title: [A comparison of the myogenic characteristics between stem cell antigen-(1+) and stem cell antigen-(1-) cells derived from skeletal muscle].
abstract: OBJECTIVE
  To probe into the function of stem cell antigen-1 (Sca-1) in cell proliferation and differentiation.
  
  
  METHODS
  Sca-(1+) and Sca-(1-) muscle derived cells were separated from C57BU6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca(-1), MyoD and Myogenin expression in cultured Sca(-1+) and Sca-(1-) muscle derived cells.
  
  
  RESULTS
  The difference of the proliferative curve of Sca-(1+) and Sca-(1-) muscle derived cells cultured 3 days in vitro was not apparent, but Sca-(1-) muscle derived cells had a accelerated division rate in the follow days compared to the Sca-(1+) muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-(1+) than Sca-(1-) muscle derived cells.
  
  
  CONCLUSIONS
  Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.
authors.affiliation: Dept. of Orthodontics, School of Stomatology, Capital Medical University, Beijing 100050, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Che
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhu
authors.forename: Jie
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D000950:Antigens, Ly; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D005434:Flow Cytometry; D008565:Membrane Proteins; D051379:Mice; D018482:Muscle, Skeletal; D018008:Myogenin; D013234:Stem Cells
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D018008:Myogenin