title: Nanoparticle microinjection and Raman spectroscopy as tools for nanotoxicology studies.
abstract: Microinjection techniques and Raman spectroscopy have been combined to provide a new methodology to investigate the cytotoxic effects due to the interaction of nanomaterials with cells. In the present work, this novel technique has been used to investigate the effects of Ag and Fe(3)O(4) nanoparticles on Hela cells. The nanoparticles are microinjected inside the cells and these latter ones are probed by means of Raman spectroscopy after a short incubation time, in order to highlight the first and impulsive mechanisms developed by the cells to counteract the presence of the nanoparticles. The results put in evidence a different behaviour of the cells treated with nanoparticles in comparison with the control cells; these differences are supposed to be generated by an emerging oxidative stress due to the nanoparticles. The achieved results demonstrate the suitability of the proposed method as a new tool for nanotoxicity studies.
authors: BioNEM Laboratory, Experimental and Clinical Medicine Department, University Magna Graecia of Catanzaro, 88100 Loc. Germaneto, Catanzaro, Italy.
authors: Patrizio
authors: P
authors: Candeloro
authors: Luca
authors: L
authors: Tirinato
authors: Natalia
authors: N
authors: Malara
authors: Annalisa
authors: A
authors: Fregola
authors: Eudald
authors: E
authors: Casals
authors: Victor
authors: V
authors: Puntes
authors: Gerardo
authors: G
authors: Perozziello
authors: Francesco
authors: F
authors: Gentile
authors: Maria Laura
authors: ML
authors: Coluccio
authors: Gobind
authors: G
authors: Das
authors: Carlo
authors: C
authors: Liberale
authors: Francesco
authors: F
authors: De Angelis
authors: Enzo
authors: E
authors: Di Fabrizio
mesh_terms: D002460:Cell Line; D005290:Ferric Compounds / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D008845:Microinjections / Q000379:methods; D049329:Nanostructures; D018384:Oxidative Stress; D012834:Silver / Q000633:toxicity*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014116:Toxicology / Q000379:methods
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D012834:Silver