title: Filter-aided sample preparation with dimethyl labeling to identify and quantify milk fat globule membrane proteins.
abstract: Bovine milk is a major nutrient source in many countries and it is produced at an industrial scale. Milk is a complex mixture of proteins, fats, carbohydrates, vitamins and minerals. The composition of the bovine milk samples can vary depending on the genetic makeup of the bovine species as well as environmental factors. It is therefore important to study the qualitative and quantitative differences of bovine milk samples. Proteins in milk can be present in casein micelles, in the serum (the water soluble fraction) or in fat globules. These fat globules have a double membrane layer with proteins being bound to or being incapsulated in the membrane layer. The identification and molecular composition of the milk proteins have gained increased interest in recent years. Proteomic techniques make it now possible to identify up to many thousands of proteins in one sample, however quantification of proteins is as yet not straightforward. We analyzed the proteins of the milk fat globule membrane using dimethyl labeling methods combined with a filter-aided sample preparation protocol. Using these methods, it is now possible to quantitatively study the detailed protein composition of many milk samples in a short period of time.
authors: Dairy Science and Technology, Product Design and Quality Management Group, Wageningen University and Research Center, Bomenweg 2, 6700 EV, Wageningen, Netherlands.
authors: Jing
authors: J
authors: Lu
authors: S
authors: S
authors: Boeren
authors: S C
authors: SC
authors: de Vries
authors: H J F
authors: HJ
authors: van Valenberg
authors: J
authors: J
authors: Vervoort
authors: K
authors: K
authors: Hettinga
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D002417:Cattle; D005374:Filtration / Q000379:methods*; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D066292:Lipid Droplets; D008565:Membrane Proteins / Q000032:analysis*; D008697:Methane / Q000737:chemistry; D008894:Milk Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013997:Time Factors
chemical_list: D000890:Anti-Infective Agents; D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D008894:Milk Proteins; C110367:milk fat globule; D008697:Methane