title: Optimization of culture conditions and comparison of biomass productivity of three green algae.
abstract: Culture conditions for the mass production of three green algae, Chlorella sp., Dunaliella salina DCCBC2 and Dunaliella sp., were optimized using a response surface methodology (RSM). A central composite design was applied to investigate the effects of initial pH, nitrogen and phosphate concentrations on the cultivation of microalgae. The optimal growth conditions estimated from the design are as follows: Chlorella sp. (initial pH 7.2, ammonium 17 mM, phosphate 1.2 mM), D. salina DCCBC2 (initial pH 8.0, nitrate 3.3 mM, phosphate 0.0375 mM) and Dunaliella sp. (initial pH 8.0, nitrate 3.7 mM, phosphate 0.17 mM). Culturing the microalgae with the optimized conditions confirmed that the maximum growth rates were attained for these parameters. The optimum CO(2) concentrations of Chlorella sp., D. salina DCCBC2 and Dunaliella sp. were 1.0, 3.0 and 1.0% (v/v), respectively. The specific growth rates (μ) of Chlorella sp., D. salina DCCBC2 and Dunaliella sp. were 0.58, 0.78 and 0.56 day(-1), respectively, and the biomass productivities were 0.28, 0.54 and 0.30 g dry cell wt l(-1) day(-1), respectively. The CO(2) fixation rates of Chlorella sp., D. salina DCCBC2 and Dunaliella sp. were 42.8, 90.9 and 45.5 mg l(-1) day(-1), respectively. Mixotrophic cultivation of Chlorella sp. with glucose increased biomass productivity from 0.28 to 0.51 g dry cell wt l(-1) day(-1). However, D. salina DCCBC2 and Dunaliella sp. were not stimulated by several organic compounds tested.
authors: Department of Environmental Engineering and Pioneer Research Center for Controlling of HAB, Chosun University, Gwangju, 501-759, South Korea. wonduck@chosun.ac.kr
authors: Wonduck
authors: W
authors: Kim
authors: Jang Min
authors: JM
authors: Park
authors: Geun Ho
authors: GH
authors: Gim
authors: Sang-Hwa
authors: SH
authors: Jeong
authors: Chang Min
authors: CM
authors: Kang
authors: Duk-Jin
authors: DJ
authors: Kim
authors: Si Wouk
authors: SW
authors: Kim
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D000460:Chlorophyta / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D058086:Microalgae / Q000502:physiology*; D009584:Nitrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D010710:Phosphates; D009584:Nitrogen