title: Methylation-specific PCR.
abstract: DNA methylation patterns in CpG-rich regions of promoter, CpG islands, are concerned in regulation of gene expression in mammalian cells. Excessive methylation of CpG dinucleotides in promoter represses the gene expression. In cancer, especially, gene silencing is occurred through aberrant methylation in promoter of tumor suppressor genes. Methylation-specific PCR (MSP) is a method for analysis of DNA methylation patterns in CpG islands. For performing MSP, DNA is modified by and PCR performed with two primer pairs, which are detectable methylated and unmethylated DNA, respectively. MSP is a rapid measure for assession of the methylation status in CpG island.
authors.affiliation: Laboratory of Cell Biology, Korean Cell Line Bank, Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea. kujalok@snu.ac.kr
authors.forename: Ja-Lok
authors.initials: JL
authors.lastname: Ku
authors.forename: You-Kyung
authors.initials: YK
authors.lastname: Jeon
authors.forename: Jae-Gahb
authors.initials: JG
authors.lastname: Park
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D018899:CpG Islands / Q000235:genetics; D015925:Cryopreservation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D019175:DNA Methylation / Q000187:drug effects / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D013447:Sulfites / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D013447:Sulfites; D004247:DNA; C009279:sodium bisulfite