title: Buffalo (Bubalus bubalis) epiphyseal proteins give protection from arsenic and fluoride-induced adverse changes in acetylcholinesterase activity in rats.
abstract: The objective of this study was to determine the effect of fluoride (F) and arsenic (As) on the activity of acetylcholinesterase (AChE), a critically important nervous system enzyme, and to test the protective role of buffalo epiphyseal (pineal) proteins (BEP) in rats. Arsenic (20 mg/kg BW, intraperitoneally) and F (150 ppm, perorally) were exposed, and BEP was administered intraperitoneally (100 μ g/kg BW) along with F and As to rats for 7 days. As and F exposure significantly (p < 0.05) increased their levels in plasma and decreased the activity of AChE in plasma, RBCs, heart, and brain of rats. Interestingly, As- and F-induced inhibition of AChE activities increased As and F levels in plasma, and organs were significantly (p < 0.05) counteracted by BEP administration. These findings indicate the protective role of buffalo (Bubalus bubalis) epiphyseal proteins on F- and As-induced adverse changes in AChE activity as a candidate biomarker for neurotoxicity in female rats.
authors: Neurophysiology Laboratory, Division of Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India. vijaykbharti@rediffmail.com
authors: Vijay K
authors: VK
authors: Bharti
authors: R S
authors: RS
authors: Srivastava
authors: Ajay K
authors: AK
authors: Anand
authors: K
authors: K
authors: Kusum
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood* / Q000378:metabolism; D000818:Animals; D001151:Arsenic / Q000493:pharmacokinetics / Q000633:toxicity*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002020:Buffaloes; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D005260:Female; D005459:Fluorides / Q000493:pharmacokinetics / Q000633:toxicity*; D006321:Heart / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D009479:Neuropeptides / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010870:Pineal Gland / Q000737:chemistry*; D051381:Rats
chemical_list: D009479:Neuropeptides; D018696:Neuroprotective Agents; D000110:Acetylcholinesterase; D001151:Arsenic; D005459:Fluorides