title: Crimp frequency is strongly correlated to myofibroblast density in the human anterior cruciate ligament and its autologous tendon grafts.
abstract: OBJECTIVE
  Collagen crimp is essential for maintaining viscoelastic properties of normal ligament and tendon tissue. The actin isoform α-smooth muscle actin (ASMA) has been identified in fibroblastic cells of these tissues. These highly differentiated cells, so-called myofibroblasts may transmit tensile forces to the extracellular matrix, thus it has been suggested that they are responsible for the wrinkling of the extracellular matrix and the formation of crimp. During anterior cruciate ligament (ACL) graft remodeling, crimp formation plays an integral role. Thus, it was our purpose to determine the relationship between myofibroblast density and crimp frequency in human tendon graft tissue and the ACL.
  
  
  METHODS
  Different tendon grafts and ACLs were harvested from young human multi-organ donors immediately after death. Myofibroblasts were immunostained with a monoclonal antibody, and histomorphometry was performed using a digital imaging system. Crimp length was measured, and data were correlated.
  
  
  RESULTS
  All tendons and ACLs showed a significant correlation of myofibroblast density and crimp frequency (R(2) 0.81-0.43). The strongest correlation was found for the patellar tendon, the poorest for the gracilis tendon. There is also evidence that the phenotype respectively the shape of myofibroblasts might be responsible for different stages of crimp formation.
  
  
  CONCLUSIONS
  With the present investigation, we found that myofibroblasts might be involved in crimp formation and should be viewed as an integral part of normal tendon and ligament tissue. Furthermore, the shape of myofibroblasts may further indicate the contractile potency of the extracellular matrix, thus presenting a dynamic and variable crimp rather than a static situation. This study is an experimental study. In terms of clinical relevance all the mentioned tendons can be used as auto- or allografts for ACL reconstruction, nevertheless their microscopic structure and cellular population have yet not been adequately investigated and compared.
authors: Klinik für Arthroskopische Chirurgie und Sporttraumatologie, Krankenhaus St. Josef, Bergstr. 6 - 12, 42105, Wuppertal, Germany. markus-weiss@email.de
authors: Markus
authors: M
authors: Weiss
authors: Frank N
authors: FN
authors: Unterhauser
authors: Andreas
authors: A
authors: Weiler
mesh_terms: D000199:Actins / Q000032:analysis; D000328:Adult; D016118:Anterior Cruciate Ligament / Q000737:chemistry / Q000166:cytology / Q000637:transplantation*; D002452:Cell Count; D005347:Fibroblasts / Q000637:transplantation*; D006801:Humans; D007150:Immunohistochemistry; D058628:Myofibroblasts / Q000166:cytology*; D017847:Patellar Ligament / Q000637:transplantation; D013710:Tendons / Q000166:cytology / Q000637:transplantation*; D014182:Transplantation, Autologous; D019737:Transplants
chemical_list: D000199:Actins