title: [Effect of danggui buxue decoction on the hemopoiesis reconstruction of mouse transplanted by the muscle satellite cell receptor].
abstract: OBJECTIVE
  To study the effects of Danggui Buxue Decoction on the hematopoietic reconstruction of mice transplanted by muscle satellite cells ( MSCs).
  
  
  METHODS
  MSCs were procured from newly born male mice of the homologous series. The female Kunming receptor mice irradiated with 8Gy137Cs-gamma ray were randomly divided into six groups, i. e., the blank control group, the transplanted MSC group, four groups intervened by different doses of Danggui Buxue Decoction after transplanted MSC (gastrogavage by one, three, five, and ten times of clinically equivalent dose for seven days, as the DGBX 1 group, the DGBX 2 group, the DGBX 3 group, and the DGBX 4 group). After transplantation changes of splenic colony forming unit spleen (CFU-S), white blood cells (WBC), hemoglobin (Hb), and platelet (PLT) in the peripheral blood were observed in pos-transplanted 1-, 2-, and 3-week receptor mice. The 3-week survival rate was calculated. The source of hematopoietic reconstruction was identified using PCR.
  
  
  RESULTS
  The desmin protein was positive in the cultured MSCs. WBC of each transplanted MSC group obviously increased at the 2nd week (P<0.05). Hb obviously increased in the DGBX 2 group, the DGBX 3 group, and the DGBX 4 group (P<0.05). WBC and Hb obviously increased in the DGBX 3 group and the DGBX 4 group when compared with the transplanted MSC group at the 3rd week (P<0.05). The recovery of PLT was significant in the DGBX 4 group (P<0.05). Compared with the blank control group at the 2nd week, CFU-S obviously increased in the DGBX 3 group and the DGBX 4 group (P<0.05). PCR results of Y chromosome in the survived transplant mice indicated that the hematopoietic cells of reconstruction female receptor mice were originated from male donors.
  
  
  CONCLUSIONS
  The hematopoiesis of mice transplanted by muscle satellite cells could be constructed after intervention of Danggui Buxue Decoction.
authors.affiliation: Department of Histology and Embryology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193.
authors.forename: Xiao-ling
authors.initials: XL
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Ya-ni
authors.initials: YN
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D032496:Satellite Cells, Skeletal Muscle / Q000187:drug effects* / Q000637:transplantation
chemical_list: D004365:Drugs, Chinese Herbal; C477279:danggui buxue decoction