title: Early second-trimester serum miRNA profiling predicts gestational diabetes mellitus.
abstract: BACKGROUND
  Gestational diabetes mellitus (GDM) is one type of diabetes that presents during pregnancy and significantly increases the risk of a number of adverse consequences for the fetus and mother. The microRNAs (miRNA) have recently been demonstrated to abundantly and stably exist in serum and to be potentially disease-specific. However, no reported study investigates the associations between serum miRNA and GDM.
  
  
  RESULTS
  We systematically used the TaqMan Low Density Array followed by individual quantitative reverse transcription polymerase chain reaction assays to screen miRNAs in serum collected at 16-19 gestational weeks. The expression levels of three miRNAs (miR-132, miR-29a and miR-222) were significantly decreased in GDM women with respect to the controls in similar gestational weeks in our discovery evaluation and internal validation, and two miRNAs (miR-29a and miR-222) were also consistently validated in two-centric external validation sample sets. In addition, the knockdown of miR-29a could increase Insulin-induced gene 1 (Insig1) expression level and subsequently the level of Phosphoenolpyruvate Carboxy Kinase2 (PCK2) in HepG2 cell lines.
  
  
  CONCLUSIONS
  Serum miRNAs are differentially expressed between GDM women and controls and could be candidate biomarkers for predicting GDM. The utility of miR-29a, miR-222 and miR-132 as serum-based non-invasive biomarkers warrants further evaluation and optimization.
authors: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
authors: Chun
authors: C
authors: Zhao
authors: Jing
authors: J
authors: Dong
authors: Tao
authors: T
authors: Jiang
authors: Zhonghua
authors: Z
authors: Shi
authors: Bin
authors: B
authors: Yu
authors: Yunlong
authors: Y
authors: Zhu
authors: Daozhen
authors: D
authors: Chen
authors: Junrong
authors: J
authors: Xu
authors: Ran
authors: R
authors: Huo
authors: Juncheng
authors: J
authors: Dai
authors: Yankai
authors: Y
authors: Xia
authors: Shiyang
authors: S
authors: Pan
authors: Zhibin
authors: Z
authors: Hu
authors: Jiahao
authors: J
authors: Sha
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016640:Diabetes, Gestational / Q000175:diagnosis*; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D011237:Predictive Value of Tests; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C547077:MIRN132 microRNA, human; C525710:MIR222, human; C532045:MIRN29a microRNA, human