title: Y-autosome translocation interferes with meiotic sex inactivation and expression of autosomal genes: a case study in the pig.
abstract: Y-autosome translocations are rare in humans and pigs. In both species, these rearrangements can be responsible for meiotic arrest and subsequent infertility. Chromosome pairing abnormalities on the SSCX, SSCY and SSC1 chromatin domains were identified by analyzing pachytene spermatocytes from a boar carrying a (Y;1) translocation by immunolocalization of specific meiotic protein combined with FISH. Disturbance of the meiotic sex chromosome inactivation (MSCI) was observed by Cot-RNA-FISH and analysis of ZFY gene expression by sequential RNA- and DNA-FISH on spermatocytes. We hypothesized that the meiotic arrest observed in this boar might be due to the silencing of critical autosomal genes and/or the reactivation of some sex chromosome genes.
authors.affiliation: Laboratoire de Génétique Cellulaire, UMR 444, Institut National de la Recherche Agronomique, Université de Toulouse, Toulouse, France.
authors.forename: H
authors.initials: H
authors.lastname: Barasc
authors.forename: N
authors.initials: N
authors.lastname: Mary
authors.forename: R
authors.initials: R
authors.lastname: Letron
authors.forename: A
authors.initials: A
authors.lastname: Calgaro
authors.forename: A M
authors.initials: AM
authors.lastname: Dudez
authors.forename: N
authors.initials: N
authors.lastname: Bonnet
authors.forename: Y
authors.initials: Y
authors.lastname: Lahbib-Mansais
authors.forename: M
authors.initials: M
authors.lastname: Yerle
authors.forename: A
authors.initials: A
authors.lastname: Ducos
authors.forename: A
authors.initials: A
authors.lastname: Pinton
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D020868:Gene Silencing; D017404:In Situ Hybridization, Fluorescence; D059785:Karyotype; D051741:Kruppel-Like Transcription Factors / Q000235:genetics; D008297:Male; D008540:Meiosis; D012313:RNA / Q000032:analysis; D013090:Spermatocytes / Q000737:chemistry; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000473:pathology; D013737:Testis / Q000473:pathology; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D014960:X Chromosome / Q000235:genetics*; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D051741:Kruppel-Like Transcription Factors; C054430:ZFY protein, human; D012313:RNA; D004247:DNA