title: A genetic association between ß3-aderenoceptor and cholinergic receptor muscarinic 3 polymorphisms in irritable bowel syndrome.
abstract: OBJECTIVE
  We examined the association of ß3-adrenoceptor (ß3-AR) and cholinergic receptor muscarinic 3 (CHRM3) polymorphisms with irritable bowel syndrome (IBS).
  
  
  METHODS
  DNA was obtained from 81 IBS patients (39 diarrhea type, IBS-D; 25 constipation type, IBS-C, 5 mixed type, IBS-M; and 12 unsubtyped, IBS-U) and 73 controls. For the analyses IBS-(M + U) was combined into one group (NonDNonC). ß3-AR and CHRM3 polymorphisms were determined by the polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method.
  
  
  RESULTS
  The ß3-AR genotype frequencies of T/C in IBS patients were significantly higher compared to those in controls. The distribution of the CHRM3 genotypes, C/C, T/C and T/T, was not significantly different between IBS patients and controls. The distribution of the ß3-AR and CHRM3 genotypes was not significantly different between IBS-D, IBS-C and NonDNonC. The frequencies of the CHRM3 genotypes in IBS were significantly different between disease duration greater than and less than 3 years.
  
  
  CONCLUSIONS
  ß3-AR polymorphisms in IBS patients are different compared to controls, and CHRM3 polymorphisms are also likely associated with disease duration in IBS. ß3-AR and CHRM3 polymorphisms could be associated with IBS.
authors.affiliation: Department of Internal Medicine, Iwate Medical University, Iwate, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Onodera
authors.forename: Toshimi
authors.initials: T
authors.lastname: Chiba
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Sugai
authors.forename: Wataru
authors.initials: W
authors.lastname: Habano
mesh_terms: D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D015897:Comorbidity; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D043183:Irritable Bowel Syndrome / Q000235:genetics*; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D043587:Receptor, Muscarinic M3 / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*
chemical_list: D043587:Receptor, Muscarinic M3; D022702:Receptors, Adrenergic, beta-3