title: Single intravenous and oral dose pharmacokinetics of florfenicol in the channel catfish (Ictalurus punctatus).
abstract: Plasma distribution and elimination of florfenicol in channel catfish were investigated after a single dose (10 mg/kg) of intravenous (i.v.) or oral administration in freshwater at a mean water temperature of 25.4 °C. Florfenicol concentrations in plasma were analyzed by means of liquid chromatography with MS/MS detection. After i.v. florfenicol injection, the terminal half-life (t(1/2)), volume of distribution at steady state (V(ss)), and central volume of distribution (V(c)) were 8.25 h, 0.9 and 0.381 L/kg, respectively. After oral administration of florfenicol, the terminal t(1/2), C(max), T(max), and oral bioavailability (F) were 9.11 h, 7.6 μg/mL, 9.2 h, and 1.09, respectively. There was a lag absorption time of 1.67 h in oral dosing. Results from these studies support that 10 mg florfenicol/kg body weight in channel catfish is an efficacious dosage following oral administration.
authors.affiliation: Mississippi State University, College of Veterinary Medicine, Stoneville, MS, USA. gaunt@cvm.msstate.edu
authors.forename: P S
authors.initials: PS
authors.lastname: Gaunt
authors.forename: C
authors.initials: C
authors.lastname: Langston
authors.forename: C
authors.initials: C
authors.lastname: Wrzesinski
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: P
authors.initials: P
authors.lastname: Adams
authors.forename: L
authors.initials: L
authors.lastname: Crouch
authors.forename: D
authors.initials: D
authors.lastname: Sweeney
authors.forename: R
authors.initials: R
authors.lastname: Endris
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D019540:Area Under Curve; D001682:Biological Availability; D002853:Chromatography, Liquid; D006207:Half-Life; D007059:Ictaluridae / Q000097:blood*; D007275:Injections, Intravenous; D053719:Tandem Mass Spectrometry; D013839:Thiamphenicol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
chemical_list: D000900:Anti-Bacterial Agents; C035534:florfenicol; D013839:Thiamphenicol