title: Aldehyde dehydrogenase as an alternative to enumeration of total and viable CD34(+) cells in autologous hematopoietic progenitor cell transplantation.
abstract: We validated the correlation of aldehyde dehydrogenase ALDH(br) cells with total and viable CD34(+) cells in fresh and thawed hematopoietic progenitor cell (HPC) products, and looked for a correlation with time to white blood cell (WBC) and platelet engraftment after autologous transplantation, using simple linear regression analyzes. We found a significant correlation between pre-freeze ALDH(br) cell numbers and pre-freeze total CD34(+) (P < 0.001), viable CD34(+) (P < 0.001) and post-thaw viable CD34(+) (P < 0.001) cell numbers. We suggest that ALDH(br) may be substituted for CD34(+) cell numbers when evaluating HPC. As post-thaw viability testing apparently adds no significant information, we suggest that it may not be necessary. Finally, neither marker correlated with time to engraftment in our patients, supporting previous data suggesting the existence of a threshold dose for timely engraftment around 2.5 × 10(6) cells/kg.
authors: Myeloma Institute for Research and Therapy, University of Arkansas for Medical Sciences, Little Rock, USA.
authors: Muthu
authors: M
authors: Veeraputhiran
authors: Lakshmikanth
authors: L
authors: Katragadda
authors: Appathurai
authors: A
authors: Balamurugan
authors: Michele
authors: M
authors: Cottler-Fox
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002452:Cell Count / Q000379:methods; D002470:Cell Survival; D005240:Feasibility Studies; D006085:Graft Survival / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007108:Immune Tolerance; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D014182:Transplantation, Autologous
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D000444:Aldehyde Dehydrogenase