title: Hyaluronic acid as a rescue therapy for trinitrobenzene sulfonic acid-induced colitis through Cox-2 and PGE2 in a Toll-like receptor 4-dependent way.
abstract: We hypothesized whether systemic administration of high-molecular-weight hyaluronic acid (HMW HA) could rescue trinitrobenzene sulfonic acid (TNBS)-induced colitis through Toll-like receptor 4 (TLR4) signal. C3H/HeN mice and C3H/HeJ mice were used. Mice were divided into four groups: control, 50% ethanol treatment group, TNBS treatment group, and TNBS plus HA treatment group. The weight changes, clinical scores, macroscopic scores, and histological scores were recorded. Cyclooxygenase 2 (Cox-2) and prostaglandin E(2) (PGE(2)) expressions were measured both in colons and peritoneal macrophages from these mice. HA was a rescue therapy for the colitis induced by TNBS only in C3H/HeN mice. The clinical score, macroscopic score, and histological score were much lower in C3H/HeN mice receiving TNBS plus HA treatment. Cox-2 and PGE(2) expressions only increased in C3H/HeN mice. These Cox-2 expressing cells were macrophages. HA can also promote the production of Cox-2 and PGE(2) in peritoneal macrophages from C3H/HeN mice. Our data demonstrated that HMW HA can rescue TNBS-induced colitis through inducing Cox-2 and PGE(2) expressions in a TLR4-dependent way. Macrophages may be the effector cells of HMW HA.
authors: Department of Gastroenterology, West China Hospital of Sichuan University, Chengdu, China.
authors: Huan
authors: H
authors: Chen
authors: Mahesh
authors: M
authors: Mahaseth
authors: Yan
authors: Y
authors: Zhang
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D007150:Immunohistochemistry / Q000379:methods; D017737:Macrophages, Peritoneal / Q000166:cytology; D051379:Mice; D008809:Mice, Inbred C3H; D008856:Microscopy, Fluorescence / Q000379:methods; D051197:Toll-Like Receptor 4 / Q000378:metabolism*; D051193:Toll-Like Receptors / Q000378:metabolism; D016896:Treatment Outcome; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
chemical_list: D051197:Toll-Like Receptor 4; D051193:Toll-Like Receptors; D014302:Trinitrobenzenesulfonic Acid; D006820:Hyaluronic Acid; D051546:Cyclooxygenase 2; D015232:Dinoprostone