title: [Effect of the costimulatory molecules OX40-OX40 ligand interaction on the expression of NFATc1 in leukocytes of apolipoprotein E-deficient mice].
abstract: OBJECTIVE
  To investigate the effect of OX40/OX40L interaction on the nuclear factor of activated T cells c1 (NFATc1) in ApoE-/- mice.
  
  
  METHODS
  Lymphocytes were prepared from mouse spleens after Collar-treated Surgery, then incubated with a range of agonistic anti-OX40 mAbs and inhibitory anti-OX40L mAb to stimulate or inhibit OX40-OX40L interaction in vitro. The expression of NFATc1 mRNA and protein in lymphocytes of ApoE-/- mice was measured by Real Time PCR and flow cytometry, respectively.
  
  
  RESULTS
  (1) After stimulating OX40-OX40L signal pathway, the expression of NFATc1 mRNA and protein in leukocytes of ApoE-/- mice was significantly increased, with maximal effect occurring at 20 µg/ml anti-OX40 mAb-stimulated, and peaked at 24 h at any concentration (P < 0.01). (2) Anti-OX40L mAb significantly suppressed the expression of NFATc1 in leukocytes of ApoE-/- mice, with maximal effect occurring at 20 µg/ml anti-OX40L mAb, and peaked at 24 h (P < 0.001).
  
  
  CONCLUSIONS
  OX40-OX40L interaction can regulate the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE-/- mice.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Liang-Jie
authors.initials: LJ
authors.lastname: Xu
authors.forename: Jin-Chuan
authors.initials: JC
authors.lastname: Yan
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.forename: Pei-Jing
authors.initials: PJ
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Gong
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Wang
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D050778:NFATC Transcription Factors / Q000378:metabolism*; D053262:Receptors, OX40 / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D001057:Apolipoproteins E; D050778:NFATC Transcription Factors; C494005:Nfatc1 protein, mouse; D053262:Receptors, OX40; C505717:Tnfrsf4 protein, mouse