title: Enzymatic treatment and detoxification of acid orange 7 from textile wastewater.
abstract: A crude preparation of horseradish roots was used as a low-purity source of horseradish peroxidase (HRP) in dye decolorization experiments. The technical feasibility of the process was studied in bench scale for enzymatic removal of acid orange 7 (AO7), a synthetic dye. Further studies were carried out to understand the effects of process parameters such as pH value, H(2)O(2) level, concentrations of the synthetic dye, and HRP during enzyme-mediated dye degradation. Experimental data revealed that the concentration of AO7, pH of the aqueous phase, amount of the enzyme, and H(2)O(2) level played significant roles on the overall enzymatic reaction. Polyethylene glycol, as an anti-inactivation of HRP, in various concentrations showed no significant effect on the decolorization. The experimental data of initial reaction rates were fitted using an analytical equation proposed by Michaelis-Menten. The acute toxicity tests using Daphnia magna exhibited that the enzymatic treatment significantly decreased the toxicity of the dye solution.
authors: School of Public Health and Center for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran.
authors: Fathollah
authors: F
authors: Gholami-Borujeni
authors: Amir Hossein
authors: AH
authors: Mahvi
authors: Simin
authors: S
authors: Nasseri
authors: Mohammad Ali
authors: MA
authors: Faramarzi
authors: Ramin
authors: R
authors: Nabizadeh
authors: Mahmood
authors: M
authors: Alimohammadi
mesh_terms: D000818:Animals; D031215:Armoracia / Q000737:chemistry / Q000201:enzymology*; D001391:Azo Compounds / Q000737:chemistry* / Q000633:toxicity; D001557:Benzenesulfonates / Q000737:chemistry* / Q000633:toxicity; D004396:Coloring Agents / Q000737:chemistry* / Q000633:toxicity; D003621:Daphnia / Q000187:drug effects; D052918:Environmental Restoration and Remediation / Q000379:methods*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D012722:Sewage / Q000009:adverse effects / Q000737:chemistry*; D013784:Textiles / Q000009:adverse effects
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D010940:Plant Proteins; D012722:Sewage; D006735:Horseradish Peroxidase; C014722:2-naphthol orange