title: Composites of aminodextran-coated Fe3O4 nanoparticles and graphene oxide for cellular magnetic resonance imaging.
abstract: Formation of composites of dextran-coated Fe(3)O(4) nanoparticles (NPs) and graphene oxide (Fe(3)O(4)-GO) and their application as T(2)-weighted contrast agent for efficient cellular magnetic resonance imaging (MRI) are reported. Aminodextran (AMD) was first synthesized by coupling reaction of carboxymethyldextran with butanediamine, which was then chemically conjugated to meso-2,3-dimercaptosuccinnic acid-modified Fe(3)O(4) NPs. Next, the AMD-coated Fe(3)O(4) NPs were anchored onto GO sheets via formation of amide bond in the presence of 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC). It is found that the Fe(3)O(4)-GO composites possess good physiological stability and low cytotoxicity. Prussian Blue staining analysis indicates that the Fe(3)O(4)-GO nanocomposites can be internalized efficiently by HeLa cells, depending on the concentration of the composites incubated with the cells. Furthermore, compared with the isolated Fe(3)O(4) NPs, the Fe(3)O(4)-GO composites show significantly enhanced cellular MRI, being capable of detecting cells at the iron concentration of 5 μg mL(-1) with cell density of 2 × 10(5) cells mL(-1), and at the iron concentration of 20 μg mL(-1) with cell density of 1000 cells mL(-1).
authors: Division of Nanobiomedicine, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou 215123, China.
authors: Weihong
authors: W
authors: Chen
authors: Peiwei
authors: P
authors: Yi
authors: Yi
authors: Y
authors: Zhang
authors: Liming
authors: L
authors: Zhang
authors: Zongwu
authors: Z
authors: Deng
authors: Zhijun
authors: Z
authors: Zhang
mesh_terms: D002477:Cells / Q000000981:diagnostic imaging*; D003287:Contrast Media / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006108:Graphite; D006367:HeLa Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D011859:Radiography
chemical_list: D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D010087:Oxides; D006108:Graphite