title: Mathematical evaluation of the amino acid and polyphenol content and antioxidant activities of fruits from different apricot cultivars.
abstract: Functional foods are of interest because of their significant effects on human health, which can be connected with the presence of some biologically important compounds. In this study, we carried out complex analysis of 239 apricot cultivars (Prunus armeniaca L.) cultivated in Lednice (climatic area T4), South Moravia, Czech Republic. Almost all previously published studies have focused only on analysis of certain parameters. However, we focused on detection both primary and secondary metabolites in a selection of apricot cultivars with respect to their biological activity. The contents of thirteen biogenic alpha-L-amino acids (arginine, asparagine, isoleucine, lysine, serine, threonine, valine, leucine, phenylalanine, tryptophan, tyrosine, proline and alanine) were determined using ion exchange chromatography with UV-Vis spectrometry detection. Profile of polyphenols, measured as content of ten polyphenols with significant antioxidant properties (gallic acid, procatechinic acid, p-aminobenzoic acid, chlorogenic acid, caffeic acid, vanillin, p-coumaric acid, rutin, ferrulic acid and quercetrin), was determined by high performance liquid chromatography with spectrometric/electrochemical detection. Moreover, content of total phenolics was determined spectrophotometrically using the Folin-Ciocalteu method. Antioxidant activity was determined using five independent spectrophotometric methods: DPPH assay, DMPD method, ABTS method, FRAP and Free Radicals methods. Considering the complexity of the obtained data, they were processed and correlated using bioinformatics techniques (cluster analysis, principal component analysis). The studied apricot cultivars were clustered according to their common biochemical properties, which has not been done before. The observed similarities and differences were discussed.
authors: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.
authors: Jiri
authors: J
authors: Sochor
authors: Helena
authors: H
authors: Skutkova
authors: Petr
authors: P
authors: Babula
authors: Ondrej
authors: O
authors: Zitka
authors: Natalia
authors: N
authors: Cernei
authors: Otakar
authors: O
authors: Rop
authors: Boris
authors: B
authors: Krska
authors: Vojtech
authors: V
authors: Adam
authors: Ivo
authors: I
authors: Provazník
authors: Rene
authors: R
authors: Kizek
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D016000:Cluster Analysis; D019295:Computational Biology; D005609:Free Radicals / Q000737:chemistry; D005638:Fruit / Q000737:chemistry*; D005788:Gene Pool; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D025341:Principal Component Analysis; D027861:Prunus / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl