title: Influence of doxorubicin on fluconazole susceptibility and efflux pump gene expression of Candida dubliniensis.
abstract: The effect of doxorubicin (DOX) on the fluconazole (FLU) susceptibility of C. dubliniensis was investigated. Isolates were exposed to DOX and FLU in a chequerboard assay and resistance gene expressions were analysed after DOX exposure. The susceptibility of the yeast to FLU was decreased in the presence of DOX in the chequerboard assay with FIC indices suggesting an antagonistic effect. Gene expression analyses showed an overexpression of CdCDR2. Hence, DOX was found to have an impact on resistance mechanisms in C. dubliniensis isolates.
authors.affiliation: Charité-Universitätsmedizin Berlin, Charit Campus Mitte, Medical Department, Division of Oncology and Haematology, Berlin, Germany. bettina_schulz@arcor.de
authors.forename: Bettina
authors.initials: B
authors.lastname: Schulz
authors.forename: Mathias
authors.initials: M
authors.lastname: Knobloch
authors.forename: Gary P
authors.initials: GP
authors.lastname: Moran
authors.forename: Kai
authors.initials: K
authors.lastname: Weber
authors.forename: Markus
authors.initials: M
authors.lastname: Ruhnke
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects*; D002175:Candida / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D015725:Fluconazole / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents; D004317:Doxorubicin; D015725:Fluconazole