title: Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood.
abstract: BACKGROUND
  We have shown that approximately 30% of human peripheral blood B-cells express CD25. B cells expressing CD25 display a mature phenotype belonging to the memory B-cell population and have a better proliferative and antigen-presenting capacity. The aim of the present study was to characterize the CD25-expressing subset of B cells in human cord blood.
  
  
  METHODS
  Mononuclear cell fraction from human cord blood (n=34) and peripheral adult blood (n=22) was sorted into CD20+CD25+ and CD20+CD25- B-cell populations. Phenotype and function of these B-cell populations were compared using flow cytometry, proliferation, cytokine production, and immunoglobulin secretion.
  
  
  RESULTS
  CD25-expressing B cells are a limited population of cord blood mononuclear cells representing 5% of the CD20+ B cells. They are characterised by high expression of CD5 in cord blood and CD27 in adult blood. CD25-expressing B cells express a functional IL-2 receptor and high levels of CC-chemokine receptors and spontaneously produce antibodies of IgG and IgM subclass.
  
  
  CONCLUSIONS
  CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation, possibly ready to migrate and home into the peripheral tissue for further differentiation/action.
authors.affiliation: Department of Rheumatology and Inflammation Research, Sahlgrenska Academy at Göteborg University, P.O. Box 480, 405 30 Göteborg, Sweden.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Amu
authors.forename: Mikael
authors.initials: M
authors.lastname: Brisslert
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism*; D008875:Middle Aged; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018956:CD5 Antigens; D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D053645:Interleukin-2 Receptor alpha Subunit; D011947:Receptors, Antigen, B-Cell; D015375:Receptors, Interleukin-2; D016201:Receptors, Lymphocyte Homing; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7