title: Targeted mutagenesis of zebrafish: use of zinc finger nucleases.
abstract: The modeling of human disease in the zebrafish (Danio rerio) is moving away from chemical mutagensis and transient downregulation using morpholino oligomers to more targeted and stable transgenic methods. In this respect, zinc finger nucleases offer a means of introducing mutations at targeted sites at high efficiency. We describe here the development of zinc finger nucleases and their general use in model systems with a focus on the zebrafish.
authors.affiliation: Molecular, Cellular and Developmental Biology Research Section, School of Biological Sciences, University of Auckland, Private Bag, Auckland, New Zealand.
authors.forename: Ivone Un San
authors.initials: IU
authors.lastname: Leong
authors.forename: Daniel
authors.initials: D
authors.lastname: Lai
authors.forename: Chuan-Ching
authors.initials: CC
authors.lastname: Lan
authors.forename: Ross
authors.initials: R
authors.lastname: Johnson
authors.forename: Donald R
authors.initials: DR
authors.lastname: Love
authors.forename: Ross
authors.initials: R
authors.lastname: Johnson
authors.forename: Donald R
authors.initials: DR
authors.lastname: Love
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003851:Deoxyribonucleases / Q000378:metabolism*; D018390:Gene Targeting; D006801:Humans; D016296:Mutagenesis; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D003851:Deoxyribonucleases