title: Analysis of cellular migration using a two-chamber methodology.
abstract: Directed cell migration is fundamental to both physiological and pathophysiological processes such as embryogenesis, wound healing, and cancer metastasis. A complex series of events are required for directional cell migration, which is initiated by a migration-promoting or chemotactic stimulus, resulting in cellular polarization and entry into a cyclical pattern of leading edge protrusion, adhesion, and retraction of the trailing edge allowing cell movement. Heat shock proteins such as Hsp27, Hsp90, alphaB-crystallin, as well as heat shock transcription factors, are important players in both physiological and pathophysiological cell migration. A variety of techniques are currently available to assess cell migration, and among the most commonly utilized are those that employ a two-chamber methodology, such as that developed by Stephen Boyden in the early 1960s. Herein, we describe the use of a multiwell microchemotaxis migration assay that has the advantages of being reusable, inexpensive, highly reproducible, and requiring only a small volume of reagents.
authors: Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, VIC, Australia.
authors: Michelle M
authors: MM
authors: Kouspou
authors: John T
authors: JT
authors: Price
mesh_terms: D045744:Cell Line, Tumor; D054443:Cell Migration Assays / Q000295:instrumentation / Q000379:methods*; D002633:Chemotaxis; D003459:Crystallins / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D009362:Neoplasm Metastasis; D014157:Transcription Factors / Q000378:metabolism; D014945:Wound Healing
chemical_list: D003459:Crystallins; D055551:HSP27 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D014157:Transcription Factors