title: Lamivudine resistance mutations in European patients with hepatitis B and patients co-infected with HIV and hepatitis B.
abstract: Evaluation of resistance pattern in patients with chronic hepatitis B. Retrospective study of hepatitis B virus (HBV) resistance mutations in patients found viraemic after first-line treatment. HBV viral load was determined by a real-time polymerase chain reaction and the substitutions in HBV-DNA were studied by polymerase sequencing test. First line treatment had failed in 12 out of 33 patients (36%) receiving anti-HBV drugs. The 12 patients with persistent viraemia were all lamivudine (LAM) experienced and 7 had a polymerase sequencing test available. LAM substitution mutations L180M + M204V/I were found in six out of seven cases, with an accompanying V173L mutation in three cases. These mutations were also related with changes in HBsAg. The use of potent drugs in the first line anti-HBV therapy may reduce the resistance mutations in the future.
authors.affiliation: Infectious Diseases Department, San Martino Hospital and University of Genoa, Genoa, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Taramasso
authors.forename: Patrizia
authors.initials: P
authors.lastname: Caligiuri
authors.forename: Antonio
authors.initials: A
authors.lastname: Di Biagio
authors.forename: Bianca
authors.initials: B
authors.lastname: Bruzzone
authors.forename: Raffaella
authors.initials: R
authors.lastname: Rosso
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Icardi
authors.forename: Claudio
authors.initials: C
authors.lastname: Viscoli
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral; D005260:Female; D015658:HIV Infections / Q000150:complications; D006515:Hepatitis B virus / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000150:complications / Q000821:virology*; D006801:Humans; D019259:Lamivudine / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D012189:Retrospective Studies; D017211:Treatment Failure; D019562:Viral Load; D014766:Viremia
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D019259:Lamivudine