title: Genomic characterization of a novel human adenovirus type 31 recombinant in the hexon gene.
abstract: A novel human recombinant adenovirus of species A (HAdV-A31 MZ) was isolated from a patient with acute gastroenteritis in Japan. The complete genome of HAdV-A31 strain MZ contains 33 776 bp. Analysis of the hexon gene of HAdV-A31 MZ indicated that its hexon sequence is the result of a genetic recombination between those of HAdV-A31 and a close relative to HAdV-A12. The recombination sites were found around the border of hypervariable loops 1 and 2 in the hexon gene, which are the most important determinants for virus neutralization. Loops 1 and 2 of this virus were genetically related to HAdV-A12, whereas all other parts of the genome were highly similar to HAdV-A31. In order to understand the evolution of adenoviruses correctly and to avoid misidentification of HAdV types, we recommend characterizing not only the hexon gene, but also the penton base and fiber genes.
authors: Division of Virology, Kawasaki City Institute of Public Health, 5-13-10 Oshima, Kawasaki-ku, Kawasaki 210-0834, Japan.
authors: Yuki
authors: Y
authors: Matsushima
authors: Division of Virology, Kawasaki City Institute of Public Health, 5-13-10 Oshima, Kawasaki-ku, Kawasaki 210-0834, Japan.
authors: Hideaki
authors: H
authors: Shimizu
authors: Blood Systems Research Institute, 270 Masonic Avenue, San Francisco, CA 94118, USA.
authors: Tung Gia
authors: TG
authors: Phan
authors: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchi Kamicho, Itabashi-ku, Tokyo 173-8610, Japan.
authors: Hiroshi
authors: H
authors: Ushijima
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D019143:Evolution, Molecular; D016679:Genome, Viral; D023281:Genomics; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus