title: Pasteurella multocida involved in respiratory disease of wild chimpanzees.
abstract: Pasteurella multocida can cause a variety of diseases in various species of mammals and birds throughout the world but nothing is known about its importance for wild great apes. In this study we isolated P. multocida from wild living, habituated chimpanzees from Taï National Park, Côte d'Ivoire. Isolates originated from two chimpanzees that died during a respiratory disease outbreak in 2004 as well as from one individual that developed chronic air-sacculitis following this outbreak. Four isolates were subjected to a full phenotypic and molecular characterisation. Two different clones were identified using pulsed field gel electrophoresis. Multi Locus Sequence Typing (MLST) enabled the identification of previous unknown alleles and two new sequence types, ST68 and ST69, were assigned. Phylogenetic analysis of the superoxide dismutase (sodA) gene and concatenated sequences from seven MLST-housekeeping genes showed close clustering within known P. multocida isolated from various hosts and geographic locations. Due to the clinical relevance of the strains described here, these results make an important contribution to our knowledge of pathogens involved in lethal disease outbreaks among endangered great apes.
authors.affiliation: Research Group Emerging Zoonoses, Robert Koch-Institut, Berlin, Germany. KoendgenS@rki.de
authors.forename: Sophie
authors.initials: S
authors.lastname: Köndgen
authors.forename: Michaela
authors.initials: M
authors.lastname: Leider
authors.forename: Felix
authors.initials: F
authors.lastname: Lankester
authors.forename: Astrid
authors.initials: A
authors.lastname: Bethe
authors.forename: Antina
authors.initials: A
authors.lastname: Lübke-Becker
authors.forename: Fabian H
authors.initials: FH
authors.lastname: Leendertz
authors.forename: Christa
authors.initials: C
authors.lastname: Ewers
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics; D007560:Cote d'Ivoire / Q000453:epidemiology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004196:Disease Outbreaks / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000453:epidemiology / Q000382:microbiology; D002679:Pan troglodytes / Q000382:microbiology*; D010326:Pasteurella Infections / Q000453:epidemiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D012140:Respiratory Tract Diseases / Q000453:epidemiology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000145:classification / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase