title: Rhesus monkey TRIM5α has distinct HIV-1 restriction activity among different mammalian cell lines.
abstract: Rhesus monkey TRIM5α (TRIM5α(rh)), a member of the tripartite motif (TRIM) family, was identified as the main restriction factor responsible for resistance of old world monkey cells to HIV-1 infection. However, the precise mechanism of HIV-1 infection inhibition by TRIM5α remains elusive and appears to be related to some cellular cofactors. Here we reported that TRIM5α(rh) can significantly reduce the infection efficiency of VSV-G pseudotyped HIV-1/MA-YFP virus in human epithelial carcinoma (HeLa) cells, moderately reduce in porcine kidney (PK-15) cells and have no effect on the pseudotyped virus infection in Madin-Darby canine kidney (MDCK) cells. Furthermore, we found that the different HIV-1 restriction activities have no relation with the intracellular localization of TRIM5α(rh). These results indicate that the cellular environment is very important for the efficient anti-HIV-1 activity of TRIM5α(rh). We speculate that some unknown factors required for HIV-1 infection inhibition activity are adequately expressed in HeLa cells, inadequately expressed in PK-15 cells and absent in MDCK cells.
authors.affiliation: The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Xiaohongshan 44, Wuhan 430071, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.forename: Xi-Hui
authors.initials: XH
authors.lastname: Shen
authors.forename: Hui
authors.initials: H
authors.lastname: Qiu
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Rong-Ge
authors.initials: RG
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002144:Callithrix; D003001:Cloning, Molecular; D004285:Dogs; D014644:Genetic Variation; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D008253:Macaca mulatta; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D014161:Transduction, Genetic; D044767:Ubiquitin-Protein Ligases
chemical_list: D011506:Proteins; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases