title: Dual regulation of breast tubulogenesis using extracellular matrix composition and stromal cells.
abstract: Epithelial-mesenchymal interactions during embryogenesis are critical in defining the phenotype of tissues and organs. The initial elongation of the mammary bud represents a central morphological event requiring extensive epithelial-mesenchymal crosstalk. The precise mechanism orchestrating this outgrowth is still unknown and mostly animal models have been relied upon to explore this process. Highly tunable three-dimensional (3D) culture models are a complementary approach to address the question of phenotypic determination. Here, we used a 3D in vitro culture to study the roles of stromal cells and extracellular matrix components during mammary tubulogenesis. Fibroblasts, adipocytes, and type I collagen actively participated in this process, whereas reconstituted basement membrane inhibited tubulogenesis by affecting collagen organization. We conclude that biochemical and biomechanical signals mediate the interaction between cells and matrix components and are necessary to induce tubulogenesis in vitro.
authors.affiliation: Department of Anatomy and Cellular Biology, School of Medicine, Tufts University, Boston, MA 02111, USA.
authors.forename: Silva
authors.initials: S
authors.lastname: Krause
authors.forename: Adeline
authors.initials: A
authors.lastname: Jondeau-Cabaton
authors.forename: Eugen
authors.initials: E
authors.lastname: Dhimolea
authors.forename: Ana M
authors.initials: AM
authors.lastname: Soto
authors.forename: Carlos
authors.initials: C
authors.lastname: Sonnenschein
authors.forename: Maricel V
authors.initials: MV
authors.lastname: Maffini
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D001485:Basement Membrane / Q000737:chemistry / Q000378:metabolism; D024042:Collagen Type I / Q000096:biosynthesis; D058750:Epithelial-Mesenchymal Transition; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D024042:Collagen Type I