title: Characteristic fragmentation patterns of trimethylsilyl and trimethylsilyl-oxime derivatives of plant disaccharides as obtained by gas chromatography coupled to ion-trap mass spectrometry.
abstract: The characteristic fragmentation pattern of six reducing and two non reducing type disaccharides-(neohesperidose, acuminose, sambubiose, rutinose, vicianose, primverose, and two arabinosyl-inositols) has been described. These saccharides have not been previously identified by on-line chromatographic techniques. Unambiguous specific characteristics of the TMS (oxime)s such as mass distribution, syn/anti oximes ratios and elution order proved to be associated with their reducing or non reducing character, with their aldosyl property and with the position of their O-glycosidic linkages. The practical utility of the mass fragmentation study of these rare disaccharides was demonstrated, at the first time, by the simultaneous, on-line identification and quantification of the acuminose, vicianose, primverose and arabinosyl-inositol contents of tea leaves, from green and black tea blends of Indian and Chinese origin.
authors.affiliation: L. Eötvös University, Institute of Biology, Department of Plant Anatomy, Pázmány Péter sétány 1C, 1117 Budapest, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Boldizsár
authors.forename: Zs
authors.initials: Z
authors.lastname: Füzfai
authors.forename: I
authors.initials: I
authors.lastname: Molnár-Perl
mesh_terms: D028241:Camellia sinensis / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010091:Oximes / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D014297:Trimethylsilyl Compounds / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D010091:Oximes; D010936:Plant Extracts; D014297:Trimethylsilyl Compounds