title: Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper.
abstract: The construction of a normalized cDNA library is a popular tool for identifying novel biomarkers for monitoring environmental pollution. In the present study, a normalized cDNA library was constructed from the river snail Bellamya aeruginosa after exposure to Cu(2+) by using the SMART technique. The titer of the cDNA library was 1.78 × 10(6) pfu/ml, with a recombinant efficiency of 95.8%. In addition, from 6,000 randomly selected and sequenced clones, 5,473 high-quality ESTs were identified. After processing the sequences, 3,961 unigenes representing 897 contigs and 3,064 singlets were obtained with 27.6% redundancy. Analysis of expressed sequenced tags using COG and GO annotation and KEGG pathway data showed that a large group of genes related to growth and development, signal transduction, and defense mechanisms were present in the cDNA library. Based on our findings, this normalized cDNA library will provide a valuable resource for further research on functional genes and ecotoxicology in B. aeruginosa.
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy for Environment Sciences, Beijing 100012, China.
authors.forename: Zi-Cheng
authors.initials: ZC
authors.lastname: Li
authors.forename: Li-Hui
authors.initials: LH
authors.lastname: An
authors.forename: Qing
authors.initials: Q
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Xing-Ru
authors.initials: XR
authors.lastname: Zhao
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Wang
authors.forename: Bing-Hui
authors.initials: BH
authors.lastname: Zheng
authors.forename: Lin-Bo
authors.initials: LB
authors.lastname: Zhang
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003300:Copper / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D020224:Expressed Sequence Tags; D015723:Gene Library; D012313:RNA / Q000302:isolation & purification; D045483:Rivers; D017423:Sequence Analysis, RNA; D012908:Snails / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D014874:Water Pollutants, Chemical; D012313:RNA; D003300:Copper