title: A micropatterning and image processing approach to simplify measurement of cellular traction forces.
abstract: Quantification of the traction forces that cells apply to their surroundings has been critical to the advancement of our understanding of cancer, development and basic cell biology. This field was made possible through the development of engineered cell culture systems that permit optical measurement of cell-mediated displacements and computational algorithms that allow conversion of these displacements into stresses and forces. Here, we present a novel advancement of traction force microscopy on polyacrylamide (PAA) gels that addresses limitations of existing technologies. Through an indirect patterning technique, we generated PAA gels with fluorescent 1 μm dot markers in a regularized array. This improves existing traction measurements since (i) multiple fields of view can be measured in one experiment without the need for cell removal; (ii) traction vectors are modeled as discrete point forces, and not as a continuous field, using an extremely simple computational algorithm that we have made available online; and (iii) the pattern transfer technique is amenable to any of the published techniques for producing patterns on glass. In the future, this technique will be used for measuring traction forces on complex patterns with multiple, spatially distinct ligands in systems for applying strain to the substrate, and in sandwich cultures that generate quasi-three-dimensional environments for cells.
authors: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.
authors: Samuel R
authors: SR
authors: Polio
authors: Katheryn E
authors: KE
authors: Rothenberg
authors: Dimitrije
authors: D
authors: Stamenović
authors: Michael L
authors: ML
authors: Smith
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000465:Algorithms; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001696:Biomechanical Phenomena; D002448:Cell Adhesion; D002465:Cell Movement; D005353:Fibronectins / Q000737:chemistry; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods; D041681:NIH 3T3 Cells; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D005353:Fibronectins; C016680:polyacrylamide gels