title: Amplitude-encoded calcium oscillations in fish cells.
abstract: The reaction of intracellular Ca(2+) to different agonist stimuli in primary hepatocytes from rainbow trout (Oncorhynchus mykiss) as well as the permanent fish cell line RTL-W1 was investigated systematically. In addition to "classical" agonists such as phenylephrine and ATP, model environmental toxicants like 4-nitrophenol and 3,4-dichloroaniline were used to elucidate possible interactions between toxic effects and Ca(2+) signaling. We report Ca(2+) oscillations in response to several stimuli in RTL-W1 cells and to a lesser extent in primary hepatocytes. Moreover, these Ca(2+) oscillations are amplitude-encoded in contrast to their mammalian counterpart. Bioinformatics and computational analysis were employed to identify key players of Ca(2+) signaling in fish and to determine likely causes for the experimentally observed differences between the Ca(2+) dynamics in fish cells compared to those in mammalian liver cells.
authors: Aquatic Ecology and Toxicology Group, Center of Organismic Studies, University of Heidelberg, Im Neuenheimer Feld 504, D-69120 Heidelberg, Germany. nadja.seitz@zoo.uni-heidelberg.de
authors: N
authors: N
authors: Schweizer
authors: U
authors: U
authors: Kummer
authors: H
authors: H
authors: Hercht
authors: T
authors: T
authors: Braunbeck
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000819:agonists / Q000378:metabolism*; D020013:Calcium Signaling; D002460:Cell Line; D002478:Cells, Cultured; D004785:Environmental Pollutants / Q000378:metabolism; D022781:Hepatocytes / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism; D017683:Oncorhynchus / Q000378:metabolism*; D010656:Phenylephrine / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D004785:Environmental Pollutants; D009596:Nitrophenols; D010656:Phenylephrine; C014464:3,4-dichloroaniline; D000255:Adenosine Triphosphate; D002118:Calcium; C024836:4-nitrophenol