title: Chemical modification of proteins to improve the accuracy of their relative molecular mass determination by electrophoresis.
abstract: We studied the electrophoretic behavior of basic proteins (cytochrome c and histone III) and developed a carbamylation method that normalizes their electrophoretic size separation and improves the accuracy of their relative molecular mass determined electrophoretically. In capillary zone electrophoresis with cationic hitchhiking, native cytochrome c does not sufficiently bind cationic surfactants due to electrostatic repulsion between the basic protein and cationic surfactant. Carbamylation suppresses the strong positive charge of the basic proteins and results in more accurate relative molecular masses.
authors: Alcor BioSeparations LLC, Palo Alto, CA, USA. ladia@dolnik.net
authors: Vladislav
authors: V
authors: Dolnik
authors: William A
authors: WA
authors: Gurske
mesh_terms: D000818:Animals; D001669:Biochemical Phenomena; D002412:Cations / Q000737:chemistry; D002417:Cattle; D045304:Cytochromes c / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D010720:Phosphines / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D002412:Cations; D045304:Cytochromes c; D010720:Phosphines; D011506:Proteins; C080938:tris(2-carboxyethyl)phosphine