title: Surface plasmon resonance biosensor for the detection of VEGFR-1--a protein marker of myelodysplastic syndromes.
abstract: The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
authors.affiliation: Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Pimková
authors.forename: Markéta
authors.initials: M
authors.lastname: Bocková
authors.forename: Kateřina
authors.initials: K
authors.lastname: Hegnerová
authors.forename: Jiří
authors.initials: J
authors.lastname: Suttnar
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Cermák
authors.forename: Jiří
authors.initials: J
authors.lastname: Homola
authors.forename: Jan E
authors.initials: JE
authors.lastname: Dyr
mesh_terms: D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D009190:Myelodysplastic Syndromes / Q000097:blood* / Q000175:diagnosis / Q000378:metabolism; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000097:blood / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1