title: Prevalence and genetic diversity of norovirus in outpatient children with acute diarrhea in Shanghai, China.
abstract: This study aimed to investigate the epidemiology of norovirus (NoV) associated-diarrhea among pediatric outpatients in Shanghai, and characterize the genotypes of circulating NoV strain. Stool samples were collected from 910 children with non-dysenteric diarrhea between August 2008 and July 2009. One-step real-time RT-PCR was used to screen for NoV genogroup I (GI) and genogroup II (GII). Genotypes were classified by sequence analysis of partial capsid and RNA-dependent RNA polymerase (RdRp) fragments. NoV was detected year round with high activity in July, August, September, and October. Of 910 specimens, 165 (18.13%) were positive for NoV; 4 (2.42%) were GI, and 161 (97.58%) were GII. Based on capsid sequences, 8 different genotypes were identified for 114 NoV strains, including GII.4 2006b (57.89%), GII.3 (30.70%), GII.6 (4.39%), GII.12 (3.51%), GII.14, GII.2, GI.4, and GI.5 (0.88% each). Based on the RdRp sequences of 86 NoV strains, NoV was genotyped as GII.4 (62.79%), GII.12 (30.23%), GII.g (2.33%), GII.2 (1.16%), GII.6 (1.16%), GII.7 (1.16%), and GI.4 (1.16%). The RdRp genotypes of 30 strains were inconsistent with the capsid genotypes, indicating potential NoV recombinants.
authors.affiliation: Department of Infectious Diseases & Research Laboratory of Infectious Diseases, Children's Hospital of Fudan University, Shanghai, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zeng
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Gong
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qirong
authors.initials: Q
authors.lastname: Zhu
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
mesh_terms: D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology*; D036022:Capsid Proteins / Q000235:genetics; D002648:Child; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D029322:Norovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010045:Outpatients; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral