title: Rapid screening method for compounds that affect the growth and germination of Candida albicans, using a real-time PCR thermocycler.
abstract: We propose a screening method for compounds affecting growth and germination in Candida albicans using a real-time PCR thermocycler to quantify green fluorescent protein (GFP) fluorescence. Using P(ACT1)-GFP and P(HWP1)-GFP reporter strains, the effects of a wide range of compounds on growth and hyphal formation were quantitatively assessed within 3 h after inoculation.
authors.affiliation: Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands.
authors.forename: Lucja M
authors.initials: LM
authors.lastname: Jarosz
authors.forename: Bastiaan P
authors.initials: BP
authors.lastname: Krom
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000935:Antifungal Agents; D049452:Green Fluorescent Proteins