title: Characterization of a mutant strain of a filamentous fungus Cladosporium phlei for the mass production of the secondary metabolite phleichrome.
abstract: UV-mutagenesis was performed to obtain mutant strains that demonstrate altered production of phleichrome, a secondary metabolite of Cladosporium phlei. Among fifty mutants selected, based on the increased area and intensity of the purple pigment surrounding the colonies, the strain M0035 showed the highest production of phleichrome, more than seven fold over wild type. Plate cultures of the M0035 strain resulted in a total of 592 mg phleichrome consisting of 146 mg and 446 mg from the mycelia and agar media, respectively. The M0035 strain displayed a growth rate and a mycelial mass comparable to the parental strain but had significantly reduced asexual sporulation.
authors: Institute for Molecular Biology and Genetics, Center for Fungal Pathogenesis, Chonbuk National University, Chonbuk 561-756, Republic of Korea.
authors: Min-Hee
authors: MH
authors: Yi
authors: Jung-Ae
authors: JA
authors: Kim
authors: Jung-Mi
authors: JM
authors: Kim
authors: Jin-Ah
authors: JA
authors: Park
authors: Beom-Tae
authors: BT
authors: Kim
authors: Seung-Moon
authors: SM
authors: Park
authors: Moon-Sik
authors: MS
authors: Yang
authors: Ki-Jun
authors: KJ
authors: Hwang
authors: Dae-Hyuk
authors: DH
authors: Kim
mesh_terms: D002963:Cladosporium / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D009154:Mutation; D009281:Naphthalenes / Q000378:metabolism*; D010641:Phenotype; D014466:Ultraviolet Rays
chemical_list: D009281:Naphthalenes; C061463:calphostin D