title: An efficient and novel screening model for assessing the bioactivity of extracts against multidrug-resistant Pseudomonas aeruginosa using Caenorhabditis elegans.
abstract: As a large number of multidrug-resistant bacteria have emerged, and there is an urgent need for the development of new antibacterial agents. In this study, we developed a liquid-based slow killing assay to be carried out in standard 96-well microtiter plates. This screening method was designed to facilitate high-throughput screening of small molecules and extracts. In antibiotic rescue assays, the Caenorhabditis elegans multidrug-resistant Pseudomonas aeruginosa infection model displayed a high degree of drug resistance in vivo and in vitro. We used the method to screen 1,300 extracts, and found 36 extracts (2.7%) which prolonged the survival of infected nematodes, and four (0.3%) of these extracts showed in vitro and in vivo anti-multidrug resistant P. aeruginosa activity. These results indicate that the whole-animal C. elegans multidrug-resistant bacterial model can be used to screen antibacterial compounds, and can also be useful for bioactive compounds which most likely cannot be identified in vitro.
authors: State Key Lab for New Drug and Pharmaceutical Processes, Shanghai Institute of Pharmaceutical Industry.
authors: Yu-meng
authors: YM
authors: Zhou
authors: Lei
authors: L
authors: Shao
authors: Ji-an
authors: JA
authors: Li
authors: Li-zhong
authors: LZ
authors: Han
authors: Wai-jiao
authors: WJ
authors: Cai
authors: Chun-bao
authors: CB
authors: Zhu
authors: Dai-jie
authors: DJ
authors: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000254:growth & development / Q000382:microbiology; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D057166:High-Throughput Screening Assays; D007814:Larva / Q000254:growth & development* / Q000382:microbiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D011552:Pseudomonas Infections / Q000188:drug therapy*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D054852:Small Molecule Libraries