title: Amplification strategy using aggregates of ferrocene-containing cationic polythiophene for sensitive and specific electrochemical detection of DNA.
abstract: We report a new electrochemical amplification strategy for an ultrasensitive electrochemical detection of DNA sequences using aggregates composed of a water-soluble, ferrocene-functionalized polythiophene. A two-step hybridization is performed at one addressing surface with PNA capture probes whereas the electrochemical detection is done on an electrode nearby. Specific and quantitative detection of DNA targets with a detection limit of 4 × 10(-16) M (about 4 zeptomoles or about 2500 copies of oligonucleotides) was achieved.
authors.affiliation: Canada Research Chair on Electroactive and Photoactive Polymers, Département de Chimie, Université Laval, Québec City, Québec, Canada.
authors.forename: Patricia Harding
authors.initials: PH
authors.lastname: Lepage
authors.forename: Régis
authors.initials: R
authors.lastname: Peytavi
authors.forename: Michel G
authors.initials: MG
authors.lastname: Bergeron
authors.forename: Mario
authors.initials: M
authors.lastname: Leclerc
mesh_terms: D015374:Biosensing Techniques; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D000075163:Metallocenes; D049329:Nanostructures; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D002412:Cations; D015342:DNA Probes; D005296:Ferrous Compounds; D000075163:Metallocenes; D020135:Peptide Nucleic Acids; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D006046:Gold; D004247:DNA; C004998:ferrocene