title: Multilineage priming of enhancer repertoires precedes commitment to the B and myeloid cell lineages in hematopoietic progenitors.
abstract: Recent studies have documented genome-wide binding patterns of transcriptional regulators and their associated epigenetic marks in hematopoietic cell lineages. In order to determine how epigenetic marks are established and maintained during developmental progression, we have generated long-term cultures of hematopoietic progenitors by enforcing the expression of the E-protein antagonist Id2. Hematopoietic progenitors that express Id2 are multipotent and readily differentiate upon withdrawal of Id2 expression into committed B lineage cells, thus indicating a causative role for E2A (Tcf3) in promoting the B cell fate. Genome-wide analyses revealed that a substantial fraction of lymphoid and myeloid enhancers are premarked by the poised or active enhancer mark H3K4me1 in multipotent progenitors. Thus, in hematopoietic progenitors, multilineage priming of enhancer elements precedes commitment to the lymphoid or myeloid cell lineages.
authors: Department of Molecular Biology, University of California, San Diego, La Jolla, CA 92093, USA.
authors: Elinore M
authors: EM
authors: Mercer
authors: Yin C
authors: YC
authors: Lin
authors: Christopher
authors: C
authors: Benner
authors: Suchit
authors: S
authors: Jhunjhunwala
authors: Janusz
authors: J
authors: Dutkowski
authors: Martha
authors: M
authors: Flores
authors: Mikael
authors: M
authors: Sigvardsson
authors: Trey
authors: T
authors: Ideker
authors: Christopher K
authors: CK
authors: Glass
authors: Cornelis
authors: C
authors: Murre
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D022423:Myeloid Cells / Q000166:cytology*; D040081:Protein Array Analysis
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; C407909:TCF3 protein, human