title: Native chromatin immunoprecipitation.
abstract: Native chromatin immunoprecipitation refers to a method that allows for identification and quantification of DNA that is associated with specific chromatin proteins without altering the structure of these proteins. The method has been used with great success in the past and has some advantages over the more widely used cross-linking chromatin immunoprecipitation. We describe here a protocol that was specifically optimized for low cell numbers.
authors.affiliation: Ecologie et Evolution des Interactions, 2EI, Université de Perpignan, Perpignan Cedex, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Cosseau
authors.forename: Christoph
authors.initials: C
authors.lastname: Grunau
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002452:Cell Count; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008836:Micrococcal Nuclease / Q000378:metabolism
chemical_list: D000906:Antibodies; D002843:Chromatin; D006657:Histones; D004247:DNA; D008836:Micrococcal Nuclease