title: Molecular divergence in the timeless and cpr genes among three sympatric cryptic species of the Anopheles triannulatus complex.
abstract: Anopheles triannulatus s.l. is a malaria vector with a wide geographic distribution, ranging from Argentina-Nicaragua and Trinidad. Here we analysed sequences of two genes, timeless and cpr, to assess the genetic variability and divergence among three sympatric cryptic species of this complex from Salobra, central-western Brazil. The timeless gene sequences did not conclusively differentiate Anopheles halophylus and An. triannulatus species "C". However, a partial separation has been observed between these species and An. triannulatus s.s. Importantly, the analysis of the cpr gene sequences revealed fixed differences, no shared polymorphisms and considerable genetic differentiation among the three species of the An. triannulatus complex. The results confirm that An. triannulatus s.s., An. halophylus and An. triannulatus species C are distinct taxa, with the latter two likely representing a more recent speciation event.
authors: Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil. tsilva@ioc.fiocruz.br
authors: Teresa Fernandes
authors: TF
authors: Silva-do-Nascimento
authors: Luisa Damazio Rona
authors: LD
authors: Pitaluga
authors: Alexandre Afranio
authors: AA
authors: Peixoto
authors: Ricardo
authors: R
authors: Lourenço-de-Oliveira
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification / Q000235:genetics*; D005260:Female; D017344:Genes, Insect / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D007303:Insect Vectors / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013045:Species Specificity