title: [Effects of yin qiaojiedu soft capsule on influenza virus load and M1 expression in mice].
abstract: This study is to investigate the treatment of YinQiaojiedu soft capsule for influenza virus A/PR8/34 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, and the lung index and death rate were observed. Real time RT-PCR and Western blotting technique were used to detect the virus load and the relative expression of M1 protein in lungs of mice on the 1st, 3rd, 5th and 7th day after infection. The results showed that YinQiaojiedu soft capsule in 1 g x kg(-1) and 0.5 g x kg(-1) dose groups can decrease the lung index significantly on the 3rd, 5th and 7th day after being infected (P < 0.05, P < 0.01), and the number of death in the two groups of animals decreased significantly. YinQiaojiedu soft capsule in 1 g x kg(-1) dose group can decreased virus load at each time point, and lower it in 0.5 g x kg(-1) dose group at the 3rd, 5th and 7th day (P < 0.05, P < 0.01). YinQiaojiedu soft capsule can decrease the relative expression of M1 protein in lungs of mice, 1 g x kg(-1) and 0.5 g x kg(-1) dose groups are significantly lower in expression of M1 protein compared with model group at the 3rd and 7th day (P < 0.05, P < 0.01). It can be concluded that YinQiaojiedu soft capsule exerts antiviral effects against influenza virus by downregulating expression of virus load and M1 protein.
authors: Capital Medical University, Beijing 100069, China.
authors: Ying
authors: Y
authors: Liu
authors: Yu-Jing
authors: YJ
authors: Shi
authors: Han
authors: H
authors: Shi
authors: Ju-Ying
authors: JY
authors: Zhong
authors: Fang-Zhou
authors: FZ
authors: Liu
authors: Ying-Jie
authors: YJ
authors: Gao
authors: Ya-Hong
authors: YH
authors: Jin
authors: Shan-Shan
authors: SS
authors: Guo
authors: Xiao-Lan
authors: XL
authors: Cui
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002214:Capsules; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D053118:Influenza A Virus, H1N1 Subtype; D008168:Lung / Q000378:metabolism / Q000821:virology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009976:Orthomyxoviridae Infections / Q000188:drug therapy* / Q000378:metabolism / Q000821:virology; D011014:Pneumonia / Q000378:metabolism* / Q000821:virology; D019562:Viral Load / Q000187:drug effects*; D014763:Viral Matrix Proteins / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D002214:Capsules; D004365:Drugs, Chinese Herbal; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins