title: Pharmacokinetic profile of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside in mice after oral administration of Polygonum multiflorum extract.
abstract: BACKGROUND
  Stilbene glycoside (2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside) is a main bioactive component of Polygonum multiflorum, a traditional Chinese medicine (TCM) commonly used in clinic for anti-aging treatment. Its medicinal activities, such as anti-oxidation, anti-inflammation and endothelial protection, have been extensively studied, but its pharmacokinetic property is still unclear.
  
  
  OBJECTIVE
  A pharmacokinetic study was undertaken to quantitatively determine P. multiflorum stilbene glycoside (PM-SG) in mouse plasma after oral administration of 100 mg/kg P. multiflorum extract.
  
  
  METHODS
  A sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with liquid-liquid phase extraction method was employed for this study. Pharmacokinetic parameters of PM-SG were determined in mice applying both compartmental and non-compartmental analyses.
  
  
  CONCLUSIONS
  The calibration curve for PM-SG in the plasma was linear (r(2) > 0.99) over the range of 0.66 to 56.40 μg/ml, and the concentration-time curve was plotted with the maximum concentration (C(max)) and time to reach maximum concentration (T(max)) of 29.62 μg/ml and 60 min, respectively. The intra- and inter-day variations were less than 3% for relative standard deviation (RSD) and relative error (RE), with a good recovery of more than 97% (RSD <3%). All pharmacokinetic parameters estimated by compartmental and non-compartmental models reached a same conclusion that PM-SG was rapidly absorbed and widely distributed throughout the body with a great efficiency of utility, followed by quick elimination and clearance.
  
  
  CONCLUSIONS
  This was the first report on determination of the pharmacokinetic profile of PM-SG in mice after oral administration. The result may provide a meaningful basis for evaluating the clinical applications of such a bioactive compound from herbal medicines.
authors: Zhejiang Chinese Medical University, Hangzhou, P.R. China.
authors: Guiyuan
authors: G
authors: Lv
authors: Hui
authors: H
authors: Gu
authors: Suhong
authors: S
authors: Chen
authors: Zhaohuan
authors: Z
authors: Lou
authors: Letian
authors: L
authors: Shan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D023421:Models, Animal; D028321:Plant Preparations / Q000493:pharmacokinetics*; D018517:Plant Roots; D010946:Plants, Medicinal; D028502:Polygonum / Q000737:chemistry; D013267:Stilbenes / Q000493:pharmacokinetics*
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D028321:Plant Preparations; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside