title: Rapid detection of Tembusu virus by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP).
abstract: A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of Tembusu virus (TMUV) infection. The reaction was performed in one step in a single tube at 64°C for 45 min, with SYBR Green I dye added prior to amplification. The detection limit of the RT-LAMP assay was approximately 10 copies/μl, and no cross-reaction with other avian viruses was observed. The assay was evaluated further for the diagnosis of TMUV in field samples and compared with conventional RT-PCR, demonstrating that results of the RT-LAMP assay corresponded to those of conventional RT-PCR. In conclusion, RT-LAMP with SYBR Green I dye was shown to be a sensitive, simple assay for the rapid diagnosis of TMUV infection in ducks.
authors.affiliation: Zoology Institute, Agricultural University of Shan Dong province, Shan Dong province, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Diao
authors.forename: C
authors.initials: C
authors.lastname: Yu
authors.forename: X
authors.initials: X
authors.lastname: Gao
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004372:Ducks; D005416:Flavivirus / Q000302:isolation & purification*; D018177:Flavivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000821:virology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity