title: A rapid PCR-based approach for molecular identification of filamentous fungi.
abstract: In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.
authors: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
authors: Yuanyuan
authors: Y
authors: Chen
authors: Bernard A
authors: BA
authors: Prior
authors: Guiyang
authors: G
authors: Shi
authors: Zhengxiang
authors: Z
authors: Wang
mesh_terms: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer