title: Engineering zinc finger nucleases for targeted mutagenesis of zebrafish.
abstract: Zinc finger nucleases provide an important platform for performing reverse genetics in zebrafish. Here we review various methods and resources that have been used to create customized zinc finger nucleases for use in zebrafish. We also provide a framework for choosing among the various publicly available platforms available to engineer ZFNs.
authors.affiliation: Molecular Pathology Unit, Center for Computational and Integrative Biology, and Center for Cancer Research, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
authors.forename: Jeffry D
authors.initials: JD
authors.lastname: Sander
authors.forename: J-R Joanna
authors.initials: JR
authors.lastname: Yeh
authors.forename: Randall T
authors.initials: RT
authors.lastname: Peterson
authors.forename: J Keith
authors.initials: JK
authors.lastname: Joung
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D003001:Cloning, Molecular; D003663:Decision Trees; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D005822:Genetic Vectors; D016297:Mutagenesis, Site-Directed / Q000379:methods; D015202:Protein Engineering / Q000379:methods*; D059386:Reverse Genetics / Q000379:methods*; D015027:Zebrafish / Q000235:genetics*; D016335:Zinc Fingers
chemical_list: C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific