title: Induction of decreased fecundity by tetanus toxoid hyper-immunization in C57BL/6 mice depends on the applied adjuvant.
abstract: It has already been shown that tetanus toxoid (TTd) hyper-immunization is a suitable experimental method for creating the animal model of antiphospholipid syndrome (APS) in BALB/c mice. The severity of APS pathology in BALB/c mice mainly correlates to the affinity of anti-β(2) glycoprotein I (β(2)GPI) antibodies. In this study we have investigated reproductive pathology induced in C57BL/6 mice by TTd hyper-immunization using a combination of different pretreatments (complete Freund's adjuvant or glycerol) and adjuvants (alhydrogel or glycerol). A decrease in fecundity was recorded in only C57BL/6 mice immunized with alhydrogel adjuvant, irrespective of the kind of applied pretreatment; it was associated with an increase in abundance of low affinity anti-β(2)GPI IgG antibodies and Th1 prevalence.
authors.affiliation: Institute of Virology, Vaccines and Sera - Torlak, Belgrade, Serbia. z92irena@yahoo.com
authors.forename: Irena
authors.initials: I
authors.lastname: Zivkovic
authors.forename: Vladimir
authors.initials: V
authors.lastname: Petrusic
authors.forename: Marijana
authors.initials: M
authors.lastname: Stojanovic
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Inic-Kanada
authors.forename: Ivana
authors.initials: I
authors.lastname: Stojicevic
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Dimitrijevic
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000915:Antibody Affinity; D000941:Antigens / Q000032:analysis; D016736:Antiphospholipid Syndrome / Q000276:immunology*; D002308:Cardiolipins / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005298:Fertility / Q000276:immunology*; D005620:Freund's Adjuvant / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D013745:Tetanus Toxoid / Q000276:immunology*; D018417:Th1 Cells / Q000502:physiology; D018418:Th2 Cells / Q000502:physiology; D053482:beta 2-Glycoprotein I / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; D002308:Cardiolipins; D007074:Immunoglobulin G; D013745:Tetanus Toxoid; D053482:beta 2-Glycoprotein I; D000536:Aluminum Hydroxide; D005620:Freund's Adjuvant; D005990:Glycerol