title: The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations.
abstract: Microorganisms, such as Pseudomonas putida, utilize specific physical properties of cellular membrane constituents, mainly glycerophospholipids, to (re-)adjust the membrane barrier to environmental stresses. Building a basis for membrane composition/function studies, we inventoried the glycerophospholipids of different Pseudomonas and challenged membranes of growing cells with n-butanol. Using a new high-resolution liquid chromatography/mass spectrometry (LC/MS) method, 127 glycerophospholipid species [e.g. phosphatidylethanolamine PE(32:1)] with up to five fatty acid combinations were detected. The glycerophospholipid inventory consists of 305 distinct glycerophospholipids [e.g. PE(16:0/16:1)], thereof 14 lyso-glycerophospholipids, revealing conserved compositions within the four investigated pseudomonads P. putida KT2440, DOT-T1E, S12 and Pseudomonas sp. strain VLB120. Furthermore, we addressed the influence of environmental conditions on the glycerophospholipid composition of Pseudomonas via long-time exposure to the sublethal n-butanol concentration of 1% (v/v), focusing on: (i) relative amounts of glycerophospholipid species, (ii) glycerophospholipid head group composition, (iii) fatty acid chain length, (iv) degree of saturation and (v) cis/trans isomerization of unsaturated fatty acids. Observed alterations consist of changing head group compositions and for the solvent-sensitive strain KT2440 diminished fatty acid saturation degrees. Minor changes in the glycerophospholipid composition of the solvent-tolerant strains P. putida S12 and Pseudomonas sp. VLB120 suggest different strategies of the investigated Pseudomonas to maintain the barrier function of cellular membranes.
authors: Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, 44221 Dortmund, Germany.
authors: Jana
authors: J
authors: Rühl
authors: Eva-Maria
authors: EM
authors: Hein
authors: Heiko
authors: H
authors: Hayen
authors: Andreas
authors: A
authors: Schmid
authors: Lars M
authors: LM
authors: Blank
mesh_terms: D020001:1-Butanol / Q000378:metabolism; D005075:Biological Evolution; D053898:Biosynthetic Pathways; D002851:Chromatography, High Pressure Liquid; D020404:Glycerophospholipids / Q000096:biosynthesis* / Q000737:chemistry; D013058:Mass Spectrometry; D015394:Molecular Structure; D016958:Pseudomonas putida / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
chemical_list: D020404:Glycerophospholipids; D020001:1-Butanol