title: Enterovirus 71 adsorption on metal ion-composite chitosan beads.
abstract: In this study, we developed composite chitosan beads combining various metal ions, including Ni(2+), Cu(2+), Zn(2+), and Fe(2+), for direct adsorption of enterovirus 71 (EV71). The metal-ion species had significant effects on the adsorption capacity of beads. Among these metal ion-composite chitosan beads, Ni(2+)-chitosan beads exhibited the best adsorption capacity of EV71. Using a concentration of 0.01-M Ni(2+) was found to best provide for bead formation and EV71 adsorption. The adsorption of EV71 for Ni(2+)-chitosan beads at neutral or alkaline pH was favored. Under a competitive condition with albumin proteins, Ni(2+)-chitosan beads exhibited significant capacity of EV71 adsorption in culture media. The adsorption of EV71 on the Ni(2+)-chitosan beads was attributed to the strong binding between Ni(2+) ions chelated to the surface amino acid of EV71 capsids and Ni(2+) ions chelated on the chitosan materials. Moreover, the adsorbed EV71 retained its antigenicity and infectivity after desorption. The Ni(2+)-chitosan beads exhibit a promising application to EV71 adsorption and removal.
authors.affiliation: Department of Biotechnology, Fooyin University, Kaohsiung City, Taiwan, Republic of [corrected] China. dr005@mail.fy.edu.tw
authors.forename: Ya-Ching
authors.initials: YC
authors.lastname: Lin
authors.forename: Shu-Ting
authors.initials: ST
authors.lastname: Lin
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Chen
authors.forename: Sheng-Chi
authors.initials: SC
authors.lastname: Wu
mesh_terms: D000327:Adsorption; D002614:Chelating Agents; D048271:Chitosan / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D029821:Enterovirus A, Human / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D007700:Kinetics; D008670:Metals / Q000378:metabolism*; D009532:Nickel / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D008670:Metals; D003300:Copper; D009532:Nickel; D048271:Chitosan; D007501:Iron; D015032:Zinc