title: Antiplatelet, Antithrombotic, and Fibrinolytic Activities of Campomanesia xanthocarpa.
abstract: In a previous work based on popular belief, Campomanesia xanthocarpa Berg., popularly known as "guavirova", showed to have a potential effect in the control of a number of conditions associated with cardiovascular diseases. The aim of the present work was to investigate the effects of C. xanthocarpa extract (CXE) on antiplatelet, antithrombotic and fibrinolytic activities in mice and in human blood. Mice were treated orally for 5 days with CXE or acetylsalicylic acid and at the end of the treatment period animals were challenged for bleeding, acute thromboembolism and ulcerogenic activity. In addition, we have assessed the prothrombin time and activated partial thromboplastin time (aPTT) after oral administration. In in vitro assays, antiplatelet effects of CXE was evaluated on platelet aggregation, and fibrinolytic activity of the extract was observed by mice or human artificial blood clot degradation. Platelet citotoxicity of the extract was also determined by the LDH assay. Results demonstrated that CXE has a significant protective effect on thrombosis. It also inhibits platelet aggregation without demonstrating cytotoxicity on platelets. CXE slightly prolonged aPTT and showed no ulcerogenic activity after oral administration. In addition, CXE showed a fibrinolytic activity. Thus, C. xanthocarpa showed antiplatelet, antithrombotic and fibrinolytic activities in mice.
authors.affiliation: Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Jonatas Zeni
authors.initials: JZ
authors.lastname: Klafke
authors.forename: Mariane
authors.initials: M
authors.lastname: Arnoldi da Silva
authors.forename: Mateus
authors.initials: M
authors.lastname: Fortes Rossato
authors.forename: Gabriela
authors.initials: G
authors.lastname: Trevisan
authors.forename: Cristiani Isabel
authors.initials: CI
authors.lastname: Banderó Walker
authors.forename: Cláudio Alberto
authors.initials: CA
authors.lastname: Martins Leal
authors.forename: Diego
authors.initials: D
authors.lastname: Olschowsky Borges
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Chitolina Schetinger
authors.forename: Rafael
authors.initials: R
authors.lastname: Noal Moresco
authors.forename: Marta Maria
authors.initials: MM
authors.lastname: Medeiros Frescura Duarte
authors.forename: Adair Roberto
authors.initials: AR
authors.lastname: Soares Dos Santos
authors.forename: Paulo Ricardo
authors.initials: PR
authors.lastname: Nazário Viecili
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira