title: Extraction and determination of opium alkaloids in urine samples using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography.
abstract: A simple, rapid and sensitive method based on dispersive liquid-liquid microextraction (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine opium alkaloids in urine samples. Some effective parameters on extraction were studied and optimized. Under the optimum conditions, enrichment factors and recoveries for different opiates are in the range of 63.0-104.5 and 31.5-52.2%, respectively. The calibration graphs are linear in the range of 0.50-500 μg L(-1) and limit of detections (LODs) are in the range of 0.2-10 μg L(-1). The relative standard deviations (RSDs) for 200 μg L(-1) of morphine, codeine and thebaine, 5.0 μg L(-1) of papaverine and 10.0 μg L(-1) of noscapine in diluted urine sample are in the range of 2.8-6.1% (n=7). The relative recoveries of urine samples spiked with alkaloids are 84.3-106.0%. The obtained results show that DLLME combined with HPLC-UV is a fast and simple method for the determination of opium alkaloids in urine samples.
authors.affiliation: Department Chemistry, Razi University, Kermanshah, Iran. mshamsipur@yahoo.com
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.forename: Nazir
authors.initials: N
authors.lastname: Fattahi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D059627:Liquid Phase Microextraction / Q000379:methods*; D053610:Opiate Alkaloids / Q000302:isolation & purification / Q000652:urine*; D012680:Sensitivity and Specificity
chemical_list: D053610:Opiate Alkaloids