title: Effect of alternate day feeding strategy of sub-optimal protein level on haemato-biochemical responses in Labeo rohita (Hamilton) juveniles.
abstract: A feeding trial was conducted for 60 days to delineate the effect of alternate day feeding strategy of sub-optimal protein level on haematological parameters, serum parameters and phagocyte respiratory burst activity (NBT) in Labeo rohita juveniles. One hundred and thirty-five fingerlings (1.87 ± 0.01-2.26 ± 0.05 g) were distributed in triplicate groups of each treatment, and fish were fed at 5% body weight daily. Three experimental isocaloric (401.32-410.28 kcal/100 g) diets of 30%, 25%, and 20% crude protein designated as diet A, diet B, and diet C respectively, were prepared, using locally available feed ingredients. Three different feeding schedules of normal protein diet continuously (diet A-30%), alternate feeding of 1-day diet A followed by 1-day diet B (1A/1B) and alternate feeding of 1-day diet A followed by 1-day diet C (1A/1C) were tested. The total erythrocyte count and haemoglobin content was significantly (p < 0.05) enhanced in the group T1 fed (1A/1B), and the lowest count was recorded in the group T2 fed (1A/1C). Total leucocyte counts, total serum protein, and serum globulin were higher in the group T1 fed (1A/1B) and lower in the group T2 fed (1A/1C) as compare to control. The respiratory burst activity (NBT) of blood phagocytes and serum A-to-G ratio was recorded significantly difference in among the treatment groups. Based on the results of the present study, it is concluded that alternate feeding of 1-day diet A followed by 1-day diet B (1A/1B) is equally effective and promote the immunity in Labeo rohita juveniles.
authors: Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, India. kumarpankajfnb@gmail.com
authors: P
authors: P
authors: Kumar
authors: K K
authors: KK
authors: Jain
authors: S
authors: S
authors: Munilkumar
authors: N P
authors: NP
authors: Sahu
authors: G M
authors: GM
authors: Siddaiah
authors: H
authors: H
authors: Saha
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000822:Animal Husbandry; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose; D003530:Cyprinidae / Q000097:blood / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000032:analysis / Q000494:pharmacology*; D016897:Respiratory Burst; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D004044:Dietary Proteins