title: [Effects of PRL-3 siRNA on the migration of endometriotic stromal cells].
abstract: OBJECTIVE
  To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.
  
  
  METHODS
  Primary endometriotic stromal cells were cultured in vitro. Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene. And the PRL-3 protein expression was analyzed by Western blot. The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.
  
  
  RESULTS
  The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P < 0.05). The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87 ± 0.21 in experimental group. It was less than 1.75 ± 0.28 in blank control group and 1.63 ± 0.39 in negative control group (P < 0.05).
  
  
  CONCLUSIONS
  PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells. And PRL-3 may be a promising target in the therapeutics of endometriosis.
authors.affiliation: Department of Obstetrics & Gynecology, Zhejiang University, Hangzhou 310006, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ruan
authors.forename: Rui-jin
authors.initials: RJ
authors.lastname: Wu
authors.forename: Kai-hong
authors.initials: KH
authors.lastname: Xu
authors.forename: Jun-yan
authors.initials: JY
authors.lastname: Ma
authors.forename: Dong-hong
authors.initials: DH
authors.lastname: Lu
authors.forename: Li-qing
authors.initials: LQ
authors.lastname: Chen
authors.forename: Fei-xia
authors.initials: FX
authors.lastname: Tu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
mesh_terms: D000328:Adult; D002465:Cell Movement; D002478:Cells, Cultured; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D014162:Transfection; D055815:Young Adult
chemical_list: D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases