title: Ethylene-responsive element-binding factor 5, ERF5, is involved in chitin-induced innate immunity response.
abstract: Our recent work demonstrated that chitin treatment modulated the expression of 118 transcription factor (TF) genes in Arabidopsis. To investigate the potential roles of these TF in chitin signaling and plant defense, we initiated an interaction study among these TF proteins, as well as two chitin-activated mitogen-activated protein kinases (MPK3 and MPK6), using a yeast two-hybrid system. This study revealed interactions among the following proteins: three ethylene-responsive element-binding factors (ERF), five WRKY transcription factors, one scarecrow-like (SCL), and the two MPK, in addition to many other interactions, reflecting a complex TF interaction network. Most of these interactions were subsequently validated by other methods, such as pull-down and in planta bimolecular fluorescence complementation assays. The key node ERF5 was shown to interact with multiple proteins in the network, such as ERF6, ERF8, and SCL13, as well as MPK3 and MPK6. Interestingly, ERF5 appeared to negatively regulate chitin signaling and plant defense against the fungal pathogen Alternaria brassicicola and positively regulate salicylic acid signaling and plant defense against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Therefore, ERF5 may play an important role in plant innate immunity, likely through coordinating chitin and other defense pathways in plants in response to different pathogens.
authors.affiliation: University of Missouri, Columbia, MO, USA.
authors.forename: Geon Hui
authors.initials: GH
authors.lastname: Son
authors.forename: Jinrong
authors.initials: J
authors.lastname: Wan
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Xuan Canh
authors.initials: XC
authors.lastname: Nguyen
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Chung
authors.forename: Jong Chan
authors.initials: JC
authors.lastname: Hong
authors.forename: Gary
authors.initials: G
authors.lastname: Stacey
mesh_terms: D000528:Alternaria / Q000502:physiology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002686:Chitin / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D054884:Host-Pathogen Interactions; D018546:Hypocotyl / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000502:physiology; D007113:Immunity, Innate; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D054883:Oxylipins / Q000494:pharmacology; D010766:Phosphorylation; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000502:physiology; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping; D044224:Pseudomonas syringae / Q000502:physiology; D020156:Salicylic Acid / Q000494:pharmacology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000276:immunology / Q000502:physiology; D015398:Signal Transduction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C582788:AT5G47230 protein, Arabidopsis; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D014157:Transcription Factors; D002686:Chitin; C011006:jasmonic acid; C036216:ethylene; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid