title: Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene codes for 130-kDa toxin protein.
abstract: Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene from Bacillus thuringiensis var. kurstaki borne on pKT230, shuttle vector, was generated. PCR amplification of Cry1Ac gene present in recombinant G. diazotrophicus yielded a 278-bp DNA product. The nitrogenase assay has revealed that the recombinant G. diazotrophicus in sugarcane stem produced similar levels of nitrogenase compared to wild-type G. diazotrophicus. The presence of 130-kDa protein in apoplastic fluid from sugarcane stem harvested from pots inoculated with recombinant G. diazotrophicus shows that the translocated G. diazotrophicus produces 130-kDa protein which is recognized by the hyperimmune antiserum raised against 130-kDa protein. The first instar Eldana saccharina neonate larvae that fed on artificial medium containing recombinant G. diazotrophicus died within 72 h after incubation.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Pondicherry University, India.
authors.forename: M
authors.initials: M
authors.lastname: Subashini
authors.forename: A
authors.initials: A
authors.lastname: Moushumi Priya
authors.forename: B
authors.initials: B
authors.lastname: Sundarakrishnan
authors.forename: S
authors.initials: S
authors.lastname: Jayachandran
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism; D041821:Gluconacetobacter / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D007194:India; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000254:growth & development / Q000382:microbiology; D008855:Microscopy, Electron, Scanning; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D031786:Saccharum / Q000378:metabolism / Q000382:microbiology
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D009591:Nitrogenase