title: Mediator subunit 12 is required for neutrophil development in zebrafish.
abstract: Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.
authors: Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors: Maria-Cristina
authors: MC
authors: Keightley
authors: Judith E
authors: JE
authors: Layton
authors: John W
authors: JW
authors: Hayman
authors: Joan K
authors: JK
authors: Heath
authors: Graham J
authors: GJ
authors: Lieschke
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D004920:Erythropoiesis / Q000235:genetics / Q000502:physiology; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism*; D020125:Mutation, Missense / Q000235:genetics; D038042:Myelopoiesis / Q000235:genetics / Q000502:physiology; D009504:Neutrophils / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C513481:Med12 protein, zebrafish; D056892:Mediator Complex; D029961:Zebrafish Proteins