title: Extracellular acidosis promotes neutrophil transdifferentiation to MHC class II-expressing cells.
abstract: Inflammation in peripheral tissues is usually associated with local acidosis. In the present study, we demonstrate that extracellular acidification enhances GM-CSF- and IFN-γ-induced expression of HLA-DR, CD80 and CD86 in human neutrophils (neutrophil transdifferentiation), and potentiates antigen-capturing capacities (both endocytosis and phagocytosis) of the transdifferentiated cells. Furthermore, in acidic conditions the transdifferentiated neutrophils have stronger antigen-presenting capacity, inducing more intense proliferation of autologous T lymphocytes in the presence of staphylococcal enterotoxin A. Thus, extracellular acidosis can represent a factor that promotes neutrophil transdifferentiation and potentiates the functional abilities of the transdifferentiated cells in inflammatory foci in vivo.
authors.affiliation: Department of Biological and Medical Chemistry, Faculty of Fundamental Medicine, Moscow State University, Moscow, Russia.
authors.forename: Boris K
authors.initials: BK
authors.lastname: Pliyev
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Sumarokov
authors.forename: Lyudmila I
authors.initials: LI
authors.lastname: Buriachkovskaia
authors.forename: Mikhail
authors.initials: M
authors.lastname: Menshikov
mesh_terms: D000138:Acidosis / Q000276:immunology* / Q000473:pathology; D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D054338:Cell Transdifferentiation / Q000187:drug effects / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D007371:Interferon-gamma / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D010587:Phagocytosis
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor