title: Glycosphingolipid synthesis employing a combination of recombinant glycosyltransferases and an endoglycoceramidase glycosynthase.
abstract: Glycosynthase mutants of Rhodococcus sp. endo-glycoceramidase II efficiently synthesize complex glycosphingolipids. Glycosyl fluoride donors may be assembled via sequential glycosyltransferase-catalysed glycosylation of lactosyl fluoride. Alternatively, lactosyl fluoride may be coupled to sphingosine prior to subsequent glycosylation steps.
authors: Department of Chemistry and Centre for High-Throughput Biology, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, Canada V6T 1Z1.
authors: Jamie R
authors: JR
authors: Rich
authors: Anna-Maria
authors: AM
authors: Cunningham
authors: Michel
authors: M
authors: Gilbert
authors: Stephen G
authors: SG
authors: Withers
mesh_terms: D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D006028:Glycosphingolipids / Q000096:biosynthesis* / Q000737:chemistry; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012240:Rhodococcus / Q000201:enzymology
chemical_list: D006028:Glycosphingolipids; D011994:Recombinant Proteins; C084793:lactosyl fluoride; D016695:Glycosyltransferases; D006026:Glycoside Hydrolases; C050427:endoglycoceramidase; D007785:Lactose