title: Enhanced homing of mesenchymal stem cells to the ischemic myocardium by ultrasound-targeted microbubble destruction.
abstract: In recent years, ultrasound-targeted microbubble destruction (UTMD) has been utilised for the targeted delivery of stem cells. We tested the effects of the myocardial micro-environment changes induced by UTMD on promoting the homing of mesenchymal stem cells (MSCs) to the ischemic myocardium. Dogs were randomly divided into two groups and treated with or without UTMD after the establishment of myocardial infarction models. 4,6-diamino-2-phenyl indole (DAPI) labelled MSCs were transplanted via coronary injections 2 weeks after myocardial infarction in both groups. The results from real-time PCR and western blot analyses indicated that the expression of various cytokines in UTMD-treated dogs was much higher than that observed in non-treated dogs. Histopathological findings demonstrate that ultrasound at a frequency of 1MHz and an intensity of 1.0W/cm(2) provoked inflammatory reactions with mild myocardial damage. Myocardial microenvironment changes caused by UTMD may promote the homing of MSCs to the ischemic myocardium. This non-invasive technique may be a promising method for cardiac cell transplantation therapy.
authors.affiliation: Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Shigen
authors.initials: S
authors.lastname: Zhong
authors.forename: Shiyu
authors.initials: S
authors.lastname: Shu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.forename: Weiying
authors.initials: W
authors.lastname: Zhong
authors.forename: Haitao
authors.initials: H
authors.lastname: Ran
authors.forename: Yuanyi
authors.initials: Y
authors.lastname: Zheng
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Yin
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Ling
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004285:Dogs; D007211:Indoles; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D045423:Microbubbles; D017202:Myocardial Ischemia / Q000473:pathology*; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D014465:Ultrasonics
chemical_list: D007211:Indoles; C007293:DAPI