title: Novel tripeptides with α-glucosidase inhibitory activity isolated from silk cocoon hydrolysate.
abstract: Active compounds with antidiabetic potential were isolated from silk peptide E5K6 by consecutive ultrafiltration and gel filtration using Biogel P-2 and RS-HPLC using a YMC-Pack Pro C18 column. The highest α-glucosidase inhibitory activity of silk peptide E5K6 resulted from fractions with MW <1 kDa. The activities of gel-filtered fractions from silk peptide E5K6 of <1 kDa were assayed in vitro, demonstrating that the fourth peak (F4) had the highest α-glucosidase inhibitory activity (IC(50) = 37.1 mg/mL). F4 of silk peptide E5K6 was separated by HPLC into two peaks. Moreover, the purified compounds were identified as Gly-Glu-Tyr (GEY, MW = 367 Da) and Gly-Tyr-Gly (GYG, MW = 295 Da) according to amino acid sequences, and their α-glucosidase inhibitory activities (IC(50)) were 2.7 and 1.5 mg/mL, respectively.
authors: Department of Food and Nutrition, Korea University, Seoul 130-701, Republic of Korea.
authors: Hyun Jung
authors: HJ
authors: Lee
authors: Hyun-Sun
authors: HS
authors: Lee
authors: Jang Won
authors: JW
authors: Choi
authors: Kyung Soo
authors: KS
authors: Ra
authors: Jin-Man
authors: JM
authors: Kim
authors: Hyung Joo
authors: HJ
authors: Suh
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D006868:Hydrolysis; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D047011:Silk / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000032:analysis
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D009842:Oligopeptides; D047011:Silk; D000520:alpha-Glucosidases