title: Studying RNA-protein interactions in vivo by RNA immunoprecipitation.
abstract: The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.
authors.affiliation: Dame Roma Mitchell Cancer Research Laboratories, The University of Adelaide, Adelaide, SA, Australia.
authors.forename: Luke A
authors.initials: LA
authors.lastname: Selth
authors.forename: Pierre
authors.initials: P
authors.lastname: Close
authors.forename: Jesper Q
authors.initials: JQ
authors.lastname: Svejstrup
mesh_terms: D002843:Chromatin / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D011485:Protein Binding; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology
chemical_list: D002843:Chromatin; D005656:Fungal Proteins; D012331:RNA, Fungal