title: Characterization of Lactobacillus isolates from fermented olives and their bacteriocin gene profiles.
abstract: Near one hundred isolates of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum from table olives were studied. Strains were genotyped by rep-PCR. Although the technique failed to differentiate some isolates at the species level, it proved a robust and easy procedure that could be useful for distinguishing between related strains of L. paraplantarum, L. pentosus and L. plantarum from a large pool of unrelated strains of these species. A PCR-based screening revealed the presence of the plantaricin encoding genes plnA, plnB, plnC, plnD, plnE/F, plnF, plnI, plnJ, plnK, plnG and plnN in most isolates of the three species. Sequences of bacteriocin genes present in L. paraplantarum and L. pentosus were homologous to L. plantarum genes. Through a discriminating analysis of the bacteriocin gene profiles, it was possible to establish a relationship between the origin of isolation and the LAB isolates, regardless of species.
authors.affiliation: Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Campus Sescelades, 43007 Tarragona, Catalonia, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Hurtado
authors.forename: Nada
authors.initials: N
authors.lastname: Ben Othman
authors.forename: Nadia
authors.initials: N
authors.lastname: Chammem
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
authors.forename: Sergi
authors.initials: S
authors.lastname: Ferrer
authors.forename: Cristina
authors.initials: C
authors.lastname: Reguant
authors.forename: Albert
authors.initials: A
authors.lastname: Bordons
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rozès
mesh_terms: D001430:Bacteriocins / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D031658:Olea / Q000378:metabolism / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D001430:Bacteriocins