title: Resonance scattering spectral detection of trace ATP based on label-free aptamer reaction and nanogold catalysis.
abstract: Double-stranded DNA (dsDNA) cannot protect gold nanoparticles (AuNPs) in the presence of NaCl, and dsDNA interacted with adenosine triphosphate (ATP) to form stable G-quartet and a single-stranded DNA (DNA 2) that can protect AuNPs. The unprotected AuNPs were aggregated to AuNP aggregations (AuNPA) that exhibited a resonance scattering (RS) peak at 590 nm. The RS intensity at 590 nm decreased linearly when the ATP concentration increased in the range of 6.6-110 nM. The catalysis of AuNP-DNA 2 was stronger than that of the AuNPA on the glucose-Cu(II) particle reaction, and the product appeared as an RS peak at 620 nm. When the ATP concentration was increased, the AuNP-DNA 2 increased, and the RS intensity at 620 nm increased linearly. The increased RS intensity (ΔI(620 nm)) was linear to ATP concentration in the range of 2.2-220 nM, with a regression equation of ΔI(620 nm) = 0.709C + 7.7, and a detection limit of 0.5 nM. Hereby, a new RS method of ATP detection was set up with high sensitivity and selectivity.
authors.affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin 541004, China.
authors.forename: Aihui
authors.initials: A
authors.lastname: Liang
authors.forename: Huixiang
authors.initials: H
authors.lastname: Ouyang
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide; D002384:Catalysis; D006046:Gold; D053768:Metal Nanoparticles; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D000255:Adenosine Triphosphate