title: Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography.
abstract: Comprehensive glycoprotein characterization based on mass spectrometry (MS) is challenging because of low concentration of glycopeptides and suppression effect of abundant non-glycosylated peptides in MS. Therefore, it is vital to enrich glycopeptides before MS analysis. A new method was developed to selectively enrich glycopeptides from complex sample by coupling C18 fractionation with titanium dioxide (TiO(2)) enrichment. The new method allows to selectively enrich N-linked glycopeptides with various glycan forms and different sequence lengths. Compared with single TiO(2) method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. Further application of this method to mixture of non-glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples.
authors: Department of General Surgery, Affiliated Union Hospital of Huazhong University of Science and Technology, Wuhan, Hubei Province, P. R. China.
authors: Bo
authors: B
authors: Zhang
authors: Qianying
authors: Q
authors: Sheng
authors: Xiuling
authors: X
authors: Li
authors: Qi
authors: Q
authors: Liang
authors: Jingyu
authors: J
authors: Yan
authors: Xinmiao
authors: X
authors: Liang
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D013058:Mass Spectrometry; D010449:Peptide Mapping; D014025:Titanium / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; C009495:titanium dioxide; D014025:Titanium