title: Evaluation of an ELISA assay for total proinsulin and establishment of reference values during an oral glucose tolerance test in a healthy population.
abstract: OBJECTIVE
  Assessment of the analytical performance of the Total Proinsulin ELISA Kit (Millipore) and determination of reference values.
  
  
  METHODS
  Imprecision, specificity, antibodies interference and reference values in normoglycaemic non-obese adults were determined.
  
  
  RESULTS
  The inter-assay CV is <6.9%, the limits of detection and quantification are 0.2 and 0.6 pmol/L. Molar cross-reactivity of split proinsulins varies from 103 to 92.5%. The interference of anti-(pro)insulin antibodies can be eliminated with the use of polyethylene glycol. The reference values are 2.7-14.2 pmol/L at fasting, 8.5-56.5 pmol/L at T30 min and 11.9-70.5 pmol/L at T120 min during an OGTT.
  
  
  CONCLUSIONS
  The reference values established for this kit, which showed good analytical performances, allow for a better assessment of pathologies associated with increased proinsulinaemia.
authors: Hôpital Robert Debré, laboratoire de biochimie-hormonologie, 48 bd Sérurier, 75019-Paris, France. didier.chevenne@rdb.aphp.fr
authors: Didier
authors: D
authors: Chevenne
authors: Samia
authors: S
authors: Deghmoun
authors: Laurence
authors: L
authors: Coric
authors: Mireille
authors: M
authors: Nicolas
authors: Claire
authors: C
authors: Lévy-Marchal
mesh_terms: D000293:Adolescent; D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005215:Fasting / Q000097:blood; D005260:Female; D005951:Glucose Tolerance Test / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D011384:Proinsulin / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D007328:Insulin; D011933:Reagent Kits, Diagnostic; D011384:Proinsulin