title: Prior endurance exercise prevents postprandial lipaemia-induced increases in reactive oxygen species in circulating CD31+ cells.
abstract: We hypothesized that prior exercise would prevent postprandial lipaemia (PPL)-induced increases in intracellular reactive oxygen species (ROS) in three distinct circulating angiogenic cell (CAC) subpopulations. CD34(+), CD31(+)/CD14(-)/CD34(-), and CD31(+)/CD14(+)/CD34(-) CACs were isolated from blood samples obtained from 10 healthy men before and 4 h after ingesting a high fat meal with or without ∼50 min of prior endurance exercise. Significant PPL-induced increases in ROS production in both sets of CD31(+) cells were abolished by prior exercise. Experimental ex vivo inhibition of NADPH oxidase activity and mitochondrial ROS production indicated that mitochondria were the primary source of PPL-induced oxidative stress. The attenuated increases in ROS with prior exercise were associated with increased antioxidant gene expression in CD31(+)/CD14(-)/CD34(-) cells and reduced intracellular lipid uptake in CD31(+)/CD14(+)/CD34(-) cells. These findings were associated with systemic cardiovascular benefits of exercise, as serum triglyceride, oxidized low density lipoprotein-cholesterol, and plasma endothelial microparticle concentrations were lower in the prior exercise trial than the control trial. In conclusion, prior exercise completely prevents PPL-induced increases in ROS in CD31(+)/CD14(-)/CD34(-) and CD31(+)/CD14(+)/CD34(-) cells. The mechanisms underlying the effects of exercise on CAC function appear to vary among specific CAC types.
authors.affiliation: Department of Kinesiology, School of Public Health, University of Maryland College Park, College Park, MD 20742-2611, USA.
authors.forename: Nathan T
authors.initials: NT
authors.lastname: Jenkins
authors.forename: Rian Q
authors.initials: RQ
authors.lastname: Landers
authors.forename: Sunny R
authors.initials: SR
authors.lastname: Thakkar
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Fan
authors.forename: Michael D
authors.initials: MD
authors.lastname: Brown
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Prior
authors.forename: Espen E
authors.initials: EE
authors.lastname: Spangenburg
authors.forename: James M
authors.initials: JM
authors.lastname: Hagberg
mesh_terms: D000293:Adolescent; D000328:Adult; D055252:Cell-Derived Microparticles; D059305:Diet, High-Fat; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015444:Exercise; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress; D010807:Physical Endurance; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism*; D019518:Postprandial Period / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
chemical_list: D004791:Enzyme Inhibitors; D008077:Lipoproteins, LDL; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D017382:Reactive Oxygen Species; D014280:Triglycerides; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D019255:NADPH Oxidases