title: Epizootic hemorrhagic disease virus infection of type I interferon receptor deficient mice.
abstract: Type I interferon receptor deficient (IFNAR(-/-)) mice were infected with an Israeli isolate of serotype 7 of epizootic hemorrhagic disease virus (EHDV; Orbivirus, Reoviridae). Two out of two mice that received 5×10(5) 50% tissue culture infectious doses (TCID(50)) by intraperitoneal injection died or were euthanized in a moribund state on day 5 after infection. One mouse out of three that had been inoculated with 5×10(2) TCID(50) died on day 7 while the remaining mice did not show any clinical signs and survived until the end of the experiment. Spleens of all dead mice were highly positive in an EHDV real-time RT-PCR (quantification cycle values ≤15) and contained ≥10(5.8) TCID(50) of virus per ml of homogenate. The viral RNA content and virus titer in the spleens of the two surviving mice, on the other hand, were over 100-fold lower. Different from data reported for BTV, the outcome of EHDV infection of IFNAR(-/-) mice is dose-dependent and subclinical infections can occur.
authors.affiliation: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Insel Riems, Germany. michael.eschbaumer@fli.bund.de
authors.forename: Michael
authors.initials: M
authors.lastname: Eschbaumer
authors.forename: Markus
authors.initials: M
authors.lastname: Keller
authors.forename: Martin
authors.initials: M
authors.lastname: Beer
authors.forename: Bernd
authors.initials: B
authors.lastname: Hoffmann
mesh_terms: D000818:Animals; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D018345:Mice, Knockout; D012367:RNA, Viral / Q000032:analysis; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency / Q000235:genetics; D012088:Reoviridae Infections / Q000235:genetics / Q000821:virology*; D016019:Survival Analysis
chemical_list: D012367:RNA, Viral; D053633:Receptor, Interferon alpha-beta