title: The effects of fractions from shiitake mushroom on composition and cariogenicity of dental plaque microcosms in an in vitro caries model.
abstract: The aim of the current study was to investigate the anticariogenic potential of the (sub)fractions obtained from the edible mushroom shiitake (Lentinula edodes) in in vitro caries model. We used a modified constant depth film fermentor (CDFF) with pooled saliva as the inoculum and bovine dentin as a substratum. The test compounds were low molecular weight fraction (MLMW) of the shiitake extract and subfractions 4 and 5 (SF4 and SF5) of this fraction. Chlorhexidine (CHX) and water served as a positive and a negative control, respectively. Dentin mineral loss was quantified (TMR), microbial shifts within the microcosms were determined (qPCR), and the acidogenicity of the microcosms was assessed (CIA). From the compounds tested, the SF4 of shiitake showed strong inhibiting effect on dentin demineralization and induced microbial shifts that could be associated with oral health. The acid producing potential was increased, suggesting uncoupling of the glycolysis of the microbiota by the exposure to SF4. In conclusion, the results suggest that SF4 of shiitake has an anticariogenic potential.
authors.affiliation: Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam-ACTA, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands. e.zaura@acta.nl
authors.forename: Egija
authors.initials: E
authors.lastname: Zaura
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Buijs
authors.forename: Michel A
authors.initials: MA
authors.lastname: Hoogenkamp
authors.forename: Lena
authors.initials: L
authors.lastname: Ciric
authors.forename: Adele
authors.initials: A
authors.lastname: Papetti
authors.forename: Caterina
authors.initials: C
authors.lastname: Signoretto
authors.forename: Monica
authors.initials: M
authors.lastname: Stauder
authors.forename: Peter
authors.initials: P
authors.lastname: Lingström
authors.forename: Jonathan
authors.initials: J
authors.lastname: Pratten
authors.forename: David A
authors.initials: DA
authors.lastname: Spratt
authors.forename: Michael
authors.initials: M
authors.lastname: Wilson
mesh_terms: D000818:Animals; D018441:Biofilms / Q000187:drug effects*; D002327:Cariostatic Agents / Q000494:pharmacology*; D002417:Cattle; D003731:Dental Caries / Q000517:prevention & control*; D003773:Dental Plaque / Q000378:metabolism* / Q000382:microbiology; D003804:Dentin / Q000737:chemistry / Q000187:drug effects; D008954:Models, Biological; D008970:Molecular Weight; D012463:Saliva / Q000382:microbiology; D020942:Shiitake Mushrooms / Q000737:chemistry*; D018709:Statistics, Nonparametric
chemical_list: D002327:Cariostatic Agents