title: Counter-ionic effect on the separation of water-soluble compounds applying a hydrophilic stationary phase bonded with a zwitter-ionic polymer.
abstract: A novel hydrophilic stationary phase bonded with a zwitter-ionic polymer for HPLC was synthesized. The stationary phase, in combination with a mobile phase containing various salts, was evaluated for its ability to separate water-soluble compounds, such as nucleobases, nucleosides and glycosides. The retention of a large majority of the solutes, except for cytosine, was increased by adding anti-chaotropic ions to the mobile phase. These results suggested that the retention of solutes depended on the thickness of the hydration layer on the stationary phase. In the zwitter-ionic polymer adsorbent, the formation of the hydration layer and the ionicity of the zwitter-ionic group on the stationary phase will be controlled by the properties of the ions added to the mobile phase.
authors: Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi, Tokyo 208-8577, Japan. w-kamichatani@filcon.co.jp
authors: Waka
authors: W
authors: Kamichatani
authors: Yoshinori
authors: Y
authors: Inoue
authors: Mitsuru
authors: M
authors: Saito
authors: Yasuyuki
authors: Y
authors: Kobayashi
authors: Atsushi
authors: A
authors: Yamamoto
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012492:Salts / Q000737:chemistry; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D006027:Glycosides; D007477:Ions; D009696:Nucleic Acids; D009705:Nucleosides; D011108:Polymers; D012492:Salts; D012997:Solvents; D014867:Water