title: The correlation between resting serum leptin and serum angiogenic indices at rest and after submaximal exercise.
abstract: BACKGROUND
  The effect of leptin as stimulant angiogenic factor has been studied. But the association of leptin levels and exercise-induced angiogenesis has not been studied. Accordingly, the researchers investigated whether there were any differences in circulating serum VEGF, MMP-2 and MMP-9 among high and low resting leptin individuals at rest or in response to submaximal exercise.
  
  
  METHODS
  For this purpose the researchers defined two groups with high and low resting leptin levels. Fifteen subjects with high resting leptin (23.57±9.14ng/ml and Vo(2) max=29.46±3.62ml/kg.min) and fifteen subjects with low resting leptin level (1.04±0.49ng/ml and Vo(2) max=37.99±4.63ml/kgmin) exercised for 1h (1h) at 70% of Vo(2) max. Antecubital vein blood was collected at rest, immediately and 2h post exercise. Serum VEGF, MMP-2 and MMP-9 was measured by ELISA method.
  
  
  RESULTS
  Results of the study showed that the resting serum levels of VEGF, MMP-2 and MMP-9 didn't have any correlation with basic levels of leptin. In low leptin group the levels of VEGF and MMP-2 in immediately post exercise decreased significantly, but in high leptin group, only VEGF decreased significantly. 2h post exercise; the VEGF level in the low resting leptin group was significantly lower than that of its basal level. Beside, MMP-2 in the high and low basic levels of leptin groups were significantly increased compared to that of immediately post exercise. But the amount of MMP-9 did not change significantly in response to exercise in two groups. There were not any differences in the changes of VEGF, MMP-2 and MMP-9 in response to exercise between two groups. Furthermore, resting leptin had a significant correlation with V0(2) max.
  
  
  CONCLUSIONS
  The obtained results showed that the serum VEGF, MMP-2 and MMP-9 did not have any correlation with basic levels of leptin. In addition, it was concluded that levels of different resting leptin is ineffective on serum levels of VEGF, MMP-2 and MMP-9 at rest and in response to exercise in normal healthy subjects.
authors.affiliation: Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Shahid Beheshti University, G.C., Evin 198396113, Tehran, Iran. m-nourshahi@sbu.ac.ir
authors.forename: Maryam
authors.initials: M
authors.lastname: Nourshahi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hedayati
authors.forename: Kamal
authors.initials: K
authors.lastname: Ranjbar
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood*; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D018919:Neovascularization, Physiologic; D005082:Physical Exertion; D012146:Rest; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*; D055815:Young Adult
chemical_list: D020738:Leptin; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9