title: Comparison of the in vitro storage properties of Amicus apheresis platelets collected using single- and double-needle procedures from the same donors.
abstract: BACKGROUND
  Amicus apheresis platelets (PLTs) can be collected using either a single- (SN) or a double-needle (DN) procedure. To investigate whether the method of PLT collection using the same instrument influences PLT quality, the in vitro storage properties of Amicus PLTs were evaluated in the same donors collected by SN and DN procedures.
  
  
  METHODS
  Single apheresis PLT collections with concurrent plasma were performed on donors using the Amicus with a target yield of 4 × 10(11). A PLT unit was collected from a donor assigned to either a SN or a DN procedure; a subsequent donation from the same individual was collected by the other procedure (n = 10). Units were stored at 20 to 24°C with continuous agitation, assayed for 19 PLT storage variables, and analyzed by paired t test, with differences between values obtained with SN and DN collections considered significant with p values of less than 0.001.
  
  
  RESULTS
  PLT units collected by SN procedure had contents and concentrations similar to those collected by DN procedures (4.1 × 10(11) ± 0.3 × 10(11) vs. 4.0 × 10(11) ± 0.3 × 10(11) and 1396 × 10(9) ± 131 × 10(9) vs. 1367 × 10(9) ± 110 × 10(9) PLTs/L). On Day 7, SN and DN PLTs had comparable pH values (7.07 ± 0.09 vs. 6.99 ± 0.17), morphology (52.4 ± 18.7% vs. 56.0 ± 13.3% discoid), aggregation (87.1 ± 11.5% vs. 91.3 ± 5.4%), and activation (45. ± 11.9% vs. 48.2 ± 8.7% CD62P), as well as all other variables (p > 0.05; Day 7 CO(2) , p = 0.0304).
  
  
  CONCLUSIONS
  The in vitro storage properties of apheresis PLTs collected from the same donors using a SN and DN procedure with the Amicus instrument were maintained through 7 days of storage and yielded comparable results.
authors: Blood Components Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA. wagners@usa.redcross.org
authors: Stephen J
authors: SJ
authors: Wagner
authors: Shalini
authors: S
authors: Seetharaman
authors: James
authors: J
authors: Kurtz
mesh_terms: D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology*; D001793:Blood Preservation / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D009339:Needles / Q000592:standards*; D010983:Plateletpheresis / Q000295:instrumentation* / Q000379:methods* / Q000592:standards