title: Electrospun nanofiber-based regeneration of cartilage enhanced by mesenchymal stem cells.
abstract: Application of biomaterials in combination with stem cells is a novel tissue engineering approach to regenerate cartilage. The objective of this study was to investigate the potential of poly(vinyl alcohol)/polycaprolactone (PVA/PCL) nanofiber scaffolds seeded with rabbit bone marrow-mesenchymal stem cell (BM-MSC) for cartilage tissue engineering in vitro and in vivo. We tested the biocompatibility and mechanical properties of nanofibrous scaffolds using scanning electron microscope, MTT assay, and tensile measurements. The capacity of MSC for chondrogenic differentiation on scaffolds was examined using reverse transcription-polymer chain reaction and immunostaining. For in vivo assessments, PVA/PCL nanofiber scaffolds with or without MSC were implanted into rabbit full-thickness cartilage defects. To evaluate cartilage regeneration, semi-quantitative grading and histological analysis were performed. Our results showed that PVA/PCL scaffolds supported the proliferation and chondrogenic differentiation of MSC in vitro. Moreover, the animals treated with cell-seeded PVA/PCL scaffolds showed improved healing of defects compared with untreated control and those which received cell-free scaffolds. Our findings suggest that PVA/PCL scaffolds incorporated with MSC can serve as a suitable graft for articular cartilage reconstruction.
authors: Stem Cell Biology, Nanotechnology and Tissue Engineering Departments, Stem Cell Technology Research Center, Tehran, Iran.
authors: Abbas
authors: A
authors: Shafiee
authors: Masoud
authors: M
authors: Soleimani
authors: Gholamreza Abedi
authors: GA
authors: Chamheidari
authors: Ehsan
authors: E
authors: Seyedjafari
authors: Masumeh
authors: M
authors: Dodel
authors: Amir
authors: A
authors: Atashi
authors: Yousof
authors: Y
authors: Gheisari
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D011091:Polyesters / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D013718:Tensile Strength / Q000187:drug effects; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D011091:Polyesters; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C016240:polycaprolactone; D011142:Polyvinyl Alcohol