title: Microbial glycosylation of cardamonin by Mucor spinosus.
abstract: Microbial transformation of cardamonin by Mucor spinosus (CGMCC 3.3450) in preparative scale resulted in the isolation of two new products. Their structures were elucidated unambiguously by ESI-MS, 1H NMR, 13C NMR and 2D NMR spectra analyses as 4-O-beta-D-glucopyranosyl-6-hydroxy-2-methoxychalcone (1, 4-GluC) and 6-O-beta-D-glucopyranosyl-4-hydroxy-2-methoxychalcone (2, 6-GluC), respectively. The time-course of biotransformation by M. spinosus showed that both 4-GluC and 6-GluC appeared on the 2nd day. The optimal biotransformation temperature was 28 degrees C, the optimal biotransformation time was 72 h and the optimal concentration for cardamonin was 40 mg x mL(-1). This is the first time for successful microbial glycosylation of cardamonin in present research.
authors: Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
authors: Jing
authors: J
authors: Xu
authors: Li
authors: L
authors: Yang
authors: Shu-Juan
authors: SJ
authors: Zhao
authors: Gui-Xin
authors: GX
authors: Chou
authors: Zheng-Tao
authors: ZT
authors: Wang
mesh_terms: D001711:Biotransformation; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009089:Mucor / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C578411:4-O-beta-D-glucopyranosyl-6-hydroxy-2-methoxychalcone; C578410:6-O-beta-D-glucopyranosyl-4-hydroxy-2-methoxychalcone; D047188:Chalcones; D005960:Glucosides; C436747:cardamonin