title: Co-culture of melanocytes with adipose-derived stem cells as a potential substitute for co-culture with keratinocytes.
abstract: Cell-to-cell interactions between melanocytes and keratinocytes increase the proliferation and migration of melanocytes. In fact, mixed keratinocyte and melanocyte cultures have been used for autologous cell transplantation for treatment of vitiligo. However, this may require taking an amount of skin tissue large enough to leave scars. In this study, the in vitro effect of adipose-derived stem cells (ADSCs) on proliferation, differentiation and migration of melanocytes was compared with that of keratinocytes using immunohistochemistry and a Boyden chamber migration assay. The proliferation and migration of melanocytes was significantly stimulated by co-culture with ADSCs compared with melanocyte monocultures, al-though the effect of ADSCs was less powerful than that of keratinocytes. This may be related to increases in stem cell factor and basic fibroblast growth factor, growth factors for melanocytes, produced by the ADSCs. The ratios of melanocytes stained with antibodies against Trp-2, E-cadherin and N-cadherin were significantly increased by co-culturing with ADSCs compared with co-culturing with keratinocytes as well as melanocyte monocultures. The proportion of less-pigmented melanocytes was also increased and sustained for a longer duration in the presence of ADSCs. Our data show that co-culturing with ADSCs results in increased melanocyte proliferation and migration while reducing differentiation, and could provide a means to treat disorders such as vitiligo.
authors: Department of Dermatology, Dongguk University Ilsan Hospital, Gyeonggi-do, South Korea.
authors: Ji-Young
authors: JY
authors: Kim
authors: Chan Do
authors: CD
authors: Park
authors: Joon Ho
authors: JH
authors: Lee
authors: Chang-Hoon
authors: CH
authors: Lee
authors: Byung-Rok
authors: BR
authors: Do
authors: Ai Young
authors: AY
authors: Lee
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000704:Analysis of Variance; D015820:Cadherins / Q000378:metabolism; D002450:Cell Communication; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation; D019089:Stem Cell Factor / Q000378:metabolism; D013234:Stem Cells / Q000378:metabolism
chemical_list: D015820:Cadherins; D019089:Stem Cell Factor; D016222:Fibroblast Growth Factor 2; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase