title: Youngia denticulata protects against oxidative damage induced by tert-butylhydroperoxide in HepG2 cells.
abstract: Improvement of liver function is one of the most popular commercial health claims of functional foods in Asian countries, including Korea. After examining the potential of several traditional Korean wild vegetables for enhancing liver function, we found that Youngia denticulata Kitam. has strong hepatoprotective effects against oxidative stress induced by tert-butylhydroperoxide (t-BHP). We are the first to report that the extract and ethyl acetate fractions of Y. denticulata have radical scavenging activities and inhibit oxidative stress-induced cell death and DNA damage in HepG2 cells. The extract and ethyl acetate fractions significantly decreased cellular reactive oxygen species production and apoptosis induced by t-BHP in HepG2 cells. In addition, they prevented the depletion of cellular glutathione, which is an important defense molecule against oxidizing xenobiotics. Chlorogenic acid and 3,5-dicaffeoylquinic acid were found to be major active components responsible for the activity of Y. denticulata and could serve as marker compounds for standardization. These data suggest that Y. denticulata could be promoted as a potential antioxidative functional food candidate, particularly for hepatoprotection against oxidative stress.
authors: Functional Food Center, Korea Institute of Science and Technology, Gangneung Institute, Gangwon-do, Korea.
authors: Kyungsu
authors: K
authors: Kang
authors: Eun Hye
authors: EH
authors: Jho
authors: Hee Ju
authors: HJ
authors: Lee
authors: Sarangerel
authors: S
authors: Oidovsambuu
authors: Ji Ho
authors: JH
authors: Yun
authors: Chul Young
authors: CY
authors: Kim
authors: Ji-Hye
authors: JH
authors: Yoo
authors: Young-Jin
authors: YJ
authors: Kim
authors: Jong Hwan
authors: JH
authors: Kim
authors: Soo Young
authors: SY
authors: Ahn
authors: Chu Won
authors: CW
authors: Nho
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D020122:tert-Butylhydroperoxide; D005978:Glutathione; C100434:3,5-dicaffeoylquinic acid