title: Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green.
abstract: Hepatocytes derived from embryonic stem cells (ESCs) are expected to be useful for basic research and clinical applications. However, in several studies, genetic methods used to detect and obtain them are difficult and pose major safety problems. Therefore, in this study, we established a novel detection system for hepatocytes by using indocyanine green (ICG), which is selectively taken up by hepatocytes, based on nongenetic manipulation. ICG has maximum light absorption near 780 nm, and it fluoresces between 800 and 900 nm. Making use of these properties, we developed flow cytometry equipped with an excitation lazer of 785 nm and specific bandpass filters and successfully detected ESC-derived ICG-positive cells that were periodic acid-Schiff positive and expressed hepatocyte phenotypic mRNAs. These results demonstrate that this detection system based on nongenetic manipulation with ICG will lead to isolate hepatocytes generated from ESCs and provide the appropriate levels of stability, quality, and safety required for cell source for cell-based therapy and pharmaceutical studies such as toxicology.
authors: Department of Histology and Embryology, Shinshu University School of Medicine, Matsumoto, Japan. syoshie@shinshu-u.ac.jp
authors: Susumu
authors: S
authors: Yoshie
authors: Jun
authors: J
authors: Ito
authors: Sakiko
authors: S
authors: Shirasawa
authors: Tadayuki
authors: T
authors: Yokoyama
authors: Yuu
authors: Y
authors: Fujimura
authors: Kazuo
authors: K
authors: Takeda
authors: Masahiro
authors: M
authors: Mizuguchi
authors: Ken
authors: K
authors: Matsumoto
authors: Daihachiro
authors: D
authors: Tomotsune
authors: Katsunori
authors: K
authors: Sasaki
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods; D057809:HEK293 Cells; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D007208:Indocyanine Green / Q000494:pharmacology*; D051379:Mice; D010504:Periodic Acid / Q000494:pharmacology; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004396:Coloring Agents; D010504:Periodic Acid; D007208:Indocyanine Green