title: In vitro studies and preliminary in vivo evaluation of silicified concentrated collagen hydrogels.
abstract: Hybrid and nanocomposite silica-collagen materials derived from concentrated collagen hydrogels were evaluated in vitro and in vivo to establish their potentialities for biological dressings. Silicification significantly improved the mechanical and thermal stability of the collagen network within the hybrid systems. Nanocomposites were found to favor the metabolic activity of immobilized human dermal fibroblasts while decreasing the hydrogel contraction. Cell adhesion experiments suggested that in vitro cell behavior was dictated by mechanical properties and surface structure of the scaffold. First-to-date in vivo implantation of bulk hydrogels in subcutaneous sites of rats was performed over the vascular inflammatory period. These materials were colonized and vascularized without inducing strong inflammatory response. These data raise reasonable hope for the future application of silica-collagen biomaterials as biological dressings.
authors.affiliation: UPMC Univ Paris 06; CNRS, Chimie de la Matière Condensée de Paris, Collège de France, 11 place Marcelin Berthelot, F-75005 Paris, France.
authors.forename: Martín F
authors.initials: MF
authors.lastname: Desimone
authors.forename: Christophe
authors.initials: C
authors.lastname: Hélary
authors.forename: Sandrine
authors.initials: S
authors.lastname: Quignard
authors.forename: Ivo B
authors.initials: IB
authors.lastname: Rietveld
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bataille
authors.forename: Guillermo J
authors.initials: GJ
authors.lastname: Copello
authors.forename: Gervaise
authors.initials: G
authors.lastname: Mosser
authors.forename: Marie-Madeleine
authors.initials: MM
authors.lastname: Giraud-Guille
authors.forename: Jacques
authors.initials: J
authors.lastname: Livage
authors.forename: Anne
authors.initials: A
authors.lastname: Meddahi-Pellé
authors.forename: Thibaud
authors.initials: T
authors.lastname: Coradin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D008954:Models, Biological; D053761:Nanocomposites / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D012822:Silicon Dioxide; D003094:Collagen