title: Intraspecific genetic variability of Edwardsiella tarda strains from cultured turbot.
abstract: Edwardsiella tarda is an enterobacterial fish pathogen that causes mortality in various fish species worldwide. In this study, we analyzed the intraspecific variability in a collection of E. tarda strains isolated from turbot. To do this we employed 4 polymerase chain reaction (PCR)-based methods: (1) random amplified polymorphic DNA (RAPD), (2) enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR), (3) repetitive extragenic palindromic-PCR (REP-PCR) and (4) BOX-PCR. E. tarda isolates from different hosts were also included for comparison. E. tarda strains from turbot showed high molecular homogeneity when RAPD (primers P3 and P6), ERIC-PCR and BOX-PCR were employed. However, with regard to the REP-PCR and RAPD (primers P4 and P5) techniques, different genetic groups could be established within these isolates using either technique. The 2 RAPD types presented an 85% similarity, while those obtained with REP-PCR showed 74% similarity. Based on the results obtained, although a high genetic homogeneity was found in turbot isolates, the RAPD test (with primers P4 and P5) and REP-PCR were capable of discrimination within these strains, and they are therefore considered the most appropriate typing methods for studies of edwardsiellosis in turbot.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Biología-CIBUS-Instituto de Acuicultura, Universidad de Santiago de Compostela, Santiago de Compostela, 15782 Spain. nuria.castro@usc.es
authors.forename: N
authors.initials: N
authors.lastname: Castro
authors.forename: A E
authors.initials: AE
authors.lastname: Toranzo
authors.forename: A
authors.initials: A
authors.lastname: Bastardo
authors.forename: J L
authors.initials: JL
authors.lastname: Barja
authors.forename: B
authors.initials: B
authors.lastname: Magariños
mesh_terms: D000818:Animals; D020609:Edwardsiella tarda / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D005393:Fish Diseases / Q000382:microbiology*; D005412:Flatfishes; D014644:Genetic Variation; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods / Q000662:veterinary