title: Angiogenic factors as diagnostic tests for preeclampsia: a performance comparison between two commercial immunoassays.
abstract: OBJECTIVE
  Placental growth factor and soluble Fms-like tyrosine kinase-1 may be potential diagnostic markers of preeclampsia. We compared performances of 2 immunoassays, the Triage placental growth factor assay and the Elecsys soluble Fms-like tyrosine kinase-1/placental growth factor ratio in diagnosing preeclampsia.
  
  
  METHODS
  A single site, case-control study of 44 patients with preeclampsia and 84 matched normal pregnant controls. Samples were collected at the time of diagnosis. Assays were performed according to product inserts.
  
  
  RESULTS
  Both assays had optimal performance in diagnosing early-onset preeclampsia with area under the receiver operating characteristic curves of 0.99 (Triage: 100% sensitivity, 96% specificity; Elecsys: 64% sensitivity, 100% specificity for early-onset preeclampsia). Reassignment of the Elecsys cutoff for a positive test based on receiver operating characteristic curves increased sensitivity to 92%.
  
  
  CONCLUSIONS
  Using product insert cutoffs, Triage appears to have greater sensitivity at only a small reduction in specificity compared with Elecsys in the diagnosis of early-onset preeclampsia. A different cutoff may improve Elecsys sensitivity.
authors: Department of Obstetrics and Gynaecology, Faulty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Samantha J
authors: SJ
authors: Benton
authors: Yuxiang
authors: Y
authors: Hu
authors: Fang
authors: F
authors: Xie
authors: Kenneth
authors: K
authors: Kupfer
authors: Seok-Won
authors: SW
authors: Lee
authors: Laura A
authors: LA
authors: Magee
authors: Peter
authors: P
authors: von Dadelszen
mesh_terms: D000328:Adult; D043925:Angiogenesis Inducing Agents / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007118:Immunoassay; D007159:Immunologic Tests; D000072483:Placenta Growth Factor; D011225:Pre-Eclampsia / Q000097:blood / Q000175:diagnosis*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood*
chemical_list: D043925:Angiogenesis Inducing Agents; D015415:Biomarkers; C000608036:PGF protein, human; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D040281:Vascular Endothelial Growth Factor Receptor-1