title: [Combination of AD5-10 and epirubicin in treating rheumatoid arthritis].
abstract: OBJECTIVE
  To investigate the mechanism of anti-death receptor 5-10 (AD5-10) combined with epirubicin in treating rheumatoid arthritis (RA).
  
  
  METHODS
  We detected the cell viability of the fibroblast-like synoviocytes (FLS) from RA patients with MTT. The expression level of apoptosis signaling pathways protein, p53, and p21 were evaluated with Western blot.
  
  
  RESULTS
  We found that epirubicin, at different doses, could enhance the effect of AD5-10 on FLS, promoting the apoptosis of FLS. The expression levels of caspase-3, -8, -9, c-FLIP, Bcl-2, p53, and p21 in the FLS changed after epirubicin treatment.
  
  
  CONCLUSIONS
  Epirubicin may coordinate with AD5-10 in inducing FLS apoptosis through affecting the levels of p53, p21, c-FLIP, and Bcl-2.
authors: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Beijing, China.
authors: Jian-suo
authors: JS
authors: Zhou
authors: Juan
authors: J
authors: Shi
authors: Jie-qing
authors: JQ
authors: Zhu
authors: Hai-qin
authors: HQ
authors: Yuan
authors: Yan-xin
authors: YX
authors: Liu
authors: Xin
authors: X
authors: You
authors: De-xian
authors: DX
authors: Zheng
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D015251:Epirubicin / Q000494:pharmacology*; D006801:Humans; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000276:immunology*; D013583:Synovial Membrane / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D051017:Apoptosis Regulatory Proteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D015251:Epirubicin