title: An immediate-early protein of white spot syndrome virus modulates the phosphorylation of focal adhesion kinase of shrimp.
abstract: WSSV interacts with integrin during infection of shrimps and modulate the focal adhesion kinase which is known as a regulator of several downstream signaling pathways. Viral protein kinases are thought to be important for virus infection by regulating the host signaling pathways. WSV083 is an immediate-early gene of white spot syndrome virus that contains a Ser/Thr protein kinase domain. So, does WSSV modulate FAK phosphorylation via the WSV083 molecule? In this study, co-transfection of WSV083 and MjFAK genes proceeded in insect cells revealed that the MjFAK phosphorylation and cell adhesion activity could be inhibited by the expression of WSV083. Kinase domain mutants of WSV083 lost its ability of inhibiting FAK phosphorylation. Moreover, silencing of FAK gene through RNAi accelerated the shrimp death rate upon WSSV challenge. These results demonstrate for the first time that modulation of FAK phosphorylation by WSV083 plays a critical role in the pathogenesis of WSSV infection.
authors: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors: Huasong
authors: H
authors: Lu
authors: Lingwei
authors: L
authors: Ruan
authors: Xun
authors: X
authors: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007313:Insecta; D016023:Integrins / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology* / Q000821:virology*; D010766:Phosphorylation; D034622:RNA Interference; D034741:RNA, Small Interfering; D015398:Signal Transduction; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D046848:White spot syndrome virus 1 / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D017874:Immediate-Early Proteins; D016023:Integrins; D034741:RNA, Small Interfering; D014764:Viral Proteins; D051416:Focal Adhesion Protein-Tyrosine Kinases