title: The intensity of bronchiolar epithelial cell injury caused by an alloimmune response is ameliorated by transbronchial human interleukin-10 gene transfer in a rat model of lung transplantation.
abstract: The aim of the present study was to determine whether the intensity of bronchiolar epithelial cell injury is ameliorated by transbronchial human IL-10 (hIL-10) gene transfer in a rat model of lung allograft rejection. The left lung was extracted from a donor Brown Norway rat and transbronchially instilled with encoding hIL-10 (IL-10 group) or β-galactosidase (control group). The lung graft was then transplanted into a Lewis rat and harvested on day 6 after transplantation. The allografts were histologically examined and all bronchioles in the slides were assigned one of three grades according to the intensity of epithelial cell loss. The distribution of the grades was significantly different between the two groups, and the epithelial cell injury was significantly improved in the IL-10 group. The present study demonstrated the effect of transbronchial hIL-10 gene transfer on ameliorating bronchiolar epithelial cell injury in a rat model of lung allograft rejection.
authors.affiliation: Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai, 980-8575, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Oishi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Okada
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Tetsu
authors.initials: T
authors.lastname: Sado
authors.forename: Masafumi
authors.initials: M
authors.lastname: Noda
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Hoshikawa
authors.forename: Akira
authors.initials: A
authors.lastname: Sakurada
authors.forename: Chiaki
authors.initials: C
authors.lastname: Endo
authors.forename: Takashi
authors.initials: T
authors.lastname: Kondo
mesh_terms: D000818:Animals; D055745:Bronchioles / Q000473:pathology*; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology; D016040:Lung Transplantation / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D020545:Respiratory Mucosa / Q000473:pathology*
chemical_list: D016753:Interleukin-10