title: [The production of high-yielding strain of rubella virus from wild type virus extracted from a patient in the Western Siberia in 2006].
abstract: The study was targeted to investigate the propagation of rubella virus in the cell cultures of various origins and with different cultivation methods. The high-yielding strain of rubella virus was produced. The "spinner-culture" cultivation method was applied and the strain's RNA was detected in 10-8 dilution in real time mode. This strain is supposed to be used in preparation of the standard antigen to implement in the development of immune enzyme test system targeted to the rubella virus specific antibodies.
authors: G I
authors: GI
authors: Tiunnikov
authors: V S
authors: VS
authors: Petrov
authors: V A
authors: VA
authors: Manuĭlov
authors: S V
authors: SV
authors: Seregin
authors: S V
authors: SV
authors: Netesov
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D002522:Chlorocebus aethiops; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000302:isolation & purification*; D012409:Rubella / Q000175:diagnosis / Q000821:virology; D012412:Rubella virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D012800:Siberia; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral; C040901:rubella antibodies