title: [Effect of penetrative needling of scalp-acupoints on cerebral N-acetyl-aspartate and choline levels in intracerebral hemorrhage rabbits].
abstract: OBJECTIVE
  To observe the effect of scalp-acupuncture on N-acetyl-aspartate (NAA) and choline (Cho) leveIs in the brain tissue in rabbits with intracerebral hemorrhage (ICH) so as to explore its underlying mechanisms in resisting neural lesion.
  
  
  METHODS
  A total of 30 Newzealand rabbits were randomly divided into normal, model and acupuncture groups (n=10). ICH model was duplicated by injection of the rabbit-own blood (200 microL) into the bilateral Basal Nuclei (A: 2 mm; R, L; 5 mm; H: 10 mm). A filiform needle was inserted into "Baihui" (GV 20) to "Taiyang" (EX-HN 5) on the hemorrhage side, manipulated for 1 min, and then retained for 30 min (manipulated once again every 10 min). The treatment was conducted once daily for 7 days. Contents of NAA, Cho and creatine (Cr) in the intracerebral peri-hematoma tissues were detected by using 1H-Magnetic Resonance Spectroscopy (MRS).
  
  
  RESULTS
  Compared with the normal group, NAA/Cr ratios at the time points of 2 h, 3 d and 7 d after ICH in the model group were decreased markedly (P < 0.05), while Cho/Cr ratios at the same 3 time-points after ICH in the model group were increased markedly (P < 0.05). In comparison with the model group, NAA/Cr ratio on day 3 after ICH in the acupuncture group was obviously higher (P < 0.05), while Cho/Cr ratios on day 3 and 7 were significantly down-regulated in the acupuncture group (P < 0.05). No significant changes were found in NAA/Cr ratios at 2 h and on day 7 and in Cho/Cr ratio at 2 h after ICH between the acupuncture group and the model group.
  
  
  CONCLUSIONS
  Penetrative acupuncture of GV 20-EX-HN 5 is effective in suppressing ICH-induced increase of Cho/Cr ratio in ICH rabbits, which may contribute to its function in accelerating neural repair process.
authors.affiliation: Yueyang Hospital of Shanghai University of Chinese Medicine, Shanghai 200437, China. haha84122@163.com
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Hui-shu
authors.initials: HS
authors.lastname: Lei
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.forename: Huan-huan
authors.initials: HH
authors.lastname: Li
authors.forename: Gui-rong
authors.initials: GR
authors.lastname: Dong
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000628:therapy*; D002794:Choline / Q000032:analysis*; D003401:Creatine / Q000032:analysis; D008297:Male; D011817:Rabbits; D012535:Scalp
chemical_list: D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline