title: Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected with Trypanosoma cruzi.
abstract: This study aimed to evaluate the activity of cholinesterases and adenosine deaminase (ADA) in blood and serum of rats infected with Trypanosoma cruzi. Twelve adult rats were used in the experiment divided into two uniform groups. Rodents from group A (control group) were non-infected and animals from group B served as infected, receiving intraperitoneally 3·3×10(7) trypomastigotes/each. Blood collection was performed at days 60 and 120 post-infection (PI) in order to evaluate the hemogram, blood activity of acetylcholinesterase, and serum butyrylcholinesterase and ADA activities. Hematological parameters did not differ between groups. A significant increase (P<0·05) of acetylcholinesterase activity was observed in blood while butyrylcholinesterase had a significant reduction (P<0·01) in serum of infected rats at days 60 and 120 PI. ADA activity in serum showed an inhibition in infected animals when compared to non-infected at day 120 PI. Based on these results, it is possible to conclude that the activity of cholinesterases and ADA were changed in animals infected with T. cruzi. The possible causes of these alterations will be discussed in this paper.
authors.affiliation: Universidade Federal de Santa Maria, Brazil. aleksandro_ss@yahoo.com.br
authors.forename: A S
authors.initials: AS
authors.lastname: Da Silva
authors.forename: V C
authors.initials: VC
authors.lastname: Pimentel
authors.forename: A M
authors.initials: AM
authors.lastname: Fiorenza
authors.forename: R T
authors.initials: RT
authors.lastname: França
authors.forename: A A
authors.initials: AA
authors.lastname: Tonin
authors.forename: J A
authors.initials: JA
authors.lastname: Jaques
authors.forename: C A M
authors.initials: CA
authors.lastname: Leal
authors.forename: C B
authors.initials: CB
authors.lastname: Da Silva
authors.forename: V
authors.initials: V
authors.lastname: Morsch
authors.forename: M R C
authors.initials: MR
authors.lastname: Sschetinger
authors.forename: S T A
authors.initials: ST
authors.lastname: Lopes
authors.forename: S G
authors.initials: SG
authors.lastname: Monteiro
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood*; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D014355:Chagas Disease / Q000201:enzymology / Q000276:immunology*; D002802:Cholinesterases / Q000097:blood*; D006321:Heart / Q000469:parasitology*; D008297:Male; D009043:Motor Activity; D051381:Rats; D014349:Trypanosoma cruzi / Q000472:pathogenicity*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D002802:Cholinesterases; D000243:Adenosine Deaminase