title: Experimental arthritis triggers periodontal disease in mice: involvement of TNF-α and the oral Microbiota.
abstract: Rheumatoid arthritis (RA) and periodontal disease (PD) are prevalent chronic inflammatory disorders that affect bone structures. Individuals with RA are more likely to experience PD, but how disease in joints could induce PD remains unknown. This study aimed to experimentally mimic clinical parameters of RA-induced PD and to provide mechanistic findings to explain this association. Chronic Ag-induced arthritis (AIA) was triggered by injection of methylated BSA in the knee joint of immunized mice. Anti-TNF-α was used to assess the role of this cytokine. Intra-articular challenge induced infiltration of cells, synovial hyperplasia, bone resorption, proteoglycan loss, and increased expression of cytokines exclusively in challenged joints. Simultaneously, AIA resulted in severe alveolar bone loss, migration of osteoclasts, and release of proinflammatory cytokines in maxillae. Anti-TNF-α therapy prevented the development of both AIA and PD. AIA did not modify bacterial counts in the oral cavity. PD, but not AIA, induced by injection of Ag in immunized mice was decreased by local treatment with antiseptic, which decreased the oral microbiota. AIA was associated with an increase in serum C-reactive protein levels and the expression of the transcription factors RORγ and Foxp3 in cervical lymph nodes. There were higher titers of anti-collagen I IgG, and splenocytes were more responsive to collagen I in AIA mice. In conclusion, AIA-induced PD was dependent on TNF-α and the oral microbiota. Moreover, PD was associated with changes in expression of lymphocyte transcription factors, presence of anti-collagen Abs, and increased reactivity to autoantigens.
authors: Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
authors: Celso Martins
authors: CM
authors: Queiroz-Junior
authors: Mila Fernandes Moreira
authors: MF
authors: Madeira
authors: Fernanda Matos
authors: FM
authors: Coelho
authors: Vivian Vasconcelos
authors: VV
authors: Costa
authors: Rafaela Leal Costa
authors: RL
authors: Bessoni
authors: Larissa Fonseca da Cunha
authors: LF
authors: Sousa
authors: Gustavo Pompermaier
authors: GP
authors: Garlet
authors: Danielle da Glória de
authors: Dda G
authors: Souza
authors: Mauro Martins
authors: MM
authors: Teixeira
authors: Tarcília Aparecida da
authors: TA
authors: Silva
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009055:Mouth / Q000382:microbiology*; D010510:Periodontal Diseases / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha