title: Colorimetric assay for the quantification of serum iodixanol concentration and its application on estimation of glomerular filtration rate in cattle.
abstract: A colorimetric assay used to quantify the non-ionic contrast medium iodixanol in sera was validated and compared with high-performance liquid chromatography. The application of this assay to estimate glomerular filtration rates (GFR) in cattle was examined. Serum iodixanol was de-iodinated by alkaline hydrolysis and the amount of released iodine was subsequently determined using a ceric arsenite method. There was a close correlation between the two methods using identical specimens. In clinically healthy cattle with different body weights, the reference value (166.3-178.8 mL/min/m(2)) based on body surface area was fairly stable as compared with that (2.13-3.63 mL/min/kg) based on body weight. Based on GFR data in healthy and renal-impaired cattle, when the GFR decreased to more than 60% of the reference value, serum urea nitrogen and creatinine concentrations increased. The colorimetric assay is a simple method for the estimation of GFR in cattle and requires no expensive equipment.
authors.affiliation: Department of Veterinary Clinical Medicine, Iwate University, Morioka 020-8550, Japan.
authors.forename: Moe
authors.initials: M
authors.lastname: Miura
authors.forename: Norio
authors.initials: N
authors.lastname: Yamagishi
authors.forename: Kouya
authors.initials: K
authors.lastname: Sasaki
authors.forename: Danil
authors.initials: D
authors.lastname: Kim
authors.forename: Bhuminand
authors.initials: B
authors.lastname: Devkota
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D003124:Colorimetry / Q000379:methods / Q000662:veterinary*; D003287:Contrast Media / Q000032:analysis*; D005260:Female; D005919:Glomerular Filtration Rate / Q000502:physiology*; D008297:Male; D014283:Triiodobenzoic Acids / Q000097:blood*
chemical_list: D003287:Contrast Media; D014283:Triiodobenzoic Acids; C044834:iodixanol