title: Fusarium proliferatum, an endophytic fungus from Dysoxylum binectariferum Hook.f, produces rohitukine, a chromane alkaloid possessing anti-cancer activity.
abstract: Rohitukine is a chromane alkaloid possessing anti-inflammatory, anti-cancer and immuno-modulatory properties. The compound was first reported from Amoora rohituka (Meliaceae) and later from Dysoxylum binectariferum (Meliaceae) and Schumanniophyton problematicum (Rubiaceae). Flavopiridol, a semi-synthetic derivative of rohitukine is a potent CDK inhibitor and is currently in Phase III clinical trials. In this study, the isolation of an endophytic fungus, Fusarium proliferatum (MTCC 9690) from the inner bark tissue of Dysoxylum binectariferum Hook.f (Meliaceae) is reported. The endophytic fungus produces rohitukine when cultured in shake flasks containing potato dextrose broth. The yield of rohitukine was 186 μg/100 g dry mycelial weight, substantially lower than that produced by the host tissue. The compound from the fungus was authenticated by comparing the LC-HRMS and LC-HRMS/MS spectra with those of the reference standard and that produced by the host plant. Methanolic extract of the fungus was cytotoxic against HCT-116 and MCF-7 human cancer cell lines (IC(50) = 10 μg/ml for both cancer cell lines).
authors: School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bangalore, 560065, India.
authors: Patel
authors: P
authors: Mohana Kumara
authors: Sebastian
authors: S
authors: Zuehlke
authors: Vaidyanathan
authors: V
authors: Priti
authors: Bheemanahally Thimmappa
authors: BT
authors: Ramesha
authors: Singh
authors: S
authors: Shweta
authors: Gudasalamani
authors: G
authors: Ravikanth
authors: Ramesh
authors: R
authors: Vasudeva
authors: Thankayyan Retnabai
authors: TR
authors: Santhoshkumar
authors: Michael
authors: M
authors: Spiteller
authors: Ramanan
authors: R
authors: Uma Shaanker
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D045744:Cell Line, Tumor; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D029582:Meliaceae / Q000382:microbiology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D024301:Plant Bark / Q000382:microbiology
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents