title: Estrogen receptor beta does not influence ischemic tolerance in the aged female rat heart.
abstract: BACKGROUND
  Ischemic heart disease remains the leading cause of morbidity and mortality in aged women, with a 2- to 3-fold increase in incidence following menopause. Clinical trials have failed to demonstrate cardioprotective benefit from chronic estrogen (E(2)) replacement therapy, yet protective effects of E(2) have been demonstrated in adult animal models and are mediated by the estrogen receptor (ER) subtypes ERα and ERβ.
  
  
  OBJECTIVE
  The aim of this study was to determine the effects of acute ERβ activation on ischemia/reperfusion (I/R) injury in adult, aged, and aged E(2)-deficient female rats.
  
  
  METHODS
  Hearts were isolated from adult (6 months; n = 9), aged (24 months; n = 13), and aged ovariectomized (OVX; n = 14) female Fischer 344 rats and subjected to 47 min of global I and 60 min of R. Rats were acutely treated with the ERβ-agonist diarylpropionitrile (DPN; 5 μg/kg) or vehicle 45 min prior to I/R; ERβ mRNA and protein levels were also assessed.
  
  
  RESULTS
  Acute treatment with DPN had no effect on functional recovery following I/R injury in adult, aged, or aged OVX female rats. Additionally, we were unable to detect ERβ mRNA or protein in the adult or aged female rat myocardium.
  
  
  CONCLUSIONS
  Here, for the first time, our data suggest that acute ERβ activation does not impact ischemic tolerance in the adult or aged female Fischer 344 rat myocardium and this likely due to a lack of detectable ERβ.
authors: Intercollege Graduate Degree Program in Physiology, The Pennsylvania State University, University Park, PA 16802, USA.
authors: Nanette J
authors: NJ
authors: Tomicek
authors: Jennifer L
authors: JL
authors: Miller-Lee
authors: J Craig
authors: JC
authors: Hunter
authors: Donna H
authors: DH
authors: Korzick
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D004958:Estradiol / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D010052:Ovariectomy; D011422:Propionates / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D020127:Recovery of Function; D013997:Time Factors; D014162:Transfection; D016277:Ventricular Function, Left; D017725:Ventricular Pressure
chemical_list: C486182:2,3-bis(4-hydroxyphenyl)-propionitrile; D047629:Estrogen Receptor beta; D009570:Nitriles; D011422:Propionates; D012333:RNA, Messenger; D004958:Estradiol