title: (-)-epigallocatechin-3-O-gallate augments pentobarbital-induced sleeping behaviors through Cl- channel activation.
abstract: This experiment investigated whether (-)-epigallocatechin-3-O-gallate (EGCG) (5-20 mg/kg, p.o.) has hypnotic effects and/or enhances pentobarbital-induced sleeping behaviors and whether these effects are mediated by γ-aminobutyric acid (GABA) receptors. EGCG prolonged sleeping time induced by pentobarbital (42 mg/kg, i.p.) and reduced sleeping latency induced by pentobarbital similarly to muscimol (0.2 mg/kg, i.p.), a GABA(A) receptor agonist in mice. EGCG also increased sleeping rate and sleeping time when co-administered with pentobarbital (28 mg/kg, i.p.) at a subhypnotic dosage. In addition, EGCG and pentobarbital increased chloride (Cl(-)) influx in primary cultured cerebellar cells. EGCG and pentobarbital decreased GABA(A) receptors α-subunit expression and had no effect on the expression of β- and γ-subunits and of glutamic acid decarboxylase in the hippocampus of rats. In conclusion, the EGCG enhancement of Cl(-) influx may play an important role in pentobarbital-induced sleeping behaviors.
authors: Medical Research Center (MRCT), College of Pharmacy, Chungbuk National University, Cheongju, Korea.
authors: Kwang-Soon
authors: KS
authors: Park
authors: Jin-Yi
authors: JY
authors: Han
authors: Dong-Cheul
authors: DC
authors: Moon
authors: Jin Tae
authors: JT
authors: Hong
authors: Ki-Wan
authors: KW
authors: Oh
mesh_terms: D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009118:Muscimol / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018079:Receptors, GABA / Q000378:metabolism; D012890:Sleep / Q000187:drug effects*
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D018696:Neuroprotective Agents; D018079:Receptors, GABA; D009118:Muscimol; D002392:Catechin; C045651:epigallocatechin gallate; D005968:Glutamate Decarboxylase; D010424:Pentobarbital