title: Anomalies in evaporative light scattering detection.
abstract: A two-dimensional (2-D) "heart-cutting" HPLC system was used to fractionate oligostyrenes into the respective diastereoisomers. For samples of known composition, the response of an ultraviolet (UV) absorbance detector followed the anticipated pattern. The response of an evaporative light-scattering (ELSD) detector on the other hand indicated quite different concentrations for the two diastereoisomers, relative to what was anticipated and what was indicated by the UV detector. Whereas approximately the same concentration was indicated by UV, ELSD in some cases indicated no detection of the later eluting isomer. The magnitude of the errors depended on both the molecular weight and the tacticity of the diastereomers. These anomalies appear to be an artifact of power transform functions imbedded within the firmware processor of the ELSD, invisible to the user.
authors: Australian Centre for Research On Separation Science (ACROSS), School of Natural Sciences, University of Western Sydney Parramatta Campus, Locked Bag 1797, South Penrith DC, South Penrith, NSW, 1797, Australia.
authors: D
authors: D
authors: Shock
authors: G R
authors: GR
authors: Dennis
authors: G
authors: G
authors: Guiochon
authors: P K
authors: PK
authors: Dasgupta
authors: R A
authors: RA
authors: Shalliker