title: Impact of substrates for probe immobilization.
abstract: Protein chips are becoming a key technology in proteomic research and medical diagnostics. Surface chemistry for immobilization of proteins forms the basis for assay design and determines the properties of protein microarrays. Optimal substrates provide a homogeneous environment for probes, preventing loss of biological activity and unspecific adsorption. Numerous immobilization approaches, based on covalent binding, affinity, or adsorption, have been proposed thus far, and these represent the toolbox for choosing optimized strategies for each individual application.
authors: Health & Environment Department, Biosensor Technologies, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria. Ursula.Sauer@ait.ac.at
authors: Ursula
authors: U
authors: Sauer
mesh_terms: D000327:Adsorption; D020100:Hydrogels; D055767:Immobilized Proteins / Q000737:chemistry*; D008958:Models, Molecular; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D040901:Proteomics / Q000379:methods*; D013499:Surface Properties
chemical_list: D020100:Hydrogels; D055767:Immobilized Proteins