title: Comparison of human blood concentrations of collectin kidney 1 and mannan-binding lectin.
abstract: Mannan-binding lectin (MBL) was first discovered as a collectin in animal blood, and was shown to have such unique characteristics as a collage-like domain and a carbohydrate recognition domain. We recently identified human collectin kidney 1 (CL-K1, COLEC11) from a human kidney cDNA library. To quantitate the CL-K1 concentration in blood, we developed several polyclonal and monoclonal antibodies using recombinant human CL-K1 in CHO cells and the CL-K1 fragment in Escherichia coli. Using these antibodies, we established a sandwich enzyme-linked immunosorbent assay (ELISA) system. The concentration of CL-K1 in human plasma was 0.34 ± 0.13 µg/ml and that in MBL was 1.72 ± 1.51 µg/ml. Concentrations of MBL are often low due to its single nucleotide polymorphisms (SNPs) which seem to be related to an opsonic defect. However, no low concentrations of CL-K1 were observed on testing over two hundred blood samples. We also found that the blood concentration of CL-K1 was not dependent on gender or age and did not correlate completely with that of MBL. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of CL-K1 in humans.
authors: Department of Microbiology and Immunochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510, Japan.
authors: Takayuki
authors: T
authors: Yoshizaki
authors: Katsuki
authors: K
authors: Ohtani
authors: Wataru
authors: W
authors: Motomura
authors: Seong-Jae
authors: SJ
authors: Jang
authors: Ken-ichiro
authors: K
authors: Mori
authors: Noritoshi
authors: N
authors: Kitamoto
authors: Itsuro
authors: I
authors: Yoshida
authors: Yasuhiko
authors: Y
authors: Suzuki
authors: Nobutaka
authors: N
authors: Wakamiya
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D015153:Blotting, Western; D016466:CHO Cells; D037222:Collectins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D006224:Cricetinae; D003412:Cricetulus; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood* / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D012016:Reference Values; D055815:Young Adult
chemical_list: D000906:Antibodies; C518521:Colec11 protein, human; D037222:Collectins; D037601:Mannose-Binding Lectin; D011994:Recombinant Proteins