title: [Two single nucleotide polymorphisms on chromosome 3 and the risk of prostate cancer in Chinese men].
abstract: OBJECTIVE
  To investigate the correlation of the common variant single nucleotide polymorphisms (SNP) on chromosome 3 with the incidence and related risk factors of prostate cancer (PCa) in Chinese men.
  
  
  METHODS
  Using the case-control meth- od, we included 124 PCa patients in the PCa group and 111 age- and gender-matched cancer-free healthy subjects as normal controls. We detected the distribution of allele and genotype frequencies of the SNP rs10934853 and rs2660753 with the polymerase chain reaction-high resolution melting curve (PCR-HRM) combined with gene sequencing, analyzed the cumulative effect of the risk genotypes of these two independent variants, and determined the correlation between different genotypes of these two SNPs and clinically related risk factors in the PCa patients.
  
  
  RESULTS
  As for the genotypes of rs10934853, there were 28 cases of AA (22.8%), 46 cases of CC (37.4%), and 49 cases of AC (39.8%) in the PCa patients, as compared with 24 (22.0%), 34 (31.2%) and 51 (46.8%) in the healthy controls. As regards the genotypes of rs2660753, there were 13 cases of AA (11.0%), 59 cases of GG (50.0%) and 46 cases of AG (39.0%) in the PCa patients, in comparison with 9 (8.8%), 47 (45.6%) and 47 (45.6%) in the controls. No significant differences were found in the distribution of the genotype and allele frequencies of rs10934853 and rs2660753 between the two groups (P = 0.520 & 0.582). Analysis on the cumulative effect of the risk genotypes of rs10934853 and rs2660753 showed a slightly higher risk of PCa (OR = 1.831 & 1.968) in the two groups with risk genotypes than in the one with wild types (P > 0.05). Different genotypes of rs10934853 and rs2660753 were not correlated with clinically related risk factors of the PCa patients (P > 0.05).
  
  
  CONCLUSIONS
  SNP rs10934853 and rs2660753 on chromosome 3 are not obviously correlated with PCa in Chinese patients, and may not be a genetic risk factor of PCa.
authors: The Key Laboratory of Geratrics, Institute of Geriatrics, Beijing, 100730, China. zltsmc2002@163.com
authors: Lin
authors: L
authors: Zhou
authors: Ming
authors: M
authors: Liu
authors: Jin
authors: J
authors: Huang
authors: Yi-Ge
authors: YG
authors: Yang
authors: Dong
authors: D
authors: Wei
authors: Xiao-Hong
authors: XH
authors: Shi
authors: Yao-Guang
authors: YG
authors: Zhang
authors: Xin
authors: X
authors: Wang
authors: Chang-Hu
authors: CH
authors: Zhou
authors: Xin
authors: X
authors: Chen
authors: Ze
authors: Z
authors: Yang
authors: Jian-Ye
authors: JY
authors: Wang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002893:Chromosomes, Human, Pair 3; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011471:Prostatic Neoplasms / Q000453:epidemiology* / Q000235:genetics; D012307:Risk Factors