title: Phenotyping of P105-negative B cell subsets in patients with systemic lupus erythematosus.
abstract: This study aimed to investigate phenotype of RP105(-) B cell subsets in patients with systemic lupus erythematosus (SLE). Flow cytometry was used for phenotyping RP105-negaive B cell subsets. Based on CD19, RP105, and CD138 expression, RP105(-) B cells consist of at least 5 subsets of late B cells, including CD19(+)RP105(int), CD19(+) RP105(-), CD19(low) RP105(-) CD138(-), CD19(low) RP105(-)CD138(int), and CD19(low) RP105(-) CD138(++) B cells. Especially, CD19(+)RP105(int) and CD19(low) RP105(-)CD138(int) B cells are significantly larger than other RP105(-) B cell subsets in SLE. By comparison of RP105(-) B cell subsets between patients with SLE and normal subjects, these subsets were detectable even in normal subjects, but the percentages of RP105(-) B cell subsets were significantly larger in SLE. The phenotypic analysis of RP105(-) B cell subsets suggests dysregulation of later B cell subsets in SLE and may provide new insights into understanding regulation of B cells in human SLE.
authors.affiliation: Division of Rheumatology, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan. koarada@post.saga-med.ac.jp
authors.forename: Syuichi
authors.initials: S
authors.lastname: Koarada
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Tada
authors.forename: Rie
authors.initials: R
authors.lastname: Suematsu
authors.forename: Sachiko
authors.initials: S
authors.lastname: Soejima
authors.forename: Hisako
authors.initials: H
authors.lastname: Inoue
authors.forename: Akihide
authors.initials: A
authors.lastname: Ohta
authors.forename: Kohei
authors.initials: K
authors.lastname: Nagasawa
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D034961:Antigens, Nuclear / Q000235:genetics / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D002868:Chromosomal Proteins, Non-Histone / Q000172:deficiency* / Q000235:genetics / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D015703:Antigens, CD; D034961:Antigens, Nuclear; D002868:Chromosomal Proteins, Non-Histone; C478629:P105 antigen, human