title: Development of angiotensin II-induced abdominal aortic aneurysms is independent of catalase in mice.
abstract: Chronic infusion of angiotensin II (AngII) into mice augments the development of abdominal aortic aneurysms (AAAs). Catalase is an important antioxidant enzyme in cellular peroxisome, and it physiologically maintains tissue and cellular redox homeostasis and thus plays a central role in defense against oxidative stress. The purpose of this study was to define whether deficiency of catalase influences AngII-induced AAAs. Male acatalasemic (C3H/AnLCsCs) mice and wild-type (C3H/AnLCsCs) mice (8-12 weeks old, N = 24 and 25, respectively) were fed a normal chow for 5 weeks. After 1 week of acclimtion, mice were infused subcutaneously with AngII (1000 ng·kg·min) by osmotic minipumps for 4 weeks. AngII increased systolic blood pressure equivalently in both groups. Acatalasemia had no effect on serum cholesterol concentrations. The body weight of acatalasemic mice was slightly greater than that of wild-type mice (P = 0.008). Although aortic catalase activity in acatalasemic mice was significantly low (P < 0.001), acatalasemia had no significant effect on the incidence of AngII-induced AAA formation (acatalasemia, 23%; wild, 21%), ex vivo measurement of maximal diameter of abdominal aorta (acatalasemia, 1.22 ± 0.29 mm; wild, 1.21 ± 0.17 mm), or aortic deposition of lipid peroxidation products such as 4-hydroxy-2-nonenal. The development of AngII-induced AAAs is independent of catalase.
authors: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan. hauchida@md.okayama-u.ac.jp
authors: Haruhito A
authors: HA
authors: Uchida
authors: Hitoshi
authors: H
authors: Sugiyama
authors: Keiichi
authors: K
authors: Takiue
authors: Yoko
authors: Y
authors: Kikumoto
authors: Tatsuyuki
authors: T
authors: Inoue
authors: Hirofumi
authors: H
authors: Makino
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D000804:Angiotensin II / Q000633:toxicity*; D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000139:chemically induced* / Q000473:pathology; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D018345:Mice, Knockout; D018384:Oxidative Stress
chemical_list: D000447:Aldehydes; D000804:Angiotensin II; D002784:Cholesterol; D002374:Catalase; C027576:4-hydroxy-2-nonenal