title: Diagnostic value of nonlinear optical signals from collagen matrix in the detection of epithelial precancer.
abstract: During precancer development in epithelium, neoplastic cells remodel the underlying stroma, for example, the basement membrane, capillaries, fibroblasts, and extracellular matrix. The purpose of this study is to investigate the relationship between the nonlinear optical signals from the collagen matrix in stroma and the progression of early epithelial carcinogenesis. Two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) signals were measured from the stroma of hamster oral cheek pouch in vivo. We found that three features, including the intensity ratio of TPEF over SHG, the spatial frequency distribution, and the texture feature of SHG images, provide the quantitative identification of epithelial precancer at different pathologic stages. We demonstrated that the combination of all three features by using a support vector machine algorithm can significantly improve the accuracy in the detection of epithelial precancer.
authors.affiliation: Biomedical Engineering Program, Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Hong Kong, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Shuxia
authors.initials: S
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Yang
authors.forename: Jianan Y
authors.initials: JY
authors.lastname: Qu
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism*; D006224:Cricetinae; D008297:Male; D009375:Neoplasms, Glandular and Epithelial / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D017711:Nonlinear Dynamics; D055620:Optical Phenomena; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D012372:ROC Curve
chemical_list: D003094:Collagen