title: Opioid-like antinociceptive effects of oral administration of a lectin purified from the seeds of Canavalia brasiliensis.
abstract: The objective of this study was to evaluate the antinociceptive effects of a lectin from Canavalia brasiliensis (ConBr) when administered orally to murine models of chemical and thermal nociception. ConBr up to 100 mg/kg produced significant and dose-dependent antinociceptive effects: 81% reduction in abdominal writhing induced by 0.6% acetic acid; 26 and 52% reduction in early- and late-stage paw licking, respectively, induced by 2.5% formalin; and 155% increase in reaction latency (heightened thermal pain threshold). In all models, the antinociceptive effect was reversed by the lectin-binding carbohydrate α-d-methyl-mannoside and by the nonselective opioid antagonist naloxone. The antinociceptive effect observed in the formalin test was inhibited by the δ-selective antagonist naltrindole and the κ-selective antagonist nor-binaltorphimine but not by the μ-selective antagonist cyprodime. In conclusion, when administered orally to Swiss mice, the ConBr lectin displayed antinociceptive activity, both peripheral and central, mediated by the opioid system and involving δ-and κ-receptors and the lectin domain.
authors: Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Av. Paranjana 1700, 60.740-000, Fortaleza, Ceará, Brazil.
authors: Alana
authors: A
authors: de Freitas Pires
authors: Ana Maria Sampaio
authors: AM
authors: Assreuy
authors: Érika Augusta Batista
authors: ÉA
authors: Lopes
authors: Natália Rocha
authors: NR
authors: Celedônio
authors: Carlos Eduardo Alves
authors: CE
authors: Soares
authors: Natália Velloso Fontenelle Camelo
authors: NV
authors: Rodrigues
authors: Paloma Leão
authors: PL
authors: Sousa
authors: Raquel Guimarães
authors: RG
authors: Benevides
authors: Celso Shiniti
authors: CS
authors: Nagano
authors: Benildo Sousa
authors: BS
authors: Cavada
authors: José Henrique
authors: JH
authors: Leal-Cardoso
authors: Andrelina Noronha
authors: AN
authors: Coelho-de-Souza
authors: Cláudia Ferreira
authors: CF
authors: Santos
mesh_terms: D000284:Administration, Oral; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000701:Analgesics, Opioid / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D032449:Canavalia / Q000737:chemistry*; D051379:Mice; D009019:Morphinans / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D059225:Nociception / Q000187:drug effects*; D010147:Pain Measurement / Q000379:methods; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017465:Receptors, Opioid, delta / Q000037:antagonists & inhibitors; D017473:Receptors, Opioid, kappa / Q000037:antagonists & inhibitors; D012639:Seeds / Q000737:chemistry
chemical_list: D000700:Analgesics; D000701:Analgesics, Opioid; D009019:Morphinans; D037121:Plant Lectins; D017465:Receptors, Opioid, delta; D017473:Receptors, Opioid, kappa; C039268:lectin, Canavalia; C058450:cyprodime; D009270:Naloxone; C051844:norbinaltorphimine; D009271:Naltrexone; C055382:naltrindole