title: Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers.
abstract: Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T rubrum (CBS 120358), T mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.
authors: Department of Genetics of Microorganisms, University of Łódź, Poland. anidobro@biol.uni.lodz.pl
authors: Anita
authors: A
authors: Dobrowolska
authors: Joanna
authors: J
authors: Debska
authors: Magdalena
authors: M
authors: Kozłowska
authors: Paweł
authors: P
authors: Staczek
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D003883:Arthrodermataceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D008864:Microsporum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008875:Middle Aged; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D011044:Poland; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D014005:Tinea / Q000382:microbiology*; D014006:Tinea Capitis / Q000382:microbiology; D055815:Young Adult
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal