title: A new high-performance liquid chromatography-tandem mass spectrometry method based on dispersive solid phase extraction for the determination of the mycotoxin fusarin C in corn ears and processed corn samples.
abstract: Fusarin C is a mycotoxin that is produced by a variety of Fusarium species and is therefore a possible contaminant in food and feed. For this reason, a reliable high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of fusarin C in food and feed samples was developed based on dispersive solid phase extraction (DSPE). This method has a limit of detection (LOD) of 2 μg/kg, a limit of quantitation (LOQ) of 7 μg/kg, and a recovery rate of 80%. Fifty different corn samples were analyzed, and fusarin C was detected in 40 of them. The fusarin C level varied in kernels of corn ears from not detectable up to 83 mg/kg and in food samples from not detectable up to 28 μg/kg. The co-occurrence of further structural analogues of fusarin C was confirmed by high-performance liquid chromatography Fourier transformation mass spectrometry (HPLC-FTMS). In addition, the stability of fusarin C under storage conditions was evaluated.
authors: Institute of Food Chemistry, University of Münster, Münster, Germany.
authors: Karin
authors: K
authors: Kleigrewe
authors: Anna-Carina
authors: AC
authors: Söhnel
authors: Hans-Ulrich
authors: HU
authors: Humpf
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D011090:Polyenes / Q000032:analysis*; D012639:Seeds / Q000737:chemistry; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D009183:Mycotoxins; D011090:Polyenes; C041403:fusarin C