title: Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells.
abstract: Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E(2) (PGE(2)) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE(2)-producing enzymes, cyclooxygenase-2 and microsomal PGE(2) synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-κB) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-κB pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.
authors.affiliation: Department of Periodontology, School of Dentistry, Showa University Dental Hospital, Tokyo, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Nagahama
authors.forename: Takashi
authors.initials: T
authors.lastname: Obama
authors.forename: Michihiko
authors.initials: M
authors.lastname: Usui
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kanazawa
authors.forename: Sanju
authors.initials: S
authors.lastname: Iwamoto
authors.forename: Kazushige
authors.initials: K
authors.lastname: Suzuki
authors.forename: Akira
authors.initials: A
authors.lastname: Miyazaki
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Matsuo
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itabe
mesh_terms: D018955:CD36 Antigens / Q000096:biosynthesis; D002460:Cell Line; D055113:Chronic Periodontitis / Q000139:chemically induced / Q000201:enzymology*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis; D005881:Gingiva / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology; D008077:Lipoproteins, LDL / Q000378:metabolism* / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D009061:Mouth Mucosa / Q000187:drug effects / Q000201:enzymology; D016328:NF-kappa B / Q000378:metabolism; D000071876:Prostaglandin-E Synthases; D015854:Up-Regulation
chemical_list: D018955:CD36 Antigens; D016209:Interleukin-8; D008077:Lipoproteins, LDL; D016328:NF-kappa B; C099252:oxidized low density lipoprotein; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone