title: Metabolically stable cellular adhesion to inert surfaces.
abstract: The structure of D-amino acid hexapeptides that promote cellular adhesion was determined by screening D-amino acid hexapeptide libraries synthesized on otherwise inert beaded PEGA resin. These new adhesion molecules provide a completely stable cellular environment and facilitate the maintenance of a monolayer of cells on beads for extended periods. The presence of the peptides promotes spreading of the cells on the bead surface. Not surprisingly, the molecules contained a significant number of arginines and/or lysines. However, the exact structure of each peptide is quite important for the degree of adhesion observed, and a motif with three or four basic amino acids spaced within amino acids of intermediate polarity clearly prevailed, for example, k-l/r-h-r-i/v-r-a; this maintains a polar/hydrophobic balance.
authors.affiliation: Nano Science Center, University of Copenhagen, Copenhagen, Denmark. meldal@nano.ku.dk
authors.forename: Morten
authors.initials: M
authors.lastname: Meldal
authors.forename: Boqian
authors.initials: B
authors.lastname: Wu
authors.forename: Frederik
authors.initials: F
authors.lastname: Diness
authors.forename: Roice
authors.initials: R
authors.lastname: Michael
authors.forename: Grith
authors.initials: G
authors.lastname: Hagel
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002448:Cell Adhesion; D002460:Cell Line; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D056004:Molecular Dynamics Simulation; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D019151:Peptide Library; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D000596:Amino Acids; D009842:Oligopeptides; D019151:Peptide Library; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D011092:Polyethylene Glycols