title: Characterization of a novel β-L-Arabinofuranosidase in Bifidobacterium longum: functional elucidation of A DUF1680 family member.
abstract: Pfam DUF1680 (PF07944) is an uncharacterized protein family conserved in many species of bacteria, actinomycetes, fungi, and plants. In a previous article, we cloned and characterized the hypBA2 gene as a β-l-arabinobiosidase in Bifidobacterium longum JCM 1217. In this study, we cloned a DUF1680 family member, the hypBA1 gene, which constitutes a gene cluster with hypBA2. HypBA1 is a novel β-l-arabinofuranosidase that liberates l-arabinose from the l-arabinofuranose (Araf)-β1,2-Araf disaccharide. HypBA1 also transglycosylates 1-alkanols with retention of the anomeric configuration. Mutagenesis and azide rescue experiments indicated that Glu-366 is a critical residue for catalytic activity. This report provides the first characterization of a DUF1680 family member, which defines a new family of glycoside hydrolases, the GH family 127.
authors.affiliation: Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan. Electronic address: kfujita@ms.kagoshima-u.ac.jp.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Fujita
authors.affiliation: Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Takashi
authors.affiliation: Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Obuchi
authors.affiliation: Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Kanefumi
authors.initials: K
authors.lastname: Kitahara
authors.affiliation: Faculty of Agriculture, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Suganuma
mesh_terms: D001644:Bifidobacterium / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006031:Glycosylation; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009844:Oligosaccharides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D013997:Time Factors
chemical_list: D009844:Oligosaccharides; D011108:Polymers; D006026:Glycoside Hydrolases