title: [Preparation and identification of monoclonal antibodies against Pla protein of Yersinia pestis].
abstract: OBJECTIVE
  To prepare the monoclonal antibody of Pla with recombinant Pla (rPla) by hybridoma cell technology, which will lay the foundation for related research work.
  
  
  METHODS
  Purified rPla was collected by washing repeatedly with urea, and BALB/c mice were immunized by them. Hybridoma cells were achieved by Sp2/0 cell fusion with mouse spleen cells from successfully immunized mice. Monoclonal antibody was screened by indirect ELISA and Western blots with rPla, natural crude Pla and GST respectively.
  
  
  RESULTS
  Three strains of hybridoma cells (named 15B8, 14H4 and 19A4 respectively) which secreted stably the monoclonal antibody of Pla were obtained. Their subclasses were IgG2a and IgG1 in heavy chains and κ chains in light chains. The ELISA titers of ascites were 10(6); respectively.Three of monoclonal antibody can react with natural crude Pla tested by western blots.
  
  
  CONCLUSIONS
  Monoclonal antibody of natural Pla of Yersinia pestis were successfully got, which has laid the foundation for further study of the Pla protein and development diagnosis reagent.
authors: Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China. dch6890728@163.com
authors: Chun-Hong
authors: CH
authors: DU
authors: Peng
authors: P
authors: Wang
authors: Jian-Zhong
authors: JZ
authors: Zhang
authors: Xue
authors: X
authors: Tang
authors: Zhi-Zhong
authors: ZZ
authors: Song
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D045744:Cell Line, Tumor; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010960:Plasminogen Activators / Q000276:immunology*; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007074:Immunoglobulin G; C078140:Pla protease, Yersinia pestis; D010960:Plasminogen Activators