title: Spectral contrast imaging microscopy.
abstract: We introduce a new technique, spectral contrast imaging microscopy (SCIM), for super-resolution microscopic imaging. Based on a novel contrast mechanism that encodes each local spatial frequency with a corresponding optical wavelength, SCIM provides a real-time high-resolution spectral contrast microscopic image with superior contrast. We show that two microscopic objects, separated by a distance smaller than the diffraction limit of the optical system, can be spatially resolved in the SCIM image as different colors. Results with numerical simulation and experiments using a high-resolution United States Air Force target are presented. The ability of SCIM for imaging biological cells is also demonstrated.
authors: Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15232, USA. sea47@pitt.edu
authors: Sergey A
authors: SA
authors: Alexandrov
authors: Shikhar
authors: S
authors: Uttam
authors: Rajan K
authors: RK
authors: Bista
authors: Yang
authors: Y
authors: Liu
mesh_terms: D005583:Fourier Analysis; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging; D013997:Time Factors