title: Anti-Mullerian hormone as a predictive endocrine marker for embryo production in the goat.
abstract: Recently, we demonstrated the relationship between anti-Müllerian hormone (AMH) circulating concentrations, ovarian follicles, and embryo production in cattle. However, they have not yet been established in a species with a seasonal breeding activity. Thus, goats were subjected to repeated in vivo embryo production during the breeding season, at the end of the breeding season, and at the end of the anestrus season. Embryo production after FSH treatment was highly repeatable for each goat. Plasma AMH concentrations, measured before the first FSH treatment, were highly correlated with the number of collected, transferable, and freezable embryos, resulting from the three sessions of embryo production. Plasma AMH concentrations transiently decreased after each exogenous FSH treatment, but they showed little change with season, and no relationship was observed between AMH and endogenous FSH concentrations during seasonal transitions. Follicles of 1-5 mm in diameter were the main target of the FSH treatment and were major contributors to circulating AMH concentrations. Granulosa cell AMH expression decreased as the follicle approached terminal development, while the expression of maturation markers (CYP19A1 and FSHR) increased. In conclusion, circulating AMH concentrations can be predictive of the capacity of a donor goat to produce high or low numbers of high-quality embryos. This prediction could be accurately made from a single blood measurement of AMH during either breeding or anestrus seasons. Variability in the number of gonadotropin-responsive follicles of 1-5 mm in diameter between individuals resulted in the differences in circulating AMH concentrations measured between individuals.
authors.affiliation: Physiologie de la Reproduction et des Comportements, UMR 085 INRA-UMR 6175 CNRS-Université de Tours-IFCE, Centre INRA de Tours, 37380 Nouzilly, France. dmonniaux@tours.inra.fr
authors.forename: Danielle
authors.initials: D
authors.lastname: Monniaux
authors.forename: Gérard
authors.initials: G
authors.lastname: Baril
authors.forename: Anne-Lyse
authors.initials: AL
authors.lastname: Laine
authors.forename: Peggy
authors.initials: P
authors.lastname: Jarrier
authors.forename: Natividad
authors.initials: N
authors.lastname: Poulin
authors.forename: Juliette
authors.initials: J
authors.lastname: Cognié
authors.forename: Stéphane
authors.initials: S
authors.lastname: Fabre
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood* / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D004622:Embryo, Mammalian; D047108:Embryonic Development; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006041:Goats / Q000097:blood*; D006080:Ovarian Follicle / Q000378:metabolism; D010062:Ovulation Induction; D012333:RNA, Messenger / Q000378:metabolism; D012621:Seasons
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone