title: Genus-specific recruitment of filovirus ribonucleoprotein complexes into budding particles.
abstract: The filoviral matrix protein VP40 orchestrates virus morphogenesis and budding. To do this it interacts with both the glycoprotein (GP1,2) and the ribonucleoprotein (RNP) complex components; however, these interactions are still not well understood. Here we show that for efficient VP40-driven formation of transcription and replication-competent virus-like particles (trVLPs), which contain both an RNP complex and GP1,2, the RNP components and VP40, but not GP1,2 and VP40, must be from the same genus. trVLP preparations contained both spherical and filamentous particles, but only the latter were able to infect target cells and to lead to genome replication and transcription. Interestingly, the genus specificity of the VP40-RNP interactions was specific to the formation of filamentous trVLPs, but not to spherical particles. These results not only further our understanding of VP40 interactions, but also suggest that special care is required when using trVLP or VLP systems to model virus morphogenesis.
authors: Department of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors: Larissa
authors: L
authors: Spiegelberg
authors: Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, MD, USA.
authors: Victoria
authors: V
authors: Wahl-Jensen
authors: Department of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors: Larissa
authors: L
authors: Kolesnikova
authors: Laboratory of Virology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
authors: Heinz
authors: H
authors: Feldmann
authors: Department of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors: Stephan
authors: S
authors: Becker
authors: Laboratory of Virology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.|Department of Virology, Philipps University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany.
authors: Thomas
authors: T
authors: Hoenen
mesh_terms: D029043:Ebolavirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D029024:Marburgvirus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014763:Viral Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D012261:Ribonucleoproteins; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins