title: Comparison of peptide cancer signatures identified by mass spectrometry in serum of patients with head and neck, lung and colorectal cancers: association with tumor progression.
abstract: Mass spectrometry-based analyses of the low-molecular-weight fraction of             serum proteome allow identifying proteome profiles (signatures) that are potentially             useful in detection and diagnostics of cancer. Here we compared serum proteome             profiles of healthy donors and patients with three different types of cancer aiming             to identify peptide signatures that were either common for all cancer samples             or specific for cancer type. Blood samples were collected before start of the             therapy from patients with head and neck squamous cell cancer, colorectal adenocarcinoma             and non-small cell lung cancer, and from a corresponding group of healthy volunteers.             Mass profiles of the serum proteome were recorded in the range between 2 and 13             kDa using MALDI-ToF spectrometry and 131 identified peptide ions were used for             statistical analyses. Similar degrees of overall similarities were observed in             all intra-group and inter-group analyses when general features of serum proteome             profiles were compared between individual samples. However, classifiers built             of selected spectral components allowed differentiation between healthy donors             and three groups of cancer patients with 69-74% sensitivity and 82-84% specificity.             There were two common peptide species (3766 and 5867 Da) with increased levels             in all cancer samples. Several spectral components permitted differentiation between             lung cancer samples and either head and neck cancer or colorectal cancer samples,             but two latter types of samples could not be properly discriminated. Abundance             of spectral components that putatively corresponded to fragments of serum amyloid             A (11511 and 11667 Da) was highest in lung cancer samples, yet increased levels             of these peptides appeared to generally associate with more advanced cancer cases.             We concluded that certain components of serum peptide signatures are common for             different cancer signatures and putatively reflect general response of organism             to the disease, yet other components of such signatures are more specific and             most likely correspond to clinical stage of the malignancy.
authors: Maria Skłodowska-Curie Memorial Cancer Center and Institute of                     Oncology, Gliwice, Poland.
authors: Monika
authors: M
authors: Pietrowska
authors: Joanna
authors: J
authors: Polańska
authors: Rafał
authors: R
authors: Suwiński
authors: Maciej
authors: M
authors: Wideł
authors: Tomasz
authors: T
authors: Rutkowski
authors: Michał
authors: M
authors: Marczyk
authors: Iwona
authors: I
authors: Domińczyk
authors: Lucyna
authors: L
authors: Ponge
authors: Lukasz
authors: L
authors: Marczak
authors: Andrzej
authors: A
authors: Polański
authors: Piotr
authors: P
authors: Widłak
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000473:pathology; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000097:blood* / Q000473:pathology; D018450:Disease Progression; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000473:pathology; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood*; D010446:Peptide Fragments / Q000097:blood*; D020543:Proteome / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D009363:Neoplasm Proteins; D010446:Peptide Fragments; D020543:Proteome