title: Gene polymorphisms of adiponectin and leptin receptor are associated with early onset of type 2 diabetes mellitus in the Taiwanese population.
abstract: OBJECTIVE
  Adipocytokine genes encoding adiponectin (ADIPOQ) and the leptin receptor (LEPR) affect glucose and fatty acid metabolism. The purpose of this study was to examine the association between early-onset type 2 diabetes mellitus (T2DM) and variability within these two genes in the Han Chinese population of Taiwan.
  
  
  METHODS
  A cross-sectional study of 999 patients from the Han Chinese population of Taiwan with early-onset T2DM (n=264; age at diagnosis, 20 to <45 years) and late-onset T2DM (n=735; age at diagnosis, ~45 years) was performed. Blood samples from T2DM patients were taken for DNA extraction, and levels of serological markers were measured at enrollment. Seven single-nucleotide polymorphisms (SNPs) were selected for genotyping (three SNPs in AIDPOQ and four SNPs in LEPR) by polymerase chain reaction in each patient.
  
  
  RESULTS
  Polymorphisms at the position rs10937273 in ADIPOQ and at the positions rs1892534 and rs2211651 in LEPR were statistically associated with early-onset T2DM (P=0.0246, 0.0014 and 0.0012, respectively). C-reactive protein levels were significantly different among the early-onset T2DM patients with different genotypes at the SNPs rs1892534 and rs2211651 in LEPR (P=0.003 and P=0.004, respectively). In addition, fasting glucose levels were also significantly different among different genotypes at the SNP rs1892534 in LEPR (P=0.038).
  
  
  CONCLUSIONS
  We conclude that the polymorphisms in the adipocytokine genes ADIPOQ and LEPR are significantly associated with the age at diagnosis of T2DM in the Han Chinese population of Taiwan.
authors.affiliation: Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
authors.forename: W-L
authors.initials: WL
authors.lastname: Liao
authors.forename: C-C
authors.initials: CC
authors.lastname: Chen
authors.forename: C-T
authors.initials: CT
authors.lastname: Chang
authors.forename: J-Y
authors.initials: JY
authors.lastname: Wu
authors.forename: C-H
authors.initials: CH
authors.lastname: Chen
authors.forename: Y-C
authors.initials: YC
authors.lastname: Huang
authors.forename: C-H
authors.initials: CH
authors.lastname: Tsai
authors.forename: F-J
authors.initials: FJ
authors.lastname: Tsai
mesh_terms: D052242:Adiponectin / Q000097:blood*; D000328:Adult; D017668:Age of Onset; D044466:Asian People / Q000235:genetics*; D001786:Blood Glucose / Q000378:metabolism*; D002097:C-Reactive Protein / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000453:epidemiology / Q000235:genetics; D005227:Fatty Acids / Q000097:blood; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006442:Glycated Hemoglobin / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000097:blood*; D013624:Taiwan / Q000453:epidemiology
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D002097:C-Reactive Protein; D002784:Cholesterol; D005227:Fatty Acids; D006442:Glycated Hemoglobin; D054411:Receptors, Leptin; C517652:hemoglobin A1c protein, human