title: Effect of autologous bone marrow-derived cells associated with guided bone regeneration or not in the treatment of peri-implant defects.
abstract: This study investigated the effect of bone marrow-derived cells associated with guided bone regeneration in the treatment of dehiscence bone defects around dental implants. Iliac-derived bone marrow cells were harvested from dogs and phenotypically characterized with regard to their osteogenic properties. After teeth extraction, three implant sites were drilled, dehiscences created and implants placed. Dehiscences were randomly assigned to: bone marrow-derived cells, bone marrow-derived cells+guided bone regeneration, and control (no treatment). After 3 months, implants with adjacent tissues were processed histologically, bone-to-implant contact, bone fill within the threads, new bone area in a zone lateral to the implant, new bone height, and new bone weight at the bottom of the defect were determined. Phenotypic characterization demonstrated that bone marrow-derived cells presented osteogenic potential. Statistically higher bone fill within the threads was observed in both bone marrow-derived cells+guided bone regeneration bone marrow-derived cell groups compared with the control group (P<0.05), with no difference between the groups treated with cells (P>0.05). For the other parameters (new bone area, bone-to-implant contact, new bone height and new bone weight), only the bone marrow-derived cells+guided bone regeneration group presented higher values compared with the non-treated control (P<0.05). Bone marrow-derived cells provided promising results for peri-implantar bone regeneration, although the combined approach seems to be relevant, especially to bone formation out of the implant threads.
authors.affiliation: Department of Prosthodontics and Periodontics, School of Dentistry at Piracicaba, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil.
authors.forename: F V
authors.initials: FV
authors.lastname: Ribeiro
authors.forename: F F
authors.initials: FF
authors.lastname: Suaid
authors.forename: K G S
authors.initials: KG
authors.lastname: Ruiz
authors.forename: T L
authors.initials: TL
authors.lastname: Rodrigues
authors.forename: M D
authors.initials: MD
authors.lastname: Carvalho
authors.forename: F H
authors.initials: FH
authors.lastname: Nociti
authors.forename: E A
authors.initials: EA
authors.lastname: Sallum
authors.forename: M Z
authors.initials: MZ
authors.lastname: Casati
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery*; D000539:Alveolar Process / Q000473:pathology; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D048430:Cell Shape; D024042:Collagen Type I / Q000032:analysis; D015921:Dental Implants; D004285:Dogs; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods*; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010641:Phenotype; D011138:Polytetrafluoroethylene; D011897:Random Allocation; D013997:Time Factors; D054457:Tissue Scaffolds; D014025:Titanium; D020390:Tooth Socket / Q000473:pathology / Q000601:surgery; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D024042:Collagen Type I; D015921:Dental Implants; D058574:Integrin-Binding Sialoprotein; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; D014025:Titanium; D000469:Alkaline Phosphatase