title: Axenic culture and identification of amastigotes from Sichuan human strain of Chinese Leishmania isolates.
abstract: This work describes a simple method to yield large amounts of the isolate MHOM/CN/90/SC10H2 amastigotes-like forms in axenic cultures using promastigotes as the starting population. The isolate MHOM/CN/90/SC10H2, used in this study, belongs to an undescribed species of Leishmania endemic to hill foci in China. The method describes induced extracellular amastigote transformation of this isolate. The rounded parasite obtained in axenic culture was morphologically similar, even at the ultrastructural level, to intracellular amastigotes. Moreover, the axenic amastigotes remained viable as verified by the stage-specific genes (gp46 and p4 genes) with RT-PCR. A 70-80 kDa protein was recognized by polyclonal antibody HRP-IgG only in axenic-derived amastigotes and not in promastigotes.
authors: Department of Parasitology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17 Section 3, Renmin South Road, Chengdu 610041, Sichuan, China.
authors: De-Ping
authors: DP
authors: Cao
authors: Da-Li
authors: DL
authors: Chen
authors: Jian-Ping
authors: JP
authors: Chen
authors: Lin
authors: L
authors: Liao
authors: Bin-Bin
authors: BB
authors: Yang
authors: Xiao-Su
authors: XS
authors: Hu
mesh_terms: D061245:Axenic Culture / Q000379:methods*; D002681:China; D006801:Humans; D015151:Immunoblotting; D007891:Leishmania / Q000145:classification / Q000166:cytology* / Q000302:isolation & purification* / Q000502:physiology; D010274:Parasitology / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015800:Protozoan Proteins