title: Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip.
abstract: The objective of this paper is to introduce a novel cell printing and microfluidic system to serve as a portable ground model for the study of drug conversion and radiation protection of living liver tissue analogs. The system is applied to study behavior in ground models of space stress, particularly radiation. A microfluidic environment is engineered by two cell types to prepare an improved higher fidelity in vitro micro-liver tissue analog. Cell-laden Matrigel printing and microfluidic chips were used to test radiation shielding to liver cells by the pro-drug amifostine. In this work, the sealed microfluidic chip regulates three variables of interest: radiation exposure, anti-radiation drug treatment and single- or dual-tissue culture environments. This application is intended to obtain a scientific understanding of the response of the multi-cellular biological system for long-term manned space exploration, disease models and biosensors.
authors: Department of Mechanical Engineering, Drexel University, Philadelphia, PA 19104, USA.
authors: J E
authors: JE
authors: Snyder
authors: Q
authors: Q
authors: Hamid
authors: C
authors: C
authors: Wang
authors: R
authors: R
authors: Chang
authors: K
authors: K
authors: Emami
authors: H
authors: H
authors: Wu
authors: W
authors: W
authors: Sun
mesh_terms: D004999:Amifostine / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival; D018920:Coculture Techniques; D003094:Collagen / Q000737:chemistry*; D004338:Drug Combinations; D005720:Gamma Rays; D022781:Hepatocytes / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011355:Prodrugs / Q000378:metabolism*; D011509:Proteoglycans / Q000737:chemistry*; D013696:Temperature
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011355:Prodrugs; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D004999:Amifostine