title: Association of eNOS gene polymorphisms with essential hypertension in the Han population in southwestern China.
abstract: Endothelial  nitric oxide synthase (eNOS) plays an important role in maintaining blood  pressure homeostasis and vascular integrity. Polymorphisms in the eNOS gene  have been found to be associated with hypertension in different human  populations, including Northern and Southern Chinese Han populations. To  examine the relationship of three eNOS gene polymorphisms, T-786C  (rs2070744), G894T (rs1799983), and G10T (rs7830), with hypertension in the Han  population in southwestern China, we carried out a study of the genotypes of  three SNPs in 510 hypertensive and 510 normotensive subjects from the Yunnan  Province by using PCR-RFLP and sequencing. Our SNP analyses showed that the  distribution of the T-786C polymorphism did not differ between patients and  controls, and that G894T and G10T are significantly associated with  hypertension in females, adjusted for covariates. Compared with the other  haplotypes, haplotype H1 (TGG), carrying protective 10G and 894G alleles,  significantly decreased the risk of increased essential hypertension in  females, with an odds ratio of 0.68 (P = 10(-5)). These results  suggest that the eNOS polymorphism is one of the factors contributing to  the predisposition for essential hypertension in the Han population in  southwestern China.
authors.affiliation: Laboratory for Conservation and Utilization of Bio-Resources and Human Genetics Center, Yunnan University, Kunming, Yunnan, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Cun
authors.forename: W R
authors.initials: WR
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S N
authors.initials: SN
authors.lastname: Li
authors.forename: H R
authors.initials: HR
authors.lastname: Ouyang
authors.forename: Y R
authors.initials: YR
authors.lastname: Wu
authors.forename: H J
authors.initials: HJ
authors.lastname: Yu
authors.forename: C J
authors.initials: CJ
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People; D001483:Base Sequence; D001794:Blood Pressure / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D006973:Hypertension / Q000453:epidemiology / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D012737:Sex Factors
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III