title: Bone morphogenetic protein-7 reduces cold ischemic injury in rat kidney.
abstract: BACKGROUND
  Deceased donor kidneys have a high incidence of delayed graft function attributable to ischemia-reperfusion injury. Although preservation solution and cold storage reduce cold ischemic injury, the depletion of cellular energy and oxidative signalling leads to cellular damage. Because bone morphogenetic protein-7 has renoprotective effect, we have hypothesized that recombinant human bone morphogenetic protein-7 (rh BMP-7) will better preserve kidney tissue exposed to prolonged cold ischemia in comparison with University of Wisconsin (UW) solution. We evaluated how the duration of cold ischemia influences the cold ischemic injury.
  
  
  METHODS
  Levels of lipid peroxidation, protein carbonyl content, as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were determined in the kidneys by spectrophotometry after 6, 12, and 24 hours of cold ischemia.
  
  
  RESULTS
  Time-dependent increases in the levels of lipid peroxidation and protein carbonyl content at all time points were significantly lower in rhBMP-7-perfused kidneys versus the UW-treated group. SOD activity after 6 hours, as well as GSH-Px activity after 24 hours of cold ischemia was significantly higher in the kidney tissue perfused by rhBMP-7 than in UW group.
  
  
  CONCLUSIONS
  rhBMP-7 significantly decreases cellular damage in rat kidney versus UW preservation solution and this is attributed to lowering of cold ischemia injury and maintaining prolonged tissue antioxidant activity.
authors.affiliation: Department of Anatomy, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
authors.forename: T
authors.initials: T
authors.lastname: Celic
authors.forename: J
authors.initials: J
authors.lastname: Spanjol
authors.forename: A
authors.initials: A
authors.lastname: Grskovic
authors.forename: D
authors.initials: D
authors.lastname: Markic
authors.forename: I
authors.initials: I
authors.lastname: Prebilic
authors.forename: Z
authors.initials: Z
authors.lastname: Fuckar
authors.forename: D
authors.initials: D
authors.lastname: Bobinac
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000627:therapeutic use; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D055419:Bone Morphogenetic Protein 7; D011994:Recombinant Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase