title: A case of a canine pigmented plaque associated with the presence of a Chi-papillomavirus.
abstract: The seven fully described canine papillomaviruses (CPVs) have been allocated by sequence comparison and other genetic features into three phylogenetic clades. This largely reflects clinical findings, so each sequence of a newly discovered CPV in combination with clinical and pathological details is a valuable piece of evidence. We hypothesize that the genomic sequence of a new CPV can help to predict clinical features and progression, and that this can be tested in subsequent cases. In this case, a 2-year-old female dachshund-mix presented with papillomatosis clinically and histologically characterized as pigmented viral plaques. PCRs using primers evaluated for CPVs successfully amplified papillomavirus (PV) DNA. Sequencing of the products revealed an unknown PV putatively belonging to the PV genus Chi. Rolling circle amplification was used to amplify the entire viral genome. Sequencing revealed a novel PV, designated as CPV8, which was most closely related (63% homology) to the recently discovered CPV4. CPV4 is associated with benign pigmented plaques in pugs. Phylogenetic analysis based on the nucleotide sequences of four viral genes showed that the novel virus was closest to CPV3, CPV4 and CPV5. The presence of viral DNA was confirmed in the lesions by in situ hybridization using specific probes. CPV8 may consequently be regarded as the fourth member of the Chi-papillomavirus genus. All viruses belonging to this genus induce pigmented plaques in dogs. These findings support the hypothesis that genomic sequences can be useful in predicting the clinical features of CPV infection.
authors: Dermatology Department, Clinic for Small Animal Internal Medicine,              Institute of Virology and              Institute for Food Safety and Hygiene, Vetsuisse Faculty, Zürich, Switzerland.
authors: Christian E
authors: CE
authors: Lange
authors: Kurt
authors: K
authors: Tobler
authors: Angelika
authors: A
authors: Lehner
authors: Elisabeth
authors: E
authors: Vetsch
authors: Claude
authors: C
authors: Favrot
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005260:Female; D009508:Nevus, Pigmented / Q000662:veterinary* / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral