title: Multivalent interaction and selectivities in selectin binding of functionalized gold colloids decorated with carbohydrate mimetics.
abstract: Colloidal gold particles with functionalized organic shells were applied as novel selectin binders. The ligand shell was terminated with different monocyclic carbohydrate mimetics as simplified analogs of the sLe(x) unit found in biological selectin ligands. The multivalent presentation of the sulfated selectin binding epitopes on the gold particles led to extremely high binding affinities towards L- and P-selectin and IC(50) values in the subnanomolar range. Depending on the ring size of the sulfated carbohydrate mimetic, its substitution pattern and its configuration, different selectivities for either L-selectin or P-selectin were obtained. These selectivities were not found for gold particles with simple acyclic sulfated alcohols, diols and triols in the ligand shell. In addition, the influence of the particle size and the thickness of the hydrophobic organic shell were systematically investigated.
authors: Freie Universität Berlin, Institut für Chemie und Biochemie, Takustr. 3, 14195, Berlin, Germany.
authors: Meike
authors: M
authors: Roskamp
authors: Sven
authors: S
authors: Enders
authors: Fabian
authors: F
authors: Pfrengle
authors: Shahla
authors: S
authors: Yekta
authors: Vjekoslav
authors: V
authors: Dekaris
authors: Jens
authors: J
authors: Dernedde
authors: Hans-Ulrich
authors: HU
authors: Reissig
authors: Sabine
authors: S
authors: Schlecht
mesh_terms: D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry*; D002470:Cell Survival; D003102:Colloids / Q000138:chemical synthesis / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D019169:Jurkat Cells; D008968:Molecular Conformation; D018716:Molecular Mimicry; D019087:Selectins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D003102:Colloids; D019087:Selectins; D013438:Sulfhydryl Compounds; D006046:Gold