title: Apoptosis of neurons and oligodendrocytes in the spinal cord of spinal hyperostotic mouse (twy/twy): possible pathomechanism of human cervical compressive myelopathy.
abstract: BACKGROUND
  Cervical compressive myelopathy is the most serious complication of cervical spondylosis or ossification of the posterior longitudinal ligament (OPLL) and the most frequent cause of spinal cord dysfunction. There is little information on the exact pathophysiological mechanism responsible for the progressive loss of neural tissue in the spinal cord of such patients. In this study, we used the spinal hyperostotic mouse (twy/twy) as a suitable model of human spondylosis, and OPLL to investigate the cellular and molecular changes in the spinal cord. Mutant twy/twy mouse developed ossification of the ligamentum flavum at C2-C3 and exhibited progressive paralysis.
  
  
  METHODS
  The mutant twy/twy mice, aged 16 and 24 weeks, were used in the present study. The cervical spinal cord was analyzed histologically and immunohistochemically.
  
  
  RESULTS
  We observed that a significant correlation between the proportion of apoptotic oligodendrocytes in the compressed area of the spinal cord and the magnitude of cord compression. Immunohistochemical analysis indicated overexpression of TNFR1, CD95, and p75NTR in the twy/twy mice, which was localized by the immunofluorescence in the neurons and oligodendrocytes.
  
  
  CONCLUSIONS
  The expression of such factors seems to play at least some role in the apoptotic process, which probably contributes to axonal degeneration and demyelination in the twy/twy mice spinal cords with severe compression.
authors.affiliation: Department of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University of Fukui, Matsuoka Shimoaizuki 23-3, Eiheiji, Fukui, 910-1193, Japan. kuchida@u-fukui.ac.jp
authors.forename: Kenzo
authors.initials: K
authors.lastname: Uchida
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakajima
authors.forename: Shuji
authors.initials: S
authors.lastname: Watanabe
authors.forename: Takafumi
authors.initials: T
authors.lastname: Yayama
authors.forename: Alexander Rodriguez
authors.initials: AR
authors.lastname: Guerrero
authors.forename: Tomoo
authors.initials: T
authors.lastname: Inukai
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hirai
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sugita
authors.forename: William E
authors.initials: WE
authors.lastname: Johnson
authors.forename: Hisatoshi
authors.initials: H
authors.lastname: Baba
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D015576:Hyperostosis / Q000150:complications / Q000235:genetics / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008817:Mice, Mutant Strains; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D009836:Oligodendroglia / Q000378:metabolism / Q000473:pathology*; D013116:Spinal Cord / Q000473:pathology*; D013117:Spinal Cord Compression / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D055009:Spondylosis / Q000150:complications* / Q000235:genetics / Q000473:pathology