title: Methylated-CpG Island Recovery Assay.
abstract: Alterations in DNA methylation patterns are implicated in playing a major role in the development of cancer, thus highlighting the need to continually develop new technologies to analyze epigenetic marks. Methylated-CpG Island Recovery Assay (MIRA), based on the high affinity of the MBD2b/MDB3L1 complex for double-stranded methylated DNA, allows for the recovery of methylated DNA without the use of bisulfite conversion or antibody recognition. MIRA is capable of detecting low-density methylation of a single methylated CpG nucleotide. This technique can be used in conjunction with microarrays or next-generation sequencing to analyze recovered methylated DNA on a genome-wide scale.
authors.affiliation: Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Natalie
authors.initials: N
authors.lastname: Mitchell
authors.forename: J Tyson
authors.initials: JT
authors.lastname: Deangelis
authors.forename: Trygve O
authors.initials: TO
authors.lastname: Tollefsbol
mesh_terms: D018899:CpG Islands / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019175:DNA Methylation / Q000235:genetics*; D044127:Epigenesis, Genetic / Q000235:genetics; D005821:Genetic Techniques / Q000295:instrumentation; D008863:Microspheres
chemical_list: D004247:DNA