title: Enhanced production of ansamitocin P-3 by addition of Mg2+ in fermentation of Actinosynnema pretiosum.
abstract: The effect of divalent metal ions (i.e., Mn(2+), Mg(2+), Zn(2+), Cu(2+), and Co(2+)) on the production of anticancer ansamitocin P-3 (AP-3) by submerged cultures of Actinosynnema pretiosum in medium containing agro-industrial residues was investigated, and Mg(2+) was found to be the most effective. Under the optimal condition of Mg(2+) addition, the maximal AP-3 production titer reached 85 mg/L, which was 3.0-fold that of the control. The activities of methylmalonyl-CoA carboxyltransferase (MCT) and methylmalonyl-CoA mutase (MCM) were enhanced. The content of two precursors, malonyl-CoA and methylmalonyl-CoA, was lower than that of control. This work demonstrates that Mg(2+) addition is a simple and effective strategy for increasing AP-3 production through the regulation of enzyme activity and pools of precursors. The information obtained can be helpful to its efficient production on large scale.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors: Yongliang
authors: Y
authors: Jia
authors: Jian-Jiang
authors: JJ
authors: Zhong
mesh_terms: D000192:Actinomycetales / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D007477:Ions; D008274:Magnesium / Q000494:pharmacology*; D008316:Malonyl Coenzyme A / Q000378:metabolism; D008453:Maytansine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008670:Metals / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000214:Acyl Coenzyme A; D007477:Ions; D008670:Metals; C015357:methylmalonyl-coenzyme A; D008453:Maytansine; D008316:Malonyl Coenzyme A; C015682:ansamitocins; D008274:Magnesium