title: Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells.
abstract: BACKGROUND
  Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation.
  
  
  RESULTS
  Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics.
  
  
  CONCLUSIONS
  This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.
authors: Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
authors: Sarah
authors: S
authors: Paule
authors: Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
authors: Katie
authors: K
authors: Meehan
authors: Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
authors: Adam
authors: A
authors: Rainczuk
authors: Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
authors: Andrew N
authors: AN
authors: Stephens
authors: Prince Henrys Institute of Medical Research, Clayton 3168 Australia.
authors: Guiying
authors: G
authors: Nie