title: Silica-coated S(2-)-enriched manganese-doped ZnS quantum dots as a photoluminescence probe for imaging intracellular Zn2+ ions.
abstract: Detection of intracellular Zn(2+) has gained great attention because of its biological significances. Here we show the fabrication of silica-coated S(2-)-enriched Mn-doped ZnS quantum dots (SiO(2)-S-Mn-ZnS QDs) by enriching S(2-) with a silica shell on the surface of Mn-doped ZnS QDs via a sol-gel process for imaging intracellular Zn(2+) ions. The developed probe gave a good linearity for the calibration plot (the recovered PL intensity of the SiO(2)-S-Mn-ZnS QDs against the concentration of Zn(2+) from 0.3 to 15.0 μM), excellent reproducibility (1.2% relative standard deviation for 11 replicate measurements of Zn(2+) at 3 μM), and low detection limit (3s; 80 nM Zn(2+)). The SiO(2)-S-Mn-ZnS QDs showed negligible cytotoxicity, good sensitivity, and selectivity for Zn(2+) in a photoluminescence turn-on mode, being a promising probe for photoluminescence imaging of intracellular Zn(2+).
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, and State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, China.
authors.forename: Hu-Bo
authors.initials: HB
authors.lastname: Ren
authors.forename: Bo-Yue
authors.initials: BY
authors.lastname: Wu
authors.forename: Jia-Tong
authors.initials: JT
authors.lastname: Chen
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D049449:Luminescence; D008345:Manganese / Q000737:chemistry*; D015335:Molecular Probes; D010714:Phosphatidylethanolamines; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D015335:Molecular Probes; D010714:Phosphatidylethanolamines; D017967:Zinc Compounds; D008345:Manganese; D012822:Silicon Dioxide