title: Involvement of angiotensin II type 1 receptors in interleukin-1β-induced interleukin-6 production in human gingival fibroblasts.
abstract: The renin-angiotensin system is thought to be involved in inflammatory processes such as periodontitis. However, its precise role is still unclear. Therefore, in the present study the expression of the angiotensin II type 1 receptor (AT1R) was investigated in inflamed human gingival tissue, and the possible involvement of the AT1R in interleukin-1β (IL-1β)-induced interleukin-6 (IL-6) production by cultured human gingival fibroblasts (HGFs) was also studied. Immunohistochemical staining revealed that inflammatory cells and fibroblast-like cells were positive for the AT1R. However, in healthy gingival tissue, AT1R staining was very weak. The levels of AT1R mRNA and AT1R protein increased in HGFs after stimulation with IL-1β. The levels of IL-1β-induced IL6 mRNA and IL-6 protein were significantly reduced in AT1R gene-silenced HGFs compared with control HGFs. The data suggest that the AT1R may be involved in the regulation of gingival inflammation by modulating IL-1β-induced IL-6 production in HGFs.
authors: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Sakuragaoka, Japan. toshi-n@dent.kagoshima-u.ac.jp
authors: Toshiaki
authors: T
authors: Nakamura
authors: Kozue
authors: K
authors: Hasegawa-Nakamura
authors: Kenji
authors: K
authors: Sakoda
authors: Takashi
authors: T
authors: Matsuyama
authors: Kazuyuki
authors: K
authors: Noguchi
mesh_terms: D000808:Angiotensinogen / Q000032:analysis; D002402:Cathepsin D / Q000032:analysis; D002478:Cells, Cultured; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D020868:Gene Silencing; D005881:Gingiva / Q000187:drug effects / Q000378:metabolism*; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology*; D015850:Interleukin-6 / Q000032:analysis* / Q000096:biosynthesis; D007703:Peptidyl-Dipeptidase A / Q000032:analysis; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D044140:Receptor, Angiotensin, Type 1 / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D012083:Renin / Q000032:analysis; D012084:Renin-Angiotensin System / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C544065:AGTR1 protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D034741:RNA, Small Interfering; D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A; D012083:Renin; C533922:CTSD protein, human; D002402:Cathepsin D