title: Charcoal filter paper improves the viability of cryopreserved filamentous ectomycorrhizal and saprotrophic Basidiomycota and Ascomycota.
abstract: We assessed viability of 18 strains of filamentous ectomycorrhizal and saprotrophic basid-iomycetes and ascomycetes after cryopreservation with a novel technique based on charcoal filter paper strips (CFS). The results indicate that axenic fungal cultures grown on CFS recovered from freezing within a few days, even though none survived cryopreservation by the conventional straw method. Fungal growth on CFS was more vigorous, with morphological differentiations such as rhizomorphs and an increased amount of aerial mycelia compared to the unamended culture media. Accordingly CFS allows the cryopreservation of a wide range of rare and important ectomycorrhizal and saprotrophic fungi, which hitherto were difficult to revive from liquid nitrogen storage with the conventional and widely applied straw technique.
authors.affiliation: German Collection for Microorganisms and Cell Cultures GmbH, Braunschweig, Germany.
authors.forename: J Benjamin
authors.initials: JB
authors.lastname: Stielow
authors.forename: Lea A I
authors.initials: LA
authors.lastname: Vaas
authors.forename: Markus
authors.initials: M
authors.lastname: Göker
authors.forename: Peter
authors.initials: P
authors.lastname: Hoffmann
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D001203:Ascomycota / Q000254:growth & development*; D061245:Axenic Culture / Q000379:methods; D001487:Basidiomycota / Q000254:growth & development*; D002606:Charcoal / Q000737:chemistry*; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D050296:Microbial Viability; D025282:Mycelium / Q000254:growth & development; D038821:Mycorrhizae / Q000254:growth & development; D010209:Paper
chemical_list: D003470:Culture Media; D002606:Charcoal