title: Molecularly cloned SHIV-CN97001: a replication-competent, R5 simian/human immunodeficiency virus containing env of a primary Chinese HIV-1 clade C isolate.
abstract: BACKGROUND
  The increasing prevalence of human immunodeficiency virus type 1 (HIV-1) subtype C infection worldwide calls for efforts to develop a relevant animal model for evaluating AIDS candidate vaccines. In China, the prevalent HIV strains comprise a circulating recombinant form, BC (CRF07_BC), in which the envelope belongs to subtype C.
  
  
  METHODS
  To evaluate potential AIDS vaccines targeting Chinese viral strains in non-human primate models, we constructed a simian/human immunodeficiency virus (SHIV) carrying most of the envelope sequence of a primary HIV-1 clade C strain isolated from an HIV-positive intravenous drug user from YunNan province in China. Furthermore, to determine whether in vivo adaptation would enhance the infectivity of SHIV-CN97001, the parental infectious strain was serially passaged through eight Chinese rhesus macaques.
  
  
  RESULTS
  Infection of six Chinese rhesus macaques with SHIV-CN97001 resulted in a low level of viremia and no significant alteration in CD4+ T-lymphocyte counts. However, the hallmarks of SHIV infectivity developed gradually, as shown by the increasingly elevated peak viremia with each passage.
  
  
  CONCLUSIONS
  These findings establish that the R5-tropic SHIV-CN97001/Chinese rhesus macaque model should be very useful for the evaluation of HIV-1 subtype C vaccines in China.
authors: National Center for AIDS/STD Control and prevention, China CDC, Beijing, China.
authors: Qiang
authors: Q
authors: Liu
authors: Yue
authors: Y
authors: Li
authors: GuiBo
authors: G
authors: Yang
authors: JieJie
authors: J
authors: Dai
authors: Ruth M
authors: RM
authors: Ruprecht
authors: Yiming
authors: Y
authors: Shao
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000821:virology; D000818:Animals; D018791:CD4 Lymphocyte Count / Q000662:veterinary; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002678:Chimera; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D005434:Flow Cytometry / Q000662:veterinary; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D006801:Humans; D007275:Injections, Intravenous / Q000662:veterinary; D008253:Macaca mulatta; D011533:Proviruses / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D019713:Receptors, CCR5 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012692:Serial Passage / Q000662:veterinary; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D014766:Viremia / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000662:veterinary
chemical_list: D015686:Gene Products, env; D019713:Receptors, CCR5