title: A microfluidic in situ analyzer for ATP quantification in ocean environments.
abstract: We have developed and tested a functionally integrated in situ analyzer, the IISA-ATP system, for microbial activity assays based on a quantitative determination of the total (particulate and dissolved) ATP in ocean environments. The IISA-ATP utilizes a PDMS-glass hybrid microfluidic device as its core functional element, which can perform cell lysis and total ATP quantification by a luciferin-luciferase bioluminescence assay in situ. Transparent heaters and a temperature sensor fabricated on a glass substrate provide temperature control. As a result of the evaluation using the microfluidic device with ATP standard solutions, the bioluminescence intensity was linearly correlated with 2 × 10(-12) to 2 × 10(-8) M of ATP. A detection limit of 1.1 × 10(-11) M was determined using the completed IISA-ATP system, which includes a miniature pumping module and a control module. As a result of the evaluation using the environmental seawater sample collected from Tokyo Bay, Japan, 2.7 × 10(-10) M of total ATP was successfully determined in the laboratory by the IISA-ATP. The system was operated at a shallow submarine hot spring area in Okinawa, Japan for an in situ trial. The result shows the system was successfully operated in situ and the total ATP was determined to be 3.4 × 10(-10) M.
authors: Institute of Industrial Science, University of Tokyo, Tokyo, Japan. bafuk@iis.u-tokyo.ac.jp
authors: Tatsuhiro
authors: T
authors: Fukuba
authors: Yusuke
authors: Y
authors: Aoki
authors: Noriyuki
authors: N
authors: Fukuzawa
authors: Takatoki
authors: T
authors: Yamamoto
authors: Masanori
authors: M
authors: Kyo
authors: Teruo
authors: T
authors: Fujii
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D007753:Laboratories; D008163:Luminescent Measurements; D008829:Microbiology / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012623:Seawater / Q000382:microbiology*; D018511:Systems Integration; D013696:Temperature
chemical_list: D000255:Adenosine Triphosphate