title: Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato.
abstract: A resveratrol synthase gene was cloned from the peanut plant (Arachis hypogaea) by RT-PCR and was transformed into purple sweet potato (Ipomoea batatas) by Agrobacterium-mediated transformation. Stem sections were infected with bacterial solution of OD(600) = 0.4 for 20 min and then cocultured for 2 days. Infected explants were cultured on MS media containing 50 mg/l kanamycin, 0.02 mg/l NAA and 1 mg/l 6-BA for bud induction or containing 75 mg/l kanamycin, 1.0 mg/l NAA and 0.1 mg/l 6-BA for root formation. The bud and root induction rates were 37.5 and 25.0%, respectively. 105 regenerated plants were obtained, with 11 positive plants by PCR and Southern blotting analyses. A high level of resveratrol glucoside (340 μg/g dry weight), but no resveratrol, was detected in the transformed plants by HPLC. This study also provides a stable genetic transformation and plant regeneration method for metabolic modification of purple sweet potato.
authors.affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou, China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Pan
authors.forename: Si-Li
authors.initials: SL
authors.lastname: Yu
authors.forename: Chang-Jian
authors.initials: CJ
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Yi-Liang
authors.initials: YL
authors.lastname: Wu
authors.forename: Hai-Hang
authors.initials: HH
authors.lastname: Li
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D060054:Agrobacterium / Q000235:genetics; D010367:Arachis / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D027723:Ipomoea batatas / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics; D000077185:Resveratrol; D017421:Sequence Analysis; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D013267:Stilbenes; D007612:Kanamycin; D000217:Acyltransferases; C055200:resveratrol synthase; D000077185:Resveratrol