title: X-ray structure of a galactose-specific lectin from Spatholobous parviflorous.
abstract: A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a=60.792 Å, b=60.998 Å, c=78.179 Å, α=78.68°, β=88.62°, γ=104.32°. The data were collected at 2.04 Å resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca(2+) and Mn(2+), bound to a loop region of each chain. The SPL monomers are in jelly roll form.
authors: Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, 670661 Kerala, India.
authors: K
authors: K
authors: Geethanandan
authors: Joseph
authors: J
authors: Abhilash
authors: S R
authors: SR
authors: Bharath
authors: C
authors: C
authors: Sadasivan
authors: M
authors: M
authors: Haridas
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry; D008345:Manganese / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; D008345:Manganese; D002118:Calcium; D005690:Galactose