title: Interleukin-15 affects differentiation and apoptosis in adipocytes: implications in obesity.
abstract: Interleukin-15 (IL-15) is an anabolic factor for skeletal muscle and several reports have described its important role as a regulator of energy homeostasis. In this study, we analyzed the effects of IL-15 on adipocyte differentiation using the 3T3-L1 preadipose cell line. The data show that IL-15 tends to reduce the rate of adipocyte proliferation, induces apoptosis, and partially stops differentiation. The signaling molecules behind these actions of the cytokine on adipose cells are: p42/p44 MAPK (which seem to be associated with the reduced rate of proliferation induced by the cytokine), STAT5 (which is related to the actions of IL-15 on differentiation), and SAPK/JNK (which are related to the increased apoptosis induced by IL-15). In conclusion, using the 3T3-L1 adipocyte cell line, the results presented here show that IL-15 exerts important effects on differentiation, proliferation and apoptosis. Altogether, the results presented here reinforce the idea that IL-15 is an important mediator that regulates adipose size and, therefore, the role of the cytokine in affecting body weight and obesity deserves additional studies.
authors: Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Cancer Research Group, Universitat de Barcelona, Diagonal 645, 08028, Barcelona, Spain.
authors: Gemma
authors: G
authors: Fuster
authors: Vanessa
authors: V
authors: Almendro
authors: Cibely Cristine
authors: CC
authors: Fontes-Oliveira
authors: Míriam
authors: M
authors: Toledo
authors: Paola
authors: P
authors: Costelli
authors: Sílvia
authors: S
authors: Busquets
authors: Francisco J
authors: FJ
authors: López-Soriano
authors: Josep M
authors: JM
authors: Argilés
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000502:physiology*; D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001391:Azo Compounds; D002454:Cell Differentiation; D049109:Cell Proliferation; D015870:Gene Expression; D019409:Interleukin-15 / Q000494:pharmacology / Q000502:physiology*; D020935:MAP Kinase Signaling System; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009765:Obesity / Q000473:pathology*
chemical_list: D000943:Antigens, Differentiation; D051017:Apoptosis Regulatory Proteins; D001391:Azo Compounds; D019409:Interleukin-15; D020928:Mitogen-Activated Protein Kinases; C011049:oil red O