title: Effects of hemodilution after traumatic brain injury in juvenile rats.
abstract: BACKGROUND
  Normovolemic hemodilution (HD) in adult animal studies has shown exacerbation of traumatic brain injury (TBI) lesion volumes. Similar studies in juvenile rats have not been reported and outcomes are likely to be different. This study investigated the effects of normovolemic hemodilution (21% hematocrit) in a juvenile TBI (jTBI) model.
  
  
  METHODS
  Twenty 17-day-old rats underwent moderate cortical contusion impact injury (CCI) and were divided into four groups: CCI/hemodilution (HD) (group HD), CCI/no HD (group C), Sham/HD (group SHD), and Sham/no HD (group S). Regional laser Doppler flowmetry (LDF), edema formation (MRI-T2WI), water mobility assessed using diffusion weighted imaging (MRI-DWI), open field activity tests, and histological analyses were evaluated for lesion characteristics.
  
  
  RESULTS
  Hemodilution significantly increased blood flow in the HD compared to the C group after TBI. T2WI revealed a significantly increased extravascular blood volume in HD at 1, 7, and 14 days post-CCI. Edematous tissue and total contusional lesion volume were higher in HD-treated animals at 1 and 14 days. DWI revealed that HD, SHD, and C groups had elevated water mobility compared to S groups in the ipsilateral cortex and striatum. Histology showed a larger cortical lesion in the C than HD group. Open field activity was increased in HD, C, and SHD groups compared to the S group.
  
  
  CONCLUSIONS
  Hemodilution results in significant brain hyperemia with increased edema formation, extravascular blood volume, and water mobility after jTBI. Hemodilution results in less cortical damage but did not alter behavior. Hemodilution is likely not to be clinically beneficial following jTBI.
authors.affiliation: Department of Anesthesiology, Loma Linda University, Loma Linda, CA 92354, USA.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Bertolizio
authors.forename: Bruno
authors.initials: B
authors.lastname: Bissonnette
authors.forename: Linda
authors.initials: L
authors.lastname: Mason
authors.forename: Stephen
authors.initials: S
authors.lastname: Ashwal
authors.forename: Richard
authors.initials: R
authors.lastname: Hartman
authors.forename: Suzzanne
authors.initials: S
authors.lastname: Marcantonio
authors.forename: Andre
authors.initials: A
authors.lastname: Obenaus
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology / Q000503:physiopathology; D001930:Brain Injuries / Q000473:pathology* / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods; D004195:Disease Models, Animal; D004487:Edema / Q000503:physiopathology; D006400:Hematocrit; D006438:Hemodilution / Q000379:methods*; D017078:Laser-Doppler Flowmetry; D008297:Male; D009043:Motor Activity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000008:administration & dosage
chemical_list: D012965:Sodium Chloride