title: Mouse lung CD103+ and CD11bhigh dendritic cells preferentially induce distinct CD4+ T-cell responses.
abstract: Mouse lung dendritic cells (LDCs) have been recently shown to contain two major subpopulations: CD103(+) CD11b(low or negative) (CD103(+) LDCs) and CD103(-) CD11b(high) LDCs (CD11b(high) LDCs). Although several studies have demonstrated functional differences between them, it is unclear whether the subpopulations induce distinct T helper (Th) cell responses. The present study was conducted to examine whether CD103(+) and CD11b(high) LDCs preferentially generate different Th responses. Naive DO11.10 CD4(+) T cells were primed with CD103(+) or CD11b(high) LDCs obtained from normal BALB/c mice. The primed CD4(+) T cells were restimulated, and their cytokine secretions were assessed. The expression of intracellular cytokines and the mRNA levels of chemokine receptors were also measured. We found that the CD4(+) T cells primed with CD103(+) LDCs secreted significantly larger amounts of IFN-γ and IL-17A, whereas those primed with CD11b(high) LDCs released significantly higher levels of IL-4, IL-6, and IL-10. Intracellular cytokine assay showed that CD103(+) LDCs induced greater frequencies of CD4(+) T cells producing IFN-γ and IL-17A, whereas CD11b(high) LDCs were more efficient at inducing CD4(+) T cells producing IL-4 and IL-10. The mRNA levels of CXCR3 and CCR5, which are expressed preferentially in Th1 cells, were significantly higher in CD4(+) T cells primed with CD103(+) LDCs. The mRNA levels of CXCR4 and CCR4, which are expressed primarily in Th2 cells, were significantly greater in those primed with CD11b(high) LDCs. These data suggest that mouse CD103(+) LDCs predominantly elicit Th1 and Th17 responses, whereas CD11b(high) LDCs primarily provoke a Th2 response under the steady state.
authors: Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan. suda@hama-med.ac.jp
authors: Kazuki
authors: K
authors: Furuhashi
authors: Takafumi
authors: T
authors: Suda
authors: Hirotsugu
authors: H
authors: Hasegawa
authors: Yuzo
authors: Y
authors: Suzuki
authors: Dai
authors: D
authors: Hashimoto
authors: Noriyuki
authors: N
authors: Enomoto
authors: Tomoyuki
authors: T
authors: Fujisawa
authors: Yutaro
authors: Y
authors: Nakamura
authors: Naoki
authors: N
authors: Inui
authors: Kiyoshi
authors: K
authors: Shibata
authors: Hirotoshi
authors: H
authors: Nakamura
authors: Kingo
authors: K
authors: Chida
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D018845:CD11 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D039001:Integrin alpha Chains / Q000276:immunology*; D008168:Lung / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015703:Antigens, CD; D018845:CD11 Antigens; D039001:Integrin alpha Chains; C091367:alpha E integrins