title: Effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against Porphyromonas gingivalis and Enterococcus faecalis.
abstract: BACKGROUND
  Intracanal medication is important for endodontic treatment success as it eliminates microorganisms that persist after biomechanical preparation. Aim.  To evaluate the effect of two intracanal medications against Porphyromonas gingivalis and Enterococcus faecalis in the root canals of human primary teeth with necrotic pulp with and without furcal/periapical lesion, using quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  METHODS
  Thirty-two teeth with necrotic pulp were used. Twelve teeth did not present lesion, and 20 teeth presented radiographically visible furca/periapical lesion. Microbiological samples were collected after coronal access and biomechanical preparation. The teeth were medicated with calcium hydroxide pastes prepared with either polyethylene glycol or chlorhexidine. After 30days, the medication was removed and a third collection was performed. Microbiological samples were processed using qRT-PCR. Data were analysed by Wilcoxon and Mann-Whitney tests (α=0.05).
  
  
  RESULTS
  There was no significant difference in the microbiota present in the primary teeth with and without furcal/periapical lesion. Biomechanical preparation was effective in reducing the number of microorganisms (P<0.05). The intracanal medications had similar antibacterial activity.
  
  
  CONCLUSIONS
  The association of chlorhexidine with calcium hydroxide did not increase the antibacterial activity of the intracanal medication in the treatment of primary teeth with necrotic pulp with and without furcal/periapical lesion.
authors: Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, UNESP - Universidade Estadual Paulista, Araraquara, SP, Brazil. jujugondim@yahoo.com.br
authors: Juliana O
authors: JO
authors: Gondim
authors: Juliana S
authors: JS
authors: Avaca-Crusca
authors: Sandro R
authors: SR
authors: Valentini
authors: Cleslei F
authors: CF
authors: Zanelli
authors: Denise M P
authors: DM
authors: Spolidorio
authors: Elisa M A
authors: EM
authors: Giro
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage; D002126:Calcium Hydroxide / Q000008:administration & dosage*; D002648:Child; D002675:Child, Preschool; D002710:Chlorhexidine / Q000008:administration & dosage*; D015169:Colony Count, Microbial; D019140:Dental Care for Children / Q000379:methods; D003786:Dental Pulp Cavity / Q000382:microbiology; D003790:Dental Pulp Necrosis / Q000150:complications / Q000382:microbiology / Q000628:therapy*; D004311:Double-Blind Method; D004338:Drug Combinations; D013293:Enterococcus faecalis / Q000187:drug effects; D005260:Female; D017823:Furcation Defects / Q000150:complications / Q000628:therapy; D006801:Humans; D008297:Male; D009824:Ointments; D010483:Periapical Diseases / Q000150:complications / Q000628:therapy; D011092:Polyethylene Glycols / Q000008:administration & dosage; D016966:Porphyromonas gingivalis / Q000187:drug effects; D012388:Root Canal Irrigants / Q000008:administration & dosage*; D018709:Statistics, Nonparametric; D014094:Tooth, Deciduous / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D000890:Anti-Infective Agents; D004338:Drug Combinations; D009824:Ointments; D012388:Root Canal Irrigants; D011092:Polyethylene Glycols; D002126:Calcium Hydroxide; D002710:Chlorhexidine