title: Time-dependent intermolecular interaction during protein reactions.
abstract: A recently developed method to monitor reaction kinetics of intermolecular interaction is presented in this perspective. This method is based on time-dependent diffusion coefficient measurements using the pulsed laser induced transient grating technique. Using this method, time dependent biomolecular interactions, such as transient association and dissociation reactions in solution, have been successfully detected in real time. The principles and particular applications are described. In particular, unique features of this time-dependent diffusion coefficient method are emphasized by comparison with other techniques.
authors: Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan. mterazima@kuchem.kyoto-u.ac.jp
authors: Masahide
authors: M
authors: Terazima
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004058:Diffusion; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D008958:Models, Molecular; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry / Q000378:metabolism; D055668:Photochemical Processes; D025941:Protein Interaction Mapping / Q000379:methods*; D055503:Protein Multimerization; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012996:Solutions; D046939:Synechocystis / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011506:Proteins; D012996:Solutions; D004247:DNA; D010727:Phosphoric Diester Hydrolases; C510176:bluF protein, E coli