title: Comparative assessment of genetic variability in the populations of endemic and endangered yellow catfish, Horabagrus brachysoma (Teleostei: Horabagridae), based on allozyme, RAPD, and microsatellite markers.
abstract: The comparative assessment of genetic diversity using allozymes, random amplified polymorphic DNA (RAPD), and microsatellite markers was conducted in endemic and endangered yellow catfish (Horabagrus brachysoma) sampled from three locations in Western Ghats river systems of India. Among the three markers, microsatellites show more polymorphism, having 100% polymorphic loci, whereas allozymes show the least (56%). In RAPD, 60.5% of fragments were polymorphic. Observed heterozygosity and F(ST) values were very high in microsatellites, compared with the other markers. Microsatellite and RAPD markers reported a higher degree of genetic differentiation than allozymes among the populations depicted by pairwise F(ST)/G(ST), AMOVA, Nei's genetic distance, and UPGMA dendrogram. The three classes of markers demonstrated striking genetic differentiation between pairs of H. brachysoma populations. The data emphasize the need for fishery management, conservation, and rehabilitation of this species.
authors.affiliation: National Bureau of Fish Genetic Resources Cochin Unit, CMFRI Campus, Kerala, India. pmamuneer@gmail.com
authors.forename: P M A
authors.initials: PMA
authors.lastname: Muneer
authors.forename: A
authors.initials: A
authors.lastname: Gopalakrishnan
authors.forename: K K
authors.initials: KK
authors.lastname: Musammilu
authors.forename: V S
authors.initials: VS
authors.lastname: Basheer
authors.forename: V
authors.initials: V
authors.lastname: Mohindra
authors.forename: K K
authors.initials: KK
authors.lastname: Lal
authors.forename: K G
authors.initials: KG
authors.lastname: Padmakumar
authors.forename: A G
authors.initials: AG
authors.lastname: Ponniah
mesh_terms: D000483:Alleles; D000818:Animals; D002397:Catfishes / Q000235:genetics*; D056727:Endangered Species; D029941:Fish Proteins / Q000235:genetics*; D056426:Genetic Loci; D014644:Genetic Variation; D006579:Heterozygote; D007527:Isoenzymes / Q000235:genetics; D018895:Microsatellite Repeats; D010802:Phylogeny; D011153:Population / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D029941:Fish Proteins; D007527:Isoenzymes