title: In vitro evaluation of glass-glass ceramic thermoseed-induced hyperthermia on human osteosarcoma cell line.
abstract: The use of biomaterials as implantable thermoseeds under the action of an external magnetic field is a very interesting methodology to focus the heat into the target tumors as osteosarcoma. In this study, biocompatible and bioactive G15GC85 thermoseeds, tailored through the combination of sol-gel glasses (G) with a magnetic glass ceramic (GC), were used to induce hyperthermia on cultured human osteosarcoma cells after exposition to alternating magnetic field (MF, 100 kHz/200 Oe). G15GC85 magnetic glass-glass ceramic thermoseeds induced in vitro effective hyperthermia with drastic reduction in proliferation of human osteosarcoma Saos-2 cells and high increase of apoptotic cells after two 40 min consecutive sessions of MF. Deep cell morphology alterations were observed after this hyperthermic treatment, and the proteomic analysis revealed modification of gamma actin molecular properties related to cytoskeleton alterations. These results indicate that G15GC85 thermoseeds allow to induce in vitro effective hyperthermia on human osteosarcoma cells.
authors.affiliation: Biochemistry and Molecular Biology I, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Alcaide
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ramírez-Santillán
authors.forename: María José
authors.initials: MJ
authors.lastname: Feito
authors.forename: María de la Concepción
authors.initials: Mde L
authors.lastname: Matesanz
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ruiz-Hernández
authors.forename: Daniel
authors.initials: D
authors.lastname: Arcos
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
authors.forename: María Teresa
authors.initials: MT
authors.lastname: Portolés
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D042541:Intracellular Space / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008970:Molecular Weight; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D012516:Osteosarcoma / Q000628:therapy*; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013696:Temperature
chemical_list: D003470:Culture Media; D017382:Reactive Oxygen Species; C023506:Glass ceramics; D007770:L-Lactate Dehydrogenase