title: [Value of protein array in the diagnosis of Helicobactor pylori infection in children].
abstract: OBJECTIVE
  To study the value of multiple Helicobacter pylori (H.pylori) antibody detection by protein array in the diagnosis of H.pylori infection in children.
  
  
  METHODS
  Biopsy specimens obtained by gastroscopy from 120 children with digestive system symptoms were detected by rapid urease test (RUT) and modified Giemsa staining. Positivity in both RUT and Giemsa staining was the "gold criterion" of H.pylori infection. Serum samples of these patients were obtained and the antibodies against cytotoxin associated gene A protein (CagA), vacuolating toxin A (VacA), urease, heat shock protein 60 (Hsp60) and RdxA (nitroreductase) were detected by protein array technique.
  
  
  RESULTS
  H.pylori infection was identified according to the "gold criterion" in 60 children. Compared with the "gold criterion", the goodness of fit and the coefficient of contingency in the diagnosis of H.pylori infection of the following four groups antibody detection were all statistically significant (P<0.001): anti-Ure antibody alone, anti-Ure antibody combined with anti-CagA antibody, anti-Ure antibody combined with anti-VacA antibody and anti-Ure antibody combined with anti-CagA and anti-VacA antibody. The sensitivity, specificity and accuracy of the detection of anti-Ure antibody combined with anti-CagA antibody for the diagnosis of H.pylori infection were 81.7%, 91.7% and 86.7%, respectively. The antibody detection showed a high positive predictive value (90.7%) and a high negative predictive value (83.3%).
  
  
  CONCLUSIONS
  The antibody detection by protein array, especially the detection of anti-Ure antibody combined with anti-CagA antibody, is valuable in the diagnosis of H.pylori infection.
authors.affiliation: Department of Gastrointestinal Laboratory, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Shu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Cheng
authors.forename: Ling-Rong
authors.initials: LR
authors.lastname: Yang
authors.forename: Xu-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Yan-Yi
authors.initials: YY
authors.lastname: Zhang
authors.forename: Mi-Zu
authors.initials: MZ
authors.lastname: Jiang
mesh_terms: D000293:Adolescent; D000907:Antibodies, Bacterial / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D040081:Protein Array Analysis / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial