title: Two-dimensional and three-dimensional viability measurements of adult stem cells with optical coherence phase microscopy.
abstract: Cell viability assays are essential tools for cell biology. They assess healthy cells in a sample and enable the quantification of cellular responses to reagents of interest. Noninvasive and label-free assays are desirable in two-dimensional (2D) and three-dimensional (3D) cell culture to facilitate time-course viability studies. Cellular micromotion, emanating from cell to substrate distance variations, has been demonstrated as a marker of cell viability with electric cell-substrate impedance sensing (ECIS). In this study we investigated if optical coherence phase microscopy (OCPM) was able to report phase fluctuations of adult stem cells in 2D and 3D that could be associated with cellular micromotion. An OCPM has been developed around a Thorlabs engine (λo = 930 nm) and integrated in an inverted microscope with a custom scanning head. Human adipose derived stem cells (ADSCs, Invitrogen) were cultured in Mesenpro RS medium and seeded either on ECIS arrays, 2D cell culture dishes, or in 3D highly porous microplotted polymeric scaffolds. ADSC micromotion was confirmed by ECIS analysis. Live and fixed ADSCs were then investigated in 2D and 3D with OCPM. Significant differences were found in phase fluctuations between the different conditions. This study indicated that OCPM could potentially assess cell vitality in 2D and in 3D microstructures.
authors: University of Edinburgh, MRC Centre for Regenerative Medicine, Edinburgh, United Kingdom. pierre.bagnaninchi@ed.ac.uk
authors: Pierre O
authors: PO
authors: Bagnaninchi
authors: Christina
authors: C
authors: Holmes
authors: Nicola
authors: N
authors: Drummond
authors: Jamal
authors: J
authors: Daoud
authors: Maryam
authors: M
authors: Tabrizian
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D053687:Adult Stem Cells / Q000737:chemistry* / Q000166:cytology*; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D017097:Electric Impedance; D005583:Fourier Analysis; D006801:Humans; D008853:Microscopy / Q000379:methods*; D054457:Tissue Scaffolds; D041623:Tomography, Optical Coherence / Q000379:methods*