title: Anti-angiogenic effects of water extract of a formula consisting of Pulsatilla koreana, Panax ginseng and Glycyrrhiza uralensis.
abstract: OBJECTIVE
  This study aimed to investigate the anti-angiogenic effects of the water extract of Pulsatilla koreana (Yabe ex Nakai) Nakai ex T. Mori., Panax ginseng C.A. Meyer and Glycyrrhiza uralensis Fisch (WEPPG).
  
  
  METHODS
  The effects of WEPPG on fibroblast growth factor (bFGF)-induced angiogenesis were evaluated by human umbilical vein endothelial cell (HUVEC) proliferation, adhesion, and migration assays. Capillary tube formation of HUVECs and bFGF-induced chick chorioallantoic membrane (CAM) angiogenesis were also observed. WEPPG was used to treat the HUVECs and CAMs, and then various activities such as proliferation, adhesion, migration, capillary tube formation and cell cycle proteins were analyzed.
  
  
  RESULTS
  WEPPG significantly inhibited bFGF-induced HUVEC proliferation, adhesion, migration, and capillary tube formation. Signaling protein analysis showed up-regulated expressions of various proteins including cyclin A, p63 and KIP2 and down-regulated expressions of nibrin and focal adhesion kinase. The blood vessel formation in a CAM treated with WEPPG was markedly reduced compared with the control group.
  
  
  CONCLUSIONS
  These results suggested that the inhibition of angiogenesis by WEPPG can be an action mechanism for its anti-cancer effects.
authors: East-West Cancer Center, Dunsan Oriental Medical Hospital of Daejeon University, Daejeon, Korea.
authors: Jong-Min
authors: JM
authors: Kim
authors: Kyung-Soon
authors: KS
authors: Kim
authors: Yeon-Weol
authors: YW
authors: Lee
authors: Chong-Kwan
authors: CK
authors: Cho
authors: Hwa-Seung
authors: HS
authors: Yoo
authors: Ji-Young
authors: JY
authors: Bang
authors: Eung-Yoon
authors: EY
authors: Kim
authors: In-Cheol
authors: IC
authors: Kang
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D029882:Glycyrrhiza uralensis / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D005894:Panax / Q000737:chemistry*; D031946:Pulsatilla / Q000737:chemistry*
chemical_list: D043925:Angiogenesis Inducing Agents; D004365:Drugs, Chinese Herbal