title: Amplified multiplexed analysis of DNA by the exonuclease III-catalyzed regeneration of the target DNA in the presence of functionalized semiconductor quantum dots.
abstract: Quantum dots (QDs) functionalized with a black-hole quencher are used as optical tracer for the detection of DNA using exonuclease as a biocatalyst. The binding of the target DNA or of a target/open hairpin complex to the functionalized QDs leads to the exonuclease-stimulated recycling of the target DNA or the target/hairpin complex. This results in the triggering of the luminescence of the QDs that provides a readout signal for the amplified sensing process. By using different-sized QDs, the multiplexed detection of DNAs is demonstrated.
authors: Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors: Ronit
authors: R
authors: Freeman
authors: Xiaoqing
authors: X
authors: Liu
authors: Itamar
authors: I
authors: Willner
mesh_terms: D001483:Base Sequence; D055162:Biocatalysis; D004247:DNA / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D045663:Quantum Dots; D012666:Semiconductors
chemical_list: D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III