title: Lactobacillus species shift in distal esophagus of high-fat-diet-fed rats.
abstract: OBJECTIVE
  To analyze the microbiota shift in the distal esophagus of Sprague-Dawley rats fed a high-fat diet.
  
  
  METHODS
  Twenty Sprague-Dawley rats were divided into high-fat diet and normal control groups of 10 rats each. The composition of microbiota in the mucosa from the distal esophagus was analyzed based on selective culture. A variety of Lactobacillus species were identified by molecular biological techniques. Bacterial DNA from Lactobacillus colonies was extracted, and 16S rDNA was amplified by PCR using bacterial universal primers. The amplified 16S rDNA products were separated by denaturing gradient gel electrophoresis (DGGE). Every single band was purified from the gel and sent to be sequenced.
  
  
  RESULTS
  Based on mucosal bacterial culturing in the distal esophagus, Staphylococcus aureus was absent, and total anaerobes and Lactobacillus species were decreased significantly in the high-fat diet group compared with the normal control group (P < 0.01). Detailed DGGE analysis on the composition of Lactobacillus species in the distal esophagus revealed that Lactobacillus crispatus, Lactobacillus gasseri (L. gasseri) and Lactobacillus reuteri (L. reuteri) comprised the Lactobacillus species in the high-fat diet group, while the composition of Lactobacillus species in the normal control group consisted of L. gasseri, Lactobacillus jensenii and L. reuteri.
  
  
  CONCLUSIONS
  High-fat diet led to a mucosal microflora shift in the distal esophagus in rats, especially the composition of Lactobacillus species.
authors.affiliation: Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha, Hunan Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Cui
authors.forename: Jun-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Chen
authors.forename: Fang-Gen
authors.initials: FG
authors.lastname: Lu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001835:Body Weight; D015169:Colony Count, Microbial; D059305:Diet, High-Fat; D004947:Esophagus / Q000033:anatomy & histology / Q000382:microbiology*; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000254:growth & development*; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D051381:Rats; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S