title: Analysis of CD177 neutrophil expression in β-thalassemia patients.
abstract: Ineffective erythropoiesis plays a well established role in the pathophysiology of disease expression in β-thalassemia major and intermedia. CD177 expression was investigated in different clinical conditions. The study aimed to analyze neutrophil expression of CD177 in β-thalassemia patients and its correlation with serum soluble transferrin receptor (s-TfR) concentration as a marker for the extent of erythropoiesis and hence disease severity in these patients. Flow cytometric analysis of neutrophil CD177 expression and enzyme immunoassay measurement of serum s-TfR concentration were assessed in 45 β-thalassemia patients of whom 36 had β-thalassemia major and nine had β-thalassemia intermedia. They were also assessed in 21 age- and gender-matched control children. Neutrophil mean fluorescence intensity ratio (MFIR) of CD177 expression was significantly higher in patients than in controls (p < 0.001). There was highly significant increase in serum s-TfR concentration in β-thalassemia patients than in controls (p < 0.001). There was a highly significant positive correlation between MFIR of CD177 expression and serum s-TfR concentration in β-thalassemia patients (r = 0.59, p < 0.001). Elevated CD177 expression is not only a specific feature of polycythemia rubra vera (PV) but may be also an indicator of increased erythropoietic activity in thalassemia syndromes.
authors.affiliation: The Department of Clinical Pathology, Faculty of Medicine, Menofiya University, Shebein ElKom, Egypt.
authors.forename: Laila Mahmoud
authors.initials: LM
authors.lastname: Montaser
authors.forename: Farida Hussein
authors.initials: FH
authors.lastname: El-Rashidi
authors.forename: Enas Said
authors.initials: ES
authors.lastname: Essa
authors.forename: Samar Mostafa
authors.initials: SM
authors.lastname: Azab
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004920:Erythropoiesis / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000097:blood / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007519:Isoantigens / Q000097:blood / Q000276:immunology*; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000097:blood / Q000276:immunology*; D011990:Receptors, Transferrin / Q000097:blood / Q000276:immunology*; D018709:Statistics, Nonparametric; D017086:beta-Thalassemia / Q000097:blood / Q000276:immunology*
chemical_list: C407242:CD177 protein, human; D058851:GPI-Linked Proteins; D007519:Isoantigens; D011956:Receptors, Cell Surface; D011990:Receptors, Transferrin