title: A novel sialic acid binding lectin with anti-bacterial activity from the Hong Kong oyster (Crassostrea hongkongensis).
abstract: Lectins play an important role in immune recognition and host defense. In the present study, a full-length cDNA encoding a novel sialic acid binding lectin was cloned from Crassostrea hongkongensis (designated Ch-salectin) by rapid amplification of cDNA ends (RACE). It is 531 bp in length, containing a 21 bp 5' UTR, a 39 bp 3' UTR and a 468 bp ORF coding for 156 amino acids. The Ch-salectin protein contains a signal peptide and a conserved complement component C1q domain. The purified recombinant MBP-tagged Ch-salectin protein can bind to a sialic acid containing protein fetuin and significantly inhibit the growth of both Gram-negative and Gram-positive bacteria. Furthermore, the transcription of Ch-salectin was inducible and significantly up-regulated during Vibrio alginolyticus infection. Thus, these results highlight the essential roles of Ch-salectin in immune recognition and host defense against bacterial infection in C. hongkongensis.
authors: Key Laboratory of Marine Bio-resources Sustainable Utilization, Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
authors: Xiaocui
authors: X
authors: He
authors: Yang
authors: Y
authors: Zhang
authors: Feng
authors: F
authors: Yu
authors: Ziniu
authors: Z
authors: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D049896:Crassostrea / Q000235:genetics* / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D060748:Fetuins / Q000378:metabolism; D040461:Gene Components; D005786:Gene Expression Regulation / Q000276:immunology*; D037102:Lectins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012394:Rosaniline Dyes; D017422:Sequence Analysis, DNA / Q000662:veterinary; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D044149:Vibrio alginolyticus / Q000276:immunology*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D060748:Fetuins; D037102:Lectins; D012394:Rosaniline Dyes; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C048139:Coomassie blue