title: [Establishment of palatal organ culture in vitro].
abstract: OBJECTIVE
  The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro.
  
  
  METHODS
  20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin.
  
  
  RESULTS
  All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused.
  
  
  CONCLUSIONS
  This method provides an effective way for investigating the etiology of cleft palate in vitro.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Bing
authors.initials: B
authors.lastname: Shi
authors.forename: Tian
authors.initials: T
authors.lastname: Meng
authors.forename: Chenghao
authors.initials: C
authors.lastname: Li
authors.forename: Xinghua
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D002972:Cleft Palate; D004848:Epithelium; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D009924:Organ Culture Techniques; D010159:Palate / Q000166:cytology*; D011247:Pregnancy