title: [Refining sample preparation procedures for solution 3P-NMR analysis of organic P in sediment].
abstract: Solution phosphorus-31 nuclear magnetic resonance spectroscopy (31P-NMR) is a novel technology in characterization of organic phosphorus (P) composition in sediments. This study was aim to refine sample preparation procedures for 31P-NMR, including determination of sample pretreatment, extraction time, sample to extractant ratio and concentration method. The results showed that recovery of P with air dried sample treatment was higher than that for the fresh samples. Most of the P was extracted within the first 8 hours, and the time could extend to 12 to 16 hours in order to improve the recovery of P. Using the extraction ratio [m(sediment): V(extractant)] of 1:8 received more spectral signals compared to 1:20 when both the extracts were concentrated at the same time. No evident loss of P was found when the extract was concentrated at 28 degrees C followed by centrifugation under low temperature.
authors.affiliation: Institute of Natural Resources and Environment, Henan University, Kaifeng 475004, China. bxling@163.com
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Bai
authors.forename: Yun-Kai
authors.initials: YK
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Ding
mesh_terms: D019015:Geologic Sediments / Q000032:analysis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010755:Organophosphates / Q000032:analysis*; D010759:Phosphorus Isotopes / Q000737:chemistry*
chemical_list: D010755:Organophosphates; D010759:Phosphorus Isotopes