title: The crystal structure of dynamin.
abstract: Dynamin-related proteins (DRPs) are multi-domain GTPases that function via oligomerization and GTP-dependent conformational changes to play central roles in regulating membrane structure across phylogenetic kingdoms. How DRPs harness self-assembly and GTP-dependent conformational changes to remodel membranes is not understood. Here we present the crystal structure of an assembly-deficient mammalian endocytic DRP, dynamin 1, lacking the proline-rich domain, in its nucleotide-free state. The dynamin 1 monomer is an extended structure with the GTPase domain and bundle signalling element positioned on top of a long helical stalk with the pleckstrin homology domain flexibly attached on its opposing end. Dynamin 1 dimer and higher order dimer multimers form via interfaces located in the stalk. Analysis of these interfaces provides insight into DRP family member specificity and regulation and provides a framework for understanding the biogenesis of higher order DRP structures and the mechanism of DRP-mediated membrane scission events.
authors.affiliation: Department of Molecular and Cellular Biology, University of California, Davis, Davis, California 95616, USA.
authors.forename: Marijn G J
authors.initials: MG
authors.lastname: Ford
authors.forename: Simon
authors.initials: S
authors.lastname: Jenni
authors.forename: Jodi
authors.initials: J
authors.lastname: Nunnari
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D034284:Dynamin I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009711:Nucleotides; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000235:genetics; D051381:Rats
chemical_list: D009711:Nucleotides; D006160:Guanosine Triphosphate; D034284:Dynamin I