title: Effect of partial and complete blockade of vanilloid (TRPV1-6) and ankyrin (TRPA1) transient receptor potential ion channels on urinary bladder motor activity in an experimental hyperosmolar overactive bladder rat model.
abstract: The study investigated the mechanisms through which the hyperosmolarity might induce detrusor overactivity (DO). We compared the bladder activity in response to partial and complete blockade of TRPV1-6 and TRPA1 receptors. Experiments were performed on 42 rats. DO was induced by using hyperosmolar saline. All animals were randomly divided into six groups. The measurements represent the average of five bladder micturition cycles. Hyperosmolar saline induced DO. The complete blockade of TRPV1-6 and TRPA1 prevented DO. The partial blockade of TRPV1 didn't prevented DO. In the voiding phase periodical bladder contractions complexes occurred leading to slow urine flow due to bladder distension. Ruthenium red and capsaicin resulted in complete disorganisation of detrusor muscle contractility impairing urine voiding and leading to constantly lasting urine retention in healthy rats.
  
  
  CONCLUSIONS
  hyperosmolar-induced DO is mediated by TRPV and TRPA1 channels; the hyperosmolar stimuli of urinary bladder might be transmitted mostly via ruthenium red sensitivity pathway.
authors: Department of Pathophysiology, Jagiellonian University, Medical College, Cracow, Poland. kajus13@poczta.onet.pl
authors: K
authors: K
authors: Juszczak
authors: A
authors: A
authors: Ziomber
authors: P J
authors: PJ
authors: Thor
mesh_terms: D000283:Administration, Intravesical; D000818:Animals; D017487:Ankyrins / Q000378:metabolism; D002211:Capsaicin / Q000008:administration & dosage; D004195:Disease Models, Animal; D005260:Female; D009994:Osmolar Concentration; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012430:Ruthenium Red / Q000008:administration & dosage; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001743:Urinary Bladder / Q000502:physiology*; D053201:Urinary Bladder, Overactive / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D016055:Urinary Retention / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*
chemical_list: D017487:Ankyrins; D012462:Saline Solution, Hypertonic; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C480976:Trpa1 protein, rat; D012430:Ruthenium Red; D002211:Capsaicin