title: The effect of HMGB1 A box on lung injury in mice with acute pancreatitis.
abstract: The objective of this study is to observe the effect of high-mobility group protein B1 A Box (HMGB1 A) box on lung injury in mice with acute pancreatitis and its effect on the level of high-mobility group protein B1 (HMGB1) in lung, to explore the mechanism. A total of 60 male Institute of Cancer Research mice were randomly divided into control group (n = 30) and treatment group (n = 30). Severe acute pancreatitis mice model was induced by 20% L-Arg intraperitoneal injection. The recombination HMGB1 A box was used in treatment after modeling. All the mice were killed under anesthesia at 24 and 48 h after the modeling injection. The level of HMGB1 and activity of myeloperoxidase (MPO) in lung were measured. The pathological changes of lung were observed. The level of HMGB1 in lung of A box treatment group decreased more significantly 24 h and 48 h after modeling compared with control group. The activity of MPO in lung of A box treatment group decreased more significantly 24 h after modeling compared with control group. The lung tissue pathologic score of A box treatment group decreased more significantly 48 h after modeling compared with control group. HMGB1 expression levels in the lungs were positively related to histological score of injured lung in acute pancreatitis. It indicates that HMGB1 A box is remarkably protective to lung injury induced by acute pancreatitis.
authors.affiliation: Department of General Surgery, Provincial Hospital affiliated to Shandong University, Jinan, China.
authors.forename: Xiangqian
authors.initials: X
authors.lastname: Kong
authors.forename: Caiqing
authors.initials: C
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Jin
authors.forename: Xuejun
authors.initials: X
authors.lastname: Wu
authors.forename: Shiyi
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhenyue
authors.initials: Z
authors.lastname: Zhong
authors.forename: Qian
authors.initials: Q
authors.lastname: Feng
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Hai
authors.initials: H
authors.lastname: Yuan
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D055370:Lung Injury / Q000188:drug therapy* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010195:Pancreatitis / Q000235:genetics / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation
chemical_list: D000199:Actins; D024243:HMGB1 Protein; D009195:Peroxidase