title: Value of (1-3)-β-d-glucan, Candida mannan and Candida DNA detection in the diagnosis of candidaemia.
abstract: This study determined the value of (1,3)-β-d-glucan (BDG), Candida mannan (MN) and Candida species-specific DNA as surrogates for diagnosis of candidaemia. Thirty-nine patients yielding Candida species in blood cultures were investigated for presence of BDG, MN and Candida species-specific DNA in serum samples. The Candida spp. bloodstream isolates included C. albicans (n = 16), C. tropicalis (n = 10), C. parapsilosis (n = 7), C. glabrata (n = 3) and C. dubliniensis (n = 3). Positivity of the three markers was as follows: Candida DNA for corresponding Candida species, 100%; BDG, 87%; MN, 59%. Despite varying sensitivities of these biomarkers, they provided a useful adjunct to the diagnosis of candidaemia.
authors: Department of Microbiology, Faculty of Medicine, Kuwait University.
authors: E
authors: E
authors: Mokaddas
authors: Z U
authors: ZU
authors: Khan
authors: S
authors: S
authors: Ahmad
authors: M R N
authors: MR
authors: Nampoory
authors: M
authors: M
authors: Burhamah
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002175:Candida / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D058387:Candidemia / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004271:DNA, Fungal / Q000097:blood* / Q000235:genetics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008351:Mannans / Q000097:blood*; D008875:Middle Aged; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D011509:Proteoglycans; D012680:Sensitivity and Specificity; D013045:Species Specificity; D055815:Young Adult; D047071:beta-Glucans / Q000097:blood*
chemical_list: D015415:Biomarkers; D004271:DNA, Fungal; D008351:Mannans; D011509:Proteoglycans; D047071:beta-Glucans; C010770:polysaccharide-K