title: Antibacterial activity of two biflavonoids from Garcinia livingstonei leaves against Mycobacterium smegmatis.
abstract: Amentoflavone and 4' monomethoxy amentoflavone were previously isolated from Garcinia livingstonei leaves. These compounds had good activities (MIC 6 and 8 μg/ml) against some nosocomial bacteria. In this study, the activity of these purified compounds were tested against fast-growing non-pathogenic Mycobacterium smegmatis. Amentoflavone was the most active compound, with an MIC of 0.60 ± 0.70 mg/ml. The MIC of 4' monomethoxy amentoflavone and the positive control isoniazid against Mycobacterium smegmatis were similar 1.40 ± 1.56 and 1.30 ± 1.70 mg/ml respectively. Although, Mycobacterium smegmatis is a non-pathogenic fast growing mycobacterium the activities of these compounds may also be useful in combating infections by pathogenic Mycobacterium spp.
authors.affiliation: Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. karkafa@yahoo.co.uk
authors.forename: A A
authors.initials: AA
authors.lastname: Kaikabo
authors.forename: J N
authors.initials: JN
authors.lastname: Eloff
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D044946:Biflavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D029760:Garcinia / Q000737:chemistry*; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D020102:Mycobacterium smegmatis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves
chemical_list: D000900:Anti-Bacterial Agents; D044946:Biflavonoids; D010936:Plant Extracts; C011164:amentoflavone; D007538:Isoniazid