title: Assessing the effects of polychlorinated biphenyls (Aroclor 1254) on a scleractinian coral (Stylophora pistillata) at organism, physiological, and molecular levels.
abstract: Polychlorinated biphenyls (PCBs) are a group of widespread contaminants, and accumulation of PCBs has been observed in corals in the field. However, the toxic effects of PCBs on corals have not been investigated. In this study, we tested short and long term toxicity of Aroclor 1254, a commercial PCB mixture, on the scleractinian coral Stylophora pistillata. Coral nubbins were incubated in either control seawater or seawater dosed with PCBs (approximately 300ng/L) for 96h. The effect of PCB exposure on coral gene expression at 4h post exposure was tested with the suppression subtractive hybridization (SSH) and quantitative PCR methods. Photosystem II activity of the zooxanthellae was measured at 96h. After the exposure, nubbins were moved into clean seawater and their survival and growth were observed for another 50 days. All nubbins survived during the exposure and the following 50-d recovery period. Photosystem II activity and coral growth were not affected by PCB exposure in this study. Fifty-four clones were sequenced for gene expression analysis, and 15% of these sequences were identified, including genes involved in general stress response, peptide metabolism, cellular receptor, cytoskeleton organization, membrane trafficking, and oxidative stress response. However, the quantitative PCR did not show significant difference in the five selected genes. In conclusion, acute exposure of S. pistillata to Aroclor 1254 at 300ng/L did not affect coral survival, photosynthesis or growth but may alter the expression of certain genes involved in various important cellular functions. The nubbin technique proved to be an efficient approach to simultaneously characterize the impact of PCBs on the corals at multiple biological levels.
authors.affiliation: National Museum of Marine Biology and Aquarium, Pingtung, Taiwan, Republic of China. tehaochen@nmmba.gov.tw
authors.forename: Te-Hao
authors.initials: TH
authors.lastname: Chen
authors.forename: Ying-Min
authors.initials: YM
authors.lastname: Cheng
authors.forename: Jing-O
authors.initials: JO
authors.lastname: Cheng
authors.forename: Fung-Chi
authors.initials: FC
authors.lastname: Ko
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D015870:Gene Expression; D018384:Oxidative Stress; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000378:metabolism / Q000502:physiology; D011078:Polychlorinated Biphenyls / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D045332:Photosystem II Protein Complex; D014874:Water Pollutants, Chemical; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls