title: Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA.
abstract: Serum antibody titers are a useful measurement of protection against infection (feline panleukopenia virus [FPV]) or clinical disease (feline herpesvirus-1 [FHV] and feline calicivirus [FCV]), and their determination has been recommended as part of disease outbreak management in animal shelters. The objective of this study was to determine the sensitivity, specificity, and inter-observer and inter-assay agreement of two semi-quantitative point-of-care assays for the detection of protective antibody titers (PAT) against FPV, FHV and FCV in shelter cats. Low sensitivity for FPV antibodies (28%) rendered a canine point-of-care assay inappropriate for use in cats. The feline point-of-care assay also had low sensitivity (49%) and low negative predictive value (74%) for FPV PAT detection, but was highly accurate in the assessment of FHV and FCV PAT. Improvements in accuracy and repeatability of FPV PAT determination could make this tool a valuable component of a disease outbreak response in animal shelters.
authors: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.
authors: Brian A
authors: BA
authors: Digangi
authors: Lauren K
authors: LK
authors: Gray
authors: Julie K
authors: JK
authors: Levy
authors: Edward J
authors: EJ
authors: Dubovi
authors: Sylvia J
authors: SJ
authors: Tucker
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary; D017927:Calicivirus, Feline / Q000276:immunology; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005254:Feline Panleukopenia / Q000175:diagnosis; D028301:Feline Panleukopenia Virus / Q000276:immunology; D006564:Herpesviridae / Q000276:immunology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary; D019095:Point-of-Care Systems; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014777:Virus Diseases / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral