title: Application of preparative high-speed counter-current chromatography for the separation of two alkaloids from the roots of Tabernaemontana catharinensis (Apocynaceae).
abstract: The methanolic extract of Tabernaemontana catharinensis (Apocynaceae) roots, which contains alkaloids with several biological activities, was separated on a preparative scale using high-speed counter-current chromatography. The optimum solvent system was found to be a mixture of CHCl(3)-MeOH-H(2)O [5:10:6 (v/v/v)] and led to a successful separation of two monoterpenic indole alkaloids, voachalotine (1) and 12-methoxy-N(b)-methylvoachalotine (2) in approximately 4.0 hours. The alkaloids were all isolated at purities over 95%, and their structures were established on the basis of spectroscopic methods, including 1D and 2D NMR and EI/MS.
authors: Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil.
authors: Milena S
authors: MS
authors: Gonçalves
authors: Ivo J Curcino
authors: IJ
authors: Vieira
authors: Rodrigo R
authors: RR
authors: Oliveira
authors: Raimundo
authors: R
authors: Braz-Filho
mesh_terms: D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution / Q000379:methods*; D000078183:Oxindoles; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry*; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D013141:Spiro Compounds; D030014:Tabernaemontana / Q000737:chemistry*
chemical_list: C568717:12-methoxy-N(b)-voachalotine; D000078183:Oxindoles; D010936:Plant Extracts; D046948:Secologanin Tryptamine Alkaloids; D013141:Spiro Compounds; C568718:voachalotine