title: Immunomodulative efficacy of bone marrow-derived mesenchymal stem cells cultured in human platelet lysate.
abstract: Human mesenchymal stem cells (hMSCs) are considered to be a promising tool for novel cell-based therapies. Clinical applications in solid organ transplantation were hampered by the dependence on animal serum for hMSCs clinical scale expansion until substitution with human platelet lysate (HPL) became a promising alternative. Therefore we focused on a direct comparison of immunomodulatory properties of hMSCs cultured in HPL or fetal calf serum (FCS). Phenotypic characterization, detection of cytokine secretion and effects on alloantigen- and mitogen-induced lymphocyte proliferation as well as degranulation of cytomegalovirus-specific cytotoxic T cells were applied in potency assays. We demonstrated that HPL-cultured MSCs have comparable immunomodulatory capacities to their FCS-cultured counterparts. The observed immunomodulatory properties include a beneficial inhibitory effect on immune cell proliferation and an unaffected viral T cell immunity. Thus, culturing hMSCs in HPL generates an efficient and safe expansion combined with intriguing immunomodulatory properties making these cells an attractive cell therapeutic tool.
authors: Institute of Medical Immunology, Universitätsmedizin Charité, Campus Virchow Klinikum, Augustenburger Platz 1, 13353, Berlin, Germany.
authors: Antoinette
authors: A
authors: Flemming
authors: Katharina
authors: K
authors: Schallmoser
authors: Dirk
authors: D
authors: Strunk
authors: Meaghan
authors: M
authors: Stolk
authors: Hans-Dieter
authors: HD
authors: Volk
authors: Martina
authors: M
authors: Seifert
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001792:Blood Platelets / Q000378:metabolism*; D001853:Bone Marrow / Q000473:pathology; D002417:Cattle; D002457:Cell Extracts / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000378:metabolism; D003587:Cytomegalovirus / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D056747:Immunomodulation; D007519:Isoantigens / Q000276:immunology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D044967:Serum / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D000956:Antigens, Viral; D002457:Cell Extracts; D003470:Culture Media; D007519:Isoantigens