title: Molecular features of secondary vascular tissue regeneration after bark girdling in Populus.
abstract: Regeneration is a common strategy for plants to repair damage to their tissue after attacks from other organisms or physical assaults. However, how differentiating cells acquire regenerative competence and rebuild the pattern of new tissues remains largely unknown. Using anatomical observation and microarray analysis, we investigated the morphological process and molecular features of secondary vascular tissue regeneration after bark girdling in trees. After bark girdling, new phloem and cambium regenerate from differentiating xylem cells and rebuild secondary vascular tissue pattern within 1 month. Differentiating xylem cells acquire regenerative competence through epigenetic regulation and cell cycle re-entry. The xylem developmental program was blocked, whereas the phloem or cambium program was activated, resulting in the secondary vascular tissue pattern re-establishment. Phytohormones play important roles in vascular tissue regeneration. We propose a model describing the molecular features of secondary vascular tissue regeneration after bark girdling in trees. It provides information for understanding mechanisms of tissue regeneration and pattern formation of the secondary vascular tissues in plants.
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors: Jing
authors: J
authors: Zhang
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors: Ge
authors: G
authors: Gao
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors: Jia-Jia
authors: JJ
authors: Chen
authors: School of Biological Sciences, University of Southampton SO16 7PX, UK.
authors: Gail
authors: G
authors: Taylor
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors: Ke-Ming
authors: KM
authors: Cui
authors: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors: Xin-Qiang
authors: XQ
authors: He
mesh_terms: D058506:Cambium / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D044127:Epigenesis, Genetic; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D008954:Models, Biological; D052585:Phloem / Q000235:genetics; D024301:Plant Bark / Q000254:growth & development / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism; D058526:Plant Vascular Bundle / Q000254:growth & development / Q000502:physiology*; D032107:Populus / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics; D052584:Xylem / Q000166:cytology / Q000235:genetics
chemical_list: D010937:Plant Growth Regulators; D012333:RNA, Messenger; D014157:Transcription Factors