title: Mixed-phase synthesis of glycopeptides using a N-peptidyl-2,4-dinitrobenzenesulfonamide-thioacid ligation strategy.
abstract: A strategy for the solid phase peptide synthesis (SPPS) and coupling of N-peptidyl and N-glycopeptidyl 2,4-dinitrobenzenesulfonamides (dNBS) with C-terminal peptidyl thioacids has been developed. The resulting N-dDNBS peptides were coupled to generate longer peptides. Ligation reactions were complete within 15 to 20 min.
authors.affiliation: Department of Chemistry, The University of Toledo, 2801 West Bancroft Street, Toledo, Ohio 43606, USA.
authors.forename: Partha
authors.initials: P
authors.lastname: Karmakar
authors.forename: Rommel S
authors.initials: RS
authors.lastname: Talan
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Sucheck
mesh_terms: D000143:Acids / Q000737:chemistry; D006020:Glycopeptides / Q000138:chemical synthesis*; D015394:Molecular Structure; D009578:Nitrobenzenes / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013449:Sulfonamides / Q000737:chemistry*
chemical_list: D000143:Acids; D006020:Glycopeptides; D009578:Nitrobenzenes; D013438:Sulfhydryl Compounds; D013449:Sulfonamides; C036077:nitrobenzene