title: [Preparation and identification of OmpW monoclonal antibodies].
abstract: OBJECTIVE
  To prepare and characterize the mouse monoclonal antibodies against Vibrio parahaemolyticus OmpW.
  
  
  METHODS
  The OmpW amino acid sequence from three diseased Vibrio was analyzed by Bioinformatics. Mice were immunized with r-OmpW which was highly expressed and purified in E.coli. Five Vibrio(Va, Vp, Vh, Vv, Van) were chosen as antigen for mAb selection.The characters of the anti-OmpW monoclonal antibodies were studied by Western blot, Flow Cytometry, indirect immunofluorescence.
  
  
  RESULTS
  OmpW was testified a highly conservative membrane protein.Three clones of anti-OmpW mAb was obtained. The Ig subclass of the mAb secreted from fused cell S5C10 was IgG3, which of the titer was 4.6×10(4);. The mAb could specifically recognize Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, Vibrio anguillarum, Vibrio vulnificus, which could not react with Pseudomonas flurosecens, Aeromonas hydrophila, Aeromonas sobria, Aeromonas hydrophila, Escherichia coli.
  
  
  CONCLUSIONS
  The mAb could specially recognize five diseased Vibrio, which is a useful tool for the further study of the diagnosis of Vibrio.
authors: Institute of Pharmaceutical Biotechnology and Bioengineering, Fuzhou University, Fuzhou 350002, China. zhenningma2008@yahoo.cn
authors: Zhen-Ning
authors: ZN
authors: Ma
authors: Lei
authors: L
authors: Zheng
authors: Hai-Mei
authors: HM
authors: Lin
authors: Yun-Quan
authors: YQ
authors: Zheng
authors: Feng-Xiang
authors: FX
authors: Tang
authors: Xian-Ai
authors: XA
authors: Shi
authors: Yang-Hao
authors: YH
authors: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins