title: Simultaneous determination of five synthetic pyrethroid metabolites in urine by liquid chromatography-tandem mass spectrometry: application to 39 persons without known exposure to pyrethroids.
abstract: A sensitive and reliable method was developed and validated for the determination of five synthetic pyrethroid metabolites namely cis-Cl(2)CA, trans-Cl(2)CA, Br(2)CA, 3-PBA and 4-FPBA in human urine by liquid chromatography-tandem mass spectrometry. (2)D(6)-labelled trans-Cl(2)CA and (13)C(6)-labelled 3-PBA were used as internal standards. This method was based on a liquid-liquid extraction procedure in acidic conditions using hexane solvent with a basic purification, a chromatographic separation using a specific C18 column and mass spectrometric detection in the negative polarity. Suitable limits of detection (0.015μg/L for the five compounds) and quantification (from 0.020 to 0.030μg/L) were obtained for rendering the method usable for the biomonitoring of pyrethroids in the general population. The efficiency of the method was tested in 39 urine samples from French people without any known exposure to pyrethroids. At least three of the five metabolites were detected in each sample. The results of this study were compared to those obtained in previous ones and discussed.
authors: Department of Pharmacology and Toxicology, University Hospital, 2 Avenue Martin Luther King, 87042 Limoges, France.
authors: R
authors: R
authors: Le Grand
authors: S
authors: S
authors: Dulaurent
authors: J M
authors: JM
authors: Gaulier
authors: F
authors: F
authors: Saint-Marcoux
authors: C
authors: C
authors: Moesch
authors: G
authors: G
authors: Lachâtre
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D004781:Environmental Exposure; D004784:Environmental Monitoring / Q000379:methods; D004785:Environmental Pollutants; D005260:Female; D006801:Humans; D007306:Insecticides / Q000737:chemistry* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D011722:Pyrethrins / Q000737:chemistry* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
chemical_list: D004785:Environmental Pollutants; D007306:Insecticides; D011722:Pyrethrins