title: Isolation, characterization, and cross-amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex.
abstract: OBJECTIVE
  Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple-flowered parent of P. hybrida.
  
  
  RESULTS
  We characterized 11 microsatellite loci by screening primers developed using an SSR-enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross-species transferability was tested for other members of the P. integrifolia complex.
  
  
  CONCLUSIONS
  The development of these markers may contribute to population genetics studies in Petunia, and cross-amplification among related species could be a useful tool for research on hybridization and introgression.
authors.affiliation: Molecular Evolution Laboratory, Department of Genetics, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Raquel A
authors.initials: RA
authors.lastname: Kriedt
authors.forename: Aline M C
authors.initials: AM
authors.lastname: Ramos-Fregonezi
authors.forename: Luciano B
authors.initials: LB
authors.lastname: Beheregaray
authors.forename: Sandro L
authors.initials: SL
authors.lastname: Bonatto
authors.forename: Loreta B
authors.initials: LB
authors.lastname: Freitas
mesh_terms: D001938:Brazil; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D032306:Petunia / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D018744:DNA, Plant