title: Development and implication of a capillary electrophoresis methodology for ciprofloxacin, paracetamol and diclofenac sodium in pharmaceutical formulations and simultaneously in human urine samples.
abstract: This work studies the development of a simple and fairly rapid methodology for simultaneous determination/separation of three frequently co-administered drugs; ciprofloxacin (CIP), paracetamol (PCT) and diclofenac sodium (DIC) using capillary electrophoresis (CE) with UV detection at 260 nm. Separation was achieved in only 6.5 min with a simple buffer of sodium tetraborate (50 mM) at pH 9.0. The Parameters affecting the separation and detection were optimized. The calibration curves were linear in the range of 5-500 µg/mL for CIP, 5-250 µg/mL for PCT and 1-125 µg/mL for DIC sodium under the optimized conditions. The lower limit of detection (LOD) was found to be 1 µg/mL for CIP & PCT and 0.5 µg/mL for DIC. The method was successfully used for the analysis of drugs in commercial pharmaceutical formulations and simultaneously from patient's urine sample with RSD 0.5-2.4%. Results obtained with CE method are compared with standard HPLC procedure and were found in good agreement.
authors.affiliation: National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan. ambersolangi@gmail.com
authors.forename: Amber Rehana
authors.initials: AR
authors.lastname: Solangi
authors.forename: Saima Qayyum
authors.initials: SQ
authors.lastname: Memon
authors.forename: Arfana
authors.initials: A
authors.lastname: Mallah
authors.forename: Najma
authors.initials: N
authors.lastname: Memon
authors.forename: Muhammad Yar
authors.initials: MY
authors.lastname: Khuhawar
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Bhanger
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage / Q000032:analysis* / Q000652:urine*; D002021:Buffers; D002138:Calibration; D002939:Ciprofloxacin / Q000008:administration & dosage / Q000032:analysis* / Q000652:urine*; D004008:Diclofenac / Q000008:administration & dosage / Q000032:analysis* / Q000652:urine*; D004560:Electricity; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000737:chemistry; D013997:Time Factors
chemical_list: D002021:Buffers; D013607:Tablets; D004008:Diclofenac; D000082:Acetaminophen; D002939:Ciprofloxacin