title: Composition of hydroponic lettuce: effect of time of day, plant size, and season.
abstract: BACKGROUND
  The diurnal variation of nitrate and sugars in leafy green vegetables may vary with plant size or the ability of plants to buffer the uptake, synthesis, and use of metabolites. Bibb lettuce was grown in hydroponics in a greenhouse and sampled at 3 h intervals throughout one day in August 2007 and another day in November 2008 to determine fresh weight, dry matter, and concentration of nitrate and sugars. Plantings differing in size and age were sampled on each date.
  
  
  RESULTS
  The dry/fresh weight ratio increased during the daylight period. This increase was greater for small compared to large plants. On a fresh weight basis, tissue nitrate of small plants was only half that of larger plants. The variation in concentration with time was much less for nitrate than for soluble sugars. Soluble sugars were similar for all plant sizes early in the day, but they increased far more for small compared to large plants in the long days of summer.
  
  
  CONCLUSIONS
  The greatest yield on a fresh weight basis was obtained by harvesting lettuce at dawn. Although dry matter or sugar content increased later in the day, there is no commercial benefit to delaying harvest as consumers do not buy lettuce for these attributes.
authors.affiliation: Department of Forestry and Horticulture, Connecticut Agricultural Experiment Station, New Haven, CT 06504, USA. Martin.Gent@ct.gov.
authors.forename: Martin P N
authors.initials: MP
authors.lastname: Gent
mesh_terms: D002940:Circadian Rhythm; D003237:Connecticut; D018556:Crops, Agricultural / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D004040:Dietary Carbohydrates / Q000032:analysis* / Q000378:metabolism; D006601:Hexoses / Q000032:analysis / Q000096:biosynthesis; D018527:Hydroponics / Q000379:methods; D018545:Lactuca / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D009566:Nitrates / Q000032:analysis* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D012621:Seasons; D012995:Solubility; D013472:Sunlight
chemical_list: D004040:Dietary Carbohydrates; D006601:Hexoses; D009566:Nitrates