title: Abdominal hernia repair with a decellularized dermal scaffold seeded with autologous bone marrow-derived mesenchymal stem cells.
abstract: Surgeons usually use synthetic polymer meshes for abdominal wall hernia repair. However, synthetic polymer meshes exhibit a lack of growth and related complications. In this study, we produced a tissue-engineered patch for abdominal hernia repair. Autologous bone-marrow-derived mesenchymal stem cells (BMSCs) were isolated and proliferated in vitro; decellularized dermal scaffolds (DSs) were prepared using enzymatic process; and then BMSCs were seeded onto the DSs for the construction of tissue-engineered patches. Under general anesthesia, rabbits underwent creation of abdominal wall defects and which were repaired with BMSC-seeded DSs, acellular DSs, and skin sutures only, respectively. Animals were sacrificed after 2 months for assessing the histological and gross examination. Abdominal hernias were absent in animals repaired with cell-seeded group, and abdominal hernias or bulges appeared in all animals repaired with acellular group. All the animals that were not repaired died within 10 days. The cell-seeded implants were thicker and indicated good angiogenesis compared with that of the acellular implants, both in histological and gross examination. The tissue-engineered patches prepared with BMSCs seeding on DSs can be used for abdominal wall hernia repair.
authors: Department of Vascular Surgery, Zhongshan Hospital, Xiamen University Department of Emergency, Zhongshan Hospital, Xiamen University, 201 Hubinnan Road, Xiamen, China.
authors: Yilin
authors: Y
authors: Zhao
authors: Zhigang
authors: Z
authors: Zhang
authors: Jinling
authors: J
authors: Wang
authors: Ping
authors: P
authors: Yin
authors: Jianyin
authors: J
authors: Zhou
authors: Maochuan
authors: M
authors: Zhen
authors: Wugeng
authors: W
authors: Cui
authors: Gang
authors: G
authors: Xu
authors: Donghai
authors: D
authors: Yang
authors: Zhongchen
authors: Z
authors: Liu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology*; D046449:Hernia, Abdominal / Q000473:pathology / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010537:Peritoneum / Q000166:cytology / Q000473:pathology; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*