title: PTZ-induced seizures inhibit adenosine deamination in adult zebrafish brain membranes.
abstract: Adenosine exerts neuromodulatory functions with mostly inhibitory effects, being considered an endogenous anticonvulsant. The hydrolysis of ATP by ectonucleotidases is an important source of adenosine, and adenosine deaminase (ADA) contributes to the regulation of this nucleoside concentration through its deamination. In this study, we tested the effect of pentylenetetrazole (PTZ)-induced seizures on ectonucleotidase and ADA activities in adult zebrafish brain. Our results have demonstrated that PTZ treatments did not alter ectonucleotidase and ADA activities in membranes and soluble fraction, respectively. However, ecto-ADA activity was significantly decreased in brain membranes of animals exposed to 5mM and 15 mM PTZ treatments (22.4% and 29.5%, respectively) when compared to the control group. Semiquantitative RT-PCR analysis did not show significant changes after the PTZ exposure on ADA gene expression. The decreased adenosine deamination observed in this study suggests a modulation of extracellular adenosine levels during PTZ-induced seizures in zebrafish.
authors: Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Programa de Pós-Graduação em Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga, 6681, 90619-900 Porto Alegre, RS, Brazil.
authors: Anna Maria
authors: AM
authors: Siebel
authors: Angelo Luis
authors: AL
authors: Piato
authors: Katiucia Marques
authors: KM
authors: Capiotti
authors: Kelly Juliana
authors: KJ
authors: Seibt
authors: Maurício Reis
authors: MR
authors: Bogo
authors: Carla Denise
authors: CD
authors: Bonan
mesh_terms: D000241:Adenosine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D003292:Convulsants / Q000494:pharmacology*; D003641:Deamination; D010433:Pentylenetetrazole / Q000494:pharmacology*; D011755:Pyrophosphatases / Q000378:metabolism; D012640:Seizures / Q000139:chemically induced* / Q000503:physiopathology*; D015027:Zebrafish
chemical_list: D003292:Convulsants; D000243:Adenosine Deaminase; D011755:Pyrophosphatases; D000241:Adenosine; D010433:Pentylenetetrazole