title: Neuropeptide RNA localization in tissue sections.
abstract: In situ hybridization has become a routine technique to provide insights into RNA localization. However, different protocols exist for multiple purposes, and it is, therefore, important to clearly define specific needs to choose the most suitable one(s). For instance, in situ hybridization can target different types of RNA, including mRNA or small noncoding RNA such as micro RNA (miRNA). Detection protocols are developed for light or electron microscopy and can be combined with immunocytochemistry to study RNA coexpression with proteins or peptides. In this chapter, we present some protocols to illustrate the diversity of in situ hybridization methods. We focus on the detection of mRNA or miRNA and show that the protocols are quite similar but use dedicated probe types, namely, oligo- or riboprobes and locked nucleic-acid probes.
authors.affiliation: INSERM U862, University of Bordeaux, Bordeaux, France. marc.landry@u-bordeaux2.fr
authors.forename: Marc
authors.initials: M
authors.lastname: Landry
authors.forename: Shérine Abdel
authors.initials: SA
authors.lastname: Salam
authors.forename: Marie
authors.initials: M
authors.lastname: Moftah
mesh_terms: D000818:Animals; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics; D009479:Neuropeptides / Q000235:genetics*; D015347:RNA Probes / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D035683:MicroRNAs; D009479:Neuropeptides; D015347:RNA Probes; D012333:RNA, Messenger