title: Pilot study of the application of magnetic bead protein profiling to the study of biomarkers in addiction research.
abstract: OBJECTIVE
  Proteomic technologies based on mass spectrometry are increasingly used as a valuable tool in clinical research allowing high-throughput protein and peptide profiling to be undertaken. Whilst previous research has focussed the application of this novel technology on the study of patients with disorders compared to comparable individuals from the healthy population, this current study seeks to determine the effect of successful treatment for alcoholism on the serum protein profile obtained.
  
  
  METHODS
  Serum samples were collected from patients after initial treatment for alcohol abuse and also 6 months after treatment. The serum samples were prepared for analysis using reverse phase magnetic bead fractionation and the resulting peptides analysed by matrix assisted laser desorption ionisation time-of-flight (MALDI-ToF) mass spectrometry.
  
  
  RESULTS
  Whilst the majority of the peptides detected by this approach exhibited constant levels between the two time points, three peptides were elevated at the 6-month time point compared to the initial sampling.
  
  
  CONCLUSIONS
  Whilst disorders with very clear biological causes (such as cancer) exhibit significantly different peptide profiles, psychiatric disorders such as alcohol addiction which are multifactorial show less obvious changes. Despite this the two groups of samples could statistically be distinguished by certain peptides expression levels.
authors: Division of Substance Use Disorders, Psychiatric Hospital of University of Basel, Basel, Switzerland.
authors: L
authors: L
authors: Meister
authors: K
authors: K
authors: Alawam
authors: E
authors: E
authors: Dudley
authors: R
authors: R
authors: Taurines
authors: S E
authors: SE
authors: Müller
authors: M
authors: M
authors: Walter
authors: J
authors: J
authors: Höppner
authors: S
authors: S
authors: Teipel
authors: R M
authors: RM
authors: Donev
authors: A
authors: A
authors: Eckert
authors: G A
authors: GA
authors: Wiesbeck
authors: J
authors: J
authors: Thome
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000097:blood* / Q000534:rehabilitation; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D008280:Magnetics; D008297:Male; D010455:Peptides / Q000097:blood; D010865:Pilot Projects; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D016540:Smoking Cessation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D016320:Substance Abuse Treatment Centers; D014029:Tobacco Use Disorder / Q000097:blood* / Q000534:rehabilitation; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D010455:Peptides