title: Role of macrophages in early protective immune responses induced by two vaccines against foot and mouth disease.
abstract: Foot and Mouth Disease (FMD) is an acute disease of cloven-hoofed species. We studied the protection and early immune response induced in the murine model by vaccines formulated with inactivated virus and two different adjuvants. The presence of IMS12802PR or ISA206VG adjuvants yielded protection against viral challenge at early times post vaccination and induced FMDV-specific, but non neutralizing, antibody titers. In vivo macrophage depletion in vaccinated mice severely decreased the protection levels after virus challenge, indicating a central role of this cell population in the response elicited by the vaccines. Accordingly, opsonophagocytosis of FITC-labelled virus was augmented in 802-FMDVi and 206-FMDVi vaccinated mice. These results demonstrate the ability of the studied adjuvants to enhance the protective responses of these inactivated vaccines without the increase in seroneutralizing antibodies and the main role of opsonization and phagocytosis in the early protective immune responses against FMD infection in the murine model.
authors: Instituto de Virología-CICVyA, INTA Castelar, Nicolás Repetto y los Reseros s/n°, 1686 Hurlingham, Buenos Aires, Argentina. vquattrocchi@cnia.inta.gov.ar
authors: V
authors: V
authors: Quattrocchi
authors: C
authors: C
authors: Langellotti
authors: J S
authors: JS
authors: Pappalardo
authors: V
authors: V
authors: Olivera
authors: S
authors: S
authors: Di Giacomo
authors: N
authors: N
authors: van Rooijen
authors: C
authors: C
authors: Mongini
authors: C
authors: C
authors: Waldner
authors: P I
authors: PI
authors: Zamorano
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control*; D047589:Leukocyte Reduction Procedures; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D009895:Opsonin Proteins; D015164:Vaccines, Inactivated; D014765:Viral Vaccines