title: Methanobacterium lacus sp. nov., isolated from the profundal sediment of a freshwater meromictic lake.
abstract: An autotrophic, hydrogenotrophic methanogen, designated strain 17A1(T), was isolated from the profundal sediment of the meromictic Lake Pavin, France. The cells of the novel strain, which were non-motile, Gram-staining-negative rods that measured 2-15 µm in length and 0.2-0.4 µm in width, grew as filaments. Strain 17A1(T) grew in a mineral medium and its growth was stimulated by the addition of yeast extract, vitamins, acetate or rumen fluid. Penicillin, vancomycin and kanamycin reduced growth but did not completely inhibit it. Growth occurred at 14-41 °C (optimum 30 °C), at pH 5.0-8.5 (optimum pH 6.5) and with 0-0.4 M NaCl (optimum 0.1 M). The novel strain utilized H(2)/CO(2) and methanol/H(2) as substrates but not formate, acetate, methylamine/H(2), isobutanol or 2-propanol. Its genomic DNA G+C content was 37.0 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain 17A1(T) appeared to be a member of the genus Methanobacterium, with Methanobacterium beijingense 8-2(T) (96.3% sequence similarity) identified as the most closely related established species. Based on phenotypic and phylogenetic data, strain 17A1(T) represents a novel species of methanogen within the genus Methanobacterium, for which the name Methanobacterium lacus sp. nov. is proposed. The type strain is 17A1(T) (=DSM 24406(T)=JCM 17760(T)).
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Guillaume
authors: G
authors: Borrel
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Keith
authors: K
authors: Joblin
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Annie
authors: A
authors: Guedon
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Jonathan
authors: J
authors: Colombet
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Vincent
authors: V
authors: Tardy
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Anne-Catherine
authors: AC
authors: Lehours
authors: UMR CNRS 6023, Université Blaise Pascal, BP 10448, F-63000 Clermont-Ferrand, France.|Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, BP 10448, F-63000 Clermont-Ferrand, France.
authors: Gérard
authors: G
authors: Fonty
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005602:France; D005618:Fresh Water; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D017014:Methanobacterium / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride