title: miRNA-195 sensitizes human hepatocellular carcinoma cells to 5-FU by             targeting BCL-w.
abstract: The role of microRNA-195 in developing acquired drug resistance in hepatocellular             carcinoma cells was investigated. Expression proﬁling of miRNAs revealed a limited             set of miRNAs with altered expression in drug resistant hepatocellular carcinoma             cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time             PCR confirmed down-regulation of miRNA-195 in BEL-7402/5-FU cells. Overexpression             of miRNA-195 sensitized BEL-7402/5-FU cells to anticancer drugs. Consistent with             these findings, miR-195 antisense oligonucleotide induced drug resistance in BEL-7402/5-FU             cells. Also, the basal levels of the anti-apoptotic protein Bcl-w were high in             BEL-7402/5-FU cells and miR-195 overexpression repressed Bcl-w protein level and             inhibited the luciferase activity of a Bcl-w 3' untranslated region-based reporter             construct in both BEL-7402/5-FU and BEL-7402 cells. These results indicate that             miR-195 could improve the drug sensitivity at least in part by targeting Bcl-w             to increase cell apoptosis in hepatocellular carcinoma cells.
authors.affiliation: Institute of Pharmacy and Pharmacology, University of South China, and The First Affiliated Hospital of University of South China, 28 Changsheng Road, Hengyang, Hunan 421001, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.forename: Qiong
authors.initials: Q
authors.lastname: Xiang
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Liu
authors.forename: Shensong
authors.initials: S
authors.lastname: Tang
authors.forename: Duanfang
authors.initials: D
authors.lastname: Liao
authors.forename: Bingyang
authors.initials: B
authors.lastname: Zhu
authors.forename: Xuyu
authors.initials: X
authors.lastname: Zu
authors.forename: Huifang
authors.initials: H
authors.lastname: Tang
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Lei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; C491073:BCL2L2 protein, human; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D005472:Fluorouracil