title: A new technique for visualizing proanthocyanidins by light microscopy.
abstract: We describe a new technique for visualizing proanthocyanidin-containing elements in plant tissues. Our innovation is the fixation of condensed tannins with an exogenous protein prior to alcohol dehydration. In this way, tannins do not undergo partial solubilization during the dehydration sequence and appear as sharply contoured globules of various diameters.
authors: UMR OEnological Science, National Institute for Agronomic Research-University of Montpellier I (INRA-UMI), Montpellier, France. brilloue@supagro.inra.fr
authors: J-M
authors: JM
authors: Brillouet
authors: J
authors: J
authors: Escoute
mesh_terms: D002110:Caffeine; D004396:Coloring Agents; D003681:Dehydration; D006454:Hemoglobins; D006651:Histocytochemistry / Q000379:methods*; D008853:Microscopy; D010944:Plants / Q000378:metabolism*; D044945:Proanthocyanidins / Q000378:metabolism*; D012995:Solubility; D013194:Staining and Labeling / Q000379:methods; D014018:Tissue Distribution
chemical_list: D004396:Coloring Agents; D006454:Hemoglobins; D044945:Proanthocyanidins; D002110:Caffeine