title: Encapsulation of gold nanoparticles by simian virus 40 capsids.
abstract: Viral capsid-nanoparticle hybrid structures constitute a new type of nanoarchitecture that can be used for various applications. We previously constructed a hybrid structure comprising quantum dots encapsulated by simian virus 40 (SV40) capsids for imaging viral infection pathways. Here, gold nanoparticles (AuNPs) are encapsulated into SV40 capsids and the effect of particle size and surface ligands (i.e. mPEG and DNA) on AuNP encapsulation is studied. Particle size and surface decoration play complex roles in AuNP encapsulation by SV40 capsids. AuNPs ≥15 nm (when coated with mPEG750 rather than mPEG2000), or ≥10 nm (when coated with 10T or 50T DNA) can be encapsulated. Encapsulation efficiency increased as the size of the AuNPs increased from 10 to 30 nm. In addition, the electrostatic interactions derived from negatively charged DNA ligands on the AuNP surfaces promote encapsulation when the AuNPs have a small diameter (i.e. 10 nm and 15 nm). Moreover, the SV40 capsid is able to carry mPEG750-modified 15-nm AuNPs into living Vero cells, whereas the mPEG750-modified 15-nm AuNPs alone cannot enter cells. These results will improve our understanding of the mechanisms underlying nanoparticle encapsulation in SV40 capsids and enable the construction of new functional hybrid nanostructures for cargo delivery.
authors: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, No.44, Xiaohongshan, Wuhan 430071, P. R. China.
authors: Tingjuan
authors: T
authors: Wang
authors: Zhiping
authors: Z
authors: Zhang
authors: Ding
authors: D
authors: Gao
authors: Feng
authors: F
authors: Li
authors: Hongping
authors: H
authors: Wei
authors: Xiaosheng
authors: X
authors: Liang
authors: Zongqiang
authors: Z
authors: Cui
authors: Xian-En
authors: XE
authors: Zhang
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D013539:Simian virus 40 / Q000378:metabolism*; D014709:Vero Cells
chemical_list: D036022:Capsid Proteins; D011092:Polyethylene Glycols; D006046:Gold; D004247:DNA