title: Activity in vivo of anti-Trypanosoma cruzi compounds selected from a high throughput screening.
abstract: Novel technologies that include recombinant pathogens and rapid detection methods are contributing to the development of drugs for neglected diseases. Recently, the results from the first high throughput screening (HTS) to test compounds for activity against Trypanosoma cruzi trypomastigote infection of host cells were reported. We have selected 23 compounds from the hits of this HTS, which were reported to have high anti-trypanosomal activity and low toxicity to host cells. These compounds were highly purified and their structures confirmed by HPLC/mass spectrometry. The compounds were tested in vitro, where about half of them confirmed the anti-T. cruzi activity reported in the HTS, with IC50 values lower than 5 µM. We have also adapted a rapid assay to test anti-T. cruzi compounds in vivo using mice infected with transgenic T. cruzi expressing luciferase as a model for acute infection. The compounds that were active in vitro were also tested in vivo using this assay, where we found two related compounds with a similar structure and low in vitro IC50 values (0.11 and 0.07 µM) that reduce T. cruzi infection in the mouse model more than 90% after five days of treatment. Our findings evidence the benefits of novel technologies, such as HTS, for the drug discovery pathway of neglected diseases, but also caution about the need to confirm the results in vitro. We also show how rapid methods of in vivo screening based in luciferase-expressing parasites can be very useful to prioritize compounds early in the chain of development.
authors.affiliation: Division of Medical Parasitology, Department of Microbiology, New York University School of Medicine, United States of America.
authors.forename: Grasiella
authors.initials: G
authors.lastname: Andriani
authors.forename: Anne-Danielle C
authors.initials: AD
authors.lastname: Chessler
authors.forename: Gilles
authors.initials: G
authors.lastname: Courtemanche
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Burleigh
authors.forename: Ana
authors.initials: A
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001681:Biological Assay / Q000379:methods; D014355:Chagas Disease / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid; D004195:Disease Models, Animal; D057166:High-Throughput Screening Assays; D020128:Inhibitory Concentration 50; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D021261:Parasitic Sensitivity Tests; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents