title: Proteomic analysis of polymorphonuclear neutrophils identifies catalase as a novel biomarker of abdominal aortic aneurysm: potential implication of oxidative stress in abdominal aortic aneurysm progression.
abstract: OBJECTIVE
  Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression. We have analyzed circulating PMNs isolated from AAA patients and controls by a proteomic approach to identify proteins potentially involved in AAA pathogenesis.
  
  
  RESULTS
  PMNs from 8 AAA patients (4 large AAA >5 cm and 4 small AAA 3-5 cm) and 4 controls were analyzed by 2D differential in-gel electrophoresis. Among differentially expressed spots, several proteins involved in redox balance were identified by mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase expression and activity were observed in PMNs from AAA patients compared with controls. In contrast, PMNs from AAA patients displayed higher H(2)O(2) and myeloperoxidase levels than PMNs from controls. Moreover, a significant decrease in catalase mRNA levels was observed in PMNs after phorbol 12-myristate 13-acetate incubation. Catalase plasma levels were also decreased in large (n=47) and small (n=56) AAA patients compared with controls (n=34). We observed catalase expression in AAA thrombus and thrombus-conditioned medium, associated with PMN infiltration. Furthermore, increased H(2)O(2) levels were observed in AAA thrombus-conditioned medium compared with the media layer.
  
  
  CONCLUSIONS
  Diminished catalase levels in circulating PMNs and plasma are observed in AAA patients, supporting an important role of oxidative stress in AAA evolution.
authors.affiliation: Vascular Research Laboratory, Fundación Jiménez Diaz-Instituto de Investigaciones Sanitarias, Autonoma University, Av Reyes Católicos 2, 28040 Madrid, Spain.
authors.forename: Priscila
authors.initials: P
authors.lastname: Ramos-Mozo
authors.forename: Julio
authors.initials: J
authors.lastname: Madrigal-Matute
authors.forename: Roxana
authors.initials: R
authors.lastname: Martinez-Pinna
authors.forename: Luis Miguel
authors.initials: LM
authors.lastname: Blanco-Colio
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Lopez
authors.forename: Emilio
authors.initials: E
authors.lastname: Camafeita
authors.forename: Olivier
authors.initials: O
authors.lastname: Meilhac
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Michel
authors.forename: Cesar
authors.initials: C
authors.lastname: Aparicio
authors.forename: Melina
authors.initials: M
authors.lastname: Vega de Ceniga
authors.forename: Jesus
authors.initials: J
authors.lastname: Egido
authors.forename: José Luis
authors.initials: JL
authors.lastname: Martín-Ventura
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017544:Aortic Aneurysm, Abdominal / Q000097:blood* / Q000503:physiopathology*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood*; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000097:blood; D040901:Proteomics
chemical_list: D015415:Biomarkers; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase