title: Flavanone glycosides from viscum coloratum and their inhibitory effects on osteoclast formation.
abstract: Two novel flavanone glycosides, homoeriodictyol 7-O-β-D-[6-(3-hydroxybutanoyl)glucopyranoside] (viscumneoside IX; 1) and homoeriodictyol 7-O-β-D-[6-(3-hydroxybutanoyl)glucopyranosyl](1→2)-β-D-glucopyranoside (viscumneoside X; 2), together with four known flavanoids, 2-homoeriodictyol 7-O-β-D-glucopyranoside (3), viscumneoside I (4), viscumneoside III (5), and 4',5-dihydroxy-3'-methoxy-7-(2-O-α-L-rhamnopyranosyl-β-D-glucopyranosyloxy)flavanone (6) were isolated from stems and leaves of Viscum coloratum. Their structures were elucidated on the basis of their NMR spectra, HR-FAB-MS data, and acid hydrolysis. Inhibitory effects of the four compounds 1-4 on the formation of osteoclast-like multinucleated cells were investigated. As a result, all the four flavanoids showed significant inhibitory effects on the formation of osteoclast-like multinuclear cells even at a low concentration of 2 μg/ml. The activities of 1-4 at such a concentration exceeded or approximated to that of elcitonin, the positive control drug at a concentration of 2 U/ml, suggesting that they may be of interest for the development of new anti-osteoporosis drugs.
authors.affiliation: School of Traditional Chinese Materia Medica 48#, Shenyang Pharmaceutical University, Wenhua Road 103, Shenhe District, Shenyang 110016, P. R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.forename: Ting
authors.initials: T
authors.lastname: Huang
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Kadota
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008968:Molecular Conformation; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D028181:Viscum / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005960:Glucosides; C569137:viscumneoside IX; C569138:viscumneoside X