title: A novel anti-inflammatory role for ginkgolide B in asthma via inhibition of the ERK/MAPK signaling pathway.
abstract: Ginkgolide B is an anti-inflammatory extract of Ginkgo biloba and has been used therapeutically. It is a known inhibitor of platelet activating factor (PAF), which is important in the pathogenesis of asthma. Here, a non-infectious mouse model of asthma is used to evaluate the anti-inflammatory capacity of ginkgolide B (GKB) and characterize the interaction of GKB with the mitogen activated protein kinase (MAPK) pathway. BALB/c mice that were sensitized and challenged to ovalbumin (OVA) were treated with GKB (40 mg/kg) one hour before they were challenged with OVA. Our study demonstrated that GKB may effectively inhibit the increase of T-helper 2 cytokines, such as interleukin (IL)-5 and IL-13 in bronchoalveolar lavage fluid (BALF). Furthermore, the eosinophil count in BALF significantly decreased after treatment of GKB when compared with the OVA-challenged group. Histological studies demonstrated that GKB substantially inhibited OVA-induced eosinophilia in lung tissue and mucus hyper-secretion by goblet cells in the airway. These results suggest that ginkgolide B may be useful for the treatment of asthma and its efficacy is related to suppression of extracellular regulating kinase/MAPK pathway.
authors: Key Laboratory of Zoonosis Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors: Xiao
authors: X
authors: Chu
authors: Xinxin
authors: X
authors: Ci
authors: Jiakang
authors: J
authors: He
authors: Miaomiao
authors: M
authors: Wei
authors: Xiaofeng
authors: X
authors: Yang
authors: Qingjun
authors: Q
authors: Cao
authors: Hongyu
authors: H
authors: Li
authors: Shuang
authors: S
authors: Guan
authors: Yanhong
authors: Y
authors: Deng
authors: Daxin
authors: D
authors: Pang
authors: Xuming
authors: X
authors: Deng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000473:pathology; D005260:Female; D046934:Ginkgolides / Q000494:pharmacology* / Q000627:therapeutic use; D020397:Goblet Cells / Q000473:pathology; D006965:Hyperplasia; D007073:Immunoglobulin E / Q000097:blood; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D007783:Lactones / Q000494:pharmacology* / Q000627:therapeutic use; D008168:Lung / Q000378:metabolism / Q000473:pathology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008264:Macrophages / Q000473:pathology; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009504:Neutrophils / Q000473:pathology; D010047:Ovalbumin
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D046934:Ginkgolides; D018793:Interleukin-13; D015848:Interleukin-5; D007783:Lactones; D007073:Immunoglobulin E; D010047:Ovalbumin; C045856:ginkgolide B; D020928:Mitogen-Activated Protein Kinases