title: An improved cell isolation method for flow cytometric and functional analyses of cutaneous wound leukocytes.
abstract: Isolation of leukocytes from full-thickness excisional wounds has proven to be a difficult process that results in poor cell yield and holds significant limitations for functional assays. Given the increased interest in the isolation, characterization and functional measurements of wound-derived cell populations, herein we describe a method for preparing wound cell suspensions with an improved yield that enables both phenotypic and functional assessments.
authors: The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA.
authors: Aleah L
authors: AL
authors: Brubaker
authors: David F
authors: DF
authors: Schneider
authors: Jessica L
authors: JL
authors: Palmer
authors: Douglas E
authors: DE
authors: Faunce
authors: Elizabeth J
authors: EJ
authors: Kovacs
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry / Q000379:methods*; D007962:Leukocytes / Q000276:immunology / Q000473:pathology*; D051379:Mice; D010587:Phagocytosis / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000293:injuries / Q000473:pathology*; D013211:Staphylococcus aureus / Q000276:immunology