title: Thermophilic helicase-dependent DNA amplification using the IsoAmp™ SE experimental kit for rapid detection of Streptococcus equi subspecies equi in clinical samples.
abstract: A simple and portable assay for detection of Streptococcus equi subspecies equi has been developed based on amplification of S. equi-specific sequence using a thermophilic helicase-dependent reaction followed by visual detection of the amplicon in a disposable lateral flow cassette. An experimental kit (IsoAmp™ SE) was evaluated. Analytical sensitivity was 50 copies of S. equi genomic DNA per reaction. The IsoAmp SE assay had 100% specificity when applied to nasal swabs and washes. The assay was more sensitive than culture but less sensitive than nested polymerase chain reaction (PCR). The test requires neither expensive equipment nor extensive training of personnel, provides a practical alternative to culture or PCR assays for detection of S. equi in clinical samples, and expedites identification of atypical colonies of S. equi and Streptococcus zooepidemicus in the laboratory.
authors: 1Sergey Artiushin, Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA. scarti1@email.uky.edu
authors: Sergey
authors: S
authors: Artiushin
authors: Yanhong
authors: Y
authors: Tong
authors: John
authors: J
authors: Timoney
authors: Bertrand
authors: B
authors: Lemieux
authors: Anne
authors: A
authors: Schlegel
authors: Huimin
authors: H
authors: Kong
mesh_terms: D000818:Animals; D004265:DNA Helicases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000175:diagnosis / Q000382:microbiology; D006736:Horses; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D018502:Streptococcus equi / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004265:DNA Helicases