title: Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars.
abstract: We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and 3× improvement in the signal intensity for fixed mouse tissues at depths of 70 μm.
authors: Jack Baskin School of Engineering, University of California, Santa Cruz, Santa Cruz, California 95064, USA. taoxd@soe.ucsc.edu
authors: Xiaodong
authors: X
authors: Tao
authors: Oscar
authors: O
authors: Azucena
authors: Min
authors: M
authors: Fu
authors: Yi
authors: Y
authors: Zuo
authors: Diana C
authors: DC
authors: Chen
authors: Joel
authors: J
authors: Kubby
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation*; D055096:Optical Devices; D038761:Photobleaching
chemical_list: D049452:Green Fluorescent Proteins