title: Genetic diversity of carbapenem-hydrolysing β-lactamases in Acinetobacter baumannii from Romanian hospitals.
abstract: Thirteen carbapenem-resistant Acinetobacter baumannii isolates, collected in Romania during 2009-2010, were investigated to identify the mechanism(s) responsible for carbapenem resistance. Genotyping was performed by pulsed-field gel electrophoresis, multiplex PCR sequence typing and multilocus sequence typing. Eleven non-clonally related isolates harboured the bla(OXA-23) gene on their chromosome within a Tn2008 transposon structure. The two remaining isolates harboured a bla(OXA-58) gene that was either plasmid or chromosome borne. Two isolates co-expressed OXA-23 together with the extended-spectrum β-lactamase PER-1. This study constitutes the first report of OXA-58 and OXA-23-producing A. baumannii isolates in Romania.
authors: Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K. Bicêtre, France.
authors: R A
authors: RA
authors: Bonnin
authors: L
authors: L
authors: Poirel
authors: M
authors: M
authors: Licker
authors: P
authors: P
authors: Nordmann
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D012383:Romania / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D015780:Carbapenems; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C534889:beta-lactamase OXA-58, Acinetobacter baumannii