title: Quantity of clonal cells detected by conventional cytogenetic analysis correlates with bone marrow blasts and survival in myelodysplastic syndromes.
abstract: We performed quantitative and qualitative analyses of conventional cytogenetic analysis and interphase FISH results in 87 MDS patients. The quantity of clonal cells for each chromosome of CCA did not correlate with the result of iFISH (r, range 0.0761-1.0577). The clonal cell percentage in CCA was higher in patients with >5% bone marrow blasts than those with <5% (44.7% vs. 23.1%, p=0.017). Multivariate analysis showed that a high quantity of clonal cells in CCA analysis is an independent prognostic factor for overall survival in MDS (p=0.012).
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Jongno-gu, Seoul, South Korea.
authors.forename: Miyoung
authors.initials: M
authors.lastname: Kim
authors.forename: Soie
authors.initials: S
authors.lastname: Chung
authors.forename: Cha Ja
authors.initials: CJ
authors.lastname: See
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Yoon
authors.forename: Byoung Kook
authors.initials: BK
authors.lastname: Kim
authors.forename: Hyun Kyung
authors.initials: HK
authors.lastname: Kim
authors.forename: Dong Soon
authors.initials: DS
authors.lastname: Lee
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001752:Blast Crisis; D001853:Bone Marrow / Q000473:pathology*; D002869:Chromosome Aberrations; D002999:Clone Cells; D020732:Cytogenetic Analysis; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D011379:Prognosis; D015996:Survival Rate; D055815:Young Adult