title: Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation.
abstract: Pseudouridines are the most abundant and highly conserved modified nucleotides identified in spliceosomal small nuclear RNAs (snRNAs). Most pseudouridines are also clustered in functionally important regions of spliceosomal snRNAs. Experiments carried out in several independent experimental systems show that the pseudouridines in spliceosomal snRNAs are functionally important for pre-messenger RNA (mRNA) splicing. Experimental data also indicate that spliceosomal snRNA pseudouridylation can be catalyzed by both RNA-dependent (box H/ACA Ribonucleoproteins) and RNA-independent (protein-only enzymes) mechanisms.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
authors.forename: Guowei
authors.initials: G
authors.lastname: Wu
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Yu
authors.forename: Athena
authors.initials: A
authors.lastname: Kantartzis
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D006801:Humans; D008958:Models, Molecular; D058977:Molecular Sequence Annotation; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000378:metabolism; D011560:Pseudouridine / Q000737:chemistry / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism*; D014981:Xenopus
chemical_list: D012331:RNA, Fungal; D012342:RNA, Small Nuclear; D011560:Pseudouridine