title: Nucleotides part LXXX: Synthesis of 3'-O fluorescence labeled thymidine derivatives and their 5'-O-triphosphates.
abstract: A new labeling technique attaching a fluorescent pteridine derivative (3, 5) via a linker onto the 3'-OH group of 5'-O-dimethoxytritylthymidine (7) was developed to lead to the conjugates 8 and 11. After detritylation to give 9 and 12, the final conversion into the corresponding 5'-triphosphates (13, 14), which were isolated as sodium salts, was performed by known methods.
authors: Fachbereich Chemie, Universität Konstanz, Konstanz, Germany.
authors: Tamara I
authors: TI
authors: Prykota
authors: Wolfgang
authors: W
authors: Pfleiderer
mesh_terms: D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D011122:Polyphosphates / Q000138:chemical synthesis* / Q000737:chemistry; D011621:Pteridines / Q000138:chemical synthesis / Q000737:chemistry; D013936:Thymidine / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D011122:Polyphosphates; D011621:Pteridines; C028273:5'-O-dimethyltritylthymidine; C005692:triphosphoric acid; D013936:Thymidine