title: Controlled clockwise-counterclockwise motion of the ring-shaped microtubules assembly.
abstract: The microtubule (MT)-kinesin system has been proposed as the building block of biomolecular motor based artificial biomachines. Considerable efforts have been devoted to integrate this system that produced a variety of ordered structures including the ring-shaped MT assembly which is being considered as a promising candidate for the further development of the biomachines. However, lack of proper knowledge that might help tune the direction of motion of ring-shaped microtubule assembly from counterclockwise to clockwise direction, and vice versa, significantly restricted their potential applications. We report our success in controlling the direction of rotational motion of ring-shaped MT assembly by altering the preparation conditions of microtubules. The change in the direction of rotation of MT rings could be interpreted in terms of the accompanied structural rearrangement of the MT lattice. For achieving handedness-regulated efficient biomachines having tunable asymmetric property, our study will be significantly directive.
authors: Faculty of Advanced Life Science, Hokkaido University, Sapporo 060-0810, Japan. gong@mail.sci.hokudai.ac.jp
authors: Akira
authors: A
authors: Kakugo
authors: Arif Md Rashedul
authors: AM
authors: Kabir
authors: Natsuki
authors: N
authors: Hosoda
authors: Kazuhiro
authors: K
authors: Shikinaka
authors: Jian Ping
authors: JP
authors: Gong
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001709:Biotechnology / Q000379:methods*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D019921:Biotinylation; D016547:Kinesins / Q000737:chemistry* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D009038:Motion; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism; D012235:Rhodamines / Q000032:analysis; D012399:Rotation; D013552:Swine; D014404:Tubulin / Q000737:chemistry* / Q000378:metabolism; D014741:Video Recording
chemical_list: D011993:Recombinant Fusion Proteins; D012235:Rhodamines; D014404:Tubulin; D001710:Biotin; D016547:Kinesins