title: Evaluation of enzymes inhibition activities of medicinal plant from Burkina Faso.
abstract: The aim of the present study was to evaluate some enzymes inhibitory effects of 11 plant species belonging to 9 families from Burkina Faso. Methanolic extracts were used for their Glutathione-s-transferase (GST), Acetylcholinesterase (AChE), Carboxylesterase (CES) and Xanthine Oxidase (XO) inhibitory activities at final concentration of 100 microg mL(-1). The total phenolics, flavonoids and tannins were also determined spectrophotometrically using Folin-Ciocalteu, AlCl3 and ammonium citrate iron reagents, respectively. Among the 11 species tested, the best inhibitory percentages were found with Euphorbia hirta, Sclerocarya birrea and Scoparia dulcis (inhibition > 40%) followed by Annona senegalensis, Annona squamosa, Polygala arenaria and Ceratotheca sesamoides (inhibition > 25%). The best total phenolic and tannin contents were found with S. birrea with 56.10 mg GAE/100 mg extract and 47.75 mg TAE/100 mg extract, respectively. E hirta presented the higher total flavonoids (9.96 mg QE/100 mg extract). It's was found that Sclerocarya birrea has inhibited all enzymes at more than 30% and this activity is correlated to total tannins contents. Contrary to S. birrea, the enzymatic activities of E. hirta and S. dulcis are correlated to total flavonoids contents. Present findings suggest that the methanolic extracts of those plant species are potential inhibitors of GST, AChE, CES and XO and confirm their traditional uses in the treatment of mental disorders, gout, painful inflammations and cardiovascular diseases.
authors.affiliation: Laboratoire de Biochimie et de Chimie Appliquées, Université de Ouagadougou, 09 BP 848 Ouagadougou 09, Burkina Faso.
authors.forename: Mindiédiba Jean
authors.initials: MJ
authors.lastname: Bangou
authors.forename: Martin
authors.initials: M
authors.lastname: Kiendrebeogo
authors.forename: Nâg-Tiero Roland
authors.initials: NT
authors.lastname: Meda
authors.forename: Ahmed Yacouba
authors.initials: AY
authors.lastname: Coulibaly
authors.forename: Moussa
authors.initials: M
authors.lastname: Compaoré
authors.forename: Boukaré
authors.initials: B
authors.lastname: Zeba
authors.forename: Jeanne
authors.initials: J
authors.lastname: Millogo-Rasolodimby
authors.forename: Odile Germaine
authors.initials: OG
authors.lastname: Nacoulma
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002050:Burkina Faso; D043182:Carboxylesterase / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry; D005982:Glutathione Transferase / Q000378:metabolism; D000432:Methanol / Q000737:chemistry; D019800:Phenol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000378:metabolism*; D059808:Polyphenols; D013053:Spectrophotometry / Q000379:methods; D013552:Swine; D013634:Tannins / Q000737:chemistry; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013634:Tannins; D019800:Phenol; D014969:Xanthine Oxidase; D005982:Glutathione Transferase; D043182:Carboxylesterase; D000110:Acetylcholinesterase; D000432:Methanol