title: [Studies on the oxidation of tyrosine induced by hydroxyl radical with fluorescence spectroscopic method].
abstract: Dityrosine is a marker of tyrosine oxidation. To study effecting factors of hydroxyl radical on tyrosine oxidation, synchronous fluorescence spectra with two dimensional correlation was used. The results showed that the peak position and intensity of dityrosine changed while pH value varied. In the system of tyrosine oxidation, with the increment of tyrosine concentration, the concentration of dityrosine decreased. With the increment of hydrogen peroxide concentration, the concentration of dityrosine increased. The oxidation reaction was prone to taking place in acid conditions while difficult to develop in basic conditions. With the development of oxidation reaction, the fluorescence intensity of dityrosine increased and then decreased. Two dimentional correlation synchronous fluorescence spectra showed that the variation in the intensity at 292 nm preceded that of 281, 300 and 374 nm. Thus, fluorescence spectroscopy was simple and easy for studying tyrosine oxidation induced by hydroxyl radical.
authors.affiliation: Department of Chemistry and Life Sciences, Chuzhou University, Chuzhou. syh2004@126.com
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Sun
authors.forename: Wei-long
authors.initials: WL
authors.lastname: Wang
authors.forename: Lin-sheng
authors.initials: LS
authors.lastname: Wu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Jia
mesh_terms: D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical / Q000737:chemistry*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D017665:Hydroxyl Radical; D014443:Tyrosine; D006861:Hydrogen Peroxide; C007543:dityrosine