title: [The epidemiological features of visceral leishmaniasis, revealed on examination of children by polymerase chain reaction, in the Papsky District, Namangan Region, Uzbekistan].
abstract: Patients with visceral leishmaniasis (VL) have been registered in the Papsky District, Namangan Region, Uzbekistan, over the past 23 years. A total of 95 patients were notified in 1987 to 2009. In 2007-2008, a mass survey using the polymerase chain reaction (PCR) within the international INTAS project 05-100006-8043 was conducted in 5 population aggregates of the Papsky District, Namangan Region, Uzbekistan, where VL cases had been regularly registered in the last years. Bone marrow and venous and peripheral blood smears were used as a test material. A total of 234 samples, including 3 bone marrow biopsy specimens, 9 venous blood samples and 222 peripheral blood ones, were tested. All the samples were on the glass slides. Three groups were identified among the examinees. Group 1 consisted of 13 subjects who had been ill at different times. Group 2 comprised 27 children treated at hospital for various diagnoses. Group 3 (the largest one, n=190) included apparently healthy children. All the children of this group felt well and had no symptoms of any illnesses at the examination. In this group, 85 (44.7%) subjects were PCR-positive. Twenty-four (55.8%) of 43 children in the age group of 0-3 years were PCR-positive; the 4-7-year age group comprised 66 subjects and 33 (50%) of them were PCR-positive. Group over 7 years of age included 81 subjects; 45 (55.5%) were PCR-positive. The results of the mass survey with PCR, which covered the 5 population aggregates in the Papsky District, Namangan Region, Uzbekistan, suggest the epidemic activity of a synathropic focus of VL and make us look at many fixed notions of its epidemiology in new contexts.
authors.forename: E N
authors.initials: EN
authors.lastname: Zhirenkina
authors.forename: E N
authors.initials: EN
authors.lastname: Ponirovskiĭ
authors.forename: M V
authors.initials: MV
authors.lastname: Strelkova
authors.forename: E N
authors.initials: EN
authors.lastname: Morozov
authors.forename: P N
authors.initials: PN
authors.lastname: Flegontov
authors.forename: A A
authors.initials: AA
authors.lastname: Kolesnikov
authors.forename: V I
authors.initials: VI
authors.lastname: Ponomareva
authors.forename: R M
authors.initials: RM
authors.lastname: Nasyrova
authors.forename: D A
authors.initials: DA
authors.lastname: Kovalenko
authors.forename: A A
authors.initials: AA
authors.lastname: Fatullaeva
authors.forename: Sh A
authors.initials: ShA
authors.lastname: Razakov
authors.forename: L
authors.initials: L
authors.lastname: Shnur
authors.forename: G
authors.initials: G
authors.lastname: Banet
authors.forename: A
authors.initials: A
authors.lastname: Varburg
authors.forename: G
authors.initials: G
authors.lastname: Shonian
mesh_terms: D000293:Adolescent; D000818:Animals; D001853:Bone Marrow / Q000469:parasitology*; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000032:analysis; D005260:Female; D006801:Humans; D007223:Infant; D007303:Insect Vectors / Q000469:parasitology; D018314:Leishmania infantum / Q000502:physiology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology; D008137:Longitudinal Studies; D008297:Male; D010691:Phlebotomus / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012737:Sex Factors; D014610:Uzbekistan
chemical_list: D004247:DNA