title: [Study on activities and protein and gene expression of renal H(+)-K(+)-ATPase in rats subchronic exposed to trimethyltin chloride].
abstract: OBJECTIVE
  To study the activity, protein and gene expression of renal HK-ATPase (HKA) in rats subchronic exposed to trimethyltin chloride (TMT).
  
  
  METHODS
  In subchronic toxic test (14-week), 55 female SD rats (age, 6 weeks) were divided randomly into 5 groups: control, low, medium, high and super high dosage, respectively, which drank water with TMT of 0, 8.20, 32.81, 131.25 and 262.50 microg x kg(-1) x d(-1) for 14 weeks. Then serum K+ levels were measured; the activities of HK-ATPase (HKA) in kidneys were detected by the method of determinated phosphorus content; Western Blot assay and real-time PCR were used to exam the protein and mRNA expression levels of HKA in kidneys, respectively.
  
  
  RESULTS
  The serum K+ level in super-high dosage group was (5.6 +/- 0.4) mmol/L, which was significantly lower than that [(6.9 +/- 0.3) mmol/L] in control group (P < 0.01). The HKA enzymatic activity of kidneys in low and super high dosage groups was 4.50 +/- 1.45 and 4.55 +/- 0.72 micromolPi x mg prot(-1)h(-1), respectively, which were significantly lower than that (6.55 +/- 0.77 micromol Pi x mg prot(-1) h(-1)) in control group (P < 0.05).
  
  
  CONCLUSIONS
  When rats were exposed subchronic to TMT, the renal HKA activity could reduce, but the expression levels of HKA protein and mRNA did not decrease.
authors.affiliation: Guangdong Prevention & Treatment Center for Occupational Diseases, Guangzhou 510300, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.forename: Gang
authors.initials: G
authors.lastname: Sui
authors.forename: Nan-Chun
authors.initials: NC
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Zi
authors.initials: Z
authors.lastname: Yang
authors.forename: Guan-Chao
authors.initials: GC
authors.lastname: Lai
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhong-Ning
authors.initials: ZN
authors.lastname: Lin
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Tang
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D059550:Toxicity Tests, Subchronic; D014298:Trimethyltin Compounds / Q000633:toxicity*
chemical_list: D014298:Trimethyltin Compounds; C040533:trimethyltin chloride; D017506:H(+)-K(+)-Exchanging ATPase