title: Quantitative analysis of eletriptan in human plasma by HPLC-MS/MS and its application to pharmacokinetic study.
abstract: Authors developed a simple, sensitive, selective, rapid, rugged, and reproducible liquid chromatography-tandem mass spectrometry method for the quantification of eletriptan (EP) in human plasma using naratriptan (NP) as an internal standard (IS). Chromatographic separation was performed on Ascentis Express C18, 50 × 4.6 mm, 2.7 μm column. Mobile phase was composed of 0.1% formic acid: methanol (40:60 v/v), with 0.5 mL/min flow rate. Drug and IS were extracted by liquid-liquid extraction. EP and NP were detected with proton adducts at m/z 383.2→84.3 and 336.2→97.8 in multiple reaction monitoring (MRM) positive mode, respectively. The method was validated with the correlation coefficients of (r(2)) ≥ 0.9963 over a linear concentration range of 0.5-250.0 ng/mL. This method demonstrated intra- and inter-day precision within 1.4-9.2% and 4.4-5.5% and accuracy within 96.8-103% and 98.5-99.8% for EP. This method is successfully applied in the bioequivalence study of 24 human volunteers.
authors: Chalapathi Institute of Pharmaceutical Sciences, Lam, Guntur, Andhra Pradesh 522034, India.
authors: Venkata Suresh
authors: VS
authors: Ponnuru
authors: B R
authors: BR
authors: Challa
authors: Ramarao
authors: R
authors: Nadendla
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D059625:Liquid-Liquid Extraction / Q000379:methods; D010880:Piperidines / Q000097:blood; D011759:Pyrrolidines / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014363:Tryptamines / Q000097:blood*
chemical_list: D010880:Piperidines; D011759:Pyrrolidines; D017366:Serotonin Receptor Agonists; D014363:Tryptamines; C115647:eletriptan; C106783:naratriptan