title: Influence of blocking of 2,3-butanediol pathway on glycerol metabolism for 1,3-propanediol production by Klebsiella oxytoca.
abstract: Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water solubility with 1,3-PD, not only contests the carbon flow and NADH with 1,3-PD but also serves as an obstacle for obtaining high purity 1,3-PD in downstream processes. In this study, a 2,3-BD pathway-deficient mutant of Klebsiella oxytoca ZG36 was constructed by knocking out the budA gene of the wild-type strain M5al. The results of fed-batch fermentation by ZG36 indicated that the glycerol flux and the distribution of metabolites were altered in the K. oxytoca when the 2,3-BD pathway was blocked. No 2,3-BD was produced, and the activity of α-acetolactate decarboxylase (α-ALDC) can not be detected in the fermentation processes. The indexes of the 1,3-PD titer, the conversion from glycerol to 1,3-PD, and the productivity per cell dry weight (CDW) increased by 42%, 62%, and 46%, respectively, compared with the M5al, and the yield of the byproducts also increased obviously. The assay of the enzyme activities in the oxidative branch and the reductive branch of the glycerol metabolism, as well as the intracellular redox state, exposited the results logically.
authors.affiliation: State Key Laboratory for Agrobiotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Guo
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Jilun
authors.initials: J
authors.lastname: Li
mesh_terms: D061249:Batch Cell Culture Techniques; D002072:Butylene Glycols / Q000378:metabolism*; D005285:Fermentation; D005798:Genes, Bacterial / Q000235:genetics; D005990:Glycerol / Q000378:metabolism*; D041121:Klebsiella oxytoca / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D055432:Metabolomics; D009154:Mutation / Q000235:genetics; D009711:Nucleotides / Q000378:metabolism; D010084:Oxidation-Reduction; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011409:Propylene Glycols / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002072:Butylene Glycols; D009711:Nucleotides; D011409:Propylene Glycols; C026978:2,3-butylene glycol; C041787:1,3-propanediol; D005990:Glycerol