title: Identification of seed dormancy mutants by activation tagging.
abstract: Activation tagging is an important tool for gene discovery in plants. This method utilizes a T-DNA sequence that contains four tandem copies of the cauliflower mosaic virus 35S enhancer sequence or promoters oriented outward to the T-DNA border sequences. These elements enhance the expression of genes neighboring on either side of the randomly integrated T-DNA, resulting in gain-of-function phenotypes. Activation tagging has identified a number of genes, including those fundamental to plant development, such as the floral inducer gene, FLOWERING LOCUS T  (FT  ). The methods surrounding activation-tagging approaches are described in this chapter. While seeds have generally not been the targets of these methods in the past, activation tagging provides a powerful approach to uncover genes involved in seed dormancy and germination, including those that mediate hormone signal transduction.
authors: Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
authors: Tiehan
authors: T
authors: Zhao
authors: Ying
authors: Y
authors: Zeng
authors: Allison R
authors: AR
authors: Kermode
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics*; D017796:Caulimovirus / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D056726:Genetic Association Studies; D018525:Germination / Q000235:genetics / Q000502:physiology; D009154:Mutation / Q000235:genetics; D057445:Plant Dormancy / Q000235:genetics*; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000254:growth & development; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; C418905:FT protein, Arabidopsis; D010937:Plant Growth Regulators; C036483:T-DNA