title: A standardized immunofluorescence test method with human neutrophil antigen-expressing cell lines to enhance antibody detection.
abstract: There is an international need for a large-scale human neutrophils antigen (HNA) antibody screening platform to minimize the risk of antibody-mediated transfusion-related acute lung injury. However, sourcing a substantial, reliable source of HNA, as well as the scarcity of well-characterized HNA antisera for validating new screening platforms, remain as major obstacles. This short communication presents an improved protocol for the effective use of HNA-expressing KY cells as a screening platform using eight well-characterized HNA antisera of a single defined specificity.
authors: Australian Red Cross Blood Service, Brisbane, Qld, Australia.
authors: G H
authors: GH
authors: Lopez
authors: M M
authors: MM
authors: Dean
authors: K
authors: K
authors: Yasui
authors: R M
authors: RM
authors: Schuller
authors: F
authors: F
authors: Hirayama
authors: Y L
authors: YL
authors: Fung
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D002460:Cell Line; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D007519:Isoantigens / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D014162:Transfection
chemical_list: D000906:Antibodies; D007518:Isoantibodies; D007519:Isoantigens