title: A physicochemical-biotechnological approach for an integrated valorization of olive mill wastewater.
abstract: An integrated physicochemical-biotechnological approach for a multipurpose valorization of olive mill wastewaters was studied. More than 60% of the wastewater natural polyphenols were recovered through a solid phase extraction procedure, by employing Amberlite XAD16 resin as the adsorbent and ethanol as the biocompatible desorbing phase. Thereafter, the dephenolized effluent was fed to a mesophilic anaerobic acidogenic packed-bed biofilm reactor for the bioconversion of the organic leftover into volatile fatty acids (VFAs). A VFAs concentration of 19 gCODL(-1) was obtained, representing more than 70% of the COD occurring in the anaerobic effluent. The biotechnological process was assessed by means of bio-molecular analyses, which showed that the reactor packed bed was mostly colonized by bacteria of the Firmicutes phylogenetic group. The biorefinery scheme developed in this study allowed the obtainment of 1.59 g of polyphenols per liter of wastewater treated and 2.72 gCODL(-1) day(-1) of VFAs.
authors.affiliation: Department of Civil, Environmental and Materials Engineering (DICAM), Unit of Environmental Biotechnology and Biorefineries, Faculty of Engineering, University of Bologna, via Terracini 28, 40131 Bologna, Italy. alberto.scoma2@unibo.it
authors.forename: Alberto
authors.initials: A
authors.lastname: Scoma
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Bertin
authors.forename: Giulio
authors.initials: G
authors.lastname: Zanaroli
authors.forename: Serena
authors.initials: S
authors.lastname: Fraraccio
authors.forename: Fabio
authors.initials: F
authors.lastname: Fava
mesh_terms: D001419:Bacteria / Q000235:genetics; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D055598:Chemical Phenomena; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000032:analysis; D005740:Gases / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000302:isolation & purification; D010802:Phylogeny; D052616:Solid Phase Extraction; D013696:Temperature; D013997:Time Factors; D014865:Waste Disposal, Fluid / Q000379:methods
chemical_list: D005232:Fatty Acids, Volatile; D005740:Gases; D007220:Industrial Waste; D010636:Phenols