title: Concise review: Human cell engineering: cellular reprogramming and genome editing.
abstract: Cell engineering is defined here as the collective ability to both reset and edit the genome of a mammalian cell. Until recently, this had been extremely challenging to achieve as nontransformed human cells are significantly refractory to both these processes. The recent success in reprogramming somatic cells into induced pluripotent stem cells that are self-renewable in culture, coupled with our increasing ability to effect precise and predesigned genomic editing, now readily permits cellular changes at both the genetic and epigenetic levels. These dual capabilities also make possible the generation of genetically matched, disease-free stem cells from patients for regenerative medicine. The objective of this review is to summarize the key enabling developments on these two rapidly evolving research fronts in human cell engineering, highlight unresolved issues, and outline potential future research directions.
authors: Stem Cell Program, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors: Prashant
authors: P
authors: Mali
authors: Linzhao
authors: L
authors: Cheng
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D060846:Cell Engineering; D065150:Cellular Reprogramming / Q000235:genetics; D044127:Epigenesis, Genetic / Q000235:genetics; D016678:Genome; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology