title: Effect of supplementation of soft drinks with green tea extract on their erosive potential against dentine.
abstract: BACKGROUND
  Matrix metalloproteinase (MMP) inhibitors reduce dentine erosion. This in vitro study evaluated the effect of the supplementation of soft drinks with green tea extract, a natural inhibitor of MMPs, on their erosive potential against dentine.
  
  
  METHODS
  For each drink tested (Coca-Cola, Kuat guarana, Sprite and light Coca-Cola), 40 dentine specimens were divided into two subgroups differing with respect to supplementation with green tea extract at 1.2% (OM24, 100%Camellia sinensis leaf extract, containing 30 ± 3% of catechin; Omnimedica, Switzerland) or not (control). Specimens were subjected to four pH cycles, alternating de- and remineralization in one day. For each cycle, samples were immersed in pure or supplemented drink (10 minutes, 30 mL per block) and in artificial saliva (60 minutes, 30 mL per block) at 37 °C, under agitation. Dentine alterations were determined by profilometry (μm). Data were analysed by two-way ANOVA and Bonferroni's test (p < 0.05).
  
  
  RESULTS
  A significant difference was observed among the drinks tested with Sprite leading to the highest surface loss and light Coca-Cola to the lowest. Supplementation with green tea extract reduced the surface loss by 15% to 40% but the difference was significant for Coca-Cola only.
  
  
  CONCLUSIONS
  Supplementation of soft drinks with green tea extract might be a viable alternative to reduce their erosive potential against dentine.
authors: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, Brazil.
authors: C S
authors: CS
authors: Barbosa
authors: M T
authors: MT
authors: Kato
authors: M A R
authors: MA
authors: Buzalaf
mesh_terms: D000818:Animals; D028241:Camellia sinensis; D002253:Carbonated Beverages / Q000009:adverse effects; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002417:Cattle; D003804:Dentin / Q000187:drug effects* / Q000473:pathology; D019587:Dietary Supplements; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts / Q000008:administration & dosage*; D012464:Saliva, Artificial / Q000008:administration & dosage; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000517:prevention & control; D014077:Tooth Erosion / Q000517:prevention & control*; D014089:Tooth Remineralization
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D010936:Plant Extracts; D012464:Saliva, Artificial; D002392:Catechin; C045651:epigallocatechin gallate