title: Mnl2, a novel component of the ER associated protein degradation pathway.
abstract: In eukaryotes, membrane and soluble proteins of the secretory pathway enter the endoplasmic reticulum (ER) after synthesis in an unfolded state. Directly after entry, most proteins are modified with glycans at suitable glycosylation sites and start to fold. A protein that cannot fold properly will be degraded in a process called ER associated degradation (ERAD). Failures in ERAD, either by loss of function or by premature degradation of proteins, are a cause of severe diseases. Therefore, the search for novel ERAD components to gain better insight in this process is of high importance. Carbohydrate trimming is a relevant process in ER quality control. In this work a novel putative yeast mannosidase encoded by the open reading frame YLR057W was identified and named Mnl2. Deletion of MNL2 diminished the degradation efficiency of misfolded CPY(*) in the absence of the cognate mannosidase Mnl1, indicating a specific role in ERAD.
authors.affiliation: Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany.
authors.forename: Elena
authors.initials: E
authors.lastname: Martinez Benitez
authors.forename: Alexandra
authors.initials: A
authors.lastname: Stolz
authors.forename: Alexander
authors.initials: A
authors.lastname: Becher
authors.forename: Dieter H
authors.initials: DH
authors.lastname: Wolf
mesh_terms: D002268:Carboxypeptidases / Q000737:chemistry / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D017353:Gene Deletion; D008361:Mannosidases / Q000235:genetics / Q000378:metabolism*; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D008361:Mannosidases; C568708:Mnl2 protein, S cerevisiae; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D002268:Carboxypeptidases; C520242:CPY protein, S cerevisiae