title: [DNA methylation of microRNA-34b/c and microRNA-124a in gastric cancer].
abstract: OBJECTIVE
  To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma.
  
  
  METHODS
  A total of 80 subjects were divided into gastric carcinoma group (n = 40) and control group (n = 40). The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues.
  
  
  RESULTS
  The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5% (31/40) and 5.0% (2/40) in gastric carcinoma and control groups respectively. There was statistically significant difference between two groups (P < 0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0% (24/40) and 0 in these two groups respectively. There was statistically significant difference between two groups (P < 0.05). Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.
  
  
  CONCLUSIONS
  The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.
authors.affiliation: Department of Gastroenterology, Tianjin Medical University Hospital, Tianjin 300052, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Hong-yu
authors.initials: HY
authors.lastname: Yue
authors.forename: Wen-tian
authors.initials: WT
authors.lastname: Liu
authors.forename: Wei-li
authors.initials: WL
authors.lastname: Fang
mesh_terms: D019175:DNA Methylation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011401:Promoter Regions, Genetic; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: C509207:MIRN124 microRNA, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs