title: LC-MS/MS profiling for detection of endogenous steroids and prostaglandins in tissue samples.
abstract: Roles of steroid hormones, and compounds that can influence their levels in cells, are of increasing interest in e.g. cancer research, partly because resistance to hormone therapies often complicates treatment. To elucidate the processes involved, the hormones and related compounds need to be accurately measured. Reversed-phase liquid chromatography with dynamic multiple reaction monitoring mass spectrometric detection in electrospray mode is capable of providing such measurements. Therefore, LC-MS/MS was developed for sensitive, selective analysis of 11 steroid hormones, cholesterol and two prostaglandins. The effects of the tissue matrix, and solid-phase extraction (SPE) sample clean-up, on the LC-MS/MS signals of the hormones were also investigated. The results show that the developed LC-MS/MS method, following SPE clean-up to reduce matrix interference, can detect selected steroids in extracts of mouse tissues. The method provides linear measurements of the steroids at concentrations up to few ng/μL, and limits of detection in the range 0.03-0.2 pg/μL (for some compounds lower than those of previously reported methods).
authors: Department of Chemistry, Computational Life Science Cluster, Umeå University, Umeå, Sweden.
authors: Izabella
authors: I
authors: Surowiec
authors: Mariusz
authors: M
authors: Koc
authors: Henrik
authors: H
authors: Antti
authors: Pernilla
authors: P
authors: Wikström
authors: Thomas
authors: T
authors: Moritz
mesh_terms: D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D008297:Male; D051379:Mice; D010053:Ovary / Q000737:chemistry; D011453:Prostaglandins / Q000032:analysis*; D011467:Prostate / Q000737:chemistry; D013256:Steroids / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013737:Testis / Q000737:chemistry
chemical_list: D012739:Gonadal Steroid Hormones; D011453:Prostaglandins; D013256:Steroids