title: Synthesis of biocompatible PEG-Based star polymers with cationic and degradable core for siRNA delivery.
abstract: Star polymers with poly(ethylene glycol) (PEG) arms and a degradable cationic core were synthesized by the atom transfer radical copolymerization (ATRP) of poly(ethylene glycol) methyl ether methacrylate macromonomer (PEGMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), and a disulfide dimethacrylate (cross-linker, SS) via an "arm-first" approach. The star polymers had a diameter ~15 nm and were degraded under redox conditions by glutathione treatment into individual polymeric chains due to cleavage of the disulfide cross-linker, as confirmed by dynamic light scattering. The star polymers were cultured with mouse calvarial preosteoblast-like cells, embryonic day 1, subclone 4 (MC3T3-E1.4) to determine biocompatibility. Data suggest star polymers were biocompatible, with ≥ 80% cell viability after 48 h of incubation even at high concentration (800 μg/mL). Zeta potential values varied with N/P ratio confirming complexation with siRNA. Successful cellular uptake of the star polymers in MC3T3-E1.4 cells was observed by confocal microscopy and flow cytometry after 24 h of incubation.
authors: Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States.
authors: Hong Y
authors: HY
authors: Cho
authors: Abiraman
authors: A
authors: Srinivasan
authors: Joanna
authors: J
authors: Hong
authors: Eric
authors: E
authors: Hsu
authors: Shiguang
authors: S
authors: Liu
authors: Arun
authors: A
authors: Shrivats
authors: Dan
authors: D
authors: Kwak
authors: Andrew K
authors: AK
authors: Bohaty
authors: Hyun-Jong
authors: HJ
authors: Paik
authors: Jeffrey O
authors: JO
authors: Hollinger
authors: Krzysztof
authors: K
authors: Matyjaszewski
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis / Q000138:chemical synthesis* / Q000494:pharmacology; D002412:Cations / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000032:analysis / Q000138:chemical synthesis* / Q000494:pharmacology; D004705:Endocytosis; D005434:Flow Cytometry; D005978:Glutathione / Q000378:metabolism; D008027:Light; D008689:Methacrylates / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000737:chemistry / Q000378:metabolism; D012542:Scattering, Radiation
chemical_list: D001672:Biocompatible Materials; D002412:Cations; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D008689:Methacrylates; D034741:RNA, Small Interfering; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D005978:Glutathione; C049840:2-(dimethylamino)ethyl methacrylate