title: Mesenchymal stromal cells may enhance metastasis of neuroblastoma via SDF-1/CXCR4 and SDF-1/CXCR7 signaling.
abstract: Bone marrow metastasis is frequently observed in patients with high-risk neuroblastoma. Mesenchymal stromal cells (MSCs) in bone marrow may enhance tumor metastasis through secreting stromal cell-derived factor-1 (SDF-1). Here we investigated neuroblastoma cell behaviors under the influence of MSCs and explored the function of SDF-1 signaling during metastasis. Neuroblastoma expressed both of the SDF-1 receptors CXCR4 and CXCR7. shRNA knockdown showed that these receptors were responsible for the migration of neuroblastoma towards MSCs. CXCR4 also supported neuroblastoma invasion. These effects could be effectively blocked by AMD3100, a potent SDF-1 antagonist. Our study suggests that MSCs are important for neuroblastoma metastasis via the secretion of SDF-1 and that such effect can be inhibited by AMD3100 or shRNA knockdown.
authors: Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, PR China.
authors: Ming
authors: M
authors: Ma
authors: Jie Yu
authors: JY
authors: Ye
authors: Ruixia
authors: R
authors: Deng
authors: Cathleen Michelle
authors: CM
authors: Dee
authors: Godfrey Chi-Fung
authors: GC
authors: Chan
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D054377:Chemokine CXCL12 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis / Q000502:physiology; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D054387:Receptors, CXCR / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C501896:ACKR3 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D034741:RNA, Small Interfering; D054387:Receptors, CXCR; D019718:Receptors, CXCR4