title: A reduced-function allele reveals that EARLY FLOWERING3 repressive action on the circadian clock is modulated by phytochrome signals in Arabidopsis.
abstract: Arabidopsis thaliana EARLY FLOWERING3 (ELF3) is essential for the generation of circadian rhythms. ELF3 has been proposed to restrict light signals to the oscillator through phytochrome photoreceptors, but that has not been explicitly shown. Furthermore, the genetic action of ELF3 within the clock had remained elusive. Here, we report a functional characterization of ELF3 through the analysis of the elf3-12 allele, which encodes an amino acid replacement in a conserved domain. Circadian oscillations persisted, and unlike elf3 null alleles, elf3-12 resulted in a short circadian period only under ambient light. The period shortening effect of elf3-12 was enhanced by the overexpression of phytochromes phyA and phyB. We found that elf3-12 was only modestly perturbed in resetting of the oscillator and in gating light-regulated gene expression. Furthermore, elf3-12 essentially displayed wild-type development. We identified targets of ELF3 transcriptional repression in the oscillator, highlighting the action at the morning gene PSEUDO-RESPONSE REGULATOR9. Taken together, we identified two separable roles for ELF3, one affecting the circadian network and the other affecting light input to the oscillator. This is consistent with a dual function of ELF3 as both an integrator of phytochrome signals and a repressor component of the core oscillator.
authors: Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
authors: Elsebeth
authors: E
authors: Kolmos
authors: Eva
authors: E
authors: Herrero
authors: Nora
authors: N
authors: Bujdoso
authors: Andrew J
authors: AJ
authors: Millar
authors: Réka
authors: R
authors: Tóth
authors: Peter
authors: P
authors: Gyula
authors: Ferenc
authors: F
authors: Nagy
authors: Seth J
authors: SJ
authors: Davis
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D057906:Circadian Clocks; D018506:Gene Expression Regulation, Plant; D008027:Light; D051942:Phytochrome A / Q000235:genetics / Q000378:metabolism*; D051939:Phytochrome B / Q000235:genetics / Q000378:metabolism*; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D051942:Phytochrome A; D051939:Phytochrome B; D018749:RNA, Plant; D014157:Transcription Factors; C432430:At2g25930 protein, Arabidopsis