title: Calcareous impact on arbuscular mycorrhizal fungus development and on lipid peroxidation in monoxenic roots.
abstract: The present work underlined the negative effects of increasing CaCO(3) concentrations (5, 10 and 20 mM) both on the chicory root growth and the arbuscular mycorrhizal fungus (AMF) Glomus irregulare development in monoxenic system. CaCO(3) was found to reduce drastically the main stages of G. irregulare life cycle (spore germination, germinative hyphae elongation, root colonization, extraradical hyphae development and sporulation) but not to inhibit it completely. The root colonization drop was confirmed by the decrease in the arbuscular mycorrhizal fungal marker C16:1ω5 amounts in the mycorrhizal chicory roots grown in the presence of CaCO(3). Oxidative damage evaluated by lipid peroxidation increase measured by (i) malondialdehyde (MDA) production and (ii) the antioxidant enzyme peroxidase (POD) activities, was highlighted in chicory roots grown in the presence of CaCO(3). However, MDA formation was significantly higher in non-mycorrhizal roots as compared to mycorrhizal ones. This study pointed out the ability of arbuscular mycorrhizal symbiosis to enhance plant tolerance to high levels of CaCO(3) by preventing lipid peroxidation and so less cell membrane damage.
authors: Unité Cultures Maraîchères et Florales (UCMF), Institut National Agronomique de Tunisie (INAT), Mahrajène-Tunis, Tunisia.
authors: Sonia
authors: S
authors: Labidi
authors: Maryline
authors: M
authors: Calonne
authors: Fayçal
authors: F
authors: Ben Jeddi
authors: Djouher
authors: D
authors: Debiane
authors: Salah
authors: S
authors: Rezgui
authors: Frédéric
authors: F
authors: Laruelle
authors: Benoit
authors: B
authors: Tisserant
authors: Anne
authors: A
authors: Grandmougin-Ferjani
authors: Anissa Lounès-Hadj
authors: AL
authors: Sahraoui
mesh_terms: D002119:Calcium Carbonate / Q000494:pharmacology*; D018651:Cichorium intybus / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005227:Fatty Acids / Q000378:metabolism; D055137:Glomeromycota / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D015227:Lipid Peroxidation; D038821:Mycorrhizae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D013312:Stress, Physiological
chemical_list: D005227:Fatty Acids; D002119:Calcium Carbonate