title: Role of T and dendritic cells in mouse islet allografts treated with anti-CD45RB monoclonal antibodies.
abstract: Currently lifelong immunosuppression is required for organ transplant recipients. Anti-CD45RB monoclonal antibody (mAb) prolongs graft survival by mechanisms that are not yet clear. Therefore, we investigated the role of T and dendritic cells (DC) in islet allografts treated with anti-CD45RB mAb after transplantation of 200 allogeneic islets (BALB/c mouse) under the kidney capsules of diabetic C57BL/6 mice treated with intraperitoneal injections of 100 μg of anti-CD45RB mAb on days 0, 1, 3, 5, and 7. We observed a tilt of the ratios of Th1/Th2 and Tc1/Tc2 to Th2 and Tc2. The numbers of naïve and memory T cells were down-regulated in peripheral blood after transplantation. In addition, the maturation, endocytosis, and interleukin-12 secreted by DC derived from bone marrow cells was suppressed in recipient mice. Therefore, anti-CD45RB mAb alleviated, rejection by suppressive effects on T-lymphocyte subsets and DC.
authors.affiliation: Clinical Medical Research Center, the Second Clinical Medical College (Shenzhen People's Hospital) Ji'nan University, Shenzhen, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Qi
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.forename: H X
authors.initials: HX
authors.lastname: Zhou
authors.forename: S P
authors.initials: SP
authors.lastname: Deng
authors.forename: F R
authors.initials: FR
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology; D005434:Flow Cytometry; D007156:Immunologic Memory; D007515:Islets of Langerhans / Q000276:immunology*; D017493:Leukocyte Common Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013311:Streptozocin; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D013311:Streptozocin; D017493:Leukocyte Common Antigens