title: Evaluation of eIF4E expression in an osteosarcoma-specific tissue microarray.
abstract: The ability to define osteosarcoma (OS) patients at greatest risk for metastatic progression and nonresponsiveness to conventional therapy is currently not possible. Such biomarkers are needed to predict overall prognosis, probability of metastases at diagnosis, and response to chemotherapy. The tissue microarray (TMA) serves as a powerful tool for detecting and validating protein biomarkers across a variety of patients. We constructed a novel outcome-linked TMA to add to and address shortcomings of currently available OS tissue resources. To test the use of our TMA, we surveyed the expression of eukaryotic initiation factor 4E (eIF4E) in OS patients using immunohistochemistry. Aberrant regulation of translation initiation is a feature of many cancers. eIF4E is central to initiation of protein synthesis. Its expression and activity have been implicated in tumor formation and potentially malignant and/or metastatic progression in some carcinomas. We found that eIF4E was uniformly expressed in OS patient samples. No association was found between eIF4E and outcome in OS patients. This novel OS TMA provided a facile mechanism to assess the role of a relevant protein biomarker in OS.
authors.affiliation: Tumor and Metastasis Biology Section, Pediatric Oncology Branch, National Institutes of Health, Bethesda, MD 20892, USA. osbornet@mail.nih.gov
authors.forename: Tanasa S
authors.initials: TS
authors.lastname: Osborne
authors.forename: Ling
authors.initials: L
authors.lastname: Ren
authors.forename: John H
authors.initials: JH
authors.lastname: Healey
authors.forename: Lauren Q
authors.initials: LQ
authors.lastname: Shapiro
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Chou
authors.forename: Richard G
authors.initials: RG
authors.lastname: Gorlick
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Hewitt
authors.forename: Chand
authors.initials: C
authors.lastname: Khanna
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D001859:Bone Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D039561:Eukaryotic Initiation Factor-4E / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D012516:Osteosarcoma / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D015996:Survival Rate; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D039561:Eukaryotic Initiation Factor-4E