title: A PVP-extract fungal protein of Omphalia lapideacens and its antitumor             activity on human gastric tumors and normal cells.
abstract: Omphalia lapidescens is an important medicinal fungus as well as traditional             Chinese medicine used for disease treatment. It is mainly used as a vermifuge             for anthelmintic therapy, but it has not been hitherto reported to possess antitumor             activity. In this study, a purified bioactive protein in O. lapidescens (pPeOp)             was obtained using polyvinylpyrrolidone (PVP) followed by gel filtration chromatography.             To evaluate the in vitro antitumor activity of pPeOp in human gastric tumor cells             (MC-4 and SGC-7901) and normal cells (MC-1), MTT assay and FCM assay were used             and the morphological changes, cell viability, cell death rate and cell apoptosis             rate of MC-4, SGC-7901 and MC-1 cells were estimated. The results showed that             pPeOp could significantly reduce the cell viability of MC-4 and SGC-7901 cells             in a concentration-dependent manner, with IC50 values of 236.05 and 156.28 µg/ml,             respectively. The morphological observation also indicated a similar result. In             FCM assays, a significant increase of cell death rate and cell apoptosis rate             of the tumor cells were observed, indicating probable necrosis-inducing effects             and/or apoptosis-inducing effects of pPeOp. Importantly, there was no significant             effect of pPeOp on MC-1 cells in each assay, showing that pPeOp has no adverse             effects on the normal cells. In conclusion, pPeOp is a newly discovered bioactive             protein in O. lapidescens and this is the first report on antitumor activity of             such a fungal protein. This may provide a meaningful basis for developing a new             protein drug for treatment against cancer, especially gastric cancer.
authors: Zhejiang Chinese Medical University, Hangzhou, PR China.
authors: Yi-Tao
authors: YT
authors: Chen
authors: Qun-Ying
authors: QY
authors: Lu
authors: Mei-Ai
authors: MA
authors: Lin
authors: Dong-Qing
authors: DQ
authors: Cheng
authors: Zhi-Shan
authors: ZS
authors: Ding
authors: Le-Tian
authors: LT
authors: Shan
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005434:Flow Cytometry; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D008516:Medicine, Chinese Traditional; D011205:Povidone / Q000737:chemistry*; D013274:Stomach Neoplasms; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins; D011205:Povidone