title: Enhancement of the skin-protective activities of Centella asiatica L. Urban by a nano-encapsulation process.
abstract: Aqueous extracts of Centella asiatica L. Urban were encapsulated by an edible biopolymer, gelatin, which has no effect on their cosmetic activities. The nanoparticles were w/o-type spherical liposomes that had an average diameter of 115.0nm. The encapsulation efficiency was estimated to be approximately 67%, which was relatively high for these aqueous extracts. The nanoparticles showed lower cytotoxicity (10%) in human skin fibroblast cells than the unencapsulated crude extract (15%) at 1.0mg/ml, this was possibly because a smaller amount of the extract was present in the nanoparticles. The nanoparticles efficiently reduced the expression of matrix metalloproteinase (MMP)-1 in UV-irradiated cells from 136.1% to 77.6% (UV-irradiated control) and inhibited hyaluronidase expression (>60%) at a concentration of 0.5mg/ml, which was higher than the levels produced by the unencapsulated crude extracts. The nanoparticles had a very high flux through mouse skin and also remained at relatively large concentrations in the derma when compared to the unencapsulated crude extracts. These results clearly indicate that the skin-protective activities of C. asiatica were significantly improved through the nano-encapsulation process. These findings also imply that a crude extract can be used and have the same efficacy as purified compounds, which should reduce the purification process and production costs.
authors: Department of Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, South Korea.
authors: Min Chul
authors: MC
authors: Kwon
authors: Woon Yong
authors: WY
authors: Choi
authors: Yong Chang
authors: YC
authors: Seo
authors: Ji Seon
authors: JS
authors: Kim
authors: Chang Soon
authors: CS
authors: Yoon
authors: Hye Won
authors: HW
authors: Lim
authors: Hack Soo
authors: HS
authors: Kim
authors: Ju hee
authors: Jh
authors: Ahn
authors: Hyeon Yong
authors: HY
authors: Lee
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001704:Biopolymers / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D028041:Centella / Q000737:chemistry*; D002645:Chickens; D003358:Cosmetics; D005260:Female; D005347:Fibroblasts; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D053769:Nanocapsules / Q000737:chemistry*; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism
chemical_list: D001704:Biopolymers; D003358:Cosmetics; D053769:Nanocapsules; D010936:Plant Extracts; D020011:Protective Agents; D005780:Gelatin; D006821:Hyaluronoglucosaminidase; D020781:Matrix Metalloproteinase 1