title: Effects on the conformation of FVIIa by sTF and Ca²⁺ binding: studies of fluorescence resonance energy transfer and quenching.
abstract: The apparent length of FVIIa in solution was estimated by a FRET analysis. Two fluorescent probes, fluorescein (Fl-FPR) and a rhodamine derivative (TMR), were covalently attached to FVIIa. The binding site of Fl-FPR was in the protease domain whereas TMR was positioned in the Gla domain, thus allowing a length measure over virtually the whole extension of the protein. From the FRET measurements, the distances between the two probes were determined to be 61.4 for free FVIIa and 65.5Å for FVIIa bound to soluble tissue factor (sTF). These seemingly short distances, compared to those anticipated based on the complex crystal structure, require that the probes stretch towards each other. Thus, the apparent distance from the FRET analysis was shown to increase with 4Å upon formation of a complex with sTF in solution. However, considering how protein dynamics, based on recent molecular dynamics simulations of FVIIa and sTF:FVIIa (Y.Z. Ohkubo, J.H. Morrissey, E. Tajkhorshid, J. Thromb. Haemost. 8 (2010) 1044-1053), can influence the apparent fluorescence signal our calculations indicated that the global average conformation of active-site inhibited FVIIa is nearly unaltered upon ligation to sTF. It is known from amidolytic activity measurements that Ca(2+) binding leads to activation of FVIIa, but we have for the first time directly demonstrated conformational changes in the environment of the active site upon Ca(2+) binding. Interestingly, this Ca(2+)-induced conformational change can be noted even in the presence of an inhibitor. Forming a complex with sTF further stabilized this conformational change, leading to a more inaccessible active-site located probe.
authors.affiliation: IFM-Department of Chemistry, Linköping University, Linköping, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Carlsson
authors.forename: Egon
authors.initials: E
authors.lastname: Persson
authors.forename: Mikael
authors.initials: M
authors.lastname: Lindgren
authors.forename: Uno
authors.initials: U
authors.lastname: Carlsson
authors.forename: Magdalena
authors.initials: M
authors.lastname: Svensson
mesh_terms: D019943:Amino Acid Substitution; D002118:Calcium / Q000737:chemistry*; D020134:Catalytic Domain; D004789:Enzyme Activation; D015942:Factor VIIa / Q000737:chemistry* / Q000235:genetics; D031541:Fluorescence Resonance Energy Transfer; D011485:Protein Binding; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D013925:Thromboplastin / Q000737:chemistry*
chemical_list: D013925:Thromboplastin; D015942:Factor VIIa; D002118:Calcium