title: Chitosan nanoparticles affect the acid tolerance response in adhered cells of Streptococcus mutans.
abstract: In this study we evaluated the effect of chitosan nanoparticles on the acid tolerance response (ATR) of adhered Streptococcus mutans. An ATR was induced by exposing S. mutans to pH 5.5 for 2 h and confirmed by exposing the acid-adapted cells to pH 3.5 for 30 min, with the majority of cells appearing viable according to the LIVE/DEAD® technique. However, when chitosan nanoparticles were present during the exposure to pH 5.5, no ATR occurred as most cells appeared dead after the pH 3.5 shock. We conclude that the chitosan nanoparticles tested had the ability to hinder ATR induction in adhered S. mutans.
authors.affiliation: Department of Oral Biology, Faculty of Odontology, Malmö University, Malmö, Sweden. jessica.neilands@mah.se
authors.forename: J
authors.initials: J
authors.lastname: Neilands
authors.forename: D
authors.initials: D
authors.lastname: Sutherland
authors.forename: A
authors.initials: A
authors.lastname: Resin
authors.forename: P L
authors.initials: PL
authors.lastname: Wejse
authors.forename: L E
authors.initials: LE
authors.lastname: Chávez de Paz
mesh_terms: D000143:Acids / Q000378:metabolism; D000222:Adaptation, Physiological / Q000187:drug effects*; D001422:Bacterial Adhesion; D018441:Biofilms / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability; D053758:Nanoparticles; D013295:Streptococcus mutans / Q000187:drug effects* / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D000143:Acids; D048271:Chitosan