title: In vivo differentiation of adipose-derived stem cells in an injectable poloxamer-octapeptide hybrid hydrogel.
abstract: A hybrid hydrogel (PP) composed of Polomaxer-407 (PO) and octapeptide with amino acid sequence of KFEFKFEF (PE) was prepared to make a scaffold material incorporating PO's high and tunable mechanical strength and integrity with PE's superior bioactivity. Human adipose-derived mesenchymal stem cells (hASCs) were encapsulated into PE, PO and PP hydrogels respectively and injected subcutaneously at the dorsal neck area of nude mice. Adipose-like tissue regeneration was only observed in the mice injected with cell-encapsulated PP hydrogel. No adipose regeneration was found in the mice injected with PO or PE. Immunohistochemistry analysis with mouse anti-human nuclei monoclonal antibody demonstrated that the cells in the regenerated adipose-like tissue was originated from the injected hASCs. The growth of blood capillaries indicated that the regenerated adipose-like tissue was living tissue. In addition, human-originated cells were also found in nude mice skin. These cells were positive with mouse anti-human cells keratin antibody, suggesting that the injected hASCs migrated to the skin and differentiated into epithelial cells in vivo.
authors: Plastic Reconstructive Surgery & Medical Aesthetic Center, Nanjing University of TCM Affiliated Hospital, Nanjing, PR China.
authors: Jinlong
authors: J
authors: Huang
authors: Shukui
authors: S
authors: Wang
authors: Cui
authors: C
authors: Wei
authors: Yan
authors: Y
authors: Xu
authors: Ying
authors: Y
authors: Wang
authors: Jiyang
authors: J
authors: Jin
authors: Gaojun
authors: G
authors: Teng
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry / Q000494:pharmacology*; D007633:Keratins / Q000032:analysis; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D008819:Mice, Nude; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D020442:Poloxamer / Q000737:chemistry / Q000494:pharmacology*; D012038:Regeneration
chemical_list: D009842:Oligopeptides; D010455:Peptides; D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007633:Keratins