title: DNA reorientation on Au nanoparticles: label-free detection of hybridization by surface enhanced Raman spectroscopy.
abstract: DNA sequences attached to Au nanoparticles via thiol linkers stand up from the surface, giving preferential enhancement of the adenine ring breathing SERS band. Non-specific binding via the nucleobases reorients the DNA, reducing this effect. This change in intensity on reorientation was utilised for label-free detection of hybridization of a molecular beacon.
authors: Innovative Molecular Materials Group, School of Chemistry and Chemical Engineering, Queen's University, Belfast, BT9 5AG, UK.
authors: Evanthia
authors: E
authors: Papadopoulou
authors: Steven E J
authors: SE
authors: Bell
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry / Q000235:genetics; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D015345:Oligonucleotide Probes; D006046:Gold; D004247:DNA