title: Loss of Bad expression confers poor prognosis in non-small cell lung cancer.
abstract: Proapoptotic BH-3-only protein Bad (Bcl-Xl/Bcl-2-associated death promoter homolog, Bad) initiates apoptosis in human cells, and contributes to tumorigenesis and chemotherapy resistant in malignancies. This study explored association between the Bad expression level and prognosis in patients with non-small cell lung cancer (NSCLC). In our study, a cohort of 88 resected primary NSCLC cases were collected and analyzed. Bad expression level was determined via immunohistochemical staining assay. The prognostic significances of Bad expression were evaluated with univariate and multivariate survival analysis. The results showed that compared with normal lung tissues, Bad expression level significantly decreased in NSCLC (P < 0.05). Bad expression was associated with adjuvant therapy status. Loss of Bad independently predicted poor prognosis in whole NSCLC cohort and early stage subjects (T1 + T2 and N0 + N1) (all P < 0.05). Overall survival time was also drastically shortened for Bad negative phenotype in NSCLC patients with smoking history, especially lung squamous cell carcinoma (all P < 0.05). In conclusion, this study provided clinical evidence that loss of Bad is an independent and powerful predictor of adverse prognosis in NSCLC. Bad protein could be a new biomarker for selecting individual therapy strategies and predicting therapeutic response in subjects with NSCLC.
authors.affiliation: Laboratory of Genetic Disease and Perinatal Medicine, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Bojiang
authors.initials: B
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Zeng
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Shangfu
authors.initials: S
authors.lastname: Zhang
authors.forename: Xianming
authors.initials: X
authors.lastname: Mo
authors.forename: Weimin
authors.initials: W
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D051025:bcl-Associated Death Protein / Q000032:analysis / Q000096:biosynthesis*
chemical_list: C494819:BAD protein, human; D014408:Biomarkers, Tumor; D051025:bcl-Associated Death Protein