title: Mineral to matrix ratio determines biomaterial and biomechanical properties of rat femur--application of Fourier transform infrared spectroscopy.
abstract: We studied the changes of biomaterial and biomechanical properties of the rat femur during development. Thirty male Wistar rats were allocated to 6 groups: aged 6 weeks (n=5), 9 weeks (n=5), 12 weeks (n=5), 15 weeks (n=5), 24 weeks (n=5), and 36 weeks (n=5). The mineral to matrix ratio (M/M ratio) of rat femur by Fourier transform infrared spectroscopy was 0.97 ± 0.10 at the age of 6 weeks, and reached the maximum of 1.52 ± 0.17 at the age of 36 weeks. Total bone mineral density (BMD) by peripheral quantitative computed tomography of the femoral shaft aged 6 weeks was 479.1 ± 58.7 mg/cm(3), and reached the maximum of 1022.2 ± 42.3 mg/cm(3) at the age of 36 weeks. The ultimate load to failure of the femur of the rat aged 6 weeks by the three-point bending test was 29.6 ± 6.1 N. At the age of 36 weeks, the ultimate load to failure of the rat femur increased to the maximum of 283.5 ± 14.7 N. The results showed that the M/M ratio increased with development as total BMD and bone strength increased. The results suggest that the M/M ratio is one of the determinants of the biomaterial and biomechanical properties of bone.
authors.affiliation: Division of Rehabilitation, Tokushima University Hospital, Tokushima, Japan.
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Takata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yonezu
authors.forename: Akira
authors.initials: A
authors.lastname: Shibata
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Enishi
authors.forename: Nori
authors.initials: N
authors.lastname: Sato
authors.forename: Mitsuhiko
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shigetaka
authors.initials: S
authors.lastname: Nakao
authors.forename: Koji
authors.initials: K
authors.lastname: Komatsu
authors.forename: Natsuo
authors.initials: N
authors.lastname: Yasui
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001696:Biomechanical Phenomena; D001835:Body Weight; D015519:Bone Density; D001857:Bone Matrix / Q000737:chemistry*; D005269:Femur / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D001672:Biocompatible Materials