title: Rapid real-time electrical detection of proteins using single conducting polymer nanowire-based microfluidic aptasensor.
abstract: Single polypyrrole (PPy) nanowire-based microfluidic aptasensors were fabricated using a one-step electrochemical deposition method. The successful incorporation of the aptamers into the PPy nanowire was confirmed by fluorescence microscopy image. The microfluidic aptasensor showed responses to IgE protein solutions in the range from 0.01 nM to 100 nM, and demonstrated excellent specificity and sensitivity with faster response and rapid stabilization times (~20 s). At the lowest examined IgE concentration of 0.01 nM, the microfluidic aptasensor still exhibited ~0.32% change in the conductance. The functionality of this aptasensor was able to be regenerated using an acid treatment with no major change in sensitivity. In addition, the detection of cancer biomarker MUC1 was performed using another microfluidic aptasensor, which showed a very low detection limit of 2.66 nM MUC1 compared to commercially available MUC1 diagnosis assay (800 nM).
authors.affiliation: Department of Electrical and Computer Engineering, Swanson School of Engineering, University of Pittsburgh, Pittsburgh, PA 15261, USA.
authors.forename: Jiyong
authors.initials: J
authors.lastname: Huang
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
authors.forename: Innam
authors.initials: I
authors.lastname: Lee
authors.forename: Yushi
authors.initials: Y
authors.lastname: Hu
authors.forename: Xinyan Tracy
authors.initials: XT
authors.lastname: Cui
authors.forename: Minhee
authors.initials: M
authors.lastname: Yun
mesh_terms: D052158:Aptamers, Peptide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003199:Computer Systems; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D043942:Nanotubes / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D052158:Aptamers, Peptide; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole