title: Enhancement of neuronal cell adhesion by covalent binding of poly-D-lysine.
abstract: We have prepared the poly-d-lysine (PDL) bound surfaces for neuron cell culture by covalent binding between the poly-d-Lysine and substrates and investigated neuronal cell adhesion properties and cell growth morphology. The number of neuronal cell and the number of neurite per neuronal cell on PDL bound surfaces was much more than those on PDL coated surfaces and also the neuronal cells on PDL bounded surfaces survived a longer time. On the pattern of covalently bound PDL, neuronal cells and their neurites are confined within the grid line leading to patterned neuronal networks with the long-term survival.
authors: IT Convergence Technology Research Lab., ETRI, Daejeon 305-700, Republic of Korea.
authors: Yong Hee
authors: YH
authors: Kim
authors: Nam Seob
authors: NS
authors: Baek
authors: Young Hwan
authors: YH
authors: Han
authors: Myung-Ae
authors: MA
authors: Chung
authors: Sang-Don
authors: SD
authors: Jung
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D007204:Indium / Q000737:chemistry; D009474:Neurons / Q000166:cytology*; D011107:Polylysine / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000737:chemistry; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D017971:Tin Compounds; D007204:Indium; D011107:Polylysine; C047711:indium oxide; D012822:Silicon Dioxide; C045358:stannic oxide