title: Inhibitory effects of silver nanoparticles on H1N1 influenza A virus in vitro.
abstract: Silver nanoparticles have demonstrated efficient inhibitory activities against human immunodeficiency virus (HIV) and hepatitis B virus (HBV). However, the effects of silver nanoparticles against H1N1 influenza A virus remain unexplored. In this study, the interaction of silver nanoparticles with H1N1 influenza A virus was investigated. Silver nanoparticles with mean particle diameters of 10nm were prepared for the hemagglutination inhibition test, the embryo inoculation assay, and the Mosmann-based 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, where these tests were used to determine the inhibitory activity of silver nanoparticles on H1N1 influenza A virus. MDCK cells were used as the infection model. Electron microscopy analysis and flow cytometry assay were used to determine whether silver nanoparticles could reduce H1N1 influenza A virus-induced apoptosis in MDCK cells. This study demonstrates that silver nanoparticles have anti-H1N1 influenza A virus activities. The inhibitory effects of silver nanoparticles on influenza A virus may be a novel clinical strategy for the prevention of influenza virus infection during the early dissemination stage of the virus.
authors.affiliation: Department of Microbiology, Medical College, Dalian University, Dalian 116622, Liaoning, China.
authors.forename: Dong-xi
authors.initials: DX
authors.lastname: Xiang
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Lin
authors.initials: L
authors.lastname: Pang
authors.forename: Cong-long
authors.initials: CL
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis; D002460:Cell Line; D002470:Cell Survival; D002642:Chick Embryo; D004285:Dogs; D005434:Flow Cytometry; D006385:Hemagglutination Inhibition Tests; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron; D012834:Silver / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D000998:Antiviral Agents; D012834:Silver