title: Cloning, expression of a peroxiredoxin gene from Acinetobacter sp. SM04 and characterization of its recombinant protein for zearalenone detoxification.
abstract: Zearalenone (ZEN) is a Fusarium mycotoxin, which has been associated with hyperestrogenism and other reproductive disorders in farm animals. ZEN-contaminated grains as well as its by-products had engendered numerous economic losses to farm animals' production, so the detoxification of ZEN-contaminated grains and its by-products would be necessary and beneficial. In this study, a peroxiredoxin (Prx) gene from Acinetobacter sp. SM04 was cloned, and over-expressed in Escherichia coli BL21 (DE3). The Prx gene of Acinetobacter sp. SM04 encodes a protein of 187 amino acids residues and NCBI BLAST program analysis of deduced amino acids shows high identity with 2-Cys Prx family. Interestingly, recombinant Prx show efficient ability to degrade ZEN using H(2)O(2). Results of MCF-7 cell proliferation assay also found ZEN were oxidized into little estrogenic metabolites by purified recombinant Prx plus H(2)O(2). Further, model experiments on decontamination of ZEN-contaminated corn using recombinant Prx were performed, and results found nearly 90% of ZEN was degraded when crushed ZEN-contaminated corn samples (nearly 1,000 μg ZEN per kg grain) were treated with purified recombinant Prx plus 0.09% (m/m) H(2)O(2) for 6h at 30°C. In addition, the optimum pH and temperature of purified recombinant Prx for ZEN degradation were 9.0 and 70°C respectively.
authors: College of Light Industry and Food, South China University of Technology, Guangzhou 510641, China.
authors: Yuanshan
authors: Y
authors: Yu
authors: Hui
authors: H
authors: Wu
authors: Yuqian
authors: Y
authors: Tang
authors: Liping
authors: L
authors: Qiu
mesh_terms: D000150:Acinetobacter / Q000737:chemistry*; D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D004968:Estrogens, Non-Steroidal / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry; D015870:Gene Expression; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D058751:Livestock; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000378:metabolism; D054464:Peroxiredoxins / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004968:Estrogens, Non-Steroidal; D009183:Mycotoxins; D011994:Recombinant Proteins; D015025:Zearalenone; D006861:Hydrogen Peroxide; D054464:Peroxiredoxins