title: Phage lysin as a substitute for antibiotics to detect Mycobacterium tuberculosis from sputum samples with the BACTEC MGIT 960 system.
abstract: Phage lysin was evaluated as a substitute for antibiotics in sputum samples processed by a modified Petroff's method for the detection of Mycobacterium tuberculosis with the MGIT 960 system. One hundred and fifty sputum samples were processed, inoculated onto two slopes of Lowenstein-Jensen medium, and divided in to two aliquots of 0.5 mL each. One aliquot was added to 7 mL of MGIT medium containing polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin (PANTA) (MGIT-PANTA) and the other was added to 7 mL of MGIT medium containing 0.8 mL of lysin (MGIT-Lysin). The samples were randomized and incubated at 37°C in the MGIT 960 system. The sensitivity and specificity of MGIT-Lysin were 97% and 88%, respectively, as compared with MGIT-PANTA. The average times to detection with MGIT-Lysin and MGIT-PANTA were 9.3 and 8.6 days, respectively. The rate of contamination with MGIT-PANTA and MGIT-Lysin were 16% and 7.3%, respectively. Phage lysin can be substituted for antibiotics in processed sputum samples for the detection of M. tuberculosis.
authors: Department of Bacteriology, Tuberculosis Research Centre (Indian Council of Medical Research), Mayor V. R. Ramanathan Road,Chetput, Chennai, Tamil Nadu, India.
authors: B
authors: B
authors: Subramanyam
authors: G N
authors: GN
authors: Sivaramakrishnan
authors: A
authors: A
authors: Dusthackeer
authors: S
authors: S
authors: Nagamiah
authors: V
authors: V
authors: Kumar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000821:virology; D001431:Bacteriological Techniques; D001435:Bacteriophages / Q000201:enzymology*; D003470:Culture Media; D003666:Decontamination / Q000379:methods; D006801:Humans; D008242:Lysogeny; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media