title: Monoclonal antibody-based broad-specificity immunoassay for monitoring organophosphorus pesticides in environmental water samples.
abstract: The development of easy-to-use and rapid-monitoring immunoassay methods for organic environmental pollutants in a class-selective manner is a topic of considerable environmental interest. In this work, a heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) based on a monoclonal antibody (MAb) with broad-specificity for organophosphorus pesticides (OPs) was applied to the detection of O,O-diethyl and O,O-dimethyl OPs in water samples. The ciELISA conditions were carefully optimized to obtain a three to five-fold improvement of sensitivity for most OPs, and thirteen OPs were determined at concentrations ranging from 0.017 to 30 ng mL(-1). The determination of spiked environmental water samples showed average recoveries from 81.5% to 115.1%, with the coefficient of variation (CV) ranging from 6.1% to 20.9%, which showed satisfactory reproducibility of the developed ciELISA. To overcome the negative aspect of broad-specificity immunoassays not providing qualitative and quantitative analysis of individual OPs in blind samples, we used "percent inhibition rate" to make the developed ciELISA a semi-quantitative method, which allows the monitoring of positive samples from hundreds of negative samples. The determination of OPs in blind water samples by the developed ELISA with confirmation by HPLC-MS/MS analysis demonstrated that the assay is ideally suited as a screening method for OP residues prior to chromatographic analysis.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Zhen-Lin
authors.initials: ZL
authors.lastname: Xu
authors.forename: Dao-Ping
authors.initials: DP
authors.lastname: Zeng
authors.forename: Jin-Yi
authors.initials: JY
authors.lastname: Yang
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Shen
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Lei
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Sun
mesh_terms: D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057230:Limit of Detection; D009943:Organophosphorus Compounds / Q000032:analysis*; D010575:Pesticides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical