title: Cytotoxicity of water-soluble fraction from biodiesel and its diesel blends to human cell lines.
abstract: The designation of biodiesel as a green fuel has increased its commercialization and use, making its fate in the environment a matter of concern. Fuel spills constitute a major source of aquatic pollution and, like diesel spills, biodiesel can produce adverse effects on aquatic environments, animals and humans. The present study assessed cytotoxic effects of water systems contaminated with neat biodiesel and its diesel blends by means of different procedures on human T cell leukemia (Jurkat) and human hepatocellular carcinoma (HepG2) cells [detection of changes in mitochondrial membrane potential (ΔΨ(m)) using tetramethylrhodamine ethyl ester (TMRE), apoptosis recognition by Annexin V and impedance real-time cell analyzer (xCELLigence™ system)]. The data obtained showed concordance across the different bioassays, with cytotoxic effects observed as a dose-dependent response only for waters contaminated with pure diesel (D100) and B5 blend, which is characterized by a mixture of 95% diesel and 5% biodiesel. The data can also lead us to hypothesize that diesel accounts for the harmful effects observed, and that biodiesel does not worsen the impacts caused by diesel pollution.
authors: Biology Department, Univ Estadual Paulista (UNESP), Av. 24A, 1515, Bela Vista, Rio Claro, SP 13506-900, Brazil.
authors: Daniela Morais
authors: DM
authors: Leme
authors: Tamara
authors: T
authors: Grummt
authors: Rita
authors: R
authors: Heinze
authors: Andrea
authors: A
authors: Sehr
authors: Matthias
authors: M
authors: Skerswetat
authors: Mary Rosa Rodrigues
authors: MR
authors: de Marchi
authors: Marcos Canto
authors: MC
authors: Machado
authors: Danielle Palma
authors: DP
authors: de Oliveira
authors: Maria Aparecida
authors: MA
authors: Marin-Morales
mesh_terms: D056804:Biofuels / Q000633:toxicity*; D002460:Cell Line; D003603:Cytotoxins / Q000737:chemistry / Q000633:toxicity*; D004787:Environmental Pollution; D005742:Gasoline / Q000633:toxicity*; D006801:Humans; D012995:Solubility; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D056804:Biofuels; D003603:Cytotoxins; D005742:Gasoline; D014874:Water Pollutants, Chemical