title: Measurement of phosphorothioate oligodeoxynucleotide antisense transport across the blood-brain barrier.
abstract: Phosphorothioate oligodeoxynucleotides (PODNs) can act as antisense molecules, knocking down proteins. Many PODNs have the unusual characteristic of being transported across the blood-brain barrier by a saturable system. This means that PODNs injected intravenously can accumulate in the central nervous system in quantities sufficient to knock down proteins in brain and the blood-brain barrier. A critical step in the development of PODNs that can be administered peripherally and knockdown proteins in the central nervous system is to determine the relation to the blood-brain barrier, specifically, does the PODN cross the blood-brain barrier and, if so, how fast and to what degree.
authors.affiliation: Geriatrics Research, Education, and Clinical Center, Puget Sound Health Care System, Seattle, WA, USA. wabanks1@u.washington.edu
authors.forename: William A
authors.initials: WA
authors.lastname: Banks
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D054735:Phosphorothioate Oligonucleotides / Q000378:metabolism
chemical_list: D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D054735:Phosphorothioate Oligonucleotides