title: Flavonoids and oxidative stress in Drosophila melanogaster.
abstract: Flavonoids are a family of antioxidants that are widely represented in fruits, vegetables, dry legumes, and chocolate, as well as in popular beverages, such as red wine, coffee, and tea. The flavonoids chlorogenic acid, kaempferol, quercetin and quercetin 3β-d-glycoside were investigated for genotoxicity using the wing somatic mutation and recombination test (SMART). This test makes use of two recessive wing cell markers: multiple wing hairs (mwh) and flare (flr(3)), which are mutations located on the left arm of chromosome 3 of Drosophila melanogaster and are indicative of both mitotic recombination and various types of mutational events. In order to test the antioxidant capacities of the flavonoids, experiments were conducted with various combinations of oxidants and polyphenols. Oxidative stress was induced using hydrogen peroxide, the Fenton reaction and paraquat. Third-instar transheterozygous larvae were chronically treated for all experiments. The data obtained in this study showed that, at the concentrations tested, the flavonoids did not induce somatic mutations or recombination in D. melanogaster with the exception of quercetin, which proved to be genotoxic at only one concentration. The oxidants hydrogen peroxide and the Fenton reaction did not induce mutations in the wing somatic assay of D. melanogaster, while paraquat and combinations of flavonoids produced significant numbers of small single spots. Quercetin 3β-d-glycoside mixed with paraquat was shown to be desmutagenic. Combinations of the oxidants with the other flavonoids did not show any antioxidant activity.
authors: Departamento de Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, Copilco Universidad, México, Mexico.
authors: América Nitxin Castañeda
authors: AN
authors: Sotibrán
authors: María Guadalupe
authors: MG
authors: Ordaz-Téllez
authors: Rosario
authors: R
authors: Rodríguez-Arnaiz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000235:genetics; D005419:Flavonoids / Q000494:pharmacology* / Q000633:toxicity*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress; D059808:Polyphenols / Q000633:toxicity
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D009153:Mutagens; D059808:Polyphenols; D006861:Hydrogen Peroxide