title: Acetazolamide inhibits aquaporin-1 expression and colon cancer xenograft tumor growth.
abstract: OBJECTIVE
  To study the effects of water channel protein inhibitor acetazolamide on xenograft tumor growth of colon cancer in nude mice.
  
  
  METHODS
  Setting up human colon cancer model in nude mice, mice were randomly divided into two groups as experimental group and control group. Acetazolamide was given at a volume of 0.1mL per mice (40mg/kg/d, ig) in experimental group, while the same volume of sterile saline was given in control group (ig). After 21 days, protein and m-RNA levels of AQP-1 in tumor tissues from two groups were detected respectively by Western blot and RT-PCR to evaluate the treatment effects. AQP-1, VEGF and CD34 expression was detected by immunohistochemistry, simultaneously.
  
  
  RESULTS
  Acetazolamide (40mg/kg/d, ig) significantly inhibited the xenograft tumor growth of colon cancer in nude mice. The inhibition rate was 88.28%. In comparison with the control group, AQP-1 protein and mRNA level were significantly reduced in the experimental group (p<0.01). AQP-1, VEGF and CD34 expression in experimental group were positively correlated between each other (p<0.01).
  
  
  CONCLUSIONS
  Acetazolamide can suppress the xenograft tumor growth by inhibiting the expression of AQP-1.
authors.affiliation: Department of Coloproctology, Hebei Medical University, Shijiazhuang, China. lzheng77@sina.com
authors.forename: Kong
authors.initials: K
authors.lastname: Bin
authors.forename: Zhao
authors.initials: Z
authors.lastname: Shi-Peng
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology*; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D051398:Aquaporin 1 / Q000378:metabolism*; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000188:drug therapy*; D005260:Female; D007124:Immunoenzyme Techniques; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D060888:Real-Time Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A; D051398:Aquaporin 1; D000086:Acetazolamide