title: α-Synuclein in the olfactory system of a mouse model of Parkinson's disease: correlation with olfactory projections.
abstract: Olfactory deficits are an early feature of Parkinson's disease (PD). Neuropathologically, α-synucleinopathy (Lewy bodies and neurites) is observed earlier (stage 1) in the olfactory system than in the substantia nigra (stage 3), and this could underlies the early olfactory symptoms. In the present report, we analyzed the distribution of α-synuclein deposits in tertiary olfactory structures (anterior olfactory nucleus, olfactory tubercle, piriform cortex, posterolateral cortical amygdala and lateral entorhinal cortex) of homozygous transgenic mice (aged 2-8 months) overexpressing the human A53T variant of α-synuclein. To address the hypothesis of progressive α-synucleinopathy within the olfactory system, the distribution of α-synuclein was analyzed in conjunction with tracer injections into the main olfactory bulb. The time-course of α-synuclein expression revealed a significant increase in the piriform cortex at the age of 8 months compared to other brain structures. Tracing experiments revealed that olfactory projections are reduced in homozygous as compared to wild type animals. Double-labeling experiments show labeled axonal collaterals of mitral cells entering layer II of the piriform cortex in close proximity to α-synuclein-positive cells. To our knowledge, this is the first study addressing the progression of α-synuclein expression in a vulnerable neuronal pathway in PD.
authors.affiliation: Departamento de Ciencias Médicas, Laboratorio de Neuroplasticidad y Neurodegeneración, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Avda. de Moledores s/n, 13071, Ciudad Real, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ubeda-Bañon
authors.forename: Daniel
authors.initials: D
authors.lastname: Saiz-Sanchez
authors.forename: Carlos
authors.initials: C
authors.lastname: de la Rosa-Prieto
authors.forename: Alino
authors.initials: A
authors.lastname: Martinez-Marcos
mesh_terms: D000679:Amygdala / Q000378:metabolism / Q000473:pathology; D000818:Animals; D022081:Cell Surface Extensions / Q000473:pathology*; D004195:Disease Models, Animal; D018728:Entorhinal Cortex / Q000378:metabolism / Q000473:pathology; D005260:Female; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009434:Neural Pathways / Q000378:metabolism / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism / Q000473:pathology; D009833:Olfactory Pathways / Q000378:metabolism* / Q000473:pathology*; D010300:Parkinson Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D051844:alpha-Synuclein