title: CaMF2, an anther-specific lipid transfer protein (LTP) gene, affects pollen development in Capsicum annuum L.
abstract: Based on the gene differential expression analysis performed by cDNA-amplified fragment length polymorphism (cDNA-AFLP) in the genic male sterile-fertile line 114AB of Capsicum annuum L., a variety of differentially expressed cDNA fragments were detected in fertile or sterile lines. A transcript-derived fragment (TDF) specifically accumulated in the flower buds of fertile line was isolated, and the corresponding full-length cDNA and DNA were subsequently amplified. Bioinformatical analyses of this gene named CaMF2 showed that it encodes a lipid transfer protein with 94 amino acids. Spatial and temporal expression patterns analysis indicated that CaMF2 was an anther-specific gene and the expression of CaMF2 was detected only in flower buds at stage 3-7 of male fertile line with a peak expression at stage 4, but not detected in the roots, tender stems, fresh leaves, flower buds, open flowers, sepals, petals, anthers or pistils of male sterile line. Further, inhibition of the CaMF2 by virus-induced gene silencing (VIGS) method resulted in the low pollen germination ability and shriveled pollen grains. All these evidence showed that CaMF2 had a vital role in pollen development of C. annuum.
authors: College of Horticulture, South China Agricultural University, Guangzhou, China.
authors: Changming
authors: C
authors: Chen
authors: Guoju
authors: G
authors: Chen
authors: Xuefeng
authors: X
authors: Hao
authors: Bihao
authors: B
authors: Cao
authors: Qinghua
authors: Q
authors: Chen
authors: Shaoqun
authors: S
authors: Liu
authors: Jianjun
authors: J
authors: Lei
mesh_terms: D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D052179:Antigens, Plant / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002212:Capsicum / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D051479:Plant Infertility / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; D012333:RNA, Messenger; C511920:lipid transfer proteins, plant