title: The expression and localization of Prune2 mRNA in the central nervous system.
abstract: A family of Bcl-2/adenovirus E1B 19kDa-interacting proteins (BNIPs) plays critical roles in several cellular processes such as cellular transformation, apoptosis, neuronal differentiation, and synaptic function, which are mediated by the BNIP2 and Cdc42GAP homology (BCH) domain. Prune homolog 2 (Drosophila) (PRUNE2) and its isoforms -C9orf65, BCH motif-containing molecule at the carboxyl terminal region 1 (BMCC1), and BNIP2 Extra Long (BNIPXL) - have been shown to be a susceptibility gene for Alzheimer's disease, a biomarker for leiomyosarcomas, a proapoptotic protein in neuronal cells, and an antagonist of cellular transformation, respectively. However, precise localization of PRUNE2 in the brain remains unclear. Here, we identified the distribution of Prune2 mRNA in the adult mouse brain. Prune2 mRNA is predominantly expressed in the neurons of the cranial nerve motor nuclei and the motor neurons of the spinal cord. The expression in the dorsal root ganglia (DRG) is consistent with the previously described reports. In addition, we observed the expression in another sensory neuron in the mesencephalic trigeminal nucleus. These results suggest that Prune2 may be functional in these restricted brain regions.
authors: Department of Neurobiology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
authors: Shimo
authors: S
authors: Li
authors: Masanori
authors: M
authors: Itoh
authors: Kazunori
authors: K
authors: Ohta
authors: Masashi
authors: M
authors: Ueda
authors: Akihito
authors: A
authors: Mizuno
authors: Eri
authors: E
authors: Ohta
authors: Yoko
authors: Y
authors: Hida
authors: Miao-Xing
authors: MX
authors: Wang
authors: Kazunori
authors: K
authors: Takeuchi
authors: Toshiyuki
authors: T
authors: Nakagawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics; D002490:Central Nervous System / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003391:Cranial Nerves / Q000201:enzymology / Q000378:metabolism; D016208:Databases, Factual; D005091:Exons / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000201:enzymology / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D009046:Motor Neurons / Q000201:enzymology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011984:Sensory Receptor Cells / Q000201:enzymology; D013116:Spinal Cord / Q000201:enzymology; D014278:Trigeminal Nuclei / Q000201:enzymology
chemical_list: D009363:Neoplasm Proteins; C560677:PRUNE2 protein, mouse; D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase