title: Simultaneous "one pot" expressed protein ligation and CuI-catalyzed azide/alkyne cycloaddition for protein immobilization.
authors: Institute of Biochemistry, Universität Leipzig, Leipzig, Germany. beck-sickinger@uni-leipzig.de
authors: Max
authors: M
authors: Steinhagen
authors: Kai
authors: K
authors: Holland-Nell
authors: Morten
authors: M
authors: Meldal
authors: Annette G
authors: AG
authors: Beck-Sickinger
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D057930:Click Chemistry; D003300:Copper / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence
chemical_list: D000480:Alkynes; D001386:Azides; D055767:Immobilized Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D003300:Copper