title: L-type Cav1.2 calcium channel is involved in 6-hydroxydopamine-induced neurotoxicity in rats.
abstract: Evidence suggested that L-type calcium channels may play a key role in the pathogenesis of dopaminergic neuron degeneration. In the present study, effects of L-type Cav1.2 calcium channel on 6-hydroxydopamine (6-OHDA)-induced neurotoxicity were investigated. By the semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) studies, we showed that the expression of L-type Cav1.2 calcium channel α1 subunit mRNA increased in the substantia nigra (SN) of 6-OHDA-lesioned rats. Treatment with nifedipine could improve the apomorphine-induced rotation behavior in 6-OHDA-lesioned rats. Using high-performance liquid chromatography electrochemical detection, we also observed that nifedipine partly restored 6-OHDA-induced dopamine depletion in the striatum of rats. These results suggest that the L-type Cav1.2 calcium channel is associated with the development and progression of dopaminergic neuron degeneration.
authors: Department of Physiology, Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines: Physiology, Medical College of Qingdao University, Qingdao 266071, China.
authors: Rong
authors: R
authors: Wang
authors: Zegang
authors: Z
authors: Ma
authors: Jun
authors: J
authors: Wang
authors: Junxia
authors: J
authors: Xie
mesh_terms: D000818:Animals; D000978:Antiparkinson Agents / Q000494:pharmacology; D001058:Apomorphine / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000235:genetics* / Q000378:metabolism*; D004298:Dopamine / Q000172:deficiency / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000187:drug effects* / Q000473:pathology; D004347:Drug Interactions; D005260:Female; D009543:Nifedipine / Q000494:pharmacology; D016627:Oxidopamine / Q000633:toxicity*; D020734:Parkinsonian Disorders / Q000139:chemically induced* / Q000188:drug therapy / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D013565:Sympatholytics / Q000633:toxicity
chemical_list: D000978:Antiparkinson Agents; C504728:Cacna1c protein, rat; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D012333:RNA, Messenger; D013565:Sympatholytics; D016627:Oxidopamine; D009543:Nifedipine; D001058:Apomorphine; D004298:Dopamine