title: Neural-specific elongation of 3' UTRs during Drosophila development.
abstract: The 3' termini of eukaryotic mRNAs influence transcript stability, translation efficiency, and subcellular localization. Here we report that a subset of developmental regulatory genes, enriched in critical RNA-processing factors, exhibits synchronous lengthening of their 3' UTRs during embryogenesis. The resulting UTRs are up to 20-fold longer than those found on typical Drosophila mRNAs. The large mRNAs emerge shortly after the onset of zygotic transcription, with several of these genes acquiring additional, phased UTR extensions later in embryogenesis. We show that these extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon documented in this study. These findings suggest a previously unknown mode of posttranscriptional regulation that may contribute to the complexity of neurogenesis or neural function.
authors: Division of Genetics, Genomics, and Development, Department of Molecular and Cell Biology, Center for Integrative Genomics, University of California, Berkeley, CA 94720-3200, USA.
authors: Valérie
authors: V
authors: Hilgers
authors: Michael W
authors: MW
authors: Perry
authors: David
authors: D
authors: Hendrix
authors: Alexander
authors: A
authors: Stark
authors: Michael
authors: M
authors: Levine
authors: Benjamin
authors: B
authors: Haley
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology / Q000378:metabolism*; D059372:Nucleotide Motifs / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C498778:Imp protein, Drosophila; D016601:RNA-Binding Proteins; C412783:brat protein, Drosophila; C074333:pum protein, Drosophila