title: Uptake of 17β-estradiol and biomarker responses in brown trout (Salmo trutta) exposed to pulses.
abstract: In streams, chemicals such as 17β-estradiol (E2) are likely to occur in pulses. We investigated uptake and biomarker responses in juvenile brown trout (Salmo trutta) of 3- or 6-h pulses of concentrations up to 370 ng E2 L(-1). Uptake by the fish was estimated from disappearance of E2 from tank water. A single 6-h pulse of 370 ng E2 L(-1) increased the plasma vitellogenin concentration, liver Erα- and vitellogenin-mRNA. Exposure to 150-160 ng E2 L(-1) for 6 h increased vitellogenin in one experiment but not in another. Two 6-h pulses had a larger effect one pulse. Brown trout in the size range 24-74 g took up E2 linearly with time and exposure concentration with a concentration ratio rate of 20.2 h(-1). In conclusion, the threshold for induction of estrogenic effects in juvenile brown trout at short term pulse exposure appears to be in the range 150-200 ng E2 L(-1).
authors: Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense, Denmark.
authors: Jacob J G
authors: JJ
authors: Knudsen
authors: Henrik
authors: H
authors: Holbech
authors: Steffen S
authors: SS
authors: Madsen
authors: Poul
authors: P
authors: Bjerregaard
mesh_terms: D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000378:metabolism; D004781:Environmental Exposure; D004958:Estradiol / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007700:Kinetics; D008297:Male; D014337:Trout / Q000235:genetics / Q000378:metabolism*; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004958:Estradiol