title: Optimal culture conditions for constructing durable biografts for repairing the impaired heart--dynamic cell culture with pre-seeding.
abstract: BACKGROUND
  Tissue engineering with cell seeded biodegradable material has attracted attention as a novel means of treating the severely impaired heart. Here, we consider optimal preparation of a durable biograft using dynamic and static cultures.
  
  
  METHODS
  Vascular smooth muscle cells (VSMCs) derived from the rat aorta were seeded onto biodegradable material P (LA/CL) (poly-L-lactide-ε-caprolactone copolymer) and cultured as follows: a) Static culture (n = 11), b) dynamic culture (n = 12), c) 0 h pre-seeding (n = 12), d) 24 h pre-seeding (n = 5) and e) 1 week pre-seeding (n = 12). Dynamic culture: Cells were cultured in spinner flasks. Pre-seeding: Static cell seeding and culture before dynamic culture.
  
  
  RESULTS
  The conditions of the P (LA/CL) in the five groups were evaluated as cell proliferation and by histological studies.
  
  
  RESULTS
  VSMCs proliferated both in and on the biodegradable materials. The quality of the dynamic culture cell with pre-seeding increased. Although the duration of pre-seeding exerted no significantly different effects, cell attachment and proliferation were widely scattered in the 0 h pre-seeding group, whereas cells proliferating on the front of the scaffold obstructed proliferation inside the biodegradable material in the 1 week pre-seeding group .
  
  
  CONCLUSIONS
  Dynamic cell culture with 24 h pre-seeding is effective for constructing ideal biografts.
authors: Division of Cardiovascular Surgery, Department of Surgery, School of Medicine, Faculty of Medicine, Toho University, Tokyo, Japan.
authors: Hiroki
authors: H
authors: Yokomuro
authors: Noritsugu
authors: N
authors: Shiono
authors: Yoshinori
authors: Y
authors: Watanabe
authors: Katsunori
authors: K
authors: Yoshihara
authors: Nobuya
authors: N
authors: Koyama
authors: Mitsumasa
authors: M
authors: Okada
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D006348:Cardiac Surgical Procedures; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D006331:Heart Diseases / Q000473:pathology / Q000601:surgery*; D009038:Motion; D009131:Muscle, Smooth, Vascular / Q000502:physiology* / Q000637:transplantation; D032389:Myocytes, Smooth Muscle / Q000502:physiology* / Q000637:transplantation; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C062968:lactide-caprolactone copolymer