title: New multilocus sequence typing of MRSA in São Paulo, Brazil.
abstract: An increased incidence of nosocomial and community-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has been observed worldwide. The molecular characterization of MRSA has played an important role in demonstrating the existence of internationally disseminated clones. The use of molecular biology methods in the surveillance programs has enabled the tracking of MRSA spread within and among hospitals. These data are useful to alert nosocomial infection control programs about the potential introduction of these epidemic clones in their areas. Four MRSA blood culture isolates from patients hospitalized at two hospitals in the city of São Paulo, Brazil, were analyzed; one of them was community acquired. The isolates were characterized as SCCmec, mecA and PVL by PCR, pulsed-field gel electrophoresis (PFGE) profile and molecular sequence typing (MLST) genotyping. The isolates presented type IV SCCmec, and none proved to be positive for PVL. The isolates showed a PFGE profile similar to the pediatric clone. MLST genotyping demonstrated that the isolates belonged to clonal complex 5 (CC5), showing a new yqiL allele gene, resulting in a new sequence typing (ST) (1176). Our results showed that strains of MRSA carrying a new ST are emerging in community and nosocomial infections, including bacteremia, in São Paulo, Brazil.
authors: Laboratório Especial de Microbiologia Clínica, Departamento de Doenças Infecciosas e Parasitárias, Universidade Federal de São Paulo, São Paulo, SP, Brasil.
authors: M S
authors: MS
authors: Carmo
authors: F
authors: F
authors: Inoue
authors: S S
authors: SS
authors: Andrade
authors: L
authors: L
authors: Paschoal
authors: F M
authors: FM
authors: Silva
authors: V G S
authors: VG
authors: Oliveira
authors: A C C
authors: AC
authors: Pignatari
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001938:Brazil; D017714:Community-Acquired Infections / Q000382:microbiology; D003428:Cross Infection / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification; D058885:Multilocus Sequence Typing / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins