title: Sodium arsenite dependent protein expression analysis on human embryonic carcinoma (NCCIT) cell line.
abstract: Due to their pluripotent nature, embryonic carcinoma (EC) cell lines are useful for the study of basic and applied aspects of medical therapeutics, disease management and environmental mutagenesis. We evaluated the teratogenic like effects of inorganic arsenic during the early stages of cellular development in NCCIT cells, a type of human EC cells. Using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and MetaCore pathway analysis software (GeneGo), the proteomic expression profiles of NCCIT cells after either short- or long-term sodium arsenite (NaAsO(2)) treatment and of NCCIT cell-derived embryoid bodies (EBs) after short-term NaAsO(2) treatment were studied to determine the toxic effects on the process of normal growth and differentiation. The protein expression profiles of the NaAsO(2)-treated NCCIT cells and EBs were compared with that of untreated NCCIT cells. Short-term NaAsO(2) treatment resulted in the down-regulation of most proteins, with heat shock 90-kDa protein (HSP90) and valosin-containing protein (VCP) being significantly downregulated. Long-term NaAsO(2) treatment caused marked up-regulation of B23/nucleophosmin, glucose regulated protein 78-kDa (GRP78), unc-51-like kinase 3 (ULK3), toll-like receptor 6 precursor (TLR6) and mitogen-activated protein kinase 7 isoform A (MAP3K7). NaAsO(2) treatment of the NCCIT cell-derived EBs resulted in the down-regulation of several tumor-suppressor proteins. Taken together, these data suggest that a wide spectrum of cellular responses is induced to cope with chronic non-lethal toxic stresses in NCCIT cells.
authors.affiliation: Division of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do, Republic of Korea.
authors.forename: Nando Dulal
authors.initials: ND
authors.lastname: Das
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Hyung Tae
authors.initials: HT
authors.lastname: Lee
authors.forename: Kyoung Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Mi Ran
authors.initials: MR
authors.lastname: Choi
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D018053:Arsenites / Q000008:administration & dosage / Q000494:pharmacology*; D053898:Biosynthetic Pathways / Q000187:drug effects; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D058732:Embryoid Bodies / Q000187:drug effects; D054278:Embryonal Carcinoma Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D000091342:Endoplasmic Reticulum Chaperone BiP; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D014176:Protein Biosynthesis / Q000187:drug effects*; D017670:Sodium Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018053:Arsenites; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D017670:Sodium Compounds; C017947:sodium arsenite