title: Effect of deoxycholate conjugation on stability of pDNA/polyamidoamine-diethylentriamine (PAM-DET) polyplex against ionic strength.
abstract: Polyplexes formed from cationic polymer/pDNA have been known to be vulnerable to external ionic strength. To improve polyplex stability against ionic strength, we attempted the chemical conjugation of the hydrophobic deoxycholate (DC) moiety to the polyamidoamine-diethylenetriamine (PAM-DET) dendrimer. Dynamic light scattering studies showed that the tolerance of the resulting PAM-DET-DC against ionic strength is higher than that of PAM-DET. In addition, we confirmed that the stability of polyplex has a strong relationship with the degree of conjugation of the DC moiety to the PAM-DET dendrimer and the charge ratio of PAM-DET-DC. Furthermore, the transfection efficiency of the PAM-DET-DC polyplex is higher than that of PAM-DET but its cytotoxicity remains the same. Therefore, the chemical conjugation of DC is a safe and effective method for increasing the stability of supramolecules formed from electrostatic interaction.
authors: Department of Chemistry, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors: Yunseong
authors: Y
authors: Jeong
authors: Geun-Woo
authors: GW
authors: Jin
authors: Eunjung
authors: E
authors: Choi
authors: Ji Hyuk
authors: JH
authors: Jung
authors: Jong-Sang
authors: JS
authors: Park
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000235:genetics; D003840:Deoxycholic Acid / Q000737:chemistry* / Q000494:pharmacology; D004355:Drug Stability; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D049408:Luminescent Agents / Q000378:metabolism; D009994:Osmolar Concentration; D010957:Plasmids / Q000737:chemistry* / Q000494:pharmacology; D011073:Polyamines / Q000737:chemistry* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
chemical_list: D049408:Luminescent Agents; C531249:Poly(amidoamine); D011073:Polyamines; D003840:Deoxycholic Acid; C005391:diethylenetriamine; D004247:DNA; D008156:Luciferases