title: CpG island methylation of microRNAs is associated with tumor size and recurrence of non-small-cell lung cancer.
abstract: We investigated whether the CpG island methylation of certain microRNAs was associated with the clinicopathological features and the prognosis of non-small-cell lung cancer. The methylation of mir-152, -9-3, -124-1, -124-2, and -124-3 was analyzed in 96 non-small-cell lung cancer specimens using a combined bisulfite restriction analysis. The median observation period was 49.5 months. The methylation of mir-9-3, -124-2, and -124-3 was individually associated with an advanced T factor independent of age, sex, and smoking habit. Moreover, the methylation of multiple microRNA loci was associated with a poorer progression-free survival in a univariate analysis. Our result enlightens the accumulation of aberrant DNA methylation which occurs in concordance with the tumor progression.
authors.affiliation: Department of Thoracic Surgery, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kitano
authors.forename: Kousuke
authors.initials: K
authors.lastname: Watanabe
authors.forename: Noriko
authors.initials: N
authors.lastname: Emoto
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kage
authors.forename: Emi
authors.initials: E
authors.lastname: Hamano
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagase
authors.forename: Atsushi
authors.initials: A
authors.lastname: Sano
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Murakawa
authors.forename: Jun
authors.initials: J
authors.lastname: Nakajima
authors.forename: Akiteru
authors.initials: A
authors.lastname: Goto
authors.forename: Masashi
authors.initials: M
authors.lastname: Fukayama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
authors.forename: Nobuya
authors.initials: N
authors.lastname: Ohishi
authors.forename: Daiya
authors.initials: D
authors.lastname: Takai
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D018899:CpG Islands; D019175:DNA Methylation; D018450:Disease Progression; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local
chemical_list: D035683:MicroRNAs