title: Up-regulation of 42 kDa tubulin alpha-6 chain fragment in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus.
abstract: We performed proteomic differential display analysis of hepatitis C virus-associated 21 human hepatocellular carcinoma (HCV-HCC) tissues by using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). One of the numerous spots which was located next to three spots of glutamine synthetase showed stronger intensity in well-differentiated HCC tissues compared to non-cancerous tissues. Samples from 6 out of 21 patients showed up-regulation of this spot compared to non-cancerous tissues. After in-gel digestion, MALDITOF/MS identified the spot as tubulin alpha-6 chain. Two-dimensional immunoblot analysis confirmed that this spot was indeed tubulin alpha, and this spot was stronger in cancerous tissues than in noncancerous tissues. These results suggest that tubulin alpha-6 chain is one of the candidates for biomarkers for well-differentiated HCV-associated HCC.
authors.affiliation: Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan. climates@yamaguchi-u.ac.jp
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Motonari
authors.initials: M
authors.lastname: Takashima
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Yokoyama
authors.forename: Norio
authors.initials: N
authors.lastname: Iizuka
authors.forename: Takao
authors.initials: T
authors.lastname: Tamesa
authors.forename: Junko K
authors.initials: JK
authors.lastname: Akada
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Toshifusa
authors.initials: T
authors.lastname: Toda
authors.forename: Isao
authors.initials: I
authors.lastname: Sakaida
authors.forename: Kiwamu
authors.initials: K
authors.lastname: Okita
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000378:metabolism / Q000821:virology*; D002454:Cell Differentiation; D015180:Electrophoresis, Gel, Two-Dimensional; D016174:Hepacivirus / Q000502:physiology*; D006526:Hepatitis C / Q000150:complications / Q000378:metabolism / Q000821:virology*; D006801:Humans; D015151:Immunoblotting; D008113:Liver Neoplasms / Q000150:complications / Q000378:metabolism / Q000821:virology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D010446:Peptide Fragments; D014404:Tubulin