title: Cardio-protection of salvianolic acid B through inhibition of apoptosis network.
abstract: Targeting cellular function as a system rather than on the level of the single target significantly increases therapeutic potency. In the present study, we detect the target pathway of salvianolic acid B (SalB) in vivo. Acute myocardial infarction (AMI) was induced in rats followed by the treatment with 10 mg/kg SalB. Hemodynamic detection and pathological stain, 2-dimensional electrophoresis, MALDI-TOF MS/MS, Western blot, pathway identification, apoptosis assay and transmission electron microscope were used to elucidate the effects and mechanism of SalB on cardioprotection. Higher SalB concentration was found in ischemic area compared to no-ischemic area of heart, correlating with improved heart function and histological structure. Thirty-three proteins regulated by SalB in AMI rats were identified by biochemical analysis and were classified as the components of metabolism and apoptosis networks. SalB protected cardiomyocytes from apoptosis, inhibited poly (ADP-ribose) polymerase-1 pathway, and improved the integrity of mitochondrial and nucleus of heart tissue during AMI. Furthermore, the protective effects of SalB against apoptosis were verified in H9c2 cells. Our results provide evidence that SalB regulates multi-targets involved in the apoptosis pathway during AMI and therefore may be a candidate for novel therapeutics of heart diseases.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Deng
authors.forename: Lixin
authors.initials: L
authors.lastname: Feng
authors.forename: Defang
authors.initials: D
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Fukang
authors.initials: F
authors.lastname: Teng
authors.forename: Wanying
authors.initials: W
authors.lastname: Wu
authors.forename: Shuhong
authors.initials: S
authors.lastname: Guan
authors.forename: Baohong
authors.initials: B
authors.lastname: Jiang
authors.forename: Dean
authors.initials: D
authors.lastname: Guo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001572:Benzofurans / Q000627:therapeutic use*; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006439:Hemodynamics / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D008297:Male; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D053630:Depsides
chemical_list: D001572:Benzofurans; D016328:NF-kappa B; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; C076944:salvianolic acid B; D053630:Depsides; C499322:Parp1 protein, rat