title: Fluorescence imaging of single Kinesin motors on immobilized microtubules.
abstract: Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.
authors: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors: Till
authors: T
authors: Korten
authors: Bert
authors: B
authors: Nitzsche
authors: Chris
authors: C
authors: Gell
authors: Felix
authors: F
authors: Ruhnow
authors: Cécile
authors: C
authors: Leduc
authors: Stefan
authors: S
authors: Diez
mesh_terms: D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D008962:Models, Theoretical; D045663:Quantum Dots
chemical_list: D016547:Kinesins