title: Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth.
abstract: BACKGROUND
  Chemokines and cytokines may occur in dentinal fluids in response to local infection and inflammation. To test this hypothesis, we assessed the presence and concentration of inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth.
  
  
  METHODS
  Freshly extracted sound, carious, and restored molars were trimmed through the enamel to expose the underlying dentine, etched with 35% phosphoric acid, and rinsed. Fluid was extracted from the coronal occlusal dentine of these trimmed teeth by centrifugation at 2750 × g for 30 min.
  
  
  RESULTS
  When assessed by MALDI-TOF, fluid extracted from the coronal occlusal dentine from 16 molars contained at least 117 peaks with different masses suggesting that this fluid was rich with molecules within the appropriate mass range of potential mediators. Indeed, when assessed for chemokines and cytokines, fluid extracted from the coronal occlusal dentine from 25 extracted molars with caries lesions, 10 extracted restored molars with occlusal amalgam, and 77 extracted sound molars contained IL-1β, TNF-α, IL-6, IL-8, IL-12(p70), and IL-10. A significant elevation was found for TNF-α (p=0.041) in extracted fluid from teeth restored with amalgam fillings.
  
  
  CONCLUSIONS
  Overall, fluid extracted from the coronal occlusal dentine of trimmed teeth may be useful in identifying proteins and other molecules in dentine and pulpal fluids and determining their role as mediators in the pathogenesis of oral infection and inflammation.
authors.affiliation: Restorative Dental Sciences Operative Division, College of Dentistry, University of Florida, Gainesville, 32610, USA. sgeraldeli@dental.ufl.edu
authors.forename: Saulo
authors.initials: S
authors.lastname: Geraldeli
authors.forename: Yalan
authors.initials: Y
authors.lastname: Li
authors.forename: Mary M B
authors.initials: MM
authors.lastname: Hogan
authors.forename: Leo S
authors.initials: LS
authors.lastname: Tjaderhane
authors.forename: David H
authors.initials: DH
authors.lastname: Pashley
authors.forename: Teresa A
authors.initials: TA
authors.lastname: Morgan
authors.forename: M Bridget
authors.initials: MB
authors.lastname: Zimmerman
authors.forename: Kim A
authors.initials: KA
authors.lastname: Brogden
mesh_terms: D000704:Analysis of Variance; D003731:Dental Caries / Q000473:pathology*; D003804:Dentin / Q000473:pathology*; D019234:Dentinal Fluid / Q000737:chemistry*; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis*; D008297:Male; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018836:Inflammation Mediators