title: [Use of RT-PCR and enzyme immunoassays for the specific diagnosis of Crimean hemorrhagic fever].
abstract: The objective of the investigation was to evaluate the efficiency of the RT-PCR kit "AmplySens CHF" produced by InterlabService of the Central Research Institute of Epidemiology and that of the ELISA kits made by the D. I. Ivanovsky Research Institute of Virology for the specific diagnosis of Crimean hemorrhagic fever (CHF). Examination of sera from CHF patients from the Astrakhan Region showed that positive RT-PCR results were observed in 95.2 and 37.5% on days 4-8 and 9-13 after disease onset, respectively; but they were absent on days 13-17. Positive ELISA-IgM results were found in 93% on disease days 6 to 16. A high percentage (78.9%) of positive IgG samples was seen only on days 9-16. Thus, RT-PCR has a marked efficiency in diagnosing CHF until day 8 of illness while ELISA-IgM has it on day 8 or later. ELISA-IgG can be considered to be a confirming rather than compulsory test. The findings suggest that the RT-PCR kit "AmplySens CHF" produced by InterlabService of the Central Research Institute of Epidemiology and that of the ELISA kits made by the D. I. Ivanovsky Research Institute of Virology have a pronounced sensitivity and specificity and a high efficiency when concurrently used to verify CHF in patients.
authors: N G
authors: NG
authors: Putilina
authors: A R
authors: AR
authors: Azarian
authors: I N
authors: IN
authors: Trusova
authors: P M
authors: PM
authors: Mal'kov
authors: A P
authors: AP
authors: Grishanova
authors: A I
authors: AI
authors: Kovtunov
authors: G L
authors: GL
authors: Shendo
authors: A F
authors: AF
authors: Dzharkenov
authors: T E
authors: TE
authors: Arshba
authors: V F
authors: VF
authors: Larichev
authors: A M
authors: AM
authors: Butenko
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D006479:Hemorrhagic Fever, Crimean / Q000175:diagnosis*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012426:Russia; D012680:Sensitivity and Specificity
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M