title: Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer.
abstract: BACKGROUND
  Newcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds but causes only mild flulike symptoms in human beings. NDV(F3aa)-GFP is a genetically modified, fusogenic NDV. We assessed the utility of NDV(F3aa)-GFP in treating head and neck squamous cell carcinoma.
  
  
  RESULTS
  At a multiplicity of infection (MOI) of 1, NDV(F3aa)-GFP infection of 3 cell lines supported strong GFP expression by 36 hours. Four cell lines were highly sensitivite to viral cytotoxicity, with >75% of cells lysed by day 6 at MOI 0.1, and 2 other cell lines were partially susceptible. Murine SCC25 flank tumors exhibited robust GFP expression after a single intratumoral viral injection and showed near-complete tumor regression over 34 days. There were no adverse effects attributable to therapy.
  
  
  CONCLUSIONS
  We demonstrate that a fusogenic NDV exerts potent oncolytic effects against human head and neck cancer and support its continued investigation for clinical application.
authors.affiliation: Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
authors.forename: Pingdong
authors.initials: P
authors.lastname: Li
authors.forename: Chun-Hao
authors.initials: CH
authors.lastname: Chen
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.forename: Babak
authors.initials: B
authors.lastname: Givi
authors.forename: Zhenkun
authors.initials: Z
authors.lastname: Yu
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Zamarin
authors.forename: Peter
authors.initials: P
authors.lastname: Palese
authors.forename: Yuman
authors.initials: Y
authors.lastname: Fong
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Wong
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000628:therapy*; D045744:Cell Line, Tumor; D005260:Female; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D006258:Head and Neck Neoplasms / Q000628:therapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009522:Newcastle disease virus / Q000235:genetics*; D050130:Oncolytic Virotherapy / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins