title: High-performance liquid chromatography/tandem mass spectrometry method for the determination of arbidol in human plasma.
abstract: An HPLC/MS/MS method for the determination of arbidol in human plasma was developed. Arbidol and internal standard (loratadine) were extracted from alkaline plasma with tert-butyl methyl ether and analyzed on a Zorbax SB C18 column (30 x 2.1 mm id, 3.5 microm particle size). The detection was by monitoring arbidol at m/z 479.1 --> 434.1 and the internal standard at m/z 383.2 --> 337.2. The method was validated according to U.S. Food and Drug Administration guidelines. The calibration curve was linear over the range of 0.5-500 ng/mL using a 100 microL sample volume. The intraday and interday precisions were less than 6.5%, and acceptable values were obtained for accuracy, recovery, and sensitivity. The developed method was selective, simple, sensitive, and easily applicable.
authors.affiliation: Peking University Third Hospital, Department of Pharmacy, Beijing, 100191, People's Republic of China, and Peking University, Therapeutic Drug Monitoring and Clinical Toxicology Center, Beijing, 100191, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xiong
authors.forename: Suodi
authors.initials: S
authors.lastname: Zhai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000097:blood*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013440:Sulfides
chemical_list: D007211:Indoles; C086979:umifenovir; D013440:Sulfides