title: The role of reactive oxygen species scavenging enzymes in the development of septic loosening after total hip replacement.
abstract: In this study of 41 patients, we used proteomic, Western blot and immunohistochemical analyses to show that several reactive oxygen species scavenging enzymes are expressed differentially in patients with primary osteoarthritis and those with non-loosening and aseptic loosening after total hip replacement (THR). The patients were grouped as A (n = 16, primary THR), B (n = 10, fixed THR but requiring revision for polyethylene wear) and C (n = 15, requiring revision due to aseptic loosening) to verify the involvement of the identified targets in aseptic loosening. When compared with Groups A and B, Group C patients exhibited significant up-regulation of transthyretin and superoxide dismutase 3, but down-regulation of glutathione peroxidase 2 in their hip synovial fluids. Also, higher levels of superoxide dismutase 2 and peroxiredoxin 2, but not superoxide dismutase 1, catalase and glutathione perioxidase 1, were consistently detected in the hip capsules of Group C patients. We propose that dysregulated reactive oxygen species-related enzymes may play an important role in the pathogenesis and progression of aseptic loosening after THR.
authors.affiliation: Division of Sports Medicine, Department of Orthopaedic Surgery, Chang-Gung Memorial Hospital, 6 West, Chia-Pu Road, Puzih City, Chiayi 613, Taiwan.
authors.forename: K-T
authors.initials: KT
authors.lastname: Peng
authors.forename: W-H
authors.initials: WH
authors.lastname: Hsu
authors.forename: H-N
authors.initials: HN
authors.lastname: Shih
authors.forename: C-W
authors.initials: CW
authors.lastname: Hsieh
authors.forename: T-W
authors.initials: TW
authors.lastname: Huang
authors.forename: R W-W
authors.initials: RW
authors.lastname: Hsu
authors.forename: P-J
authors.initials: PJ
authors.lastname: Chang
mesh_terms: D000368:Aged; D019644:Arthroplasty, Replacement, Hip / Q000009:adverse effects*; D015153:Blotting, Western; D015536:Down-Regulation; D004798:Enzymes / Q000378:metabolism; D005260:Female; D016166:Free Radical Scavengers / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000201:enzymology* / Q000601:surgery; D011475:Prosthesis Failure / Q000009:adverse effects*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013582:Synovial Fluid / Q000737:chemistry*; D015854:Up-Regulation
chemical_list: D004798:Enzymes; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species