title: Gold nanoparticles for high-throughput genotyping of long-range haplotypes.
abstract: Completion of the Human Genome Project and the HapMap Project has led to increasing demands for mapping complex traits in humans to understand the aetiology of diseases. Identifying variations in the DNA sequence, which affect how we develop disease and respond to pathogens and drugs, is important for this purpose, but it is difficult to identify these variations in large sample sets. Here we show that through a combination of capillary sequencing and polymerase chain reaction assisted by gold nanoparticles, it is possible to identify several DNA variations that are associated with age-related macular degeneration and psoriasis on significant regions of human genomic DNA. Our method is accurate and promising for large-scale and high-throughput genetic analysis of susceptibility towards disease and drug resistance.
authors: Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200030, China.
authors: Peng
authors: P
authors: Chen
authors: Dun
authors: D
authors: Pan
authors: Chunhai
authors: C
authors: Fan
authors: Jianhua
authors: J
authors: Chen
authors: Ke
authors: K
authors: Huang
authors: Dongfang
authors: D
authors: Wang
authors: Honglu
authors: H
authors: Zhang
authors: You
authors: Y
authors: Li
authors: Guoyin
authors: G
authors: Feng
authors: Peiji
authors: P
authors: Liang
authors: Lin
authors: L
authors: He
authors: Yongyong
authors: Y
authors: Shi
mesh_terms: D004351:Drug Resistance / Q000235:genetics; D015894:Genome, Human; D006046:Gold / Q000737:chemistry*; D006239:Haplotypes; D006801:Humans; D008268:Macular Degeneration / Q000235:genetics*; D053768:Metal Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011565:Psoriasis / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D006046:Gold