title: The potential of mouse skin-derived precursors to differentiate into             mesenchymal and neural lineages and their application to osteogenic induction             in vivo.
abstract: Although previous studies indicate that skin-derived precursors (SKPs) are multipotent dermal precursors that share similarities with neural crest stem cells (NCSCs), a shared ability for multilineage differentiation toward neural crest lineages between SKPs and NCSCs has not been fully demonstrated. Here, we report the derivation of SKPs from adult mouse skin and their directed multilineage differentiation toward neural crest lineages. Under controlled in vitro conditions, mouse SKPs were propagated and directed toward peripheral nervous system lineages such as peripheral neurons and Schwann cells, and mesenchymal lineages, such as osteogenic, chondrogenic, adipogenic, and smooth muscle cells. To ask if SKPs could generate these same lineages in vivo, a mixture of SKP-derived mesenchymal stem cells and hydroxyapatite/tricalcium phosphate was transplanted into the rat calvarial defects. Over the ensuing 4 weeks, we observed formation of osteogenic structure in the calvarial defect without any evidence of teratomas. These findings demonstrate the multipotency of adult mouse SKPs to differentiate into neural crest lineages. In addition, SKP-derived mesenchymal stem cells represent an accessible, potentially autologous source of precursor cells for tissue-engineered bone repair.
authors: Department of Oral Biochemistry and Program in Cancer and Developmental                     Biology, Seoul National University School of Dentistry, Seoul 110-749,                     Republic of Korea.
authors: Hyun Ki
authors: HK
authors: Kang
authors: Seung-Ki
authors: SK
authors: Min
authors: Sung Youn
authors: SY
authors: Jung
authors: Kyoungsuk
authors: K
authors: Jung
authors: Da Hyun
authors: DH
authors: Jang
authors: O Bok
authors: OB
authors: Kim
authors: Gae-Sig
authors: GS
authors: Chun
authors: Zang Hee
authors: ZH
authors: Lee
authors: Byung-Moo
authors: BM
authors: Min
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology; D005260:Female; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology; D009432:Neural Crest / Q000166:cytology; D009474:Neurons / Q000166:cytology / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000502:physiology; D012867:Skin / Q000166:cytology*; D012886:Skull / Q000166:cytology* / Q000293:injuries; D033581:Stem Cell Transplantation / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*