title: Peptide-displaying phage technology in glycobiology.
abstract: Phage display technology is an emerging drug discovery tool. Using that approach, short peptides that mimic part of a carbohydrate's conformation are selected by screening a peptide-displaying phage library with anti-carbohydrate antibodies. Chemically synthesized peptides with an identified sequence have been used as an alternative ligand to carbohydrate-binding proteins. These peptides represent research tools useful to assay the activities of glycosyltransferases and/or sulfotransferases or to inhibit the carbohydrate-dependent binding of proteins in vitro and in vivo. Peptides can also serve as immunogens to raise anti-carbohydrate antibodies in vivo in animals. Phage display has also been used in single-chain antibody technology by inserting an immunoglobulin's variable region sequence into the phage. A single-chain antibody library can then be screened with a carbohydrate antigen as the target, resulting in a recombinant anti-carbohydrate antibody with high affinity to the antigen. This review provides examples of successful applications of peptide-displaying phage technology to glycobiology. Such an approach should benefit translational research by supplying carbohydrate-mimetic peptides and carbohydrate-binding polypeptides.
authors.affiliation: Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA. michiko@sanfordburnham.org
authors.forename: Michiko N
authors.initials: MN
authors.lastname: Fukuda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry; D040761:Biomimetic Materials; D054794:Glycomics; D006801:Humans; D055767:Immobilized Proteins; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011134:Polysaccharides / Q000276:immunology; D011485:Protein Binding; D057127:Single-Chain Antibodies / Q000302:isolation & purification
chemical_list: D000941:Antigens; D055767:Immobilized Proteins; D019151:Peptide Library; D010455:Peptides; D011134:Polysaccharides; D057127:Single-Chain Antibodies