title: The Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation.
abstract: Induction of secreted acid phosphatase (APase) is a universal response of higher plants to phosphate (Pi) limitation. These enzymes are thought to scavenge Pi from organophosphate compounds in the rhizosphere and thus to increase Pi availability to plants when Pi is deficient. The tight association of secreted APase with the root surface may make plants more efficient in the utilization of soil Pi around root tissues, which is present in organophosphate forms. To date, however, no systematic molecular, biochemical, and functional studies have been reported for any of the Pi starvation-induced APases that are associated with the root surface after secretion. In this work, using genetic and molecular approaches, we identified Arabidopsis (Arabidopsis thaliana) Purple Acid Phosphatase10 (AtPAP10) as a Pi starvation-induced APase that is predominantly associated with the root surface. The AtPAP10 protein has phosphatase activity against a variety of substrates. Expression of AtPAP10 is specifically induced by Pi limitation at both transcriptional and posttranscriptional levels. Functional analyses of multiple atpap10 mutant alleles and overexpressing lines indicated that AtPAP10 plays an important role in plant tolerance to Pi limitation. Genetic manipulation of AtPAP10 expression may provide an effective means for engineering new crops with increased tolerance to Pi deprivation.
authors.affiliation: The Ministry of Education Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Liangsheng
authors.initials: L
authors.lastname: Wang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Qian
authors.forename: Wanli
authors.initials: W
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Lingling
authors.initials: L
authors.lastname: Huang
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Huifen
authors.initials: H
authors.lastname: Zhu
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wu
authors.forename: Daowen
authors.initials: D
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010710:Phosphates / Q000172:deficiency* / Q000494:pharmacology; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D013499:Surface Properties / Q000187:drug effects
chemical_list: D029681:Arabidopsis Proteins; D006023:Glycoproteins; D010710:Phosphates; D049452:Green Fluorescent Proteins; C091137:purple acid phosphatase; D000135:Acid Phosphatase