title: Antibacterial surfaces based on polymer brushes: investigation on the influence of brush properties on antimicrobial peptide immobilization and antimicrobial activity.
abstract: Primary amine containing copolymer, poly(N,N-dimethylacrylamide-co-N-(3-aminopropyl)methacrylamide hydrochloride) (poly(DMA-co-APMA)), brushes were synthesized on Ti surface by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous conditions. A series of poly(DMA-co-APMA) copolymer brushes on titanium (Ti) surface with different molecular weights, thicknesses, compositions, and graft densities were synthesized by changing the SI-ATRP reaction conditions. Cysteine-functionalized cationic antimicrobial peptide Tet213 (KRWWKWWRRC) was conjugated to the copolymers brushes using a maleimide-thiol addition reaction after initial modification of the grafted chains using 3-maleimidopropionic acid N-hydroxysuccinimide ester. The modified surfaces were characterized by X-ray photoelectron spectroscopy (XPS), water contact angle measurements, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, atomic force microscopy (AFM), and ellipsometry analysis. The conjugation of the Tet213 onto brushes strongly depended on graft density of the brushes at different copolymer brush compositions. The peptide density (peptides/nm(2)) on the surface varied with the initial composition of the copolymer brushes. Higher graft density of the brushes generated high peptide density (pepetide/nm(2)) and lower number of peptides/polymer chain and vice versa. The peptide density and graft density of the chains on surface greatly influenced the antimicrobial activity of peptide grafted polymer brushes against Pseudomonas aeruginosa.
authors: Centre for Blood Research and Department of Pathology and Laboratory Medicine, 2350 Health Sciences Mall, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.
authors: Guangzheng
authors: G
authors: Gao
authors: Kai
authors: K
authors: Yu
authors: Jason
authors: J
authors: Kindrachuk
authors: Donald E
authors: DE
authors: Brooks
authors: Robert E W
authors: RE
authors: Hancock
authors: Jayachandran N
authors: JN
authors: Kizhakkedathu
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D055767:Immobilized Proteins / Q000138:chemical synthesis* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011108:Polymers / Q000737:chemistry; D019736:Prostheses and Implants / Q000382:microbiology; D011552:Pseudomonas Infections / Q000382:microbiology / Q000517:prevention & control*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D017550:Spectroscopy, Fourier Transform Infrared; D013329:Structure-Activity Relationship; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D020099:Coated Materials, Biocompatible; D055767:Immobilized Proteins; D011108:Polymers; D013388:Succinimides; C099046:N,N-dimethylacrylamide; C001426:N-hydroxysuccinimide