title: Electrochemical aptamer sensor for small molecule assays.
abstract: Detection and quantification of small molecules have played essential roles in environmental analysis and clinical diagnosis. Aptamers are oligonucleic acids that bind to a specific target molecule with high specificity and affinity which are promising features for sensing small molecules. Electrochemical detection is an attractive way to exploit aptamer sensors (aptasensors) because of its high sensitivity, simple instrumentation, low cost, fast response and portability. Herein, we describe a label-free small molecular aptasensor based on a signal-amplification mechanism which uses gold nanoparticles. This aptasensor can selectively detect low nanomolar levels of ATP, the example target compound.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.forename: Xiahong
authors.initials: X
authors.lastname: Xu
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhou
authors.initials: Z
authors.lastname: Nie
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D006046:Gold; D000255:Adenosine Triphosphate; D004247:DNA