title: Recent advances in dynamic intravital multi-photon microscopy.
abstract: Standard multiphoton laser scanning microscopy (MPLSM) has revolutionized our view of physiologic and pathologic processes in living organisms by enlightening different aspects of cellular choreography in immune responses, that is, cellular motility and co-localization. To understand cellular communication on a molecular level, novel transgenic reporter mice have been generated. In parallel, MPLSM systems have been developed, which make it possible for this technique to be more widely used to address crucial immunological questions. Here, we review the latest progress concerning transgenic mouse technology and multiphoton imaging capacities and discuss further developments which will enable us to visualize all around monitoring and quantification of cellular function at a molecular level directly in vivo.
authors: Biophysical Analytics, Deutsches Rheumaforschungszentrum (a Leibniz Institute), Charitéplatz 1, 10117 Berlin, Germany.
authors: Raluca A
authors: RA
authors: Niesner
authors: Anja E
authors: AE
authors: Hauser
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D007834:Lasers; D008027:Light; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation / Q000379:methods*; D055668:Photochemical Processes / Q000528:radiation effects; D017785:Photons
chemical_list: D005456:Fluorescent Dyes