title: Two-dimensional difference gel electrophoresis applied for analytical proteomics: fundamentals and applications to the study of plant proteomics.
abstract: The present review reports the principles, fundamentals and some applications of two-dimensional difference gel electrophoresis for analytical proteomics based on plant proteome analysis, also emphasizing some advantages of 2-D DIGE over 2-D PAGE techniques. Some fluorescent protein labeling reagents, methods of protein labeling, models of 2-D DIGE experiments, and some limitations of this technique are presented and discussed in terms of 2-D DIGE plant proteomes. Finally, some practical applications of this technique are pointed out, emphasizing its potentialities in plant proteomics.
authors: Laboratory of Genetics and Biochemistry of Plants, Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz, ESALQ, USP, 13400-970 Piracicaba, SP, Brazil.
authors: Sandra Cristina Capaldi
authors: SC
authors: Arruda
authors: Herbert de Sousa
authors: Hde S
authors: Barbosa
authors: Ricardo Antunes
authors: RA
authors: Azevedo
authors: Marco Aurélio Zezzi
authors: MA
authors: Arruda
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D005456:Fluorescent Dyes / Q000737:chemistry; D010944:Plants / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D020543:Proteome