title: The effects of grape seeds polyphenols on SKH-1 mice skin irradiated with multiple doses of UV-B.
abstract: The study investigated the protective activity of red grape seeds (Vitis vinifera L, Burgund Mare variety) (BM) extracts in vivo on multiple doses of ultraviolet radiation (UV)-B-induced deleterious effects in SKH-1 mice skin. Eighty 8-weeks-old female SKH-1 mice were divided into 8 groups: control, vehicle, UV-B irradiated, vehicle+UV-B irradiated, BM 2.5mg polyphenols (PF)/cm(2)+UV-B irradiated, BM 4 mg PF/cm(2)+UV-B irradiated, UV-B+BM 2.5mg PF/cm(2), UV-B+BM 4 mg PF/cm(2). The extract was applied topically before or after each UV-B exposure (240 mJ/cm(2)), for 10 days consecutively. The antioxidant activity of BM extract is higher than gallic acid (k(BM)=0.017, k(gallic acid)=0.013). Multiple doses of UV-B generated the formation of cyclobutane pyrimidine dimers (CPDs) and sunburn cells, increased glutathione peroxidase (GPx) and catalase (CAT) activities respectively glutathione (GSH) and IL-1β levels in skin. In group treated with 2.5mg PF/cm(2) before UV-B irradiation BM extract inhibited UV-B-induced sunburn cells, restored the superoxide dismutase (MnSOD) activity, increased insignificantly CAT and GPx activities and reduced IL-1β level. The BM 4.0 mg PF/cm(2) treatment decreased GSH level and reduced the percentage of CPDs positive cells in skin. Both doses of BM extract administered after UV-B irradiation increased the MnSOD and GPx activities and reduced the formation of sunburn cells in skin. Our results suggest that BM extract might be a potential chemo-preventive candidate in reducing the oxidative stress and apoptosis induced by multiple doses of UV-B in skin.
authors.affiliation: Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 1-3 Clinicilor Str., 400012 Cluj-Napoca, Romania. adrianafilip33@yahoo.com
authors.forename: Adriana
authors.initials: A
authors.lastname: Filip
authors.forename: Doina
authors.initials: D
authors.lastname: Daicoviciu
authors.forename: Simona
authors.initials: S
authors.lastname: Clichici
authors.forename: Pompei
authors.initials: P
authors.lastname: Bolfa
authors.forename: Cornel
authors.initials: C
authors.lastname: Catoi
authors.forename: Ioana
authors.initials: I
authors.lastname: Baldea
authors.forename: Laura
authors.initials: L
authors.lastname: Bolojan
authors.forename: Diana
authors.initials: D
authors.lastname: Olteanu
authors.forename: Adriana
authors.initials: A
authors.lastname: Muresan
authors.forename: I D
authors.initials: ID
authors.lastname: Postescu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols / Q000494:pharmacology*; D011740:Pyrimidine Dimers / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D013471:Sunburn / Q000473:pathology / Q000517:prevention & control; D014466:Ultraviolet Rays / Q000009:adverse effects*; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D010936:Plant Extracts; D059808:Polyphenols; D011740:Pyrimidine Dimers; D008315:Malondialdehyde; D005978:Glutathione