title: The role of multifunctional delivery scaffold in the ability of cultured myoblasts to promote muscle regeneration.
abstract: Many cell types of therapeutic interest, including myoblasts, exhibit reduced engraftment if cultured prior to transplantation. This study investigated whether polymeric scaffolds that direct cultured myoblasts to migrate outwards and repopulate the host damaged tissue, in concert with release of angiogenic factors designed to enhance revascularizaton of the regenerating tissue, would enhance the efficacy of this cell therapy and lead to functional muscle regeneration. This was investigated in the context of a severe injury to skeletal muscle tissue involving both myotoxin-mediated direct damage and induction of regional ischemia. Local and sustained release of VEGF and IGF-1 from macroporous scaffolds used to transplant and disperse cultured myogenic cells significantly enhanced their engraftment, limited fibrosis, and accelerated the regenerative process. This resulted in increased muscle mass and, improved contractile function. These results demonstrate the importance of finely controlling the microenvironment of transplanted cells in the treatment of severe muscle damage.
authors.affiliation: School of Engineering and Applied Sciences, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Borselli
authors.forename: Christine A
authors.initials: CA
authors.lastname: Cezar
authors.forename: Dymitri
authors.initials: D
authors.lastname: Shvartsman
authors.forename: Herman H
authors.initials: HH
authors.lastname: Vandenburgh
authors.forename: David J
authors.initials: DJ
authors.lastname: Mooney
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000502:physiology*; D032446:Myoblasts / Q000166:cytology*; D012038:Regeneration; D054457:Tissue Scaffolds / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D042461:Vascular Endothelial Growth Factor A; D007334:Insulin-Like Growth Factor I; D020723:Glucuronic Acid