title: Urinary protein profiling by liquid chromatography/tandem mass spectrometry: ADAM28 is overexpressed in bladder transitional cell carcinoma.
abstract: Bladder cancer is the most common urological cancer with higher incidence rate in the endemic areas of Blackfoot disease (BFD) in southern Taiwan. The aim of this study was to utilize the proteomic approach to establish urinary protein patterns of bladder cancer. The experimental results showed that most patients with bladder cancer had proteinuria or albuminuria. The urine arsenic concentrations of bladder cancer patients in BFD areas were significantly higher than those patients from non-BFD areas. In the proteomic analysis, the urinary proteome was identified by nano-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (nano-HPLC/ESI-MS/MS) followed by peptide fragmentation pattern analysis. We categorized 2782 unique proteins of which 89 proteins were identified with at least three unique matching peptide sequences. Among these 89 proteins, thirteen of them were not found in the control group and may represent proteins specific for bladder cancer. In this study, three proteins, SPINK5, ADAM28 and PTP1, were also confirmed by Western blotting and showed significant differential expression compared with the control group. ADAM28 may be used as a possible biomarker of bladder cancer.
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Taiwan. yctyan@kmu.edu.tw
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.forename: Sharon Chia-Ju
authors.initials: SC
authors.lastname: Chen
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Yang
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Pao-Chi
authors.initials: PC
authors.lastname: Liao
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis / Q000652:urine*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D001151:Arsenic / Q000652:urine; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000652:urine*; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000652:urine*; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000652:urine; D016491:Peripheral Vascular Diseases; D053491:Proteinase Inhibitory Proteins, Secretory / Q000652:urine; D011506:Proteins / Q000032:analysis / Q000145:classification; D011507:Proteinuria / Q000652:urine; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000074163:Serine Peptidase Inhibitor Kazal-Type 5; D013624:Taiwan; D053719:Tandem Mass Spectrometry / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000652:urine*
chemical_list: D014408:Biomarkers, Tumor; D010446:Peptide Fragments; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D020543:Proteome; C120650:SPINK5 protein, human; D000074163:Serine Peptidase Inhibitor Kazal-Type 5; D051722:ADAM Proteins; C410224:ADAM28 protein, human; D001151:Arsenic