title: Feasibility study on retinal vascular bypass surgery in isolated arterially perfused caprine eye model.
abstract: OBJECTIVE
  To investigate the feasibility of bypassing occluded segments of retinal venous main vessels in isolated, arterially perfused caprine eyes via the closed-sky vitrectomy approach using keratoprosthesis.
  
  
  METHODS
  Isolated caprine eyes were used in this study. For each eye, the retinal vessel was perfused by Krebs solution via ophthalmic artery, and pars plana vitrectomy was performed using temporary keratoprosthesis. All retinal micro-vascular maneuvers were performed in a closed-sky eyeball. The main retinal vein was blocked by endodiathermy at the site of the vessel's first branching. Two openings, several millimeters apart, were created by vascular punctures in both the main vein and its branch vein wall straddling the induced occluded segment. Catheterization was achieved using a flexible polyimide tube, with each end inserted into the vessel wall opening. A sealed connection between the vessel and the tube was obtained by endodiathermy. Bypass of the occluded retinal vein segment was thus achieved, and the patency of this vascular bypass was confirmed by intravascular staining.
  
  
  RESULTS
  Puncturing, catheterization, and endodiathermy were viable by closed-sky approach using keratoprosthesis. Bypassing of the occluded retinal main vein segment was accomplished with the combination of these maneuvers. Good results were obtained in 23 of 38 (60%) caprine eyes.
  
  
  CONCLUSIONS
  This study demonstrated that bypassing the occluded segment of retinal main vein can be successfully performed in a closed-sky eyeball model of isolated, arterially perfused caprine eye. This early work indicated that the more advanced retinal vascular bypass surgery in in vivo eye may be feasible in the future.
authors.affiliation: Eye Hospital of Wenzhou Medical College, Wen Zhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Fan
authors.forename: L
authors.initials: L
authors.lastname: Shen
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000379:methods*; D002404:Catheterization / Q000379:methods; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006041:Goats; D008866:Microsurgery / Q000379:methods; D013508:Ophthalmologic Surgical Procedures; D012170:Retinal Vein Occlusion / Q000503:physiopathology / Q000601:surgery*; D012171:Retinal Vessels / Q000601:surgery; D014821:Vitrectomy / Q000379:methods*