title: Interaction of lactoferrin and lysozyme with casein micelles.
abstract: On addition of lactoferrin (LF) to skim milk, the turbidity decreases. The basic protein binds to the caseins in the casein micelles, which is then followed by a (partial) disintegration of the casein micelles. The amount of LF initially binding to casein micelles follows a Langmuir adsorption isotherm. The kinetics of the binding of LF could be described by first-order kinetics and similarly the disintegration kinetics. The disintegration was, however, about 10 times slower than the initial adsorption, which allowed investigating both phenomena. Kinetic data were also obtained from turbidity measurements, and all data could be described with one equation. The disintegration of the casein micelles was further characterized by an activation energy of 52 kJ/mol. The initial increase in hydrodynamic size of the casein micelles could be accounted for by assuming that it would go as the cube root of the mass using the adsorption and disintegration kinetics as determined from gel electrophoresis. The results show that LF binds to casein micelles and that subsequently the casein micelles partly disintegrate. All micelles behave in a similar manner as average particle size decreases. Lysozyme also bound to the casein micelles, and this binding followed a Langmuir adsorption isotherm. However, lysozyme did not cause the disintegration of the casein micelles.
authors.affiliation: Fonterra Research Centre, Private Bag 11029, Palmerston North, New Zealand.
authors.forename: Skelte G
authors.initials: SG
authors.lastname: Anema
authors.forename: C G Kees
authors.initials: CG
authors.lastname: de Kruif
mesh_terms: D000327:Adsorption; D000465:Algorithms; D000818:Animals; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D004527:Egg Proteins / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007781:Lactoferrin / Q000737:chemistry*; D008027:Light; D008823:Micelles; D008892:Milk / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry*; D009391:Nephelometry and Turbidimetry; D011485:Protein Binding; D012542:Scattering, Radiation
chemical_list: D002364:Caseins; D004527:Egg Proteins; D008823:Micelles; D009113:Muramidase; D007781:Lactoferrin