title: Role of Sch9 in regulating Ras-cAMP signal pathway in Saccharomyces cerevisiae.
abstract: In Saccharomyces cerevisiae PKA plays a major role in regulating cell growth, metabolism, and stress resistance. We report that Sch9 regulates PKA directly and SCH9 deletion enhances PKA activity by showing that: (1) Bcy1 predominately localized in the nucleus in glycerol-grown sch9Δ cells; (2) large part of the catalytic subunits of PKA transferred from the nucleus to the cytoplasm in sch9Δ cells; (3) higher protein stability of Tpk2 resulted in higher protein level of Tpk2 in sch9Δ than in wild type cells. Our investigations suggest that Sch9 regulates phosphorylation of Bcy1. We also observed hyper-phosphorylation of Cdc25 in sch9Δ, in contrast to the tpk2Δ and tpk2Δsch9Δ mutants, suggesting that feedback inhibition of PKA on Cdc25 is through Tpk2.
authors: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China. zhangaili@tju.edu.cn
authors: Aili
authors: A
authors: Zhang
authors: Yubao
authors: Y
authors: Shen
authors: Wenxuan
authors: W
authors: Gao
authors: Jian
authors: J
authors: Dong
mesh_terms: D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*; D020666:ras-GRF1 / Q000235:genetics / Q000378:metabolism
chemical_list: C542351:CDC25 protein, S cerevisiae; D021122:Protein Subunits; D029701:Saccharomyces cerevisiae Proteins; D020666:ras-GRF1; D000242:Cyclic AMP; D017346:Protein Serine-Threonine Kinases; C530964:SCH9 protein, S cerevisiae; D017868:Cyclic AMP-Dependent Protein Kinases; C457179:TPK2 protein, S cerevisiae; D018631:ras Proteins; D005947:Glucose; D005990:Glycerol