title: [Count and function of CD8(+)CXCR3(+) regulatory T cells in peripheral blood of patients with autoimmune hemolytic anemia].
abstract: OBJECTIVE
  To explore the effects of CD8(+)CXCR3(+)T cells on autoimmune hemolytic anemia (AIHA).
  
  
  METHODS
  Twenty-two AIHA patients, including 11 untreated and 11 recovered ones, and 23 normal controls were recruited from July 2010 to November 2010. The percentage of CD8(+)CXCR3(+)/CD8(+)T cells in peripheral blood and the expression of interleukin-10 (IL-10) in CD8(+)CXCR3(+)T cells were detected by flow cytometry. Their correlations with the count of CD3(+)CD4(+)cells and the percentage of CD5(+)CD19(+) in CD19(+) B cells were analyzed. The expression level of CXCR3 mRNA in PBMC was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The percentage of CD8(+)CXCR3(+)/CD8(+) of untreated AIHA patients was (39.80 ± 19.96)%. And it was lower than that of recovered patients [(58.76 ± 14.22)%, P < 0.05] and normal controls [(59.66 ± 12.62)%, P < 0.01]. The percentage of IL-10(+) T cells in CD8(+)CXCR3(+)T cells of untreated patients was (22.98 ± 14.96)% and it was lower than that of normal controls [(38.15 ± 17.03)%, P < 0.05]. The expression level of CXCR3 mRNA for untreated AIHA patients was (0.51 ± 0.19) and it was lower than that of normal controls (1.67 ± 1.17, P < 0.01). The percentage of CD8(+)CXCR3(+)/CD8(+)T cells had a negative correlation with the count of CD3(+)CD4(+) cells and the percentage of CD5(+)CD19(+)/CD19(+) B cells (r = -0.571, -0.583, both P < 0.05). So did the percentage of IL-10(+) T cells in CD8(+)CXCR3(+)T cells (r = -0.524, -0.523, both P < 0.05).
  
  
  CONCLUSIONS
  The decreased count of CD8(+)CXCR3(+)T cells and the lowered level of IL-10 may disturb the immune tolerance and lead to the occurrence of AIHA.
authors.affiliation: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Qian-na
authors.initials: QN
authors.lastname: Zhou
authors.forename: Li-min
authors.initials: LM
authors.lastname: Xing
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hua-quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Er-bao
authors.initials: EB
authors.lastname: Ruan
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Zong-hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000328:Adult; D000368:Aged; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D054367:Receptors, CXCR3 / Q000276:immunology
chemical_list: C515187:CXCR3 protein, human; C508609:IL10 protein, human; D054367:Receptors, CXCR3; D016753:Interleukin-10