title: Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis.
abstract: Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods; however, few synthetic antigens with sufficient sensitivity and specificity are available. Here we evaluated a glycan microarray approach to select for synthetic glycan antigens that could be used for serodiagnosis of parasitic infections. Using a glycan array containing over 250 different glycan antigens, we identified GalNAcβ1-4(Fucα1-3)GlcNAc-R (LDNF) as a glycan antigen that is recognized by antibodies from Trichinella-infected individuals. We synthesized a neoglycoconjugate, consisting of five LDNF molecules covalently coupled to bovine serum albumin (BSA), and used this neoglycoconjugate as an antigen to develop a highly sensitive total-Ig ELISA for serological screening of trichinellosis. The results indicate that glycan microarrays constitute a promising technology for fast and specific identification of parasite glycan antigens to improve serodiagnosis of different parasitic infections, either using an ELISA format, or parasite-specific glycan arrays.
authors: Centre for Infectious Disease Control Netherlands, National Institute of Public Health and Environment, Bilthoven, The Netherlands.
authors: Carmen
authors: C
authors: Aranzamendi
authors: Boris
authors: B
authors: Tefsen
authors: Montse
authors: M
authors: Jansen
authors: Lorena
authors: L
authors: Chiumiento
authors: Fabrizio
authors: F
authors: Bruschi
authors: Titia
authors: T
authors: Kortbeek
authors: David F
authors: DF
authors: Smith
authors: Richard D
authors: RD
authors: Cummings
authors: Elena
authors: E
authors: Pinelli
authors: Irma
authors: I
authors: Van Die
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives* / Q000276:immunology; D008297:Male; D011134:Polysaccharides / Q000276:immunology*; D040081:Protein Array Analysis; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; C488538:N-acetylgalactosaminyl-1-4-(fucosyl-1,3)-N-acetylglucosamine; D011134:Polysaccharides; D007785:Lactose