title: Biocatalytic production of (2S,3S)-2,3-butanediol from diacetyl using whole cells of engineered Escherichia coli.
abstract: (2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a crucial chiral compound that acts as an excellent building block in asymmetric synthesis of highly valuable chiral compounds. However, the low concentration and optical purity of (2S,3S)-2,3-BD produced in previous studies limited its applications. In the present work, the gene encoding 2,3-butanediol dehydrogenase from an Enterobacter cloacae ssp. dissolvens strain SDM was cloned and expressed in Escherichia coli. Whole cells of the recombinant E. coli was used to produce (2S,3S)-2,3-BD from diacetyl. Under optimal conditions, high-optical-purity (2S,3S)-2,3-BD (purity >99%) was obtained with concentrations of 16.1 g l(-1) and 26.8 g l(-1) in batch and fed-batch conversions, respectively. Thus, the process might be a promising alternative for the production of (2S,3S)-2,3-BD.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Ailong
authors.initials: A
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Tao
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D061249:Batch Cell Culture Techniques; D055162:Biocatalysis; D053898:Biosynthetic Pathways; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D003001:Cloning, Molecular; D003931:Diacetyl / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005240:Feasibility Studies; D005818:Genetic Engineering / Q000379:methods*; D011995:Recombination, Genetic / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; D003931:Diacetyl