title: No detectable XMRV in subjects with chronic fatigue syndrome from Quebec.
abstract: We investigated the presence of XMRV in a cohort of Quebec patients with chronic fatigue syndrome (CFS). DNA was purified from activated peripheral blood mononuclear cells (PBMCs) and PCR was used to detect XMRV gag and env in 72 patients. Anti-XMRV antibodies were searched in sera of 62 patients by Western blot analysis. Attempts to detect XMRV antigens was made, using immunofluorescence with Gag anti-p30 antiserum on activated PBMC from 50 patients. Plasma viremia was measured by RT-PCR on 9 subjects. Finally, detection of infectious virus in 113 CFS subjects was made by co-culture of PHA+IL-2 activated PBMC with human LNCaP carcinoma cells, and by infecting the same susceptible cells with plasma, using a reverse transcriptase (RT) assay as a readout in both experiments. No detection of XMRV footprints nor infectious virus was detected with any of the approaches, in any of the tested individuals.
authors.affiliation: Laboratory of Molecular Biology, Clinical Research Institute of Montreal, Montreal, Quebec, Canada H2W 1R7.
authors.forename: Marc
authors.initials: M
authors.lastname: Cool
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bouchard
authors.forename: Ginette
authors.initials: G
authors.lastname: Massé
authors.forename: Benoît
authors.initials: B
authors.lastname: Laganière
authors.forename: Agnès
authors.initials: A
authors.lastname: Dumont
authors.forename: Zaher
authors.initials: Z
authors.lastname: Hanna
authors.forename: Denis
authors.initials: D
authors.lastname: Phaneuf
authors.forename: Richard
authors.initials: R
authors.lastname: Morisset
authors.forename: Paul
authors.initials: P
authors.lastname: Jolicoeur
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics; D015673:Fatigue Syndrome, Chronic / Q000175:diagnosis / Q000821:virology*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D011792:Quebec; D058008:Xenotropic murine leukemia virus-related virus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral