title: An albumin-associated PLA2-like activity inactivates surfactant phosphatidylcholine secreted from fetal type II pneumocytes.
abstract: Type II pneumocytes are responsible for the synthesis and secretion of pulmonary surfactant, which reduces surface tension in lung alveoli, thus decreasing their tendency to collapse during expiration. For this effect to be sustained, the integrity of the surface-active components of surfactant must be maintained. This study has shown that, when cultured type II pneumocytes are exposed to lipoprotein-free serum (LFS), the level of lyso-phosphatidylcholine (lyso-PC) in the secreted surfactant phospholipids is markedly elevated with a concomitant decline in the level of phosphatidylcholine (PC). This effect is the result of hydrolysis of surfactant PC by a phospholipase A(2) (PLA(2))-like activity present within serum. Anion-exchange chromatography, gel filtration chromatography and preparative electrophoresis of human LFS have shown that this PLA(2)-like activity coelutes with albumin and is biochemically distinct from the secretory form of PLA(2). Furthermore, specific inhibitors of PLA(2) such as p-bromophenacyl bromide, aristolochic acid, and palmitoyl trifluoromethyl ketone do not inhibit this activity of serum. Commercially purified human serum albumin fraction V and recombinant human serum albumin (rHSA) are almost as effective as LFS in enhancing the level of lyso-PC in the media. The latter finding implies that rHSA directly generates lyso-PC from secreted PC and suggests that this PLA(2)-like activity may be an intrinsic attribute of albumin.
authors: School of Biological Sciences and Biotechnology, Murdoch University, Western Australia, Australia.
authors: Jolanta E
authors: JE
authors: Damas
authors: Max H
authors: MH
authors: Cake
mesh_terms: D056809:Alveolar Epithelial Cells / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004533:Egtazic Acid / Q000737:chemistry; D005333:Fetus / Q000166:cytology; D006801:Humans; D010713:Phosphatidylcholines / Q000378:metabolism*; D064801:Phospholipase A2 Inhibitors; D054467:Phospholipases A2 / Q000097:blood* / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D044967:Serum; D012709:Serum Albumin / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines; D064801:Phospholipase A2 Inhibitors; D010743:Phospholipids; D011994:Recombinant Proteins; D012709:Serum Albumin; D004533:Egtazic Acid; D054467:Phospholipases A2