title: Endothelial nitric oxide synthase T(-786)C polymorphism in children and adolescents with type 1 diabetes and impaired endothelium-dependent dilatation.
abstract: OBJECTIVE
  This study aimed to investigate the association of endothelial nitric oxide synthase (eNOS) polymorphisms with impaired endothelium-dependent dilatation (EDD) in a cohort of children and adolescents with type 1 diabetes.
  
  
  METHODS
  Ninety-seven children and adolescents with type 1 diabetes underwent ultrasound assessment of EDD. The association of various factors with EDD was analyzed with multivariate linear regression analysis. Genotypes were determined for the eNOS T(-786)C and 4ab polymorphisms, and their association with EDD was tested with logistic regression analysis.
  
  
  RESULTS
  Thirty-three percent of children had impaired EDD. EDD was independently associated with A1c (p = 0.0005) and inversely correlated with A1c (p = 0.0037, OR = 2.14) using logistic regression analysis. The presence of any C allele at eNOS (-786) was significantly more frequent in patients with impaired EDD (OR = 2.97, 95% CI = 1.08-8.87, p = 0.03). Logistic regression analysis revealed OR of 3.09 for impaired EDD for patients with any C allele as compared to TT patients when controlling for all other covariates (p = 0.048).
  
  
  CONCLUSIONS
  A third of children and adolescents with type 1 diabetes had impaired EDD independently associated with A1c. The presence of any C at (-786) of the eNOS gene conveyed a significantly increased independent risk for impaired EDD.
authors.affiliation: Department of Pediatric Endocrinology, Diabetes and Metabolic Diseases, University Children's Hospital, UMC, Bohoričeva 20, Ljubljana, Slovenia.
authors.forename: Nina
authors.initials: N
authors.lastname: Battelino
authors.forename: Miran
authors.initials: M
authors.lastname: Sebestjen
authors.forename: Irena
authors.initials: I
authors.lastname: Keber
authors.forename: Rok
authors.initials: R
authors.lastname: Blagus
authors.forename: Tinka
authors.initials: T
authors.lastname: Hovnik
authors.forename: Natasa
authors.initials: N
authors.lastname: Bratina
authors.forename: Tadej
authors.initials: T
authors.lastname: Battelino
mesh_terms: D000293:Adolescent; D001916:Brachial Artery / Q000503:physiopathology*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D004730:Endothelium, Vascular; D005260:Female; D005838:Genotype; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D011110:Polymorphism, Genetic; D014661:Vasoconstriction; D014664:Vasodilation / Q000502:physiology*
chemical_list: D006442:Glycated Hemoglobin; D052250:Nitric Oxide Synthase Type III; C517652:hemoglobin A1c protein, human; C496314:NOS3 protein, human