title: Non-destructive measurement of demineralization & remineralization in the occlusal pits and fissures of extracted 3 molars with PS-OCT.
abstract: Previous studies have demonstrated that Polarization Sensitive Optical Coherence Tomography (PS-OCT) can be used to image the remineralization of early artificial caries lesion on smooth enamel surfaces of human and bovine teeth. However, most new dental decay is found in the pits and fissures of the occlusal surfaces of posterior dentition and it is in these high risk areas where the performance of new caries imaging devices need to be investigated. The purpose of this study was to demonstrate that PS-OCT can be used to measure the subsequent remineralization of artificial lesions produced in the pits and fissures of extracted 3(rd) molars. A PS-OCT system operating at 1310-nm was used to acquire polarization resolved images of occlusal surfaces exposed to a demineralizing solution at pH-4.5 followed by a fluoride containing remineralizing solution at pH-7.0 containing 2-ppm fluoride. The integrated reflectivity was calculated to a depth of 200-μm in the entire lesion area using an automated image processing algorithm. Although a well-defined surface zone was clearly resolved in only a few of the samples that underwent remineralization, the PS-OCT measurements indicated a significant (p<0.05) reduction in the integrated reflectivity between the severity of the lesions that were exposed to the remineralization solution and those that were not. The lesion depth and mineral loss were also measured with polarized light microscopy and transverse microradiography after sectioning the teeth. These results show that PS-OCT can be used to non-destructively monitor the remineralization potential of anti-caries agents in the important pits and fissures of the occlusal surface.
authors.affiliation: University of California, San Francisco, San Francisco, CA 94143-0758.
authors.forename: Chulsung
authors.initials: C
authors.lastname: Lee
authors.forename: Dennis J
authors.initials: DJ
authors.lastname: Hsu
authors.forename: Michael H
authors.initials: MH
authors.lastname: Le
authors.forename: Cynthia L
authors.initials: CL
authors.lastname: Darling
authors.forename: Daniel
authors.initials: D
authors.lastname: Fried