title: Effects of impressic acid from Acanthopanax koreanum on NF-κB and PPARγ activities.
abstract: Impressic acid, 3α,11α-dihydroxylup-20(29)-en-28-oic acid, is a lupane-type triterpenoid isolated from Acanthopanax koreanum, which has been used as a Korean folk medicine for rheumatism, hepatitis, diabetes, and inflammatory disorders. Recently, it was reported that impressic acid has inhibitory effects on the LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells and on the nuclear factor of activated T-cells transcription factor activity. Thus, to investigate whether impressic acid has effects on the inhibition of nuclear factor kappa B (NF-κB) and activation of peroxisome proliferator-activated receptor gamma (PPARγ), luciferase reporter assays were used. The effects on the expression of NF-κB and PPARγ target genes were also examined by reverse transcription-polymerase chain reaction. In this study, impressic acid was found to inhibit tumor necrosis factor (TNF)-α induced NF-κB activity and to up-regulate transcriptional activity of PPARγ.
authors: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors: Jeong Ah
authors: JA
authors: Kim
authors: Seo Young
authors: SY
authors: Yang
authors: Seok Bean
authors: SB
authors: Song
authors: Young Ho
authors: YH
authors: Kim
mesh_terms: D001854:Bone Marrow Cells / Q000378:metabolism; D002470:Cell Survival; D002839:Chromans / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D003713:Dendritic Cells / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D028001:Eleutherococcus; D056945:Hep G2 Cells; D006801:Humans; D007249:Inflammation / Q000188:drug therapy; D008070:Lipopolysaccharides / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves; D012313:RNA / Q000032:analysis; D012216:Rheumatic Diseases / Q000188:drug therapy; D045162:Thiazolidinediones / Q000494:pharmacology; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics; D015533:Transcriptional Activation; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000077288:Troglitazone; D053978:Pentacyclic Triterpenes
chemical_list: D002839:Chromans; D016207:Cytokines; D008070:Lipopolysaccharides; D008156:Luciferases; D016328:NF-kappa B; D047495:PPAR gamma; D012313:RNA; D045162:Thiazolidinediones; D014157:Transcription Factors; D014315:Triterpenes; D000077288:Troglitazone; C570594:3,11-dihydroxylup-20(29)-en-28-oic acid; C480546:lupane; D053978:Pentacyclic Triterpenes