title: Effect of instrumentation using curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser on the morphology and adhesion of blood components on root surfaces: a SEM study.
abstract: This study used scanning electron microscopy (SEM) to evaluate the morphology and adhesion of blood components on root surfaces instrumented by curettes, piezoelectric ultrasonic scaler and Er,Cr:YSGG laser. One hundred samples from 25 teeth were divided into 5 groups: 1) Curettes; 2) Piezoelectric ultrasonic scaler; 3) Curettes plus piezoelectric ultrasonic scaler; 4) Er,Cr:YSGG laser; 5) Curettes plus Er,Cr:YSGG laser. Ten samples from each group were used for analysis of root morphology and the other 10 were used for analysis of adhesion of blood components on root surface. The results were analyzed statistically by the Kruskall-Wallis and Mann-Whitney tests with a significance level of 5%. The group treated with curettes showed smoother surfaces when compared to the groups were instrumented with piezoelectric ultrasonic scaler and the Er,Cr:YSGG laser. The surfaces instrumented with piezoelectric ultrasonic scaler and Er,Cr:YSGG laser, alone or in combination with hand scaling and root planing, did not differ significantly (p>0.05) among themselves. No statistically significant differences (p>0.05) among groups were found as to the adhesion of blood components on root surface. Ultrasonic instrumentation and Er,Cr:YSGG irradiation produced rougher root surfaces than the use of curettes, but there were no differences among treatments with respect to the adhesion of blood components.
authors.affiliation: Araraquara Dental School, UNESP - Univ. Estadual Paulista, Araraquara, SP, Brazil.
authors.forename: Jackeline do Nascimento
authors.initials: Jdo N
authors.lastname: Tsurumaki
authors.forename: Bráulio Henrique Marques
authors.initials: BH
authors.lastname: Souto
authors.forename: Guilherme José Pimentel Lopes de
authors.initials: GJ
authors.lastname: Oliveira
authors.forename: José Eduardo Cézar
authors.initials: JE
authors.lastname: Sampaio
authors.forename: Elcio
authors.initials: E
authors.lastname: Marcantonio Júnior
authors.forename: Rosemary Adriana Chiérici
authors.initials: RA
authors.lastname: Marcantonio
mesh_terms: D001773:Blood Cells / Q000166:cytology*; D002448:Cell Adhesion / Q000502:physiology; D012534:Dental Scaling / Q000295:instrumentation* / Q000379:methods; D003804:Dentin / Q000528:radiation effects / Q000648:ultrastructure; D005337:Fibrin / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D053844:Lasers, Solid-State / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D059745:Piezosurgery / Q000295:instrumentation; D016745:Root Planing / Q000295:instrumentation* / Q000379:methods; D016369:Smear Layer; D013357:Subgingival Curettage / Q000295:instrumentation; D014092:Tooth Root / Q000528:radiation effects / Q000648:ultrastructure*
chemical_list: D005337:Fibrin