title: Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors.
abstract: BACKGROUND
  Reports of Monosomy 7 in patients receiving granulocyte-colony-stimulating factor (G-CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G-CSF produces Monosomy 7 aneuploidy in healthy donors.
  
  
  METHODS
  We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G-CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G-CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.
  
  
  RESULTS
  We found no abnormalities in Chromosomes 7 and 8 in G-CSF-mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G-CSF- and Dex-treated donors.
  
  
  CONCLUSIONS
  G-CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.
authors: Hematology Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA. olnesmj@nhlbi.nih.gov
authors: Matthew J
authors: MJ
authors: Olnes
authors: Andrea
authors: A
authors: Poon
authors: Susan J
authors: SJ
authors: Miranda
authors: Loretta
authors: L
authors: Pfannes
authors: Zachary
authors: Z
authors: Tucker
authors: Kelsey
authors: K
authors: Loeliger
authors: Hesed
authors: H
authors: Padilla-Nash
authors: Yu Ying
authors: YY
authors: Yau
authors: Thomas
authors: T
authors: Ried
authors: Susan F
authors: SF
authors: Leitman
authors: Neal S
authors: NS
authors: Young
authors: Elaine M
authors: EM
authors: Sloand
mesh_terms: D000782:Aneuploidy; D002872:Chromosome Deletion; D002897:Chromosomes, Human, Pair 7 / Q000187:drug effects; D003907:Dexamethasone / Q000008:administration & dosage; D042822:Genomic Instability / Q000187:drug effects*; D005938:Glucocorticoids / Q000008:administration & dosage; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D006098:Granulocytes / Q000187:drug effects* / Q000502:physiology / Q000637:transplantation; D019650:Hematopoietic Stem Cell Mobilization / Q000009:adverse effects / Q000379:methods* / Q000592:standards; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D014019:Tissue Donors
chemical_list: D005938:Glucocorticoids; D016179:Granulocyte Colony-Stimulating Factor; D003907:Dexamethasone