title: [Effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell].
abstract: The present paper is aimed to investigate the effects of pulse electrical stimulation on mutual adhesion of vascular endothelial cell and endothelial progenitor cell (EPC). EPC was induced from periphery blood, labeled with fluorescence dye and then co-cultured with vascular endothelial cell. With a fixed electric voltage and frequency of 5V and 5Hz, respectively, the co-culture system was continually stimulated for 24h under different pulse width, 1, 3, 6 and 9ms. After pulse stimulation, fluorescence intensity of adherent labeled EPC was measured and converted to fluorescence ratio. Compared to that in the control group, fluorescence ratio of 3 ms and 6 ms group were significantly larger, while that in the 9 ms group was lower. The peak fluorescence ratio value was appeared at 6 ms group. It is indicated that suitable pulse electrical stimulation could benefit the adhesion of endothelial cell and EPC. All these results provide a new theoretical basis about why electrical stimulation could contribute to neovascularization.
authors.affiliation: Department of Clinical Laboratory Center, Nan fang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Weiqi
authors.initials: W
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Shuyan
authors.initials: S
authors.lastname: Guo
mesh_terms: D000328:Adult; D002448:Cell Adhesion / Q000528:radiation effects*; D002478:Cells, Cultured; D018920:Coculture Techniques; D004558:Electric Stimulation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000528:radiation effects; D006801:Humans; D008297:Male; D055815:Young Adult