title: Quercetin-induced apoptosis involves increased hTERT enzyme activity of leukemic cells.
abstract: We aimed to examine the growth suppressive effects of quercetin on acute promyelocytic and lymphoblastic leukemia and chronic myeloid leukemia, and to find out whether the growth suppression is related to the blocking of telomerase enzyme activity. Cytotoxic effects of quercetin were shown by trypan blue analyses. Apoptotic effects of quercetin were examined by acridine orange and ethidium bromide staining by fluorescence microscopy. The effects of quercetin on telomerase enzyme activity were shown by hTERT Quantification Kit. Our results demonstrated that quercetin has antiproliferative and apoptotic effects on T-cell acute lymphoblastic leukemia (ALL), acute promyelocytic leukemia, and chronic myeloid leukemia (CML) cells. We also showed for the first time by this study that quercetin suppresses the activity of telomerase in ALL and CML cells. The results of this study show the importance of quercetin for its therapeutic potential in treatment of leukemias.
authors: School of Medicine, Ege University, Izmir, Turkey.
authors: Cigir Biray
authors: CB
authors: Avci
authors: Sunde
authors: S
authors: Yilmaz
authors: Zeynep Ozlem
authors: ZO
authors: Dogan
authors: Güray
authors: G
authors: Saydam
authors: Yavuz
authors: Y
authors: Dodurga
authors: Huseyin Atakan
authors: HA
authors: Ekiz
authors: Melis
authors: M
authors: Kartal
authors: Fahri
authors: F
authors: Sahin
authors: Yusuf
authors: Y
authors: Baran
authors: Cumhur
authors: C
authors: Gunduz
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000201:enzymology* / Q000235:genetics; D011794:Quercetin / Q000494:pharmacology* / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D012333:RNA, Messenger; D011794:Quercetin; D019098:Telomerase