title: Tumor suppressive microRNA-375 regulates lactate dehydrogenase B in             maxillary sinus squamous cell carcinoma.
abstract: The expression of microRNA-375 (miR-375) is significantly reduced in cancer             tissues of maxillary sinus squamous cell carcinoma (MSSCC). The aim of this study             was to investigate the functional significance of miR-375 and a possible regulatory             role in the MSSCC networks. Restoration of miR-375 significantly inhibited cancer             cell proliferation and invasion in IMC-3 cells, suggesting that miR-375 functions             as a tumor suppressor in MSSCC. Genome-wide gene expression data and luciferase             reporter assays indicated that lactate dehydro-genase B (LDHB) was directly regulated             by miR-375. Cancer cell proliferation and invasion were significantly inhibited             by transfection of si-LDHB into IMC-3 cells, suggesting that LDHB may play a role             in MSSCC oncogenic function. In clinical MSSCC specimens, LDHB mRNA levels were             up-regulated in cancer tissues, which were inversely correlated with the expression             of miR-375. In addition, Kaplan-Meier curves and log-rank tests revealed that             the high mRNA expression levels of LDHB had a significant adverse effect on survival             rate. The identification of a cancer network regulated by the miR-375 tumor suppressor             could provide new insights into the molecular mechanisms of MSSCC oncogenesis.
authors.affiliation: Department of Functional Genomics, Chiba University Graduate                     School of Medicine, 1-8-1 Inohana Chuo-ku, Chiba 260-8670, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kinoshita
authors.forename: Nijiro
authors.initials: N
authors.lastname: Nohata
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshino
authors.forename: Toyoyuki
authors.initials: T
authors.lastname: Hanazawa
authors.forename: Naoko
authors.initials: N
authors.lastname: Kikkawa
authors.forename: Lisa
authors.initials: L
authors.lastname: Fujimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Chiyomaru
authors.forename: Kazumori
authors.initials: K
authors.lastname: Kawakami
authors.forename: Hideki
authors.initials: H
authors.lastname: Enokida
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Okamoto
authors.forename: Naohiko
authors.initials: N
authors.lastname: Seki
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics* / Q000628:therapy; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008444:Maxillary Sinus Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000628:therapy; D035683:MicroRNAs / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D014162:Transfection
chemical_list: D007527:Isoenzymes; C540933:MIRN375 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1