title: 89Zr-nanocolloidal albumin-based PET/CT lymphoscintigraphy for sentinel node detection in head and neck cancer: preclinical results.
abstract: Identifying sentinel nodes near the primary tumor remains a problem in, for example, head and neck cancer because of the limited resolution of current lymphoscintigraphic imaging when using (99m)Tc-nanocolloidal albumin. This study describes the development and evaluation of a nanocolloidal albumin-based tracer specifically dedicated for high-resolution PET detection.
  
  
  METHODS
  (89)Zr was coupled to nanocolloidal albumin via the bifunctional chelate p-isothiocyanatobenzyldesferrioxamine B. Quality control tests, including particle size measurements, and in vivo biodistribution and imaging experiments in a rabbit lymphogenic metastasis model were performed.
  
  
  RESULTS
  Coupling of (89)Zr to nanocolloidal albumin appeared to be efficient, resulting in a stable product with a radiochemical purity greater than 95%, without affecting the particle size. PET showed distinguished uptake of (89)Zr-nanocolloidal albumin in the sentinel nodes, with visualization of lymphatic vessels, and with a biodistribution comparable to (99m)Tc-nanocolloidal albumin.
  
  
  CONCLUSIONS
  (89)Zr-nanocolloidal albumin is a promising tracer for sentinel node detection by PET.
authors: Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands.
authors: Derrek A
authors: DA
authors: Heuveling
authors: Gerard W M
authors: GW
authors: Visser
authors: Marian
authors: M
authors: Baclayon
authors: Wouter H
authors: WH
authors: Roos
authors: Gijs J L
authors: GJ
authors: Wuite
authors: Otto S
authors: OS
authors: Hoekstra
authors: C René
authors: CR
authors: Leemans
authors: Remco
authors: R
authors: de Bree
authors: Guus A M S
authors: GA
authors: van Dongen
mesh_terms: D000418:Albumins; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging / Q000556:secondary; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006258:Head and Neck Neoplasms / Q000000981:diagnostic imaging*; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging*; D010316:Particle Size; D049268:Positron-Emission Tomography; D011817:Rabbits; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D013668:Technetium Tc 99m Aggregated Albumin; D014057:Tomography, X-Ray Computed; D015040:Zirconium
chemical_list: D000418:Albumins; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D013668:Technetium Tc 99m Aggregated Albumin; D015040:Zirconium