title: Functional analysis of abscisic acid 8'-hydroxylase.
abstract: Abscisic acid (ABA) plays an important role in the control of seed dormancy and germination. Identification of hormone metabolism genes from a particular plant species of interest is an essential step in hormone research. The function of these gene products is validated by biochemical analysis using heterologous expression systems, such as E. coli and yeast. ABA 8'-hydroxylase is a subfamily of P450 monooxygenases and is encoded by CYP707A genes. CYP707A catalyzes the committed step in the major ABA catabolic pathway. In this chapter, we describe the methods for RNA extraction from seeds, cloning the CYP707A cDNAs, protein expression in yeast, and biochemical analysis of their gene products.
authors: Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.
authors: Akira
authors: A
authors: Endo
authors: Mitsuhiro
authors: M
authors: Kimura
authors: Naoto
authors: N
authors: Kawakami
authors: Eiji
authors: E
authors: Nambara
mesh_terms: D000040:Abscisic Acid / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination / Q000235:genetics / Q000502:physiology*; D053858:Metabolic Networks and Pathways; D057445:Plant Dormancy / Q000235:genetics / Q000502:physiology*; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D012639:Seeds / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; C107203:abscisic acid 8'-hydroxylase