title: Mutation rate of bacteriophage ΦX174 modified through changes in GATC sequence context.
abstract: Bacteriophage ΦX174 has a relatively high mutation rate of 10⁻⁶ substitutions per nucleotide per strand copying. A thirty-fold reduction in the mutation rate was achieved by introducing seven GATC sequences in its genome. This motif allows for methyl-directed mismatch repair and is strongly avoided in nature by ΦX174 and other phages.
authors: Instituto Cavanilles de Biodiversidad y Biología Evolutiva and Departamento de Genética, Universitat de València, Spain.
authors: José M
authors: JM
authors: Cuevas
authors: Marianoel
authors: M
authors: Pereira-Gómez
authors: Rafael
authors: R
authors: Sanjuán
mesh_terms: D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D019175:DNA Methylation / Q000235:genetics; D053843:DNA Mismatch Repair; D004279:DNA, Viral / Q000235:genetics*; D019143:Evolution, Molecular; D016679:Genome, Viral; D009154:Mutation
chemical_list: D004279:DNA, Viral