title: Conformational dynamics of single G protein-coupled receptors in solution.
abstract: G protein-coupled receptors (GPCRs) comprise a large family of seven-helix transmembrane proteins which regulate cellular signaling by sensing light, ligands, and binding proteins. The GPCR activation process, however, is not a simple on-off switch; current models suggest a complex conformational landscape in which the active, signaling state includes multiple conformations with similar downstream activity. The present study probes the conformational dynamics of single β(2)-adrenergic receptors (β(2)ARs) in the solution phase by Anti-Brownian ELectrokinetic (ABEL) trapping. The ABEL trap uses fast electrokinetic feedback in a microfluidic configuration to allow direct observation of a single fluorescently labeled β(2)AR for hundreds of milliseconds to seconds. By choosing a reporter dye and labeling site sensitive to ligand binding, we observe a diversity of discrete fluorescence intensity and lifetime levels in single β(2)ARs, indicating a varying radiative lifetime and a range of discrete conformational states with dwell times of hundreds of milliseconds. We find that the binding of agonist increases the dwell times of these states, and furthermore, we observe millisecond fluctuations within states. The intensity autocorrelations of these faster fluctuations are well-described by stretched exponential functions with a stretching exponent β ~ 0.5, suggesting protein dynamics over a range of time scales.
authors.affiliation: Department of Chemistry, Stanford University, Stanford, California 94305, United States.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bockenhauer
authors.forename: Alexandre
authors.initials: A
authors.lastname: Fürstenberg
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Yao
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kobilka
authors.forename: W E
authors.initials: WE
authors.lastname: Moerner
mesh_terms: D008024:Ligands; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D011789:Quantum Theory; D018343:Receptors, Adrenergic, beta-2 / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry*; D013997:Time Factors
chemical_list: D008024:Ligands; D018343:Receptors, Adrenergic, beta-2; D012996:Solutions