title: Gene Composer in a structural genomics environment.
abstract: The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino-acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high-throughput protein structure determination, a database software package, Gene Composer, has been developed which facilitates the information-rich design of protein constructs and their underlying gene sequences. With its modular workflow design and numerous graphical user interfaces, Gene Composer enables researchers to perform all common bioinformatics steps used in modern structure-guided protein engineering and synthetic gene engineering. An example of the structure determination of H1N1 RNA-dependent RNA polymerase PB2 subunit is given.
authors: Emerald BioStructures, 7869 NE Day Road West, Bainbridge Island, WA 98110, USA. dlorimer@embios.com
authors: Don
authors: D
authors: Lorimer
authors: Amy
authors: A
authors: Raymond
authors: Mark
authors: M
authors: Mixon
authors: Alex
authors: A
authors: Burgin
authors: Bart
authors: B
authors: Staker
authors: Lance
authors: L
authors: Stewart
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D023281:Genomics; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D012984:Software
chemical_list: D011506:Proteins