title: A hydrogel-mineral composite scaffold for osteochondral interface tissue engineering.
abstract: Osteoarthritis is the leading cause of physical disability among Americans, and tissue engineered cartilage grafts have emerged as a promising treatment option for this debilitating condition. Currently, the formation of a stable interface between the cartilage graft and subchondral bone remains a significant challenge. This study evaluates the potential of a hybrid scaffold of hydroxyapatite (HA) and alginate hydrogel for the regeneration of the osteochondral interface. Specifically, the effects of HA on the response of chondrocytes were determined, focusing on changes in matrix production and mineralization, as well as scaffold mechanical properties over time. Additionally, the optimal chondrocyte population for interface tissue engineering was evaluated. It was observed that the HA phase of the composite scaffold promoted the formation of a proteoglycan- and type II collagen-rich matrix when seeded with deep zone chondrocytes. More importantly, the elevated biosynthesis translated into significant increases in both compressive and shear moduli relative to the mineral-free control. Presence of HA also promoted chondrocyte hypertrophy and type X collagen deposition. These results demonstrate that the hydrogel-calcium phosphate composite supported the formation of a calcified cartilage-like matrix and is a promising scaffold design for osteochondral interface tissue engineering.
authors.affiliation: Biomaterials and Interface Tissue Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA.
authors.forename: Nora T
authors.initials: NT
authors.lastname: Khanarian
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Leo Q
authors.initials: LQ
authors.lastname: Wan
authors.forename: Van C
authors.initials: VC
authors.lastname: Mow
authors.forename: Helen H
authors.initials: HH
authors.lastname: Lu
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D024121:Collagen Type X / Q000096:biosynthesis; D017886:Durapatite / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D010003:Osteoarthritis / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D024121:Collagen Type X; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; D017886:Durapatite