title: Molecular characterization of porcine MMP19 and MMP23B genes and its association with immune traits.
abstract: MMP19 and MMP23B belong to the Matrix metalloproteases (MMPs) family, which are zinc-binding endopeptidases that are capable of degrading various components of the extracellular matrix. They are thought to play important roles in embryonic development, reproduction and tissue remodeling, as well as in cell proliferation, differentiation, migration, angiogenesis, apoptosis and host defense. However, they are poorly understood in pigs. Here, we obtained the full length coding region sequence and genomic sequence of the porcine MMP19 and MMP23B genes and analyzed their genomic structures. The deduced amino acid sequence shares similar precursor protein domains with human and mouse MMP19 and MMP23B protein, respectively. Using IMpRH panel, MMP19 was mapped to SSC5p12-q11 (closely linked to microsatellite DK) and MMP23B was mapped to SSC8q11-q12 (linked to microsatellite Sw2521). Quantitative real-time PCR showed that MMP19 was abundantly expressed in the liver, while MMP23B was strongly expressed in the ovarian and heart. Furthermore, both genes were all expressed increasingly in prenatal skeletal muscle during development. Three SNPs were detected by sequencing and PCR-RFLP methods, and association analysis indicated that C203T at exon 5 of MMP19 has a significant association with the blood parameters WBC (G/L) and IgG2 (mg/mL) (P<0.05), SNP C131T at exon 3 of MMP23B is significantly associated with the blood parameters HGB (g/L) and MCH (P<0.05), and A150G in exon 4 has no significant association with the economic traits in pigs.
authors.affiliation: College of Animal Science and Technology, Northwest A & F University, No. 22 Xinong Road, 712100 Yangling, Shaanxi, China.
authors.forename: Shuanping
authors.initials: S
authors.lastname: Zhao
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Zhao
authors.forename: Pengxia
authors.initials: P
authors.lastname: Niu
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Tang
authors.forename: Linsen
authors.initials: L
authors.lastname: Zan
authors.forename: Kui
authors.initials: K
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D003001:Cloning, Molecular; D005787:Gene Frequency; D056726:Genetic Association Studies; D020782:Matrix Metalloproteinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D053505:Matrix Metalloproteinases, Secreted / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology
chemical_list: D020782:Matrix Metalloproteinases; D053505:Matrix Metalloproteinases, Secreted; C108362:matrix metalloproteinase 19