title: Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori.
abstract: Epigenetic information is encoded in post-translational modifications (PTMs) of histones. Various combinations of these marks contribute to the regulation of chromatin-templated DNA metabolisms. The histone code is gradually translated into biological responses in model organisms. However, in the silkworm, the modifications of histones with unique holocentric chromosomes have not yet been analyzed. TAU-PAGE analysis of the silkworm histone variants H2A, H2B, and H3, separated by RP-HPLC, suggested silkworm specific modification. Detailed mass spectrometry analyses of the peptides derived from the N-terminus of the silkworm H3.2 generated by glutamyl endopeptidase, lysyl endopeptidase, and trypsin digestions revealed global modifications around H3K9.
authors.affiliation: Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mon
authors.forename: Makiko
authors.initials: M
authors.lastname: Izumi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Mitsunobu
authors.forename: Tsuneyuki
authors.initials: T
authors.lastname: Tatsuke
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iiyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Jikuya
authors.forename: Jae Man
authors.initials: JM
authors.lastname: Lee
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kusakabe
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D011499:Protein Processing, Post-Translational; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D006657:Histones