title: Assessment of ¹⁸⁸Re marked anti MHC class II antibody by peripheral blood mononuclear cells stimulated by donor alloantigen.
abstract: BACKGROUND
  Previous studies showed that anti MHC-II monoclone antibody (MAb) only had partial inhibiting effect of alloreactive mixed lymphocyte reaction (MLR) in vitro and it was unsteady and non-persistent. The aim of this research was to determine whether radioactive isotope (188)Re marked MHC-II antibody could benefit the allograft acceptance in transplantation as compared to normal MHC-II antibody.
  
  
  METHODS
  188Re was incorporated to 2E9/13F (ab')(2) which is against swine MHC class II antigen (MAb-(188)Re). Porcine peripheral blood mononuclear (PBMC) cells were examined for proliferation and cytokine mRNA expression after stimulation with MHC-II MAb or MAb-(188)Re.
  
  
  RESULTS
  The proliferative response of recipient PBMCs in mixed lymphocyte reaction (MLR) to donor alloantigen showed that the stimulation index of MAb-(188)Re group was significantly lower than the MHC-II MAb group and control (P < 0.05). mRNA expression of interleukin 2, interferon Υ and tumor necrosis factor α (type 1 cytokines) was lower in MAb-(188)Re group than the MHC-II MAb group, while interleukin 10 (type 2 cytokines) was higher in MAb-(188)Re group in the first 24 hours.
  
  
  CONCLUSIONS
  MAb-(188)Re could help the graft acceptance by inhibiting T cell proliferation, lowering the expression of type 1 cytokines and elevating the type 2 cytokines produced by PBMC.
authors.affiliation: Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Ding
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Cao
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Da-Ren
authors.initials: DR
authors.lastname: Liu
authors.forename: Ri-Sheng
authors.initials: RS
authors.lastname: Que
authors.forename: Lin-Hua
authors.initials: LH
authors.lastname: Zhu
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhou
authors.forename: Ke-Jie
authors.initials: KJ
authors.lastname: Mao
authors.forename: Jun-An
authors.initials: JA
authors.lastname: Hu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D016753:Interleukin-10 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D007519:Isoantigens / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000528:radiation effects; D007959:Lymphocyte Culture Test, Mixed; D016685:Mitomycin / Q000494:pharmacology; D011868:Radioisotopes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012211:Rhenium; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007519:Isoantigens; D011868:Radioisotopes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D016685:Mitomycin; D012211:Rhenium