title: Roles of hinge region, loops 3 and 4 in the activation of Escherichia coli cyclic AMP receptor protein.
abstract: The cAMP receptor protein (CRP) requires cAMP for an allosteric change and regulates more than 150 genes in Escherichia coli. In this study, the modular half of cAMP receptor protein was used to investigate the allosteric signal transmission pathway induced by cAMP binding. The activation of CRP upon cAMP binding is indicated to be realignment of the two subunits within the CRP dimer. The interaction of loop 3 and Phe136 do not involve in signal transmission.
authors: Department of Chemistry, Fudan University, Shanghai 200433, China.
authors: Zhengya
authors: Z
authors: Gao
authors: Feng
authors: F
authors: Li
authors: Guangrong
authors: G
authors: Wu
authors: Yanrun
authors: Y
authors: Zhu
authors: Ting
authors: T
authors: Yu
authors: Shaoning
authors: S
authors: Yu
mesh_terms: D020816:Amino Acid Motifs; D002151:Calorimetry / Q000379:methods; D002918:Chymotrypsin / Q000737:chemistry; D002942:Circular Dichroism; D002373:Cyclic AMP Receptor Protein / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry; D007700:Kinetics; D046911:Macromolecular Substances / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D013050:Spectrometry, Fluorescence / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature
chemical_list: D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; D046911:Macromolecular Substances; D002918:Chymotrypsin