title: Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells.
abstract: Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.
authors: School of Agricultural Biotechnology, Seoul National University, Seoul 152-742, Republic of Korea.
authors: Samuel
authors: S
authors: Beck
authors: Xun
authors: X
authors: Jin
authors: Jinlong
authors: J
authors: Yin
authors: Sung-Hak
authors: SH
authors: Kim
authors: Nam-Kyung
authors: NK
authors: Lee
authors: Se-Yeong
authors: SY
authors: Oh
authors: Xiong
authors: X
authors: Jin
authors: Min-Kook
authors: MK
authors: Kim
authors: Eun-Bae
authors: EB
authors: Kim
authors: Jee-Soo
authors: JS
authors: Son
authors: Sung-Chan
authors: SC
authors: Kim
authors: Do-Hyun
authors: DH
authors: Nam
authors: Se-Hyuk
authors: SH
authors: Kim
authors: Sang-Kee
authors: SK
authors: Kang
authors: Hyunggee
authors: H
authors: Kim
authors: Yun-Jaie
authors: YJ
authors: Choi
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D010455:Peptides