title: Production of camptothecin by hairy roots and regenerated transformed shoots of Ophiorrhiza rugosa var. decumbens.
abstract: Camptothecin (CPT), the derivatives of which are used clinically for the treatment of metastatic colon cancer, is isolated from intact plants that can be subjected to environmental fluctuations. In vitro cultures may be an alternate and continuous source for year-round production of CPT. Since CPT production by undifferentiated cell cultures is low, differentiated tissues such as root cultures may be a viable alternate source for CPT production. Hairy roots were induced in Ophiorriza rugosa, a source of CPT, using Agrobacterium rhizogenes strain LBA9402. The hairy roots, when cultured in light, showed spontaneous regeneration of shoots. Analysis of CPT levels in the hairy roots and in vitro-grown transformed shoots revealed 0.009% d.w. and 0.012% d.w., respectively.
authors: Plant Biotechnology and Secondary Products Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai - 400085, Maharashtra, India.
authors: Suchita
authors: S
authors: Kamble
authors: Roja
authors: R
authors: Gopalakrishnan
authors: Susan
authors: S
authors: Eapen
mesh_terms: D060054:Agrobacterium; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D002166:Camptothecin / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D018520:Plant Shoots / Q000737:chemistry*; D019666:Rubiaceae / Q000737:chemistry*; D046509:Tissue Culture Techniques
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D002166:Camptothecin