title: Antioxidant capacity and larvicidal and antifungal activities of essential oils and extracts from Piper krukoffii.
abstract: The leaves and twigs of Piper krukoffii, collected in the Carajás National Forest, north Brazil, yielded essential oils (2.0% and 0.8%), the main constituents of which were myristicin (40.3% and 26.7%), apiole (25.4% and 34.1%) and elemicin (2.8% and 3.0%). The antioxidant activities of the oils, methanol extract and its sub-fractions were evaluated. The DPPH EC50 values varied from the ethyl acetate sub-fraction (73.4 +/- 3.7 microg/mL) to the methanol extract (24.9 +/- 0.8 microg/mL), and the ABTS TEAC values ranged in the same order from 265.7 to 349.2 microMol TE/g. These results indicated a significant antioxidant activity for the plant. The lignans (-)-kusunokin, yatein, (-)-hinokin and cubebin were identified in the methanol extract. The hydro-methanolic sub-fraction showed a high value for total phenol content (106.5 +/- 0.7 mg GAE/g), as well as 1H NMR signals for sugar moieties. Crude extracts and sub-fractions were also able to inhibit beta-carotene bleaching, varying from 22.4 to 47.1%. The oils from the leaves and twigs showed strong larvicidal (21.4 and 3.6 microg/mL) and fungicide (0.5 and 0.1 microg/mL) activities.
authors.affiliation: Programa de Pós-Graduação em Química, Universidade Federal do Pará, 66075-900 Belém, PA, Brazil.
authors.forename: Joyce Kelly R
authors.initials: JK
authors.lastname: da Silva
authors.forename: Eloisa Helena A
authors.initials: EH
authors.lastname: Andrade
authors.forename: Massuo J
authors.initials: MJ
authors.lastname: Kato
authors.forename: Léa Maria M
authors.initials: LM
authors.lastname: Carreira
authors.forename: Elsie F
authors.initials: EF
authors.lastname: Guimarães
authors.forename: José Guilherme S
authors.initials: JG
authors.lastname: Maia
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001156:Artemia / Q000187:drug effects; D001713:Biphenyl Compounds; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010851:Picrates; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000935:Antifungal Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D007306:Insecticides; D009822:Oils, Volatile; D010851:Picrates; D010936:Plant Extracts; D010938:Plant Oils; D019207:beta Carotene; C004931:1,1-diphenyl-2-picrylhydrazyl