title: Composition and antioxidant activity of water-soluble polysaccharides from Tuber indicum.
abstract: Crude water-soluble Chinese truffle Tuber indicum polysaccharide (TIP) was extracted from the fruiting bodies with water and then successively purified by DEAE-cellulose 52 and Sephadex G-100 column chromatography, yielding two major polysaccharide fractions: TIP1-1 and TIP2-1. High-performance gel permeation chromatography analysis showed that the average molecular sizes of TIP1-1 and TIP2-1 were approximately 1.75×10(4) Da and 5.73×10(3) Da, respectively. Monosaccharide component analysis by gas chromatography indicated that TIP1-1 was composed of mannose, glucose, galactose, and rhamannose in the respective molar ratio of 3.93:1.24:0.75:1.26 and that TIP2-1 contained mannose, glucose, and arabinose in the respective molar ratio of 5.27:1.44:0.43. The antioxidant activity analyses revealed that TIP1-1 and TIP2-1 possessed considerable antioxidant activity. Compared with TIP1-1, which has a higher molecular weight and contains no uronic acid, TIP2-1 exhibited a protective effect on PC12 cells injured by H(2)O(2) and a higher scavenging activity against free radicals. The relative effects of the lower molecular size, the presence of uronic acid, and the antioxidant activity of TIP2-1 appear to be significant. Accordingly, the Chinese truffle T. indicum might serve as an effective antioxidative healthcare food and source of natural antioxidants.
authors: Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
authors: Qiang
authors: Q
authors: Luo
authors: Jie
authors: J
authors: Zhang
authors: Liang
authors: L
authors: Yan
authors: Yuanlin
authors: Y
authors: Tang
authors: Xiang
authors: X
authors: Ding
authors: Zhirong
authors: Z
authors: Yang
authors: Qun
authors: Q
authors: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001089:Arabinose / Q000032:analysis; D001203:Ascomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D003911:Dextrans; D005690:Galactose / Q000032:analysis; D006861:Hydrogen Peroxide / Q000633:toxicity; D008358:Mannose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010851:Picrates / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D003911:Dextrans; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; C025614:sephadex; D001089:Arabinose; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D008358:Mannose; D005690:Galactose