title: Skeletal muscle signaling response to sprint exercise in men and women.
abstract: To determine if there is a sex dimorphism in the skeletal muscle signaling response to sprint exercise, 17 men and ten women performed a 30-s Wingate test. Muscle biopsies were taken before, immediately after the exercise and at 30 and 120 min during the recovery period. Thr(172)-AMPKα, Ser(221)-ACCβ, Thy(705)-STAT3, Thr(202)/Thy(204)-ERK1/2 and Thr(180)/Thy(182)-p38MAPK phosphorylation responses to sprint exercise were not statistically different between men and women. AMPKα phosphorylation was enhanced fourfold 30 min after the sprint exercise in males and females (P < 0.01). ACCβ phosphorylation was enhanced by about threefold just after the sprint test exercise and 30 min into the recovery period in males and females (P < 0.01). STAT3 phosphorylation was increased 2 h after the Wingate test compared to the value observed right after the end of the exercise (P < 0.05), and 30 min after the Wingate test there was a 2.5-fold increase in ERK1/2 phosphorylation, compared to both the pre-exercise and to the value observed right after the Wingate test (both, P < 0.05). In conclusion, the skeletal muscle signaling response to a single bout of sprint exercise mediated by AMPK, ACC, STAT3, ERK and p38MAPK is not statistically different between men and women. Marked increases in AMPKα, ACCβ, STAT3 and ERK phosphorylation were observed after a single 30-s all-out sprint (Wingate test) in the vastus lateralis.
authors.affiliation: Department of Physical Education, Campus Universitario de Tafira, University of Las Palmas de Gran Canaria, 35017 Las Palmas de Gran Canaria, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Fuentes
authors.forename: Borja
authors.initials: B
authors.lastname: Guerra
authors.forename: Jesús G
authors.initials: JG
authors.lastname: Ponce-González
authors.forename: David
authors.initials: D
authors.lastname: Morales-Alamo
authors.forename: Amelia
authors.initials: A
authors.lastname: Guadalupe-Grau
authors.forename: Hugo
authors.initials: H
authors.lastname: Olmedillas
authors.forename: Lorena
authors.initials: L
authors.lastname: Rodríguez-García
authors.forename: David
authors.initials: D
authors.lastname: Feijoo
authors.forename: Pedro
authors.initials: P
authors.lastname: De Pablos-Velasco
authors.forename: Leandro
authors.initials: L
authors.lastname: Fernández-Pérez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Santana
authors.forename: Jose A L
authors.initials: JA
authors.lastname: Calbet
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000328:Adult; D005080:Exercise Test; D005260:Female; D006801:Humans; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D012420:Running / Q000502:physiology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D012727:Sex Characteristics; D015398:Signal Transduction; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D050796:STAT3 Transcription Factor; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase