title: Th17 cytokines are critical for respiratory syncytial virus-associated airway hyperreponsiveness through regulation by complement C3a and tachykinins.
abstract: Respiratory syncytial virus (RSV) infection is associated with serious lung disease in infants and immunocompromised individuals and is linked to development of asthma. In mice, acute RSV infection causes airway hyperresponsiveness (AHR), inflammation, and mucus hypersecretion. Infected cells induce complement activation, producing the anaphylatoxin C3a. In this paper, we show RSV-infected wild-type mice produce Th17 cytokines, a response not previously associated with viral infections. Mice deficient in the C3aR fail to develop AHR following acute RSV infection, and production of Th17 cytokines was significantly attenuated. Tachykinin production also has been implicated in RSV pathophysiology, and tachykinin receptor-null mice were similarly protected from developing AHR. These animals were also deficient in production of Th17 cytokines. Tachykinin release was absent in mice deficient in C3aR, whereas C3a levels were unchanged in tachykinin receptor-null animals. Thus, our data reveal a crucial sequence following acute RSV infection where initial C3a production causes tachykinin release, followed by activation of the IL-17A pathway. Deficiency of either receptor affords protection from AHR, identifying two potential therapeutic targets.
authors.affiliation: Ina Sue Perlmutter Laboratory, Division of Respiratory Diseases, Department of Pediatrics, Children's Hospital, Boston, MA 02115, USA.
authors.forename: Monali M
authors.initials: MM
authors.lastname: Bera
authors.forename: Bao
authors.initials: B
authors.lastname: Lu
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Martin
authors.forename: Shun
authors.initials: S
authors.lastname: Cui
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Rhein
authors.forename: Craig
authors.initials: C
authors.lastname: Gerard
authors.forename: Norma P
authors.initials: NP
authors.lastname: Gerard
mesh_terms: D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D002469:Cell Separation; D015926:Complement C3a / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D020381:Interleukin-17 / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology* / Q000378:metabolism; D012136:Respiratory Syncytial Viruses / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015320:Tachykinins / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D015320:Tachykinins; D015926:Complement C3a