title: miRNA-1 targets fibronectin1 and suppresses the migration and invasion of the HEp2 laryngeal squamous carcinoma cell line.
abstract: MicroRNAs (miRNAs) are an evolutionarily conserved class of endogenous, non-coding RNAs that modulate gene expression at the post-transcriptional level and are involved in tumorigenesis. In this study, we demonstrate that miR-1 suppresses the potential for growth, migration and invasion in the HEp2 cell line. Furthermore, we validate that FN1 is a direct target gene for miR-1 via fluorescent reporter assay and is negatively regulated by miR-1. Moreover, the knockdown of FN1 has the same phenotypic effects as the overexpression of miR-1. Taken together, our results provide evidence that miR-1 may play a role as a tumor suppressor gene in laryngeal carcinoma.
authors.affiliation: Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.forename: Ge
authors.initials: G
authors.lastname: Song
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Tang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C067625:FN1 protein, human; D005353:Fibronectins; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm