title: Optimization of fumaric acid production by Rhizopus delemar based on the morphology formation.
abstract: The effects of temperature, agitation rate and medium composition, including concentrations of glucose, soybean peptone, and inorganic ions, on pellet formation and pellet diameter of Rhizopus delemar (Rhizopus oryzae) NRRL1526 during pre-culture were studied. Inorganic ions and soybean peptone had negative and positive effects on pellet formation, respectively. The initial glucose and soybean peptone concentrations directly affected pellet diameter. Within a certain range, pellet diameter decreased with increased initial substrate concentrations; however, above this range there was an opposite trend. Thus, optimal concentrations of substrate during pre-culture were beneficial for producing small pellets of R. delemar. Furthermore, dry cell mass and yield of fumaric acid tended to increase with decreased pellet diameter. Based on the pellet morphology optimization, the final fumaric acid concentration was improved by 46.13% when fermented in a flask and 31.82% in stirred bioreactor tank fermentation.
authors: State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors: Zhengxiong
authors: Z
authors: Zhou
authors: Guocheng
authors: G
authors: Du
authors: Zhaozhe
authors: Z
authors: Hua
authors: Jingwen
authors: J
authors: Zhou
authors: Jian
authors: J
authors: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005650:Fumarates / Q000378:metabolism*; D012233:Rhizopus / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D005650:Fumarates; C032005:fumaric acid