title: In vivo release of bovine serum albumin from an injectable small intestinal submucosa gel.
abstract: We aimed to develop a delivery system capable of maintaining a sustained release of protein drugs at specific sites using potentially biocompatible biomaterials. Here, we used bovine serum albumin (BSA) as a test protein to explore the potential utility of an injectable small intestine submucosa (SIS) as a depot for protein drugs. The prepared SIS powder was dispersed in PBS. The SIS suspension easily entrapped BSA in pharmaceutical formulations at room temperature. When this was suspension subcutaneously injected into rats, it gelled, forming an interconnecting three-dimensional network SIS structure to allow BSA to penetrate through it. The amount of BSA-FITC released from the SIS gel was determined in rat plasma and monitored by real-time in vivo molecular imaging. The data indicated the sustained release of BSA-FITC for 30 days in vivo. In addition, SIS gel provoked little inflammatory response. Collectively, our results show that the SIS gel described here could serve as a minimally invasive therapeutics depot with numerous benefits compared to other injectable biomaterials.
authors: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Republic of Korea.
authors: Kkot Nim
authors: KN
authors: Kang
authors: Da Yeon
authors: DY
authors: Kim
authors: So Mi
authors: SM
authors: Yoon
authors: Jin Seon
authors: JS
authors: Kwon
authors: Hyo Won
authors: HW
authors: Seo
authors: E Sle
authors: ES
authors: Kim
authors: Bong
authors: B
authors: Lee
authors: Jae Ho
authors: JH
authors: Kim
authors: Byoung Hyun
authors: BH
authors: Min
authors: Hai Bang
authors: HB
authors: Lee
authors: Moon Suk
authors: MS
authors: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001682:Biological Availability; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004655:Emulsions; D016650:Fluorescein-5-isothiocyanate / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005782:Gels; D007279:Injections, Subcutaneous; D007583:Jejunum; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D012710:Serum Albumin, Bovine / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D005782:Gels; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate