title: [Investigation of fatty acid profile for diagnostic purposes in mouse melanoma].
abstract: The aim of this study was to investigate whether fatty acid profile is a suitable marker for diagnostic purposes in mouse melanoma. Twelve C57Bl/6 male mice were implanted with B16 mouse melanoma cells (106 cells/animal) orthotopically (subcutaneously). After the implantation 4-4 animals were bled by cardiac puncture following narcosis, at days 7, 14, and 21. In order to investigate fatty acid profiles a method based on extraction and HPLC-MS was developed. Signal intensities of 14 fatty acids were determined by mass spectrometry in tumor-free animals as well as tumor bearing animals at the three time points. Mathematical analysis showed non-significant profile changes when control (tumor-free) animals were compared to tumor-implanted ones as well as during tumor progression on week 1, 2 and 3. In case of three fatty acids (myristic acid, palmitoleic acid and eicosadienoic acid) a trend was observed during tumor progression but its statistical significance cannot be evaluated without further investigations. The fatty acid profile cannot be used for early diagnoses in mouse melanoma.
authors.affiliation: Onkológiai Osztály, Bajcsy -Zsilinszky Kórház, Budapest, Hungary. andras.telekes@bajcsy.hu
authors.forename: András
authors.initials: A
authors.lastname: Telekes
authors.forename: Erzsébet
authors.initials: E
authors.lastname: Rásó
authors.forename: Károly
authors.initials: K
authors.lastname: Vékey
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D018450:Disease Progression; D004537:Eicosanoic Acids / Q000097:blood; D005227:Fatty Acids / Q000097:blood*; D005229:Fatty Acids, Monounsaturated / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D008546:Melanoma, Experimental / Q000097:blood* / Q000175:diagnosis*; D051379:Mice; D008810:Mice, Inbred C57BL; D019814:Myristic Acid / Q000097:blood; D011237:Predictive Value of Tests; D013997:Time Factors
chemical_list: D004537:Eicosanoic Acids; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D019814:Myristic Acid; C008757:palmitoleic acid