title: Elevation of plasma soluble CD26 levels during pregnancy.
abstract: OBJECTIVE
  CD26 is a type II transmembrane protein with dipeptidyl peptidase IV (DPPIV) activity expressed on a variety of cell types. Recent studies have indicated that CD26 or enzymatic activity levels were previously associated with immune-mediated disorders. As immunoregulation is very important for a successful pregnancy, we hypothesize that CD26 may play an important role during pregnancy, and herein, we sought to determine the association between circulating levels of soluble CD26 (sCD26) and pregnancy outcome.
  
  
  METHODS
  In this study, a stable hybridoma cell line 1F1 was produced by fusion of murine splenocytes and myeloma cells. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed for the detection of the maternal plasma sCD26. We measured the plasma levels of sCD26 in 80 normal pregnant women and 45 non-pregnant women.
  
  
  RESULTS
  Our results indicated that the plasma level of sCD26 was significantly higher in the pregnant group (P < 0.001) than that in the non-pregnant group.
  
  
  CONCLUSIONS
  These findings hinted that CD26 may play a role in successful pregnancy and it is not an absolute surrogate marker for the Th1-type immunity as the dominant Th2-type immunity during pregnancy.
authors.affiliation: Departments of Blood Transfusion Obstetrics and Gynecology, Southwest Hospital Department of Clinical Biochemistry, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing, China. chuminhu@163.com
authors.forename: Shuming
authors.initials: S
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Song
authors.forename: Yahan
authors.initials: Y
authors.lastname: Fan
authors.forename: Qing
authors.initials: Q
authors.lastname: Chang
authors.forename: Weijing
authors.initials: W
authors.lastname: Yi
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Chuanmin
authors.initials: C
authors.lastname: Hu
mesh_terms: D000328:Adult; D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D051379:Mice; D011247:Pregnancy / Q000097:blood*
chemical_list: D018819:Dipeptidyl Peptidase 4