title: Respiration characteristics of mitochondria in parental and giant transformed cells of the murine Nemeth-Kellner lymphoma.
abstract: Respiration characteristics of mitochondria of the parental and giant cells of murine NK/Ly (Nemeth-Kellner lymphoma) were studied. The giant cell-enriched ascites were obtained by serial intraperitoneal injections of vinblastine in tumour-bearing mice. Ascites containing >70% giant cells were used. Their diameter of was over 17 μm (~2800 μm(3)), while the diameter of the parental cells was 12.7 μm (1100 μm(3)). The respiration rate of mitochondria in situ was measured by oxygen consumption in intact and digitonin-permeabilized NK/Ly cells. Endogenous respiration of intact giant NK/Ly cells was three times higher compared to the parental ones, roughly in agreement with the volume change. The giant NK/Ly cells were far more resistant to permeabilization with digitonin than the parental cells, as shown by Trypan Blue and LDH (lactate dehydrogenase) release tests. After digitonin permeabilization, oxygen consumption was reduced to a minimal level (0.06 ng atom O/(s × 106 cells) in both types of cells. Addition of α-ketoglutarate or succinate to the incubation medium increased oxygen consumption in the parental cells by 46 and 164% respectively. In the giant NK/Ly cells, the corresponding increases were 164 and 276%. Addition of ADP to α-ketoglutarate- or succinate-supplemented medium further stimulated oxygen consumption of the permeabilized NK/Ly cells; however, the effect of ADP was more pronounced in the giant cells. In addition, indices of respiratory control were significantly higher in the giant cells. Oligomycin suppressed considerably the respiration of the intact giant cells but had a much weaker effect on parental cells. Thus, giant NK/Ly cells possess much higher respiration rates and show tighter coupling between the respiration and oxidative phosphorylation compared with parental cells.
authors: Ivan Franko Lviv National University, Lviv, Ukraine.
authors: Rostyslav O
authors: RO
authors: Horbay
authors: Bohdan O
authors: BO
authors: Manko
authors: Volodymyr V
authors: VV
authors: Manko
authors: Maxim D
authors: MD
authors: Lootsik
authors: Rostyslav S
authors: RS
authors: Stoika
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D048429:Cell Size; D004072:Digitonin / Q000494:pharmacology; D015726:Giant Cells / Q000187:drug effects / Q000378:metabolism*; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008223:Lymphoma / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption / Q000187:drug effects; D012119:Respiration / Q000187:drug effects*; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D007656:Ketoglutaric Acids; D009840:Oligomycins; D014747:Vinblastine; D000244:Adenosine Diphosphate; D007770:L-Lactate Dehydrogenase; D004072:Digitonin