title: Novel synthesis of steryl esters from phytosterols and amino Acid.
abstract: The feasibility of esterification of phytosterol with the amino acid l-glutamic acid was established. The influence of various organic solvents was investigated, and n-butanol was selected as an ideal solvent for phytosteryl esters synthesis with l-glutamic acid. The reaction conditions were further optimized by orthogonal experiments, and a 92.3% degree of esterification was obtained when optimum conditions were used. FT-IR spectral, GC-MS, and NMR analyses were adopted to determine the steryl esters of l-glutamic acid. The FT-IR spectrum indicated the presence of ester bonds in the phytosteryl esters with l-glutamic acid, and on the basis of the detailed mass spectrography analysis, GC-MS and NMR offered an efficient and reliable way to confirm the steryl esters. This novel synthesis approach of phytosteryl esters with amino acid supplied a promising alternative to the substrate on esterification of phytosterols and thus can be readily applied to further studies of functional food ingredients of phytosteryl esters.
authors: School of Biotechnology and Food Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
authors: Min
authors: M
authors: Pang
authors: Shaotong
authors: S
authors: Jiang
authors: Lili
authors: L
authors: Cao
authors: Lijun
authors: L
authors: Pan
mesh_terms: D020001:1-Butanol / Q000737:chemistry*; D004951:Esterification; D004952:Esters / Q000138:chemical synthesis*; D008401:Gas Chromatography-Mass Spectrometry; D018698:Glutamic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010840:Phytosterols / Q000737:chemistry*; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004952:Esters; D010840:Phytosterols; D012997:Solvents; D018698:Glutamic Acid; D020001:1-Butanol