title: Ethanol production from selected lignocellulosic hydrolysates by genome shuffled strains of Scheffersomyces stipitis.
abstract: Two genome-shuffled Scheffersomyces stipitis strains, GS301 and GS302, exhibiting improved tolerance to hardwood spent sulphite liquor, were tested for growth and fermentation performance on three wood hydrolysates: (a) steam-pretreated enzymatically hydrolyzed poplar hydrolysate from Mascoma Canada, (b) steam pretreated poplar hydrolysate from University of British Columbia Forest Products Biotechnology Laboratory, and (c) mixed hardwoods pre-hydrolysate from FPInnovations (FPI). In the FPI hydrolysate, the wild type (WT) died off within 25 h, while GS301 and GS302 survived beyond 100 h. In fermentation tests, GS301 and GS302 completely utilized glucose and xylose in each hydrolysate and produced 0.39-1.4% (w/v) ethanol. In contrast, the WT did not utilize or poorly utilized glucose and xylose and produced non-detectable to trace amounts of ethanol. The results demonstrated cross tolerance of the mutants to inhibitors in three different wood hydrolysates and reinforced the utility of mating-based genome shuffling approach in industrial yeast strain improvement.
authors: School of Environmental Sciences, University of Guelph, Guelph, Ontario, Canada.
authors: Paramjit K
authors: PK
authors: Bajwa
authors: Chetsada
authors: C
authors: Phaenark
authors: Nicola
authors: N
authors: Grant
authors: Xiao
authors: X
authors: Zhang
authors: Michael
authors: M
authors: Paice
authors: Vincent J J
authors: VJ
authors: Martin
authors: Jack T
authors: JT
authors: Trevors
authors: Hung
authors: H
authors: Lee
mesh_terms: D032621:DNA Shuffling; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D016681:Genome, Fungal / Q000235:genetics*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D014934:Wood / Q000737:chemistry; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D000431:Ethanol; D008031:Lignin