title: An elastomeric patch electrospun from a blended solution of dermal extracellular matrix and biodegradable polyurethane for rat abdominal wall repair.
abstract: A biodegradable elastomeric scaffold was created by electrospinning a mixed solution of poly(ester urethane)urea (PEUU) and porcine dermal extracellular matrix (dECM) digest, with PEUU included to provide elasticity, flexibility, and mechanical support and dECM used to enhance bioactivity and biocompatibility. Micrographs and differential scanning calorimetry demonstrated partial miscibility between PEUU and dECM. With greater dECM content, scaffolds were found to possess lower breaking strains and suture retention strength, although initial modulus was greater with higher dECM concentrations. The hybrid scaffolds containing 0% to 50% dECM had tensile strengths of 5 to 7 MPa, breaking strains of 138% to 611%, initial moduli of 3 to 11 Mpa, and suture retention strengths of 35 to 59 MPa. When hydrated, scaffolds were found to contract markedly with 50% dECM content. When used in a rat full-thickness abdominal wall replacement model, no herniation, infection, or tissue adhesion was observed after 4 and 8 weeks with a scaffold containing 25% dECM or a control 100% PEUU scaffold. Scaffolds incorporating dECM were significantly thicker at the time of explant, with greater numbers of associated smooth muscle actin-positive staining cells than in the control, but minimal cellular infiltration and remodeling of the scaffold were detected regardless of dECM addition. The processing of dECM and PEUU from a mixed solution thus provided a scaffold with evidence of better bioactivity and with mechanical properties not achievable with digested dECM alone.
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Keisuke
authors.initials: K
authors.lastname: Takanari
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Amoroso
authors.forename: Ryotaro
authors.initials: R
authors.lastname: Hashizume
authors.forename: Ellen P
authors.initials: EP
authors.lastname: Brennan-Pierce
authors.forename: John M
authors.initials: JM
authors.lastname: Freund
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
mesh_terms: D034861:Abdominal Wall / Q000473:pathology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001673:Biodegradation, Environmental / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D020405:Dermis / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D020724:Elastomers / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D020007:Implants, Experimental; D008422:Materials Testing; D011140:Polyurethanes / Q000494:pharmacology*; D019919:Prosthesis Implantation; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions; D013314:Stress, Mechanical; D034421:Sus scrofa; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D020724:Elastomers; D011140:Polyurethanes; D012996:Solutions