title: Isolation and in vitro expansion of human colonic stem cells.
abstract: Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.
authors: Oncology program, Institute for Research in Biomedicine, Barcelona, Spain.
authors: Peter
authors: P
authors: Jung
authors: Toshiro
authors: T
authors: Sato
authors: Anna
authors: A
authors: Merlos-Suárez
authors: Francisco M
authors: FM
authors: Barriga
authors: Mar
authors: M
authors: Iglesias
authors: David
authors: D
authors: Rossell
authors: Herbert
authors: H
authors: Auer
authors: Mercedes
authors: M
authors: Gallardo
authors: Maria A
authors: MA
authors: Blasco
authors: Elena
authors: E
authors: Sancho
authors: Hans
authors: H
authors: Clevers
authors: Eduard
authors: E
authors: Batlle
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D003106:Colon / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology*; D018613:Microscopy, Confocal; D039902:Multipotent Stem Cells / Q000166:cytology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D036183:Receptor, EphB2 / Q000378:metabolism*; D016615:Telomere / Q000378:metabolism
chemical_list: D036183:Receptor, EphB2