title: Free radicals and redox signalling in T-cells during chronic inflammation and ageing.
abstract: During chronic inflammation and ageing, the increase in oxidative stress in both intracellular and extracellular compartments is likely to influence local cell functions. Redox changes alter the T-cell proteome in a quantitative and qualitative manner, and post-translational modifications to surface and cytoplasmic proteins by increased reactive species can influence T-cell function. Previously, we have shown that RA (rheumatoid arthritis) T-cells exhibit reduced ROS (reactive oxygen species) production in response to extracellular stimulation compared with age-matched controls, and basal ROS levels [measured as DCF (2',7'-dichlorofluorescein) fluorescence] are lower in RA T-cells. In contrast, exposing T-cells in vitro to different extracellular redox environments modulates intracellular signalling and enhances cytokine secretion. Together, these data suggest that a complex relationship exists between intra- and extra-cellular redox compartments which contribute to the T-cell phenotype.
authors.affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK. h.r.griffiths@aston.ac.uk
authors.forename: Helen R
authors.initials: HR
authors.lastname: Griffiths
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Dunston
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Bennett
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Grant
authors.forename: Darren C
authors.initials: DC
authors.lastname: Phillips
authors.forename: George D
authors.initials: GD
authors.lastname: Kitas
mesh_terms: D000375:Aging / Q000502:physiology*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology*
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species