title: A rapid fluorescence assay for hSMUG1 activity based on modified molecular beacon.
abstract: A new method for assay of hSMUG1 in real-time using molecular beacon is reported. hSMUG1 could be detected linearly in the range from 0.67 nM to 10.05 nM and the detection limit is 0.168 nM. In addition, this method was applied to detect the activity of hSMUG1 in tumor cells and study kinetics. The probe with low background signal has been shown to be suitable for the real-time monitoring of hSMUG1 activity, making this a promising method of high-throughput clinical sample analysis.
authors.affiliation: College of Biology, Hunan University, Changsha, Hunan 410082, People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.forename: Chunyi
authors.initials: C
authors.lastname: Tong
authors.forename: Ying
authors.initials: Y
authors.lastname: Long
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D004260:DNA Repair / Q000235:genetics; D005453:Fluorescence; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007700:Kinetics; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D015335:Molecular Probes / Q000032:analysis / Q000378:metabolism; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence; D051981:Uracil-DNA Glycosidase / Q000032:analysis*
chemical_list: D015335:Molecular Probes; C498095:SMUG1 protein, human; D051981:Uracil-DNA Glycosidase