title: Lipopolysaccharide structures of Helicobacter pylori wild-type strain 26695 and 26695 HP0826::Kan mutant devoid of the O-chain polysaccharide component.
abstract: We describe a re-investigation of the structure of the lipopolysaccharide (LPS) from Helicobacter pylori genomic strain 26695 and its corresponding HP0826::Kan mutant lacking the O-chain component based on the in-depth NMR analysis of the oligosaccharide products obtained through the use of various degradation procedures performed on the purified LPS from both strains, as well as CE-MS data. New structural evidence indicates the presence of the linear arrangement of glucan and heptan portions of the LPS attached through -6-α-DDHep-3-α-L-Fuc-3-β-GlcNAc- fragment to the inner core DD-heptose residue. This structure differs from previously reported structures of the H. pylori 26695 LPS in several aspects.
authors: Institute for Biological Sciences, National Research Council Canada, Ottawa, Canada.
authors: Eleonora
authors: E
authors: Altman
authors: Vandana
authors: V
authors: Chandan
authors: Jianjun
authors: J
authors: Li
authors: Evgeny
authors: E
authors: Vinogradov
mesh_terms: D002240:Carbohydrate Sequence; D016480:Helicobacter pylori / Q000737:chemistry* / Q000235:genetics; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009154:Mutation; D010084:Oxidation-Reduction
chemical_list: D008070:Lipopolysaccharides