title: Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells.
abstract: The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the NDs' intrinsic fluorescence from embedded color-center defects. Cell toxicity of these coated NDs is shown to be low. Consistent with the internalization efficacy, a specific inhibition of EWS/Fli-1 gene expression is shown at the mRNA and protein level by the ND-vectorized siRNA in a serum-containing medium.
authors.affiliation: Université Paris-Sud, Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, F-91405 Orsay, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Alhaddad
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Adam
authors.forename: Jacques
authors.initials: J
authors.lastname: Botsoa
authors.forename: Géraldine
authors.initials: G
authors.lastname: Dantelle
authors.forename: Sandrine
authors.initials: S
authors.lastname: Perruchas
authors.forename: Thierry
authors.initials: T
authors.lastname: Gacoin
authors.forename: Christelle
authors.initials: C
authors.lastname: Mansuy
authors.forename: Solange
authors.initials: S
authors.lastname: Lavielle
authors.forename: Claude
authors.initials: C
authors.lastname: Malvy
authors.forename: François
authors.initials: F
authors.lastname: Treussart
authors.forename: Jean-Rémi
authors.initials: JR
authors.lastname: Bertrand
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics / Q000628:therapy*; D045744:Cell Line, Tumor; D051379:Mice; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D058612:Nanodiamonds; D034741:RNA, Small Interfering / Q000235:genetics*; D012512:Sarcoma, Ewing / Q000235:genetics / Q000628:therapy*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D058612:Nanodiamonds; D034741:RNA, Small Interfering