title: Large-scale plasmonic microarrays for label-free high-throughput screening.
abstract: Microarrays allowing simultaneous analysis of thousands of parameters can significantly accelerate screening of large libraries of pharmaceutical compounds and biomolecular interactions. For large-scale studies on diverse biomedical samples, reliable, label-free, and high-content microarrays are needed. In this work, using large-area plasmonic nanohole arrays, we demonstrate for the first time a large-scale label-free microarray technology with over one million sensors on a single microscope slide. A dual-color filter imaging method is introduced to dramatically increase the accuracy, reliability, and signal-to-noise ratio of the sensors in a highly multiplexed manner. We used our technology to quantitatively measure protein-protein interactions. Our platform, which is highly compatible with the current microarray scanning systems can enable a powerful screening technology and facilitate diagnosis and treatment of diseases.
authors: Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors: Tsung-Yao
authors: TY
authors: Chang
authors: Min
authors: M
authors: Huang
authors: Ahmet Ali
authors: AA
authors: Yanik
authors: Hsin-Yu
authors: HY
authors: Tsai
authors: Peng
authors: P
authors: Shi
authors: Serap
authors: S
authors: Aksu
authors: Mehmet Fatih
authors: MF
authors: Yanik
authors: Hatice
authors: H
authors: Altug
mesh_terms: D000818:Animals; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006041:Goats; D057166:High-Throughput Screening Assays / Q000379:methods*; D007074:Immunoglobulin G / Q000378:metabolism; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D059629:Signal-To-Noise Ratio; D020349:Surface Plasmon Resonance
chemical_list: D007074:Immunoglobulin G; D011506:Proteins; D016650:Fluorescein-5-isothiocyanate