title: Accessing the genomic effects of naked nanoceria in murine neuronal cells.
abstract: Cerium oxide nanoparticles (nanoceria) are engineered nanoparticles whose versatility is due to their unique redox properties. We and others have demonstrated that naked nanoceria can act as antioxidants to protect cells against oxidative damage. Although the redox properties may be beneficial, the genome-wide effects of nanoceria on gene transcription and associated biological processes remain elusive. Here we applied a functional genomic approach to examine the genome-wide effects of nanoceria on global gene transcription and cellular functions in mouse neuronal cells. Importantly, we demonstrated that nanoceria induced chemical- and size-specific changes in the murine neuronal cell transcriptome. The nanoceria contributed more than 83% of the population of uniquely altered genes and were associated with a unique spectrum of genes related to neurological disease, cell cycle control, and growth. These observations suggest that an in-depth assessment of potential health effects of naked nanoceria and other naked nanoparticles is both necessary and imminent.
  
  
  BACKGROUND
  Cerium oxide nanoparticles are important antioxidants, with potential applications in neurodegenerative conditions. This team of investigators demonstrated the genomic effects of nanoceria, showing that it induced chemical- and size-specific changes in the murine neuronal cell transcriptome.
authors: Laboratory of Clinical Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors: Tin-Lap
authors: TL
authors: Lee
authors: Joan M
authors: JM
authors: Raitano
authors: Owen M
authors: OM
authors: Rennert
authors: Siu-Wai
authors: SW
authors: Chan
authors: Wai-Yee
authors: WY
authors: Chan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002563:Cerium / Q000737:chemistry / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D046228:Microarray Analysis; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D002563:Cerium; C030583:ceric oxide