title: Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli.
abstract: Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.
authors.affiliation: Department of Physics of Complex Systems, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Amit
authors.initials: A
authors.lastname: Zeisel
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Köstler
authors.forename: Natali
authors.initials: N
authors.lastname: Molotski
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Tsai
authors.forename: Rita
authors.initials: R
authors.lastname: Krauthgamer
authors.forename: Jasmine
authors.initials: J
authors.lastname: Jacob-Hirsch
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Yoav
authors.initials: Y
authors.lastname: Soen
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
authors.forename: Yosef
authors.initials: Y
authors.lastname: Yarden
authors.forename: Eytan
authors.initials: E
authors.lastname: Domany
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D002460:Cell Line; D015342:DNA Probes / Q000032:analysis; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D023281:Genomics; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D042361:Mammary Glands, Human / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D015233:Models, Statistical; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013268:Stimulation, Chemical; D013997:Time Factors; D014158:Transcription, Genetic; D059467:Transcriptome / Q000235:genetics*
chemical_list: D015342:DNA Probes; D008070:Lipopolysaccharides; D012322:RNA Precursors; D012333:RNA, Messenger; D004815:Epidermal Growth Factor