title: Pathogenic autoreactive B cells are not negatively selected toward matrix protein collagen II.
abstract: We have addressed the importance of B cell tolerance to collagen type II, a matrix protein, which is a target in rheumatoid arthritis (RA) and its mouse models. We generated a germline-encoded anti-collagen type II (CII) IgH replacement anti-C1 B cell mouse strain (ACB) to investigate how B cell tolerance to CII, a matrix protein, is subverted and to further understand pathogenesis of RA. Phenotypic analysis revealed that CII-specific B cells were surprisingly neither deleted nor anergized. Instead, they were readily detected in all lymphoid organs. Spontaneously produced autoantibodies could bind directly to cartilage surface without detectable pathology. However, exaggerated arthritis was seen after injection of anti-CII Abs specific for other epitopes. In addition, Abs from CII-specific hybridomas generated from ACB mice induced arthritis. Interestingly, IgH/L chain sequence data in B cell hybridomas revealed a lack of somatic mutations in autoreactive B cells. The ACB model provides the first possibility, to our knowledge, to study B cell tolerance to a matrix protein, and the observations made in the study could not be predicted from previous models. B cell-reactive epitopes on CII are largely shared between human RA and rodent CII-induced arthritis; this study, therefore, has important implications for further understanding of pathological processes in autoimmune diseases like RA.
authors.affiliation: Medical Inflammation Research, Lund University, Lund, Sweden.
authors.forename: Duojia
authors.initials: D
authors.lastname: Cao
authors.forename: Ia
authors.initials: Ia
authors.lastname: Khmaladze
authors.forename: Hongwei
authors.initials: H
authors.lastname: Jia
authors.forename: Estelle
authors.initials: E
authors.lastname: Bajtner
authors.forename: Kutty S
authors.initials: KS
authors.lastname: Nandakumar
authors.forename: Thomas
authors.initials: T
authors.lastname: Blom
authors.forename: John A
authors.initials: JA
authors.lastname: Mo
authors.forename: Rikard
authors.initials: R
authors.lastname: Holmdahl
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001323:Autoantibodies / Q000378:metabolism; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D001666:Binding Sites, Antibody; D024043:Collagen Type II / Q000276:immunology*; D004195:Disease Models, Animal; D018985:Epitopes, B-Lymphocyte / Q000276:immunology; D016326:Extracellular Matrix Proteins / Q000276:immunology* / Q000378:metabolism; D055879:Gene Knock-In Techniques; D007108:Immune Tolerance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D001323:Autoantibodies; D024043:Collagen Type II; D018985:Epitopes, B-Lymphocyte; D016326:Extracellular Matrix Proteins