title: Production of lipases by solid state fermentation using vegetable oil-refining wastes.
abstract: Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45°C for 20 days) in 4.5-L reactors. The lipases were extracted from the solid medium using 100mM Tris-HCl, pH 8.0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61-65°C and at pH 7.7-9. For the solid samples, the lipolytic activity reached up to 120,000 UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts.
authors: Composting Research Group, Department of Chemical Engineering, Escola d'Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, 08193 Barcelona, Spain.
authors: Angélica
authors: A
authors: Santis-Navarro
authors: Teresa
authors: T
authors: Gea
authors: Raquel
authors: R
authors: Barrena
authors: Antoni
authors: A
authors: Sánchez
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002021:Buffers; D004795:Enzyme Stability / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007220:Industrial Waste / Q000032:analysis*; D008049:Lipase / Q000096:biosynthesis*; D008066:Lipolysis / Q000187:drug effects; D010938:Plant Oils / Q000737:chemistry*; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature
chemical_list: D002021:Buffers; D007220:Industrial Waste; D010938:Plant Oils; D013501:Surface-Active Agents; D008049:Lipase