title: Less expression of prohibitin is associated with increased caspase-3 expression and cell apoptosis in renal interstitial fibrosis rats.
abstract: OBJECTIVE
  Prohibitin (PHB), a ubiquitous protein, is involved in a variety of molecular functions. Renal interstitial fibrosis (RIF) is a hallmark of common progressive chronic diseases that lead to renal failure. This study was performed to investigate whether PHB was associated with caspase-3 expression/cell apoptosis in RIF rats.
  
  
  METHODS
  Twenty-four male Wistar rats were randomly divided into two groups: sham operation group (SHO) and model group subjected to unilateral ureteral obstruction (GU), n = 12, respectively. The model was established by left ureteral ligation. Renal tissues were collected at 14 days and 28 days after surgery. RIF index, cell apoptosis index, protein expression of PHB, transforming growth factor-βl (TGF-β1), collagen-IV (Col-IV), fibronectin (FN) or caspase-3 in renal interstitium, and mRNA expression of PHB in renal tissue were detected.
  
  
  RESULTS
  Compared with that in the SHO group, the PHB expression (mRNA and protein) was significantly reduced (P < 0.01). Protein expressions of TGF-β1, Col-IV, FN and caspase-3, and RIF index or cell apoptosis index in GU group were markedly elevated compared with those in SHO group (all P < 0.01). The protein expression of PHB had a negative correlation with the protein expression of TGF-β1, Col-IV, FN or caspase-3, and RIF index or cell apoptosis index (each P < 0.01).
  
  
  CONCLUSIONS
  Less expression of PHB is associated with increased caspase-3 expression/cell apoptosis in RIF rats. However, further research is needed to determine the effect of PHB on caspase-3 expression/cell apoptosis and to determine the potential of PHB as a therapeutic target.
authors.affiliation: Department of Pediatrics, The First Affiliated Hospital of GuangXi Medical University, NanNing, China.
authors.forename: Tian-Biao
authors.initials: TB
authors.lastname: Zhou
authors.forename: Yuan-Han
authors.initials: YH
authors.lastname: Qin
authors.forename: Chun
authors.initials: C
authors.lastname: Zhou
authors.forename: Feng-Ying
authors.initials: FY
authors.lastname: Lei
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Su
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism*; D024141:Collagen Type IV / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation; D005353:Fibronectins / Q000378:metabolism; D005355:Fibrosis; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D007674:Kidney Diseases / Q000201:enzymology* / Q000209:etiology / Q000235:genetics / Q000473:pathology; D008297:Male; D000091402:Prohibitins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D015854:Up-Regulation; D014517:Ureteral Obstruction / Q000150:complications
chemical_list: D024141:Collagen Type IV; D005353:Fibronectins; C000717332:Phb protein, rat; D000091402:Prohibitins; D012333:RNA, Messenger; D012097:Repressor Proteins; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; C505410:Casp3 protein, rat; D053148:Caspase 3