title: Characterization of the 3':5' ratio for reliable determination of RNA quality.
abstract: Determination of RNA quality is a critical first step in obtaining meaningful gene expression data. The PCR-based 3':5' assay is an RNA quality assessment tool. This assay is a simple, fast, and low-cost method of selecting samples for further analysis. However, its practical applications are unexploited primarily because of the absence of an experimental threshold. We show that, by anchoring the 5' assay a specific distance from the 3' end of the sequence and by spacing the 3' at a distance of a number of nucleotides, a cutoff determines whether a sample is suitable for downstream quantification studies.
authors: Mejora Genética Vegetal, Instituto de Agricultura Sostenible, 14080 Córdoba, Spain. jdieramon@ias.csic.es
authors: Jose V
authors: JV
authors: Die
authors: Ángeles
authors: Á
authors: Obrero
authors: Clara I
authors: CI
authors: González-Verdejo
authors: Belén
authors: B
authors: Román
mesh_terms: D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D046913:Medicago truncatula / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis* / Q000592:standards*
chemical_list: D018076:DNA, Complementary; D018749:RNA, Plant