title: Real time PCR for fast detection of the angiotensinogen polymorphisms.
abstract: OBJECTIVE
  to develop a rapid and reliable real-time PCR to detect polymorphisms of angiotensinogen (AGT), to compare the two methods of MS-PCR (Mutagenically Separated PCR) and real-time PCR to determine three polymorphisms of the angiotensinogen gene M235T, the A (-6) G and A (-20) C.
  
  
  METHODS
  the method of real-time PCR was developed on the PLC Roche LightCycler1 with SYBR Green I. We used two sense primers and a primer nonsense. Detection of polymorphisms of angiotensinogen gene was performed by comparing the melting curves.
  
  
  RESULTS
  the DNA samples were analyzed by two methods: real-time PCR and MS-PCR. In our study, no differences were found between the two techniques.
  
  
  CONCLUSIONS
  The real-time PCR is a rapid and reliable method for detecting gene polymorphisms on the AGT M235T, the A (-6) G and A (-20) C.
  
  
  CONCLUSIONS
  this method of real-time PCR is a reliable genetic test, which is fast and cheap and can be used in practice to study particular polymorphisms of AGT gene associated with cardiovascular disease.
authors: Department of clinical biochemistry, CHU-Farhat Hached, Sousse, Tunisia. azzadandana@yahoo.fr
authors: Azza
authors: A
authors: Dandana
authors: Imen
authors: I
authors: Gammoudi
authors: Salima
authors: S
authors: Ferchichi
authors: Hinda
authors: H
authors: Chahed
authors: Giampiero
authors: G
authors: Bricca
authors: Halima Ben
authors: HB
authors: Limem
authors: Faouzi
authors: F
authors: Maatouk
authors: Abdelhedi
authors: A
authors: Miled
mesh_terms: D000328:Adult; D000368:Aged; D000808:Angiotensinogen / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000201:enzymology* / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D000808:Angiotensinogen