title: A multivalent probe for AI-2 quorum-sensing receptors.
abstract: Multivalency is a common principle in the recognition of cellular receptors, and multivalent agonists and antagonists have played a major role in understanding mammalian cell receptor biology. The study of bacterial cell receptors using similar approaches, however, has lagged behind. Herein we describe our efforts toward the development of a dendrimer-based multivalent probe for studying AI-2 quorum-sensing receptors. From these studies, we have discovered a chemical probe specific for Lsr-type AI-2 quorum-sensing receptors with the potential for enabling the identification of new bacterial species that utilize AI-2 as a quorum-sensing signaling molecule.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
authors.forename: Amanda L
authors.initials: AL
authors.lastname: Garner
authors.forename: Junguk
authors.initials: J
authors.lastname: Park
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Zakhari
authors.forename: Colin A
authors.initials: CA
authors.lastname: Lowery
authors.forename: Anjali Kumari
authors.initials: AK
authors.lastname: Struss
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sawada
authors.forename: Gunnar F
authors.initials: GF
authors.lastname: Kaufmann
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
mesh_terms: D001409:Bacillus cereus / Q000166:cytology; D001419:Bacteria / Q000166:cytology*; D050091:Dendrimers / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D008958:Models, Molecular; D010420:Pentanes / Q000737:chemistry*; D053038:Quorum Sensing; D012235:Rhodamines / Q000737:chemistry; D012486:Salmonella typhimurium / Q000166:cytology; D014733:Vibrio / Q000166:cytology
chemical_list: C493138:4,5-dihydroxy-2,3-pentanedione; D050091:Dendrimers; D005456:Fluorescent Dyes; D010420:Pentanes; D012235:Rhodamines