title: MRI of transplanted surface-labeled pancreatic islets with heparinized superparamagnetic iron oxide nanoparticles.
abstract: Transplantation of insulin-secreting pancreatic islets can provide real-time regulation of blood glucose in patients with type 1 diabetes mellitus. Currently, noninvasive and repetitive monitoring of islet engraftment and function is an emerging and promising modality for successful islet transplantation. Here we report a new technique for highly sensitive in vivo magnetic resonance (MR) imaging of transplanted pancreatic islets. To this end, heparinized superparamagnetic iron oxide (heparin-SPIO) nanoparticle was newly synthesized for chemical conjugation onto islet surface. Compared to typical cellular labeling of Feridex(®) via random endocytosis, chemical conjugation of heparin-SPIO was stable and improved the hypointensity of transplanted islets due to surface modification of every islet. These heparin-SPIO-conjugated islets showed normal viability and insulin secretion, and were quantified by spin echo T(2)-weighted MR imaging with linear correlation depending on transplanted islet mass in vitro and in vivo for 30 days. Also, from the immunohistochemistry, we confirmed the existence of heparin-SPIO and insulin biosynthesis in transplanted islets. However, Feridex-uptake islets showed late glucose responsiveness according to changing glucose concentration although they could normally control the blood glucose levels in diabetic mouse. Thus, we anticipate that this surface labeling with heparin-SPIO can be directly applicable for MR imaging of transplanted islets.
authors: Department of Bioengineering, College of Engineering, and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, Republic of Korea.
authors: Min Jin
authors: MJ
authors: Jung
authors: Seung Soo
authors: SS
authors: Lee
authors: Yong Hwa
authors: YH
authors: Hwang
authors: Hae Song
authors: HS
authors: Jung
authors: Jin Wook
authors: JW
authors: Hwang
authors: Min Jun
authors: MJ
authors: Kim
authors: Sangwoo
authors: S
authors: Yoon
authors: Dong Yun
authors: DY
authors: Lee
mesh_terms: D000818:Animals; D003911:Dextrans / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000473:pathology / Q000628:therapy; D006493:Heparin / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D016381:Islets of Langerhans Transplantation; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013113:Spin Labels; D013194:Staining and Labeling; D013499:Surface Properties / Q000187:drug effects
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D013113:Spin Labels; D006493:Heparin; C065507:ferumoxides