title: Rab5A is associated with axillary lymph node metastasis in breast cancer patients.
abstract: The expression of Rab proteins has been associated with cancer. However, few data are available on Rab5A expression in human breast cancer or its impact on disease progression. First, we examined the functional role of Rab5A in breast cancer cells. The expression of Rab5A in MDA-MB-231 cells can be stimulated by epidermal growth factor in a dose-dependent manner. The epidermal growth factor-induced increase of Rab5A expression correlated well with enhanced migration in wound healing migration assays in these cells. Furthermore, we evaluated the expression of Rab5A in breast cancer specimens using immunohistochemical staining, then analyzed the relationship between the expression of Rab5A and clinicopathological parameters. The increased expression of Rab5A protein in 123 breast cancer samples was associated with higher histological grade (P = 0.004), more lymphovascular invasion (P = 0.027), more axillary lymph node (LN) metastasis (P = 0.008), and a higher number of axillary LN metastases (P = 0.043). Among 218 axillary LNs of more than 10 breast cancer patients with node metastases, 167 metastatic LNs were found to have increased Rab5A expression. Rab5A is associated with axillary LN metastasis in breast cancer patients.
authors: Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.
authors: Po-Sheng
authors: PS
authors: Yang
authors: Pen-Hui
authors: PH
authors: Yin
authors: Ling-Ming
authors: LM
authors: Tseng
authors: Chin-Hua
authors: CH
authors: Yang
authors: Chih-Yi
authors: CY
authors: Hsu
authors: Ming-Yuan
authors: MY
authors: Lee
authors: Cheng-Fang
authors: CF
authors: Horng
authors: Chin-Wen
authors: CW
authors: Chi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D018450:Disease Progression; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology*; D008875:Middle Aged; D020696:rab5 GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D004815:Epidermal Growth Factor; D020696:rab5 GTP-Binding Proteins