title: The elevated serum S100A8/A9 during acute myocardial infarction is not of cardiac myocyte origin.
abstract: Overproduction of circulating S100A8/A9 occurs in patients following acute myocardial infarction (AMI). It remains unclear whether ischemia insult per se induces S100A8 and S100A9 expression in cardiac myocytes or even whether the cardiac myocytes participate as a source of these proteins. In this study, western blot analysis and quantitative real-time reverse transcription polymerase chain reaction were used to test samples obtained from isolated spontaneously hypertensive rat hearts and Wistar-Kyoto rat hearts subjected to global normothermic ischemia and from neonatal Wistar rat cardiac myocytes undergoing hypoxia. Ischemia did not increase the expression of S100A8 and S100A9 proteins and mRNA in the myocardium either from the spontaneously hypertensive rat hearts or the Wistar-Kyoto rat hearts. In addition, the levels of S100A8 and S100A9 proteins were unchanged in the neonatal rat cardiac myocytes undergoing hypoxia. However, both ischemia and hypoxia activated NF-kappaB in ischemic myocardium and in hypoxic cardiac cells in a time-dependent manner. The results suggest that the increased serum S100A8/A9 concentrations following AMI were not of cardiac myocyte origin.
authors: Institute of Cardiology, the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qing Chun Road, Hangzhou, Zhejiang 310003, People's Republic of China.
authors: Chang-Qing
authors: CQ
authors: Du
authors: Lin
authors: L
authors: Yang
authors: Jie
authors: J
authors: Han
authors: Jian
authors: J
authors: Yang
authors: Xue-Yan
authors: XY
authors: Yao
authors: Xiao-Sheng
authors: XS
authors: Hu
authors: Shen-Jiang
authors: SJ
authors: Hu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure; D040501:Calgranulin A / Q000097:blood* / Q000235:genetics; D040502:Calgranulin B / Q000097:blood* / Q000235:genetics; D015687:Cell Hypoxia; D002478:Cells, Cultured; D008297:Male; D009203:Myocardial Infarction / Q000097:blood*; D017202:Myocardial Ischemia / Q000097:blood* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017208:Rats, Wistar
chemical_list: D040501:Calgranulin A; D040502:Calgranulin B; D016328:NF-kappa B; D012333:RNA, Messenger