title: Chlamydia trachomatis inclusions induce asymmetric cleavage furrow formation and ingression failure in host cells.
abstract: Chlamydia trachomatis infection has been suggested to induce host genome duplication and is linked to increased risks of cervical cancer. We describe here the mechanism by which Chlamydia causes a cleavage furrow defect that consistently results in the formation of multinucleated host cells, a phenomenon linked to tumorigenesis. Host signaling proteins essential for cleavage furrow initiation, ingression, and stabilization are displaced from one of the prospective furrowing cortices after Chlamydia infection. This protein displacement leads to the formation of a unique asymmetrical, unilateral cleavage furrow in infected human cells. The asymmetrical distribution of signaling proteins is caused by the physical presence of the Chlamydia inclusion at the cell equator. By using ingested latex beads, we demonstrate that the presence of a large vacuole at the cell equator is sufficient to cause furrow ingression failure and can lead to multinucleation. Interestingly, internalized latex beads of similar size do not localize to the cell equator as efficiently as Chlamydia inclusions; moreover, inhibition of bacterial protein synthesis with antibiotic reduces the frequency at which Chlamydia localizes to the cell equator. Together, these results suggest that Chlamydia effectors are involved in strategic positioning of the inclusion during cell division.
authors.affiliation: Department of Cell and Systems Biology, Department of Biological Sciences, University of Toronto at Scarborough, Toronto, Ontario M1C 1A4, Canada.
authors.forename: He Song
authors.initials: HS
authors.lastname: Sun
authors.forename: Andrew
authors.initials: A
authors.lastname: Wilde
authors.forename: Rene E
authors.initials: RE
authors.lastname: Harrison
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002455:Cell Division; D002690:Chlamydia Infections / Q000382:microbiology / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008938:Mitosis; D010587:Phagocytosis; D015398:Signal Transduction; D008941:Spindle Apparatus / Q000378:metabolism; D013692:Telophase; D014617:Vacuoles / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins