title: [Modeling of kidney collecting ducts function in the cell culture conditions].
abstract: Continuous cell lines originating from the kidney collecting duct represent a powerful tool for the modeling of water and ions reabsorbtion processes. Present review considers the basic methodical approaches being utilized to study vasopressin-induced water transport mechanisms in the cell culture conditions--microscopical methods, electrophysiological measurements, various ways of evaluation of water flow across the cell monolayer, transfections of native and mutant proteins, GFP-technology. The results of the highest significance for the understanding of collecting ducts function which were obtained with usage of these methods are analyzed in the review.
authors.forename: A N
authors.initials: AN
authors.lastname: Gorshkov
authors.forename: Ia Iu
authors.initials: IaIu
authors.lastname: Komissarchik
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D006801:Humans; D007685:Kidney Tubules, Collecting / Q000166:cytology / Q000502:physiology*; D008954:Models, Biological