title: Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze.
abstract: OBJECTIVE
  To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP)  MATERIALS AND METHODS: One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt free radical assays were established to measure the antioxidant capacity of the LSEP in vitro.
  
  
  RESULTS
  According to analysis, extraction temperature significantly affected extraction yield. The optimum extraction conditions for LSEP were as follows: extraction temperature, 95°C; ultrasonic time 50 minutes; and dosage liquor ratio, 1: 12. Under these conditions, the experimental yield of crude LSEP was 12.80±0.19% which was well matched with the predicted models. The antioxidant capacity data suggested that LSEP has strong antioxidant activity.
  
  
  CONCLUSIONS
  One-singer factor and RSM were used to extract of LSEP are simple and feasible and LSEP could be developed as a nutraceutical agent for itsstrong antioxidant activity.
authors.affiliation: Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers University, Yancheng - 224002.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Tang
authors.forename: Lifang
authors.initials: L
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ge
authors.forename: Haidong
authors.initials: H
authors.lastname: Yang
authors.forename: Na
authors.initials: N
authors.lastname: Lin