title: Regeneration of periodontal tissues in non-human primates with rhGDF-5 and beta-tricalcium phosphate.
abstract: The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.
authors.affiliation: Medtronic, Spinal & Biologics Division, Memphis, TN 38132, USA. kelly.b.emerton@medtronic.com
authors.forename: K B
authors.initials: KB
authors.lastname: Emerton
authors.forename: S J
authors.initials: SJ
authors.lastname: Drapeau
authors.forename: H
authors.initials: H
authors.lastname: Prasad
authors.forename: M
authors.initials: M
authors.lastname: Rohrer
authors.forename: P
authors.initials: P
authors.lastname: Roffe
authors.forename: K
authors.initials: K
authors.lastname: Hopper
authors.forename: J
authors.initials: J
authors.lastname: Schoolfield
authors.forename: A
authors.initials: A
authors.lastname: Jones
authors.forename: D L
authors.initials: DL
authors.lastname: Cochran
mesh_terms: D000539:Alveolar Process / Q000000981:diagnostic imaging / Q000254:growth & development; D000704:Analysis of Variance; D000818:Animals; D002130:Calcium Phosphates; D055113:Chronic Periodontitis / Q000188:drug therapy*; D003739:Dental Cementum / Q000000981:diagnostic imaging / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D055428:Growth Differentiation Factor 5 / Q000008:administration & dosage*; D006801:Humans; D010215:Papio; D010513:Periodontal Ligament / Q000000981:diagnostic imaging / Q000254:growth & development; D011897:Random Allocation; D011994:Recombinant Proteins / Q000008:administration & dosage; D012038:Regeneration; D055114:X-Ray Microtomography
chemical_list: D002130:Calcium Phosphates; D004337:Drug Carriers; D055428:Growth Differentiation Factor 5; D011994:Recombinant Proteins; C485817:beta-tricalcium phosphate