title: Manipulating location, polarity, and outgrowth length of neuron-like pheochromocytoma (PC-12) cells on patterned organic electrode arrays.
abstract: In this manuscript, we describe a biocompatible organic electrode system, comprising poly(3,4-ethylenedioxythiophene) (PEDOT) microelectrode arrays on indium tin oxide (ITO) glass, that can be used to regulate the neuron type, location, polarity, and outgrown length of neuron-like cells (PC-12). We fabricated a PEDOT microelectrode array with four different sizes (flat; 20, 50, and 100 μm) through electrochemical polymerization. Extracellular matrix proteins absorbed well on these organic electrodes; cells absorbed selectively on the organic electrodes when we used polyethylene oxide/polypropylene oxide/polyethylene oxide triblock copolymers (PEO/PPO/PEO, Pluronic™ F108) as the anti-adhesive coating. In this system, the neurite polarities and neuron types could be manipulated by varying the width of the PEDOT microelectrode arrays. On the unpatterned PEDOT electrode, PC-12 cells were randomly polarized, with approximately 80% having multi-polar cell types. In contrast, when we cultured PC-12 cells on the 20 μm wide PEDOT line array, the neurites aligned along the direction of the organic electrodes, with the percentage of uni- and bipolar PC-12 cells increasing to greater than 90%. The outgrowth of neurites on the microelectrodes was promoted by ~60% with an applied electrical stimulation. Therefore, these electroactive PEDOT microelectrode arrays have potential for use in tissue engineering related to the development and regeneration of mammalian nervous systems.
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan 11529.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Hsiao
authors.forename: Chung-Chih
authors.initials: CC
authors.lastname: Lin
authors.forename: Hsin-Jui
authors.initials: HJ
authors.lastname: Hsieh
authors.forename: Shih-Min
authors.initials: SM
authors.lastname: Tsai
authors.forename: Chiung-Wen
authors.initials: CW
authors.lastname: Kuo
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Chu
authors.forename: Peilin
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004558:Electric Stimulation; D046228:Microarray Analysis; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology / Q000502:physiology; D016716:PC12 Cells; D010673:Pheochromocytoma; D020442:Poloxamer / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017971:Tin Compounds / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; D017971:Tin Compounds; C121383:poly(3,4-ethylene dioxythiophene); D020442:Poloxamer; D011092:Polyethylene Glycols; C109984:indium tin oxide