title: A novel BMT technique for treatment of various currently intractable diseases.
abstract: A recently-developed BMT method combines a "Perfusion Method" (PM) for collecting bone marrow cells (BMCs) with the Intra-Bone Marrow (IBM) injection of BMCs (IBM-BMT). As distinct from the conventional aspiration method (AM), the PM allows rapid (within 1 h) collection of BMCs without T cell contamination (T cells < 10%). Therefore, no GvHD occurs. Moreover, the burden on donors, such as back pain, bleeding and infection, can be reduced. Full chimerism can be achieved even with only mild conditioning regimens if IBM-BMT is carried out, since IBM-BMT replaces not only the recipient's hemopoietic stem cells (HSCs) but also mesenchymal stem cells (MSCs) with donor-derived HSCs and MSCs. Using this method, we show that most currently intractable diseases are HSC or MSC disorders, and that this novel strategy (PM + IBM-BMT) can be used to treat various otherwise intractable diseases (including autoimmune diseases and age-associated diseases). We believe that the development of this technique will herald a revolution in the field of BMT, regeneration medicine and also organ transplantation.
authors.affiliation: Department of Stem Cell Disorders, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi City, Osaka, Japan. ikehara@takii.kmu.ac.jp
authors.forename: Susumu
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000379:methods*; D017074:Common Variable Immunodeficiency / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology / Q000517:prevention & control; D006801:Humans; D008252:Macaca fascicularis; D051379:Mice; D008809:Mice, Inbred C3H; D018183:Transplantation Chimera / Q000276:immunology; D014184:Transplantation, Homologous