title: Fluorescent europium-modified polymer nanoparticles for rapid and sensitive anthrax sensors.
abstract: Novel fluorescent polyacrylonitrile nanoparticles were synthesized by microemulsion polymerization and Schiff base modification. By further modification with europium, the polyacrylonitrile nanoparticles could be used as a highly sensitive and rapid sensor for Bacillus anthracis spore detection in aqueous solution. The europium-modified polyacrylonitrile nanoparticles were readily combined with dipicolinic acid as a unique biomarker of B. anthracis, leading to high fluorescence emission. These nanoparticles enabled ratiometric detection without instrument-specific calibration due to the internal fluorescence reference. Additionally, the europium-modified polyacrylonitrile nanoparticle sensors exhibited a remarkable limit of detection (10pM) for dipicolinic acid and outstanding selectivity (160×) over aromatic ligands in aqueous solution. The ultrafine nanoparticle sensor showed a high capability for detecting anthrax due to the increased surface area-to-volume ratio and enhanced dispersibility.
authors: World Class University (WCU) Program of Chemical Convergence for Energy & Environment (C(2)E(2)), School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: Wan-Kyu
authors: WK
authors: Oh
authors: Yoon Seon
authors: YS
authors: Jeong
authors: Jooyoung
authors: J
authors: Song
authors: Jyongsik
authors: J
authors: Jang
mesh_terms: D000180:Acrylic Resins; D001408:Bacillus anthracis / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D005063:Europium; D005456:Fluorescent Dyes; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010848:Picolinic Acids / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013171:Spores, Bacterial / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000180:Acrylic Resins; D005456:Fluorescent Dyes; D010848:Picolinic Acids; C010504:polyacrylonitrile; D005063:Europium; C004860:dipicolinic acid