title: Structome of Saccharomyces cerevisiae determined by freeze-substitution and serial ultrathin-sectioning electron microscopy.
abstract: The cell structure has been studied using light and electron microscopies for centuries, and it is assumed that the whole structure is clarified by now. Little quantitative and three-dimensional analysis of cell structure, however, has been undertaken. We have coined a new word, 'structome', by combining 'structure' and '-ome', and defined it as the 'quantitative and three-dimensional structural information of a whole cell at the electron microscopic level'. In the present study, we performed structome analysis of Saccharomyces cerevisiae, one of the most widely researched biological materials, by using freeze-substitution and serial ultrathin-sectioning electron microscopy. Our analysis revealed that there were one to three mitochondria, ~220 000 ribosomes in a cell, and 13-28 endoplasmic reticula/Golgi apparatus which do not form networks in the cytoplasm in the G1 phase. Nucleus occupied ~10.5% of the cell volume; cell wall occupied ~17%; vacuole occupied ~5.8%; cytoplasm occupied ~64%; and mitochondria occupied only ~1.7% in the G1 phase. Structome analysis of cells would form a base for the post-genome research.
authors: Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, Japan.
authors: Masashi
authors: M
authors: Yamaguchi
authors: Yuichi
authors: Y
authors: Namiki
authors: Hitoshi
authors: H
authors: Okada
authors: Yuko
authors: Y
authors: Mori
authors: Hiromitsu
authors: H
authors: Furukawa
authors: Jinfang
authors: J
authors: Wang
authors: Misako
authors: M
authors: Ohkusu
authors: Susumu
authors: S
authors: Kawamoto
mesh_terms: D002467:Cell Nucleus / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D022082:Cellular Structures / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D017110:Freeze Substitution / Q000379:methods*; D016193:G1 Phase; D006056:Golgi Apparatus / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000648:ultrastructure*; D014617:Vacuoles / Q000648:ultrastructure