title: Reptilian-transcriptome v1.0, a glimpse in the brain transcriptome of five divergent Sauropsida lineages and the phylogenetic position of turtles.
abstract: BACKGROUND
  Reptiles are largely under-represented in comparative genomics despite the fact that they are substantially more diverse in many respects than mammals. Given the high divergence of reptiles from classical model species, next-generation sequencing of their transcriptomes is an approach of choice for gene identification and annotation.
  
  
  RESULTS
  Here, we use 454 technology to sequence the brain transcriptome of four divergent reptilian and one reference avian species: the Nile crocodile, the corn snake, the bearded dragon, the red-eared turtle, and the chicken. Using an in-house pipeline for recursive similarity searches of >3,000,000 reads against multiple databases from 7 reference vertebrates, we compile a reptilian comparative transcriptomics dataset, with homology assignment for 20,000 to 31,000 transcripts per species and a cumulated non-redundant sequence length of 248.6 Mbases. Our approach identifies the majority (87%) of chicken brain transcripts and about 50% of de novo assembled reptilian transcripts. In addition to 57,502 microsatellite loci, we identify thousands of SNP and indel polymorphisms for population genetic and linkage analyses. We also build very large multiple alignments for Sauropsida and mammals (two million residues per species) and perform extensive phylogenetic analyses suggesting that turtles are not basal living reptiles but are rather associated with Archosaurians, hence, potentially answering a long-standing question in the phylogeny of Amniotes.
  
  
  CONCLUSIONS
  The reptilian transcriptome (freely available at http://www.reptilian-transcriptomes.org) should prove a useful new resource as reptiles are becoming important new models for comparative genomics, ecology, and evolutionary developmental genetics.
authors.affiliation: Laboratory of Artificial & Natural Evolution (LANE), Dept, of Genetics & Evolution, University of Geneva, Sciences III, 30, Quai Ernest-Ansermet, 1211 Genève 4, Switzerland. michel.milinkovitch@unige.ch.
authors.forename: Athanasia C
authors.initials: AC
authors.lastname: Tzika
authors.forename: Raphaël
authors.initials: R
authors.lastname: Helaers
authors.forename: Gerrit
authors.initials: G
authors.lastname: Schramm
authors.forename: Michel C
authors.initials: MC
authors.lastname: Milinkovitch