title: Lipidomics of polyunsaturated-fatty-acid-derived oxygenated metabolites.
abstract: Nutritionally important PUFAs (polyunsaturated fatty acids) mediate some of their bioactivities through formation of oxygenated metabolites. These bioactive lipids are formed by COX (cyclo-oxygenase), LOX (lipoxygenase) and cytochrome-P450-catalysed reactions, as well as non-enzymatic lipid peroxidation. These reactions produce numerous species, some of which can be formed through more than one pathway. MS-based lipidomics offers the selectivity and sensitivity required for qualitative and quantitative analysis of multiple lipid species, in a variety of biological systems, and can facilitate the study of these mediators.
authors.affiliation: Bradford School of Pharmacy and Centre for Skin Sciences, School of Life Sciences, University of Bradford, Bradford BD7 1DP, UK.
authors.forename: Karen A
authors.initials: KA
authors.lastname: Massey
authors.forename: Anna
authors.initials: A
authors.lastname: Nicolaou
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D008084:Lipoxygenase / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010100:Oxygen / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D003577:Cytochrome P-450 Enzyme System; D008084:Lipoxygenase; D010100:Oxygen