title: Antinociceptive and anti-inflammatory properties of 7-epiclusianone, a prenylated benzophenone from Garcinia brasiliensis.
abstract: 7-Epiclusianone, a natural prenylated benzophenone, was extracted from Garcinia brasiliensis Planch. & Triana (Clusiaceae), a native plant commonly known as bacupari and used in traditional Brazilian medicine for the treatment of inflammatory diseases. As a result of the wide spectrum of biological activities attributed to polyisoprenylated benzophenones, the aim of this study was to evaluate the analgesic and anti-inflammatory effects of 7-epiclusianone using two animal models. Carrageenan-induced paw oedema and peritonitis were used to investigate the anti-inflammatory activity of 7-epiclusianone in rats. The acetic acid-induced writhing, formalin and hot-plate tests were used to investigate its antinociceptive activity in mice. At test doses of 5, 10 and 15 mg/kg p.o., 7-epiclusianone had an anti-inflammatory effect as demonstrated by the reduction of paw oedema induced by carrageenan and the inhibition of leukocyte recruitment into the peritoneal cavity. At the same doses, 7-epiclusianone inhibited nociception induced by an intraperitoneal injection of acetic acid, observed by the decrease in the number of writhing episodes. Additionally, 7-epiclusianone decreased licking time caused by a subplantar injection of formalin. Moreover, the hot plate test produced a significant increase in latency reaction, demonstrating an antinociceptive effect. The experimental data demonstrated that the polyisoprenylated benzophenone 7-epiclusianone has remarkable anti-inflammatory and antinociceptive activities.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas-MG, 37130-000 Alfenas, Brazil.
authors.forename: Flávia V
authors.initials: FV
authors.lastname: Santa-Cecília
authors.forename: Lissara A S
authors.initials: LA
authors.lastname: Freitas
authors.forename: Fabiana C
authors.initials: FC
authors.lastname: Vilela
authors.forename: Clarice de C
authors.initials: Cde C
authors.lastname: Veloso
authors.forename: Cláudia Q
authors.initials: CQ
authors.lastname: da Rocha
authors.forename: Maria E C
authors.initials: ME
authors.lastname: Moreira
authors.forename: Danielle F
authors.initials: DF
authors.lastname: Dias
authors.forename: Alexandre
authors.initials: A
authors.lastname: Giusti-Paiva
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: dos Santos
mesh_terms: D019342:Acetic Acid / Q000009:adverse effects; D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001522:Behavior, Animal / Q000187:drug effects; D001577:Benzophenones / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D016227:Benzoquinones / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan / Q000009:adverse effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D029760:Garcinia / Q000737:chemistry*; D006358:Hot Temperature / Q000009:adverse effects; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D051379:Mice; D010147:Pain Measurement; D010538:Peritonitis / Q000139:chemically induced / Q000188:drug therapy; D054876:Prenylation; D051381:Rats
chemical_list: C118110:7-epiclusianone; D000700:Analgesics; D000893:Anti-Inflammatory Agents; D001577:Benzophenones; D016227:Benzoquinones; D008070:Lipopolysaccharides; D002351:Carrageenan; D019342:Acetic Acid