title: Methylophaga lonarensis sp. nov., a moderately haloalkaliphilic methylotroph isolated from the soda lake sediments of a meteorite impact crater.
abstract: A moderately haloalkaliphilic methylotrophic bacterium possessing the ribulose monophosphate pathway for carbon assimilation, designated MPL(T), was isolated from Lonar Lake sediment microcosms that were oxidizing methane for two weeks. The isolate utilized methanol and was an aerobic, Gram-negative, asporogenous, motile, short rod that multiplied by binary fission. The isolate required NaHCO(3) or NaCl for growth and, although not auxotrophic for vitamin B(12), had enhanced growth with vitamin B(12). Optimal growth occurred with 0.5-2% (w/v) NaCl, at 28-30 °C and at pH 9.0-10.0. The cellular fatty acid profile consisted primarily of straight-chain saturated C(16:0) and unsaturated C(16:1)ω7c and C(18:1)ω7c. The major ubiquinone was Q-8. The dominant phospholipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Cells accumulated ectoine as the main compatible solute. The DNA G+C content was 50.0 mol%. The isolate exhibited 94.0-95.4% 16S rRNA gene sequence similarity with the type strains of methylotrophs belonging to the genus Methylophaga and 31% DNA-DNA relatedness with the reference strain, Methylophaga alcalica VKM B-2251(T). It is proposed that strain MPL(T) represents a novel species, Methylophaga lonarensis sp. nov. (type strain MPL(T)=VKM B-2684(T)=MCC 1002(T)).
authors: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, India.
authors: Chakkiath Paul
authors: CP
authors: Antony
authors: G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow 142290, Russia.
authors: Nina V
authors: NV
authors: Doronina
authors: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
authors: Rich
authors: R
authors: Boden
authors: G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow 142290, Russia.
authors: Yuri A
authors: YA
authors: Trotsenko
authors: Microbial Culture Collection, National Centre for Cell Science, Pune 411007, India.
authors: Yogesh S
authors: YS
authors: Shouche
authors: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
authors: J Colin
authors: JC
authors: Murrell
mesh_terms: D001482:Base Composition; D002244:Carbon / Q000378:metabolism; D002254:Carbonates / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D060106:Lakes; D008124:Locomotion; D053858:Metabolic Networks and Pathways; D019110:Meteoroids; D008697:Methane / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010429:Pentoses / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D044147:Piscirickettsiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014451:Ubiquinone / Q000032:analysis; D014805:Vitamin B 12 / Q000378:metabolism
chemical_list: D002254:Carbonates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010429:Pentoses; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D012965:Sodium Chloride; C005686:sodium carbonate; C100182:ribulose; D002244:Carbon; D008697:Methane; D014805:Vitamin B 12