title: FUdR causes a twofold increase in the lifespan of the mitochondrial mutant gas-1.
abstract: The nematode worm Caenorhabditis elegans has been used to identify hundreds of genes that influence longevity and thereby demonstrate the strong influence of genetics on lifespan determination. In order to simplify lifespan studies in worms, many researchers have employed 5-fluoro-2'-deoxyuridine (FUdR) to inhibit the development of progeny. While FUdR has little impact on the lifespan of wild-type worms, we demonstrate that FUdR causes a dramatic, dose-dependent, twofold increase in the lifespan of the mitochondrial mutant gas-1. Thus, the concentration of FUdR employed in a lifespan study can determine whether a particular strain is long-lived or short-lived compared to wild-type.
authors: Department of Biology, McGill University, Montreal, Quebec, Canada H2X 3H5.
authors: Jeremy Michael
authors: JM
authors: Van Raamsdonk
authors: Siegfried
authors: S
authors: Hekimi
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D005467:Floxuridine / Q000008:administration & dosage / Q000494:pharmacology*; D017238:Genes, Helminth; D050259:Genes, Mitochondrial; D008136:Longevity / Q000187:drug effects* / Q000235:genetics*; D009154:Mutation; D009245:NADH Dehydrogenase / Q000235:genetics*
chemical_list: D029742:Caenorhabditis elegans Proteins; D005467:Floxuridine; D009245:NADH Dehydrogenase; C468009:GAS-1 protein, C elegans