title: Mycobacterium tuberculosis Beijing genotype induces differential cytokine production by peripheral blood mononuclear cells of healthy BCG vaccinated individuals.
abstract: Members of the Mycobacterium tuberculosis (Mtb) Beijing genotype are a major concern due to their high prevalence in tuberculosis patients and their high rate of multi-drug resistance. Although it has been shown that Beijing modifies macrophage behavior, little is known about how this genotype could affect the cellular immune response. In order to address this issue, peripheral blood mononuclear cells (PBMC) from healthy BCG vaccinated individuals were stimulated with protein extracts from three Mycobacterium tuberculosis genotypes: Canetti, H37Rv and Beijing evaluating T cell proliferation and cytokine production. In this system both CD4+ and CD8+ proliferated in a similar manner independently of the Mtb genotype used for stimulation. Regarding cytokines, all strains induced similar levels of IFN-γ, but were unable to induce IL-4 and TGF-β. Contrasting, Canetti strain induced lower production of IL-10, TNF-α and IL-12 compared to H37Rv and Beijing. Interestingly, PBMC stimulated with the Beijing strain produced the highest levels of IL-12 and IL-10 than those stimulated with other strains. This differential cytokine expression could affect the pathogenesis induced by Beijing strain through the modulation of inflammatory process in the host, but the precise mechanisms by which this cytokine environment affects the Beijing strain pathogenesis needs further characterization.
authors: Department of Immunology, National School of Biological Sciences, ENCB-IPN, Mexico.
authors: Araceli
authors: A
authors: Rivera-Ordaz
authors: Job
authors: J
authors: Gonzaga-Bernachi
authors: Jeanet
authors: J
authors: Serafín-López
authors: Rogelio
authors: R
authors: Hernández-Pando
authors: Dick
authors: D
authors: Van Soolingen
authors: Sergio
authors: S
authors: Estrada-Parra
authors: Iris
authors: I
authors: Estrada-García
authors: Rommel
authors: R
authors: Chacón-Salinas
mesh_terms: D000328:Adult; D001500:BCG Vaccine / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D049109:Cell Proliferation; D002478:Cells, Cultured; D002681:China; D016207:Cytokines / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000382:microbiology; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D014397:Tuberculosis, Pulmonary / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination; D055815:Young Adult
chemical_list: D001500:BCG Vaccine; D016207:Cytokines; D004269:DNA, Bacterial