title: R-flurbiprofen, a novel nonsteroidal anti-inflammatory drug, decreases cell proliferation and induces apoptosis in pituitary adenoma cells in vitro.
abstract: R-flurbiprofen, a nonsteroidal anti-inflammatory drug derivative, has been shown to inhibit colonic adenoma formation in mice. We investigated the effects of R-flurbiprofen on cell proliferation and apoptosis in pituitary adenoma cell lines. GH4C1 rat pituitary cell line cultures and low-passage human primary pituitary cell cultures were treated with varying concentrations of R-flurbiprofen (0.1-1.0 mM). R-flurbiprofen inhibited cell proliferation in a dose-dependent fashion. A terminal deoxynucleotidyl transferase dUTP nick end labeling assay and chromatin condensation/dead cell apoptosis assay demonstrated induction of apoptosis at higher concentrations of R-flurbiprofen. R-flurbiprofen decreases cell proliferation and induces apoptosis in pituitary adenoma cells in vitro. This may be a potential therapy in the management of pituitary adenoma.
authors.affiliation: Department of Neurosurgery, Huntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT, USA. james.liu@umdnj.edu
authors.forename: James K
authors.initials: JK
authors.lastname: Liu
authors.forename: Smruti K
authors.initials: SK
authors.lastname: Patel
authors.forename: David L
authors.initials: DL
authors.lastname: Gillespie
authors.forename: Kum
authors.initials: K
authors.lastname: Whang
authors.forename: William T
authors.initials: WT
authors.lastname: Couldwell
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003851:Deoxyribonucleases; D004305:Dose-Response Relationship, Drug; D005480:Flurbiprofen / Q000494:pharmacology*; D006801:Humans; D020287:In Situ Nick-End Labeling; D010911:Pituitary Neoplasms / Q000473:pathology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005480:Flurbiprofen; D003851:Deoxyribonucleases