title: Endocrine effects of methoxylated brominated diphenyl ethers in three in vitro models.
abstract: Methoxylated brominated diphenyl ethers (MeO-BDEs) in aquatic environments have been found to be primarily of natural origin in the marine environment and not from biotransformation of synthetic PBDEs. Two of the eight MeO-PBDEs (2'-MeO-BDE-68 and 6-MeO-BDE-47) that were detected in anchovy from the Yangtze River Delta, were natural products from marine organisms. So 2'-MeO-BDE-68 and 6-MeO-BDE-47 were chosen to study the potential to modulate androgen, estrogen, or thyroid hormone receptor- (AR, ER, ThR) mediated responses by use of reporter gene assays. 2'-MeO-BDE-68 was antiandrogenic at 50 μM, estrogenic at 10 μM and antiestrogenic at 10 and 50 μM (IC50=4.88 μM). 2'-MeO-BDE-68 enhanced luciferase expression by 5 nM T3 at 50 μM. 6-MeO-BDE-47 exhibited potent antiandrogenicity at 1 μM and greater (IC50=41.8 μM) and possessed estrogenic activity at 10 μM and antiestrogenic activity at 10 and 50 μM (IC50=6.02 μM).
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Hongling
authors.initials: H
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.forename: Ouxi
authors.initials: O
authors.lastname: Shen
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
authors.forename: Michael H W
authors.initials: MH
authors.lastname: Lam
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
mesh_terms: D000704:Analysis of Variance; D059002:Androgen Receptor Antagonists / Q000633:toxicity*; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D017930:Genes, Reporter / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D008156:Luciferases / Q000378:metabolism*; D051379:Mice; D015394:Molecular Structure; D010957:Plasmids / Q000235:genetics; D051381:Rats; D011960:Receptors, Estrogen / Q000819:agonists* / Q000037:antagonists & inhibitors*; D011988:Receptors, Thyroid Hormone / Q000819:agonists / Q000037:antagonists & inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D059002:Androgen Receptor Antagonists; D052244:Endocrine Disruptors; D055768:Halogenated Diphenyl Ethers; D011960:Receptors, Estrogen; D011988:Receptors, Thyroid Hormone; D013778:Tetrazolium Salts; D013844:Thiazoles; D008156:Luciferases; C022616:thiazolyl blue