title: [Detection of genes associated with drug resistance in Mycobacterium tuberculosis strains isolated in Chile].
abstract: BACKGROUND
  The incidence of acquired resistance to antituberculous drugs of Mycobacterium tuberculosis in Chile is approximately 23%.
  
  
  OBJECTIVE
  To analyze the mutations associated with drug resistance in drug resistant strains of Mycobacterium tuberculosis.
  
  
  METHODS
  In 28 drug resistant Mycobacterium tuberculosis strains isolated in Chile, genes leading to drug resistance were studied. DNA was amplified by polymerase chain reaction (PCR) and sequencing was carried out using the ABI PRISM big dye terminator cycle sequencing ready reaction kit.
  
  
  RESULTS
  In rifampicin-resistant strains, the mutations in rpoβ gene were in the codons S531W/L (56%), D516Y (16%) and D516V (16%). The predominant mutation in katG gene was in the codon S315L (73%) in isoniazid-resistant strains. The mutation S95T was found in the 71% of ciprofloxacin resistant strains. Only one ethambutol resistant strain had the M306I mutation. Three unreported mutations in katG were identified.
  
  
  CONCLUSIONS
  Drug resistance associated mutations of Mycobacterium tuberculosis isolated in Chile were similar to those reported abroad.
authors: Subdepartamento Genética Molecular, Instituto de Salud Pública de Chile, Chile.
authors: Pamela
authors: P
authors: Araya
authors: Maritza
authors: M
authors: Velasco
authors: Javier
authors: J
authors: Tognarelli
authors: Fabiola
authors: F
authors: Arias
authors: Tamara
authors: T
authors: Leiva
authors: Angélica
authors: A
authors: Sccapatticio
authors: Pablo
authors: P
authors: Alviz
authors: Jorge
authors: J
authors: Fernández
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D002677:Chile; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014157:Transcription Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000235:genetics / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C400682:VGLL1 protein, human