title: MicroRNA profiling in subventricular zone after stroke: MiR-124a regulates proliferation of neural progenitor cells through Notch signaling pathway.
abstract: BACKGROUND
  The Notch signaling pathway regulates adult neurogenesis under physiological and pathophysiological conditions. MicroRNAs are small non-coding RNA molecules that regulate gene expression. The present study investigated the effect of miR-124a on the Notch signaling pathway in stroke-induced neurogenesis.
  
  
  RESULTS
  We found that adult rats subjected to focal cerebral ischemia exhibited substantial reduction of miR-124a expression, a neuron specific miRNA, in the neural progenitor cells of the subventricular zone (SVZ) of the lateral ventricle, which was inversely associated with activation of Notch signals. In vitro, transfection of neural progenitor cells harvested from the SVZ of adult rat with miR-124a repressed Jagged-1 (JAG1), a ligand of Notch, in a luciferase construct containing the JAG1 target site. Introduction of miR-124a in neural progenitor cells significantly reduced JAG1 transcript and protein levels, leading to inactivation of Notch signals. Transfection of neural progenitor cells with miR-124a significantly reduced progenitor cell proliferation and promoted neuronal differentiation measured by an increase in the number of Doublecortin positive cells, a marker of neuroblasts. Furthermore, introduction of miR-124a significantly increased p27Kip1 mRNA and protein levels, a downstream target gene of the Notch signaling pathway.
  
  
  CONCLUSIONS
  Collectively, our study demonstrated that in vivo, stroke alters miRNA expression in SVZ neural progenitor cells and that in vitro, miR-124a mediates stroke-induced neurogenesis by targeting the JAG-Notch signaling pathway.
authors: Department of Neurology, Henry Ford Hospital, Detroit, Michigan, United States of America.
authors: Xian Shuang
authors: XS
authors: Liu
authors: Michael
authors: M
authors: Chopp
authors: Rui Lan
authors: RL
authors: Zhang
authors: Tang
authors: T
authors: Tao
authors: Xin Li
authors: XL
authors: Wang
authors: Haifa
authors: H
authors: Kassis
authors: Ann
authors: A
authors: Hozeska-Solgot
authors: Li
authors: L
authors: Zhang
authors: Charles
authors: C
authors: Chen
authors: Zheng Gang
authors: ZG
authors: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002552:Cerebral Ventricles / Q000378:metabolism* / Q000473:pathology; D000089662:Doublecortin Protein; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020244:Infarction, Middle Cerebral Artery; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D058953:Neural Stem Cells / Q000378:metabolism* / Q000473:pathology; D055495:Neurogenesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D051880:Receptors, Notch / Q000378:metabolism*; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics; D020521:Stroke / Q000235:genetics* / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; C000714848:Dcx protein, rat; D000089662:Doublecortin Protein; C548144:MIRN124 microRNA, rat; D035683:MicroRNAs; D051880:Receptors, Notch