title: [Effect of triiodothyronine on the bone proteins expression during osteogenic differentiation of mesenchymal stem cells].
abstract: OBJECTIVE
  The aim of this study was to evaluate the effect of T3 on the expression of osteocalcin, osteopontin and collagen I during osteogenic differentiation of mesenchymal stem cells (MSC).
  
  
  METHODS
  The bone marrow cells of Wistar rats with 30 days of age were extracted, cultured and separated into five groups: control (undifferentiated), differentiated (osteogenic stimulus) and differentiated with T3 (10(-3) nM, 10(-2) nM and 100 nM). For each group, four samples were cultured and were analyzed by real time RT-PCR at 7, 14 and 21 days for quantification of gene transcripts for osteocalcin, osteopontin and collagen I.
  
  
  RESULTS
  All the different groups without T3 or with T3 regardless of the concentration, showed the collagen I expression significantly lower expression, and osteocalcin and osteopontin expression significantly greater than that of undifferentiated MSC. Nevertheless, the group T3 100 nM showed higher expression of osteocalcin and a similar expression of the osteoblast culture.
  
  
  CONCLUSIONS
  In conclusion, the triiodothyronine does not affect the expression of osteopontin and collagen I, but increases ostecalcin expression during osteogenic differentiation in vitro of the MSC, and this effect is dose-dependent.
authors: Núcleo de Células-tronco, Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors: Rafaela C R
authors: RC
authors: Hell
authors: Jankerle N
authors: JN
authors: Boeloni
authors: Natália M
authors: NM
authors: Ocarino
authors: Juneo F
authors: JF
authors: Silva
authors: Alfredo M
authors: AM
authors: Goes
authors: Rogéria
authors: R
authors: Serakides
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D001857:Bone Matrix / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D011506:Proteins; D014284:Triiodothyronine; D015675:Osteocalcin; D053495:Osteopontin; D003094:Collagen