title: Neural differentiation of induced pluripotent stem cells.
abstract: The great potential of induced pluripotent cells (iPS) cells is that it allows the possibility of deriving pluripotent stem cells from any human patient. Generation of patient-derived stem cells serves as a great source for developing cell replacement therapies and also for creating human cellular model systems of specific diseases or disorders. This is only of benefit if there are well-established differentiation assay systems to generate the cell types of interest. This chapter describes robust and well-characterized protocols for differentiating iPS cells to neural progenitors, neurons, glia and neural crest cells. These established assays can be applied to iPS cell lines derived from patients with neurodegenerative disorders to study cellular mechanisms associated with neurodegeneration as well as investigating the regenerative potential of patient derived stem cells.
authors.affiliation: Centre for Neuroscience, Department of Pharmacology, University of Melbourne, Parkville, VIC, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Denham
authors.forename: Mirella
authors.initials: M
authors.lastname: Dottori
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002352:Carrier Proteins / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D018920:Coculture Techniques; D003584:Cytological Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D019636:Neurodegenerative Diseases / Q000473:pathology; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000099176:Noggin Protein
chemical_list: D015415:Biomarkers; D002352:Carrier Proteins; D016685:Mitomycin; D000099176:Noggin Protein