title: The evaluation of hypoxia-inducible factor 1 in N-nitro-L-arginine methyl ester preeclampsia model of pregnant rats.
abstract: OBJECTIVE
  The objective of the study was to evaluate hypoxia-inducible factor 1 (HIF-1), which plays a major role in the stimulation of angiogenesis in placental tissues, by using immunohistochemical staining in preeclampsia model of rats, developed by N-nitro-L-arginine methyl ester (L-NAME) METHODS: Thirty pregnant rats were randomized into 2 groups (n = 15 in each group) on day 10 of gestation. L-NAME was given to rats in the study group by gavage. On days 0, 10, and 20 of gestation, rats were weighted, and urine protein values and blood pressures were measured. Hypoxia-inducible factor 1 expressions were assessed with immunohistochemical staining by using avidin-biotin peroxidase via selecting preparation.
  
  
  RESULTS
  Systolic and diastolic blood pressures and urine protein value of L-NAME group on day 20 of gestation were found to be significantly higher than those obtained on days 0 and 10 of gestation in the same group and those obtained on day 20 of gestation in the sham group (P < 0.05). Maternal weight, number of fetuses, and mean fetal weight of rats in L-NAME group on day 20 of gestation were found to be significantly lower than those obtained from rats in the sham group (P < 0.05). Regarding HIF-1 expression of placental tissues, mild immunohistochemical staining was found in 2 rats (13.4%) and moderate in 13 rats (86.6%) in the L-NAME group. A significant difference was found in terms of HIF-1 positivity in the maternal placentas of both groups (P < 0.05).
  
  
  CONCLUSIONS
  L-NAME preeclampsia model of pregnant rats is consistent with human preeclampsia in terms of hypertension, proteinuria, and intrauterine growth retardation; in addition, it also shows evidence of placental hypoxia findings.
authors.affiliation: Department of Pediatrics, Cumhuriyet University School of Medicine, Sivas, Turkey. dralikaya@gmail.com
authors.forename: Ali
authors.initials: A
authors.lastname: Kaya
authors.forename: Abdullah
authors.initials: A
authors.lastname: Boztosun
authors.forename: Hulya
authors.initials: H
authors.lastname: Seckin
authors.forename: Ahmet Sami
authors.initials: AS
authors.lastname: Guven
authors.forename: Zekeriya
authors.initials: Z
authors.lastname: Kucukdurmaz
authors.forename: Sefa
authors.initials: S
authors.lastname: Gulturk
authors.forename: Omer
authors.initials: O
authors.lastname: Cevit
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D003971:Diastole / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus / Q000378:metabolism; D006801:Humans; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D011247:Pregnancy; D011507:Proteinuria / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013599:Systole / Q000502:physiology; D014327:Trophoblasts / Q000378:metabolism / Q000473:pathology
chemical_list: D051793:Hypoxia-Inducible Factor 1; D019331:NG-Nitroarginine Methyl Ester