title: Quantification of pork, chicken and beef by using a novel reference molecule.
abstract: A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R² values of the standard curves (10³-10⁷ copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.
authors: Biochemical Production and Development Center, Nagahama Branch, Oriental Yeast Co., Ltd.
authors: Yumiko
authors: Y
authors: Sakai
authors: Satoshi
authors: S
authors: Kotoura
authors: Takeo
authors: T
authors: Yano
authors: Takashi
authors: T
authors: Kurihara
authors: Kouji
authors: K
authors: Uchida
authors: Kiyotaka
authors: K
authors: Miake
authors: Hiroshi
authors: H
authors: Akiyama
authors: Soichi
authors: S
authors: Tanabe
mesh_terms: D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D002417:Cattle; D002645:Chickens; D045303:Cytochromes b / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics*; D015342:DNA Probes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli; D018628:Gene Dosage; D005813:Genes, Synthetic; D006736:Horses; D008461:Meat Products / Q000032:analysis*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012756:Sheep; D013552:Swine
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D045303:Cytochromes b