title: Proteomic analysis of human umbilical vein endothelial cells incubated with Cryptococcus neoformans var. neoformans.
abstract: Cryptococcus neoformans is a medically important fungus and can infect all the organs of the body. As vascular endothelial cell is an important target for C. neoformans to penetrate any organs, the differential protein expression of human umbilical vascular endothelial cell (HUVEC) after incubating with C. neoformans may be the key to penetration. The proteins of HUVECs incubated with C. neoformans and normal HUVECs were collected and purified. After two-dimensional electrophoresis, the differential protein expression was identified by matrix-assisted laser desorption/ionisation mass spectrometry. The mRNA levels of some proteins were measured by real-time PCR. Three proteins were found significantly overexpressed in HUVECs incubated with C. neoformans, and nine other proteins were downregulated. The mRNA levels of S100A10 and peroxiredoxin I fluctuated with the protein levels. These results suggested that the expressions of peroxiredoxin I and S100A10 were regulated during the process of invasion of HUVECs by C. neoformans. We hypothesise that these proteins take part in the modifications of HUVEC cytoskeleton and the tolerance to oxidative stress, which may affect the process of invasion by C. neoformans.
authors.affiliation: Mycology Center and Department of Dermatology, Changzheng Hospital, Secondary Military Medical University, Shanghai, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Wang
authors.forename: Yuan-Jie
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Cui
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Julin
authors.initials: J
authors.lastname: Gu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Hai
authors.initials: H
authors.lastname: Wen
mesh_terms: D017306:Annexin A2 / Q000096:biosynthesis; D002478:Cells, Cultured; D018920:Coculture Techniques; D003455:Cryptococcus neoformans / Q000472:pathogenicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000737:chemistry* / Q000382:microbiology*; D020869:Gene Expression Profiling; D006801:Humans; D054464:Peroxiredoxins / Q000096:biosynthesis; D020543:Proteome / Q000032:analysis*; D009418:S100 Proteins / Q000096:biosynthesis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000099248:S100 Calcium Binding Protein A10
chemical_list: D017306:Annexin A2; D054464:Peroxiredoxins; D020543:Proteome; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10