title: Hemin/G-quadruplexes as DNAzymes for the fluorescent detection of DNA, aptamer-thrombin complexes, and probing the activity of glucose oxidase.
abstract: Hemin/G-quadruplex catalyzes the H(2)O(2)-mediated oxidation of Amplex Red to the fluorescent product resorufin. This process is implemented to develop hairpin nucleic acid structures for the detection of DNA, to probe the catalytic activity of glucose oxidase, to use the thrombin-aptamer complex as a catalytic readout structure, and to quantitatively analyze telomere chain composition.
authors: Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
authors: Eyal
authors: E
authors: Golub
authors: Ronit
authors: R
authors: Freeman
authors: Angelica
authors: A
authors: Niazov
authors: Itamar
authors: I
authors: Willner
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis*; D052158:Aptamers, Peptide / Q000032:analysis; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes; D054856:G-Quadruplexes; D005949:Glucose Oxidase / Q000378:metabolism*; D006427:Hemin / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry; D010078:Oxazines / Q000138:chemical synthesis / Q000378:metabolism; D010084:Oxidation-Reduction; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D052158:Aptamers, Peptide; D021881:DNA, Catalytic; D005456:Fluorescent Dyes; D010078:Oxazines; C470430:Amplex Red; C081676:thrombin aptamer; C014180:resorufin; D006427:Hemin; D004247:DNA; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D013917:Thrombin