title: 1-oxoeudesm-11(13)-ene-12,8α-lactone-induced apoptosis via ROS generation and mitochondria activation in MCF-7 cells.
abstract: A novel eudesmane-type sesquiterpene compound, 1-oxoeudesm-11(13)-eno-12,8a-lactone (OEL), was isolated from Aster himalaicus. Its effect on apoptosis in human breast adenocarcinoma (MCF-7) cells was investigated. MTT assay showed that OEL substantially reduced the viability of KB, MCF-7, U87, A172, and MG-63 cells. MCF-7 cells were used to further evaluate the antitumor effects and anticancer mechanisms of OEL. OEL-induced apoptosis was characterized by chromatin condensation, formation of apoptotic bodies, and phosphatidylserine on extracellular surface; these effects were confirmed by DAPI nuclear staining and flow cytometry. Increased expression of Bax and deceased expression of Bcl-2 were also observed in OEL-treated MCF-7 cells. Moreover, OEL induced the loss of mitochondria membrane potential, release of cytochrome C, activation of caspase-9, and generation of reactive oxygen species. These findings indicate that reactive oxygen species generation and mitochondria activation were involved in apoptosis induced by OEL in MCF-7 cells. The results from our study demonstrated that OEL may be a promising pro-apoptotic compound that could be used to develop novel anticancer drugs.
authors: School of Ocean, Shandong University, Weihai 264209, China.
authors: Min
authors: M
authors: Cui
authors: Yahong
authors: Y
authors: Zhang
authors: Shanshan
authors: S
authors: Liu
authors: Weidong
authors: W
authors: Xie
authors: Mei
authors: M
authors: Ji
authors: Hongxiang
authors: H
authors: Lou
authors: Xia
authors: X
authors: Li
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D001943:Breast Neoplasms / Q000188:drug therapy; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D002470:Cell Survival; D004354:Drug Screening Assays, Antitumor; D005260:Female; D022781:Hepatocytes; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007211:Indoles / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial; D008928:Mitochondria / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D045787:Sesquiterpenes, Eudesmane / Q000494:pharmacology*; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: C548435:1-oxoeudesm-11(13)-eno-12,8a-lactone; D000970:Antineoplastic Agents; D007211:Indoles; D017382:Reactive Oxygen Species; D045787:Sesquiterpenes, Eudesmane; D013778:Tetrazolium Salts; D013844:Thiazoles; C007293:DAPI; C022616:thiazolyl blue