title: Polymorphism and parent-of-origin effects on gene expression of CAST, leptin and DGAT1 in cattle.
abstract: This study aimed to investigate differential allele expression (DAE) and polymorphism and parent-of-origin effects on expression of genes related to beef traits. CAST, related to meat tenderness, and DGAT1 and leptin, related to fat deposition, were evaluated. In bovine fetal tissues CAST was expressed twice as much (P < 0.05) in muscle of homozygous GG than in heterozygous AG. Leptin was expressed about one-tenth as much (P < 0.05) in heterozygous TpCm (allele T of paternal origin and allele C of maternal origin) than in homozygous CC. No DAE was observed. The evidence of polymorphism effect on expression of CAST and parent-of-origin effect on leptin contributes to a better understanding of events controlling the expression of genes of economic interest in cattle. Furthermore, if the parent-of-origin effects observed in fetal tissues are confirmed in adult tissues and associated to phenotypic variation, this parental origin criterion may be considered in marker-assisted selection of beef traits.
authors: Embrapa Cattle-Southeast, Rodovia Washington Luiz, km 234, CEP 13560-970, São Carlos, SP, Brazil.
authors: Simone Cristina Méo
authors: SC
authors: Niciura
authors: Adriana Mércia Guaratini
authors: AM
authors: Ibelli
authors: Gisele Veneroni
authors: GV
authors: Gouveia
authors: Juliana Gracielle Gonzaga
authors: JG
authors: Gromboni
authors: Marina Ibelli Pereira
authors: MI
authors: Rocha
authors: Marcela Maria
authors: MM
authors: de Souza
authors: Suelen Scarpa
authors: SS
authors: de Mello
authors: Luciana Correia de Almeida
authors: LC
authors: Regitano
mesh_terms: D000483:Alleles; D000818:Animals; D001823:Body Composition / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle / Q000235:genetics*; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006579:Heterozygote; D006720:Homozygote; D040582:Inheritance Patterns; D020738:Leptin / Q000235:genetics* / Q000378:metabolism; D008460:Meat; D020641:Polymorphism, Single Nucleotide
chemical_list: D002135:Calcium-Binding Proteins; D020738:Leptin; C033668:calpastatin; D051048:Diacylglycerol O-Acyltransferase