title: Biotransformation of 4-chloro-2-nitrophenol into 5-chloro-2-methylbenzoxazole by a marine Bacillus sp. strain MW-1.
abstract: Decolourization, detoxification and biotransformation of 4-chloro-2-nitrophenol (4C2NP) by Bacillus sp. strain MW-1 were studied. This strain decolorized 4C2NP only in the presence of an additional carbon source. On the basis of thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS), 4-chloro-2-aminophenol, 4-chloro-2-acetaminophenol and 5-chloro-2-methylbenzoxazole were identified as metabolites. Resting cells depleted 4C2NP with stoichiometric formation of 5-chloro-2-methyl benzoxazole. This is the first report of the formation of 5-chloro-2-methylbenzoxazole from 4C2NP by any bacterial strain.
authors: Environmental Biotechnology, Institute of Microbial Technology (CSIR), Sector-39A, Chandigarh, 160036, India. arora484@gmail.com
authors: Pankaj Kumar
authors: PK
authors: Arora
authors: Rakesh Kumar
authors: RK
authors: Jain
mesh_terms: D000641:Ammonia / Q000032:analysis; D001407:Bacillus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism*; D001583:Benzoxazoles / Q000378:metabolism*; D001711:Biotransformation; D002712:Chlorides / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003116:Color; D057075:Enzyme Assays; D013058:Mass Spectrometry; D009596:Nitrophenols / Q000737:chemistry / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D052160:Benzothiazoles; D001583:Benzoxazoles; D002712:Chlorides; D009596:Nitrophenols; D013438:Sulfhydryl Compounds; C068311:4-chloro-2-nitrophenol; D000641:Ammonia; C539674:5-chloro-2-mercaptobenzothiazole