title: Mechanisms of glutamate efflux at the blood-brain barrier: involvement of glial cells.
abstract: At high concentrations, glutamate (Glu) exerts potent neurotoxic properties, leading to irreversible brain damages found in numerous neurological disorders. The accepted notion that Glu homeostasis in brain interstitial fluid is maintained primarily through the activity of Glu transporters present on glial cells does not take into account the possible contribution of endothelial cells constituting the blood-brain barrier (BBB) to this process. Here, we present evidence for the presence of the Glu transporters, excitatory amino-acid transporters (EAATs) 1 to 3, in porcine brain endothelial cells (PBECs) and show their participation in Glu uptake into PBECs. Moreover, transport of Glu across three in vitro models of the BBB is investigated for the first time, and evidence for Glu transport across the BBB in both directions is presented. Our results provide evidence that the BBB can function in the efflux mode to selectively remove Glu, via specific transporters, from the abluminal side (brain) into the luminal compartment (blood). Furthermore, we found that glial cells lining the BBB have an active role in the efflux process by taking up Glu and releasing it, through hemichannels, anion channels, and possibly the reversal of its EAATs, in close proximity to ECs, which in turn take up Glu and release it to the blood.
authors.affiliation: Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel. katayun.cohen-kashi@weizmann.ac.il
authors.forename: Katayun
authors.initials: K
authors.lastname: Cohen-Kashi-Malina
authors.forename: Itzik
authors.initials: I
authors.lastname: Cooper
authors.forename: Vivian I
authors.initials: VI
authors.lastname: Teichberg
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D050491:Glutamate Plasma Membrane Transport Proteins / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D006706:Homeostasis; D007150:Immunohistochemistry; D008954:Models, Biological; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013552:Swine
chemical_list: D050491:Glutamate Plasma Membrane Transport Proteins; D018698:Glutamic Acid