title: Modulation of FAD-dependent monooxygenase activity from aromatic compounds-degrading Stenotrophomonas maltophilia strain KB2.
abstract: The purpose of this study was purification and characterization of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2, enzyme that catabolises phenol and its derivatives through the initial hydroxylation to catechols. The enzyme  requires NADH and FAD as a cofactors for activity, catalyses hydroxylation of a wide range of monocyclic phenols, aromatic acids and dihydroxylated derivatives of benzene except for catechol. High activity of this monooxygenase was observed in cell extract of strain KB2 grown on phenol, 2-methylphenol, 3-metylphenol or 4-methylphenol. Ionic surfactants as well as cytochrome P450 inhibitors or 1,4-dioxane, acetone and n-butyl acetate inhibited the enzyme activity, while non-ionic surfactants, chloroethane, ethylbenzene, ethyl acetate, cyclohexane, and benzene enhanced it. These results indicate that the phenol monooxygenase from Stenotrophomonas maltophilia strain KB2 holds great potential for bioremediation.
authors.affiliation: Department of Biochemistry, University of Silesia in Katowice, Katowice, Poland.
authors.forename: Danuta
authors.initials: D
authors.lastname: Wojcieszyńska
authors.forename: Izabela
authors.initials: I
authors.lastname: Greń
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hupert-Kocurek
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
mesh_terms: D001554:Benzene / Q000378:metabolism; D001673:Biodegradation, Environmental; D002396:Catechols / Q000378:metabolism; D003408:Cresols / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D004146:Dioxanes / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D019800:Phenol / Q000378:metabolism; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D002396:Catechols; D003408:Cresols; D065607:Cytochrome P-450 Enzyme Inhibitors; D004146:Dioxanes; D004791:Enzyme Inhibitors; D013501:Surface-Active Agents; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; C032538:4-cresol; D019800:Phenol; D006899:Mixed Function Oxygenases; C042041:3-cresol; D001554:Benzene; C025223:1,4-dioxane; C034221:catechol; C034047:2-cresol