title: Cellular human CLE/C14orf166 protein interacts with influenza virus polymerase and is required for viral replication.
abstract: The influenza A virus polymerase associates with a number of cellular transcription-related factors, including RNA polymerase II. We previously described the interaction of influenza virus polymerase subunit PA with human CLE/C14orf166 protein (hCLE), a positive modulator of this cellular RNA polymerase. Here, we show that hCLE also interacts with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins. Silencing of hCLE causes reduction of viral polymerase activity, viral RNA transcription and replication, virus titer, and viral particle production. Altogether, these findings indicate that the cellular transcription factor hCLE is an important protein for influenza virus replication.
authors: Centro Nacional de Biotecnología, C.S.I.C., Darwin 3, Cantoblanco, 28049 Madrid, Spain.
authors: Ariel
authors: A
authors: Rodriguez
authors: Alicia
authors: A
authors: Pérez-González
authors: Amelia
authors: A
authors: Nieto
mesh_terms: D002460:Cell Line; D054884:Host-Pathogen Interactions; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000502:physiology*; D008969:Molecular Sequence Data; D011485:Protein Binding; D025941:Protein Interaction Mapping; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014779:Virus Replication
chemical_list: C435504:RTRAF protein, human; D015534:Trans-Activators; D012324:RNA-Dependent RNA Polymerase