title: Role of the Arabidopsis leucine aminopeptidase 2.
abstract: Proteolysis-related genes have diverse functions across taxa and have long been considered as key players for intracellular protein turnover. Growing evidence indicates the biological significance of peptidases in degradation, maturation and modulation of bioactive peptides/proteins. By screening T-DNA tagged lines and functional analysis approaches we unraveled the Arabidopsis leucine aminopeptidase (AtLAP2) function in amino acid turnover. Transcriptomics and metabolomics profiling data suggested involvement of AtLAP2 in specific metabolic pathways. Loss-of-function of AtLAP2 resulted in early-leaf senescent and stress-sensitive phenotypes. Our work indicates an important in planta role for AtLAP2 contributing to a further understanding of the proteases having several implications in higher plants.
authors.affiliation: Department of Microbiology, Chulalongkorn University, Bangkok, Thailand. Rungaroon.W@Chula.ac.th
authors.forename: Rungaroon
authors.initials: R
authors.lastname: Waditee-Sirisattha
authors.forename: Akira
authors.initials: A
authors.lastname: Hattori
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
authors.forename: Sophon
authors.initials: S
authors.lastname: Sirisattha
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Takabe
authors.forename: Masafumi
authors.initials: M
authors.lastname: Tsujimoto
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D007931:Leucyl Aminopeptidase / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D013314:Stress, Mechanical; D013379:Substrate Specificity
chemical_list: D029681:Arabidopsis Proteins; D007931:Leucyl Aminopeptidase