title: Dynamics of phytohormone and DNA methylation patterns changes during dormancy induction in strawberry (Fragaria × ananassa Duch.).
abstract: Changes in endogenous phytohormone levels, DNA methylation patterns, and expression levels of related genes during induction of dormancy in two strawberry cultivars, Darselect and All Star, were studied under controlled environmental conditions. At 12°C, regardless of day length, potted, runner-derived plants of both cultivars gradually exhibited morphological traits typical of dormancy after treatment for 8 weeks. These morphological changes were accompanied by a synchronous significant decline in indole-3-acetic acid (IAA) level and increases in abscisic acid (ABA) content and global genomic DNA methylation in young leaves. Exposed at 15°C and a short-day photoperiod, the changes in morphology, phytohormone levels and DNA methylation of both cultivars were similar to those observed at 12°C. Slight but non-significant changes in IAA and ABA levels and genomic DNA methylation occurred in young leaves at both 15°C with long days and 18°C with short days. These results indicated that temperature alone was sufficient to induce strawberry to enter the typical dormant phase, and day length had no impact at 12°C. The higher temperature permissible for dormancy induction in strawberry was 15°C, but at this temperature dormancy induction was modified by day length. The expression patterns of FaPIN1, FaNCED1, FaDRM and FaROS1 were coincident with the changes in phytohormone levels and DNA methylation. Although the two tested cultivars have different temporal responses with the different degree of cold tolerance and depth of dormancy, both the endogenous phytohormone and DNA methylation were changed when induced by external environmental factors.
authors.affiliation: Institute for Horticultural Plants, China Agricultural University, Haidian District, Beijing, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Deguo
authors.initials: D
authors.lastname: Han
authors.forename: Changpeng
authors.initials: C
authors.lastname: Qiu
authors.forename: Zhenhai
authors.initials: Z
authors.lastname: Han
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D019175:DNA Methylation; D031985:Fragaria / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D017440:Photoperiod; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics; D013045:Species Specificity; D013696:Temperature
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; C030737:indoleacetic acid; D000040:Abscisic Acid