title: Caloramator boliviensis sp. nov., a thermophilic, ethanol-producing bacterium isolated from a hot spring.
abstract: A novel moderately thermophilic, anaerobic, ethanol-producing bacterial strain, 45B(T), was isolated from a mixed sediment water sample collected from a hot spring at Potosi, Bolivia. The cells were straight to slightly curved rods approximately 2.5 µm long and 0.5 µm wide. The strain was Gram-stain-variable, spore-forming and monotrichously flagellated. Growth of the strain was observed at 45-65 °C and pH 5.5-8.0, with optima of 60 °C and pH 6.5. The substrates utilized by strain 45B(T) were xylose, cellobiose, glucose, arabinose, sucrose, lactose, maltose, fructose, galactose, mannose, glycerol, xylan, carboxymethylcellulose and yeast extract. The main fermentation product from xylose and cellobiose was ethanol (0.70 and 0.45 g ethanol per gram of consumed sugar, respectively). Acetate, lactate, propionate, carbon dioxide and hydrogen were also produced in minor quantities. 1,3-Propanediol was produced when glycerol-containing medium was supplemented with yeast extract. The major cellular fatty acids were anteiso-C(15:0), C(16:0), iso-C(16:0), C(15:1), iso-C(14:0), C(13:0) and C(14:0). The polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminoglycolipid and 15 other unidentified lipids were predominant. The DNA G+C content of strain 45B(T) was 32.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain 45B(T) is located within the Gram-type positive Bacillus-Clostridium branch of the phylogenetic tree. On the basis of morphological and physiological properties and phylogenetic analysis, strain 45B(T) represents a novel species, for which the name Caloramator boliviensis sp. nov. is proposed; the type strain is 45B(T) (=DSM 22065(T)=CCUG 57396(T)).
authors: Instituto de Investigaciones Fármaco Bioquímicas, Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés, PO Box 3239, La Paz, Bolivia.|Department of Biotechnology, Lund University, PO Box 124, S-22100 Lund, Sweden.
authors: Carla
authors: C
authors: Crespo
authors: Department of Biotechnology, Lund University, PO Box 124, S-22100 Lund, Sweden.
authors: Tania
authors: T
authors: Pozzo
authors: Department of Biotechnology, Lund University, PO Box 124, S-22100 Lund, Sweden.
authors: Eva
authors: E
authors: Nordberg Karlsson
authors: Instituto de Investigaciones Fármaco Bioquímicas, Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés, PO Box 3239, La Paz, Bolivia.
authors: Maria Teresa
authors: MT
authors: Alvarez
authors: Department of Biotechnology, Lund University, PO Box 124, S-22100 Lund, Sweden.
authors: Bo
authors: B
authors: Mattiasson
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001838:Bolivia; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D006094:Gram-Positive Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008853:Microscopy; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D000431:Ethanol