title: Hyaline droplet accumulation in kidney of rats treated with hexachloro-1:3-butadiene: influence of age, dose and time-course.
abstract: The present research investigates the occurrence of hyaline droplet (HD) accumulation related to age, dose and time after treatment in male Wistar rats given a single i.p. injection of hexachloro-1:3-butadiene (HCBD). In the study on age, rats from 1 to 12 months of age were treated with 100 mg kg(-1) body weight (b.w.) HCBD dose. Rats treated at 2 months of age showed a greater accumulation of HD than the other age groups; HD accumulation was not observed in 1-month-old rats. In the dose-response study, the treatment with 25, 50 and 100 mg kg(-1) b.w. at 2 months of age caused HD accumulation in the proximal convoluted tubule at all doses, with the 100 mg kg(-1) b.w. group slightly more affected. Finally, in the time-course study, rats treated with a 100 mg kg(-1) b.w. dose at 2 months of age and sacrificed at 6, 12, 24, 48, 72 and 96 h post-dosing showed a time-related HD accumulation in terms of incidence and severity, after 6 h, with a peak at 24 and 48 h and decreasing at 72 and 96 h. The present results show that HD accumulation is an early finding, and is unrelated to dose level and particularly evident in rats of 2 month of age. These findings in male rats treated with HCBD emphasize the importance of considering the age of rats at the start of a study. The more sensitive model was used in the detection of nephrotoxic effects of chemicals.
authors.affiliation: GlaxoSmithKline, Pathology Department, Ware, UK.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Cristofori
authors.forename: Rossella
authors.initials: R
authors.lastname: Defazio
authors.forename: Arianna
authors.initials: A
authors.lastname: Chiusolo
authors.forename: Michele
authors.initials: M
authors.lastname: Mongillo
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: Bartolucci
authors.forename: Federica
authors.initials: F
authors.lastname: Chiara
authors.forename: Andrea
authors.initials: A
authors.lastname: Trevisan
mesh_terms: D000367:Age Factors; D000818:Animals; D002070:Butadienes / Q000633:toxicity*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000633:toxicity*; D006818:Hyalin / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D002070:Butadienes; D005659:Fungicides, Industrial; C001335:hexachlorobutadiene