title: Cofactor-free detection of phosphatidylserine with cyclic peptides mimicking lactadherin.
abstract: Cyclic peptides (cLacs) are designed to mimic the natural phosphatidylserine (PS) binding protein lactadherin. Unlike annexin V or its small molecule mimics, the cLac peptides selectively target PS-presenting membranes with no need for metal cofactors. We further show that a fluorophore-labeled cLac effectively stains early apoptotic cells. The small size and facile conjugation with a variety of imaging tracers make the cLac design promising for imaging cell death in vitro as well as in living organisms.
authors: Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, Massachusetts 02467, United States.
authors: Hong
authors: H
authors: Zheng
authors: Fang
authors: F
authors: Wang
authors: Qin
authors: Q
authors: Wang
authors: Jianmin
authors: J
authors: Gao
mesh_terms: D015195:Drug Design; D006801:Humans; D019169:Jurkat Cells; D008894:Milk Proteins / Q000737:chemistry*; D008958:Models, Molecular; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000378:metabolism*; D057786:Peptidomimetics / Q000737:chemistry* / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D008894:Milk Proteins; D010456:Peptides, Cyclic; D057786:Peptidomimetics; D010718:Phosphatidylserines