title: Characterization of plasmid pPO1 from the hyperacidophile Picrophilus oshimae.
abstract: Picrophilus oshimae and Picrophilus torridus are free-living, moderately thermophilic and acidophilic organisms from the lineage of Euryarchaeota. With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known. So far, nothing is known about plasmid biology in these hyperacidophiles. Also, there are no genetic tools available for this genus. We have mobilized the 7.6 Kbp plasmid from P. oshimae in E. coli by introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy number in the native host, mode of replication, and transcriptional start sites of the encoded ORFs. Plasmid pPO1 may encode a restriction/modification system in addition to its replication functions. The information gained from the pPO1 plasmid may prove useful in developing a cloning system for this group of extreme acidophiles.
authors.affiliation: Lehrstuhl für Mikrobiologie, Technische Universität München, Freising, Germany.
authors.forename: Angel
authors.initials: A
authors.lastname: Angelov
authors.forename: Jörn
authors.initials: J
authors.lastname: Voss
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Liebl
mesh_terms: D004261:DNA Replication; D004251:DNA Transposable Elements; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli; D019605:Euryarchaeota; D022761:Gene Transfer, Horizontal; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D013464:Sulfuric Acids / Q000378:metabolism; D019603:Thermoplasmales / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D004251:DNA Transposable Elements; D019641:DNA, Archaeal; D013464:Sulfuric Acids; C033158:sulfuric acid