title: Development and validation of an HPLC method for mefloquine hydrochloride determination in tablet dosage form.
abstract: A simple HPLC method for determination of mefloquine hydrochloride in tablets was developed and validated. The separation was carried out on an Xterra RP18 (250 x 4.6 mm id, 5 pm particle size) analytical column. The mobile phase was 0.05 M monobasic potassium phosphate buffer (pH 3.5)-methanol (40 + 60, v/v). The flow rate and wavelength were set to 1 mL/min and 283 nm, respectively. The method was specific for mefloquine hydrochloride in the presence of hydrolytic, oxidative, and photolytic degradation products. It was also linear, precise, accurate, and robust, being suitable for routine QC analyses and stability studies. The developed HPLC method was compared to a previously described spectrophotometric method.
authors.affiliation: Universidade Federal de Minas Gerais, Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Av. Pres. Antônio Carlos 6627, Sala 4029 Bloco 2, 31270-901, Belo Horizonte, MG, Brazil. fhanogueira@gmail.com
authors.forename: Fernando Henrique Andrade
authors.initials: FH
authors.lastname: Nogueira
authors.forename: Letícia de Paula Lana
authors.initials: Lde P
authors.lastname: Goulart
authors.forename: Isabela da Costa
authors.initials: Ida C
authors.lastname: César
authors.forename: Lígia Maria Moreira
authors.initials: LM
authors.lastname: de Campos
authors.forename: Gérson Antônio
authors.initials: GA
authors.lastname: Pianetti
mesh_terms: D000962:Antimalarials / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015767:Mefloquine / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry
chemical_list: D000962:Antimalarials; D013607:Tablets; D015767:Mefloquine