title: Radiological and histopathological evaluation of experimentally-induced periapical lesion in rats.
abstract: OBJECTIVE
  This study evaluated experimentally-induced periapical bone loss sites using digital radiographic and histopathologic parameters.
  
  
  METHODS
  Twenty-seven Wistar rats were submitted to coronal opening of their mandibular right first molars. They were radiographed at 2, 15 and 30 days after the operative procedure by two digital radiographic storage phosphor plates (Digora®). The images were analyzed by creating a region of interest at the periapical region of each tooth (ImageJ) and registering the corresponding pixel values. After the sacrifice, the specimens were submitted to microscopic analysis in order to confirm the pulpal and periapical status of the tooth.
  
  
  RESULTS
  There was significant statistically difference between the control and test sides in all the experimental periods regarding the pixel values (two-way ANOVA; p<0.05).
  
  
  CONCLUSIONS
  The microscopic analysis proved that a periapical disease development occurred during the experimental periods with an evolution from pulpal necrosis to periapical bone resorption.
authors.affiliation: Department of Stomatology, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Renata Cordeiro
authors.initials: RC
authors.lastname: Teixeira
authors.forename: Cassia Maria Fischer
authors.initials: CM
authors.lastname: Rubira
authors.forename: Gerson Francisco
authors.initials: GF
authors.lastname: Assis
authors.forename: José Roberto Pereira
authors.initials: JR
authors.lastname: Lauris
authors.forename: Tania Mary
authors.initials: TM
authors.lastname: Cestari
authors.forename: Izabel Regina Fischer
authors.initials: IR
authors.lastname: Rubira-Bullen
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000000981:diagnostic imaging / Q000473:pathology; D003790:Dental Pulp Necrosis / Q000000981:diagnostic imaging / Q000473:pathology; D010483:Periapical Diseases / Q000000981:diagnostic imaging / Q000473:pathology; D011856:Radiographic Image Enhancement; D019252:Radiography, Dental, Digital / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors