title: Hepsin immunohistochemical expression in prostate cancer in relation to Gleason's grade and serum prostate specific antigen.
abstract: BACKGROUND
  Hepsin (HPN), a type II trans-membrane serine protease, has been reported to be one of the most up regulated genes in prostate cancer. The aim of the present study was to find out immuno-histochemistry based phenotypic expression of HPN in formalin fixed paraffin embedded sections of prostate cancer compared with that in benign prostatic hyperplasia, in a prospective clinical setting, to know the differential status of HPN expression in benign and malignant prostatic disease.
  
  
  METHODS
  Tissue biopsies of histologically proven cases of prostatic cancers (48), benign prostatic hyperplasia (42), benign prostatic hyperplasia with prostatic intraepithelial neoplasia (7) and 4 cases of benign prostatic hyperplasia with prostatitis, were subjected to immunohistochemical staining with HPN antibody by strepavidin biotin method.
  
  
  RESULTS
  Hepsin expression was 100% in prostate carcinoma, 11.9% (5/42) in benign prostatic hyperplasia, 57.14% (4/7) in benign prostatic hyperplasia with prostatic intraepithelial neoplasia (PIN), and none in benign prostatic hyperplasia with prostatitis. Hepsin staining showed higher expression in high grade tumor in comparison to low grade tumor.
  
  
  CONCLUSIONS
  Positive immunohistochemical expression of hepsin in cent percent cases of prostate cancer cases is intriguing, underscoring the significance of hepsin gene expression in prostate cancer.
authors.affiliation: Department of Pathology, CSM Medical University, Lucknow, UP, India. madhukgmc@rediffmail.com
authors.forename: Madhu Mati
authors.initials: MM
authors.lastname: Goel
authors.forename: Dhamita
authors.initials: D
authors.lastname: Agrawal
authors.forename: S M
authors.initials: SM
authors.lastname: Natu
authors.forename: Apul
authors.initials: A
authors.lastname: Goel
mesh_terms: D001706:Biopsy; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008853:Microscopy; D011446:Prospective Studies; D011467:Prostate / Q000473:pathology*; D017430:Prostate-Specific Antigen / Q000097:blood*; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D012697:Serine Endopeptidases / Q000032:analysis*; D044967:Serum / Q000737:chemistry
chemical_list: D012697:Serine Endopeptidases; C055645:hepsin; D017430:Prostate-Specific Antigen