title: Molecular detection of human periodontal pathogens in oral swab specimens from dogs in Japan.
abstract: Periodontal diseases are known to be major diseases in humans, and are also common in dogs. The purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. The distribution of an animal gingival organism Porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Capnocytophaga ochracea, Capnocytophaga sputigena, Prevotella intermedia, Prevotella nigrescens, Aggregatibacter actinomycetemcomitans, Campylobacter rectus, and Eikenella corrodens, were evaluated by polymerase chain reaction with species-specific sets of primers. Porphyromonas gulae, Tannerella forsythia and Campylobacter rectus were detected in almost all dogs analyzed, all of which should be regarded as common members of oral flora in dogs. Then, isolation and identification of the Porphyromonas species in swab specimens were performed. There were 35 strains isolated from 22 dogs, and broad-range polymerase chain reaction and sequencing methods revealed that approximately 70% of them were Porphyromonas gulae. In contrast, the frequency of Porphyromonas gingivalis was extremely low. These findings indicate the presence of specific periodontitis-related pathogens in pet dogs, especially Porphyromonas gulae.
authors.affiliation: School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kato
authors.forename: Mitsuyuki
authors.initials: M
authors.lastname: Shirai
authors.forename: Masaru
authors.initials: M
authors.lastname: Murakami
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizusawa
authors.forename: Atsuki
authors.initials: A
authors.lastname: Hagimoto
authors.forename: Koichiro
authors.initials: K
authors.lastname: Wada
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.forename: Takashi
authors.initials: T
authors.lastname: Ooshima
authors.forename: Fumitoshi
authors.initials: F
authors.lastname: Asai
mesh_terms: D000818:Animals; D017931:DNA Primers; D030561:Databases, Nucleic Acid; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D005260:Female; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D007564:Japan; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology; D010510:Periodontal Diseases / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D017861:Porphyromonas / Q000235:genetics / Q000302:isolation & purification; D017421:Sequence Analysis
chemical_list: D017931:DNA Primers