title: Structure, stability, and mobility of a lyophilized IgG1 monoclonal antibody as determined using second-derivative infrared spectroscopy.
abstract: There are many aspects of stabilization of lyophilized proteins. Of these various factors, retention of native structure, having sufficient amount of stabilizer to embed the protein within an amorphous matrix, and dampening β-relaxations have been shown to be critical in optimizing protein stability during storage. In this study, an IgG1 was lyophilized with varying amounts of sucrose. In some formulations, a small amount of sorbitol was added as a plasticizer. The structure of the protein in dried state was monitored using infrared (IR) spectroscopy. The IR spectra indicated increasing retention of the native structure, which correlated with stability as indicated by size-exclusion chromatography as well as micro-flow imaging. Maximal stability was achieved with a 2:1 mass ratio of sucrose to protein, which is more than that would be expected based on earlier studies. Analysis of both high and low frequency bands associated with intramolecular β-sheet structure provides additional information on the structure of antibodies in the solid state. Finally, there is a correlation between the bandwidth of the β-sheet bands and the enthalpy of relaxation, suggesting that amide I bands can provide some indication of the degree of coupling to the sugar matrix, as well as structural heterogeneity of the protein.
authors.affiliation: Legacy BioDesign LLC, Johnstown, Colorado 80534, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Murphy
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Robert W
authors.initials: RW
authors.lastname: Payne
authors.forename: Janice M
authors.initials: JM
authors.lastname: Davis
authors.forename: Ahmad M
authors.initials: AM
authors.lastname: Abdul-Fattah
authors.forename: James E
authors.initials: JE
authors.lastname: Matsuura
authors.forename: Alan C
authors.initials: AC
authors.lastname: Herman
authors.forename: Mark Cornell
authors.initials: MC
authors.lastname: Manning
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004355:Drug Stability; D004356:Drug Storage; D005612:Freeze Drying / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry*; D055550:Protein Stability; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared / Q000379:methods; D013395:Sucrose / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013395:Sucrose