title: Combined microwave-assisted extraction and high-speed counter-current chromatography for separation and purification of xanthones from Garcinia mangostana.
abstract: A microwave-assisted extraction (MAE) method is presented for the extraction of xanthones, α-mangostin and γ-mangostin from Garcinia mangostana. The MAE conditions including extraction temperature, liquid/solid ratio, extraction time and concentration of ethanol were optimized with an orthogonal test, and 5 g sample was extracted with the optimized conditions. The crude extraction of MAE was successfully isolated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (0.8:0.8:1:0.6, v/v) in one-step separation. The separation yielded 75 mg of α-mangostin at 98.5% purity, and 16 mg of γ-mangostin at 98.1% purity from 360 mg crude extract of G. mangostana in less than 7h. The purity of the two xanthones was determined by HPLC. Their structures were further identified by ESI-MS, (1)H NMR and (13)C NMR.
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Liu
authors.forename: Huiyong
authors.initials: H
authors.lastname: Zhuang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D029762:Garcinia mangostana / Q000737:chemistry*; D008872:Microwaves; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D044004:Xanthones