title: Structures of a putative ζ-class glutathione S-transferase from the pathogenic fungus Coccidioides immitis.
abstract: Coccidioides immitis is a pathogenic fungus populating the southwestern United States and is a causative agent of coccidioidomycosis, sometimes referred to as Valley Fever. Although the genome of this fungus has been sequenced, many operons are not properly annotated. Crystal structures are presented for a putative uncharacterized protein that shares sequence similarity with ζ-class glutathione S-transferases (GSTs) in both apo and glutathione-bound forms. The apo structure reveals a nonsymmetric homodimer with each protomer comprising two subdomains: a C-terminal helical domain and an N-terminal thioredoxin-like domain that is common to all GSTs. Half-site binding is observed in the glutathione-bound form. Considerable movement of some components of the active site relative to the glutathione-free form was observed, indicating an induced-fit mechanism for cofactor binding. The sequence homology, structure and half-site occupancy imply that the protein is a ζ-class glutathione S-transferase, a maleylacetoacetate isomerase (MAAI).
authors: Seattle Structural Genomics Center for Infectious Disease, USA. tedwards@embios.com
authors: Thomas E
authors: TE
authors: Edwards
authors: Cassie M
authors: CM
authors: Bryan
authors: David J
authors: DJ
authors: Leibly
authors: Shellie H
authors: SH
authors: Dieterich
authors: Jan
authors: J
authors: Abendroth
authors: Banumathi
authors: B
authors: Sankaran
authors: Dhileep
authors: D
authors: Sivam
authors: Bart L
authors: BL
authors: Staker
authors: Wesley C
authors: WC
authors: Van Voorhis
authors: Peter J
authors: PJ
authors: Myler
authors: Lance J
authors: LJ
authors: Stewart
mesh_terms: D001059:Apoproteins / Q000737:chemistry; D003045:Coccidioides / Q000201:enzymology*; D018360:Crystallography, X-Ray; D005982:Glutathione Transferase / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D040681:Structural Homology, Protein
chemical_list: D001059:Apoproteins; D005982:Glutathione Transferase