title: Determination of residual fluoroquinolones in honey by liquid chromatography using metal chelate affinity chromatography.
abstract: A new analytical method for the simultaneous determination of seven fluoroquinolones, namely, norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, orbifloxacin, sarafloxacin, and difloxacin, especially in dark-colored honey, has been developed. Fluoroquinolone antibiotics were extracted from samples with MacIlvaine buffer solution (pH 4.0) containing EDTA disodium salt dihydrate. The extracts were treated with both a polymeric cartridge and a metal chelate affinity column preloaded with ferric ion (Fe3+). LC separation with fluorescence detection was performed at 40 degrees C using an Inertsil ODS-4 analytical column (150 x 4.6 mm, 3 microm). The mobile phase was composed of 20 mM/L citrate buffer solution (pH 3.1)-acetonitrile mixture (70 + 30, v/v) containing 1 mM/L sodium dodecyl sulfate. Lomefloxacin was used as an internal standard. The developed method was validated according to the criteria of European Commission Decision 2002/657/EC. Decision limits and detection capabilities were below 2.9 and 4.4 microg/kg, respectively.
authors.affiliation: Japan Food Research Laboratories, 7-4-41, Saitoasagi, Ibaraki, Osaka, 567-0085, Japan. yatsukaway@jfrl.or.jp
authors.forename: Yoh-Ichi
authors.initials: Y
authors.lastname: Yatsukawa
authors.forename: Hironobu
authors.initials: H
authors.lastname: Ito
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsuda
authors.forename: Munetomo
authors.initials: M
authors.lastname: Nakamura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Watai
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujita
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D024841:Fluoroquinolones / Q000737:chemistry*; D006722:Honey / Q000032:analysis*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones