title: Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii Standl.
abstract: The crystal structure and pro-inflammatory property of a lectin from the seeds of Dioclea wilsonii (DwL) were analyzed to gain a better understanding of structure/function relationships of Diocleinae lectins. Following crystallization and structural determination by standard molecular replacement techniques, DwL was found to be a tetramer based on PISA analysis, and composed by two metal-binding sites per monomer and loops which are involved in molecular oligomerization. DwL presents 96% and 99% identity with two other previously described lectins of Dioclea rostrata (DRL) and Dioclea grandiflora (DGL). DwL differs structurally from DVL and DRL with regard to the conformation of the carbohydrate recognition domain and related biological activities. The structural analysis of DwL in comparison to other Diocleinae lectins can be related to the differences in the dose-dependent pro-inflammatory effect elicited in Wistar rats, probably via specific interactions with mast cells complex carbohydrate, resulting in significant paw edema. DwL appears to be involved in positive modulation of mast cell degranulation via recognition of surface carbohydrates. Since this recognition is dependent on site volume and CRD configuration, edematogenesis mediated by resident cells varies in potency and efficacy among different Diocleinae lectins.
authors.affiliation: Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Brazil.
authors.forename: Thaiz Batista Azevedo
authors.initials: TB
authors.lastname: Rangel
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: Rocha
authors.forename: Gustavo Arruda
authors.initials: GA
authors.lastname: Bezerra
authors.forename: Ana Maria Sampaio
authors.initials: AM
authors.lastname: Assreuy
authors.forename: Alana de Freitas
authors.initials: Ade F
authors.lastname: Pires
authors.forename: Antonia Sâmia Fernandes
authors.initials: AS
authors.lastname: do Nascimento
authors.forename: Maria Julia Barbosa
authors.initials: MJ
authors.lastname: Bezerra
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Karl
authors.initials: K
authors.lastname: Gruber
authors.forename: Plínio
authors.initials: P
authors.lastname: Delatorre
authors.forename: Patrícia Machado Bueno
authors.initials: PM
authors.lastname: Fernandes
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000818:Animals; D001665:Binding Sites; D015550:Cell Degranulation / Q000187:drug effects* / Q000276:immunology; D018360:Crystallography, X-Ray; D052598:Dioclea / Q000737:chemistry*; D004306:Dose-Response Relationship, Immunologic; D004487:Edema / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D006614:Hindlimb; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008958:Models, Molecular; D009929:Organ Size / Q000187:drug effects; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding; D055503:Protein Multimerization; D017434:Protein Structure, Tertiary; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics
chemical_list: D037121:Plant Lectins