title: Novel water-soluble red-emitting poly(p-phenylenevinylene) derivative: synthesis, characterization, and fluorescent acetylcholinesterase assays.
abstract: A new cyano-substituted poly(p-phenylenevinylene) (PPV) derivative, MEOPS-CNPPV, is synthesized through Knoevenagel condensation of anionic diacetonitrile and neutral dialdehyde and characterized by (1)H NMR, IR, elemental analysis, and gel-permeation chromatography (GPC). To our knowledge, the polymer is the first water-soluble red-emitting PPV derivative. The absorption and emission wavelength of this water-soluble conjugated polymer (CP) depend on the solvent. In buffer solution, the fluorescence of MEOPS-CNPPV is quenched by cationic dinitrobenzene derivatives. Further research indicates that dinitrobenzene derivative with a more flexible structure exhibits a larger K(sv). Making use of the charge reversal of dinitrobenzene-modified substrate, a "turn-on" method is developed for AChE activity assay with the new polymer as a fluorophore. This convenient and direct fluorometric assay thus provides a platform for novel red-emitting sensory systems.
authors.affiliation: Department of Chemistry, Hubei Key Laboratory on Organic and Polymeric Optoelectronic Materials, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Linna
authors.initials: L
authors.lastname: Zhu
authors.forename: Jingui
authors.initials: J
authors.lastname: Qin
authors.forename: Chuluo
authors.initials: C
authors.lastname: Yang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry*
chemical_list: D011145:Polyvinyls; D012997:Solvents; C470024:poly(4-phenylenevinylene); D014867:Water; D000110:Acetylcholinesterase