title: Accelerating microfluidic immunoassays on filter membranes by applying vacuum.
abstract: This paper describes a vacuum-accelerated microfluidic immunoassay (we abbreviate it as VAMI) by sandwiching a filter membrane between a two-layer chip. A direct assay of IgG demonstrated that VAMI could simultaneously achieve higher sensitivity and require less time compared with conventional microfluidic immunoassays. We further applied VAMI to carry out a 3-step competitive assay (including antigen immobilization, competitive reaction and 2(nd) antibody reaction) for detecting the illegal food additive Sudan Red. A total assay time of 15 min with a limit of detection (LOD) of 1 ng ml(-1) is achieved.
authors: CAS Key lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, 11 Beiyitiao, ZhongGuanCun, Beijing, 100190, China.
authors: Yingyi
authors: Y
authors: Liu
authors: Jie
authors: J
authors: Yu
authors: Meihong
authors: M
authors: Du
authors: Wenjun
authors: W
authors: Wang
authors: Wei
authors: W
authors: Zhang
authors: Zhuo
authors: Z
authors: Wang
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D014618:Vacuum