title: [Influence of angiotensin-(1-7) on angiotensin II induced rat's tubular epithelial-myofibroblast transdifferentiation].
abstract: OBJECTIVE
  To explore the influence of angiotensin-(1-7)[Ang-(1-7)] on angiotension II(Ang II) induced rat's tubular epithelial-myofibroblast transdifferentiation and the secretion of extracellular matrix.
  
  
  METHODS
  The NRK52E were maintained and sub-cultured treated with Ang-(1-7) (10(-6); mmol/L) and Ang II(10(-6); mmol/L) for 24, 48, 72, 96 hours, we detect the protein expressions of E-cadherin and α-SMA by immunocytochemistry method; The content of Col I and FN in the cultured supernatant were measured by ELISA; The mRNA expression of E-cadherin, α-SMA, Col I and FN was detected by real-time PCR.
  
  
  RESULTS
  Treat with ang II 96 h, the protein and mRNA expression of E-cadherin decreased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN increased significantly (P<0.05); treat with Ang II and Ang-(1-7), the protein and mRNA expression of E-cadherin increased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN decreased significantly (P<0.05).
  
  
  CONCLUSIONS
  Ang-(1-7) can inhibits Ang II-induced rat's tubular epithelial myofibroblast transdifferentiation and decrease the secretion of FN and Col I.
authors: Department of Nephrology, Luzhou Medical College, Luzhou 646000, China. yuanboyanjiusheng@163.com
authors: Bo
authors: B
authors: Yuan
authors: Hong-Yan
authors: HY
authors: He
authors: Ming-Yong
authors: MY
authors: Wang
authors: Jian
authors: J
authors: Liu
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000803:Angiotensin I / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects* / Q000235:genetics; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007684:Kidney Tubules / Q000187:drug effects* / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D058628:Myofibroblasts / Q000187:drug effects* / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
chemical_list: D000199:Actins; D000803:Angiotensin I; D000804:Angiotensin II; D015820:Cadherins; D005353:Fibronectins; D020778:Matrix Metalloproteinase 2; D010446:Peptide Fragments; D012333:RNA, Messenger; C498862:Acta2 protein, rat; C118790:angiotensin I (1-7)