title: Enzymes acting on D-amino acid containing peptides.
abstract: Using a synthetic oligopeptide (D-Phe)(4), a microorganism Bacillus cereus DF4-B producing alkaline D-peptidase (ADP) was isolated. The enzymatic properties have been characterized; the enzyme showed D-stereospecific dipeptidyl aminopeptidase and endopeptidase activities. The enzyme was active toward (D-Phe)(n), Boc-(D-Phe)(n), (D-Phe)(n) methyl ester, D-Phe-NH(2), Boc-(D-Phe)(n) methyl ester, and Boc-(D-Phe)(n) tert-butyl ester, but not toward (D-Ala)(n) (n = 2-4), (D-Val)(3), and (D-Leu)(2).
authors.affiliation: Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan. asano@pu-toyama.ac.jp
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Asano
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000626:Aminopeptidases / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D010455:Peptides; D010450:Endopeptidases; D000626:Aminopeptidases