title: Improvement of polygalacturonase production at high temperature by mixed culture of Aspergillus niger and Saccharomyces cerevisiae.
abstract: Catabolic repression in the synthesis of inducible enzymes by glucose, fructose, and intermediates of the glycolytic cycle has been observed in many microorganisms. In order to enhance the polygalacturonase (PG) production of Aspergillus niger GJ-2, Saccharomyces cerevisiae J-1 was inoculated to the medium at 12h of culture, which resulted in a significant improvement of PG production. It was also found that maximum PG activity of 512.7 U/ml was obtained at 37°C in the mixed culture, which was nearly twofold higher than that of the culture without the inoculation of S. cerevisiae J-1.
authors: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, JiangNan University, 1800# Lihu Road, Wuxi 214122, JiangSu Province, PR China.
authors: Jie-Min
authors: JM
authors: Zhou
authors: Xiang-Yang
authors: XY
authors: Ge
authors: Wei-Guo
authors: WG
authors: Zhang
mesh_terms: D001234:Aspergillus niger / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D032084:Citrus sinensis / Q000737:chemistry; D006358:Hot Temperature; D011096:Polygalacturonase / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis
chemical_list: D014866:Waste Products; D011096:Polygalacturonase