title: A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco.
abstract: WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced quickly and strongly in response to various environmental stresses, including low temperatures, ABA, salt and dehydration. Constitutive expression of BcWRKY46 in tobacco under the control of the CaMV35S promoter reduced the susceptibility of transgenic tobacco to freezing, ABA, salt and dehydration stresses. Our studies suggest that BcWRKY46 plays an important role in responding to ABA and abiotic stress.
authors.affiliation: State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Xilin
authors.initials: X
authors.lastname: Hou
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Shuming
authors.initials: S
authors.lastname: Wang
authors.forename: Fangling
authors.initials: F
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D001937:Brassica / Q000187:drug effects / Q000235:genetics*; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D003681:Dehydration; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D018525:Germination / Q000187:drug effects; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D010641:Phenotype; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics*; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid