title: Light-based feedback for controlling intracellular signaling dynamics.
abstract: The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
authors: Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, California, USA.
authors: Jared E
authors: JE
authors: Toettcher
authors: Delquin
authors: D
authors: Gong
authors: Wendell A
authors: WA
authors: Lim
authors: Orion D
authors: OD
authors: Weiner
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D019295:Computational Biology; D025461:Feedback, Physiological / Q000528:radiation effects*; D006801:Humans; D008027:Light; D008954:Models, Biological; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011485:Protein Binding; D015398:Signal Transduction / Q000528:radiation effects*
chemical_list: D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors