title: Identification of tumoral glial precursor cells in neuroblastoma.
abstract: Neuroblastic tumors (NBT) are composed by neuroblasts and Schwannian-like stroma. The origin of these two cell subtypes remains unclear. In this study, we describe, a neuroblastic-like subpopulation in neuroblastoma (NB) coexpressing GD2 and S100A6, neuroblastic and glial lineage markers respectively. The GD2(+)/S100A6(+) neuroblastic subpopulation was found to be enriched in low risk NB, distributed around the perivascular niche. Some stromal bundles showed GD2(+)/S100A6 costaining. Metastatic bone marrow specimens also showed GD2(+)/S100A6(+) cells. During in vitro retinoic acid induced differentiation of NB cell lines, rare GD2(+)/S100A6 neuroblatic cells appeared. We conclude that GD2(+)/S100A6(+) neuroblasts may represent a tumoral glial precursor subpopulation in NBT.
authors: Developmental Tumor Biology Laboratory, Hospital Sant Joan de Déu, Fundació Sant Joan de Déu, Barcelona, Spain.
authors: Sandra
authors: S
authors: Acosta
authors: Gemma
authors: G
authors: Mayol
authors: Eva
authors: E
authors: Rodríguez
authors: Cinzia
authors: C
authors: Lavarino
authors: Katleen
authors: K
authors: de Preter
authors: Candy
authors: C
authors: Kumps
authors: Idoia
authors: I
authors: Garcia
authors: Carmen
authors: C
authors: de Torres
authors: Jaume
authors: J
authors: Mora
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D018797:Cell Cycle Proteins / Q000096:biosynthesis; D002454:Cell Differentiation; D019070:Cell Lineage; D005732:Gangliosides / Q000096:biosynthesis; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D058953:Neural Stem Cells / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D009457:Neuroglia / Q000378:metabolism; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000096:biosynthesis; D012583:Schwann Cells / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D018797:Cell Cycle Proteins; D005732:Gangliosides; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C055351:S100A6 protein, human; C019403:ganglioside, GD2