title: New 30-valent M protein-based vaccine evokes cross-opsonic antibodies against non-vaccine serotypes of group A streptococci.
abstract: Our previous studies have shown that recombinant multivalent vaccines containing amino-terminal M protein fragments from as many as 26 different serotypes of group A streptococci (GAS) evoked opsonic antibodies in animals and humans. In the present study, we constructed a new 30-valent vaccine containing M protein peptides from GAS serotypes prevalent in North America and Europe. The vaccine was immunogenic in rabbits and evoked bactericidal antibodies against all 30 vaccine serotypes of GAS. In addition, the vaccine antisera also contained significant levels of bactericidal antibodies against 24 of 40 non-vaccine serotypes of GAS. These results indicate that the potential efficacy of the new multivalent vaccine may be greater than predicted based on the "type-specific" M peptides represented.
authors.affiliation: Department of Medicine, The University of Tennessee Health Science Center, Memphis, TN 38163, USA. jbdale@uthsc.edu
authors.forename: James B
authors.initials: JB
authors.lastname: Dale
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Penfound
authors.forename: Edna Y
authors.initials: EY
authors.lastname: Chiang
authors.forename: William J
authors.initials: WJ
authors.lastname: Walton
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001770:Blood Bactericidal Activity; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D009895:Opsonin Proteins / Q000097:blood*; D011817:Rabbits; D022541:Streptococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D009895:Opsonin Proteins; D022541:Streptococcal Vaccines; D014614:Vaccines, Synthetic; C014689:streptococcal M protein