title: Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
abstract: The immunomodulatory activity of Cassia auriculata (CA)-derived polyphenols was tested on aged rats. Rats (24-26 months old) were given CA polyphenols supplementation at doses of 25, 50, and 100 mg/kg for 28 days. Flow cytometry analysis of CA polyphenols-treated aged rats showed increased T and B cells percentage along with enhanced proliferation of splenocytes in both resting and LPS-stimulated cells. Increased percentage of pan T cells is further supported by an elevation of CD4+, CD8+, and CD4+CD25+ regulatory cells. In terms of innate immune cell activity, CA polyphenol supplementation reduced the oxidative burst activity of neutrophils in response to PMA and Escherichia coli activation. Our results collectively show that polyphenols derived from CA boost T cell immunity by increasing the number of T cells and its sensitivity towards stimulants and decreasing ROS production by neutrophils that could potentially harm multiple biological systems in aged individuals.
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia.
authors.forename: Cini M
authors.initials: CM
authors.lastname: John
authors.forename: Pratheep
authors.initials: P
authors.lastname: Sandrasaigaran
authors.forename: Chih Kong
authors.initials: CK
authors.lastname: Tong
authors.forename: Aishah
authors.initials: A
authors.lastname: Adam
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ramasamy
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D002366:Cassia / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D035264:Flowers / Q000276:immunology; D007155:Immunologic Factors / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D059808:Polyphenols / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007155:Immunologic Factors; D008070:Lipopolysaccharides; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013755:Tetradecanoylphorbol Acetate