title: Establishment of chemiluminescence enzyme immunoassay for apolipoprotein B-48 and its clinical applications for evaluation of impaired chylomicron remnant metabolism.
abstract: BACKGROUND
  Apolipoprotein B-48 (apoB-48) is a constituent of chylomicron remnants synthesized in the small intestines. The serum concentration of apoB-48 at fasting has been reported to be a marker of postprandial hyperlipidemia, a presumed risk factor for atherosclerosis.
  
  
  METHODS
  We evaluated the basal performance of a recently developed chemiluminescent enzyme immunoassay (CLEIA). We also examined the correlations between serum apoB-48 concentrations and other lipid concentrations or life style patterns, including smoking and drinking. We analyzed the data of 273 clinical samples by multiple regression analysis to examine the influence of other serum lipid values, age, sex, smoking, drinking status and BMI on serum apoB-48 values.
  
  
  RESULTS
  Within-run and between-run precision was obtained with 1.7-2.7% and 1.2-7.3%, respectively. The correlativity of enzyme-linked immunosorbent assay was correlation coefficient r=0.953, and regression y=1.02×-1.59. Serum apoB-48 concentrations were higher in males than in females, and were correlated with the status of smoking as well as with remnant-like particle-cholesterol (RLP-C) concentrations. Patients with the metabolic syndrome showed higher values of serum apoB-48 compared with control subjects.
  
  
  CONCLUSIONS
  Serum apoB-48 measurement by CLEIA was satisfactory for clinical use to assess abnormalities in the chylomicron remnant metabolism.
authors.affiliation: Division of Laboratory for Clinical Investigation, Department of Medical Technology, Osaka University Hospital, Suita, Osaka, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hanada
authors.forename: Satomi
authors.initials: S
authors.lastname: Mugii
authors.forename: Manabu
authors.initials: M
authors.lastname: Okubo
authors.forename: Ikuhiro
authors.initials: I
authors.lastname: Maeda
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kuwayama
authors.forename: Yoh
authors.initials: Y
authors.lastname: Hidaka
authors.forename: Rika
authors.initials: R
authors.lastname: Kitazume-Taneike
authors.forename: Taiji
authors.initials: T
authors.lastname: Yamashita
authors.forename: Ryota
authors.initials: R
authors.lastname: Kawase
authors.forename: Hajime
authors.initials: H
authors.lastname: Nakaoka
authors.forename: Miwako
authors.initials: M
authors.lastname: Inagaki
authors.forename: Miyako
authors.initials: M
authors.lastname: Yuasa-Kawase
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakatani
authors.forename: Kazumi
authors.initials: K
authors.lastname: Tsubakio-Yamamoto
authors.forename: Daisaku
authors.initials: D
authors.lastname: Masuda
authors.forename: Tohru
authors.initials: T
authors.lastname: Ohama
authors.forename: Akifumi
authors.initials: A
authors.lastname: Matsuyama
authors.forename: Masato
authors.initials: M
authors.lastname: Ishigami
authors.forename: Makoto
authors.initials: M
authors.lastname: Nishida
authors.forename: Issei
authors.initials: I
authors.lastname: Komuro
authors.forename: Shizuya
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D053283:Apolipoprotein B-48 / Q000097:blood*; D002914:Chylomicrons / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood; D015203:Reproducibility of Results
chemical_list: D053283:Apolipoprotein B-48; D002914:Chylomicrons