title: Habits with killer instincts: in vivo analysis on the severity of oral mucosal alterations using autofluorescence spectroscopy.
abstract: Oral habits like chewing and smoking are main causes of oral cancer, which has a higher mortality rate than many other cancer forms. Currently, the long term survival rate of oral cancer is less than 50%, as a majority of cases are detected very late. The clinician's main challenge is to differentiate among a multitude of red, white, or ulcerated lesions. Hence, new noninvasive, reliable, and fast techniques for the discrimination of oral cavity disorders are to be developed. This study includes autofluorescence spectroscopic screening of normal volunteers with and without lifestyle oral habits and patients with oral submucous fibrosis (OSF). The spectra from different sites of habitués, non-habitués, and OSF patients were analyzed using the intensity ratio, redox ratio, and linear discriminant analysis (LDA). The spectral disparities among these groups are well demonstrated in the emission regions of collagen and Flavin adenine dinucleotide. We observed that LDA gives better efficiency of classification than the intensity ratio technique. Even the differentiation of habitués and non-habitués could be well established with LDA. The study concludes that the clinical application of autofluorescence spectroscopy along with LDA, yields spontaneous screening among individuals, facilitating better patient management for clinicians and better quality of life for patients.
authors: Sree Chitra Tirunal Institute for Medical Sciences & Technology, Biophotonics and Molecular Imaging Lab, Biomedical Technology Wing, Poojappura, Thiruvananthapuram - 695 012, Kerala, India.
authors: S
authors: S
authors: Nazeer Shaiju
authors: Saraswathy
authors: S
authors: Ariya
authors: Rajasekharan
authors: R
authors: Asish
authors: Padippurakkakath
authors: P
authors: Salim Haris
authors: Balan
authors: B
authors: Anita
authors: Gupta
authors: G
authors: Arun Kumar
authors: Ramapurath S
authors: RS
authors: Jayasree
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D003094:Collagen / Q000737:chemistry; D016002:Discriminant Analysis; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry; D006184:Habits; D006801:Humans; D008297:Male; D008875:Middle Aged; D009059:Mouth Diseases / Q000473:pathology*; D009061:Mouth Mucosa / Q000737:chemistry / Q000473:pathology*; D009914:Oral Submucous Fibrosis / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D012907:Smoking / Q000378:metabolism / Q000473:pathology; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005182:Flavin-Adenine Dinucleotide; D003094:Collagen