title: Taurine suppresses oxidative stress-potentiated expression of lectin-like oxidized low-density lipoprotein receptor and restenosis in balloon-injured rabbit iliac artery.
abstract: 1. In endothelial cells, the major receptor for the binding and internalization of oxidized low-density lipoprotein (LDL) is the lectin-like oxidized LDL receptor (LOX-1). The aim of the present study was to investigate the effects of taurine on intimal thickening and LOX-1 expression under normal and oxidative conditions. 2. The iliac artery of rabbits were subjected to balloon injury and oxidative stress was induced by 14 days treatment of rabbits with 75 mg/kg, s.c., buthionine sulfoximine (BSO), a specific inhibitor of glutathione synthesis. Taurine was administered in drinking water (1%, w/v) for 14 days in the presence (BSO + Taurine group) and in the absence of BSO treatment (Taurine group). In taurine and placebo groups, rabbits were injected with 4 mL, s.c., 0.9% NaCl (vehicle for BSO) for 14 days. 3. Taurine (1% in drinking water, w/v) preserved plasma levels of anti-oxidants and lowered the increased blood pressure induced by BSO. The stenosis rate of 29.92% in the placebo group increased to 72.20% in the BSO group, which was significantly reduced to 42.21% by taurine (P < 0.001; n = 5). Localization of LOX-1 to the intima and media of the iliac artery was demonstrated in the present study. Taurine treatment reduced the BSO-induced increase in LOX-1 expression at both the protein and mRNA levels (P < 0.05 and P < 0.01, respectively). 4. The results demonstrate that the stenosis rate and LOX-1 expression correlate well with oxidative status. Manipulation of LOX-1 expression by taurine may have therapeutic benefits in preventing restenosis.
authors.affiliation: Departments of Pharmacology, Faculty of Pharmacy Radiology, Faculty of Medicine, Ege University, Izmir, Turkey. goksel.gokce@ege.edu.tr
authors.forename: G
authors.initials: G
authors.lastname: Gokce
authors.forename: G
authors.initials: G
authors.lastname: Ozsarlak-Sozer
authors.forename: I
authors.initials: I
authors.lastname: Oran
authors.forename: G
authors.initials: G
authors.lastname: Oktay
authors.forename: S
authors.initials: S
authors.lastname: Ozkal
authors.forename: Z
authors.initials: Z
authors.lastname: Kerry
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis; D050197:Atherosclerosis / Q000139:chemically induced / Q000517:prevention & control; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007083:Iliac Artery / Q000293:injuries*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D011817:Rabbits; D051127:Scavenger Receptors, Class E / Q000096:biosynthesis*; D013654:Taurine / Q000494:pharmacology*; D017539:Tunica Intima / Q000187:drug effects / Q000378:metabolism; D017540:Tunica Media / Q000187:drug effects / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004791:Enzyme Inhibitors; D051127:Scavenger Receptors, Class E; D013654:Taurine; D019328:Buthionine Sulfoximine