title: Staudinger ligation as a method for bioconjugation.
abstract: In 1919 the German chemist Hermann Staudinger was the first to describe the reaction between an azide and a phosphine. It was not until recently, however, that Bertozzi and co-workers recognized the potential of this reaction as a method for bioconjugation and transformed it into the so-called Staudinger ligation. The bio-orthogonal character of both the azide and the phosphine functions has resulted in the Staudinger ligation finding numerous applications in various complex biological systems. For example, the Staudinger ligation has been utilized to label glycans, lipids, DNA, and proteins. Moreover, the Staudinger ligation has been used as a synthetic method to construct glycopeptides, microarrays, and functional biopolymers. In the emerging field of bio-orthogonal ligation strategies, the Staudinger ligation has set a high standard to which most of the new techniques are often compared. This Review summarizes recent developments and new applications of the Staudinger ligation.
authors: Department of Bioorganic Chemistry, Radboud University Nijmegen, Heyendaalseweg 135, NL-6525 AJ Nijmegen, The Netherlands.
authors: Sander S
authors: SS
authors: van Berkel
authors: Mark B
authors: MB
authors: van Eldijk
authors: Jan C M
authors: JC
authors: van Hest
mesh_terms: D001386:Azides / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
chemical_list: D001386:Azides; D008055:Lipids; D010720:Phosphines; D011134:Polysaccharides; D011506:Proteins; D004247:DNA; C044646:phosphine