title: HepG2 cells expressing microRNA miR-122 support the entire hepatitis C virus life cycle.
abstract: The liver-specific microRNA miR-122 is required for efficient hepatitis C virus (HCV) RNA replication both in cell culture and in vivo. In addition, nonhepatic cells have been rendered more efficient at supporting this stage of the HCV life cycle by miR-122 expression. This study investigated how miR-122 influences HCV replication in the miR-122-deficient HepG2 cell line. Expression of this microRNA in HepG2 cells permitted efficient HCV RNA replication and infectious virion production. When a missing HCV receptor is also expressed, these cells efficiently support viral entry and thus the entire HCV life cycle.
authors.affiliation: Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Narbus
authors.forename: Benjamin
authors.initials: B
authors.lastname: Israelow
authors.forename: Marion
authors.initials: M
authors.lastname: Sourisseau
authors.forename: Maria L
authors.initials: ML
authors.lastname: Michta
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Hopcraft
authors.forename: Gusti M
authors.initials: GM
authors.lastname: Zeiner
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Evans
mesh_terms: D015870:Gene Expression; D056945:Hep G2 Cells; D016174:Hepacivirus / Q000254:growth & development / Q000502:physiology*; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D053586:Virus Internalization; D057074:Virus Release; D014779:Virus Replication
chemical_list: C531907:MIRN122 microRNA, human; D035683:MicroRNAs