title: Method for somatic cell nuclear transfer in zebrafish.
abstract: Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish research community. In this chapter, we meticulously outline each step of the published protocol as well as preparations of equipments and reagents used in zebrafish SCNT. All describable detailed-tips are elaborated in texts and figures.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.
authors.forename: Kannika
authors.initials: K
authors.lastname: Siripattarapravat
authors.forename: Jose B
authors.initials: JB
authors.lastname: Cibelli
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D019976:Cloning, Organism / Q000379:methods*; D005260:Female; D008297:Male; D008845:Microinjections / Q000379:methods; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology; D015027:Zebrafish / Q000235:genetics*