title: Characterization of cathepsin B proteinase (AcCP-2) in eggs and larvae stages of hookworm Ancylostoma caninum.
abstract: Cathepsin B proteinase constitutes a large multigenes family in parasitic and non-parasitic nematodes. The localization of cathepsin B proteinases (AcCP-1 and AcCP-2) in adult worm of Ancylostoma caninum has been characterized (Harrop et al., 1995), but the localization and function in eggs and larval stages remained undiscovered. Here we described the expressing of cathepsin B proteinase (AcCP-2) in Escherichia coli, and immuno-localization of cathepsin B proteinase in eggs and larvae stages of A. caninum. A cDNA fragment encoding a cathepsin B proteinase (AcCP-2) was cloned from A. caninum and expressed in E. coli. Gelatin digestion showed that recombinant cathepsin B proteinase (AcCP-2) has protease activity. The protein level of cathepsin B proteinase in larval and adult worm was detected by western blot. The immuno-localization of cathepsin B proteinase in eggs and larval stages was characterized. The expression of cathepsin B proteinase was more abundant in eggs and larvae stages of A. caninum. It implied that cathepsin B proteinase might play roles in the early development of A. caninum.
authors.affiliation: Key Laboratory of the Ministry of Education for Subtropical Wetland Ecosystem Research, School of Life Science, Xiamen University, Xiamen, Fujian 361005, People's Republic of China. yryang@xmu.edu.cn
authors.forename: Yurong
authors.initials: Y
authors.lastname: Yang
authors.forename: Weiwen
authors.initials: W
authors.lastname: Qin
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Jianxi
authors.initials: J
authors.lastname: Ying
authors.forename: Jing
authors.initials: J
authors.lastname: Zhen
mesh_terms: D000722:Ancylostoma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000724:Ancylostomiasis / Q000469:parasitology / Q000662:veterinary; D000818:Animals; D015153:Blotting, Western; D002401:Cathepsin B / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D007814:Larva / Q000201:enzymology; D008297:Male; D010063:Ovum / Q000201:enzymology; D059748:Proteolysis; D018510:RNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D048348:Reverse Transcription
chemical_list: D018076:DNA, Complementary; D018510:RNA, Helminth; D002401:Cathepsin B