title: In vitro activity of isavuconazole against 208 Aspergillus flavus isolates in comparison with 7 other antifungal agents: assessment according to the methodology of the European Committee on Antimicrobial Susceptibility Testing.
abstract: Aspergillus flavus is the second most common species causing invasive aspergillosis after A. fumigatus. In certain countries like India, Sudan, and Saudi Arabia, A. flavus is most frequently isolated from patients with fungal rhinosinusitis and endophthalmitis. A. flavus exhibit an increased resistance to antifungal agents compared to A. fumigatus. We determined the in vitro activity of isavuconazole, voriconazole, posaconazole, itraconazole, amphotericin B, caspofungin, micafungin, and anidulafungin against 208 isolates of A. flavus by the EUCAST method and compared with the results obtained by the CLSI method. Isavuconazole and voriconazole MICs were ≤2 μg/mL in 99% and 95%, respectively. Posaconazole and itraconazole MICs were ≤0.5 and ≤1 μg/mL, respectively, for all isolates. MICs of amphotericin B were ≥2 μg/mL in 91%; 36% of them exhibited MICs of ≥8 μg/mL. All echinocandins demonstrated good anti-A. flavus activity. The essential agreement of the MIC/MEC results by EUCAST with CLSI broth dilution method assessed at ±2 dilutions was good for itraconazole (97.8%), voriconazole (100%), posaconazole (98.3%), isavuconazole (98.9%), caspofungin (99.4%), and anidulafungin (100%), but poor for amphotericin B (53.5%) and micafungin (79.1%).
authors.affiliation: Mycology Division, Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: Shivaprakash M
authors.initials: SM
authors.lastname: Rudramurthy
authors.forename: Arunaloke
authors.initials: A
authors.lastname: Chakrabarti
authors.forename: Erik
authors.initials: E
authors.lastname: Geertsen
authors.forename: Johan W
authors.initials: JW
authors.lastname: Mouton
authors.forename: Jacques F
authors.initials: JF
authors.lastname: Meis
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology; D001231:Aspergillus flavus / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D007194:India; D008826:Microbial Sensitivity Tests; D009426:Netherlands; D009570:Nitriles / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D009570:Nitriles; D011725:Pyridines; D014230:Triazoles; C508735:isavuconazole