title: Moracenin D from Mori Cortex radicis protects SH-SY5Y cells against dopamine-induced cell death by regulating nurr1 and α-synuclein expression.
abstract: In our efforts to find neuroprotective materials of plant origin, several compounds were isolated from Mori Cortex Radicis. The protective effect against dopamine-induced cell death was examined, and the subsequent effects on the levels of expression of Parkinson's disease-associated nurr1 and α-synuclein were evaluated in a dopamine-induced system. Five compounds were isolated and moracenin D protected cell death against dopamine-induction in human neuroblastoma SH-SY5Y cells. The effects of moracenin D on the levels of mRNA and protein expression of nurr1 and α-synuclein were subsequently examined using reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. Treatment with moracenin D resulted in an up-regulation of nurr1 mRNA levels and a down-regulation of α-synuclein mRNA levels. Additionally, the α-synuclein protein expression was decreased in accordance with an increase in nurr1 protein expression. These results demonstrate that the protective effects of moracenin D were presumably due to the correlative effects on the up-regulation of nurr1 and down-regulation of α-synuclein expressions against dopamine induction. Therefore, moracenin D can be considered as a candidate for therapy for Parkinson's disease.
authors.affiliation: Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Korea.
authors.forename: Ahrom
authors.initials: A
authors.lastname: Ham
authors.forename: Hak Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Seong Su
authors.initials: SS
authors.lastname: Hong
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Woongchon
authors.initials: W
authors.lastname: Mar
mesh_terms: D001572:Benzofurans / Q000494:pharmacology; D015153:Blotting, Western; D016923:Cell Death; D045744:Cell Line, Tumor; D002470:Cell Survival; D004298:Dopamine / Q000009:adverse effects*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000432:Methanol / Q000737:chemistry; D029586:Moraceae / Q000737:chemistry; D009447:Neuroblastoma / Q000473:pathology; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2 / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D001572:Benzofurans; D005419:Flavonoids; C539882:NR4A2 protein, human; D018696:Neuroprotective Agents; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D010936:Plant Extracts; D012333:RNA, Messenger; D051844:alpha-Synuclein; C574050:moracenin D; D004298:Dopamine; D000432:Methanol