title: Molecular convergence of bacterial and eukaryotic surface order.
abstract: The conservation of fluidity is a theme common to all cell membranes. In this study, an analysis of lipid packing was conducted via C-laurdan spectroscopy of cell surface membranes prepared from representative species of Bacteria and Eukarya. We found that despite their radical differences in composition (namely the presence and absence of membrane-rigidifying sterol) the membrane order of all taxa converges on a remarkably similar level. To understand how this similarity is constructed, we reconstituted membranes with either bacterial or eukaryotic components. We found that transmembrane segments of proteins have an important role in buffering lipid-mediated packing. This buffering ensures that sterol-free and sterol-containing membranes exhibit similar barrier properties.
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, Dresden 01307, Germany.
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Kaiser
authors.forename: Michal A
authors.initials: MA
authors.lastname: Surma
authors.forename: Florian
authors.initials: F
authors.lastname: Mayer
authors.forename: Ilya
authors.initials: I
authors.lastname: Levental
authors.forename: Michal
authors.initials: M
authors.lastname: Grzybek
authors.forename: Robin W
authors.initials: RW
authors.lastname: Klemm
authors.forename: Sandrine
authors.initials: S
authors.lastname: Da Cruz
authors.forename: Chris
authors.initials: C
authors.lastname: Meisinger
authors.forename: Volker
authors.initials: V
authors.lastname: Müller
authors.forename: Kai
authors.initials: K
authors.lastname: Simons
authors.forename: Daniel
authors.initials: D
authors.lastname: Lingwood
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000166:cytology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D056890:Eukaryota / Q000166:cytology*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D051381:Rats
chemical_list: D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010446:Peptide Fragments