title: The effects of gold nanoparticles in wound healing with antioxidant epigallocatechin gallate and α-lipoic acid.
abstract: Topical applications of antioxidant agents in cutaneous wounds have attracted much attention. Gold nanoparticles (AuNPs), epigallocatechin gallate (EGCG), and α-lipoic acid (ALA) were shown to have antioxidative effects and could be helpful in wound healing. Their effects in Hs68 and HaCaT cell proliferation and in mouse cutaneous wound healing were studied. Both the mixture of EGCG + ALA (EA) and AuNPs + EGCG + ALA (AuEA) significantly increased Hs68 and HaCaT proliferation and migration. Topical AuEA application accelerated wound healing on mouse skin. Immunoblotting of wound tissue showed significant increase of vascular endothelial cell growth factor and angiopoietin-1 protein expression, but no change of angiopoietin-2 or CD31 after 7 days. After AuEA treatment, CD68 protein expression decreased and Cu/Zn superoxide dismutase increased significantly in the wound area. In conclusion, AuEA significantly accelerated mouse cutaneous wound healing through anti-inflammatory and antioxidation effects. This study may support future studies using other antioxidant agents in the treatment of cutaneous wounds.
  
  
  BACKGROUND
  In this study, topically applied gold nanoparticles with epigallocatechin gallate and alpha-lipoic acid were studied regarding their effects in wound healing in cell cultures. Significant acceleration was demonstrated in wound healing in a murine model.
authors: Division of Nephrology, Department of Internal Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.
authors: Jyh-Gang
authors: JG
authors: Leu
authors: Siang-An
authors: SA
authors: Chen
authors: Han-Min
authors: HM
authors: Chen
authors: Wen-Mein
authors: WM
authors: Wu
authors: Chi-Feng
authors: CF
authors: Hung
authors: Yeong-Der
authors: YD
authors: Yao
authors: Chi-Shun
authors: CS
authors: Tu
authors: Yao-Jen
authors: YJ
authors: Liang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006046:Gold / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D018919:Neovascularization, Physiologic; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000293:injuries; D008063:Thioctic Acid / Q000008:administration & dosage*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000975:Antioxidants; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D008063:Thioctic Acid; D006046:Gold; D002392:Catechin; C045651:epigallocatechin gallate; C046366:angiogenin; D012259:Ribonuclease, Pancreatic