title: Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology.
abstract: Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects of ionizing radiation are available. Here we describe the isolation of mouse primary cardiac endothelial cells, a possible target for cardiotoxic effects of radiation. Cells isolated from hearts of juvenile mice were cultured and irradiated in vitro. In addition, cells isolated from hearts of locally irradiated adult animals (up to 6 days after irradiation) were tested. A dose-dependent formation of histone γH2A.X foci was observed after in vitro irradiation of cultured cells. However, such cells were resistant to radiation-induced apoptosis. Increased levels of actin stress fibres were observed in the cytoplasm of cardiac endothelial cells irradiated in vitro or isolated from irradiated animals. A high dose of 16 Gy did not increase permeability to Dextran in monolayers formed by endothelial cells. Up-regulated expression of Vcam1, Sele and Hsp70i genes was detected after irradiation in vitro and in cells isolated few days after irradiation in vivo. The increased level of actin stress fibres and enhanced expression of stress-response genes in irradiated endothelial cells are potentially involved in cardiotoxic effects of ionizing radiation.
authors: Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland.
authors: Karol
authors: K
authors: Jelonek
authors: Anna
authors: A
authors: Walaszczyk
authors: Dorota
authors: D
authors: Gabryś
authors: Monika
authors: M
authors: Pietrowska
authors: Chryso
authors: C
authors: Kanthou
authors: Piotr
authors: P
authors: Widłak
mesh_terms: D000199:Actins / Q000528:radiation effects; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002478:Cells, Cultured; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D019040:E-Selectin / Q000235:genetics; D042783:Endothelial Cells / Q000528:radiation effects*; D005786:Gene Expression Regulation / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006321:Heart / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009206:Myocardium / Q000166:cytology*; D010539:Permeability; D011839:Radiation, Ionizing; D011853:Radiobiology / Q000379:methods*; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D000199:Actins; D019040:E-Selectin; D018840:HSP70 Heat-Shock Proteins; D019010:Vascular Cell Adhesion Molecule-1