title: Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years.
abstract: Human respiratory syncytial virus (HRSV) strains were isolated from nasopharyngeal aspirates collected from 965 children between 2004 and 2005, yielding 424 positive samples. We sequenced the small hydrophobic protein (SH) gene of 117 strains and compared them with other viruses identified worldwide. Phylogenetic analysis showed a low genetic variability among the isolates but allowed us to classify the viruses into different genotypes for both groups, HRSVA and HRSVB. It is also shown that the novel BA-like genotype was well segregated from the others, indicating that the mutations are not limited to the G gene.
authors: Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes 1374, 05508-900 São Paulo, SP, Brazil.
authors: Hildenêr Nogueira
authors: HN
authors: Lima
authors: Viviane Fongaro
authors: VF
authors: Botosso
authors: Danielle Bruna Leal
authors: DB
authors: Oliveira
authors: Angélica Cristine de Almeida
authors: AC
authors: Campos
authors: Andrea Lima
authors: AL
authors: Leal
authors: Tereza Souza
authors: TS
authors: Silva
authors: Patrícia Alves Ramos
authors: PA
authors: Bosso
authors: Claudia Trigo Pedroso
authors: CT
authors: Moraes
authors: Claudionor Gomes da Silva
authors: CG
authors: Filho
authors: Sandra Elisabete
authors: SE
authors: Vieira
authors: Alfredo Elias
authors: AE
authors: Gilio
authors: Klaus Eberhard
authors: KE
authors: Stewien
authors: Edison Luiz
authors: EL
authors: Durigon
mesh_terms: D002675:Child, Preschool; D016000:Cluster Analysis; D005838:Genotype; D006801:Humans; D007223:Infant; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009305:Nasopharynx / Q000821:virology; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology* / Q000821:virology*; D018113:Respiratory Syncytial Virus, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012367:RNA, Viral; D015682:Retroviridae Proteins, Oncogenic; C056150:small hydrophobic protein, virus