title: Intraepithelial infiltration by mast cells in human Helicobacter pylori active gastritis.
abstract: Recent observations suggest an involvement of mast cells in Helicobacter pylori gastritis, but the mechanism of intraepithelial mast cell activation in H. pylori-infected patients remains to be clarified. Intraepithelial mast cells, identified by immunohistochemistry for CD117, were quantified in antral biopsies from 6 patients with H. pylori "active" chronic gastritis, 7 patients with H. pylori "nonactive" gastritis, and 9 controls. Antral biopsies from patients with H. pylori "active" gastritis showed higher intraepithelial mast cell counts than those from patients with H. pylori "nonactive" gastritis and from controls. Electron microscopy, selectively performed in 6 cases of H. pylori "active" gastritis, confirmed the presence of intraepithelial mast cells and allowed their subdivision into mature cells with intact electron-dense granules or degranulated cells. Other mast cells appeared to migrate through defects in the basement membrane into the epithelial layer. Mast cells in these areas often showed piecemeal degranulation or were characterized by large canaliculi, expanded Golgi areas, and a few granules, a process similar to the phase of recovery from anaphylactic degranulation of isolated human mast cells. The possible significance of these unusual ultrastructural findings is discussed.
authors.affiliation: Department of Human Pathology, University of Messina, Messina, Italy. rosariocaruso@tin.it
authors.forename: Rosario Alberto
authors.initials: RA
authors.lastname: Caruso
authors.forename: Antonino
authors.initials: A
authors.lastname: Parisi
authors.forename: Costantino
authors.initials: C
authors.lastname: Crisafulli
authors.forename: Anna
authors.initials: A
authors.lastname: Bonanno
authors.forename: Roberta
authors.initials: R
authors.lastname: Lucian
authors.forename: Giovanni
authors.initials: G
authors.lastname: Branca
authors.forename: Marco
authors.initials: M
authors.lastname: Scardigno
authors.forename: Francesco
authors.initials: F
authors.lastname: Fedele
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D016022:Case-Control Studies; D015550:Cell Degranulation; D002465:Cell Movement; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004847:Epithelial Cells / Q000382:microbiology / Q000648:ultrastructure*; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology / Q000648:ultrastructure*; D005756:Gastritis / Q000382:microbiology / Q000473:pathology*; D016481:Helicobacter Infections / Q000382:microbiology / Q000473:pathology*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D007150:Immunohistochemistry; D007558:Italy; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis
chemical_list: D015415:Biomarkers; D019009:Proto-Oncogene Proteins c-kit