title: [Inhibition of T cell proliferation and T cell-induced osteoclastogenesis by peroxisome proliferator activated receptor-γ ligand].
abstract: OBJECTIVE
  To examine the effect of peroxisome proliferator activated receptor-γ(PPARγ) ligand, 15d-PGJ2 on inhibition of T cell proliferation and T cell-mediated osteoclastogenesis, and clarify the possible mechanism.
  
  
  METHODS
  T cells from A.actinomycetetemcomitans immunized BALB/c mice were isolated from cervical lymph nodes and T lymphocytes enriched in vitro. At 3-day after 15d-PGJ2 (0,1×10(-8),1×10(-7),1×10(-6),1×10(-5)mol/L) induction,T cell proliferation analysis was measured by cintilography, the concentration of RANKL, TNF-α and IL-10 were measured in culture supernatants by ELISA, and osteoclastogenesis of co-culture with RAW 264.7 cell and T lymphocyte supernatants that enriched in vitro by tartrate resistant acid phosphatase (TRAP) staining. SASS 11.0 software package was used for statistical analysis.
  
  
  RESULTS
  By 1×10-5mol/L 15d-PGJ2 induction, T cell 3H incorporation remarkably decreased, the expression of RANKL and TNF-α in the supernatant markedly decreased, significantly different compared with the control group(P<0.05), but IL-10 was not significantly different, and the numbers of TRAP positive cells decreased, significantly different compared with the control group (P<0.05),which was dose-dependent.
  
  
  CONCLUSIONS
  PPARγ ligand 15d-PGJ2 can inhibit 3H incorporation, down-regulate cytokine release, decrease T cell-induced osteoclastogenesis. The data suggest that PPARγ ligand plays an important role in T cell-induced bone resorption.
authors: Department of Stomatology, Shengjing Hospital of China Medical University. Shenyang, Liaoning Province, China. Xiaoping_ba@yahoo.com
authors: Xiao-ping
authors: XP
authors: Lin
authors: Hong-li
authors: HL
authors: Liu
authors: Li-li
authors: LL
authors: DU
authors: Kawai
authors: K
authors: Toshihisa
authors: Chun-hui
authors: CH
authors: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D008024:Ligands; D051379:Mice; D047495:PPAR gamma; D015230:Prostaglandin D2 / Q000031:analogs & derivatives; D053245:RANK Ligand; D013601:T-Lymphocytes; D014409:Tumor Necrosis Factor-alpha
chemical_list: C477819:15-deoxyprostaglandin J2; D008024:Ligands; D047495:PPAR gamma; D053245:RANK Ligand; D014409:Tumor Necrosis Factor-alpha; D015230:Prostaglandin D2