title: Detection of invasive infection caused by Fusarium solani and non-Fusarium solani species using a duplex quantitative PCR-based assay in a murine model of fusariosis.
abstract: A duplex Real Time PCR (RT-PCR) assay for detecting DNA of members of the genus Fusarium has been developed and validated by using two mouse models of invasive infection. The duplex RT-PCR technique employed two specific molecular beacon probes targeting a highly conserved region of the fungal rDNA gene. This technique showed a detection limit of 10 fg DNA per μl of sample and a specificity of 100%. The sensitivity in a total of 48 samples from a murine model of Fusarium solani infection was 93.9% for lung tissues and 86.7% for serum samples. In comparison, the sensitivity in a total of 45 samples of a F. oxysporum murine model infection was 87% for lung tissues and 42.8% for serum samples. This molecular technique could be a reliable method for the quantification and the evaluation of the disease in animal models and for the clinical diagnosis of fusariosis.
authors: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
authors: Leticia
authors: L
authors: Bernal-Martínez
authors: Maria J
authors: MJ
authors: Buitrago
authors: Maria V
authors: MV
authors: Castelli
authors: Juan L
authors: JL
authors: Rodríguez-Tudela
authors: Manuel
authors: M
authors: Cuenca-Estrella
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics; D004195:Disease Models, Animal; D060585:Fusariosis / Q000175:diagnosis*; D005670:Fusarium / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009172:Mycology / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D015345:Oligonucleotide Probes