title: Biochemical activities of Brochothrix thermosphacta.
abstract: Activities of 19 hydrolases were estimated in cell suspensions of 40 Brochothrix thermosphacta strains, isolated from meat and meat products packaged under various conditions, by using API ZYM® test. These strains produced: acidic phosphatase, esterase C4, esterase/lipase C8, α-chymotrypsin, leucine arylamidase, β-glucosidase and α-glucosidase. The predominating biotype produced only the first four of the listed enzymes. The extracellular lipolytic activity for bromo-chloro-indolyl-caprylate was detected only in culture broth supernatants of 4 strains thereby indicating that esterase/lipase C8 is cell-bound. 13 of the strains displayed proteolytic activity for albumin at 4 °C (0.31-2.07 U) while 9 strains showed this activity at 25 °C (0.11-1.21 U). Only 4 strains digested albumin at both temperatures. Thus the meat spoilage potential of B. thermosphacta strains results not only from digestion of carbohydrates but also from their proteolytic activity.
authors.affiliation: Technical University of Lodz, Institute of Fermentation Technology and Microbiology Wolczanska 171/173, 90-924 Lodz, Poland. agnieszka.nowak@p.lodz.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Nowak
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Piotrowska
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000626:Aminopeptidases; D058640:Brochothrix / Q000201:enzymology* / Q000302:isolation & purification*; D002918:Chymotrypsin / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D005506:Food Contamination; D005516:Food Microbiology; D006867:Hydrolases / Q000378:metabolism*; D008049:Lipase / Q000378:metabolism; D008461:Meat Products / Q000382:microbiology*; D013696:Temperature; D000520:alpha-Glucosidases; D001617:beta-Glucosidase
chemical_list: D006867:Hydrolases; D004950:Esterases; D008049:Lipase; D000135:Acid Phosphatase; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D000626:Aminopeptidases; D002918:Chymotrypsin; C117608:alpha-chymotrypsin