title: Oridonin induced the apoptosis of PC-3 cells and its mechanism.
abstract: OBJECTIVE
  To observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.
  
  
  METHODS
  PC-3 cells were treated with different concentrations of oridonin. MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells. The percentage of earlier apoptosis cells was analyzed by flow cytometry. The protein expression of caspase-3, Bcl-2, and Bax in the PC-3 cells was detected by Western blot.
  
  
  RESULTS
  Oridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner, and the IC50 of PC-3 cells was 10.29 μmol/L. HochesT(3)3258 staining and flow cytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P<0.05). Oridonin down-regulated Bcl-2, up-regulated Bax protein, and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.
  
  
  CONCLUSIONS
  The apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
authors.affiliation: Department of Urology, Central Hospital of Xiangtan, Xiangtan Hunan 411100,China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Luoyan
authors.initials: L
authors.lastname: Yang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D045786:Diterpenes, Kaurane / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D011467:Prostate / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D045786:Diterpenes, Kaurane; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C011959:oridonin; C505407:CASP3 protein, human; D053148:Caspase 3