title: Epstein-Barr virus-induced gene 3 (EBI3): a novel diagnosis marker in Burkitt lymphoma and diffuse large B-cell lymphoma.
abstract: The distinction between Burkitt lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL), two types of mature aggressive B-cell lymphomas that require distinct treatments, can be difficult because of forms showing features intermediate between DLBCL and BL (here called BL/DLBCL). They can be discriminated by the presence of c-myc translocations characteristic of BL. However, these are not exclusive of BL and when present in DLBCL are associated with lower survival. In this study, we show that Epstein-Barr virus-induced gene 3 (EBI3) is differentially expressed among BL and DLBCL. Analysis of gene expression data from 502 cases of aggressive mature B-cell lymphomas available on Gene Expression Omnibus and immunohistochemical analysis of 184 cases of BL, BL/DLBCL or DLBCL, showed that EBI3 was not expressed in EBV-positive or -negative BL cases, whereas it was expressed by over 30% of tumoral cells in nearly 80% of DLBCL cases, independently of their subtypes. In addition, we show that c-myc overexpression represses EBI3 expression, and that DLBCL or BL/DLBCL cases with c-myc translocations have lower expression of EBI3. Thus, EBI3 immunohistochemistry could be useful to discriminate BL from DLBCL, and to identify cases of BL/DLBCL or DLBCL with potential c-myc translocations.
authors.affiliation: CNRS UMR 8147, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Gonin
authors.forename: Frédérique
authors.initials: F
authors.lastname: Larousserie
authors.forename: Christian
authors.initials: C
authors.lastname: Bastard
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Picquenot
authors.forename: Jérôme
authors.initials: J
authors.lastname: Couturier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Radford-Weiss
authors.forename: Céline
authors.initials: C
authors.lastname: Dietrich
authors.forename: Nicole
authors.initials: N
authors.lastname: Brousse
authors.forename: Marie-Cécile
authors.initials: MC
authors.lastname: Vacher-Lavenu
authors.forename: Odile
authors.initials: O
authors.lastname: Devergne
mesh_terms: D002051:Burkitt Lymphoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D007150:Immunohistochemistry; D007378:Interleukins / Q000235:genetics / Q000378:metabolism*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000378:metabolism*; D015778:Minor Histocompatibility Antigens; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C097732:EBI3 protein, human; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; D020598:Proto-Oncogene Proteins c-myb