title: Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning.
abstract: The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development.
authors.affiliation: IRGS, Biogem, Ariano Irpino (AV), Italy.
authors.forename: Henrik
authors.initials: H
authors.lastname: Fagman
authors.forename: Elena
authors.initials: E
authors.lastname: Amendola
authors.forename: Luca
authors.initials: L
authors.lastname: Parrillo
authors.forename: Pietro
authors.initials: P
authors.lastname: Zoppoli
authors.forename: Pina
authors.initials: P
authors.lastname: Marotta
authors.forename: Marzia
authors.initials: M
authors.lastname: Scarfò
authors.forename: Pasquale
authors.initials: P
authors.lastname: De Luca
authors.forename: Denise Pires
authors.initials: DP
authors.lastname: de Carvalho
authors.forename: Michele
authors.initials: M
authors.lastname: Ceccarelli
authors.forename: Mario
authors.initials: M
authors.lastname: De Felice
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lauro
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D004064:Digestive System / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D061346:Laser Capture Microdissection; D008168:Lung / Q000196:embryology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D038081:Organogenesis / Q000235:genetics; D013961:Thyroid Gland / Q000196:embryology / Q000378:metabolism*; D013997:Time Factors; D059467:Transcriptome