title: Identification of heterotrimeric G protein α and β subunits in rice.
abstract: Like those in mammals, heterotrimeric G protein complexes have been implicated in signal transduction pathways in plants; however, the subunits themselves have not been isolated. In this study, the rice heterotrimeric G protein subunits α (Gα) and β (Gβ) were purified by affinity chromatography using anti-Gα and -Gβ antibodies and SDS-PAGE. Six and seven peptides, respectively, were identified by mass spectrometry and identified as the translation products of the Gα gene RGA1 and Gβ gene RGB1. During purification, the subunits dissociated easily from the G protein complex.
authors.affiliation: Department of Bioscience, Fukui Prefectural University, Yoshida-gun, Fukui, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Keiko
authors.initials: K
authors.lastname: Matsusita
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.forename: Yukimoto
authors.initials: Y
authors.lastname: Iwasaki
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D044385:GTP-Binding Protein alpha Subunits / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D044387:GTP-Binding Protein beta Subunits / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000737:chemistry
chemical_list: D000906:Antibodies; D044385:GTP-Binding Protein alpha Subunits; D044387:GTP-Binding Protein beta Subunits; D010940:Plant Proteins; D012117:Resins, Synthetic