title: Genotyping and chlorine-resistance of Methylobacterium aquaticum isolated from water samples in Japan.
abstract: For microbial ecological analysis, 14 strains of Methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and RAPD-PCR tests. The ribopatterns after digestion with EcoRI obtained from 14 strains of M. aquaticum were used to divide the strains into two groups (Groups I and II) with a similarity of 55%. From the analysis of RAPD patterns using primer 208, the 14 strains were divided into 3 groups (A-C) based on a homology of 45% or greater, and from that using primer 272, there were 4 groups (A-D) based on a homology of 50% or greater. The chlorine resistance (99.9% CT values) of these isolates was also experimentally confirmed, and we attempted to define the connection between chlorine resistance and the geno-cluster. The average CT value of group I was 0.89 mg•min/l and the average of group II was 0.69 mg•min/l. No remarkable differences in the CT values for the groups were found.
authors.affiliation: School of Life and Environmental Science, Azabu University, Kanagawa, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Furuhata
authors.forename: Azusa U
authors.initials: AU
authors.lastname: Banzai
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawakami
authors.forename: Naoto
authors.initials: N
authors.lastname: Ishizaki
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Keiichi
authors.initials: K
authors.lastname: Goto
authors.forename: Masafumi
authors.initials: M
authors.lastname: Fukuyama
mesh_terms: D002713:Chlorine / Q000494:pharmacology*; D016000:Cluster Analysis; D005838:Genotype; D007564:Japan; D020580:Methylobacterium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D021521:Ribotyping; D014871:Water Microbiology
chemical_list: D002713:Chlorine