title: CAGE (cap analysis of gene expression): a protocol for the detection of promoter and transcriptional networks.
abstract: We provide here a protocol for the preparation of cap-analysis gene expression (CAGE) libraries, which allows for measuring the expression of eukaryotic capped RNAs and simultaneously map the promoter regions. The presented protocol simplifies the previously published ones and moreover produces tags that are 27 nucleotides long, which facilitates mapping to the genome. The protocol takes less than 5 days to complete and presents a notable improvement compared to previously published versions.
authors.affiliation: Riken Omics Science Center, Riken Yokohama Institute, Yokohama, Kanagawa, Japan.
authors.forename: Hazuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Sachi
authors.initials: S
authors.lastname: Kato
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Murata
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000235:genetics; D015723:Gene Library; D053263:Gene Regulatory Networks / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012315:RNA Caps / Q000032:analysis* / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D012315:RNA Caps