title: Effect of oleic, lauric and myristic acids on phenylephrine-induced contractions of isolated rat vas deferens.
abstract: D-004, a lipid extract of Roystonea regia fruits that contains oleic, lauric and myristic acids as major components inhibits alpha1-adrenoreceptors-mediated contractile responses in isolated rat vas deferens and prostate trips; no study has demonstrated a similar effect for oleic, lauric or myristic acids individually. Therefore, the effects of D-004 (250 microg/mL), oleic (100 microg/mL), lauric (50 microg/mL) or myristic (25 microg/mL) acids and their combined effects on phenylephrine (PHE: 10(-7)-10(-4) mol/L) induced contractions has been studied. No treatment changed the basal tone of the preparations, but all inhibited PHE-induced contractions. D-004 produced the highest inhibition, followed by lauric acid, which was more effective than myristic and oleic acids against PHE-induced contractions of control group. D-004 and the mixture of the three acids produced similar inhibitions.
authors.affiliation: Centre of Natural Products, National Centre for Scientific Research 25th Ave, 158 st., P.O 6880, Cubanacáin, Havana City, Cuba.
authors.forename: M L
authors.initials: ML
authors.lastname: Arruzazabala
authors.forename: Y
authors.initials: Y
authors.lastname: Pérez
authors.forename: Y
authors.initials: Y
authors.lastname: Ravelo
authors.forename: V
authors.initials: V
authors.lastname: Molina
authors.forename: D
authors.initials: D
authors.lastname: Carbajal
authors.forename: R
authors.initials: R
authors.lastname: Mas
authors.forename: E
authors.initials: E
authors.lastname: Rodríguez
mesh_terms: D058668:Adrenergic alpha-1 Receptor Antagonists / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D028023:Arecaceae; D066298:In Vitro Techniques; D007850:Lauric Acids / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D019814:Myristic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D019301:Oleic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011470:Prostatic Hyperplasia / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014649:Vas Deferens / Q000187:drug effects* / Q000378:metabolism
chemical_list: D058668:Adrenergic alpha-1 Receptor Antagonists; C504503:D 004 extract; D007850:Lauric Acids; D010936:Plant Extracts; D019814:Myristic Acid; C030358:lauric acid; D010656:Phenylephrine; D019301:Oleic Acid