title: Electrochemical and optical sensing of reactive oxygen species: pathway to an integrated intracellular and extracellular measurement platform.
abstract: A comprehensive understanding of ROS (reactive oxygen species)-dependent cellular interaction requires the previously unmet ability to simultaneously monitor the intra- and extra-cellular environments. The present review assesses the potential of novel electrochemical and fluorescent-based nanosensor approaches to address the limitations of existing techniques for ROS analysis. Data generated by these new approaches have already contributed significantly to current understanding of the roles that these species play in various in vitro scenarios. However, integration of these novel approaches has the potential to offer, for the first time, the unparalleled ability to measure simultaneously and in real-time ROS flux in both the intra- and extra-cellular environments.
authors.affiliation: Diagnostic and Therapeutic Technologies, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK. philip.manning@ncl.ac.uk
authors.forename: Philip
authors.initials: P
authors.lastname: Manning
authors.forename: Calum J
authors.initials: CJ
authors.lastname: McNeil
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D049329:Nanostructures; D055096:Optical Devices; D017382:Reactive Oxygen Species / Q000032:analysis*
chemical_list: D017382:Reactive Oxygen Species