title: Molecular characterization of alternative transcripts of the horse BMAL1 gene.
abstract: The horse BMAL1 gene encodes the brain and muscle Arnt-like protein 1, which is a key regulator of circadian rhythmic systems in most organs and cells. The first exon of the horse-specific BMAL1 gene is produced by an exonization event of LINE3 (CR1) and SINE (MIR) was detected by bioinformatic analysis. Alternative variants generated by cassette exon event in various horse tissues were also detected by RT-PCR amplification and sequencing. The cDNA sequences of the horse transcripts (BMAL1a, BMAL1b) contain additional 21 bp and 71 bp fragments relative to horse BMAL1. Quantitative real-time RT-PCR was performed to compare the expression patterns between transcript variants in various horse tissues. The results of these experiments showed splice variants that were widely expressed in most tissues. Furthermore, they were highly expressed in cerebellum, heart, and kidney.
authors: Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors: Jin-Han
authors: JH
authors: Bae
authors: Kung
authors: K
authors: Ahn
authors: Gyu-Hwi
authors: GH
authors: Nam
authors: Chong-Eon
authors: CE
authors: Lee
authors: Kyung-Do
authors: KD
authors: Park
authors: Hak-Kyo
authors: HK
authors: Lee
authors: Byung-Wook
authors: BW
authors: Cho
authors: Heui-Soo
authors: HS
authors: Kim
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics / Q000378:metabolism*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D006736:Horses / Q000235:genetics* / Q000378:metabolism*; D058977:Molecular Sequence Annotation
chemical_list: D056930:ARNTL Transcription Factors; D018076:DNA, Complementary