title: miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2.
abstract: Induced pluripotent stem cells (iPSCs) can be generated by overexpression of Oct4, Sox2 and Klf4 in murine fibroblasts. By conducting a microRNA (miRNA) library screen, we identified a set of miRNAs critically regulating iPSC formation. We revealed a new miRNA family (miR-130/301/721) as an important regulator of iPSC induction by targeting the homeobox transcription factor Meox2 (also known as Gax). Meox2-specific silencing mimicked the effects of this miRNA family on reprogramming. Mechanistically, miRNA-resistant Meox2 overexpression abrogated effects of miR-130/301/721 on reprogramming. In conclusion, the miRNA family miR-130/301/721 enhances iPSC generation via repression of Meox2.
authors.affiliation: REBIRTH-Group Reprogramming, Hannover Medical School, 30625 Hannover, Germany.
authors.forename: Nils
authors.initials: N
authors.lastname: Pfaff
authors.forename: Jan
authors.initials: J
authors.lastname: Fiedler
authors.forename: Angelika
authors.initials: A
authors.lastname: Holzmann
authors.forename: Axel
authors.initials: A
authors.lastname: Schambach
authors.forename: Thomas
authors.initials: T
authors.lastname: Moritz
authors.forename: Tobias
authors.initials: T
authors.lastname: Cantz
authors.forename: Thomas
authors.initials: T
authors.lastname: Thum
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000235:genetics; D004622:Embryo, Mammalian / Q000166:cytology; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D005820:Genetic Testing; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results
chemical_list: D018398:Homeodomain Proteins; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; C484194:Meox2 protein, mouse; D035683:MicroRNAs