title: 13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water.
abstract: NMR-based analysis of glycans by directly observing hydroxyl protons has been difficult because of their inherently fast exchange with water. We observed hydroxyl proton exchanges in a LewisX-LewisX interaction by using deuterium isotope shifts on (13)C-NMR. This strategy is suitable for analyzing weak interactions by identifying involved protons.
authors: Structural Glycobiology Team, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan.
authors: Shinya
authors: S
authors: Hanashima
authors: Koichi
authors: K
authors: Kato
authors: Yoshiki
authors: Y
authors: Yamaguchi
mesh_terms: D002247:Carbon Isotopes / Q000737:chemistry; D003903:Deuterium / Q000737:chemistry; D006860:Hydrogen Bonding; D016256:Lewis X Antigen / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry; D011522:Protons; D013696:Temperature; D014312:Trisaccharides / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D016256:Lewis X Antigen; D011134:Polysaccharides; D011522:Protons; D014312:Trisaccharides; C110530:galactosyl-(1,4)-fucopyranosyl-(1,3)-N-acetylglucosamine; D014867:Water; D003903:Deuterium