title: High-throughput mass finger printing and Lewis blood group assignment of human milk oligosaccharides.
abstract: The structural diversity of human milk oligosaccharides (HMOs) strongly depends on the Lewis (Le) blood group status of the donor which allows a classification of these glycans into three different groups. Starting from 50 μL of human milk, a new high-throughput, standardized, and widely automated mass spectrometric approach has been established which can be used for correlation of HMO structures with the respective Lewis blood groups on the basis of mass profiles of the entire mixture of glycans together with selected fragment ion spectra. For this purpose, the relative abundance of diagnostically relevant compositional species, such as Hex(2)Fuc(2) and Hex(3)HexNAc(1)Fuc(2), as well as the relative intensities of characteristic fragment ions obtained thereof are of key importance. For each Lewis blood group, i.e., Le(a-b+), Le(a+b-), and Le(a-b-), specific mass profile and fragment ion patterns could be thus verified. The described statistically proven classification of the derived glycan patterns may be a valuable tool for analysis and comparison of large sets of milk samples in metabolic studies. Furthermore, the outlined protocol may be used for rapid screening in clinical studies and quality control of milk samples donated to milk banks.
authors: Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University of Giessen, Friedrichstrasse 24, 35392 Giessen, Germany.
authors: Dennis
authors: D
authors: Blank
authors: Sabine
authors: S
authors: Gebhardt
authors: Kai
authors: K
authors: Maass
authors: Günter
authors: G
authors: Lochnit
authors: Viktoria
authors: V
authors: Dotz
authors: Jennifer
authors: J
authors: Blank
authors: Rudolf
authors: R
authors: Geyer
authors: Clemens
authors: C
authors: Kunz
mesh_terms: D002240:Carbohydrate Sequence; D016002:Discriminant Analysis; D005260:Female; D057166:High-Throughput Screening Assays / Q000191:economics / Q000379:methods; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000032:analysis*; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*
chemical_list: D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides