title: Detection and characterization of aparmycin-resistant Escherichia coli from humans in Korea.
abstract: To investigate apramycin resistance in humans in Korea, a total of 138 human Escherichia coli strains confirmed as gentamicin-resistant were collected from Korean Culture Collection Antimicrobial-Resistant Microbes. Apramycin resistance (minimum inhibitory concentrations ≥1,024 μg/ml) was observed in 16 (11.6%) of the 138 gentamicin-resistant E. coli (GREC) strains. Among the seven different kinds of aminoglycoside resistance genes tested, only four kinds were detected in the apramycin-resistant GREC strains: aac (3)-II, aac (3)-III, aac (3)-IV, and armA. The aac (3)-IV gene was found in all apramycin-resistant GREC strains, whereas aac(3)-II, aac(3)-III, and armA genes were detected in 8 (50.0%), 6 (37.5%), and 1 (6.3%) GREC strains resistant to apramycin, respectively. Of 16 apramycin-resistant GREC strains, transfer of apramycin resistance was observed in seven (43.8%), and co-transfer of resistance to other antimicrobials along with apramycin resistance was also found in four strains (25.0%) by broth mating. The results of this study suggest that more prudential use of apramycin in animals is needed.
authors: National Veterinary Research and Quarantine Service, Anyang, Republic of Korea. imsk0049@korea.kr
authors: Suk-Kyung
authors: SK
authors: Lim
authors: Hyang-Mi
authors: HM
authors: Nam
authors: Aeran
authors: A
authors: Kim
authors: Gum-Chan
authors: GC
authors: Jang
authors: Hee-Soo
authors: HS
authors: Lee
authors: Suk-Chan
authors: SC
authors: Jung
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D016133:Polymerase Chain Reaction
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005839:Gentamicins; D009328:Nebramycin; C011666:apramycin