title: Anti-Parkinsonian effects of Bacopa monnieri: insights from transgenic and pharmacological Caenorhabditis elegans models of Parkinson's disease.
abstract: Neurodegenerative Parkinson's disease (PD) is associated with aggregation of protein alpha synuclein and selective death of dopaminergic neurons, thereby leading to cognitive and motor impairment in patients. The disease has no complete cure yet; the current therapeutic strategies involve prescription of dopamine agonist drugs which turn ineffective after prolonged use. The present study utilized the powerful genetics of model system Caenorhabditis elegans towards exploring the anti-Parkinsonian effects of a neuro-protective botanical Bacopa monnieri. Two different strains of C. elegans; a transgenic model expressing "human" alpha synuclein [NL5901 (P(unc-54)::alphasynuclein::YFP+unc-119)], and a pharmacological model expressing green fluorescent protein (GFP) specifically in the dopaminergic neurons [BZ555 (P(dat-1)::GFP)] treated with selective catecholaminergic neurotoxin 6-hydroxy dopamine (6-OHDA), were employed for the study. B. monnieri was chosen for its known neuroprotective and cognition enhancing effects. The study examined the effect of the botanical, on aggregation of alpha synuclein, degeneration of dopaminergic neurons, content of lipids and longevity of the nematodes. Our studies show that B. monnieri reduces alpha synuclein aggregation, prevents dopaminergic neurodegeneration and restores the lipid content in nematodes, thereby proving its potential as a possible anti-Parkinsonian agent. These findings encourage further investigations on the botanical, and its active constituent compounds, as possible therapeutic intervention against Parkinson's disease.
authors.affiliation: Laboratory of Functional Genomics and Molecular Toxicology, Division of Toxicology, CSIR-Central Drug Research Institute, Lucknow 226001, UP, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Jadiya
authors.forename: Asif
authors.initials: A
authors.lastname: Khan
authors.forename: Shreesh Raj
authors.initials: SR
authors.lastname: Sammi
authors.forename: Supinder
authors.initials: S
authors.lastname: Kaur
authors.forename: Snober S
authors.initials: SS
authors.lastname: Mir
authors.forename: Aamir
authors.initials: A
authors.lastname: Nazir
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000978:Antiparkinson Agents / Q000737:chemistry / Q000494:pharmacology*; D034681:Bacopa / Q000737:chemistry*; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000378:metabolism; D019610:Cytoprotection; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008136:Longevity / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010300:Parkinson Disease / Q000378:metabolism* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D051844:alpha-Synuclein / Q000378:metabolism
chemical_list: D000978:Antiparkinson Agents; D018696:Neuroprotective Agents; D010936:Plant Extracts; D051844:alpha-Synuclein