title: High-efficiency screening of monoclonal antibodies for membrane protein crystallography.
abstract: Determination of crystal structures of membrane proteins is often limited by difficulties obtaining crystals diffracting to high resolution. Co-crystallization with Fab fragments of monoclonal antibodies has been reported to improve diffraction of membrane proteins crystals. However, it is not simple to generate useful monoclonal antibodies for membrane protein crystallography. In this report, we present an optimized process for efficient screening from immunization to final validation of monoclonal antibody for membrane protein crystallography.
authors.affiliation: Howard Hughes Medical Institute, Department of Biochemistry, Brandeis University, Waltham, Massachusetts, United States of America. hhlim@brandeis.edu
authors.forename: Hyun-Ho
authors.initials: HH
authors.lastname: Lim
authors.forename: Yiling
authors.initials: Y
authors.lastname: Fang
authors.forename: Carole
authors.initials: C
authors.lastname: Williams
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism*; D002850:Chromatography, Gel; D018360:Crystallography, X-Ray / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D008565:Membrane Proteins