title: AFM membrane roughness as a probe to identify oxidative stress-induced cellular apoptosis.
abstract: The morphological change of cellular apoptosis initiates from the change of membrane roughness. In order to identify cellular apoptosis in its early stage, atomic force microscope was adapted to reveal the change of membrane roughness in unprecedented details, providing an image in nanometer-scaled resolution. The mouse monocyte/macrophage cell line RAW 264.7 was the subject studied and subjected to apoptotic induction by hydrogen peroxide. A finding of the qualitative correlation between cell membrane roughness and oxidative stress level is disclosed stating that roughness is increasing with the increasing level of oxidative stress.
authors.affiliation: Department of Mechanical Engineering, National Yunlin University of Science and Technology, Taiwan. wangdc@yuntech.edu.tw
authors.forename: Dau-Chung
authors.initials: DC
authors.lastname: Wang
authors.forename: Ken-Yen
authors.initials: KY
authors.lastname: Chen
authors.forename: Cheng-Hsien
authors.initials: CH
authors.lastname: Tsai
authors.forename: Gen-You
authors.initials: GY
authors.lastname: Chen
authors.forename: Chuan-Hung
authors.initials: CH
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008264:Macrophages / Q000648:ultrastructure*; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D013499:Surface Properties
chemical_list: D016877:Oxidants; D006861:Hydrogen Peroxide