title: Impaired peripheral Th1 CD4+ T cell response to Escherichia coli proteins in patients with Crohn's disease and ankylosing spondylitis.
abstract: BACKGROUND
  To clarify the impact of T cell responses towards enteric antigens for chronic intestinal inflammation, we determined T helper 1 reactivity towards conserved Escherichia coli proteins in patients with Crohn's disease (CD) and healthy individuals and patients with ankylosing spondylitis (AS), who also often show microscopic inflammatory lesions within the gut or even develop overt inflammatory bowel disease.
  
  
  METHODS
  We determined the frequency of IFNγ+CD40L+ cells/CD4+ T cells after stimulation of whole blood with pools of E. coli proteins.
  
  
  RESULTS
  The E. coli-specific Th1 response was significantly reduced in CD patients and to a lower extent also in AS patients.
  
  
  CONCLUSIONS
  E. coli is a target for polyclonal Th1 responses in healthy individuals. The impairment of these responses in CD and AS patients might be due to recruitment of enterobacteria-specific Th1 cells to the gut or might reflect inadequate priming of adaptive immune response.
authors: Klinik für Gastroenterologie, Infektiologie und Rheumatologie, Charité, Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, 12200, Berlin, Germany.
authors: Asgar
authors: A
authors: Ergin
authors: Uta
authors: U
authors: Syrbe
authors: Rebecca
authors: R
authors: Scheer
authors: Andreas
authors: A
authors: Thiel
authors: Thomas
authors: T
authors: Adam
authors: Konrad
authors: K
authors: Büssow
authors: Rainer
authors: R
authors: Duchmann
authors: Martin
authors: M
authors: Zeitz
authors: Joachim
authors: J
authors: Sieper
mesh_terms: D056704:Adaptive Immunity; D000293:Adolescent; D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D023201:CD40 Ligand / Q000378:metabolism; D002465:Cell Movement; D002648:Child; D002675:Child, Preschool; D003424:Crohn Disease / Q000276:immunology* / Q000503:physiopathology; D029968:Escherichia coli Proteins / Q000276:immunology*; D005260:Female; D006801:Humans; D007165:Immunosuppression Therapy; D007223:Infant; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D007422:Intestines / Q000473:pathology*; D008297:Male; D013167:Spondylitis, Ankylosing / Q000276:immunology* / Q000503:physiopathology; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D029968:Escherichia coli Proteins; D023201:CD40 Ligand; D007371:Interferon-gamma