title: Triacylglycerol profiling of microalgae strains for biofuel feedstock by liquid chromatography-high-resolution mass spectrometry.
abstract: Biofuels from photosynthetic microalgae are quickly gaining interest as a viable carbon-neutral energy source. Typically, characterization of algal feedstock involves breaking down triacylglycerols (TAG) and other intact lipids, followed by derivatization of the fatty acids to fatty acid methyl esters prior to analysis by gas chromatography (GC). However, knowledge of the intact lipid profile could offer significant advantages for discovery stage biofuel research such as the selection of an algal strain or the optimization of growth and extraction conditions. Herein, lipid extracts from microalgae were directly analyzed by ultra-high pressure liquid chromatography-mass spectrometry (UHPLC-MS) using a benchtop Orbitrap mass spectrometer. Phospholipids, glycolipids, and TAGs were analyzed in the same chromatographic run, using a combination of accurate mass and diagnostic fragment ions for identification. Using this approach, greater than 100 unique TAGs were identified over the six algal strains studied and TAG profiles were obtained to assess their potential for biofuel applications. Under the growth conditions employed, Botryococcus braunii and Scenedesmus obliquus yielded the most comprehensive TAG profile with a high abundance of TAGs containing oleic acid.
authors.affiliation: National Research Council of Canada, Institute for Marine Biosciences, 1411 Oxford St., Halifax, NS B3H 3Z1, Canada.
authors.forename: Karen M
authors.initials: KM
authors.lastname: MacDougall
authors.forename: Jesse
authors.initials: J
authors.lastname: McNichol
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: McGinn
authors.forename: Stephen J B
authors.initials: SJ
authors.lastname: O'Leary
authors.forename: Jeremy E
authors.initials: JE
authors.lastname: Melanson
mesh_terms: D056804:Biofuels / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D058086:Microalgae / Q000737:chemistry*; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D056804:Biofuels; D014280:Triglycerides