title: Enhancing antigen cross-presentation and T-cell priming by complexing protein antigen to recombinant large heat-shock protein.
abstract: Large heat-shock proteins (HSPs), including hsp110 and grp170, are unique immunochaperones capable of carrying and introducing antigens into professional antigen-presenting cells for efficient cross-presentation. Therefore, reconstituted chaperone complexes of large HSPs and protein antigen may be exploited for augmentation of an antigen-specific immune response. The methods for the preparation of the recombinant protein antigen chaperone complex and characterization of its T-cell priming capability in both in vitro and in vivo settings are described.
authors: Department of Human and Molecular Genetics, Massey Cancer Center, Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, VA, USA. xywang@vcu.edu
authors: Xiang-Yang
authors: XY
authors: Wang
authors: Huanfa
authors: H
authors: Yi
authors: Xiaofei
authors: X
authors: Yu
authors: Damin
authors: D
authors: Zuo
authors: John R
authors: JR
authors: Subjeck
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D019496:Cancer Vaccines / Q000276:immunology; D045142:Cross-Priming / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism; D050957:HSP110 Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D018832:Molecular Chaperones / Q000276:immunology; D017510:Protein Folding; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D006023:Glycoproteins; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D018832:Molecular Chaperones; D011994:Recombinant Proteins; C098410:glucose-regulated protein 170