title: Detection and characterization of a distinct bornavirus lineage from healthy Canada geese (Branta canadensis).
abstract: Avian bornaviruses (ABV), identified in 2008, infect captive parrots and macaws worldwide. The natural reservoirs of these viruses are unknown. Reverse transcription-PCR (RT-PCR) was used to screen oropharyngeal/cloacal swab and brain samples from wild Canada geese (Branta canadensis) for ABV. Approximately 2.9% of swab samples were positive for bornavirus sequences. Fifty-two percent of brain samples from 2 urban flocks also tested positive, and brain isolates were cultured in duck embryo fibroblasts. Phylogenetic analyses placed goose isolates in an independent cluster, and more notably, important regulatory sequences present in Borna disease virus but lacking in psittacine ABVs were present in goose isolates.
authors: Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, MS#4467, Texas A&M University, College Station, TX 77843, USA. spayne@cvm.tamu.edu
authors: Susan
authors: S
authors: Payne
authors: Lina
authors: L
authors: Covaleda
authors: Guo
authors: G
authors: Jianhua
authors: Seth
authors: S
authors: Swafford
authors: John
authors: J
authors: Baroch
authors: Pamela J
authors: PJ
authors: Ferro
authors: Blanca
authors: B
authors: Lupiani
authors: Jill
authors: J
authors: Heatley
authors: Ian
authors: I
authors: Tizard
mesh_terms: D000818:Animals; D046090:Anseriformes / Q000821:virology*; D028981:Bornaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001921:Brain / Q000821:virology; D002170:Canada; D002460:Cell Line; D002988:Cloaca / Q000821:virology; D016000:Cluster Analysis; D008969:Molecular Sequence Data; D009960:Oropharynx / Q000821:virology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral