title: Lymphocyte subpopulations in Chinese women with Turner syndrome.
abstract: OBJECTIVE
  Turner syndrome (TS) is associated with deficiency of cellular and humoral immunity. However, the characteristics of lymphocyte subpopulations in Chinese women with TS have not been reported. In this study, the percentage of lymphocyte subpopulations and the mRNA expression of some transcription factors were determined in patients with TS. The effect of the hormone substitution on lymphocyte subpopulations was also analyzed.
  
  
  METHODS
  Thirteen Chinese TS women and eight age and sex-matched healthy volunteers were studied. The percentage and mean fluorescence intensity (MFI) of lymphocyte subpopulations including CD3+CD4+, CD3+CD8+, CD19-CD138+, CD4+CD25+FoxP3+ and CD4+CD8-IL17A+ cells were determined by flow cytometry. The mRNA expression of some transcription factors were detected by RT-PCR.
  
  
  RESULTS
  Compared to control, the percentage of CD3+CD4+ cells was significantly reduced (p < 0.05), while the percentage of CD19-CD138+, CD4+CD25+FoxP3+ and CD4+CD8-IL17A+ cells was significantly increased in TS patients. No difference was observed in the percentage of CD3+CD8+, CD19+ B cells between TS patients and healthy volunteers, with the similar changes in the mean fluorescence intensity of these cells. The mRNA expression of some transcription factors slightly enhanced in TS patients. Estrogen therapy did not affect the percentage of lymphocyte subpopulations.
  
  
  CONCLUSIONS
  These findings suggested that Turner syndrome might be associated with changes of lymphocyte subpopulations.
authors: Immunology and Reproductive Biology Lab of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China.
authors: Hongye
authors: H
authors: Fan
authors: Dandan
authors: D
authors: Wang
authors: Haiyan
authors: H
authors: Zhu
authors: Jie
authors: J
authors: Li
authors: Yali
authors: Y
authors: Hu
authors: Yayi
authors: Y
authors: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D044466:Asian People; D004967:Estrogens / Q000627:therapeutic use; D005260:Female; D006801:Humans; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D014157:Transcription Factors / Q000378:metabolism; D014424:Turner Syndrome / Q000188:drug therapy / Q000276:immunology*; D055815:Young Adult
chemical_list: D004967:Estrogens; D014157:Transcription Factors