title: Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae.
abstract: A case report of ventriculoperitoneal shunt infection caused by Candida lusitaniae in a 6-year-old patient with cerebral astrocytoma and obstructive hydrocephalus is presented briefly with emphasis on the course of antifungal treatment. Seven isolates recovered subsequently from the cerebrospinal fluid were studied retrospectively. To confirm identity, isolates were typed using pulsed-field gel electrophoresis and melting curve of random amplified polymorphic DNA (McRAPD). Further, the ability to form biofilm and its susceptibility to systemic antifungals were evaluated. Using McRAPD, identity of C. lusitaniae isolates showing slight microevolutionary changes in karyotypes was undoubtedly confirmed; successful application of numerical interpretation of McRAPD for typing is demonstrated here for the first time. The strain was also recognized as a strong biofilm producer. Moreover, minimum biofilm inhibitory concentrations were very high, in contrast to low antifungal minimum inhibitory concentrations of isolates. It can be concluded that McRAPD seems to be a simple and reliable method not only for identification but also for typing of yeasts. A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely caused treatment failure.
authors: Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hnevotinska 3, 77515, Olomouc, Czech Republic. petr_hamal@yahoo.com
authors: Petr
authors: P
authors: Hamal
authors: Juraj
authors: J
authors: Hanzen
authors: Frantisek
authors: F
authors: Horn
authors: Jitka
authors: J
authors: Trtkova
authors: Lenka
authors: L
authors: Ruskova
authors: Renata
authors: R
authors: Vecerova
authors: Filip
authors: F
authors: Ruzicka
authors: Anna
authors: A
authors: Vollekova
authors: Vladislav
authors: V
authors: Raclavsky
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D001254:Astrocytoma / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D002175:Candida / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D002648:Child; D003428:Cross Infection / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D006849:Hydrocephalus / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D008297:Male; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques; D009691:Nucleic Acid Denaturation; D019105:Random Amplified Polymorphic DNA Technique; D017211:Treatment Failure; D017287:Ventriculoperitoneal Shunt / Q000009:adverse effects
chemical_list: D000935:Antifungal Agents