title: [Study of resistance to chemotherapy mediated by ABC transporters in biopsies of glioblastoma multiforme].
abstract: BACKGROUND
  Mortality rate is dramatically high in high grade brain tumors. The presence of multiple drug resistance transporters in glioblastoma multiforme, has contributed largely to the poor efficacy of targeted therapy against cancer in the central nervous system.
  
  
  OBJECTIVE
  To analyze the percentage of survival and mortality of patients with glioblastoma multiforme in a cohort of patients in Chile and to co-rrelate the chemo-resistance of these cells with the expression level of multiple drug resistance transporters.
  
  
  METHODS
  Eighteen biopsies of glioblastoma multiforme were obtained from patients at the Institute of Neurosurgery Dr. Asenjo (INCA). The tumor cells were obtained from primary cultures and the expression and activity of multiple drug resistance transporters was assessed by RT-PCR and immunohistochemistry. Population-based study was performed using the databases of the Department of Neurosurgery of INCA.
  
  
  RESULTS
  The number of patients with glioblastoma multiforme increased between 2007 and 2009, from 3.5% to 7.9% of total brain tumors. Mortality of these tumors is 90 % at three years. A high expression and activity of the multiple drugs resistance associated protein 1 (Mrp1) transporter was observed in primary cultures of biopsies.
  
  
  CONCLUSIONS
  We propose that Mrp1 activity is responsible for the chemo-resistance of the glioblastoma multiforme and inhibition of this transporter could represent a plausible strategy for the treatment.
authors: Instituto de Bioquímica, Universidad Austral de Chile, Valdivia, Chile. claudiaquezada@uach.cl
authors: Claudia
authors: C
authors: Quezada
authors: Lilia
authors: L
authors: Peigñan
authors: Rodrigo
authors: R
authors: Segura
authors: Francisco
authors: F
authors: Riquelme
authors: Rómulo
authors: R
authors: Melo
authors: David
authors: D
authors: Rojas Z
authors: Freddy
authors: F
authors: Ayach
authors: Rody
authors: R
authors: San Martín
authors: Juan Guillermo
authors: JG
authors: Cárcamo
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000401:mortality; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D019008:Drug Resistance, Neoplasm; D005260:Female; D005500:Follow-Up Studies; D005909:Glioblastoma / Q000188:drug therapy* / Q000378:metabolism / Q000401:mortality; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D018528:ATP-Binding Cassette Transporters; D009363:Neoplasm Proteins