title: Detection of rolling circle amplified DNA molecules using probe-tagged magnetic nanobeads in a portable AC susceptometer.
abstract: Here, the volume-amplified magnetic nanobead detection assay (VAM-NDA) is for the first time applied for detection of rolling circle amplified (RCA) DNA molecules in a portable, commercial AC susceptometer that operates at ambient temperatures and with an analysis time of about 20 min. The performance of the assay is investigated using three different magnetic nanobead sizes: 50, 130 and 250nm. The performance of the assay using the AC susceptometer is compared to the performance achieved using a superconducting quantum interference device (SQUID). It is found that the performance of the assay is comparable in the two setups with a quantitative detection limit of ∼4pM for all bead sizes under study. The findings show that the VAM-NDA holds promise for future wide-spread implementation in commercial AC susceptometer setups thus opening up for the possibility to perform magnetic bead-based DNA detection in point-of-care and outpatient settings.
authors: Department of Engineering Sciences, Division of Nanotechnology and Functional Materials, Uppsala University, The Ångström Laboratory, Box 534, SE-751 21 Uppsala, Sweden.
authors: Teresa Zardán Gómez
authors: TZ
authors: de la Torre
authors: Anja
authors: A
authors: Mezger
authors: David
authors: D
authors: Herthnek
authors: Christer
authors: C
authors: Johansson
authors: Peter
authors: P
authors: Svedlindh
authors: Mats
authors: M
authors: Nilsson
authors: Maria
authors: M
authors: Strømme
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004270:DNA, Circular / Q000032:analysis* / Q000235:genetics; D006801:Humans; D058185:Magnetite Nanoparticles; D021141:Nucleic Acid Amplification Techniques; D015345:Oligonucleotide Probes / Q000235:genetics; D010316:Particle Size
chemical_list: D004270:DNA, Circular; D058185:Magnetite Nanoparticles; D015345:Oligonucleotide Probes