title: An odorant-binding protein as a new allergen from Siberian hamster (Phodopus sungorus).
abstract: A case of anaphylaxis following a bite from a Siberian hamster (SH; Phodopus sungorus) is described. Skin prick tests with hair, urine and salivary gland extracts from SH were positive, while the tests were negative for hair extracts from other rodents. IgE immunoblotting with the patient serum revealed 3 IgE-binding bands of about 18, 21 and 23 kDa. When the patient's serum was preincubated with rabbit, mouse and gerbil hair extracts, no inhibition of the 3 SH IgE-binding bands was demonstrated. Proteins extracted from the 3 bands were analyzed by N-terminal sequencing and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry, and peptides were sequenced. IgE-binding bands were identified as being an odorant-binding protein belonging to the lipocalin family. Analysis of the 3 IgE-binding bands found in the hair, urine and salivary glands of SH showed a new allergenic protein lacking cross-reactivity with allergens from other rodents. The 3 bands likely correspond to isoforms of a single allergen.
authors.affiliation: Department of Allergy, Fundación Jiménez Díaz, Av. Reyes Católicos 2, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Torres
authors.forename: C
authors.initials: C
authors.lastname: Pastor-Vargas
authors.forename: M
authors.initials: M
authors.lastname: de las Heras
authors.forename: F
authors.initials: F
authors.lastname: Vivanco
authors.forename: Javier
authors.initials: J
authors.lastname: Cuesta
authors.forename: J
authors.initials: J
authors.lastname: Sastre
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000175:diagnosis / Q000276:immunology / Q000628:therapy; D000799:Angioedema / Q000175:diagnosis / Q000276:immunology; D000818:Animals; D006224:Cricetinae; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D008969:Molecular Sequence Data; D017111:Phodopus / Q000276:immunology*; D018035:Receptors, Odorant / Q000276:immunology*; D012882:Skin Tests; D055815:Young Adult
chemical_list: D000485:Allergens; D018035:Receptors, Odorant; C045648:odorant-binding protein; D007073:Immunoglobulin E