title: Molecular characterization of bovine placental and ovarian 20α-hydroxysteroid dehydrogenase.
abstract: The enzyme 20α-hydroxysteroid dehydrogenase (20α-HSD) catalyzes the conversion of progesterone to its inactive form, 20α-hydroxyprogesterone. This enzyme plays a critical role in the regulation of luteal function in female mammals. In this study, we conducted the characterization and functional analyses of bovine 20α-HSD from placental and ovarian tissues. The nucleotide sequence of bovine 20α-HSD showed significant homology to that of goats (96%), humans (84%), rabbits (83%), and mice (81%). The mRNA levels increased gradually throughout the estrous cycle, the highest being in the corpus luteum (CL) 1 stage. Northern blot analysis revealed a 1.2  kb mRNA in the bovine placental and ovarian tissues. An antibody specific to bovine 20α-HSD was generated in a rabbit immunized with the purified, recombinant protein. Recombinant 20α-HSD protein produced in mammalian cells had a molecular weight of ∼37  kDa. Bacterially expressed bovine 20α-HSD protein showed enzymatic activity. The expression pattern of the 20α-HSD protein in the pre-parturition placenta and the CL1 stage of the estrous cycle was similar to the level of 20α-HSD mRNA expression. Immunohistochemical analysis also revealed that bovine 20α-HSD protein was intensively localized in the large luteal cells during the late estrous cycle.
authors: Animal Biotechnology, Graduate School of Bio and Information Technology, Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Republic of Korea.
authors: Purevjargal
authors: P
authors: Naidansuren
authors: Cha-Won
authors: CW
authors: Park
authors: Sang-Hwan
authors: SH
authors: Kim
authors: Tseeleema
authors: T
authors: Nanjidsuren
authors: Jong-Ju
authors: JJ
authors: Park
authors: Seong-Jo
authors: SJ
authors: Yun
authors: Bo-Woong
authors: BW
authors: Sim
authors: Seongsoo
authors: S
authors: Hwang
authors: Myung-Hwa
authors: MH
authors: Kang
authors: Buom-Yong
authors: BY
authors: Ryu
authors: Sue-Yun
authors: SY
authors: Hwang
authors: Jong-Taek
authors: JT
authors: Yoon
authors: Keitaro
authors: K
authors: Yamanouchi
authors: Kwan-Sik
authors: KS
authors: Min
mesh_terms: D043607:20-alpha-Hydroxysteroid Dehydrogenase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D010802:Phylogeny; D010920:Placenta / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D043607:20-alpha-Hydroxysteroid Dehydrogenase