title: Evaluation of the automated collection and extended storage of apheresis platelets in additive solution.
abstract: BACKGROUND
  Collecting apheresis platelets (PLTs) into additive solution has many potential benefits. The new Trima software (Version 6.0, CaridianBCT) allows automated addition of PLT additive solution (PAS) after collection, compared to Trima Version 5.1, which only collects PLTs into plasma. The aim of this study was to compare PLT quality during extended storage, after collection with the different Trima systems.
  
  
  METHODS
  Apheresis PLTs were collected using both Trima Accel apheresis systems. The test PLT units (n = 12) were collected using the new Trima Version 6.0 into PLT AS (PAS-IIIM), while the control units (n = 8) were collected into autologous plasma using Trima Version 5.1. All units were stored for 9 days, and in vitro cell quality variables were evaluated during this time.
  
  
  RESULTS
  PLTs collected in PAS-IIIM maintained a stable pH between 7.2 and 7.4, whereas plasma-stored apheresis units exhibited significantly increased acidity during storage, due to lactate accumulation and bicarbonate exhaustion. Plasma-stored PLTs also demonstrated a more rapid consumption of glucose. However, there was little difference in PLT activation or cytokine secretion between PAS-IIIM and control PLTs.
  
  
  CONCLUSIONS
  These data indicate that apheresis PLT concentrates collected in PAS-IIIM, using Trima Version 6.0 software, maintained acceptable PLT metabolic and cellular characteristics until Day 9 of storage.
authors: Research and Development, Australian Red Cross Blood Service, Sydney, Australia.
authors: Lacey
authors: L
authors: Johnson
authors: Kelly M
authors: KM
authors: Winter
authors: Tanja
authors: T
authors: Hartkopf-Theis
authors: Samantha
authors: S
authors: Reid
authors: Matthew
authors: M
authors: Kwok
authors: Denese C
authors: DC
authors: Marks
mesh_terms: D000085:Acetates / Q000378:metabolism; D001639:Bicarbonates / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016207:Cytokines / Q000378:metabolism; D005947:Glucose / Q000493:pharmacokinetics; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects / Q000502:physiology; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards; D012984:Software; D012996:Solutions / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000085:Acetates; D001639:Bicarbonates; D016207:Cytokines; D012996:Solutions; D019344:Lactic Acid; D005947:Glucose