title: Seeking sense of antisense switch transcripts.
abstract: In B lymphocytes, class switch recombination (CSR) machinery targets highly repetitive sequences, called switch (S) sequences, in the constant domain of the immunoglobulin heavy chain (IgH) locus. Cotranscriptional generation of R loops at S sequences provides the substrate for the mutagenic enzyme AID (Activation-Induced cytidine Deaminase), which initiates the DNA breaks at the transcribed sequences. Both sense and antisense transcripts across the S regions have been reported. Our recent work shows that, unlike its sense counterpart, antisense transcription of S sequences is dispensable for CSR in vivo.
authors.affiliation: CNRS UMR 5089-IPBS (Institut de Pharmacologie et de Biologie Structurale) and Université Paul Sabatier III; Equipe "Instabilité Génétique et Régulation Transcriptionnelle"; Université de Toulouse; Toulouse, France.
authors.forename: Dania
authors.initials: D
authors.lastname: Haddad
authors.forename: Nadine
authors.initials: N
authors.lastname: Puget
authors.forename: Nathalie
authors.initials: N
authors.lastname: Laviolette-Malirat
authors.forename: Caroline
authors.initials: C
authors.lastname: Conte
authors.forename: Ahmed Amine
authors.initials: AA
authors.lastname: Khamlichi