title: [Affection of acupotomy lysis on leu-enkephalin (L-ENK) content in different parts of centrum of rats with knee osteoarthritis].
abstract: OBJECTIVE
  To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin (L-ENK) content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.
  
  
  METHODS
  Sixty healthy SD rats were randomly devided into normal control group, model group, needle-knife lysis (NKL) group and electro-acupuncture (EA) group. 4% papain solution mixed with 0.3 mol/L cysteine solutin in the ratio of 1:1, paused for 0.5 h,injected the mixture, 20 microl each time,into the left knee joint cavities of rats in model, NKL, EA groups at the 1st, 4th, 7th day. After 4 weeks in NKL group and EA group were treated with needle-knife lysis and electro-acupuncture, respectively. Three weeks after treatment, samples of spinal cord of the swollen part of rat waists and rat brains were taken from and the content of L-ENK of medulla oblongata, midbrain, pituitary gland, and hippocampus were measured.
  
  
  RESULTS
  L-ENK content of model group increased higher than that of normal control group in spinal cord, hippocampus and midbrain (P < 0.01); there were no significant difference between normal control group and modle group on L-ENK in medulla oblongata and thalamus (P > 0.05). After intervening of NKL or EA, L-ENK content of NKL group increased higher in hippocampus than that of model group and EC group (P < 0.05); but L-ENK content of NKL group in midbrain was lower than that of model group (P < 0.05).
  
  
  CONCLUSIONS
  Needle-knife lysis has characteristic of regulation for the L-ENK content in different parts of central nervous system of rats with knee osteoarthritis, and analgesic effect of needle-knife was possibly related with regulation of center L-ENK.
authors.affiliation: College of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China. guochangqing66@163.com
authors.forename: Chang-qing
authors.initials: CQ
authors.lastname: Guo
authors.forename: Bo
authors.initials: B
authors.lastname: Ji
authors.forename: You-nan
authors.initials: YN
authors.lastname: Chen
authors.forename: Ding-wen
authors.initials: DW
authors.lastname: Zhong
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Liu
authors.forename: Meng-wei
authors.initials: MW
authors.lastname: Guo
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Nai-gang
authors.initials: NG
authors.lastname: Liu
authors.forename: Zhan-lu
authors.initials: ZL
authors.lastname: Chen
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D002490:Central Nervous System / Q000737:chemistry*; D015671:Electroacupuncture; D004743:Enkephalin, Leucine / Q000032:analysis*; D005260:Female; D008297:Male; D020370:Osteoarthritis, Knee / Q000378:metabolism / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004743:Enkephalin, Leucine