title: A zebrafish model of dyskeratosis congenita reveals hematopoietic stem cell formation failure resulting from ribosomal protein-mediated p53 stabilization.
abstract: Dyskeratosis congenita (DC) is a bone marrow failure disorder characterized by shortened telomeres, defective stem cell maintenance, and highly heterogeneous phenotypes affecting predominantly tissues that require high rates of turnover. Here we present a mutant zebrafish line with decreased expression of nop10, one of the known H/ACA RNP complex genes with mutations linked to DC. We demonstrate that this nop10 loss results in 18S rRNA processing defects and collapse of the small ribosomal subunit, coupled to stabilization of the p53 tumor suppressor protein through small ribosomal proteins binding to Mdm2. These mutants also display a hematopoietic stem cell deficiency that is reversible on loss of p53 function. However, we detect no changes in telomere length in nop10 mutants. Our data support a model of DC whereupon in early development mutations involved in the H/ACA complex contribute to bone marrow failure through p53 deregulation and loss of initial stem cell numbers while their role in telomere maintenance does not contribute to DC until later in life.
authors.affiliation: Hubrecht Institute, Koninklijke Nederlandse Akademie van Wetenschappen and Universitair Medisch Centrum Utrecht, Uppsalalaan 8, Utrecht, The Netherlands.
authors.forename: Tamara C
authors.initials: TC
authors.lastname: Pereboom
authors.forename: Linda J
authors.initials: LJ
authors.lastname: van Weele
authors.forename: Albert
authors.initials: A
authors.lastname: Bondt
authors.forename: Alyson W
authors.initials: AW
authors.lastname: MacInnes
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D004195:Disease Models, Animal; D019871:Dyskeratosis Congenita / Q000097:blood* / Q000235:genetics / Q000473:pathology; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000473:pathology* / Q000502:physiology*; D010641:Phenotype; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000502:physiology; D017411:Ribonucleoproteins, Small Nuclear / Q000235:genetics* / Q000378:metabolism; D020718:Ribonucleoproteins, Small Nucleolar / Q000235:genetics* / Q000378:metabolism; D054682:Ribosome Subunits, Small, Eukaryotic / Q000502:physiology; D012270:Ribosomes / Q000502:physiology; D016615:Telomere / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C569299:NOP10 protein, zebrafish; D012337:RNA, Ribosomal, 18S; D017411:Ribonucleoproteins, Small Nuclear; D020718:Ribonucleoproteins, Small Nucleolar; D016159:Tumor Suppressor Protein p53; D029961:Zebrafish Proteins; D051736:Proto-Oncogene Proteins c-mdm2; C497209:mdm2 protein, zebrafish