title: The inhibiting activity of areca inflorescence extracts on human low density lipoprotein oxidation induced by cupric ion.
abstract: The oxidative modification of human low density lipoprotein (LDL) plays a significant role in atherosclerosis. In this study, the inhibiting activity of areca inflorescence extracts (AIEs) on LDL oxidation was investigated by an in vitro study with Trolox as the standard antioxidant. The kinetics of LDL oxidation, thiobarbituric acid reactive substances assay, ferric-reducing antioxidant power assay and copper chelation assay were also evaluated to assess the antioxidant activities of AIEs, and the results revealed that AIEs could delay the lag time and inhibit the formation of malondialdehyde in the process of LDL peroxidation induced by Cu(2+). The boiled water extract displayed the highest antioxidant activity compared with the ambient water extract and ethanol extract. The total phenolic contents and phenolic components of AIEs were also measured by high performance liquid chromatography method. Epicatechin, gallic acid and coumalic acid were the primary phenolic acids in AIEs.
authors.affiliation: Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, P.R. China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.forename: Yulin
authors.initials: Y
authors.lastname: Huang
authors.forename: Fangfang
authors.initials: F
authors.lastname: Cheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.forename: Rencai
authors.initials: R
authors.lastname: Wang
authors.forename: Minmin
authors.initials: M
authors.lastname: Tang
authors.forename: Yajun
authors.initials: Y
authors.lastname: Zheng
authors.forename: Songlin
authors.initials: S
authors.lastname: Zhao
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001114:Areca / Q000737:chemistry*; D002392:Catechin / Q000032:analysis / Q000494:pharmacology; D002614:Chelating Agents / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D005707:Gallic Acid / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D056626:Inflorescence; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism*; D010636:Phenols / Q000032:analysis / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011753:Pyrones / Q000032:analysis / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D008077:Lipoproteins, LDL; D010636:Phenols; D010936:Plant Extracts; D011753:Pyrones; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D005707:Gallic Acid; D003300:Copper; D002392:Catechin; C052808:coumalic acid