title: A multifunctional core-shell nanoparticle for dendritic cell-based cancer immunotherapy.
abstract: Dendritic cell-based cancer immunotherapy requires tumour antigens to be delivered efficiently into dendritic cells and their migration to be monitored in vivo. Nanoparticles have been explored as carriers for antigen delivery, but applications have been limited by the toxicity of the solvents used to make nanoparticles, and by the need to use transfection agents to deliver nanoparticles into cells. Here we show that an iron oxide-zinc oxide core-shell nanoparticle can deliver carcinoembryonic antigen into dendritic cells while simultaneously acting as an imaging agent. The nanoparticle-antigen complex is efficiently taken up by dendritic cells within one hour and can be detected in vitro by confocal microscopy and in vivo by magnetic resonance imaging. Mice immunized with dendritic cells containing the nanoparticle-antigen complex showed enhanced tumour antigen specific T-cell responses, delayed tumour growth and better survival than controls.
authors: Department of Microbiology and Immunology, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul, Republic of Korea.
authors: Nam-Hyuk
authors: NH
authors: Cho
authors: Taek-Chin
authors: TC
authors: Cheong
authors: Ji Hyun
authors: JH
authors: Min
authors: Jun Hua
authors: JH
authors: Wu
authors: Sang Jin
authors: SJ
authors: Lee
authors: Daehong
authors: D
authors: Kim
authors: Jae-Seong
authors: JS
authors: Yang
authors: Sanguk
authors: S
authors: Kim
authors: Young Keun
authors: YK
authors: Kim
authors: Seung-Yong
authors: SY
authors: Seong
mesh_terms: D000818:Animals; D002272:Carcinoembryonic Antigen / Q000276:immunology / Q000494:pharmacology*; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D004337:Drug Carriers / Q000494:pharmacology*; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects* / Q000276:immunology; D016219:Immunotherapy, Adoptive / Q000379:methods*; D051379:Mice; D053758:Nanoparticles; D009369:Neoplasms / Q000276:immunology / Q000628:therapy*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D002272:Carcinoembryonic Antigen; D004337:Drug Carriers