title: Strain dependent expression of stress response and virulence genes of Listeria monocytogenes in meat juices as determined by microarray.
abstract: A subgenomic array, encompassing 54 probes targeting genes responsible for virulence, adhesion and stress response in Listeria monocytogenes, was used in order to study their expression in food systems. RNA extracted from L. monocytogenes inoculated in BHI and in situ (i.e. in minced meat and fermented sausage juices) and incubated at 4°C, was hybridized on the array and the results obtained were compared in order to understand the effect that the food juice has on the expression. Three different strains of L. monocytogenes were tested, in order to determine the effect of the strain provenience. As determined by cluster analysis, each strain behaved in a different way when inoculated in food juices. The goal was to respond to acidic and osmotic stresses encountered in the food, particularly in the fermented sausage juice. No differences in the expression profile between the three strains were observed, when they were inoculated in BHI. On the other hand, in the meat and sausage juices, the iap, gadC and gadE genes, together with different internalin encoding genes, were significantly differentially expressed in the three strains.
authors.affiliation: Dipartimento di Valorizzazione e Protezione delle Risorse Agroforestali, Section of Agricultural Microbiology and Food Technology, Facoltà di Agraria, Università di Torino, Italy. kalliopi.rantsiou@unito.it
authors.forename: Kalliopi
authors.initials: K
authors.lastname: Rantsiou
authors.forename: Anna
authors.initials: A
authors.lastname: Greppi
authors.forename: Matteo
authors.initials: M
authors.lastname: Garosi
authors.forename: Alberto
authors.initials: A
authors.lastname: Acquadro
authors.forename: Marios
authors.initials: M
authors.lastname: Mataragas
authors.forename: Luca
authors.initials: L
authors.lastname: Cocolin
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D008460:Meat / Q000382:microbiology*; D008461:Meat Products / Q000382:microbiology*; D020411:Oligonucleotide Array Sequence Analysis; D013312:Stress, Physiological; D037521:Virulence Factors / Q000235:genetics
chemical_list: D037521:Virulence Factors