title: RapidChek SELECT Salmonella enteritidis test system for the detection of Salmonella enteritidis in poultry house drag swabs, shell egg pools, and chicken carcass rinsates.
abstract: The RapidChek SELECT Salmonella Enteritidis Test System was validated for the detection of Salmonella Enteritidis (SE) in poultry house drag swabs, shell egg pools, and chicken carcass rinsates. The method utilizes RapidChek SELECT Salmonella (AOAC PTM License No. 080601) proprietary primary and secondary enrichment media. Following enrichment, an immunochromatographic test strip is inserted into the tube containing the secondary enrichment broth, developed for 10 min, and interpreted. Salmonella Enteritidis-inoculated samples (1-5 CFU SE/analytical unit) were tested by the test method as well as the appropriate cultural reference method U.S. Food and Drug Administration-Bacteriological Analytical Manual (drag swabs and egg pools) or U.S. Department of Agriculture-Food Safety and Inspection Service (chicken carcass rinsates). A total of 80 samples were tested by both methods in the study. Fifty-two samples were positive by the RapidChek SELECT Salmonella Enteritidis method and 38 were found positive by the respective reference method. The sensitivity of the method was 100% and the specificity was 100%. The accuracy of the test method was 137%, indicating that the method was more sensitive than the reference method. The RapidChek SELECT Salmonella Enteritidis method was tested with 82 Salmonella Group D1 strains including 63 Salmonella Enteritidis strains as well as 32 non-Salmonella Group D1 strains representing 10 bacteria genera. The test method detected all 82 Group D1 strains (100% sensitivity). None of the non-Salmonella Group D1 or other genera of bacteria were detected, indicating a specificity of 100%. The method was shown to be highly robust and stable under control and accelerated stability conditions.
authors.affiliation: SDIX, 128 Sandy Dr, Newark, DE 19713, USA. mmuldoon@sdix.com
authors.forename: Mark T
authors.initials: MT
authors.lastname: Muldoon
authors.forename: Verapaz
authors.initials: V
authors.lastname: Gonzalez
authors.forename: Meredith I
authors.initials: MI
authors.lastname: Sutzko
authors.forename: Ann-Christine Olsson
authors.initials: AC
authors.lastname: Allen
authors.forename: Samantha
authors.initials: S
authors.lastname: Creamer
authors.forename: Dale V
authors.initials: DV
authors.lastname: Onisk
authors.forename: Klaus
authors.initials: K
authors.lastname: Lindpaintner
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media; D004531:Eggs / Q000382:microbiology*; D006799:Housing, Animal; D007202:Indicators and Reagents; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic