title: Response surface optimization of the heparosan N-deacetylation in producing bioengineered heparin.
abstract: The chemical step in the chemoenzymatic synthesis of bioengineered heparin has been examined and optimized statistically using a response surface methodology. A four factor, two level full factorial design experiment and a three factor Box-Behnken design were carried out. The goal was to establish a method to prepare N-sulfo, N-acetyl heparosan of the desired N-acetyl content, number average molecular weight, and in maximum yield by controlling the reactant concentrations, reaction time and reaction temperature. The response surface models obtained were used to predict the reaction conditions required to optimally prepare N-sulfo, N-acetyl heparosan from Escherichia coli generated heparosan starting material of different molecular weights.
authors.affiliation: Department of Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Jennifer
authors.initials: J
authors.lastname: Li
authors.forename: Samantha
authors.initials: S
authors.lastname: Cheong
authors.forename: Ujjwal
authors.initials: U
authors.lastname: Bhaskar
authors.forename: Onishi
authors.initials: O
authors.lastname: Akihiro
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Dordick
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000107:Acetylation; D001698:Biomedical Engineering; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006493:Heparin / Q000096:biosynthesis* / Q000737:chemistry; D008970:Molecular Weight; D013696:Temperature
chemical_list: D004187:Disaccharides; C560363:heparosan; D006493:Heparin