title: Apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and kit.
abstract: In the present study, apoptotic, antioxidant and antiradical effects of majdine and isomajdine from Vinca herbacea Waldst. and Kit were studied. For testing the possible apoptotic effects of majdine and isomajdine from V. herbacea, DNA fragmentation assay was conducted on the rat brain cortical tissue homogenates, in vitro. Also their possible effects on mitochondrial activity were tested by using the same tissue samples of rats. In addition, the antioxidant activity of isomajdine and majdine was determined using various in vitro antioxidant assays, including 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS(•+)) radical scavenging and N,N-dimethyl-p-phenylenediamine (DMPD(•+)) radical scavenging, ferric ions (Fe(3+)) and cupric ions (Cu(2+)) reducing abilities and ferrous ions (Fe(2+)) chelating activity. On the other hand, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) were used as reference antioxidants.
authors: Faculty of Sciences, Department of Chemistry, Atatürk University, Erzurum, Turkey. igulcin@atauni.edu.tr
authors: Ilhami
authors: I
authors: Gülçin
authors: Sükrü
authors: S
authors: Beydemir
authors: Fevzi
authors: F
authors: Topal
authors: Natia
authors: N
authors: Gagua
authors: Aliko
authors: A
authors: Bakuridze
authors: Recep
authors: R
authors: Bayram
authors: Akçahan
authors: A
authors: Gepdiremen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000737:chemistry; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D010084:Oxidation-Reduction; D000078183:Oxindoles; D051381:Rats; D017207:Rats, Sprague-Dawley; D013329:Structure-Activity Relationship; D014748:Vinca Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D000078183:Oxindoles; D014748:Vinca Alkaloids; C571794:majdine