title: Optimization of adenovirus vectors for transduction in embryonic stem cells and induced pluripotent stem cells.
abstract: Because embryonic stem (ES) cells and induced pluripotent stem (iPS) cells can differentiate into various types of cells in vitro, they are considered as a valuable model to understand the processes involved in the differentiation into functional cells as well as an unlimited source of cells for therapeutic applications. Efficient gene transduction method is one of the powerful tools for the basic researches and for differentiating ES and iPS cells into lineage-committed cells. Recently, we have developed an adenovirus (Ad) vector for efficient transduction into ES and iPS cells. We showed that Ad vectors containing the cytomegalovirus enhancer/β-actin promoter with β-actin intron (CA) promoter or the elongation factor (EF)-1α promoter were the appropriate for the transduction into ES and iPS cells. We also found that enforced expression of a PPARγ gene or a Runx2 gene into mouse ES and iPS cells by an optimized Ad vector markedly augmented the differentiation of adipocytes or osteoblasts, respectively. Thus, a gene transfer technique using an Ad vector could be an advantage for the regulation of stem cell differentiation and could be applied to regenerative medicine based on ES and iPS cells.
authors: Laboratory of Stem Cell Regulation, National Institute of Biomedical Innovation, Osaka, Japan. tashiro@nibio.go.jp
authors: Katsuhisa
authors: K
authors: Tashiro
mesh_terms: D000256:Adenoviridae / Q000737:chemistry; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D053595:Embryonic Stem Cells / Q000166:cytology*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D028322:Inhibin-beta Subunits; D051379:Mice; D020648:Peptide Elongation Factor 1; D011401:Promoter Regions, Genetic; D044968:Regenerative Medicine / Q000379:methods
chemical_list: D020648:Peptide Elongation Factor 1; D028322:Inhibin-beta Subunits