title: Neuroprotective effects of neuroglobin after mechanical injury.
abstract: Neuroglobin (NGB) is a recently discovered globin, which is widely expressed in vertebrates central and peripheral nervous systems. Previous studies have shown that NGB is important in protecting neurons from hypoxic/ischemic brain injuries. However, there are no reports on the neuroprotective effects of NGB after mechanical injury. Currently, we showed that the NGB expression level in neurons increased continuously from 2 h after injury, and reached a peak at 16 h (p<0.01), after which it decreased sharply. NGB that was overexpressed in mechanically injured B104 cells showed significant neuroprotective effects. Lactate dehydrogenase (LDH) activity decreased and cell survival rates increased (p<0.01, n=5). In the rat model of focal brain trauma, the NGB expression increased sharply at 1 h, after which it increased continuously until it reached a peak at 6 h, and then gradually decreased (p<0.01, n=5). Furthermore, moderate and severe injury resulted in significantly higher NGB levels than did mild injury (p<0.01, n=5). Our results indicate that NGB exerts significant neuroprotective effects after mechanical injury, and thus has important implications for the prognosis and cure of traumatic brain injury.
authors.affiliation: Department of Neurosurgery, General Hospital of Chinese PLA, No. 28 Fuxing Road, Beijing, 100853, China.
authors.forename: Aijia
authors.initials: A
authors.lastname: Shang
authors.forename: Kaidong
authors.initials: K
authors.lastname: Liu
authors.forename: Hangyan
authors.initials: H
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xingyi
authors.initials: X
authors.lastname: Hang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zhang
authors.forename: Dingbiao
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001930:Brain Injuries / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000502:physiology; D005914:Globins / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000097:blood / Q000378:metabolism; D000078787:Neuroglobin; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000378:metabolism*; D010812:Physical Stimulation / Q000009:adverse effects; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014162:Transfection / Q000379:methods
chemical_list: D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D018696:Neuroprotective Agents; D016760:Proto-Oncogene Proteins c-fos; D005914:Globins; D007770:L-Lactate Dehydrogenase