title: Function of strawberry notch family genes in the zebrafish brain development.
abstract: We previously reported embryonic expression pattern of strawberry notch (sbno) family genes, suggesting involvement in brain development. However function of sbno genes in the vertebrate development has not been known yet. Utilizing zebrafish embryos, we experimentally examined function of sbno genes during brain development in this report. Knockdown experiments of sbno1 and sbno2a disrupted brain morphology, and delayed developmental alteration of gene expression. The earliest effect of loss of function of sbno genes on the zebrafish embryogenesis that we found here was downregulation of otx2 expression. Knockdown of sbno1 specifically affects regionalization along the anterior-posterior axis of the brain. These results suggest essential roles of sbno genes in vertebrate brain development.
authors.affiliation: Division of Anatomy and Neurobiology, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan. ai0926ta@med.kobe-u.ac.jp
authors.forename: Ai
authors.initials: A
authors.lastname: Takano
authors.forename: Riyo
authors.initials: R
authors.lastname: Zochi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hibi
authors.forename: Toshio
authors.initials: T
authors.lastname: Terashima
authors.forename: Yu
authors.initials: Y
authors.lastname: Katsuyama
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D051959:ELAV Proteins / Q000235:genetics / Q000378:metabolism; D000067778:ELAV-Like Protein 3; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009024:Morphogenesis; D012097:Repressor Proteins / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012097:Repressor Proteins; D029961:Zebrafish Proteins; C064045:delta protein; C088635:elavl3 protein, zebrafish; C570136:sbno1 protein, zebrafish; C570137:sbno2a protein, zebrafish