title: Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions.
abstract: The aim of this study was to assess the immunohistochemical expression of syndecan-1 (CD138) and Ki-67 in radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). Thirty-five RC, 22 DC and 17 KOT were used in the study and immunohistochemical reactions using anti-syndecan-1 and anti-Ki-67 antibodies were performed by the streptavidin-biotin-peroxidase method. Fisher's exact test and Spearman's correlation coefficient were used for statistical analysis of data. Among the studied lesions, no differences in the syndecan-1 expression were observed, but the suprabasal expression of Ki-67 was significantly higher in KOT (p<0.0001), when compared with RC and DC. In RC, there was positive correlation between the expression (p=0.02) and intensity (p=0.0001) of syndecan-1 and between the intensity of syndecan-1 and Ki-67 expression (p=0.01). In the KOT, Ki-67 expression in the suprabasal layer correlated positively with the expression (p=0.01) and intensity (p=0.01) of syndecan-1. The expression of syndecan-1 does not seem to be a determinant factor of the distinct histopathological features and biological behavior of the studied lesions. Nevertheless, positive correlation between syndecan-1 and a cell proliferation marker was observed in RC and KOT.
authors.affiliation: Dental School, University of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors.forename: Michele Regina
authors.initials: MR
authors.lastname: Nadalin
authors.forename: Eduardo Rodrigues
authors.initials: ER
authors.lastname: Fregnani
authors.forename: Yara Teresinha Correa
authors.initials: YT
authors.lastname: Silva-Sousa
authors.forename: Danyel Elias da Cruz
authors.initials: DE
authors.lastname: Perez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies; D049109:Cell Proliferation; D002648:Child; D003803:Dentigerous Cyst / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008336:Mandibular Diseases / Q000473:pathology; D008339:Mandibular Neoplasms / Q000473:pathology; D008439:Maxillary Diseases / Q000473:pathology; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000473:pathology*; D009808:Odontogenic Tumors / Q000473:pathology*; D011842:Radicular Cyst / Q000473:pathology; D012189:Retrospective Studies; D053668:Syndecan-1 / Q000032:analysis*; D055815:Young Adult
chemical_list: D000906:Antibodies; D019394:Ki-67 Antigen; C508241:SDC1 protein, human; D053668:Syndecan-1