title: Isolation of Salmonella typhi from apparently healthy liver.
abstract: It is suggested that Salmonella typhi resides mostly in hepatobiliary system especially in gallbladder in chronic typhoid carriers. It is not very clear whether in gallbladder lumen or on its wall or in liver. However, we had been successful in detecting S. typhi in liver by PCR targeting flagellin gene sequences. Therefore, in the present study, we tried to isolate the bacterium from liver tissue collected from dead bodies brought for post mortem examination. We could isolate S. typhi in 2 of 20 such liver tissues examined by using conventional isolation techniques. The isolates were identified by routine phenotypic characters and were confirmed by amplification and sequencing of two conserved genes i.e. 16S rDNA and flagellin (fliC) gene followed by blasting on www.ncbi.nlm.nih.gov.
authors.affiliation: Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. gopalnath@gmail.com
authors.forename: Gopal
authors.initials: G
authors.lastname: Nath
authors.forename: Chandra Bhan
authors.initials: CB
authors.lastname: Pratap
authors.forename: Saurabh K
authors.initials: SK
authors.lastname: Patel
authors.forename: Anil K
authors.initials: AK
authors.lastname: Gulati
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Tripathi
mesh_terms: D001483:Base Sequence; D002102:Cadaver; D002353:Carrier State; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005408:Flagellin / Q000235:genetics; D006801:Humans; D008099:Liver / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012485:Salmonella typhi / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005408:Flagellin