title: Antioxidative activity of propolis extract in yeast cells.
abstract: The antioxidative activities of propolis and its main phenolic compounds, caffeic acid, p-coumaric acid, ferulic acid, and caffeic acid phenethyl ester, were investigated in the yeast Saccharomyces cerevisiae. After 1 h of exposure of the yeast cells, their intracellular oxidation was measured using 2',7'-dichlorofluorescein. Yeast cells exposed to 96% ethanolic extracts of propolis in DMSO (EEP) showed decreased intracellular oxidation, with no significant differences seen for the individual phenolic compounds. However, cellular uptake was seen only for a moderately polar fraction of EEP (E2) and caffeic acid phenethyl ester. The EEP antioxidative activity thus resulted from this E2 fraction of EEP. The influence of EEP was also investigated at the mitochondrial proteome level, by analyzing its profile after 1 h of exposure of the yeast cells to EEP and E2. Changes in the levels of antioxidative proteins and proteins involved in ATP synthesis were seen.
authors.affiliation: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Cigut
authors.forename: Tomaž
authors.initials: T
authors.lastname: Polak
authors.forename: Lea
authors.initials: L
authors.lastname: Gašperlin
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
authors.forename: Polona
authors.initials: P
authors.lastname: Jamnik
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011429:Propolis / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D029701:Saccharomyces cerevisiae Proteins; D011429:Propolis