title: Suppression of bovine viral diarrhea virus replication by single and dual short hairpin RNA-mediated RNA interference.
abstract: Bovine viral diarrhea virus (BVDV) is one of the most important pathogens to the cattle industry, causing a significant economic loss throughout the world. Despite the wide use of various control measures for BVDV, the disease remains prevalent. In this study, we achieved an efficient inhibition of NADL strain replication by plasmid-mediated shRNA targeting conserved regions of the viral genome. To further enhance the inhibiting efficiency, a dual shRNA expression plasmid, which could simultaneously express two different shRNA, was established and showed stronger inhibitory effects on virus replication. Moreover, the antiviral activity induced by the dual shRNA expression system was also evident on other BVDV-1 subgenotypes (BVDV-1a, BVDV-1b and BVDV-1c). Therefore, the dual shRNA system provides a more powerful strategy for inhibiting BVDV replication in a cross-resistance manner.
authors: Institute of Biotechnology, College of Animal Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
authors: Wei
authors: W
authors: Ni
authors: Shengwei
authors: S
authors: Hu
authors: Jun
authors: J
authors: Qiao
authors: Yuan
authors: Y
authors: Yu
authors: Dawei
authors: D
authors: Wang
authors: Qi
authors: Q
authors: Tong
authors: Yong
authors: Y
authors: Zhang
authors: Chuangfu
authors: C
authors: Chen
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000188:drug therapy / Q000821:virology; D002417:Cattle; D002460:Cell Line; D017124:Conserved Sequence / Q000187:drug effects / Q000235:genetics; D030241:Diarrhea Virus 1, Bovine Viral / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D005838:Genotype; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D014779:Virus Replication / Q000187:drug effects* / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering