title: The effect of plasma removal from apheresis platelet concentrates on platelet function.
abstract: The effect of plasma removal on platelet function has scarcely been investigated. Plasma removal from apheresis platelet concentrates was achieved by centrifugation at 5000 g for 6 min or 2000 g for 10 min. After resting for 1 h, platelet concentrates were resuspended in 0·9% NaCl. Platelet function was tested before centrifugation and after resuspension by multiple electrode impedance aggregometry (MEA) and light transmission aggregometry (LTA). Plasma removal resulted in 10-14% lower response to TRAP-6 by MEA using both washing procedures, whereas TRAP-6-inducible aggregation by LTA increased slightly (2-5%). Neither plasma removal method affected collagen-induced aggregation. Thus, platelet function did not deteriorate significantly by either method.
authors.affiliation: Clinical Department of Blood Group Serology and Transfusion Medicine, Medical University Vienna, Vienna, Austria. petra.jilma@meduniwien.ac.at
authors.forename: P
authors.initials: P
authors.lastname: Jilma-Stohlawetz
authors.forename: B
authors.initials: B
authors.lastname: Eichelberger
authors.forename: J
authors.initials: J
authors.lastname: List
authors.forename: B
authors.initials: B
authors.lastname: Jilma
authors.forename: S
authors.initials: S
authors.lastname: Panzer
authors.forename: G
authors.initials: G
authors.lastname: Leitner
mesh_terms: D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma; D015539:Platelet Activation; D010979:Platelet Function Tests; D010983:Plateletpheresis / Q000379:methods*