title: Sample preparation for small RNA massive parallel sequencing.
abstract: High-throughput sequencing has allowed for a comprehensive small RNA (sRNA) expression analysis of numerous tissues in a diverse set of organisms. The computational analysis of the millions of generated sequencing reads has led to the discovery of novel miRNAs and other sRNA species, and resulted in a better understanding of the roles these sRNAs play in development and disease. This chapter describes the generation of sRNA deep-sequencing libraries for the Illumina massively parallel sequencing platform by using a cloning method that anneals specific RNA sequences to the 5'- and 3'-ends of the sRNA molecules.
authors.affiliation: InteRNA Technologies BV, Utrecht, The Netherlands. gommans@interna-technologies.com
authors.forename: Willemijn M
authors.initials: WM
authors.lastname: Gommans
authors.forename: Eugene
authors.initials: E
authors.lastname: Berezikov
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000235:genetics*
chemical_list: D058727:RNA, Small Untranslated