title: Insight from the draft genome of Dietzia cinnamea P4 reveals mechanisms of survival in complex tropical soil habitats and biotechnology potential.
abstract: The draft genome of Dietzia cinnamea strain P4 was determined using pyrosequencing. In total, 428 supercontigs were obtained and analyzed. We here describe and interpret the main features of the draft genome. The genome contained a total of 3,555,295 bp, arranged in a single replicon with an average G+C percentage of 70.9%. It revealed the presence of complete pathways for basically all central metabolic routes. Also present were complete sets of genes for the glyoxalate and reductive carboxylate cycles. Autotrophic growth was suggested to occur by the presence of genes for aerobic CO oxidation, formate/formaldehyde oxidation, the reverse tricarboxylic acid cycle and the 3-hydropropionate cycle for CO(2) fixation. Secondary metabolism was evidenced by the presence of genes for the biosynthesis of terpene compounds, frenolicin, nanaomycin and avilamycin A antibiotics. Furthermore, a probable role in azinomycin B synthesis, an important product with antitumor activity, was indicated. The complete alk operon for the degradation of n-alkanes was found to be present, as were clusters of genes for biphenyl ring dihydroxylation. This study brings new insights in the genetics and physiology of D. cinnamea P4, which is useful in biotechnology and bioremediation.
authors: Microbial Ecology Laboratory, Department of Microbial Ecology, CEES, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands.
authors: Luciano
authors: L
authors: Procópio
authors: Vanessa M
authors: VM
authors: Alvarez
authors: Diogo A
authors: DA
authors: Jurelevicius
authors: Lars
authors: L
authors: Hansen
authors: Søren J
authors: SJ
authors: Sørensen
authors: Janine S
authors: JS
authors: Cardoso
authors: Marcelo
authors: M
authors: Pádula
authors: Alvaro C
authors: AC
authors: Leitão
authors: Lucy
authors: L
authors: Seldin
authors: Jan Dirk
authors: JD
authors: van Elsas
mesh_terms: D000192:Actinomycetales / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology; D017753:Ecosystem; D016680:Genome, Bacterial; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010802:Phylogeny; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins