title: Surface-assisted laser desorption and ionization mass spectrometry using low-cost matrix-free substrates.
abstract: Surface-assisted laser desorption/ionization (SALDI) substrates have been fabricated using nanospiked polyurethane (PU) substrates that are replicated by a low-cost soft nanolithography method from silicon nanospike structures formed with femtosecond laser irradiations. The strongest mass spectrometry (MS) signal of Angiotensin II was obtained on 45-nm Au-coated nanospiked PU substrates. The effective ionization appears to be due to surface plasmon excitation. Such low-cost and identical SALDI substrates can be used for MS analysis of various molecules with high reproducibility.
authors.affiliation: Department of Physics and Applied Physics, and Nanomanufacturing Center, University of Massachusetts Lowell, 1 University Avenue, Lowell, MA 01854, USA.
authors.forename: Haibin
authors.initials: H
authors.lastname: Huo
authors.forename: Mengyan
authors.initials: M
authors.lastname: Shen
authors.forename: Steven M
authors.initials: SM
authors.lastname: Ebstein
authors.forename: Howard
authors.initials: H
authors.lastname: Guthermann
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000804:Angiotensin II / Q000032:analysis; D006046:Gold / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology; D011140:Polyurethanes / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000379:methods*; D020349:Surface Plasmon Resonance
chemical_list: D011140:Polyurethanes; D000804:Angiotensin II; D006046:Gold; D000324:Adrenocorticotropic Hormone