title: [Comparison of the indirect immunofluorescence assay performance of Bartonella henselae antigens obtained by co-cultivation in Vero and HeLa cells].
abstract: The laboratory diagnosis of Bartonella henselae infection is mainly based on serological testing by indirect immunofluorescence assay (IFA). Cell line co-cultivation with B.henselae and agar derivated antigens are the two major procedures used for evaluation of anti-Bartonella antibodies. Vero and Hep-2 cell lines are the most commonly used media for co-cultivation both in-house and commercial diagnostic kits production. However, HeLa cells which are easily supplied and grown, also can easily be infected by B.henselae. The aim of this study was to compare the performances of antigens obtained by co-cultivation of B.henselae ATCC 49882 (Houston-1) in Vero and HeLa Cells in IFA serology. Out of 381 sera samples, 127 (33.3%) were found positive and 195 (51.2%) were found negative by IFA performed by both cell line co-cultivations. The total agreement between the methods were found as 84.5% (322/381), and Cohen kappa value was calculated as 0.68 (strong, coherent). As a result, He-La cells were found to be useful for the preparation of B.henselae antigens to be used in IFA for the serologic diagnosis of B.henselae infections. However different genotype strains and cross reactions with other infectious agents should be investigated by further studies before routine applications of HeLa cell co-cultivations procedure is established.
authors.affiliation: Pamukkale University Faculty of Medicine, Department of Medical Microbiology, Denizli, Turkey. cagri@pau.edu.tr
authors.forename: Cağrı
authors.initials: C
authors.lastname: Ergin
authors.forename: Yüksel
authors.initials: Y
authors.lastname: Akkaya
authors.forename: Ozgün
authors.initials: O
authors.lastname: Kiriş Satılmış
authors.forename: Cansev
authors.initials: C
authors.lastname: Yılmaz
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D018416:Bartonella henselae / Q000276:immunology*; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000592:standards*; D006367:HeLa Cells; D006801:Humans; D014709:Vero Cells
chemical_list: D000942:Antigens, Bacterial