title: Rapid detection of Bordetella pertussis and Bordetella parapertussis in clinical and molecular proficiency panel specimens with a novel intercalating dye-based PCR assay.
abstract: A novel rapid internally-controlled duplex polymerase chain reaction (PCR) assay for Bordetella pertussis and Bordetella parapertussis that uses a new intercalating dye, LCGreen, for amplicon detection was designed and evaluated using clinical specimens and the 2009 Quality Control for Molecular Diagnostics (QCMD) molecular proficiency testing panel. The sensitivity and specificity of the new PCR assay were equal to that of reference standard uniplex probe-based assays. The assay can be performed in 1 h including DNA extraction. The new assay thus offers a simple and rapid alternative for the detection of B. pertussis and B. parapertussis.
authors.affiliation: Children's Hospital of Eastern Ontario, University of Ottawa, Ottawa, Ontario, Canada. slinger@cheo.on.ca
authors.forename: Robert
authors.initials: R
authors.lastname: Slinger
authors.forename: Ioana
authors.initials: I
authors.lastname: Moldovan
authors.forename: Lucie
authors.initials: L
authors.lastname: Hyde
authors.forename: Francis
authors.initials: F
authors.lastname: Chan
mesh_terms: D001885:Bordetella Infections / Q000175:diagnosis* / Q000382:microbiology; D042483:Bordetella parapertussis / Q000235:genetics / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007364:Intercalating Agents / Q000378:metabolism; D025202:Molecular Diagnostic Techniques / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards; D013997:Time Factors
chemical_list: D007364:Intercalating Agents