title: Genome-wide maps of histone modifications unwind in vivo chromatin states of the hair follicle lineage.
abstract: Using mouse skin, where bountiful reservoirs of synchronized hair follicle stem cells (HF-SCs) fuel cycles of regeneration, we explore how adult SCs remodel chromatin in response to activating cues. By profiling global mRNA and chromatin changes in quiescent and activated HF-SCs and their committed, transit-amplifying (TA) progeny, we show that polycomb-group (PcG)-mediated H3K27-trimethylation features prominently in HF-lineage progression by mechanisms distinct from embryonic-SCs. In HF-SCs, PcG represses nonskin lineages and HF differentiation. In TA progeny, nonskin regulators remain PcG-repressed, HF-SC regulators acquire H3K27me3-marks, and HF-lineage regulators lose them. Interestingly, genes poised in embryonic stem cells, active in HF-SCs, and PcG-repressed in TA progeny encode not only key transcription factors, but also signaling regulators. We document their importance in balancing HF-SC quiescence, underscoring the power of chromatin mapping in dissecting SC behavior. Our findings explain how HF-SCs cycle through quiescent and activated states without losing stemness and define roles for PcG-mediated repression in governing a fate switch irreversibly.
authors: Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology & Development, The Rockefeller University, New York, NY 10065, USA.
authors: Wen-Hui
authors: WH
authors: Lien
authors: Xingyi
authors: X
authors: Guo
authors: Lisa
authors: L
authors: Polak
authors: Lee N
authors: LN
authors: Lawton
authors: Richard A
authors: RA
authors: Young
authors: Deyou
authors: D
authors: Zheng
authors: Elaine
authors: E
authors: Fuchs
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D042002:Chromatin Assembly and Disassembly; D055106:Genome-Wide Association Study; D048091:Guided Tissue Regeneration; D018859:Hair Follicle / Q000378:metabolism* / Q000473:pathology; D006657:Histones / Q000378:metabolism*; D051379:Mice; D063146:Polycomb-Group Proteins; D011499:Protein Processing, Post-Translational; D012097:Repressor Proteins / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D012867:Skin / Q000473:pathology
chemical_list: D006657:Histones; D063146:Polycomb-Group Proteins; D012097:Repressor Proteins