title: State of heat shock factor 1 expression as a putative diagnostic marker             for oral squamous cell carcinoma.
abstract: Heat shock factor 1 (HSF1) is responsible for expres-- sion of a large class             of heat shock proteins that have been implicated in the malignant phenotype of             human cancers. Little is known about the effect of a high level of HSF1 on the             behavior of oral squamous cell carcinoma (OSCC). In this study, we assessed the             value of HSF1 for predicting clinical outcomes in OSCC. Quantitative reverse transcriptase-polymerase             chain reaction and Western blotting showed that the expressions of HSF1 mRNA and             protein in OSCC-derived cell lines (HSC-2, HSC-3, HSC-4, Sa3, Ca9-22, KON and             Ho-1-u-1) were elevated compared with those in human normal oral keratinocytes             (P<0.05). Similar to in vitro data, HSF1 mRNA expression in primary OSCCs (n=50)             was significantly greater than in normal counterparts (P<0.05). Since HSF1             was observed in the nucleus and cytoplasm by immu-- nohistochemistry, we investigated             the correlation between the HSF1 expression status at each subcellular location             and the clinical behavior of OSCCs. Among the clinical classifications, higher             nuclear HSF1 expression was closely related to tumor size and histopathologic             types (P<0.05). These results showed for the first time that nuclear HSF1 expression             may contribute to cancer progression and that HSF1 might be a potential diagnostic             biomarker and a therapeutic target for OSCCs.
authors: Department of Clinical Molecular Biology, Graduate School of                     Medicine, Chiba University, Chiba 260-8670, Japan.
authors: Junya
authors: J
authors: Ishiwata
authors: Atsushi
authors: A
authors: Kasamatsu
authors: Kentaro
authors: K
authors: Sakuma
authors: Manabu
authors: M
authors: Iyoda
authors: Masanobu
authors: M
authors: Yamatoji
authors: Katsuya
authors: K
authors: Usukura
authors: Shunsaku
authors: S
authors: Ishige
authors: Toshihiro
authors: T
authors: Shimizu
authors: Yukio
authors: Y
authors: Yamano
authors: Katsunori
authors: K
authors: Ogawara
authors: Masashi
authors: M
authors: Shiiba
authors: Hideki
authors: H
authors: Tanzawa
authors: Katsuhiro
authors: K
authors: Uzawa
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D000076249:Heat Shock Transcription Factors; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C000619706:HSF1 protein, human; D000076249:Heat Shock Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors