title: Fibulin-3 promoter methylation alters the invasive behavior of non-small             cell lung cancer cell lines via MMP-7 and MMP-2 regulation.
abstract: Fibulin-3, an extracellular glycoprotein, has been suggested as having functions             in tissue regeneration and organogenesis. However, its role in cancer remains             unclear. We show here that fibulin-3 was silenced by hypermethylation of the promoter             region in the relatively invasive A549 non-small cell lung cancer (NSCLC) cells             compared with less invasive H460 NSCLC cells. Enforced expression of fibulin-3             in A549 cells down-regulated cellular MMP-7 and MMP-2, which was followed by inhibition             of cell invasiveness. Conversely, suppression of fibulin-3 expression with siRNA             in H460 cells showed the opposite effect. These results indicate that fibulin-3             is a negative regulator of invasiveness in NSCLC and further studies are needed             for its therapeutic applications in treatment of NSCLC.
authors: Department of Radiation Biology, Environmental Radiation Research                     Group, Korea Atomic Energy Research Institute, 1045 Daedeok-Daero, Yusong-gu,                     Daejeon 305-353, Republic of Korea.
authors: Eun Jin
authors: EJ
authors: Kim
authors: So Yong
authors: SY
authors: Lee
authors: Mi Kyung
authors: MK
authors: Woo
authors: Soo Im
authors: SI
authors: Choi
authors: Tae Rim
authors: TR
authors: Kim
authors: Min-Jung
authors: MJ
authors: Kim
authors: Kug Chan
authors: KC
authors: Kim
authors: Eun Wie
authors: EW
authors: Cho
authors: In Gyu
authors: IG
authors: Kim
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D019175:DNA Methylation; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic; D015398:Signal Transduction
chemical_list: D017077:Culture Media, Conditioned; C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9