title: GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity.
abstract: A spontaneous non-pathogenic variant (Cong:1-2) derived from Fusarium oxysporum f. sp. conglutinans (Cong: 1-1), a causal agent of cabbage yellows, carries biocontrol activity for cabbage yellows. We found a GMC oxidoreductase (ODX1) among the proteins expressed much more in Cong:1-2 than Cong:1-1 by 2D-DIGE comparison. GMC oxidoreductases have been reported to be involved in biocontrol activity of several plant pathogenic fungi. The gene encoding ODX1 in Cong:1-2 was cloned, and targeted disruption of the gene in Cong:1-2 did not affect its biocontrol activity, suggesting that GMC oxidoreductase is dispensable for biocontrol activity in the fungal biocontrol agent.
authors.affiliation: Research Team for Detection of Plant Pathogens and Nematodes, National Agriculture Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Kawabe
authors.forename: Akiko
authors.initials: A
authors.lastname: Okabe Onokubo
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Arimoto
authors.forename: Takanobu
authors.initials: T
authors.lastname: Yoshida
authors.forename: Koji
authors.initials: K
authors.lastname: Azegami
authors.forename: Tohru
authors.initials: T
authors.lastname: Teraoka
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Arie
mesh_terms: D001937:Brassica / Q000382:microbiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010572:Pest Control, Biological; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D036226:Seedlings / Q000382:microbiology; D057825:Two-Dimensional Difference Gel Electrophoresis; D014774:Virulence
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D010088:Oxidoreductases