title: Overexpression of members of the microRNA-183 family is a risk factor for lung cancer: a case control study.
abstract: BACKGROUND
  Lung cancer is the leading cause of cancer-related deaths worldwide. Early detection is considered critical for lung cancer treatment. MicroRNAs (miRNAs) have shown promise as diagnostic and prognostic indicators. This study was to identify specific miRNAs with diagnostic and prognostic value for patients with lung cancer, and to explore the correlation between expression profiles of miRNAs and patient survival.
  
  
  METHODS
  Gene expression of members of the miR-183 family (miR-96, miR-182, and miR-183) were examined in 70 paired samples from lung cancer patients (primary cancer and non-cancerous tissues and sera), as well as 44 serum samples from normal volunteers and lung cancer cell lines by quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR). The correlation between the expression of miRNAs in tissues, sera, and patient overall survival were also examined by log-rank and Cox regression analysis.
  
  
  RESULTS
  Expression levels of members of the miR-183 family in lung cancer tumor and sera were higher than that of their normal counterparts. The miR-96 expression in tumors was positively associated with its expression in sera. Log-rank and Cox regression analyses demonstrated that high expression of tumor and serum miRNAs of the miR-183 family were associated with overall poor survival in patients with lung cancer.
  
  
  CONCLUSIONS
  Our results suggest that the expressions of miR-96, miR-182, and miR-183 in tumor and sera may be considered potential novel biomarkers for the diagnosis and prognosis of lung cancer.
authors.affiliation: Department of Cardio-Thoracic Surgery, Zhoushan Hospital of Zhejiang Province, Zhoushan, Zhejiang, 316004, 316004, China.
authors.forename: Wangyu
authors.initials: W
authors.lastname: Zhu
authors.forename: XiaoGuang
authors.initials: X
authors.lastname: Liu
authors.forename: JianYing
authors.initials: J
authors.lastname: He
authors.forename: DongDong
authors.initials: D
authors.lastname: Chen
authors.forename: YanYan
authors.initials: Y
authors.lastname: Hunag
authors.forename: Yong Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D015203:Reproducibility of Results; D012307:Risk Factors; D018709:Statistics, Nonparametric
chemical_list: D014408:Biomarkers, Tumor; C533449:MIRN183 microRNA, human; D035683:MicroRNAs