title: Association of angiotensin converting enzyme (ACE) gene I/D polymorphism and polycystic ovary syndrome (PCOS).
abstract: This study was conducted in Turkish patients with polycystic ovary syndrome to determine the frequency of I/D polymorphism genotypes of angiotensin converting enzyme gene, and to examine the role of this polymorphism in polycystic ovary syndrome development. Genomic DNA obtained from 200 persons (100 patients with polycystic ovary syndrome and 100 healthy controls) was used in the study. DNA was multiplied by polymerase chain reaction using I and D allele-specific primers. Polymerase chain reaction products were assessed with a charge coupled device (CCD) camera by being exposed to 2% agarose gel electrophoresis. There was statistically significant difference between the groups with respect to genotype distribution (p<0.001). The D allele frequency was indicated as 68% and I allele was as 32% in the patients, whereas it was 51.5-48.5% respectively in the control group. As a result of our study we may assert that angiotensin converting enzyme gene I/D polymorphism DD genotype should be considered as a genetic marker in polycystic ovary syndrome development in this Turkish study population.
authors.affiliation: Muş Alparslan University, Faculty of Arts and Science, Department of Biology, Turkey. bayrambanu@hotmail.com
authors.forename: Banu
authors.initials: B
authors.lastname: Bayram
authors.forename: Cetin
authors.initials: C
authors.lastname: Kılıççı
authors.forename: Harun
authors.initials: H
authors.lastname: Onlü
authors.forename: Mete
authors.initials: M
authors.lastname: Ozkurt
authors.forename: Nilüfer
authors.initials: N
authors.lastname: Erkasap
authors.forename: Engin
authors.initials: E
authors.lastname: Yıldırım
authors.forename: Fezan
authors.initials: F
authors.lastname: Sahin
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D014421:Turkey
chemical_list: D005819:Genetic Markers; D007703:Peptidyl-Dipeptidase A