title: Effect of high pressure, in combination with antilisterial agents, on the growth of Listeria monocytogenes during extended storage of cooked chicken.
abstract: A cocktail of Listeria monocytogenes strains was inoculated into cooked chicken (∼2.2 × 10³ CFU g⁻¹) which was then pressure-treated (600 MPa/2 min/20 °C) and stored for up to 105 days at 8 °C. In addition, sodium lactate (2% w/w) or a pressure-resistant Weissella viridescens strain, known to have antilisterial activity, were added to the meat prior to inoculation with the pathogen and pressure treatment, to investigate the effect on Listeria survival. Pressure treatment alone was not sufficient to eliminate all of the Listeria. Numbers of survivors were initially below the level of detection (50 CFU g⁻¹) but increased during storage to reach >10⁸ CFU g⁻¹ by day 21. The presence of W. viridescens significantly extended the lag phase of any Listeria that survived the initial pressure treatment by ∼35 days, but numbers then increased to reach ∼10⁷ CFU g⁻¹ by day 105. The addition of 2% sodium lactate in combination with pressure treatment was most effective at inhibiting the growth of L. monocytogenes and numbers remained below the limit of detection throughout the 105 day storage. The addition of antimicrobial agents, in combination with pressure, could be used to give additional food safety assurance without increasing pressure hold time.
authors.affiliation: Food Microbiology Branch, Agriculture, Food and Environmental Science Division, Agri-Food and Biosciences Institute, Newforge Lane, Belfast BT95PX, Northern Ireland, UK. margaret.patterson@afbini.gov.uk
authors.forename: M F
authors.initials: MF
authors.lastname: Patterson
authors.forename: A
authors.initials: A
authors.lastname: Mackle
authors.forename: M
authors.initials: M
authors.lastname: Linton
mesh_terms: D000818:Animals; D000898:Antibiosis; D002645:Chickens; D003296:Cooking; D005511:Food Handling; D005519:Food Preservation / Q000295:instrumentation / Q000379:methods*; D005520:Food Preservatives / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D008460:Meat / Q000382:microbiology*; D050296:Microbial Viability; D011312:Pressure; D019354:Sodium Lactate / Q000494:pharmacology; D058836:Weissella / Q000502:physiology
chemical_list: D005520:Food Preservatives; D019354:Sodium Lactate