title: Serum metabolomics reveals higher levels of polyunsaturated fatty acids in lepromatous leprosy: potential markers for susceptibility and pathogenesis.
abstract: BACKGROUND
  Leprosy is a disease of the skin and peripheral nervous system caused by the obligate intracellular bacterium Mycobacterium leprae. The clinical presentations of leprosy are spectral, with the severity of disease determined by the balance between the cellular and humoral immune response of the host. The exact mechanisms that facilitate disease susceptibility, onset and progression to certain clinical phenotypes are presently unclear. Various studies have examined lipid metabolism in leprosy, but there has been limited work using whole metabolite profiles to distinguish the clinical forms of leprosy.
  
  
  RESULTS
  In this study we adopted a metabolomics approach using high mass accuracy ultrahigh pressure liquid chromatography mass spectrometry (UPLC-MS) to investigate the circulatory biomarkers in newly diagnosed untreated leprosy patients. Sera from patients having bacterial indices (BI) below 1 or above 4 were selected, subjected to UPLC-MS, and then analyzed for biomarkers which distinguish the polar presentations of leprosy. We found significant increases in the abundance of certain polyunsaturated fatty acids (PUFAs) and phospholipids in the high-BI patients, when contrasted with the levels in the low-BI patients. In particular, the median values of arachidonic acid (2-fold increase), eicosapentaenoic acid (2.6-fold increase) and docosahexaenoic acid (1.6-fold increase) were found to be greater in the high-BI patients.
  
  
  CONCLUSIONS
  Eicosapentaenoic acid and docosahexaenoic acid are known to exert anti-inflammatory properties, while arachidonic acid has been reported to have both pro- and anti-inflammatory activities. The observed increase in the levels of several lipids in high-BI patients may provide novel clues regarding the biological pathways involved in the immunomodulation of leprosy. Furthermore, these results may lead to the discovery of biomarkers that can be used to investigate susceptibility to infection, facilitate early diagnosis and monitor the progression of disease.
authors: Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.
authors: Reem
authors: R
authors: Al-Mubarak
authors: Jason
authors: J
authors: Vander Heiden
authors: Corey D
authors: CD
authors: Broeckling
authors: Marivic
authors: M
authors: Balagon
authors: Patrick J
authors: PJ
authors: Brennan
authors: Varalakshmi D
authors: VD
authors: Vissa
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D005260:Female; D006801:Humans; D015440:Leprosy, Lepromatous / Q000175:diagnosis* / Q000503:physiopathology*; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000472:pathogenicity*; D044967:Serum / Q000737:chemistry*
chemical_list: D015415:Biomarkers; D005231:Fatty Acids, Unsaturated