title: Do mosquito-associated bacteria of the genus Asaia circulate in humans?
abstract: Symbiotic bacteria of the genus Asaia have been proposed as tools for control of mosquito-borne diseases, specifically malaria. However, safety issues are a major concern for paratransgenesis strategies. The aim of this study is to investigate, with immunofluorescence assays and quantitative PCR experiments, whether Asaia spp. is circulating among humans. All human sera and whole blood samples analyzed were negative for Asaia spp., thus suggesting that this organism could be utilized, in the future, as a malaria control tool.
authors.affiliation: Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università di Camerino, Via Gentile III da Varano, 62032, Camerino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Epis
authors.forename: P
authors.initials: P
authors.lastname: Gaibani
authors.forename: U
authors.initials: U
authors.lastname: Ulissi
authors.forename: B
authors.initials: B
authors.lastname: Chouaia
authors.forename: I
authors.initials: I
authors.lastname: Ricci
authors.forename: C
authors.initials: C
authors.lastname: Damiani
authors.forename: V
authors.initials: V
authors.lastname: Sambri
authors.forename: F
authors.initials: F
authors.lastname: Castelli
authors.forename: F
authors.initials: F
authors.lastname: Buelli
authors.forename: D
authors.initials: D
authors.lastname: Daffonchio
authors.forename: C
authors.initials: C
authors.lastname: Bandi
authors.forename: G
authors.initials: G
authors.lastname: Favia
mesh_terms: D016947:Acetobacteraceae / Q000302:isolation & purification*; D000818:Animals; D001769:Blood / Q000382:microbiology*; D001782:Blood Donors; D009033:Culicidae / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction