title: Role of inhibitor of apoptosis protein Livin in radiation resistance in nonsmall cell lung cancer.
abstract: OBJECTIVE
  The objective of the present study was to explore the role of the inhibitor of apoptosis protein (IAP) Livin in radioresistance in nonsmall cell lung cancer (NSCLC).
  
  
  METHODS
  Lung adenocarcinoma cell lines A549 and SPC-A1 were used for this study. Using the technique of molecular cloning and gene transfection, two Livin isoforms, Livinα and β, respectively, were expressed in A549 cells with the purpose of exploring the role of Livin in radiation resistance of A549 cells. Moreover, a Livin-specific gene-silencing system was developed using SPC-A1 cell line with the purpose of increasing radiosensitivity of SPC-A1 cells.
  
  
  RESULTS
  A549 cells were induced by radiation to express Livin isoforms, Livinα and β. A549 cells expressed Livin isoforms stably after gene transfection and the transfected cells demonstrated characteristics of antiradiation. However, Livin gene-silenced SPC-A1 cells exhibited remarkably enhanced radiation sensitivity.
  
  
  CONCLUSIONS
  The IAP Livin is an important molecule in antiradiotherapy of NSCLC. Livin-specific gene silencing is likely to be an effective means to enhance radiation sensitivity of lung cancer.
authors: Cancer Institute of People's Liberation Army, Xinqiao Hospital, Third Military Medical University, 30 Gootanyan Main Street, Chongqing, P.R. China.sunjg09@yahoo.com.cn
authors: Jian-Guo
authors: JG
authors: Sun
authors: Rong-Xia
authors: RX
authors: Liao
authors: Shao-Xiang
authors: SX
authors: Zhang
authors: Yu-Zhong
authors: YZ
authors: Duan
authors: Wen-Lei
authors: WL
authors: Zhuo
authors: Xin-Xin
authors: XX
authors: Wang
authors: Zhi-Xin
authors: ZX
authors: Wang
authors: De-Zhi
authors: DZ
authors: Li
authors: Zheng-Tang
authors: ZT
authors: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D045744:Cell Line, Tumor; D020868:Gene Silencing; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics; D011836:Radiation Tolerance; D014162:Transfection
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C419866:BIRC7 protein, human; D051034:Inhibitor of Apoptosis Proteins; D009363:Neoplasm Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D034741:RNA, Small Interfering