title: Lymphogranuloma venereum variant L2b-specific polymerase chain reaction: insertion used to close an epidemiological gap.
abstract: The management of the ongoing lymphogranuloma venereum epidemic in industrialized Western countries caused by Chlamydia trachomatis variant L2b still needs improvements in diagnosis, therapy and prevention. We therefore developed the first rapid C. trachomatis variant L2b-specific polymerase chain reaction to circumvent laborious ompA gene sequencing.
authors: Laboratory of Immunogenetics, Department of Pathology, University Hospital L. Sacco, Milano, Italy.
authors: S P
authors: SP
authors: Verweij
authors: A
authors: A
authors: Catsburg
authors: S
authors: S
authors: Ouburg
authors: A
authors: A
authors: Lombardi
authors: R
authors: R
authors: Heijmans
authors: F
authors: F
authors: Dutly
authors: R
authors: R
authors: Frei
authors: S A
authors: SA
authors: Morré
authors: D
authors: D
authors: Goldenberger
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003429:Cross Reactions; D017931:DNA Primers / Q000235:genetics; D005060:Europe; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000175:diagnosis* / Q000382:microbiology*; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; C410162:PmpH protein, Chlamydia trachomatis