title: Application of analytical electron microscopic methods to investigate the function of spherites in the midgut of the larval antlion Euroleon nostras (Neuroptera: Myrmeleontidae).
abstract: This study presents an application of analytical electron microscopy in biology to investigate the chemical composition of the spherites and to elucidate the importance of these methods in the life sciences. The structure of the spherites in the midgut cells of first, second, and third instar larvae Euroleon nostras was investigated by a combination of transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDXS), electron energy-loss spectroscopy (EELS), and energy filtering TEM (EFTEM). The structure and chemical composition of the spherites changed during the metamorphosis. In first larvae, the spherites are composed of amorphous, flocculent material, containing C, N, and O. In second larvae and third ones, the spherites have concentric layers of alternating electron-dense and electron-lucent material. In second larvae, Si, P, Ca, and Fe are accumulated in the spherite organic matrix, composed of C, N, and O. In the spherites of third larvae, additionally Al was found. Therefore, the spherites are thought to store organic compounds in all three larval stages of E. nostras and additionally inorganic compounds in second and third ones. In first larvae, spherites are present in the midgut cells; in second and third larvae, they are present in the cells of the midgut and in its lumen. It could be suggested that the spherites might be involved in the regulation of the appropriate mineral composition of the internal environment and could serve as the accumulation site of nontoxic waste materials that cannot be metabolized.
authors: Medical Faculty, University of Maribor, Slomškov trg 15, Maribor SI-2000, Slovenia. sasa.lipovsek@uni-mb.si
authors: Saška
authors: S
authors: Lipovšek
authors: Ilse
authors: I
authors: Letofsky-Papst
authors: Ferdinand
authors: F
authors: Hofer
authors: Maria Anna
authors: MA
authors: Pabst
authors: Dušan
authors: D
authors: Devetak
mesh_terms: D000818:Animals; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000648:ultrastructure; D007313:Insecta / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D007814:Larva / Q000737:chemistry / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission / Q000379:methods; D013052:Spectrometry, X-Ray Emission