title: [Observation of genetic diversity in dental plaque of elder people with root caries].
abstract: OBJECTIVE
  Bacterial community in dental plaque of elder people was analyzed to learn about the microhabitat composition and diversity.
  
  
  METHODS
  Dental plaque samples were collected from 25 elders. PCR-based denaturing gradient gel electrophoresis (PCR-DGGE) was used to evaluate the microbial diversity by displaying PCR-generated 16SrDNA fragments that migrate at different distances, reflecting the different sequence of fragment. SPSS12.0 software was used to analyze the variance of genotypes between different groups of bacteria.
  
  
  RESULTS
  Genotypes of bacteria in dental plaques in the root caries group was significantly more than the other two groups. Crown caries group and caries-free group had no significant difference.
  
  
  CONCLUSIONS
  The genetic diversity of the dental plaque microflora in the root caries group is significantly higher than coronal caries group and caries-free group.
authors: Department of Endodontics and Operative Dentistry, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. mashanfen@yahoo.com.cn
authors: Shan-fen
authors: SF
authors: Ma
authors: Jing-ping
authors: JP
authors: Liang
authors: Yun-tao
authors: YT
authors: Jiang
authors: Cai-lian
authors: CL
authors: Zhu
mesh_terms: D000368:Aged; D001419:Bacteria; D004269:DNA, Bacterial; D058645:Denaturing Gradient Gel Electrophoresis; D003731:Dental Caries; D003773:Dental Plaque; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D017213:Root Caries
chemical_list: D004269:DNA, Bacterial