title: The use of myristic acid as a ligand of polyethylenimine/DNA nanoparticles for targeted gene therapy of glioblastoma.
abstract: To establish a gene delivery system for brain targeting, a low molecular weight polyethylenimine (PEI(10 K))  was modified with myristic acid (MC), and complexed with DNA, yielding MC-PEI(10 K)/DNA  nanoparticles successfully. The nanoparticles were observed to be successfully taken up by the brains of mice. The transfection efficiency of the nanoparticles was then investigated, and both the in vitro and in vivo gene expression of MC-PEI(10 K)/DNA  nanoparticles is significantly higher than that of unmodified PEI(10 K)/DNA  nanoparticles. The anti-glioblastoma effect of MC-PEI(10 K)/pORF-hTRAIL was demonstrated by the survival time of intracranial U87 glioblastoma-bearing mice. The median survival time of the MC-PEI(10 K)/pORF-hTRAIL group (28 days) was significantly longer than that of the PEI(10 K)/pORF-hTRAIL group (24 days), the MC-PEI(10 K)/pGL(3)  group (21 days) and the saline group (22 days). Therefore, our results suggested that MC-PEI(10 K)  could be potentially used for brain-targeted gene delivery and in the treatment of glioblastoma.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Gu
authors.forename: Qinggang
authors.initials: Q
authors.lastname: Meng
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yan
authors.forename: Huile
authors.initials: H
authors.lastname: Gao
authors.forename: Xishan
authors.initials: X
authors.lastname: Chen
authors.forename: Xiangkun
authors.initials: X
authors.lastname: Yang
authors.forename: Weiyue
authors.initials: W
authors.lastname: Lu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D045744:Cell Line, Tumor; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005909:Glioblastoma / Q000235:genetics* / Q000628:therapy; D006801:Humans; D051379:Mice; D019814:Myristic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics; D014162:Transfection
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; D019814:Myristic Acid; D011094:Polyethyleneimine; D004247:DNA