title: Altered development of NKT cells, γδ T cells, CD8 T cells and NK cells in a PLZF deficient patient.
abstract: In mice, the transcription factor, PLZF, controls the development of effector functions in invariant NKT cells and a subset of NKT cell-like, γδ T cells. Here, we show that in human lymphocytes, in addition to invariant NKT cells, PLZF was also expressed in a large percentage of CD8+ and CD4+ T cells. Furthermore, PLZF was also found to be expressed in all γδ T cells and in all NK cells. Importantly, we show that in a donor lacking functional PLZF, all of these various lymphocyte populations were altered. Therefore, in contrast to mice, PLZF appears to control the development and/or function of a wide variety of human lymphocytes that represent more than 10% of the total PBMCs. Interestingly, the PLZF-expressing CD8+ T cell population was found to be expanded in the peripheral blood of patients with metastatic melanoma but was greatly diminished in patients with autoimmune disease.
authors.affiliation: Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America.
authors.forename: Maggie
authors.initials: M
authors.lastname: Eidson
authors.forename: Justin
authors.initials: J
authors.lastname: Wahlstrom
authors.forename: Aimee M
authors.initials: AM
authors.lastname: Beaulieu
authors.forename: Bushra
authors.initials: B
authors.lastname: Zaidi
authors.forename: Steven E
authors.initials: SE
authors.lastname: Carsons
authors.forename: Peggy K
authors.initials: PK
authors.lastname: Crow
authors.forename: Jianda
authors.initials: J
authors.lastname: Yuan
authors.forename: Jedd D
authors.initials: JD
authors.lastname: Wolchok
authors.forename: Bernhard
authors.initials: B
authors.lastname: Horsthemke
authors.forename: Dagmar
authors.initials: D
authors.lastname: Wieczorek
authors.forename: Derek B
authors.initials: DB
authors.lastname: Sant'Angelo
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000276:immunology / Q000378:metabolism; D055611:Natural Killer T-Cells / Q000378:metabolism*; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D060888:Real-Time Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000378:metabolism*
chemical_list: D051741:Kruppel-Like Transcription Factors; D000075722:Promyelocytic Leukemia Zinc Finger Protein; D016692:Receptors, Antigen, T-Cell, gamma-delta; C092037:ZBTB16 protein, human