title: Escherichia coli O115 forms fewer attaching and effacing lesions in the ovine colon in the presence of E. coli O157:H7.
abstract: Escherichia coli O115 has been isolated from healthy sheep and was shown to be associated with attaching-effacing (AE) lesions in the large intestine. Following previous observations of interactions between E. coli O157 and O26, the aim of the present study was to assess what influence an O115 AE E. coli (AEEC) would have on E. coli O157 colonisation in vitro and in vivo. We report that E. coli O115- and O157-associated AE lesions were observed on HEp-2 cells and on the mucosa of ligated ovine spiral colon. In single strain inoculum, E. coli O115 associated intimately with HEp-2 cells and the spiral colon in greater numbers than E. coli O157:H7. However, in mixed inoculum studies, the number of E. coli O115 AE lesions was significantly reduced suggesting negative interference by E. coli O157. Use of the ligated colon model in the present work has allowed in vitro observations to be extended and confirmed whilst using a minimum of experimental animals. The findings support a hypothesis that some AEEC can inhibit adhesion of other AEEC in vivo. The mechanisms involved may prove to be of utility in the control of AE pathovars.
authors: Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (Weybridge), Woodham Lane, Addlestone, Surrey KT15 3NB, UK.
authors: R M
authors: RM
authors: La Ragione
authors: A
authors: A
authors: Best
authors: I
authors: I
authors: Aktan
authors: F A
authors: FA
authors: Clifton-Hadley
authors: A H
authors: AH
authors: Dugdale
authors: U
authors: U
authors: Weyer
authors: T
authors: T
authors: Crawley
authors: P
authors: P
authors: Townsend
authors: W A
authors: WA
authors: Cooley
authors: L
authors: L
authors: Johnson
authors: A
authors: A
authors: Wangoo
authors: M J
authors: MJ
authors: Woodward
authors: A D
authors: AD
authors: Wales
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D003106:Colon / Q000382:microbiology* / Q000473:pathology; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D012756:Sheep / Q000382:microbiology; D012757:Sheep Diseases / Q000382:microbiology* / Q000473:pathology