title: Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced-based PCR fingerprinting.
abstract: BACKGROUND
  Four repetitive element sequence-based polymerase chain reaction (rep-PCR) methods, namely repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus PCR (ERIC-PCR), polytrinucleotide (GTG)₅ -PCR and BOX-PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep-PCR methods and their combination (composite rep-PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D).
  
  
  RESULTS
  Species-specific and strain-specific profiles were observed from rep-PCR. From the numerical analysis of composite rep-PCR, BOX-PCR, (GTG)₅ -PCR, REP-PCR and ERIC-PCR, D values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep-PCR analysis was the most discriminative method, with eight Lactobacillus strains, namely L. brevis ATCC 14869(T) , L. reuteri C 10, L. reuteri ATCC 23272(T) , L. gallinarum ATCC 33199(T) , L. salivarius ATCC 11741(T) , L. salivarius I 24, L. panis JCM 11053(T) and L. panis C 17, being differentiated at the strain level.
  
  
  CONCLUSIONS
  Composite rep-PCR analysis is potentially a useful fingerprinting method to discriminate probiotic Lactobacillus strains isolated from the gastrointestinal tract of chickens.
authors.affiliation: Laboratory of Industrial Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
authors.forename: Chin Mei
authors.initials: CM
authors.lastname: Lee
authors.forename: Chin Chin
authors.initials: CC
authors.lastname: Sieo
authors.forename: Yoke-Kqueen
authors.initials: YK
authors.lastname: Cheah
authors.forename: Norhani
authors.initials: N
authors.lastname: Abdullah
authors.forename: Yin Wan
authors.initials: YW
authors.lastname: Ho
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology; D016172:DNA Fingerprinting / Q000662:veterinary; D004269:DNA, Bacterial / Q000378:metabolism*; D004032:Diet / Q000662:veterinary*; D041981:Gastrointestinal Tract / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology; D055029:Inverted Repeat Sequences; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008296:Malaysia; D058889:Molecular Typing / Q000379:methods / Q000662:veterinary; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019936:Probiotics / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial