title: DNA methylation detection: bisulfite genomic sequencing analysis.
abstract: DNA methylation, which most commonly occurs at the C5 position of cytosines within CpG dinucleotides, plays a pivotal role in many biological procedures such as gene expression, embryonic development, cellular proliferation, differentiation, and chromosome stability. Aberrant DNA methylation is often associated with loss of DNA homeostasis and genomic instability leading to the development of human diseases such as cancer. The importance of DNA methylation creates an urgent demand for effective methods with high sensitivity and reliability to explore innovative diagnostic and therapeutic strategies. Bisulfite genomic sequencing developed by Frommer and colleagues was recognized as a revolution in DNA methylation analysis based on conversion of genomic DNA by using sodium bisulfite. Besides various merits of the bisulfite genomic sequencing method such as being highly qualitative and quantitative, it serves as a fundamental principle to many derived methods to better interpret the mystery of DNA methylation. Here, we present a protocol currently frequently used in our laboratory that has proven to yield optimal outcomes. We also discuss the potential technical problems and troubleshooting notes for a variety of applications in this field.
authors.affiliation: Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Trygve O
authors.initials: TO
authors.lastname: Tollefsbol
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D019175:DNA Methylation / Q000187:drug effects*; D023281:Genomics / Q000379:methods*; D007753:Laboratories; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D013447:Sulfites; D004247:DNA; C042345:hydrogen sulfite