title: Rosiglitazone enhances the proliferation of neural progenitor cells and inhibits inflammation response after spinal cord injury.
abstract: It has been previously shown that peroxisome proliferators-activated receptor gamma (PPAR-γ) is beneficial for nervous system injury. In present study, we examined the effect of rosiglitazone, a PPAR-γ agonist, on spinal cord injury (SCI) in rats. SCI was induced by dropping a 10g weight rod at a height of 25mm. The animals were randomly divided into vehicle group, rosiglitazone treated group, and G3335 treated group. Locomotor function recovery was evaluated by the Basso-Beattie-Bresnahan locomotor rating scale (BBB scale), NF-κB expression and endogenous neural progenitor cells (NPCs) proliferation and differentiation was assessed by flow cytometry and immunohistochemistry. Compared with the vehicle groups, we found that the rosiglitazone could significantly ameliorate locomotor recovery, reduce NF-κB expression, and increase the proliferation of endogenous NPCs. when the PPAR-γ antagonist was use, these effects were abolished. However, neurons differentiating from endogenous NPCs were inhibited when PPAR-γ was activated. Our results suggest that the activation of PPAR-γ may be a potential alternative treatment for spinal cord injury.
authors: Department of Orthopedics, Institute of Spinal Cord Injury of Sun Yat-sen University, Yanjiangxi Road 107, 510120 Guangzhou, China.
authors: Qing-Qi
authors: QQ
authors: Meng
authors: Xin-Jun
authors: XJ
authors: Liang
authors: Peng
authors: P
authors: Wang
authors: Xiao-Pin
authors: XP
authors: Wang
authors: Jie-Wen
authors: JW
authors: Yang
authors: Yan-Feng
authors: YF
authors: Wu
authors: Hui-Yong
authors: HY
authors: Shen
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents; D034961:Antigens, Nuclear / Q000378:metabolism; D000963:Antimetabolites; D001812:Blood-Brain Barrier / Q000187:drug effects; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D005434:Flow Cytometry; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D007381:Intermediate Filament Proteins / Q000378:metabolism; D009068:Movement / Q000502:physiology; D016328:NF-kappa B / Q000096:biosynthesis / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D058953:Neural Stem Cells / Q000187:drug effects*; D047495:PPAR gamma / Q000819:agonists* / Q000037:antagonists & inhibitors; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D000077154:Rosiglitazone; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000473:pathology; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D034961:Antigens, Nuclear; D000963:Antimetabolites; D007381:Intermediate Filament Proteins; D016328:NF-kappa B; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D047495:PPAR gamma; C546339:Rbfox3 protein, rat; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D001973:Bromodeoxyuridine