title: Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease.
abstract: BACKGROUND
  In this study, we developed a rapid, one step colloid gold strip (CGS) capable of specifically detecting type Asia1 foot-and-mouth disease virus (FMDV). We have produced two monoclonal antibodies (mAb) to type Asia1 FMD (named 1B8 and 5E2). On the test strip, the purified 1B8 labelled with the colloidal gold was used as the detector, and the purified 5E2 and goat anti-mouse antibodies were wrapped onto nitrocellulose (NC) membranes as the test and the control line, respectively. The rapid colloidal gold stereotype diagnostic strip was housed in a plastic case.
  
  
  RESULTS
  In specificity and sensitivity assay, there was no cross-reaction of the antigen with the other type of FMD and SVDV. The detection sensitivity was found to be as high as 10(-5) dilution of Asia1/JSL/05 (1 × 10(7.2)TCID(50)/50 μL). There was excellent agreement between the results obtained by CGS and reverse indirect hemagglutination assay (RIHA), and the agreement can reach to 98.75%.
  
  
  CONCLUSIONS
  We developed colloidal gold strips that have good qualities and does not require specialized equipment or technicians. This method provided a feasible, convenient, rapid, and effective for detecting type Asia1 FMDV in the fields.
authors: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
authors: Tong
authors: T
authors: Lin
authors: Jun-jun
authors: JJ
authors: Shao
authors: Jun-zheng
authors: JZ
authors: Du
authors: Guo-zheng
authors: GZ
authors: Cong
authors: Shan-dian
authors: SD
authors: Gao
authors: Huiyun
authors: H
authors: Chang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000914:Antibodies, Viral / Q000276:immunology* / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D003101:Collodion / Q000737:chemistry / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017979:Gold Colloid / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D006386:Hemagglutination Tests; D007118:Immunoassay; D051379:Mice; D008807:Mice, Inbred BALB C; D011934:Reagent Strips; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D017979:Gold Colloid; D011934:Reagent Strips; D003101:Collodion