title: Broad bean and pea by-products as sources of fibre-rich ingredients: potential antioxidant activity measured in vitro.
abstract: BACKGROUND
  By-products generated during the processing of plant food can be considered a promising source of dietary fibre as a functional compound. The dietary fibre composition, soluble sugars and antioxidant activity of the extractable polyphenols of pea and broad bean by-products have been analysed in this study.
  
  
  RESULTS
  Total dietary fibre using AOAC methods plus hydrolysis (broad bean pod: 337.3 g kg⁻¹; pea pod: 472.6 g kg⁻¹) is higher (P < 0.05) in both by-products than with the Englyst method (broad bean pod: 309.7 g kg⁻¹; pea pod: 434.6 g kg⁻¹). The main monomers are uronic acids, glucose, arabinose and galactose in broad bean pods. However, pea pods are very rich in glucose and xylose. The soluble sugars analysed by high-performance liquid chromatography in both by-products have glucose as the most important component, followed by sucrose and fructose. The ferric reducing antioxidant power (broad bean pod: 406.4 µmol Trolox equivalents g⁻¹; pea pod: 25.9 µmol Trolox equivalents g⁻¹) and scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radical (EC₅₀ of broad bean pod: 0.4 mg mL⁻¹; EC₅₀ of pea pod: 16.0 mg mL⁻¹) were also measured.
  
  
  CONCLUSIONS
  Broad bean and pea by-products are very rich in dietary fibre, particularly insoluble dietary fibre and their extractable polyphenols demonstrate antioxidant activity. Therefore they might be regarded as functional ingredients.
authors.affiliation: Dpto. Nutrición y Bromatología II: Bromatología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain. inmateos@farm.ucm.es.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Mateos-Aparicio
authors.forename: Araceli
authors.initials: A
authors.lastname: Redondo-Cuenca
authors.forename: María-José
authors.initials: MJ
authors.lastname: Villanueva-Suárez
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification; D004040:Dietary Carbohydrates / Q000032:analysis / Q000191:economics / Q000378:metabolism; D004043:Dietary Fiber / Q000032:analysis* / Q000191:economics / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry*; D005527:Food, Fortified / Q000191:economics; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis* / Q000191:economics; D008031:Lignin / Q000032:analysis / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism; D018532:Pisum sativum / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis / Q000191:economics / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D013030:Spain; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D007220:Industrial Waste; D059808:Polyphenols; D008031:Lignin; D000516:alpha-Amylases; C582722:termamyl; D005087:Glucan 1,4-alpha-Glucosidase