title: The use of mouse models to investigate shear bond strength in amelogenesis imperfecta.
abstract: Patients with amelogenesis imperfecta (AI) have defective enamel; therefore, bonded restorations of patients with AI have variable success rates. To distinguish which cases of AI may have good clinical outcomes with bonded materials, we evaluated etching characteristics and bond strength of enamel in mouse models, comparing wild-type (WT) with those having mutations in amelogenin (Amelx) and matrix metalloproteinase-20 (Mmp20), which mimic 2 forms of human AI. Etched enamel surfaces were compared for roughness by scanning electron microscopy (SEM) images. Bonding was compared through shear bond strength (SBS) studies with 2 different systems (etch-and-rinse and self-etch). Etched enamel surfaces of incisors from Amelx knock-out (AmelxKO) mice appeared randomly organized and non-uniform compared with WT. Etching of Mmp20KO surfaces left little enamel, and the etching pattern was indistinguishable from unetched surfaces. SBS results were significantly different when AmelxKO and Mmp20KO enamel surfaces were compared. A significant increase in SBS was measured for all samples when the self-etch system was compared with the etch-and-rinse system. We have developed a novel system for testing shear bond strength of mouse incisors with AI variants, and analysis of these data may have important clinical implications for the treatment of patients with AI.
authors.affiliation: Department of Anatomy and Cell Biology University of Pennsylvania School of Dental Medicine, Philadelphia, PA 19104, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Pugach
authors.forename: F
authors.initials: F
authors.lastname: Ozer
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Sheth
authors.forename: R
authors.initials: R
authors.lastname: Beasley
authors.forename: A
authors.initials: A
authors.lastname: Resnick
authors.forename: L
authors.initials: L
authors.lastname: Daneshmehr
authors.forename: A B
authors.initials: AB
authors.lastname: Kulkarni
authors.forename: J D
authors.initials: JD
authors.lastname: Bartlett
authors.forename: C W
authors.initials: CW
authors.lastname: Gibson
authors.forename: R G
authors.initials: RG
authors.lastname: Lindemeyer
mesh_terms: D000134:Acid Etching, Dental; D000567:Amelogenesis Imperfecta / Q000235:genetics / Q000503:physiopathology*; D053523:Amelogenin / Q000172:deficiency* / Q000502:physiology; D000818:Animals; D001840:Dental Bonding; D003743:Dental Enamel / Q000378:metabolism / Q000473:pathology*; D003799:Dental Stress Analysis; D004195:Disease Models, Animal; D053519:Matrix Metalloproteinase 20 / Q000172:deficiency* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D033081:Shear Strength; D013499:Surface Properties
chemical_list: D053523:Amelogenin; D053519:Matrix Metalloproteinase 20