title: Simultaneous estimation of hydroxychavicol and chlorogenic acid from Piper betel L. through RP-HPLC.
abstract: A RP-HPLC method was developed (λ (max) =280) to quantify hydroxychavicol and chlorogenic acid in Piper betel Linn. The method was validated for linearity, limit of detection (LOD=3:1σ/S), limit of quantification (LOQ=10:1σ/S), precision, accuracy and ruggedness. The response was linear with good correlation between concentration and mean peak area through a coefficient of determinants (r (2)) of 0.9940, y=1.98e +004x +5.19e +004 and 0.9945, y=2.76e+004x+1.40e+005 with LOD 1.6 µg mL(-1), 1.0 µg mL(-1) and LOQ 5.0 µg mL(-1) and 3.0 µg mL(-1), respectively, for hydroxychavicol (28.56% w/w) and chlorogenic acid (0.40% w/w). The %RSD of precision and recovery of hydroxychavicol and chlorogenic acid were <2.0%. The proposed method was simple, accurate, specific, precise and reproducible.
authors.affiliation: Department of Pharmaceutical Technology, School of Natural Product Studies, Jadavpur University, Kolkata 700 032, India.
authors.forename: Niladri
authors.initials: N
authors.lastname: Maity
authors.forename: Neelesh K
authors.initials: NK
authors.lastname: Nema
authors.forename: Mythies K
authors.initials: MK
authors.lastname: Sellamuthu
authors.forename: Birendra K
authors.initials: BK
authors.lastname: Sarkar
authors.forename: Pulok K
authors.initials: PK
authors.lastname: Mukherjee
mesh_terms: D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005054:Eugenol / Q000031:analogs & derivatives* / Q000032:analysis; D031701:Piper / Q000737:chemistry*
chemical_list: C051268:2-hydroxychavicol; D002726:Chlorogenic Acid; D005054:Eugenol