title: Oxidative stress in women with perimenopausal symptoms.
abstract: OBJECTIVE
  In this study, we examined the relationships between perimenopausal symptoms, biochemical parameters, and markers of oxidative stress in women in perimenopause and compared them with those of premenopausal women.
  
  
  METHODS
  Sixty-two women (age, 53.2 ± 5.7 y) with perimenopausal symptoms were recruited to participate in our study. The control group consisted of 18 women without perimenopausal symptoms (age, 40 ± 5 y).Clinical perimenopausal symptoms were evaluated via the questionnaire of the Menopausal Rating Scale. Our participants were checked for basic biochemical parameters. The oxidative status of our samples was determined through the examination of lipoperoxides, 8-oxoguanine (8-oxoG) levels, and the total antioxidant status (TAS).
  
  
  RESULTS
  Perimenopausal women had higher total cholesterol values and lower paraoxonase-1 (PON1) activity compared to reference values. Other biochemical parameters as well as 8-oxoG levels were unchanged compared with those of healthy control women. Lipoperoxide levels were significantly increased compared with those of premenopausal women. We found an indirect correlation between PON1 arylesterase (PON1 A) activity and lipoperoxide levels, between PON1 A activity and atherogenic index, between age and TAS, and between age and 8-oxoG levels. DNA repair ability and the total antioxidant status of women in perimenopause were significantly increased compared with women in premenopause. Hypercholesterolemic women had significantly increased low-density lipoprotein cholesterol levels when compared with normocholesterolemic individuals, but these values were still within the reference range. Normocholesterolemic women had significantly decreased high-density lipoprotein cholesterol levels, below the reference range. We found no correlations between perimenopausal symptoms and biochemical parameters or oxidative stress markers.
  
  
  CONCLUSIONS
  We found that women in perimenopause are under increased oxidative stress manifested by reduced PON1 A activity and elevated lipoperoxidation, DNA repair ability, and TAS. Nutritional antioxidant supplementation may be an effective approach in improving menopausal symptoms.
authors.affiliation: Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovak Republic. ingrid.zitnanova@fmed.uniba.sk
authors.forename: Ingrid
authors.initials: I
authors.lastname: Zitňanová
authors.forename: Martin
authors.initials: M
authors.lastname: Rakovan
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Paduchová
authors.forename: Monika
authors.initials: M
authors.lastname: Dvořáková
authors.forename: Lucia
authors.initials: L
authors.lastname: Andrezálová
authors.forename: Jana
authors.initials: J
authors.lastname: Muchová
authors.forename: Martin
authors.initials: M
authors.lastname: Simko
authors.forename: Iveta
authors.initials: I
authors.lastname: Waczulíková
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Duračková
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004249:DNA Damage; D004260:DNA Repair; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress; D047648:Perimenopause / Q000097:blood*; D017697:Premenopause / Q000097:blood; D011795:Surveys and Questionnaires
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D008054:Lipid Peroxides; C024829:8-hydroxyguanine; D006147:Guanine; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human