title: [Role of transcription factor special AT-rich binding protein 2 in the osteoblasts differentiation of bone marrow stromal cells].
abstract: OBJECTIVE
  To investigate the role of transcription factor special AT-rich binding protein 2 (SATB2) in the osteoblasts differentiation of bone marrow stromal cells (BMSC) in vitro.
  
  
  METHODS
  Rats bone marrow stromal cells were isolated by Percoll sedimentation and the cells were placed and allowed to attach for three times. After passages, expression plasmid pBABE-hygro-satb2 was constructed, then transfected into BMSC. BMSCs were inoculated in conditioned medium and osteogenic factors were detected by western blotting and reverse transcription polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The morphological observation of BMSC showed either spindle or polygonal pattern. The cellular phenotypic marker of the third passage was CD29 positive and CD34 negative. The growth curve possessed "S" pattern. The intensity of calfilication in BMSC was higher in SATB2 transfection group (IA value 125974 ± 241) than that in the control groups (IA value 178486 ± 406). Moreover, cell migration rate increased in SATB2 transfection group [width of scratch (0.72 ± 0.01) mm] compared with control group [width of scratch (0.83 ± 0.03) mm]. In addition, the mRNA expression of osteogenic factors runt-related transcription factor 2, Osterix, activating transcription factor 4, integrin-binding sialoprotein were upregulated.
  
  
  CONCLUSIONS
  Cells cultured with this method have general biological characteristics and osteogenic differentiation potential in vitro. SATB2 can promote osteoblasts differentiation of BMSC.
authors.affiliation: Department of Stomatology of Zhongshan Hospital, Fudan University, Shanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Yu
authors.forename: Zhang-Yu
authors.initials: ZY
authors.lastname: Gu
authors.forename: Wei
authors.initials: W
authors.lastname: Bi
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
mesh_terms: D051701:Activating Transcription Factor 4 / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D058574:Integrin-Binding Sialoprotein / Q000378:metabolism; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*; D018800:Thy-1 Antigens / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: C497110:Atf4 protein, rat; D050659:Core Binding Factor Alpha 1 Subunit; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D036961:Matrix Attachment Region Binding Proteins; D012333:RNA, Messenger; C493717:Runx2 protein, rat; C586446:SATB2 protein, rat; C495181:Sp7 protein, rat; D018800:Thy-1 Antigens; D014157:Transcription Factors; D051701:Activating Transcription Factor 4