title: Inhibition of cell motility and invasion by HangAmDan-B in NCI-H460             human non-small cell lung cancer cells.
abstract: Correlation between inhibition of cell motility and anti-invasive activity             by the water extract of HangAmDan-B (HAD-B), a crude extract of eight Korean medicinal             animals and plants, in NCI-H460 human non-small cell lung cancer (NSCLC) cells             was investigated. Within the concentrations that were not cytotoxic, HAD-B induced             significant concentration-dependent inhibition of cell motility and invasiveness             of NCI-H460 cells. Treatment with HAD-B resulted in dose-dependent inhibition             of the activities of matrix metallo-proteinase (MMP)-2 and MMP-9, and this was             correlated with a decrease in expression of their mRNA and proteins, and upregulation             of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 expression. Anti-invasive             activity of HAD-B was also found to be associated with increased tightness of             the tight junction (TJ), as demonstrated by an increase in transepithelial electrical             resistance. In addition, the present results indicated that treatment with HAD-B             resulted in repression of the levels of claudin family members, which are major             components of TJs that play a key role in control and selectivity of para-cellular             transport. Although further studies are needed, findings from the present study             indicate that TJs and MMPs are critical targets of HAD-B-induced anti-invasiveness             in NCI-H460 NSCLC cells.
authors: East-West Cancer Center, Dunsan Oriental Medical Hospital of Daejeon University, Daejeon 301-724, Republic of Korea.
authors: Yong Jun
authors: YJ
authors: Choi
authors: Dong Yeok
authors: DY
authors: Shin
authors: Yeon-Weol
authors: YW
authors: Lee
authors: Chong Kwan
authors: CK
authors: Cho
authors: Gi-Young
authors: GY
authors: Kim
authors: Wun-Jae
authors: WJ
authors: Kim
authors: Hwa Seung
authors: HS
authors: Yoo
authors: Yung Hyun
authors: YH
authors: Choi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002289:Carcinoma, Non-Small-Cell Lung; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D057167:Claudins / Q000235:genetics / Q000378:metabolism; D045424:Complex Mixtures / Q000494:pharmacology*; D057075:Enzyme Assays; D015870:Gene Expression; D006801:Humans; D008175:Lung Neoplasms; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D019108:Tight Junctions / Q000378:metabolism; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D057167:Claudins; D045424:Complex Mixtures; D061965:Matrix Metalloproteinase Inhibitors; C560822:TIMP1 protein, human; C560823:TIMP2 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020782:Matrix Metalloproteinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9