title: Tungstate: is it really a specific nitrate reductase inhibitor in plant nitric oxide research?
abstract: Nitrate reductase (NR) is an enzymatic source of nitric oxide (NO) in plants, and it needs Mo for the Mo-cofactor to be activated. Because NR-deficient mutants are not always available in some species, a cheap and simple pharmacological application of tungstate, which substitutes for Mo in the Mo-cofactor as a competitive antagonist, is widely used as a NR inhibitor in plant NO research. However, evidence indicates that tungstate not only inactivates NR but also inhibits other molybdate-dependent enzymes in plants. In addition, a number of investigations have shown that tungstate also inhibits root growth, affects cortical microtubule formation, and induces programmed cell death (PCD) in plants, just like other heavy metals do. Therefore, tungstate has been shown to exert many other effects that are not connected with the inhibition of NR activity. The origin and mechanism of using tungstate as a NR inhibitor in plants is reviewed here and the progress regarding tungstate toxicity to plants and the possible problems involved in using tungstate as a NR inhibitor in plant NO research are analysed. In summary, the use of tungstate as a NR inhibitor in plant NO research must be treated with caution, keeping in mind that it is not completely specific. It is necessary to search for more NR-deficient mutants and new, specific NR inhibitors. A combination of pharmacological and biochemical analysis with a genetic approach will be necessary in order to investigate the roles of NO in plants.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou 310006, People's Republic of China. jiexiong@zju.edu.cn
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.forename: Guanfu
authors.initials: G
authors.lastname: Fu
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhu
authors.forename: Longxing
authors.initials: L
authors.lastname: Tao
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology*; D050901:Nitrate Reductase / Q000037:antagonists & inhibitors*; D009569:Nitric Oxide / Q000378:metabolism*; D012106:Research; D017973:Tungsten Compounds / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D017973:Tungsten Compounds; D009569:Nitric Oxide; D050901:Nitrate Reductase; C045951:tungstate