title: Surface modification of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer films for promoting interaction with bladder urothelial cells.
abstract: Often bladder dysfunction and diseases lead to therapeutic interventions that require partial or complete replacement of damaged tissue. For this reason, the development of biomaterials to repair the bladder by promoting the adhesion and growth of urothelial cells is of interest. With this aim, a modified copolyester of biocompatible and biodegradable poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(HB-co-HV)] was used as scaffold for porcine urothelial cell culture. In addition to good biocompatibility, the surface of P(HB-co-HV) substrates was modified to provide both, higher hydrophilicity and a better interaction with urothelial cells. Chemical treatments with ethylenediamine (ED) and sodium hydroxide (NaOH) led to substrate surfaces with decreasing hydrophobicity and provided functional groups that enable the grafting of bioactive molecules, such as a laminin derived YIGSR sequence. Physico-chemical properties of modified substrates were studied and compared with those of the pristine P(HB-co-HV). Urothelial cell morphology on treated substrates was studied. The results showed that focal attachment and cell-related properties were improved for peptide grafted polymer compared with both, the unmodified and functionalized copolyester.
authors.affiliation: Departamento de Química-Física de Polímeros, Instituto de Ciencia y Tecnología de Polímeros, Consejo Superior de Investigaciones Científicas-ICTP-CSIC, Juan de la Cierva 3, E-28006 Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: García-García
authors.forename: Laura
authors.initials: L
authors.lastname: López
authors.forename: Rodrigo
authors.initials: R
authors.lastname: París
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Núñez-López
authors.forename: Isabel
authors.initials: I
authors.lastname: Quijada-Garrido
authors.forename: Enrique
authors.initials: E
authors.lastname: de la Peña Zarzuelo
authors.forename: Leoncio
authors.initials: L
authors.lastname: Garrido
mesh_terms: D000588:Amines / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D005029:Ethylenediamines / Q000737:chemistry; D006868:Hydrolysis / Q000187:drug effects; D007797:Laminin / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology*; D012972:Sodium Hydroxide / Q000737:chemistry; D012996:Solutions; D013499:Surface Properties / Q000187:drug effects; D034421:Sus scrofa; D013997:Time Factors; D001743:Urinary Bladder / Q000166:cytology*; D019459:Urothelium / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D000094122:Polyhydroxybutyrates
chemical_list: D000588:Amines; D005029:Ethylenediamines; D007797:Laminin; D011091:Polyesters; D012972:Sodium Hydroxide; D012996:Solutions; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C031234:ethylenediamine