title: [Application of melanocytes and bone marrow mesenchymal stem cells in tissue engineered skin construction].
abstract: OBJECTIVE
  To explore the method of constructing tissue-engineered skin using melanocytes and bone marrow mesenchymal stem cells (BMSCs) in vivo.
  
  
  METHODS
  Melanocytes were isolated from human foreskin. BMSCs were isolated from human bone marrow. Both of them were co-cultured at a ratio of 1:10, and then were implanted into the collagen membrane to construct the tissue-engineered skin, which was applied for wound repair in nude mice. The effectiveness of wound repair and the distribution of melanocytes were evaluated by morphological observation, in vivo 4,6-diamidino-2-phenylindole, dihydrochloride (DAPI) fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.
  
  
  RESULTS
  The wounds were satisfactorily repaired among the nude mice. The melanocytes were distributed in the skin with normal structure, as confirmed by DAPI fluorescent staining tracing, HE staining, S-100 immunohistochemistry, and transmission electron microscopy.
  
  
  CONCLUSIONS
  Melanocytes and BMSCs, after proper in vitro culture at an appropriate ratio, can construct the tissue-engineered skin with I type collagen membrane.
authors: Department of Plastic Surgery, PUMC Hospital, Beijing, China.
authors: Ke-xin
authors: KX
authors: Song
authors: Qun
authors: Q
authors: Qiao
authors: Da-qing
authors: DQ
authors: Liu
authors: Xiao-jun
authors: XJ
authors: Wang
authors: Ru
authors: R
authors: Zhao
authors: Zhi-fei
authors: ZF
authors: Liu
authors: Xue-tao
authors: XT
authors: Pei
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D018920:Coculture Techniques; D024042:Collagen Type I; D006801:Humans; D008544:Melanocytes / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000293:injuries; D017768:Skin, Artificial; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I