title: Nanopore sensors for nucleic acid analysis.
abstract: Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.
authors.affiliation: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61820, USA.
authors.forename: Bala Murali
authors.initials: BM
authors.lastname: Venkatesan
authors.forename: Rashid
authors.initials: R
authors.lastname: Bashir
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000639:trends; D004277:DNA, Single-Stranded / Q000032:analysis*; D015894:Genome, Human; D006801:Humans; D058608:Nanopores; D012313:RNA / Q000032:analysis*; D017422:Sequence Analysis, DNA / Q000295:instrumentation / Q000379:methods* / Q000639:trends
chemical_list: D004277:DNA, Single-Stranded; D012313:RNA