title: AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in Arabidopsis.
abstract: Various transcription factors are involved in the response to environmental stresses in plants. In this study, we characterized AtERF71/HRE2, a member of the Arabidopsis AP2/ERF family, as an important regulator of the osmotic and hypoxic stress responses in plants. Transcript level of AtERF71/HRE2 was highly increased by anoxia, NaCl, mannitol, ABA, and MV treatments. aterf71/hre2 loss-of-function mutants displayed higher sensitivity to osmotic stress such as high salt and mannitol, accumulating higher levels of ROS under high salt treatment. In contrast, AtERF71/HRE2-overexpressing transgenic plants showed tolerance to salt and mannitol as well as flooding and MV stresses, exhibiting lower levels of ROS under high salt treatment. AtERF71/HRE2 protein was localized in the nucleus, and the C-terminal region of AtERF71/HRE2 was required for transcription activation activity. Taken together, our results suggest that AtERF71/HRE2 might function as a transcription factor involved in the response to osmotic stress as well as hypoxia.
authors.affiliation: Department of Molecular Biology, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Hee-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Hye-Yeon
authors.initials: HY
authors.lastname: Seok
authors.forename: Dong-Hyuk
authors.initials: DH
authors.lastname: Woo
authors.forename: Sun-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Vaishali N
authors.initials: VN
authors.lastname: Tarte
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Lee
authors.forename: Choon-Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Moon
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009995:Osmosis; D009997:Osmotic Pressure; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D008353:Mannitol; D012965:Sodium Chloride; D014157:Transcription Factors; C568479:AT2G47520 protein, Arabidopsis