title: Prior laparotomy or corticosterone potentiates lipopolysaccharide-induced fever and sickness behaviors.
abstract: Stimulating sensitized immune cells with a subsequent immune challenge results in potentiated pro-inflammatory responses translating into exacerbated sickness responses (i.e. fever, pain and lethargy). Both corticosterone (CORT) and laparotomy cause sensitization, leading to enhanced sickness-induced neuroinflammation or pain (respectively). However, it is unknown whether this sensitization affects all sickness behaviors and immune cell responses equally. We show that prior CORT and prior laparotomy potentiated LPS-induced fever but not lethargy. Prior CORT, like prior laparotomy, was able to potentiate sickness-induced pain. Release of nitric oxide (NO) from peritoneal macrophages stimulated ex vivo demonstrates that laparotomy, but not CORT sensitizes these cells.
authors: Wadsworth Center, Albany, NY 12201-0509, USA.
authors: Leah E
authors: LE
authors: Hains
authors: Lisa C
authors: LC
authors: Loram
authors: Frederick R
authors: FR
authors: Taylor
authors: Keith A
authors: KA
authors: Strand
authors: Julie L
authors: JL
authors: Wieseler
authors: Ruth M
authors: RM
authors: Barrientos
authors: Jennifer J
authors: JJ
authors: Young
authors: Matthew G
authors: MG
authors: Frank
authors: Julia
authors: J
authors: Sobesky
authors: Thomas J
authors: TJ
authors: Martin
authors: James C
authors: JC
authors: Eisenach
authors: Steven F
authors: SF
authors: Maier
authors: John D
authors: JD
authors: Johnson
authors: Monika
authors: M
authors: Fleshner
authors: Linda R
authors: LR
authors: Watkins
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002478:Cells, Cultured; D003345:Corticosterone / Q000008:administration & dosage* / Q000633:toxicity*; D004357:Drug Synergism; D005334:Fever / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D016905:Gram-Negative Bacterial Infections / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D054884:Host-Pathogen Interactions / Q000276:immunology; D007114:Immunization; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D007813:Laparotomy / Q000009:adverse effects*; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000633:toxicity*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D010146:Pain / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008070:Lipopolysaccharides; D003345:Corticosterone