title: [Assessment of the application of variable-number tandem repeat loci of Salmonella Enteritidis in subtyping multiple-locus variable-number tandem repeat analysis].
abstract: OBJECTIVE
  To evaluate the feasibility of the application of variable-number tandem repeat (VNTR) loci of Salmonella Enteritidis (S. enteritidis) in subtyping mutiple-locus variable-number tandem repeat analysis (MLVA).
  
  
  METHODS
  A total of 16 isolates of S.enteritidis from different place and time in China were preliminarily assessed by choosing 11 reported VNTR loci, the loci with single amplified bands were picked to subtype all 104 S. enteritidis isolates. The isolates were also analyzed by pulse field gel electrophoresis (PFGE) to compare the superiority or inferiority of MLVA method and PFGE method.
  
  
  RESULTS
  Seven VNTR loci were selected from the preliminary screening to expand the analysis, and the 7 VNTR loci had grouped 104 of S.enteritidis isolates into either 16 MLVA subtypes or 22 PFGE subtypes, with the D value at 0.7222 and 0.7974, respectively. Comparing with the isolates in MLVA subtypes, the isolates in PFGE showed a stronger resolving power. Meanwhile the results in PFGE showed a more disperse frequency distribution than those in MLVA.
  
  
  CONCLUSIONS
  These results indicate that some VNTR locus which have shown a good polymorphism internationally, may fail to show polymorphism in China, thereby, more VNTR loci should be included in MLVA and the wide screening may benefit the unity of global laboratorial methods.
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Han
authors.forename: Hai-jian
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Cui
authors.forename: Mei-ying
authors.initials: MY
authors.lastname: Yan
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics*