title: 1H, 15N, and 13C resonance assignments for a monomeric mutant of the HIV-1 capsid protein.
abstract: The mature fullerene cone-shaped capsid of the human immunodeficiency virus 1 is composed of about 1,500 copies of the capsid protein (CA). The CA is 231 residues long, and consists of two distinct structural domains, the N-terminal domain and the C-terminal domain (CTD), joined by a flexible linker. The wild type CA exhibits monomer-dimer equilibrium in solution through the CTD-CTD dimerization. This CTD-CTD interaction, together with other intermolecular interdomain interactions, plays significant roles during the assembly of the mature capsid. In addition, CA-CA interactions also play a role in the assembly of the immature virion. The CA also interacts with some host cell proteins within the viral replication cycle. Thus, the capsid protein has been of significant interest as a target for designing inhibitors of assembly of immature virions and mature capsids and inhibitors of its interactions with host cell proteins. However, the equilibrium exhibited by the wild-type CA protein between the monomeric and dimeric states, along with the inherent flexibility from the interdomain linker, have hindered attempts at structural determination by solution NMR and X-ray crystallography methods. In this study, we have utilized a CA protein with W184A and M185A mutations that abolish the dimerization of CA protein as well as its infectivity, but preserve most of the remaining properties of the wild type CA. We have determined the detailed solution structure of the monomeric W184A/M185A-CA protein using 3D-NMR spectroscopy. Here, we present the detailed sequence-specific NMR assignments for this protein.
authors.affiliation: UAB High-Field NMR Facility, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA. shinr@uab.edu
authors.forename: Ronald
authors.initials: R
authors.lastname: Shin
authors.forename: Ywh-Min
authors.initials: YM
authors.lastname: Tzou
authors.forename: Hing C
authors.initials: HC
authors.lastname: Wong
authors.forename: N Rama
authors.initials: NR
authors.lastname: Krishna
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry*; D002247:Carbon Isotopes; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D050505:Mutant Proteins / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons
chemical_list: D036022:Capsid Proteins; D002247:Carbon Isotopes; D050505:Mutant Proteins; D009587:Nitrogen Isotopes; D011522:Protons