title: Nanodiscs versus macrodiscs for NMR of membrane proteins.
abstract: It is challenging to find membrane mimics that stabilize the native structures, dynamics, and functions of membrane proteins. In a recent advance, nanodiscs have been shown to provide a bilayer environment compatible with solution NMR. We show that increasing the lipid to "belt" peptide ratio expands their diameter, slows their reorientation rate, and allows the protein-containing discs to be aligned in a magnetic field for oriented sample solid-state NMR. The spectroscopic properties of membrane proteins with one to seven transmembrane helices in q = 0.1 isotropic bicelles, ~10 nm diameter isotropic nanodiscs, ~30 nm diameter magnetically aligned macrodiscs, and q = 5 magnetically aligned bicelles are compared.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0307, United States.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Park
authors.forename: Sabrina
authors.initials: S
authors.lastname: Berkamp
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Cook
authors.forename: Michelle K
authors.initials: MK
authors.lastname: Chan
authors.forename: Hector
authors.initials: H
authors.lastname: Viadiu
authors.forename: Stanley J
authors.initials: SJ
authors.lastname: Opella
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001436:Bacteriorhodopsins / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D008567:Membranes, Artificial; D018716:Molecular Mimicry
chemical_list: D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D001436:Bacteriorhodopsins