title: The role of apoptosis in immunosuppression of dogs with demodicosis.
abstract: The aim of the present study was to evaluate the status of apoptosis in peripheral blood leukocytes of dogs with demodicosis. A total of 26 dogs suffering from demodicosis, and positive for Demodex canis mites by skin scraping, participated in the study, 13 with localized demodicosis (LD) and 13 with generalized demodicosis (GD). A further 13 clinically healthy dogs, all of whom were negative for mites upon skin scraping, were used as controls. The dogs with GD revealed significantly higher (P ≤ 0.0001) percentage of leukocytes with externalization of phosphatidylserine (PS) and depolarized mitochondrial membrane potentials (ΔΨm) as compared with the dogs with LD and healthy controls. These dogs also revealed significantly lower values (P ≤ 0.0001) of hematological parameters viz. hemoglobin, total erythrocytes count total leukocytes count, lymphocytes, monocytes and neutrophils. Significantly higher (P ≤ 0.0001) percentages of leukocytes with externalization of PS and depolarized ΔΨm were also found in dogs with LD as compared with the healthy controls. These dogs also revealed significantly lower values of Hb (P ≤ 0.0001), TEC (P=0.025), TLC (P ≤ 0.0001), lymphocytes (P=0.008), monocytes (P ≤ 0.0001) and neutrophils (P=0.03). It is concluded that premature apoptosis of PBL may be implicated in the immunosuppression of the dogs with demodicosis.
authors: Division of Medicine, Indian Veterinary Research Institute, Izatnagar 243 122, Bareilly, UP, India. pshankervet@gmail.com
authors: Shanker K
authors: SK
authors: Singh
authors: Umesh
authors: U
authors: Dimri
authors: Mahesh C
authors: MC
authors: Sharma
authors: Devendra
authors: D
authors: Swarup
authors: Bhaskar
authors: B
authors: Sharma
authors: Hari Om
authors: HO
authors: Pandey
authors: Priyambada
authors: P
authors: Kumari
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007108:Immune Tolerance / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology / Q000502:physiology; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000502:physiology; D008924:Mite Infestations / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D008925:Mites / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000469:parasitology