title: Investigation into the anti-inflammatory and antigranuloma activity of Colchicum luteum Baker in experimental models.
abstract: The present study evaluates the anti-inflammatory and antigranuloma activity of CLHE in experimental models, viz. carrageenan-induced paw edema, subcutaneous cotton pellet implantation-induced granuloma formation, and complete Freund's adjuvant-induced stimulation of peritoneal macrophages in rats. Serum TNF-alpha, IL-6, and IL-1 beta levels were estimated as markers for global effects of inflammation. TNF-R1 protein expression was estimated in stimulated peritoneal macrophages. There was a significant reduction (P < 0.05) of paw edema in the CLHE-treated groups as compared to control. In the cotton pellet-induced granuloma model, there was a significant (P < 0.05) reduction in the dry granuloma weight and serum TNF-alpha, IL-6, and IL-1beta levels in the CLHE-treated group as compared to control. Immunoblot analysis for TNF-R1 also demonstrated a significant reduction in the receptor protein expression on stimulated macrophages. Result of the present study thus demonstrates and validates the antigranuloma activity of CLHE.
authors.affiliation: Department of Pharmacology, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi 110029, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Nair
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.forename: Surender
authors.initials: S
authors.lastname: Singh
authors.forename: Y K
authors.initials: YK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy; D002351:Carrageenan; D003079:Colchicum; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005620:Freund's Adjuvant / Q000276:immunology; D006099:Granuloma / Q000188:drug therapy*; D007249:Inflammation / Q000188:drug therapy*; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan; D005620:Freund's Adjuvant