title: Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage.
abstract: A multianalyte method was developed to identify and quantitate 26 mycotoxins simultaneously in maize silage by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The extraction and cleanup procedure consists of two extraction steps followed by purification on a Waters Oasis HLB column. The method developed was validated with the requirements of Commission Decision 2002/657/EC taken into account. The limit of detection and quantitation ranges were 5-348 and 11-695 ng/g, respectively. Apparent recovery varied between 61 and 116%, whereas repeatability and reproducibility were within the ranges of 3-45 and 5-49%, respectively. The method developed was successfully applied for maize silage samples taken at the cutting surface and 1 m behind that surface. Mainly Fusarium toxins (beauvericin, deoxynivalenol, enniatins, fumonisins, fusaric acid, and zearalenone) were detected, but postharvest toxins such as mycophenolic acid and roquefortine C were identified as well.
authors: Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, 9090 Melle, Belgium. els.vanpamel@ilvo.vlaanderen.be
authors: Els
authors: E
authors: Van Pamel
authors: Annemieke
authors: A
authors: Verbeken
authors: Geertrui
authors: G
authors: Vlaemynck
authors: Johan
authors: J
authors: De Boever
authors: Els
authors: E
authors: Daeseleire
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D009183:Mycotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012820:Silage / Q000032:analysis* / Q000382:microbiology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology
chemical_list: D009183:Mycotoxins