title: Global proteomic assessment of the classical protein-tyrosine phosphatome and "Redoxome".
abstract: Protein-tyrosine phosphatases (PTPs), along with protein-tyrosine kinases, play key roles in cellular signaling. All Class I PTPs contain an essential active site cysteinyl residue, which executes a nucleophilic attack on substrate phosphotyrosyl residues. The high reactivity of the catalytic cysteine also predisposes PTPs to oxidation by reactive oxygen species, such as H(2)O(2). Reversible PTP oxidation is emerging as an important cellular regulatory mechanism and might contribute to diseases such as cancer. We exploited these unique features of PTP enzymology to develop proteomic methods, broadly applicable to cell and tissue samples, that enable the comprehensive identification and quantification of expressed classical PTPs (PTPome) and the oxidized subset of the PTPome (oxPTPome). We find that mouse and human cells and tissues, including cancer cells, display distinctive PTPomes and oxPTPomes, revealing additional levels of complexity in the regulation of protein-tyrosine phosphorylation in normal and malignant cells.
authors: Department of Medical Biophysics, University of Toronto, Toronto M5G 2M9, ON, Canada. rkarisch@uhnresearch.ca
authors: Robert
authors: R
authors: Karisch
authors: Minerva
authors: M
authors: Fernandez
authors: Paul
authors: P
authors: Taylor
authors: Carl
authors: C
authors: Virtanen
authors: Jonathan R
authors: JR
authors: St-Germain
authors: Lily L
authors: LL
authors: Jin
authors: Isaac S
authors: IS
authors: Harris
authors: Jun
authors: J
authors: Mori
authors: Tak W
authors: TW
authors: Mak
authors: Yotis A
authors: YA
authors: Senis
authors: Arne
authors: A
authors: Östman
authors: Michael F
authors: MF
authors: Moran
authors: Benjamin G
authors: BG
authors: Neel
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000378:metabolism; D010084:Oxidation-Reduction; D017027:Protein Tyrosine Phosphatases / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D051381:Rats
chemical_list: D017027:Protein Tyrosine Phosphatases