title: Novel aptamer-nanoparticle bioconjugates enhances delivery of anticancer drug to MUC1-positive cancer cells in vitro.
abstract: MUC1 protein is an attractive target for anticancer drug delivery owing to its overexpression in most adenocarcinomas. In this study, a reported MUC1 protein aptamer is exploited as the targeting agent of a nanoparticle-based drug delivery system. Paclitaxel (PTX) loaded poly (lactic-co-glycolic-acid) (PLGA) nanoparticles were formulated by an emulsion/evaporation method, and MUC1 aptamers (Apt) were conjugated to the particle surface through a DNA spacer. The aptamer conjugated nanoparticles (Apt-NPs) are about 225.3 nm in size with a stable in vitro drug release profile. Using MCF-7 breast cancer cell as a MUC1-overexpressing model, the MUC1 aptamer increased the uptake of nanoparticles into the target cells as measured by flow cytometry. Moreover, the PTX loaded Apt-NPs enhanced in vitro drug delivery and cytotoxicity to MUC1(+) cancer cells, as compared with non-targeted nanoparticles that lack the MUC1 aptamer (P<0.01). The behavior of this novel aptamer-nanoparticle bioconjugates suggests that MUC1 aptamers may have application potential in targeted drug delivery towards MUC1-overexpressing tumors.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Yu
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Jinhong
authors.initials: J
authors.lastname: Duan
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xu
authors.forename: Xian-Da
authors.initials: XD
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D018396:Mucin-1 / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D017239:Paclitaxel / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D004337:Drug Carriers; C060192:MUC1 protein, human; D018396:Mucin-1; D017239:Paclitaxel