title: Pretreatment with an ethanolic extract of Taiwanofungus camphoratus (Antrodia camphorata) enhances the cytotoxic effects of amphotericin B.
abstract: Taiwanofungus camphoratus, a well-known Chinese medicine used in Taiwan, possesses several pharmacological functions, including anticancer effects. In the present study, we aimed to investigate a novel anticancer effect by pretreating cancer cells with an ethanolic extract of T. camphoratus (TCEE) followed by the administration of an antifungal agent amphotericin B (AmB). Both TCEE and AmB showed significant dose-dependent cytotoxicity in HT29 cells. Pretreatment with a nontoxic dose of TCEE enhanced the cytotoxicity of AmB. Furthermore, significant apoptotic cell death was found in cells treated with TCEE and AmB. A combination treatment with AmB plus TCEE resulted in a significant repression of tumor growth in HT29 xenografts. Collectively, our results indicated that combined treatment with AmB and TCEE effectively induced apoptosis and inhibited tumor growth. In the future, TCEE may serve as a potential complementary and alternative medicine to treat patients suffering from colorectal cancer.
authors: Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan, Republic of China.
authors: Ling-Yi
authors: LY
authors: Chen
authors: Ming-Thau
authors: MT
authors: Sheu
authors: Der-Zen
authors: DZ
authors: Liu
authors: Chuh-Kai
authors: CK
authors: Liao
authors: Hsiu-O
authors: HO
authors: Ho
authors: Woei-Yao
authors: WY
authors: Kao
authors: Yaun-Soon
authors: YS
authors: Ho
authors: Wen-Sen
authors: WS
authors: Lee
authors: Ching-Hua
authors: CH
authors: Su
mesh_terms: D000666:Amphotericin B / Q000008:administration & dosage*; D000818:Animals; D000935:Antifungal Agents; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000302:isolation & purification; D000971:Antineoplastic Combined Chemotherapy Protocols; D055491:Antrodia / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology; D000431:Ethanol; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D013624:Taiwan; D014315:Triterpenes / Q000008:administration & dosage / Q000032:analysis
chemical_list: D000935:Antifungal Agents; D000970:Antineoplastic Agents; D014315:Triterpenes; D000431:Ethanol; D000666:Amphotericin B