title: New polyene macrolide family produced by submerged culture of Streptomyces durmitorensis.
abstract: A new polyene macrolide family, closely related to the pentaene macrolide antibiotic roflamycoin, was isolated from the both fermentation broth and biomass of Streptomyces durmitorensis wild-type strain MS405. The main compound was identified by NMR and Fourier transform ion cyclotron resonance mass spectrometry as 32,33-didehydroroflamycoin (1; DDHR). Additional four structurally related compounds were determined solely by MS analysis. DDHR induces cell death by apoptosis in various cancer cell lines as demonstrated by DNA fragmentation. Striking feature of DDHR is its internal fluorescence allowing visualization of labeled plasma membranes and internal membrane structures.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic v.v.i., Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Stodůlková
authors.forename: Marek
authors.initials: M
authors.lastname: Kuzma
authors.forename: Ivana Bratic
authors.initials: IB
authors.lastname: Hench
authors.forename: Jan
authors.initials: J
authors.lastname: Cerný
authors.forename: Jarmila
authors.initials: J
authors.lastname: Králová
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Milada
authors.initials: M
authors.lastname: Chudíčková
authors.forename: Miloje
authors.initials: M
authors.lastname: Savic
authors.forename: Lidija
authors.initials: L
authors.lastname: Djokic
authors.forename: Branka
authors.initials: B
authors.lastname: Vasiljevic
authors.forename: Miroslav
authors.initials: M
authors.lastname: Flieger
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D015394:Molecular Structure; D011090:Polyenes / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D018942:Macrolides; D011090:Polyenes