title: Inulin supplementation in rat model of pouchitis.
abstract: Available data indicates potential effectiveness of prebiotic therapy in alleviating inflammation and prolonging the remission in inflammatory bowel disease. Documented successes of such therapies were the basis for this study. So far, there is no data related to the effectiveness of inulin application in symptomatic or severe pouchitis in humans or in animal model. The aim of the study was to determine the effect of inulin supplementation on the expression of intestinal inflammation and feeding efficiency in rats with induced pouchitis. Twenty-four Wistar rats were operated. After induction of pouchitis animals were randomly divided into control and supplementation groups receiving, respectively, semi-synthetic diet with or without inulin (in a lower (LD) or higher (HD) dose: 2.5 % or 5 % of total dietary content of mass) for a period of 6 weeks. Selected nutritional parameters were assessed throughout the study. Histopathological and immunohistochemical analysis of pouch mucosa specimens was also performed. The energy intake, weight gain, feeding efficiency, quality of stools were comparable in all studied groups. The intensity of inflammation (Moskovitz scale) and adaptive changes (Laumonier scale) did not differ between compared groups. The tissue expression of pro- and anti-inflammatory interleukins (IL-1α, IL-6, IL-10 and IL-12)  was not different either. Inulin supplementation does not improve the quality of stools or the expression of intestinal inflammation in rats with induced pouchitis. It has no impact on the intensity of pouch adaptation or on feeding efficiency.
authors: Department of Human Nutrition and Hygiene, Poznań University of Life Sciences, Poznań, Poland.
authors: Sławomira
authors: S
authors: Drzymała-Czyż
authors: Tomasz
authors: T
authors: Banasiewicz
authors: Małgorzata
authors: M
authors: Tubacka
authors: Przemysław
authors: P
authors: Majewski
authors: Maciej
authors: M
authors: Biczysko
authors: Tomasz
authors: T
authors: Kościński
authors: Michał
authors: M
authors: Drews
authors: Jarosław
authors: J
authors: Walkowiak
mesh_terms: D000818:Animals; D019587:Dietary Supplements; D004195:Disease Models, Animal; D002149:Energy Intake; D005243:Feces; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D053582:Interleukin-1alpha / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007444:Inulin / Q000494:pharmacology*; D019449:Pouchitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015430:Weight Gain
chemical_list: D053582:Interleukin-1alpha; D015850:Interleukin-6; D016753:Interleukin-10; D018664:Interleukin-12; D007444:Inulin