title: Quinolone induction of qnrVS1 in Vibrio splendidus and plasmid-carried qnrS1 in Escherichia coli, a mechanism independent of the SOS system.
abstract: Plasmid-carried qnrS1 is derived from Vibrio splendidus chromosomal qnrVS1. qnrVS1 transcripts increased 21- to 34-fold with subinhibitory concentrations of ciprofloxacin but much less with mitomycin. No LexA binding sites were upstream of qnrS1 or qnrVS1, and similar induction levels were observed in lexA-positive and lexA-negative Escherichia coli strains with native qnrS1 plasmid pMG306 but not with pUC18-cloned qnrS1 or qnrVS1. Thus, qnrS1 induction by quinolones is independent of the SOS system and requires sequence besides that of the structural gene.
authors.affiliation: Division of Infectious Diseases, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114-2696, USA.
authors.forename: Ryo
authors.initials: R
authors.lastname: Okumura
authors.forename: Chun-Hsing
authors.initials: CH
authors.lastname: Liao
authors.forename: Mariah
authors.initials: M
authors.lastname: Gavin
authors.forename: George A
authors.initials: GA
authors.lastname: Jacoby
authors.forename: David C
authors.initials: DC
authors.lastname: Hooper
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects; D029968:Escherichia coli Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008826:Microbial Sensitivity Tests; D016685:Mitomycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D015363:Quinolones / Q000494:pharmacology*; D013014:SOS Response, Genetics / Q000187:drug effects*; D014733:Vibrio / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D015363:Quinolones; D016685:Mitomycin; D002939:Ciprofloxacin