title: Isolation, characterization and docking studies of 2,3-dihydroxybiphenyl 1,2-dioxygenase from an activated sludge metagenome.
abstract: A 2,3-dihydroxybiphenyl-1,2-dioxygenase gene (designated as bphC_meta) was identified in activated sludge metagenome by PCR. This gene shared 99% sequence identity with BphC from Burkholderia xenovorans LB400. The enzyme was purified from recombinant Escherichia coli with a subunit molecular mass of 32 ± 1 kDa. It was optimally active at pH 9.0 and 40°C, using 2,3-dihydroxybiphenyl as a substrate. Activity toward substituted catechols was: 2,3-dihydroxybiphenyl > 3-methylcatechol > catechol > 4-chlorocatechol (4-methylcatechol). The prediction made by molecular docking was consistent with the kinetic experimental data, and further explained the substrate preference of BphC_meta. The present study could pave the way for the improved understanding and application of BphCs derived from metagenomes.
authors: State Key Laboratory of Fine Chemicals and Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Dalian, 116024, China.
authors: Min
authors: M
authors: Gou
authors: Yuanyuan
authors: Y
authors: Qu
authors: Bingwen
authors: B
authors: Xu
authors: Jiti
authors: J
authors: Zhou
authors: Xinliang
authors: X
authors: Li
authors: Hao
authors: H
authors: Zhou
mesh_terms: D001713:Biphenyl Compounds / Q000378:metabolism*; D019117:Burkholderia / Q000201:enzymology / Q000235:genetics; D002396:Catechols / Q000378:metabolism*; D049308:Dioxygenases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D054892:Metagenome; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012722:Sewage / Q000382:microbiology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001713:Biphenyl Compounds; D002396:Catechols; D011994:Recombinant Proteins; D012722:Sewage; C454813:2,3-dihydroxybiphenyl; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase