title: A comparison of neurotoxicity in cerebellum produced by dermal application of chlorpyrifos in young and adult mice.
abstract: Chlorpyrifos (CPF), an organophosphate pesticide inhibits acetylcholinesterase (AChE) and causes neuromuscular incoordination among children and elderly. The objectives of the present study were to compare the neurotoxic effects of dermal application of CPF on the cerebellum in the parameters of glial fibrillary acidic protein (GFAP) expression in young and adult mice and to correlate with the changes in acetylcholinesterase levels. Male Balb/c mice, 150 days old (adult) and 18 days old (young) were dermally applied with ½ LD(50) of CPF over the tails for 14 days. Serum AChE concentration was estimated and GFAP immunostaining was performed on sagittal paraffin sections through the vermis of cerebellum. Although reduced in both age-groups exposed to CPF, percentage of reduction in serum AChE was more in adult compared to the young. Under GFAP immunostaining, brown colour fibres and glial cells were observed in cerebellar cortex and medulla in both the experimental groups. The mean GFAP-positive glial cell count in cerebellar medulla per mm(2) of section was significantly (p < 0.05) increased in adult mice exposed to CPF when compared with age-matched control. In conclusion, this study confirmed that dermal exposure of CPF was able to exert neurotoxic effect in both young and adult mice. However, the quantitative results revealed that adult mice showed more GFAP expression in cerebellum when compared with the young, when exposed to CPF.
authors.affiliation: School of Pharmacy and Health Science, International Medical University, No. 126, Jalan 19/155B, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
authors.forename: K
authors.initials: K
authors.lastname: Krishnan
authors.forename: N K
authors.initials: NK
authors.lastname: Mitra
authors.forename: L S
authors.initials: LS
authors.lastname: Yee
authors.forename: H M
authors.initials: HM
authors.lastname: Yang
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000279:Administration, Cutaneous; D000367:Age Factors; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D004390:Chlorpyrifos / Q000008:administration & dosage / Q000633:toxicity*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000633:toxicity*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D002800:Cholinesterase Inhibitors; D005904:Glial Fibrillary Acidic Protein; D007306:Insecticides; D000110:Acetylcholinesterase; D004390:Chlorpyrifos