title: Exploring isonitrile-based click chemistry for ligation with biomolecules.
abstract: We show here that isonitriles can perform click reactions with tetrazines in aqueous media, making them promising candidates for ligation reactions in chemical biology and polymer chemistry. This is the first time that a [4+1] cycloaddition has been used as a biocompatible ligation reaction.
authors.affiliation: University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge, UK CB2 1EW.
authors.forename: Henning
authors.initials: H
authors.lastname: Stöckmann
authors.forename: André A
authors.initials: AA
authors.lastname: Neves
authors.forename: Shaun
authors.initials: S
authors.lastname: Stairs
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
authors.forename: Finian J
authors.initials: FJ
authors.lastname: Leeper
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D057930:Click Chemistry; D003500:Cyclization; D006868:Hydrolysis; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D013777:Tetrazoles / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D009570:Nitriles; D013777:Tetrazoles