title: Screening of donated whole globes for photorefractive keratectomy.
abstract: OBJECTIVE
  To introduce a screening method for diagnosis of photorefractive keratectomy (PRK) in donated whole globes.
  
  
  METHODS
  Donated whole globes with either no or an unknown history of refractive surgery on family interview were evaluated in the Central Eye Bank of Iran. After disinfection of globes by immersion in 3% povidone iodine for 3 minutes, followed by irrigation with 0.9% normal saline, gross inspection and slit-lamp biomicroscopic examination of the corneas were performed. Corneas with a disciform, round-shaped hazy area within the central 8 mm of the cornea, suggestive of previous PRK (case), and corneas without this criterion (control) were excised and sent for histopathology. Then, sensitivity and specificity of the screening method were calculated by analyzing the histopathology results of case and control corneas.
  
  
  RESULTS
  Twelve thousand five hundred fifty-four donated whole globes from 6832 donors were examined. Twenty case corneas from 10 donors and 54 controls from 27 donors were evaluated by histopathology. Age and sex of the donor population in both case and control groups were comparable with the entire donor population. PRK was confirmed by histopathology in all 20 case corneas but not in any of the control corneas. Both the sensitivity and specificity of this screening method were 100%.
  
  
  CONCLUSIONS
  Gross inspection of donated whole globes after exposure to povidone iodine followed by slit-lamp biomicroscopic examination, with particular attention to finding a midperipheral-to-midperipheral disciform hazy area within the central 8 mm of cornea, is a safe, simple, and inexpensive screening method, with high sensitivity and specificity for diagnosing PRK.
authors.affiliation: Ophthalmic Research Center, Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mrezaie47@yahoo.com
authors.forename: Mozhgan Rezaei
authors.initials: MR
authors.lastname: Kanavi
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Javadi
authors.forename: Tahereh
authors.initials: T
authors.lastname: Chamani
authors.forename: Atefeh
authors.initials: A
authors.lastname: Javadi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D003315:Cornea / Q000473:pathology* / Q000601:surgery; D003941:Diagnostic Techniques, Ophthalmological; D005125:Eye Banks; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009216:Myopia / Q000175:diagnosis* / Q000601:surgery; D018903:Photorefractive Keratectomy; D011206:Povidone-Iodine / Q000494:pharmacology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D011206:Povidone-Iodine