title: Chondroitin sulfate-g-poly(ϵ-caprolactone) co-polymer aggregates as potential targeting drug carriers.
abstract: The aim of this study is to delineate the effect of various amounts of hydrophobic polycaprolactone (PCL) grafted onto three different degrees of methacrylated chondroitin sulfate (CSMA) on chemical-physical properties. The co-polymers were prepared by reacting the modified PCL and the hydrophilic CSMA via a radical reaction (CSMA-PCL). The effect of degree of methacrylation of CSMA and feed ratio between CSMA and PCL on compositions and critical micelle concentrations was systematically studied. The PCL composition of the CSMA-PCL was characterized by (1)H-NMR and FT-IR. The hydrodynamic diameters and morphologies of CSMA-PCL micelles were studied by DLS and TEM. Critical micelle concentrations were determined using pyrene as a probe. Taking one of the CSMA-PCL micelles as an example, a cancer-mediated ligand, folic acid, was linked to the surface. The cellular uptake of the folic acid-linked CSMA-PCL in folate-receptor-overexpressing KB cells was studied by confocal laser scanning microscopy and flow cytometry.
authors.affiliation: a Faculty of Medicinal and Applied Chemistry, School of Life Science, Kaohsiung Medical University , 100 Shih-Chuan 1st Road , Kaohsiung City , 80708 , Taiwan.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
authors.forename: Hsiao-Chen
authors.initials: HC
authors.lastname: Ni
authors.forename: Chia-Chan
authors.initials: CC
authors.lastname: Lin
mesh_terms: D002470:Cell Survival; D002809:Chondroitin Sulfates / Q000138:chemical synthesis / Q000737:chemistry; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D005434:Flow Cytometry; D005492:Folic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D006801:Humans; D057446:Hydrodynamics; D057927:Hydrophobic and Hydrophilic Interactions; D007624:KB Cells; D008422:Materials Testing; D008823:Micelles; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D014803:Vitamin B Complex / Q000008:administration & dosage / Q000493:pharmacokinetics
chemical_list: D004337:Drug Carriers; D008823:Micelles; D011091:Polyesters; D014803:Vitamin B Complex; C016240:polycaprolactone; D002809:Chondroitin Sulfates; D005492:Folic Acid