title: Submicroparticles composed of amphiphilic chitosan derivative for oral insulin and curcumin release applications.
abstract: Amphiphilic polymers for dual drug delivery have been a focus of research in recent years. We have previously developed and characterized Lauroyl sulphated chitosan (LSCS). Here biological characterizations like mucoadhesion, cytotoxicity, calcium binding, tight junction opening and enzymatic degradation studies were performed to understand its applicability. In vitro drug release properties of both hydrophilic insulin and hydrophobic curcumin were carried out. The biological activity and stability of released insulin were also studied. The stability studies of encapsulated curcumin and uptake studies have also been carried out. LSCS showed strong mucoadhesion and 100% of non-toxicity. LSCS could transiently open tight junctions between Caco-2 cells and thus increase the paracellular permeability. LSCS enhanced calcium binding properties and decreased enzymatic degradation rate retaining insulin activity. LSCS could protect curcumin from photodegradation and could also enter into the cells. From release studies, LSCS was found to be a suitable candidate for both drugs.
authors: Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Thiruvananthapuram 695012, India. sharmacp@sctimst.ac.in
authors: R
authors: R
authors: Shelma
authors: Chandra P
authors: CP
authors: Sharma
mesh_terms: D000284:Administration, Oral; D018938:Caco-2 Cells; D048271:Chitosan / Q000737:chemistry*; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D004337:Drug Carriers / Q000009:adverse effects / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007328:Insulin / Q000008:administration & dosage* / Q000737:chemistry; D013501:Surface-Active Agents / Q000009:adverse effects / Q000737:chemistry; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D007328:Insulin; D013501:Surface-Active Agents; D048271:Chitosan; D003474:Curcumin