title: Comparison of loop-mediated isothermal amplification and real-time PCR for the diagnosis of tuberculous pleurisy.
abstract: OBJECTIVE
  Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy.
  
  
  RESULTS
  Investigation of pleural effusion samples from patients with tuberculous pleurisy, majority of them smear-/culture-negative, and control individuals with non-TB diseases showed that the LAMP assay with incubation time of 60 min has much higher specificity and the LAMP assay with incubation time of 90 min has significantly higher sensitivity in the diagnosis of tuberculous pleurisy, as compared with fluorescent real-time PCR.
  
  
  CONCLUSIONS
  The MTBC-LAMP is a useful assay for the diagnosis of tuberculous pleurisy, especially in pleural effusion smear-/culture-negative patients.
  
  
  CONCLUSIONS
  Tuberculous pleural effusion usually contains low number of mycobacteria, which leads to low diagnostic sensitivity of acid-fast staining and mycobacterial culture methods. In this study, we developed a simple and sensitive LAMP assay for the diagnosis of tuberculous pleurisy. This assay should have broad application in resource-limited settings.
authors: Division of Research, Institute of Tuberculosis, 309 Hospital, Beijing, China.
authors: B
authors: B
authors: Yang
authors: X
authors: X
authors: Wang
authors: H
authors: H
authors: Li
authors: G
authors: G
authors: Li
authors: Z
authors: Z
authors: Cao
authors: X
authors: X
authors: Cheng
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D001681:Biological Assay; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014396:Tuberculosis, Pleural / Q000175:diagnosis* / Q000382:microbiology