title: In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.).
abstract: Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10 μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-D-glucosyl (1→4)-β-D-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1 μg/ml (IC(50)=0.86 μg/ml).
authors: Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
authors: Hiroaki
authors: H
authors: Kiyohara
authors: Chikara
authors: C
authors: Ichino
authors: Yuka
authors: Y
authors: Kawamura
authors: Takayuki
authors: T
authors: Nagai
authors: Noriko
authors: N
authors: Sato
authors: Haruki
authors: H
authors: Yamada
authors: Maha M
authors: MM
authors: Salama
authors: Essam
authors: E
authors: Abdel-Sattar
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D002298:Cardenolides / Q000494:pharmacology; D002301:Cardiac Glycosides / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002460:Cell Line; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D008756:Methylgalactosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012529:Saudi Arabia; D019562:Viral Load / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D002298:Cardenolides; D002301:Cardiac Glycosides; D002316:Cardiotonic Agents; D008756:Methylgalactosides; D010936:Plant Extracts; C571267:oleandrigenin-beta-D-glucosyl (1-4)-beta-D-digitalose; C000964:digitalose; D000432:Methanol