title: The impact of dendrimer-grafted modifications to model silicon surfaces on protein adsorption and bacterial adhesion.
abstract: In the oral cavity, omnipresent salivary protein films (pellicle) mediate bacterial adhesion and biofilm formation on natural tissues as well as on artificial implant surfaces, which may cause serious infectious diseases like periimplantitis. The purpose of this in vitro study was to investigate the adsorption/desorption behaviour of human saliva on model surfaces grafted with polyamidoamine (PAMAM) dendrimer molecules compared to self-assembled monolayers (SAMs) exhibiting the same terminal functions (-NH(2), -COOH) by two complementary analytical methods. Furthermore, the role of saliva conditioning of PAMAM and analogous SAM modifications on the adhesion of Streptococcus gordonii DL1, an early oral colonizer, was investigated. In contrast to SAMs, PAMAM-grafted surfaces showed reduced streptococcal adherence in the absence of pre-adsorbed saliva similar to the level obtained for poly(ethylene glycol) (PEG) coatings. Moreover, coatings of PAMAM-NH(2) maintained their bacteria-repellent behaviour even after saliva-conditioning. As a general outcome, it was found that lower amounts of protein adsorbed on PAMAM coatings than on analogous SAMs. Since this study demonstrates that covalently bound PAMAM dendrimers can modulate the oral bacterial response, this approach has significant potential for the development of anti-adhesive biomaterial surfaces that are conditioned with proteinaceous films.
authors: University Hospital Tuebingen, Department of Prosthetic Dentistry, Section Medical Materials and Technology, Osianderstr. 2-8, 72076 Tuebingen, Germany.
authors: Mirjam
authors: M
authors: Eichler
authors: Verena
authors: V
authors: Katzur
authors: Lutz
authors: L
authors: Scheideler
authors: Michael
authors: M
authors: Haupt
authors: Juergen
authors: J
authors: Geis-Gerstorfer
authors: Gottfried
authors: G
authors: Schmalz
authors: Stefan
authors: S
authors: Ruhl
authors: Rainer
authors: R
authors: Müller
authors: Frank
authors: F
authors: Rupp
mesh_terms: D000327:Adsorption / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D050091:Dendrimers / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007526:Isoelectric Point; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D058525:Quartz Crystal Microbalance Techniques; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D012825:Silicon / Q000494:pharmacology*; D055672:Static Electricity; D054773:Streptococcus gordonii / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013499:Surface Properties / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D050091:Dendrimers; C104700:PAMAM Starburst; D012471:Salivary Proteins and Peptides; D014867:Water; D012825:Silicon