title: The role of Kupffer cells in rat liver regeneration revealed by cell-specific microarray analysis.
abstract: Liver regeneration after partial hepatectomy is a process with various types of cells involved. The role of Kupffer cells (KCs) in liver regeneration is still controversial. In this study we isolated KCs from regenerating liver and conducted cell-specific microarray analysis. The results demonstrated that the controversial role of KCs in liver regeneration could be explained with the expression patterns of TGF-α, IL-6, TNF, and possibly IL-18 during liver regeneration. IL-18 may play an important role in negative regulation of liver regeneration. The functional profiles of gene expression in KCs also indicated that KC signaling might play a negative role in cell proliferation: signaling genes were down regulated before cell division. Immune response genes in KCs were also down regulated during liver regeneration, demonstrating similar expression profiles to that of hepatocytes. The expression patterns of key genes in these functional categories were consistent with the temporal functional profiles.
authors: Key Laboratory of Cell Differentiation and Regulation, Henan Normal University, Xinxiang 453007, China. xucs@x263.net
authors: Cun-Shuan
authors: CS
authors: Xu
authors: Yun
authors: Y
authors: Jiang
authors: Lian-Xing
authors: LX
authors: Zhang
authors: Cui-Fang
authors: CF
authors: Chang
authors: Gai-Ping
authors: GP
authors: Wang
authors: Rui-Jia
authors: RJ
authors: Shi
authors: Yan-Jie
authors: YJ
authors: Yang
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006498:Hepatectomy / Q000379:methods; D020382:Interleukin-18 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007728:Kupffer Cells / Q000378:metabolism* / Q000502:physiology*; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000235:genetics / Q000502:physiology*; D046228:Microarray Analysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000235:genetics; D016211:Transforming Growth Factor alpha / Q000378:metabolism; D048069:Tumor Necrosis Factors / Q000378:metabolism
chemical_list: D020382:Interleukin-18; D015850:Interleukin-6; D016211:Transforming Growth Factor alpha; D048069:Tumor Necrosis Factors