title: Localization of melatonin receptor 1 in mouse retina and its role in the circadian regulation of the electroretinogram and dopamine levels.
abstract: Melatonin modulates many important functions within the eye by interacting with a family of G-protein-coupled receptors that are negatively coupled with adenylate cyclase. In the mouse, Melatonin Receptors type 1 (MT(1)) mRNAs have been localized to photoreceptors, inner retinal neurons, and ganglion cells, thus suggesting that MT(1) receptors may play an important role in retinal physiology. Indeed, we have recently reported that absence of the MT(1) receptors has a dramatic effect on the regulation of the daily rhythm in visual processing, and on retinal cell viability during aging. We have also shown that removal of MT(1) receptors leads to a small (3-4 mmHg) increase in the level of the intraocular pressure during the night and to a significant loss (25-30%) in the number of cells within the retinal ganglion cell layer during aging. In the present study we investigated the cellular distribution in the C3H/f(+/+) mouse retina of MT(1) receptors using a newly developed MT(1) receptor antibody, and then we determined the role that MT(1) signaling plays in the circadian regulation of the mouse electroretinogram, and in the retinal dopaminergic system. Our data indicate that MT(1) receptor immunoreactivity is present in many retinal cell types, and in particular, on rod and cone photoreceptors and on intrinsically photosensitive ganglion cells (ipRGCs). MT(1) signaling is necessary for the circadian rhythm in the photopic ERG, but not for the circadian rhythm in the retinal dopaminergic system. Finally our data suggest that the circadian regulation of dopamine turnover does not drive the photopic ERG rhythm.
authors.affiliation: Circadian Rhythms and Sleep Disorders Program, Neuroscience Institute and Department of Pharmacology and Toxicology, Morehouse School of Medicine, Atlanta, Georgia, United States of America.
authors.forename: Anamika
authors.initials: A
authors.lastname: Sengupta
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Baba
authors.forename: Francesca
authors.initials: F
authors.lastname: Mazzoni
authors.forename: Nikita V
authors.initials: NV
authors.lastname: Pozdeyev
authors.forename: Enrica
authors.initials: E
authors.lastname: Strettoi
authors.forename: P Michael
authors.initials: PM
authors.lastname: Iuvone
authors.forename: Gianluca
authors.initials: G
authors.lastname: Tosini
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm / Q000235:genetics / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism*; D004596:Electroretinography; D007150:Immunohistochemistry; D051379:Mice; D044122:Receptor, Melatonin, MT1 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*
chemical_list: D044122:Receptor, Melatonin, MT1; D015102:3,4-Dihydroxyphenylacetic Acid; D004298:Dopamine