title: Identification of CD105 (endoglin)-positive stem-like cells in rhabdoid meningioma.
abstract: To investigate the tumor-initiating cells (TICs) in rhabdoid meningioma (RM), a population of CD105-positive cells isolated from a fresh RM surgical sample was analyzed for proliferative activity, self-renewal ability, tumorigenic ability, multilineage differentiation potential, as well as chromosomal aberrations. The results showed that isolated CD105-positive cells could be maintained for more than 50 generations in vitro. These cells exhibited increased proliferative activity and single-cell tumor sphere-formation ability compared with CD105-negative cells. In vivo experiments showed that CD105-positive cells possessed much greater potential to reconstitute the original human RM in nude mice as compared with CD105-negative cells. Phenotypically, CD105-positive cells shared some surface markers with mesenchymal progenitor cells (MPCs), but karyotype analysis showed chromosomal abnormalities characteristic of malignant meningioma, thus distinguishing them from supportive stroma-derived MPCs. In addition, in contrast to CD105-negative cells, CD105-positive cells could differentiate into adipocytes and osteocytes in response to specific induction agents. Finally, CD105-positive cells with stem-like features were also isolated from xenograft tumors. In conclusion, a population of CD105-positive TICs with some traits of MPCs was identified in RM and might provide a promising therapeutic target in management of malignant meningioma.
authors: Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
authors: Dezhi
authors: D
authors: Hu
authors: Xiaomei
authors: X
authors: Wang
authors: Ying
authors: Y
authors: Mao
authors: Liangfu
authors: L
authors: Zhou
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D018957:CD59 Antigens / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D000071063:Endoglin; D005434:Flow Cytometry; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D059785:Karyotype; D019394:Ki-67 Antigen / Q000378:metabolism; D008577:Meningeal Neoplasms / Q000473:pathology*; D008579:Meningioma / Q000473:pathology*; D018396:Mucin-1 / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018335:Rhabdoid Tumor / Q000473:pathology*; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D018957:CD59 Antigens; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D019394:Ki-67 Antigen; D018396:Mucin-1; D011956:Receptors, Cell Surface; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; D014746:Vimentin