title: The real-time PCR for sensitive protein detection by target-induced intermolecular hybridization.
abstract: An approach was developed to sensitively and specially detect protein in buffer and serum based on target-induced intermolecular hybridization and the significant difference of duplex stability in intramolecular versus intermolecular.
authors.affiliation: State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Ma
authors.forename: Lijie
authors.initials: L
authors.lastname: Cao
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
authors.forename: Naihao
authors.initials: N
authors.lastname: Ye
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002021:Buffers; D009693:Nucleic Acid Hybridization; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013917:Thrombin / Q000032:analysis* / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D002021:Buffers; D013917:Thrombin