-PCR was used to detect the gene expression in HIOEC; The expression of E6 and E7 mRNA was detected by real time-PCR;Cell proliferation of HIOEC was evaluated by MTT. All statistical analysis was performed by using SPSS 13.0 software package.
  
  
  RESULTS
  The deletion mutation expression vector of E5 was successfully constructed and applied for further experiments; E5 and the deletion mutation genes were transferred into HIOEC successfully; HPV16 E5 promoted cell proliferation of HIOEC and made the increased expression raised of E6 and E7 genes; However, the deletion mutation had no significant effect on HIOEC.
  
  
  CONCLUSIONS
  HPV16 E5 is transferred into HIOEC successfully. E5 may promote cell proliferation and upregulate the mRNA expression of E6 and E7 genes.
authors: Department of Stomatology, the Third Affiliated Hospital of Anhui Medical University. Hefei 230001, Anhui Province, China. hxx198402@sina.cn
authors: Xiao-xiao
authors: XX
authors: Hou
authors: Tong
authors: T
authors: Jiang
authors: Xin-quan
authors: XQ
authors: Jiang
authors: Chuan-jun
authors: CJ
authors: Chen
mesh_terms: D049109:Cell Proliferation; D004847:Epithelial Cells; D052162:Human papillomavirus 16; D006801:Humans; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins
chemical_list: D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins