title: [The application value of water flea Daphnia pulex for hypoxia model].
abstract: Hypoxia-inducible factor (HIF) is an important transcription factor under hypoxic condition in many organisms, and plays a key role in the induction of hypoxia tolerance. It is necessary to establish a hypoxia model for HIF and to perform further hypoxia tolerance research. To investigate the value of Daphnia as a model organism in hypoxia precondition, we developed a preconditioning protocol with a model organism, Daphnia pulex. We found that two episodes of exposure to hypoxic solution resulted in enhanced hypoxia tolerance which is dependent on HIF. Comparative genomic analysis was also made to highlight the homology of HIF-related genes among Daphnia, fruitfly and human. We found that Daphnia is suitable for the study of human hypoxic injury as a model organism.
authors.affiliation: Laboratory of Anesthesiology and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Li
authors.forename: Bo
authors.initials: B
authors.lastname: Sheng
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Zuo
authors.forename: Jin
authors.initials: J
authors.lastname: Lin
authors.forename: Guohua
authors.initials: G
authors.lastname: Li
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D033361:Cladocera / Q000235:genetics; D046508:Culture Techniques; D003621:Daphnia / Q000235:genetics*; D004195:Disease Models, Animal; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D051793:Hypoxia-Inducible Factor 1 / Q000235:genetics*; D019194:Ischemic Preconditioning / Q000379:methods; D008969:Molecular Sequence Data
chemical_list: D051793:Hypoxia-Inducible Factor 1