title: Generic microtiter plate assay for triaging clinical specimens prior to genotyping of human papillomavirus DNA via consensus PCR.
abstract: A generic human papillomavirus (HPV) probe assay was compared to the Linear Array to detect HPV DNA in 1,013 clinical specimens. The sensitivity, specificity, and negative predictive value of the assay were 99.5% (95% confidence interval [CI], 98.4% to 99.9%), 58.6% (95% CI, 53.9% to 63.1%), and 98.9% (95% CI, 96.5% to 99.8%), respectively. This assay conveniently identifies HPV-positive specimens.
authors.affiliation: Laboratoire de Virologie Moléculaire, Centre de Recherche, Département de Microbiologie-Infectiologie et de Gynécologie-Obstétrique, Centre Hospitalier de l’Université de Montréal, Montréal, Québec, Canada.
authors.forename: Véronique
authors.initials: V
authors.lastname: Legault
authors.forename: Ann
authors.initials: A
authors.lastname: Burchell
authors.forename: Patricia
authors.initials: P
authors.lastname: Goggin
authors.forename: Belinda
authors.initials: B
authors.lastname: Nicolau
authors.forename: Paul
authors.initials: P
authors.lastname: Brassard
authors.forename: Julie
authors.initials: J
authors.lastname: Guenoun
authors.forename: Pierre
authors.initials: P
authors.lastname: Forest
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Mayrand
authors.forename: Eduardo Luis
authors.initials: EL
authors.lastname: Franco
authors.forename: Francois
authors.initials: F
authors.lastname: Coutlée
mesh_terms: D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*
chemical_list: D004279:DNA, Viral