title: [Establishment of a green fluorescent protein-labeled mouse model of adenoid cystic carcinoma].
abstract: OBJECTIVE
  To establish a novel nude mice model which can be visualized in real time and detected in a continuous and dynamic way for the development and metastasis of adenoid cystic carcinoma.
  
  
  METHODS
  Human adenoid cystic carcinoma cells, ACCM cell line, were infected with retroviral vector of pLEGFP-N1 and then screened for a single colony of ACCM-GFP cells. Cell proliferation and morphological analysis were conducted for ACCM and ACCM-GFP cells. Nude mice lingual carcinoma model was set up with ACCM-GFP cells injection and real time observation with fluorescence imaging on ACCM-GFP tumors was performed subsequently. Histological assay was analyzed for ACCM and ACCM-GFP tumors as well.
  
  
  RESULTS
  ACCM-GFP cells were able to express GFP stably in the long term. ACCM and ACCM-GFP cells showed no significant difference in cell proliferation and morphology, and no significant difference of histological characteristics in vivo could be found between ACCM and ACCM-GFP tumors. Tumor development could be monitored in real time with fluorescence imaging system in vivo.
  
  
  CONCLUSIONS
  GFP-expressing ACCM tumor model can be applied to detect and observe its development in the long term in a noninvasive, real time and dynamic way. It is also a kind of ideal in vivo mouse model for adenoid cystic carcinoma research.
authors.affiliation: Dept. of Oral and Maxillofacial Surgery, Qilu Hospital, Shandong University, Stomatology Center of Shandong University, Jinan 250012, China.
authors.forename: Zheng-gang
authors.initials: ZG
authors.lastname: Chen
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Dong
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Chengjun
authors.initials: C
authors.lastname: Zhou
authors.forename: Shanzhen
authors.initials: S
authors.lastname: Sun
authors.forename: Shaohua
authors.initials: S
authors.lastname: Liu
authors.forename: Fengcai
authors.initials: F
authors.lastname: Wei
mesh_terms: D000818:Animals; D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
chemical_list: D049452:Green Fluorescent Proteins