title: Marked heterogeneity in growth characteristics of myoblast clonal cultures and myoblast mixed cultures obtained from the same individual.
abstract: BACKGROUND
  Sarcopenia is defined as an age-related decrease in skeletal muscle mass and function while adjacent satellite cells are unable to compensate for this loss. However, myoblast cultures can be established even in the presence of sarcopenia.
  
  
  OBJECTIVE
  It is yet unknown whether satellite cells from failing muscle in older age are equally affected, as human satellite cells have been assessed using myoblast mixed cultures and not by using myoblast clonal cultures. We questioned to what extent myoblast mixed cultures reflect the in vivo characteristics of single satellite cells from adult skeletal muscle.
  
  
  METHODS
  We established a myoblast mixed culture and three myoblast clonal cultures out of the same muscle biopsy and cultured these cells for 100 days. Replicative capacity and oxidative stress resistance were compared.
  
  
  RESULTS
  We found marked heterogeneity between the myoblast clonal cultures that all had a significantly lower replicative capacity when compared to the mixed culture. Replicative capacity of the clonal cultures was inversely related to the β-galactosidase activity after exposure to oxidative stress. Addition of L-carnosine enhanced the remaining replicative capacity in all cultures with a concomitant marginal decrease in β-galactosidase activity.
  
  
  CONCLUSIONS
  It is concluded that myoblast mixed cultures in vitro do not reflect the marked heterogeneity between single isolated satellite cells. The consequences of the heterogeneity on muscle performance remain to be established.
authors.affiliation: Department of Gerontology and Geriatrics, Leiden University Medical Centre, Leiden, The Netherlands. a.b.maier @ lumc.nl
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Maier
authors.forename: Ron
authors.initials: R
authors.lastname: Cohen
authors.forename: Joke
authors.initials: J
authors.lastname: Blom
authors.forename: Diana
authors.initials: D
authors.lastname: van Heemst
authors.forename: Rudi G J
authors.initials: RG
authors.lastname: Westendorp
mesh_terms: D002336:Carnosine / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D032448:Myoblasts, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress; D055948:Sarcopenia / Q000378:metabolism / Q000473:pathology; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002336:Carnosine; D001616:beta-Galactosidase