title: Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions.
abstract: An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.
authors.affiliation: Harold C, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA. amyn.habib@utsouthwestern.edu.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Dong
authors.forename: Ameena J
authors.initials: AJ
authors.lastname: Jiwani
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Zou
authors.forename: Johanne
authors.initials: J
authors.lastname: Pastor
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuro-O
authors.forename: Amyn A
authors.initials: AA
authors.lastname: Habib
authors.forename: Minzi
authors.initials: M
authors.lastname: Ruan
authors.forename: David A
authors.initials: DA
authors.lastname: Boothman
authors.forename: Chin-Rang
authors.initials: CR
authors.lastname: Yang