title: MART-1 is a reliable melanocytic marker in lichen planus-like keratosis: a study on 70 cases.
abstract: Recent studies have proposed that MART-1 may falsely stain clusters of intraepidermal nonmelanocytic cells in lichenoid dermatitides. This may become an issue especially in isolated lesions of lichen planus-like keratosis (LPLK), a condition also known as benign lichenoid keratosis, and one that is often mistaken clinically for a malignant cutaneous neoplasm. LPLKs are known to exhibit basal epidermal pseudonests, mimicking a regressing melanocytic lesion histologically, and often may prompt the pathologist to obtain a MART-1 stain. If MART-1 is falsely positive, it may seal an incorrect diagnosis. To determine whether or not pseudonests in LPLK decorated with MART-1, we reviewed 70 cases from our institution, stained them with MART-1 (Thermo Fisher-Lab Vision, Ab3 clone, 1:400 dilution, heat-induced epitope retrieval with 0.02M citrate buffer at pH 6.0), and evaluated them for the presence or absence of staining within pseudonests. Four cases demonstrated an occasional MART-1-positive junctional nest. In these cases, microphthalmia transcription factor was also positive, confirming a true melanocytic origin. None of the other cases showed a MART-1 pattern that would have been suspicious for a melanocytic lesion. We propose that this discrepancy between our study and prior ones may be explained by differences in staining protocols or by a very low incidence of non-specific staining. Our study suggests that MART-1 is a useful marker in differentiating melanocytic nests from pseudonests in LPLK.
authors: Division of Dermatopathology, Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL 35249, USA.
authors: Alde Carlo P
authors: AC
authors: Gavino
authors: Matthew T
authors: MT
authors: Woods
authors: Aleodor A
authors: AA
authors: Andea
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D003937:Diagnosis, Differential; D006801:Humans; D007150:Immunohistochemistry; D007642:Keratosis / Q000175:diagnosis*; D017512:Lichenoid Eruptions / Q000175:diagnosis*; D058965:MART-1 Antigen; D012878:Skin Neoplasms / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D058965:MART-1 Antigen