title: Characterization of the human CD8⁺ T cell response following infection with 2009 pandemic influenza H1N1 virus.
abstract: The 2009 H1N1 influenza pandemic provided an opportunity to study human virus-specific T cell responses after infection with a novel influenza virus against which limited humoral immunity existed in the population. Here we describe the magnitude, kinetics, and nature of the virus-specific T cell response using intracellular gamma interferon (IFN-γ) staining and fluorochrome-labeled major histocompatibility complex (MHC) class I-peptide complexes. We demonstrate that influenza virus-infected patients develop recall T cell responses that peak within 1 week postinfection and that contract rapidly. In particular, effector cell frequencies declined rapidly postinfection in favor of relatively larger proportions of central memory cells.
authors.affiliation: Department of Virology, Erasmus Medical Center, P. O. Box 2040, 3000 CA Rotterdam, The Netherlands.
authors.forename: M L B
authors.initials: ML
authors.lastname: Hillaire
authors.forename: S E
authors.initials: SE
authors.lastname: van Trierum
authors.forename: R
authors.initials: R
authors.lastname: Bodewes
authors.forename: C A
authors.initials: CA
authors.lastname: van Baalen
authors.forename: R S
authors.initials: RS
authors.lastname: van Binnendijk
authors.forename: M P
authors.initials: MP
authors.lastname: Koopmans
authors.forename: R A M
authors.initials: RA
authors.lastname: Fouchier
authors.forename: A D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: G F
authors.initials: GF
authors.lastname: Rimmelzwaan
mesh_terms: D000293:Adolescent; D000328:Adult; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling; D013997:Time Factors; D055815:Young Adult
chemical_list: D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma