title: Two chicken neuromedin U receptors: characterization of primary structure, biological activity and tissue distribution.
abstract: Neuromedin U (NMU) is a bioactive peptide that is involved in a variety of physiological functions. Two of its receptors, NMUR1 and NMUR2, have been identified and characterized in mammals. In this study, we performed cDNA cloning of chicken NMUR1 and NMUR2, and characterized their primary structure, biological activity, and expression patterns in chicken tissues. The chicken NMUR1 and NMUR2 cDNAs encoded 438 and 395 amino acid sequences, respectively. Chicken NMUR1 showed 54.8%-56.5% sequence identity with human, rat, and mouse NMUR1, and NMUR2 shared 67.3%-70.1% sequence identity with mammalian orthologs. Both chicken receptors have typical characteristics of G-protein-coupled receptors with seven transmembrane domains and the D/ERY motif. An increase in intracellular Ca(2+) mobilization was observed in HEK293 cells transfected with chicken NMUR1 or NMUR2 cDNA and treated with chicken or rat NMU. Real-time PCR analysis revealed that NMUR1 mRNA was preferentially expressed in the intestinal tissues such as the duodenum, jejunum, ileum, cecum, and colon/rectum, and brain regions such as the midbrain and optic lobe, and the ovary in adult hens. NMUR2 mRNA was exclusively expressed in the brain regions such as the cerebrum and midbrain. These results indicate that NMUR1 and NMUR2 mRNAs, which encode functional receptor proteins, are expressed in chicken tissues with different distribution patterns.
authors: Department of Veterinary Science, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
authors: Ichiro
authors: I
authors: Yamamoto
authors: Nobuhiro
authors: N
authors: Nakao
authors: Hiroyuki
authors: H
authors: Kaiya
authors: Mikiya
authors: M
authors: Miyazato
authors: Nobumichi
authors: N
authors: Tsushima
authors: Toshiro
authors: T
authors: Arai
authors: Minoru
authors: M
authors: Tanaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002432:Cecum / Q000378:metabolism; D002645:Chickens; D003106:Colon / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D005260:Female; D007082:Ileum / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D017981:Receptors, Neurotransmitter / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D017981:Receptors, Neurotransmitter; C082444:neuromedin U receptor