title: Growth and shortening of microtubules: a two-state model approach.
abstract: In this study, a two-state mechanochemical model is presented to describe the dynamic instability of microtubules (MTs) in cells. The MT switches between two states, the assembly and disassembly states. In assembly state, the growth of MTs includes two processes: free GTP-tubulin binding to the tip of protofilament (PF) and conformation change of PF, during which the first tubulin unit that curls outwards is rearranged onto the MT surface, using the energy released from the hydrolysis of GTP in the penultimate tubulin unit. In the disassembly state, the shortening of MTs also includes two processes, the release of GDP-tubulin from the tip of PF and the curling of one new tubulin unit out of the MT surface. Switches between these two states, which are usually called rescue and catastrophe, happen stochastically with external force-dependent rates. Using this two-state model with parameters obtained by fitting the recent experimental data, detailed properties of MT growth are obtained. I find that MT is mainly in the assembly state, its mean growth velocity increases with both the external force and the GTP-tubulin concentration, and an MT will shorten on average without an external force. To know more about the external force and GTP-tubulin concentration-dependent properties of MT growth, and for future experimental verification of this two-state model, 11 critical forces are defined and discussed numerically.
authors: Shanghai Key Laboratory for Contemporary Applied Mathematics, Centre for Computational Systems Biology, School of Mathematical Sciences, Fudan University, Shanghai 200433, China. xyz@fudan.edu.cn
authors: Yunxin
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D006160:Guanosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014404:Tubulin; D006160:Guanosine Triphosphate