title: Induction of Sca-1 in the duct cells of the mouse submandibular gland by obstruction of the main excretory duct.
abstract: The effect of ligation of the main excretory duct (MED) of the mouse submandibular gland (SMG) on the expression of Sca-1, a stem cell antigen, was examined by Western blotting and immunohistochemistry. By Western blotting, the expression of Sca-1 with a molecular weight of 18 kDa was identified in the normal gland. At 1 day post-ligation, the expression level of Sca-1 was strongly increased in the experimental gland and weakly in the contralateral gland, and such expression in both glands decreased at 6 days. By immunohistochemistry, Sca-1 was detected weakly in the apical membrane of excretory duct (ED) cells of the SMG under the normal condition. By duct ligation, Sca-1 became expressed strongly in most cells of the two major duct systems, i.e., the striated duct (SD) and granular convoluted tubules (GCT), but was not detected in the acinar (Ac) cells. By fluorescence-activated cell sorter (FACS) analysis, the number of side population (SP) cells in this gland was found to be increased by ligation. These results imply that Sca-1-positive cells may have a role in the duct cell proliferation in the regeneration step elicited by MED ligation-induced injury.
authors: Department of Molecular Oral Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima-shi, Tokushima, Japan.
authors: Nunuk
authors: N
authors: Purwanti
authors: Daisuke
authors: D
authors: Tsuji
authors: Ahmad
authors: A
authors: Azlina
authors: Mileva Ratko
authors: MR
authors: Karabasil
authors: Purevjav
authors: P
authors: Javkhlan
authors: Takahiro
authors: T
authors: Hasegawa
authors: Chenjuan
authors: C
authors: Yao
authors: Tetsuya
authors: T
authors: Akamatsu
authors: Kohji
authors: K
authors: Itoh
authors: Kazuo
authors: K
authors: Hosoi
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D002452:Cell Count; D049109:Cell Proliferation; D008026:Ligation; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012038:Regeneration; D018987:Salivary Ducts / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D012472:Salivation; D058985:Side-Population Cells / Q000166:cytology* / Q000378:metabolism; D013363:Submandibular Gland / Q000166:cytology / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins