title: Characterization of parvoviruses from domestic cats in Brazil.
abstract: To characterize Feline parvovirus (FPV) circulating in domestic cats in Brazil, 51 fecal samples from unvaccinated domestic cats were collected during 2004-2005. Six parvoviruses were characterized by hemagglutination (HA) assay at different pH values and temperatures and by polymerase chain reaction (PCR) using different pairs of primers. However, data obtained from HA and PCR did not allow the discrimination between FPV and Canine parvovirus (CPV). Two regions of the VP2 capsid gene (1,171-bp fragment) involved in controlling canine and feline host range were sequenced; 9 synonymous and 10 non-synonymous nucleotide substitutions were detected. All samples were confirmed as FPV by nucleotide sequencing, but 3 feline samples had amino acid changes at residues 93, 375, and 426, which are present in canine strains. The phylogenetic tree built based on nucleotide sequences showed that Brazilian feline samples form a cluster distinct from other parvoviruses deposited in GenBank. Taken together, the findings reinforce the importance of monitoring the continuous evolution of CPV and FPV in the feline population in Brazil.
authors: Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Prof. Ernani Pires de Melo, 101 24210-130 Niterói, Rio de Janeiro, Brazil. ritacubel@id.uff.br
authors: Rita de Cássia Nasser Cubel
authors: Rde C
authors: Garcia
authors: Tatiana Xavier
authors: TX
authors: de Castro
authors: Suzana Carvalho
authors: SC
authors: de Miranda
authors: Gláucio
authors: G
authors: Lopes
authors: Marcelo
authors: M
authors: de Lima
authors: Norma Volmer
authors: NV
authors: Labarthe
authors: José Paulo Gagliardi
authors: JP
authors: Leite
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D036022:Capsid Proteins / Q000235:genetics; D002371:Cat Diseases / Q000453:epidemiology / Q000821:virology*; D002415:Cats; D005243:Feces / Q000821:virology; D010321:Parvoviridae / Q000145:classification* / Q000302:isolation & purification*; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D013045:Species Specificity
chemical_list: D036022:Capsid Proteins