title: Application of HR-MAS NMR spectroscopy for studying chemotype variations of Withania somnifera (L.) Dunal.
abstract: Withania somnifera (L.) Dunal (Solanaceae), commonly known as Ashwagandha, is one of the most valued Indian medicinal plants with a number of pharmaceutical and nutraceutical applications. Metabolic profiling has been performed by HR-MAS NMR spectroscopy on fresh leaf and root tissue specimens from four chemotypes of W. somnifera. The HR-MAS NMR spectroscopy of lyophilized defatted leaf tissue specimens clearly distinguishes resonances of medicinally important secondary metabolites (withaferin A and withanone) and its distinctive quantitative variability among the chemotypes. A total of 41 metabolites were identified from both the leaf and root tissues of the chemotypes. The presence of methanol in leaf and root tissues of W. somnifera was detected by HR-MAS NMR spectroscopy. Multivariate principal component analysis (PCA) on HR-MAS (1) H NMR spectra of leaves revealed clear variations in primary metabolites among the chemotypes. The results of the present study demonstrated an efficient method, which can be utilized for metabolite profiling of primary and secondary metabolites in medicinally important plants.
authors.affiliation: Centre of Biomedical Magnetic Resonance, SGPGIMS (Sanjay Gandhi Post-Graduate Institute of Medical Sciences) Campus, Lucknow, UP, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Bharti
authors.forename: Anil
authors.initials: A
authors.lastname: Bhatia
authors.forename: S K
authors.initials: SK
authors.lastname: Tewari
authors.forename: O P
authors.initials: OP
authors.lastname: Sidhu
authors.forename: Raja
authors.initials: R
authors.lastname: Roy
mesh_terms: D000432:Methanol / Q000737:chemistry; D015999:Multivariate Analysis; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000378:metabolism; D032341:Withania / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000432:Methanol