title: Effect of exposure of human monocyte-derived macrophages to high, versus normal, glucose on subsequent lipid accumulation from glycated and acetylated low-density lipoproteins.
abstract: During atherosclerosis monocyte-derived macrophages accumulate cholesteryl esters from low-density lipoproteins (LDLs) via lectin-like oxidised LDL receptor-1 (LOX-1) and class AI and AII (SR-AI, SR-AII) and class B (SR-BI, CD36) scavenger receptors. Here we examined the hypothesis that hyperglycaemia may modulate receptor expression and hence lipid accumulation in macrophages. Human monocytes were matured into macrophages in 30 versus 5 mM glucose and receptor expression and lipid accumulation quantified. High glucose elevated LOX1 mRNA, but decreased SR-AI, SR-BI, LDLR, and CD36 mRNA. SR-BI and CD36 protein levels were decreased. Normo- and hyperglycaemic cells accumulated cholesteryl esters from modified LDL to a greater extent than control LDL, but total and individual cholesteryl ester accumulation was not affected by glucose levels. It is concluded that, whilst macrophage scavenger receptor mRNA and protein levels can be modulated by high glucose, these are not key factors in lipid accumulation by human macrophages under the conditions examined.
authors: Free Radical Group, The Heart Research Institute, 7 Eliza Street, Newtown, Sydney, NSW 2042, Australia.
authors: Fatemeh
authors: F
authors: Moheimani
authors: Joanne T M
authors: JT
authors: Tan
authors: Bronwyn E
authors: BE
authors: Brown
authors: Alison K
authors: AK
authors: Heather
authors: David M
authors: DM
authors: van Reyk
authors: Michael J
authors: MJ
authors: Davies
mesh_terms: D050197:Atherosclerosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002788:Cholesterol Esters / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005947:Glucose / Q000008:administration & dosage* / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D051117:Scavenger Receptors, Class A / Q000235:genetics / Q000378:metabolism; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000378:metabolism; D000093844:Glycated Proteins
chemical_list: D018955:CD36 Antigens; D002788:Cholesterol Esters; D017931:DNA Primers; D005947:Glucose; D017127:Glycation End Products, Advanced; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D011973:Receptors, LDL; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; D051127:Scavenger Receptors, Class E; C495139:MSR1 protein, human; C495137:OLR1 protein, human; C495124:SCARB1 protein, human; C032629:acetyl-LDL; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins