title: Adeno-associated virus-mediated local delivery of LIGHT suppresses tumorigenesis in a murine cervical cancer model.
abstract: LIGHT is a tumor necrosis factor superfamily ligand that is considered as a promising candidate for cancer therapy. It has a potent antitumor activity through establishing lymphoid-like tissues inside tumor sites and recruiting naive T cells into the tumor. In this study, we examined the possibility of antitumor activity by expressing LIGHT in cervical cancer (CC) model. A recombinant adeno-associated virus (AAV) vector was chosen for the transfer, based on its transfection efficiency and lack of detectable pathology. In vitro transfer of recombinant AAV vector expressing LIGHT (AAV-LIGHT) stimulated T-lymphocyte proliferation and activation. AAV-mediated gene transfer of LIGHT by intratumoral injection exerted a very potent antitumor effect against preexisting TC-1 cell CC in C57BL/6 mice. This study confirmed that AAV-LIGHT regressed tumor growth by activating cytotoxic T lymphocyte, enhancing infiltration of inflammatory cells in tumor and increasing stimulatory cytokine expression in tumor microenvironment. Therefore, AAV-LIGHT therapy might have potential utility for the treatment of CC.
authors: Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
authors: Mayinuer
authors: M
authors: Maitituoheti
authors: Yan
authors: Y
authors: Li
authors: Wenwen
authors: W
authors: Wang
authors: Wei
authors: W
authors: Wang
authors: Lingfei
authors: L
authors: Han
authors: Runfeng
authors: R
authors: Yang
authors: Tian
authors: T
authors: Wang
authors: Zhangying
authors: Z
authors: Wu
authors: Ding
authors: D
authors: Ma
authors: Shixuan
authors: S
authors: Wang
mesh_terms: D000818:Animals; D016466:CHO Cells; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D000229:Dependovirus / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D014161:Transduction, Genetic; D047368:Tumor Burden / Q000235:genetics / Q000276:immunology; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000628:therapy*; D023041:Xenograft Model Antitumor Assays
chemical_list: D053326:Tumor Necrosis Factor Ligand Superfamily Member 14