title: [Near-infrared fluorescent zinc-dipicolylamine: a new molecular imaging probe to monitor the efficiency of chemotherapy].
abstract: OBJECTIVE
  To investigate the feasibility of a novel molecular probe of Zn-DPA-PSS794 to monitor the efficiency of doxorubicin to ovarian cancer and compare with Cy5.5-annexin V.
  
  
  METHODS
  Efficiency of doxorubicin to OVCAR-8 cells in vitro was measured by MTT assay and flow cytometry. The in vivo studies were performed on an OVCAR-8 xenograft tumor model. Mice were divided into a control group and a treatment group. Each group was divided into 2 subgroups, DPA and annexin V. In the treatment group, the mice were treated with doxorubicin for 2 doses. All mice were performed optical imaging by Zn-DPA-PSS794 or Cy5.5-annexin V, respectively and then sacrificed. The tumor was separated and stained by HE. The expression of caspase-3 protein was measured by Western blot.
  
  
  RESULTS
  The IC50 of doxorubicin to OVCAR-8 was 6 μmol/L. The percentage of apoptosis and dead cells was 35% after doxorubicin treatment. In the optical image, photons accumulated in the tumor either by Zn-DPA-PSS794 or Cy 5.5-annexin V in the treatment group. That was negative in the control group. The fluorescence intensity had significant difference between the 2 groups(P<0.001). The nuclei were big and stained with deep color after the cells were stained with HE. The caspase-3 expression was high in the treatment group, while it was low in the control group.
  
  
  CONCLUSIONS
  Zn-DPA-PSS794 as a probe used by optical imaging can monitor the efficiency of doxorubicin to OVCAR-8 xenograft tumor, which is similar to Cy5.5-annexin V.
authors.affiliation: Department of Nuclear Medicine, Xiangya Hospital, Central South University, Changsha 410008, China. hushuo_xy@sina.com
authors.forename: Shuo
authors.initials: S
authors.lastname: Hu
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chai
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Chunan
authors.initials: C
authors.lastname: Yin
authors.forename: Meng
authors.initials: M
authors.lastname: Lei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002232:Carbocyanines; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007259:Infrared Rays; D051379:Mice; D008819:Mice, Nude; D057054:Molecular Imaging; D009942:Organometallic Compounds; D010051:Ovarian Neoplasms / Q000473:pathology; D010847:Picolines; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010847:Picolines; C509592:zinc(II) dipicolylamine; D004317:Doxorubicin