title: Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation.
abstract: Quantitative polymerase chain reaction (Q-PCR) allows for the accurate and reproducible determination of the amount of target DNA in a sample through the measurement of PCR product accumulation in "real time." This method determines starting target DNA quantity over a large assay dynamic range and requires no post-PCR sample manipulation. When used in combination with the method of chromatin immunoprecipitation (ChIP), the amount of protein binding to a specific region of DNA can be accurately and rapidly determined. A method for quantifying the presence of acetylated histones H3 and H4 on different regions of a target locus using Q-PCR after ChIP is described.
authors.affiliation: Department of Urology, University of Southern California, Los Angeles, CA, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Irvine
authors.forename: Cindy
authors.initials: C
authors.lastname: Okitsu
authors.forename: Chih-Lin
authors.initials: CL
authors.lastname: Hsieh
mesh_terms: D000107:Acetylation; D000818:Animals; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D002843:Chromatin