title: Production of cloned transgenic cow expressing omega-3 fatty acids.
abstract: n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P<0.05) in both tissue and milk were observed.
authors.affiliation: The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.forename: Biao
authors.initials: B
authors.lastname: Duan
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Ting
authors.initials: T
authors.lastname: Yuan
authors.forename: Lian
authors.initials: L
authors.lastname: Xue
authors.forename: Daibang
authors.initials: D
authors.lastname: Ni
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Shuhua
authors.initials: S
authors.lastname: Dong
authors.forename: Zhuying
authors.initials: Z
authors.lastname: Wei
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
authors.forename: Liangxue
authors.initials: L
authors.lastname: Lai
authors.forename: Yifan
authors.initials: Y
authors.lastname: Dai
authors.forename: Guang-Peng
authors.initials: GP
authors.lastname: Li
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D015139:Blotting, Southern; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D004624:Embryo Transfer; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D017238:Genes, Helminth; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D008892:Milk / Q000378:metabolism*; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D014162:Transfection
chemical_list: D029742:Caenorhabditis elegans Proteins; D015525:Fatty Acids, Omega-3; C466741:fat-1 protein, C elegans; D044943:Fatty Acid Desaturases