title: Prolonged zymosan-induced inflammatory pain hypersensitivity in mice lacking glycine receptor alpha2.
abstract: Glycinergic synapses play a major role in shaping the activity of spinal cord neurons under normal conditions and during persistent pain. However, the role of different glycine receptor (GlyR) subtypes in pain processing has only begun to be unraveled. Here, we analysed whether the GlyR alpha2 subunit might be involved in the processing of acute or persistent pain. Real-time RT-PCR and in situ hybridization analyses revealed that GlyR alpha2 mRNA is enriched in the dorsal horn of the mouse spinal cord. Mice lacking GlyR alpha2 (Glra2(-/-) mice) demonstrated a normal nociceptive behavior in models of acute pain and after peripheral nerve injury. However, mechanical hyperalgesia induced by peripheral injection of zymosan was significantly prolonged in Glra2(-/-) mice as compared to wild-type littermates. We conclude that spinal GlyRs containing the alpha2 subunit exert a previously unrecognized role in the resolution of inflammatory pain.
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Goethe-Universität, 60590 Frankfurt am Main, Germany.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Kallenborn-Gerhardt
authors.forename: Ruirui
authors.initials: R
authors.lastname: Lu
authors.forename: Jana
authors.initials: J
authors.lastname: Lorenz
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Jessica
authors.initials: J
authors.lastname: Weiland
authors.forename: Domenico
authors.initials: D
authors.lastname: Del Turco
authors.forename: Thomas
authors.initials: T
authors.lastname: Deller
authors.forename: Bodo
authors.initials: B
authors.lastname: Laube
authors.forename: Heinrich
authors.initials: H
authors.lastname: Betz
authors.forename: Gerd
authors.initials: G
authors.lastname: Geisslinger
authors.forename: Achim
authors.initials: A
authors.lastname: Schmidtko
mesh_terms: D000818:Animals; D006930:Hyperalgesia / Q000139:chemically induced / Q000235:genetics* / Q000378:metabolism; D007249:Inflammation / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism; D018009:Receptors, Glycine / Q000235:genetics* / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism; D015054:Zymosan / Q000494:pharmacology*
chemical_list: C501173:Glra2 protein, mouse; D018009:Receptors, Glycine; D015054:Zymosan