title: Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses.
abstract: The selection of suitable reference genes is crucial for accurate quantification of gene expression. To identify suitable reference genes in Beauveria bassiana, the expression of 14 candidates (18S, 28S, β-Tub, GAPD, γ-Act, TEF, HGPT, His3, His2A, TBP, CypA, CypB, PP1, and CrzA) was measured by quantitative polymerase chain reaction at different development stages and under various nutritional and stress conditions. Expression stability, as evaluated by the geNorm and NormFinder programs, revealed that His2A/γ-Act/CrzA was the most stably expressed set of genes throughout development, while 28S/PP1/CypA and His2A/γ-Act/CypA were the most stably expressed gene sets under a variety of nutritional and stress conditions, respectively. Overall, the most stably expressed genes under all conditions examined were PP1, γ-Act, and CypA.
authors.affiliation: Biotechnology Research Center, Southwest University, 215 Tiansheng Road, Beibei, 400716, Chongqing, People's Republic of China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhou
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Zhi-Bing
authors.initials: ZB
authors.lastname: Luo
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Fan
authors.forename: Gui-Rong
authors.initials: GR
authors.lastname: Tang
authors.forename: Li-Ji
authors.initials: LJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Pei
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D052982:Beauveria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D004271:DNA, Fungal