title: The impact of Trichinella spiralis excretory-secretory products on dendritic cells.
abstract: Parasitic nematode Trichinella spiralis exert immunomodulatory effect on the host immune response through excretory-secretory products (ES L1) released from the encysted muscle larvae. Rat bone-marrow derived dendritic cells (DCs) stimulated with ES L1 antigens acquire semi-matured status and induce Th2 and regulatory responses in vitro and in vivo. Priming naïve T cells in vitro with ES L1 pulsed DCs caused strong Th2 polarization, accompanied by elevated production of regulatory cytokines IL-10 and TGF-β and no increase in the proportion of CD4+CD25+Foxp3+ among the effector T cell population. In vivo T cell priming resulted in mixed Th1/Th2 cytokine response, with the dominance of the Th2 type and elevated levels of regulatory cytokines. Significant increase in the proportion of CD4+CD25+Foxp3+ cells was found among recipient's spleen cells. We have achieved to create immune status characteristic for the live infection by in vivo application of DCs educated with ES L1 antigens.
authors: Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia. alisa@inep.co.rs
authors: A
authors: A
authors: Gruden-Movsesijan
authors: N
authors: N
authors: Ilic
authors: M
authors: M
authors: Colic
authors: I
authors: I
authors: Majstorovic
authors: S
authors: S
authors: Vasilev
authors: I
authors: I
authors: Radovic
authors: Lj
authors: Lj
authors: Sofronic-Milosavljevic
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology; D018791:CD4 Lymphocyte Count; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015801:Helminth Proteins / Q000276:immunology*; D007111:Immunity, Cellular; D016753:Interleukin-10 / Q000276:immunology; D007814:Larva / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D008213:Lymphocyte Activation; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000276:immunology; D017160:Trichinella spiralis / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D014235:Trichinellosis / Q000276:immunology / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D016212:Transforming Growth Factor beta; C097419:excretory-secretory antigen, Trichinella; D016753:Interleukin-10