title: [Effect of phosphatidylinositol 3-kinase/serine-threonine kinase signalling pathway during the proliferation process of HL-60 cells exposed to benzoquinone].
abstract: OBJECTIVE
  To explore the effects of phosphatidylinositol 3-kinase/Serine-threonine kinase (PI3K/Akt) signal pathway on the proliferation of HL-60 cells exposed to benzoquinone (BQ).
  
  
  METHODS
  HL60 cells were divided into 3 groups: control group (treated with PBS), BQ group (treated with 3 micromol/L BQ) and LY294002 plus BQ group (treated with 20 micromol/L LY294002 plus 3 micromol/L BQ). The cell proliferation was measured with alamar blue dye assay. Western blot assay was used to detect the expression of p-Akt and Akt proteins and flow cytometer was used to observe the cell cycle.
  
  
  RESULTS
  The cell proliferation rate and the cell proportion in the S, G2 phase of BQ group were 185.00% +/- 30.00%, 48.23% +/- 1.37% and 15.40% +/- 1.21%, respectively, which were significantly higher than those (100.00% +/- 0.00%, 42.47% +/- 0.45% and 5.40% +/- 0.40%) of control group (P<0.05). But the cell proportion rate (36.37% +/- 0.40%) in the G1 phase in BQ group was significantly lower than that (52.13 +/- 0.75%) in control group (P<0.05). The expression level of p-Akt protein in BQ group was significantly higher than that in control group (P<0.05). The cell proliferation rate and the cell proportion in the S, G2 phase of LY294002 plus BQ group were 82.59% +/- 15.00%, 42.03% +/- 0.50% and 3.87% +/- 0.47%, respectively, which were significantly lower than those of BQ group (P<0.05). But the cell proportion rate (54.43% +/- 0.40%) in the G1 phase in LY294002 plus BQ group was significantly higher than that in BQ group (P<0.05).
  
  
  CONCLUSIONS
  The PI3K/Akt signal pathway may play an important role in the proliferation of HL-60 cells exposed to BQ.
authors.affiliation: Guangzhou Occupational Disease Prevention & Treatment Center, Guangzhou 510620, China.
authors.forename: Yi-min
authors.initials: YM
authors.lastname: Liu
authors.forename: Yong-sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Xu-dong
authors.initials: XD
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Guo
authors.forename: Chun-hui
authors.initials: CH
authors.lastname: Gu
mesh_terms: D016227:Benzoquinones / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D016227:Benzoquinones; C004532:quinone; D058539:Phosphatidylinositol 3-Kinase; D017346:Protein Serine-Threonine Kinases