title: Molecular characterization and epidemiological investigation of Pigeon circovirus isolated in eastern China.
abstract: To investigate the prevalence and genetic characterization of Pigeon circovirus (PiCV) circulating in Chinese flocks, the genomic DNA of 144 samples collected from pigeons in 6 different geographic regions of eastern China between 2009 and 2010 were amplified using previously published PiCV primers. The PiCV sequence was detected in 83 of 104 unhealthy pigeons (79.8%) and 25 of 40 healthy pigeons (62.5%). The overall positive rate was 75% for all samples. An inverse primer polymerase chain reaction (IP-PCR) assay was performed to amplify the full-length sequence from a random sample of each region, and 6 specific DNA fragments were gel-purified and sequenced. The 6 full-length sequences were designated as SHWH-AB4 (2,031 bp), NJPK-21 (2,035 bp), HBLF-E2 (2,031 bp), JSJN (2,039 bp), SDDZ (2,037 bp), and AHBZ (2,035 bp) after BLAST analyses. The phylogenic tree and amino acid comparison indicated that all the strains examined were derived from a common strain, but had undergone genetic mutations through time. Pairwise comparisons revealed 93.4%-100% amino acid identity for the putative replication-associated proteins and 67.5%-100% for the putative capsid proteins.
authors: Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China.
authors: Zhicheng
authors: Z
authors: Zhang
authors: Chengping
authors: C
authors: Lu
authors: Yang
authors: Y
authors: Wang
authors: Shaohui
authors: S
authors: Wang
authors: Dingzhen
authors: D
authors: Dai
authors: Zhongming
authors: Z
authors: Chen
authors: Hongjie
authors: H
authors: Fan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology / Q000821:virology*; D002681:China / Q000453:epidemiology; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics*; D010856:Columbidae; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins