title: Biodegradation of endosulfan by Mortieralla sp. strain W8 in soil: Influence of different substrates on biodegradation.
abstract: To examine the bioremediation potential of Mortierella sp. strain W8 in endosulfan contaminated soil, the fungus was inoculated into sterilized and unsterilized soil spiked with endosulfan. Wheat bran and cane molasses were used as substrates to understand the influence of different organic materials on the degradation of endosulfan in soil. Strain W8 degraded α- and β-endosulfan in both sterilized and unsterilized soil. In unsterilized soil with wheat bran+W8, α- and β- endosulfan were degraded by approximately 80% and 50%, respectively after 28 d incubation against the initial endosulfan concentration (3 mg kg(-1) dw). The corresponding values for α- and β-endosulfan degradation with wheat bran only were 50% and 3%. Endosulfan diol metabolite was detected after 14 d incubation in wheat bran+W8 whereas it was not found with wheat bran only. Production of endosulfan sulfate, the main metabolite of endosulfan, was suppressed with wheat bran+W8 treatment compared with wheat bran only. It was demonstrated that wheat bran is a more suitable substrate for strain W8 than cane molasses. Wheat bran+W8 is a superior fungus and substrate mix for bioremediation in soil contaminated with endosulfan.
authors: Organochemicals Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors: Ryota
authors: R
authors: Kataoka
authors: Kazuhiro
authors: K
authors: Takagi
authors: Futa
authors: F
authors: Sakakibara
mesh_terms: D001673:Biodegradation, Environmental; D002849:Chromatography, Gas; D004043:Dietary Fiber / Q000382:microbiology; D004726:Endosulfan / Q000032:analysis / Q000378:metabolism*; D013058:Mass Spectrometry; D008965:Molasses / Q000382:microbiology; D020094:Mortierella / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004043:Dietary Fiber; D012989:Soil Pollutants; D004726:Endosulfan