title: The PML nuclear bodies-associated protein TTRAP regulates ribosome biogenesis in nucleolar cavities upon proteasome inhibition.
abstract: TRAF and TNF receptor-associated protein (TTRAP) is a multifunctional protein that can act in the nucleus as a 5'-tyrosyl DNA phosphodiesterase and in the cytoplasm as a regulator of cell signaling. In this paper we show that in response to proteasome inhibition TTRAP accumulates in nucleolar cavities in a promyelocytic leukemia protein-dependent manner. In the nucleolus, TTRAP contributes to control levels of ribosomal RNA precursor and processing intermediates, and this phenotype is independent from its 5'-tyrosyl DNA phosphodiesterase activity. Our findings suggest a previously unidentified function for TTRAP and nucleolar cavities in ribosome biogenesis under stress.
authors.affiliation: Sector of Neurobiology, International School for Advanced Studies, Via Bonomea 265, Trieste, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Vilotti
authors.forename: M
authors.initials: M
authors.lastname: Biagioli
authors.forename: R
authors.initials: R
authors.lastname: Foti
authors.forename: M
authors.initials: M
authors.lastname: Dal Ferro
authors.forename: Z Scotto
authors.initials: ZS
authors.lastname: Lavina
authors.forename: L
authors.initials: L
authors.lastname: Collavin
authors.forename: G
authors.initials: G
authors.lastname: Del Sal
authors.forename: S
authors.initials: S
authors.lastname: Zucchelli
authors.forename: S
authors.initials: S
authors.lastname: Gustincich
mesh_terms: D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins; D057809:HEK293 Cells; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases; D000071156:Promyelocytic Leukemia Protein; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism; D061988:Proteasome Inhibitors; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology*; D012335:RNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013312:Stress, Physiological / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D000071156:Promyelocytic Leukemia Protein; D061988:Proteasome Inhibitors; D012335:RNA, Ribosomal; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C070576:PML protein, human; D010727:Phosphoric Diester Hydrolases; C474866:TDP2 protein, human; D046988:Proteasome Endopeptidase Complex