title: [Diversity analysis of desulfuration bacterium from the oxidation ditch of city sewage treatment plant with SO2 gas].
abstract: A group of removing SO2 bacterium was obtained from the oxidation ditch of city sewage treatment plant by inductive domestication over 6 d with low concentration SO2 gas, and they have an ability with biodegradation rate of 888 mg x (L x h)(-1) and a degradation efficiency of 85% during 1.5 h for SO2 dissolved in water with their synergy. The clone library and two phylogenetic trees of the removing SO2 bacterium communities were obtained based on 16S rRNA DNA comparison by DNA extraction of the sample and in situ polymerase chain reaction (PCR). The phylogenetic analysis showed that 8 dominant desulfuration bacterium occupy about 69% of all removing SO2 bacterium, and some of them have a kindred with discovered desulfuration bacterium but not homogeneity, and there are four belong to alpha-Proteobacteria, another four belong to beta-Proteobacteria in them. The gene information about 16S rRNA sequence of the dominant desulfuration bacteria and domestication method provide a basic of looking for or domesticating removing SO2 bacterium for development microbial desulfurization technology of contained SO2 tail gas.
authors.affiliation: Faculty of Environmental Science & Technology, Kunming University of Science and Technology, Kunming 650093, China. kmhuangbing@yahoo.com.cn
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.forename: Shi-Ling
authors.initials: SL
authors.lastname: Zhang
authors.forename: Jiang-Hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Shi
mesh_terms: D044822:Biodiversity; D002947:Cities; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013458:Sulfur Dioxide / Q000737:chemistry*; D016999:Sulfur-Reducing Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D012336:RNA, Ribosomal, 16S; D012722:Sewage; D013458:Sulfur Dioxide