title: Generation of spatial patterns through cell polarity switching.
abstract: The mechanisms that generate dynamic spatial patterns within proliferating tissues are poorly understood, largely because of difficulties in unravelling interactions between cell specification, polarity, asymmetric division, rearrangements, and growth. We address this problem for stomatal spacing in plants, which offer the simplifying advantage that cells do not rearrange. By tracking lineages and gene activities over extended periods, we show that limited stem cell behavior of stomatal precursors depends on maintenance of the SPEECHLESS (SPCH) transcription factor in single daughter cells. Modeling shows how this property can lead to observed stereotypical stomata lineages through a postmitotic polarity-switching mechanism. The model predicts the location of a polarity determinant BASL over multiple divisions, which we validate experimentally. Our results highlight the dynamic two-way interactions between stem cells and their neighborhood during developmental patterning.
authors: John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
authors: Sarah
authors: S
authors: Robinson
authors: Pierre
authors: P
authors: Barbier de Reuille
authors: Jordi
authors: J
authors: Chan
authors: Dominique
authors: D
authors: Bergmann
authors: Przemyslaw
authors: P
authors: Prusinkiewicz
authors: Enrico
authors: E
authors: Coen
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D016764:Cell Polarity; D048429:Cell Size; D018519:Meristem / Q000166:cytology*; D018613:Microscopy, Confocal; D008954:Models, Biological; D019441:Plant Epidermis / Q000166:cytology; D018515:Plant Leaves / Q000166:cytology; D054046:Plant Stomata / Q000166:cytology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D018797:Cell Cycle Proteins; D011993:Recombinant Fusion Proteins; C542095:BASL protein, Arabidopsis; C517480:SPEECHLESS protein, Arabidopsis