title: Serial changes in cytokine expression in irritable bowel syndrome patients following treatment with calcium polycarbophil.
abstract: OBJECTIVE
  Calcium polycarbophil improves abdominal symptoms in patients with irritable bowel syndrome (IBS). We examined cytokine expression in IBS patients before and after administration of calcium polycarbophil.
  
  
  METHODS
  A total of 24 IBS patients (13 diarrhea type, 11 constipation type; median age, 55 years) were enrolled. Serum levels of high sensitive C-reactive protein (CRP) and 17 cytokines (interleukin [IL]-1ß, -2, -4, 5, -6, -7, -8, -10, -12, -13 and -17; tumor necrosis factor-a [TNF-a]; interferon [IFN]-?; granulocyte colony-stimulating factor [G-CSF]; granulocyte macrophage colony-stimulating factor [GM-CSF]; macrophage inflammatory protein [MIP]-1ß; and macrophage chemo-attractant protein [MCP-1]) were simultaneously determined using a Bio-Plex suspension array system before and 12 weeks after administration of calcium polycarbophil 1,500-3,000mg/day.
  
  
  RESULTS
  Serum MCP-1 levels in diarrhea type IBS patients were significantly higher than those in constipation type patients (p<0.05). In IBS patients, no significant changes in serum cytokine levels were observed following calcium polycarbophil administration. In constipation type patients, serum high sensitive CRP levels were significantly lower after treatment than before treatment.
  
  
  CONCLUSIONS
  Decreases in serum high sensitive CRP levels following calcium polycarbophil treatment may be involved in the relief of abdominal symptoms in IBS patients; diarrhea type IBS is characterized by increased MCP-1 expression.
authors.affiliation: Department of Gastroenterology and Hepatology, Iwate Medical University, Iwate, Japan. toschiba@iwate-med.ac.jp
authors.forename: Toshimi
authors.initials: T
authors.lastname: Chiba
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Sato
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Toya
authors.forename: Kei
authors.initials: K
authors.lastname: Endo
authors.forename: Yukito
authors.initials: Y
authors.lastname: Abiko
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kasugai
authors.forename: Shinji
authors.initials: S
authors.lastname: Saito
authors.forename: Shuhei
authors.initials: S
authors.lastname: Oana
authors.forename: Norihiko
authors.initials: N
authors.lastname: Kudara
authors.forename: Masaki
authors.initials: M
authors.lastname: Endo
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000180:Acrylic Resins / Q000627:therapeutic use*; D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D000930:Antidiarrheals / Q000627:therapeutic use*; D002097:C-Reactive Protein / Q000378:metabolism; D002400:Cathartics / Q000627:therapeutic use*; D018932:Chemokine CCL2 / Q000097:blood; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000097:blood* / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged
chemical_list: D000180:Acrylic Resins; D000930:Antidiarrheals; D002400:Cathartics; D018932:Chemokine CCL2; D016207:Cytokines; C030312:calcium polycarbophil; D002097:C-Reactive Protein