title: [Methylation of insulin-like growth factor binding protein 3 gene in neonates with intrauterine growth restriction].
abstract: OBJECTIVE
  To study the role of promoter methylation of insulin-like growth factor binding protein 3 (IGFBP3) in intrauterine growth restriction (IUGR).
  
  
  METHODS
  Fifty neonates with IUGR and 30 healthy neonates were enrolled. The promoter methylation status of IGFBP3 in peripheral blood was evaluated by methylation-specific PCR (MSP) and high resolution melting (HRM) techniques.
  
  
  RESULTS
  The complete methylation rate, partial methylation rate and non-methylation rate of IGFBP3 promoter in the IUGR group was 4% (2/50), 40% (20/50) and 56% (28/50), respectively. The partial methylation rate and non-methylation rate of IGFBP3 promoter in the control group were 13% (4/30) and 87% (26/30), respectively. There were significant differences in the promoter methylation rate of IGFBP3 between the two groups (P<0.01).
  
  
  CONCLUSIONS
  The promoter methylation of IGFBP3 gene is associated with the pathogenesis of IUGR.
authors.affiliation: Department of Pediatrics, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, China.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Su
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Qin-Yu
authors.initials: QY
authors.lastname: Ge
mesh_terms: D019175:DNA Methylation; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology* / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics*; D008297:Male; D011401:Promoter Regions, Genetic
chemical_list: C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3