title: ChIP-Seq: technical considerations for obtaining high-quality data.
abstract: Chromatin immunoprecipitation followed by next-generation sequencing analysis (ChIP-Seq) is a powerful method with which to investigate the genome-wide distribution of chromatin-binding proteins and histone modifications in any genome with a known sequence. The application of this technique to a variety of developmental and differentiation systems has provided global views of the cis-regulatory elements, transcription factor function and epigenetic processes involved in the control of gene transcription. Here we describe several technical aspects of the ChIP-Seq assay that diminish bias and background noise and allow the consistent generation of high-quality data.
authors.affiliation: Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. benjamin.kidder@nih.gov
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Kidder
authors.forename: Gangqing
authors.initials: G
authors.lastname: Hu
authors.forename: Keji
authors.initials: K
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002452:Cell Count; D002843:Chromatin; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D015723:Gene Library; D051379:Mice; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013223:Statistics as Topic
chemical_list: D000911:Antibodies, Monoclonal; D002843:Chromatin