title: Establishment of gerbil-mouse heterohybridoma secreting immunoglobulin of Mongolian gerbil (Meriones unguiculatus) establishment of gerbil-mouse heterohybridoma.
abstract: Hybridomas, generated by fusing myeloma cells with B lymphocytes, secrete monoclonal antibodies (mAbs) specific for an antigen. To establish hybridomas that secrete Mongolian gerbil (Meriones unguiculatus) mAbs, we immunized gerbils with keyhole limpet hemocyanin (KLH) and fused splenocytes from them with a non-secreting mouse myeloma cell line (P3-X63-Ag8.653). We obtained hybridomas that secreted immunoglobulin (Ig) against KLH. These hybridomas had more chromosomes than either parent and retained several gerbil chromosomes. Therefore, these cells were heterohybridomas with both gerbil and mouse chromosomes. They expressed gerbil Ig heavy-chain constant (IGHC) region mRNA, but not mouse gamma (γ) 1 IGHC. SDS-PAGE and Western blot analyses indicated that the cells secreted whole Ig molecules of gerbil origin. Moreover, the cells continued secreting Ig for several months.
authors: Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa 252-0880, Japan.
authors: Takao
authors: T
authors: Ukaji
authors: Masahiro A
authors: MA
authors: Iwasa
authors: Osamu
authors: O
authors: Kai
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002459:Cell Fusion; D045744:Cell Line, Tumor; D033481:Chromosomes, Mammalian / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005849:Gerbillinae / Q000235:genetics / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D007070:Immunoglobulin A / Q000235:genetics / Q000276:immunology; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice / Q000235:genetics / Q000276:immunology*; D008813:Mice, Inbred ICR; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D007136:Immunoglobulins; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin