title: Profiling of immune-related microRNA expression in human cord blood and adult peripheral blood cells upon proinflammatory stimulation.
abstract: OBJECTIVE
  Cord blood (CB) transplantation has advantages in terms of incidence and severity of acute graft-versus-host disease (GVHD), while it has disadvantages in terms of infection. Our aim is to elucidate the molecular mechanism underlying the immune response of CB-derived cells during acute GVHD and infection following CB transplantation.
  
  
  METHODS
  We examined expression of 69 immune-relating microRNAs in CD4(+), CD8(+), and CD14(+) cells of CB and adult peripheral blood (APB) upon interferon-γ or lipopolysaccharide (LPS) stimulation.
  
  
  RESULTS
  Under basal condition, 20 microRNAs showed differential expression between CB and APB. Compared to APB counterparts, six microRNAs (miR-21, miR-22, miR-29a, miR-29b, miR-29c, and let-7c) were underexpressed in at least two cell lineages of CB, while five microRNAs (miR-15b, miR-181a, miR-181c, miR-363, and miR-424) were overexpressed in CD4(+) and CD8(+) CB cells. Upon interferon-γ stimulation, seven microRNAs (miR-29a, miR-29b, miR-34c-5p, miR-132, miR-146a, miR-146b-5p, and miR-155) changed in expression mainly in CD14(+) CB cells, while only two microRNAs (miR-18a and miR-155) changed in expression in CD14(+) CB cells upon LPS stimulation. These results suggest that the mechanisms regulating the expression of such immune-relating microRNAs in CD14(+) CB cells are much more sensitive to proinflammatory stimuli than those in APB CD14(+) cells, which might be related to the poor immunoreactivity of CD14(+) CB cells.
  
  
  CONCLUSIONS
  Our results suggest essential roles of specific microRNAs in regulating immune function of CB cells, providing insight into the underlying molecular mechanism.
authors.affiliation: Department of Advanced Medical Science, Institute of Medical Science, University of Tokyo, Tokyo, Japan. taka-ims@umin.ac.jp
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakaoka
authors.forename: Naohide
authors.initials: N
authors.lastname: Yamashita
mesh_terms: D000208:Acute Disease; D000328:Adult; D015703:Antigens, CD / Q000276:immunology* / Q000378:metabolism; D000998:Antiviral Agents / Q000494:pharmacology; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006086:Graft vs Host Disease / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D035683:MicroRNAs; D008875:Middle Aged; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD; D000998:Antiviral Agents; D008070:Lipopolysaccharides; D035683:MicroRNAs; D007371:Interferon-gamma