title: Acquisition of neuron-like electrophysiological properties in neuroblastoma cells by controlled expression of NDM29 ncRNA.
abstract: Neuroblastoma is a pediatric cancer characterized by high malignancy and remarkable cell heterogeneity within the tumor nodules. It has been previously shown that the over-expression of a specific non-coding RNA, NDM29, reduces neuroblastoma development promoting cell differentiation. We have used neuroblastoma cells expressing NDM29 at its basal level (Mock cells) or at 5.4-fold higher levels (S1 cells) to investigate whether a functional differentiation correlates with morphological and biochemical development induced by NDM29 expression. First, analyzing the expression of specific markers we demonstrated that NDM29 expression is accompanied by a well coordinated differentiation process toward a neuron-like, rather than toward a glial-like, phenotype. Next, we defined the neuron-like traits of S1 in terms of secretion of cytokines involved in axon guidance, synapse formation and neurite outgrowth. Finally, we characterized the ionic channel apparatus of S1 cells by patch-clamp technique and compared with the Mock counterpart. S1 cells showed much higher levels of fast inactivating Na(+) current and were able to generate mature action potentials. Moreover, they developed expression of functional GABA(A) receptors on their membrane. In contrast, the two cell lines shared very similar pools of functional K(+) channels, although slight quantitative differences can be described. Our results suggest that a maturation occurs in neuroblastoma as a consequence of NDM29 expression, inducing the appearance of neuronal-like properties. In this context, S1 cells may represent a novel in vitro tool for electrophysiological and pharmacological studies of human cells of the neural lineage.
authors.affiliation: Institute of Biophysics, CNR, Genoa, Italy. gavazzo@ge.ibf.cnr.it
authors.forename: Paola
authors.initials: P
authors.lastname: Gavazzo
authors.forename: Serena
authors.initials: S
authors.lastname: Vella
authors.forename: Carla
authors.initials: C
authors.lastname: Marchetti
authors.forename: Mario
authors.initials: M
authors.lastname: Nizzari
authors.forename: Ranieri
authors.initials: R
authors.lastname: Cancedda
authors.forename: Aldo
authors.initials: A
authors.lastname: Pagano
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D022661:RNA, Untranslated / Q000235:genetics*
chemical_list: D015415:Biomarkers; D016207:Cytokines; D009419:Nerve Tissue Proteins; D022661:RNA, Untranslated