title: Characterization of circulating HIV type 1 env genes in plasma of two antiretroviral-naive slow progressing patients with broad neutralizing antibody response with evidence of recombination.
abstract: In the present study, we investigated genetic divergence between complete autologous HIV-1 env genes amplified directly from plasma of two antiretroviral-naive, slow progressing Indian patients with broad neutralizing antibody response. All the envelope (Env) clones obtained from one patient (LT1) belonged to subtype C; the second patient (LT5) harbored quasispecies comprised of pure B, C, and B/C recombinants with distinct breakpoints indicative of dual infection with genetically distinct strains. Further characterization of these Envs would provide insight into the biological properties under strong humoral immune response.
authors.affiliation: Department of Molecular Virology, National AIDS Research Institute, Bhosari, Pune, India.
authors.forename: Sampurna
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ringe
authors.forename: Ajit
authors.initials: A
authors.lastname: Patil
authors.forename: Ramesh
authors.initials: R
authors.lastname: Paranjape
authors.forename: Jayanta
authors.initials: J
authors.lastname: Bhattacharya
mesh_terms: D000328:Adult; D057134:Antibodies, Neutralizing / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D018740:Genetic Heterogeneity; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D006679:HIV Seropositivity / Q000235:genetics* / Q000276:immunology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D057134:Antibodies, Neutralizing; D015699:HIV Envelope Protein gp120