title: Producing reverse phase protein microarrays from formalin-fixed tissues.
abstract: In most hospitals around the world FFPE (formalin fixed, paraffin embedded) tissues have been used for diagnosis and have subsequently been archived since decades. This has lead to a sizeable pool of this kind of tissues. Till quite recently it was not possible to use this congeries of samples for protein analysis, but now several groups described successful protein extraction from FFPE tissues. In this chapter, we describe a protein extraction protocol established in our laboratory combined with the use of reverse phase protein microarray.
authors: Institut für Pathologie, Technische Universität München, Munich, Germany.
authors: Claudia
authors: C
authors: Wolff
authors: Christina
authors: C
authors: Schott
authors: Katharina
authors: K
authors: Malinowsky
authors: Daniela
authors: D
authors: Berg
authors: Karl-Friedrich
authors: KF
authors: Becker
mesh_terms: D005557:Formaldehyde; D049449:Luminescence; D016612:Paraffin Embedding / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D011506:Proteins; D005557:Formaldehyde