title: Monoolein lipid phases as incorporation and enrichment materials for membrane protein crystallization.
abstract: The crystallization of membrane proteins in amphiphile-rich materials such as lipidic cubic phases is an established methodology in many structural biology laboratories. The standard procedure employed with this methodology requires the generation of a highly viscous lipidic material by mixing lipid, for instance monoolein, with a solution of the detergent solubilized membrane protein. This preparation is often carried out with specialized mixing tools that allow handling of the highly viscous materials while minimizing dead volume to save precious membrane protein sample. The processes that occur during the initial mixing of the lipid with the membrane protein are not well understood. Here we show that the formation of the lipidic phases and the incorporation of the membrane protein into such materials can be separated experimentally. Specifically, we have investigated the effect of different initial monoolein-based lipid phase states on the crystallization behavior of the colored photosynthetic reaction center from Rhodobacter sphaeroides. We find that the detergent solubilized photosynthetic reaction center spontaneously inserts into and concentrates in the lipid matrix without any mixing, and that the initial lipid material phase state is irrelevant for productive crystallization. A substantial in-situ enrichment of the membrane protein to concentration levels that are otherwise unobtainable occurs in a thin layer on the surface of the lipidic material. These results have important practical applications and hence we suggest a simplified protocol for membrane protein crystallization within amphiphile rich materials, eliminating any specialized mixing tools to prepare crystallization experiments within lipidic cubic phases. Furthermore, by virtue of sampling a membrane protein concentration gradient within a single crystallization experiment, this crystallization technique is more robust and increases the efficiency of identifying productive crystallization parameters. Finally, we provide a model that explains the incorporation of the membrane protein from solution into the lipid phase via a portal lamellar phase.
authors: Emerald BioStructures, Bainbridge Island, Washington, United States of America.
authors: Ellen
authors: E
authors: Wallace
authors: David
authors: D
authors: Dranow
authors: Philip D
authors: PD
authors: Laible
authors: Jeff
authors: J
authors: Christensen
authors: Peter
authors: P
authors: Nollert
mesh_terms: D003460:Crystallization / Q000379:methods*; D005989:Glycerides / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008954:Models, Biological; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry; D012242:Rhodobacter sphaeroides / Q000737:chemistry*; D013997:Time Factors
chemical_list: D005989:Glycerides; D008055:Lipids; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; C005953:monoolein