title: Preparatory production of quercetin-3-β-D-glucopyranoside using alkali-tolerant thermostable α-L-rhamnosidase from Aspergillus terreus.
abstract: Extensive screening for a robust producer of α-L-rhamnosidase activity from well-defined strains of filamentous fungi, including multifactorial optimization (inducers, cultivation conditions) was accomplished. Enzyme production of the optimal producer Aspergillus terreus (non-toxigenic) was scaled up to 50L. α-L-Rhamnosidase, which was fully characterized, proved to be thermo- and alkali-tolerant, thus enabling effective operation at 70°C and pH 8.0. These conditions allow for a very high substrate (rutin) load up to 100-300 g/L, thus enabling very high volumetric productivity of the reaction product quercetin-3-β-D-glucopyranoside (isoquercitrin). Here, a novel concept of "immobilised substrate" is used. Isoquercitrin is a highly effective and biocompatible antioxidant with strong anti-inflammatory activities. Rutin biotransformation was optimized and scaled up to ca 10 kg production and thus the robustness of the large-scale production was demonstrated. Isoquercitrin can be produced to a very high purity (98%) in multikilogram amounts, without any quercetin and directly applicable in nutraceuticals.
authors: Institute of Microbiology, Center for Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors: Lenka
authors: L
authors: Weignerová
authors: Petr
authors: P
authors: Marhol
authors: Daniela
authors: D
authors: Gerstorferová
authors: Vladimír
authors: V
authors: Křen
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D000468:Alkalies / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D005960:Glucosides / Q000096:biosynthesis* / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D011794:Quercetin / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry; D012431:Rutin / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D000468:Alkalies; D005960:Glucosides; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C022525:alpha-L-rhamnosidase; C110309:quercetin 3-O-glucopyranoside