title: Subcutaneous connective tissue response to primary root canal filling materials.
abstract: This study evaluated the response of the subcutaneous connective tissue of BALB/c mice to root filling materials indicated for primary teeth: zinc oxide/eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO) and Sealapex sealer. The mice (n=102) received polyethylene tube implants with the materials, thereby forming 11 groups, as follows: I, II, III: Calen/ZO for 7, 21 and 63 days, respectively; IV, V, VI: Sealapex for 7, 21 and 63 days, respectively; VII, VIII, IX: ZOE for 7, 21 and 63 days, respectively; X and XI: empty tube for 7 and 21 days, respectively. The biopsied tissues were submitted to histological analysis (descriptive analysis and semi-quantitative analysis using a scoring system for collagen fiber formation, tissue thickness and inflammatory infiltrate). A quantitative analysis was performed by measuring the area and thickness of the granulomatous reactionary tissue (GRT). Data were analyzed by Kruskal-Wallis, ANOVA and Tukey's post-hoc tests (?=0.05). There was no significant difference (p>0.05) among the materials with respect to collagen fiber formation or GRT thickness. However, Calen/ZO produced the least severe inflammatory infiltrate (p<0.05). The area of the GRT was significantly smaller (p<0.05) for Calen/ZO and Sealapex. In conclusion, Calen/ZO presented the best tissue reaction, followed by Sealapex and ZOE.
authors.affiliation: Department of Pediatric Clinics, Preventive and Community Dentistry, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, SP, Brazil. amqueiroz@forp.usp.br
authors.forename: Alexandra Mussolino de
authors.initials: AM
authors.lastname: Queiroz
authors.forename: Sada
authors.initials: S
authors.lastname: Assed
authors.forename: Alberto
authors.initials: A
authors.lastname: Consolaro
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.forename: Mario Roberto
authors.initials: MR
authors.lastname: Leonardo
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: Silva
authors.forename: Lea Assed Bezerra
authors.initials: LA
authors.lastname: Silva
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001706:Biopsy; D002126:Calcium Hydroxide / Q000494:pharmacology; D003094:Collagen; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D015743:Giant Cells, Foreign-Body / Q000473:pathology; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000473:pathology; D020959:Polyethylene; D011897:Random Allocation; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012459:Salicylates / Q000494:pharmacology; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D014094:Tooth, Deciduous; D015034:Zinc Oxide / Q000494:pharmacology; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C420643:calen; C055601:Sealapex; D020959:Polyethylene; D003094:Collagen; D002126:Calcium Hydroxide; D015034:Zinc Oxide