title: A simple method for culture and stable maintenance of giant spheroplasts from the yeast Saccharomyces cerevisiae.
abstract: When spheroplasts of the yeast Saccharomyces cerevisiae are cultured in liquid medium containing osmotic stabilizer, they undergo nuclear division and growth without cell division, resulting in the formation of giant spheroplasts with multinuclei. In this study, we report a simple method for the culture and stable maintenance of giant spheroplasts. The selection of culture media and cell concentration was found to be important for the growth and maintenance of giant spheroplasts. Among the conditions that we tested, static culture in a synthetic Burkholder's medium in 96-well U-bottomed culture plates was most effective. Under appropriate conditions, we could maintain giant spheroplasts for more than 6 days without proliferation of whole cells or marked lysis. The average diameter of spheroplasts can vary from 16 to 53µm, depending on their initial concentration.
authors.affiliation: Department of Environmental Science and Engineering, Graduate School of Science and Engineering,Yamaguchi University, Japan.
authors.forename: Taihei
authors.initials: T
authors.lastname: Fukudomi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kotani
authors.forename: Isamu
authors.initials: I
authors.lastname: Miyakawa
mesh_terms: D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry*; D003847:Deoxyglucose / Q000494:pharmacology*; D009172:Mycology / Q000379:methods*; D012440:Saccharomyces / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D013104:Spheroplasts / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*
chemical_list: D003470:Culture Media; D003847:Deoxyglucose