title: Process for biodiesel production from Cryptococcus curvatus.
abstract: The objective of the current report is process optimization for economical production of lipids by the well known oleaginous yeast Cryptococcus curvatus and conversion of the lipids to biodiesel. A high cell density fed-batch cultivation on low cost substrate viz. crude glycerol resulted in a dry biomass and oil yield of up to 69 g/L and 48% (w/w), respectively. The process was scaled up easily to 26 L. The oil extraction process was also optimized using environmentally safe solvents. The oil profile indicated a high oleic acid content followed by palmitic acid, stearic acid and linoleic acid. The oil was trans-esterified to biodiesel and thoroughly characterized. This is the first end to end report on production of biodiesel from the C. curvatus oil.
authors.affiliation: Industrial Biotechnology Group, Reliance Life Sciences Pvt. Ltd., India.
authors.forename: Meikandhan
authors.initials: M
authors.lastname: Thiru
authors.forename: Santosh
authors.initials: S
authors.lastname: Sankh
authors.forename: Vidhya
authors.initials: V
authors.lastname: Rangaswamy
mesh_terms: D001419:Bacteria / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D056804:Biofuels / Q000032:analysis* / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D003454:Cryptococcus / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D031286:Jatropha / Q000737:chemistry; D010938:Plant Oils / Q000302:isolation & purification
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D010938:Plant Oils