title: Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 isolate.
abstract: pJIE143 (34 kb), from an Escherichia coli ST131 isolate, carries bla(CTX-M-15) but could not be typed using the standard PCR-based replicon-typing primer set. Complete sequencing revealed a backbone with similarity to IncX plasmids, including a pir-like gene encoding a π-like replication protein and iterons related to those of other IncX plasmids. The 2.971-kb ISEcp1-bla(CTX-M-15)-orf477Δ transposition unit often found within Tn2 is inserted just beyond the end of pir, flanked by 5-bp direct repeats.
authors.affiliation: Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Level 3 ICPMR Building, Sydney, New South Wales 2145, Australia. sally.partridge@swahs.health.nsw.gov.au
authors.forename: Sally R
authors.initials: SR
authors.lastname: Partridge
authors.forename: Justin A
authors.initials: JA
authors.lastname: Ellem
authors.forename: Sasha G
authors.initials: SG
authors.lastname: Tetu
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zong
authors.forename: Ian T
authors.initials: IT
authors.lastname: Paulsen
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Iredell
mesh_terms: D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases