title: [Real-time PCR assay for detection and quantification of human adenoviruses in patients with haematological malignancies and symptoms of lower respiratory tract infection].
abstract: Human adenoviruses (HAdV) are one of the im-portant infectious etiological factors that affect immunocompromised patients. Because of the large number of HAdV serotypes and their genomic variations, they present a lot of difficulty in laboratory diagnostics. The recent introduction of real-time PCR (qPCR)-based assays has opened new ways to rapid, specific, and highly sensitive HAdV detection. For detection and quantification of HAdV DNA we retrospectively tested serum and bronchoalveolar lavage fluid (BALF) samples obtained from a cohort of 60 adult patients with haematological malignancies presenting clinical and radiological symptoms of lower respiratory tract infections. Human adenoviruses DNA was detected by qPCR method, using primers targeting a conserved region of the adenoviral hexon gene and a specific TaqMan probe. Adenovirus infection occurred with a high incidence in our study group patients. Using qPCR we found that a 21,7% and 15,0% of patients had adenoviral DNA in BALF and serum samples, respectively. The high level of sensitivity, specificity and accuracy provided by real-time PCR assay are favorable for the use in the detection of adenoviral DNA in clinical specimens, especially in immunocompromised patients.
authors.affiliation: Katedra i Zakład Mikrobiologii Lekarskiej, Warszawski Uniwersytet Medyczny.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Rynans
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dzieciatkowski
authors.forename: Rafał
authors.initials: R
authors.lastname: Krenke
authors.forename: Magdalena
authors.initials: M
authors.lastname: Grabczak
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kołkowska-Leśniak
authors.forename: Maciej
authors.initials: M
authors.lastname: Przybylski
authors.forename: Agata
authors.initials: A
authors.lastname: Sulowsk
authors.forename: Ryszarda
authors.initials: R
authors.lastname: Chazan
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Warzocha
authors.forename: Grazyna
authors.initials: G
authors.lastname: Młynarczyk
mesh_terms: D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000328:Adult; D036022:Capsid Proteins / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D055815:Young Adult
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus