title: Extracellular secretion of anticoagulant peptide hirudin in Lactococcus lactis using SP310mut2 signal peptide.
abstract: Hirudin can be used as an oral anticoagulant and antithrombotic agent. The hirudin variant III gene, derived from the medicinal leech, Hirudo medicinalis, was fused to SP310mut2 signal sequence and expressed by a nisin-controlled gene expression system in Lactococcus lactis which was then grown in a 7 l fermenter. After induction with 8 ng nisin ml(-1), the product was secreted into the culture medium and accumulated up to ~2.7 mg l(-1). MALDI-TOF/MS and anticoagulant activity analyses on the purified product confirmed its authenticity. This is the first demonstration that hirudin can be extracellularly secreted and correctly processed in L. lactis.
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, 211# mailbox, 24 Tongjia Street, Nanjing, 210009, People's Republic of China. lvjing0543311@126.com
authors: Jing
authors: J
authors: Lv
authors: Cuicui
authors: C
authors: Huang
authors: Xuerui
authors: X
authors: Zhang
authors: Shuhua
authors: S
authors: Tan
mesh_terms: D000818:Animals; D006629:Hirudins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047709:Hirudo medicinalis / Q000235:genetics; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006629:Hirudins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins