title: Plasmid containing CpG motifs enhances the efficacy of porcine reproductive and respiratory syndrome live attenuated vaccine.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is now among the most important swine diseases that affect the Chinese swine industry. Both killed and live attenuated vaccines are currently used against the disease, but neither of them could provide full protection after vaccination. In the present study, the adjuvanticity of a plasmid containing CpG motifs (pUC18-CpG) was introduced to enhance the efficacy of a commercial PRRS live attenuated vaccine. After vaccination, PRRSV-specific antibodies, PRRSV-specific cytokines, and clinical parameters were studied and compared between different vaccinated groups. During a following challenge study, co-administration of pUC18-CpG with the vaccine could confer higher protection rate. Our results have shown that co-administration of pUC18-CpG with the vaccine could elicit more potent adaptive immune response and provide better protection.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Guo
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Hou
authors.forename: Guoqin
authors.initials: G
authors.lastname: Zhai
authors.forename: Hongfei
authors.initials: H
authors.lastname: Zhu
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Vizcaíno
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D018899:CpG Islands / Q000235:genetics* / Q000276:immunology; D016207:Cytokines / Q000097:blood; D056724:Immunity, Humoral / Q000276:immunology; D010957:Plasmids / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use; D019318:Porcine Reproductive and Respiratory Syndrome / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine / Q000276:immunology / Q000821:virology; D014613:Vaccines, Attenuated / Q000276:immunology / Q000627:therapeutic use; D014765:Viral Vaccines / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D016207:Cytokines; D014613:Vaccines, Attenuated; D014765:Viral Vaccines