title: Boron-dependent degradation of NIP5;1 mRNA for acclimation to excess boron conditions in Arabidopsis.
abstract: Boron (B) is an essential plant micronutrient that is toxic at higher levels. NIP5;1 is a boric acid channel required for B uptake and growth under B deficiency. Accumulation of the NIP5;1 transcript is upregulated under B deficiency in Arabidopsis thaliana roots. To elucidate the mechanism of regulation, the 5' untranslated region (UTR) of NIP5;1 was tested for its ability to confer B-dependent regulation using β-glucuronidase and green fluorescent protein as reporters. This analysis showed that the 5' UTR was involved in NIP5;1 transcript accumulation in response to B conditions. We also found that high-B conditions trigger NIP5;1 mRNA degradation and that the sequence from +182 to +200 bp in the 5' UTR is required for this mRNA destabilization. In the nip5;1-1 mutant background, a NIP5;1 complementation construct without the 5' UTR produced high levels of mRNA accumulation, increased B concentrations in tissues, and reduced growth under high-B conditions. These data suggest that the 5' UTR controls B-dependent NIP5;1 mRNA degradation and that NIP5;1 mRNA degradation is important for plant acclimation to high-B conditions.
authors: Graduate School of Agricultural and Life Science, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors: Mayuki
authors: M
authors: Tanaka
authors: Junpei
authors: J
authors: Takano
authors: Yukako
authors: Y
authors: Chiba
authors: Fabien
authors: F
authors: Lombardo
authors: Yuki
authors: Y
authors: Ogasawara
authors: Hitoshi
authors: H
authors: Onouchi
authors: Satoshi
authors: S
authors: Naito
authors: Toru
authors: T
authors: Fujiwara
mesh_terms: D020121:5' Untranslated Regions; D000064:Acclimatization; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001895:Boron / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism
chemical_list: D020121:5' Untranslated Regions; D020346:Aquaporins; D029681:Arabidopsis Proteins; C512214:NIP5;1 protein, Arabidopsis; D012333:RNA, Messenger; D018749:RNA, Plant; D001895:Boron