title: Practical tip: Chicago Sky Blue (CSB) stain can be added to the routine potassium hydroxide (KOH) wet-mount to provide a color contrast and facilitate the diagnosis of dermatomycoses.
abstract: Rapid confirmation of dermatomycoses is desirable because it allows the clinician to initiate appropriate therapy without delay. The routine potassium hydroxide (KOH) wet-mount is cheap and rapid to use but this method lacks a color contrast. We offer a simple practical tip of adding Chicago Sky Blue (CSB) stain to KOH to highlight fungal elements and provide a color contrast that makes reading and interpretation simple, even for the novice.
authors: Central Manpower Base, Ministry of Defence, Singapore, Singapore.
authors: Christopher Seng-Hong
authors: CS
authors: Lim
authors: Siew-Lin
authors: SL
authors: Lim
mesh_terms: D004396:Coloring Agents / Q000008:administration & dosage; D003881:Dermatomycoses / Q000175:diagnosis*; D006801:Humans; D006878:Hydroxides; D017680:Potassium Compounds; D013194:Staining and Labeling; D014343:Trypan Blue / Q000008:administration & dosage
chemical_list: D004396:Coloring Agents; D006878:Hydroxides; D017680:Potassium Compounds; C009000:pontamine sky blue; D014343:Trypan Blue; C029943:potassium hydroxide