title: The yeast prion protein Ure2: insights into the mechanism of amyloid formation.
abstract: Ure2, a regulator of nitrogen metabolism, is the protein determinant of the [URE3] prion state in Saccharomyces cerevisiae. Upon conversion into the prion form, Ure2 undergoes a heritable conformational change to an amyloid-like aggregated state and loses its regulatory function. A number of molecular chaperones have been found to affect the prion properties of Ure2. The studies carried out in our laboratory have been aimed at elucidating the structure of Ure2 fibrils, the mechanism of amyloid formation and the effect of chaperones on the fibril formation of Ure2.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Chen
authors.forename: Elizabeth B
authors.initials: EB
authors.lastname: Sawyer
authors.forename: Sarah
authors.initials: S
authors.lastname: Perrett
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000096:biosynthesis* / Q000648:ultrastructure; D000818:Animals; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D011328:Prions / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000682:Amyloid; D018832:Molecular Chaperones; D011328:Prions; D029701:Saccharomyces cerevisiae Proteins; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae