title: [Effects of maleic anhydride-modified poly(D,L-lactic acid) on the adhesion, proliferation and differentiation of osteoblasts].
abstract: The main objective of this study was to observe the adhesion, proliferation and differentiation of mouse osteblast-like MC3T3-E1 cells cultured on maleic anhydride-modified poly(D,L-lactic acid) (MPLA) and poly(D,L-lactic acid) (PDLLA) polymers, and to evaluate the cytocompatibility of MPLA polymer. The effects of MPLA and PDLLA polymers on the morphology, adhesion, proliferation, the content of total cellular protein, alkaline phosphatase (ALP) activity and the content of Ca of MC3T3-E1 cells were explored. These results indicated that MC3T3-E1 cells on MPLA polymer adhered and spread more fully. On MPLA polymer, the proliferation, total protein content, ALP activity, Ca content of the cells were significantly higher than those of the cells on PDLLA polymer (P < 0.01). It was concluded that MPLA polymer could promote the adhesion, spreading, proliferation and the synthesis of protein of osteoblasts, and also induced the differentiation and mineralization of osteoblasts, suggesting that MPLA polymer might have the better cytocompatibility than PDLLA.
authors.affiliation: Research Center of Bioinspired Materials Science and Engineering, Bioengineering College, Chongqing University, Chongqing 400044, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiang
authors.forename: YuanLiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Luo
authors.forename: Bingbing
authors.initials: B
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Xin
authors.forename: Danfang
authors.initials: D
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004622:Embryo, Mammalian; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D008299:Maleic Anhydrides / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D008299:Maleic Anhydrides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)