title: Detection of clinically important β-lactamases in commensal Escherichia coli of human and swine origin in western China.
abstract: Data correlating β-lactamases found in commensal Escherichia coli of human and animal origin are limited. In this study, 447 commensal E. coli isolates from the faeces of humans and swine (280 human isolates from four hospitals and 167 swine isolates from seven farms) were collected between September 2006 and January 2009 in western China. For extended-spectrum β-lactamase (ESBL)-producing and other cephalosporin-resistant isolates, the relevant β-lactamase genes (bla(TEM), bla(SHV), bla(CTX-M-1/2/9) group, bla(CMY-2) and bla(KPC)) were detected by PCR analysis. Of the 447 isolates tested, 120 (26.8 %) were confirmed as producing ESBL. Among these, 70 and 40 human isolates carried a member of the bla(CTX-M-1 )group (13 bla(CTX-M-3), 21 bla(CTX-M-15), four bla(CTX-M-22), eight bla(CTX-M-28), four bla(CTX-M-36), 15 bla(CTX-M-55) and five bla(CTX-M-69)) or bla(SHV) (14 bla(SHV-2), seven bla(SHV-5), ten bla(SHV-12), five bla(SHV-57) and four bla(SHV-97)),respectively, whilst six and four swine isolates carried a member of the bla(CTX-M-1 )group (one bla(CTX-M-15) and five bla(CTX-M-22)) or bla(SHV) (three bla(SHV-2) and one bla(SHV-12)), respectively. Furthermore, 59 human and swine isolates and seven human isolates carried bla(CMY-2) and bla(KPC), respectively. These findings indicate that the bla(CTX-M-1) group, including the novel variant bla(CTX-M-69), and bla(SHV) are the predominant ESBL genes in both humans and swine in western China, and bla(CMY-2) is also common in both groups. The carriage rates of broad-spectrum β-lactamases among commensal E. coli was much lower in swine than in humans, suggesting that β-lactamase genes have not established themselves in animal ecosystems in western China.
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Guo-Bao
authors.initials: GB
authors.lastname: Tian
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Hong-Ning
authors.initials: HN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: An-Yun
authors.initials: AY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Wen-Qiao
authors.initials: WQ
authors.lastname: Fan
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Xu
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Zhong-Bin
authors.initials: ZB
authors.lastname: Guan
authors.affiliation: Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, Chengdu, Sichuan, PR China.|'985 Project' Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan, PR China.|School of Life Science, Sichuan University, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Sichuan, PR China.
authors.forename: Li-Kou
authors.initials: LK
authors.lastname: Zou
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases