title: Characteristic CD103 and CD123 expression pattern defines hairy cell leukemia: usefulness of CD123 and CD103 in the diagnosis of mature B-cell lymphoproliferative disorders.
abstract: By using flow cytometry, we studied CD103 and CD123 expression by the malignant cells in 300 B-cell lymphoproliferative disorder (BC-LPD) cases, including 114 hairy cell leukemia (HCL), 20 HCL variant (HCLv), 9 splenic marginal zone lymphoma (SMZL; in 5, only CD103 was evaluated), 133 chronic lymphocytic leukemia (CLL), 3 follicular lymphoma (FL), and 21 mantle cell lymphoma (MCL). All HCLs expressed uniform CD103 and bright CD123. Among the 20 HCLv cases, 20 (100%) were CD103+ and 8 (40%) were CD123+ (partial or dim). CD103 was negative in all MCL, FL, CLL, and SMZL cases. CD123 was positive in 1 (25%) of 4 SMZL, 3.8% of CLL (5/133), 7 (33%) of 21 MCL, and 1 (33%) of 3 FL cases. CD103 is specific for HCL and HCLv. CD123 expression is more widespread in BC-LPDs but is useful in conjunction with CD25 to differentiate HCLv from HCL. These findings support the usefulness of CD123 and CD103 to aid in the differential diagnosis of BC-LPDs.
authors.affiliation: National Cancer Institute, 10 Center Drive, Bethesda, MD 20892, USA.
authors.forename: Girish
authors.initials: G
authors.lastname: Venkataraman
authors.forename: Christine
authors.initials: C
authors.lastname: Aguhar
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kreitman
authors.forename: Constance M
authors.initials: CM
authors.lastname: Yuan
authors.forename: Maryalice
authors.initials: M
authors.lastname: Stetler-Stevenson
mesh_terms: D015703:Antigens, CD / Q000032:analysis* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis*; D002469:Cell Separation; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D039001:Integrin alpha Chains / Q000032:analysis* / Q000378:metabolism; D053650:Interleukin-3 Receptor alpha Subunit / Q000032:analysis* / Q000378:metabolism; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000378:metabolism; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C115022:IL3RA protein, human; D039001:Integrin alpha Chains; D053650:Interleukin-3 Receptor alpha Subunit; C091367:alpha E integrins