title: Lipoxygenase inhibitory activity of crude bark extracts and isolated compounds from Commiphora berryi.
abstract: BACKGROUND
  Commiphora berryi is traditionally used for the treatment of cold and fever as well as for wound healing in the southern parts of India.
  
  
  OBJECTIVE
  The present study was designed to investigate in vitro soybean lipoxygenase inhibitory activity of crude extracts and compounds isolated from Commiphora berryi.
  
  
  METHODS
  The bark of Commiphora berryi was extracted with different organic solvents and subjected to chromatographic separation for isolation of bioactive compounds. Structures of isolated compounds were elucidated by spectroscopic methods. The anti-inflammatory activity of bark extracts and bioactive compounds were assessed by in vitro soybean lipoxygenase (SBL) assay.
  
  
  RESULTS
  3β-Hydroxyglutin-5-ene (1), friedelin (2), cycloeucaneol (3) nimbiol (4), sugiol (5), surianol (6), daucosterol (7) and ursolic acid (8) were isolated from crude bark extracts of the Commiphora berryi. The structure of nimbiol (4) was also confirmed by single crystal X-ray analysis. The petroleum ether, methanol, chloroform and ethyl acetate extracts of bark of Commiphora berryi showed SBL inhibitory activity with the IC(50) values of 15.3, 54.2, 71.5 and 87.8 μg/ml respectively. Among all the isolates, friedelin (2) showed significant SBL inhibitory activity with IC(50) 35.8 μM.
  
  
  CONCLUSIONS
  The overall results provide evidence that the studied plant might be a potential source of anti-inflammatory agents.
authors.affiliation: Department of Medicinal Chemistry, CSIR-Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Jadavpur, Calcutta 700032, India.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Kumari
authors.forename: Arumugam
authors.initials: A
authors.lastname: Meyyappan
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Selvamani
authors.forename: Joydeep
authors.initials: J
authors.lastname: Mukherjee
authors.forename: Parasuraman
authors.initials: P
authors.lastname: Jaisankar
mesh_terms: D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D029744:Commiphora / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D008084:Lipoxygenase / Q000378:metabolism*; D015394:Molecular Structure; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013025:Glycine max / Q000201:enzymology; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000094042:Lupanes
chemical_list: D000893:Anti-Inflammatory Agents; D008084:Lipoxygenase; D010936:Plant Extracts; D014315:Triterpenes; C060796:friedelin; D000094042:Lupanes