title: Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples.
abstract: In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.
authors: Centro de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil, 01246-000. mariliadejoao@yahoo.com.br
authors: João Leandro de Paula
authors: JL
authors: Ferreira
authors: Samanta Etel Treiger
authors: SE
authors: Borborema
authors: Luis Fernando de Macedo
authors: LF
authors: Brígido
authors: Maria Isabel de
authors: MI
authors: Oliveira
authors: Terezinha Maria de
authors: TM
authors: Paiva
authors: Cecília Luiza Simões dos
authors: CL
authors: Santos
mesh_terms: D001938:Brazil / Q000453:epidemiology; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000401:mortality / Q000821:virology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D058873:Pandemics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis
chemical_list: C543345:H1N1 virus hemagglutinin; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral