title: Application of truncated nucleocapsid protein (N) for serotyping ELISA of murinae-associated hantavirus infection in rats.
abstract: Truncated recombinant nucleocapsid proteins (trNs) that lack N-terminally located cross-reactive epitopes of four Murinae rodent-associated hantaviruses, Seoul virus (SEOV), Thailand virus, Hantaan virus (HTNV) and Dobrava-Belgrade virus, were produced by using a baculovirus expression system. ELISA with the trNs as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses with cut-off OD values of 60% of those of whole N of HTNV. The trN-based ELISA could serotype 12 out of 13 sera obtained from wild rodents (Rattus norvegicus) naturally infected with SEOV using the 60% cut-off value. These results indicate that screening with whole N followed by serotyping with trNs using a cut-off OD value of 60% of that of whole N is a useful method for serological surveillance of Murinae-associated hantavirus infection among rodents.
authors.affiliation: Department of Microbiology, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060–8638, Japan.
authors.forename: Shumpei P
authors.initials: SP
authors.lastname: Yasuda
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koma
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimizu
authors.forename: Rika
authors.initials: R
authors.lastname: Endo
authors.forename: Rie
authors.initials: R
authors.lastname: Isozumi
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006476:Orthohantavirus / Q000145:classification* / Q000235:genetics; D018778:Hantavirus Infections / Q000175:diagnosis / Q000821:virology*; D007074:Immunoglobulin G / Q000032:analysis; D019590:Nucleocapsid Proteins / Q000737:chemistry* / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012703:Serotyping / Q000379:methods*
chemical_list: D007074:Immunoglobulin G; D019590:Nucleocapsid Proteins; D011994:Recombinant Proteins