title: Four potato (Solanum tuberosum) ABCG transporters and their expression in response to abiotic factors and Phytophthora infestans infection.
abstract: Pleiotropic drug resistant (PDR/ABCG) genes are involved in plant response to biotic and abiotic stresses. In this work, we cloned, from Solanum tuberosum, four PDR/ABCG transporter genes named StPDR1, StPDR2, StPDR3 and StPDR4, which were differentially expressed in plant tissues and cell cultures. A number of different chemically unrelated compounds were found to regulate the transcript levels of the four genes in cultured cells. In particular, StPDR2 was highly up-regulated in the presence of Botrytis cinerea cell walls, NaCl, 2,4-dichlorophenol, sclareol and α-solanin and biological compounds. The expression of the genes was also investigated by real time RT-PCR during infection by Phytophthora infestans. StPDR1 and StPDR2 were up-regulated about 13- and 37-fold at 48 h post-infection (hpi), StPDR3 was expressed (4-5-fold) at 24 and 48 hpi and then rapidly decreased, while StPDR4 RNA accumulation was stimulated (about 4-fold) at 12 and 24 hpi, decreased at 48 hpi and increased again at 96 hpi. We discuss the role of StPDR1-4 genes in response to pathogens and abiotic stresses.
authors: Istituto per la Protezione delle Piante CNR, Via Università 133, 80055 Portici (NA), Italy. ruocco@ipp.cnr.it
authors: Michelina
authors: M
authors: Ruocco
authors: Patrizia
authors: P
authors: Ambrosino
authors: Stefania
authors: S
authors: Lanzuise
authors: Sheridan Lois
authors: SL
authors: Woo
authors: Matteo
authors: M
authors: Lorito
authors: Felice
authors: F
authors: Scala
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D002733:Chlorophenols / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D055750:Phytophthora infestans / Q000472:pathogenicity*; D010940:Plant Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D012992:Solanine / Q000494:pharmacology; D011198:Solanum tuberosum / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology*
chemical_list: D018528:ATP-Binding Cassette Transporters; D002733:Chlorophenols; D010940:Plant Proteins; C514919:alpha-solanine; D012992:Solanine; D012965:Sodium Chloride; C004762:2,4-dichlorophenol