title: Cytochrome c Trp65Ser substitution results in inhibition of acetic acid-induced programmed cell death in Saccharomyces cerevisiae.
abstract: To gain further insight into the role of cytochrome c (cyt c) in yeast programmed cell death induced by acetic acid (AA-PCD), comparison was made between wild type and two mutant cells, one lacking cyt c and the other (W65Scyc1) expressing a mutant iso-1-cyt c in a form unable to reduce cyt c oxidase, with respect to occurrence of AA-PCD, cyt c release, ROS production and caspase-like activity. We show that in W65Scyc1 cells: i. no release of mutant cyt c occurs with inhibition of W65Scyc1 cell AA-PCD shown to be independent on impairment of electron flow, ii. there is a decrease in ROS production and an increase in caspase-like activity. We conclude that cyt c release does not depend on cyt c function as an electron carrier and that when still associated to the mitochondrial membrane, cyt c in its reduced form has a role in AA-PCD, by regulating ROS production and caspase-like activity.
authors: CNR, Istituto di Biomembrane e Bioenergetica, I-70126 Bari, Italy. n.guaragnella@ibbe.cnr.it
authors: Nicoletta
authors: N
authors: Guaragnella
authors: Salvatore
authors: S
authors: Passarella
authors: Ersilia
authors: E
authors: Marra
authors: Sergio
authors: S
authors: Giannattasio
mesh_terms: D019342:Acetic Acid / Q000633:toxicity*; D019943:Amino Acid Substitution; D020169:Caspases / Q000378:metabolism; D016923:Cell Death; D045304:Cytochromes c / Q000235:genetics* / Q000378:metabolism; D020125:Mutation, Missense; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C014059:CYC1 protein, S cerevisiae; D017382:Reactive Oxygen Species; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D020169:Caspases; D019342:Acetic Acid