title: Simultaneous automatic arteries-veins separation and cerebral blood flow imaging with single-wavelength laser speckle imaging.
abstract: Automatic separation of arteries and veins in optical cerebral cortex images is important in clinical practice and preclinical study. In this paper, a simple but effective automatic artery-vein separation method which utilizes single-wavelength coherent illumination is presented. This method is based on the relative temporal minimum reflectance analysis of laser speckle images. The validation is demonstrated with both theoretic simulations and experimental results applied to the rat cortex. Moreover, this method can be combined with laser speckle contrast analysis so that the artery-vein separation and blood flow imaging can be simultaneously obtained using the same raw laser speckle images data to enable more accurate analysis of changes of cerebral blood flow within different tissue compartments during functional activation, disease dynamic, and neurosurgery, which may broaden the applications of laser speckle imaging in biology and medicine.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics - Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Nengyun
authors.initials: N
authors.lastname: Feng
authors.forename: Jianjun
authors.initials: J
authors.lastname: Qiu
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Sun
authors.forename: Cui
authors.initials: C
authors.lastname: Yin
authors.forename: Weihua
authors.initials: W
authors.lastname: Luo
authors.forename: Shangbin
authors.initials: S
authors.lastname: Chen
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D001158:Arteries / Q000033:anatomy & histology / Q000502:physiology*; D001331:Automation; D001783:Blood Flow Velocity / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007834:Lasers; D051381:Rats; D013997:Time Factors; D014680:Veins / Q000033:anatomy & histology / Q000502:physiology*