title: Molecular characterization and tissue distribution of ferritin M in kelp grouper, Epinephelus bruneus.
abstract: This study, reports the identification and analysis of ferritin M chain, from kelp grouper, Epinephelus bruneus (EbFerM); it comprises 1004 base pair (bp), including 528bp open reading frame (ORF) which encodes 176 amino acid (aa) residues; the calculated molecular weight is 20kDa. The 5'-untranslated region (UTR) possesses 476bp proceeded by a putative Iron Regulatory Element (IRE). Pair wise alignments showed that EbFerM shared 94% identity with that of Larimichthys crocea and Sciaenops. It is expressed in abundance in liver, spleen, and kidney when challenged with Vibrio anguillarum, lipopolysaccharide (LPS), or poly I:C.
authors: Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Sciences & Marine and Environmental Research Institute, Jeju National University, Jeju 690-756, South Korea.
authors: Ramasamy
authors: R
authors: Harikrishnan
authors: Ju-Sang
authors: JS
authors: Kim
authors: Ik-Soo
authors: IS
authors: Jang
authors: Man-Chul
authors: MC
authors: Kim
authors: Chellam
authors: C
authors: Balasundaram
authors: Moon-Soo
authors: MS
authors: Heo
mesh_terms: D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D005293:Ferritins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology* / Q000378:metabolism; D020869:Gene Expression Profiling; D008070:Lipopolysaccharides / Q000378:metabolism; D016366:Open Reading Frames; D009928:Organ Specificity; D010802:Phylogeny; D011070:Poly I-C / Q000378:metabolism; D016415:Sequence Alignment; D014733:Vibrio; D014735:Vibrio Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*
chemical_list: D018076:DNA, Complementary; D008070:Lipopolysaccharides; D005293:Ferritins; D011070:Poly I-C