title: Proteomic studies on protective effects of salvianolic acids, notoginsengnosides and combination of salvianolic acids and notoginsengnosides against cardiac ischemic-reperfusion injury.
abstract: BACKGROUND
  Salvia miltiorrhiza and Panax notoginseng are popularly used traditional Chinese medicine for cardiovascular disorders and they are often used in the form of combination. However, mechanisms of their cardioprotective effects were still not clear. In the present study, the protective effects of salvianolic acids (SA), notoginsengnosides (NG) and combination of SA and NG (CSN) against rat cardiac ischemia-reperfusion injury were checked and the protein expression profiles of heart tissues were examined to search their possible protein targets.
  
  
  METHODS
  The cardioprotective effects of SA, NG and CSN were checked in a rat model of ischemia-reperfusion (IR) by temporarily occluding coronary artery for 20 min followed by reperfusion. Rats were grouped into sham-operation group, IR group, IR+SA group, IR+NG group and IR+CSN group. The plasma creatine kinase (CK) activities were measured using commercial kit and the percentages of infarcted area in total ventricle tissue were calculated after nitroblue-tetrazolium (N-BT) staining of heart tissue slices. Two-dimensional protein electrophoresis (2-DE) was used to check the protein expression profiles of heart tissues. Then, proteins differentially expressed between IR group and sham-operation group were identified using matrix assisted laser desorption ionization-time of flight-mass spectrometry/mass spectrometry (MALDI-TOF MS/MS). The regulative effects of SA, NG and CSN on these IR-related proteins were analyzed.
  
  
  RESULTS
  Treatments including SA, NG and CSN all showed cardioprotective effects against ischemia-reperfusion injury and CSN exhibited to be the best. Eighteen proteins involved in IR injury were found. These proteins are involved in pathways including energy metabolism, lipid metabolism, muscle contraction, heat shock stress, cell survival and proliferation. The regulation of these proteins by SA, NG or CSN suggested possible protein targets in their cardioprotective effects.
  
  
  CONCLUSIONS
  SA and NG showed both similarity and difference in their protein targets involved in cardioprotective effects. The capability of CSN to regulate both protein targets of SA and NG might be the basis of CSN to show cardioprotective effects better than that of SA or NG.
authors: Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, PR China.
authors: Qing-Xi
authors: QX
authors: Yue
authors: Fu-Bo
authors: FB
authors: Xie
authors: Xiao-Yi
authors: XY
authors: Song
authors: Wan-Ying
authors: WY
authors: Wu
authors: Bao-Hong
authors: BH
authors: Jiang
authors: Shu-Hong
authors: SH
authors: Guan
authors: Min
authors: M
authors: Yang
authors: Xuan
authors: X
authors: Liu
authors: De-An
authors: DA
authors: Guo
mesh_terms: D000475:Alkenes / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D008297:Male; D008516:Medicine, Chinese Traditional; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D053723:Panax notoginseng / Q000737:chemistry; D010946:Plants, Medicinal; D059808:Polyphenols / Q000302:isolation & purification / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D000475:Alkenes; D004365:Drugs, Chinese Herbal; D059808:Polyphenols; D012503:Saponins; C568740:salvianolic acid; D003402:Creatine Kinase