title: Expression of fur and its antisense α-fur from Microcystis aeruginosa PCC7806 as response to light and oxidative stress.
abstract: Ferric uptake regulation (Fur) proteins are prokaryotic transcriptional regulators that integrate signaling of iron metabolism and oxidative stress responses with several environmental stresses. In photosynthetic organisms, Fur proteins regulate many genes involved in photosynthesis, nitrogen metabolism and other key processes. Also, Fur triggers the expression of virulence factors in many bacterial pathogens, and Fur from Microcystis aeruginosa has been shown to bind promoter regions of the microcystin synthesis gene cluster. In this work, we studied transcriptional responses of fur genes under different light intensities and oxidative stress. An antisense of fur, the α-fur RNA, plays an important role in regulating fur expression under oxidative stress, affecting levels of Fur protein in cells. Importantly, an active photosynthetic electron chain is required for the expression of the fur gene.
authors.affiliation: Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias and BIFI, Universidad de Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martin-Luna
authors.forename: Emma
authors.initials: E
authors.lastname: Sevilla
authors.forename: Andres
authors.initials: A
authors.lastname: Gonzalez
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Bes
authors.forename: Maria F
authors.initials: MF
authors.lastname: Fillat
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Peleato
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015151:Immunoblotting; D008027:Light; D046931:Microcystis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D006861:Hydrogen Peroxide