title: Resveratrol attenuates acute hypoxic injury in cardiomyocytes: correlation with inhibition of iNOS-NO signaling pathway.
abstract: OBJECTIVE
  To investigate the effect of resveratrol on hypoxia-induced cardiomyocytes injury and its potential mechanism.
  
  
  METHODS
  Cell injury was evaluated by supernatant lactate dehydrogenase (LDH) assay and cell viability. NO production in cardiomyocytes were assessed by Griess reagent, expressions of inducible nitric oxide synthase (iNOS) and hypoxia-inducible factor 1 (HIF-1α) were determined reverse transcription polymerase chain reaction (RT-PCR). HIF-1α protein was determined by Western blot.
  
  
  RESULTS
  Hypoxia increased LDH release, decreased cell viability. Pretreatment with resveratrol (10, 25, and 50μM) decreased LDH release and increased cell viability. Resveratrol suppressed the mRNA expression of iNOS as well as NO production in hypoxia-induced cardiomyocytes. Consistent with the inhibitory effect on iNOS protein expression, resveratrol inhibited iNOS mRNA expression. Resveratrol also inhibited HIF-1α mRNA expression.
  
  
  CONCLUSIONS
  These results suggested that resveratrol attenuates acute hypoxic injury in cardiomyocytes, and the mechanism might be associated with inhibition of iNOS-NO signaling pathway via HIF-1α.
authors.affiliation: Institute of Molecular Biology & Translational Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, Jiangsu 212002, China.
authors.forename: Shijun
authors.initials: S
authors.lastname: Wang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Qian
authors.forename: Dandan
authors.initials: D
authors.lastname: Gong
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002316:Cardiotonic Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques; D015687:Cell Hypoxia / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D002316:Cardiotonic Agents; D003470:Culture Media; D013267:Stilbenes; D009569:Nitric Oxide; D045022:Lactate Dehydrogenases; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D000077185:Resveratrol