title: Bacterial inactivation of wound infection in a human skin model by liquid-phase discharge plasma.
abstract: BACKGROUND
  We investigate disinfection of a reconstructed human skin model contaminated with biofilm-formative Staphylococcus aureus employing plasma discharge in liquid.
  
  
  RESULTS
  We observed statistically significant 3.83-log10 (p<0.001) and 1.59-log10 (p<0.05) decreases in colony forming units of adherent S. aureus bacteria and 24 h S. aureus biofilm culture with plasma treatment. Plasma treatment was associated with minimal changes in histological morphology and tissue viability determined by means of MTT assay. Spectral analysis of the plasma discharge indicated the presence of highly reactive atomic oxygen radicals (777 nm and 844 nm) and OH bands in the UV region. The contribution of these and other plasma-generated agents and physical conditions to the reduction in bacterial load are discussed.
  
  
  CONCLUSIONS
  These findings demonstrate the potential of liquid plasma treatment as a potential adjunct therapy for chronic wounds.
authors: Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado, United States of America.
authors: Paul Y
authors: PY
authors: Kim
authors: Yoon-Sun
authors: YS
authors: Kim
authors: Il Gyo
authors: IG
authors: Koo
authors: Jae Chul
authors: JC
authors: Jung
authors: Gon Jun
authors: GJ
authors: Kim
authors: Myeong Yeol
authors: MY
authors: Choi
authors: Zengqi
authors: Z
authors: Yu
authors: George J
authors: GJ
authors: Collins
mesh_terms: D018441:Biofilms / Q000187:drug effects; D004203:Disinfection / Q000379:methods*; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects*; D008954:Models, Biological; D055620:Optical Phenomena; D010949:Plasma / Q000737:chemistry / Q000378:metabolism*; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*; D013696:Temperature; D014022:Tissue Survival; D014946:Wound Infection / Q000382:microbiology* / Q000473:pathology