title: Altered intestinal epithelium-associated lymphocyte repertoires and             function in ApcMin/+ mice.
abstract: ApcMin/+ mice spontaneously develop multiple intestinal adenomas along the             length of the small intestine and colon. Currently little is known about the role             of the immune system in regulating intestinal tumorigenesis in these animals.             This study characterised small intestinal intraepithelial lympho-- cyte (IEL)             populations in C56BL/6J ApcMin/+ mice and wild-type (Apc+/+) mice. We also determined             the effect that T cells expressing either γδ or αβ encoded T cell receptors (TcR)             exert on intestinal tumorigenesis. ApcMin/+ mice had significantly lower numbers             of CD3+ IELs compared with Apc+/+ littermates and displayed reduced cytotoxicity             against tumour target cells. Further analysis of IEL cytotoxicity revealed differences             in the cytotoxic pathways utilised by IELs in ApcMin/+ and Apc+/+ mice with ApcMin/+             IELs displaying an absence of perforin/granzyme-mediated killing and increased             levels of Fas-FasL-mediated cytotoxicity compared with wild-type IELs. Analysis             of ApcMin/+ mice crossed with αβ T-cell deficient (TcRβ-/-) or γδ T-cell deficient             (TcRδ-/-) mice on the same genetic background revealed decreased tumour multiplicity             in the absence of both αβ and γδ T-cells. This study demonstrates that altered             T-cell subsets play important roles in promoting tumorigenesis in ApcMin/+ mice             and forms the basis for future mechanistic studies.
authors: Institute of Molecular and Cellular Biology, University of Leeds,                     Leeds LS2 9JT, UK.
authors: Lorraine
authors: L
authors: Marsh
authors: P Louise
authors: PL
authors: Coletta
authors: Mark A
authors: MA
authors: Hull
authors: Peter J
authors: PJ
authors: Selby
authors: Simon R
authors: SR
authors: Carding
mesh_terms: D000236:Adenoma / Q000276:immunology; D025601:Adenomatous Polyposis Coli Protein / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D005260:Female; D007413:Intestinal Mucosa / Q000276:immunology*; D007414:Intestinal Neoplasms / Q000276:immunology*; D007421:Intestine, Small / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta