title: Analysis of vesicular trafficking in primary neurons by live imaging.
abstract: Alzheimer's disease, the most common neurodegenerative disease, is characterized by a progressive loss of synapses and accumulation of amyloid-beta (Aβ) peptides in the brain. Previous studies demonstrated that acute increase in synaptic activity in cultured hippocampal slices and mouse brains (Cirrito et al. Neuron 48: 913-922, 2005; Kamenetz et al. Neuron 37: 925-937, 2003) enhanced secretion of Aβ. Since synaptic activity promotes Aβ secretion, it could also affect the trafficking and processing of its precursor, the amyloid precursor protein (APP). Here, we describe a method to investigate the effect of acute synaptic activation on APP trafficking within dendrites.
authors.affiliation: Experimental Dementia Research Unit, Department of Experimental Medical Science, Wallenberg Neuroscience Center, Lund University, Lund, Sweden.
authors.forename: Davide
authors.initials: D
authors.lastname: Tampellini
authors.forename: Gunnar K
authors.initials: GK
authors.lastname: Gouras
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002470:Cell Survival; D006801:Humans; D007734:Kymography; D008055:Lipids; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D021381:Protein Transport; D013569:Synapses / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism*; D014162:Transfection
chemical_list: D016564:Amyloid beta-Protein Precursor; D008055:Lipids; C086724:Lipofectamine