title: Novel conductometric biosensor based on three-enzyme system for selective determination of heavy metal ions.
abstract: A differential pair of planar thin-film interdigitated electrodes, deposited on a ceramic pad, was used as a conductometric transducer. The three-enzyme system (invertase, mutarotase, glucose oxidase), immobilized on the transducer surface, was used as a bioselective element. The ratio between enzymes in the membrane was found experimentally considering the highest biosensor sensitivity to substrate (sucrose) and heavy metal ions. Optimal concentration of sucrose for inhibitory analysis was 1.25 mM and incubation time in the investigated solution amounted to 10-20 min. The developed biosensor demonstrated the best sensitivity toward ions Hg(2+) and Ag(+). A principal possibility of the biosensor reactivation either by EDTA solution after inhibition with silver ions or by cysteine solution after inhibition with mercury ions was shown.
authors: Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, Zabolotnogo Street 150, 03143 Kyiv, Ukraine. alex_sold@yahoo.com
authors: O O
authors: OO
authors: Soldatkin
authors: I S
authors: IS
authors: Kucherenko
authors: V M
authors: VM
authors: Pyeshkova
authors: A L
authors: AL
authors: Kukla
authors: N
authors: N
authors: Jaffrezic-Renault
authors: A V
authors: AV
authors: El'skaya
authors: S V
authors: SV
authors: Dzyadevych
authors: A P
authors: AP
authors: Soldatkin
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002238:Carbohydrate Epimerases / Q000737:chemistry / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry; D003217:Conductometry / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D017743:Equipment Reuse; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D008628:Mercury / Q000032:analysis*; D012680:Sensitivity and Specificity; D012834:Silver / Q000032:analysis*; D013395:Sucrose / Q000378:metabolism; D014159:Transducers; D043324:beta-Fructofuranosidase / Q000737:chemistry / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D012834:Silver; D013395:Sucrose; D005949:Glucose Oxidase; D043324:beta-Fructofuranosidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase; D008628:Mercury