title: MHC Class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains.
abstract: Eosinophils function in murine allergic airways inflammation as professional antigen-presenting cells (APCs). In murine professional APC cell types, optimal functioning of MHC Class II depends on its lateral association in plasma membranes and colocalization with the tetraspanin CD9 into detergent-resistant membrane microdomains (DRMs). With human eosinophils, we evaluated the localization of MHC Class II (HLA-DR) to DRMs and the functional significance of such localization. In granulocyte-macrophage colony-stimulating factor-stimulated human eosinophils, antibody cross-linked HLA-DR colocalized by immunofluorescence microscopy focally on plasma membranes with CD9 and the DRM marker ganglioside GM1. In addition, HLA-DR coimmunoprecipitates with CD9 after chemical cross-linking of CD9. HLA-DR and CD9 were localized by Western blotting in eosinophil DRM subcellular fractions. DRM disruption with the cholesterol-depleting agent methyl-β-cyclodextrin decreased eosinophil surface expression of HLA-DR and CD9. We show that CD9 is abundant on the surface of eosinophils, presenting the first electron microscopy data of the ultrastructural immunolocalization of CD9 in human eosinophils. Disruption of HLA-DR-containing DRMs decreased the ability of superantigen-loaded human eosinophils to stimulate CD4(+) T-cell activation (CD69 expression), proliferation, and cytokine production. Our results, which demonstrate that eosinophil MHC Class II localizes to DRMs in association with CD9 in a functionally significant manner, represent a novel insight into the organization of the antigen presentation complex of human eosinophils.
authors: Department of Medicine, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA.
authors: Praveen
authors: P
authors: Akuthota
authors: Rossana C N
authors: RC
authors: Melo
authors: Lisa A
authors: LA
authors: Spencer
authors: Peter F
authors: PF
authors: Weller
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D003902:Detergents; D004804:Eosinophils / Q000378:metabolism*; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D008567:Membranes, Artificial; D060245:Tetraspanin 29 / Q000378:metabolism*
chemical_list: C554869:CD9 protein, human; D003902:Detergents; D000949:Histocompatibility Antigens Class II; D008567:Membranes, Artificial; D060245:Tetraspanin 29