title: Feeding blueberry diets in early life prevent senescence of osteoblasts and bone loss in ovariectomized adult female rats.
abstract: BACKGROUND
  Appropriate nutrition during early development is essential for maximal bone mass accretion; however, linkage between early nutrition, childhood bone mass, peak bone mass in adulthood, and prevention of bone loss later in life has not been studied.
  
  
  RESULTS
  In this report, we show that feeding a high quality diet supplemented with blueberries (BB) to pre-pubertal rats throughout development or only between postnatal day 20 (PND20) and PND34 prevented ovariectomy (OVX)-induced bone loss in adult life. This protective effect of BB is due to suppression of osteoblastic cell senescence associated with acute loss of myosin expression after OVX. Early exposure of pre-osteoblasts to serum from BB-fed rats was found to consistently increase myosin expression. This led to maintenance osteoblastic cell development and differentiation and delay of cellular entrance into senescence through regulation of the Runx2 gene. High bone turnover after OVX results in insufficient collagenous matrix support for new osteoblasts and their precursors to express myosin and other cytoskeletal elements required for osteoblast activity and differentiation.
  
  
  CONCLUSIONS
  These results indicate: 1) a significant prevention of OVX-induced bone loss from adult rats can occur with only 14 days consumption of a BB-containing diet immediately prior to puberty; and 2) the molecular mechanisms underlying these effects involves increased myosin production which stimulates osteoblast differentiation and reduces mesenchymal stromal cell senescence.
authors.affiliation: Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, United States of America.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Oxana P
authors.initials: OP
authors.lastname: Lazarenko
authors.forename: Michael L
authors.initials: ML
authors.lastname: Blackburn
authors.forename: Kartik
authors.initials: K
authors.lastname: Shankar
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Badger
authors.forename: Martin J J
authors.initials: MJ
authors.lastname: Ronis
authors.forename: Jin-Ran
authors.initials: JR
authors.lastname: Chen
mesh_terms: D000818:Animals; D029798:Blueberry Plants; D002454:Cell Differentiation; D048430:Cell Shape; D016922:Cellular Senescence; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D004032:Diet; D005260:Female; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009210:Myofibrils / Q000378:metabolism; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology*; D010024:Osteoporosis / Q000097:blood / Q000378:metabolism / Q000473:pathology* / Q000517:prevention & control*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D009218:Myosins