title: Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification.
abstract: A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines.
authors.affiliation: Animal Health Research Institute, Council of Agriculture, Executive Yuan, No. 376, Chung-Cheng Road, Tansui, New Taipei City 25158, Taiwan.
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Victor Fei
authors.initials: VF
authors.lastname: Pang
authors.forename: Chian-Ren
authors.initials: CR
authors.lastname: Jeng
authors.forename: Fan
authors.initials: F
authors.lastname: Lee
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Huang
authors.forename: Yeou-Liang
authors.initials: YL
authors.lastname: Lin
authors.forename: Shih-Hsuan
authors.initials: SH
authors.lastname: Hsiao
authors.forename: Shiow-Suey
authors.initials: SS
authors.lastname: Lai
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control / Q000821:virology*; D014765:Viral Vaccines / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D014765:Viral Vaccines