title: Assessment of antibody specificity using suspension bead arrays.
abstract: With the increasing collection of affinity reagents, their validation in terms of functionality and binding specificity becomes a challenge. To match this growing need, miniaturized and parallelized platforms have become available to corroborate the applicability for a broad range of binder scaffolds. Among the -commonly used systems, planar microarrays have been a platform of choice for a long time but alternative systems are emerging, of which one is based on color-coded beads for the creation of arrays in suspension. The latter systems offer to perform a two-dimensional multiplexing by now analyzing up to 384 samples against up to 500 analytes in a single experiment. While the analyte parameter is flexible in terms of its composition, an extended screening can be facilitated without the need to set up a microarray production facility.
authors: SciLifeLab Stockholm, KTH - Royal Institute of Technology, Tomtebodav, Sweden.
authors: Jochen M
authors: JM
authors: Schwenk
authors: Peter
authors: P
authors: Nilsson
mesh_terms: D000906:Antibodies / Q000378:metabolism; D000918:Antibody Specificity; D000941:Antigens / Q000378:metabolism*; D008863:Microspheres; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens