title: [Detection and analysis of comE and luxS genes in quorum sensing signal pathway from Streptococcus oralis].
abstract: OBJECTIVE
  To detect and analyze two important genes, comE and luxS, in quorum sensing signal pathway from Streptococcus oralis (S. oralis).
  
  
  METHODS
  The total genomic DNA of S. oralis NH521 (a clinically isolated strain) was firstly extracted. The comE and luxS genes were then amplified by polymerase chain reaction (PCR) and further sequenced. The obtained sequences were compared with related sequences in GenBank.
  
  
  RESULTS
  Target bands of both comE and luxS genes were detected by electrophoresis. The obtained gene sequences were similar to the corresponding sequences from another S. oralis strain (luxS, 95.0%; comE, 99.6%); however, comparing to gene sequences of another species Streptococcus mutans, comE was more divergent (12.7%) than luxS gene (74.1%).
  
  
  CONCLUSIONS
  This study successfully amplified and sequenced comE and luxS genes from S. oralis NH521 strain. The luxS gene accumulated more mutations than comE gene did between two S. oralis strains, but comE gene is more divergent than luxS gene between two Streptococcus species.
authors.affiliation: Dept. of Oral Medicine, School of Dentistry, Nanjing University, Nanjing 210008, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Jiuyu
authors.initials: J
authors.lastname: Ge
mesh_terms: D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases; D016133:Polymerase Chain Reaction; D053038:Quorum Sensing; D015398:Signal Transduction; D013295:Streptococcus mutans; D019533:Streptococcus oralis
chemical_list: D001426:Bacterial Proteins; D013437:Carbon-Sulfur Lyases