title: 5-Hydroxymethylcytosine-selective oxidation with peroxotungstate.
abstract: Tungsten oxidation worked as a simple chemical reaction for the effective detection of 5-hydroxymethylcytosine in DNA, distinguishing it from its epigenetic precursors, 5-methylcytosine and unmethylated cytosine. The tungsten-oxidation product obtained from 5-hydroxymethylcytosine was trihydroxylated thymine and was detected as a cleavage band in gel electrophoresis after treatment with hot piperidine.
authors.affiliation: Advanced Science Institute, RIKEN, Wako, Saitama 351-0198, Japan. aki-okamoto@riken.jp
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Shuji
authors.initials: S
authors.lastname: Ikeda
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000235:genetics; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D017973:Tungsten Compounds / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D017973:Tungsten Compounds; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; C045951:tungstate