title: Cationic solid lipid nanoparticles (cSLN): structure, stability and DNA binding capacity correlation studies.
abstract: Cationic solid lipid nanoparticles (cSLN) are promising lipid nanocarriers for intracellular gene delivery based on well-known and widely accepted materials. cSLN containing single-chained cationic lipid cetyltrimethylammonium bromide were produced by high pressure homogenization and characterized in terms of (a) particle size distribution by photon correlation spectroscopy (PCS) and laser diffractometry (LD), (b) thermal behaviour using differential scanning calorimetry (DSC) and (c) the presence of various polymorphic phases was confirmed by X-ray diffraction (WAXD). SLN composed of Imwitor 900P (IMW) showed different pDNA stability and binding capacity in comparison to those of Compritol 888 ATO (COM). IMW-SLN, having z-ave=138-157 nm and d(0.5)=0.15-0.158 μm could maintain this size for 14 days at room temperature. COM-SLN had z-ave=334 nm and d(0.5)=0.42 μm on the day of production and could maintain similar size during 90 days. IMW-SLN revealed improved pDNA binding capacity. We attempted to explain these differences by different interactions between the solid lipid and the tested cationic lipid.
authors: Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD), Vila-Real, Portugal.
authors: S
authors: S
authors: Doktorovova
authors: R
authors: R
authors: Shegokar
authors: E
authors: E
authors: Rakovsky
authors: E
authors: E
authors: Gonzalez-Mira
authors: C M
authors: CM
authors: Lopes
authors: A M
authors: AM
authors: Silva
authors: P
authors: P
authors: Martins-Lopes
authors: R H
authors: RH
authors: Muller
authors: E B
authors: EB
authors: Souto
mesh_terms: D002152:Calorimetry, Differential Scanning; D002412:Cations / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers; D004355:Drug Stability; D005989:Glycerides / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D002412:Cations; D002593:Cetrimonium Compounds; D004337:Drug Carriers; D005989:Glycerides; D013501:Surface-Active Agents; D020442:Poloxamer; D004247:DNA; D000077286:Cetrimonium