title: Mechanoformation of neutral giant phospholipid vesicles in high ionic strength solution.
abstract: We present new and simple method for formation of giant unilamellar vesicles (GUVs) in high ionic strength solutions, such as phosphate buffered saline (PBS). Mechanoformation method is an alternative method to electroformation method. The advantage of the mechanoformation procedure is that there are no limitations with respect to the ionic strength of the aqueous solutions, because there is no applied electric potential thus no current flow through the formation cell and no electrolysis is induced.
authors.affiliation: University of Ljubljana, Faculty of Electrical Engineering, Tržaška 25, Ljubljana, Slovenia. janez.pavlic@fe.uni-lj.si
authors.forename: Janez I
authors.initials: JI
authors.lastname: Pavlič
authors.forename: Julia
authors.initials: J
authors.lastname: Genova
authors.forename: George
authors.initials: G
authors.lastname: Popkirov
authors.forename: Veronika
authors.initials: V
authors.lastname: Kralj-Iglič
authors.forename: Aleš
authors.initials: A
authors.lastname: Iglič
authors.forename: Marin D
authors.initials: MD
authors.lastname: Mitov
mesh_terms: D008722:Methods; D009994:Osmolar Concentration; D010743:Phospholipids / Q000737:chemistry*; D012996:Solutions; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D010743:Phospholipids; D012996:Solutions; D053835:Unilamellar Liposomes