title: Differential role of YiGanKang decoction in IL-1β induction of IL-1RI and AP-1 in rat hepatic stellate cell.
abstract: OBJECTIVE
  YiGanKang, a combination of Chinese herbs, has anti-fibrosis effects in chronic liver diseases. The present study tries to demonstrate differential role of YiGanKang Decoction in interleukin-1β (IL-1β) induction of the type I receptor (IL-1RI) and the activator protein 1 (AP-1) in rat hepatic stellate cell (HSC).
  
  
  METHODS
  Flow cytometry was used to detect the IL-1RI expression. Electrophoretic mobility shift assays was used to detect AP-1 activity.
  
  
  RESULTS
  IL-1RI expression after IL-1β treatment for 0, 2, 10, 60, and 120 min was 227.08 (13.15), 268.43 (8.93), 442.97 (7.00), 367.66 (14.70), and 261.58 (15.08), respectively. The differences between IL-1RI expression after treatment for 10 and 60 min were significantly higher than the corresponding values in the control (P<0.01, P<0.01, respectively); After pretreatment with YiGanKang Decoction, IL-1RI expression induced by IL-1β was not decrease obviously; IL-1β could activate AP-1 in rat HSCs (P<0.01). Meanwhile YiGanKang Decoction could inhibit activity of AP-1 induced by IL-1β (P<0.01), and the inhibition rate was 42.71%.
  
  
  CONCLUSIONS
  YiGanKang Decoction could not decrease IL-1RI expression, but it could inhibit activity of AP-1 in rat HSCs induced by IL-1β.
authors.affiliation: Department of Pediatrics, The Third Hospital of Hebei Medical University, Shijiazhuang 050051, China. gracezhangyaping@yahoo.com.cn
authors.forename: Yaping
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ai
authors.forename: Junxia
authors.initials: J
authors.lastname: Zhang
authors.forename: Xixian
authors.initials: X
authors.lastname: Yao
mesh_terms: D000818:Animals; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D024202:Electrophoretic Mobility Shift Assay; D005434:Flow Cytometry; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D010946:Plants, Medicinal; D051381:Rats; D053573:Receptors, Interleukin-1 Type I / Q000378:metabolism*; D013997:Time Factors; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D053573:Receptors, Interleukin-1 Type I; D018808:Transcription Factor AP-1; C560535:YiGanKang