title: A high-throughput gateway-compatible yeast one-hybrid screen to detect protein-DNA interactions.
abstract: In recent years, new techniques have spurred the discovery of cis-regulatory DNA elements. These stretches of noncoding DNA contain combinations of recognition sites to which transcription factors (TFs) bind, and in doing so, these TFs can activate or repress transcription. These protein-DNA interactions form the core of gene regulatory networks (GRNs) that are responsible for the differential gene expression that allow diversification of cell types, developmental programs, and responses to the environment. The yeast one-hybrid system is a genetic assay to identify direct binding of proteins to DNA elements of interest and is, therefore, instrumental in uncovering these GRNs.
authors.affiliation: Laboratory of Systems Biology and Genetics, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
authors.forename: Korneel
authors.initials: K
authors.lastname: Hens
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Feuz
authors.forename: Bart
authors.initials: B
authors.lastname: Deplancke
mesh_terms: D004247:DNA / Q000235:genetics* / Q000378:metabolism*; D053263:Gene Regulatory Networks / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D011485:Protein Binding; D050436:Regulatory Elements, Transcriptional / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D014157:Transcription Factors; D004247:DNA