title: Tcf-1 gene silence suppresses downstream gene expression in CD4(+) T cells from bone marrow of aplastic anemia patients.
abstract: CD4(+) T cells play a crucial role in the pathogenesis of acquired aplastic anemia (AA). Tcf-1 gene regulates T cell development and function, and it is significantly upregulated in the bone marrow CD4(+) T cells from patients with acquired AA. To explore the role of Tcf-1 in the pathogenesis of AA, we knocked down Tcf-1 gene in CD4(+) T cells of AA patients and studied the effects of Tcf-1 silencing on its downstream gene expression. Upon transfection of psiRNA into marrow CD4(+) T cells from bone marrow of aplastic anemia patients, the expression of Tcf-1 was significantly knocked down; consequently, expressions of c-Myc and CD44 were also significantly reduced. Our results suggest that Tcf-1 may contribute to pathogenesis of AA by regulating downstream gene expression such as c-myc and CD44.
authors: Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
authors: Miao
authors: M
authors: Zheng
authors: Kai
authors: K
authors: Zheng
authors: Jianfeng
authors: J
authors: Zhou
authors: Hanying
authors: H
authors: Sun
authors: Lifang
authors: L
authors: Huang
authors: Wenli
authors: W
authors: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000503:physiopathology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051822:T Cell Transcription Factor 1 / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
chemical_list: C497483:CD44 protein, human; D018960:Hyaluronan Receptors; C489427:MYC protein, human; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D051822:T Cell Transcription Factor 1; C497576:TCF7 protein, human