title: [Effects of N-cadherin expression on cell cycle, cell apoptosis and invasiveness and metastasis of tongue squamous cell carcinoma cell line Tca8113 cells].
abstract: OBJECTIVE
  To investigate the effect of downregulation of N-cadherin expression on cell proliferation, cell cycle, cell apoptosis and cell migration in tongue squamous cell carcinoma cell line Tca8113 cells.
  
  
  METHODS
  N-cadherin siRNA was transfected into tongue squamous cell carcinoma cell line Tca8113 cells and Tca8113 cells were divided into three groups: untreated group, control siRNA group and N-cadherin siRNA group. The cells were harvested 48 h after transfection with N-cadherin siRNA. Cell proliferation of Tca8113 cells was examined by cell counting kit (CCK)-8 after transfection with N-cadherin, and the effects of downregulation of N-cadherin on cell cycle and cell apoptosis of Tca8113 cells were investigated by flow cytometry. The effect of downregulation of N-cadherin expression on cell migration of Tca8113 cells was observed by Boyden chamber experiment, and further expression changes of gene-related cell proliferation, cell cycle and cell migration were detected by Western blotting.
  
  
  RESULTS
  N-cadherin siRNA downregulated the N-cadherin expression and significantly inhibited cell proliferation of Tca8113 cells (P < 0.05). The results of cell cycle revealed that the percentage of G(0)/G(1) phase in N-cadherin group [(65.41 ± 0.92)%] was significantly higher than that in untreated group [(41.59 ± 1.43)%] or control siRNA group [(43.70 ± 2.08)%], and there was significant difference among the three groups (F = 216.839, P = 0.000). The percentage of cell apoptosis in N-cadherin group [(25.66 ± 1.36)%] was significantly higher than that in untreated group [(2.38 ± 0.14)%] or control siRNA group [(2.81 ± 0.12)%], and there was significant difference among the three groups (F = 850.364, P = 0.000). The cell number migrated into memebrane in N-cadherin group was significantly lower than that in untreated group and control siRNA group, and there was significant difference among the three groups (F = 140.858, P = 0.000). Further, compared with untreated group and control siRNA group, the expressions of matrix metalloproteinase (MMP)-2 and MMP-9 proteins were significantly downregulated and expression of p21 protein was significantly upregulated (P < 0.05).
  
  
  CONCLUSIONS
  N-cadherin may play a role in occurrence and development of tongue squamous cell carcinoma.
authors.affiliation: Department of Stomatology, The First Affiliated Hospital of Zhengzhou University, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Jiao
mesh_terms: D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
chemical_list: D015820:Cadherins; D034741:RNA, Small Interfering; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D016283:Proto-Oncogene Proteins p21(ras)