title: Noninvasive imaging of ethanol-induced developmental defects in zebrafish embryos using optical coherence tomography.
abstract: In this article, we report the use of optical coherence tomography for noninvasive cross-sectional real-time imaging of ethanol-induced developmental defects in zebrafish embryos larvae. For ethanol concentration of over 300 mM, developmental defects of eye (shrinkage and retinal abnormalities), malformation of the notochord and ataxia arising due to the toxic effects of ethanol were observed in OCT images from 3 days post fertilization onwards. The results suggest that OCT could be a valuable tool for noninvasive assessment of birth defects in small animal systems.
authors.affiliation: Laser Biomedical Applications & Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore, India. kdivakar@rrcat.gov.in
authors.forename: K
authors.initials: K
authors.lastname: Divakar Rao
authors.forename: Piyush
authors.initials: P
authors.lastname: Upadhyaya
authors.forename: Mrinalini
authors.initials: M
authors.lastname: Sharma
authors.forename: P K
authors.initials: PK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000002:abnormalities* / Q000187:drug effects* / Q000473:pathology; D047108:Embryonic Development / Q000187:drug effects*; D000431:Ethanol / Q000633:toxicity*; D005123:Eye / Q000187:drug effects / Q000473:pathology; D005260:Female; D021621:Imaging, Three-Dimensional; D008297:Male; D016019:Survival Analysis; D013623:Tail / Q000002:abnormalities; D041623:Tomography, Optical Coherence / Q000379:methods*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D000431:Ethanol