title: Dissemination of IMP-6 metallo-β-lactamase-producing Pseudomonas aeruginosa sequence type 235 in Korea.
abstract: OBJECTIVE
  To investigate the epidemiological traits of Pseudomonas aeruginosa clinical isolates producing metallo-β-lactamases (MBLs) in Korea.
  
  
  METHODS
  A total of 386 non-duplicate P. aeruginosa clinical isolates were collected from Korea in 2009. Detection of MBL genes was performed by PCR. The genetic organization of class 1 integrons carrying the MBL gene cassette was investigated by PCR mapping and sequencing. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and PFGE.
  
  
  RESULTS
  Of 386 P. aeruginosa isolates, 30 (7.8%) isolates carried the bla(IMP-6) gene and 1 (0.3%) isolate carried the bla(VIM-2) gene. A probe specific for the bla(IMP-6) gene was hybridized to an ∼950 kbp I-CeuI-macrorestriction fragment from all 30 isolates and a probe specific for the bla(VIM-2) gene also hybridized to an ∼500 kbp I-CeuI-macrorestriction fragment from 1 isolate (BDC10). All 31 MBL-producing isolates shared an identical sequence type (ST), ST235, and they carried the same bla(OXA-50) allelic type, bla(OXA-50g). All MBL-producing isolates showed similar XbaI-macrorestriction patterns (similarity >85%), irrespective of MBL genotype.
  
  
  CONCLUSIONS
  P. aeruginosa ST235 carrying the chromosomally located bla(IMP-6) gene is widely disseminated in Korea.
authors.affiliation: Department of Laboratory Medicine and Research Institute of Bacterial Resistance,  Yonsei University College of Medicine, Seoul,  Korea.
authors.forename: Yoonmi
authors.initials: Y
authors.lastname: Seok
authors.forename: Il Kwon
authors.initials: IK
authors.lastname: Bae
authors.forename: Seok Hoon
authors.initials: SH
authors.lastname: Jeong
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hyukmin
authors.initials: H
authors.lastname: Lee
authors.forename: Kyungwon
authors.initials: K
authors.lastname: Lee
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D056910:Republic of Korea / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases