title: Assessment of 1,2-propanediol (PrOH) genotoxicity on mouse oocytes by comet assay.
abstract: OBJECTIVE
  To assess the genotoxicity of 1,2-propanediol (PrOH) on mouse oocytes by comet assay.
  
  
  METHODS
  In vitro assay using murine model.
  
  
  METHODS
  Biogenotoxicology research laboratory.
  
  
  METHODS
  CD1 female mice.
  
  
  METHODS
  Three 40-oocyte groups were exposed to different PrOH concentrations (5%, 7.5%, and 15%). Each concentration was tested during both long and short exposures (1-2 hours and 1-5 minutes) in comparison with control groups. DNA damage was evaluated by a single-cell gel electrophoresis assay, also called "comet assay," and analyzed with Komet software.
  
  
  METHODS
  DNA damage was quantified as Olive tail moment (OTM). Interpretation was done on OTM with the use of χ(2).
  
  
  RESULTS
  High PrOH concentrations (7.5% and 15%) induced significant DNA damage on mouse oocytes. The OTM χ(2) values were 4.16 ± 0.40 and 6.80 ± 0.4 with 7.5% PrOH at 1 and 2 hours, respectively, 24.35 ± 1.60 with 15% at 1 hour, and for 2h at 15% the DNA damage was too drastic to calculate OTM χ(2). After 1 and 5 minutes, the OTM χ(2) values were, respectively, 5.19 ± 0.26 and 6.06 ± 0.42 with 7.5%, and 7.53 ± 0.33 and 16.81 ± 0.67 with 15%.
  
  
  CONCLUSIONS
  High concentrations of PrOH (7.5% and 15%) induced significant DNA damage on mouse oocytes, whatever the exposure duration. These results should be interpreted with caution, because additional data are needed to evaluate PrOH genotoxicity and DNA oocyte reparation after exposure to high PrOH concentrations.
authors: Laboratoire de Biogénotoxicologie et Mutagenèse Environnementale, Fédération de Recherche CNRS 3098 ECCOREV, Facultés de Médecine et Pharmacie, Université de Méditerranée, Marseille, France.
authors: Anais
authors: A
authors: Berthelot-Ricou
authors: Jeanne
authors: J
authors: Perrin
authors: Carole
authors: C
authors: di Giorgio
authors: Michel
authors: M
authors: de Meo
authors: Alain
authors: A
authors: Botta
authors: Blandine
authors: B
authors: Courbiere
mesh_terms: D000818:Animals; D020552:Comet Assay / Q000379:methods*; D004249:DNA Damage / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006736:Horses; D051379:Mice; D009153:Mutagens / Q000633:toxicity*; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D019946:Propylene Glycol / Q000633:toxicity*
chemical_list: D009153:Mutagens; D019946:Propylene Glycol