title: Expression and function of miR-27b in human glioma.
abstract: Our previous miRNAs profiling study showed that miR-27b was up-regulated             in glioma cells compared with H4 low grade astrocytoma cells. However, the main             function of miR-27b in glioma in not known yet. The aim of this study was to investigate             the expression and function of miR-27b in the pathogenesis of glioma. Real-time             PCR showed that miR-27b was up-regulated in glioma samples and glioma cells. Down-regulation             of miR-27b triggered growth inhibition, induced apoptosis and inhibited invasion             in glioma cells. Furthermore, TOPflash luciferase activity was decreased significantly,             while FOPflash luciferase did not change significantly. In addition, Western blot             assay showed that STAT3, c-myc and cyclin D1 were knocked down after treatment             with miR-27b inhibitor. These findings suggest that aberrantly up-regulated miR-27b             may be one of the critical factors that contribute to malignancy in human gliomas.
authors.affiliation: Department of Neurosurgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, PR China.
authors.forename: Lingchao
authors.initials: L
authors.lastname: Chen
authors.forename: Huibing
authors.initials: H
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Kailiang
authors.initials: K
authors.lastname: Zhang
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Wang
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Liu
authors.forename: Yongri
authors.initials: Y
authors.lastname: Zheng
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Peiyu
authors.initials: P
authors.lastname: Pu
authors.forename: Chuanlu
authors.initials: C
authors.lastname: Jiang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Kang
mesh_terms: D017209:Apoptosis; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D017930:Genes, Reporter; D005910:Glioma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation; D051176:beta Catenin / Q000378:metabolism
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C530104:CCND1 protein, human; C531388:MIRN27 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D016271:Proto-Oncogene Proteins c-myc; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051176:beta Catenin; D019938:Cyclin D1; D049410:Luciferases, Renilla