title: Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan.
abstract: Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In the second part of the study, we developed a duplex PCR assay for the detection of AAV and CAV which is capable to simultaneously detect both the viral types prevalent in Pakistan with high sensitivity and 100% specificity.
authors.affiliation: Institute of Biotechnology and Genetic Engineering, KPK Agricultural University, Peshawar 25000, Pakistan.
authors.forename: Latif U
authors.initials: LU
authors.lastname: Rehman
authors.forename: Bakht
authors.initials: B
authors.lastname: Sultan
authors.forename: Ijaz
authors.initials: I
authors.lastname: Ali
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Bhatti
authors.forename: Sana U
authors.initials: SU
authors.lastname: Khan
authors.forename: Khaliq U
authors.initials: KU
authors.lastname: Zaman
authors.forename: Anila T
authors.initials: AT
authors.lastname: Jahangiri
authors.forename: Najib U
authors.initials: NU
authors.lastname: Khan
authors.forename: Aqib
authors.initials: A
authors.lastname: Iqbal
authors.forename: Jehan
authors.initials: J
authors.lastname: Bakht
authors.forename: Zahoor A
authors.initials: ZA
authors.lastname: Swati
authors.forename: Muti U
authors.initials: MU
authors.lastname: Rehman
mesh_terms: D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics*; D001853:Bone Marrow / Q000737:chemistry; D017727:Chicken anemia virus / Q000235:genetics*; D002645:Chickens / Q000821:virology*; D018173:Circoviridae Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D060085:Coinfection; D004279:DNA, Viral / Q000032:analysis*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010154:Pakistan; D011201:Poultry Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D015995:Prevalence; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000737:chemistry
chemical_list: D004279:DNA, Viral