title: Maternal microchimerism in pediatric inflammatory bowel disease.
abstract: Inflammatory bowel disease (IBD) shares many immunologic and clinical characteristics with graft versus host disease caused by allogeneic T lymphocytes after hematopoietic cell transplantation. Since maternal cells are known to enter the fetal circulation in a high proportion of pregnancies, we hypothesized that maternal engraftment in the fetus results in immune sequelae that can lead to IBD.
  
  
  METHODS
  The presence and extent of maternal microchimerism in tissues and blood samples from patients with Crohn's, Ulcerative colitis (UC), and control groups were determined using kinetic Polymerase Chain Reaction (kPCR) to detect maternal- and patient-specific HLA types. In addition, fluorescent in situ hybridization (FISH) was employed to detect maternal cells in biopsies from patients with IBD.
  
  
  RESULTS
  Using kPCR, maternal microchimerism was observed in 9 of the 16 (56%) patients with IBD and 6 out of 15 of the control group (40%) (P=NS). Five of 10 Crohn's patients had evidence of maternal microchimerism (50%) (P=NS). Four of six UC patients had evidence of maternal microchimerism in gut tissues (67%) (P=NS). There was no correlation between maternal michrochimerism and disease activity, disease location or granulomas in patients with IBD. Using FISH, five male Crohn's and five male UC patient's intestinal biopsies were analyzed for maternal microchimerism. No maternal cells were identified.
  
  
  CONCLUSIONS
  There is nothing in the data to suggest that patients with IBD differ from disease controls in their frequency of maternal microchimerism in either blood or gut mucosal tissues. These data suggest that maternal microchimerism in blood and biopsies is a relatively common phenomenon that has neither positive nor negative impact on IBD.
authors.affiliation: Department of Pediatrics; Seattle Children's Hospital; University of Washington; Seattle, WA USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Suskind
authors.forename: Denice
authors.initials: D
authors.lastname: Kong
authors.forename: Anne
authors.initials: A
authors.lastname: Stevens
authors.forename: Ghassan
authors.initials: G
authors.lastname: Wahbeh
authors.forename: Denise
authors.initials: D
authors.lastname: Christie
authors.forename: Lee-Ann
authors.initials: LA
authors.lastname: Baxter-Lowe
authors.forename: Marcus O
authors.initials: MO
authors.lastname: Muench