title: Cationic polydiacetylene micelles for gene delivery.
abstract: Cationic surfactants easily interact with plasmid DNA to form small lipoplexes. However, their detergent behavior and associated biological toxicity limit their use as gene delivery vectors. We have incorporated a diacetylene motif in the hydrophobic chain of cationic surfactants. By using UV irradiation, the small cationic micelles (9 nm) obtained with diacetylenic detergents were photopolymerized into 40 nm spheres. Electrostatic interactions with plasmid DNA led to the formation of 45 nm lipoplexes at N/P = 5 ratio. In vitro transfection of the pCMV-Luciferase plasmid resulted in gene expression (>10(10) RLU/mg protein) at the same ratio, comparable with the commercially available JetSi-ENDO gene delivery system. This new and versatile class of molecules could lead to a new generation of in vivo gene delivery vectors.
authors.affiliation: Laboratory of Functional Chemo Systems, CAMB, CNRS-UDS UMR 7199, Faculté de Pharmacie, Université de Strasbourg, Illkirch, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Morin
authors.forename: Marc
authors.initials: M
authors.lastname: Nothisen
authors.forename: Alain
authors.initials: A
authors.lastname: Wagner
authors.forename: Jean-Serge
authors.initials: JS
authors.lastname: Remy
mesh_terms: D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008823:Micelles; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D000078789:Polyacetylene Polymer; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D053279:Polyynes / Q000138:chemical synthesis / Q000737:chemistry*; D014162:Transfection
chemical_list: D002412:Cations; D008823:Micelles; D000078789:Polyacetylene Polymer; D011108:Polymers; D053279:Polyynes; C082361:polydiacetylene; D004247:DNA; D008156:Luciferases