title: Fluorescence detection of coralyne and polyadenylation reaction using an oligonucleotide-based fluorogenic probe.
abstract: This study describes the development of a simple, sensitive, and selective-detection system for coralyne and polyadenylation reaction based on the fact that coralyne induces a conformational change of the polyadenosine [poly(A)] oligonucleotide through A-coralyne-A coordination, thereby enhancing the fluorescence of SYBR Green I.
authors: Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
authors: Yen-Hsiu
authors: YH
authors: Lin
authors: Wei-Lung
authors: WL
authors: Tseng
mesh_terms: D052160:Benzothiazoles; D001600:Berberine Alkaloids / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D003959:Diamines; D005456:Fluorescent Dyes / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D009930:Organic Chemicals / Q000737:chemistry; D026723:Polyadenylation; D011804:Quinolines; D012091:Repetitive Sequences, Nucleic Acid; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D052160:Benzothiazoles; D001600:Berberine Alkaloids; D003959:Diamines; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; C000666:coralyne