title: Phosphoenolpyruvate carboxykinase in cherry (Prunus avium L.) fruit during development.
abstract: In this study the abundance and location of phosphoenolpyruvate carboxykinase (PEPCK) was determined in the flesh and skin of the sweet cherry (Prunus avium L.) cultivar Durone Nero II during development. PEPCK was not present in young fruit but appeared in both tissues as the fruit increased in size. In these there was no net dissimilation of malic acid, which accounts for the bulk of their organic acid contents when PEPCK was present. To assist in understanding the function of PEPCK, the abundance of a number of other enzymes was determined. These enzymes were aspartate aminotransferase (AspAT), glutamine synthetase (GS), phosphoenolpyruvate carboxylase (PEPC), pyruvate, orthophosphate dikinase (PPDK), and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). A potential role for PEPCK in the regulation of pH and the utilization of malate in gluconeogenesis in the flesh and skin of cherries is presented.
authors: Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno, 74, I-06121, Perugia, Italy. rob.walker@talktalk.net
authors: Robert P
authors: RP
authors: Walker
authors: Alberto
authors: A
authors: Battistelli
authors: Stefano
authors: S
authors: Moscatello
authors: Zhi-Hui
authors: ZH
authors: Chen
authors: Richard C
authors: RC
authors: Leegood
authors: Franco
authors: F
authors: Famiani
mesh_terms: D005638:Fruit / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008293:Malates / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism*; D027861:Prunus / Q000201:enzymology* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D008293:Malates; C030298:malic acid; D010730:Phosphoenolpyruvate Carboxylase