title: Influence of matrix metalloproteinase MMP-9 on dendritic spine morphology.
abstract: An increasing body of data has shown that matrix metalloproteinase-9 (MMP-9), an extracellularly acting, Zn(2+)-dependent endopeptidase, is important not only for pathologies of the central nervous system but also for neuronal plasticity. Here, we use three independent experimental models to show that enzymatic activity of MMP-9 causes elongation and thinning of dendritic spines in the hippocampal neurons. These models are: a recently developed transgenic rat overexpressing autoactivating MMP-9, dissociated neuronal cultures, and organotypic neuronal cultures treated with recombinant autoactivating MMP-9. This dendritic effect is mediated by integrin β1 signalling. MMP-9 treatment also produces a change in the decay time of miniature synaptic currents; however, it does not change the abundance and localization of synaptic markers in dendritic protrusions. Our results, considered together with several recent studies, strongly imply that MMP-9 is functionally involved in synaptic remodelling.
authors: Department of Molecular and Cellular Neurobiology, The Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland. p.michaluk@nencki.gov.pl
authors: Piotr
authors: P
authors: Michaluk
authors: Marcin
authors: M
authors: Wawrzyniak
authors: Przemyslaw
authors: P
authors: Alot
authors: Marcin
authors: M
authors: Szczot
authors: Paulina
authors: P
authors: Wyrembek
authors: Katarzyna
authors: K
authors: Mercik
authors: Nikolay
authors: N
authors: Medvedev
authors: Ewa
authors: E
authors: Wilczek
authors: Mathias
authors: M
authors: De Roo
authors: Werner
authors: W
authors: Zuschratter
authors: Dominique
authors: D
authors: Muller
authors: Grzegorz M
authors: GM
authors: Wilczynski
authors: Jerzy W
authors: JW
authors: Mozrzymas
authors: Michael G
authors: MG
authors: Stewart
authors: Leszek
authors: L
authors: Kaczmarek
authors: Jakub
authors: J
authors: Wlodarczyk
mesh_terms: D000818:Animals; D048430:Cell Shape; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D049229:Dendritic Spines / Q000378:metabolism / Q000502:physiology*; D057075:Enzyme Assays; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000378:metabolism; D061251:Primary Cell Culture; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D046509:Tissue Culture Techniques
chemical_list: C113688:Bsn protein, rat; D019012:Integrin beta1; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D020780:Matrix Metalloproteinase 9