title: Usefulness of three-channel multiplex real-time PCR and melting curve analysis for simultaneous detection and identification of the Mycobacterium tuberculosis complex and nontuberculous mycobacteria.
abstract: We attempted to determine the benefits of three-channel multiplex real-time PCR and melting curve analysis not only in detecting and distinguishing between nontuberculous mycobacteria (NTM) and the Mycobacterium tuberculosis complex but also in identifying NTM to the species level.
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, South Korea.
authors.forename: Yun Ji
authors.initials: YJ
authors.lastname: Hong
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Chung
authors.forename: Taek Soo
authors.initials: TS
authors.lastname: Kim
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Song
authors.forename: Kyoung Un
authors.initials: KU
authors.lastname: Park
authors.forename: Jae Joon
authors.initials: JJ
authors.lastname: Yim
authors.forename: Junghan
authors.initials: J
authors.lastname: Song
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Eui Chong
authors.initials: EC
authors.lastname: Kim
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009164:Mycobacterium Infections / Q000175:diagnosis* / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009170:Nontuberculous Mycobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D044366:Transition Temperature