title: Electrochemical oxidation of glucose using mutant glucose oxidase from directed protein evolution for biosensor and biofuel cell applications.
abstract: In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.
authors.affiliation: School of Chemical Engineering and Advanced Materials, Merz Court, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK. eileen.yu@ncl.ac.uk
authors.forename: Eileen Hao
authors.initials: EH
authors.lastname: Yu
authors.forename: Radivoje
authors.initials: R
authors.lastname: Prodanovic
authors.forename: Güray
authors.initials: G
authors.lastname: Güven
authors.forename: R
authors.initials: R
authors.lastname: Ostafe
authors.forename: U
authors.initials: U
authors.lastname: Schwaneberg
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000379:methods*; D019020:Directed Molecular Evolution; D055664:Electrochemical Techniques / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D010084:Oxidation-Reduction
chemical_list: D005656:Fungal Proteins; D005949:Glucose Oxidase; D005947:Glucose