title: Three-dimensional strain fields in human brain resulting from formalin fixation.
abstract: Before investigating human brains post mortem, the first preparation step is often formalin fixation of the brain. As the brain consists of inhomogeneous tissues, the fixation leads to a three-dimensional strain field within the tissue. During the single case MR-based investigation of the brain, first, the starting point with the brain post mortem but still within the cranium, was examined. Then 13 MR data sets were acquired over a fixation period of 70 days and compared to the initial data set. Based on affine registration of the data sets, the global volume shrinkage was found to be 8.1%. By means of a non-rigid registration additional maximal local volume strains of 32% were determined.
authors: Biomaterials Science Center, University of Basel, c/o University Hospital Basel, 4031 Basel, Switzerland. georg.schulz@unibas.ch
authors: Georg
authors: G
authors: Schulz
authors: Hendrikus J A
authors: HJ
authors: Crooijmans
authors: Marco
authors: M
authors: Germann
authors: Klaus
authors: K
authors: Scheffler
authors: Magdalena
authors: M
authors: Müller-Gerbl
authors: Bert
authors: B
authors: Müller
mesh_terms: D000368:Aged; D000465:Algorithms; D016477:Artifacts; D001921:Brain / Q000033:anatomy & histology*; D003432:Cross-Linking Reagents; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging; D008297:Male; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D005404:Fixatives; D005557:Formaldehyde