title: The colorimetric detection and quantitation of total protein.
abstract: Protein quantification is an important step for handling protein samples for isolation and characterization; it is a prerequisite step before submitting proteins for chromatographic, electrophoretic, or immunochemical analysis and separation. Colorimetric methods are fast, simple, and not laborious. This unit describes a number of assays able to detect protein concentrations in the low microgram to milligram per milliliter ranges in a variety of formats.
authors: Pierce Biotechnology Inc., Rockford, Illinois, USA.
authors: Randall I
authors: RI
authors: Krohn
mesh_terms: D001738:Biuret Reaction / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D011506:Proteins; D011804:Quinolines; D012394:Rosaniline Dyes; C047117:bicinchoninic acid; C004692:coomassie Brilliant Blue