title: A genetic comparison of pig, cow and trout isolates of Lactococcus garvieae by PFGE analysis.
abstract: OBJECTIVE
  Genetic comparison of Lactococcus garvieae isolated from mammals and fish.
  
  
  RESULTS
  One hundred and ninety-seven L. garvieae isolates obtained from trout (n = 153), cow (n = 7) and pigs (n = 37) were genetically characterized by determining their pulsed-field gel electrophoresis (PFGE) profiles after macrorestriction with Bsp120I. Overall, L. garvieae isolates from pigs, cow and trout exhibited distinct PFGE patterns, with a low genetic relationship between them. Isolates from trout generated two pulsotypes [Genetic diversity (GD) 0.01] showing that the fish isolates were more genetically homogenous than the others. The L. garvieae isolates from cows displayed five (GD 0.71) different pulsotypes, while the swine isolates displayed 13 different pulsotypes (GD 0.35). Twenty-one of the 37 swine strains (56.8%) were grouped in a single cluster that included two closely related (93% similarity) pulsotypes. These pulsotypes exhibited a high frequency of isolation from different organs of the animals, and they were also broadly distributed among herds, suggesting a wide distribution across the swine population. This suggests that L. garvieae might be able to colonize different organs of the swine cardio-respiratory system.
  
  
  CONCLUSIONS
  Results indicate that most L. garvieae isolates from pigs and trout exhibited a distinct genetic background.
  
  
  CONCLUSIONS
  The present study describes the isolation of L. garvieae from both diseased and healthy pigs for the first time, and the findings suggest that pigs could be a previously unknown reservoir of this pathogen.
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Tejedor
authors.forename: A I
authors.initials: AI
authors.lastname: Vela
authors.forename: A
authors.initials: A
authors.lastname: Gibello
authors.forename: A
authors.initials: A
authors.lastname: Casamayor
authors.forename: L
authors.initials: L
authors.lastname: Domínguez
authors.forename: J F
authors.initials: JF
authors.lastname: Fernández-Garayzábal
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005393:Fish Diseases / Q000382:microbiology*; D005506:Food Contamination; D014644:Genetic Variation; D017045:Lactococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008413:Mastitis / Q000382:microbiology / Q000662:veterinary*; D008974:Mollusca / Q000382:microbiology; D010802:Phylogeny; D017747:Seafood / Q000382:microbiology; D034421:Sus scrofa / Q000382:microbiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014337:Trout