title: Electrokinetic DNA transport in 20 nm-high nanoslits: evidence for movement through a wall-adsorbed.
abstract: The electrokinetic transport behavior of λ-DNA (48 kbp) in 20 nm-high fused-silica nanoslits in the presence of short-chain PVP is investigated. Mobility and video data show a number of phenomena that are typical of DNA transport through gels or polymer solutions, thus indicative of rigid migration obstacles in the DNA pathway. Calculations show that a several nanometer thin layer of wall-adsorbed PVP ('nano-gel') can provide such a rigid obstacle matrix to the DNA. Such ultrathin wall-adsorbed polymer layers represent a new type of matrix for electrokinetic DNA separation.
authors.affiliation: Nanobioengineering Group, Institute for Bioengineering of Catalonia, Josep Samitier, Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Castillo-Fernandez
authors.forename: Georgette B
authors.initials: GB
authors.lastname: Salieb-Beugelaar
authors.forename: Jan W
authors.initials: JW
authors.lastname: van Nieuwkasteele
authors.forename: Johan G
authors.initials: JG
authors.lastname: Bomer
authors.forename: Martin
authors.initials: M
authors.lastname: Arundell
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier
authors.forename: Albert
authors.initials: A
authors.lastname: van den Berg
authors.forename: Jan C T
authors.initials: JC
authors.lastname: Eijkel
mesh_terms: D000327:Adsorption; D010582:Bacteriophage lambda / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry*; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011108:Polymers; D012822:Silicon Dioxide / Q000737:chemistry; D018709:Statistics, Nonparametric
chemical_list: D004279:DNA, Viral; D011108:Polymers; D012822:Silicon Dioxide