title: Haemophilus influenzae protein E binds to the extracellular matrix by concurrently interacting with laminin and vitronectin.
abstract: Nontypeable Haemophilus influenzae (NTHi) causes otitis media and is commonly found in patients with chronic obstructive pulmonary disease (COPD). Adhesins are important for bacterial attachment and colonization. Protein E (PE) is a recently characterized ubiquitous 16 kDa adhesin with vitronectin-binding capacity that results in increased survival in serum. In addition to PE, NTHi utilizes Haemophilus adhesion protein (Hap) that binds to the basement-membrane glycoprotein laminin. We show that most clinical isolates bind laminin and that both Hap and PE are crucial for the NTHi-dependent interaction with laminin as revealed with different mutants. The laminin-binding region is located at the N-terminus of PE, and PE binds to the heparin-binding C-terminal globular domain of laminin. PE simultaneously attracts vitronectin and laminin at separate binding sites, proving the multifunctional nature of the adhesin. This previously unknown PE-dependent interaction with laminin may contribute to NTHi colonization, particularly in smokers with COPD.
authors: Medical Microbiology, Department of Laboratory Medicine Malmö, Lund University, Skåne University Hospital, Malmö, Sweden.
authors: Teresia
authors: T
authors: Hallström
authors: Birendra
authors: B
authors: Singh
authors: Fredrik
authors: F
authors: Resman
authors: Anna M
authors: AM
authors: Blom
authors: Matthias
authors: M
authors: Mörgelin
authors: Kristian
authors: K
authors: Riesbeck
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000502:physiology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001665:Binding Sites; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000737:chemistry*; D005260:Female; D005340:Fibrinogen / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry; D006193:Haemophilus influenzae / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007223:Infant; D007797:Laminin / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D020349:Surface Plasmon Resonance; D019096:Vitronectin / Q000737:chemistry*; D055815:Young Adult
chemical_list: D018829:Adhesins, Bacterial; D005353:Fibronectins; D007797:Laminin; D019096:Vitronectin; D005340:Fibrinogen