title: [Nerve growth factor increases sodium current via interferon regulatory factor-1 pathway in rat pheochromocytoma cells].
abstract: OBJECTIVE
  To explore the effect of nerve growth factor(NGF) and interferon regulatory factor-1(IRF-1) on sodium current change of sensory neuron in rat pheochromocytoma cells.
  
  
  METHODS
  Sensory neuron rat pheochromocytoma cells were stimulated by different concentrations of NGF(0-200 ng/mL), the IRF-1 mRNA levels were examined by real-time PCR, and the activation of IRF-1 was examined by Western blot. The sodium current change was recorded by patch clamp.
  
  
  RESULTS
  Low concentration of NGF improved the sodium current, which was concentration dependent. When exposed to high concentration of NGF, the expression of IRF-1 mRNA in PC-12 was improved. Low concentration of NGF resulted in IRF-1 intronuclear transporting, and the expression was not affected. Sodium current did not occur in PC-12 cells when IRF-1 was blocked.
  
  
  CONCLUSIONS
  NGF can improve the sodium current in PC-12 cells concentration-dependently, and the improvement is regulated by IRF-1.
authors.affiliation: Department of Respiratory Medicine, Xiangya Hospital, Central Sounth Univerdity, Changsha 410008, China.
authors.forename: Yemu
authors.initials: Y
authors.lastname: Zhu
authors.forename: Pinhua
authors.initials: P
authors.lastname: Pan
authors.forename: Hongyi
authors.initials: H
authors.lastname: Tan
authors.forename: Chengping
authors.initials: C
authors.lastname: Hu
mesh_terms: D000818:Animals; D050856:Interferon Regulatory Factor-1 / Q000235:genetics / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000494:pharmacology*; D016716:PC12 Cells; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D015222:Sodium Channels / Q000187:drug effects*
chemical_list: D050856:Interferon Regulatory Factor-1; D012333:RNA, Messenger; D015222:Sodium Channels; D020932:Nerve Growth Factor