title: On-column solvent exchange for purified preparative fractions.
abstract: On-column solvent exchange, using many of the principles of solid-phase extraction, has been implemented to significantly reduce evaporation cycle time following reverse-phase preparative HPLC. Additional benefits, such as a reduced potential for salt formation, thermal decomposition, and residual solvent, are also described. Fractions obtained from preparative separations, typically in a large volume of acetonitrile:water, are injected into the preparative HPLC and then eluted in acetonitrile, creating a new fraction in a volatile organic solvent. Minimal modification to the instrument was required, and unattended operation is possible. Acetonitrile evaporation is achieved within 3 h, compared with 17 h for aqueous-based fractions; lower temperatures can be used during the evaporation step; mobile-phase additives, likely to form salts with the target compound if concentrated in the fraction, are removed before evaporation; sample recovery and purity are unaffected.
authors: Pfizer Global Research and Development, Analytical Chemistry and Sample Logistics, St Louis Laboratories, Chesterfield, MO, USA. gilles.h.goetz@pfizer.com
authors: Gilles H
authors: GH
authors: Goetz
authors: Emily
authors: E
authors: Beck
authors: Peter W
authors: PW
authors: Tidswell
mesh_terms: D000097:Acetonitriles / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012997:Solvents / Q000737:chemistry*; D014835:Volatilization
chemical_list: D000097:Acetonitriles; D012997:Solvents; C032159:acetonitrile