title: [Metabolic footprint in conditioned culture medium of placental explants: a comparison between early-onset and late-onset severe preeclampsia].
abstract: OBJECTIVE
  To explore the differences of metabolic footprint in the conditioned culture medium of placental explants between early-onset and late-onset severe preeclampsia.
  
  
  METHODS
  In 13 cases of early-onset severe preeclampsia and 14 cases of late-onset severe preeclampsia, the placentas were sampled at the surface of the maternal placenta. High performance liquid chromatography-mass spectrometry (HPLC-MS) was used to determine the differences in the metabolites in the conditioned culture medium of the placental villous explants cultured in 6% atmospheric O(2) for 96 h. Standard samples were used to establish the tryptophan and kynurenine chromatography library by HPLC-MS to analyze the concentration of tryptophan and kynurenine in the conditioned culture medium.
  
  
  RESULTS
  Thirty-six metabolites showed statistically significant differences between early-onset and late-onset severe preeclampsia (P<0.05). The concentration of kynurenine was significantly higher in early-onset severe preeclampsia than in late-onset severe preeclampsia (P<0.05).
  
  
  CONCLUSIONS
  Early-onset and late-onset severe preeclampsia may have different pathogeneses. By detecting the concentration of metabolites, metabolomic strategies provide a new means for predicting the onset time of severe preeclampsia.
authors.affiliation: Southern Medical University, Guangzhou, China. pinguolv@126.com
authors.forename: Da-yan
authors.initials: DY
authors.lastname: Liu
authors.forename: Shi-ling
authors.initials: SL
authors.lastname: Chen
authors.forename: Chen-hong
authors.initials: CH
authors.lastname: Wang
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Luo
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Huang
mesh_terms: D002824:Chorionic Villi / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000737:chemistry*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007737:Kynurenine / Q000378:metabolism; D009952:Ornithine / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D014364:Tryptophan / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D007737:Kynurenine; D014364:Tryptophan; D009952:Ornithine