title: Protein expression and purification of integrin I domains and IgSF ligands for crystallography.
abstract: Cell adhesion depends on combinational expression and interactions of a large number of adhesion molecules at cell-to-cell or cell-to-matrix contact sites. Integrins and their immunoglobulin superfamily (IgSF) ligands represent foremost classes of cell adhesion molecules in immune system. Structural study is critical for a better understanding of the interactions between integrins and their IgSF ligands. Here we describe protocols for protein expression of integrin αL I domain and its IgSF ligand ICAM-5 D1D2 fragment for crystallography.
authors: Department of Physiology, The University of Hong Kong, Hong Kong, SAR, China. hongmin@red.dfci.harvard.edu
authors: Hongmin
authors: H
authors: Zhang
authors: Jia-Huai
authors: JH
authors: Wang
mesh_terms: D000818:Animals; D001665:Binding Sites; D016466:CHO Cells; D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006224:Cricetinae; D003461:Crystallography; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008024:Ligands; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D008024:Ligands; D011994:Recombinant Proteins