title: Characterization of the Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative Standard using SDS-PAGE shotgun proteomics.
abstract: Although there are now multiple methods for the analysis of membrane proteomes, there is relatively little systematic characterization of proteomic workflows for membrane proteins. The Asia Oceania Human Proteome Organisation (AOHUPO) has therefore embarked on a Membrane Proteomics Initiative (MPI) using a large range of workflows. Here, we describe the characterization of the MPI mouse liver microsomal membrane Standard using SDS-PAGE prior to in-gel tryptic digestion and LC-ESI-MS/MS. The Na(2) CO(3)  wash followed by SDS-PAGE prior to in-gel tryptic digestion and LC-MS/MS strategy was effective for the detection of membrane proteins with 47.1% of the identified proteins being transmembrane proteins. Gene Ontology term enrichment analysis showed that biological processes involving transport, lipid metabolism, cell communication, cell adhesion, and cellular component organization were significantly enriched. Comparison of the present data with the previously published reports on mouse liver proteomes confirmed that the MPI Standard provides an excellent resource for the analysis of membrane proteins in the AOHUPO MPI.
authors: School of Biological Sciences and Centre for Biodiscovery, Victoria University of Wellington, Wellington, New Zealand.
authors: Lifeng
authors: L
authors: Peng
authors: Eugene A
authors: EA
authors: Kapp
authors: Danyl
authors: D
authors: McLauchlan
authors: T William
authors: TW
authors: Jordan
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry*; D002853:Chromatography, Liquid; D016000:Cluster Analysis; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D017261:Glycosylphosphatidylinositols / Q000032:analysis / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D008862:Microsomes, Liver / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods*; D040901:Proteomics / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry
chemical_list: D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D010446:Peptide Fragments