title: Reconstructing an icosahedral virus from single-particle diffraction experiments.
abstract: The first experimental data from single-particle scattering experiments from free electron lasers (FELs) are now becoming available. The first such experiments are being performed on relatively large objects such as viruses, which produce relatively low-resolution, low-noise diffraction patterns in so-called "diffract-and-destroy" experiments. We describe a very simple test on the angular correlations of measured diffraction data to determine if the scattering is from an icosahedral particle. If this is confirmed, the efficient algorithm proposed can then combine diffraction data from multiple shots of particles in random unknown orientations to generate a full 3D image of the icosahedral particle. We demonstrate this with a simulation for the satellite tobacco necrosis virus (STNV), the atomic coordinates of whose asymmetric unit is given in Protein Data Bank entry 2BUK.
authors.affiliation: Department of Physics, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, USA. dksaldin@uwm.edu
authors.forename: D K
authors.initials: DK
authors.lastname: Saldin
authors.forename: H-C
authors.initials: HC
authors.lastname: Poon
authors.forename: P
authors.initials: P
authors.lastname: Schwander
authors.forename: M
authors.initials: M
authors.lastname: Uddin
authors.forename: M
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000465:Algorithms; D021621:Imaging, Three-Dimensional / Q000379:methods* / Q000706:statistics & numerical data; D007834:Lasers; D055620:Optical Phenomena; D012542:Scattering, Radiation; D025641:Tobacco necrosis satellite virus / Q000648:ultrastructure*; D014961:X-Ray Diffraction