title: Nuclear receptor mRNA expression by HBV in human hepatoblastoma cell lines.
abstract: Recent studies have implicated nuclear receptors (NRs) in the development of hepatocarcinogenesis. We assumed that hepatitis B virus (HBV) alters the expression of NRs and coregulators, and compared the gene expression profiling for 84 NRs and related genes between HpeG2.2.15, which secretes complete HBV virion, and HepG2 by real-time RT-PCR with SyBr green. Forty (47.6%) genes were upregulated 2-fold or greater, and only 5 (5.9%) were downregulated 2-fold or more, in HepG2.2.15 compared to HepG2. These results suggest that HBV affects NRs and their related signal transduction, and that they play important roles in viral replication and HBV-related hepatocarcinogenesis.
authors: Department of Medicine and Clinical Oncology, Chiba University, Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba 260-8677, Japan.
authors: Shuang
authors: S
authors: Wu
authors: Tatsuo
authors: T
authors: Kanda
authors: Fumio
authors: F
authors: Imazeki
authors: Shingo
authors: S
authors: Nakamoto
authors: Hiroshi
authors: H
authors: Shirasawa
authors: Osamu
authors: O
authors: Yokosuka
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D002472:Cell Transformation, Viral; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018197:Hepatoblastoma / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear