title: A genetic in vivo system to detect asymmetrically distributed RNA.
abstract: Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.
authors.affiliation: Institute of Genetics, University of Cologne, Zülpicher Strasse 47, Cologne 50674, Germany.
authors.forename: N
authors.initials: N
authors.lastname: JayaNandanan
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Gavis
authors.forename: Veit
authors.initials: V
authors.lastname: Riechmann
authors.forename: Maria
authors.initials: M
authors.lastname: Leptin
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D004331:Drosophila melanogaster / Q000235:genetics*; D005820:Genetic Testing; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D017909:Levivirus / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D010865:Pilot Projects; D014176:Protein Biosynthesis / Q000235:genetics; D012313:RNA / Q000378:metabolism*; D034443:RNA Transport / Q000235:genetics*; D015203:Reproducibility of Results
chemical_list: D049452:Green Fluorescent Proteins; D012313:RNA