title: Electroosmotic flow in microchannels with nanostructures.
abstract: Here we report that nanopillar array structures have an intrinsic ability to suppress electroosmotic flow (EOF). Currently using glass chips for electrophoresis requires laborious surface coating to control EOF, which works as a counterflow to the electrophoresis mobility of negatively charged samples such as DNA and sodium dodecyl sulfate (SDS) denatured proteins. Due to the intrinsic ability of the nanopillar array to suppress the EOF, we carried out electrophoresis of SDS-protein complexes in nanopillar chips without adding any reagent to suppress protein adsorption and the EOF. We also show that the EOF profile inside a nanopillar region was deformed to an inverse parabolic flow. We used a combination of EOF measurements and fluorescence observations to compare EOF in microchannel, nanochannel, and nanopillar array chips. Our results of EOF measurements in micro- and nanochannel chips were in complete agreement with the conventional equation of the EOF mobility (μ(EOF-channel) = αC(i)(-0.5), where C(i) is the bulk concentration of the i-ions and α differs in micro- and nanochannels), whereas EOF in the nanopillar chips did not follow this equation. Therefore we developed a new modified form of the conventional EOF equation, μ(EOF-nanopillar) ≈ β[C(i) - (C(i)(2)/N(i))], where N(i) is the number of sites available to i-ions and β differs for each nanopillar chip because of different spacings or patterns, etc. The modified equation of the EOF mobility that we proposed here was in good agreement with our experimental results. In this equation, we showed that the charge density of the nanopillar region, that is, the total number of nanopillars inside the microchannel, affected the suppression of EOF, and the arrangement of nanopillars into a tilted or square array had no effect on it.
authors: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. yasui.takao@e.mbox.nagoya-u.ac.jp
authors: Takao
authors: T
authors: Yasui
authors: Noritada
authors: N
authors: Kaji
authors: Mohamad Reza
authors: MR
authors: Mohamadi
authors: Yukihiro
authors: Y
authors: Okamoto
authors: Manabu
authors: M
authors: Tokeshi
authors: Yasuhiro
authors: Y
authors: Horiike
authors: Yoshinobu
authors: Y
authors: Baba
mesh_terms: D053841:Electroosmosis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D055616:Microtechnology / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011506:Proteins