title: Mucosal antibodies induced by tandem repeat of 2F5 epitope block transcytosis of HIV-1.
abstract: Induction of mucosal antibodies to prevent HIV infection is an important strategy for the HIV-1 prophylaxis. Here we report an epitope-vaccine based antigen that was able to elicit mucosal antibodies capable of blocking HIV-1 transcytosis. Because the ELDKWA epitope of neutralizing antibody 2F5 plays a crucial role in transcytosis, a series of immunogens that contain tandem copies of ELDKWA were prepared. Mice were immunized with these immunogens intranasally, and received intraperitoneal+intranasal boosters. The immunogens that contained more ELDKWA epitopes elicited higher level of mucosal ELDKWA-epitope specific IgAs and systemic IgGs. Although the antisera from the immunized mice exhibited mild neutralizing potency to HIV-1 isolates HXB2 and JRFL, the affinity purified mucosal ELDKWA-epitope specific antibodies could block the transcytosis of cell-free CNE3 (a primary isolate of subtype CRF01_AE) in human tight epithelial models.
authors.affiliation: School of Life Sciences, Tsinghua University, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Liling
authors.initials: L
authors.lastname: Xu
authors.forename: Pei
authors.initials: P
authors.lastname: Tong
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Chen
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D007117:Immunization, Secondary / Q000379:methods; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D020080:Tandem Repeat Sequences; D057900:Transcytosis / Q000276:immunology*; D014611:Vaccination / Q000379:methods
chemical_list: D016915:AIDS Vaccines; D057134:Antibodies, Neutralizing; D000939:Epitopes; D015483:HIV Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G