title: Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs.
abstract: Two experiments were conducted to evaluate effects of keratinase for growing and nursery pigs. In Exp. 1, six pigs (32.3 +/- 2.8 kg body weight), fitted with a simple T-cannula at the distal ileum, were assigned to one of two 3 x 3 Latin squares involving three periods and three diets including a basal diet and the same diets supplemented with 0, 0.05 or 0.1% keratinase. Dietary keratinase supplementation increased the apparent ileal digestibility of crude protein (CP), arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, alanine, glutamic acid and proline (p < 0.05). Digestibility coefficients did not differ between pigs fed 0.05 and 0.1% keratinase. In Exp. 2, 24 piglets weaned at 30 +/- 2 d of age were used in a 2 x 2 factorial design experiment with two CP concentrations (19 vs. 22%) and two levels of keratinase supplementation (0 vs. 0.05%). Keratinase supplementation increased (p < 0.05) average daily gain, serum arginine concentration and loin muscle area but decreased (p < 0.05) serum interleukin-10 concentrations. The reduction in dietary CP level decreased (p < 0.05) serum urea nitrogen concentrations, isoleucine, serine and proline concentrations, but increased serum arginine concentrations. Few interactions between keratinase supplementation and dietary CP concentration were observed. This study indicated that dietary keratinase supplementation improved apparent ileal amino acid digestibility for growing pigs and had a positive effect on weight gain, immune response and loin muscle area for nursery pigs.
authors: Ministry of Agriculture Feed Industry Centre, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.
authors: Ding
authors: D
authors: Wang
authors: Zhikai
authors: Z
authors: Zeng
authors: Xiangshu
authors: X
authors: Piao
authors: Pengfei
authors: P
authors: Li
authors: Lingfeng
authors: L
authors: Xue
authors: Qian
authors: Q
authors: Zhang
authors: Xu
authors: X
authors: Han
authors: Hongyu
authors: H
authors: Zhang
authors: Bing
authors: B
authors: Dong
authors: Sung Woo
authors: SW
authors: Kim
mesh_terms: D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D016207:Cytokines / Q000097:blood; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D005766:Gastrointestinal Contents / Q000737:chemistry; D007082:Ileum / Q000378:metabolism*; D007136:Immunoglobulins / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects / Q000254:growth & development; D010447:Peptide Hydrolases / Q000378:metabolism / Q000494:pharmacology*; D013025:Glycine max; D013552:Swine / Q000254:growth & development*; D015430:Weight Gain / Q000187:drug effects; D003313:Zea mays
chemical_list: D000596:Amino Acids; D016207:Cytokines; D007136:Immunoglobulins; D010447:Peptide Hydrolases; C035855:keratinase