title: Genotyping analysis of Helicobacter pylori using multiple-locus variable-number tandem-repeats analysis in five regions of China and Japan.
abstract: BACKGROUND
  H. pylori (Helicobacter pylori) is the major causative agent of chronic active gastritis. The population of H. pylori shows a high genomic variability among isolates. And the polymorphism of repeat-units of genomics had participated the important process of evolution. Its long term colonization of the stomach caused different clinical outcomes, which may relate to the high degree of genetic variation of H. pylori. A variety of molecular typing tools have been developed to access genetic relatedness in H. pylori isolates. However, there is still no standard genotyping system of this bacterium. The MLVA (Multi-locus of variable number of tandem repeat analysis) method is useful for performing phylogenetic analysis and is widely used in bacteria genotyping; however, there's little application in H. pylori analysis. This article is the first application of the MLVA method to investigate H. pylori from different districts and ethnic groups of China.
  
  
  RESULTS
  MLVA of 12 VNTR loci with high discrimination power based on 30 candidates were performed on a collection of 202 strains of H. pylori which originated from five regions of China and Japan. Phylogenetic tree was constructed using MLVA profiles. 12 VNTR loci presented with high various polymorphisms, and the results demonstrated very close relationships between genotypes and ethnic groups.
  
  
  CONCLUSIONS
  This study used MLVA methodology providing a new perspective on the ethnic groups and distribution characteristics of H. pylori.
authors: Department of Clinical Microbiology and Immunology, College of Medical Laboratory, Third Military Medical University, Chongqing, 400038, China.
authors: Chunliang
authors: C
authors: Guo
authors: Yaling
authors: Y
authors: Liao
authors: Yan
authors: Y
authors: Li
authors: Jun
authors: J
authors: Duan
authors: Ying
authors: Y
authors: Guo
authors: Yuqian
authors: Y
authors: Wu
authors: Yujun
authors: Y
authors: Cui
authors: Hongwu
authors: H
authors: Sun
authors: Jinyong
authors: J
authors: Zhang
authors: Bing
authors: B
authors: Chen
authors: Quanming
authors: Q
authors: Zou
authors: Gang
authors: G
authors: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques; D002648:Child; D002681:China; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats; D010802:Phylogeny; D055815:Young Adult