title: Detection of a fatty liver after binge drinking: correlation of MR-spectroscopy, DECT, biochemistry and histology in a rat model.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the possibility of detecting a fatty liver after binge drinking in an animal model using (1)H magnetic resonance spectroscopy ((1)H-MRS), dual-energy computed tomography (DECT), biochemistry, and the gold standard of histology.
  
  
  METHODS
  In 20 inbred female Lewis rats, an alcoholic fatty liver was induced; 20 rats served as controls. To simulate binge drinking, each rat was given a dose of 9.3 g/kg body weight 50% ethanol twice, with 24 hours between applications. Forty-eight hours after the first injection, DECT and (1)H-MRS were performed. Fat content as well as triglycerides were also determined histologically and biochemically, respectively. To assess specific liver enzymes, blood was drawn from the orbital venous plexus.
  
  
  RESULTS
  In all 20 animals in the experimental group, fatty livers were detected using (1)H-MRS, DECT, and biochemical and histologic analysis. The spectroscopic fat/water ratio and the biochemical determination were highly correlated (r = 0.892, P < .05). A significant correlation was found between (1)H-MRS and histologic analysis (r = 0.941, P < .001). Also, a positive linear correlation was found between the dual-energy computed tomographic density of ΔHU and the biochemical (r = 0.751, P < .05) and histologic (r = 0.786, P < .001) analyses.
  
  
  CONCLUSIONS
  Quantification of hepatic fat content on (1)H-MRS showed high correlation with histologic and biochemical steatosis determination. In comparison to DECT, it is more suitable to reflect the severity of acute fatty liver.
authors: Department of Diagnostic and Interventional Radiology, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany. huedayi.korkusuz@kgu.de
authors: Huedayi
authors: H
authors: Korkusuz
authors: Daniel
authors: D
authors: Keese
authors: Bahram Abbas
authors: BA
authors: Raschidi
authors: Frank
authors: F
authors: Hübner
authors: Dmitry
authors: D
authors: Namgaladze
authors: Gudrun
authors: G
authors: Hintereder
authors: Renate
authors: R
authors: Hammerstingl
authors: Yuecel
authors: Y
authors: Korkusuz
authors: Christian
authors: C
authors: Mönch
authors: Thomas J
authors: TJ
authors: Vogl
mesh_terms: D000428:Alcohol Drinking; D000818:Animals; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000175:diagnosis*; D005260:Female; D016014:Linear Models; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D011917:Rats, Inbred Lew; D014057:Tomography, X-Ray Computed / Q000379:methods*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D014280:Triglycerides