title: Physiological and biochemical responses of Microcystis aeruginosa to phosphite.
abstract: Phosphorus (P) is a key biological element and limiting nutrient in aquatic environments. Phosphate (+5) is traditionally associated with the P nutrient supply. However, phosphite (+3) has recently generated a great deal of interest, because of the possibility that it is a P source based on recognition of its vital role in the original life of the early earth. This study investigated whether phosphite can be an alternative P source for Microcystis aeruginosa PCC 7806, one of the predominant bloom species in freshwater systems. The results indicated that M. aeruginosa could not utilize phosphite as a sole P-nutrient directly for cell growth at any concentration, but that phosphite could boost cell numbers and chlorophyll a (Chl-a) content as long as phosphate was provided simultaneously. Specifically, Chl-a production increased sharply when 5.44 mg PL(-1) phosphite was added to 0.54 mg PL(-1) phosphate medium. Analysis of the maximum yield of PSII indicated that phosphite may stimulate the photosynthesis process of cells in phosphate-phosphite medium. In addition, phosphite failed to support cell growth, even though it more readily permeated the cells in P-deficient medium than in P-sufficient medium. Alkaline phosphatase activity (APA) analysis indicated that, unlike organic P, phosphite inhibits the response of cells to deficient P status, especially under P-deprived conditions.
authors: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, PR China.
authors: Juan
authors: J
authors: Zhang
authors: Jinju
authors: J
authors: Geng
authors: Hongqiang
authors: H
authors: Ren
authors: Jun
authors: J
authors: Luo
authors: Aiqian
authors: A
authors: Zhang
authors: Xiaorong
authors: X
authors: Wang
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D005618:Fresh Water / Q000737:chemistry / Q000382:microbiology; D046931:Microcystis / Q000254:growth & development* / Q000378:metabolism*; D017905:Phosphites / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010788:Photosynthesis
chemical_list: D017905:Phosphites; D002734:Chlorophyll; D010758:Phosphorus; D000077194:Chlorophyll A