title: Feasibility of treating irradiated bone with intramedullary delivered autologous mesenchymal stem cells.
abstract: BACKGROUND
  We aimed to explore (i) the short-term retention of intramedullary implanted mesenchymal stem cells BMSCs and (ii) their impact on the bone blood flow and metabolism in a rat model of hindlimb irradiation.
  
  
  METHODS
  Three months after 30 Gy irradiation, fourteen animals were referred into 2 groups: a sham-operated group (n = 6) and a treated group (n = 8) in which ¹¹¹In-labelled BMSCs (2 × 10⁶ cells) were injected in irradiated tibias. Bone blood flow and metabolism were assessed by serial (99m)Tc-HDP scintigraphy and 1-wk cell retention by recordings of (99m)Tc/¹¹¹In activities.
  
  
  RESULTS
  The amount of intramedullary implanted BMSCs was of 70% at 2 H, 40% at 48 H, and 38% at 168 H. Bone blood flow and bone metabolism were significantly increased during the first week after cell transplantation, but these effects were found to reduce at 2-mo followup. Conclusion. Short-term cell retention produced concomitant enhancement in irradiated bone blood flow and metabolism.
authors.affiliation: Head and Neck Surgery and Dental Units, Oncologic Surgery Department, Centre Alexis Vautrin, avenue de Bourgogne, Brabois, 54511 Vandoeuvre-lès-Nancy, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Phulpin
authors.forename: Gilles
authors.initials: G
authors.lastname: Dolivet
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Marie
authors.forename: Sylvain
authors.initials: S
authors.lastname: Poussier
authors.forename: Sandrine
authors.initials: S
authors.lastname: Huger
authors.forename: Pierre
authors.initials: P
authors.lastname: Bravetti
authors.forename: Pierre
authors.initials: P
authors.lastname: Graff
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Merlin
authors.forename: Nguyen
authors.initials: N
authors.lastname: Tran
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D005240:Feasibility Studies; D005720:Gamma Rays; D007204:Indium; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D010006:Osteoblasts / Q000737:chemistry / Q000378:metabolism; D011878:Radiotherapy; D051381:Rats; D017208:Rats, Wistar; D012039:Regional Blood Flow; D018709:Statistics, Nonparametric; D013977:Tibia / Q000098:blood supply / Q000378:metabolism / Q000528:radiation effects / Q000601:surgery*; D015899:Tomography, Emission-Computed, Single-Photon; D014182:Transplantation, Autologous
chemical_list: D015703:Antigens, CD; D007204:Indium