title: Viability of maxillary bone harvesting by using different osteotomy techniques. A pilot study.
abstract: The use of autogenous grafts is still considered in bone regeneration surgeries. However, the bone cell viability of such grafts after being harvested from donor sites remains a matter of debate. The aim of the present study is to evaluate particulated and block bone cell viability, in terms of presence or absence of apoptosis and necrosis, obtained from different maxillary intra-oral harvesting methods: bone scraper, rotary carbide burs and piezoelectric device. Five healthy patients were enrolled in the study. The patients required sinus augmentation by lateral window approach. The bone was harvested by the bone scraper, piezoelectric device and rotary surgical instrument. The samples were processed with the Annexin V/FITC (fluorescein isothiocyanate stain) kit and were analyzed by means of Fluoresence-Activated Cell Sorted (FACS) technique. Within the limitations of this pilot study, the results indicated that autogenous bone chips collected from the three harvesting methods presented a large percentage of apoptotic cells, although large scale production of necrotic cells was not detected. In summary, although rotary surgical instrument and piezoelectric devices are frequently used instruments for oral osteotomy, fresh autogenous bone chips collected from them did not present a viable bone cell source.
authors.affiliation: Laboratory of Regenerative Medicine, Faculty of Dentistry, International University of Catalonia, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Atari
authors.forename: P
authors.initials: P
authors.lastname: Chatakun
authors.forename: O
authors.initials: O
authors.lastname: Ortiz
authors.forename: A
authors.initials: A
authors.lastname: Mañes
authors.forename: C
authors.initials: C
authors.lastname: Gil-Recio
authors.forename: M Fabregat
authors.initials: MF
authors.lastname: Navarro
authors.forename: D A
authors.initials: DA
authors.lastname: Garcia-Fernández
authors.forename: J
authors.initials: J
authors.lastname: Caballé-Serrano
authors.forename: J
authors.initials: J
authors.lastname: Mareque
authors.forename: F
authors.initials: F
authors.lastname: Hernández-Alfaro
authors.forename: E
authors.initials: E
authors.lastname: Ferrés Padró
authors.forename: L
authors.initials: L
authors.lastname: Giner-Tarrida
mesh_terms: D000328:Adult; D017209:Apoptosis; D016025:Bone Transplantation; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D004867:Equipment Design; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008437:Maxilla / Q000637:transplantation*; D008875:Middle Aged; D009336:Necrosis; D010027:Osteotomy / Q000295:instrumentation; D010865:Pilot Projects; D013030:Spain; D013525:Surgical Instruments; D020858:Tissue and Organ Harvesting / Q000295:instrumentation / Q000379:methods*