title: Intracellular reactive oxygen species production by polymorphonuclear leukocytes in bovine leukemia virus-infected dairy cows.
abstract: The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. aureus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Cidade Universitária, São Paulo, SP 05508–270, Brazil. nogueirasouza@yahoo.com.br
authors.forename: Fernando Nogueira de
authors.initials: FN
authors.lastname: Souza
authors.forename: Maiara Garcia
authors.initials: MG
authors.lastname: Blagitz
authors.forename: Andréia Oliveira
authors.initials: AO
authors.lastname: Latorre
authors.forename: Eduardo Milton
authors.initials: EM
authors.lastname: Ramos Sanchez
authors.forename: Camila Freitas
authors.initials: CF
authors.lastname: Batista
authors.forename: Rebeca Alves
authors.initials: RA
authors.lastname: Weigel
authors.forename: Francisco Palma
authors.initials: FP
authors.lastname: Renno
authors.forename: Maria Claudia Araripe
authors.initials: MC
authors.lastname: Sucupira
authors.forename: Alice Maria Melville Paiva
authors.initials: AM
authors.lastname: Della Libera
mesh_terms: D000818:Animals; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000097:blood* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D009504:Neutrophils / Q000378:metabolism*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016897:Respiratory Burst / Q000276:immunology; D018709:Statistics, Nonparametric
chemical_list: D017382:Reactive Oxygen Species