title: [Application of enriched bone marrow compound with fibrin glue in repairing old radial bone defect in rabbits].
abstract: OBJECTIVE
  To explore the feasibility of using enriched bone marrow (BM) compound with fibrin glue (FG) in repairing old radial bone defect.
  
  
  METHODS
  Totally 36 New Zealand rabbits were equally randomized into three groups: simple FG group, BM+FG group, and enriched BM+FG group. A 1.5-cm segmental bone defect was made at the left radial in each animal. After one month, the defect was implanted with the engineered bone. Before implantation, a compound of enriched BM with FG underwent electron microscopy, long-term culture, and bacteriological culture. Four, 8, and 12 weeks after operations, the osteogenetic effect was evaluated using X-ray observation, HE staining, or Van Gieson staining, and a semi-quantitative analysis was performed.
  
  
  RESULTS
  Electron microscopy showed enriched BM were compatible well with FG. No bacterial contamination or oncogenicity was observed after long-term culture. X-ray showed the repair effectiveness was significantly higher in BM+FG group and enriched BM+FG group than in simple FG group. Eight and 12 weeks after surgery, the Yang scores were significantly higher in enriched BM+FG group than in BM+FG group [(9.348±0.364évs.(7.984±0.229éìF=40.167ìP=0.001; (12.664±0.388)vs. (10.584±0.836é, F=20.3647ìP=0.004]. In addition, the Yang's scores at bone defects in BM+FG group and enriched BM+FG group were higher at the 12(th) week than in the 8(th) week. (F=36.004ìP=0.001; F=155.141ìP=0.000; respectively)The bone defects were repaired at varied degrees were histologically observed in BM+FG group and enriched BM+FG group during the observations.
  
  
  CONCLUSIONS
  Implantation of BM+FG or enriched BM+FG are effective in repairing old radial bone defects, while simple FG shows not such effect.
authors: Department of Orthopaedics, First Hospital of Hangzhou, Hangzhou, China. yaowx04@yahoo.com.cn
authors: Wang-xiang
authors: WX
authors: Yao
authors: An
authors: A
authors: Ma
authors: Liu-long
authors: LL
authors: Zhu
authors: Kai
authors: K
authors: Lu
authors: Min
authors: M
authors: Zhu
authors: Zhao-xiang
authors: ZX
authors: Peng
authors: Zhen-yu
authors: ZY
authors: Bian
authors: Qi-fang
authors: QF
authors: He
mesh_terms: D000818:Animals; D001853:Bone Marrow; D018786:Bone Substitutes; D004195:Disease Models, Animal; D005260:Female; D015718:Fibrin Tissue Adhesive; D008297:Male; D011817:Rabbits; D011884:Radius / Q000293:injuries* / Q000601:surgery; D023822:Tissue Engineering
chemical_list: D018786:Bone Substitutes; D015718:Fibrin Tissue Adhesive