title: Catch and release of alkyne-tagged molecules in water by a polymer-supported cobalt complex.
abstract: A cobalt-phosphine complex supported on PS-PEG beads was found to react with a propargyl carbamate tag, and the tagged molecules immobilized on the beads could be released by acidic treatment through the Nicholas reaction pathway. These reactions work in aqueous media at 4 °C, so that this catch and release procedure is compatible with conditions generally used in biochemical experiments.
authors.affiliation: Sodeoka Live Cell Chemistry Project, ERATO, Japan Science and Technology, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Egami
authors.forename: Shinji
authors.initials: S
authors.lastname: Kamisuki
authors.forename: Kosuke
authors.initials: K
authors.lastname: Dodo
authors.forename: Miwako
authors.initials: M
authors.lastname: Asanuma
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Hamashima
authors.forename: Mikiko
authors.initials: M
authors.lastname: Sodeoka
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002625:Chemistry, Organic / Q000379:methods*; D057930:Click Chemistry / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water
chemical_list: D000480:Alkynes; D010720:Phosphines; D011137:Polystyrenes; C103390:polyethylene glycol-polystyrene; D014867:Water; D001360:Avidin; D003035:Cobalt; D011092:Polyethylene Glycols; D001710:Biotin; D003300:Copper