title: Anti-inflammatory cembranoids from the Formosan soft coral Sinularia discrepans.
abstract: A new cembranoid, discrepanolide A (1), along with four known cembranoids 2-5 were isolated from the Formosan soft coral Sinularia discrepans. The structures of these compounds were determined by analysis of spectroscopic data and by comparison of NMR data with those of known compounds. None of these compounds were found to be cytotoxic towards a limited panel of cancer cell lines. Compounds 3-5 were found to display significant in vitro anti-inflammatory activity in LPS-stimulated RAW264.7 macrophage cells by inhibiting the expression of the iNOS protein. Compound 5 also significantly inhibited the accumulation of pro-inflammatory COX-2 protein.
authors: Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
authors: Yi
authors: Y
authors: Lu
authors: Huey-Jen
authors: HJ
authors: Su
authors: Yung-Husan
authors: YH
authors: Chen
authors: Zhi-Hong
authors: ZH
authors: Wen
authors: Jyh-Horng
authors: JH
authors: Sheu
authors: Jui-Hsin
authors: JH
authors: Su
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D003063:Cnidaria / Q000737:chemistry* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000633:toxicity; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008264:Macrophages / Q000201:enzymology / Q000378:metabolism; D015394:Molecular Structure; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004224:Diterpenes; D013778:Tetrazolium Salts; D013844:Thiazoles; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C022616:thiazolyl blue