title: Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.
abstract: Block copolymer-based vesicles have recently garnered a great deal of interest as nanoplatforms for drug delivery and molecular imaging applications due to their unique structural properties. These nanovesicles have been shown to direct their cargo to disease sites either through enhanced permeability and retention or even more efficiently via active targeting. Here, we show that the efficacy of nanovesicle targeting can be significantly improved when prepared from polymer-lipid blends compared with block copolymer alone. Polymer-lipid hybrid nanovesicles were produced from the aqueous coassembly of the diblock copolymer, poly(ethylene oxide)-block-polybutadiene (PEO-PBD), and the phospholipid, hydrogenated soy phosphatidylcholine (HSPC). The PEG-based vesicles, 117 nm in diameter, were functionalized with either folic acid or anti-HER2/neu affibodies as targeting ligands to confer specificity for cancer cells. Our results revealed that nanovesicles prepared from polymer-lipid blends led to significant improvement in cell binding compared to nanovesicles prepared from block copolymer alone in both in vitro cell studies and murine tumor models. Therefore, it is envisioned that nanovesicles composed of polymer-lipid blends may constitute a preferred embodiment for targeted drug delivery and molecular imaging applications.
authors: Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
authors: Zhiliang
authors: Z
authors: Cheng
authors: Drew R
authors: DR
authors: Elias
authors: Neha P
authors: NP
authors: Kamat
authors: Eric D
authors: ED
authors: Johnston
authors: Andrei
authors: A
authors: Poloukhtine
authors: Vladimir
authors: V
authors: Popik
authors: Daniel A
authors: DA
authors: Hammer
authors: Andrew
authors: A
authors: Tsourkas
mesh_terms: D000818:Animals; D002070:Butadienes / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D020724:Elastomers / Q000737:chemistry*; D005260:Female; D005492:Folic Acid / Q000737:chemistry; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D041681:NIH 3T3 Cells; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000188:drug therapy*; D010713:Phosphatidylcholines / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*
chemical_list: D002070:Butadienes; D020724:Elastomers; D005492:Folic Acid; D053769:Nanocapsules; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D018719:Erb-b2 Receptor Tyrosine Kinases; C028834:polybutadiene