title: [Screening of single-chain variable fragment (scFv) to bone sialoprotein].
abstract: OBJECTIVE
  To select single-chain variable fragment(scFv) antibody specific for bone sialoprotein(BSP) from Human Single Fold scFv Libraries.
  
  
  METHODS
  Human Single Fold scFv Libraries were panned against immobilized BSP in a microtiter plate, after three rounds of panning, 96 clones were determined specific to BSP. The specificity of each scFv clone was determined by ELISA. The coding gene for BSP protein scFv has been sequenced.
  
  
  RESULTS
  Phage antibody for BSP protein had a specific combination character. There were 368 bp, 527 bp, 935 bp which werer light chain, heavy chain and joint gene fragment with the resuLt of PCR. The DNA sequence data showed that there were 11 differences of the amino acids in the light chain, while there were only 3 differences in the heavy chain of scFv.
  
  
  CONCLUSIONS
  scFv specific to BSP has been identified by means of phage display technology.
authors: Department of Medical Research, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China. wjiangtao80@126.com
authors: Jiang-Tao
authors: JT
authors: Wang
authors: Jie
authors: J
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000918:Antibody Specificity / Q000235:genetics; D006801:Humans; D050440:Immunoglobulin Subunits / Q000235:genetics; D058574:Integrin-Binding Sialoprotein / Q000276:immunology* / Q000378:metabolism; D019151:Peptide Library; D057127:Single-Chain Antibodies / Q000276:immunology* / Q000378:metabolism
chemical_list: D050440:Immunoglobulin Subunits; D058574:Integrin-Binding Sialoprotein; D019151:Peptide Library; D057127:Single-Chain Antibodies