title: A rapid and cost-effective method for sequencing pooled cDNA clones by using a combination of transposon insertion and Gateway technology.
abstract: Large-scale cDNA-sequencing projects require an efficient strategy for mass sequencing. Here we describe a method for sequencing pooled cDNA clones using a combination of transposon insertion and Gateway technology. Our method reduces the number of shotgun clones that are unsuitable for reconstruction of cDNA sequences, and has the advantage of reducing the total costs of the sequencing project.
authors: Animal Genome Research Program, Animal Research Division, Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Japan.
authors: Takeya
authors: T
authors: Morozumi
authors: Daisuke
authors: D
authors: Toki
authors: Tomoko
authors: T
authors: Eguchi-Ogawa
authors: Hirohide
authors: H
authors: Uenishi
mesh_terms: D000818:Animals; D003362:Cost-Benefit Analysis; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000191:economics / Q000379:methods*; D016254:Mutagenesis, Insertional / Q000379:methods*; D013552:Swine / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary