title: Ultraviolet B radiation to the eye induces pigmentation in the epidermis via the activation of the subunit gp91 phox of reduced nicotinamide adenine dinucleotide phosphate oxidase.
abstract: Irradiation by ultraviolet (UV)B is known to increase the number of dopamine (Dopa)-positive melanocytes in the skin. In this study, a 2.5-kJ/m(2)  dose of UVB radiation was delivered by a sunlamp to the ear or the eye of wild-type C57BL/6j mice and of gp91 phox(-/-)  C57BL/6j mice that had a knockout mutation of the gp91 phox subunit of reduced nicotinamide adenine dinucleotide phosphate oxidase (NADPH). The degree of change in the Dopa-positive melanocyte expression in was reduced in gp91 phox(-/-)  mice given UVB irradiation to the eye, but not in those given irradiation to the ear. The plasma level of α-melanocyte-stimulating hormone (α-MSH) in the blood increased in the C57BL/6j mice after irradiation to either the eye or the ear, but it did not increase in the gp91 phox(-/-)  mice given UVB irradiation to the eye. Both gp91 phox and α-MSH in the central nervous system seem to contribute to pigmentation after UVB irradiation of the eye in mice.
authors: Department of Biochemistry and Molecular Pathology, Osaka City University Medical School, Osaka, Japan. m9378012@msic.med.osaka-cu.ac.jp
authors: K
authors: K
authors: Hiramoto
authors: E F
authors: EF
authors: Sato
mesh_terms: D000818:Animals; D005123:Eye / Q000528:radiation effects*; D008562:Membrane Glycoproteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D019255:NADPH Oxidases / Q000502:physiology*; D012880:Skin Pigmentation / Q000528:radiation effects*; D014466:Ultraviolet Rays; D000521:alpha-MSH / Q000097:blood
chemical_list: D008562:Membrane Glycoproteins; D000521:alpha-MSH; D019255:NADPH Oxidases