title: The relationship between chromosomal positioning within the nucleus and the SSD1 gene in Saccharomyces cerevisiae.
abstract: Eukaryotic cells are characterized by very large chromosomal DNAs efficiently packed within the nucleus. To identify the mechanism of chromosomal packaging based on the uniqueness of the centromere region in Saccharomyces cerevisiae, we isolated the HCH6 mutant, which shows 2.5-fold higher efficiency of site-specific recombination between the CEN5 and HIS3 loci than the wild-type CH53 strain. This mutant also displayed defects in cell integrity at high temperature. The SSD1 gene was perhaps responsible for this defect. The efficiency of site-specific recombination was decreased by the introduction of SSD1 in HCH6 cells and increased by disruption of SSD1 in the wild-type cells. Furthermore, the distances between the CEN5 and HIS3 loci and between the CEN5 locus and the spindle pole body (SPB) indicated that disrupting SSD1 caused a loss of the anchoring of the CEN5 locus near SPB. These results suggest Ssd1p-dependent cross-talk between chromosomal positioning within the nucleus and the positioning of cellular components within the cell.
authors: Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University.
authors: Toshiaki
authors: T
authors: Yanamoto
authors: Akihiro
authors: A
authors: Miyamoto
authors: Kayoko
authors: K
authors: Ikeda
authors: Takushi
authors: T
authors: Hatano
authors: Hiroaki
authors: H
authors: Matsuzaki
mesh_terms: D000483:Alleles; D002467:Cell Nucleus / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics; D056426:Genetic Loci; D016681:Genome, Fungal; D006358:Hot Temperature; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D008941:Spindle Apparatus / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C485219:Ssd1 protein, S cerevisiae