title: n-3 PUFA prevent metabolic disturbances associated with obesity and improve endothelial function in golden Syrian hamsters fed with a high-fat diet.
abstract: Glucose intolerance and dyslipidaemia are independent risk factors for endothelium dysfunction and CVD. The aim of the present study was to analyse the preventive effect of n-3 PUFA (EPA and DHA) on lipid and carbohydrate disturbances and endothelial dysfunction. Three groups of adult hamsters were studied for 20 weeks: (1) control diet (Control); (2) high-fat diet (HF); (3) high-fat diet enriched with n-3 PUFA (HFn-3) groups. The increase in body weight and fat mass in the HF compared to the Control group (P < 0.05) was not found in the HFn-3 group. Muscle TAG content was similar in the Control and HF groups, but significantly lower in the HFn-3 group (P = 0.008). Glucose tolerance was impaired in the HF compared to the Control group, but this impairment was prevented by n-3 PUFA in the HFn-3 group (P < 0.001). Plasma TAG and cholesterol were higher in the HF group compared to the Control group (P < 0.001), but lower in the HFn-3 group compared to the HF group (P < 0.001). HDL-cholesterol was lower in the HFn-3 group compared to the Control and HF groups (P < 0.0005). Hepatic secretion of TAG was lower in the HFn-3 group compared to the HF group (P < 0.005), but did not differ from the Control group. Hepatic gene expression of sterol regulatory element-binding protein-1c, diacylglycerol O-acyltransferase 2 and stearyl CoA desaturase 1 was lower in the HFn-3 group, whereas carnitine palmitoyl transferase 1 and scavenger receptor class B type 1 expression was higher (P < 0.05). In adipocytes and adipose macrophages, PPARγ and TNFα expression was higher in the HF and HFn-3 groups compared to the Control group. Endothelium relaxation was higher in the HFn-3 (P < 0.001) than in the HF and Control groups, and was correlated with glucose intolerance (P = 0.03) and cholesterol (P = 0.0003). In conclusion, n-3 PUFA prevent some metabolic disturbances induced by high-fat diet and improve endothelial function in hamsters.
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Fatima
authors.initials: F
authors.lastname: Kasbi Chadli
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Andre
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Prieur
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Gervaise
authors.initials: G
authors.lastname: Loirand
authors.affiliation: INRA, UR1368, Biopolymeres Interactions Assemblies, Rue de La Géraudière, BP 71627, 44316 Nantes, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Meynier
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Krempf
authors.affiliation: L'UNAM Oniris, Nutrition and Endocrinology Unit, National College of Veterinary Medicine, Food Science and Engineering, Nantes F-44307, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Nguyen
authors.affiliation: INSERM, UMR 915, IRT - UN L'institut du thorax, 8 quai Moncousu, BP 70721, 44007 Nantes Cedex 1, France.
authors.forename: Khadija
authors.initials: K
authors.lastname: Ouguerram
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D001011:Aorta / Q000473:pathology; D001823:Body Composition; D001835:Body Weight; D018955:CD36 Antigens / Q000378:metabolism; D050260:Carbohydrate Metabolism; D006224:Cricetinae; D059305:Diet, High-Fat; D050171:Dyslipidemias / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D018149:Glucose Intolerance; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000166:cytology; D008297:Male; D008647:Mesocricetus; D009132:Muscles / Q000378:metabolism; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D018955:CD36 Antigens; D015525:Fatty Acids, Omega-3; D008055:Lipids; D008079:Lipoproteins, VLDL; D005947:Glucose