title: Characterization of the secretome of chickpea suspension culture reveals pathway abundance and the expected and unexpected secreted proteins.
abstract: The secretome of an organism is defined as a set of secreted proteins that encompasses all proteins exported to the extracellular space. To better understand the chickpea secretome, we used callus culture to isolate and identify secreted proteins as a step toward determining their functions. Proteins in the extracellular media of the suspension culture were examined using SDS-PAGE and mass spectrometry (LC-MS/MS). Proteomic analysis led to the identification of 773 proteins, presumably involved in a variety of functions including metabolism, signal transduction, transport, and cell defense, in addition to maintaining redox status of extracellular space. Bioinformatic analysis confirmed 724 proteins, accounting for 94% of the identified proteins, as constituents of the secretome. Analysis of the secretome revealed the presence of several proteins of unknown function and a large number of classical and nonclassical secreted proteins. This represents the first comprehensive secretome of a legume genome, which is yet to be sequenced. Comparative analysis of the chickpea secretome with those of Medicago, Arabidopsis, and rice revealed that the majority of identified proteins are seemingly species-specific. This study demonstrates that characterization of the chickpea secretome in vitro can be used to identify secreted proteins, which has implications for systems biology research.
authors.affiliation: National Institute of Plant Genome Research , Aruna Asaf Ali Marg, New Delhi-110067, India.
authors.forename: Sonika
authors.initials: S
authors.lastname: Gupta
authors.forename: Vijay
authors.initials: V
authors.lastname: Wardhan
authors.forename: Shikha
authors.initials: S
authors.lastname: Verma
authors.forename: Saurabh
authors.initials: S
authors.lastname: Gayali
authors.forename: Uma
authors.initials: U
authors.lastname: Rajamani
authors.forename: Asis
authors.initials: A
authors.lastname: Datta
authors.forename: Subhra
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Niranjan
authors.initials: N
authors.lastname: Chakraborty
mesh_terms: D029863:Cicer / Q000378:metabolism*; D046508:Culture Techniques; D053858:Metabolic Networks and Pathways; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D021382:Protein Sorting Signals; D059748:Proteolysis; D020543:Proteome / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D010940:Plant Proteins; D021382:Protein Sorting Signals; D020543:Proteome