title: Immunoreactivity of c-kit receptor protein during the prehibernation period in the oviduct of the Chinese brown frog, Rana chensinensis.
abstract: The objective of this study was to investigate immunoreactivity of the c-kit receptor in the oviduct of Rana chensinensis during the prehibernation period. Histological examination of oviducts was performed during the prehibernation period. The sections of oviduct were immunostained by the avidin-biotin-peroxidase complex method using rabbit polyclonal antisera raised against the rat c-kit receptor and PCNA. Total proteins were extracted from oviducal tissues and used for Western blotting analysis. Immunohistochemistry revealed the presence of the c-kit receptor and PCNA in the oviduct tissues during the prehibernation period. Also, positive signals for the c-kit receptor and PCNA by Western blotting were observed in oviduct tissues during the prehibernation period. These results suggested that the c-kit receptor might play a regulatory role in oviducal hypertrophy in the brown frog, Rana chensinensis.
authors.affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shen
authors.forename: Yuning
authors.initials: Y
authors.lastname: Liu
authors.forename: Jiajia
authors.initials: J
authors.lastname: Ma
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Ma
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.forename: Haolin
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Meiyu
authors.initials: M
authors.lastname: Xu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.forename: Gen
authors.initials: G
authors.lastname: Watanabe
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Taya
mesh_terms: D000818:Animals; D005260:Female; D006605:Hibernation / Q000276:immunology*; D007150:Immunohistochemistry / Q000662:veterinary; D010057:Oviducts / Q000276:immunology*; D019009:Proto-Oncogene Proteins c-kit / Q000276:immunology*; D011898:Ranidae / Q000276:immunology*
chemical_list: D019009:Proto-Oncogene Proteins c-kit