title: Biological activity of camel milk casein following enzymatic digestion.
abstract: The aim of this study was to investigate the effects of enzymatic hydrolysis with digestive enzymes of camel whole casein and beta-casein (β-CN) on their antioxidant and Angiotensin Converting Enzyme (ACE)-inhibitory properties. Peptides in each hydrolysate were fractionated with ultra-filtration membranes. The antioxidant activity was determined using a Trolox equivalent antioxidant capacity (TEAC) scale. After enzymatic hydrolysis, both antioxidant and ACE-inhibitory activities of camel whole casein and camel β-CN were enhanced. Camel whole casein and β-CN showed significant ACE-inhibitory activities after hydrolysis with pepsin alone and after pepsinolysis followed by trypsinolysis and chymotrypsinolysis. Camel β-CN showed high antioxidant activity after hydrolysis with chymotrypsin. The results of this study suggest that when camel milk is consumed and digested, the produced peptides start to act as natural antioxidants and ACE-inhibitors.
authors.affiliation: Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Salami
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
authors.forename: Faezeh
authors.initials: F
authors.lastname: Moosavi-Movahedi
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ehsani
authors.forename: Reza
authors.initials: R
authors.lastname: Yousefi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farhadi
authors.forename: Amir
authors.initials: A
authors.lastname: Niasari-Naslaji
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboury
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002162:Camelus; D002364:Caseins / Q000378:metabolism* / Q000494:pharmacology*; D002918:Chymotrypsin / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D008892:Milk / Q000737:chemistry*; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D002364:Caseins; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A