title: Mapping open chromatin with formaldehyde-assisted isolation of regulatory elements.
abstract: Noncoding regulatory genomic elements are central for cellular function, differentiation, and disease, but remain poorly characterized. FAIRE (formaldehyde-assisted isolation of regulatory elements) has emerged as a simple method to identify and analyze active regulatory sequences based on their decreased nucleosomal content. More recently FAIRE was combined with high-throughput sequencing (FAIRE-seq) to locate tissue-specific regulatory elements at a genome scale in purified human pancreatic islets. Here we describe the implementation of the FAIRE method in human pancreatic islet cells.
authors.affiliation: Genomic Programming of Beta Cells, Institut d'Investigacions Biomediques August Pi i Sunyer, Barcelona, Spain.
authors.forename: Takao
authors.initials: T
authors.lastname: Nammo
authors.forename: Santiago A
authors.initials: SA
authors.lastname: Rodríguez-Seguí
authors.forename: Jorge
authors.initials: J
authors.lastname: Ferrer
mesh_terms: D002460:Cell Line; D005591:Chemical Fractionation; D002725:Chloroform / Q000737:chemistry; D002843:Chromatin / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005557:Formaldehyde / Q000378:metabolism*; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology; D019800:Phenol / Q000737:chemistry; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013010:Sonication; D046509:Tissue Culture Techniques; D016707:Tissue Fixation
chemical_list: D002843:Chromatin; D005557:Formaldehyde; D019800:Phenol; D002725:Chloroform; D004247:DNA