title: Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair.
abstract: The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals were positive for the virus by qRT-PCR performed on the whole blood and when samples of more than 30 hairs were assayed. Additionally, qRT-PCR was performed on groups of 10 and 20 hairs harvested from 7 out of 23 immunohistochemical staining-positive calves. When groups of 20 and 10 hairs were tested, 6 and 4 animals, respectively, were positive for the virus.
authors: Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL 61802, USA. ksingh08@illinois.edu
authors: Kuldeep
authors: K
authors: Singh
authors: Myrna M
authors: MM
authors: Miller
authors: Laura J
authors: LJ
authors: Kohrt
authors: Gail
authors: G
authors: Scherba
authors: Edgar F
authors: EF
authors: Garrett
authors: Richard L
authors: RL
authors: Fredrickson
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D030242:Diarrhea Virus 2, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D006197:Hair / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral