title: The effects of the broad-specificity lipase inhibitor, tetrahydrolipstatin, on the growth, development and survival of the larvae of Epiphyas postvittana (Walker) (Tortricidae, Lepidoptera).
abstract: The effects of the lipase inhibitor, tetrahydrolipstatin (THL), on neonate Epiphyas postvittana (Walker) (Lepidoptera, Tortricidae) larvae were investigated by feeding on control artificial diets (with and without 2% ethanol) and diets containing 2% ethanol and one of three concentrations of THL (0.011%, 0.037% and 0.11%). Small but significant reductions in growth rate, percent pupation and time to pupation were observed for larvae feeding on 2% ethanol control diet compared with standard control diet, but larger reductions in all parameters occurred with increasing THL concentration. Third instar larvae fed 0.011% THL in the diet had 40% of the midgut lipase activity in the relevant control larvae and showed up-regulation of gene expression of the gastric lipase-like family but not the pancreatic lipase-like family of midgut lipases.
authors.affiliation: The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
authors.forename: Ngaire P
authors.initials: NP
authors.lastname: Markwick
authors.forename: Joanne
authors.initials: J
authors.lastname: Poulton
authors.forename: Tony K
authors.initials: TK
authors.lastname: McGhie
authors.forename: Mark W
authors.initials: MW
authors.lastname: Wohlers
authors.forename: John T
authors.initials: JT
authors.lastname: Christeller
mesh_terms: D000818:Animals; D004032:Diet; D005260:Female; D041981:Gastrointestinal Tract / Q000201:enzymology; D015870:Gene Expression; D007783:Lactones / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008297:Male; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development*; D000077403:Orlistat; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D007783:Lactones; D012333:RNA, Messenger; D000077403:Orlistat; D008049:Lipase