title: Isolation and characterization of polysaccharides of a hybrid mushroom (backcross mating between PfloVv12 and Volvariella volvacea).
abstract: Three polysaccharide fractions (PS-I, PS-II, and PS-III) were isolated from the aqueous extract of a hybrid mushroom obtained through backcross mating of a somatic hybrid mushroom PfloVv12 (Sterile line) with Volvariella volvacea. PfloVv12 was obtained through protoplast fusion of Pleurotus florida and V. volvacea. PS-I was identified as 1,6-β glucan. PS-II and PS-III were identified as mannoglucogalactan but differing in molecular weights only. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiment ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC) the structures of these polysaccharides were established as;
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.
authors.forename: Chanchal K
authors.initials: CK
authors.lastname: Nandan
authors.forename: Ramsankar
authors.initials: R
authors.lastname: Sarkar
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Bhanja
authors.forename: Samir R
authors.initials: SR
authors.lastname: Sikdar
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002678:Chimera / Q000235:genetics / Q000378:metabolism*; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry; D007178:Inbreeding; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D020076:Pleurotus; D055367:Volvariella
chemical_list: D005685:Galactans; D005936:Glucans; D005947:Glucose; D008358:Mannose