title: Transcription specificity of the class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex and comparison with class Ia genes.
abstract: Our aim was to analyse the transcription levels of the three non-classical class Ib genes SLA-6, SLA-7 and SLA-8 of the swine major histocompatibility complex in various tissues and conditions and to compare them to the transcription levels of classical class Ia genes. Twenty-five adult tissues from two pig breeds, pig renal PK15 cells infected with the Pseudorabies virus, and peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide or a mixture of phorbol myristate acetate and ionomycin were included in our study. Relative transcription was quantified by quantitative real-time PCR. On average, in adult tissues and PBMCs and compared to SLA-6, the transcription level of SLA-Ia genes was 100-1000 times higher, the level of SLA-8 was 10-20 times higher, and that of SLA-7 was five times higher. Thus, SLA-8 is the most transcribed SLA-Ib gene, followed by the SLA-7 and SLA-6 genes. The highest transcription levels of SLA-Ib transcripts were found in the lymphoid organs, followed by the lung and the digestive tract. The tissue variability of expression levels was widest for the SLA-6 gene, with a 1:32 ratio between the lowest and highest levels in contrast to a 1:12 ratio for the SLA-7 and SLA-8 genes and a 1:16 ratio for the SLA-Ia genes. During PK-15 infection and PBMC stimulation, SLA-Ia and SLA-8 genes were downregulated, whereas SLA-6 and SLA-7 were upregulated, downregulated or not significantly modified. Our overall results confirm the tissue-wide transcription of the three SLA-Ib genes and suggest that they have complementary roles.
authors: Institute of Animal Science, University Debrecen, Centre for Agricultural and Applied Economic Sciences, 4032 Debrecen, Böszörményi Str.138, Hungary.
authors: S
authors: S
authors: Kusza
authors: L
authors: L
authors: Flori
authors: Y
authors: Y
authors: Gao
authors: A
authors: A
authors: Teillaud
authors: R
authors: R
authors: Hu
authors: G
authors: G
authors: Lemonnier
authors: Z
authors: Z
authors: Bosze
authors: E
authors: E
authors: Bourneuf
authors: S
authors: S
authors: Vincent-Naulleau
authors: C
authors: C
authors: Rogel-Gaillard
mesh_terms: D000818:Animals; D015870:Gene Expression; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009928:Organ Specificity; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D015395:Histocompatibility Antigens Class I