title: Multifaceted suppression of aggressive behavior of thyroid carcinoma by all-trans retinoic acid induced re-differentiation.
abstract: Since all-trans retinoic acid (ATRA) has shown promising results in differentiation therapy, the present study was designed to investigate the effects of ATRA on thyroid carcinoma and to evaluate the effectiveness of ATRA in redifferentiation induction of thyroid carcinoma. Therefore, we investigated cell growth rate, morphological and nuclear: cytoplasmic ratio, adherent-dependent growth, response to chemotherapy drug following differentiation, T3 and T4 measurement, and critical genes expression pattern. Papillary cell line showed more growth inhibition by ATRA, in addition, mesenchymal and spindle-shape of 8305C cells changed to polygonal. Additionally, high nuclear: cytoplasmic ratio of anaplastic decreased significantly. Redifferentiation significantly suppressed the anchorage-dependent growth in the both cell lines in a dose-dependent manner, potentiated the arsenic trioxide (ATO) effects in anaplastic and papillary cell lines. Furthermore, reduction in the expression of stemness, and invasion related genes was observed in the both cell lines. Altogether, ATRA treatment could hold the aggressive behavior of thyroid carcinoma in restraint and/or potentiate the effect of chemotherapy drug ATO.
authors.affiliation: Endocrinology and Metabolism Research Center (EMRC), Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Malehmir
authors.forename: Vahid
authors.initials: V
authors.lastname: Haghpanah
authors.forename: Bagher
authors.initials: B
authors.lastname: Larijani
authors.forename: Shahin
authors.initials: S
authors.lastname: Ahmadian
authors.forename: Kamran
authors.initials: K
authors.lastname: Alimoghaddam
authors.forename: Ramin
authors.initials: R
authors.lastname: Heshmat
authors.forename: Ardeshir
authors.initials: A
authors.lastname: Ghavamzadeh
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Adabi
authors.forename: Seyed H
authors.initials: SH
authors.lastname: Ghaffari
mesh_terms: D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002277:Carcinoma; D054337:Cell Dedifferentiation; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor / Q000187:drug effects; D053145:Cell Nucleus Size; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D048429:Cell Size; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D013964:Thyroid Neoplasms; D013974:Thyroxine / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D000970:Antineoplastic Agents; D014284:Triiodothyronine; D014212:Tretinoin; D013974:Thyroxine