title: Glomerular injury induced in mice by intraperitoneal injection of Shiga-like toxins.
abstract: OBJECTIVE
  Shiga-like toxins I and II (Stx1 and Stx2) play an important role in the pathogenesis of renal disease by causing renal microvascular injury. A murine model was used to study glomerular lesions produced by Stx1 and Stx2.
  
  
  METHODS
  Swiss albino mice of the Rockefeller strain were inoculated intraperitoneally with LD(50) doses of endotoxin-free Stx1 of Stx2 and observed for signs of disease. Samples of renal cortical tissue from mice were examined with the electron microscope.
  
  
  RESULTS
  the mice developed systemic and neurological symptoms including hind limb paralysis and generalised convulsions. Renal arteriolar damage and glomerular endothelial cytoplasmic swelling, vacuolation, lysis and intravascular coagulation were present and resembled the microangiopathy seen in renal biopsies from patients.
  
  
  CONCLUSIONS
  these experiments establish the role of Stx1 and Stx2 in glomerular vascular injury and provide a model for studying the pathogenesis of Shiga-like toxin related microangiopathy.
authors: Department of Anatomy, Christian Medical College and Hospital, Vellore, India.
authors: R
authors: R
authors: Koshi
authors: A B
authors: AB
authors: Pulimood
authors: A
authors: A
authors: Date
authors: G
authors: G
authors: Kang
authors: D
authors: D
authors: Acheson
authors: M M
authors: MM
authors: Mathan
mesh_terms: D000818:Animals; D007274:Injections, Intraperitoneal; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D011500:Protein Synthesis Inhibitors / Q000633:toxicity; D022622:Shiga Toxin 1 / Q000633:toxicity*; D022641:Shiga Toxin 2 / Q000633:toxicity*
chemical_list: D011500:Protein Synthesis Inhibitors; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2