title: Transcriptome profiling of degU expression reveals unexpected regulatory patterns in Bacillus megaterium and discloses new targets for optimizing expression.
abstract: The first whole transcriptome assessment of a Bacillus megaterium strain provides unanticipated insights into the degSU regulon considered to be of central importance for exo-enzyme production. Regulatory patterns as well as the transcription of degSU itself deviate from the model organism Bacillus subtilis; the number of DegU-regulated secretory enzymes is rather small. Targets for productivity optimization, besides degSU itself, arise from the unexpected DegU-dependent induction of the transition-state regulator AbrB during exponential growth. Induction of secretion-assisting factors, such as the translocase subunit SecY or the signal peptidase SipM, promote hypersecretion. B. megaterium DegSU transcriptional control is advantageous for production purposes, since the degU32 constitutively active mutant conferred hypersecretion of a heterologous Bacillus amyloliquefaciens amylase without the detrimental rise, as for B. subtilis and Bacillus licheniformis, in extracellular proteolytic activities.
authors.affiliation: Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms Universität, Corrensstrasse 3, 48149, Münster, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Borgmeier
authors.forename: Rebekka
authors.initials: R
authors.lastname: Biedendieck
authors.forename: Kristina
authors.initials: K
authors.lastname: Hoffmann
authors.forename: Dieter
authors.initials: D
authors.lastname: Jahn
authors.forename: Friedhelm
authors.initials: F
authors.lastname: Meinhardt
mesh_terms: D001410:Bacillus megaterium / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C066497:DegU protein, Bacteria