title: T-cadherin in the mammalian cochlea.
abstract: OBJECTIVE
  Cadherins are a superfamily of transmembrane glycoproteins, which mediate calcium-dependent intercellular adhesions. T-cadherin is an atypical member of the cadherin family in regard to its structure; it acts as a signalling receptor rather than an adhesion molecule. In this study we examine the role of T-cadherin in the mammalian cochlea.
  
  
  METHODS
  This study investigated the expression of T-cadherin in the inner ear under physiologic and pathologic conditions.
  
  
  METHODS
  Expression of T-cadherin in the rat cochlea was analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR), real-time RT-PCR, Western blot, and immunohistochemistry.
  
  
  RESULTS
  We detected T-cadherin mRNA expression in three different components in the cochlea of postnatal mouse, namely the organ of Corti (OC), the spiral ganglion (SG), and the stria vascularis (SV). The SG and SV showed a higher T-cadherin mRNA level than the OC. T-cadherin protein was detected by Western blotting in the OC, SG, and SV. Immunofluorescence microscopy of adult mouse cochlea revealed the presence of T-cadherin in the apical parts of the inner and outer hair cells as well as in the SV and SG. OCs treated with gentamicin for 3, 6, or 12 hours did not show any change in T-cadherin gene expression compared to control explants maintained in culture medium alone.
  
  
  CONCLUSIONS
  T-cadherin is expressed within the cochlea. T-cadherin seems to have a wide variety of functions in the inner ear, ranging from mechanical functions to functions in response to hair cell damage and loss.
authors: Inner Ear Research Laboratory, Department of Biomedicine, University Hospital Basel, Basel, Switzerland.
authors: Alwin
authors: A
authors: Listyo
authors: Yves
authors: Y
authors: Brand
authors: Cristian
authors: C
authors: Setz
authors: Vesna
authors: V
authors: Radojevic
authors: Therese
authors: T
authors: Resink
authors: Soledad
authors: S
authors: Levano
authors: Daniel
authors: D
authors: Bodmer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism* / Q000502:physiology; D015834:Cochlear Diseases / Q000235:genetics; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D051379:Mice; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D015820:Cadherins; C099972:H-cadherin; D012333:RNA, Messenger