title: Protective effect of catechin in type I Gaucher disease cells by reducing endoplasmic reticulum stress.
abstract: Gaucher disease (GD) is the most common lysosomal storage disorder (LSD) and is divided into three phenotypes, I, II, and III. Type I is the most prevalent form and has its onset in adulthood. The degree of endoplasmic reticulum (ER) stress is one of the factors that determine GD severity. It has recently been reported that antioxidants reduce ER stress and apoptosis by scavenging the oxidants that cause oxidative stress. For this report, we investigated the possibility that catechin can act on type I GD patient cells to alleviate the pathogenic conditions of GD. We treated GD cells with catechin and examined the expression level of GRP78/BiP (an ER stress marker) by western blots and fluorescence microscopy, the proliferation rate of GD cells, and scratch-induced wound healing activity. Our results show that catechin reduces the expression level of GRP78/BiP, leads to cell proliferation rates of GD cells similar levels to normal cells, and improves wound healing activity. We conclude that catechin protects against ER stress in GD cells and catechin-mediated reductions in ER stress may be associated with enhanced cell survival.
authors: Department of Biotechnology, Hoseo University, Baebang, Asan, Chungnam, 336-795, Republic of Korea.
authors: Yea-Jin
authors: YJ
authors: Lee
authors: Sung-Jo
authors: SJ
authors: Kim
authors: Tae-Hwe
authors: TH
authors: Heo
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D019610:Cytoprotection; D004721:Endoplasmic Reticulum / Q000187:drug effects*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005776:Gaucher Disease / Q000378:metabolism / Q000473:pathology*; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors; D006801:Humans; D013312:Stress, Physiological / Q000187:drug effects*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000975:Antioxidants; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D002392:Catechin