title: Structure and phase behavior of archaeal lipid monolayers.
abstract: We report X-ray reflectivity (XRR) and grazing incidence X-ray diffraction (GIXD) measurements of archaeal bipolar tetraether lipid monolayers at the air-water interface. Specifically, Langmuir films made of the polar lipid fraction E (PLFE) isolated from the thermoacidophilic archaeon  Sulfolobus acidocaldarius  grown at three different temperatures, i.e., 68, 76, and 81 °C, were examined. The dependence of the structure and packing properties of PLFE monolayers on surface pressure were analyzed in a temperature range between 10 and 50 °C at different pH values. Additionally, the interaction of PLFE monolayers (using lipids derived from cells grown at 76 °C) with the ion channel peptide gramicidin was investigated as a function of surface pressure. A total monolayer thickness of approximately 30 Å was found for all monolayers, hinting at a U-shaped conformation of the molecules with both head groups in contact with the interface. The monolayer thickness increased with rising film pressure and decreased with increasing temperature. At 10 and 20 °C, large, highly crystalline domains were observed by GIXD, whereas at higher temperatures no distinct crystallinity could be observed. For lipids derived from cells grown at higher temperatures, a slightly more rigid structure in the lipid dibiphytanyl chains was observed. A change in the pH of the subphase had an influence only on the structure of the lipid head groups. The addition of gramicidin to an PLFE monolayer led to a more disordered state as observed by XRR. In GIXD measurements, no major changes in lateral organization could be observed, except for a decrease of the size of crystalline domains, indicating that gramicidin resides mainly in the disordered areas of the monolayer and causes local membrane perturbation, only.
authors.affiliation: Physical Chemistry I, Faculty of Chemistry, TU Dortmund University, Dortmund, Otto-Hahn-Strasse 6, D-44221 Dortmund, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Jeworrek
authors.forename: Florian
authors.initials: F
authors.lastname: Evers
authors.forename: Mirko
authors.initials: M
authors.lastname: Erlkamp
authors.forename: Sebastian
authors.initials: S
authors.lastname: Grobelny
authors.forename: Metin
authors.initials: M
authors.lastname: Tolan
authors.forename: Parkson Lee-Gau
authors.initials: PL
authors.lastname: Chong
authors.forename: Roland
authors.initials: R
authors.lastname: Winter
mesh_terms: D000388:Air; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D049109:Cell Proliferation; D006096:Gramicidin / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D016944:Sulfolobus / Q000737:chemistry* / Q000166:cytology; D013499:Surface Properties; D013696:Temperature; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D008055:Lipids; D014867:Water; D006096:Gramicidin