title: The involvement of the HO-1 pathway in the anti-inflammatory action of a sulfated polysaccharide isolated from the red seaweed Gracilaria birdiae.
abstract: OBJECTIVE
  The aim of this study was to investigate the involvement of the hemoxigenase-1 (HO-1) pathway in the anti-inflammatory action of a sulfated polysaccharide from the red seaweed Gracilaria birdiae (SP-Gb).
  
  
  METHODS
  SP-Gb (5, 10 and 20 mg/kg) was administered to Wistar rats in a peritonitis model using carrageenan or a paw edema model using carrageenan or dextran. To analyze the involvement of HO-1 in the anti-inflammatory activity of SP-Gb, the animals were pretreated subcutaneously with a specific HO-1 inhibitor (ZnPP IX). To evaluate the systemic effects, SP-Gb (10 mg/kg) was administered to mice intraperitoneally before waiting for 48 h or for 14 days.
  
  
  RESULTS
  SP-Gb (10 mg/kg) caused an anti-inflammatory effect that was evidenced by a decrease in leukocytes in the peritoneal cavity. SP-Gb also reduced the paw edema induced by carrageenan and inhibited the paw edema induced by dextran in the first half-hour. After being inhibited by ZnPP IX, the anti-inflammatory effect of SP-Gb on carrageenan-induced rat paw edema was not observed. SP-Gb did not cause mortality or significant changes in the biochemical, hematological and histopathological parameters.
  
  
  CONCLUSIONS
  SP-Gb may be used as a tool for further investigations into the inflammatory processes associated with the hemoxigenase-1 pathway.
authors: Department of Biochemistry, Molecular Biology, Federal University of Ceará, Campus do Pici, Bloco 907, Fortaleza, CE, 60455-760, Brazil.
authors: Edfranck
authors: E
authors: de Sousa Oliveira Vanderlei
authors: Ianna Wivianne Fernandes
authors: IW
authors: de Araújo
authors: Ana Luíza Gomes
authors: AL
authors: Quinderé
authors: Bruno Pedrosa
authors: BP
authors: Fontes
authors: Ygor Raphael Gomes
authors: YR
authors: Eloy
authors: José Ariévilo Gurgel
authors: JA
authors: Rodrigues
authors: Antonio Alfredo Rodrigues
authors: AA
authors: e Silva
authors: Hellíada Vasconcelos
authors: HV
authors: Chaves
authors: Roberta Jeane Bezerra
authors: RJ
authors: Jorge
authors: Dalgimar Beserra
authors: DB
authors: de Menezes
authors: Janaína Serra Azul Monteiro
authors: JS
authors: Evangelista
authors: Mirna Marques
authors: MM
authors: Bezerra
authors: Norma Maria Barros
authors: NM
authors: Benevides
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan; D003911:Dextrans; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D044724:Gracilaria / Q000737:chemistry*; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000276:immunology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology*; D009195:Peroxidase / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000893:Anti-Inflammatory Agents; D003911:Dextrans; D004791:Enzyme Inhibitors; D011134:Polysaccharides; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D002351:Carrageenan; D009195:Peroxidase; D051547:Heme Oxygenase-1