title: Detecting DNaseI-hypersensitivity sites with MLPA.
abstract: DNaseI-hypersensitive sites within chromatin are indicative of genomic loci with regulatory function. Several techniques have been described for analyzing these regions, but are either laborious, offer low-throughput possibilities, or are expensive. We have developed a new approach based on a modified version of multiplex ligation-dependent probe amplification (MLPA). Using this method, it is possible to analyse up to 50 defined genomic regions for DNaseI-hypersensitivity in a single PCR-based reaction. This chapter outlines the approach and discusses the critical features of each step of the procedure.
authors.affiliation: Molecular Development Laboratory, Murdoch Childrens Research Institute, Royal Children's Hospital, Melbourne, VIC, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ohnesorg
authors.forename: Stefanie
authors.initials: S
authors.lastname: Eggers
authors.forename: Stefan J
authors.initials: SJ
authors.lastname: White
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002843:Chromatin / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D016678:Genome / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
chemical_list: D002843:Chromatin; D003850:Deoxyribonuclease I