title: Enhanced immune response against pertussis toxoid by IgA-loaded chitosan-dextran sulfate nanoparticles.
abstract: The objective of the present study was to evaluate immunological activities of chitosan-dextran sulfate (CS-DS) nanoparticle formulation of pertussis toxoid (PTXd) and its combination with a potential immunological adjuvant, immunoglobulin A (IgA). CS-DS nanoparticles were prepared using a complex coacervation (polyelectrolyte complexation) technique. CS-DS nanoparticle formulations with size and zeta potential in a range of 300-350 nm and +40-+55 mV, respectively, were obtained. An entrapment efficiency of more than 90% was obtained for pertussis toxin and IgA in CS-DS nanoparticles. All loaded nanoparticle formulations showed less than 20% of release within 24 h in in vitro release studies. The immunological evaluation of developed formulations in female Balb/c mice groups showed that the CS-DS nanoparticles formulations induced significantly higher serum IgG and IgG1 titers (p < 0.05) as compared with conventional alum-adjuvanted PTXd formulation administered by subcutaneous route. This study indicated the potential of CS-DS nanoparticles to be a simple and effective particulate delivery system with in-built immunological adjuvant property for acellular protein antigens. The study also revealed the potential important role of IgA-loaded CS-DS nanoparticles as a novel immunological adjuvant for vaccine delivery.
authors.affiliation: School of Pharmacy, CHIRI, Western Australia Biomedical Research Institute, Curtin University, Perth, Western Australia 6845, Australia.
authors.forename: Sameer
authors.initials: S
authors.lastname: Sharma
authors.forename: Trilochan Ks
authors.initials: TK
authors.lastname: Mukkur
authors.forename: Heather Ae
authors.initials: HA
authors.lastname: Benson
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000737:chemistry; D000534:Alum Compounds / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D016264:Dextran Sulfate / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D007070:Immunoglobulin A / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D014121:Toxoids / Q000037:antagonists & inhibitors / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014121:Toxoids; C480355:chitosan sulfate; C051093:pertussis toxoid; C041524:aluminum sulfate; D048271:Chitosan; D016264:Dextran Sulfate