title: Antioxidant, anti-lipoxygenase and cytotoxic activity of Leptadenia pyrotechnica (Forssk.) decne polyphenolic constituents.
abstract: Leptadenia pyrotechnica Forssk is a traditional medicinal herb used for treatment of inflammatory diseases and cancer. In this research, the aqueous ethanolic crude extract of Leptadenia pyrotechnica aerial parts, along with its ethyl acetate, n-butanol and water partitioning fractions were evaluated for their antioxidant capacity, polyphenolic content, anti-inflammatory and anti-cancer properties. The total antioxidant capacity was estimated by the FRAP, DPPH, ABTS and β-carotene bleaching assays.The ethyl acetate fraction exhibited the highest polyphenolic content (252.27 mg gallic acid/g) and the best antioxidant activity (1.2, 0.57, 0.45 mmol ascorbic acid equivalent/g in the FRAP, ABTS and DPPH assays, respectively). Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase (LOX) inhibitory activity (IC₅₀ = 1.41 µg/mL). Moreover, the ethyl acetate fraction also showed the strongest cytotoxic effect (IC₅₀ = 43.16 µg/mL) against MCF-7 human breast cancer cell line. These results suggest that this plant may be considered an interesting source of compounds with antioxidant, anti-inflammatory and anti-cancer properties for therapeutic, nutraceutical and functional food applications.
authors: Department of Chemistry, Faculty of Science U.A.E. University, Al-Ain, P.O. Box 17551, UAE. mohammad.khasawneh@uaeu.ac.ae
authors: Mohammad A
authors: MA
authors: Khasawneh
authors: Hanan M
authors: HM
authors: Elwy
authors: Alaaeldin A
authors: AA
authors: Hamza
authors: Nael M
authors: NM
authors: Fawzi
authors: Ahmed H
authors: AH
authors: Hassan
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry*; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D057075:Enzyme Assays; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D010851:Picrates / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D016859:Lipoxygenase Inhibitors; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl