title: Novel inducers of the envelope stress response BaeSR in Salmonella Typhimurium: BaeR is critically required for tungstate waste disposal.
abstract: The RpoE and CpxR regulated envelope stress responses are extremely important for Salmonella Typhimurium to cause infection in a range of hosts. Until now the role for BaeSR in both the Salmonella Typhimurium response to stress and its contribution to infection have not been fully elucidated. Here we demonstrate stationary phase growth, iron and sodium tungstate as novel inducers of the BaeRregulon, with BaeR critically required for Salmonella resistance to sodium tungstate. We show that functional overlap between the resistance nodulation-cell division (RND) multidrug transporters, MdtA, AcrD and AcrB exists for the waste disposal of tungstate from the cell. We also point to a role for enterobactinsiderophores in the protection of enteric organisms from tungstate, akin to the scenario in nitrogen fixing bacteria. Surprisingly, BaeR is the first envelope stress response pathway investigated in S. Typhimurium that is not required for murine typhoid in either ity(S) or ity(R) mouse backgrounds. BaeR is therefore either required for survival in larger mammals such as pigs or calves, an avian host such as chickens, or survival out with the host altogether where Salmonella and related enterics must survive in soil and water.
authors: School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, United Kingdom. c.appia-ayme@uea.ac.uk
authors: Corinne
authors: C
authors: Appia-Ayme
authors: Elaine
authors: E
authors: Patrick
authors: Matthew J
authors: MJ
authors: Sullivan
authors: Mark J
authors: MJ
authors: Alston
authors: Sarah J
authors: SJ
authors: Field
authors: Manal
authors: M
authors: AbuOun
authors: Muna F
authors: MF
authors: Anjum
authors: Gary
authors: G
authors: Rowley
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D004351:Drug Resistance / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics* / Q000502:physiology; D013312:Stress, Physiological; D017973:Tungsten Compounds / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D011494:Protein Kinases; D017973:Tungsten Compounds; C025399:sodium tungstate(VI); C525552:BaeS protein, Salmonella typhimurium; C045951:tungstate