title: A reappraisal of immunoglobulin variable gene primers and its impact on assessing clonal relationships between PB B cells and BM plasma cells in AL amyloidosis.
abstract: Monoclonal tumor plasma cells as well as non-terminally differentiated B cells having a clonal relationship to the tumor cells have been detected in the peripheral blood (PB) of some multiple myeloma (MM) patients but rarely in light chain (primary systemic) amyloidosis (AL) patients. Previously, our group found these peripheral clonotypic B cells in three AL patients. Here, we report detailed analysis of a larger cohort of AL patients to validate the prior findings and to investigate the effect of this cell population on clinical outcome. Fourteen AL patients were selected from a clinical prospective trial, and the relationship between immunoglobulin light chain variable gene (V(L)) representation in PB B cells and the clonal population in the bone marrow (BM) was investigated. A clonal relationship was not detected, and the present study provides important insights into the disparity with the earlier data, including clinical history of the patients and methodological analysis.
authors: Department of Biochemistry and Molecular Biology, Rochester, MN 55905, USA.
authors: Nagaaki
authors: N
authors: Katoh
authors: Tanya L
authors: TL
authors: Poshusta
authors: Michelle K
authors: MK
authors: Manske
authors: Angela
authors: A
authors: Dispenzieri
authors: Morie A
authors: MA
authors: Gertz
authors: Roshini S
authors: RS
authors: Abraham
authors: Marina
authors: M
authors: Ramirez-Alvarado
mesh_terms: D000686:Amyloidosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018941:Antigens, CD19 / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D001775:Blood Circulation / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D017326:Clinical Trials, Phase III as Topic; D002999:Clone Cells; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D038041:Lymphopoiesis; D009101:Multiple Myeloma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D010950:Plasma Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011446:Prospective Studies
chemical_list: D018941:Antigens, CD19; D017931:DNA Primers; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D004247:DNA