title: Increased expression level of Olfactomedin4 in peripheral blood mononuclear cells of pancreatic adenocarcinoma patients.
abstract: OBJECTIVE
  To identify potential new tumor markers in peripheral blood mononuclear cells for pancreatic adenocarcinoma screening.
  
  
  METHODS
  The total mRNA was extracted from the peripheral blood mononuclear cell samples of both pancreatic adenocarcinoma patients (n=5) and healthy donors (n=5). Having the RNA expression profiling of over 38500 genes via the Human Genome U133 Plus 2.0 Array performed in order to identify differentially expressed genes, the study underwent real-time Reverse Transcriptase PCR (RT-PCR) for validation.
  
  
  RESULTS
  The mRNA expression profiles identified the Olfactomedin4 gene, which was significantly overexpressed (4.46-fold) versus the control group (p<0.01). Afterwards, this result was validated by quantitative RT-PCR, with the same expression trend (4.80-fold) as well as statistical significance (p<0.05).
  
  
  CONCLUSIONS
  Homo sapiens olfactomedin4 was revealed to be up-regulated as a cancer associated gene in peripheral blood mononuclear cells from pancreatic adenocarcinoma patients. Thus, Olfactomedin4, as well as its expression products, might promisingly prove to be the ideal screening tool and new tumor marker available in blood samples.
authors.affiliation: Department of Surgery, Zhejiang University, Zhejiang, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Yan
authors.forename: Di
authors.initials: D
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Xie
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.forename: Yulian
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000097:blood*; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D018395:CA-19-9 Antigen / Q000097:blood; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood* / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; C454127:OLFM4 protein, human; D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor