title: Byssus thread: a novel support material for urease immobilization.
abstract: Byssus threads are tough biopolymer produced by mussels (Mytilus viridis) to attach themselves to rocks. These were collected from mussels in their natural habitat (N) and from animals maintained in laboratory condition (L) as a novel support. Byssus thread surfaces were characterized by SEM analysis, chemically modified and used for adsorption of urease. The efficiency of the immobilization was calculated by examining the relative enzyme activity of free and the immobilized urease. The pH stabilities of immobilized urease were higher (0.5 unit) than free enzyme. Immobilized enzymes on byssus (both N and L) when stored at 6 °C retained 50% of its activity after 30 days, but they were more stable in dry condition. The optimum temperature of immobilized enzymes was found to increase (25 °C). A Michaelis-Menten constant (K (m)) value for immobilized urease was also elevated (2.08 mol).
authors.affiliation: Department of Zoology, Sir P. T. Sarvajanik College of Science, Surat 395001, India. neelam1735@gmail.com
authors.forename: Neelam
authors.initials: N
authors.lastname: Mishra
authors.forename: Kespi
authors.initials: K
authors.lastname: Pithawala
authors.forename: Anita
authors.initials: A
authors.lastname: Bahadur
mesh_terms: D000327:Adsorption; D000818:Animals; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism; D001709:Biotechnology; D049872:Bivalvia / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007887:Fabaceae / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D014510:Urease / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D004800:Enzymes, Immobilized; D010940:Plant Proteins; D014510:Urease