title: Rapid identification of triterpenoid sulfates and hydroxy fatty acids including two new constituents from Tydemania expeditionis by liquid chromatography-mass spectrometry.
abstract: Tydemania expeditionis Weber-van Bosse (Udoteaceae) is a weakly calcified green alga. In the present paper, liquid chromatography coupled with photodiode array detection and electrospray mass spectrometry was developed to identify the fingerprint components. A total of four triterpenoid sulfates and three hydroxy fatty acids in the ethyl acetate fraction of the crude extract were structurally characterized on the basis of retention time, online UV spectrum, and mass fragmentation pattern. Furthermore, a detailed liquid chromatography-mass spectrometry analysis revealed two new hydroxy fatty acids, which were then prepared and characterized by extensive nuclear magnetic resonance (NMR) analyses. The proposed method provides a scientific and technical platform for the rapid identification of triterpenoid sulfates and hydroxy fatty acids in similar marine algae and terrestrial plants.
authors.affiliation: Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou, China.
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Zhang
authors.forename: Julia
authors.initials: J
authors.lastname: Kubanek
authors.forename: Mark E
authors.initials: ME
authors.lastname: Hay
authors.forename: William
authors.initials: W
authors.lastname: Aalbersberg
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.forename: Ren-Wang
authors.initials: RW
authors.lastname: Jiang
mesh_terms: D000085:Acetates / Q000737:chemistry; D000460:Chlorophyta / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry* / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013431:Sulfates / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000085:Acetates; D005227:Fatty Acids; D013431:Sulfates; D014315:Triterpenes; C007650:ethyl acetate