title: RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif.
abstract: Homologous recombination (HR) reactions mediated by the RAD51 recombinase are essential for DNA and replication fork repair, genome stability, and tumor suppression. RAD51-associated protein 1 (RAD51AP1) is an important HR factor that associates with and stimulates the recombinase activity of RAD51. We have recently shown that RAD51AP1 also partners with the meiotic recombinase DMC1, displaying isoform-specific interactions with DMC1. Here, we have characterized the DMC1 interaction site in RAD51AP1 by a series of truncations and point mutations to uncover a highly conserved WVPP motif critical for DMC1 interaction but dispensable for RAD51 association. This RAD51AP1 motif is reminiscent of the FVPP motif in the tumor suppressor protein BRCA2 that mediates DMC1 interaction. These results further implicate RAD51AP1 in meiotic HR via RAD51 and DMC1.
authors: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors: Myun Hwa
authors: MH
authors: Dunlop
authors: Eloïse
authors: E
authors: Dray
authors: Weixing
authors: W
authors: Zhao
authors: Miaw-Sheue
authors: MS
authors: Tsai
authors: Claudia
authors: C
authors: Wiese
authors: David
authors: D
authors: Schild
authors: Patrick
authors: P
authors: Sung
mesh_terms: D020816:Amino Acid Motifs; D019313:BRCA1 Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D016601:RNA-Binding Proteins; D051135:Rad51 Recombinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C110410:RAD51AP1 protein, human; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; C495153:RAD51 protein, human; D051135:Rad51 Recombinase; C074556:DMC1 protein, human