title: Targeted novel surface-modified nanoparticles for interferon delivery for the treatment of hepatitis B.
abstract: The purpose of the present work was to develop hepatitis B surface antigen (HBsAg) surface-adsorbed cationic poly (d,l-lactic-co-glycolic acid) PLGA nanoparticles for interferon alpha (IFNα) delivery targeted to hepatocytes. Cationic PLGA nanoparticles loaded with IFNα were prepared using the double emulsification technique. Delipidated HBsAg was passively adsorbed on the surface of nanoparticles by using the simple dipping and drying method. Surface morphology and size distribution of nanoparticles were analyzed by scanning electron microscopy and dynamic light-scattering method, respectively. The biodistribution behavior of plain and HBsAg-coated (99m)Tc-tagged PLGA nanoparticles was also examined followed by intravenous injection. The results revealed that ∼75% of the radioactivity was recovered in the liver after 4 h of injection that was nearly 3-fold greater in magnitude than the plain PLGA nanoparticles. These data demonstrated that the novel formulation of nanoparticles has potential application in hepatic-targeted drug delivery.
authors.affiliation: Department of Pharmaceutical Sciences,  Dr Hari Singh Gour Vishwavidyalaya, Sagar, MP 470003,  India.
authors.forename: Namita
authors.initials: N
authors.lastname: Giri
authors.forename: Priti
authors.initials: P
authors.lastname: Tomar
authors.forename: Vijai Singh
authors.initials: VS
authors.lastname: Karwasara
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Pandey
authors.forename: V K
authors.initials: VK
authors.lastname: Dixit
mesh_terms: D000818:Animals; D016503:Drug Delivery Systems; D005260:Female; D006509:Hepatitis B / Q000188:drug therapy*; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage / Q000737:chemistry*; D006801:Humans; D016898:Interferon-alpha / Q000008:administration & dosage* / Q000737:chemistry* / Q000627:therapeutic use; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D008099:Liver / Q000737:chemistry / Q000000981:diagnostic imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000648:ultrastructure; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011877:Radionuclide Imaging; D013667:Technetium / Q000008:administration & dosage / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D006514:Hepatitis B Surface Antigens; D016898:Interferon-alpha; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013667:Technetium