title: The anti-progestin RU-486 inhibits viability of MCF-7 breast cancer cells by suppressing WNT1.
abstract: The Wnt signaling pathway is activated in over 50% of women with breast cancer and contributes to tumor progression. Here, we investigated the effects of RU-486 on Wnt signaling in breast cancer cell lines. RU-486 reduced viability of the progesterone receptor-positive MCF-7 and T-47D cells, but had no effect on the triple-negative MDA-MB-231 cells. Furthermore, RU-486 suppressed WNT1 expression of MCF-7 cells by 99%. The addition of recombinant WNT1 partially reversed the RU-486-dependent inhibition of viability in MCF-7, but not in T-47D cells. In conclusion, we identified WNT1 as a novel mediator of the anti-tumor effects of RU-486 in MCF-7 cells.
authors: Division of Endocrinology, Diabetes, and Metabolic Bone Diseases, Department of Medicine III, Dresden Technical University Medical Center, Germany.
authors: Peggy
authors: P
authors: Benad
authors: Martina
authors: M
authors: Rauner
authors: Tilman D
authors: TD
authors: Rachner
authors: Lorenz C
authors: LC
authors: Hofbauer
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology*; D011980:Receptors, Progesterone / Q000096:biosynthesis; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051155:Wnt1 Protein / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D011980:Receptors, Progesterone; C495247:WNT1 protein, human; D051155:Wnt1 Protein; D015735:Mifepristone