title: Detecting unknown sequences with DNA microarrays: explorative probe design strategies.
abstract: Designing environmental DNA microarrays that can be used to survey the extreme diversity of microorganisms existing in nature, represents a stimulating challenge in the field of molecular ecology. Indeed, recent efforts in metagenomics have produced a substantial amount of sequence information from various ecosystems, and will continue to accumulate large amounts of sequence data given the qualitative and quantitative improvements in the next-generation sequencing methods. It is now possible to take advantage of these data to develop comprehensive microarrays by using explorative probe design strategies. Such strategies anticipate genetic variations and thus are able to detect known and unknown sequences in environmental samples. In this review, we provide a detailed overview of the probe design strategies currently available to construct both phylogenetic and functional DNA microarrays, with emphasis on those permitting the selection of such explorative probes. Furthermore, exploration of complex environments requires particular attention on probe sensitivity and specificity criteria. Finally, these innovative probe design approaches require exploiting newly available high-density microarray formats.
authors: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France.
authors: Eric
authors: E
authors: Dugat-Bony
authors: Eric
authors: E
authors: Peyretaillade
authors: Nicolas
authors: N
authors: Parisot
authors: Corinne
authors: C
authors: Biderre-Petit
authors: Faouzi
authors: F
authors: Jaziri
authors: David
authors: D
authors: Hill
authors: Sébastien
authors: S
authors: Rimour
authors: Pierre
authors: P
authors: Peyret
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics; D001419:Bacteria / Q000145:classification / Q000235:genetics; D015342:DNA Probes; D005658:Fungi / Q000145:classification / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes