title: Dietary long-chain n-3 PUFA, gut microbiota and fat mass in early postnatal piglet development--exploring a potential interplay.
abstract: Dietary n-3PUFA and gut bacteria, particularly Bacteroidetes, have been suggested to be related to adiposity. We investigated if n-3PUFA affected fat storage and cecal bacteria in piglets. Twenty-four 4-day-old piglets were allocated to formula rich in n-3PUFA (∼3E%) from fish oil (FO) or n-6PUFA from sunflower oil (SO) for 14 days. We assessed body weight, fat accumulation by dual-energy X-ray absorptiometry and microbial molecular fingerprints. Dietary PUFA-composition was reflected in higher erythrocyte n-3PUFA in the FO- than the SO-group (P<0.001). Principal component analysis revealed group differences in the overall microbiotic composition, which involved a larger Bacteroides community in the SO-group (P=0.02). There was no significant difference in body fat percentage and no relationship between fat accumulation and gut Bacteroides. Hence, this study does not support an impact of n-3PUFA or microbiota on fat accumulation during the postnatal maturation period. The impact of dietary PUFA on the gut Bacteroides warrants further investigation.
authors: Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
authors: A D
authors: AD
authors: Andersen
authors: L
authors: L
authors: Mølbak
authors: T
authors: T
authors: Thymann
authors: K F
authors: KF
authors: Michaelsen
authors: L
authors: L
authors: Lauritzen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001439:Bacteroides; D001823:Body Composition; D001835:Body Weight; D002432:Cecum / Q000382:microbiology*; D004041:Dietary Fats / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005395:Fish Oils / Q000378:metabolism; D005526:Food, Formulated; D054892:Metagenome; D010938:Plant Oils / Q000378:metabolism; D025341:Principal Component Analysis; D000074242:Sunflower Oil; D034421:Sus scrofa / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D010938:Plant Oils; D000074242:Sunflower Oil