title: [Effect of phospholipomannan of Candida albicans on the production of interleukin 6 and interleukin 8 in monocytes].
abstract: OBJECTIVE
  To investigate whether Candida albicans-native phospholipomannan (PLM) induce an inflammation response through Toll-like receptor(TLRé2 in human acute monocytic leukemia cell line (THP-1) cells.
  
  
  METHODS
  Human THP-1 monocytes were challenged with PLM in vitro. The mRNA expressions of TLR2, TLR4, proinflammatory cytokine [interleukin(IL)-6], and chemokine (IL-8) were assayed by real time reverse transcription polymerase chain reaction. The secretions of IL-6 and IL-8 were measured by enzyme-linked immunosorbent assay. The expression of TLR2 was analyzed with Western blot.
  
  
  RESULTS
  PLM increased the mRNA expressions and secretions of proinflammatory cytokines (IL-6) and chemokines (IL-8) in THP-1 cells (all P=0.0000). PLM up-regulated the mRNA and protein levels of TLR2 (P=0.0000), whereas the mRNA level of TLR4 was not altered. PLM hydrolyzed with β-D-mannoside manno hydrolase failed to induce gene and protein expressions of TLR2, IL-6, and IL-8. Anti-TLRS-neutralizing antibody blocked the PLM-induced secretions of IL-6 and IL-8 in THP-1 cells (P = 0.0003, P = 0.0010).
  
  
  CONCLUSIONS
  Canidada albicans-native PLM may contribute to the inflammatory responses during Candida infection in a TLR2-dependent manner.
authors: Jiangsu Province Blood Center, Nanjing, China.
authors: Qing
authors: Q
authors: Chen
authors: Min
authors: M
authors: Li
authors: Rong-cai
authors: RC
authors: Tang
authors: Wei-da
authors: WD
authors: Liu
authors: Wu-qing
authors: WQ
authors: Zhou
authors: Yong-nian
authors: YN
authors: Shen
authors: Gui-xia
authors: GX
authors: Lv
mesh_terms: D002176:Candida albicans / Q000737:chemistry*; D002478:Cells, Cultured; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; D006017:Glycolipids; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; C495342:TLR2 protein, human; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C400188:phospholipomannan