title: Effect of contamination and decontamination on adhesion of a resin-modified glass-ionomer cement to bovine dentin.
abstract: OBJECTIVE
  To determine the adhesion of resin-modified glass-ionomer cement to bovine dentin under contaminated and decontaminated conditions.
  
  
  METHODS
  Forty-five bovine mandibular incisors were used. The surfaces of bovine dentin specimens were subjected to Temp-bond, dental handpiece lubricant (contamination), Hibiscrub, chlorhexidine or pumice (decontamination), as well as contamination followed by decontamination. From these, 14 test groups were created to investigate the effects of these variables on the microtensile bond strength of a resin-modified glassionomer cement to dentin. In addition, scanning electron microscopy was performed to examine the effects of contamination and decontamination procedures on the dentin surfaces. The data were analyzed by one-way ANOVA, Kruskal-Wallis, and Mann-Whitney tests.
  
  
  RESULTS
  SEM examination showed visible differences between the control group and dentin contaminated with Temp-bond or handpiece lubricant. All the contamination and decontamination test agents when used alone - except Hibiscrub - showed significant reductions in bond strength when compared to the control (p < 0.001). All the test groups subjected to contamination followed by decontamination showed a significantly reduced bond strength (p < 0.001) when compared to the control, with the exception of the handpiece lubricant/Hibiscrub combination.
  
  
  CONCLUSIONS
  Under the conditions tested, Temp-bond, handpiece lubricant, chlorhexidine, and pumice may have an adverse effect on the bonding of resin-modified glass ionomer to dentin. Hibiscrub was effective in decontaminating handpiece lubricant but not Temp-bond.
authors.affiliation: Department of Oral Rehabilitation, the University of Hong Kong, Hong Kong.
authors.forename: Pitsapaporn
authors.initials: P
authors.lastname: Wangpermtam
authors.forename: Michael G
authors.initials: MG
authors.lastname: Botelho
authors.forename: John E
authors.initials: JE
authors.lastname: Dyson
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000180:Acrylic Resins / Q000737:chemistry; D000268:Adhesiveness; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000737:chemistry*; D002417:Cattle; D002710:Chlorhexidine / Q000031:analogs & derivatives / Q000737:chemistry; D003666:Decontamination; D001840:Dental Bonding; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D005054:Eugenol / Q000737:chemistry; D005899:Glass Ionomer Cements / Q000737:chemistry*; D006838:Hydrocarbons / Q000737:chemistry; D054327:Lubricants / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D009067:Mouthwashes / Q000737:chemistry; D011897:Random Allocation; D019279:Resin Cements / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D015034:Zinc Oxide / Q000737:chemistry; D015035:Zinc Oxide-Eugenol Cement / Q000737:chemistry
chemical_list: D000180:Acrylic Resins; D000891:Anti-Infective Agents, Local; D005899:Glass Ionomer Cements; D006838:Hydrocarbons; D054327:Lubricants; D009067:Mouthwashes; D019279:Resin Cements; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; D005054:Eugenol; C006903:carbopol 940; C076899:Temp-Bond; C010882:chlorhexidine gluconate; C005144:pumice; D002710:Chlorhexidine; D015034:Zinc Oxide