title: Correlation of HER 2/neu gene amplification with immunohistochemistry and other prognostic factors in breast carcinoma.
abstract: The purpose of this study was to determine relationship between HER-2/neu status and estrogen receptor, progesterone receptor, grade and age by comparing fluorescence in situ hybridization and immunohistochemistry. One hundred invasive breast carcinomas were reviewed and fluorescence in situ hybridization analysis was performed in all cases. Immunohistochemical scores showed a perfect concordance with fluorescence in situ hybridization amplification ratios (p < 0.0001). The results indicated a significant correlation between HER-2/neu and grade, but an inverse relationship between HER-2/neu and hormone receptors. In women aged ≤ 45 years, an inverse relationship between HER-2/neu and progesterone receptor was found and no association was noted between HER-2/neu and other factors. In women aged > 45 years, the results indicated a significant correlation between HER-2/neu and grade, and there was an inverse relationship between HER-2/neu and hormone receptors.
authors.affiliation: Department of Pathology, Anadolu Medical Center, Kocaeli, Turkey. saime.ramadan@anadolusaglik.org
authors.forename: Saime Sezgın
authors.initials: SS
authors.lastname: Ramadan
authors.forename: Ozlem
authors.initials: O
authors.lastname: Yapicier
authors.forename: Selçuk
authors.initials: S
authors.lastname: Kıhtır
authors.forename: Ayhan
authors.initials: A
authors.lastname: Erdemır
authors.forename: Tuğser Hakan
authors.initials: TH
authors.lastname: Doğan
authors.forename: Ismail
authors.initials: I
authors.lastname: Uskent
authors.forename: Haluk
authors.initials: H
authors.lastname: Onat
authors.forename: Metin
authors.initials: M
authors.lastname: Cakmakçi
mesh_terms: D000328:Adult; D000367:Age Factors; D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics; D002277:Carcinoma / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics; D016009:Chi-Square Distribution; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D016015:Logistic Models; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D016017:Odds Ratio; D011237:Predictive Value of Tests; D011379:Prognosis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D018570:Risk Assessment; D012307:Risk Factors; D014421:Turkey
chemical_list: D014408:Biomarkers, Tumor; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C508053:ERBB2 protein, human