title: Comparative pharmacokinetics of three marker compounds in mBHT and single-herb extract after oral administration to rats.
abstract: Modified Bo-Yang-Hwan-O-Tang (mBHT) is a decoction of 12 herbs traditionally used in the treatment of cerebral and cardiac stroke and vascular dementia. Paeoniflorin (PF), calycosin-7-O-β-D-glycoside (CY), and salvianolic acid B (SB) are marker compounds for extracts of the herbs Paeoniae Radix, Astragali Radix, and Salviae Miltiorrhizae Radix, respectively, and are used to assess the quality of mBHT. This study examined the pharmacokinetics of these three marker compounds following oral administration of each herb extract alone and in combination as mBHT in rats. The concentrations of the three compounds in rat plasma were determined by high-performance liquid chromatography, using a C18 column (2.1×150 mm, 5 μm) and mobile phases of methanol-water-formic acid (10:90:0.05, v/v) and methanol-water (90:10, v/v). The results indicated that the pharmacokinetic parameters of Paeoniae Radix extract group and mBHT group were very similar, while those of Salviae Miltiorrhizae Radix extract group and mBHT group were significantly different (P<0.05, t-test). The T(max), AUC and T(1/2) of SB for Salviae Miltiorrhizae Radix extract group were 54.7 min, 598.7 μg min/ml and 37.4 min, respectively. However, these values increased to 77.6 min, 915.9 μg min/ml and 53.7 min for mBHT group, supposing that excretion of SB could be more retarded when administered in mBHT than in Salviae Miltiorrhizae Radix extract.
authors: College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
authors: Jianbo
authors: J
authors: Chen
authors: Yan
authors: Y
authors: Ding
authors: Xitao
authors: X
authors: Yan
authors: Kwan Jun
authors: KJ
authors: Lee
authors: Young Ho
authors: YH
authors: Kim
authors: Cheong Weon
authors: CW
authors: Cho
authors: Yong Ki
authors: YK
authors: Park
authors: Wonjae
authors: W
authors: Lee
authors: Jong Seong
authors: JS
authors: Kang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D015415:Biomarkers / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D010936:Plant Extracts; C479639:bo-yang-hwan-o-tang