title: Protein expression regulation under oxidative stress.
abstract: Oxidative stress is known to affect both translation and protein turnover, but very few large scale studies describe protein expression under stress. We measure protein concentrations in Saccharomyces cerevisiae over the course of 2 h in response to a mild oxidative stress induced by diamide, providing detailed time-resolved information for 815 proteins, with additional data for another ~1,100 proteins. For the majority of proteins, we discover major differences between the global transcript and protein response. Although mRNA levels often return to baseline 1 h after treatment, protein concentrations continue to change. Integrating our data with features of translation and protein degradation, we are able to predict expression patterns for 41% of the proteins in the core data set. Predictive features include, among others, targeting by RNA-binding proteins (Lhp1 and Khd1), RNA secondary structures, RNA half-life, and translation efficiency under unperturbed conditions and in response to oxidative reagents, but not chaperone binding. We are able to both describe general dynamics of protein concentration changes and suggest possible regulatory mechanisms for individual proteins.
authors.affiliation: Center for Genomics and Systems Biology, New York University, New York, New York 10003, USA. cvogel@nyu.edu
authors.forename: Christine
authors.initials: C
authors.lastname: Vogel
authors.forename: Gustavo Monteiro
authors.initials: GM
authors.lastname: Silva
authors.forename: Edward M
authors.initials: EM
authors.lastname: Marcotte
mesh_terms: D015966:Gene Expression Regulation, Fungal; D006207:Half-Life; D018384:Oxidative Stress; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis; D055550:Protein Stability; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000235:genetics
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D010544:Peroxidases; C496253:Tsa1 protein, S cerevisiae