title: Silica-coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation.
abstract: Magnetic La(0.75)Sr(0.25)MnO(3) nanoparticles possessing an approximately 20-nm-thick silica shell (LSMO(0.25)@SiO(2) ) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16% PEG 6000-2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.
authors.affiliation: Brno University of Technology, Faculty of Chemistry, Brno, Czech Republic.
authors.forename: Stěpánka
authors.initials: S
authors.lastname: Trachtová
authors.forename: Ondřej
authors.initials: O
authors.lastname: Kaman
authors.forename: Alena
authors.initials: A
authors.lastname: Spanová
authors.forename: Pavel
authors.initials: P
authors.lastname: Veverka
authors.forename: Emil
authors.initials: E
authors.lastname: Pollert
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rittich
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D007778:Lactobacillus / Q000166:cytology / Q000235:genetics; D007811:Lanthanum / Q000737:chemistry*; D060328:Magnetic Phenomena; D008280:Magnetics; D017895:Manganese Compounds / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*; D013324:Strontium / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D007811:Lanthanum; D012822:Silicon Dioxide; D013324:Strontium