title: Hemorrhagic activity of HF3, a snake venom metalloproteinase: insights from the proteomic analysis of mouse skin and blood plasma.
abstract: Hemorrhage induced by snake venom metalloproteinases (SVMPs) is a complex phenomenon resulting in capillary disruption and blood extravasation. The mechanism of action of SVMPs has been investigated using various methodologies however the precise molecular events associated with microvessel disruption remains not fully understood. To gain insight into the hemorrhagic process, we analyzed the global effects of HF3, an extremely hemorrhagic SVMP from Bothrops jararaca, in the mouse skin and plasma. We report that in the HF3-treated skin there was evidence of degradation of extracellular matrix (collagens and proteoglycans), cytosolic, cytoskeleton, and plasma proteins. Furthermore, the data suggest that direct and indirect effects promoted by HF3 contributed to tissue injury as the activation of collagenases was detected in the HF3-treated skin. In the plasma analysis after depletion of the 20 most abundant proteins, fibronectin appeared as degraded by HF3. In contrast, some plasma proteinase inhibitors showed higher abundance compared to control skin and plasma. This is the first study to assess the complex in vivo effects of HF3 using high-throughput proteomic approaches, and the results underscore a scenario characterized by the interplay between the hydrolysis of intracellular, extracellular, and plasma proteins and the increase of plasma inhibitors in the hemorrhagic process.
authors.affiliation: Laboratório Especial de Toxinologia Aplicada-CAT/cepid, Instituto Butantan, São Paulo, Brazil.
authors.forename: Adriana F
authors.initials: AF
authors.lastname: Paes Leme
authors.forename: Nicholas E
authors.initials: NE
authors.lastname: Sherman
authors.forename: David M
authors.initials: DM
authors.lastname: Smalley
authors.forename: Letícia O
authors.initials: LO
authors.lastname: Sizukusa
authors.forename: Ana K
authors.initials: AK
authors.lastname: Oliveira
authors.forename: Milene C
authors.initials: MC
authors.lastname: Menezes
authors.forename: Jay W
authors.initials: JW
authors.lastname: Fox
authors.forename: Solange M T
authors.initials: SM
authors.lastname: Serrano
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D017837:Bothrops; D003435:Crotalid Venoms / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D006470:Hemorrhage / Q000097:blood* / Q000139:chemically induced; D008297:Male; D045726:Metalloproteases / Q000633:toxicity*; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D053719:Tandem Mass Spectrometry
chemical_list: D001798:Blood Proteins; D003435:Crotalid Venoms; D010446:Peptide Fragments; D020543:Proteome; D045726:Metalloproteases; C543527:HF3 protein, Bothrops jararaca