title: Anti-inflammatory effects of Clematis chinensis Osbeck extract(AR-6) may be associated with NF-κB, TNF-α, and COX-2 in collagen-induced arthritis in rat.
abstract: The root of Clematis chinensis Osbeck has been used widely in rheumatoid arthritis in Chinese traditional medicine, and AR-6 is a triterpene saponin isolated from it. In this present study, we investigated the in vivo effects of oral AR-6 in chronic rat with collagen-induced arthritis (CIA) and possible molecular mechanism. CIA was induced by immunizing 56 female Sprague-Dawley (SD) rats with chicken typeIIcollagen (CII). Following eighteen days, the immunization rats with CIA were treated with AR-6 (32, 16, 8 mg/kg), cyclophosphamide (7 mg/kg), and TGP (Total Glucosides of Paeonia) (180 mg/kg) for 7 days, and rats without CIA were given the same volume of purified water. TNF-α and IL-1β levels in peripheral blood will be measured by ELISA, and Western blot analysis will be used to detect the expression of NF-κB p65 subunits, TNF-α and COX-2, in synovial membrane. We found that therapeutic treatment with AR-6 markedly improves the paw swelling and histopathological changes. Moreover, the serum levels of pro-inflammatory cytokines TNF-α and IL-1β were markedly lowered, and the expression of NF-κB p65 subunits, TNF-α and COX-2, in the synovial membrane of CIA rats was significantly inhibited in the AR-6-treated groups. These results enable to prove that AR-6 has a potential anti-inflammatory effect in CIA rats, and its mechanism may relate to the inhibition of the expression of NF-κB p65 subunits, TNF-α and COX-2.
authors.affiliation: Department of Physiology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Pathirage Kamal
authors.initials: PK
authors.lastname: Perera
authors.forename: Yun-Man
authors.initials: YM
authors.lastname: Li
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Fang
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Liu
authors.forename: Feng-Wen
authors.initials: FW
authors.lastname: Li
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000201:enzymology / Q000276:immunology; D015153:Blotting, Western; D039324:Clematis / Q000737:chemistry; D024043:Collagen Type II; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D003520:Cyclophosphamide / Q000494:pharmacology; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005960:Glucosides / Q000494:pharmacology; D018836:Inflammation Mediators / Q000097:blood / Q000378:metabolism*; D053583:Interleukin-1beta / Q000097:blood; D029596:Paeonia; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D013997:Time Factors; D051996:Transcription Factor RelA / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*
chemical_list: D000893:Anti-Inflammatory Agents; D024043:Collagen Type II; D005960:Glucosides; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D010936:Plant Extracts; C498145:Rela protein, rat; D051996:Transcription Factor RelA; D014409:Tumor Necrosis Factor-alpha; D003520:Cyclophosphamide; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat