title: The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis.
abstract: Small non-coding RNAs regulate gene expression in a sequence-specific manner. In Drosophila, Dicer-2 (Dcr-2) functions in the biogenesis of endogenous small interfering RNAs (endo-siRNAs). We identified 21 distinct proteins that exhibited a ≥ 1.5-fold change as a consequence of loss of dcr-2 function. Most of these were metabolic genes implicated in stress resistance and aging. dcr-2 Mutants had reduced lifespan and were hypersensitive to oxidative, endoplasmic reticulum, starvation, and cold stresses. Furthermore, loss of dcr-2 function led to abnormal lipid and carbohydrate metabolism. Our results suggest roles for the endo-siRNA pathway in metabolic regulation and defense against stress and aging in Drosophila.
authors: College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors: Do-Hwan
authors: DH
authors: Lim
authors: Chun-Taek
authors: CT
authors: Oh
authors: Langho
authors: L
authors: Lee
authors: Jae-Sang
authors: JS
authors: Hong
authors: Su-Hyun
authors: SH
authors: Noh
authors: Seungwoo
authors: S
authors: Hwang
authors: Sungchan
authors: S
authors: Kim
authors: Sung-Jun
authors: SJ
authors: Han
authors: Young Sik
authors: YS
authors: Lee
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D004734:Energy Metabolism / Q000235:genetics*; D005260:Female; D006706:Homeostasis / Q000235:genetics*; D008017:Life Expectancy; D008297:Male; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D034741:RNA, Small Interfering; C478069:DCR-2 protein, Drosophila; C515033:Dcr-1 protein, Drosophila; D043244:Ribonuclease III; D020365:RNA Helicases