title: 1H, 13C, and 15N resonance assignments for human regenerating family Iα protein.
abstract: Human regenerating (Reg) genes belong to the C-type lectin superfamily and express secretory proteins in various tissues. Reg Iα, also named lithostathine, has multiple roles in numerous biological events such as cytokines, anti-apoptotic factors and the calcium carbonate crystals inhibitor. Under physiological pH, Reg Iα becomes largely insoluble after a self-proteolysis process, and the N-terminally truncated form readily polymerizes into fibrils, which leads to neurodegenerative diseases. Reg Iα may form protofibril via lateral hydrophobic interactions with a native-like conformation. The structural basis from the native to fibril form, as well as the carbohydrate binding sites on Reg Iα, remain unknown. Here we present the NMR backbone and side-chain assignments of Reg Iα for use in further NMR investigations.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, 128 Section 2, Academia Road, Nankang, Taipei 115, Taiwan, ROC.
authors.forename: Meng-Ru
authors.initials: MR
authors.lastname: Ho
authors.forename: Chinpan
authors.initials: C
authors.lastname: Chen
mesh_terms: D002247:Carbon Isotopes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051847:Lithostathine / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011522:Protons
chemical_list: D002247:Carbon Isotopes; D051847:Lithostathine; D009587:Nitrogen Isotopes; D011522:Protons