title: Identification of ionotrophic purinergic receptors in Huh-7 cells and their response towards structural proteins of HCV genotype 3a.
abstract: Hepatitis C virus (HCV) is a major health problem in developing countries including Pakistan. Chronic HCV infection results in progressive liver disease including fibrosis, cirrhosis, insulin resistance and eventually hepatocellular carcinoma (HCC). Ionotrophic purinergic (P2X) receptors are identified to involve in a spectrum of physiological and pathophysiological processes. However, the role of P2X receptors in HCV liver associated diseases still remains to be investigated. The current study was designed to identify the presence of P2X receptors in human liver cells. Furthermore, it investigates the response of P2X receptors towards HCV structural proteins (E1E2). To determine that how many isoforms of P2X receptors are expressed in human liver cells, human hepatoma cell line (Huh-7) was used. Transcripts (mRNA) of five different isoforms of P2X receptors were identified in Huh-7 cells. To examine the gene expression of identified isoforms of P2X receptors in presence of HCV structural proteins E1E2, Huh-7/E1E2 cell line (stably expressing HCV structural proteins E1E2) was used. The results showed significant increase (6.2 fold) in gene expression of P2X4 receptors in Huh-7/E1E2 cells as compared to control Huh-7 cells. The findings of present study confirmed the presence of transcripts of five different isoforms of P2X receptors in human liver cells and suggest that P2X4 receptors could be represented an important component of the purinergic signaling complex in HCV induced liver pathogenesis.
authors: National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
authors: Sobia
authors: S
authors: Manzoor
authors: Muhammad
authors: M
authors: Idrees
authors: Javed
authors: J
authors: Ashraf
authors: Azra
authors: A
authors: Mehmood
authors: Sadia
authors: S
authors: Butt
authors: Kaneez
authors: K
authors: Fatima
authors: Haji
authors: H
authors: Akbar
authors: Irshad U
authors: IU
authors: Rehaman
authors: Ishtiaq
authors: I
authors: Qadri
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D015870:Gene Expression; D005838:Genotype; D016174:Hepacivirus / Q000737:chemistry* / Q000235:genetics; D006526:Hepatitis C / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D008103:Liver Cirrhosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D010957:Plasmids; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D058848:Receptors, Purinergic P2X4 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C087035:E1 protein, Hepatitis C virus; D020033:Protein Isoforms; D012333:RNA, Messenger; D058848:Receptors, Purinergic P2X4; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus