title: Rapid automated detection of ABL kinase domain mutations in imatinib-resistant patients.
abstract: ABL tyrosine kinase inhibitor (TKI), imatinib is used for BCR-ABL(+) leukemias. We developed an automatic method utilizing guanine-quenching probes (QP) to detect 17 kinds of mutations frequently observed in imatinib-resistance. Results were obtained from 100μL of whole blood within 90min by this method. Detected mutations were almost identical between QP method and direct sequencing. Furthermore, the mutation-biased PCR (MBP) was added to the QP method to increase sensitivity, resulting earlier detection of T315I mutation which was insensitive to any ABL TKIs. Thus, the QP and MBP-QP may become useful methods for the management of ABL TKI-treated patients.
authors: Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, Japan.
authors: Ruriko
authors: R
authors: Tanaka
authors: Shinya
authors: S
authors: Kimura
authors: Eishi
authors: E
authors: Ashihara
authors: Mariko
authors: M
authors: Yoshimura
authors: Naoto
authors: N
authors: Takahashi
authors: Hisashi
authors: H
authors: Wakita
authors: Kuniaki
authors: K
authors: Itoh
authors: Kaichi
authors: K
authors: Nishiwaki
authors: Kenshi
authors: K
authors: Suzuki
authors: Rina
authors: R
authors: Nagao
authors: Hisayuki
authors: H
authors: Yao
authors: Yoshihiro
authors: Y
authors: Hayashi
authors: Sakiko
authors: S
authors: Satake
authors: Hideyo
authors: H
authors: Hirai
authors: Ken-Ichi
authors: K
authors: Sawada
authors: Oliver G
authors: OG
authors: Ottmann
authors: Junia V
authors: JV
authors: Melo
authors: Taira
authors: T
authors: Maekawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001331:Automation; D001549:Benzamides; D002648:Child; D002675:Child, Preschool; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D000068877:Imatinib Mesylate; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010879:Piperazines / Q000627:therapeutic use*; D016133:Polymerase Chain Reaction; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics*; D011743:Pyrimidines / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D016315:Proto-Oncogene Proteins c-abl