title: Biomarkers for visceral hypersensitivity identified by classification of electroencephalographic frequency alterations.
abstract: Abdominal pain is frequently related to visceral hypersensitivity. This is associated with increased neuronal excitability in the central nervous system (CNS), which can be manifested as discrete electroencephalographic (EEG) alterations. In the current placebo-controlled study, visceral hypersensitivity was evoked by chemical irritation of the esophagus with acid and capsaicin perfusion. The resulting hyperexcitability of the CNS was evaluated by evoked brain potentials following painful electrical stimulations of a remote organ--the rectosigmoid colon. Alterations in individual EEG power distributions between baseline and after perfusion were quantified by extracting features from the evoked brain potentials using an optimized discrete wavelet transform. Visceral hypersensitivity was identified as increased EEG power in the delta, theta and alpha frequency bands. By applying a support vector machine in regression mode, the individual baseline corrected alterations after sensitization were discriminated from alterations caused by placebo perfusions. An accuracy of 91.7% was obtained (P < 0.01). The regression value representing the overall alteration of the EEG correlated with the degree of hyperalgesia (P = 0.03). In conclusion, this study showed that classification of EEG can be used to detect biomarkers reflecting central neuronal changes. In the future, this may be used in studies of pain physiology and pharmacological interventions.
authors.affiliation: Mech-Sense, Department of Gastroenterology, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark.
authors.forename: Carina
authors.initials: C
authors.lastname: Graversen
authors.forename: Christina
authors.initials: C
authors.lastname: Brock
authors.forename: Asbjørn Mohr
authors.initials: AM
authors.lastname: Drewes
authors.forename: Dario
authors.initials: D
authors.lastname: Farina
mesh_terms: D000143:Acids; D000328:Adult; D015415:Biomarkers; D002211:Capsaicin; D012809:Colon, Sigmoid / Q000502:physiology; D018592:Cross-Over Studies; D003627:Data Interpretation, Statistical; D004558:Electric Stimulation; D004569:Electroencephalography / Q000145:classification* / Q000379:methods*; D004947:Esophagus / Q000502:physiology; D005260:Female; D006801:Humans; D006930:Hyperalgesia / Q000139:chemically induced / Q000503:physiopathology*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D058748:Pain Perception / Q000502:physiology; D017288:Pain Threshold / Q000502:physiology; D012044:Regression Analysis; D015203:Reproducibility of Results; D014584:User-Computer Interface; D058067:Wavelet Analysis; D055815:Young Adult
chemical_list: D000143:Acids; D015415:Biomarkers; D002211:Capsaicin