title: A new four-way variant t(5;17;15;20)(q33;q12;q22;q11.2) in acute promyelocytic leukemia.
abstract: Acute promyelocytic leukemia (APL) is characterized by t(15;17)(q22;q21), which results in the fusion of the promyelocytic leukemia (PML) gene at 15q22 with the retinoic acid alpha-receptor (RARA) at 17q21. We report a patient with APL carrying a new complex variant translocation (5;17;15;20). Spectral karyotyping analysis of bone marrow cells revealed t(5;17;15;20)(q33;q12;q22;q11.2). Fluorescence in situ hybridization with a PML/RARA dual-color DNA probe showed a single fusion signal, and RT-PCR analysis showed PML/RARA fusion transcripts. Complete remission was attained with a course of conventional chemotherapy with all-trans retinoic acid (ATRA). To our knowledge, this is the first report of a four-way translocation of 5q33 and 20q11 involvement in APL.
authors.affiliation: Departments of Bioregulatory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan. yamanouc@m.ehime-u.ac.jp.
authors.forename: Jun
authors.initials: J
authors.lastname: Yamanouchi
authors.affiliation: Blood Transfusion and Cell Therapy, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hato
authors.affiliation: Laboratory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Niiya
authors.affiliation: Departments of Bioregulatory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Miyoshi
authors.affiliation: Departments of Bioregulatory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Taichi
authors.initials: T
authors.lastname: Azuma
authors.affiliation: Departments of Bioregulatory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Ikuya
authors.initials: I
authors.lastname: Sakai
authors.affiliation: Departments of Bioregulatory Medicine, Ehime University Graduate School of Medicine, 454 Shitsukawa, Toon, Ehime, 7910295, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Yasukawa
mesh_terms: D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002884:Chromosomes, Human, Pair 15; D002886:Chromosomes, Human, Pair 17; D002890:Chromosomes, Human, Pair 20; D002895:Chromosomes, Human, Pair 5; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014178:Translocation, Genetic; D014212:Tretinoin / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D014212:Tretinoin