title: Prothrombin complex concentrate reduces blood loss and enhances thrombin generation in a pig model with blunt liver injury under severe hypothermia.
abstract: Although prothrombin complex concentrate (PCC) is increasingly used for the treatment of trauma-induced coagulopathy, few studies have investigated the impact and safety of PCC for this indication. The present study was performed to assess PCC for treatment of coagulopathy after blunt liver injury under severe hypothermia. Coagulopathy in 14 anaesthetised pigs was induced by haemodilution. Subsequently, standardised blunt liver injury was induced under severe hypothermia (32.8-33.2°C). Animals were randomised to receive either PCC (35 IU kg⁻¹) or saline (control). Coagulation was assessed over the following 2 hours by thromboelastometry and thrombin generation. Internal organs were examined to determine presence of emboli. The administration of PCC showed a significant reduction in blood loss (p=0.002 vs. controls) and a significant increase in the rate of survival (p=0.022 vs. controls). Plasma thrombin generation in the PCC group increased considerably above baseline levels, with significant increases in peak thrombin levels and endogenous thrombin potential versus controls throughout the follow-up period. In addition, PT decreased significantly in the PCC group versus the control group. However, only slight improvements in thromboelastometry variables were observed. Histology showed an equal degree of liver injury in both groups, and no thromboembolism. In severely hypothermic pigs, the application of PCC corrected trauma-induced coagulopathy and reduced blood loss. Thus, the infusion of PCC might be a reasonable approach to reduce the need for blood cell transfusion in trauma. Furthermore, the impact and safety of PCC application can be monitored through thrombin generation and thromboelastometry under hypothermia.
authors: Department of Anaesthesiology, RWTH Aachen University Hospital, Aachen, Germany.
authors: Markus
authors: M
authors: Honickel
authors: Annette
authors: A
authors: Rieg
authors: Rolf
authors: R
authors: Rossaint
authors: Till
authors: T
authors: Braunschweig
authors: Henri M H
authors: HM
authors: Spronk
authors: Hugo
authors: H
authors: ten Cate
authors: Rene
authors: R
authors: van Oerle
authors: Rene
authors: R
authors: Tolba
authors: Oliver
authors: O
authors: Grottke
mesh_terms: D000818:Animals; D001778:Blood Coagulation Disorders / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D001779:Blood Coagulation Factors / Q000008:administration & dosage* / Q000009:adverse effects; D004195:Disease Models, Animal; D006470:Hemorrhage; D006801:Humans; D007035:Hypothermia; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D013552:Swine; D013916:Thrombelastography; D013917:Thrombin / Q000378:metabolism; D014949:Wounds, Nonpenetrating / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology
chemical_list: D001779:Blood Coagulation Factors; C025667:prothrombin complex concentrates; D013917:Thrombin