title: Western blotting analysis as a tool to study receptor tyrosine kinases.
abstract: Receptor tyrosine kinases (RTKs) are involved in critical aspects of cell physiology ranging from cell -survival, proliferation, growth, migration, and differentiation. A tight control of the extent and duration of signals elicited by activated RTKs is crucial for preventing over-stimulation, which can ultimately lead to unrestrained proliferative ability and neoplastic growth. Ligand-induced downregulation of RTKs has emerged as a key negative regulatory mechanism that can accomplish signaling attenuation, by removing activated receptors from the cell surface and committing them to degradation. The ability of RTKs to escape from ligand-induced downregulation has been reported as a recurrent mechanism of oncogenic deregulation in cancer.Western blotting procedures have been extensively proven as straightforward assays to evaluate -protein expression levels and have been widely applied to study RTKs downregulation.
authors: School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Ireland. serena.germano@gmail.com
authors: Serena
authors: S
authors: Germano
authors: Lorraine
authors: L
authors: O'Driscoll
mesh_terms: D015153:Blotting, Western; D002277:Carcinoma / Q000201:enzymology; D045744:Cell Line, Tumor; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D010190:Pancreatic Neoplasms / Q000201:enzymology; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis*; D015398:Signal Transduction
chemical_list: D020794:Receptor Protein-Tyrosine Kinases