title: The Xanthomonas type III effector XopD targets the Arabidopsis transcription factor MYB30 to suppress plant defense.
abstract: Plant and animal pathogens inject type III effectors (T3Es) into host cells to suppress host immunity and promote successful infection. XopD, a T3E from Xanthomonas campestris pv vesicatoria, has been proposed to promote bacterial growth by targeting plant transcription factors and/or regulators. Here, we show that XopD from the B100 strain of X. campestris pv campestris is able to target MYB30, a transcription factor that positively regulates Arabidopsis thaliana defense and associated cell death responses to bacteria through transcriptional activation of genes related to very-long-chain fatty acid (VLCFA) metabolism. XopD specifically interacts with MYB30, resulting in inhibition of the transcriptional activation of MYB30 VLCFA-related target genes and suppression of Arabidopsis defense. The helix-loop-helix domain of XopD is necessary and sufficient to mediate these effects. These results illustrate an original strategy developed by Xanthomonas to subvert plant defense and promote development of disease.
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, Castanet-Tolosan, France.
authors.forename: Joanne
authors.initials: J
authors.lastname: Canonne
authors.forename: Daniel
authors.initials: D
authors.lastname: Marino
authors.forename: Alain
authors.initials: A
authors.lastname: Jauneau
authors.forename: Cécile
authors.initials: C
authors.lastname: Pouzet
authors.forename: Christian
authors.initials: C
authors.lastname: Brière
authors.forename: Dominique
authors.initials: D
authors.lastname: Roby
authors.forename: Susana
authors.initials: S
authors.lastname: Rivas
mesh_terms: D017360:Arabidopsis / Q000276:immunology* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D018257:Helix-Loop-Helix Motifs; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D057865:Plant Immunity; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000378:metabolism*; D014774:Virulence; D016959:Xanthomonas campestris / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; C464659:MYB30 protein, Arabidopsis; D014157:Transcription Factors