title: Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats.
abstract: The aim of the present study was to examine the effect of sympathectomy on plasmatic and arterial native and oxLDL levels, as well as arterial LDL receptors (LDLR) and scavenger receptors in hypercholesterolemic rats, which are normally protected against atherosclerosis. Neonatal Wistar rats received subcutaneous injections of either guanethidine for sympathectomy (Gua+HC) or vehicle (HC), then were fed 1% cholesterol for three months. Intact normocholesterolemic rats were used as control of the HC group. Total cholesterol (TC) and LDL-cholesterol were evaluated in the plasma and the abdominal aorta by an auto-analyzer. Plasmatic and aortic oxLDL and native LDL-apo B100 were assessed by a sandwich ELISA. Aortic and hepatic native LDLR and aortic scavenger receptors (CD36 and SR-A) were quantified at mRNA and protein levels by real time PCR and western immunoblot. The effect of hypercholesterolemia was limited to an increase in plasmatic TC and LDL-cholesterol and a decrease in aortic apoB100 and aortic and hepatic LDLR. Hypercholesterolemia and sympathectomy in combination increased markedly plasmatic and aortic TC, LDL-cholesterol, apo B100 and oxLDL together with aortic scavenger receptors, but reduced markedly aortic and hepatic LDLR. Sympathectomy broke down the rat's protection against hypercholesterolemia by promoting accumulation of native and oxLDL in the aorta via scavenger receptors.
authors.affiliation: Université de Carthage, Unité de Physiologie Intégré, Laboratoire de Pathologies Vasculaires, Faculté des Sciences de Bizerte, 7021 Jarzouna, Tunisia. rafik.hachani@fsb.rnu.tn
authors.forename: Rafik
authors.initials: R
authors.lastname: Hachani
authors.forename: Houcine
authors.initials: H
authors.lastname: Dab
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.forename: Eric
authors.initials: E
authors.lastname: Vicaut
authors.forename: Jacques
authors.initials: J
authors.lastname: Callebert
authors.forename: Richard
authors.initials: R
authors.lastname: Sercombe
authors.forename: Kamel
authors.initials: K
authors.lastname: Kacem
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001158:Arteries / Q000378:metabolism* / Q000503:physiopathology; D001342:Autonomic Nervous System Diseases / Q000097:blood* / Q000150:complications / Q000503:physiopathology; D002784:Cholesterol / Q000097:blood*; D004195:Disease Models, Animal; D006937:Hypercholesterolemia / Q000097:blood* / Q000209:etiology / Q000503:physiopathology; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D002784:Cholesterol