title: Aqueous extract from Vitis vinifera tendrils is able to enrich keratinocyte antioxidant defences.
abstract: An aqueous extract of V. vinifera L. tendrils was evaluated for its ability to enrich the antioxidant capacity of cultured cells. The long-time antioxidant capability of the extract was measured by in vitro chemical methods, and its influence on reduced glutathione levels and plasma membrane oxido reductase activity was determined in cultured human keratinocytes (NCTC 2544). Keratinocytes are cells normally exposed to oxidative stress, and for this reason adequately equipped with antioxidant defences. However, it has long been suggested that exogenous antioxidants may play an important role in minimizing the adverse effects of oxidative stress on skin.We demonstrated that V. vinifera tendril aqueous extract was able to increase, in a time- and dose-dependent manner, the reduced glutathione concentration and activity of trans plasma membrane oxido reductase as an indirect evaluation of the intracellular redox status of the cells demonstrating a relevant antioxidant activity of this phytocomplex.
authors.affiliation: Dipartimento di Scienze della Terra, della Vitae dell'Ambiente, Università degli Studi di Urbino "Carlo Bo", Urbino, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Fraternale
authors.forename: Roberta
authors.initials: R
authors.lastname: De Bellis
authors.forename: Cinzia
authors.initials: C
authors.lastname: Calcabrini
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Luigi
authors.initials: L
authors.lastname: Cucchiarini
authors.forename: Umberto
authors.initials: U
authors.lastname: Mancini
authors.forename: Marina
authors.initials: M
authors.lastname: Dachà
authors.forename: Donata
authors.initials: D
authors.lastname: Ricci
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D005419:Flavonoids / Q000737:chemistry; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D059808:Polyphenols; D027843:Vitis / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014867:Water