title: The R2TP complex: discovery and functions.
abstract: The two closely related AAA+family ATPases Rvb1 and Rvb2 are part of several critical multiprotein complexes, and, thus, are involved in a wide range of cellular processes including chromatin remodelling, telomerase assembly, and snoRNP biogenesis. It was found that Rvb1 and Rvb2 form a tight functional complex with Pih1 (Protein interacting with Hsp90) and Tah1 (TPR-containing protein associated with Hsp90), which are two Hsp90 interactors. We named the complex R2TP. The complex was originally isolated from Saccharomyces cerevisiae and was, subsequently, identified in mammalian cells. R2TP was found to be required for box C/D snoRNP biogenesis in yeast and mammalian cells. More recently, several studies revealed that the complex is also involved in multiple biological processes including apoptosis, phosphatidylinositol-3 kinase-related protein kinase (PIKK) signalling, and RNA polymerase II assembly. In this review, we describe the discovery of the complex and discuss the emerging critical roles that R2TP plays in distinct cellular processes.
authors.affiliation: Department of Biochemistry, University of Totonto, Toronto, Ontario, Canada M5S 1A8.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Kakihara
authors.forename: Walid A
authors.initials: WA
authors.lastname: Houry
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism*; D004398:Dyneins / Q000378:metabolism; D006801:Humans; D018832:Molecular Chaperones / Q000737:chemistry / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D012319:RNA Polymerase II / Q000378:metabolism; D020718:Ribonucleoproteins, Small Nucleolar / Q000096:biosynthesis; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; C497702:PIH1 protein, S cerevisiae; D020718:Ribonucleoproteins, Small Nucleolar; D029701:Saccharomyces cerevisiae Proteins; C571197:Tah1 protein, S cerevisiae; D014157:Transcription Factors; D019869:Phosphatidylinositol 3-Kinases; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases; C428198:RVB1 protein, S cerevisiae; C428199:RVB2 protein, S cerevisiae; D004265:DNA Helicases; D004398:Dyneins