title: Molecular characterization and solution properties of enzymatically tailored arabinoxylans.
abstract: Two α-L-arabinofuranosidases with different substrate specificities were used to modify the arabinose-to-xylose ratio of cereal arabinoxylans: one enzyme (AXH-m) removed the L-arabinofuranosyl substituents from the monosubstituted xylopyranosyl residues and the other (AXH-d3) the (1→3)-linked L-arabinofuranosyl units from the disubstituted xylopyranosyl residue. In this study, we noticed that not only the arabinose-to-xylose ratio but also the position of the arabinofuranosyl substituents affects the water-solubility of arabinoxylans. The AXH-d3 treatment had no significant effect on the solution conformation of arabinoxylans, but the density of the arabinoxylan molecules decreased in DMSO solution after AXH-m modification. The possible heterogeneity of arabinoxylans complicated the interpretation of data describing the macromolecular properties of the enzymatically modified samples.
authors: Department of Food and Environmental Sciences, PO Box 27, FIN-00014 University of Helsinki, Finland. leena.m.pitkanen@helsinki.fi
authors: Leena
authors: L
authors: Pitkänen
authors: Päivi
authors: P
authors: Tuomainen
authors: Liisa
authors: L
authors: Virkki
authors: Maija
authors: M
authors: Tenkanen
mesh_terms: D001089:Arabinose / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012434:Secale / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility; D012996:Solutions; D013379:Substrate Specificity; D014908:Triticum / Q000737:chemistry* / Q000378:metabolism; D014990:Xylans / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D007527:Isoenzymes; D012996:Solutions; D014990:Xylans; C085118:arabinoxylan; D014994:Xylose; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase