title: Characterization of the expression and activity of carboxylesterases 1 and 2 from the beagle dog, cynomolgus monkey, and human.
abstract: The carboxylesterases (CESs) are a family of serine hydrolases that hydrolyze compounds containing an ester, amide, or thioester. In humans, two dominant forms, CES1 and CES2, are highly expressed in organs of first-pass metabolism and play an important role in xenobiotic metabolism. The current study was conducted to better understand species-related differences in substrate selectivity and tissue expression of these enzymes. To elucidate potential similarities and differences among these enzymes, a series of 4-nitrophenyl esters and a series of gemcitabine prodrugs were evaluated using enzyme kinetics as substrates of expressed and purified CESs from beagle dog, cynomolgus monkey, and human genes. For the substrates examined, human and monkey CES2 more efficiently catalyzed hydrolysis compared with CES1, whereas CES1 was the more efficient enzyme in dog. Quantitative real-time polymerase chain reaction and Western blot analyses indicate that the pattern of CES tissue expression in monkey is similar to that of human, but the CES expression in dog is unique, with no detectable expression of CES in the intestine. Loperamide, a selective human CES2 inhibitor, was also found to be a CES2-selective inhibitor in both dog and monkey. This is the first study to examine substrate specificity among dog, human, and monkey CESs.
authors.affiliation: Department of Drug Disposition, Eli Lilly and Company, Lilly Research Laboratories, Indianapolis, IN 46285, USA.
authors.forename: Eric T
authors.initials: ET
authors.lastname: Williams
authors.forename: James A
authors.initials: JA
authors.lastname: Bacon
authors.forename: David M
authors.initials: DM
authors.lastname: Bender
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Lowinger
authors.forename: Wen-Kai
authors.initials: WK
authors.lastname: Guo
authors.forename: Mariam E
authors.initials: ME
authors.lastname: Ehsani
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Yue-Wei
authors.initials: YW
authors.lastname: Qian
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Ruterbories
authors.forename: Steven A
authors.initials: SA
authors.lastname: Wrighton
authors.forename: Everett J
authors.initials: EJ
authors.lastname: Perkins
mesh_terms: D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D013379:Substrate Specificity
chemical_list: D002265:Carboxylic Ester Hydrolases