title: A 5-microRNA signature for lung squamous cell carcinoma diagnosis and hsa-miR-31 for prognosis.
abstract: OBJECTIVE
  Recent studies have suggested that microRNA biomarkers could be useful for stratifying lung cancer subtypes, but microRNA signatures varied between different populations. Squamous cell carcinoma (SCC) is one major subtype of lung cancer that urgently needs biomarkers to aid patient management. Here, we undertook the first comprehensive investigation on microRNA in Chinese SCC patients.
  
  
  METHODS
  MicroRNA expression was measured in cancerous and noncancerous tissue pairs strictly collected from Chinese SCC patients (stages I-III), who had not been treated with chemotherapy or radiotherapy prior to surgery. The molecular targets of proposed microRNA were further examined.
  
  
  RESULTS
  We identified a 5-microRNA classifier (hsa-miR-210, hsa-miR-182, hsa-miR-486-5p, hsa-miR-30a, and hsa-miR-140-3p) that could distinguish SCC from normal lung tissues. The classifier had an accuracy of 94.1% in a training cohort (34 patients) and 96.2% in a test cohort (26 patients). We also showed that high expression of hsa-miR-31 was associated with poor survival in these 60 SCC patients by Kaplan-Meier analysis (P = 0.007), by univariate Cox analysis (P = 0.011), and by multivariate Cox analysis (P = 0.011). This association was independently validated in a separate cohort of 88 SCC patients (P = 0.008, 0.011, and 0.003 in Kaplan-Meier analysis, univariate Cox analysis, and multivariate Cox analysis, respectively). We then determined that the tumor suppressor DICER1 is a target of hsa-miR-31. Expression of hsa-miR-31 in a human lung cancer cell line repressed DICER1 activity but not PPP2R2A or LATS2.
  
  
  CONCLUSIONS
  Our results identified a new diagnostic microRNA classifier for SCC among Chinese patients and a new prognostic biomarker, hsa-miR-31.
authors: Department of Thoracic Surgery, Cancer Hospital and Institute, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, P.R. China.
authors: Xiaogang
authors: X
authors: Tan
authors: Wenyan
authors: W
authors: Qin
authors: Liang
authors: L
authors: Zhang
authors: Jie
authors: J
authors: Hang
authors: Baozhong
authors: B
authors: Li
authors: Cuiyan
authors: C
authors: Zhang
authors: Junting
authors: J
authors: Wan
authors: Fang
authors: F
authors: Zhou
authors: Kang
authors: K
authors: Shao
authors: Yimin
authors: Y
authors: Sun
authors: Jianping
authors: J
authors: Wu
authors: Xun
authors: X
authors: Zhang
authors: Bin
authors: B
authors: Qiu
authors: Ning
authors: N
authors: Li
authors: Susheng
authors: S
authors: Shi
authors: Xiaoli
authors: X
authors: Feng
authors: Shouhua
authors: S
authors: Zhao
authors: Zhen
authors: Z
authors: Wang
authors: Xiaohong
authors: X
authors: Zhao
authors: Zhaoli
authors: Z
authors: Chen
authors: Keith
authors: K
authors: Mitchelson
authors: Jing
authors: J
authors: Cheng
authors: Yong
authors: Y
authors: Guo
authors: Jie
authors: J
authors: He
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D053487:DEAD-box RNA Helicases / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D054648:Protein Phosphatase 2 / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D043244:Ribonuclease III / Q000096:biosynthesis / Q000235:genetics; D015996:Survival Rate; D014162:Transfection; D025521:Tumor Suppressor Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: C534612:MIRN31 microRNA, human; D035683:MicroRNAs; C512974:PPP2R2A protein, human; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases; C408087:DICER1 protein, human; D043244:Ribonuclease III; D054648:Protein Phosphatase 2; D053487:DEAD-box RNA Helicases