title: Evaluation of Saccharomyces cerevisiae Y5 for ethanol production from enzymatic hydrolysate of non-detoxified steam-exploded corn stover.
abstract: Saccharomyces cerevisiae Y5 was used to produce ethanol from enzymatic hydrolysate of non-detoxified steam-exploded corn stover, with and without a nitrogen source, and decreasing inoculum size. The results indicated that the ethanol concentration of 44.55 g/L, corresponding to 94.5% of the theoretical yield was obtained after 24 h, with an inoculum size of 10% (v/v) and nitrogen source (corn steep liquor, CSL) of 40 mL/L. With the same inoculum size, and without CSL, the ethanol concentration was 43.21 g/L, corresponding to 91.7% of the theoretical value after 60 h. With a decreased inoculum size of 5% (v/v), and without CSL, the ethanol concentration was 40.00 g/L, corresponding to 85.8% of the theoretical value after 72 h. The strain offers the potential to improve the economy of cellulosic ethanol production by simplifying the production process and reducing the costs associated with the process such as water, capital equipment and nutrient supplementation.
authors.affiliation: College of Life Science, Capital Normal University, 100048 Beijing, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Gao
authors.forename: Shen
authors.initials: S
authors.lastname: Tian
authors.forename: Sijin
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiushan
authors.initials: X
authors.lastname: Yang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D009584:Nitrogen / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013227:Steam; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D004798:Enzymes; D013227:Steam; D014866:Waste Products; D000431:Ethanol; D009584:Nitrogen