title: Novel strategy for microsphere-mediated DNA transfection.
abstract: A new approach for microsphere-mediated delivery of plasmid DNA has been developed and successfully evaluated. Basic molecular biology techniques were used to linearize and functionalize plasmid DNA by aminomodification, enabling efficient conjugation to carboxy-functionalized microspheres. A T cell hybridoma line was successfully transfected as determined by the efficient expression of a biologically relevant YFP fusion protein. Moreover, our data identified microsphere-mediated delivery of plasmid DNA as a noninvasive, nontoxic, and efficient gene delivery method with the potential to be applied to transfection-resistant, nondividing primary cells, including naïve T cells.
authors: Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom.
authors: Jessica G
authors: JG
authors: Borger
authors: Juan Manuel
authors: JM
authors: Cardenas-Maestre
authors: Rose
authors: R
authors: Zamoyska
authors: Rosario M
authors: RM
authors: Sanchez-Martin
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D006801:Humans; D006825:Hybridomas; D008863:Microspheres; D010957:Plasmids / Q000008:administration & dosage*; D013601:T-Lymphocytes / Q000378:metabolism; D014162:Transfection
chemical_list: D004247:DNA