title: Effects of peanut-skin procyanidin A1 on degranulation of RBL-2H3 cells.
abstract: Peanut skin contains large amounts of polyphenols having antiallergic effects. We found that a peanut-skin extract (PSE) inhibits the degranulation induced by antigen stimulation of rat basophilic leukemia (RBL-2H3) cells. A low-molecular-weight fraction from PSE, PSEL, also had inhibitory activity against allergic degranulation. A main polyphenol in PSEL was purified by gel chromatography and fractionated by YMC-gel ODS-AQ 120S50 column. Electrospray ionization mass spectrometry (ESI-MS) analysis of the purified polyphenol gave m/z 599 [M+Na]⁺. Based on the results of ¹H-NMR, ¹³C-NMR spectra, and optical rotation analysis, the polyphenol was identified as procyanidin A1. It inhibited the degranulation caused by antigen stimulation at the IC₅₀ of 20.3 µM. Phorbol-12-myristate-13-acetate (PMA) and 2,5,-di(tert-butyl)-1,4-hydroquinone (DTBHQ)-induced processes of degranulation were also inhibited by procyanidin A1. These results indicate that peanut-skin procyanidin A1 inhibits degranulation downstream of protein kinase C activation or Ca²⁺ influx from an internal store in RBL-2H3 cells.
authors: Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture.
authors: Keisuke
authors: K
authors: Tomochika
authors: Akiko
authors: A
authors: Shimizu-Ibuka
authors: Tomoko
authors: T
authors: Tamura
authors: Kiyoshi
authors: K
authors: Mura
authors: Naoki
authors: N
authors: Abe
authors: Jun-Ichi
authors: J
authors: Onose
authors: Soichi
authors: S
authors: Arai
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010367:Arachis / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002392:Catechin / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D045744:Cell Line, Tumor; D006873:Hydroquinones / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006967:Hypersensitivity / Q000188:drug therapy / Q000276:immunology / Q000517:prevention & control*; D015471:Leukemia, Basophilic, Acute / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D059808:Polyphenols / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D044945:Proanthocyanidins / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D012639:Seeds / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D006873:Hydroquinones; D010936:Plant Extracts; D059808:Polyphenols; D044945:Proanthocyanidins; C520684:procyanidin A1; C019359:2,5-di-tert-butylhydroquinone; D002392:Catechin; D001619:beta-N-Acetylhexosaminidases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium