title: The effect of immersion oil in optical tweezers.
abstract: Optimized optical tweezers are of great importance for biological micromanipulation. In this paper, we present a detailed electromagnetic-based calculation of the spatial intensity distribution for a laser beam focused through a high numerical aperture objective when there are several discontinuities in the optical pathway of the system. For a common case of 3 interfaces we have shown that 0.01 increase in the refractive index of the immersion medium would shift the optimal trapping depth by 3-4 μm (0.2-0.6 μm) for aqueous (air) medium. For the first time, We have shown that the alteration of the refractive index of the immersion medium can be also used in aerosol trapping provided that larger increase in the refractive index is considered.
authors.affiliation: Department of Physics, Institute for Advanced Studies in Basic Sciences, Gava Zang, PO Box 45195-1159, Zanjan 45137-66731, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mahmoudi
authors.forename: S Nader S
authors.initials: SN
authors.lastname: Reihani
mesh_terms: D000336:Aerosols; D000465:Algorithms; D003952:Diagnostic Imaging / Q000379:methods; D060733:Electromagnetic Radiation; D007834:Lasers; D008846:Micromanipulation / Q000379:methods; D015233:Models, Statistical; D016011:Normal Distribution; D052898:Optical Tweezers; D055095:Optics and Photonics; D012031:Refractometry; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D000336:Aerosols; D014867:Water