title: Genotypes, haplotypes and diplotypes of IGF-II SNPs and their association with growth traits in largemouth bass (Micropterus salmoides).
abstract: Insulin-like growth factor II (IGF-II) is involved in the regulation of somatic growth and metabolism in many fishes. IGF-II is an important candidate gene for growth traits in fishes and its polymorphisms were associated with the growth traits. The aim of this study is to screen single nucleotide polymorphisms (SNPs) of the largemouth bass (Micropterus salmoides) IGF-II gene and to analyze potential association between IGF-II gene polymorphisms and growth traits in largemouth bass. Four SNPs (C127T, T1012G, C1836T and C1861T) were detected and verified by DNA sequencing in the largemouth bass IGF-II gene. These SNPs were found to organize into seven haplotypes, which formed 13 diplotypes (haplotype pairs). Association analysis showed that four individual SNPs were not significantly associated with growth traits. Significant associations were, however, noted between diplotypes and growth traits (P < 0.05). The fish with H1H3 (CTCC/CGCC) and H1H5 (CTCC/TTTT) had greater body weight than those with H1H1 (CTCC/CTCC), H1H2 (CTCC/TGTT) and H4H4 (TGCT/TGCT/) did. Our data suggest a significant association between genetic variations in the largemouth bass IGF-II gene and growth traits. IGF-II SNPs could be used as potential genetic markers in future breeding programs of largemouth bass.
authors: Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture of the People's Republic of China, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, Guangdong, People's Republic of China. lxh152706@126.com
authors: Xiaohui
authors: X
authors: Li
authors: Junjie
authors: J
authors: Bai
authors: Yinchang
authors: Y
authors: Hu
authors: Xing
authors: X
authors: Ye
authors: Shengjie
authors: S
authors: Li
authors: Lingyun
authors: L
authors: Yu
mesh_terms: D000818:Animals; D001492:Bass / Q000235:genetics* / Q000254:growth & development*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D016678:Genome / Q000235:genetics; D006239:Haplotypes / Q000235:genetics*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable
chemical_list: D007335:Insulin-Like Growth Factor II