title: "Free" copper: a new endogenous chemical mediator of inflammation in birds.
abstract: For acceptance of any chemical agent as an endogenous chemical mediator of inflammation, the agent in question must fulfill some biological requirements which are (a) it should be ubiquitously present in tissues in inactive form, (b) it should be activated during process of inflammation whose increase should be identifiable, (c) it should induce or amplify some events of inflammation, (d) there must be some natural inhibitor of such active form in tissues, (e) it should be able to induce inflammatory reaction after exogenous injection, (f) such reaction should be inhibited by exogenous use of their antagonists, and (g) it should be amplified by use of agonists. Copper in its protein free or protein bound form are reported to act as pathogenic factor in inflammatory processes due to oxidative stress. But their role as endogenous chemical mediator of inflammation does not appear to be investigated thoroughly in light of abovementioned biological criterion of mediator. Present study aims at thorough exploration on role of free copper as endogenous chemical mediator of inflammation in light of above facts. It was done by estimation of total copper, protein-bound copper, and free copper along with estimation of free radical generation, increase in vascular permeability, and cellular infiltration during acute inflammatory reaction induced by carrageenan and concanavalin using chicken skin as test model. It was further evaluated by use of exogenous free copper in experimental model and their subsequent inhibition and amplification by chemical chelators of copper. Present study confirms that free copper fulfilled all the biological requirements for accepting it as an endogenous chemical mediator of inflammation.
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Science & AH, Birsa Agricultural University, Ranchi, 834006 Jharkhand, India. singhkkpatho@gmail.com
authors.forename: Kaushal Kumar
authors.initials: KK
authors.lastname: Singh
authors.forename: Mukesh
authors.initials: M
authors.lastname: Kumar
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.forename: Madhurendu Kumar
authors.initials: MK
authors.lastname: Gupta
authors.forename: Dilip Kumar
authors.initials: DK
authors.lastname: Jha
authors.forename: Suruchi
authors.initials: S
authors.lastname: Kumari
authors.forename: Birendra Kumar
authors.initials: BK
authors.lastname: Roy
authors.forename: Sanjit
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001717:Birds; D001808:Blood Vessels / Q000378:metabolism; D003300:Copper / Q000032:analysis* / Q000493:pharmacokinetics; D005609:Free Radicals; D018836:Inflammation Mediators / Q000032:analysis* / Q000493:pharmacokinetics
chemical_list: D005609:Free Radicals; D018836:Inflammation Mediators; D003300:Copper