title: Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials.
abstract: The biocompatibility and antibacterial properties of N,N-hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro. Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.
authors: Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, United States. irmgard_behlau@meei.harvard.edu
authors: Irmgard
authors: I
authors: Behlau
authors: Koushik
authors: K
authors: Mukherjee
authors: Amit
authors: A
authors: Todani
authors: Ann S
authors: AS
authors: Tisdale
authors: Fabiano
authors: F
authors: Cade
authors: Liqiang
authors: L
authors: Wang
authors: Elizabeth M
authors: EM
authors: Leonard
authors: Fouad R
authors: FR
authors: Zakka
authors: Michael S
authors: MS
authors: Gilmore
authors: Frederick A
authors: FA
authors: Jakobiec
authors: Claes H
authors: CH
authors: Dohlman
authors: Alexander M
authors: AM
authors: Klibanov
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D003315:Cornea / Q000166:cytology / Q000187:drug effects; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology*; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology; D019736:Prostheses and Implants; D011817:Rabbits; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011094:Polyethyleneimine; D019904:Polymethyl Methacrylate; D014025:Titanium