title: Detection of a bacteriophage gene encoding a Mu-like portal protein in Haemophilus parasuis reference strains and field isolates by nested polymerase chain reaction.
abstract: A nested polymerase chain reaction (nPCR) assay was developed to determine the presence of a gene encoding a bacteriophage Mu-like portal protein, gp29, in 15 reference strains and 31 field isolates of Haemophilus parasuis. Specific primers, based on the gene's sequence, were utilized. A majority of the virulent reference strains and field isolates tested harbored the gene. The results suggest that the nPCR technique described in the current report could serve as a tool for epidemiological studies of H. parasuis.
authors: Food Safety and Enteric Pathogens Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, 1920 Dayton Avenue, Building 24, Ames, IA 50010, USA. emilie.zehr@ars.usda.gov
authors: Emilie S
authors: ES
authors: Zehr
authors: Louisa B
authors: LB
authors: Tabatabai
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D005814:Genes, Viral / Q000235:genetics*; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary / Q000821:virology; D044137:Haemophilus parasuis / Q000302:isolation & purification / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D013552:Swine