title: Endothelial nitric oxide synthase haplotypes are associated with preeclampsia in Maya mestizo women.
abstract: Preeclampsia is a specific disease of pregnancy and believed to have a genetic component. The aim of this study was to investigate if three polymorphisms in eNOS or their haplotypes are associated with preeclampsia in Maya mestizo women. A case-control study was performed where 127 preeclamptic patients and 263 controls were included. Genotyped and haplotypes for the -768T→C, intron 4 variants, Glu298Asp of eNOS were determined by PCR and real-time PCR allelic discrimination. Logistic regression analysis with adjustment for age and body mass index (BMI) was used to test for associations between genotype and preeclampsia under recessive, codominant and dominant models. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation <formula>r^{2}</formula>, and haplotype analysis was conducted. Women homozygous for the Asp298 allele showed an association of preeclampsia. In addition, analysis of the haplotype frequencies revealed that the -786C-4b-Asp298 haplotype was significantly more frequent in preeclamptic patients than in controls (0.143 vs. 0.041, respectively; OR =3.01; 95% CI = 1.74-5.23; P =2.9 × 10<formula>^{-4}</formula>).Despite the Asp298 genotype in a recessive model associated with the presence of preeclampsia in Maya mestizo women, we believe that in this population the -786C-4b-Asp298 haplotype is a better genetic marker.
authors: División de Investigación Biomédica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México.
authors: Lizbeth
authors: L
authors: Díaz-Olguín
authors: Ramón Mauricio
authors: RM
authors: Coral-Vázquez
authors: Thelma
authors: T
authors: Canto-Cetina
authors: Samuel
authors: S
authors: Canizales-Quinteros
authors: Belem
authors: B
authors: Ramírez Regalado
authors: Genny
authors: G
authors: Fernández
authors: Patricia
authors: P
authors: Canto
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006239:Haplotypes; D006801:Humans; D008800:Mexico; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D055815:Young Adult
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III