title: [Effects of hot shock protein 70 inhibitor PFTµ on inflammatory response in lipopolysaccharide-stimulated RAW264.7 cells and mice underwent myocardial ischemia-reperfusion injury].
abstract: OBJECTIVE
  To observe the effects of hot shock protein 70 (HSP70) inhibitor (PFTµ) on inflammation response in lipopolysaccharide (LPS)-stimulated RAW264.7 cells and mice underwent myocardial ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  RAW264.7 macrophage line of mice was stimulated by LPS as an inflammatory model. These were divided into control (15 min DMSO pretreatment and LPS 2 g/L) and PFTµ treated groups (15 min PFTµ 20 µmol/L pretreatment and LPS 2 g/L). NO concentration was measured by Griess Kit. The expression of iNOS protein and mRNA were detected by Western blot and RT-PCR. Infarct size was determined on mice underwent myocardial ischemia-reperfusion (I/R) injury in the absence or presence (PFTµ 40 mg/kg, intraperitoneal injection).
  
  
  RESULTS
  PFTµ significantly blocked the production of NO and protein and mRNA expression of iNOS (P < 0.05 vs. control). PFTµ also significantly reduced the infarct size on mice underwent I/R injury (P < 0.05 vs. control).
  
  
  CONCLUSIONS
  These results suggest that PFTµ could be a potential therapeutic agent for the treatment of inflammatory diseases through inhibiting the production of NO and reducing inflammatory responses.
authors.affiliation: Department of Cardiology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Yuan
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Xia
authors.forename: Kang-Hua
authors.initials: KH
authors.lastname: Ma
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000494:pharmacology*; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors; D007249:Inflammation; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D014050:Toluene / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D052160:Benzothiazoles; D018840:HSP70 Heat-Shock Proteins; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D014050:Toluene; C121565:pifithrin; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse