title: A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.
abstract: Acid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach (pH 1-3). The client proteins of these chaperones remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions. We developed a genetically encoded, highly efficient protein photocrosslinking probe, which enabled us to profile the in vivo substrates of a major acid-protection chaperone, HdeA, in Escherichia coli periplasm. Among the identified HdeA client proteins, the periplasmic chaperones DegP and SurA were initially found to be protected by HdeA at a low pH, but they subsequently facilitated the HdeA-mediated acid recovery of other client proteins. This unique, ATP-independent chaperone cooperation in the ATP-deprived E. coli periplasm may support the acid resistance of enteric bacteria. The crosslinker would be valuable in unveiling the physiological interaction partners of any given protein and thus their functions under normal and stress conditions.
authors: State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.
authors: Meng
authors: M
authors: Zhang
authors: Shixian
authors: S
authors: Lin
authors: Xinwen
authors: X
authors: Song
authors: Jun
authors: J
authors: Liu
authors: Ye
authors: Y
authors: Fu
authors: Xi
authors: X
authors: Ge
authors: Xinmiao
authors: X
authors: Fu
authors: Zengyi
authors: Z
authors: Chang
authors: Peng R
authors: PR
authors: Chen
mesh_terms: D000143:Acids / Q000737:chemistry / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: D000143:Acids; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; C084501:hdeA protein, E coli