title: Bag3 promotes resistance to apoptosis through Bcl-2 family members in             non-small cell lung cancer.
abstract: In non-small cell lung cancer (NSCLC) certain molecular characteristics,             which are related to molecular alterations have been investigated. These are responsible             for both the initiation and maintenance of the malignancy in lung cancer. The             aim of this study was to evaluate the influence of Bag3 (Bcl-2 associated athanogene             3) in the regulation of apoptosis on NSCLC. Bag3 and Hsp70 expression were examined             by immunohistochemistry to confirm their potential roles in the prevalence of             NSCLC. We also established human normal bronchial epithelial cells and HOP-62             cell line as the model to analyze cell apoptosis and the expression of Hsp70,             Bcl-XL and Bcl-2, which were affected by Bag3. In this study, we found that Bag3             and Hsp70 are highly expressed in few tissues and cell lines of NSCLC. Bag3 inhibits             apoptosis in human normal bronchial epithelial cell lines and sustain the survival             of NSCLC cells. Bag3, Hsp70, Bcl-XL and Bcl-2 are up-regulated in NSCLC cell lines.             At the same time, the silencing of Bag3 results in diminishing protein levels             of Bcl-XL and Bcl-2. The results of immunoprecipitation identified that Bag3 could             interact with Hsp70, Bcl-XL and Bcl-2 NSCLC cells directly or indirectly. We conclude             that NSCLC cells were protected from apoptosis through increasing Bag3 expression             and consequently promoted the expression of Bcl-XL and Bcl-2.
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lu
authors.forename: Yun-Jie
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor / Q000032:analysis; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D051020:bcl-X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C414431:BAG3 protein, human; D014408:Biomarkers, Tumor; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein