title: Growth kinetics of amyloid-like fibrils derived from individual subunits of soy β-conglycinin.
abstract: The amyloid-like fibrillation of soy β-conglycinin subunits (α, α', and β) upon heating (0-20 h) at 85 °C and pH 2.0 was characterized using dynamic light scattering, circular dichroism (CD), binding to amyloid dyes (Thioflavin T and Congo red), and atomic force microscopy. The fibrillation of all three subunits was accompanied by progressive polypeptide hydrolysis. The hydrolysis behaviors, fibrillation kinetics, and morphologies of amyloid-like fibrils considerably varied among α, α', and β subunits. Faster hydrolysis rates and special fragments were observed for the α and α' subunits compared to the β subunit. However, the order of the fibrillation rate and capacity to form β-sheets was α' > β > α, as evidenced by CD and Thioflavin T data. Moreover, sequential growth of twisted screw-structure fibrils, leading to macroscopic fibrils with distinct morphological characteristics, was observed for β-conglycinin and individual subunits. The different fibrillation kinetics and morphologies of α, α', and β subunits appear to be associated with the differences in the amino acid composition and typical sequence of peptides. Besides, the disruption of ordered structure of fibrils occurred upon further heating (6-20 h) due to extensive hydrolysis. These results would suggest that all subunits are involved in the fibrillation of β-conglycinin, following multiple steps including polypeptide hydrolysis, assembly to amyloid structure, and growth into macroscopic fibrils with a fibril shaving process.
authors.affiliation: Department of Food Science and Technology, South China University of Technology, Guangzhou 510640, People's Republic of China.
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Wang
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Yang
authors.forename: Shou-Wei
authors.initials: SW
authors.lastname: Yin
authors.forename: De-Bao
authors.initials: DB
authors.lastname: Yuan
authors.forename: Ning
authors.initials: N
authors.lastname: Xia
authors.forename: Jun-Ru
authors.initials: JR
authors.lastname: Qi
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry*; D002942:Circular Dichroism; D005916:Globulins / Q000737:chemistry*; D006358:Hot Temperature; D006868:Hydrolysis; D008027:Light; D018625:Microscopy, Atomic Force; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry; D012542:Scattering, Radiation; D055314:Seed Storage Proteins / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D000682:Amyloid; D052179:Antigens, Plant; D005916:Globulins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max