title: Prompt administration of Crimean-Congo hemorrhagic fever (CCHF) virus hyperimmunoglobulin in patients diagnosed with CCHF and viral load monitorization by reverse transcriptase-PCR.
abstract: Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the genus Nairovirus of the family Bunyaviridae, causes a severe disease in humans with high mortality rates. In Turkey, the number of patients with CCHF has increased since 2002. Here, we aimed to treat CCHF patients with CCHFV hyperimmunoglobulin. We prepared a CCHFV hyperimmunoglobulin product from 22 individuals who survived CCHF infection. A total of 26 CCHF patients were enrolled into this study. For CCHFV hyperimmunoglobulin administration, a Kubar Unit (KU) was defined. As a standard therapeutic approach, 400 KU of hyperimmunoglobulin were given to each patient as a single dose before viral load was detected. We used one-step real-time reverse transcriptase-PCR to monitor the viral load of CCHF patients. According to the one-step real-time PCR results, 15 patients with a viral load of 10(8) copies/mL or more were defined as high risk. In this high-risk group, the survival rate was found to be 86.6% (13/15) and 2 patients died despite CCHFV hyperimmunoglobulin administration. CCHF is a very serious and highly fatal infection, particularly for patients in the defined high-risk group. Prompt administration of CCHFV hyperimmunoglobulin might be a very promising new treatment approach, especially for high-risk individuals.
authors.affiliation: Gulhane Military School of Medicine, Ankara, Turkey. akubar＠hotmail.com
authors.forename: Ayhan
authors.initials: A
authors.lastname: Kubar
authors.forename: Mustafa
authors.initials: M
authors.lastname: Haciomeroglu
authors.forename: Aykut
authors.initials: A
authors.lastname: Ozkul
authors.forename: Umit
authors.initials: U
authors.lastname: Bagriacik
authors.forename: Esragul
authors.initials: E
authors.lastname: Akinci
authors.forename: Kenan
authors.initials: K
authors.lastname: Sener
authors.forename: Hurrem
authors.initials: H
authors.lastname: Bodur
mesh_terms: D000914:Antibodies, Viral / Q000008:administration & dosage*; D016903:Drug Monitoring / Q000379:methods*; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000276:immunology*; D006479:Hemorrhagic Fever, Crimean / Q000628:therapy*; D006801:Humans; D007116:Immunization, Passive / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D016019:Survival Analysis; D016896:Treatment Outcome; D014421:Turkey; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral