title: [Comparison of mtDNA extracting methods for common sarcosaphagous insects].
abstract: OBJECTIVE
  To compare effects of three different methods for mtDNA extraction from common sarcosaphagous insects including cetyl trimethyl ammonium bromide (CTAB) method, sodium dodecyl sulfate-potassium acetate (SDS-KAc) method and sodium dodecyl sulfate-proteinase K (SDS-PK) method.
  
  
  METHODS
  Seventy-two insects from four species [Chrysomya megacephala (Fabricius, 1784), Eusilpha bicolor (Fairmaire, 1896), Paraeutrichopus pecoudi (Mateu, 1954), Vespa velutina (Lepeletier, 1836)] were collected from the corpses of the rabbits in Changsha district. The total DNA of above samples was extracted by CTAB, SDS-Kac and SDS-PK methods. The purity and concentration of DNA were examined by protein-nucleic acid spectrophotometry, and mtDNA were amplified by specific primers and PCR products were detected by agarose gel electrophoresis. Then PCR products were sequenced and subsequently up-loaded to GenBank.
  
  
  RESULTS
  mtDNA was successfully extracted with three methods from most of the samples. The SDS-PK method was better in DNA purity compared to other methods and the CTAB method was superior in extracting DNA from old samples, while SDS-KAc method showed no significant difference for extraction effects of different samples.
  
  
  CONCLUSIONS
  The most appropriate method should be chosen depending on different situations. SDS-PK method is expected to obtain high-quality DNA, while CTAB method is preferred in extracting obsolete samples. SDS-KAc method is low cost and can be used in various kinds of preliminary experiments.
authors.affiliation: Hunan Police College, Changsha 410138, China. chenyaoqing1994@21cn.com
authors.forename: Yao-Qing
authors.initials: YQ
authors.lastname: Chen
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Guo
authors.forename: Mao-Zhi
authors.initials: MZ
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Xiong
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Li
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Cai
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D004175:Diptera / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004772:Entomology; D005554:Forensic Medicine / Q000379:methods*; D005784:Gene Amplification; D007313:Insecta / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D011817:Rabbits; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D000644:Quaternary Ammonium Compounds; C521669:cetyl ammonium bromide; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate