title: Correlative 3D imaging of whole mammalian cells with light and electron microscopy.
abstract: We report methodological advances that extend the current capabilities of ion-abrasion scanning electron microscopy (IA-SEM), also known as focused ion beam scanning electron microscopy, a newly emerging technology for high resolution imaging of large biological specimens in 3D. We establish protocols that enable the routine generation of 3D image stacks of entire plastic-embedded mammalian cells by IA-SEM at resolutions of ∼10-20nm at high contrast and with minimal artifacts from the focused ion beam. We build on these advances by describing a detailed approach for carrying out correlative live confocal microscopy and IA-SEM on the same cells. Finally, we demonstrate that by combining correlative imaging with newly developed tools for automated image processing, small 100nm-sized entities such as HIV-1 or gold beads can be localized in SEM image stacks of whole mammalian cells. We anticipate that these methods will add to the arsenal of tools available for investigating mechanisms underlying host-pathogen interactions, and more generally, the 3D subcellular architecture of mammalian cells and tissues.
authors: Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
authors: Gavin E
authors: GE
authors: Murphy
authors: Kedar
authors: K
authors: Narayan
authors: Bradley C
authors: BC
authors: Lowekamp
authors: Lisa M
authors: LM
authors: Hartnell
authors: Jurgen A W
authors: JA
authors: Heymann
authors: Jing
authors: J
authors: Fu
authors: Sriram
authors: S
authors: Subramaniam
mesh_terms: D000818:Animals; D002477:Cells / Q000648:ultrastructure*; D006046:Gold / Q000737:chemistry; D015497:HIV-1 / Q000648:ultrastructure*; D054884:Host-Pathogen Interactions; D006801:Humans; D021621:Imaging, Three-Dimensional; D018613:Microscopy, Confocal / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*
chemical_list: D006046:Gold