title: Effects of age and hypertension on α1-adrenoceptors in the major source arteries of the rat bladder and penis.
abstract: α(1)-Adrenoceptors regulate blood pressure, regional vascular resistance and tissue blood flow. As aging and hypertension may impact pelvic arterial blood flow resulting in bladder and penile dysfunction, we investigated effects of age and hypertension on α(1)-adrenoceptors in the major source arteries of the rat bladder and penis. Using radioligand receptor binding, real-time reverse transcription-polymerase chain reaction (RT-PCR) and fluorescent microsphere infusion techniques, we compared 3 and 22-month-old male Fischer rats, and male normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). Twenty-two-month-old rats and SHRs had significantly higher total α(1)-adrenoceptor density in the internal iliac artery and lower blood flow to the bladder and penis than 3-month-old and WKY rats, respectively. RT-PCR data showed an age and hypertension related increase in the expression of α(1B)-adrenoceptor mRNA in the internal iliac, vesical and internal pudendal arteries and a switch from α(1A) predominance in 3-month-old and WKY rats to α(1B)>α(1A) in 22-month-old rats and SHRs. Our data indicate the presence of age and hypertension related alterations in vascular α(1)-adrenoceptor subtype distribution and in blood flow to the rat bladder and penis. These findings suggest that pharmacological blockade of the vascular α(1B)-adrenoceptor, which could increase pelvic blood flow, may contribute to the improvement of bladder and penile dysfunctions in animal models for aging and hypertension.
authors.affiliation: Department of Clinical Pharmacology, Nishi-Kumamoto Hospital, Medical Co. LTA, 1012 Koga, Tomiai-machi, Kumamoto 861-4157, Japan. yonomakoto@hotmail.com
authors.forename: Makoto
authors.initials: M
authors.lastname: Yono
authors.forename: Takanori
authors.initials: T
authors.lastname: Tanaka
authors.forename: Shigeki
authors.initials: S
authors.lastname: Tsuji
authors.forename: Shin
authors.initials: S
authors.lastname: Irie
authors.forename: Yukikuni
authors.initials: Y
authors.lastname: Sakata
authors.forename: Masayuki
authors.initials: M
authors.lastname: Otani
authors.forename: Masaki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Jamshid
authors.initials: J
authors.lastname: Latifpour
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001158:Arteries / Q000378:metabolism* / Q000502:physiology / Q000503:physiopathology; D001775:Blood Circulation; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D010413:Penis / Q000098:blood supply*; D051381:Rats; D018340:Receptors, Adrenergic, alpha-1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001743:Urinary Bladder / Q000098:blood supply*
chemical_list: D018340:Receptors, Adrenergic, alpha-1