title: Two-stage acid saccharification of fractionated Gelidium amansii minimizing the sugar decomposition.
abstract: Two-stage acid hydrolysis was conducted on easy reacting cellulose and resistant reacting cellulose of fractionated Gelidium amansii (f-GA). Acid hydrolysis of f-GA was performed at between 170 and 200 °C for a period of 0-5 min, and an acid concentration of 2-5% (w/v, H2SO4) to determine the optimal conditions for acid hydrolysis. In the first stage of the acid hydrolysis, an optimum glucose yield of 33.7% was obtained at a reaction temperature of 190 °C, an acid concentration of 3.0%, and a reaction time of 3 min. In the second stage, a glucose yield of 34.2%, on the basis the amount of residual cellulose from the f-GA, was obtained at a temperature of 190 °C, a sulfuric acid concentration of 4.0%, and a reaction time 3.7 min. Finally, 68.58% of the cellulose derived from f-GA was converted into glucose through two-stage acid saccharification under aforementioned conditions.
authors.affiliation: Department of Applied Chemical Engineering, Dankook University, Cheonan, Chungnam 330-714, Republic of Korea.
authors.forename: Tae Su
authors.initials: TS
authors.lastname: Jeong
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Kim
authors.forename: Kyeong Keun
authors.initials: KK
authors.lastname: Oh
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D005947:Glucose / Q000032:analysis; D006868:Hydrolysis / Q000187:drug effects; D000461:Rhodophyta / Q000187:drug effects* / Q000378:metabolism*; D013464:Sulfuric Acids / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013464:Sulfuric Acids; D005947:Glucose; C033158:sulfuric acid