title: Activity of soluble aminopeptidase A and dipeptidyl peptidase IV and membrane-bound aminopeptidase B and pyroglutamyl peptidase I in adenoid hyperplasia, tonsillar hyperplasia and chronic tonsillitis.
abstract: OBJECTIVE
  To analyze soluble and membrane-bound peptidase activities in the tonsils and adenoids removed from patients with adenoid hyperplasia, tonsillar hyperplasia and chronic tonsillitis.
  
  
  METHODS
  A total of 48 tissue samples from patients undergoing adenoidectomy and tonsillectomy for adenoid hyperplasia, tonsillar hyperplasia or chronic tonsillitis were analyzed. The catalytic activity of a pool of peptidases in the soluble (dipeptidyl peptidase IV, aminopeptidase A, aminopeptidase N and cystinyl aminopeptidase) and membrane-bound (prolyl endopeptidase, aspartyl aminopeptidase, aminopeptidase B and pyroglutamyl peptidase I) fractions was measured fluorometrically.
  
  
  RESULTS
  The activity of membrane-bound aminopeptidase B was higher in cases of chronic tonsillitis and adenoid hyperplasia than in tonsillar hyperplasia, p=0.004. Soluble dipeptidyl peptidase IV and membrane-bound pyroglutamyl peptidase I were found to be more active in tissues from male chronic tonsillitis tissues, p<0.05, while membrane-bound aminopeptidase B activity was higher in tissues of females with tonsillar hyperplasia, p<0.001. In the case of chronic tonsillitis, soluble aminopeptidase A was found to have a higher level of activity in tissues from children than those from adults, p=0.005.
  
  
  CONCLUSIONS
  Our results suggest a potential role of soluble aminopeptidase A, soluble dipeptidyl peptidase IV, membrane-bound aminopeptidase B and membrane-bound pyroglutamyl peptidase I in the pathobiology of adenoid hyperplasia, tonsillar hyperplasia and chronic tonsillitis that is differently regulated as a function of gender. These finfings may modify in the future the clinical approach to these diseases.
authors.affiliation: Department of Physiology, School of Medicine, University of the Basque Country, Spain.
authors.forename: Gorka
authors.initials: G
authors.lastname: Larrinaga
authors.forename: Itxaro
authors.initials: I
authors.lastname: Perez
authors.forename: Begoña
authors.initials: B
authors.lastname: Sanz
authors.forename: Amaya
authors.initials: A
authors.lastname: Irazusta
authors.forename: Idoia
authors.initials: I
authors.lastname: Zarrazquin
authors.forename: Clara Elena
authors.initials: CE
authors.lastname: Sanchez
authors.forename: Carmen Elena
authors.initials: CE
authors.lastname: Sanchez
authors.forename: Ana Sanchez Del
authors.initials: AS
authors.lastname: Rey
authors.forename: Aitor
authors.initials: A
authors.lastname: Zabala
authors.forename: Francisco
authors.initials: F
authors.lastname: Santaolalla
mesh_terms: D000234:Adenoids / Q000378:metabolism* / Q000473:pathology; D000626:Aminopeptidases / Q000032:analysis / Q000378:metabolism*; D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D015331:Cohort Studies; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis / Q000378:metabolism; D005260:Female; D043384:Glutamyl Aminopeptidase / Q000032:analysis / Q000378:metabolism; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism / Q000473:pathology; D008297:Male; D014066:Palatine Tonsil / Q000378:metabolism* / Q000473:pathology; D011751:Pyroglutamyl-Peptidase I / Q000032:analysis / Q000378:metabolism; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D014068:Tonsillectomy / Q000379:methods; D014069:Tonsillitis / Q000378:metabolism / Q000473:pathology / Q000601:surgery
chemical_list: D015415:Biomarkers; D000626:Aminopeptidases; C019721:aminopeptidase B; D043384:Glutamyl Aminopeptidase; D018819:Dipeptidyl Peptidase 4; D011751:Pyroglutamyl-Peptidase I