title: Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice.
abstract: BACKGROUND
  Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral death in infants. Despite decades of research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches are therefore urgently needed to develop effective RSV vaccines.
  
  
  METHODS
  We developed viruslike particles (VLPs) consisting of an influenza virus matrix (M1) protein core and RSV-F or -G on the surface. We tested the immunogenicity and vaccine efficacy of these VLPs (RSV-F, RSV-G) in a mouse model.
  
  
  RESULTS
  Intramuscular vaccination with RSV-F or RSV-G VLPs elicited IgG2a dominant RSV-specific immunoglobulin G (IgG) antibody responses against RSV-A2 viruses in both serum and lung extract. Mice immunized with VLPs (RSV-F or RSV-G) showed higher viral neutralizing antibodies in vitro and significantly decreased lung virus loads in vivo after live RSV-A2 challenge. RSV-G VLPs showed better protective efficacy than RSV-F VLPs as determined by the levels of lung virus loads and morbidity postchallenge.
  
  
  CONCLUSIONS
  This study demonstrates that VLP vaccination provides effective protection against RSV infection. VLPs containing RSV-F and/or RSV-G are potential vaccine candidates against RSV.
authors: Zetra Biologicals LLC.
authors: Fu-Shi
authors: FS
authors: Quan
authors: Yonghwan
authors: Y
authors: Kim
authors: Sujin
authors: S
authors: Lee
authors: Hong
authors: H
authors: Yi
authors: Sang-Moo
authors: SM
authors: Kang
authors: Jadranka
authors: J
authors: Bozja
authors: Martin L
authors: ML
authors: Moore
authors: Richard W
authors: RW
authors: Compans
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000276:immunology*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*; D019562:Viral Load / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral; C526850:F protein, human respiratory syncytial virus; C502275:G glycoprotein, Respiratory syncytial virus; D007074:Immunoglobulin G; C488936:M1 protein, Influenza A virus; D022261:Respiratory Syncytial Virus Vaccines; D058425:Vaccines, Virus-Like Particle; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins