title: Phosphorylcholine Phosphatase: A Peculiar Enzyme of Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa synthesizes phosphorylcholine phosphatase (PchP) when grown on choline, betaine, dimethylglycine or carnitine. In the presence of Mg(2+) or Zn(2+), PchP catalyzes the hydrolysis of p-nitrophenylphosphate (p-NPP) or phosphorylcholine (Pcho). The regulation of pchP gene expression is under the control of GbdR and NtrC; dimethylglycine is likely the metabolite directly involved in the induction of PchP. Therefore, the regulation of choline metabolism and consequently PchP synthesis may reflect an adaptive response of P. aeruginosa to environmental conditions. Bioinformatic and biochemistry studies shown that PchP contains two sites for alkylammonium compounds (AACs): one in the catalytic site near the metal ion-phosphoester pocket, and another in an inhibitory site responsible for the binding of the alkylammonium moiety. Both sites could be close to each other and interact through the residues (42)E, (43)E and (82)YYY(84). Zn(2+) is better activator than Mg(2+) at pH 5.0 and it is more effective at alleviating the inhibition produced by the entry of Pcho or different AACs in the inhibitory site. We postulate that Zn(2+) induces at pH 5.0 a conformational change in the active center that is communicated to the inhibitory site, producing a compact or closed structure. However, at pH 7.4, this effect is not observed because to the hydrolysis of the [Zn(2+)L(2) (-1)L(2) (0)(H(2)O)(2)] complex, which causes a change from octahedral to tetrahedral in the metal coordination geometry. This enzyme is also present in P. fluorescens, P. putida, P. syringae, and other organisms. We have recently crystallized PchP and solved its structure.
authors.affiliation: Departamento de Biología Molecular, Área Bioquímica, Facultad de Ciencias Exactas, Fisicoquímicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Domenech
authors.forename: Lisandro Horacio
authors.initials: LH
authors.lastname: Otero
authors.forename: Paola Rita
authors.initials: PR
authors.lastname: Beassoni
authors.forename: Angela Teresita
authors.initials: AT
authors.lastname: Lisa