title: Determination of hexanal as an oxidative marker in vegetable oils using an automated dynamic headspace sampler coupled to a gas chromatograph/mass spectrometer.
abstract: An automated dynamic headspace sampler coupled to a gas chromatograph/mass spectrometer was evaluated as an oxidative marker to determine hexanal content in vegetable oils. For the effective analysis, a cooled injection system (CIS) was used to focus and to introduce the hexanal desorbed from the Tenax TA. The temperature of the CIS was maintained at -60 °C for 12 min before desorbing the hexanal. Hexanal was separated on a capillary column (DB-5, 0.25 mm × 60 m, 0.25 µm in film thickness) from 50 to 230 °C, followed by mass spectrometer-selected ion monitoring analysis at m/z 56. The instrumental response to hexanal was highly linear from 10 ng mL(-1) to 1 µg mL(-1) (r(2) = 0.9999). The relative standard deviation (RSD) of intra- and inter-day repeatability was acceptable, with values of less than 3.88 and 4.25%, respectively. The LOD and LOQ of hexanal were determined by gas chromatograph/mass spectrometer-selected ion monitoring to be 3.3 and 9.8 ng mL(-1), respectively. The acid value, peroxide value and fatty acid composition revealed a good correlation with the hexanal concentration.
authors: Food Analysis Center, Korea Food Research Institute, 516, Baekhyeon, Bundang, Seongnam, Gyeonggi, 463-746, Republic of Korea. jhkfri@kfri.re.kr
authors: Jaeho
authors: J
authors: Ha
authors: Dong-Won
authors: DW
authors: Seo
authors: Xi
authors: X
authors: Chen
authors: Jin-Bong
authors: JB
authors: Hwang
authors: You-Shin
authors: YS
authors: Shim
mesh_terms: D000447:Aldehydes / Q000032:analysis*; D057205:Automation, Laboratory / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry* / Q000145:classification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D000447:Aldehydes; D010938:Plant Oils; C010463:n-hexanal