title: Quantitative proteomic analysis of the adipocyte plasma membrane.
abstract: The adipocyte is a key regulator of mammalian metabolism. To advance our understanding of this important cell, we have used quantitative proteomics to define the protein composition of the adipocyte plasma membrane (PM) in the presence and absence of insulin. Using this approach, we have identified a high confidence list of 486 PM proteins, 52 of which potentially represent novel cell surface proteins, including a member of the adiponectin receptor family and an unusually high number of hydrolases with no known function. Several novel insulin-responsive proteins including the sodium/hydrogen exchanger, NHE6 and the collagens III and VI were also identified, and we provide evidence of PM-ER association suggestive of a unique functional association between these two organelles in the adipocyte. Together these studies provide a wealth of potential therapeutic targets for the manipulation of adipocyte function and a valuable resource for metabolic research and PM biology.
authors.affiliation: Diabetes and Obesity Program,  Garvan Institute of Medical Research , Sydney, NSW 2010, Australia.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Prior
authors.forename: Mark
authors.initials: M
authors.lastname: Larance
authors.forename: Robert T
authors.initials: RT
authors.lastname: Lawrence
authors.forename: Jamie
authors.initials: J
authors.lastname: Soul
authors.forename: Sean
authors.initials: S
authors.lastname: Humphrey
authors.forename: James
authors.initials: J
authors.lastname: Burchfield
authors.forename: Carol
authors.initials: C
authors.lastname: Kistler
authors.forename: Jonathon R
authors.initials: JR
authors.lastname: Davey
authors.forename: Penelope J
authors.initials: PJ
authors.lastname: La-Borde
authors.forename: Michael
authors.initials: M
authors.lastname: Buckley
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanazawa
authors.forename: Robert G
authors.initials: RG
authors.lastname: Parton
authors.forename: Michael
authors.initials: M
authors.lastname: Guilhaus
authors.forename: David E
authors.initials: DE
authors.lastname: James
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D037281:Calnexin / Q000302:isolation & purification / Q000378:metabolism; D051242:Caveolin 1 / Q000302:isolation & purification / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D040901:Proteomics; D050765:Qa-SNARE Proteins / Q000302:isolation & purification / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D051438:Syntaxin 16 / Q000302:isolation & purification / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D051242:Caveolin 1; D008565:Membrane Proteins; C503961:NHE6 protein, mouse; D050765:Qa-SNARE Proteins; D017923:Sodium-Hydrogen Exchangers; D051438:Syntaxin 16; D037281:Calnexin