title: Reaction conditions for maximal cyclodextrin production by cyclodextrin glucanotransferase from Bacillus megaterium.
abstract: The effect of the reaction conditions (substrate concentration, enzyme dosage, and pH) on cyclodextrin production by cyclodextrin glucanotransferase from Bacillus megaterium was investigated by applying mathematical modeling methods. Adequate models were developed and they were used for determination of the optimal conditions for maximal formation of beta-cyclodextrins at minimal concentrations of a- and gamma-cydclodextrins. The main factor affecting the ratio of the products was pH of the reaction mixture. At pH 9 the enzyme formed mainly beta- and y-cyclodextrins and the ratio a:beta:gamma was 2.6:83.5:13.9; at pH 5 the ratio changed to 8.6:84.6:6.8. Mathematical models were used for determination of the conditions for maximal conversion of the substrate into cyclodextrins. 45.88% conversion of starch was achieved at 5% substrate concentration, 3.5 U/g enzyme dosage, and pH 7.4.
authors: Department of Biochemistry and Molecular Biology, University of Food Technologies, Plovdiv, Bulgaria. zhekova_b@yahoo.com
authors: Boriana Y
authors: BY
authors: Zhekoval
authors: Veselin S
authors: VS
authors: Stanchev
mesh_terms: D000704:Analysis of Variance; D001410:Bacillus megaterium / Q000201:enzymology / Q000378:metabolism*; D003505:Cyclodextrins / Q000096:biosynthesis* / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D012044:Regression Analysis; D013213:Starch / Q000378:metabolism
chemical_list: D003505:Cyclodextrins; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase