title: Oxidative biomarkers in leaf tissue of barley seedlings in response to aluminum stress.
abstract: Cellular responses to Al-stress in Hordeum vulgare seedling bioassay were evaluated with an objective to identify the possible biomarkers in leaf tissue that would be best suited to biomonitor aluminum (Al) in the environment. Germinating seeds were treated with different concentrations of AlCl(3) at pH 4.5 for 12h. Al-uptake and accumulation in root and leaf, generation of reactive oxygen species (ROS: O(2)(-), H(2)O(2) and ()OH), cell death, activity of antioxidant enzymes: catalase, superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, lipid peroxidation, protein oxidation, DNase activity and DNA damage were measured in leaf tissue of the seedlings on day 6 after treatment. The above parameters assessed in leaf tissue that followed a dose-response exhibited significant correlation with concentration of Al(3+) in experimental solution as well as in root tissue. The findings underscored the sensitivity as well as potential of Hordeum vulgare seedling bioassay for biomonitoring of Al in the ambient environment.
authors: Department of Botany, Berhampur University, Berhampur, India.
authors: V
authors: V
authors: Mohan Murali Achary
authors: Anita Rani
authors: AR
authors: Patnaik
authors: Brahma B
authors: BB
authors: Panda
mesh_terms: D000535:Aluminum / Q000378:metabolism / Q000633:toxicity*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016923:Cell Death; D001467:Hordeum / Q000187:drug effects / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D000535:Aluminum; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase