title: [Antagonism of tert-butylhydroquinone on neurotoxicity and oxidative stress induced by paraquat in PC12].
abstract: OBJECTIVE
  To investigate the protective effects of the tert-butylhydroquinone (tBHQ) pretreatment on neurotoxicity and oxidative stress induced by paraquat (PQ) in PC12 cells.
  
  
  METHODS
  Cytotoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with different concentrations of 100, 300 micromol/L PQ for 24 h and 48 h. PC12 cells were pretreated with or without 40 micromol/L tBHQ for 4 h, PC12 cells were exposed to PQ at the doses of 0, 100, 300 micromol/L for 24 h and 48 h, respectively. The viability of PC12 cells was measured by MTT assay, the apoptosis rates of PC12 cells were detected by flow cytometry (FCM) and the malondialdehyde (MDA) levels of PC12 cells were examine by thiobarbituric acid (TBA) method.
  
  
  RESULTS
  When the exposure doses of PQ were 100 and 300 micromol/L for 24 h, the viability of PC12 cells pretreated with tBHQ was significantly higher than that of PC12 cells only exposed to PQ (P < 0.05 or P < 0.01). When the exposure dose of PQ was 100 micromol/L for 48 h, the viability of PC12 cells pretreated with tBHQ was significantly higher than that of PC12 cells only exposed to PQ (P < 0.01). When the exposure doses of PQ were 100 and 300 micromol/L for 24 h, the apoptosis rates and MDA levels of PC12 cells pretreated with tBHQ were significantly lower than those of PC12 cells only exposed to PQ (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  tBHQ pretreatment can reduce the cytotoxicity, apoptosis and oxidative stress induced by PQ in PC12 cells.
authors.affiliation: Department of Occupational and Environmental Health, Research Unit for Toxicology and Chemical Safety Assessment, Institution of Environmental and Health, Major Subject of Environment and Health of Fujian Key Universities, School of Public Health, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Huang-Yuan
authors.initials: HY
authors.lastname: Li
authors.forename: Si-Ying
authors.initials: SY
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Zhang-Jing
authors.initials: ZJ
authors.lastname: Wang
authors.forename: Peng-Ling
authors.initials: PL
authors.lastname: Ye
authors.forename: Qiu-Feng
authors.initials: QF
authors.lastname: Han
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D006873:Hydroquinones / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D010269:Paraquat / Q000633:toxicity*; D051381:Rats; D017382:Reactive Oxygen Species / Q000032:analysis
chemical_list: D006873:Hydroquinones; D017382:Reactive Oxygen Species; C018855:2-tert-butylhydroquinone; D010269:Paraquat