title: Nitidine Chloride inhibits breast cancer cells migration and invasion by suppressing c-Src/FAK associated signaling pathway.
abstract: Nitidine is a benzophenanthridine alkaloid, which has been shown to have anti-tumor properties. Here, we demonstrated that Nitidine Chloride (NC) could inhibit breast cancer cells migration and invasion both in vitro and in vivo. Meanwhile, the protrusion formation and partial proteolytic activity of MMP-9 and MMP-2 were attenuated by NC in a dose-dependent manner in MDA-MB-231 cells. Furthermore, addition NC to cells significantly decreases PDGF induced phosphorylation of c-Src, FAK, MAPKs, activation of RhoA, Rac1 and AP-1 transcriptional activity. Taken together, our results indicate that NC could have potential as a novel anti-metastasis drug to breast cancer.
authors.affiliation: The Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Pan
authors.forename: Honghui
authors.initials: H
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Linli
authors.initials: L
authors.lastname: Yang
authors.forename: Ruimei
authors.initials: R
authors.lastname: Li
authors.forename: Zhenxi
authors.initials: Z
authors.lastname: Li
authors.forename: Junchen
authors.initials: J
authors.lastname: Liu
authors.forename: Qufei
authors.initials: Q
authors.lastname: Zhao
authors.forename: Min
authors.initials: M
authors.lastname: Qian
authors.forename: Mingyao
authors.initials: M
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Du
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D053119:Benzophenanthridines / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D000081247:CSK Tyrosine-Protein Kinase; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D010982:Platelet-Derived Growth Factor / Q000633:toxicity; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism; D020830:rac1 GTP-Binding Protein / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism; D019061:src-Family Kinases
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053119:Benzophenanthridines; D010982:Platelet-Derived Growth Factor; C107953:RAC1 protein, human; D018808:Transcription Factor AP-1; C013615:nitidine; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D019061:src-Family Kinases; C000631346:CSK protein, human; D048049:Extracellular Signal-Regulated MAP Kinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D020830:rac1 GTP-Binding Protein; D020742:rhoA GTP-Binding Protein