title: Diagnostic value of meat juice in early detection of Classical swine fever virus infection.
abstract: To evaluate the diagnostic potential of meat juice for early detection of Classical swine fever virus (CSFV), meat juice and serum samples from pigs experimentally infected with different strains of CSFV were compared for virus load. From all samples, viral RNA was extracted by automated procedure before real-time reverse transcription polymerase chain reaction analysis was performed. Viral RNA was detected in meat juice, but at a lower level than in corresponding serum. Sensitivity was calculated to 91% and specificity to 97%. Disagreements between meat juice and serum results were found when samples originated from pigs infected with low virulence CSFV strains and/or when samples were collected within the first days after infection. In conclusion, while not the first choice for sample material for CSFV diagnosis, meat juice may constitute a useful alternative for herd-based studies or when blood and/or target organ material is not available. Strain virulence and time points for sample collection after infection are factors of importance for diagnostic success.
authors: Technical University of Denmark, National Veterinary Institute, Lindholm, DK-4771 Kalvehave, Denmark. loloh@vet.dtu.dk
authors: Louise
authors: L
authors: Lohse
authors: Ase
authors: A
authors: Uttenthal
authors: Thomas Bruun
authors: TB
authors: Rasmussen
authors: Jens
authors: J
authors: Nielsen
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000175:diagnosis*; D006692:Classical Swine Fever Virus / Q000302:isolation & purification* / Q000472:pathogenicity; D008460:Meat / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013552:Swine; D014774:Virulence
chemical_list: D012367:RNA, Viral