title: [Relationship of protease-activated receptor-2 and mast cell in the rat model of small intestinal mucosal damage by short-term administration of non-steroidal anti-inflammatory drug].
abstract: OBJECTIVE
  To identify the expression and significance of protease-activated receptor-2 (PAR-2) and mast cell (MC) in the rat model of small intestinal mucosal damage by a short-term administration of diclofenac.
  
  
  METHODS
  Twenty-four SD-rats were divided into 2 groups (control group and model group, 12 rats each) by random digit table. The rats in the control group were treated with 1 ml distilled water per 250 g, once a day while those in the model group diclofenac 7.5 mg/kg per day. Their terminal ileum was harvested at Day 5 after an intraperitoneal injection. Toluidine blue dyeing was employed to determine the distribution of MC and its count in intestinal mucous membrane. Immunohistochemistry, Western blot and real-time PCR (polymerase chain reaction) were employed analyze the location, expression and change of PAR-2 mRNA in intestinal mucous membrane.
  
  
  RESULTS
  The count of MC was obviously higher in the model group than that in the control group (10.3 ± 2.2 vs 4.2 ± 1.2, P < 0.05). PAR-2 was expressed on the mucous surface, recesses epidermis and lamina propria inflammatory cells. And positive dyes were located within cytoplasm. As compared with the control group, the expression of PAR-2 mRNA was higher (2.63 ± 0.26 vs 1, P < 0.05) and its protein expression higher in the model group (24.3 ± 2.4 vs 17.5 ± 3.5, P < 0.05).
  
  
  CONCLUSIONS
  Both PAR-2 and mast cell are involved in the pathogenesis of small intestinal mucosa injury induced by diclofenac in rats. PAR-2 may be activated by tryptase released from mast cells and participate in the pathogenesis of small intestinal injury as induced by non-steroidal anti-inflammatory drugs.
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310061, China.
authors.forename: Ming-yan
authors.initials: MY
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Lü
authors.forename: Han-qing
authors.initials: HQ
authors.lastname: Chen
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects*; D004008:Diclofenac / Q000009:adverse effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044464:Receptor, PAR-2 / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D044464:Receptor, PAR-2; D004008:Diclofenac