title: Evaluation of protective efficacy of respiratory syncytial virus vaccine against A and B subgroup human isolates in Korea.
abstract: Human respiratory syncytial virus (HRSV) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. HRSV is divided into two subgroups, HRSV-A and HRSV-B, based on sequence variation within the G gene. Despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for HRSV. In this study, we have detected and identified the HRSV by RT-PCR from nasopharyngeal aspirates of Korean pediatric patients. Interestingly, all HRSV-B isolates exhibited unique deletion of 6 nucleotides and duplication of 60 nucleotides in the G gene. We successfully amplified two isolates ('KR/A/09-8' belonging to HRSV-A and 'KR/B/10-12' to HRSV-B) on large-scale, and evaluated the cross-protective efficacy of our recombinant adenovirus-based HRSV vaccine candidate, rAd/3xG, by challenging the immunized mice with these isolates. The single intranasal immunization with rAd/3xG protected the mice completely from KR/A/09-8 infection and partially from KR/B/10-12 infection. Our study contributes to the understanding of the genetic characteristics and distribution of subgroups in the seasonal HRSV epidemics in Korea and, for the first time, to the evaluation of the cross-protective efficacy of RSV vaccine against HRSV-A and -B field-isolates.
authors.affiliation: Division of Life and Pharmaceutical Sciences, Center for Cell Signaling and Drug Discovery Research, Ewha Womans University, Seoul, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Jang
authors.forename: Jee-Boong
authors.initials: JB
authors.lastname: Lee
authors.forename: Kyung-Hyo
authors.initials: KH
authors.lastname: Kim
authors.forename: Sung Moo
authors.initials: SM
authors.lastname: Park
authors.forename: Byoung-Shik
authors.initials: BS
authors.lastname: Shim
authors.forename: In Soo
authors.initials: IS
authors.lastname: Cheon
authors.forename: Man Ki
authors.initials: MK
authors.lastname: Song
authors.forename: Jun
authors.initials: J
authors.lastname: Chang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008168:Lung / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D056910:Republic of Korea; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*; D012136:Respiratory Syncytial Viruses / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D022261:Respiratory Syncytial Virus Vaccines