title: Antibacterial effects of chemomechanical instrumentation and calcium hydroxide in primary teeth with pulp necrosis.
abstract: OBJECTIVE
  This study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of Fusobacterium nucleatum and black-pigmented rods in the canals of these teeth.
  
  
  METHODS
  Microbiological sample collections (MSCs) were obtained: after coronal access to the canals of primary incisors (in 18 teeth; MSC 1); after chemomechanical instrumentation (in 10 teeth; MSC 2); and 72 hours after removal of intracanal medication (in 18 teeth; MSC 3). These samples were sent for microbiological processing. The results were analyzed statistically via chi-square, analysis of variance, and Games-Howell tests (P<.05).
  
  
  RESULTS
  Micro-organisms were isolated in approximately 94%, 10%, and 83% of canals, respectively, in MSCs 1, 2, and 3. There was a statistically significant difference only between MSCs 1 and 2 (P<.03). F nucleatum and black-pigmented rods were detected in approximately 56% and 11% of canals in MSC 1, respectively.
  
  
  CONCLUSIONS
  Chemomechanical instrumentation and calcium hydroxide-based dressing have an anti-bacterial effect by significantly reducing the number of micro-organisms in the main root canal. They showed a limited efficacy, however, and did not prevent bacterial regrowth after endodontic therapy in primary teeth with pulp necrosis secondary to trauma.
authors: Department of Dental Clinics, School of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
authors: Denise Lins
authors: DL
authors: de Sousa
authors: Rebecca Bastos Rocha Araújo
authors: RB
authors: de Sousa
authors: Daniela Nunes
authors: DN
authors: Pinto
authors: José Jeová Siebra Moreira
authors: JJ
authors: Neto
authors: Cibele Barreto Mano
authors: CB
authors: de Carvalho
authors: Paulo César
authors: PC
authors: de Almeida
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D002710:Chlorhexidine / Q000494:pharmacology; D003430:Cross-Sectional Studies; D003790:Dental Pulp Necrosis / Q000209:etiology / Q000382:microbiology* / Q000628:therapy; D005260:Female; D016967:Fusobacterium nucleatum / Q000187:drug effects*; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000187:drug effects*; D006801:Humans; D008297:Male; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation / Q000379:methods*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D018709:Statistics, Nonparametric; D018677:Tooth Injuries / Q000150:complications; D014094:Tooth, Deciduous
chemical_list: D000891:Anti-Infective Agents, Local; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002126:Calcium Hydroxide; D002710:Chlorhexidine