title: Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases.
abstract: A wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. Excretory and secretory proteins (ESP) are active at the interface between parasite and host and comprise potential targets for intervention. The intestinal nematode Strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living generations. We investigated ESP from infective larvae, parasitic females, and free-living stages of the rat parasite Strongyloides ratti, which is genetically very similar to the human pathogen, Strongyloides stercoralis. Proteomic analysis of ESP revealed 586 proteins, with the largest number of stage-specific ESP found in infective larvae (196), followed by parasitic females (79) and free-living stages (35). One hundred and forty proteins were identified in all studied stages, including anti-oxidative enzymes, heat shock proteins, and carbohydrate-binding proteins. The stage-selective ESP of (1) infective larvae included an astacin metalloproteinase, the L3 Nie antigen, and a fatty acid retinoid-binding protein; (2) parasitic females included a prolyl oligopeptidase (prolyl serine carboxypeptidase), small heat shock proteins, and a secreted acidic protein; (3) free-living stages included a lysozyme family member, a carbohydrate-hydrolyzing enzyme, and saponin-like protein. We verified the differential expression of selected genes encoding ESP by qRT-PCR. ELISA analysis revealed the recognition of ESP by antibodies of S. ratti-infected rats. A prolyl oligopeptidase was identified as abundant parasitic female-specific ESP, and the effect of pyrrolidine-based prolyl oligopeptidase inhibitors showed concentration- and time-dependent inhibitory effects on female motility. The characterization of stage-related ESP from Strongyloides will help to further understand the interaction of this unique intestinal nematode with its host.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Hanns
authors.initials: H
authors.lastname: Soblik
authors.forename: Abuelhassan Elshazly
authors.initials: AE
authors.lastname: Younis
authors.forename: Makedonka
authors.initials: M
authors.lastname: Mitreva
authors.forename: Bernhard Y
authors.initials: BY
authors.lastname: Renard
authors.forename: Marc
authors.initials: M
authors.lastname: Kirchner
authors.forename: Frank
authors.initials: F
authors.lastname: Geisinger
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.forename: Norbert W
authors.initials: NW
authors.lastname: Brattig
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003470:Culture Media / Q000737:chemistry; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D007106:Immune Sera / Q000737:chemistry; D007422:Intestines / Q000469:parasitology; D007814:Larva / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D008297:Male; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D000085822:Prolyl Oligopeptidases; D011480:Protease Inhibitors / Q000494:pharmacology; D021382:Protein Sorting Signals; D017434:Protein Structure, Tertiary; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D018709:Statistics, Nonparametric; D017170:Strongyloides ratti / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013322:Strongyloidiasis / Q000469:parasitology
chemical_list: D003470:Culture Media; D015801:Helminth Proteins; D007106:Immune Sera; D011480:Protease Inhibitors; D021382:Protein Sorting Signals; D010447:Peptide Hydrolases; D012697:Serine Endopeptidases; C000705287:PREPL protein, human; D000085822:Prolyl Oligopeptidases