title: Effects of fruit and vegetable low molecular mass fractions on gene expression in gingival cells challenged with Prevotella intermedia and Actinomyces naeslundii.
abstract: Low molecular mass (LMM) fractions obtained from extracts of raspberry, red chicory, and Shiitake mushrooms have been shown to be an useful source of specific antibacterial, antiadhesion/coaggregation, and antibiofilm agent(s) that might be used for protection towards caries and gingivitis. In this paper, the effects of such LMM fractions on human gingival KB cells exposed to the periodontal pathogens Prevotella intermedia and Actinomyces naeslundii were evaluated. Expression of cytokeratin 18 (CK18) and β4 integrin (β4INT) genes, that are involved in cell proliferation/differentiation and adhesion, and of the antimicrobial peptide β2 defensin (HβD2) in KB cells was increased upon exposure to either live or heat-killed bacteria. All LMM fractions tested prevented or reduced the induction of gene expression by P. intermedia and A. naeslundii depending on the experimental conditions. Overall, the results suggested that LMM fractions could modulate the effects of bacteria associated with periodontal disease in gingival cells.
authors.affiliation: DIPTERIS, University of Genova, Corso Europa 26, 16132 Genova, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Canesi
authors.forename: Cristina
authors.initials: C
authors.lastname: Borghi
authors.forename: Monica
authors.initials: M
authors.lastname: Stauder
authors.forename: Peter
authors.initials: P
authors.lastname: Lingström
authors.forename: Adele
authors.initials: A
authors.lastname: Papetti
authors.forename: Jonathan
authors.initials: J
authors.lastname: Pratten
authors.forename: Caterina
authors.initials: C
authors.lastname: Signoretto
authors.forename: David A
authors.initials: DA
authors.lastname: Spratt
authors.forename: Mike
authors.initials: M
authors.lastname: Wilson
authors.forename: Egija
authors.initials: E
authors.lastname: Zaura
authors.forename: Carla
authors.initials: C
authors.lastname: Pruzzo
mesh_terms: D000190:Actinomyces / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D018651:Cichorium intybus / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects*; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000382:microbiology*; D006801:Humans; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018713:Prevotella intermedia / Q000187:drug effects; D020942:Shiitake Mushrooms / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts