title: New luminescent bioprobes Eu(lll)-phloroglucinol derivatives and their spectrofluorimetric, electrochemical interactions with nucleotides and DNA.
abstract: Two new ligands derived from phloroglucinol 2-{[(4-methoxy benzoyl)oxy]} methyl benzoic acid[L1] and 2-{[(4-methyl benzoyl)oxy] methyl} benzoic acid[L2] were synthesized. The solid complex Eu(III)-L2 has been synthesised and characterized by elemental analysis, UV and IR spectra. The reaction of Eu(III) with the two synthesized ligands has been investigated in I = 0.1 mol dm(-3) p-toluene sulfonate by cyclic voltammetry and square wave voltammetry. The reaction of Eu (III)-L1 and Eu (III)-L2 binary complexes with nucleotide 5'-AMP, 5'-ADP, 5'-ATP, 5'- GMP, 5'-IMP, and 5'-CMP has been investigated using UV, fluorescence and electrochemical methods. The experimental conditions were selected such that self-association of the nucleotides and their complexes was negligibly small, that is, the monomeric complexes were studied. The interaction of the Eu(III)-L1 or L2 solid complexes with calf-thymus DNA has been investigated by fluorescence and electrochemical methods including cyclic voltammetery(CV), differential pulse polarography (DPP) and square wave voltammetry (SWV) on a glassy carbon electrode. The fluorescence intensity of Eu(III)-L2 complex was enhanced with the addition of DNA. Under optimal conditions in phosphate buffer pH 7.0 at 25 °C the linear range is 3-20 μM for calf thymus DNA (CT-DNA) and the corresponding determination limit is 1.8 μM.
authors: Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt. azab2@yahoo.com
authors: Hassan Ahmed
authors: HA
authors: Azab
authors: Zeinab M
authors: ZM
authors: Anwar
authors: Enas T
authors: ET
authors: Abdel-Salam
authors: Mahmoud
authors: M
authors: el-Sayed-Sebak
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004563:Electrochemistry; D005063:Europium / Q000737:chemistry*; D049408:Luminescent Agents / Q000737:chemistry / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism*; D010696:Phloroglucinol / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D049408:Luminescent Agents; D009711:Nucleotides; D009942:Organometallic Compounds; D005063:Europium; D004247:DNA; D010696:Phloroglucinol