title: [Optimization of culture conditions for production of extracellular polysaccharides by Morchella esculenta].
abstract: OBJECTIVE
  To optimize the conditions for exopolysaccharides (EPS) production by Morchella esculenta GIM 5.69.
  
  
  METHODS
  A Plackett-Burman design was used to evaluate the effects of variables factors. The optimal combined conditions for maximum polysaccharide production were further optimized by response surface methodology.
  
  
  RESULTS
  The optimal fermentation conditions were determined as follows: glucose 30%, cultivation time 5.98 d, rotary speed 217.44 r/min. The average yield of polysaccharide validation experiments was 53.04%, the relative error was 1.64%.
  
  
  CONCLUSIONS
  This research has the vital significance regarding the Morchella esculenta polysaccharide function of the product development and the application.
authors.affiliation: School of Life Science, Anhui University, Hefei 230039, China. huali8782@126.com
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Cui
authors.forename: Zeng-Wu
authors.initials: ZW
authors.lastname: Liu
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Fang
authors.forename: Gui-Qing
authors.initials: GQ
authors.lastname: Kai
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000363:Agaricales / Q000254:growth & development / Q000378:metabolism*; D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques / Q000379:methods*; D005285:Fermentation; D008954:Models, Biological; D011134:Polysaccharides / Q000096:biosynthesis* / Q000302:isolation & purification; D012044:Regression Analysis; D013395:Sucrose; D013997:Time Factors
chemical_list: D003470:Culture Media; D011134:Polysaccharides; D013395:Sucrose