title: Quantitative analysis of the combined effect of substrate rigidity and topographic guidance on cell morphology.
abstract: Live cells are exquisitely sensitive to both the substratum rigidity and texture. To explore cell responses to both these types of inputs in a precisely controlled fashion, we analyzed the responses of Chinese hamster ovary (CHO) cells to nanotopographically defined substrata of different rigidities, ranging from 1.8 MPa to 1.1 GPa. Parallel arrays of nanogrooves (800-nm width, 800-nm space, and 800-nm depth) on polyurethane (PU)-based material surfaces were fabricated by UV-assisted capillary force lithography (CFL) over an area of 5 mm × 3 mm. We observed dramatic morphological responses of CHO cells, evident in their elongation and polarization along the nanogrooves direction. The cells were progressively more spread and elongated as the substratum rigidity increased, in an integrin β1 dependent manner. However, the degree of orientation was independent of substratum rigidity, suggesting that the cell shape is primarily determined by the topographical cues.
authors: Department of Biomedical Engineering and Institutefor Cell Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. jpark145@ jhmi.edu
authors: JinSeok
authors: J
authors: Park
authors: Hong-Nam
authors: HN
authors: Kim
authors: Deok-Ho
authors: DH
authors: Kim
authors: Andre
authors: A
authors: Levchenko
authors: Kahp-Yang
authors: KY
authors: Suh
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000502:physiology*; D003585:Cell Biology / Q000295:instrumentation*; D048430:Cell Shape / Q000502:physiology*; D060833:Cellular Microenvironment; D006224:Cricetinae; D003412:Cricetulus; D022001:Focal Adhesions / Q000502:physiology; D006244:Hardness; D019012:Integrin beta1 / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D019012:Integrin beta1