title: [Synthesis and identification of the hapten and complete antigens for Zearalenone].
abstract: OBJECTIVE
  To synthesize and identify the hapten and complete antigens for Zearalenone for the purpose of preparation of anti-Zearalenone antibodies.
  
  
  METHODS
  Zearalenone and carboxymethoxylamine hemihydrochloride were incubated to synthesize the hapten ZEN-oxime. The ZEN-oxime was identified by thin layer chromatography (TLC), high performance liquid chromatographic (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MSn). The ZEN-oxime was further conjugated to bovine serum albumin (BSA) or ovalbumin (OVA) to form the complete antigens by NHS ester method. The complete antigens were identified by ultraviolet spectrum, 2, 4, 6-trinitrobenzene sulfonicacid method (TNBS) and immunological analysis.
  
  
  RESULTS
  The results indicated that the hapten and complete antigens were prepared successfully.
  
  
  CONCLUSIONS
  The hapten and complete antigens for Zearalenone have been successfully synthesized and identified by several methods, which enables further preparation of anti-Zearalenone antibodies.
authors: College of Life Science, Nanjing Normal University, Nanjing 210046, China.
authors: Huan
authors: H
authors: Cao
authors: Fang
authors: F
authors: Ji
authors: Xiu-Yu
authors: XY
authors: Wang
authors: Jian-Rong
authors: JR
authors: Shi
mesh_terms: D000941:Antigens / Q000737:chemistry* / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006241:Haptens / Q000737:chemistry* / Q000276:immunology*; D013058:Mass Spectrometry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D015025:Zearalenone / Q000737:chemistry* / Q000276:immunology*
chemical_list: D000941:Antigens; D006241:Haptens; D012710:Serum Albumin, Bovine; D015025:Zearalenone