title: Effects of poly(amidoamine) dendrimers on ocular absorption of puerarin using microdialysis.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effects of poly(amidoamine) (PAMAM) dendrimers on ocular absorption of puerarin.
  
  
  METHODS
  The samples of rabbits' aqueous humor were collected by in vivo microdialysis. The ocular pharmacokinetic properties of puerarin were measured to evaluate the effects of PAMAM dendrimers on ocular absorption of puerarin.
  
  
  RESULTS
  In the pharmacokinetic studies, the AUC(0∼∞) values of puerarin solution with 0.2% (w/v) PAMAM dendrimers (G3.0, G4.0, G5.0) were greater than those in the control group by 2.3-, 3.5-, and 2.1-folds, respectively. The C(max) values of puerarin solution with 0.2% (w/v) PAMAM dendrimers were 1.5, 2.5, and 1.3 times the values of the control group and the t(1/2) values were significantly longer than that of control group.
  
  
  CONCLUSIONS
  Using PAMAM dendrimers might be a promising strategy to enhance the absorption of puerarin. The generation of PAMAM dendrimers may play a key role in increasing the ocular absorption of puerarin.
authors: School of Pharmacy, Yantai University, Yantai, P.R. China.
authors: Chen
authors: C
authors: Yao
authors: Wenyan
authors: W
authors: Wang
authors: Xiudi
authors: X
authors: Zhou
authors: Tao
authors: T
authors: Qu
authors: Hongjie
authors: H
authors: Mu
authors: Rongcai
authors: R
authors: Liang
authors: Aiping
authors: A
authors: Wang
authors: Kaoxiang
authors: K
authors: Sun
mesh_terms: D000042:Absorption; D060433:Administration, Ophthalmic; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D019540:Area Under Curve; D001682:Biological Availability; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D007529:Isoflavones / Q000008:administration & dosage / Q000493:pharmacokinetics*; D017551:Microdialysis; D011817:Rabbits; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*
chemical_list: D050091:Dendrimers; D004337:Drug Carriers; D007529:Isoflavones; C104700:PAMAM Starburst; D014665:Vasodilator Agents; C033607:puerarin