title: The impact of aridification and vegetation type on changes in the community structure of methane-cycling microorganisms in Japanese wetland soils.
abstract: Over the years, the wetlands covered by Sphagnum in Bibai, Japan have been turning into areas of aridity, resulting in an invasion of Sasa into the bogs. Yet little is known about the methane-cycling microorganisms in such environments. In this study, the methanotrophic, methanogenic, and archaeal community structures within these two types of wetland vegetation were studied by phylogenetic analysis targeting particulate methane monooxygenase (pmoA), methyl coenzyme M reductase (mcrA), and the archaeal 16S rRNA gene. The pmoA library indicated that Methylomonas and Methylocystis predominated in the Sphagnum-covered and Sasa-invaded areas, respectively. The mcrA and 16S rRNA libraries indicated that Methanoregula were abundant methanogens in the Sphagnum-covered area. In the Sasa-invaded area, by contrast, mcrA genes were not detected, and no 16S rRNA clones were affiliated with previously known methanogens. Because the Sasa-invaded area still produced methane, of the various uncultured populations detected, novel euryarchaeotal lineages are candidate methane producers.
authors: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
authors: Takashi
authors: T
authors: Narihiro
authors: Tomoyuki
authors: T
authors: Hori
authors: Osamu
authors: O
authors: Nagata
authors: Tamotsu
authors: T
authors: Hoshino
authors: Isao
authors: I
authors: Yumoto
authors: Yoichi
authors: Y
authors: Kamagata
mesh_terms: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D007564:Japan; D008697:Methane / Q000378:metabolism; D042083:Methylocystaceae / Q000201:enzymology / Q000235:genetics*; D020620:Methylomonas / Q000201:enzymology / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D031787:Sasa / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D012987:Soil; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012987:Soil; D010088:Oxidoreductases; D010105:Oxygenases; C021555:methane monooxygenase; C021572:methyl coenzyme M reductase; D008697:Methane