title: PEGylated poly(ethylene imine) copolymer-delivered siRNA inhibits HIV replication in vitro.
abstract: RNA interference is increasingly being utilized for the specific targeting and down-regulation of disease-causing genes, including targeting viral infections such as HIV. T lymphocytes, the primary target for HIV, are very difficult to treat with gene therapy applications such as RNA interference because of issues with drug delivery. To circumvent these problems, we investigated poly(ethylene imine) (PEI) as a method of improving transfection efficiency of siRNA to T lymphocytes. Additionally, polyethylene glycol (PEG) moieties were engrafted to the PEI polymers with the goals of improving stability and reducing cytotoxicity. Initial studies on PEG-PEI/siRNA polyplex formation, size and their interaction with cell membranes demonstrated their feasibility as drug delivery agents. Assays with lymphocytes revealed low cytotoxicity profiles of the polyplexes at pharmacologically relevant concentrations with PEGylated copolymers obtaining the best results. Successful transfection of a T cell line or primary T cells with siRNA was observed via flow cytometry and confocal microscopy. Finally, the biological effect of copolymer-delivered siRNA was measured. Of particular significance, siRNA targeted to the HIV gene nef and delivered by one of the PEG-PEI copolymers in repetitive treatments every 2-3 days was observed to inhibit HIV replication to the same extent as azidothymidine over the course of 15 days.
authors.affiliation: Laboratorio Inmunobiología Molecular, Hospital General Universitario Gregorio Marañón, C/Doctor Esquerdo 46, 28007 Madrid, Spain.
authors.forename: Nick D
authors.initials: ND
authors.lastname: Weber
authors.forename: Olivia M
authors.initials: OM
authors.lastname: Merkel
authors.forename: Thomas
authors.initials: T
authors.lastname: Kissel
authors.forename: María Ángeles
authors.initials: MÁ
authors.lastname: Muñoz-Fernández
mesh_terms: D019380:Anti-HIV Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D006678:HIV / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D014779:Virus Replication / Q000187:drug effects* / Q000502:physiology
chemical_list: D019380:Anti-HIV Agents; D034741:RNA, Small Interfering; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); D011092:Polyethylene Glycols; D011094:Polyethyleneimine