title: Mycobacterium indicus pranii is a potent immunomodulator for a recombinant vaccine against human chorionic gonadotropin.
abstract: The objective of this work was to identify a human use-permissible adjuvant to enhance significantly the antibody response to a recombinant anti-hCG vaccine. Previous Phase II efficacy trials in sexually active women have demonstrated the prevention of pregnancy at hCG bioneutralization titers of 50ng/ml or more. Mycobacterium indicus pranii (MIP), a non-pathogenic Mycobacterium employed as an autoclaved suspension in aqueous buffer, significantly increased antibody titers in the FVB strain of mice. Three other genetic strains of mice: SJL, C3H, and C57Bl/6 responded with antibody titers several-fold higher than 50 ng/ml, which is the protective threshold in women, although there were differences in the peak titers attained. In addition, the duration of the antibody response was lengthened. The vaccine hCGβ-LTB, given together with MIP, induces both a Th1 and Th2 response, which is reflected in the production of not only IgG1, but also a high proportion of IgG2a and IgG2b antibodies.
authors: Talwar Research Foundation, E-8 Neb valley, Neb Sarai, New Delhi-110 068, India.
authors: Shilpi
authors: S
authors: Purswani
authors: G P
authors: GP
authors: Talwar
authors: Richa
authors: R
authors: Vohra
authors: Rahul
authors: R
authors: Pal
authors: Amulya K
authors: AK
authors: Panda
authors: Nirmal K
authors: NK
authors: Lohiya
authors: Jagdish C
authors: JC
authors: Gupta
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D006063:Chorionic Gonadotropin / Q000276:immunology* / Q000494:pharmacology; D017322:Clinical Trials, Phase II as Topic; D003271:Contraceptive Agents, Female / Q000276:immunology* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007155:Immunologic Factors / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009161:Mycobacterium / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology / Q000494:pharmacology
chemical_list: D000906:Antibodies; D006063:Chorionic Gonadotropin; D003271:Contraceptive Agents, Female; D007155:Immunologic Factors; D014614:Vaccines, Synthetic