title: ICSH recommendations for the measurement of haemoglobin F.
abstract: Measurement of the Haemoglobin F in red cell haemolysates is important in the diagnosis of δβ thalassaemia, hereditary persistence of fetal haemoglobin (HPFH) and in the diagnosis and management of sickle cell disease. The distribution of Hb F in red cells is useful in the diagnosis of HPFH and in the assessment of feto-maternal haemorrhage. The methods of quantifying Hb F are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high-performance liquid chromatography and capillary electrophoresis.
authors.affiliation: Department of Haematology, University College London Hospitals, London, UK. adrian.stephens@mac.com
authors.forename: A D
authors.initials: AD
authors.lastname: Stephens
authors.forename: M
authors.initials: M
authors.lastname: Angastiniotis
authors.forename: E
authors.initials: E
authors.lastname: Baysal
authors.forename: V
authors.initials: V
authors.lastname: Chan
authors.forename: B
authors.initials: B
authors.lastname: Davis
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
authors.forename: P C
authors.initials: PC
authors.lastname: Giordano
authors.forename: J D
authors.initials: JD
authors.lastname: Hoyer
authors.forename: A
authors.initials: A
authors.lastname: Mosca
authors.forename: B
authors.initials: B
authors.lastname: Wild
mesh_terms: D000468:Alkalies; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D005319:Fetal Hemoglobin / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D011489:Protein Denaturation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D013789:Thalassemia / Q000175:diagnosis*
chemical_list: D000468:Alkalies; D005319:Fetal Hemoglobin