title: Establishment, characterization and viral susceptibility of a new cell line derived from goldfish, Carassius auratus (L.), tail fin.
abstract: A continuous cell line [goldfish tail fin (GFTF)] derived from a goldfish tail fin, Carassius auratus, was established and characterized. GFTF cells predominantly consist of fibroblast-like cells that were maintained and subcultured more than 50 times over a period of 15 months. Cells grew at temperatures between 15 and 37°C, with an optimum temperature of 25°C. The growth rate of GFTF cells increased proportionally with the foetal bovine serum (FBS) concentration (5-20%), with optimum growth at 20% FBS. The chromosome numbers were 88-112, with a modal peak of 104 chromosomes. Five known fish viruses were tested to determine susceptibility. Results demonstrated that GFTF is susceptible to snakehead rhabdovirus, spring viraemia of carp virus and channel catfish virus (CCV). In addition, GFTF demonstrated a higher sensitivity to, and increased viral production of, CCV than that observed in the control cell line, channel catfish ovary cells. This suggests that GFTF cells would be useful as a diagnostic tool for viral diseases in this fish species, as well as for the isolation and study of goldfish viruses in the future. Furthermore, these cells were transfected with pEGFP-N1 vector DNA and some fluorescent signals were observed, suggesting that GFTF cells could be a useful tool for transgenic and genetic manipulation studies.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province, China.
authors.forename: W
authors.initials: W
authors.lastname: Yan
authors.forename: P
authors.initials: P
authors.lastname: Nie
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
mesh_terms: D058500:Animal Fins / Q000166:cytology*; D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D015925:Cryopreservation; D004198:Disease Susceptibility; D015870:Gene Expression; D006054:Goldfish / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D059785:Karyotype; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013696:Temperature; D014162:Transfection; D010948:Viral Plaque Assay; D014777:Virus Diseases / Q000662:veterinary*; D018406:Virus Physiological Phenomena; D014779:Virus Replication
chemical_list: D012336:RNA, Ribosomal, 16S; D012337:RNA, Ribosomal, 18S; D049452:Green Fluorescent Proteins