title: Neisseria gonorrhoeae NspA induces specific bactericidal and opsonic antibodies in mice.
abstract: Neisseria gonorrhoeae surface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene from N. gonorrhoeae strain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) indicated that all immunized mice generated measurable NspA-specific IgG and IgA in serum and secretory IgA (sIgA) in vaginal wash fluids. The antisera had bactericidal and opsonic activities. These data demonstrated that NspA induced antibodies with antigonococcal activity.
authors.affiliation: Department of Pathogen Biology and Immunology, Yangzhou University, College of Medicine, Yangzhou 225001, Jiangsu Province, China. gcli@yzu.edu.cn
authors.forename: Guocai
authors.initials: G
authors.lastname: Li
authors.forename: Hongmei
authors.initials: H
authors.lastname: Jiao
authors.forename: Guihua
authors.initials: G
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Litian
authors.initials: L
authors.lastname: Zhu
authors.forename: Rushan
authors.initials: R
authors.lastname: Xie
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.forename: Hongju
authors.initials: H
authors.lastname: Chen
authors.forename: Mingchun
authors.initials: M
authors.lastname: Ji
mesh_terms: D000281:Administration, Intranasal; D000282:Administration, Intravaginal; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods; D007117:Immunization, Secondary / Q000379:methods; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D009895:Opsonin Proteins / Q000097:blood*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014621:Vagina / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C105506:NspA protein, Neisseria; D009895:Opsonin Proteins; D019444:Vaccines, DNA