title: Exploring the strength, mode, dynamics, and kinetics of binding interaction of a cationic biological photosensitizer with DNA: implication on dissociation of the drug-DNA complex via detergent sequestration.
abstract: The present study aims at exploring a detailed characterization of the binding interaction of a promising cancer cell photosensitizer, harmane (HM), with DNA extracted from herring sperm. The polarity-sensitive prototropic transformation of HM, a naturally occurring, fluorescent, drug-binding alkaloid, β-carboline, is remarkably modified upon interaction with DNA and is manifested through significant modulations on the absorption and emission profiles of HM. From the series of studies undertaken in the present program, for example, absorption; steady-state emission; the effect of chaotrope (urea); iodide ion-induced steady-state fluorescence quenching; circular dichroism (CD); and helix melting from absorption spectroscopy; the mode of binding of HM into the DNA helix has been substantiated to be principally intercalative. Concomitantly, a discernible dependence of the photophysics of the DNA-bound drug on the medium ionic strength indicates that electrostatic attraction should not be ignored in the interaction. Efforts have also been delivered to delineate the dynamical aspects of the interaction, such as modulation in time-resolved fluorescence decay and rotational relaxation dynamics of the drug within the DNA environment. In view of the prospective biological applications of HM, the issue of facile dissociation of intercalated HM from the DNA helix also comprises a crucial prerequisite for the functioning as an effective therapeutic agent. In this context, our results imply that the concept of detergent-sequestered dissociation of the drug from the drug-DNA complex can be a prospective strategy through an appropriate choice of the detergent molecule. The utility of the present work resides in exploring the potential applicability of the fluorescence property of HM for studying its interactions with a relevant biological target, for example, DNA. In addition, the methods and techniques used in the present work can also be exploited to study the interaction of HM with other biological, biomimicking assemblies and drug delivery vehicles, and so forth.
authors: Department of Chemistry, University of Calcutta, 92 A. P. C. Road, Calcutta-700009, India.
authors: Bijan Kumar
authors: BK
authors: Paul
authors: Nikhil
authors: N
authors: Guchhait
mesh_terms: D002243:Carbolines / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D003902:Detergents / Q000737:chemistry*; D005454:Fluorescence Polarization; D006247:Harmine / Q000031:analogs & derivatives / Q000737:chemistry; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D009994:Osmolar Concentration; D017319:Photosensitizing Agents / Q000737:chemistry*; D055672:Static Electricity
chemical_list: D002243:Carbolines; D002412:Cations; D003902:Detergents; D017319:Photosensitizing Agents; D006247:Harmine; C005010:harman; D004247:DNA