title: Antinociceptive activity and acute toxicity study of Flaveria trinervia whole plant extracts using mice.
abstract: The antinociceptive activity of Flaveria trinervia extracts by tail-flicking and writhing methods was evaluated using mice. The abdominal writhing method was carried out by administering petroleum ether, chloroform and methanol extracts orally. After 1 h of incubation, 0.6% acetic acid (10 mL kg(-1)) was administered intraperitoneally. After 5 min, each group of mice was observed for the amount of writhing for a duration of 20 min. Acetyl salicylic acid was used as the standard reference. The tail-flick method was carried out using a thermal model and the maximum possible analgesia was calculated. The LD₅₀ values of petroleum ether, chloroform and methanol extracts were 700, 700 and 500 mg kg⁻¹, respectively. The methanol extract at 50 mg kg⁻¹ showed significant antinociceptive activity. The petroleum ether and chloroform extracts showed moderate antinociceptive activity at a dose of 70 mg kg⁻¹. The methanol extract showed more significant antinociceptive activity than the other extracts but was less effective than the standard. This investigation finds support for the ethnomedicinal claims of F. trinervia.
authors.affiliation: P.G. Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta 577451, Karnataka, India.
authors.forename: H Joy
authors.initials: HJ
authors.lastname: Hoskeri
authors.forename: V
authors.initials: V
authors.lastname: Krishna
authors.forename: P Suresh
authors.initials: PS
authors.lastname: Babu
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000473:Alkanes; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002725:Chloroform; D037142:Flaveria / Q000737:chemistry*; D007928:Lethal Dose 50; D000432:Methanol; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D010147:Pain Measurement; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D023382:Toxicity Tests, Acute
chemical_list: D000473:Alkanes; D000700:Analgesics; D010936:Plant Extracts; D002725:Chloroform; C004544:naphtha; D019342:Acetic Acid; D000432:Methanol