title: Comparative analysis of putative pathogenesis-related gene expression in two Rhizoctonia solani pathosystems.
abstract: Rhizoctonia solani, teleomorph Thanatephorus cucumeris, is a polyphagous necrotrophic plant pathogen of the Basidiomycete order that is split into 14 different anastomosis groups (AGs) based on hyphal interactions and host range. In this investigation, quantitative real-time PCR (qRT-PCR) techniques were used to determine potential pathogenicity factors of R. solani in the AG1-IA/rice and AG3/potato pathosystems. These factors were identified by mining for sequences of pathogen origin in a library of rice tissue infected with R. solani AG1-IA and comparing these sequences against the recently released R. solani AG3 genome. Ten genes common to both AGs and two specific to AG1-IA were selected for expression analysis by qRT-PCR. Results indicate that a number of genes are similarly expressed by AG1 and AG3 during the early stages of pathogenesis. Grouping of these pathogenicity factors based on relatedness of expression profiles suggests three key events are involved in R. solani pathogenesis: early host contact and infiltration, adjustment to the host environment, and pathogen proliferation through necrotic tissue. Further studies of the pathogenesis-associated genes identified in this project will enable more precise elucidation of the molecular mechanisms that allow for the widespread success of R. solani as a phytopathogen and allow for more targeted, effective methods of management.
authors: School of Biology and Ecology, University of Maine, Orono, ME 04469-5735, USA.
authors: Renee
authors: R
authors: Rioux
authors: Harish
authors: H
authors: Manmathan
authors: Pratibha
authors: P
authors: Singh
authors: Benildo
authors: B
authors: de los Reyes
authors: Yulin
authors: Y
authors: Jia
authors: Stellos
authors: S
authors: Tavantzis
mesh_terms: D020224:Expressed Sequence Tags; D015966:Gene Expression Regulation, Fungal; D015723:Gene Library; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012232:Rhizoctonia / Q000145:classification / Q000235:genetics / Q000472:pathogenicity