title: 1H, 13C and 15N resonance assignments of the GTPase-activating (GAP) and Ral binding domains (GBD) of RLIP76 (RalBP1).
abstract: RLIP76 (also known as RalBP1) is an effector for Ral small G proteins. RLIP76 is a multifunctional, multi-domain protein that includes a GTPase activating domain for the Rho family (RhoGAP domain) and a GTPase binding domain (GBD) for the Ral small G proteins. The juxtaposition of these two domains (GAP and GBD) may be a strategy employed to co-ordinate regulation of Rho family and Ral-controlled signalling pathways at a crossover node. Here we present the (1)H, (15)N and (13)C NMR backbone and sidechain resonance assignments of the GAP and GBD di-domain (31 kDa).
authors.affiliation: Department of Biochemistry, University of Cambridge, 80, Tennis Court Road, Cambridge CB2 1GA, UK.
authors.forename: Karthik V
authors.initials: KV
authors.lastname: Rajasekar
authors.forename: Louise J
authors.initials: LJ
authors.lastname: Campbell
authors.forename: Daniel
authors.initials: D
authors.lastname: Nietlispach
authors.forename: Darerca
authors.initials: D
authors.lastname: Owen
authors.forename: Helen R
authors.initials: HR
authors.lastname: Mott
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D020690:GTPase-Activating Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011522:Protons
chemical_list: D018528:ATP-Binding Cassette Transporters; D002247:Carbon Isotopes; D020690:GTPase-Activating Proteins; D009587:Nitrogen Isotopes; D011522:Protons; C095637:RALBP1 protein, human