title: Effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b in male rats.
abstract: This study investigated the effects of cadmium on bone microstructure and serum tartrate-resistant acid phosphatase 5b (Tracp 5b) in male rats. Sprague-Dawley male rats were divided into three groups that were given CdCl(2) by subcutaneous injection at doses of 0, 0.1 and 0.5 mg/kg body weight (bw) for 12 weeks, respectively. Before killing at the 12th week, microcomputed tomography scanning was performed on the proximal tibia, and urine samples were collected from all of the rats. All rats were then killed, and their blood was collected for biomarkers assay. Bone tissues were dissected for mineral density determinations and histology. The concentration of cadmium in the blood, urine and bone of rats treated with cadmium were significantly higher than in the control group. The bone mineral density, bone mineral concentrations and bone microstructure index of rats treated with cadmium at 0.5 mg/kg bw were clearly lower than in the control rats. Histological investigation also revealed damage to the bone microstructure caused by cadmium. Tracp 5b concentrations in rats treated with cadmium were dose dependently higher than the control. The concentration of cadmium in blood, urine and bone was significantly correlated with Tracp 5b and bone microstructure parameters. Cadmium was shown to induce bone microstructure damage, especially to trabecular bone. The elevated concentrations of serum Tracp 5b suggest that bone resorption mediated via osteoclasts is an important mechanism for the toxic effects of cadmium on bone.
authors: Institute of Radiation Medicine, Fudan University, Shanghai 200032, China.
authors: Xiao
authors: X
authors: Chen
authors: Guoying
authors: G
authors: Zhu
authors: Chunlin
authors: C
authors: Shao
authors: Taiyi
authors: T
authors: Jin
authors: Mingguang
authors: M
authors: Tan
authors: Shuzhu
authors: S
authors: Gu
authors: Yanyan
authors: Y
authors: Zhang
authors: Hanfang
authors: H
authors: Xiao
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D002104:Cadmium / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D055114:X-Ray Microtomography
chemical_list: D015415:Biomarkers; D007527:Isoenzymes; D002104:Cadmium; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase