title: [Establishment and primary application of sandwich ELISA method to detect tenascin-C].
abstract: OBJECTIVE
  To establish a sandwich method to detect tenascin-c on the basis of preparation of monoclonal antibodies (mAbs) against tenascin-C (TN-C).
  
  
  METHODS
  The ascites of three stains of mAbs (No. 1A8, 3H7 and 4D6) were prepared and purified. The mAbs were conjugated with HRP and paired, respectively. The recombinant TN-C was taken as standard to analyze the optimal combination between mAbs. The sera TN-C concentrations of patients with osteosarocoma and the normal persons were evaluated with the sandwich ELISA method.
  
  
  RESULTS
  Among these mAbs, the sensitivity was obtained when combined the coated 1A8 with HRP-4D6. The sera TN-C significantly higher than the normal controls.
  
  
  CONCLUSIONS
  The sandwich ELISA method to detect TN-C was established successfully. The sera TN-C concentrations of patients with osteosarcoma and the normal persons were found distinct with the sandwich method.
authors: Department of Orthopaedics, Tangdu Hospital, Fourth Miliatry Medical University, Xi'an 710038, China.
authors: Yu-cai
authors: YC
authors: Wang
authors: Dian-Zhong
authors: DZ
authors: Zhang
authors: Li-hong
authors: LH
authors: He
authors: Yong
authors: Y
authors: Ding
authors: Le-qun
authors: LQ
authors: Shan
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012516:Osteosarcoma / Q000097:blood*; D019063:Tenascin / Q000097:blood*
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D019063:Tenascin