title: Cellfood™ improves respiratory metabolism of endothelial cells and inhibits hypoxia-induced reactive oxygen species (ros) generation.
abstract: Endothelial mitochondria, the major site of ATP generation, modulate the intracellular dynamics of reactive oxygen species (ROS), which, in turn, control endothelial function. Adequate oxygen (O(2)) supply is required by endothelial cells (EC). Both hypoxia and hyperoxia may favor the overproduction of ROS leading to oxidative stress, mitochondrial damage and endothelial dysfunction. We investigated the capability and mechanisms of Cellfood™ (CF), an antioxidant compound, to modulate O(2) availability and mitochondrial respiratory metabolism and to regulate ROS generated by hypoxia in EC in vitro. Human umbilical vein endothelial cells (HUVEC) and ECV-304 were evaluated for the O(2) consumption using a Clark's electrode. The O(2) consumption rate rose, during the first minutes after CF addition and was associated with increase in mitochondrial oxidative capacity and good cell viability. Similar behaviours were observed when EC were exposed to CF for up to 8 days. The O(2) consumption increased and was accompanied by both intracellular rise of ATP and maintainment of LDH concentration. Hypoxia-induced ROS generation was significantly inhibited by CF, through the up-regulated expression of MnSOD, an anti-oxidant responsible for mitochondrial function preservation. The EC hypoxic response is mediated by the hypoxia master regulator HIF-1alpha whose activation was attenuated by CF, in concomitance with MnSOD up-regulation. Our results suggest a role for CF in improoving respiratory metabolism and in activating anti-oxidant mechanisms in EC, thus preserving endothelial function.
authors: Myeloma Unit, San Raffaele Scientific Institute, Milan, Italy.
authors: E
authors: E
authors: Ferrero
authors: A
authors: A
authors: Fulgenzi
authors: D
authors: D
authors: Belloni
authors: C
authors: C
authors: Foglieni
authors: M E
authors: ME
authors: Ferrero
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000596:Amino Acids / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D015687:Cell Hypoxia; D019069:Cell Respiration; D002470:Cell Survival; D042783:Endothelial Cells; D004798:Enzymes / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008903:Minerals / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013431:Sulfates / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D000975:Antioxidants; C532683:Cellfood; D004798:Enzymes; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008903:Minerals; D017382:Reactive Oxygen Species; D013431:Sulfates; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase