title: An ATP-grasp ligase involved in the last biosynthetic step of the iminomycosporine shinorine in Nostoc punctiforme ATCC 29133.
abstract: We investigated the genetic basis for mycosporine sunscreen biosynthesis by the cyanobacterium Nostoc punctiforme ATCC 29133. Heterologous expression in Escherichia coli of three contiguous N. punctiforme genes (NpR5600, NpR5599, and NpR5598, here named mysA, mysB, and mysC, respectively) led to the production of mycosporine-glycine, an oxomycosporine. Additional expression of gene NpF5597 (mysD) led to the conversion of mycosporine-glycine into iminomycosporines (preferentially shinorine but also others like mycosporine-2-glycine and porphyra-334). This represents a new mode of enzymatic synthesis for iminomycosporines, one that differs in genetic origin, mechanism, and apparent substrate specificity from that known in Anabaena variabilis ATCC 29413. These results add to the emerging profile of the protein family of ATP-dependent ligases, to which the mysC product belongs, as important condensation enzymes in microbial secondary metabolism.
authors: School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.
authors: Qunjie
authors: Q
authors: Gao
authors: Ferran
authors: F
authors: Garcia-Pichel
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D046870:Anabaena variabilis / Q000235:genetics; D053898:Biosynthetic Pathways / Q000235:genetics*; D003514:Cyclohexylamines; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D023061:Gene Order; D005998:Glycine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D046937:Nostoc / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics; D026801:Synteny
chemical_list: D003514:Cyclohexylamines; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; C494891:shinorine; D008025:Ligases; D005998:Glycine