title: A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization.
abstract: The positioning of nucleosomes within the coding regions of eukaryotic genes is aligned with respect to transcriptional start sites. This organization is likely to influence many genetic processes, requiring access to the underlying DNA. Here, we show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset of locations distributed throughout coding regions where other factors are likely to be involved. These observations indicate that adenosine triphosphate-dependent remodeling enzymes are responsible for directing the positioning of the majority of nucleosomes within the Saccharomyces cerevisiae genome.
authors.affiliation: Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
authors.forename: Triantaffyllos
authors.initials: T
authors.lastname: Gkikopoulos
authors.forename: Pieta
authors.initials: P
authors.lastname: Schofield
authors.forename: Vijender
authors.initials: V
authors.lastname: Singh
authors.forename: Marina
authors.initials: M
authors.lastname: Pinskaya
authors.forename: Jane
authors.initials: J
authors.lastname: Mellor
authors.forename: Michaela
authors.initials: M
authors.lastname: Smolle
authors.forename: Jerry L
authors.initials: JL
authors.lastname: Workman
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Barton
authors.forename: Tom
authors.initials: T
authors.lastname: Owen-Hughes
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D042002:Chromatin Assembly and Disassembly; D004271:DNA, Fungal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D009154:Mutation; D009707:Nucleosomes / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D024363:Transcription Initiation Site
chemical_list: C489847:CHD1 protein, S cerevisiae; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C470546:ISW1 protein, S cerevisiae