title: Mechanical shear contributes to granule formation resulting in quick start-up and stability of a hybrid anammox reactor.
abstract: It appears that if suspended biomass washout can be reduced effectively, granule formation will be fastened in fluidized bed. Quicker reactor start-up can be anticipated especially for those system keeping slow growth bacteria such as anammox. A hybrid reactor combined fixed-bed with nonwoven fabrics as biomass carrier and fluidized bed with slow speed mechanical stirring was therefore developed, and its nitrogen removal performances was evaluated experimentally. Only in 38 days, the total nitrogen removal rate (NRR) reached to 1.9 kg(N) m(-3) day (-1) and then doubled within 17 days, with total nitrogen removal efficiency kept above 70%. After 180 days reactor operating, the NRR reached a maximum value of 6.6 kg(N) m(-3) day(-1) and the specific anammox activity was gradually constant in 0.32 kg(N) kg(VSS)(-1) day(-1). Biomass attached on nonwoven fabrics could additionally improve reactor nitrogen removal by 8%. The dominant size of granular sludge reached to 0.78 mm with stirring speed adjusted from 30 to 80 rpm and the hydraulic retention time (HRT) from 8 to 1.5 h during the whole operating time. Scanning electron microscope observation showed especially compact structure of granular sludge. A 70% of anammox bacteria percentage was identified by fluorescence in situ hybridization analysis.
authors.affiliation: Faculty of Chemical, Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, 116023, China.
authors.forename: Yanning
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Liu
authors.forename: Fengxia
authors.initials: F
authors.lastname: Liu
authors.forename: Kenji
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001696:Biomechanical Phenomena; D019149:Bioreactors / Q000382:microbiology*; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D033081:Shear Strength
chemical_list: D000644:Quaternary Ammonium Compounds; D012722:Sewage; D009584:Nitrogen