title: Autoclaving as a chemical-free process to stabilize recombinant silk-elastinlike protein polymer nanofibers.
abstract: We report here that autoclaving is a chemical-free, physical crosslinking strategy capable of stabilizing electrospun recombinant silk-elastinlike protein (SELP) polymer nanofibers. Fourier transform infrared spectroscopy showed that the autoclaving of SELP nanofibers induced a conformational conversion of β-turns and unordered structures to ordered β-sheets. Tensile stress-strain analysis of the autoclaved SELP nanofibrous scaffolds in phosphate buffered saline at 37 °C revealed a Young's modulus of 1.02 ± 0.28 MPa, an ultimate tensile strength of 0.34 ± 0.04 MPa, and a strain at failure of 29% ± 3%.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Qiu
authors.forename: Joseph
authors.initials: J
authors.lastname: Cappello
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wu