title: Zebrafish anti-apoptotic gene Bcl-xL can prevent aquatic birnavirus-induced cell death in fish cells without affecting expression of viral proteins.
abstract: The aquatic birnavirus induces mitochondria-mediated cell death in fish; however, the molecular mechanism remains unknown. In the present study, we demonstrated that aquatic birnavirus-induced mitochondria-mediated cell death is regulated by the anti-apoptotic Bcl-2 family member, zfBcl-xL, which is anti-apoptotic and enhances host cell viability. First, CHSE-214 cells carrying EGFP-zfBcl-xL fused genes were selected, established in culture, and used to examine the involvement of zfBcl-xL in host cell protection from the effects of viral infection. EGFP-zfBcl-xL was found to prevent infectious pancreatic necrosis virus (IPNV)-induced phosphatidylserine exposure up to 40% at 12 h and 24 h post-infection (p.i.), block IPNV-induced loss of mitochondrial membrane potential (ΔΨm), and enhance host viability at the middle and late replication stages. In addition, zfBcl-xL overexpression prevented IPNV-induced caspase-9 activation up to 25% and 85% at the middle (12 h p.i.) and late (24 h p.i.) replication stages without affecting expression of viral proteins such as VP3 (as a viral death protein) protein. In the present study, we demonstrated that aquatic birnavirus-induced cell death is prevented by the anti-apoptotic Bcl-2 family member, zfBcl-xL, which enhances host cell viability through blockage of mitochondrial disruption and caspase-9 activation.
authors: Laboratory of Molecular Virology and Biotechnology, Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
authors: Hui-Ling
authors: HL
authors: Huang
authors: Yen-Ting
authors: YT
authors: Liu
authors: Ming-Chyuan
authors: MC
authors: Chen
authors: Jen-Leih
authors: JL
authors: Wu
authors: Jiann-Ruey
authors: JR
authors: Hong
mesh_terms: D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D015153:Blotting, Western; D053453:Caspase 9 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008856:Microscopy, Fluorescence; D012474:Salmon; D015027:Zebrafish / Q000235:genetics*; D051020:bcl-X Protein / Q000235:genetics / Q000494:pharmacology*
chemical_list: D051020:bcl-X Protein; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D053453:Caspase 9; D020782:Matrix Metalloproteinases