title: Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor.
abstract: The effects of inoculum density, aeration volume and culture period on accumulation of biomass and secondary metabolites in adventitious roots of Hypericum perforatum in balloon type airlift bioreactors (3 l capacity) were investigated. The greatest increment of biomass as well as metabolite content occurred at an inoculum density of 3 g l(-1) and an aeration volume of 0.1 vvm. After 6 weeks of culture, an approximately 50-fold increase in biomass was recorded containing 60.11 mg g(-1) dry weight (DW) of phenolics, 42.7 mg g(-1) DW of flavonoids and 0.80 mg g(-1) DW of chlorogenic acid. Liquid chromatography-mass spectroscopy/mass spectroscopy demonstrated that the presence of quercetin and hyperoside in adventitious roots at a level of 1.33 and 14.01 μg g(-1) DW, respectively after 6 weeks of culture. The results suggest scale-up of adventitious root culture of H. perforatum for the production of chlorogenic acid, quercetin and hyperoside is feasible.
authors.affiliation: Research Center for Development of Advanced Horticultural Technology, Chungbuk National University, Chungju 361-763, Republic of Korea.
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Cui
authors.forename: Hosakatte Niranjana
authors.initials: HN
authors.lastname: Murthy
authors.forename: You-Xun
authors.initials: YX
authors.lastname: Jin
authors.forename: Yong-Hyeon
authors.initials: YH
authors.lastname: Yim
authors.forename: Ji-Yeong
authors.initials: JY
authors.lastname: Kim
authors.forename: Kee-Yoeup
authors.initials: KY
authors.lastname: Paek
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D001713:Biphenyl Compounds / Q000378:metabolism; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020902:Hypericum / Q000254:growth & development* / Q000378:metabolism*; D007700:Kinetics; D008315:Malondialdehyde / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010851:Picrates / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D008315:Malondialdehyde; C021304:hyperoside; D011794:Quercetin; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl