title: Bioactive β-indoloquinazoline alkaloids from Oricia renieri.
abstract: Three new β-indoloquinazoline alkaloids, orirenierine A (1), B (2) and C (4), together with eleven known compounds were isolated from the methanol extract of the stems of Oricia renieri. The structures of all compounds were determined by comprehensive analyses of their spectroscopic data and comparison with literature information. The alkaloids 9-11 were isolated for the first time from this genus. All compounds were tested for their activity against bacteria, fungi, and plant pathogen oomycetes using the paper disk agar diffusion assay. The agar diffusion test gave only low antimicrobial activities, corresponding to MICs > 1 mg/mL. However, compounds 1-4 and 11 exhibited a strong suppressive effect on phagocytosis response upon activation with serum opsonized zymosan in the range of IC(50) = 2.6-6.5 µM, while low cytotoxic activity against the human Caucasian prostate adenocarcinoma cell line PC-3 was observed with IC(50) values ranging from 22.9 to 39.4 µM.
authors.affiliation: Department of Chemistry, University of Douala, Faculty of Science, Douala, Cameroon. jdwansi@yahoo.fr
authors.forename: Jean Duplex
authors.initials: JD
authors.lastname: Wansi
authors.forename: Emmanuel Ngeufa
authors.initials: EN
authors.lastname: Happi
authors.forename: Judith Liliane Djouaka
authors.initials: JL
authors.lastname: Bavoua
authors.forename: Krishna Prasad
authors.initials: KP
authors.lastname: Devkota
authors.forename: Norbert
authors.initials: N
authors.lastname: Sewald
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008297:Male; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009868:Oomycetes / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018547:Plant Stems; D011471:Prostatic Neoplasms / Q000188:drug therapy; D027821:Rutaceae / Q000737:chemistry*; D015054:Zymosan / Q000276:immunology
chemical_list: D000470:Alkaloids; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D015054:Zymosan