title: [Expression of glucose transporter-1 and insulin receptor alpha1 in osteoblast obtained from diabetic rats' mandibles].
abstract: OBJECTIVE
  To explore the expression of glucose transporter (GLUT)-1 and insulin receptor (IR) alpha1 in osteoblast obtained from diabetic rats' mandibles.
  
  
  METHODS
  The expression of GLUT-1 and IR alpha1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain.
  
  
  RESULTS
  The mRNA expressions of GLUT-1 and IR alpha1 of diabetic group were significantly higher than control group (P<0.05). The protein expressions of GLUT-1 and IR alpha1 were similar to the mRNA expressions.
  
  
  CONCLUSIONS
  Osteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.
authors.affiliation: Dept. of Prosthodontics, Stomatology Hospital of Dalian City, Dalian 116021, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wu
authors.forename: Hongchen
authors.initials: H
authors.lastname: Liu
authors.forename: Lingling
authors.initials: L
authors.lastname: E
authors.forename: Zhenchun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D051246:Glucose Transport Proteins, Facilitative; D008334:Mandible; D010006:Osteoblasts; D051381:Rats; D011972:Receptor, Insulin
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D011972:Receptor, Insulin