title: Effect of oral hepatocyte growth factor gene mediated by attenuated salmonella on 2-, 4-, 6-trinitro-benzene-sulfonic-acid-induced ulcerative colitis in rat.
abstract: OBJECTIVE
  In order to explore a new therapeutic method, we investigated the effects of exogenously expressed hepatocyte growth factor mediated by attenuated salmonella (TPH) on rats with ulcerative colitis (UC) induced by 2-, 4-, 6-trinitro-benzene-sulfonic acid.
  
  
  METHODS
  The UC rats were treated with TPH, attenuated salmonella with a eukaryotic expression vector (TP) or sodium bicarbonate (model control [MC]) every other day. Cluster of differentiation (CD)4(+) and CD8(+) T cells and immunoglobulins in the blood were analyzed by flow cytometry. The HGF expression was determined by immunohistochemistry. A macroscopic-scale observation of the colon and a histological assessment were also carried out.
  
  
  RESULTS
  The CD4(+) T counts and the CD4(+) /CD8(+) ratio in the TPH group were significantly lower than that in the MC group. The immunoglobulin M and immunoglobulin G(1) levels in the TPH group were significantly lower than that in the MC group and TP group. After treatment with TPH, the symptoms of the ulcerative rats were significantly alleviated. The colonic lesion grades in the TPH group were lower than that in the TP group and MC group. Significant improvement occurred after the TPH treatment, as evidenced by alleviated mucosal inflammation. At 7 days post-treatment, the HGF expression in the colonic tissues that were treated with TPH was stronger than that in the samples treated with TP.
  
  
  CONCLUSIONS
  TPH inhibits the proliferation of T lymphocytes and the antibody production of B lymphocytes. Furthermore, it ameliorates mucosal inflammation and promotes the regeneration of mucosa and the healing of the colonic ulceration.
authors: Medical Experiment Center, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China. haxq@yahoo.com
authors: Xiaoqin
authors: X
authors: Ha
authors: Qiang
authors: Q
authors: Yin
authors: Ling
authors: L
authors: Hui
authors: Qinghua
authors: Q
authors: Jia
authors: Meiliang
authors: M
authors: Wang
authors: Junli
authors: J
authors: Zhang
authors: Yuebin
authors: Y
authors: Xu
authors: Yewei
authors: Y
authors: Chang
authors: Xiaopeng
authors: X
authors: Yang
mesh_terms: D000818:Animals; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology*; D005260:Female; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use*; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000235:genetics; D014169:Transformation, Bacterial; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D017228:Hepatocyte Growth Factor; D014302:Trinitrobenzenesulfonic Acid