title: Regulation of the BRCA1 gene by an SRC3/53BP1 complex.
abstract: BACKGROUND
  Steroid Receptor coactivator 3(SRC3) is an oncogene and a member of the SRC family of nuclear receptor coactivator proteins that mediate the transcriptional effects of nuclear hormone receptors as well as other transcription factors.
  
  
  RESULTS
  We have used protein purification and mass spectrometry to identify the 53BP1 tumour suppressor as a novel SRC3-associated protein. Copurification was demonstrated using multiple antibodies, and was not dependent on DNA damage suggesting that SRC3 is not directly involved in the DNA damage response. However using chromatin immunoprecipitation(ChIP) and siRNA knockdown, we have demonstrated that both SRC3 and 53BP1 co-occupy the same region of the BRCA1 promoter and both are required for BRCA1 expression in HeLa cells.
  
  
  CONCLUSIONS
  Our results suggest that both 53BP1 and SRC3 have a common function that converge at the BRCA1 promoter and possibly other genes important for DNA repair and genomic stability.
authors.affiliation: Department of Oncology, London Regional Cancer Program and Lawson Health Research Institute, 790 Commissioners Rd, London, Ontario N6A 4L6 Canada.
authors.forename: Dale
authors.initials: D
authors.lastname: Corkery
authors.forename: Gobi
authors.initials: G
authors.lastname: Thillainadesan
authors.forename: Niamh
authors.initials: N
authors.lastname: Coughlan
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Mohan
authors.forename: Majdina
authors.initials: M
authors.lastname: Isovic
authors.forename: Marc
authors.initials: M
authors.lastname: Tini
authors.forename: Joseph
authors.initials: J
authors.lastname: Torchia
mesh_terms: D050882:CREB-Binding Protein / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D004249:DNA Damage; D055785:Gene Knockdown Techniques; D019398:Genes, BRCA1; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013058:Mass Spectrometry; D056921:Nuclear Receptor Coactivator 3 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000235:genetics; D000071857:Tumor Suppressor p53-Binding Protein 1
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; C498301:TP53BP1 protein, human; D000071857:Tumor Suppressor p53-Binding Protein 1; D050882:CREB-Binding Protein; C538866:NCOA3 protein, human; D056921:Nuclear Receptor Coactivator 3