title: Screening of the Salmonella paratyphi A CMCC 50973 strain outer membrane             proteins for the identification of potential vaccine targets.
abstract: Outer membrane protein antigens usually have strong immunogenicities, closely             interact with the immune system and play a significant role in the development             of new vaccines. The outer membrane proteins of Salmonella paratyphi A (S. paratyphi             A) were screened for immunogenicity and immunoprotection for potential vaccine             targets. In this study, the bactericidal effect of antiserum against the total             outer membrane proteins of S. paratyphi A CMCC 50973 strain was determined, and             their immunoprotection was detected with a challenge experiment on vaccinated             mice. The immunogenic outer membrane proteins were identified via immunoproteomic             technology, and recombinant outer membrane proteins were expressed and purified.             The immunoprotection provided by the immunogenic membrane proteins was verified             through active and passive immunity challenge experiments. The result revealed             a number of S. paratyphi A outer membrane proteins that were proven as strong             protective antigens. Twelve immunogenic outer membrane proteins were located and             identified. Five recombinant proteins (LamB, pagC, TolC, nmpC and fadL) with strong             immunoprotective abilities were found via the active immunity challenge experiment,             with protection rates of 95, 95, 85, 80 and 70%, respectively. They were also             proven to induce good immunoprotection via the passive immunity challenge experiment,             with protection rates of 65, 55, 60, 55 and 50%, respectively. The immunoprotective             rate of the five-antiserum combination was 85%. In conclusion, the LamB, pagC,             TolC, nmpC and fadL outer membrane proteins, with strong immunogenicities and             immunoprotection, are effective protein candidate targets for the development             of new vaccines, whereas the recombinant outer membrane proteins are a promising             tool for improving immunoprotection.
authors.affiliation: Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, Xiamen 361004, PR China.
authors.forename: Tian-Ci
authors.initials: TC
authors.lastname: Yang
authors.forename: Xian-Cang
authors.initials: XC
authors.lastname: Ma
authors.forename: Fan
authors.initials: F
authors.lastname: Liu
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Lin
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Liu
authors.forename: Gui-Li
authors.initials: GL
authors.lastname: Liu
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Tong
authors.forename: Zuo-Gen
authors.initials: ZG
authors.lastname: Fu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D022562:Salmonella Vaccines / Q000276:immunology*; D012482:Salmonella paratyphi A / Q000235:genetics* / Q000276:immunology*
chemical_list: D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; D022562:Salmonella Vaccines