title: Bioassay-directed isolation of active compounds with antiyeast activity from a Cassia fistula seed extract.
abstract: OBJECTIVE
  Cassia fistula L belongs to the family Leguminosae, and it is one of the most popular herbal products in tropical countries. C. fistula seeds have been used as a herbal medicine and have pharmacological activity which includes anti-bacterial, anti-fungal, and antioxidant properties. The goal of this study was to identify compounds from C. fistula seeds which are responsible for anti-Candida albicans activity using bioassay-directed isolation.
  
  
  RESULTS
  The preliminary phytochemical screening of the plant seed revealed the presence of anthraquinones, flavonoids, saponins, tannins and terpenoids. The isolation of active compounds was carried out in four steps: multiple extractions, fractionation using column chromatography and purification using preparative thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). The structure of separated compounds was determined on the basis of mass spectrometry data. One compound was identified is roseanone.
  
  
  CONCLUSIONS
  The MS analysis on the active fraction from seed extract of C. fistula confirmed the presence of roseanone with antiyeast activity.
authors: Biological Program, School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia.
authors: Subramanion L
authors: SL
authors: Jothy
authors: Zuraini
authors: Z
authors: Zakaria
authors: Yeng
authors: Y
authors: Chen
authors: Yee Ling
authors: YL
authors: Lau
authors: Lachimanan Yoga
authors: LY
authors: Latha
authors: Lai Ngit
authors: LN
authors: Shin
authors: Sreenivasan
authors: S
authors: Sasidharan
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001681:Biological Assay; D002176:Candida albicans / Q000187:drug effects / Q000648:ultrastructure; D002366:Cassia / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D010636:Phenols; D010936:Plant Extracts; D000432:Methanol