title: Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants.
abstract: Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants that contained the VPg gene with the correct sequence. They generated VPg mRNA, but VPg protein was not detected. Some plants were immune to PVY infection suggesting post-transcriptional gene silencing. However, the likely PVY VPg toxicity exerted at an early stage of transformed seeds development precludes its use for engineering pathogen-derived resistance.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Wojtal
authors.forename: Paulina
authors.initials: P
authors.lastname: Piontek
authors.forename: Renata
authors.initials: R
authors.lastname: Grzela
authors.forename: Artur
authors.initials: A
authors.lastname: Jarmołowski
authors.forename: Włodzimierz
authors.initials: W
authors.lastname: Zagórski
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Chroboczek
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000821:virology; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified / Q000235:genetics; D017800:Potyvirus / Q000235:genetics / Q000472:pathogenicity; D012333:RNA, Messenger / Q000032:analysis; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017361:Viral Nonstructural Proteins; C102206:genome-linked viral protein, potato virus Y