title: The fluorescence spectroscopic study on the interaction between imidazo[2,1-b]thiazole analogues and bovine serum albumin.
abstract: The interaction between imidazo[2,1-b]thiazole (IMTZ) and bovine serum albumin (BSA) was analyzed by fluorescence and ultraviolet spectroscopy at 302 and 310 K under simulative physiological conditions. The results show that IMTZ can effectively quench the intrinsic fluorescence of BSA via static and dynamic quenching. The binding constant, binding sites of IMTZ with BSA were calculated. According to the Förster non-radiation energy transfer theory, the average binding distance between IMTZ and BSA was obtained. What's more, the synchronous fluorescence spectra indicated that the conformation of BSA has been changed. The results provided the information for the binding of IMTZ to BSA, and the influences of substituent group on the interaction were also discussed.
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Lu
authors.initials: L
authors.lastname: Shiyu
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.forename: Liu
authors.initials: L
authors.lastname: Heting
authors.forename: Li
authors.initials: L
authors.lastname: Xiaofang
authors.forename: Yi
authors.initials: Y
authors.lastname: Pinggui
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D007093:Imidazoles / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D007093:Imidazoles; D013844:Thiazoles; D012710:Serum Albumin, Bovine