title: Cell patterning using a template of microstructured organosilane layer fabricated by vacuum ultraviolet light lithography.
abstract: Micropatterning techniques have become increasingly important in cellular biology. Cell patterning is achieved by various methods. Photolithography is one of the most popular methods, and several light sources (e.g., excimer lasers and mercury lamps) are used for that purpose. Vacuum ultraviolet (VUV) light that can be produced by an excimer lamp is advantageous for fabricating material patterns, since it can decompose organic materials directly and efficiently without photoresist or photosensitive materials. Despite the advantages, applications of VUV light to pattern biological materials are few. We have investigated cell patterning by using a template of a microstructured organosilane layer fabricated by VUV lithography. We first made a template of a microstructured organosilane layer by VUV lithography. Cell adhesive materials (poly(d-lysine) and polyethyleneimine) were chemically immobilized on the organosilane template, producing a cell adhesive material pattern. Primary rat cardiac and neuronal cells were successfully patterned by culturing them on the pattern substrate. Long-term culturing was attained for up to two weeks for cardiac cells and two months for cortex cells. We have discussed the reproducibility of cell patterning and made suggestions to improve it.
authors: Advanced Industrial Science and Technology (AIST), 2-17-2-1, Tsukisamu-Higashi, Sapporo, 062-8517 Japan.
authors: Munehiro
authors: M
authors: Yamaguchi
authors: Koji
authors: K
authors: Ikeda
authors: Masaaki
authors: M
authors: Suzuki
authors: Ai
authors: A
authors: Kiyohara
authors: Suguru N
authors: SN
authors: Kudoh
authors: Kyoko
authors: K
authors: Shimizu
authors: Toshio
authors: T
authors: Taira
authors: Daisuke
authors: D
authors: Ito
authors: Tsutomu
authors: T
authors: Uchida
authors: Kazutoshi
authors: K
authors: Gohara
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D008958:Models, Molecular; D015394:Molecular Structure; D009206:Myocardium / Q000166:cytology*; D009474:Neurons / Q000737:chemistry*; D051381:Rats; D012821:Silanes / Q000737:chemistry*; D013499:Surface Properties; D014466:Ultraviolet Rays; D014618:Vacuum
chemical_list: D012821:Silanes