title: Chondrocyte culture and assay.
abstract: Chondrocytes constitute the sole cell type found within cartilage, and control the formation and composition of cartilage. Cellular, biochemical and pharmacological studies of arthritis and other cartilage disorders have increasingly focused on chondrocyte function. Three methods are presented in this unit for culturing chondrocytes, and two assays are described that characterize proteoglycan synthesis, a key measure of chondrocyte function.Chondrocytes constitute the sole cell type found within cartilage, and control the formation and composition of cartilage.
authors.affiliation: Novartis Institute of Biomedical Research, Summit, New Jersey, USA.
authors.forename: J
authors.initials: J
authors.lastname: Liebman
authors.forename: R L
authors.initials: RL
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology*; D011509:Proteoglycans / Q000096:biosynthesis
chemical_list: D011509:Proteoglycans