title: TsAg5, a Taenia solium cysticercus protein with a marginal trypsin-like activity in the diagnosis of human neurocysticercosis.
abstract: Neurocysticercosis is an endemic parasitic disease caused by Taenia solium larva. Although the mechanism of infection is not completely understood, it is likely driven by proteolytic activity that degrades the intestinal wall to facilitate oncosphere penetration and further infection. We analyzed the publicly available T. solium EST/DNA library and identified two contigs comprising a full-length cDNA fragment very similar to Echinococcus granulosus Ag5 protein. The T. solium cDNA sequence included a proteolytic trypsin-like-domain in the C-terminal region, and a thrombospondin type-1 adherence-domain in the N-terminal region. Both the trypsin-like and adherence domains were expressed independently as recombinant proteins in bacterial systems. TsAg5 showed marginal trypsin-like activity and high sequence similarity to Ag5. The purified antigens were tested in a Western immunoblot assay to diagnose human neurocysticercosis. The sensitivity of the trypsin-like-domain was 96.36% in patients infected with extraparenchymal cysts, 75.44% in patients infected with multiple cysts, and 39.62% in patients with a single cyst. Specificity was 76.70%. The thrombospondin type-1 adherence-domain was not specific for neurocysticercosis.
authors: Laboratorio de Bioinformática y Biología Molecular, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru.
authors: Analiz
authors: A
authors: Rueda
authors: Cecilia
authors: C
authors: Sifuentes
authors: Robert H
authors: RH
authors: Gilman
authors: Andrés H
authors: AH
authors: Gutiérrez
authors: Ruby
authors: R
authors: Piña
authors: Nancy
authors: N
authors: Chile
authors: Sebastián
authors: S
authors: Carrasco
authors: Sandra
authors: S
authors: Larson
authors: Holger
authors: H
authors: Mayta
authors: Manuela
authors: M
authors: Verástegui
authors: Silvia
authors: S
authors: Rodriguez
authors: Marcel
authors: M
authors: Gutiérrez-Correa
authors: Héctor H
authors: HH
authors: García
authors: Patricia
authors: P
authors: Sheen
authors: Mirko
authors: M
authors: Zimic
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003552:Cysticercus / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000469:parasitology; D017434:Protein Structure, Tertiary; D013552:Swine; D041201:Taenia solium / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013622:Taeniasis / Q000175:diagnosis* / Q000469:parasitology; D014357:Trypsin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D014357:Trypsin