title: Hollow fiber liquid-phase microextraction for the determination of nimesulide in human plasma and its application to a pharmacokinetic study.
abstract: A hollow fiber liquid-phase microextraction (HF-LPME) method in combination with HPLC-UV for the determination of nimesulide in human plasma was developed and validated. A small volume of dihexyl ether contained within a polypropylene hollow fiber was used for the extraction of nimesulide from acidified plasma solutions. Factors affecting the extraction efficiency were optimized and discussed. With HPLC-UV as the end analysis technique, the procedure was validated for nimesulide in the concentration range of 50-5000 ng/mL. The intra- and inter-assay precisions were less than 9.1%, and accuracy was within 3.2%. The lower limit of quantification (LLOQ) was 50 ng/mL. Enrichment factor from 144-fold to 156-fold was achieved at three quality control (QC) concentrations. The mean extraction recovery was greater than 41.2%. This method was successfully applied for the evaluation of pharmacokinetics of nimesulide after single oral doses of 100 mg nimesulide to six healthy Chinese volunteers.
authors: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, PR China.
authors: W W
authors: WW
authors: Yang
authors: H F
authors: HF
authors: Zhang
authors: Y R
authors: YR
authors: Jia
authors: T
authors: T
authors: Zhao
authors: Y L
authors: YL
authors: Zhao
authors: L J
authors: LJ
authors: Tong
authors: L X
authors: LX
authors: Sun
mesh_terms: D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007477:Ions; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000097:blood* / Q000493:pharmacokinetics*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007202:Indicators and Reagents; D007477:Ions; D012997:Solvents; D013449:Sulfonamides; C012655:nimesulide