title: Expression and activity of glutathione S-transferases and catalase in the shrimp Litopenaeus vannamei inoculated with a toxic Microcystis aeruginosa strain.
abstract: Microcystin (MC) produced during cyanobacteria blooms is notably toxic to human and wildlife. Conjugation with reduced glutathione (GSH) by glutathione S-transferase (GST) and the antioxidant enzymes defenses (e.g. catalase, CAT) are important biochemical defense mechanisms against MCs toxicity. We investigated the enzymatic activity of CAT and GST and the gene expression levels of CAT and eight GST isoforms in the hepatopancreas of the globally farmed shrimp Litopenaeus vannamei 48-h after injection with a sub-lethal dose of 100 μg kg⁻¹ of a toxic Microcystis aeruginosa extract. MCs caused up-regulation for GSTΩ, μ and a MAPEG isoform, by 12-, 2.8- and 1.8-fold, respectively, and increases in the total GST enzyme activity and CAT enzyme activity. The study points to the importance of further characterization for the L. vannamei GST isoforms and GST/CAT post-translational regulation processes to better understand the key mechanisms involved in the shrimp's defense against MC exposure.
authors: Departamento de Bioquímica, Núcleo de Estudos em Patologia Aquícola, Universidade Federal de Santa Catarina, Servidão Caminho do Porto, s/n, Itacorubi, Florianópolis, SC 88034-257, Brazil.
authors: Daniela
authors: D
authors: Gonçalves-Soares
authors: Juliano
authors: J
authors: Zanette
authors: João S
authors: JS
authors: Yunes
authors: Gloria M
authors: GM
authors: Yepiz-Plascencia
authors: Afonso C D
authors: AC
authors: Bainy
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D001483:Base Sequence; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects / Q000201:enzymology; D008387:Marine Toxins / Q000633:toxicity; D052998:Microcystins / Q000633:toxicity*; D046931:Microcystis; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D001427:Bacterial Toxins; D008387:Marine Toxins; D052998:Microcystins; D020033:Protein Isoforms; D014874:Water Pollutants, Chemical; D002374:Catalase; D005982:Glutathione Transferase; C057862:cyanoginosin LR