title: Electron microscopic imaging of integrin.
abstract: Rotary-shadowed samples often used for electron microscopy do not preserve native integrin conformations. Negatively stained integrins - or, more desirably, unstained integrins in a cryo-condition - are now being used with sophisticated imaging techniques. Additionally, a single-particle analysis (SPA) of integrins is advanced by the recent determination of several crystal structures of integrins. Nevertheless the conformational flexibility of integrins limits the ability of SPA to image physiologic conformations. To solve this problem, we apply electron tomography to purified integrin, thereby obtaining high-quality three-dimensional (3-D) images that fit well to the atomic structures. We have also taken typical SPA approaches to obtain a 3-D reconstruction of integrin, using conditions that favor the bent conformation.
authors: Research Center for Structural and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Osaka, Japan. ikenji@protein.osaka-u.ac.jp
authors: Kenji
authors: K
authors: Iwasaki
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D016023:Integrins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D016624:Negative Staining
chemical_list: D016023:Integrins