title: A self-immobilizing and fluorogenic unnatural amino acid that mimics phosphotyrosine.
abstract: Synthesis of the first self-immobilizing, fluorogenic unnatural amino acid that mimics phosphotyrosine (pTyr) is reported. By using solid-phase peptide synthesis, it was subsequently incorporated into peptide-based probes which found applications in bioimaging and fluorescence-activated cell sorting (FACS).
authors: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors: Jingyan
authors: J
authors: Ge
authors: Lin
authors: L
authors: Li
authors: Shao Q
authors: SQ
authors: Yao
mesh_terms: D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D019000:Phosphotyrosine / Q000737:chemistry*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D019000:Phosphotyrosine; D017027:Protein Tyrosine Phosphatases