title: Production and characterization of recombinant transmembrane proteins from Mycoplasma hyopneumoniae.
abstract: Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a chronic respiratory disease which causes significant economic losses to the swine industry worldwide. More efficient strategies for controlling this disease are necessary. In this study, we cloned17 genes coding for transmembrane proteins from M. hyopneumoniae, among which six were successfully expressed in Escherichia coli and had their immunogenic and antigenic properties evaluated. All proteins were immunogenic in mice and sera from naturally infected pigs reacted with the recombinant proteins, suggesting that they are expressed during infection. These antigens may contribute for the development of new recombinant vaccines and diagnostic tests against EP.
authors: Laboratório de Biologia Molecular, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors: S B
authors: SB
authors: Marchioro
authors: S
authors: S
authors: Simionatto
authors: V
authors: V
authors: Galli
authors: F R
authors: FR
authors: Conceição
authors: C B
authors: CB
authors: Brum
authors: A
authors: A
authors: Fisch
authors: C K
authors: CK
authors: Gomes
authors: O A
authors: OA
authors: Dellagostin
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D056724:Immunity, Humoral / Q000276:immunology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D045705:Mycoplasma hyopneumoniae / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic