title: Noncovalent complexes of an inactive mutant of CTX-M-9 with the substrate piperacillin and the corresponding product.
abstract: We determined the crystal structure of an inactive Ser70Gly mutant of CTX-M-9 in complex with the bulky penicillin piperacillin at precovalent and posthydrolytic stages in the catalytic process. The structures obtained at high resolution were compared with the corresponding structures for the small penicillin benzylpenicillin and the bulky cephalosporin cefotaxime. The findings highlight the key role of the configuration of the carbon adjacent to the acylamino group of the side chain of β-lactams in the precovalent recognition of substrates.
authors.affiliation: Laboratoire de Bactériologie, CHU Clermont-Ferrand, Clermont-Ferrand F-63003, France.
authors.forename: David
authors.initials: D
authors.lastname: Leyssene
authors.forename: Julien
authors.initials: J
authors.lastname: Delmas
authors.forename: Frédéric
authors.initials: F
authors.lastname: Robin
authors.forename: Antony
authors.initials: A
authors.lastname: Cougnoux
authors.forename: Lucie
authors.initials: L
authors.lastname: Gibold
authors.forename: Richard
authors.initials: R
authors.lastname: Bonnet
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D018360:Crystallography, X-Ray; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010878:Piperacillin / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D047090:beta-Lactams / Q000737:chemistry / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D047090:beta-Lactams; C412613:CTX-M-9 protein, E coli; D001618:beta-Lactamases; D010878:Piperacillin