title: Bacterial community dynamics in aerated cow manure slurry at different aeration intensities.
abstract: OBJECTIVE
  This study aimed to characterize microbial community dynamics in aerated cow manure slurry at different aeration intensities.
  
  
  RESULTS
  Batch aerobic treatments were set up in 5-l jar fermentor, each containing 3 l of manure slurry; the slurries were subjected to low, medium and high (50, 150 and 250 ml min(-1), respectively) aeration for 9 days. Microbial community composition was determined using terminal restriction fragment length polymorphism and a clone library targeting 16S rRNA genes. High and medium aeration accelerated organic carbon degradation in parallel with the degree of aeration intensity; however, 90% of the initial total organic carbon was retained during low-aeration treatment. During the active stages of organic carbon decomposition, clones belonging to the class Bacilli accumulated. Moreover, Bacilli accumulation occurred earlier under high aeration than under medium aeration.
  
  
  CONCLUSIONS
  Organic matter degradation was mainly governed by a common microbial assemblage consisting of many lineages belonging to the class Bacilli. The timing of community development differed depending on aeration intensity.
  
  
  CONCLUSIONS
  This study reports on changes in several environmentally important parameters and the principal microbial assemblage during the pollution-reducing phase of cattle manure aeration treatment.
authors.affiliation: Waste Recycling Research Team, National Agricultural Research Center for Hokkaido Region, Sapporo, Hokkaido, Japan. hanadi@affrc.go.jp
authors.forename: D
authors.initials: D
authors.lastname: Hanajima
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumoto
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Maeda
authors.forename: R
authors.initials: R
authors.lastname: Morioka
mesh_terms: D000332:Aerobiosis; D000818:Animals; D001419:Bacteria / Q000235:genetics / Q000254:growth & development*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D002244:Carbon / Q000378:metabolism*; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D015723:Gene Library; D008372:Manure / Q000382:microbiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D008372:Manure; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002244:Carbon