title: Consequences of PPAR(α) Invalidation on Glutathione Synthesis: Interactions with Dietary Fatty Acids.
abstract: Glutathione (GSH) derives from cysteine and plays a key role in redox status. GSH synthesis is determined mainly by cysteine availability and γ-glutamate cysteine ligase (γGCL) activity. Because PPARα activation is known to control the metabolism of certain amino acids, GSH synthesis from cysteine and related metabolisms were explored in wild-type (WT) and PPARα-null (KO) mice, fed diets containing either saturated (COCO diet) or 18 : 3 n-3, LIN diet. In mice fed the COCO diet, but not in those fed the LIN diet, PPARα deficiency enhanced hepatic GSH content and γGCL activity, superoxide dismutase 2 mRNA levels, and plasma uric acid concentration, suggesting an oxidative stress. In addition, in WT mice, the LIN diet increased the hepatic GSH pool, without effect on γGCL activity, or change in target gene expression, which rules out a direct effect of PPARα. This suggests that dietary 18 : 3 n-3 may regulate GSH metabolism and thus mitigate the deleterious effects of PPARα deficiency on redox status, without direct PPARα activation.
authors.affiliation: INRA, UMR914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Najoua
authors.initials: N
authors.lastname: Guelzim
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Huneau
authors.forename: Véronique
authors.initials: V
authors.lastname: Mathé
authors.forename: Annie
authors.initials: A
authors.lastname: Quignard-Boulangé
authors.forename: Pascal G
authors.initials: PG
authors.lastname: Martin
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.forename: Dominique
authors.initials: D
authors.lastname: Hermier