title: Chlamydia pneumoniae binds to the lectin-like oxidized LDL receptor for infection of endothelial cells.
abstract: The association of Chlamydia pneumoniae and atherosclerosis has been well documented. Recently, it has been demonstrated that C. pneumoniae up-regulates expression of the lectin-like ox-LDL receptor (LOX-1) in endothelial cells. Many of the pro-atherogenic effects of ox-LDL occur through its activation and uptake by LOX-1. This class E scavenger receptor contains a carbohydrate-recognition domain common to the C type lectin family. Previously, we have demonstrated that the major outer membrane protein of the chlamydiae is glycosylated and glycan removal abrogates infectivity of C. pneumoniae for endothelial cells. In this study, we investigated whether C. pneumoniae binds to LOX-1. The results show that 1) infection of endothelial cells by C. pneumoniae is inhibited by ligands that bind to the LOX-1 receptor, but not by ligands binding to other scavenger receptors; 2) anti-LOX-1 antibody inhibits C. pneumoniae infectivity, while antibodies against other scavenger receptors do not; 3) anti-LOX-1 antibody inhibits attachment of C. pneumoniae to endothelial cells; and 4) C. pneumoniae co-localizes with LOX-1. These effects were not observed for Chlamydia trachomatis. In conclusion, C. pneumoniae binds to the LOX-1 receptor, which is known to promote atherosclerosis.
authors.affiliation: Department of Epidemiology, University of Washington, Seattle, WA 98195, USA.
authors.forename: Lee Ann
authors.initials: LA
authors.lastname: Campbell
authors.forename: Mirja
authors.initials: M
authors.lastname: Puolakkainen
authors.forename: Amy
authors.initials: A
authors.lastname: Lee
authors.forename: Michael E
authors.initials: ME
authors.lastname: Rosenfeld
authors.forename: H Jacques
authors.initials: HJ
authors.lastname: Garrigues
authors.forename: Cho-Chou
authors.initials: CC
authors.lastname: Kuo
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D050197:Atherosclerosis / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000378:metabolism* / Q000472:pathogenicity; D042783:Endothelial Cells / Q000378:metabolism / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism; D051131:Receptors, Oxidized LDL / Q000378:metabolism*; D051127:Scavenger Receptors, Class E / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000911:Antibodies, Monoclonal; D037102:Lectins; C495137:OLR1 protein, human; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E