title: In vitro anti-platelet effects of simple plant-derived phenolic compounds are only found at high, non-physiological concentrations.
abstract: METHODS
  Bioactive polyphenols from fruits, vegetables, and beverages have anti-platelet effects and may thus affect the development of cardiovascular disease. We screened the effects of 26 low molecular weight phenolic compounds on two in vitro measures of human platelet function.
  
  
  RESULTS
  After platelets had been incubated with one of 26 low molecular weight phenolic compounds in vitro, collagen-induced human platelet aggregation and in vitro TRAP-induced P-selectin expression (as marker of platelet activation) were assessed. Incubation of platelet-rich plasma from healthy volunteers with 100 μmol/L hippuric acid, pyrogallol, catechol, or resorcinol significantly inhibited collagen-induced platelet aggregation (all p<0.05; n≥15). Incubation of whole blood with concentrations of 100 μmol/L salicylic acid, p-coumaric acid, caffeic acid, ferulic acid, 4-hydroxyphenylpropionyl glycine, 5-methoxysalicylic acid, and catechol significantly inhibited TRAP-induced surface P-selectin expression (all p<0.05; n=10). Incubation with lower concentrations of phenolics affected neither platelet aggregation nor activation.
  
  
  CONCLUSIONS
  As concentrations of 100 μmol/L are unlikely to be reached in the circulation, it is doubtful whether consumption of dietary phenolics in nutritionally attainable amounts plays a major role in inhibition of platelet activation and aggregation in humans.
authors: University of Aberdeen, Rowett Institute of Nutrition and Health, Aberdeen, UK.
authors: Luisa M
authors: LM
authors: Ostertag
authors: Niamh
authors: N
authors: O'Kennedy
authors: Graham W
authors: GW
authors: Horgan
authors: Paul A
authors: PA
authors: Kroon
authors: Garry G
authors: GG
authors: Duthie
authors: Baukje
authors: B
authors: de Roos
mesh_terms: D000328:Adult; D001565:Benzoates / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D001628:Beverages / Q000032:analysis; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002934:Cinnamates / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D005638:Fruit / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009994:Osmolar Concentration; D019007:P-Selectin / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000032:analysis / Q000494:pharmacology*; D059808:Polyphenols / Q000032:analysis / Q000494:pharmacology*; D014675:Vegetables / Q000737:chemistry; D055815:Young Adult
chemical_list: D001565:Benzoates; D002934:Cinnamates; D019007:P-Selectin; D010446:Peptide Fragments; D010975:Platelet Aggregation Inhibitors; D059808:Polyphenols; C546937:SELP protein, human; C086309:thrombin receptor peptide SFLLRNP