title: Coordinated regulation of ATF2 by miR-26b in γ-irradiated lung cancer cells.
abstract: MicroRNA regulates cellular responses to ionizing radiation (IR) through translational control of target genes. We analyzed time-series changes in microRNA expression following γ-irradiation in H1299 lung cancer cells using microarray analysis. Significantly changed IR-responsive microRNAs were selected based on analysis of variance analysis, and predicted target mRNAs were enriched in mitogen-activated protein kinase (MAPK) signaling. Concurrent analysis of time-series mRNA and microRNA profiles uncovered that expression of miR-26b was down regulated, and its target activating transcription factor 2 (ATF2) mRNA was up regulated in γ-irradiated H1299 cells. IR in miR-26b overexpressed H1299 cells could not induce expression of ATF2. When c-Jun N-terminal kinase activity was inhibited using SP600125, expression of miR-26b was induced following γ-irradiation in H1299 cells. From these results, we concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway.
authors: Department of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea.
authors: Himanshu
authors: H
authors: Arora
authors: Rehana
authors: R
authors: Qureshi
authors: Ae-Kyung
authors: AK
authors: Park
authors: Woong-Yang
authors: WY
authors: Park
mesh_terms: D051698:Activating Transcription Factor 2 / Q000235:genetics*; D000873:Anthracenes / Q000494:pharmacology; D045744:Cell Line, Tumor; D005720:Gamma Rays; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects
chemical_list: D051698:Activating Transcription Factor 2; D000873:Anthracenes; D035683:MicroRNAs; D020928:Mitogen-Activated Protein Kinases; D016755:Proto-Oncogene Proteins c-jun; C528163:MIRN26 microRNA, human; C432165:pyrazolanthrone