title: Growth of the oleaginous microalga Aurantiochytrium sp. KRS101 on cellulosic biomass and the production of lipids containing high levels of docosahexaenoic acid.
abstract: We examined the growth of a novel oleaginous microalga, Aurantiochytrium sp. KRS101, using cellulosic materials as nutrients, and the resultant production of lipids containing high levels of docosahexaenoic acid (DHA). The microalgal strain could grow using either carboxymethylcellulose or cellobiose as a carbon source, and produced lipids containing high levels of DHA (49-58% of total fatty acids). In line with this growth behavior, carboxymethylcellulase and cellobiohydrolase activities were evident in both cell-free lysates and culture broths. Additionally, an industrial cellulosic biomass, palm oil empty fruit bunches (POEFB), a by-product of the palm oil industry, were utilized by the microalgal strain for cell growth and lipid production.
authors.affiliation: Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea.
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Dina
authors.initials: D
authors.lastname: Rairakhwada
authors.forename: Seonghun
authors.initials: S
authors.lastname: Kim
authors.forename: Byung-Ki
authors.initials: BK
authors.lastname: Hur
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
mesh_terms: D028023:Arecaceae / Q000382:microbiology*; D049109:Cell Proliferation; D002482:Cellulose / Q000378:metabolism*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis* / Q000737:chemistry; D005638:Fruit / Q000382:microbiology*; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D013045:Species Specificity; D058009:Stramenopiles / Q000145:classification / Q000502:physiology*
chemical_list: D008055:Lipids; D004281:Docosahexaenoic Acids; D002482:Cellulose