title: Cloning of the herpes simplex virus type 1 genome as a novel luciferase-tagged infectious bacterial artificial chromosome.
abstract: Herpes simplex virus type 1 (HSV-1) is a ubiquitous human pathogen of skin and mucous membranes. In the present study, the genome of the HSV-1 F strain was cloned as an infectious bacterial artificial chromosome (BAC) clone without any deletions of the viral genes. Additionally, a firefly luciferase cassette was inserted to generate a novel luciferase-expressing HSV-1 BAC. Importantly, the resulting recombinant HSV-1 BAC Luc behaved indistinguishably from the wild-type virus in Vero cells, and the luciferase activity could be easily quantified in vitro. Thus, this novel HSV-1 BAC system would serve as a powerful tool for gene function profiling.
authors: Molecular Virology and Viral Immunology Research Group, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuchang, People's Republic of China.
authors: You
authors: Y
authors: Li
authors: Shuai
authors: S
authors: Wang
authors: Hua
authors: H
authors: Zhu
authors: Chunfu
authors: C
authors: Zheng
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D020869:Gene Expression Profiling; D016679:Genome, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D049409:Luciferases, Firefly / Q000235:genetics; D011995:Recombination, Genetic; D014162:Transfection; D014709:Vero Cells; D014774:Virulence / Q000235:genetics
chemical_list: D049409:Luciferases, Firefly