title: Effects of ANGPTL3 antisense oligodeoxynucleotides transfection on the cell growths and invasion of human hepatocellular carcinoma cells.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of antisense oligodeoxynucleotides transfection of angiopioetin-like protein 3 (ANGPTL3) on the proliferation and invasion capacities of human hepatocellular carcinoma SMMC-7721 cells in vitro.
  
  
  METHODS
  ANGPTL3 antisense oligodeoxynucleotides was transfected into SMMC-7721 cells with Lipofectamine 2000. We used MTT assay to evaluate cell growth. ANGPTL3, p38 mitogen-activated protein kinases (MAPKs) and Matrix metalloproteinase-9 (MMP-9) mRNA expression levels were evaluated by RT-PCR. The invasive potency of SMMC-7721 cells was measured by the transwell cell invasion assay.
  
  
  RESULTS
  ANGPTL3 antisense oligodeoxynucleotides were successfully transfected into SMMC-7721 cells, resulting in the significant inhibition of ANGPTL3, p38MAPK and MMP-9 mRNA expression. Downregulation of ANGPTL3 inhibited cell proliferation and decreased invasion of SMMC-7721 cells.
  
  
  CONCLUSIONS
  ANGPTL3 may be functionally involved in hepatocellular carcinoma cell proliferation and invasion and is a potential target for hepatocellular carcinoma therapy.
authors.affiliation: Department of Hepatobiliary Surgery, Henan Provincial People's Hospital, Zhengzhou, China. yhb2101661@sina.com
authors.forename: Haibo
authors.initials: H
authors.lastname: Yu
authors.forename: Huifeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Deyu
authors.initials: D
authors.lastname: Li
authors.forename: Huanzhou
authors.initials: H
authors.lastname: Xue
authors.forename: Chengen
authors.initials: C
authors.lastname: Pan
authors.forename: Sihai
authors.initials: S
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000091303:Angiopoietin-Like Protein 3; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000473:pathology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C479896:ANGPTL3 protein, human; D000091303:Angiopoietin-Like Protein 3; D000075802:Angiopoietin-like Proteins; D042682:Angiopoietins; D009838:Oligodeoxyribonucleotides; D048051:p38 Mitogen-Activated Protein Kinases; D020780:Matrix Metalloproteinase 9