title: Prevalence and different characteristics of two serotypes of Streptococcus parauberis isolated from the farmed olive flounder, Paralichthys olivaceus (Temminck and Schlegel), in Korea.
abstract: The prevalence of two serotypes of Streptococcus parauberis isolated from the olive flounder, Paralichthys olivaceus, was evaluated in a total of 29 isolates between 2003 and 2010 in Korea. Streptococcus parauberis isolates were divided into two serologically distinct types (serotype 1 and serotype 2), except for one strain (S1091), using an agglutination assay with rabbit antiserum, and serotype 1 was identified as the dominant type (24 of 29 isolates) in this study. To identify the characteristics of the two serotypes of S. parauberis, we conducted a biochemical test using the API 20 Strep kit, a transmission electron microscopy (TEM) assay, sequence analysis of 16S-23S rRNA intergenic spacer region (ISR) and a pathogenicity test. In TEM, both serotypes possessed polysaccharide capsule layers around the cell surface when bacterial cells were treated with a homologous serotype of rabbit antiserum. However, we were unable to discriminate serotype-specific biochemical characteristics and genetic characteristics of 16S-23S rRNA ISR between the two serotypes. In the pathogenicity test, the serotype 1 strains induced significantly higher mortality than the serotype 2 strains in olive flounder when experimentally inoculated via the intraperitoneal route.
authors.affiliation: Research Unit, Green Cross Veterinary Product, Yongin, Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Han
authors.forename: B K
authors.initials: BK
authors.lastname: Kang
authors.forename: B J
authors.initials: BJ
authors.lastname: Kang
authors.forename: S P
authors.initials: SP
authors.lastname: Shin
authors.forename: B H
authors.initials: BH
authors.lastname: Soen
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Choresca
authors.forename: J E
authors.initials: JE
authors.lastname: Han
authors.forename: J W
authors.initials: JW
authors.lastname: Jun
authors.forename: S C
authors.initials: SC
authors.lastname: Park
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000382:microbiology* / Q000401:mortality; D005432:Flounder; D007723:Korea / Q000453:epidemiology; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015995:Prevalence; D016415:Sequence Alignment; D012703:Serotyping; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000401:mortality / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer