title: Recent increase of genetic diversity in Plasmodium vivax population in the Republic of Korea.
abstract: BACKGROUND
  The reemergence of Plasmodium vivax in South Korea since 1993 represents a serious public health concern. Despite the importance in understanding genetic diversity for control strategies, however, studies remain inconclusive with the general premise that due to low rate of malaria transmission, there is generally low genetic diversity with very few strains involved. In this study, the genetic diversity and population structure of P. vivax in South Korea were explored by analysing microsatellite polymorphism.
  
  
  METHODS
  Sequences for 13 microsatellite loci distributed across the twelve chromosomes of P. vivax were obtained from 58 South Korean isolates collected during two sampling periods, namely 1997-2000 and 2007. The sequences were used for the analysis of expected heterozygosity and multilocus genotype diversity. Population structure was evaluated using STRUCTURE version 2.3.2. Linkage disequilibrium was also analysed to investigate the extent of outbreeding in the P. vivax population.
  
  
  RESULTS
  Mean expected heterozygosity significantly increased from 0.382 in 1997-2000 to 0.545 in 2007 (P < 0.05). The number of multilocus genotypes was 7 and 27; and genotype diversity was statistically significant (P < 0.01) at 0.661 and 0.995 in 1997-2000 and 2007, respectively. Analysis by STRUCTURE showed a more complex population structure in 2007 than in 1997-2000. Linkage disequilibrium between 13 microsatellites, although significant in both time points, was notably lower in 2007.
  
  
  CONCLUSIONS
  The present microsatellite analysis clearly showed recent increase of genetic diversity and recent relaxation of the strong population structure observed in 1997-2000. These results suggest that multiple genotypes not present previously recently migrated into South Korea, accompanied by substantial outbreeding between different genotypes.
authors: Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
authors: Hajime
authors: H
authors: Honma
authors: Jung-Yeon
authors: JY
authors: Kim
authors: Nirianne M Q
authors: NM
authors: Palacpac
authors: Toshihiro
authors: T
authors: Mita
authors: Wonja
authors: W
authors: Lee
authors: Toshihiro
authors: T
authors: Horii
authors: Kazuyuki
authors: K
authors: Tanabe
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D006801:Humans; D018895:Microsatellite Repeats; D010966:Plasmodium vivax / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D056910:Republic of Korea
chemical_list: D016054:DNA, Protozoan