title: Determination of β haplotypes in patients with sickle-cell anemia in the state of Rio Grande do Norte, Brazil.
abstract: β(S) haplotypes were studied in 47 non-related patients with sickle-cell anemia from the state of Rio Grande do Norte, Brazil. Molecular analysis was conducted by PCR/RFLP using restriction endonucleases XmnI, HindIII, HincII and HinfI to analyze six polymorphic sites from the beta cluster. Twenty-seven patients (57.5%) were identified with genotype CAR/CAR, 9 (19.1%) CAR/BEN, 6 (12.8%) CAR/CAM, 1 (2.1%) BEN/BEN, 2 (4.3%) CAR/Atp, 1 (2.1%) BEN/Atp and 1 (2.1%) with genotype Atp/Atp. The greater frequency of Cameroon haplotypes compared to other Brazilian states suggests the existence of a peculiarity of African origin in the state of Rio Grande do Norte.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
authors.forename: Cynthia Hatsue Kitayama
authors.initials: CH
authors.lastname: Cabral
authors.forename: Edvis Santos Soares
authors.initials: ES
authors.lastname: Serafim
authors.forename: Waleska Rayane Dantas Bezerra
authors.initials: WR
authors.lastname: de Medeiros
authors.forename: Thales Allyrio Araújo
authors.initials: TA
authors.lastname: de Medeiros Fernandes
authors.forename: Elza Miyuki
authors.initials: EM
authors.lastname: Kimura
authors.forename: Fernando Ferreira
authors.initials: FF
authors.lastname: Costa
authors.forename: Maria
authors.initials: M
authors.lastname: de Fátima Sonati
authors.forename: Ivanise Marina Moretti
authors.initials: IM
authors.lastname: Rebecchi
authors.forename: Tereza Maria Dantas
authors.initials: TM
authors.lastname: de Medeiros