title: Single nucleotide polymorphism (SNP) panels for rapid positional cloning in zebrafish.
abstract: Despite considerable genetic and genomic resources the positional cloning of forward mutations remains a slow and manually intensive task, typically using gel based genotyping and sequential rounds of mapping. We have used the latest genetic resources and genotyping technologies to develop two commercially available SNP panels of thousands of markers that can be used to speed up positional cloning.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Clark
authors.forename: Victor
authors.initials: V
authors.lastname: Guryev
authors.forename: Ewart de
authors.initials: Ed
authors.lastname: Bruijn
authors.forename: Isaac J
authors.initials: IJ
authors.lastname: Nijman
authors.forename: Masazumi
authors.initials: M
authors.lastname: Tada
authors.forename: Catherine
authors.initials: C
authors.lastname: Wilson
authors.forename: Panos
authors.initials: P
authors.lastname: Deloukas
authors.forename: John H
authors.initials: JH
authors.lastname: Postlethwait
authors.forename: Edwin
authors.initials: E
authors.lastname: Cuppen
authors.forename: Derek L
authors.initials: DL
authors.lastname: Stemple
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes / Q000235:genetics; D005820:Genetic Testing / Q000379:methods; D016678:Genome; D005838:Genotype; D007814:Larva; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D015027:Zebrafish / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004247:DNA