title: Database of published retention factors for immobilized artificial membrane HPLC and an assessment of the effect of experimental variability.
abstract: A database was collated of published experimental logarithmic values for the relative retention factors (log k(IAM)) measured using an immobilized artificial membrane column and high-performance liquid chromatography (IAM HPLC). Log k(IAM) is an alternative measure of hydrophobicity to the octanol/water partition coefficient (log K(OW)). While there are several accepted methods to measure log K(OW), no standardized method exists to determine log k(IAM). The database of collated log k(IAM) values includes 13 key experimental parameters and contains 1,686 values for 555 compounds, which are predominantly polar organic compounds and include drug molecules and surfactants. These compounds are acidic, basic, and neutral and both ionized and un-ionized under the conditions of analysis. The data compiled demonstrated experimental variability for each experimental parameter considered, including column stationary phase, pH, temperature, and mobile phase. Reducing the experimental variability allowed for greater consistency in the datasets.
authors.affiliation: School of Pharmacy and Chemistry, Liverpool John Moores University, Liverpool, UK.
authors.forename: M R
authors.initials: MR
authors.lastname: Ledbetter
authors.forename: S
authors.initials: S
authors.lastname: Gutsell
authors.forename: G
authors.initials: G
authors.lastname: Hodges
authors.forename: J C
authors.initials: JC
authors.lastname: Madden
authors.forename: S
authors.initials: S
authors.lastname: O'Connor
authors.forename: M T D
authors.initials: MT
authors.lastname: Cronin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016208:Databases, Factual; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D000442:Octanols / Q000737:chemistry; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000737:chemistry*; D014865:Waste Disposal, Fluid; D014867:Water / Q000737:chemistry
chemical_list: D004785:Environmental Pollutants; D008567:Membranes, Artificial; D000442:Octanols; D004364:Pharmaceutical Preparations; D014867:Water