title: Quantitative analysis of transcript variants of CHM gene containing LTR12C element in humans.
abstract: Choroideremia (CHM) is essential for the posttranslational activation of retina-specific Rab protein. Transcript variants (a and b) of the CHM gene were detected in human cancer cells and tissues. Sequence analysis of the both variants found that isoform b is caused by an LTR12C element offering an alternative splicing site within the CHM gene. We performed real-time RT-PCR analysis to study expression levels of the CHM transcript variants in tumor and adjacent normal tissue samples. Our results showed that CHM isoform b was highly expressed in tumor tissues but its expression levels were relatively low in those of adjacent normal tissues including colon, testis, and lung. In addition, high expression levels of CHM isoform b were detected in the cancer cell lines of colon and lung, and colon cancer patient tissues. Thus, we suggest that expression levels of alternative transcripts of the CHM gene could be used as a molecular marker system to identify human cancers.
authors.affiliation: Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Yi-Deun
authors.initials: YD
authors.lastname: Jung
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Huh
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Kim
authors.forename: Yun-Ji
authors.initials: YJ
authors.lastname: Kim
authors.forename: Kung
authors.initials: K
authors.lastname: Ahn
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Ha
authors.forename: Ja-Rang
authors.initials: JR
authors.lastname: Lee
authors.forename: Joo Mi
authors.initials: JM
authors.lastname: Yi
authors.forename: Jae-Woo
authors.initials: JW
authors.lastname: Moon
authors.forename: Tae-Oh
authors.initials: TO
authors.lastname: Kim
authors.forename: Geun-Am
authors.initials: GA
authors.lastname: Song
authors.forename: Kyudong
authors.initials: K
authors.lastname: Han
authors.forename: Heui-Soo
authors.initials: HS
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D017398:Alternative Splicing; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D015794:Choroideremia / Q000235:genetics; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000175:diagnosis*; D020033:Protein Isoforms / Q000235:genetics; D013737:Testis / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C081787:CHM protein, human; D020033:Protein Isoforms