title: Preparation of a heat-shock protein 70-based vaccine from DC-tumor fusion cells.
abstract: We have developed an enhanced molecular chaperone-based vaccine through rapid isolation of heat-shock protein 70 peptide complexes (Hsp70.PC) after the fusion of tumor and dendritic cells (DCs) (Hsp70.PC-F). In this approach, the tumor antigens are introduced into the antigen-processing machinery of dendritic cells through the cell fusion process and, thus, we can obtain antigenic tumor peptides or their intermediates that have been processed by dendritic cells. Our results show that Hsp70.PC-F has increased immunogenicity compared to preparations from tumor cells alone and, therefore, constitutes an improved formulation of chaperone protein-based tumor vaccine.
authors: Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
authors: Desheng
authors: D
authors: Weng
authors: Stuart K
authors: SK
authors: Calderwood
authors: Jianlin
authors: J
authors: Gong
mesh_terms: D002459:Cell Fusion; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D018832:Molecular Chaperones; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D014614:Vaccines, Synthetic