title: A genome-wide RNA interference screen uncovers two p24 proteins as regulators of Wingless secretion.
abstract: Wnt proteins are secreted, lipid-modified glycoproteins that control animal development and adult tissue homeostasis. Secretion of Wnt proteins is at least partly regulated by a dedicated machinery. Here, we report a genome-wide RNA interference screen for genes involved in the secretion of Wingless (Wg), a Drosophila Wnt. We identify three new genes required for Wg secretion. Of these, Emp24 and Eclair are required for proper export of Wg from the endoplasmic reticulum (ER). We propose that Emp24 and Eca act as specific cargo receptors for Wg to concentrate it in forming vesicles at sites of ER export.
authors: Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland.
authors: Fillip
authors: F
authors: Port
authors: George
authors: G
authors: Hausmann
authors: Konrad
authors: K
authors: Basler
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017344:Genes, Insect / Q000235:genetics; D056726:Genetic Association Studies; D005820:Genetic Testing; D049750:Genome, Insect / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D034622:RNA Interference; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D051155:Wnt1 Protein / Q000378:metabolism*
chemical_list: C570399:CHOp24 protein, Drosophila; D029721:Drosophila Proteins; D026901:Membrane Transport Proteins; D033921:Vesicular Transport Proteins; D051155:Wnt1 Protein; C499967:eca protein, Drosophila; C074622:wg protein, Drosophila