title: [Combined effects of platelet-derived growth factor-BB and insulin-like growth factor-I on the expression of integrin (beta 3 protein in periodontal ligament cells of rats during orthodontic tooth movement].
abstract: OBJECTIVE
  To investigate the combined effects of platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-I (IGF-I) on the expression of integrin (beta3 protein in the periodontal tissues of the compressing side of orthodontic tooth in rats.
  
  
  METHODS
  Establishing orthodontic movement model in Sprague-Dawley rats, which were injected with 10 ng PDGF-BB, 200 ng IGF-I alone or in combination in the buccal submucosal area of the orthodontic tooth. The injection was applied every other day. The experiment continued for ten days and then the rats were sacrificed. The expression of integrin (beta3 protein in periodontal ligament tissues of the compressing side was detected by immunohistochemical techniques.
  
  
  RESULTS
  The expression of integrin (beta3 protein in periodontal ligament tissues of the compressing side of each experimental group was significantly increased compared with that of the control group (P<0.01). Meanwhile the maximum mean optical density value of integrin (beta3 protein expression was attained in the combination group which showed a significant increase compared with the PDGF-BB group (P<0.05) and the IGF-I group (P<0.01).
  
  
  CONCLUSIONS
  The combination of exogenous PDGF-BB and IGF- I in orthodontic tooth movement may enhance the expression of integrin (beta3 protein in periodontal ligament cells and PDGF-BB and IGF-I may have a synergistic effect during orthodontic tooth movement.
authors.affiliation: Dept. of Orthodontics, School of Stomatology, Zunyi Medical College, Zunyi 563003, China.
authors.forename: Mu
authors.initials: M
authors.lastname: Su
authors.forename: Jianguo
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guan
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Xu
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Zhang
authors.forename: Juxiang
authors.initials: J
authors.lastname: Peng
authors.forename: Gaohua
authors.initials: G
authors.lastname: Li
authors.forename: Jianli
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000077214:Becaplermin; D002478:Cells, Cultured; D005347:Fibroblasts; D007334:Insulin-Like Growth Factor I; D016023:Integrins; D010513:Periodontal Ligament; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013002:Somatomedins; D014087:Tooth Movement Techniques
chemical_list: D016023:Integrins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D013002:Somatomedins; D000077214:Becaplermin; D007334:Insulin-Like Growth Factor I