title: Reveal Salmonella enteritidis test for detection of Salmonella enteritidis in shell eggs and environmental samples.
abstract: Reveal Salmonella Enteritidis (SE) is a lateral flow-based immunodiagnostic assay used for rapid detection of Salmonella enterica serovar Enteritidis from pooled shell eggs and environmental samples. This assay uses highly specific antibodies to accurately detect S. Enteritidis. Studies were conducted to compare the performance of this test against reference procedures for detection of S. Enteritidis from both pooled shell eggs and environmental samples. Pooled shell eggs were inoculated with low levels ofS. Enteritidis and were enriched according to the procedure prescribed by the U.S. Food and Drug Administration. Uninoculated samples were included in each trial. Reveal SE exhibited 100% sensitivity and 100% specificity in comparison to the reference method in all trials. An abbreviated 48 h/(no hold) enrichment procedure was also developed and validated for detection ofS. Enteritidis from pooled shell egg samples. This shortened enrichment procedure can be used in conjunction with the Reveal SE test and offers a significant enrichment time savings of 96 h. Chi-square analysis revealed that there was no significant difference between the abbreviated Reveal method and the reference procedure for detection ofS. Enteritidis from pooled shell egg samples. Out of 245 natural drag swabs screened internally, only three samples tested Reveal SE positive and were confirmed by the reference procedure, resulting in 100% sensitivity and 100% specificity. An external laboratory screened 147 poultry house environmental samples and obtained 35 Reveal SE confirmed positives for Reveal SE sensitivity of 100% and specificity of 90%. Inoculation trials with drag swabs resulted in 96% sensitivity and 100% specificity. Thus, these data demonstrate that Reveal SE is a highly sensitive and specific assay for the detection of S. Enteritidis from both pooled shell eggs and environmental samples.
authors.affiliation: Neogen Corp., 620 Lesher PI, Lansing, MI 48912, USA.
authors.forename: Balamurugan
authors.initials: B
authors.lastname: Jagadeesan
authors.forename: Stephanie
authors.initials: S
authors.lastname: Curry
authors.forename: Debra
authors.initials: D
authors.lastname: Foti
authors.forename: Lauren
authors.initials: L
authors.lastname: Peterson
authors.forename: Rebecca
authors.initials: R
authors.lastname: Wilson
authors.forename: Mark
authors.initials: M
authors.lastname: Mozola
mesh_terms: D000818:Animals; D002645:Chickens; D004783:Environmental Microbiology; D005516:Food Microbiology; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D010063:Ovum / Q000382:microbiology*; D012477:Salmonella enteritidis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity