title: Comparative study of lipid extraction from microalgae by organic solvent and supercritical CO2.
abstract: Pavlova sp. was employed to evaluate the efficiency of different lipid extraction methods. The microalgal crude lipids content determined using the mixed solvent with ultrasonic method was 44.7 wt.%. The triglyceride content obtained by the mixed solvent method was 15.6 wt.%. The extraction yield was the FAME yield divided by the maximum FAME (15.9 wt.%). The extraction yield was improved by cell disruption prior to extraction, and the highest triglyceride extraction yield of 98.7% was observed using the supercritical fluid extraction (SFE) method with bead-beating. The results indicate that the SFE method is effective and provides higher selectivity for triglyceride extraction though the total lipid extracted was less than that using solvent extraction.
authors.affiliation: Green Energy and Environment Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan, ROC. chenhsicheng@itri.org.tw
authors.forename: Chen-Hsi
authors.initials: CH
authors.lastname: Cheng
authors.forename: Tz-Bang
authors.initials: TB
authors.lastname: Du
authors.forename: Hsien-Chueh
authors.initials: HC
authors.lastname: Pi
authors.forename: Shyue-Ming
authors.initials: SM
authors.lastname: Jang
authors.forename: Yun-Huin
authors.initials: YH
authors.lastname: Lin
authors.forename: Hom-Ti
authors.initials: HT
authors.lastname: Lee
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D045424:Complex Mixtures; D004952:Esters / Q000032:analysis; D058087:Haptophyta / Q000378:metabolism*; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D045424:Complex Mixtures; D004952:Esters; D008055:Lipids; D009930:Organic Chemicals; D012997:Solvents; D002245:Carbon Dioxide