title: Bacterial outer membrane channel for divalent metal ion acquisition.
abstract: The prevailing model of bacterial membrane function predicts that the outer membrane is permeable to most small solutes because of pores with limited selectivity based primarily on size. Here, we identified mnoP in the Gram-negative bacterium Bradyrhizobium japonicum as a gene coregulated with the inner membrane Mn(2+) transporter gene mntH. MnoP is an outer membrane protein expressed specifically under manganese limitation. MnoP acts as a channel to facilitate the tranlocation of Mn(2+), but not Co(2+) or Cu(2+), into reconstituted proteoliposomes. An mnoP mutant is defective in high-affinity Mn(2+) transport into cells and has a severe growth phenotype under manganese limitation. We suggest that the outer membrane is a barrier to divalent metal ions that requires a selective channel to meet the nutritional needs of the cell.
authors: Department of Biochemistry, State University of New York, Buffalo, NY 14214, USA.
authors: Thomas H
authors: TH
authors: Hohle
authors: William L
authors: WL
authors: Franck
authors: Gary
authors: G
authors: Stacey
authors: Mark R
authors: MR
authors: O'Brian
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D020369:Bradyrhizobium / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008345:Manganese / Q000172:deficiency / Q000378:metabolism / Q000494:pharmacology; D008670:Metals / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D017433:Protein Structure, Secondary
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D002413:Cations, Divalent; D007473:Ion Channels; D008081:Liposomes; D008670:Metals; C433793:MntH protein, bacteria; D008345:Manganese