title: Incorporation of unnatural non-α-amino acids into the N-terminus of proteins in a cell-free translation system.
abstract: Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan. hohsaka@jaist.ac.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hohsaka
mesh_terms: D000215:Acylation; D000596:Amino Acids / Q000737:chemistry*; D001483:Base Sequence; D002474:Cell-Free System; D017931:DNA Primers; D005456:Fluorescent Dyes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D017931:DNA Primers; D005456:Fluorescent Dyes; D011506:Proteins