title: Surface interactions of monoclonal antibodies characterized by quartz crystal microbalance with dissipation: impact of hydrophobicity and protein self-interactions.
abstract: Surface adsorption of two monoclonal antibodies (mAb1 and mAb2), with widely different hydrophobicity and self-association behavior in solution, was examined by quartz crystal microbalance with dissipation monitoring to understand how adsorption and protein self-interactions near the surface are impacted by their intrinsic properties. The dependence of mass and viscoelastic properties of the adsorbed protein layer on the type of surface, presence of a surfactant, protein concentration, and pH were examined. Adsorption was significantly reduced in the presence of surfactant for both proteins, but for the more hydrophobic mAb2, residual adsorption remained on polystyrene (PS) and Teflon surfaces. Protein concentration had little impact on the adsorbed protein mass for silicon dioxide surface but had a significant impact for PS and Teflon surfaces. At high protein concentrations, an irreversible layer formed first upon which a reversible layer builds. Reversible adsorption was significantly greater at higher protein concentrations and significantly higher for mAb2, consistent with its higher propensity to reversibly self-associate in solution. The viscoelastic properties suggest that adsorbed protein layer at high protein concentrations is more hydrated. The adsorbed protein layer at lower pH was more hydrated, and possibly more unfolded, consistent with the behavior of the antibody in bulk solution.
authors.affiliation: Biolin Scientific AB/Q-Sense, SE-426 77 Västra Frölunda, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Oom
authors.forename: Mark
authors.initials: M
authors.lastname: Poggi
authors.forename: Jennie
authors.initials: J
authors.lastname: Wikström
authors.forename: Muppalla
authors.initials: M
authors.lastname: Sukumar
mesh_terms: D000327:Adsorption; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004548:Elasticity; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D011791:Quartz; D014783:Viscosity
chemical_list: D000911:Antibodies, Monoclonal; D011506:Proteins; D011791:Quartz