title: An electrospun degradable scaffold based on a novel hydrophilic polyester for tissue-engineering applications.
abstract: Scaffolds based on a novel functionalized polyester, pHMGCL, are electrospun and characterized morphologically and physically. In vitro degradation studies of pHMGCL films show considerable mass loss and molecular weight reduction within 70 weeks. Scaffolds composed of fibers with uniform diameter (≈ 900 nm) and with melting temperatures higher than body temperature are prepared. As an indication for the feasibility of this material for regenerative medicine approaches, articular chondrocytes are seeded onto electrospun pHMGCL scaffolds. Chondrocytes attach to the fibers and re-differentiate as demonstrated by the production of GAG and collagen type II within four weeks of in vitro culture. Hydrophilic pHMGCL scaffolds may thus be useful for tissue engineering applications.
authors.affiliation: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, P O Box 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Hajar
authors.initials: H
authors.lastname: Seyednejad
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Wouter
authors.initials: W
authors.lastname: Schuurman
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
authors.forename: Jos
authors.initials: J
authors.lastname: Malda
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Cornelus
authors.initials: C
authors.lastname: van Nostrum
authors.forename: Tina
authors.initials: T
authors.lastname: Vermonden
authors.forename: Wim E
authors.initials: WE
authors.lastname: Hennink
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D001673:Biodegradation, Environmental; D002358:Cartilage, Articular / Q000166:cytology* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D002850:Chromatography, Gel; D024043:Collagen Type II / Q000096:biosynthesis; D006736:Horses; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D011091:Polyesters; C016240:polycaprolactone