title: Nongenomic glucocorticoid receptor action regulates gap junction intercellular communication and neural progenitor cell proliferation.
abstract: Glucocorticoids (GCs) are used to treat pregnant women at risk for preterm delivery; however, prenatal exposure to GCs may trigger adverse neurological side effects due to reduced neural progenitor cell (NPC) proliferation. Whereas many established cell-cycle regulators impact NPC proliferation, other signaling molecules, such as the gap junction protein connexin-43 (Cx43), also influence proliferation. Gap junction intercellular communication (GJIC) is influenced by GCs in some cells, but such hormone effects have not been examined in coupled stem cells. We found that both continuous and transient exposure of embryonic day 14.5 mouse neurosphere cultures to dexamethasone (DEX) limits proliferation of coupled NPCs, which is manifested by both a reduction in S-phase progression and enhanced cell-cycle exit. A short (i.e., 1-h) DEX treatment also reduced GJIC as measured by live-cell fluorescence recovery after photobleaching, and altered the synchrony of spontaneous calcium transients in coupled NPCs. GC effects on GJIC in NPCs are transcription-independent and mediated through plasma membrane glucocorticoid receptors (GRs). This nongenomic pathway operates through lipid raft-associated GRs via a site-specific, MAPK-dependent phosphorylation of Cx43, which is linked to GR via caveolin-1 (Cav-1) and c-src. Cav-1 is essential for this nongenomic action of GR, as DEX effects on GJIC, Cx43 phosphorylation, and MAPK activation are not observed in Cav-1 knockout NPCs. As transient pharmacologic inhibition of GJIC triggers reduced S-phase progression but not enhanced cell-cycle exit, the nongenomic GR signaling pathway may operate via distinct downstream effectors to alter the proliferative capacity of NPCs.
authors.affiliation: Department of Neuroscience, Pittsburgh Institute for Neurodegenerative Disease, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA.
authors.forename: Ranmal Aloka
authors.initials: RA
authors.lastname: Samarasinghe
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Maio
authors.forename: Daniela
authors.initials: D
authors.lastname: Volonte
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Galbiati
authors.forename: Marcia
authors.initials: M
authors.lastname: Lewis
authors.forename: Guillermo
authors.initials: G
authors.lastname: Romero
authors.forename: Donald B
authors.initials: DB
authors.lastname: DeFranco
mesh_terms: D000818:Animals; D015153:Blotting, Western; D051242:Caveolin 1 / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018031:Connexin 43 / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D036681:Fluorescence Recovery After Photobleaching; D017629:Gap Junctions / Q000502:physiology*; D005938:Glucocorticoids / Q000494:pharmacology*; D051379:Mice; D058953:Neural Stem Cells / Q000502:physiology*; D010766:Phosphorylation; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D018031:Connexin 43; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone