title: Neuropeptidases.
abstract: The control of neuropeptide function is partially accomplished by aminopeptidases (neuropeptidases), which are the most abundant proteolytic enzymes in brain. Their analysis represents an important and quick tool to reflect the functional status of their endogenous substrates. Here, we describe an improved fluorometric method for the determination of neuropeptidase activities based on the fluorescence produced by β-naphthylamine when released from the artificial substrates aminoacyl-β-naphthylamides (arylamides) under the hydrolytic action of these enzymes.
authors.affiliation: Unit of Physiology, Department of Health Sciences, University of Jaén, Jaén, Spain. msanchez@ujaen.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Ramírez
authors.forename: Isabel
authors.initials: I
authors.lastname: Prieto
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Banegas
authors.forename: Ana B
authors.initials: AB
authors.lastname: Segarra
authors.forename: Francisco
authors.initials: F
authors.lastname: Alba
mesh_terms: D015081:2-Naphthylamine / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism
chemical_list: D009479:Neuropeptides; D015081:2-Naphthylamine; D000626:Aminopeptidases