title: Study of the adjuvanticity of lysine lipopeptides; carbamate analogs elicit strong Th1 and Th2 response to ovalbumin in mice.
abstract: Bacterial lipoproteins and their synthetic analogs are strong immune modulators of the early host responses. In view of the strong adjuvanticity of bacterial lipopeptide mimics bearing lysine residues, a focused library of lipidated dipeptides and tripeptides has been synthesized with a view to understand the pattern of activity vis a vis the site and extent of lipidation. Compounds 4, 5 and 14 stimulate OVA specific IgG titer, neutralization of antibodies (IgG1 and IgG2a), T lymphocyte sub-sets (CD4/CD8) and its production of soluble mediators for Th1 (IFN-γ)/Th2 (IL-4) cytokines and costimulatory molecules (CD80/CD86) which are ideal traits of immune adjuvants. The results support lipidated lysine dipeptides as potent enhancers of humoral and cell mediated immune responses and thus might become promising immune-adjuvants for self adjuvanted vaccines.
authors: Synthetic and Biological Chemistry Division, Indian Institute of Integrative Medicine, Jammu 180001, India.
authors: Tabasum
authors: T
authors: Ismail
authors: Syed
authors: S
authors: Shafi
authors: Amit
authors: A
authors: Gupta
authors: Anamika
authors: A
authors: Khajuria
authors: Tabasum
authors: T
authors: Sidiq
authors: Doma Mahendhar
authors: DM
authors: Reddy
authors: M Sarwar
authors: MS
authors: Alam
authors: G N
authors: GN
authors: Qazi
authors: H M Sampath
authors: HM
authors: Kumar
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D015703:Antigens, CD / Q000097:blood; D002219:Carbamates / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000097:blood; D016130:Immunophenotyping; D055666:Lipopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D008239:Lysine / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D010047:Ovalbumin / Q000276:immunology; D060327:Solid-Phase Synthesis Techniques; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects* / Q000276:immunology; D014611:Vaccination / Q000379:methods
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D015703:Antigens, CD; D002219:Carbamates; D016207:Cytokines; D007074:Immunoglobulin G; D055666:Lipopeptides; D010047:Ovalbumin; D008239:Lysine