title: Recognition of forcible curvature in circular DNA by human Topoisomerase I.
abstract: A series of forcible curved circular DNAs (cDNAs) were prepared to investigate the recognition features of human Topoisomerase I (hTopo I). The IC(50) can be modulated by the curvature degrees of cDNA. In addition, preferential bindings of hTopo I to cDNA with high curvature have been observed by AFM and EMSA.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
authors.forename: Zhaoqi
authors.initials: Z
authors.lastname: Yang
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.forename: Shuhui
authors.initials: S
authors.lastname: Hiew
authors.forename: Magdeline Taotao
authors.initials: MT
authors.lastname: Ng
authors.forename: Weixing
authors.initials: W
authors.lastname: Yuan
authors.forename: Haibin
authors.initials: H
authors.lastname: Su
authors.forename: Fangwei
authors.initials: F
authors.lastname: Shao
authors.forename: Tianhu
authors.initials: T
authors.lastname: Li
mesh_terms: D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004270:DNA, Circular / Q000737:chemistry* / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D006801:Humans; D018625:Microscopy, Atomic Force; D011485:Protein Binding
chemical_list: D004270:DNA, Circular; D004264:DNA Topoisomerases, Type I