title: Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells.
abstract: We have previously reported that the dynein light chains km23-1 and km23-2 are required for TGFβ signaling in mammalian cells. Here we describe another member of the km23/DYNLRB/LC7/robl family of dynein light chains in zebrafish, termed zkm23, which is also involved in TGFβ signaling. zkm23 was rapidly phosphorylated after TGFβ stimulation. TGFβ RII kinase activity was absolutely required for zkm23 phosphorylation, whereas a constitutively active TGFβ RI did not induce phosphorylation. Further, TGFβ stimulated a rapid recruitment of the zkm23 dynein light chain to the dynein intermediate chain of the dynein complex, and the TGFβ RII kinase was required for this interaction. Finally, blockade of zkm23 using morpholino oligos resulted in an inhibition of TGFβ-mediated transcriptional responses. Thus, our results demonstrate for the first time that the dynein light chain zkm23 is required for TGFβ signaling in cultured zebrafish ovarian follicle cells.
authors.affiliation: Department of Biochemistry and Molecular Biology, Penn State Hershey, College of Medicine, Hershey, PA 17033, USA.
authors.forename: Qunyan
authors.initials: Q
authors.lastname: Jin
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gao
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Mulder
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004398:Dyneins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D040461:Gene Components; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation; D061251:Primary Cell Culture; D011485:Protein Binding; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins; C570382:dynlrb1 protein, zebrafish; D049410:Luciferases, Renilla; D004398:Dyneins