title: Azide-reactive liposome for chemoselective and biocompatible liposomal surface functionalization and glyco-liposomal microarray fabrication.
abstract: Chemically selective liposomal surface functionalization and liposomal microarray fabrication using azide-reactive liposomes are described. First, liposome carrying PEG-triphenylphosphine was prepared for Staudinger ligation with azide-containing biotin, which was conducted in PBS buffer (pH 7.4) at room temperature without a catalyst. Then, immobilization and microarray fabrication of the biotinylated liposome onto a streptavidin-modified glass slide via the specific streptavidin/biotin interaction were investigated by comparing with directly formed biotin-liposome, which was prepared by the conventional liposome formulation of lipid-biotin with all other lipid components. Next, the covalent microarray fabrication of liposome carrying triphenylphosphine onto an azide-modified glass slide and its further glyco-modification with azide-containing carbohydrate were demonstrated for glyco-liposomal microarray fabrication via Staudinger ligation. Fluorescence imaging confirmed the successful immobilization and protein binding of the intact immobilized liposomes and arrayed glyco-liposomes. The azide-reactive liposome provides a facile strategy for membrane-mimetic glyco-array fabrication, which may find important biological and biomedical applications such as studying carbohydrate-protein interactions and toxin and antibody screening.
authors.affiliation: Department of Chemistry, Cleveland State University, Cleveland, Ohio 44115, United States.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ma
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Valentinas
authors.initials: V
authors.lastname: Gruzdys
authors.forename: Xue-Long
authors.initials: XL
authors.lastname: Sun
mesh_terms: D001386:Azides / Q000737:chemistry*; D040761:Biomimetic Materials / Q000737:chemistry; D019921:Biotinylation; D002241:Carbohydrates / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005898:Glass / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods*; D055616:Microtechnology / Q000379:methods*; D009943:Organophosphorus Compounds / Q000737:chemistry; D011327:Printing; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D001386:Azides; D002241:Carbohydrates; D020099:Coated Materials, Biocompatible; D008081:Liposomes; D009943:Organophosphorus Compounds; C061896:triphenylphosphine