title: An integrated enzyme-linked immunosorbent assay system with an organic light-emitting diode and a charge-coupled device for fluorescence detection.
abstract: A fluorescence detection system for a microfluidic device using an organic light-emitting diode (OLED) as the excitation light source and a charge-coupled device (CCD) as the photo detector was developed. The OLED was fabricated on a glass plate by photolithography and a vacuum deposition technique. The OLED produced a green luminescence with a peak emission at 512 nm and a half bandwidth of 55 nm. The maximum external quantum efficiency of the OLED was 7.2%. The emission intensity of the OLED at 10 mA/cm(2) was 13 μW (1.7 mW/cm(2)). The fluorescence detection system consisted of the OLED device, two band-pass filters, a five microchannel poly(dimethylsiloxane) (PDMS) microfluidic device and a linear CCD. The fluorescence detection system was successfully used in a flow-based enzyme-linked immunosorbent assay on a PDMS microfluidic device for the rapid determination of immunoglobulin A (IgA), a marker for human stress. The detection limit (S/N=3) for IgA was 16.5 ng/mL, and the sensitivity was sufficient for evaluating stress. Compared with the conventional 96-well microtiter plate assay, the analysis time and the amounts of reagent and sample solutions could all be reduced.
authors: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Hachioji, Tokyo, Japan. nakajima-hizuru@tmu.ac.jp
authors: Hizuru
authors: H
authors: Nakajima
authors: Yukiko
authors: Y
authors: Okuma
authors: Kazuhiro
authors: K
authors: Morioka
authors: Mayo
authors: M
authors: Miyake
authors: Akihide
authors: A
authors: Hemmi
authors: Tatsuya
authors: T
authors: Tobita
authors: Masayuki
authors: M
authors: Yahiro
authors: Daisuke
authors: D
authors: Yokoyama
authors: Chihaya
authors: C
authors: Adachi
authors: Nobuaki
authors: N
authors: Soh
authors: Koji
authors: K
authors: Nakano
authors: Shuhua
authors: S
authors: Xue
authors: Hulie
authors: H
authors: Zeng
authors: Katsumi
authors: K
authors: Uchiyama
authors: Toshihiko
authors: T
authors: Imato
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D007202:Indicators and Reagents; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*
chemical_list: D007070:Immunoglobulin A; D007202:Indicators and Reagents