title: Paraquat administration in Drosophila for use in metabolic studies of oxidative stress.
abstract: Paraquat (PQ) is widely used in the laboratory to induce in vivo oxidative stress, particularly in the fruit fly, Drosophila melanogaster. PQ administration to the fly traditionally involves feeding in a 1% sucrose solution; however, a diet high in sucrose can itself be stressful. We examined a novel method of PQ administration: incorporation into the fly's standard cornmeal-sucrose-yeast diet. This method successfully delivers PQ to the fly at concentrations similar to those of the traditional method but with fewer possibly confounding complications.
authors.affiliation: Department of Chemistry and Biochemistry, Laurentian University, Sudbury, Ontario, Canada P3E 2C6.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Rzezniczak
authors.forename: L A
authors.initials: LA
authors.lastname: Douglas
authors.forename: J H
authors.initials: JH
authors.lastname: Watterson
authors.forename: T J S
authors.initials: TJ
authors.lastname: Merritt
mesh_terms: D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D010269:Paraquat