title: Freshwater mudsnail (Potamopyrgus antipodarum) estrogen receptor: identification and expression analysis under exposure to (xeno-)hormones.
abstract: Molluscs are raising attention as ecotoxicological test organisms due to their high diversity and ecological importance. The ovoviviparous prosobranch gastropod Potamopyrgus antipodarum (freshwater mudsnail) responds very sensitively to xenobiotics and has therefore been proposed as OECD standard test organism. Endocrine disrupting chemicals influence the reproduction of P. antipodarum, which can be assessed by embryo numbers in the brood pouch. However, the knowledge about the endocrine system of P. antipodarum is rather limited. The aim of this study was to identify an estrogen receptor in the endocrine system of P. antipodarum and to investigate if this receptor is differentially expressed under exposure to (xeno-)hormones (17α-ethinylestradiol, bisphenol A and 17α-methyltestosterone). The DNA-binding domain of the identified ER-like transcript has an amino acid identity of 92 percent compared to the ER of the gastropod Nucella lapillus (84 percent to human ERα) and 83 percent in the ligand binding domain (38 percent to human ERα). Furthermore, the P. antipodarum ER is transcriptionally regulated as shown by quantitative real-time PCRs of (xeno-)hormone exposed snails. 17α-ethinylestradiol and bisphenol A exposure resulted in a transitory ER-mRNA increase while17α-methyltestosterone caused a transitory reduction of ER-mRNA. In addition the solvent dimethyl sulfoxide had also a modulating effect on the receptor.
authors.affiliation: Department Aquatic Ecotoxicology, Institute for Ecology, Evolution and Diversity, Goethe University, Frankfurt, Germany. stange@bio.uni-frankfurt.de
authors.forename: Daniela
authors.initials: D
authors.lastname: Stange
authors.forename: Agnes
authors.initials: A
authors.lastname: Sieratowicz
authors.forename: Ralf
authors.initials: R
authors.lastname: Horres
authors.forename: Jörg
authors.initials: J
authors.lastname: Oehlmann
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors / Q000633:toxicity; D004703:Endocrine System / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology; D004968:Estrogens, Non-Steroidal / Q000633:toxicity; D004997:Ethinyl Estradiol / Q000633:toxicity; D005618:Fresh Water / Q000737:chemistry; D006728:Hormones / Q000633:toxicity*; D006801:Humans; D008777:Methyltestosterone / Q000633:toxicity; D010636:Phenols / Q000633:toxicity; D011960:Receptors, Estrogen / Q000378:metabolism*; D012098:Reproduction / Q000187:drug effects; D012908:Snails / Q000187:drug effects / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004967:Estrogens; D004968:Estrogens, Non-Steroidal; D004997:Ethinyl Estradiol; D006728:Hormones; D008777:Methyltestosterone; D010636:Phenols; D011960:Receptors, Estrogen; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; C006780:bisphenol A; D000098826:Bisphenol A Compounds