title: The acute and long-term effects of Middle East sand particles on the rat airway following a single intratracheal instillation.
abstract: Military personnel deployed in the Middle East have emphasized concerns regarding high levels of dust generated from blowing desert sand and the movement of troops and equipment. Airborne particulate matter levels (PM(10); PM < 10 μm) in the region may exceed 1500 μg/m(3), significantly higher than the military exposure guideline (MEG) of 50 μg/m(3). Increases in PM(10) have been linked to a rise in incidences of asthma, obstructive pulmonary disease, lung cancer, and cardiovascular diseases. Male Sprague-Dawley rats received a single intratracheal (IT) instillation of 1, 5, or 10 mg of Middle East PM(10) collected at a military occupied site in Kuwait, silica (positive control), or titanium dioxide (TiO(2); negative control) suspended in 400 μl sterile saline, or saline alone (vehicle control). Twenty-four hours, 3 d, 7 d and 6 mo postexposure (n = 15/group), organs including lung were evaluated for histopathological changes and for particle contaminants. Bronchoalveolar fluid (BALF) was also analyzed for cellular and biochemical parameters, including cytokines and chemokines. Instillation of silica resulted in early, pronounced, sustained inflammation indicated by significant increases in levels of total protein and neutrophils, and activities of lactate dehydrogenase activity and β-glucuronidase activity. Lower magnitude and transient changes using the same markers were observed in animals exposed to TiO(2) and Middle East PM(10). The results suggest that for acute exposures, this Middle East PM(10) is a nuisance-type dust with relatively low toxicity. However, since average deployment of military personnel to the Middle East is 180 d with potential for multiple follow-on tours, chronic exposure studies are needed to fully understand the pulmonary effects associated with Middle East PM exposure.
authors: United States Naval Academy, Department of Chemistry, Annapolis, Maryland 21402, USA. Wilfong@usna.edu
authors: E R
authors: ER
authors: Wilfong
authors: M
authors: M
authors: Lyles
authors: R L
authors: RL
authors: Rietcheck
authors: D P
authors: DP
authors: Arfsten
authors: H J
authors: HJ
authors: Boeckman
authors: E W
authors: EW
authors: Johnson
authors: T L
authors: TL
authors: Doyle
authors: G D
authors: GD
authors: Chapman
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005966:Glucuronidase / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D007730:Kuwait; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D052638:Particulate Matter / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013995:Time; D014025:Titanium / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D052638:Particulate Matter; C009495:titanium dioxide; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase