title: Fermentation strategies for 1,3-propanediol production from glycerol using a genetically engineered Klebsiella pneumoniae strain to eliminate by-product formation.
abstract: We generated a genetically engineered Klebsiella pneumoniae strain (AK-VOT) to eliminate by-product formation during production of 1,3-propanediol (1,3-PD) from glycerol. In the present study, the glycerol-metabolizing properties of the recombinant strain were examined during fermentation in a 5 L bioreactor. As expected, by-product formation was completely absent (except for acetate) when the AK-VOT strain fermented glycerol. However, 1,3-PD productivity was severely reduced owing to a delay in cell growth attributable to a low rate of glycerol consumption. This problem was solved by establishing a two-stage process separating cell growth from 1,3-PD production. In addition, nutrient co-supplementation, especially with starch, significantly increased 1,3-PD production from glycerol during fed-batch fermentation by AK-VOT in the absence of by-product formation.
authors.affiliation: Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, Korea.
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Sun-Yeon
authors.initials: SY
authors.lastname: Heo
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Lian Hua
authors.initials: LH
authors.lastname: Luo
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Son
authors.forename: Don-Hee
authors.initials: DH
authors.lastname: Park
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000085:Acetates / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D005990:Glycerol / Q000302:isolation & purification / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000502:physiology*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D000085:Acetates; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol