title: Characteristics of lymphocyte nuclear factor-κB signal transduction kinase expression in aging process and regulatory effect of epimedium flavonoids.
abstract: OBJECTIVE
  To study the characteristics of lymphocyte nuclear factor kappa B (NF-κB) signal transduction kinase-related molecular mRNA differential expressions at various month age segments in aging process and the intervening effect of Epimedium flavonoids (EF) on it.
  
  
  METHODS
  Sixty SD rats were divided into six groups, according to animals' age, i.e., the 3 days (d) group, the 4 months (m) group, the 10 m group, the 18 m group, the 27 m group, and the 27 m+EF group. RNA was extracted from separated splenic lymphocytes. Adopting NF-κB signal path functional genome oligonucleotide gene-chip (128 related genes), the integral characteristics and differences of NF-κB signal transduction kinase-related mRNA expressions were determined, and the intervening effect of EF was examined.
  
  
  RESULTS
  The mean level of the NF-κB signal transduction kinase-related mRNA expressions in rats' splenic lymphocytes lowered with aging; the highest expression was presented at 3 d after birth, and then, it lowered gradually, with the lowest level at 18 m or 27 m. After EF intervention, the expression level was raised to the 10-18 m level in the aged rats.
  
  
  CONCLUSIONS
  The changing rules of lymphocyte NF-κB-signal-transduction-kinase-related mRNA expressions in various stages of aging are helpful for selecting the well time for preventing and intervening aging, and will also give a hint to the molecular index for assessment of senility retarding researches.
authors: Shanghai Traditional Chinese Medicine Clinical Center of Cardio-cerebrovascular Diseases in Tenth People's Hospital of Tongji University, China.
authors: Xiao-yu
authors: XY
authors: Liu
authors: Qi
authors: Q
authors: Wang
authors: Shi-jin
authors: SJ
authors: Xia
authors: Jian-hua
authors: JH
authors: Huang
authors: Zi-yin
authors: ZY
authors: Shen
authors: Hao
authors: H
authors: Xu
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D034181:Epimedium / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D051550:I-kappa B Kinase / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D000097782:NF-kappaB-Inducing Kinase
chemical_list: D005419:Flavonoids; D051550:I-kappa B Kinase; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000097782:NF-kappaB-Inducing Kinase