title: In planta expression of a mature Der p 1 allergen isolated from an Italian strain of Dermatophagoides pteronyssinus.
abstract: European (Dermatophagoides pteronyssinus) and American (Dermatophagoides farinae) house dust mite species are considered the most common causes of asthma and allergic symptoms worldwide. Der p 1 protein, one of the main allergens of D. pteronyssinus, is found in high concentration in mites faecal pellets, which can became easily airborne and, when inhaled, can cause perennial rhinitis and bronchial asthma. Here we report the isolation of the Der p 1 gene from an Italian strain of D. pteronyssinus and the PVX-mediated expression of its mature form (I-rDer p 1) in Nicotiana benthamiana plants. Human sera from characterized allergic patients were used for IgE binding inhibition assays to test the immunological reactivity of I-rDer p 1 produced in N. benthamiana plants. The binding properties of in planta produced I-rDer p 1 versus the IgE of patients sera were comparable to those obtained on Der p 1 preparation immobilized on a microarray. In this paper we provide a proof of concept for the production of an immunologically active form of Der p 1 using a plant viral vector. These results pave the way for the development of diagnostic allergy tests based on in planta produced allergens.
authors: Dipartimento di Biologia Applicata, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
authors: Gianpiero
authors: G
authors: Marconi
authors: Emidio
authors: E
authors: Albertini
authors: Adriano
authors: A
authors: Mari
authors: Paola
authors: P
authors: Palazzo
authors: Andrea
authors: A
authors: Porceddu
authors: Lorenzo
authors: L
authors: Raggi
authors: Luigi
authors: L
authors: Bolis
authors: Hovirag
authors: H
authors: Lancioni
authors: Antonella
authors: A
authors: Palomba
authors: Livia
authors: L
authors: Lucentini
authors: Luisa
authors: L
authors: Lanfaloni
authors: Francesco
authors: F
authors: Marcucci
authors: Mario
authors: M
authors: Falcinelli
authors: Fausto
authors: F
authors: Panara
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D060829:Arthropod Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D040001:Dermatophagoides farinae / Q000276:immunology; D040002:Dermatophagoides pteronyssinus / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces; D006031:Glycosylation; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D047468:Immunoprecipitation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012220:Rhinitis / Q000097:blood / Q000276:immunology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451159:Dermatophagoides farinae antigen f 1; C451162:Dermatophagoides pteronyssinus antigen p 1