title: Dehydroabietic acid activates peroxisome proliferator-activated receptor-γ and stimulates insulin-dependent glucose uptake into 3T3-L1 adipocytes.
abstract: Dehydroabietic acid (DAA) is a food-derived terpenoid with various bioactivities. Our previous study has revealed that DAA activates peroxisome proliferator-activated receptor-γ (PPARγ) in luciferase assay and suppresses chronic inflammation in obese adipose tissues. In this study, we examined the effects of DAA on adipocyte differentiation. DAA treatment stimulated the adipocyte differentiation of 3T3-L1 preadipocytes. The DAA treatment increased the mRNA expression levels of adipocyte differentiation marker genes such as aP2, lipoprotein lipase (LPL), and PPARγ. In particular, the expression level of adiponectin, which is an adipocytokine with stimulatory effects on insulin sensitivity, was increased at both the mRNA and protein levels by the DAA treatment. Moreover, the DAA treatment stimulated insulin-dependent glucose uptake into differentiated 3T3-L1 adipocytes. These findings indicate that DAA stimulates adipocyte differentiation and insulin sensitivity in 3T3-L1 cells, suggesting that DAA is a valuable food-derived compound for the management of metabolic syndrome.
authors.affiliation: Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takahashi
authors.forename: Ran
authors.initials: R
authors.lastname: Yao
authors.forename: Min-Sook
authors.initials: MS
authors.lastname: Kang
authors.forename: Mari
authors.initials: M
authors.lastname: Senda
authors.forename: Chieko
authors.initials: C
authors.lastname: Ando
authors.forename: Kanako
authors.initials: K
authors.lastname: Nishimura
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.forename: Shizuka
authors.initials: S
authors.lastname: Hirai
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Ezaki
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
mesh_terms: D041721:3T3-L1 Cells; D045784:Abietanes / Q000494:pharmacology*; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D045784:Abietanes; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D012333:RNA, Messenger; C013913:dehydroabietic acid; D008071:Lipoprotein Lipase