title: [Application of ICP-MS/ICP-AES to detecting nutrition and heavy metal contents in grain of detached wheat spikes in vitro culture].
abstract: The heavy metals in the environment led to the defect of nutrient contents in grains and metabolic disturbance seriously. In order to research the effect of heavy metal copper and cadmium on nutrient contents and heavy metal contents of wheat grain, the present paper studied the nutrition element (P, K, Ca, Mg, Na, Fe, Mn, Zn and B) and contents of heavy metal (Pb, Cr, Hg, Cu and Cd) in organs of detached wheat spikes in vitro culture by ICP-MS/ICP-AES. The results showed that the weight of grain reduced significantly with copper and cadmium treatment. Under different copper treatment, the contents of P, K, Mg, Ca, Na, Mn and Zn increased, but Fe and B contents reduced. Exogenous cadmium promoted the absorption of P, K, Ca, Mg and Mn while interrupted the absorption of Na, Fe, Zn and B. Exogenous copper and cadmium treatment reduced the Hg content in wheat grain, but the Cu and Cd contents were 55-folds and 62 folds of the national food sanitation standard. All the data showed that the effects of copper and cadmium are disadvantageous to the accumulation of nutrient and heavy metal contents.
authors.affiliation: Key Laboratory of Crop Cultivation and Farming System of the Ministry of Agriculture, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. zdjdai@163.com
authors.forename: Dai-jing
authors.initials: DJ
authors.lastname: Zhang
authors.forename: Li-na
authors.initials: LN
authors.lastname: Jiang
authors.forename: Zhi-juan
authors.initials: ZJ
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Shao
authors.forename: Chun-xi
authors.initials: CX
authors.lastname: Li
mesh_terms: D002104:Cadmium; D003300:Copper; D002523:Edible Grain; D013058:Mass Spectrometry; D019216:Metals, Heavy / Q000032:analysis*; D013054:Spectrophotometry, Atomic; D014908:Triticum / Q000737:chemistry*
chemical_list: D019216:Metals, Heavy; D002104:Cadmium; D003300:Copper