title: Multiscale-tailored bioelectrode surfaces for optimized catalytic conversion efficiency.
abstract: We describe the elaboration of a multiscale-tailored bioelectrocatalytic system. The combination of two enzymes, D-sorbitol dehydrogenase and diaphorase, is studied with respect to the oxidation of D-sorbitol as a model system. The biomolecules are immobilized in an electrodeposited paint (EDP) layer. Reproducible and efficient catalysis of D-sorbitol oxidation is recorded when this system is immobilized on a gold electrode modified by a self-assembled monolayer of 4-carboxy-(2,5,7-trinitro-9-fluorenylidene)malonitrile used as a mediator. The insertion of mediator-modified gold nanoparticles into the EDP film increases significantly the active surface area for the catalytic reaction, which can be further enhanced when the whole system is immobilized in macroporous gold electrodes. This multiscale architecture finally leads to a catalytic device with optimized efficiency for potential use in biosensors, bioelectrosynthesis, and biofuel cells.
authors: Université de Bordeaux , Institut des Sciences Moléculaires, Site ENSCPB, 16 Avenue Pey Berland, 33607 Pessac, France.
authors: Yémima
authors: Y
authors: Bon Saint Côme
authors: Hélène
authors: H
authors: Lalo
authors: Zhijie
authors: Z
authors: Wang
authors: Mathieu
authors: M
authors: Etienne
authors: Janine
authors: J
authors: Gajdzik
authors: Gert-Wieland
authors: GW
authors: Kohring
authors: Alain
authors: A
authors: Walcarius
authors: Rolf
authors: R
authors: Hempelmann
authors: Alexander
authors: A
authors: Kuhn
mesh_terms: D015374:Biosensing Techniques; D002384:Catalysis; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D007064:L-Iditol 2-Dehydrogenase / Q000737:chemistry*; D008954:Models, Biological; D010084:Oxidation-Reduction; D016062:Porosity; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D006046:Gold; D007064:L-Iditol 2-Dehydrogenase