title: LUMP is a putative double-stranded RNA binding protein required for male fertility in Drosophila melanogaster.
abstract: In animals, male fertility requires the successful development of motile sperm. During Drosophila melanogaster spermatogenesis, 64 interconnected spermatids descended from a single germline stem cell are resolved into motile sperm in a process termed individualization. Here we identify a putative double-stranded RNA binding protein LUMP that is required for male fertility. lump(1) mutants are male-sterile and lack motile sperm due to defects in sperm individualization. We show that one dsRNA binding domains (dsRBD) is essential for LUMP function in male fertility. These findings reveal LUMP is a novel factor required for late stages of male germline differentiation.
authors.affiliation: Department of Pharmacology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
authors.forename: Charcacia
authors.initials: C
authors.lastname: Sanders
authors.forename: Dean P
authors.initials: DP
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005298:Fertility / Q000235:genetics; D005786:Gene Expression Regulation; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012330:RNA, Double-Stranded / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013087:Spermatids / Q000166:cytology / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D013094:Spermatozoa / Q000254:growth & development / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D035683:MicroRNAs; C478068:R2D2 protein, Drosophila; D012330:RNA, Double-Stranded; D016601:RNA-Binding Proteins; D011494:Protein Kinases; C094331:Ste protein, Drosophila