title: Evidence for osteocyte regulation of bone homeostasis through RANKL expression.
abstract: Osteocytes embedded in bone have been postulated to orchestrate bone homeostasis by regulating both bone-forming osteoblasts and bone-resorbing osteoclasts. We find here that purified osteocytes express a much higher amount of receptor activator of nuclear factor-κB ligand (RANKL) and have a greater capacity to support osteoclastogenesis in vitro than osteoblasts and bone marrow stromal cells. Furthermore, the severe osteopetrotic phenotype that we observe in mice lacking RANKL specifically in osteocytes indicates that osteocytes are the major source of RANKL in bone remodeling in vivo.
authors: Department of Cell Signaling, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Yushima, Bunkyo-ku, Tokyo, Japan.
authors: Tomoki
authors: T
authors: Nakashima
authors: Mikihito
authors: M
authors: Hayashi
authors: Takanobu
authors: T
authors: Fukunaga
authors: Kosaku
authors: K
authors: Kurata
authors: Masatsugu
authors: M
authors: Oh-Hora
authors: Jian Q
authors: JQ
authors: Feng
authors: Lynda F
authors: LF
authors: Bonewald
authors: Tatsuhiko
authors: T
authors: Kodama
authors: Anton
authors: A
authors: Wutz
authors: Erwin F
authors: EF
authors: Wagner
authors: Josef M
authors: JM
authors: Penninger
authors: Hiroshi
authors: H
authors: Takayanagi
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D005269:Femur / Q000166:cytology / Q000000981:diagnostic imaging; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D010010:Osteoclasts / Q000378:metabolism / Q000502:physiology; D010011:Osteocytes / Q000378:metabolism* / Q000502:physiology; D010022:Osteopetrosis / Q000378:metabolism*; D053245:RANK Ligand / Q000172:deficiency / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D055114:X-Ray Microtomography
chemical_list: D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins