title: Therapeutic effect of frequent injections of GnRH analogue in a beagle with knobbed acrosome abnormality of sperm.
abstract: A Beagle dog (3 years old) that ejaculated high percentages (mean ± SE: 29.1 ± 1.2%) of sperm with a knobbed acrosome abnormality and a low number of sperm and that also had a low plasma testosterone (T) level was given 10 subcutaneous injections of 1 µg of gonadotropin-releasing hormone analogue (GnRH-A) at 3-day intervals. The plasma T level and number of sperm increased 12-14 weeks after the first injection. Although the percentages of sperm with knobbed acrosome abnormality did not change after the GnRH-A therapy, the number of sperm and percentage of actively motile sperm increased after the therapy, and a bitch gave birth to 5 healthy puppies after intravaginal artificial insemination with fresh semen collected 14 weeks after the first injection.
authors.affiliation: Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1–7–1 Kyonancho, Musashino-shi, Tokyo 180–8602, Tokyo, Japan. kawakami@nvlu.ac.jp
authors.forename: Eiichi
authors.initials: E
authors.lastname: Kawakami
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yagi
authors.forename: Masanori
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hori
mesh_terms: D000177:Acrosome / Q000187:drug effects*; D000818:Animals; D000831:Animals, Newborn; D002064:Buserelin / Q000494:pharmacology*; D004285:Dogs / Q000002:abnormalities* / Q000097:blood; D005260:Female; D007279:Injections, Subcutaneous / Q000662:veterinary; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000002:abnormalities*; D013739:Testosterone / Q000097:blood*
chemical_list: D013739:Testosterone; D002064:Buserelin