title: [Expression of cytokeratin 17 in oral squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the expression of cytokeratin 17 (CK17) in oral squamous cell carcinoma (OSCC) as well as its clinical significance.
  
  
  METHODS
  Detection of the mRNA level and protein expression of CK17 in the in vitro cellular carcinogenesis model of OSCC, some OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction (PCR), Western blot and immunohistochemistry, respectively.
  
  
  RESULTS
  Increased CK17 mRNA level was observed in the HB56 and OSC cell lines compared with the HIOEC using real-time PCR technique. Western blot showed increased CK17 protein expression in all the cell lines compared with the HIOEC. Increased CK17 mRNA and immunoreaction levels were also observed in the cancerous tissue specimens from OSCC patients compared with normal adjacent tissues (P<0.01).
  
  
  CONCLUSIONS
  The significantly increased CK17 gene may be associated with the tumorigenesis and development of OSCC.
authors.affiliation: Dept. of Stomatology, School of Medicine, Huzhou Normal University, Huzhou 313000, China.
authors.forename: Kuijie
authors.initials: K
authors.lastname: Wei
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Laiping
authors.initials: L
authors.lastname: Zhong
authors.forename: Dongxia
authors.initials: D
authors.lastname: Ye
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhiyuan
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053537:Keratin-17; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms; D012333:RNA, Messenger
chemical_list: D053537:Keratin-17; D012333:RNA, Messenger