title: In vitro anti Mycobacterium tuberculosis H37Rv activity of Lannea acida A. Rich from Burkina Faso.
abstract: The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 microg mL(-1)) and showed only a slight modification of lactate dehydrogenase (LDH) release. The rate of monocytes in different stages of mitosis had been amended in absence and presence of extract as follows: Go/G1 58.83-59.83%; synthesis 21.95-18.64%; mitosis 16.67-15.77%; necrosis 2.65-5.64%. The percentage of inhibition of Mycobacterium tuberculosis proliferation was respectively 77.6 and 36.8% at 1.2 and 0.6 mg mL(-1) of extract. This is an interesting experimental study on antimicrobial and immune-stimulating properties of Lannea acida ethanol-water (70% v/v) extract which may contain potential antibacterial and immune-stimulating agents for clinical use.
authors.affiliation: CRSBAN, Laboratoire de Biochimie et Pharmacologie, Université de Ouagadougou, 03 BP 7131 Ouagadougou Burkina Faso.
authors.forename: L
authors.initials: L
authors.lastname: Ouattara
authors.forename: J
authors.initials: J
authors.lastname: Koudou
authors.forename: D S
authors.initials: DS
authors.lastname: Karou
authors.forename: L
authors.initials: L
authors.lastname: Giacò
authors.forename: G
authors.initials: G
authors.lastname: Capelli
authors.forename: J
authors.initials: J
authors.lastname: Simpore
authors.forename: M
authors.initials: M
authors.lastname: Fraziano
authors.forename: V
authors.initials: V
authors.lastname: Colizzi
authors.forename: A S
authors.initials: AS
authors.lastname: Traore
mesh_terms: D027926:Anacardiaceae / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002050:Burkina Faso; D002453:Cell Cycle; D002460:Cell Line; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008264:Macrophages / Q000382:microbiology; D008938:Mitosis; D009000:Monocytes / Q000382:microbiology; D009164:Mycobacterium Infections / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009336:Necrosis; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D018950:Lipopolysaccharide Receptors; D014867:Water; D000431:Ethanol; D007770:L-Lactate Dehydrogenase