title: Activity of β2-adrenergic receptor in oral squamous cell carcinoma is mediated by overexpression of the ADRBK2 gene: a pilot study.
abstract: The β2-adrenergic receptor is most frequently involved in carcinogenic processes. Earlier studies have established a relation between the β2-adrenergic receptor and various characteristics of cancer including cell proliferation, apoptosis, chemotaxis, metastasis, tumor growth and angiogenesis. Our goal was to determine differential expression of the genes involved in adrenergic receptors using DNA microarrays and to confirm their under- or overexpression using real-time quantitative PCR. Five of the nine genes investigated showed significantly altered expression levels in tumor cells (p < 0.05). The gene product with the highest Z-score (restrictive statistical technique for selection of appropriate genes to study) was ADRBK2. Significantly, most of the overexpressed genes were related to β-adrenergic receptors. Real-time PCR analysis confirmed the up regulation observed in the microarrays, which indicated overexpression in 100% of the tumors. In oral squamous cell carcinomas, malignant cells and surrounding tissue overexpress the ADRBK2 gene.
authors.affiliation: Oral Medicine, Oral Surgery and Implantology Unit, Faculty of Medicine and Dentistry, Entrerríos s/n, Santiago de Compostela, Spain. perezsayans@gmail.com
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Sayáns
authors.forename: J M
authors.initials: JM
authors.lastname: Somoza-Martín
authors.forename: F
authors.initials: F
authors.lastname: Barros-Angueira
authors.forename: P
authors.initials: P
authors.lastname: Gayoso-Diz
authors.forename: E M
authors.initials: EM
authors.lastname: Otero-Rey
authors.forename: J M
authors.initials: JM
authors.lastname: Gándra-Rey
authors.forename: A
authors.initials: A
authors.lastname: García-García
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D054770:G-Protein-Coupled Receptor Kinase 3 / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D009062:Mouth Neoplasms / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D010865:Pilot Projects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D018343:Receptors, Adrenergic, beta-2; D054770:G-Protein-Coupled Receptor Kinase 3; C517848:GRK3 protein, human