title: Effects of dietary amino acids and repeated handling on stress response and brain monoaminergic neurotransmitters in Senegalese sole (Solea senegalensis) juveniles.
abstract: The present study aimed to assess the effects of increased availability of dietary amino acids (AA) on brain monoamine neurotransmitters and the metabolic processes resulting from stressful situations in fish. Senegalese sole (Solea senegalensis) juveniles (24.2±0.4g wet mass) were weekly subjected to an acute handling stressor (HDLG) or remained undisturbed (CTL). Additionally, both treatments were fed a control or a high protein (HP) diet (CTL, CTL HP, HDLG and HDLG HP). The HP diet slightly increased the levels of digestible indispensable AA, together with tyrosine and cysteine. Repeated handling induced a stress response after 14 and 28 days in fish held at both HDLG and HDLG HP treatments. While dietary treatment and handling stress activated the serotonergic system at 14 days, these effects were not observed after 28 days. In addition, the HP diet minimized the decrease in plasma indispensable AA due to repeated handling stress after 28 days. It was concluded that HP diet decreased post-stress plasma glucose and lactate levels in HDLG HP specimens only at 14 days of treatment. Moreover, dietary treatment was also effective in stimulating DA synthesis and release, thus dietary phenylalanine supplementation can increase DA biosynthesis in fish.
authors: Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, Faro, Portugal. bcostas@ualg.pt
authors: Benjamín
authors: B
authors: Costas
authors: Cláudia
authors: C
authors: Aragão
authors: José L
authors: JL
authors: Soengas
authors: Jesús M
authors: JM
authors: Míguez
authors: Paulo
authors: P
authors: Rema
authors: Jorge
authors: J
authors: Dias
authors: António
authors: A
authors: Afonso
authors: Luís E C
authors: LE
authors: Conceição
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism*; D005412:Flatfishes / Q000378:metabolism* / Q000502:physiology; D006854:Hydrocortisone / Q000097:blood; D009113:Muramidase / Q000097:blood; D013312:Stress, Physiological; D009435:Synaptic Transmission
chemical_list: D000596:Amino Acids; D015306:Biogenic Monoamines; D001786:Blood Glucose; D004044:Dietary Proteins; D009113:Muramidase; D006854:Hydrocortisone