title: [Screening the serum protein biomarker of patients with sepsis by cytokine antibody chips].
abstract: OBJECTIVE
  To investigate the expression of inflammatory cytokines in patients with sepsis by cytokine antibody chips.
  
  
  METHODS
  To screen the protein biomarker for rapidly diagnosing sepsis, 79 cytokines in 9 cases of patients with sepsis and 4 cases of healthy control were measured using cytokine antibody chip. Analyse the signaling values of these 79 cytokines using SAM (significance analysis of microarray, SAM) software.
  
  
  RESULTS
  By SAM analysis: insulin like growth factor binding protein-1 (IGFBP-1), epidermal growth factor (HGF), osteopotin, insulin like growth factor binding protein-4 (IGFBP-4), interferon inducible protein-10 (IP-10) and B-lymphocyte chemoattractant (BLC) were identified to be highly expressed in the sepsis while platelet-derived growth factor-BB (PDGF-BB), brain-derived neurotrophic (BDNF), macrophage inflammatory protein-1β (MIP-1β), interleukin-8 (IL-8), epidermal growth factor (EGF) and interleukin-1β(IL-1β)lowly expressed. Cluster analysis of significantly expressed cytokines showed that the sepsis and the control formed distinctly separate groups.
  
  
  CONCLUSIONS
  Antibody chips demonstrate a significant change in sepsis patients, screening the protein biomarker for rapidly diagnosing sepsis is feasible.
authors: General Hospital of Guangzhou Command, Guangzhou 510010, China. shiyuling6@sina.com
authors: Yu-Ling
authors: YL
authors: Shi
authors: Ta
authors: T
authors: Hu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D015415:Biomarkers / Q000097:blood; D016000:Cluster Analysis; D016207:Cytokines / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D040901:Proteomics; D018805:Sepsis / Q000175:diagnosis*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D016207:Cytokines