title: Protective role of interleukin-19 gene polymorphisms in patients with ulcerative colitis.
abstract: Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) of unknown etiology. Interleukin (IL)-19 belongs to the IL-10 family and is a potent immunomodulatory cytokine, with implications for pathogenesis in IBD. No previous studies have reported this novel association between UC and IL-19 polymorphisms. In the present work, the role of IL-19 gene polymorphisms as susceptibility markers for UC was evaluated. Three polymorphisms of IL-19 gene (rs2243188, rs2243191, and rs2243193) were genotyped by 5' exonuclease TaqMan genotyping assays on an ABI Prism 7900 HT Fast Real-Time PCR System in a group of 200 Mexican Mestizo patients with UC and 698 healthy unrelated individuals with no family history of UC. The rs2243191 polymorphism significantly deviated from Hardy-Weinberg equilibrium (HWE) (p < 0.05) and consequently was not included in the analysis. Although genotypes AA (rs2243188) and AA (rs2243193) were significant decreased in UC patients as compared with healthy controls (p(c) = 0.018 and p(c) = 0.006, respectively), a genetic additive effect for the alleles was not observed (Cochran-Armitage trend test, not significant). In the subgroup analysis, no differences were found between the IL-19 genotypes and the clinical characteristics of UC. The results suggest that IL-19 polymorphisms (rs2243188 and rs2243193) might have a protective role in the development of UC in Mexican individuals.
authors.affiliation: Inflammatory Bowel Disease Clinic, Department of Gastroenterology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
authors.forename: Jesús K
authors.initials: JK
authors.lastname: Yamamoto-Furusho
authors.forename: Edith
authors.initials: E
authors.lastname: Álvarez-León
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Fragoso
authors.forename: Anna
authors.initials: A
authors.lastname: Gozalishvilli
authors.forename: Maite
authors.initials: M
authors.lastname: Vallejo
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vargas-Alarcón
mesh_terms: D000328:Adult; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D056747:Immunomodulation; D007378:Interleukins / Q000235:genetics*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012306:Risk
chemical_list: C421250:IL19 protein, human; D007378:Interleukins