title: [Comparative investigation by spectrofluorimetry and flow cytometry of plasma and inner mitochondrial membranes polarisation in smooth muscle cell using potential-sensitive probe DiOC6(3)].
abstract: Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3'-dihexyloxacarbocyanine [DiOC6(3)] has been demonstrated. The obtained results confirm the use of DiOC6(3) for studying the influence of effectors on transmembrane potentials of intact cell compartments.
authors: G V
authors: GV
authors: Danylovich
authors: Iu V
authors: IuV
authors: Danylovich
authors: V F
authors: VF
authors: Gorchev
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002232:Carbocyanines / Q000032:analysis; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D007476:Ionophores / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D019810:Sodium Azide / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014599:Uterus / Q000166:cytology / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D002232:Carbocyanines; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007476:Ionophores; D014634:Valinomycin; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D010042:Ouabain; D019810:Sodium Azide; D002118:Calcium