title: The Protein Maker: an automated system for high-throughput parallel purification.
abstract: The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications.
authors: Seattle Structural Genomics Center for Infectious Disease (http://www.ssgcid.org), USA.
authors: Eric R
authors: ER
authors: Smith
authors: Darren W
authors: DW
authors: Begley
authors: Vanessa
authors: V
authors: Anderson
authors: Amy C
authors: AC
authors: Raymond
authors: Taryn E
authors: TE
authors: Haffner
authors: John I
authors: JI
authors: Robinson
authors: Thomas E
authors: TE
authors: Edwards
authors: Natalie
authors: N
authors: Duncan
authors: Cory J
authors: CJ
authors: Gerdts
authors: Mark B
authors: MB
authors: Mixon
authors: Peter
authors: P
authors: Nollert
authors: Bart L
authors: BL
authors: Staker
authors: Lance J
authors: LJ
authors: Stewart
mesh_terms: D001331:Automation / Q000295:instrumentation* / Q000379:methods; D003045:Coccidioides / Q000737:chemistry* / Q000201:enzymology; D058886:Sterol 14-Demethylase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D058886:Sterol 14-Demethylase