title: MARCH5 gene is duplicated in rainbow trout, but only fish-specific gene copy is up-regulated after VHSV infection.
abstract: Ubiquitination regulates the activity, stability, and localization of a wide variety of proteins. Several mammalian MARCH ubiquitin E3 ligase proteins have been suggested to control cell surface immunoreceptors. The mitochondrial protein MARCH5 is a positive regulator of Toll-like receptor 7-mediated NF-κB activation in mammals. In the present study, duplicated MARCH5-like cDNA sequences were isolated from rainbow trout (Oncorhynchus mykiss) comprising open reading frames of 882 bp (MARCH5A) and 885 bp (MARCH5B), respectively. Trout MARCH5A and MARCH5B-encoding sequences share only 65% sequence identity. Phylogenetic analyses including an additionally isolated MARCH5-like sequence from whitefish (Coregonus maraena) suggest that teleosts possess an additional MARCH5 gene copy resulting from a fish-specific whole genome duplication. Coding sequences of MARCH5A and MARCH5B genes from trout are distributed over six exons. Hypothetical MARCH5 proteins from trout comprise four transmembrane helices and a single motif similar to a RING variant domain (RINGv) including eight highly conserved cysteine and histidine residues. A 'reverse-northern blot' analysis revealed furthermore a MARCH5B Δexon5 transcript variant. Both MARCH5 genes from trout show a strain-, tissue- and cell-specific expression profile indicating different functional roles. Fish-specific MARCH5A gene for instance might be involved in defense mechanisms, since in vivo-challenge with the viral pathogen VHSV caused a significant 1.7-fold elevated copy number of the respective gene in gills four days after infection, whereas MARCH5B transcript level did not increase.
authors.affiliation: Leibniz-Institut für Nutztierbiologie (FBN), Fachbereich Molekularbiologie, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Rebl
authors.forename: Judith M
authors.initials: JM
authors.lastname: Köbis
authors.forename: Uwe
authors.initials: U
authors.lastname: Fischer
authors.forename: Fumio
authors.initials: F
authors.lastname: Takizawa
authors.forename: Marieke
authors.initials: M
authors.lastname: Verleih
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
authors.forename: Tom
authors.initials: T
authors.lastname: Goldammer
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D005393:Fish Diseases / Q000276:immunology* / Q000821:virology*; D005434:Flow Cytometry; D040461:Gene Components; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology*; D020131:Genes, Duplicate / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D029141:Novirhabdovirus; D017686:Oncorhynchus mykiss; D060888:Real-Time Polymerase Chain Reaction; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D051199:Toll-Like Receptor 7 / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins; D016328:NF-kappa B; D051199:Toll-Like Receptor 7; D044767:Ubiquitin-Protein Ligases