title: Sugarcane Hsp101 is a hexameric chaperone that binds nucleotides.
abstract: The Clp/Hsp100 AAA+ chaperone family is involved in recovering aggregated proteins and little is known about other orthologs of the well studied ClpB from Escherichia coli and Hsp104 from Saccharomyces cerevisiae. Plant Hsp101 is a good model for understanding the relationship between the structure and function of Hsp100 proteins and to investigate the role of these chaperones in disaggregation processes. Here, we present the cloning and purification of a sugarcane ortholog, SHsp101, which is expressed in sugarcane cells and is a folded hexamer that is capable of binding nucleotides. Thus SHsp101 has the structural and functional characteristics of the Clp/Hsp100 AAA+ family.
authors: Institute of Chemistry, University of Campinas-UNICAMP, PO Box 6154, 13083-970, Campinas, SP, Brazil.
authors: Thiago C
authors: TC
authors: Cagliari
authors: Viviane C H
authors: VC
authors: da Silva
authors: Júlio C
authors: JC
authors: Borges
authors: Alessandra
authors: A
authors: Prando
authors: Ljubica
authors: L
authors: Tasic
authors: Carlos H I
authors: CH
authors: Ramos
mesh_terms: D000595:Amino Acid Sequence; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids; D058105:Polymerization; D011485:Protein Binding; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014461:Ultracentrifugation
chemical_list: D029968:Escherichia coli Proteins; C115569:HSP101 protein, plant; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009711:Nucleotides; D010940:Plant Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C070972:HsP104 protein, S cerevisiae; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli