title: Pentraxin 3 as a potential biomarker of acetaminophen-induced liver injury.
abstract: OBJECTIVE
  Overdose of acetaminophen (APAP) can lead to severe liver injury in humans and experimental animals. Pentraxin-3 (PTX-3) is produced and released by several cell types. In this study, we aimed to evaluate whether PTX-3 is a potential biomarker in the identification of APAP-induced liver injury.
  
  
  METHODS
  Thirty adult male Wistar rats were randomly divided into three groups: control, APAP-1 and APAP-2 groups. APAP-1 (1 g/kg) and APAP-2 (2 g/kg) group rats were given APAP by gastric tube. Liver tissues and blood samples were obtained for biochemical and histopathological analysis. Biochemical parameters, plasma and liver PTX-3 levels and degree of liver necrosis were measured in all groups.
  
  
  RESULTS
  APAP treatments caused necrosis in liver and accompanied by elevated liver PTX-3 levels after 48 h. In APAP-1 and APAP-2 groups when compared with control group (7.5±3.3 ng/mg protein), mean liver PTX-3 concentrations were 14.1±3.0 (p=0.032) and 28.5±8.2 (p<0.001) ng/mg protein, respectively. All rats (100%) in the APAP-2 group had the degree 3 liver necrosis. However 10%, 40% and 50% of rats had the degree 1, the degree 2 and the degree 3 liver necrosis in the APAP-1 group, respectively. The degrees of liver necrosis of the APAP-1 and APAP-2 groups were higher than the group of control (p<0.001 and p<0.001, respectively).
  
  
  CONCLUSIONS
  PTX-3 may have a role in the APAP-induced liver injury in the rats. The elevated liver PTX-3 in the APAP-induced hepatic necrosis might be a marker of acute histological liver damage. Further prospective studies are necessary to clarify the prognostic value of liver PTX-3 for prediction of histological hepatic necrosis in the APAP-induced liver injury.
authors: Department of Medical Biochemistry, Gulhane Military Medical Academy, Etlik-06018 Ankara, Turkey. hyaman@gata.edu.tr
authors: Halil
authors: H
authors: Yaman
authors: Erdinc
authors: E
authors: Cakir
authors: Emin O
authors: EO
authors: Akgul
authors: Ibrahim
authors: I
authors: Aydin
authors: Onder
authors: O
authors: Onguru
authors: Tuncer
authors: T
authors: Cayci
authors: Yasemin Gulcan
authors: YG
authors: Kurt
authors: Mehmet
authors: M
authors: Agilli
authors: Fevzi N
authors: FN
authors: Aydin
authors: Mustafa
authors: M
authors: Gulec
authors: Ozcan
authors: O
authors: Altinel
authors: Salim
authors: S
authors: Isbilir
authors: Nail
authors: N
authors: Ersoz
authors: Mehmet
authors: M
authors: Yasar
authors: Turker
authors: T
authors: Turker
authors: Cumhur
authors: C
authors: Bilgi
authors: Kemal M
authors: KM
authors: Erbil
mesh_terms: D000082:Acetaminophen / Q000633:toxicity*; D000284:Administration, Oral; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002097:C-Reactive Protein / Q000032:analysis* / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009336:Necrosis; D051381:Rats; D017208:Rats, Wistar; D000683:Serum Amyloid P-Component / Q000032:analysis* / Q000378:metabolism; D000099153:Pentraxins
chemical_list: D000082:Acetaminophen; D018712:Analgesics, Non-Narcotic; D015415:Biomarkers; D002097:C-Reactive Protein; D000683:Serum Amyloid P-Component; D000099153:Pentraxins