title: An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle.
abstract: Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
authors: Biotechnology Institute, Veterinary and Agronomic Sciences Research Center (CICVyA), National Institute of Agricultural Technology (INTA), Castelar, Buenos Aires, Argentina.
authors: Silvina Elizabeth
authors: SE
authors: Wilkowsky
authors: Guillermo Gimenez
authors: GG
authors: Bareiro
authors: María Laura
authors: ML
authors: Mon
authors: Dadin Prando
authors: DP
authors: Moore
authors: Gastón
authors: G
authors: Caspe
authors: Carlos
authors: C
authors: Campero
authors: Marcelo
authors: M
authors: Fort
authors: María Isabel
authors: MI
authors: Romano
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*; D003048:Coccidiosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D018688:Neospora / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C115390:SAG1 protein, Neospora caninum