title: Two systemic lupus erythematosus (SLE) global disease activity indexes--the SLE Disease Activity Index and the Systemic Lupus Activity Measure--demonstrate different correlations with activation of peripheral blood CD4+ T cells.
abstract: Global disease activity measurement in systemic lupus erythematosus (SLE) patients is important for the clinical estimation and adjustment of therapy. By contrast, immune system activation plays a significant role in disease pathogenesis, with CD4+ lymphocytes acting as central cells in the immune response. We investigated which scale better correlates with immunologic changes in the blood of SLE patients, the SLE Disease Activity Index (SLEDAI) or the Systemic Lupus Activity Measure (SLAM) scale. Samples of peripheral blood were obtained from 45 SLE patients with different disease activity as assessed by the SLEDAI and the SLAM scales on the same day. We assessed the percentage of CD4+ T cells with activation-associated receptors: CD69, CD25int, CD95, HLA-DR, and CD4+ T cells with killing properties containing perforin and granzyme B. Our results indicated that the percentage of CD4+CD69+ and CD4+CD25(int) cells did not correlate with either the SLEDAI or the SLAM scale. Significant and positive correlations were observed between percentages of CD4+CD95+ and CD4+HLA-DR+ lymphocytes and SLE activity, but only when activity was measured using the SLAM scale, not with the SLEDAI scale. The percentage of CD4+perforin+ and CD4+granzyme B+ cells also strongly correlated with disease activity measured only with the SLAM scale. We conclude that the SLAM scale better reflects changes of immune system activity in SLE patients compared with the SLEDAI scale.
authors: Department of Pathophysiology, Medical University of Gdańsk, Gdańsk, Poland.
authors: Agnieszka
authors: A
authors: Daca
authors: Zenobia
authors: Z
authors: Czuszyńska
authors: Zaneta
authors: Z
authors: Smoleńska
authors: Zbigniew
authors: Z
authors: Zdrojewski
authors: Jacek M
authors: JM
authors: Witkowski
authors: Ewa
authors: E
authors: Bryl
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D053804:Granzymes / Q000032:analysis / Q000096:biosynthesis; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D054353:Perforin / Q000032:analysis / Q000096:biosynthesis; D011044:Poland; D012107:Research Design; D012720:Severity of Illness Index; D013223:Statistics as Topic
chemical_list: D015703:Antigens, CD; D006684:HLA-DR Antigens; D054353:Perforin; D053804:Granzymes