title: Expression of interleukin-15 and interleukin-15Rα in monocytes of HIV type 1-infected patients with different courses of disease progression.
abstract: Interleukin-15 (IL-15) enhances the effector mechanisms of anti-HIV immune responses and thus is considered a potential adjuvant of HIV-1 vaccine. However, there are a lack of data concerning the relationships between IL-15 expression and regulation in HIV-1-infected patients and the course of disease progression. We found that IL-15, but not IL-15Rα, is expressed at significantly higher levels in the CD14(+) monocytes [stimulated or not with interferon (IFN)-γ] of long-term nonprogressors (LTNP) than in those of HIV-1 progressors or healthy controls. There was no between-group difference in the amounts of soluble IL-15 released from the cells. We also found that like the healthy controls, the LTNP expressed the IL-15 and IL-15Rα genes in a more coordinated manner than the progressors. Our findings show that there are significant differences in IL-15 expression between patients with different courses of HIV infection, and that the coordinated expression of the IL-15 and IL-15Rα genes is dysregulated in patients with progressive disease. They also provide important information concerning the mechanisms of infection and the potential use of IL-15 as a therapeutic agent.
authors: Department of Clinical Sciences, Section of Infectious Diseases and Tropical Medicine, University of Milan, Milan, Italy. maciej.tarkowski@unimi.it
authors: Maciej
authors: M
authors: Tarkowski
authors: Laurenzia
authors: L
authors: Ferraris
authors: Sara
authors: S
authors: Martone
authors: Francesco
authors: F
authors: Strambio de Castillia
authors: Donatella
authors: D
authors: Misciagna
authors: Renata I
authors: RI
authors: Mazzucchelli
authors: Emanuela
authors: E
authors: Lattuada
authors: Giuseppe
authors: G
authors: Paraninfo
authors: Massimo
authors: M
authors: Galli
authors: Agostino
authors: A
authors: Riva
mesh_terms: D016915:AIDS Vaccines / Q000276:immunology; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018450:Disease Progression; D005260:Female; D005434:Flow Cytometry; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D019409:Interleukin-15 / Q000378:metabolism*; D053720:Interleukin-15 Receptor alpha Subunit / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D010641:Phenotype
chemical_list: D016915:AIDS Vaccines; D019409:Interleukin-15; D053720:Interleukin-15 Receptor alpha Subunit; D018950:Lipopolysaccharide Receptors