title: Molecular cloning, functional characterization and phylogenetic analysis of TRAIL in Japanese pufferfish Takifugu rubripes.
abstract: In this study, the complementary DNA (cDNA) of Japanese pufferfish Takifugu rubripes tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) was cloned by reverse-transcription PCR. The open reading frame of the TRAIL consisted of 870 bases. The deduced amino-acid sequence of the TRAIL showed a high homology with the sequences of other teleosts. Recombinant soluble TRAIL was fused with a small ubiquitin-related modifier gene to enhance the soluble expression level in Escherichia coli BL21 (DE3). In vitro, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrasodium bromide (MTT) assay indicated that the purified soluble TRAIL was able to induce apoptosis of Jurkat and HeLa cells in a dose-dependent manner.
authors: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Science College, Nanjing Normal University, Nanjing, 210046 Jiangsu, People's Republic of China.
authors: J F
authors: JF
authors: Li
authors: H X
authors: HX
authors: Ai
authors: J
authors: J
authors: Zhang
authors: M X
authors: MX
authors: Du
authors: Z
authors: Z
authors: Zhang
authors: J X
authors: JX
authors: Zhang
authors: S Q
authors: SQ
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D040461:Gene Components; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D023741:Takifugu / Q000235:genetics* / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D012333:RNA, Messenger; D053221:TNF-Related Apoptosis-Inducing Ligand