title: Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model.
abstract: OBJECTIVE
  To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model.
  
  
  RESULTS
  Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg(-1) for measuring survival rate and 10 mg kg(-1) for cytokine measurement. The survival rate of mice treated with 200 mg kg(-1) specific IgY or 5 mg kg(-1) dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly (P < 0.05) decreased tumour necrosis factor-α (TNF-α) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-α than dexamethasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia.
  
  
  CONCLUSIONS
  The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-α and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver.
  
  
  CONCLUSIONS
  The specific IgY has potential for the treatment of LPS-induced endotoxemia.
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, China.
authors.forename: Y-H
authors.initials: YH
authors.lastname: Zhen
authors.forename: R
authors.initials: R
authors.lastname: Fang
authors.forename: C
authors.initials: C
authors.lastname: Ding
authors.forename: L-J
authors.initials: LJ
authors.lastname: Jin
authors.forename: X-Y
authors.initials: XY
authors.lastname: Li
authors.forename: Y-P
authors.initials: YP
authors.lastname: Diao
authors.forename: X-H
authors.initials: XH
authors.lastname: Shu
authors.forename: X-C
authors.initials: XC
authors.lastname: Ma
authors.forename: Y-P
authors.initials: YP
authors.lastname: Xu
mesh_terms: D000818:Animals; D002645:Chickens; D003907:Dexamethasone / Q000627:therapeutic use; D004530:Egg Yolk / Q000276:immunology; D019446:Endotoxemia / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D007136:Immunoglobulins / Q000627:therapeutic use*; D016753:Interleukin-10 / Q000097:blood; D008070:Lipopolysaccharides / Q000009:adverse effects*; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C016512:IgY; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D003907:Dexamethasone