title: Effect of ultrasound irradiation on α-SMA and TGF-β1 expression in human dermal fibroblasts.
abstract: Ultrasound therapy is used to promote pressure ulcer healing as an adjunctive therapy. However, the efficacy and the scientific basis of this treatment are unclear. We investigated the effect of ultrasound irradiation on alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta1 (TGF-β1) expression in human dermal fibroblasts. These are important factors for acceleration of wound closure. We used pulsed ultrasound of 0, 0.1, 0.5, and 1.0 W/cm2. TGF-β1 and α-SMA mRNA was measured by quantitative real-time polymerase chain reaction, α-SMA protein was examined by western blot, and localization of α-SMA was evaluated by immunofluorescence staining. Expression of α-SMA and TGF-β1 mRNA was increased at 24 h but not at 48 h after ultrasound irradiation. There were significant differences between controls of 0 W/cm² and 0.1 W/cm² with a 1.34 ± 0.26 fold increase in α-SMA (P < 0.05) and a 1.78 ± 0.57 fold increase in TGF-β1 (P < 0.05). Protein levels of α-SMA were also increased and detected in ultrasound irradiated fibroblasts at 24 h. Ultrasound irradiation promotes α-SMA expression in human dermal fibroblasts and this suggests the biological mechanism of ultrasound efficacy on chronic wound treatment.
authors.affiliation: Division of Nutrition and Metabolism, Department of Biophysics, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka, Suma-ku, Kobe 654-0142, Japan. pot_maeshige1026@yahoo.co.jp
authors.forename: Noriaki
authors.initials: N
authors.lastname: Maeshige
authors.forename: Hiroto
authors.initials: H
authors.lastname: Terashi
authors.forename: Michiko
authors.initials: M
authors.lastname: Aoyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Torii
authors.forename: Masaharu
authors.initials: M
authors.lastname: Sugimoto
authors.forename: Makoto
authors.initials: M
authors.lastname: Usami
mesh_terms: D000199:Actins / Q000378:metabolism*; D002460:Cell Line; D003470:Culture Media / Q000032:analysis; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D013016:Sound; D053773:Transforming Growth Factor beta1 / Q000032:analysis / Q000378:metabolism*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D003470:Culture Media; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1