title: Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity.
abstract: We conducted cell-based cytotoxicity screening of a 101-membered α-helical peptide library using cell fingerprints (CFPs). The CFP data suggested that there is a relationship between cytotoxicity and peptide characteristics, such as hydrophobicity, charge, and amino acid composition. In spite of the small size of the library used in this study, several peptides demonstrated cell-specific toxicity. The strategy of combining a designed peptide library with CFP thus shows real promise for peptide-based screening with cells.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kusui@center.konan-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Usui
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakiyama
authors.forename: Kin-Ya
authors.initials: KY
authors.lastname: Tomizaki
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Mihara
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D002942:Circular Dichroism; D010455:Peptides / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010455:Peptides