title: Omethoate modulates some oxidant/antioxidant parameters in frogs (Rana ridibunda Pallas).
abstract: The aim of this study was to investigate the effect of an organophosphorus insecticide omethoate (OM), on certain oxidative stress biomarkers (malondialdehyde (MDA), reduced glutathione (GSH), glutathione-S-transferase (GST), glutathione reductase (GR), catalase (CAT)) in tongue, lung, stomach and muscle tissues of adult frogs (Rana ridibunda Pallas). Animals were exposed to 10 and 20 parts per million dosages of OM for 24, 48, 72 or 96 h. According to the results, MDA level increased significantly in lung and stomach tissues. GSH content fluctuated in lung and muscle while it elevated in tongue and stomach tissues. With regard to antioxidant enzymes (GST, GR and CAT), their activities reduced in tongue, while they increased in lung and fluctuated in stomach and muscle tissues. It can be concluded that exposure of frogs to OM are characterized by increased MDA levels and fluctuated enzyme activities and GSH contents. This may reflect the potential role of these parameters as useful biomarkers for assessment of OM toxicity.
authors.affiliation: Faculty of Medicine, Department of Medical Biology, Yuzuncu Yil University, Van, Turkey. h_ozkol@mynet.com
authors.forename: Halil
authors.initials: H
authors.lastname: Özkol
authors.forename: Yasin
authors.initials: Y
authors.lastname: Tuluce
authors.forename: Ismail
authors.initials: I
authors.lastname: Celik
authors.forename: Ismail
authors.initials: I
authors.lastname: Isik
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004117:Dimethoate / Q000031:analogs & derivatives* / Q000633:toxicity; D005753:Gastric Mucosa / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008168:Lung / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D010575:Pesticides / Q000633:toxicity; D011895:Rana ridibunda / Q000378:metabolism*; D013270:Stomach / Q000737:chemistry; D014059:Tongue / Q000737:chemistry / Q000378:metabolism; D018675:Toxicity Tests
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010575:Pesticides; C002302:dimethoxon; D008315:Malondialdehyde; D002374:Catalase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D004117:Dimethoate