title: [Time-dependent recruitment and differentiation of fibrocytes in mouse skin wound healing].
abstract: OBJECTIVE
  To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing.
  
  
  METHODS
  Fibrocytes (expressing CD45 and procollagen I ) and myofibroblasts (expressing CD45 and alpha-SMA) were co-localized by immunofluorescent staining. The number of fibrocytes and myofibroblasts was counted at different post-wounding interval.
  
  
  RESULTS
  At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding.
  
  
  CONCLUSIONS
  During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibrocytes may provide new information for wound age determination.
authors.affiliation: School of Forensic Medicine, China Medical University, Shenyang 110001, China. yyfan998@yahoo.com
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Fan
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Guan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Liu
authors.forename: Ji-Long
authors.initials: JL
authors.lastname: Zheng
authors.forename: Zhen-Bin
authors.initials: ZB
authors.lastname: Zhao
authors.forename: Tian-Shui
authors.initials: TS
authors.lastname: Yu
authors.forename: Wen-Xiang
authors.initials: WX
authors.lastname: Ma
mesh_terms: D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D058628:Myofibroblasts / Q000166:cytology / Q000378:metabolism; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling; D013997:Time Factors; D014945:Wound Healing
chemical_list: D017493:Leukocyte Common Antigens