title: [Antiviral effect of lamivudine on HIV-1 targeting MT2 cells influenced by morphine].
abstract: OBJECTIVE
  To determine whether morphine having the ability to influence the antiviral effect of lamivudine (3TC) in vitro study.
  
  
  METHODS
  MT2 cells were randomly assigned into morphine + 3TC treatment group, morphine + naloxone + 3TC treatment group, naloxone + 3TC treatment group. Both 3TC and virus control groups were set up. The corresponding MT2 cells were treated with opiates antagonist (naloxone) for 0.5 hours before the 24-hours morphine treatment program was implemented while all of the groups were then infected with equal amounts of cell-free HIV-1 IIIB strain and 3TC. HIV-1 p24 antigen in culture supernatants collected at days 3, 4, 5 and 6 after infection status was tested and the inhibition of 3TC anti-HIV-1 p24 antigen of various treatment groups calculated.
  
  
  RESULTS
  Inhibition of 3TC anti-HIV-1 p24 antigen of Morphine + 3TC treatment group was the lowest when HIV-1 infected cells at 3(rd)and 4(th) day and showed significant difference (P < 0.05) when compared to the 3TC control. However, there was no statistically significant difference among them (P > 0.05), when virus was infected the cells at 5(th)and 6(th) day. The difference of 3TC anti-HIV-1 p24 antigen inhibition between the morphine + naloxone + 3TC treatment group and the naloxone + 3TC treatment group was not significant (P > 0.05). Similar results were obtained when these two groups were compared to the 3TC control group (P > 0.05), respectively. The 3TC anti-HIV-1 p24 antigen inhibition of each treatment group reduced as the time of infection prolonged, showing a significant and time-course effect.
  
  
  CONCLUSIONS
  The 3TC antiviral effect was reduced by morphine in the early stage of infection, and could be blocked by naloxone.
authors.affiliation: Guangxi Key Laboratory Cultivation Base of AIDS Prevention and Treatment & Department of Epidemiology of School of Public Health, Guangxi Medical University, Nanning 530021, China.
authors.forename: Bing-yu
authors.initials: BY
authors.lastname: Liang
authors.forename: Dao-min
authors.initials: DM
authors.lastname: Zhuang
authors.forename: Jun-jun
authors.initials: JJ
authors.lastname: Jiang
authors.forename: Si-yang
authors.initials: SY
authors.lastname: Liu
authors.forename: Qi-jian
authors.initials: QJ
authors.lastname: Su
authors.forename: Jing-yun
authors.initials: JY
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D002460:Cell Line; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology*; D009020:Morphine / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D019259:Lamivudine; D009270:Naloxone; D009020:Morphine