title: Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster.
abstract: Two genes from the "mycobacterial arabinogalactan biosynthetic cluster" spanning the region from Rv3779 to Rv3809c in the genome of Mycobacterium tuberculosis H37Rv were annotated as possible components of the ATP-binding cassette transporter. Rv3781 encodes a nucleotide-binding domain and Rv3783 determines production of a membrane-spanning domain. We have examined possible roles of these genes in mycobacterial cell wall biosynthesis through inactivation of their respective orthologs in Mycobacterium smegmatis mc(2)155, phenotypic characterization of the mutant strains via metabolic labeling with [U-(14)C]-glucose, cell-free reactions with UDP-[U-(14)C]-galactose monitoring galactan build-up and transcriptional analysis. Several lines of evidence suggest that this ABC transporter is involved in biosynthesis of arabinogalactan, although more investigation is needed to establish its precise role or the transported substrate.
authors.affiliation: Department of Biochemistry, Comenius University, Bratislava, Slovakia. mikusova@fns.uniba.sk
authors.forename: Petronela
authors.initials: P
authors.lastname: Dianišková
authors.forename: Jana
authors.initials: J
authors.lastname: Korduláková
authors.forename: Henrieta
authors.initials: H
authors.lastname: Skovierová
authors.forename: Devinder
authors.initials: D
authors.lastname: Kaur
authors.forename: Mary
authors.initials: M
authors.lastname: Jackson
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Brennan
authors.forename: Katarína
authors.initials: K
authors.lastname: Mikušová
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000737:chemistry; D002473:Cell Wall / Q000737:chemistry; D002474:Cell-Free System; D005685:Galactans / Q000737:chemistry*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016680:Genome, Bacterial; D005947:Glucose / Q000737:chemistry; D008954:Models, Biological; D008957:Models, Genetic; D009154:Mutation; D020102:Mycobacterium smegmatis / Q000378:metabolism*; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D018528:ATP-Binding Cassette Transporters; D005685:Galactans; D000255:Adenosine Triphosphate; D005947:Glucose; C005653:arabinogalactan