title: Analysis of single-nucleotide polymorphisms in patient-derived retrovirus integration sites reveals contamination from cell lines acutely infected by xenotropic murine leukemia virus-related virus.
abstract: We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical to those in cell lines but not the patients, whereas the data on the remaining 12 integration sites were inconclusive. Our results provide direct evidence for contamination during analysis of XMRV integration sites.
authors.affiliation: Department of Molecular and Medical Pharmacology, UCLA AIDS Institute, UCLA School of Medicine, Los Angeles, CA 90095, USA.
authors.forename: Alice
authors.initials: A
authors.lastname: Rusmevichientong
authors.forename: Jaydip
authors.initials: J
authors.lastname: Das Gupta
authors.forename: Petra S
authors.initials: PS
authors.lastname: Elias
authors.forename: Robert H
authors.initials: RH
authors.lastname: Silverman
authors.forename: Samson A
authors.initials: SA
authors.lastname: Chow
mesh_terms: D000818:Animals; D001483:Base Sequence; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000821:virology*; D011533:Proviruses / Q000235:genetics; D012192:Retroviridae Infections / Q000235:genetics / Q000821:virology*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D016662:Virus Integration / Q000235:genetics*; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity