title: Biological pretreatment with a cellobiose dehydrogenase-deficient strain of Trametes versicolor enhances the biofuel potential of canola straw.
abstract: The use of Trametes versicolor as a biological pretreatment for canola straw was explored in the context of biofuel production. Specifically, the effects on the straw of a wild-type strain (52J) and a cellobiose dehydrogenase (CDH)-deficient strain (m4D) were investigated. The xylose and glucose contents of the straw treated with 52J were significantly reduced, while only the xylose content was reduced with m4D treatment. Lignin extractability was greatly improved with fungal treatments compared to untreated straw. Saccharification of the residue of the m4D-treated straw led to a significant increase in proportional glucose yield, which was partially attributed to the lack of cellulose catabolism by m4D. Overall, the results of this study indicate that CDH facilitates cellulose access by T. versicolor. Furthermore, treatment of lignocellulosic material with m4D offers improvements in lignin extractability and saccharification efficacy compared to untreated biomass without loss of substrate due to fungal catabolism.
authors: Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, Canada.
authors: Thomas
authors: T
authors: Canam
authors: Jennifer R
authors: JR
authors: Town
authors: Adrian
authors: A
authors: Tsang
authors: Tim A
authors: TA
authors: McAllister
authors: Tim J
authors: TJ
authors: Dumonceaux
mesh_terms: D000143:Acids / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D029688:Brassica napus / Q000737:chemistry* / Q000187:drug effects; D002237:Carbohydrate Dehydrogenases / Q000037:antagonists & inhibitors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002473:Cell Wall / Q000378:metabolism; D004875:Ergosterol / Q000032:analysis; D005285:Fermentation / Q000187:drug effects; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D012995:Solubility / Q000187:drug effects; D013997:Time Factors; D055454:Trametes / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014866:Waste Products / Q000032:analysis*
chemical_list: D000143:Acids; D000447:Aldehydes; D056804:Biofuels; D014866:Waste Products; C008046:5-hydroxymethylfurfural; D008031:Lignin; D005662:Furaldehyde; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D005947:Glucose; D004875:Ergosterol