title: A first generation microsatellite- and SNP-based linkage map of Jatropha.
abstract: Jatropha curcas is a potential plant species for biodiesel production. However, its seed yield is too low for profitable production of biodiesel. To improve the productivity, genetic improvement through breeding is essential. A linkage map is an important component in molecular breeding. We established a first-generation linkage map using a mapping panel containing two backcross populations with 93 progeny. We mapped 506 markers (216 microsatellites and 290 SNPs from ESTs) onto 11 linkage groups. The total length of the map was 1440.9 cM with an average marker space of 2.8 cM. Blasting of 222 Jatropha ESTs containing polymorphic SSR or SNP markers against EST-databases revealed that 91.0%, 86.5% and 79.2% of Jatropha ESTs were homologous to counterparts in castor bean, poplar and Arabidopsis respectively. Mapping 192 orthologous markers to the assembled whole genome sequence of Arabidopsis thaliana identified 38 syntenic blocks and revealed that small linkage blocks were well conserved, but often shuffled. The first generation linkage map and the data of comparative mapping could lay a solid foundation for QTL mapping of agronomic traits, marker-assisted breeding and cloning genes responsible for phenotypic variation.
authors: Molecular Population Genetics Group, Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.
authors: Chun Ming
authors: CM
authors: Wang
authors: Peng
authors: P
authors: Liu
authors: Chengxin
authors: C
authors: Yi
authors: Keyu
authors: K
authors: Gu
authors: Fei
authors: F
authors: Sun
authors: Lei
authors: L
authors: Li
authors: Loong Chueng
authors: LC
authors: Lo
authors: Xiaokun
authors: X
authors: Liu
authors: Felicia
authors: F
authors: Feng
authors: Grace
authors: G
authors: Lin
authors: Suying
authors: S
authors: Cao
authors: Yan
authors: Y
authors: Hong
authors: Zhongchao
authors: Z
authors: Yin
authors: Gen Hua
authors: GH
authors: Yue
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D020224:Expressed Sequence Tags; D008040:Genetic Linkage / Q000235:genetics*; D018745:Genome, Plant / Q000235:genetics; D031286:Jatropha / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics