title: Enantioseparation of organic acids of pharmaceutical interest using eremomycin as a chiral selector.
abstract: Strong adsorption of eremomycin on the fused-silica capillary wall was used for separation of enantiomers by CE. The capillary with adsorbed chiral selector was shown to be easily prepared and has reproducible properties. The effect of the chiral selector concentration, pH and composition of the BGE, and applied voltage on enantioseparation of acidic compounds, such as profens and aromatic carboxylic acids, was investigated. Two native α-amino acids, aspartic acid and glutamic acid, were enantioseparated. Fourteen tested compounds (including amino acids) were baseline resolved. Good selectivity of separation (α>1.09) was achieved. The migration order of ibuprofen and ketoprofen enantiomers was determined. The procedures were proposed for the analysis of flurbiprofen and warfarin in pharmaceuticals. Linearity was achieved in the concentration range of 4.0×10(-5)-2.0×10(-3) M for flurbiprofen and 3.2×10(-6)-4.9×10(-6) M for warfarin. The detection limits were found to be about 1×10(-5) M for flurbiprofen and 1×10(-6) M for warfarin.
authors: Division of Analytical Chemistry, Chemistry Department, Lomonosov Moscow State University, Leninskie Gory, Moscow, Russia. alexapro@gmail.com
authors: Aleksandra F
authors: AF
authors: Prokhorova
authors: Vladimir A
authors: VA
authors: Larin
authors: Anna N
authors: AN
authors: Mikhalyuk
authors: Sergei M
authors: SM
authors: Staroverov
authors: Elena N
authors: EN
authors: Shapovalova
authors: Oleg A
authors: OA
authors: Shpigun
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006020:Glycopeptides / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013696:Temperature; D014859:Warfarin / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D006020:Glycopeptides; D010666:Phenylpropionates; C053759:eremomycin; D014859:Warfarin