title: Applications of 'TissueQuant'- a color intensity quantification tool for medical research.
abstract: This paper demonstrates the use of TissueQuant - an image analysis tool for quantification of color intensities which was developed for use in medical research where the stained biological specimen such as tissue or antigen needs to be quantified. TissueQuant provides facilities for user interaction to choose and quantify the color of interest and its shades. Gaussian weighting functions are used to provide a color score which quantifies how close the shade is to the user specified reference color. We describe two studies in medical research which use TissueQuant for quantification. The first study evaluated the effect of petroleum-ether extract of Cissus quadrangularis (CQ) on osteoporotic rats. It was found that the analysis results correlated well with the manual evaluation, p < 0.001. The second study evaluated the nerve morphometry and it was found that the adipose and non adipose tissue content was maximum in radial nerve among the five nerves studied.
authors.affiliation: Manipal Centre for Information Science, Manipal University, India. keerthana.prasad@manipal.edu
authors.forename: Keerthana
authors.initials: K
authors.lastname: Prasad
authors.forename: Bhagath Kumar
authors.initials: BK
authors.lastname: P
authors.forename: Marx
authors.initials: M
authors.lastname: Chakravarthy
authors.forename: Gopalakrishna
authors.initials: G
authors.lastname: Prabhu
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000465:Algorithms; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D032422:Cissus; D003116:Color; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000706:statistics & numerical data*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010024:Osteoporosis / Q000188:drug therapy / Q000201:enzymology / Q000473:pathology; D010525:Peripheral Nerves / Q000033:anatomy & histology; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D012985:Software Design; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D010936:Plant Extracts; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase