title: [Effects of astragaloside on IGF-1 and associated protein expression in mice with acute viral myocarditis].
abstract: OBJECTIVE
  To study the effects of astragaloside on the expression of insulin-like growth factor-1 (IGF-1) and associated proteins in mice with viral myocarditis.
  
  
  METHODS
  Sixty-five 4-week-old BALB/C mice were randomly divided into 5 groups: normal control, astragaloside control, untreated myocarditis, low-dose and high-dose astragaloside-treated myocarditis. The BALB/C mice in the later three groups were intraperitoneally injected with CVB3. The low-dose and high-dose astragaloside-treated myocarditis groups were given astragaloside of 0.07 and 0.6 mg/kg•d, respectively by intragastric administration. Fifteen days later, the samples of blood and muscular tissues were obtained. The expression of IGF-1 in plasma was measured using ELISA. The levels of IGF-1 and associated proteins in muscular tissues were measured by immunohistochemistry. The expression of IGF-1 mRNA in muscular tissues was examined by RT-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The expression of IGF-1 and associated proteins increased significantly in mice infected with CVB3. High-dose astragaloside treatment reduced the expression of IGF-1 and associated proteins, but low-dose astragaloside did not.
  
  
  CONCLUSIONS
  High-dose astragaloside may reduce the expression of IGF-1 and associated proteins in mice with acute viral myocarditis, possibly thus providing protective effects on muscular tissues.
authors.affiliation: Department of Pediatrics, Wuhan Pu'ai Hospital, Wuhan, China.
authors.forename: Chun-Zhi
authors.initials: CZ
authors.lastname: He
authors.forename: Shuang-Jie
authors.initials: SJ
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D003384:Coxsackievirus Infections / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D029822:Enterovirus B, Human; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D017526:Receptor, IGF Type 1 / Q000032:analysis; D012503:Saponins / Q000627:therapeutic use*; D014315:Triterpenes / Q000627:therapeutic use*
chemical_list: D004365:Drugs, Chinese Herbal; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1