title: [Study of generational risk in deafness inflicted couples using deafness gene microarray technique].
abstract: OBJECTIVE
  To explored the significance of screening the gene mutations of deafness related in deaf-mute (deaf & dumb) family using DNA microarray.
  
  
  METHODS
  Total of 52 couples of deaf-mute were recruited from Changchun deaf-mute community. With an average age of (58.3 ± 6.7) years old (x(-) ± s). Blood samples were obtained with informed consent. Their genomic DNA was extracted from peripheral blood and PCR was performed. Nine of hot spot mutations in four most common deafness pathologic gene were examined with the DNA microarray, including GJB2, GJB3, PDS and mtDNA 12S rRNA genes. At the same time, the results were verified with the traditional methods of sequencing. Fifty of normal people served as a control group.
  
  
  RESULTS
  All patients were diagnosed non-syndromic sensorineural hearing loss by subjective pure tone audiometry. Thirty-two of 104 cases appeared GJB2 gene mutation (30.7%), the mutation sites included 35delG, 176del16, 235delC and 299delAT. Eighteen of 32 cases of GJB2 mutations were 235delC (59.1%). Seven of 104 cases appeared SLC26A4 gene IVS7-2 A > G mutation. Questionnaire survey and gene diagnosis revealed that four of 52 families have deaf offspring (7.6%). When a couple carries the same gene mutation, the risk of their children deafness was 100%. The results were confirmed with the traditional methods of sequencing.
  
  
  CONCLUSIONS
  There is a high risk of deafness if a deaf-mute family is planning to have a new baby. It is very important and helpful to avoid deaf newborns again in deaf-mute family by DNA microarray.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Shu-yuan
authors.initials: SY
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: DU
mesh_terms: D016022:Case-Control Studies; D000072259:Connexin 26; D017630:Connexins; D004252:DNA Mutational Analysis; D003638:Deafness / Q000175:diagnosis / Q000235:genetics* / Q000517:prevention & control*; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D017630:Connexins; C000607042:GJB2 protein, human; D000072259:Connexin 26