title: Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe.
abstract: A clickable, affinity-based probe (AfBP), which was modified from staurosporine (a natural product kinase inhibitor), has been synthesized and used in situ for activity-based proteome profiling of potential cellular targets of staurosporine in HepG2 cancer cells.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.
authors.forename: Haibin
authors.initials: H
authors.lastname: Shi
authors.forename: Xiamin
authors.initials: X
authors.lastname: Cheng
authors.forename: Siu Kwan
authors.initials: SK
authors.lastname: Sze
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D057930:Click Chemistry; D056945:Hep G2 Cells; D006801:Humans; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D010539:Permeability; D047428:Protein Kinase Inhibitors / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D019311:Staurosporine / Q000378:metabolism*
chemical_list: D015335:Molecular Probes; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D019311:Staurosporine