title: Three-dimensional network polyamidoamine dendrimer-Au nanocomposite for the construction of a mediator-free horseradish peroxidase biosensor.
abstract: A three-dimensional network PAMAM-Au nanocomposite (3D-PAMAM-Au NC) was prepared by using the first generation polyamidoamine dendrimer (G1 PAMAM) as the dispersant agent. The resultant 3D-PAMAM-Au NC was successfully used as an immobilization matrix for the construction of a reagentless mediator-free horseradish peroxidase (HRP)-based H(2)O(2) biosensor on a multi-walled carbon nanotubes (MWCNTs) modified glassy carbon electrode. With the advantages of the three-dimensional network, the organic-inorganic hybrid materials dramatically facilitate the direct electron transfer of HRP, and good bioelectrocatalytic activity towards H(2)O(2) was demonstrated. Under optimum conditions, the current response of the enzyme modified electrode at -0.30 V was detected. The current response is linearly correlated to H(2)O(2) concentration within the range of 18.00 μM to 20.80 mM with a correlation coefficient of 0.9992 and a sensitivity of 377.78 μA mM(-1) cm(-2). The detection limit was down to 6.72 μM (S/N = 3). Furthermore, the biosensor exhibits some other excellent characteristics, such as high selectivity, short response time, and long-term stability. The 3D-PAMAM-Au NC has proved to be a promising biosensing platform for the construction of mediator-free biosensors, and may find wide potential applications in biosensors, biocatalysis, bioelectronics and biofuel cells.
authors: Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, P R China.
authors: Jiao
authors: J
authors: Luo
authors: Mingnan
authors: M
authors: Dong
authors: Fanbo
authors: F
authors: Lin
authors: Meiling
authors: M
authors: Liu
authors: Hao
authors: H
authors: Tang
authors: Haitao
authors: H
authors: Li
authors: Youyu
authors: Y
authors: Zhang
authors: Shouzhuo
authors: S
authors: Yao
mesh_terms: D015374:Biosensing Techniques; D050091:Dendrimers; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized; D006046:Gold; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis*; D053768:Metal Nanoparticles; D053761:Nanocomposites; D037742:Nanotubes, Carbon; D012680:Sensitivity and Specificity
chemical_list: D050091:Dendrimers; D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; C104700:PAMAM Starburst; D006046:Gold; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase