title: An acyloxymethyl ketone-based probe to monitor the activity of glutathionylspermidine amidase in Escherichia coli.
abstract: Cellular redox conditions affect Gsp amidase activity in Escherichia coli. Guided by the structure and catalytic mechanism of the amidase, we designed and synthesized an acyloxymethyl ketone-based activity probe containing a biotin handle. This probe was used to monitor Gsp amidase activity in E. coli lysates that had been subjected to oxidative or methylglyoxal-induced stress.
authors: Institute of Biological Chemistry, Academia Sinica, No. 128 Academia Road Section 2, Nan-Kang, Taipei 11529, Taiwan.
authors: Cheong-Meng
authors: CM
authors: Chong
authors: Shijay
authors: S
authors: Gao
authors: Bing-Yu
authors: BY
authors: Chiang
authors: Wen-Hung
authors: WH
authors: Hsu
authors: Ting-Chien
authors: TC
authors: Lin
authors: Tzu-Chieh
authors: TC
authors: Chen
authors: Chun-Hung
authors: CH
authors: Lin
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D001665:Binding Sites; D057075:Enzyme Assays / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010084:Oxidation-Reduction; D013095:Spermidine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D007659:Ketones; C012333:glutathionylspermidine; D000581:Amidohydrolases; C019665:amidase; D005978:Glutathione; D013095:Spermidine