title: Diversity of acetic acid bacteria present in healthy grapes from the Canary Islands.
abstract: The identification of acetic acid bacteria (AAB) from sound grapes from the Canary Islands is reported in the present study. No direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from Tenerife, La Palma and Lanzarote islands. Up to 396 AAB were isolated from those microvinifications and identified by 16S rRNA gene sequencing and phylogenetic analysis. With this method, Acetobacter pasteurianus, Acetobacter tropicalis, Gluconobacter japonicus and Gluconacetobacter saccharivorans were identified. However, no discrimination between the closely related species Acetobacter malorum and Acetobacter cerevisiae was possible. As previously described, 16S-23S rRNA gene internal transcribed spacer (ITS) region phylogenetic analysis was required to classify isolates as one of those species. These two species were the most frequently occurring, accounting for more than 60% of the isolates. For typing the AAB isolates, both the Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR and (GTG)5-PCR techniques gave similar resolution. A total of 60 profiles were identified. Thirteen of these profiles were found in more than one vineyard, and only one profile was found on two different islands (Tenerife and La Palma).
authors: Biotecnologia Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, C/Marcel·lí Domingo s/n. 43007 Tarragona, Spain.
authors: Maria José
authors: MJ
authors: Valera
authors: Federico
authors: F
authors: Laich
authors: Sara S
authors: SS
authors: González
authors: Maria Jesús
authors: MJ
authors: Torija
authors: Estibaliz
authors: E
authors: Mateo
authors: Albert
authors: A
authors: Mas
mesh_terms: D000091:Acetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D044822:Biodiversity; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D041821:Gluconacetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D020576:Gluconobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D027843:Vitis / Q000382:microbiology*
chemical_list: D021903:DNA, Ribosomal Spacer; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S