title: When multiphoton microscopy sees near infrared.
abstract: The need for quantification and real time visualization of developmental processes has called for increasingly sophisticated imaging techniques. Among them, multiphoton microscopy reveals itself to be an extremely versatile tool owing to its unique ability to combine fluorescent imaging, laser ablation, and higher harmonic generation. Furthermore, recent advances in femtosecond lasers and optical parametric oscillators (OPO) are now opening doors for imaging at unprecedented wavelengths centered in the tissue transparency window. This Review describes promising multiphoton approaches using OPO and the growing number of useful applications of non-linear microscopy in the field of developmental biology. Basic characteristics associated with these techniques are described along with the main experimental challenges when applied to embryo imaging.
authors.affiliation: IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), Inserm U964, CNRS UMR7104, Université de Strasbourg, 1 rue Laurent Fries, Illkirch F-67404, France.
authors.forename: Halina
authors.initials: H
authors.lastname: Mojzisova
authors.forename: Julien
authors.initials: J
authors.lastname: Vermot
mesh_terms: D000818:Animals; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods*; D017711:Nonlinear Dynamics; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation / Q000379:methods*