title: Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips.
abstract: We present a fast and versatile method to produce functional micro free-flow electrophoresis chips. Microfluidic structures were generated between two glass slides applying multistep liquid-phase lithography, omitting troublesome bonding steps or cost-intensive master structures. Utilizing a novel spacer-less approach with the photodefinable polymer polyethyleneglycol dimethacrylate (PEG-DA), microfluidic devices with hydrophilic channels of only 25 μm in height were generated. The microfluidic chips feature ion-permeable segregation walls between the electrode channels and the separation bed and hydrophilic surfaces. The performance of the chip is demonstrated by free-flow electrophoretic separation of fluorescent xanthene dyes and fluorescently labeled amino acids.
authors: Institut für Analytische Chemie, Universität Leipzig, Linnéstr 3, 04103 Leipzig, Germany.
authors: Stefan
authors: S
authors: Jezierski
authors: Leonid
authors: L
authors: Gitlin
authors: Stefan
authors: S
authors: Nagl
authors: Detlev
authors: D
authors: Belder