title: Measurement of 3-hydroxyisovaleric acid in urine from marginally biotin-deficient humans by UPLC-MS/MS.
abstract: Measurement of 3-hydroxyisovaleric acid (3HIA) in human urine has been shown to be a useful indicator of biotin status for a variety of clinical situations, including pregnancy. The work described herein presents a novel UPLC-MS/MS method for accurate and precise quantitation of urinary 3HIA. This method utilizes sample preparation prior to quantitation that has been simplified compared to the previous GC-MS method. To demonstrate the suitability of the UPLC-MS/MS method for human bio-monitoring, this method was used to measure 3-HIA in 64 human urine samples from eight healthy adults in whom marginal biotin deficiency had been induced experimentally by egg white feeding. 3HIA was detected in all specimens; the mean concentration [±standard deviation (SD)] was 80.6 ± 51 μM prior to inducing biotin deficiency. Mean excretion rate for 3HIA (expressed per mol urinary creatinine) before beginning the biotin-deficient diet was 8.5 ± 3.2 mmol 3HIA per mol creatinine and the mean increased threefold with deficiency. These specimens had been previously analyzed by GC-MS; the two data sets showed strong linear relationship with a correlation coefficient of 0.97. These results provide evidence that this method is suitable for bio-monitoring of biotin status in larger populations.
authors: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors: Thomas D
authors: TD
authors: Horvath
authors: Nell I
authors: NI
authors: Matthews
authors: Shawna L
authors: SL
authors: Stratton
authors: Donald M
authors: DM
authors: Mock
authors: Gunnar
authors: G
authors: Boysen
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine*; D001710:Biotin / Q000172:deficiency / Q000652:urine*; D028921:Biotinidase Deficiency / Q000139:chemically induced / Q000652:urine*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D004529:Egg White / Q000009:adverse effects*; D005260:Female; D006801:Humans; D008297:Male; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014631:Valerates / Q000652:urine*
chemical_list: D015415:Biomarkers; D014631:Valerates; C004961:beta-hydroxyisovaleric acid; D001710:Biotin; D003404:Creatinine