title: Porcine circovirus genotype 2a (PCV2a) and genotype 2b (PCV2b) recombinant mutants showed significantly enhanced viral replication and altered antigenicity in vitro.
abstract: Two recombinant mutants of porcine circovirus type 2 (PCV2), which resulted from replacement of a genomic fragment containing the open reading frame 2 (ORF2) of genotype PCV2b with that of genotype PCV2a, were obtained initially from co-infection with PCV2a and 2b genotype viruses in vitro. The two mutant viruses contained the ORF1 sequence from genotype PCV2b and the ORF2 sequence from genotype PCV2a. They were designated according to the nomenclature proposed by Grau et al., indicating the origin of the ORF1 sequence first and that of the ORF2 sequence second, i.e., PCV2b(JF11)/2a(CL1) and PCV2b(YJ)/2a(CL1). The replication efficiencies of the two PCV2 recombinant mutants were enhanced significantly and their antigenicities were altered significantly in vitro when compared with their parental strains.
authors: Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors: Longjun
authors: L
authors: Guo
authors: Yuehua
authors: Y
authors: Lu
authors: Yanwu
authors: Y
authors: Wei
authors: Liping
authors: L
authors: Huang
authors: Hongli
authors: H
authors: Wu
authors: Changming
authors: C
authors: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D008854:Microscopy, Electron; D009154:Mutation; D016366:Open Reading Frames; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D014779:Virus Replication / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004279:DNA, Viral