title: Performance evaluation and optimization of multiplex PCRs for the highly discriminating OSU 10-locus set Y-STRs.
abstract: In a previous study, a new set of Y-chromosome short tandem repeats, the OSU 10-locus set (MPM1 and MPM2), was shown to have a higher discrimination power when evaluated against the 10 SWGDAM loci on a common population panel. Here, we describe the optimization of the multiplex reactions using dye-labeled primers followed by performance evaluations. The loci exhibited high precision, human male specificity, reliability in different body fluids, high sensitivity, stability, and the ability to amplify nonprobative casework and mixture samples. Stutter for the all of the loci, with the exception of the highly polymorphic locus DYS688, was similar to that observed for autosomal loci. The results of the performance evaluations reinforced the utility of these loci.
authors.affiliation: Graduate Program in Biomolecular Science, University of Central Florida, Orlando, FL 32816-2366, USA.
authors.forename: Erin
authors.initials: E
authors.lastname: Hanson
authors.forename: Julie L
authors.initials: JL
authors.lastname: Maybruck
authors.forename: Jack
authors.initials: J
authors.lastname: Ballantyne
authors.forename: Paul A
authors.initials: PA
authors.lastname: Fuerst
mesh_terms: D000818:Animals; D001769:Blood; D041322:Chromosomes, Human, Y; D004247:DNA / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004586:Electrophoresis; D005260:Female; D005456:Fluorescent Dyes; D056426:Genetic Loci; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction; D012463:Saliva; D012661:Semen
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D004247:DNA