title: Comparative study of fungal cell disruption--scope and limitations of the methods.
abstract: Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, application of chemicals of various types, and osmotic shock. The release of proteins from fungal cells and the activity of a cytosolic enzyme, glucose-6-phosphate dehydrogenase, in the crude extracts were assayed to determine and compare the efficacy of each method. The presented studies allowed adjusting the particular method to a particular strain. The mechanical methods of disintegration appeared to be the most effective for the disintegration of yeast, R. gracilis, and filamentous fungi, A. fumigatus and P. citrinum. Ultrasonication and bead milling led to obtaining fungal cell-free extracts containing high concentrations of soluble proteins and active glucose-6-phosphate dehydrogenase systems.
authors: Department of Bioorganic Chemistry, Wroclaw University of Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland. magdalena.klimek-ochab@pwr.wroc.pl
authors: Magdalena
authors: M
authors: Klimek-Ochab
authors: Małgorzata
authors: M
authors: Brzezińska-Rodak
authors: Ewa
authors: E
authors: Zymańczyk-Duda
authors: Barbara
authors: B
authors: Lejczak
authors: Paweł
authors: P
authors: Kafarski
mesh_terms: D001232:Aspergillus fumigatus / Q000187:drug effects* / Q000201:enzymology; D002457:Cell Extracts / Q000737:chemistry*; D002473:Cell Wall / Q000187:drug effects*; D003902:Detergents / Q000494:pharmacology; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D009997:Osmotic Pressure; D010407:Penicillium / Q000187:drug effects* / Q000201:enzymology; D012248:Rhodotorula / Q000187:drug effects* / Q000201:enzymology; D013010:Sonication
chemical_list: D002457:Cell Extracts; D003902:Detergents; D005656:Fungal Proteins; D005954:Glucosephosphate Dehydrogenase