title: Immortalized chick embryo cell line adapted to serum-free growth conditions and capable of replicating human and reassortant H5N1 influenza strains for vaccine production.
abstract: The current process of influenza vaccine production can take 6-9 months and is dependent on the availability of embryonated eggs. Additionally, this process selects for receptor-binding variants with reduced antigenicity and requires significant downstream production for purification. We have established an immortalized chick embryo cell line, termed PBS-12SF, which is adapted to growth in serum free conditions, and is capable of replicating human and reassortant H5N1 influenza strains to high titers. In many cases, PBS-12SF cells produced higher growth titers of influenza virus than those of primary chick embryo kidney (CEK) cells, Madin-Darby Canine Kidney (MDCK) cells and African green monkey kidney cells (Vero). Additionally, in PBS-12SF cell cultures, influenza virus is released into the culture fluid without need for exogenous proteases, which can simplify downstream processing for vaccine production.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA. coussens@msu.edu
authors.forename: Paul M
authors.initials: PM
authors.lastname: Coussens
authors.forename: Kristen A
authors.initials: KA
authors.lastname: Smith
authors.forename: Patty S D
authors.initials: PS
authors.lastname: Weber
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Colvin
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002642:Chick Embryo; D016895:Culture Media, Serum-Free; D053124:Influenza A Virus, H5N1 Subtype / Q000254:growth & development*; D007252:Influenza Vaccines / Q000276:immunology* / Q000302:isolation & purification*; D019562:Viral Load; D014776:Virus Cultivation / Q000379:methods
chemical_list: D016895:Culture Media, Serum-Free; D007252:Influenza Vaccines