title: DNA unzipping and force measurements with a dual optical trap.
abstract: In order to open the DNA double helix mechanically, a molecular construction is prepared which allows specific attachment of the two complementary strands of an individual molecule to two different μm-sized beads. The beads are separately captured by a dual optical trap, thus holding the DNA construction in solution. The opening of a molecule is obtained by increasing the distance between the traps, one trap being slowly moved while the other is held fixed. Force is measured to sub-piconewton precision by back focal plane interferometry of the bead in the fixed trap. The experiment allows us to measure base sequence-dependent force signal. In this chapter, important technical aspects of this type of single-molecule force measurements are considered.
authors: Laboratoire de Nanobiophysique, UMR Gulliver CNRS - ESPCI, Paris, France.
authors: Ismaïl
authors: I
authors: Cissé
authors: Pierre
authors: P
authors: Mangeol
authors: Ulrich
authors: U
authors: Bockelmann
mesh_terms: D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D052898:Optical Tweezers
chemical_list: D004247:DNA