title: [Impact of ishemia-reperfusion injury on long survival rate in intestinal transplantation in rats].
abstract: BACKGROUND
  The intestine is a highly sensitive tissue to ischemia-reperfusion (IR) injury that will early respond increasing its permeability. Later this response is translated in morphologic and histological changes that reveal the degree of damage. The heterotopic intestinal transplantation model in rats allows to evaluate the evolution of intestinal tissue injury after ischemia-reperfusion without affecting the long survival rate.
  
  
  OBJECTIVE
  The aim of this paper is to establish a relationship between the ischemic reperfusion injury with the long-term survival
  
  
  METHODS
  Ten intestinal transplants were analyzed in adult, Wistar, inbred, male rats. Light microscopical examination was performed on intestine graft: 1) immediately post-dissection, 2) at the end of cold isquemia, 3) 30 min, 4) 48hs and 5) 5 days post-transplant procedure, respectively. Biopsies were reported according to Park's classification and extension of staining using immunohistochemestry to malondialdehyde (MDA) products.
  
  
  RESULTS
  The Park's classification indexes reported in samples were 1) 0,57 +/- 1,13 (N=10); 2) 2,71 +/- 1,25 (N=10); 3) 4,14 +/- 0,89 (N=10); 4) 1,0 +/- 0,81 (N=7); 5) 0 (N=7). The highest levels of immunohistochemical detection of MDA were observed thirty minutes post-reperfusion (extension of staining between 51% to 75%). Three animals died when they were sampled at 48 hours, and the biopsies had Park's classification > or = 4 at 30 minutes post-reperfusion and endotoxemic signology.
  
  
  CONCLUSIONS
  The highest degree of mucosal damage was observed immediately post-reperfusion. At 48hs the graft tended to be normalized Failure to repair the immediately I-R injury signficantly affects the long term survival.
authors: Instituto de Trasplante Multiorgánico, Fundación Favaloro, Buenos Aires, Argentina. nlausada@gmail.com
authors: Natalia
authors: N
authors: Lausada
authors: Pablo
authors: P
authors: Stringa
authors: Ana
authors: A
authors: Cabanne
authors: Diego
authors: D
authors: Ramisch
authors: Mariana
authors: M
authors: Machuca
authors: Flavio
authors: F
authors: Galvao
authors: Silvia
authors: S
authors: Coronato
authors: Jorge Clemente
authors: JC
authors: Raimondi
authors: Gabriel
authors: G
authors: Gondolesi
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007422:Intestines / Q000098:blood supply / Q000473:pathology / Q000637:transplantation*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000401:mortality* / Q000473:pathology; D012720:Severity of Illness Index; D015996:Survival Rate; D013997:Time Factors