title: A novel canine model of portal vein stenosis plus thioacetamide administration-induced cirrhotic portal hypertension with hypersplenism.
abstract: Current large animal models that could closely resemble the typical features of cirrhotic portal hypertension in human have not been well established. Thus, we aimed to develop and describe a reliable and reproducible canine cirrhosis model of portal hypertension. A total of 30 mongrel dogs were randomly divided into four groups: 1 (control; n = 5), 2 (portal vein stenosis [PVS]; n = 5], 3 (thioacetamide [TAA]; n = 5), and 4 (PVS plus TAA; n = 15). After 4-months modeling period, liver and spleen CT perfusion, abdominal CT scans, portal hemodynamics, gastroscopy, hepatic function, blood routine, the bone marrow, liver, and spleen histology were studied. The animals in group 2 (PVS) developed extrahepatic portosystemic collateral circulation, particularly esophageal varices, without hepatic cirrhosis and portal hypertension. Animals from group 3 (TAA) presented mild cirrhosis and portal hypertension without significant symptoms of esophageal varices and hypersplenism. In contrast, animals from group 4 (PVS + TAA) showed well-developed micronodular and macronodular cirrhosis, associated with significant portal hypertension and hypersplenism. The combination of PVS and TAA represents a novel, reliable, and reproducible canine cirrhosis model of portal hypertension, which is associated with the typical characteristics of portal hypertension, including hypersplenism.
authors: Departments of General Surgery, Ningde Municipal Hospital, Ningde City, Fujian, China.
authors: Dexin
authors: D
authors: Lin
authors: Xianbin
authors: X
authors: Wu
authors: Xiaoke
authors: X
authors: Ji
authors: Qiyu
authors: Q
authors: Zhang
authors: YuanWei
authors: Y
authors: Lin
authors: WeiJian
authors: W
authors: Chen
authors: Wangxun
authors: W
authors: Jin
authors: Liming
authors: L
authors: Deng
authors: Yunzhi
authors: Y
authors: Chen
authors: Bicheng
authors: B
authors: Chen
authors: Jianmin
authors: J
authors: Li
mesh_terms: D000818:Animals; D003251:Constriction, Pathologic / Q000150:complications / Q000473:pathology*; D004195:Disease Models, Animal; D004285:Dogs; D005355:Fibrosis / Q000150:complications / Q000473:pathology; D005773:Gastroscopy; D006439:Hemodynamics; D006971:Hypersplenism / Q000150:complications* / Q000473:pathology*; D006975:Hypertension, Portal / Q000139:chemically induced* / Q000150:complications*; D008297:Male; D011169:Portal Vein; D011897:Random Allocation; D013853:Thioacetamide; D014057:Tomography, X-Ray Computed
chemical_list: D013853:Thioacetamide