title: On the benefit of whey-cultured Lactobacillus casei in murine colitis.
abstract: The objective of this study was to examine the prophylactic and therapeutic effect of whey-cultured Lactobacillus casei (L. casei) in a murine model of colitis. Colitis was induced by intracolonic administration of a mixture of 2,4,6-trinitrobenzenesulphonic acid (TNBS)/absolute ethanol in male Wistar rats. Animals were divided into 5 groups including sham (normal group), control (vehicle-treated), positive control (dexamethasone 1 mg/kg/day, orally), prevention (10(8) cfu L. casei/day, orally, 14 days before induction of colitis), and treatment (10(8) cfu L. casei/day, orally, 14 days after induction of colitis). After 14-days treatment, the animals were sacrificed on the day 15. Distal colons were removed for examining histological and biochemical assays. Biomarkers including TNF-α, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the homogenate of colon. Results indicated an apparent improvement in colon histopathology scores, TNF-α, MPO, and LPO in the treatment group, whereas prevention group did not demonstrate positive efficacy in prevention of colonic damage. It is concluded that L. casei grown in whey culture is very effective in ameliorating both biochemical and histopathological markers of colitis if used post induction of colitis but not if used before induction of colitis. The difference between effects of L. casei when used pre-colitis and post-colitis confirms its mechanism of action as an anti toxic stress agent. Further studies should be made in IBD patients.
authors: Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors: S F
authors: SF
authors: Ghasemi-Niri
authors: A H
authors: AH
authors: Abdolghaffari
authors: S
authors: S
authors: Fallah-Benakohal
authors: M
authors: M
authors: Hosseinpour-Feizi
authors: P
authors: P
authors: Mahdaviani
authors: H
authors: H
authors: Jamalifar
authors: M
authors: M
authors: Baeeri
authors: G
authors: G
authors: Dehghan
authors: M
authors: M
authors: Abdollahi
mesh_terms: D000285:Administration, Rectal; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy*; D003106:Colon / Q000473:pathology*; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage; D007780:Lacticaseibacillus casei; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D008894:Milk Proteins / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D019936:Probiotics / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000067816:Whey Proteins
chemical_list: D000893:Anti-Inflammatory Agents; D008894:Milk Proteins; D014409:Tumor Necrosis Factor-alpha; D000067816:Whey Proteins; D000431:Ethanol; D003907:Dexamethasone; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase