title: Promising new potential for mesenchymal stem cells derived from human umbilical cord Wharton's jelly: sweat gland cell-like differentiative capacity.
abstract: Mesenchymal stem cells derived from Wharton's jelly of the human umbilical cord (hUC-MSCs) possess various advantageous properties, similar to bone marrow-derived mesenchymal stem cells (BM-MSCs), including self-renewal, extended proliferation potential and multilineage differentiation potential. In this study, we hoped to determine whether hUC-MSCs could be induced to differentiate into sweat gland cell-like cells, that would be potential in sweat glands restoration after injury. In this study, the results of flow cytometry analysis revealed that hUC-MSCs showed the typical antigen profile of MSCs and were positive for CD29, CD44, CD90, CD105 and Oct-4; they were negative for the antigens of CD34, CEA and CK14. Remarkably, hUC-MSCs maintained proper proliferation and differentiation ability. After culture in sweat gland cell-conditioned medium (induction group 1) for 3 weeks, hUC-MSCs possessed sweat gland cell-like morphology and expressed markers of sweat gland cells (CEA, CK14 and CK19) more efficiently than those of induction group 2. In reverse-transcription PCR and western blotting analysis, it was further confirmed that induced hUC-MSCs (group 1) also expressed a higher level of sweat gland developmental genes (EDA and EDAR) than group 2. These results together provided evidence that hUC-MSCs could possess a new emerging potential to differentiate into sweat gland cell-like cells with a higher efficacy under our new induction system. Thus, hUC-MSCs could be considered a new strategy for sweat glands restoration after skin injury as well as improvement of cutaneous regeneration.
authors.affiliation: Wound Healing and Cell Biology Laboratory, Burns Institute, First Affiliated Hospital/General Hospital of PLA, Trauma Centre of Postgraduate Medical College, Beijing, People's Republic of China.
authors.forename: Yongan
authors.initials: Y
authors.lastname: Xu
authors.forename: Sha
authors.initials: S
authors.lastname: Huang
authors.forename: Kui
authors.initials: K
authors.lastname: Ma
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.forename: Weidong
authors.initials: W
authors.lastname: Han
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Sheng
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002478:Cells, Cultured; D060833:Cellular Microenvironment; D005260:Female; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D013545:Sweat Glands / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*; D059631:Wharton Jelly / Q000166:cytology*
chemical_list: D015415:Biomarkers