title: microRNA expression profiling in fetal single ventricle malformation             identified by deep sequencing.
abstract: microRNAs (miRNAs) have emerged as key regulators in many biological processes,             particularly cardiac growth and development, although the specific miRNA expression             profile associated with this process remains to be elucidated. This study aimed             to characterize the cellular microRNA profile involved in the development of congenital             heart malformation, through the investigation of single ventricle (SV) defects.             Comprehensive miRNA profiling in human fetal SV cardiac tissue was performed by             deep sequencing. Differential expression of 48 miRNAs was revealed by sequencing             by oligonucleotide ligation and detection (SOLiD) analysis. Of these, 38 were             down-regulated and 10 were up-regulated in differentiated SV cardiac tissue, compared             to control cardiac tissue. This was confirmed by real-time quantitative reverse             transcription-polymerase chain reaction (qRT-PCR) analysis. Predicted target genes             of the 48 differentially expressed miRNAs were analyzed by gene ontology and categorized             according to cellular process, regulation of biological process and metabolic             process. Pathway-Express analysis identified the WNT and mTOR signaling pathways             as the most significant processes putatively affected by the differential expression             of these miRNAs. The candidate genes involved in cardiac development were identified             as potential targets for these differentially expressed microRNAs and the collaborative             network of microRNAs and cardiac development related-mRNAs was constructed. These             data provide the basis for future investigation of the mechanism of the occurrence             and development of fetal SV malformations.
authors.affiliation: Department of Pediatrics, Nanjing Maternal and Child Health Hospital Affiliated of Nanjing Medical University, Nanjing, PR China.
authors.forename: Zhang-Bin
authors.initials: ZB
authors.lastname: Yu
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Han
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Bai
authors.forename: Chun
authors.initials: C
authors.lastname: Zhu
authors.forename: Ya
authors.initials: Y
authors.lastname: Pan
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D000022:Abortion, Spontaneous; D016022:Case-Control Studies; D016000:Cluster Analysis; D005333:Fetus; D020869:Gene Expression Profiling; D006321:Heart / Q000254:growth & development; D006330:Heart Defects, Congenital / Q000235:genetics* / Q000378:metabolism; D006352:Heart Ventricles / Q000002:abnormalities / Q000737:chemistry; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases; D051153:Wnt Proteins
chemical_list: D035683:MicroRNAs; D051153:Wnt Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases