title: Controlling cell adhesion via replication of laser micro/nano-textured surfaces on polymers.
abstract: The aim of this study is to investigate cell adhesion and viability on highly rough polymeric surfaces with gradient roughness ratios and wettabilities prepared by microreplication of laser micro/nano-textured Si surfaces. Negative replicas on polydimethylsiloxane as well as positive ones on a photocurable (organically modified ceramic) and a biodegradable (poly(lactide-co-glycolide)) polymer have been successfully reproduced. The final culture substrates comprised from forests of micron-sized conical spikes exhibiting a range of roughness ratios and wettabilities, was achieved by changing the laser fluence used to fabricate the original template surfaces. Cell culture experiments were performed with the fibroblast NIH/3T3 and PC12 neuronal cell lines in order to investigate how these surfaces are capable of modulating different types of cellular responses including, viability, adhesion and morphology. The results showed a preferential adhesion of both cell types on the microstructured surfaces compared to the unstructured ones. In particular, the fibroblast NIH/3T3 cells show optimal adhesion for small roughness ratios, independent of the surface wettability and polymer type, indicating a non-monotonic dependence of cell adhesion on surface energy. In contrast, the PC12 cells were observed to adhere well to the patterned surfaces independent of the roughness ratio and wettability. These experimental findings are correlated with micromechanical measurements performed on the unstructured and replicated surfaces and discussed on the basis of previous observations describing the relation of cell response to surface energy and rigidity.
authors: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology-Hellas (FORTH), 711 10, Heraklion, Crete, Greece.
authors: Niki
authors: N
authors: Koufaki
authors: Anthi
authors: A
authors: Ranella
authors: Katerina E
authors: KE
authors: Aifantis
authors: Marios
authors: M
authors: Barberoglou
authors: Stylianos
authors: S
authors: Psycharakis
authors: Costas
authors: C
authors: Fotakis
authors: Emmanuel
authors: E
authors: Stratakis
mesh_terms: D016475:3T3 Cells; D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D055119:Elastic Modulus; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D019344:Lactic Acid / Q000737:chemistry*; D007834:Lasers; D051379:Mice; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D016716:PC12 Cells; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D016596:Vinculin / Q000378:metabolism; D017155:Wettability
chemical_list: D000199:Actins; D004129:Dimethylpolysiloxanes; D016596:Vinculin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid