title: Molecular detection of Anaplasma phagocytophilum in Ixodid ticks in Hebei Province, China.
abstract: A total of 3696 Ixodid ticks, collected from Hebei Province, China, were examined by a nested polymerase chain reaction for the presence of Anaplasma phagocytophilum. Forty-three (15.4%) of 280 pools tested, including 39 (14.6%) of 267 Haemaphysalis longicornis and four (30.8%) of 13 Dermacentor nuttalli, were positive, but no significant difference was found between D. nuttalli and H. longicornis (p>0.05). Sequence and phylogenetic analyses of 16S rRNA gene indicated that A. phagocytophilum in China is genetically diverse. To our knowledge, this is the first evidence of A. phagocytophilum in ticks from Hebei Province, China, and the first documentation of Anaplasma infection in D. nuttalli.
authors.affiliation: Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science and Technology, 360 Hebei Street, Qinhuangdao, China.
authors.forename: Zou
authors.initials: Z
authors.lastname: Yaxue
authors.forename: Jin
authors.initials: J
authors.lastname: Hongtao
authors.forename: Wang
authors.initials: W
authors.lastname: Qiuyue
authors.forename: Fu
authors.initials: F
authors.lastname: Zhixin
authors.forename: Gao
authors.initials: G
authors.lastname: Hongwei
authors.forename: Liu
authors.initials: L
authors.lastname: Pengpeng
authors.forename: Liu
authors.initials: L
authors.lastname: Quan
authors.forename: Chen
authors.initials: C
authors.lastname: Lifeng
mesh_terms: D041081:Anaplasma phagocytophilum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003870:Dermacentor / Q000382:microbiology; D016873:Ehrlichiosis / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D006239:Haplotypes; D006364:Hedgehogs / Q000469:parasitology; D006801:Humans; D026863:Ixodidae / Q000145:classification / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000469:parasitology / Q000635:transmission; D013984:Tick Infestations / Q000469:parasitology / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S