title: Transmembrane helices can induce domain formation in crowded model membranes.
abstract: We studied compositionally heterogeneous multi-component model membranes comprised of saturated lipids, unsaturated lipids, cholesterol, and α-helical TM protein models using coarse-grained molecular dynamics simulations. Reducing the mismatch between the length of the saturated and unsaturated lipid tails reduced the driving force for segregation into liquid-ordered (l(o)) and liquid-disordered (l(d)) lipid domains. Cholesterol depletion had a similar effect, and binary lipid mixtures without cholesterol did not undergo large-scale phase separation under the simulation conditions. The phase-separating ternary dipalmitoyl-phosphatidylcholine (DPPC)/dilinoleoyl-PC (DLiPC)/cholesterol bilayer was found to segregate into l(o) and l(d) domains also in the presence of a high concentration of ΤΜ helices. The l(d) domain was highly crowded with TM helices (protein-to-lipid ratio ~1:5), slowing down lateral diffusion by a factor of 5-10 as compared to the dilute case, with anomalous (sub)-diffusion on the μs time scale. The membrane with the less strongly unsaturated palmitoyl-linoleoyl-PC instead of DLiPC, which in the absence of TM α-helices less strongly deviated from ideal mixing, could be brought closer to a miscibility critical point by introducing a high concentration of TM helices. Finally, the 7-TM protein bacteriorhodopsin was found to partition into the l(d) domains irrespective of hydrophobic matching. These results show that it is possible to directly study the lateral reorganization of lipids and proteins in compositionally heterogeneous and crowded model biomembranes with coarse-grained molecular dynamics simulations, a step toward simulations of realistic, compositionally complex cellular membranes. This article is part of a Special Issue entitled: Protein Folding in Membranes.
authors: Groningen Biomolecular Sciences and Biotechnology Institute, and Zernike Institute for Advanced Materials, University of Groningen, Nijemborgh 7, 9747 AG Groningen, The Netherlands.
authors: Jan
authors: J
authors: Domański
authors: Siewert J
authors: SJ
authors: Marrink
authors: Lars V
authors: LV
authors: Schäfer
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D004058:Diffusion; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D008954:Models, Biological; D056004:Molecular Dynamics Simulation; D044367:Phase Transition; D010713:Phosphatidylcholines / Q000737:chemistry; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D005231:Fatty Acids, Unsaturated; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D001436:Bacteriorhodopsins; C002448:1,2-linoleoylphosphatidylcholine; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine