title: Mitochondrial dysfunction resulting from the absence of mitochondrial porin in Neurospora crassa.
abstract: Porin, the voltage-dependent anion-selective channel (VDAC) in the mitochondrial outer membrane, contributes to metabolism and apoptosis. VDAC function was investigated in Neurospora, an obligate aerobe with a single porin. Porinless strains are viable, with cold-sensitive growth, cytochrome deficiencies and overexpression of alternative oxidase. iTRAQ labeling of mitochondria from a porinless strain and its progenitor revealed a small group of proteins with altered expression levels in the mutant organelles. Porinless Neurospora appears to compensate not by inducing alternative pores, but by altering electron flow and nucleotide metabolism. Transcriptional and post-transcriptional mechanisms contribute to the response, reflecting the extent of porin influence.
authors.affiliation: Department of Microbiology, University of Manitoba, 301 Buller Building, Winnipeg, MB, Canada R3T 2N2. hopperq2@gmail.com
authors.forename: William A T
authors.initials: WA
authors.lastname: Summers
authors.forename: John A
authors.initials: JA
authors.lastname: Wilkins
authors.forename: Ravi C
authors.initials: RC
authors.lastname: Dwivedi
authors.forename: Peyman
authors.initials: P
authors.lastname: Ezzati
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Court
mesh_terms: D003080:Cold Temperature; D003580:Cytochromes / Q000172:deficiency; D004579:Electron Transport; D015870:Gene Expression; D050296:Microbial Viability; D008928:Mitochondria / Q000502:physiology*; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism; D010088:Oxidoreductases / Q000096:biosynthesis; D010940:Plant Proteins / Q000096:biosynthesis; D018272:Porins / Q000235:genetics* / Q000378:metabolism*; D000099188:Alternative Oxidase
chemical_list: D003580:Cytochromes; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D009711:Nucleotides; D010088:Oxidoreductases; D010940:Plant Proteins; D018272:Porins; D000099188:Alternative Oxidase