title: Comparison of plasmid-mediated quinolone resistance and extended-spectrum β-lactamases in third-generation cephalosporin-resistant Enterobacteriaceae from four Irish hospitals.
abstract: In this study, the frequency of extended-spectrum β-lactamases (ESBL) and plasmid-mediated quinolone resistance (PMQR) mechanisms were investigated in 206 clinical isolates of third-generation cephalosporin (3GC)-resistant Enterobacteriaceae in four hospitals in the Republic of Ireland. bla(CTX-M-15) was the predominant ESBL gene. Of these 3GC resistant isolates, 54 % were also resistant to ciprofloxacin. Investigation of the PMQR mechanisms revealed that the aac(6')Ib-cr gene predominated in fluoroquinolone-resistant (FQR) strains of Escherichia coli and Klebsiella pneumoniae, while the qnrA gene predominated in the FQR strains of Enterobacter. The bla(CTX-M-15) gene was frequently identified with the aac(6')Ib-cr gene but was not always on the same plasmid. The prevalence of the bla(CTX-M-15) gene appeared to be hospital-dependent. The epidemiology of both ESBL-producing and PMQR strains within the four hospitals indicated that their prevalence is not due to the spread of these resistance genes between isolates from different hospitals.
authors: Department of Clinical Microbiology, Sir Patrick Dun Laboratory, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland.
authors: Fiona
authors: F
authors: Walsh
authors: Department of Clinical Microbiology, Sir Patrick Dun Laboratory, University of Dublin, Trinity College, St James's Hospital Campus, Dublin 8, Ireland.
authors: Thomas R
authors: TR
authors: Rogers
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D002511:Cephalosporins / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D006761:Hospitals / Q000706:statistics & numerical data*; D006801:Humans; D007494:Ireland / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D015995:Prevalence; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002511:Cephalosporins; D015363:Quinolones; D001618:beta-Lactamases