title: Characterization of Candida sp. NY7122, a novel pentose-fermenting soil yeast.
abstract: Yeasts that ferment both hexose and pentose are important for cost-effective ethanol production. We found that the soil yeast strain NY7122 isolated from a blueberry field in Tsukuba (East Japan) could ferment both hexose and pentose (D-xylose and L-arabinose). NY7122 was closely related to Candida subhashii on the basis of the results of molecular identification using the sequence in the D1/D2 domains of 26S rDNA and 5.8S-internal transcribed spacer region. NY7122 produced at least 7.40 and 3.86 g l⁻¹ ethanol from 20 g l⁻¹ D-xylose and L-arabinose within 24 h. NY7122 could produce ethanol from pentose and hexose sugars at 37°C. The highest ethanol productivity of NY7122 was achieved under a low pH condition (pH 3.5). Fermentation of mixed sugars (50 g l⁻¹ glucose, 20 g l⁻¹ D-xylose, and 10 g l⁻¹ L-arabinose) resulted in a maximum ethanol concentration of 27.3 g l⁻¹ for the NY7122 strain versus 25.1 g l⁻¹ for Scheffersomyces stipitis. This is the first study to report that Candida sp. NY7122 from a soil environment could produce ethanol from both D-xylose and L-arabinose.
authors: Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors: Itsuki
authors: I
authors: Watanabe
authors: Akira
authors: A
authors: Ando
authors: Toshihide
authors: T
authors: Nakamura
mesh_terms: D000818:Animals; D001089:Arabinose / Q000378:metabolism; D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007564:Japan; D010429:Pentoses / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000378:metabolism; D012988:Soil Microbiology; D014994:Xylose / Q000378:metabolism
chemical_list: D056804:Biofuels; D010429:Pentoses; D000431:Ethanol; D014994:Xylose; D001089:Arabinose; D005947:Glucose