title: Salivary peptidome in type 1 diabetes mellitus.
abstract: Diabetic patients show a high susceptibility to oral diseases of inflammatory, catabolic and chronic nature with potential impact on saliva composition. In this study, our purpose was to characterize type 1 diabetes-induced alterations in the salivary peptidome aiming to find prospective biomarkers for type 1 diabetes oral health evaluation. Peptidomic analysis of saliva from controls (n = 5) and type 1 diabetic patients (n = 5) were performed by liquid chromatography followed by mass spectrometry. The proteolytic activity and metalloproteinases expression was accessed by zymography and slot blot analysis, respectively. Data evidenced a significant increase in the percentage of peptides in diabetic patients paralleled by a higher proteolytic activity, compared with healthy individuals. The nonsalivary gland protein fragments identified in saliva were mainly derived from collagen and extracellular matrix proteins, namely collagen type I. The cleavage site frequency analysis showed significant differences between healthy and type 1 diabetic individuals, highlighting the activity of proteases such as matrix metalloproteinase-9 and cathepsin D. Our results highlight salivary collagen fragments as potential biomarkers to follow up diabetes-related oral damage.
authors: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
authors: Armando
authors: A
authors: Caseiro
authors: Rui
authors: R
authors: Vitorino
authors: António S
authors: AS
authors: Barros
authors: Rita
authors: R
authors: Ferreira
authors: Maria João
authors: MJ
authors: Calheiros-Lobo
authors: Davide
authors: D
authors: Carvalho
authors: José Alberto
authors: JA
authors: Duarte
authors: Francisco
authors: F
authors: Amado
mesh_terms: D000293:Adolescent; D000328:Adult; D020816:Amino Acid Motifs; D016022:Case-Control Studies; D020134:Catalytic Domain; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D006801:Humans; D016018:Least-Squares Analysis; D008297:Male; D010455:Peptides / Q000032:analysis*; D059748:Proteolysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012463:Saliva / Q000737:chemistry* / Q000201:enzymology
chemical_list: D010455:Peptides; D020543:Proteome