title: [Effect of trichostatin alone and combination with imatinb on the proliferation and apoptosis of the K562R cells].
abstract: OBJECTIVE
  To explore the effect of trichostatin A (TSA) alone and combination with imatinib on the proliferation and apoptosis of K562R cells.
  
  
  METHODS
  3-(4,5-dimethylthiazol-2-yl) -5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-V/propidium iodide(PI) staining.
  
  
  RESULTS
  After exposure to TSA, the proliferation of K562R cells was inhibited, and the effect was in both time- and dose-dependent manner. The apoptosis was induced. Combined with imatinib, the effect of proliferation inhibition was better.
  
  
  CONCLUSIONS
  TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis. TSA may become a new drug for imatinib-resistant patients.
authors.affiliation: Department of Hematology, Central South University, Changsha 410008, China. shuping1207@126.com
authors.forename: Shuping
authors.initials: S
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.forename: Qun
authors.initials: Q
authors.lastname: He
authors.forename: Xielan
authors.initials: X
authors.lastname: Zhao
authors.forename: Fangping
authors.initials: F
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001549:Benzamides; D049109:Cell Proliferation / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D000068877:Imatinib Mesylate; D020014:K562 Cells; D010879:Piperazines / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011743:Pyrimidines / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001549:Benzamides; D006877:Hydroxamic Acids; D010879:Piperazines; D011743:Pyrimidines; C012589:trichostatin A; D000068877:Imatinib Mesylate; D011505:Protein-Tyrosine Kinases