title: [Phosphorylation of histone H2AX in human lymphocytes as a possible marker of effective cellular response to ionizing radiation].
abstract: DNA double-strand breaks (DSBs) which occurs in cells after ionizing radiation (IR) or chemical agents are the most dangerous lesions in eukariotic cells, which leads to cell death or chromosome abberations and cancer. One of the earliest response of cells to DSBs formation is phosphorylation by 139 serine of core variant histone H2AX in megabase chromatin domains around DSB (gamma-H2AX), which amplify signal and makes it possible to identify even one DSB in genome. Effective formation of gamma-H2AX is very important for maintenance of genome stability. Here, using immunofluorescent and Western blotting techniques, we studied dynamics of gamma-H2AX formation in human lymphocytes of various individuals irradiated ex vivo. We have found that dynamics of gamma-H2AX formation in lymphocytes differ between individuals but have similar kinetics and statistically is independent on people age.
authors.forename: D V
authors.initials: DV
authors.lastname: Firsanov
authors.forename: A V
authors.initials: AV
authors.lastname: Kropotov
authors.forename: N V
authors.initials: NV
authors.lastname: Tomilin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002877:Chromosomes, Human / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects*; D005260:Female; D042822:Genomic Instability / Q000528:radiation effects*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010766:Phosphorylation / Q000528:radiation effects; D014965:X-Rays / Q000009:adverse effects*
chemical_list: D015415:Biomarkers; C468783:H2AX protein, human; D006657:Histones