title: Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1).
abstract: The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.
authors.affiliation: Department of Clinical Microbiology, Aarhus University Hospital, Skejby, Brendstrupgaardsvej 100, DK-8200 Aarhus N, Denmark. fuursted@skejby.rm.dk
authors.forename: Kurt
authors.initials: K
authors.lastname: Fuursted
authors.forename: Lone
authors.initials: L
authors.lastname: Schøler
authors.forename: Frank
authors.initials: F
authors.lastname: Hansen
authors.forename: Karen
authors.initials: K
authors.lastname: Dam
authors.forename: Martin S
authors.initials: MS
authors.lastname: Bojer
authors.forename: Anette M
authors.initials: AM
authors.lastname: Hammerum
authors.forename: Frederik
authors.initials: F
authors.lastname: Dagnæs-Hansen
authors.forename: Anders
authors.initials: A
authors.lastname: Olsen
authors.forename: Yousef
authors.initials: Y
authors.lastname: Jasemian
authors.forename: Carsten
authors.initials: C
authors.lastname: Struve
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D001831:Body Temperature; D017173:Caenorhabditis elegans / Q000382:microbiology / Q000502:physiology*; D024901:Drug Resistance, Multiple, Bacterial; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D023421:Models, Animal; D018805:Sepsis / Q000382:microbiology; D014774:Virulence / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1