title: Post-synthetic modification of plant cell walls by expression of microbial hydrolases in the apoplast.
abstract: The systematic creation of defined cell wall modifications in the model plant Arabidopsis thaliana by expression of microbial hydrolases with known specific activities is a promising approach to examine the impacts of cell wall composition and structure on both plant fitness and cell wall recalcitrance. Moreover, this approach allows the direct evaluation in living plants of hydrolase specificity, which can differ from in vitro specificity. To express genes encoding microbial hydrolases in A. thaliana, and target the hydrolases to the apoplast compartment, we constructed an expression cassette composed of the Cauliflower Mosaic Virus 35S RNA promoter, the A. thaliana β-expansin signal peptide, and the fluorescent marker protein YFP. Using this construct we successfully introduced into Colombia-0 plants three Aspergillus nidulans hydrolases, β-xylosidase/α-arabinosidase, feruloyl esterase, acetylxylan esterase, and a Xanthomonas oryzae putative a-L: -arabinofuranosidase. Fusion with YFP permitted quick and easy screening of transformants, detection of apoplastic localization, and protein size confirmation. Compared to wild-type Col-0, all transgenic lines showed a significant increase in the corresponding hydrolytic activity in the apoplast and changes in cell wall composition. Examination of hydrolytic activity in the transgenic plants also showed, for the first time, that the X. oryzae gene indeed encoded an enzyme with α-L: -arabinofuranosidase activity. None of the transgenic plants showed a visible phenotype; however, the induced compositional changes increased the degradability of biomass from plants expressing feruloyl esterase and β-xylosidase/α-arabinosidase. Our results demonstrate the viability of creating a set of transgenic A. thaliana plants with modified cell walls to use as a toolset for investigation of how cell wall composition contributes to recalcitrance and affects plant fitness.
authors: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors: Gennady
authors: G
authors: Pogorelko
authors: Oksana
authors: O
authors: Fursova
authors: Ming
authors: M
authors: Lin
authors: Eric
authors: E
authors: Pyle
authors: Johanna
authors: J
authors: Jass
authors: Olga A
authors: OA
authors: Zabotina
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000648:ultrastructure; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D017796:Caulimovirus / Q000235:genetics; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000032:analysis; D006867:Hydrolases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000032:analysis; D030821:Plants, Genetically Modified / Q000648:ultrastructure*; D011401:Promoter Regions, Genetic; D013379:Substrate Specificity; D014974:Xanthomonas / Q000201:enzymology / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D006867:Hydrolases