title: Patterning human stem cells and endothelial cells with laser printing for cardiac regeneration.
abstract: Recent study showed that mesenchymal stem cells (MSC) could inhibit apoptosis of endothelial cells in hypoxic condition, increase their survival, and stimulate the angiogenesis process. In this project we applied Laser-Induced-Forward-Transfer (LIFT) cell printing technique and prepared a cardiac patch seeded with human umbilical vein endothelial cells (HUVEC) and human MSC (hMSC) in a defined pattern for cardiac regeneration. We seeded HUVEC and hMSC in a defined pattern on a Polyester urethane urea (PEUU) cardiac patch. On control patches an equal amount of cells was randomly seeded without LIFT. Patches were cultivated in vitro or transplanted in vivo to the infarcted zone of rat hearts after LAD-ligation. Cardiac performance was measured by left ventricular catheterization 8 weeks post infarction. Thereafter hearts were perfused with fluorescein tomato lectin for the assessment of functional blood vessels and stored for histology analyses. We demonstrated that LIFT-derived cell seeding pattern definitely modified growth characteristics of co-cultured HUVEC and hMSC leading to increased vessel formation and found significant functional improvement of infarcted hearts following transplantation of a LIFT-tissue engineered cardiac patch. Further, we could show enhanced capillary density and integration of human cells into the functionally connected vessels of murine vascular system. LIFT-based Tissue Engineering of cardiac patches for the treatment of myocardial infarction might improve wound healing and functional preservation.
authors.affiliation: Reference- and Translation Center for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University of Rostock, 18057 Rostock, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Gaebel
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Guan
authors.forename: Lothar
authors.initials: L
authors.lastname: Koch
authors.forename: Christian
authors.initials: C
authors.lastname: Klopsch
authors.forename: Martin
authors.initials: M
authors.lastname: Gruene
authors.forename: Anita
authors.initials: A
authors.lastname: Toelk
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.forename: Peter
authors.initials: P
authors.lastname: Mark
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Boris
authors.initials: B
authors.lastname: Chichkov
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.forename: Gustav
authors.initials: G
authors.lastname: Steinhoff
mesh_terms: D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D005355:Fibrosis; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006334:Heart Function Tests / Q000187:drug effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D020007:Implants, Experimental; D007834:Lasers; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009203:Myocardial Infarction / Q000473:pathology / Q000503:physiopathology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D012038:Regeneration / Q000187:drug effects / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014520:Urethane / Q000494:pharmacology
chemical_list: D014520:Urethane