title: Phenotypic and genotypic characterization of dengue virus isolates differentiates dengue fever and dengue hemorrhagic fever from dengue shock syndrome.
abstract: Dengue viruses (DENV) cause 50-100 million cases of acute febrile disease every year, including 500,000 reported cases of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Viral factors have been proposed to influence the severity of the disease, but markers of virulence have never been identified on DENV. Three DENV serotype-1 isolates from the 2007 epidemic in Cambodia that are derived from patients experiencing the various clinical forms of dengue were characterized both phenotypically and genetically. Phenotypic characteristics in vitro, based on replication kinetics in different cell lines and apoptosis response, grouped isolates from DF and DHF patients together, whereas the virus isolate from a DSS patient showed unique features: a lower level of replication in mammalian cells and extensive apoptosis in mosquito cells. Genomic comparison of viruses revealed six unique amino acid residues in the membrane, envelope, and in non-structural genes in the virus isolated from the DSS patient.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Anne
authors.initials: A
authors.lastname: Tuiskunen
authors.forename: Vanessa
authors.initials: V
authors.lastname: Monteil
authors.forename: Sébastien
authors.initials: S
authors.lastname: Plumet
authors.forename: Laetitia
authors.initials: L
authors.lastname: Boubis
authors.forename: Maria
authors.initials: M
authors.lastname: Wahlström
authors.forename: Veasna
authors.initials: V
authors.lastname: Duong
authors.forename: Philippe
authors.initials: P
authors.lastname: Buchy
authors.forename: Ake
authors.initials: A
authors.lastname: Lundkvist
authors.forename: Hugues
authors.initials: H
authors.lastname: Tolou
authors.forename: Isabelle
authors.initials: I
authors.lastname: Leparc-Goffart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002160:Cambodia / Q000453:epidemiology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003715:Dengue / Q000453:epidemiology / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D004196:Disease Outbreaks; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D019595:Severe Dengue / Q000453:epidemiology / Q000821:virology*; D014709:Vero Cells; D014779:Virus Replication