title: Emulsion electrospinning of a collagen-like protein/PLGA fibrous scaffold: empirical modeling and preliminary release assessment of encapsulated protein.
abstract: The effectiveness of a multifunctional scaffold produced by the electrospinning of emulsions composed of organic PLGA and aqueous collagen-like protein (denoted as Fol-8Col) solutions is demonstrated. The resultant Fol-8Col/PLGA fibrous scaffolds with homogeneous morphology have mean fiber diameters from 600 to 2,000 nm. A uniform distribution of encapsulated Fol-8Col in the fibers is observed by fluorescence microscopy. TEM is used to clarify the representative core/sheath structure of emulsion electrospun Fol-8Col/PLGA fibers. Preliminary release assessment of encapsulated Fol-8Col shows results of sustained release for more than one month from the Fol-8Col/PLGA fibrous mats. The cytocompatibility of fibroblast cell line L929 with the fibrous composite seems promosing.
authors: Faculty of Textile Science and Technology, Shinshu University, Japan.
authors: Kai
authors: K
authors: Wei
authors: Yuan
authors: Y
authors: Li
authors: Xiaowen
authors: X
authors: Lei
authors: Heejae
authors: H
authors: Yang
authors: Akira
authors: A
authors: Teramoto
authors: Juming
authors: J
authors: Yao
authors: Koji
authors: K
authors: Abe
authors: Frank K
authors: FK
authors: Ko
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000494:pharmacology; D004655:Emulsions / Q000737:chemistry; D004926:Escherichia coli; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D007700:Kinetics; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000494:pharmacology; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011100:Polyglycolic Acid / Q000138:chemical synthesis* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004655:Emulsions; D011994:Recombinant Proteins; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D003094:Collagen