title: Unexpected properties of endostatin-producing mouse BCR-ABL-transformed             cells.
abstract: We investigated whether a genetic modification of BCR-ABL-transformed mouse             cells that resulted in endostatin (ES) production altered their oncogenic potential.             Mouse B210 cells, which express p210bcr-abl fusion protein and induce leukemia-like             disease and extremely rarely solid tumors after intravenous (i.v.) administration,             were used. The cells were transfected with a plasmid carrying genes for mouse             ES and resistance to blasticidine. Transduced cells were isolated in media supplemented             with blasticidine. Production of ES was determined by Western blotting. For further             tests, two clones were selected, and their pathogenicity after i.v. inoculation             was tested. Compared with the parental B210 cells, the capability of both gene-modified             cell clones to induce lethal leukemia was reduced. However, mice that did not             succumb to leukemia subsequently developed solid tumors. They were composed of             poorly differentiated cells with irregular nuclei and roughly granular chromatin             and were well vascularized. FISH revealed the presence of the BCR-ABL fusion gene             both in tumors and spleens. Immunohistological investigation of the tumors demonstrated             the production of ES in vivo and the cell lines derived from the tumors produced             detectable amounts of ES, this demonstrating that the formation of solid tumors             was not associated with the loss or silencing of the ES gene.
authors.affiliation: Department of Experimental Virology, Institute of Hematology                     and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Krmenčíková
authors.forename: Libor
authors.initials: L
authors.lastname: Staněk
authors.forename: Martina
authors.initials: M
authors.lastname: Petráčková
authors.forename: Pavel
authors.initials: P
authors.lastname: Dundr
authors.forename: Vladimír
authors.initials: V
authors.lastname: Vonka
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D017077:Culture Media, Conditioned / Q000494:pharmacology; D043169:Endostatins / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics* / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D053208:Kaplan-Meier Estimate; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation
chemical_list: D017077:Culture Media, Conditioned; D043169:Endostatins; D015395:Histocompatibility Antigens Class I; D016044:Fusion Proteins, bcr-abl