title: Preparation and evaluation of molecularly imprinted microspheres for solid-phase extraction of 1,4-hydroxybenzoic acid esters in soy.
abstract: Molecularly imprinted microspheres (MIMs) were prepared by precipitation polymerization for the binding and recognition of 1,4-hydroxybenzoic acid esters. Ethyl p-hydroxybenzoate (EtPHB) was used as the template molecule, methacrylic acid as the functional monomer, ethylene dimethacrylate as the linking agent. It was evaluated by solid-phase extraction column packed with MIMs combined with liquid chromatography to determine trace preservatives including benzoic acid, methyl p-hydroxybenzoate, EtPHB, propyl p-hydroxybenzoate in food products. A solid-phase extraction based on MIM procedure was used to isolate four additives from the food matrix before quantitative analysis. The Scatchard plot suggested that the template-polymer system had two-site binding behavior with the dissociation constants of 0.3577 and 3.952 mg/g, respectively. The rebinding test, based on the molecularly imprinted solid-phase extraction column technique, showed the recoveries of soy samples spiked with four additives within 88.4-110.6%, with the relative standard deviations of 1.97-3.82%. Finally, the method was successfully applied for the analysis of parabens in foodstuff without traditional pretreatment.
authors: School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, P. R. China. juanhe@haut.edu.cn
authors: Juan
authors: J
authors: He
authors: Yanzheng
authors: Y
authors: Shen
authors: Si
authors: S
authors: Chen
authors: Hongliang
authors: H
authors: Wei
authors: Jing
authors: J
authors: Zhu
authors: Liqin
authors: L
authors: You
authors: Kui
authors: K
authors: Lu
mesh_terms: D004952:Esters / Q000032:analysis / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D008863:Microspheres; D054802:Molecular Imprinting; D010226:Parabens / Q000032:analysis / Q000302:isolation & purification*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D004952:Esters; D010226:Parabens; D011108:Polymers; C038193:4-hydroxybenzoic acid