title: Induction of secretory leukocyte protease inhibitor (SLPI) in estradiol valerate (EV) induced polycystic ovary.
abstract: The excessive administration of estradiol valerate induces polycystic ovary syndrome by formation of follicular cysts. Secretory leukocyte protease inhibitor (SLPI) promotes wound healing by decreasing the excessive inflammatory response, stimulating keratinocyte proliferation and increasing collagen deposition through the inhibition of protease activity. In this study, SLPI expression was high in the ovarian stroma, corpus luteum, unilaminar primary follicle, multilaminar primary follicle and granulose layer of the antral follicle in polycystic ovary (PCO) compared to the normal ovary. SLPI was expressed strongly in the theca around the cyst in PCO compared to the mature follicle in the normal ovary. The levels of SLPI mRNA and protein expression were higher in PCO than in the normal ovary, and the level of MMP-2 expression was lower in PCO. These results showed that the formation of a cyst was initiated from a multilaminar primary follicle and SLPI expression was increased depending on the morphological changes in the follicle and ovarian stroma. Therefore, an increase in SLPI may be related to the suppression of tissue disruption, and act as a protease inhibitor in PCO, suggesting that SLPI increases independently of the estrogen concentration in pathological tissues.
authors: Department of Oral Histology & Developmental Biology, School of Dentistry, Chosun University, Gwangju 501-759, Korea.
authors: Jin-Ju
authors: JJ
authors: Park
authors: Chun Sik
authors: CS
authors: Bae
authors: Baik-Dong
authors: BD
authors: Choi
authors: Soon-Jeong
authors: SJ
authors: Jeong
authors: Guanlin
authors: G
authors: Wang
authors: Do-Seon
authors: DS
authors: Lim
authors: Byung-Ock
authors: BO
authors: Kim
authors: Young-Sik
authors: YS
authors: Cho
authors: Sun-Ju
authors: SJ
authors: Kim
authors: Moon-Jin
authors: MJ
authors: Jeong
mesh_terms: D000818:Animals; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D053481:Secretory Leukocyte Peptidase Inhibitor / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D053481:Secretory Leukocyte Peptidase Inhibitor; C506816:Slpi protein, rat; D004958:Estradiol; D020782:Matrix Metalloproteinases