title: Dietary plasma proteins attenuate the innate immunity response in a mouse model of acute lung injury.
abstract: We examined whether oral plasma protein supplements affect the innate immune response in a model of acute lung inflammation. Mice were fed diets supplemented with 8 % spray-dried plasma (SDP) or 2 % plasma Ig concentrate (IC) from day 19 (weaning) until day 34. The mice were challenged with intranasal lipopolysaccharide (LPS) at day 33 (and killed 24 h later for cytokine and leucocyte analyses) or at day 34 (and killed 6 h later for cytokine determinations). In bronchoalveolar lavage fluid (BALF), LPS increased the number of leucocytes by twenty-sevenfold, an effect that was partly prevented by both SDP and IC, and by twentyfold the percentage of activated monocytes, which was partly prevented by SDP. In the lung tissue, LPS increased the infiltrated leucocytes, and this effect was prevented in part by SDP. In unchallenged mice, both SDP and IC diets reduced the percentage of resident neutrophils and monocytes (P < 0·05). In the blood, both SDP and IC completely prevented LPS-dependent monocyte activation (CD14⁺; P < 0·05). LPS dramatically increased the concentration of cytokines (TNF-α, IL-1α, IL-6, granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor) and chemokines (CXCL1, CCL2, CCL3 and CCL4) in BALF. The acute response of cytokine production was reduced by 20-80 % by both SDP and IC. For chemokines, plasma supplements had no effect on LPS-induced CXCL1 expression but significantly reduced CCL2, CCL3 and CCL4 production (P < 0·05). The results support the view that dietary plasma proteins can be used to attenuate endotoxin-associated lung inflammation.
authors: Group of Physiology and Experimental Nutrition, Department of Physiology, Faculty of Pharmacy, Nutrition and Food Safety Research Institute, University of Barcelona, Avinguda de Joan XXIII s/n, E-08028, Barcelona, Spain.
authors: Mònica
authors: M
authors: Maijó
authors: Lluïsa
authors: L
authors: Miró
authors: Javier
authors: J
authors: Polo
authors: Joy
authors: J
authors: Campbell
authors: Louis
authors: L
authors: Russell
authors: Joe
authors: J
authors: Crenshaw
authors: Eric
authors: E
authors: Weaver
authors: Miquel
authors: M
authors: Moretó
authors: Anna
authors: A
authors: Pérez-Bosque
mesh_terms: D055371:Acute Lung Injury / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology; D000818:Animals; D001798:Blood Proteins / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002417:Cattle; D002452:Cell Count; D016207:Cytokines / Q000032:analysis; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D011014:Pneumonia / Q000209:etiology / Q000517:prevention & control*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D034421:Sus scrofa
chemical_list: D001798:Blood Proteins; D016207:Cytokines; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse