title: Biomass production and fatty acid profile of a Scenedesmus rubescens-like microalga.
abstract: This investigation examined the effects of nitrogen-phosphate combined deficiency on the biomass yield, fatty acid methyl esters (FAME) production and composition from Scenedesmus rubescens-like microalga. A 15-day indoor culture was performed as a 3 × 3 factorial design (NaNO(3) levels: 3, 10 and 20mM; KH(2)PO(4) levels: 20, 50 and 150 μM). The algae grown under medium nitrogen concentration (10mM) and high phosphate concentration (150 μM) reached the highest biomass (1223.5 ± 152.5mg/L). Both nitrogen and phosphate had a significant influence on the FAME yield (P<0.05 and P<0.0001, respectively). The FAME yield from algae grown under low nitrogen (3mM) and phosphate concentration (20 μM) increased throughout the experiment and the highest FAME yield (42.2 ± 2.5% of AFDW) as well as C16 and C18 content (95.8 ± 1.6% of AFDW) was achieved under these conditions. Algae grown under medium nitrogen concentration (10mM) and low phosphate concentration (20 μM) had the highest FAME productivity (426.0mg/L ± 135.0mg/L). Thus, the lower nitrogen concentration (3mM-10mM) and low phosphate concentration (20 μM) would be an optimal combination tested to produce the most FAME from S. rubescens-like algae.
authors: Florida Institute of Technology, 150 W. University Blvd., Melbourne, FL 32901, USA. ytan2009@my.fit.edu
authors: Yixin
authors: Y
authors: Tan
authors: Junda
authors: J
authors: Lin
mesh_terms: D018533:Biomass; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D009584:Nitrogen / Q000172:deficiency / Q000494:pharmacology; D010710:Phosphates / Q000172:deficiency / Q000494:pharmacology; D044444:Scenedesmus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D004952:Esters; D005227:Fatty Acids; D010710:Phosphates; D009584:Nitrogen