title: Notch signaling is necessary for adult, but not fetal, development of RORγt(+) innate lymphoid cells.
abstract: The transcription factor RORγt is required for the development of several innate lymphoid populations, such as lymphoid tissue-inducer cells (LTi cells) and cells that secrete interleukin 17 (IL-17) or IL-22. The progenitor cells as well as the developmental stages that lead to the emergence of RORγt(+) innate lymphoid cells (ILCs) remain undefined. Here we identify the chemokine receptor CXCR6 as an additional marker of the development of ILCs and show that common lymphoid progenitors lost B cell and T cell potential as they successively acquired expression of the integrin α(4)β(7) and CXCR6. Whereas fetal RORγt(+) cells matured in the fetal liver environment, adult bone marrow-derived RORγt(+) ILCs matured outside the bone marrow, in a Notch2-dependent manner. Therefore, fetal and adult environments influence the differentiation of RORγt(+) cells differently.
authors: Institut Pasteur, Unité de Lymphopoièse, Paris, France.
authors: Cécilie
authors: C
authors: Possot
authors: Sandrine
authors: S
authors: Schmutz
authors: Sylvestre
authors: S
authors: Chea
authors: Laurent
authors: L
authors: Boucontet
authors: Anne
authors: A
authors: Louise
authors: Ana
authors: A
authors: Cumano
authors: Rachel
authors: R
authors: Golub
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000502:physiology; D005333:Fetus / Q000276:immunology*; D007113:Immunity, Innate; D016023:Integrins / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000502:physiology*; D051883:Receptor, Notch2 / Q000502:physiology*; D054387:Receptors, CXCR / Q000502:physiology; D000075744:Receptors, CXCR6; D015398:Signal Transduction
chemical_list: C482906:Cxcr6 protein, mouse; D004268:DNA-Binding Proteins; D016023:Integrins; C497765:Notch2 protein, mouse; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C499308:Rag2 protein, mouse; D051883:Receptor, Notch2; D054387:Receptors, CXCR; D000075744:Receptors, CXCR6; C071777:integrin alpha4beta7