title: Lobocrassins A-E: new cembrane-type diterpenoids from the soft coral Lobophytum crassum.
abstract: Five new cembrane-type diterpenoids, lobocrassins A-E (1-5), were isolated from the soft coral Lobophytum crassum. The structures of cembranes 1-5 were established by spectroscopic and chemical methods and by comparison of the spectral data with those of known cembrane analogues. Lobocrassin A (1) is the first cembranoid possessing an α-chloromethyl-α-hydroxy-γ-lactone functionality and is the first chlorinated cembranoid from soft corals belonging to the genus Lobophytum. Lobocrassins B (2) and C (3) were found to be the stereoisomers of the known cembranes, 14-deoxycrassin (6) and pseudoplexaurol (7), respectively. Lobocrassin B (2) exhibited modest cytotoxicity toward K562, CCRF-CEM, Molt4, and HepG2 tumor cells and displayed significant inhibitory effects on the generation of superoxide anion and the release of elastase by human neutrophils.
authors: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
authors: Chia-Ying
authors: CY
authors: Kao
authors: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.|Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors: Jui-Hsin
authors: JH
authors: Su
authors: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.
authors: Mei-Chin
authors: MC
authors: Lu
authors: Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan.
authors: Tsong-Long
authors: 0000-0002-5780-3977
authors: TL
authors: Hwang
authors: National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.|Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors: Wei-Hsien
authors: WH
authors: Wang
authors: Department of Pharmacy, Tajen University, Pingtung 907, Taiwan.
authors: Jih-Jung
authors: JJ
authors: Chen
authors: Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors: Jyh-Horng
authors: JH
authors: Sheu
authors: Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung 404, Taiwan.
authors: Yueh-Hsiung
authors: 0000-0001-5935-6755
authors: YH
authors: Kuo
authors: Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors: Ching-Feng
authors: CF
authors: Weng
authors: Department of Sport, Health, and Leisure, Cheng Shiu University, Kaohsiung 833, Taiwan.
authors: Lee-Shing
authors: LS
authors: Fang
authors: Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors: Zhi-Hong
authors: 0000-0003-4411-044X
authors: ZH
authors: Wen
authors: Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung 944, Taiwan.|National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan.|Division of Marine Biotechnology, Asia-Pacific Ocean Research Center, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804, Taiwan.|Department of Life Science and Institute of Biotechnology, National Dong Hwa University, Hualien 974, Taiwan.
authors: Ping-Jyun
authors: 0000-0002-6644-9236
authors: PJ
authors: Sung
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D020014:K562 Cells; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013237:Stereoisomerism; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D013481:Superoxides; C079901:14-deoxycrassin; C079902:pseudoplexaurol; C037071:cembrane; D010196:Pancreatic Elastase