title: Bioreactor optimization of tissue engineered rabbit flexor tendons in vivo.
abstract: Tissue-engineered rabbit flexor tendons reseeded with cells are stronger in vitro after culture in a bioreactor. It is not known whether this effect persists in vivo. Tenocytes from New Zealand white rabbits were seeded onto rabbit rear paw flexor tendons that were deprived of cells and exposed to cyclic strain in a bioreactor. Reseeded constructs that were kept unloaded in a medium for 5 days were used as controls. The tendons were implanted to bridge a zone II defect in the rabbit. After explantation 4 weeks later, the ultimate tensile strength (UTS) and elastic modulus (EM) were determined. Tendon constructs that were exposed to cyclic strain had significantly improved UTS and EM. Histology showed that cellularity was increased in the bioreactor tendons.
authors.affiliation: Section of Plastic Surgery, Department of Veterans Affairs and Division of Plastic Surgery, Stanford University Medical Center, Stanford, CA, USA. johan@thorfinn.se
authors.forename: J
authors.initials: J
authors.lastname: Thorfinn
authors.forename: I K
authors.initials: IK
authors.lastname: Angelidis
authors.forename: L
authors.initials: L
authors.lastname: Gigliello
authors.forename: H M
authors.initials: HM
authors.lastname: Pham
authors.forename: D
authors.initials: D
authors.lastname: Lindsey
authors.forename: J
authors.initials: J
authors.lastname: Chang
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena / Q000502:physiology; D019149:Bioreactors; D004548:Elasticity / Q000502:physiology*; D008297:Male; D011817:Rabbits; D013710:Tendons / Q000166:cytology* / Q000503:physiopathology*; D013718:Tensile Strength / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D016474:Weight-Bearing / Q000502:physiology