title: Fluorescence microscopy of nanochannel-confined DNA.
abstract: Stretching of DNA in nanoscale confinement allows for direct visualization of the genetic contents of the DNA on the single DNA molecule level. DNA stretched in nanoscale confinement also allows for studies of DNA-protein interactions and DNA polymer physics in confined environments. This chapter describes the basic steps to fabricate the nanostructures, to perform the experiments, and to analyze the data.
authors: Department of Physics, University of Gothenburg, Gothenburg, Sweden.
authors: Fredrik
authors: F
authors: Persson
authors: Fredrik
authors: F
authors: Westerlund
authors: Jonas O
authors: JO
authors: Tegenfeldt
mesh_terms: D004247:DNA / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D004247:DNA