title: Coordination of carbon fixation and nitrogen metabolism in Salicornia europaea under salinity: Comparative proteomic analysis on chloroplast proteins.
abstract: Halophyte, like Salicornia europaea, could make full use of marginal saline land for carbon fixation. How the photosynthesis of S. europaea is regulated under high salinity implicates a significant aspect to exploit this pioneer plant in future. Measurement of photosynthesis parameters demonstrated the reduction of photosynthesis for the 0 and 800 mM NaCl treated plants are more likely due to non-stomatal limitation, which might be caused by changes in the enzymes associated with photosynthesis. Different salinity induced ultrastructure changes other than photosynthetic apparatus damage, suggesting the photosynthesis of S. europaea might be affected via biochemical regulation. Comparative proteomics analysis of chloroplast proteins by 2-D gel electrophoresis reproducibly detected 90 differentially expressed proteins, among which 66 proteins were identified by nanoLC MS/MS. Further study of thylakoid membrane proteins by Blue-Native PAGE proved the increase in abundance of light reaction proteins under salinity. Analysis of gene expression patterns of 12 selected proteins provides evidence for the correlations between transcription and proteomics data. Based on our results, a putative model of photosynthesis regulatory network figured out proper coordination of carbon fixation and nitrogen metabolism in chloroplast of S. europaea under salinity, which provided subcellular level insight into salt tolerance mechanism in S. europaea.
authors: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P. R. China.
authors: Pengxiang
authors: P
authors: Fan
authors: Juanjuan
authors: J
authors: Feng
authors: Ping
authors: P
authors: Jiang
authors: Xianyang
authors: X
authors: Chen
authors: Hexigeduleng
authors: H
authors: Bao
authors: Lingling
authors: L
authors: Nie
authors: Dan
authors: D
authors: Jiang
authors: Sulian
authors: S
authors: Lv
authors: Tingyun
authors: T
authors: Kuang
authors: Yinxin
authors: Y
authors: Li
mesh_terms: D015153:Blotting, Western; D057486:Carbon Cycle / Q000187:drug effects*; D019663:Chenopodiaceae / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008954:Models, Biological; D009584:Nitrogen / Q000378:metabolism*; D010788:Photosynthesis; D020543:Proteome / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology*; D055051:Salt-Tolerant Plants / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry; D060431:Thylakoid Membrane Proteins / Q000378:metabolism
chemical_list: D060365:Chloroplast Proteins; D020543:Proteome; D060431:Thylakoid Membrane Proteins; D012965:Sodium Chloride; D009584:Nitrogen