title: [Expression of periodontal ligament-associated protein-1 in normal periodontal tissues and cells in rat].
abstract: OBJECTIVE
  To examine the expression of periodontal ligament-associated protein-1 (PLAP-1) in the periodontal tissues and periodontal ligament cells (PDLC).
  
  
  METHODS
  The PLAP-1 expression in normal periodontal tissue was examined by immunohistochemistry. The protein expression and mRNA transcription of PLAP-1 in PDLC were investigated by immunocytochemistry and reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  PLAP-1 was expressed in periodontium but not in cementum, alveolar bone and gingival tissues. PLAP-1 expression was observed in cell plasma, but not in nuclei. There was a 350 bp electrophoresis band representing PLAP-1 mRNA.
  
  
  CONCLUSIONS
  PLAP-1 may play a role in physiology of periodontal tissues and cells in normal adult rats.
authors.affiliation: Department of Periodontology, School of Stomatology, Shandong University, Jinan, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Pi-Shan
authors.initials: PS
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Chao
authors.initials: C
authors.lastname: Hou
mesh_terms: D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D010519:Periodontium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger