title: Fibronectin and bone morphogenetic protein-2-decorated poly(OEGMA-r-HEMA) brushes promote osseointegration of titanium surfaces.
abstract: To be better used as medical implants in orthopedic and dental clinical applications, titanium and titanium-based alloys need to be capable of inducing osteogenesis. Here we describe a method that allows the facile decoration of titanium surfaces to impart an osteogenesis capacity. A Ti surface was first deposited on a poly(OEGMA-r-HEMA) film using surface-initiated atom-transfer radical polymerization (SI-ATRP) with the further step of carboxylation. The modified surfaces were resistant to cell adhesion. Fibronectin (FN) and recombinant human bone morphogenetic protein-2 (rhBMP-2) were further immobilized onto p(OEGMA-r-HEMA) matrices. Our results demonstrate that the FN- and rhBMP-2-conjugated polymer surfaces could induce the adhesion of MC3T3 cells on Ti surfaces. Moreover, the protein-tethered surface exhibited enhanced cell differentiation in terms of alkaline phosphatase activity compared to that of the pristine Ti surface at similar cell proliferation rates. This research establishes a simple modification method of Ti surfaces via Ti-thiolate self-assembled monolayers (SAMs) and SI-ATRP and identifies a dual-functional Ti surface that combines antifouling and osseointegration promotion.
authors: Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, School and Hospital of Stomatology, Peking University, 100871 Beijing, PR China.
authors: Xiaoshuai
authors: X
authors: Ren
authors: Yuanzi
authors: Y
authors: Wu
authors: Yan
authors: Y
authors: Cheng
authors: Hongwei
authors: H
authors: Ma
authors: Shicheng
authors: S
authors: Wei
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry*; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D010012:Osteogenesis; D011092:Polyethylene Glycols / Q000737:chemistry*; D011109:Polymethacrylic Acids; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D055396:Bone Morphogenetic Protein 2; D005353:Fibronectins; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011994:Recombinant Proteins; C528061:poly(poly(ethylene glycol)methacrylate); D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate; D014025:Titanium