title: [Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS].
abstract: OBJECTIVE
  To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS.
  
  
  METHODS
  The specimens were alkalized (pH > 10) and extracted with V (benzene):V(ethyl acetate) = 1:1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene):V(ethyl acetate) = 1:1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5.
  
  
  RESULTS
  The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%.
  
  
  CONCLUSIONS
  The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
authors.affiliation: Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
authors.forename: Shui-Qing
authors.initials: SQ
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Fei-Jun
authors.initials: FJ
authors.lastname: Gong
authors.forename: Zhong-Ping
authors.initials: ZP
authors.lastname: Wu
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Yu-Rong
authors.initials: YR
authors.lastname: Zhang
authors.forename: Run-Sheng
authors.initials: RS
authors.lastname: Zhang
mesh_terms: D000328:Adult; D014150:Antipsychotic Agents / Q000097:blood* / Q000506:poisoning; D002746:Chlorpromazine / Q000097:blood*; D003024:Clozapine / Q000097:blood*; D005260:Female; D053593:Forensic Toxicology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D014282:Trihexyphenidyl / Q000097:blood*
chemical_list: D014150:Antipsychotic Agents; D012997:Solvents; D014282:Trihexyphenidyl; D003024:Clozapine; D002746:Chlorpromazine