title: Effect of ascorbic acid-rich diet on in vivo-induced oxidative stress.
abstract: Using hyperbaric oxygen (HBO) therapy as an in vivo oxidation model, we investigated the effect of a diet enriched in ascorbic acid (AA) on HBO-induced oxidative stress. Volunteers (n 46) were allocated to the AA-rich diet group or the control group. Blood samples were collected at the basal time, after the 1-week diet before and immediately after the HBO treatment, and 1 week after the HBO treatment. AA level, total antioxidant status (TAS), hydroperoxides (HP), lymphocyte DNA oxidation and DNA repair capacity were assessed. The expression of genes involved in oxidative stress was evaluated in lymphocytes and the protein activity of the modulated genes was determined in the plasma. The AA level and the antioxidant status of plasma were increased by AA-rich food consumption. HBO exposure did not affect the AA levels or TAS, but induced HP formation in the control group. The lymphocytes isolated from dietary-supplemented subjects were resistant to ex vivo DNA oxidation, showing an increased DNA repair capacity compared with controls. A difference in gene expression pattern was observed between the groups. AA-rich foods provide dual protection against oxidative stress, enhancing plasma antioxidant levels and stimulating genes involved in cell detoxification.
authors.affiliation: Department of Anesthesiology Research Unit, IRCCS Rizzoli Orthopaedic Institute, Via Pupilli, 1-40136 Bologna, Italy. rena.alleva@gmail.com
authors.forename: Renata
authors.initials: R
authors.lastname: Alleva
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Di Donato
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Strafella
authors.forename: Sara
authors.initials: S
authors.lastname: Staffolani
authors.forename: Linda
authors.initials: L
authors.lastname: Nocchi
authors.forename: Battista
authors.initials: B
authors.lastname: Borghi
authors.forename: Elettra
authors.initials: E
authors.lastname: Pignotti
authors.forename: Lory
authors.initials: L
authors.lastname: Santarelli
authors.forename: Marco
authors.initials: M
authors.lastname: Tomasetti
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis; D001205:Ascorbic Acid / Q000008:administration & dosage* / Q000097:blood; D004249:DNA Damage; D004260:DNA Repair; D004032:Diet; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D005979:Glutathione Peroxidase / Q000097:blood / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000097:blood / Q000235:genetics / Q000378:metabolism; D006801:Humans; D006931:Hyperbaric Oxygenation / Q000009:adverse effects; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010545:Peroxides / Q000097:blood
chemical_list: D000975:Antioxidants; D010545:Peroxides; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D001205:Ascorbic Acid