title: Biochemical and histological investigation of famotidine effect on postischemic reperfusion injury in the rat ovary.
abstract: OBJECTIVE
  In this study, an investigation was performed on the ovarian tissue of rats subjected to ischemia-reperfusion for the effect of famotidine on certain parameters of oxidation-antioxidation, cell DNA damage, and histological appearance.
  
  
  METHODS
  The effects of famotidine on certain parameters of oxidation-antioxidation (total glutathione [tGSH], superoxide dismutase [SOD], malondialdehyde) and cellular DNA injury in the ovarian tissue of rats subjected to ischemia-reperfusion were investigated and underwent histological examination.
  
  
  RESULTS
  The results show levels of 5.2 ± 0.6 nmol/g protein for tGSH, 8.3 ± 0.8 U/g for SOD activity, and 7.7 ± 0.9 μmol/g protein for malondialdehyde (P < .0001 when compared with controls) in ovarian tissue subjected to ischemia-reperfusion following famotidine treatment. The tGSH levels in control rats and in a healthy animal group were, respectively, 1.76 ± 0.7 and 5.5 ± 0.3 nmol/g protein (P < .0001). The SOD activity was 3.2 ± 0.9 U/g in control and 9.2 ± 0.6 U/g in healthy animal tissues. The differences between the values in the treatment and the control group, and between the healthy animal group and the control group were both highly significant (P < .0001). It was also observed that famotidine prevented, to a significant extent, an increase in the level of 8-hydroxy-2-deoxyguanine/guanine, a DNA damage product, as compared with the control group.
  
  
  CONCLUSIONS
  These biochemical and histological results show that famotidine protects the ovarian tissue from ischemia-reperfusion injury.
authors.affiliation: Department of Pathology, Education and Research Hospital, Erzurum, Turkiye.
authors.forename: Ali
authors.initials: A
authors.lastname: Kurt
authors.forename: Unal
authors.initials: U
authors.lastname: Isaoglu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yilmaz
authors.forename: Muhammet
authors.initials: M
authors.lastname: Calik
authors.forename: Beyzagul
authors.initials: B
authors.lastname: Polat
authors.forename: Hamit
authors.initials: H
authors.lastname: Hakan
authors.forename: Metin
authors.initials: M
authors.lastname: Ingec
authors.forename: Halis
authors.initials: H
authors.lastname: Suleyman
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects; D015738:Famotidine / Q000494:pharmacology* / Q000627:therapeutic use*; D005260:Female; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
chemical_list: D015738:Famotidine