title: Inhibitory effect of 2-arylbenzofurans from the Mori Cortex Radicis (Moraceae) on oxygen glucose deprivation (OGD)-induced cell death of SH-SY5Y cells.
abstract: Three known 2-arylbenzofurans, moracin P (1), moracin O (2) and mulberrofuran Q (3) were isolated from the MeOH extract of the Mori Cortex Radicis. These compounds 1-3 enhanced cell viability in dose-dependent manner against oxygen-glucose deprivation (OGD)-induced cell death in neuroblastoma SH-SY5Y cells, which was measured by MTT reduction assay. (EC(50) values of 10.4, 12.6, and 15.9 μM, respectively). In addition, the compounds 1-3 were examined for their inhibitory effect on OGD-induced ROS production by FACS analysis. We observed these compounds reduced ROS production in OGD-induced cell death (IC(50) values of 1.9, 0.3 and 12.1 μM, respectively). Consequently, reactive oxygen species (ROS) were overexpressed in OGD-induced cells and all three compounds reduced ROS induced by OGD in dosedependent manner. Taken together, compounds 1-3 might protect neuronal cell death against the oxidative stress induced by OGD, though further studies in vitro and in vivo models are necessary.
authors: Korea Forest Research Institute, Seoul 130-712, Korea.
authors: Hak Ju
authors: HJ
authors: Lee
authors: Da Hyun
authors: da H
authors: Lyu
authors: Uk
authors: U
authors: Koo
authors: Sung-Jin
authors: SJ
authors: Lee
authors: Seong Su
authors: SS
authors: Hong
authors: Kemok
authors: K
authors: Kim
authors: Kyeong Ho
authors: KH
authors: Kim
authors: Dongho
authors: D
authors: Lee
authors: Woongchon
authors: W
authors: Mar
mesh_terms: D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005562:Formazans / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D020128:Inhibitory Concentration 50; D029586:Moraceae; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D001572:Benzofurans; D005562:Formazans; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; C540980:moracin P; C079782:MTT formazan; D005947:Glucose; D010100:Oxygen