title: [Effect of BMP-2 on osteogenesis of bone mesenchymal stem cells in rats].
abstract: OBJECTIVE
  To determine the effect of bone morphogenetic protein-2(BMP-2) on osteogenesis of bone mesenchymal stem cells(BMSCs) in rats.
  
  
  METHODS
  The primary culture of rat BMSCs was succeeded and then was identified. The rat BMSCs were infected with the recombinant lentivirus with BMP-2(Lenti-BMP-2). The osteogenic effect of BMP-2 was observed. In addition, adhesive ability of BMP-2-BMSCs was detected through adhesion assay and expression of osteogenic factors OPN,OCN,Col-I and smad was observed by RT-PCR and Western blot. SPSS11.0 software package was used for analysis.
  
  
  RESULTS
  Rat BMSCs were cultured and identified successfully. The osteogenic effect of BMSCs was improved by BMP-2. Lenti-BMP-2 BMSCs adhesive potential enhanced and osteogenic factors were up-regulated.
  
  
  CONCLUSIONS
  BMP-2 may facilitate the osteogenic effect of the rat BMSCs and would provide favourable cell resource for tissue engineering.
authors: Department of Stomatology, Fudan University. Shanghai, China. Sunjian225@hotmail.com
authors: Jian
authors: J
authors: Sun
authors: You-cheng
authors: YC
authors: Yu
authors: Zhang-yu
authors: ZY
authors: Gu
authors: Liang
authors: L
authors: Gu
authors: Wei
authors: W
authors: Bi
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D001854:Bone Marrow Cells; D055396:Bone Morphogenetic Protein 2; D002454:Cell Differentiation; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D051381:Rats
chemical_list: D055396:Bone Morphogenetic Protein 2; D000469:Alkaline Phosphatase