title: Fibrosis of pulmonary vascular remodeling in carotid artery-jugular vein shunt pulmonary artery hypertension model of rats.
abstract: OBJECTIVE
  The aim of the present study was to observe the changes of hemodynamics, stereology in pulmonary vascular remodeling and messenger RNA (mRNA) expressions of transforming growth factor beta 1, and receptors in carotid artery-jugular vein (CA-JV) shunt pulmonary artery hypertension model of rats.
  
  
  METHODS
  Thirty-six Sprague-Dawley rats were randomized into three groups: CA-JV group, monocrotaline (MCT) administration group, and control group. Left CA-JV shunts were established in CA-JV group. Dorsal subcutaneous injections of MCT (60 mg kg(-1)) were received in MCT group. Ligations of left common carotid artery and external jugular vein were performed in control group. Right ventricular systolic pressure (RVSP) measurement, histological evaluation of the pulmonary tissue, and mRNA levels of transforming growth factor beta 1 (TGFß1), receptor 1 and receptor 2, were investigated after 6 weeks on MCT group, and after 12 weeks on both control and CA-JV groups.
  
  
  RESULTS
  Compared with control group, RVSP, percentage of fibrous tissue (F%) in pulmonary arterioles, mRNA levels of TGFß1, and receptors of CA-JVand MCT groups increased significantly. Severe hemodynamics change was found in MCT groups. On the other hand, CA-JV group demonstrated more obvious fibrogenesis and TGFß1 signals' upregulation in two pulmonary artery hypertension (PAH) models.
  
  
  CONCLUSIONS
  CA-JV shunt model of rats was a well-established PAH animal model simulating congenital heart disease with systemic-pulmonary shunt.
authors: Department of Cardiac Surgery, the 1st Affiliated Hospital of Sun Yat-Sen University, Guangzhou, PR China.
authors: Mai
authors: M
authors: Xiong
authors: Jian-Ping
authors: JP
authors: Yao
authors: Zhong-Kai
authors: ZK
authors: Wu
authors: Bing
authors: B
authors: Liao
authors: Ying-Jie
authors: YJ
authors: Liang
authors: Xi
authors: X
authors: Zhang
authors: Zhi-Ping
authors: ZP
authors: Wang
mesh_terms: D000818:Animals; D001160:Arterioles / Q000473:pathology; D001166:Arteriovenous Shunt, Surgical / Q000379:methods; D017536:Carotid Artery, Common / Q000601:surgery; D004195:Disease Models, Animal; D005355:Fibrosis; D005786:Gene Expression Regulation / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology; D006976:Hypertension, Pulmonary / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007601:Jugular Veins / Q000601:surgery; D008297:Male; D011651:Pulmonary Artery / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D018125:Receptors, Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D018125:Receptors, Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1