title: Single-molecule recognition force spectroscopy of transmembrane transporters on living cells.
abstract: Atomic force microscopy (AFM) has proven to be a powerful tool in biological sciences. Its particular advantage over other high-resolution methods commonly used is that biomolecules can be investigated not only under physiological conditions but also while they perform their biological functions. Single-molecule force spectroscopy with AFM tip-modification techniques can provide insight into intermolecular forces between individual ligand-receptor pairs of biological systems. Here we present protocols for force spectroscopy of living cells, including cell sample preparation, tip chemistry, step-by-step AFM imaging, force spectroscopy and data analysis. We also delineate critical steps and describe limitations that we have experienced. The entire protocol can be completed in 12 h. The model studies discussed here demonstrate the power of AFM for studying transmembrane transporters at the single-molecule level.
authors: Institute for Biophysics, Johannes Kepler University of Linz, Linz, Austria.
authors: Theeraporn
authors: T
authors: Puntheeranurak
authors: Isabel
authors: I
authors: Neundlinger
authors: Rolf K H
authors: RK
authors: Kinne
authors: Peter
authors: P
authors: Hinterdorfer
mesh_terms: D000818:Animals; D001665:Binding Sites; D018929:Cell Culture Techniques; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D026901:Membrane Transport Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods*; D051269:Sodium-Glucose Transporter 1 / Q000737:chemistry
chemical_list: D026901:Membrane Transport Proteins; D051269:Sodium-Glucose Transporter 1