title: Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
abstract: We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
authors.affiliation: Department of Urology, The David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA.
authors.forename: Mayank
authors.initials: M
authors.lastname: Patel
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Colin
authors.initials: C
authors.lastname: Halford
authors.forename: Michael A
authors.initials: MA
authors.lastname: Lewinski
authors.forename: Elliot M
authors.initials: EM
authors.lastname: Landaw
authors.forename: Bernard M
authors.initials: BM
authors.lastname: Churchill
authors.forename: David A
authors.initials: DA
authors.lastname: Haake
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology
chemical_list: D015345:Oligonucleotide Probes; D012336:RNA, Ribosomal, 16S