title: Sequencing of CJIE1 prophages from  Campylobacter jejuni  isolates reveals the presence of inserted and (or) deleted genes.
abstract: Bacteriophages capable of integrating into host bacterial genomes as prophages affect the biology and virulence of their bacterial hosts. Previously, partial sequencing of 12 prophages similar to CJIE1 from Campylobacter jejuni RM1221 did not show the presence of inserted nonphage genes. Therefore, four of these prophages were sequenced completely, and indels were found in at least two different regions of the prophage genome. Putative proteins from one indel appeared to be members of two new families of proteins, with proteins within each family related to each other by a common domain. Further heterogeneity was found adjacent to the CJE0270 homolog, creating difficulty locating the end of the prophage on this side and in determining the composition of the core prophage. These prophages appear to comprise a family that has heterogeneity in gene content resulting from insertion or deletion of additional genes at three locations in their genomes. In addition, members of the CJIE1 phage family may differ somewhat in their biology from phage Mu. Further investigations of these Campylobacter prophages can be expected to provide interesting insights into the biology of the phages themselves and into the role of these phages in the biology of their hosts.
authors.affiliation: Enterics Research Section, National Microbiology Laboratory, Public Health Agency of Canada, Canadian Science Centre for Human and Animal Health, Winnipeg, Canada. Clifford.Clark@phac-aspc.gc.ca
authors.forename: Clifford G
authors.initials: CG
authors.lastname: Clark
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D016123:Campylobacter jejuni / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005814:Genes, Viral; D016679:Genome, Viral; D054643:INDEL Mutation; D039002:Prophages / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral