title: Leucine biosynthesis regulates cytoplasmic iron-sulfur enzyme biogenesis in an Atm1p-independent manner.
abstract: Fe-S clusters (ISCs) are versatile cofactors utilized by many mitochondrial, cytoplasmic, and nuclear enzymes. Whereas mitochondria can independently initiate and complete ISC synthesis, other cellular compartments are believed to assemble ISCs from putative precursors exported from the mitochondria via an ATP binding cassette (ABC) transporter conserved from yeast (Atm1p) to humans (ABCB7). However, the regulatory interactions between mitochondrial and extramitochondrial ISC synthesis are largely unknown. In yeast, we found that mitochondrial ISC synthesis is regulated by the leucine biosynthetic pathway, which among other proteins involves an abundant cytoplasmic [4Fe-4S] enzyme, Leu1p. Enzymatic blocks in the pathway (i.e. leu1Δ or leu2Δ gene deletion mutations) induced post-transcriptional up-regulation of core components of mitochondrial ISC biosynthesis (i.e. the sulfur donor Nfs1p, the iron donor Yfh1p, and the ISC scaffold Isu1p). In leu2Δ cells, transcriptional mechanisms also led to dramatic up-regulation of Leu1p with concomitant down-regulation of mitochondrial aconitase (Aco1p), a [4Fe-4S] enzyme in the tricarboxylic acid cycle. Accordingly, the leu2Δ deletion mutation exacerbated Aco1p inactivation in cells with mutations in Yfh1p. These data indicate that defects in leucine biosynthesis promote the biogenesis of enzymatically active Leu1p at the expense of Aco1p activity. Surprisingly, this effect is independent of Atm1p; previous reports linking the loss of Leu1p activity to Atm1p depletion were confounded by the fact that LEU2 was used as a selectable marker to create Atm1p-depleted cells, whereas a leu2Δ allele was present in Atm1p-repleted controls. Thus, still largely unknown transcriptional and post-transcriptional mechanisms control ISC distribution between mitochondria and other cellular compartments.
authors.affiliation: Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: Tibor
authors.initials: T
authors.lastname: Bedekovics
authors.forename: Hongqiao
authors.initials: H
authors.lastname: Li
authors.forename: Gabriella B
authors.initials: GB
authors.lastname: Gajdos
authors.forename: Grazia
authors.initials: G
authors.lastname: Isaya
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D019069:Cell Respiration; D003593:Cytoplasm / Q000378:metabolism*; D004271:DNA, Fungal / Q000378:metabolism; D015536:Down-Regulation; D035941:Iron Regulatory Protein 1 / Q000096:biosynthesis* / Q000378:metabolism; D007930:Leucine / Q000096:biosynthesis*; D008928:Mitochondria / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: C091561:ATM1 protein, S cerevisiae; D018528:ATP-Binding Cassette Transporters; D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D035941:Iron Regulatory Protein 1; D007930:Leucine