title: Interactions of a Pop5/Rpp1 heterodimer with the catalytic domain of RNase MRP.
abstract: Ribonuclease (RNase) MRP is a multicomponent ribonucleoprotein complex closely related to RNase P. RNase MRP and eukaryotic RNase P share most of their protein components, as well as multiple features of their catalytic RNA moieties, but have distinct substrate specificities. While RNase P is practically universally found in all three domains of life, RNase MRP is essential in eukaryotes. The structural organizations of eukaryotic RNase P and RNase MRP are poorly understood. Here, we show that Pop5 and Rpp1, protein components found in both RNase P and RNase MRP, form a heterodimer that binds directly to the conserved area of the putative catalytic domain of RNase MRP RNA. The Pop5/Rpp1 binding site corresponds to the protein binding site in bacterial RNase P RNA. Structural and evolutionary roles of the Pop5/Rpp1 heterodimer in RNases P and MRP are discussed.
authors: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA16802, USA.
authors: Anna
authors: A
authors: Perederina
authors: Elena
authors: E
authors: Khanova
authors: Chao
authors: C
authors: Quan
authors: Igor
authors: I
authors: Berezin
authors: Olga
authors: O
authors: Esakova
authors: Andrey S
authors: AS
authors: Krasilnikov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D020134:Catalytic Domain; D004722:Endoribonucleases / Q000737:chemistry / Q000378:metabolism*; D020035:Holoenzymes / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D055503:Protein Multimerization; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D002352:Carrier Proteins; D020035:Holoenzymes; C562233:Pop5 protein, S cerevisiae; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D004722:Endoribonucleases; C108353:RPP1 protein, S cerevisiae