title: Royal jelly modulates oxidative stress and apoptosis in liver and kidneys of rats treated with cisplatin.
abstract: Cisplatin (CDDP) is one of the most active cytotoxic agents in the treatment of cancer and has adverse side effects such as nephrotoxicity and hepatotoxicity. The present study was designed to determine the effects of royal jelly (RJ) against oxidative stress caused by CDDP injury of the kidneys and liver, by measuring tissue biochemical and antioxidant parameters and investigating apoptosis immunohistochemically. Twenty-four Sprague Dawley rats were divided into four groups, group C: control group received 0.9% saline; group CDDP: injected i.p. with cisplatin (CDDP, 7 mg kg(-1) body weight i.p., single dose); group RJ: treated for 15 consecutive days by gavage with RJ (300 mg/kg/day); group RJ + CDDP: treated by gavage with RJ 15 days following a single injection of CDDP. Malondialdehyde (MDA) and glutathione (GSH) levels, glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activities were determined in liver and kidney homogenates, and the liver and kidney were also histologically examined. RJ elicited a significant protective effect towards liver and kidney by decreasing the level of lipid peroxidation (MDA), elevating the level of GSH, and increasing the activities of GST, GSH-Px, and SOD. In the immunohistochemical examinations were observed significantly enhanced apoptotic cell numbers and degenerative changes by cisplatin, but these histological changes were lower in the liver and kidney tissues of RJ + CDDP group. Besides, treatment with RJ lead to an increase in antiapoptotic activity hepatocytes and tubular epithelium. In conclusion, RJ may be used in combination with cisplatin in chemotherapy to improve cisplatin-induced oxidative stress parameters and apoptotic activity.
authors: Department of Physiology, Faculty of Veterinary Medicine, University of Atatürk, Erzurum, Turkey. karadenizali@gmail.com
authors: Ali
authors: A
authors: Karadeniz
authors: Nejdet
authors: N
authors: Simsek
authors: Emre
authors: E
authors: Karakus
authors: Serap
authors: S
authors: Yildirim
authors: Adem
authors: A
authors: Kara
authors: Ismail
authors: I
authors: Can
authors: Fikrullah
authors: F
authors: Kisa
authors: Habib
authors: H
authors: Emre
authors: Mehmet
authors: M
authors: Turkeli
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D000099219:Royal Jelly
chemical_list: D002945:Cisplatin; D005227:Fatty Acids; D005978:Glutathione; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D008315:Malondialdehyde; D000099219:Royal Jelly