title: Antibody microarray analysis of the serum proteome in primary breast cancer patients.
abstract: Noninvasive biomarkers are urgently needed for detecting breast cancer as early as possible since the risk of recurrence, morbidity, and mortality is closely related to disease stage at the time of primary surgery. There are currently no such biomarkers in clinical use as a diagnostic tool. Proteomic analysis of protein expression patterns in body fluids has potential for use in identifying biomarkers of breast cancer. The aim of this study was to compare protein expression levels in the sera of primary breast cancer patients and healthy controls. An antibody microarray tool with 23 antibodies immobilized on nitrocellulose slides was used to determine the levels of acute phase proteins, interleukins, and complement factors in the sera of 101 study participants (49 women with primary breast cancer and 52 healthy age-matched controls). Statistical analysis of reaction intensities identified 6 proteins that showed significantly (p < 0.05) different levels in breast cancer patients vs. healthy subjects. The neural network distinguished cancer patients from controls with a sensitivity of 69% and a specificity of 76%. Thus, antibody microarray analysis could be used as a tool for the development of improved diagnostics and biomarker discovery for breast cancer patients. Further validation of the results and de novo screening of new biomarkers could facilitate the early diagnosis of breast cancer.
authors: Department of Obstetrics and Gynecology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. daniel.boehm@unimedizin-mainz.de
authors: Daniel
authors: D
authors: Böhm
authors: Ksenia
authors: K
authors: Keller
authors: Nils
authors: N
authors: Boehm
authors: Antje
authors: A
authors: Lebrecht
authors: Marcus
authors: M
authors: Schmidt
authors: Heinz
authors: H
authors: Kölbl
authors: Franz-Hermann
authors: FH
authors: Grus
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008875:Middle Aged; D060787:Neoplasm Grading; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome
chemical_list: D014408:Biomarkers, Tumor; D020543:Proteome