title: Periacinar retraction clefting in nonneoplastic and neoplastic prostatic glands: artifact or molecular involvement.
abstract: A space between neoplastic acini and prostatic stroma is not rare and studies have interpreted this as an artifact, considering the absence of endothelial cells indicating vascular invasion. Thus, the aims of this work were to characterize and correlate the occurrence and extent of retraction clefting with the reactivities of α and β dystroglycan (αDG, βDG), laminin, matrix metalloproteinase 2 (MMP-2), p63, insulin-like growth factor 1(IGF-1), vimentin, and fibroblast growth factor 2 (FGF-2). The study was based on nonneoplastic and neoplastic prostatic tissues obtained from necropsies and retropubic radical prostatectomies. The results showed that periacinar retraction clefting was significantly more frequent in prostatic carcinoma samples than in normal prostatic acini. Most of the neoplastic acini (72.0%) showed retraction clefting of more than 50% of circumference, which were significantly more frequent in Gleason score 7 and 6. Decreased collagen and reticular and elastic fibers were verified in the stroma around neoplastic acini. Weak and discontinuous αDG, βDG, and laminin immunoreactivities and intensified MMP-2, vimentin, IGF-1 and FGF-2 immunoreactivities were verified in the neoplastic acini; p63 immunoreactivity was negative in all carcinomas. Thus, these findings showed that the lack of epithelial basal cells, DGs, and laminin and increased MMP-2, IGF-1, and FGF-7 could be considered important pathways in periacinar retraction occurrence. This study demonstrated the origin of and the biological mechanisms responsible for periacinar retraction clefting in prostatic carcinoma.
authors.affiliation: Department of Anatomy, Institute of Biosciences, Univ Estadual Paulista (UNESP), CP-510, 18618-970, Botucatu, SP, Brazil. wjfavaro@ibb.unesp.br
authors.forename: Wagner José
authors.initials: WJ
authors.lastname: Fávaro
authors.forename: Amanda Cia
authors.initials: AC
authors.lastname: Hetzl
authors.forename: Leonardo Oliveira
authors.initials: LO
authors.lastname: Reis
authors.forename: Ubirajara
authors.initials: U
authors.lastname: Ferreira
authors.forename: Athanase
authors.initials: A
authors.lastname: Billis
authors.forename: Valéria Helena A
authors.initials: VH
authors.lastname: Cagnon
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D000368:Aged; D000369:Aged, 80 and over; D016477:Artifacts; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011467:Prostate / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
chemical_list: D014408:Biomarkers, Tumor