title: Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats.
abstract: BACKGROUND
  Pulsed electromagnetic fields (PEMF) stimulation has been used successfully to treat nonunion fractures and femoral head osteonecrosis, but relatively little is known about its effects on preventing steroid-induced osteonecrosis. The purpose of the study was to investigate the effects of PEMF stimulation on the prevention of steroid-induced osteonecrosis in rats and explore the underlying mechanisms.
  
  
  METHODS
  Seventy-two male adult Wistar rats were divided into three groups and treated as follows. (1) PEMF stimulation group (PEMF group, n = 24): intravenously injected with lipopolysaccharide (LPS, 10 μg/kg) on day 0 and intramuscularly injected with methylprednisolone acetate (MPSL, 20 mg/kg) on days 1, 2 and 3, then subjected to PEMF stimulation 4 h per day for 1 to 8 weeks. (2) Methylprednisolone-treated group (MPSL group, n = 24): injected the same dose of LPS and MPSL as the PEMF group but without exposure to PEMF. (3) Control group (PS group, n = 24): injected 0.9% saline in the same mode at the same time points. The incidence of osteonecrosis, serum lipid levels and the mRNA and protein expression of transforming growth factor β1 (TGF-β1) in the proximal femur were measured 1, 2, 4 and 8 weeks after the last MPSL (or saline) injection.
  
  
  RESULTS
  The incidence of osteonecrosis in the PEMF group (29%) was significantly lower than that observed in the MPSL group (75%), while no osteonecrosis was observed in the PS group. The serum lipid levels were significantly lower in the PEMF and PS groups than in the MPSL group. Compared with the MPSL and PS groups, the mRNA expression of TGF-β1 increased, reaching a peak 1 week after PEMF treatment, and remained high for 4 weeks, then declined at 8 weeks, whereas the protein expression of TGF-β1 increased, reaching a peak at 2 weeks after PEMF treatment, and remained high for 8 weeks.
  
  
  CONCLUSIONS
  PEMF stimulation can prevent steroid-induced osteonecrosis in rats, and the underlying mechanisms involve decreased serum lipid levels and increased expression of TGF-β1.
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, People's Republic of China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Ding
authors.forename: Hao
authors.initials: H
authors.lastname: Peng
authors.forename: Hong-Song
authors.initials: HS
authors.lastname: Fang
authors.forename: Jian-Lin
authors.initials: JL
authors.lastname: Zhou
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004574:Electromagnetic Fields; D015870:Gene Expression / Q000528:radiation effects; D005938:Glucocorticoids / Q000494:pharmacology; D007275:Injections, Intravenous; D008055:Lipids / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D055909:Magnetic Field Therapy / Q000379:methods*; D008297:Male; D008775:Methylprednisolone / Q000031:analogs & derivatives / Q000494:pharmacology; D000077555:Methylprednisolone Acetate; D010020:Osteonecrosis / Q000139:chemically induced / Q000378:metabolism / Q000532:radiotherapy*; D061208:Pulsed Radiofrequency Treatment; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D005938:Glucocorticoids; D008055:Lipids; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D000077555:Methylprednisolone Acetate; D008775:Methylprednisolone