title: Enhanced production of nukacin D13E in Lactococcus lactis NZ9000 by the additional expression of immunity genes.
abstract: Nukacin D13E (D13E) is a variant of type-A(II) lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. D13E exhibited a twofold higher specific antimicrobial activity than nukacin ISK-1 against a number of Gram-positive bacteria. We previously reported the heterologous production of D13E in Lactococcus lactis NZ9000 under the control of nisin-controlled gene expression system. In this study, we demonstrated enhanced production of D13E by the additional expression of immunity genes, nukFEG. The nukacin ISK-1 immunity, conferred by the ABC transporter complex, NukFEG, and the lantibiotic-binding protein, NukH, was not overwhelmed by D13E. The additional NukFEG resulted in a fourfold increase in the immunity level of the strain and a 5.2-fold increase in D13E production. The additional NukFEGH-expressing strain with the highest D13E immunity showed reduced level of production. Further improvement in D13E production was achieved by using pH-controlled batch fermentation.
authors: Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
authors: Tijo Varghese
authors: TV
authors: Puramattathu
authors: Mohammad R
authors: MR
authors: Islam
authors: Mami
authors: M
authors: Nishie
authors: Sae
authors: S
authors: Yanagihara
authors: Jun-ichi
authors: J
authors: Nagao
authors: Ken-ichi
authors: K
authors: Okuda
authors: Takeshi
authors: T
authors: Zendo
authors: Jiro
authors: J
authors: Nakayama
authors: Kenji
authors: K
authors: Sonomoto
mesh_terms: D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D024881:Drug Resistance, Bacterial; D015870:Gene Expression; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D020125:Mutation, Missense; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001430:Bacteriocins; D026901:Membrane Transport Proteins; D050505:Mutant Proteins; D011994:Recombinant Proteins; C421186:nukacin ISK-1