title: Bioactive compounds extracted from Indian wild legume seeds: antioxidant and type II diabetes-related enzyme inhibition properties.
abstract: Seven different wild legume seeds (Acacia leucophloea, Bauhinia variegata, Canavalia gladiata, Entada scandens, Mucuna pruriens, Sesbania bispinosa and Tamarindus indica) from various parts of India were analyzed for total free phenolics, l-Dopa (l-3,4 dihydroxyphenylalanine), phytic acid and their antioxidant capacity (ferric-reducing antioxidant power [FRAP] and 2,2-diphenyl-1-picrylhydrazyl [DPPH] assay) and type II diabetes-related enzyme inhibition activitiy (α-amylase). S. bispinosa had the highest content in both total free phenolics and l-Dopa, and relatively low phytic acid when compared with other seeds. Phytic acid content, being highest in E. scandens, M. pruriens and T. indica, was highly predictive for FRAP (r = 0.47, p < 0.05) and DPPH (r = 0.66, p < 0.001) assays. The phenolic extract from T. indica and l-Dopa extract from E. scandens showed significantly higher FRAP values among others. All seed extracts demonstrated a remarkable reducing power (7-145 mM FeSO4 per mg extract), DPPH radical scavenging activity (16-95%) and α-amylase enzyme inhibition activity (28-40%).
authors.affiliation: Institute of Biological Chemistry and Nutrition, University of Hohenheim, Stuttgart, Germany. gautambazz@googlemail.com
authors.forename: Basanta
authors.initials: B
authors.lastname: Gautam
authors.forename: Vellingiri
authors.initials: V
authors.lastname: Vadivel
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Stuetz
authors.forename: Hans K
authors.initials: HK
authors.lastname: Biesalski
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D007194:India; D007980:Levodopa / Q000032:analysis / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D010833:Phytic Acid / Q000032:analysis; D008517:Phytotherapy; D010851:Picrates / Q000378:metabolism; D012639:Seeds; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004791:Enzyme Inhibitors; D010636:Phenols; D010851:Picrates; D007980:Levodopa; D010833:Phytic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000516:alpha-Amylases