title: Vaccine-induced cross-genotype reactive neutralizing antibodies against hepatitis C virus.
abstract: We detected cross-reactive neutralizing antibodies (NtAb) against hepatitis C virus (HCV) in chimpanzees vaccinated with HCV-1 (genotype 1a) recombinant E1/E2 envelope glycoproteins. Five vaccinated chimpanzees, protected following HCV-1 challenge, were initially studied using the heterologous H77 (genotype 1a) HCVpp assay. All animals had developed NtAb after the second vaccination; 4 animals had reciprocal titers of ≥200 at the time of challenge. Using genotypes 1a-6a HCV pseudoparticles (HCVpp) and cell culture-derived HCV (HCVcc) assays, cross-reactive NtAb were detected against 1a, 4a, 5a, and 6a, with limited reactivity against 2a and 3a. Our study provides encouragement for the development of a recombinant envelope-based vaccine against hepatitis C.
authors.affiliation: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Meunier
authors.forename: Judith M
authors.initials: JM
authors.lastname: Gottwein
authors.forename: Michael
authors.initials: M
authors.lastname: Houghton
authors.forename: Rodney S
authors.initials: RS
authors.lastname: Russell
authors.forename: Suzanne U
authors.initials: SU
authors.lastname: Emerson
authors.forename: Jens
authors.initials: J
authors.lastname: Bukh
authors.forename: Robert H
authors.initials: RH
authors.lastname: Purcell
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis* / Q000097:blood; D003429:Cross Reactions; D005434:Flow Cytometry; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D019698:Hepatitis C, Chronic / Q000276:immunology* / Q000517:prevention & control; D002679:Pan troglodytes; D012189:Retrospective Studies; D014611:Vaccination / Q000379:methods; D014614:Vaccines, Synthetic / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014761:Viral Hepatitis Vaccines / Q000276:immunology* / Q000592:standards
chemical_list: D057134:Antibodies, Neutralizing; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014761:Viral Hepatitis Vaccines