title: Muscle transcriptomic analyses in Angus cattle with divergent tenderness.
abstract: Beef tenderness contributes significantly to variation of beef palatability, and is largely influenced by various genetic and environmental factors. To identify candidate genes and pathways related to beef tenderness, we analyzed the longissimus dorsi (LD) muscle of Angus cattle that had different degrees of tenderness, measured by Warner-Bratzler shear force (WBSF). Microarray and RT-PCR analyses identified 53 genes that were differentially expressed in LD samples categorized as either tough or tender, including myosin, heavy chain 3 skeletal muscle embryonic (MYH3), myosin heavy chain 8 skeletal muscle perinatal (MYH8), guanylate binding protein 5 (GBP5), fatty acid binding protein 4 (FABP4), Stearoyl-coenzyme A desaturase (SCD), Fatty acid synthase (FASN), ubiquitin-like with PHD and ring finger domains 1 (UHRF1). Most of these genes are involved in lipid metabolism and skeletal muscle contraction. Employing Gene ontology (GO) and Ingenuity Pathway Analysis (IPA), several GO terms and pathways were found to be related to hydrolase, peptidase and GTPase activity, lipid metabolism, small molecule biochemistry, molecular transport, and tissue development. Overall, this analysis provides insight into the metabolic relationships between muscle biology and beef quality.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors: Chunping
authors: C
authors: Zhao
authors: Fei
authors: F
authors: Tian
authors: Ying
authors: Y
authors: Yu
authors: Juan
authors: J
authors: Luo
authors: Qiong
authors: Q
authors: Hu
authors: Brian J
authors: BJ
authors: Bequette
authors: Ransom L
authors: RL
authors: Baldwin Vi
authors: George
authors: G
authors: Liu
authors: Linsen
authors: L
authors: Zan
authors: M
authors: M
authors: Scott Updike
authors: Jiuzhou
authors: J
authors: Song
mesh_terms: D050154:Adiposity / Q000235:genetics; D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks / Q000235:genetics; D008460:Meat / Q000032:analysis*; D058977:Molecular Sequence Annotation; D018482:Muscle, Skeletal / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D005227:Fatty Acids