title: Periodontal regeneration following implantation of cementum and periodontal ligament-derived cells.
abstract: OBJECTIVE
  The periodontal regeneration of bone defects is often unsatisfactory and could be largely improved by cell therapy. Therefore, the purpose of this study was to evaluate the regenerative potential of implanting canine cementum-derived cells (CDCs) and canine periodontal ligament-derived cells (PDLDCs) in experimentally created periodontal intrabony defects in beagle dogs.
  
  
  METHODS
  Cells were obtained from premolars extracted from four beagle dogs. Three-wall intrabony periodontal defects, 3 mm wide and 4 mm deep, were surgically created in their second and fourth premolars and plaque was allowed to accumulate. Once the defects were surgically debrided, periodontal regeneration was attempted by random implantation of collagen sponges embedded with 750,000 CDCs, 750,000 PDLDCs or culture medium. After 3 mo of healing, specimens were obtained and periodontal regenerative outcomes were assessed histologically and histometrically.
  
  
  RESULTS
  The histological analysis showed that a minimal amount of new cementum was formed in the control group (1.56 ± 0.39 mm), whereas in both test groups, significantly higher amounts of new cementum were formed (3.98 ± 0.59 mm in the CDC group and 4.07 ± 0.97 mm in the PDLDC group). The test groups also demonstrated a larger dimension of new connective tissue, resulting in a significantly more coronal level of histological attachment.
  
  
  CONCLUSIONS
  This proof-of-principle study suggests that cellular therapy, in combination with a collagen sponge, promoted periodontal regeneration in experimental intrabony periodontal defects.
authors: Institute of Molecular Biology and Genetics, University of Valladolid and Spanish Research Council, Valladolid, Spain. javilds@hotmail.com
authors: J
authors: J
authors: Nuñez
authors: S
authors: S
authors: Sanz-Blasco
authors: F
authors: F
authors: Vignoletti
authors: F
authors: F
authors: Muñoz
authors: H
authors: H
authors: Arzate
authors: C
authors: C
authors: Villalobos
authors: L
authors: L
authors: Nuñez
authors: R G
authors: RG
authors: Caffesse
authors: M
authors: M
authors: Sanz
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D001641:Bicuspid / Q000473:pathology; D018929:Cell Culture Techniques; D002469:Cell Separation; D002470:Cell Survival; D017690:Cell Transplantation; D025021:Cementogenesis / Q000502:physiology; D003094:Collagen; D003238:Connective Tissue / Q000473:pathology; D003646:Debridement; D003739:Dental Cementum / Q000166:cytology*; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D008297:Male; D010513:Periodontal Ligament / Q000166:cytology*; D011897:Random Allocation; D013357:Subgingival Curettage; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D003094:Collagen