title: Incorporation of the HIV-1 microbicide cyanovirin-N in a food product.
abstract: An urgent need exists for HIV-1 microbicides. Here, we describe the in vivo testing of lactic acid bacteria bioengineered to secrete cyanovirin-N. We fed pigtail macaques a yogurt formulation that used bioengineered strains as a starter culture. Cyanovirin-N expression could be detected in the rectal vault during and immediately after feeding. Ex vivo viral challenge of rectal tissue biopsies revealed that peak viral burden was significantly lower in tissue obtained from experimental animals compared with control animals. Formulation of candidate compounds in lactic acid bacteria and their oral administration seems to be a feasible strategy for mucosal delivery of microbicides.
authors.affiliation: Laboratory of Retrovirology, Division of Infectious Diseases, Department of Medicine, Rhode Island and Miriam Hospitals, Warren Alpert Medical School of Brown University, Providence, RI, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Dorothy L
authors.initials: DL
authors.lastname: Patton
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Cosgrove-Sweeney
authors.forename: Deena
authors.initials: D
authors.lastname: Ratner
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Rohan
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Cole
authors.forename: Patrick M
authors.initials: PM
authors.lastname: Tarwater
authors.forename: Phalguni
authors.initials: P
authors.lastname: Gupta
authors.forename: Bharat
authors.initials: B
authors.lastname: Ramratnam
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000008:administration & dosage; D001426:Bacterial Proteins / Q000008:administration & dosage*; D002352:Carrier Proteins / Q000008:administration & dosage*; D005243:Feces / Q000737:chemistry / Q000821:virology; D005260:Female; D005503:Food Additives / Q000008:administration & dosage*; D015658:HIV Infections / Q000517:prevention & control; D015497:HIV-1 / Q000187:drug effects; D008254:Macaca nemestrina; D012007:Rectum / Q000737:chemistry / Q000187:drug effects / Q000821:virology; D014779:Virus Replication / Q000187:drug effects; D015014:Yogurt
chemical_list: D019380:Anti-HIV Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005503:Food Additives; C107733:cyanovirin N