title: Motion correction for cellular-resolution multi-photon fluorescence microscopy imaging of awake head-restrained mice using speed embedded HMM.
abstract: Multi-photon fluorescence microscopy (MFM) captures high-resolution fluorescence image sequences and can be used for the intact brain imaging of small animals. Recently, it has been extended from anesthetized and head-stabilized mice to awake and head-restrained ones for in vivo neurological study. In these applications, motion correction is an important pre-processing step since brain pulsation and body movement can cause motion artifact and prevent stable serial image acquisition at such high spatial resolution. This paper proposes a speed embedded Hidden Markov model (SEHMM) for motion correction in MFM imaging of awake head-restrained mice. The algorithm extends the traditional Hidden Markov model (HMM) method by embedding a motion prediction model to better estimate the state transition probability. The novelty of the method lies in using adaptive probability to estimate the linear motion, while the state-of-the-art method assumes that the highest probability is assigned to the case with no motion. In experiments we demonstrated that SEHMM is more accurate than the traditional HMM using both simulated and real MFM image sequences.
authors: Department of Control Science and Engineering, Harbin Institute of Technology, Heilongjiang, China. taoyichen@gmail.com
authors: Taoyi
authors: T
authors: Chen
authors: Zhong
authors: Z
authors: Xue
authors: Changhong
authors: C
authors: Wang
authors: Zhenshen
authors: Z
authors: Qu
authors: Kelvin K
authors: KK
authors: Wong
authors: Stephen T C
authors: ST
authors: Wong
mesh_terms: D000465:Algorithms; D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008390:Markov Chains; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods* / Q000662:veterinary*; D009068:Movement; D059906:Neuroimaging / Q000295:instrumentation / Q000379:methods; D012149:Restraint, Physical; D014851:Wakefulness