title: Preparation of a polypyrrole-polyvinylsulphonate composite film biosensor for determination of phenol based on entrapment of polyphenol oxidase.
abstract: Abstract: In this paper, a novel amperometric phenol biosensor with immobilization of polyphenol oxidase (tyrosinase) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique on the surface of a platinum electrode. The amperometric determination is based on the electrochemical reduction of quinon generated in the enzymatic reaction of phenol. The effects of pH and temperature were investigated and optimum parameters were found to be 8.0 and 30 °C, respectively. The linear working range of the electrode was 1.0 × 10(-7) - 5.0 × 10(-6) M. The storage stability and operation stability of the enzyme electrode were also studied.
authors: Department of Chemistry, Gazi University, Ankara, Turkey. halit@gazi.edu.tr
authors: Halit
authors: H
authors: Arslan
authors: Fatma
authors: F
authors: Arslan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004156:Catechol Oxidase / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000032:analysis*; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D011199:Potentiometry; D011758:Pyrroles / Q000737:chemistry; D011809:Quinones / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D010636:Phenols; D011108:Polymers; D011145:Polyvinyls; D011758:Pyrroles; D011809:Quinones; D013451:Sulfonic Acids; C008959:lyapolate; C067635:polypyrrole; D010984:Platinum; D004156:Catechol Oxidase