title: The ameliorative effect of 23-hydroxytormentic acid isolated from Rubus coreanus on cisplatin-induced nephrotoxicity in rats.
abstract: Previously, the authors demonstrated that the triterpenoid glycoside niga-ichigoside F₁ (NIF₁) and its aglycone 23-hydroxytormentic acid (23-HTA) isolated from the unripe fruits of Rubus coreanus (Rosaceae) ameliorate cisplatin-induced toxicity in renal epithelial LLC-PK₁ cells. In the present study, the nephroprotective effects of NIF₁ and 23-HTA were investigated in Sprague-Dawley rats with acute renal injury induced by a single intraperitoneal (i.p.) injection of cisplatin (7 mg/kg). Pretreatment with 23-HTA (10 mg/kg/d, per os (p.o.)) significantly reduced cisplatin-induced elevations in blood urea nitrogen (BUN) and serum creatinine level, whereas NIF₁ (10 mg/kg, p.o.) slightly reduced these levels. In addition, pretreatment with 23-HTA prevented cisplatin-induced hydroxyl radical generation, malondialdehyde (MDA) production, glutathione (GSH) depletion, and cisplatin-induced changes in the activities of oxidant and antioxidant enzymes in rat renal tissues. In addition, histopathological examinations showed that 23-HTA pretreatment reduced cisplatin-induced acute tubular necrosis and histological changes. In contrast, NIF₁ was found to have a slight or no influence on cisplatin-induced oxidative enzymes and acute tubular necrosis. Taken together, these results suggest that protective effect of 23-HTA pretreatment on cisplatin-induced renal damage is associated with the attenuation of oxidative stress and the preservation of antioxidant enzymes.
authors: Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Korea.
authors: Se-Il
authors: SI
authors: Sohn
authors: Hong-Kun
authors: HK
authors: Rim
authors: Yang-Hee
authors: YH
authors: Kim
authors: Jung-Hye
authors: JH
authors: Choi
authors: Jae-Hoon
authors: JH
authors: Park
authors: Hee-Juhn
authors: HJ
authors: Park
authors: Jong-Won
authors: JW
authors: Choi
authors: Soo-Dong
authors: SD
authors: Kim
authors: Seo-Young
authors: SY
authors: Jeong
authors: Kyung-Tae
authors: KT
authors: Lee
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D001806:Blood Urea Nitrogen; D002945:Cisplatin / Q000633:toxicity*; D003404:Creatinine / Q000097:blood; D005978:Glutathione / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D018374:LLC-PK1 Cells; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: C511643:23-hydroxytormentic acid; D000970:Antineoplastic Agents; D000975:Antioxidants; D014315:Triterpenes; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D003404:Creatinine; D005978:Glutathione; D002945:Cisplatin