title: Evaluation of four commercial real-time PCR assays for detection of Bordetella spp. in nasopharyngeal aspirates.
abstract: We evaluated the performances of 4 commercial real-time PCR kits for Bordetella pertussis IS481 sequence detection in nasopharyngeal aspirates by comparison with an in-house real-time PCR assay. Among them, the Simplexa Bordetella pertussis/parapertussis assay (Focus Diagnostics), the SmartCycler Bordetella pertussis/parapertussis assay (Cepheid), and Bordetella R-gene (Argene) present sensitivities over 90%. One kit proved unsuitable for routine clinical use.
authors.affiliation: CHRU de Tours, Service de Bactériologie-Virologie, Hôpital Bretonneau, Tours, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lanotte
authors.forename: Chloé
authors.initials: C
authors.lastname: Plouzeau
authors.forename: Christophe
authors.initials: C
authors.lastname: Burucoa
authors.forename: Carole
authors.initials: C
authors.lastname: Grélaud
authors.forename: Sophie
authors.initials: S
authors.lastname: Guillot
authors.forename: Nicole
authors.initials: N
authors.lastname: Guiso
authors.forename: Fabien
authors.initials: F
authors.lastname: Garnier
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D011933:Reagent Kits, Diagnostic