title: Evaluation of the Clearview® Malaria pLDH Malaria Rapid Diagnostic Test in a non-endemic setting.
abstract: BACKGROUND
  Malaria Rapid Diagnostic Tests (RDTs) are widely used to diagnose malaria. The present study evaluated a new RDT, the Clearview® Malaria pLDH test targeting the pan-Plasmodium antigen lactate dehydrogenase (pLDH).
  
  
  METHODS
  The Clearview® Malaria pLDH test was evaluated on fresh samples obtained in returned international travellers using microscopy corrected by PCR as the reference method. Included samples were Plasmodium falciparum (139), Plasmodium vivax (22), Plasmodium ovale (20), Plasmodium malariae (7), and 102 negative.
  
  
  RESULTS
  Overall sensitivity for the detection of Plasmodium spp was 93.2%. For P. falciparum, the sensitivity was 98.6%; for P. vivax, P. ovale and P. malariae, overall sensitivities were 90.9%, 60.0% and 85.7% respectively. For P. falciparum and for P. vivax, the sensitivities increased to 100% at parasite densities above 100/μl. The specificity was 100%. The test was easily to perform and the result was stable for at least 1 hour.
  
  
  CONCLUSIONS
  The Clearview® Malaria pLDH was efficient for the diagnosis of malaria. The test was very sensitive for P. falciparum and P. vivax detection. The sensitivities for P. ovale and P. malariae were better than other RDTs.
authors.affiliation: Laboratory of Parasitology, Malaria National Reference Centre, AP-HP, Bichat Hospital, 46 rue Henri Huchard, 75018 Paris, France. sandrine.houze@bch.aphp.fr
authors.forename: Sandrine
authors.initials: S
authors.lastname: Houzé
authors.forename: Véronique
authors.initials: V
authors.lastname: Hubert
authors.forename: Dorit Pessler
authors.initials: DP
authors.lastname: Cohen
authors.forename: Baruch
authors.initials: B
authors.lastname: Rivetz
authors.forename: Jacques
authors.initials: J
authors.lastname: Le Bras
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000953:Antigens, Protozoan / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008288:Malaria / Q000175:diagnosis*; D008297:Male; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D007770:L-Lactate Dehydrogenase