title: Suicide inactivation of peroxidase from Chamaerops excelsa palm tree leaves.
abstract: The concentration and time-dependences and the mechanism of the inactivation of Chamaerops excelsa peroxidase (CEP) by hydrogen peroxide were studied kinetically with four co-substrates (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), guaiacol, o-dianisidine and o-phenylenediamine). The turnover number (r) of H(2)O(2) required to complete the inactivation of the enzyme varied for the different substrates, the enzyme most resistant to inactivation (r=4844) with ABTS being the most useful substrate for biotechnological applications, opening a new avenue of enquiry with this peroxidase.
authors.affiliation: Departamento de Química Física, Facultad de Ciencias Químicas, Universidad de Salamanca, 37008 Salamanca, Spain.
authors.forename: Nazaret Hidalgo
authors.initials: NH
authors.lastname: Cuadrado
authors.forename: Galina G
authors.initials: GG
authors.lastname: Zhadan
authors.forename: Manuel G
authors.initials: MG
authors.lastname: Roig
authors.forename: Valery L
authors.initials: VL
authors.lastname: Shnyrov
mesh_terms: D028023:Arecaceae / Q000737:chemistry / Q000201:enzymology*; D052160:Benzothiazoles / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002845:Chromatography; D003962:Dianisidine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006139:Guaiacol / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D010655:Phenylenediamines / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology*; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification / Q000378:metabolism; D012996:Solutions; D013379:Substrate Specificity; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D052160:Benzothiazoles; D010655:Phenylenediamines; D010940:Plant Proteins; D012996:Solutions; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006139:Guaiacol; C034193:1,2-diaminobenzene; D006861:Hydrogen Peroxide; D009195:Peroxidase; D003962:Dianisidine