title: Chromatin immunoprecipitation in adult zebrafish red cells.
abstract: Zebrafish has been used for many years as a model to study development and disease. The ability of zebrafish to produce thousand of embryos in a synchronous manner has made zebrafish an invaluable tool for genetic and chemical screens. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited. In this chapter, we describe a simple method to identify DNA binding sites and chromatin architecture in erythrocytes from adult zebrafish using chromatin immunoprecipitation coupled with next generation sequencing. This technique has been used extensively and successfully in other systems and it will be a useful tool for studying epigenetics in zebrafish.
authors.affiliation: Stem Cell Program, Children's Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts, USA.
authors.forename: Eirini
authors.initials: E
authors.lastname: Trompouki
authors.forename: Teresa V
authors.initials: TV
authors.lastname: Bowman
authors.forename: Anthony
authors.initials: A
authors.lastname: Dibiase
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Leonard I
authors.initials: LI
authors.lastname: Zon
mesh_terms: D000818:Animals; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D044127:Epigenesis, Genetic; D004912:Erythrocytes / Q000378:metabolism; D005557:Formaldehyde / Q000737:chemistry; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006657:Histones / Q000378:metabolism; D011485:Protein Binding; D017422:Sequence Analysis, DNA / Q000379:methods; D013010:Sonication / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D014157:Transcription Factors / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics*
chemical_list: D002843:Chromatin; D003432:Cross-Linking Reagents; D006657:Histones; D014157:Transcription Factors; D005557:Formaldehyde; D004247:DNA