title: Overexpression of cyclin E isoforms correlates with poor prognosis in rectal cancer.
abstract: OBJECTIVE
  To investigate the expression of cyclin E isoforms in rectal cancer and its relations to clinicopathological factors and survival.
  
  
  METHODS
  Cyclin E expression was assessed by Western blot in 360 resected rectal cancer patients of stage I to III. Multivariate analysis was applied to indicate the independent prognostic markers in this cohort.
  
  
  RESULTS
  Nineteen percent, 24% or 29% patients exhibited elevated levels of full-length (FL) cyclin E, low-molecular-weight (LMW) cyclin E or total cyclin E in their tumors respectively. Significant correlation was observed between cyclin E expression with blood vessel invasion, deeply invasive tumors, histology grade and lymph node metastasis. Moreover, patients with high levels of LMW-cyclin E or total cyclin E had a poorer 5-year overall survival than did patients with low levels of LMW-cyclin E or total cyclin E. In multivariate analysis, both the LMW-cyclin E and total cyclin E, but not FL-cyclin E, remained independent prognostic indicators in both patients with stage I to III and in those with early stage. Patients with elevated LMW- or total cyclin E levels had a hazard ratio for death from rectal cancer of 6.302 (95% CI, 1.903-17.81, p = 0.001) or 4.332 (95% CI, 1.298-16.362, p = 0.001).
  
  
  CONCLUSIONS
  Overexpression of the LMW-cyclin E or total cyclin E is a strong predictor for poorer survival in patients with rectal cancer. Therefore, evaluating cyclin E expression may provide useful prognostic information for resectable rectal cancer patients.
authors.affiliation: Department of Oncology, Union Hospital of Fujian Medical University, Fujian, PR China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Y T
authors.initials: YT
authors.lastname: Xie
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: L
authors.initials: L
authors.lastname: Yan
authors.forename: G X
authors.initials: GX
authors.lastname: Guan
authors.forename: X
authors.initials: X
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry / Q000209:etiology / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000378:metabolism*; D015153:Blotting, Western; D019927:Cyclin E / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D020033:Protein Isoforms / Q000378:metabolism; D012004:Rectal Neoplasms / Q000737:chemistry / Q000209:etiology / Q000378:metabolism* / Q000401:mortality* / Q000473:pathology; D018570:Risk Assessment; D012307:Risk Factors; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D019927:Cyclin E; D020033:Protein Isoforms