title: Quantitative determination of lisinopril in human plasma by high performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.
abstract: A rapid, selective and sensitive high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed to determine lisinopril in human plasma. Sample pretreatment involved a one-step protein precipitation with methanol of 0.1 mL plasma. Analysis was performed on an Inertsil ODS-3 column (2.1 × 50 mm i.d., 3 µm) with mobile phase consisting of methanol-water (containing 0.2% formic acid; 55:45, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode via an electrospray ionization source. Each plasma sample was chromatographed within 2.5 min. The linear calibration curves for lisinopril were obtained in the concentration range of 1.03-206 ng/mL (r(2)  ≥ 0.99) with a lower limit of quantification of 1.03 ng/mL. The intra- and inter-day precisions (relative standard deviation) were not higher than 11%, and accuracy (relative error) was within ±6.8%, determined from quality control samples for lisinopril, which corresponded to the guidance of the Food and Drug Administration. The method described herein was fully validated and successfully applied to the pharmacokinetic study of lisinopril tablets in healthy male volunteers after oral administration.
authors.affiliation: Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Shuyan
authors.initials: S
authors.lastname: Yang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jing
authors.forename: Zhili
authors.initials: Z
authors.lastname: Xiong
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015773:Enalaprilat; D006801:Humans; D016014:Linear Models; D017706:Lisinopril / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017706:Lisinopril; D015773:Enalaprilat