title: Multivariate optical computing using a digital micromirror device for fluorescence and Raman spectroscopy.
abstract: A multivariate optical computer has been constructed consisting of a spectrograph, digital micromirror device, and photomultiplier tube that is capable of determining absolute concentrations of individual components of a multivariate spectral model. We present experimental results on ternary mixtures, showing accurate quantification of chemical concentrations based on integrated intensities of fluorescence and Raman spectra measured with a single point detector. We additionally show in simulation that point measurements based on principal component spectra retain the ability to classify cancerous from noncancerous T cells.
authors.affiliation: Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, California 95817, USA.
authors.forename: Zachary J
authors.initials: ZJ
authors.lastname: Smith
authors.forename: Sven
authors.initials: S
authors.lastname: Strombom
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wachsmann-Hogiu
mesh_terms: D002469:Cell Separation; D003936:Diagnosis, Computer-Assisted; D004867:Equipment Design; D005456:Fluorescent Dyes; D006801:Humans; D019169:Jurkat Cells; D015458:Leukemia, T-Cell / Q000473:pathology; D015999:Multivariate Analysis; D055620:Optical Phenomena; D025341:Principal Component Analysis; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D005456:Fluorescent Dyes