title: Effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned buffalo (Bubalus bubalis) embryos.
abstract: The aim of the present study was to investigate the effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned (HMC) and parthenogenetically activated (PA) buffalo embryos. Scriptaid treatment was given for 10 h immediately after reconstruction of HMC and PA embryos. Treatment of HMC embryos with 500 or 1000 nmol/L scriptaid significantly increased (P<0.01) the cleavage rate (91.3±2.8 and 91.9±2.0%, respectively) than that of controls (80.7±2.4%). Blastocyst rate was significantly higher (P<0.01) following treatment with 1000 nmol/L (54.1±5.0%) than that with 500 nmol/L scriptaid (42.6±2.9%) which, in turn, was higher (P<0.01) than that of the controls (38.0±2.6%). Also, HMC embryos treated with 500 or 1000 nmol/L scriptaid had higher (P<0.01) cell number (339.9±1.4 and 343.4±2.4, respectively) than the untreated embryos (150.7±2.0). In contrast, in zona-free PA embryos, the cleavage and blastocyst rate was significantly lower (P<0.01) following treatment with 500 (46.3±1.0 and 22.0±1.4%, respectively) or 1000 nmol/L scriptaid (46.2±1.9 and 16.6±1.3%, respectively) than that of untreated embryos (60.4±3.2 and 29.5±2.1%, respectively). Transfer of HMC embryos that had been untreated (N=13) or treated with 1000 nmol/L scriptaid (N=15) to synchronized recipients resulted in one pregnancy each. In conclusion, these results demonstrate that scriptaid treatment improves the developmental potential of HMC buffalo embryos, but compromises that of zona-free PA embryos.
authors.affiliation: Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Panda
authors.forename: A
authors.initials: A
authors.lastname: George
authors.forename: A
authors.initials: A
authors.lastname: Saha
authors.forename: R
authors.initials: R
authors.lastname: Sharma
authors.forename: A K
authors.initials: AK
authors.lastname: Singh
authors.forename: R S
authors.initials: RS
authors.lastname: Manik
authors.forename: M S
authors.initials: MS
authors.lastname: Chauhan
authors.forename: P
authors.initials: P
authors.lastname: Palta
authors.forename: S K
authors.initials: SK
authors.lastname: Singla
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000196:embryology*; D019976:Cloning, Organism / Q000662:veterinary; D046149:Embryo Culture Techniques / Q000662:veterinary*; D004624:Embryo Transfer / Q000662:veterinary; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology*; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D011247:Pregnancy; D011804:Quinolines / Q000494:pharmacology*; D015044:Zona Pellucida / Q000502:physiology
chemical_list: D056572:Histone Deacetylase Inhibitors; D006898:Hydroxylamines; D011804:Quinolines; C410733:scriptaid