title: Identification of avocado (Persea americana) root proteins induced by infection with the oomycete Phytophthora cinnamomi using a proteomic approach.
abstract: Avocado root rot, caused by Phytophthora cinnamomi, is the most important disease that limits avocado production. A proteomic approach was employed to identify proteins that are upregulated by infection with P. cinnamomi. Different proteins were shown to be differentially expressed after challenge with the pathogen by two-dimensional (2-D) gel electrophoresis. A densitometric evaluation of protein expression indicated differential regulation during the time-course analyzed. Some proteins induced in response to the infection were identified by standard peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry and sequencing by MALDI LIFT-TOF/TOF tandem mass spectrometry. Of the 400 protein spots detected on 2-D gels, 21 seemed to change in abundance by 3 hours after infection. Sixteen proteins were upregulated, 5 of these were only detected in infected roots and 11 showed an increased abundance. Among the differentially expressed proteins identified are homologs to isoflavone reductase, glutathione S-transferase, several abscisic acid stress-ripening proteins, cinnamyl alcohol dehydrogenase, cinnamoyl-CoA reductase, cysteine synthase and quinone reductase. A 17.3-kDa small heat-shock protein and a glycine-rich RNA-binding protein were identified as downregulated. Our group is the first to report on gene induction in response to oomycete infection in roots from avocado, using proteomic techniques.
authors.affiliation: Centro de Investigación en alimentación y desarrollo A C, Av. Rio Conchos s/n, Parque Industrial Cuauhtémoc, CP. 31570, Cuauhtémoc, Chihuahua, México.
authors.forename: Carlos H
authors.initials: CH
authors.lastname: Acosta-Muñiz
authors.forename: Lina
authors.initials: L
authors.lastname: Escobar-Tovar
authors.forename: Silvia
authors.initials: S
authors.lastname: Valdes-Rodríguez
authors.forename: Silvia
authors.initials: S
authors.lastname: Fernández-Pavia
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Arias-Saucedo
authors.forename: Maria
authors.initials: M
authors.lastname: de la Cruz Espindola Barquera
authors.forename: Miguel Á
authors.initials: MÁ
authors.lastname: Gómez Lim
mesh_terms: D060467:Disease Resistance; D054884:Host-Pathogen Interactions; D027421:Persea / Q000378:metabolism / Q000469:parasitology*; D010838:Phytophthora / Q000254:growth & development*; D010935:Plant Diseases / Q000469:parasitology*; D010940:Plant Proteins / Q000032:analysis / Q000096:biosynthesis*; D018517:Plant Roots / Q000378:metabolism / Q000469:parasitology; D040901:Proteomics / Q000379:methods
chemical_list: D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant