title: Detection of BCR-ABL fusion proteins in patients with leukemia using a cytometric bead array.
abstract: Rapid and accurate detection of BCR-ABL fusion proteins is vital for the diagnosis and classification of leukemias, especially chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). Recently, the cytometric bead array (CBA) system has been used to identify BCR-ABL proteins in leukemic cell lysates. To evaluate the accuracy of this new technique, we tested 70 patients with hematological diseases using the CBA system, cytogenetic analysis, fluorescence in situ hybridization (FISH) and real-time quantitative polymerase chain reaction (RQ-PCR). Most of the results obtained by the CBA system were concordant with those by cytogenetic analysis, FISH and RQ-PCR. In the CBA system, dilution experiments with positive control samples revealed sensitivities of at least 1%. The statistical analysis revealed that the mean fluorescence intensity (MFI) values of the BCR-ABL fusion proteins from mononuclear cells (MNCs) were higher than those from WBC or BM samples. The MFI values of cells were not dramatically reduced after 24 hours of storage at room temperature. The CBA technique detected all types of BCR-ABL proteins in leukemic cells with high specificity and sensitivity. Therefore, CBAs will contribute to the fast and easy diagnosis of leukemias, the classification of leukemias and the monitoring of minimal residual disease (MRD).
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, Jiangsu, China. wuyujie123456@163.com
authors.forename: Wu
authors.initials: W
authors.lastname: Yujie
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Sixuan
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.forename: Liu
authors.initials: L
authors.lastname: Peng
authors.forename: Ge
authors.initials: G
authors.lastname: Zeng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Sujiang
authors.forename: Li
authors.initials: L
authors.lastname: Jianyong
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood / Q000276:immunology; D001854:Bone Marrow Cells / Q000737:chemistry; D042241:Early Diagnosis; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000379:methods*; D016044:Fusion Proteins, bcr-abl / Q000032:analysis* / Q000097:blood / Q000276:immunology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D007962:Leukocytes / Q000737:chemistry; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry; D011087:Polycythemia Vera / Q000097:blood / Q000235:genetics / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013048:Specimen Handling; D013920:Thrombocythemia, Essential / Q000097:blood / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl