title: Polymerase chain reaction-based identification of clinically relevant Pasteurellaceae isolated from cats and dogs in Poland.
abstract: A set of polymerase chain reaction (PCR) assays for identification of the most important Pasteurellaceae species encountered in cats and dogs were developed. Primers for Pasteurella multocida were designed to detect a fragment of the kmt, a gene encoding the outer-membrane protein. Primers specific to Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis were based on the manganese-dependent superoxide dismutase gene (sodA) and those specific to [Haemophilus] haemoglobinophilus on species-specific sequences of the 16S ribosomal RNA gene. All the primers were tested on respective reference and control strains and applied to the identification of 47 canine and feline field isolates of Pasteurellaceae. The PCR assays were shown to be species specific, providing a valuable supplement to phenotypic identification of species within this group of bacteria.
authors: Department of Pathology, University of Environmental and Life Sciences, Norwida St. 31, 50-375 Wroclaw, Poland. jkrol4@o2.pl
authors: Jaroslaw
authors: J
authors: Król
authors: Jacek
authors: J
authors: Bania
authors: Magdalena
authors: M
authors: Florek
authors: Aleksandra
authors: A
authors: Pliszczak-Król
authors: Zdzislaw
authors: Z
authors: Staroniewicz
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000382:microbiology; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis* / Q000382:microbiology; D004285:Dogs; D010325:Pasteurella / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary; D015661:Pasteurellaceae / Q000302:isolation & purification; D016871:Pasteurellaceae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D010641:Phenotype; D011044:Poland; D016133:Polymerase Chain Reaction / Q000662:veterinary*