title: Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects.
abstract: BACKGROUND
  The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser).
  
  
  METHODS
  Biopsy of the first and second PB (PB1 and PB2) was performed by mechanical- or laser-assisted biopsy in two different IVF centres. PBs were separately amplified by whole genome amplification.
  
  
  RESULTS
  The method of biopsy, mechanical or laser had no influence on the proportion of successfully biopsied oocytes. Especially, for the PB2, the timing of biopsy after ICSI was directly correlated to amplification efficiency.
  
  
  CONCLUSIONS
  Special care has to be taken with respect to the timing of biopsy of the PB2. Mechanical- and laser-assisted biopsy give the same performance in terms of diagnostic efficiency.
authors: Department of Reproductive Medicine, SISMER, Via Mazzini 12, Bologna 40138, Italy. cristina.magli@sismer.it
authors: M Cristina
authors: MC
authors: Magli
authors: Markus
authors: M
authors: Montag
authors: Maria
authors: M
authors: Köster
authors: Luigi
authors: L
authors: Muzi
authors: Joep
authors: J
authors: Geraedts
authors: John
authors: J
authors: Collins
authors: Veerle
authors: V
authors: Goossens
authors: Alan H
authors: AH
authors: Handyside
authors: Joyce
authors: J
authors: Harper
authors: Sjoerd
authors: S
authors: Repping
authors: Andreas
authors: A
authors: Schmutzler
authors: Katerina
authors: K
authors: Vesela
authors: Luca
authors: L
authors: Gianaroli
mesh_terms: D000782:Aneuploidy; D001706:Biopsy / Q000379:methods; D002875:Chromosomes / Q000648:ultrastructure*; D055028:Comparative Genomic Hybridization / Q000379:methods*; D054885:Cumulus Cells / Q000166:cytology; D004247:DNA / Q000235:genetics; D005260:Female; D005821:Genetic Techniques; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology*; D059705:Polar Bodies / Q000166:cytology*; D027724:Reproductive Techniques, Assisted; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
chemical_list: D004247:DNA