title: A porous silicon optical microcavity for sensitive bacteria detection.
abstract: A porous silicon microcavity (PSM) is highly sensitive to subtle interface changes due to its high surface area, capillary condensation ability and a narrow resonance peak (∼10 nm). Based on the well-defined optical properties of a PSM, we successfully fabricated a bacteria detection chip for molecular or subcellular analysis by surface modification using undecylenic acid (UA), and the specific recognition binding of vancomycin to the D-alanyl-D-alanine of bacteria. The red shift of the PSM resonance peak showed a good linear relationship with bacteria concentration ranging from 100 to 1000 bacteria ml( - 1) at the level of relative standard deviation of 0.994 and detection limit of 20 bacteria ml( - 1). The resulting PSM sensors demonstrated high sensitivity, good reproducibility, fast response and low cost for biosensing.
authors.affiliation: CAS Key Lab of Health Informatics, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Huang
authors.forename: Lintao
authors.initials: L
authors.lastname: Cai
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D056656:Lab-On-A-Chip Devices; D055620:Optical Phenomena; D016062:Porosity; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*
chemical_list: D012825:Silicon