title: Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis.
abstract: The heterotrimeric G-protein complex is minimally composed of Gα, Gβ, and Gγ subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.
authors.affiliation: Botanical Institute, University of Cologne, Cologne, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Klopffleisch
authors.forename: Nguyen
authors.initials: N
authors.lastname: Phan
authors.forename: Kelsey
authors.initials: K
authors.lastname: Augustin
authors.forename: Robert S
authors.initials: RS
authors.lastname: Bayne
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Booker
authors.forename: Jose R
authors.initials: JR
authors.lastname: Botella
authors.forename: Nicholas C
authors.initials: NC
authors.lastname: Carpita
authors.forename: Tyrell
authors.initials: T
authors.lastname: Carr
authors.forename: Jin-Gui
authors.initials: JG
authors.lastname: Chen
authors.forename: Thomas Ryan
authors.initials: TR
authors.lastname: Cooke
authors.forename: Arwen
authors.initials: A
authors.lastname: Frick-Cheng
authors.forename: Erin J
authors.initials: EJ
authors.lastname: Friedman
authors.forename: Brandon
authors.initials: B
authors.lastname: Fulk
authors.forename: Michael G
authors.initials: MG
authors.lastname: Hahn
authors.forename: Kun
authors.initials: K
authors.lastname: Jiang
authors.forename: Lucia
authors.initials: L
authors.lastname: Jorda
authors.forename: Lydia
authors.initials: L
authors.lastname: Kruppe
authors.forename: Chenggang
authors.initials: C
authors.lastname: Liu
authors.forename: Justine
authors.initials: J
authors.lastname: Lorek
authors.forename: Maureen C
authors.initials: MC
authors.lastname: McCann
authors.forename: Antonio
authors.initials: A
authors.lastname: Molina
authors.forename: Etsuko N
authors.initials: EN
authors.lastname: Moriyama
authors.forename: M Shahid
authors.initials: MS
authors.lastname: Mukhtar
authors.forename: Yashwanti
authors.initials: Y
authors.lastname: Mudgil
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Pattathil
authors.forename: John
authors.initials: J
authors.lastname: Schwarz
authors.forename: Steven
authors.initials: S
authors.lastname: Seta
authors.forename: Matthew
authors.initials: M
authors.lastname: Tan
authors.forename: Ulrike
authors.initials: U
authors.lastname: Temp
authors.forename: Yuri
authors.initials: Y
authors.lastname: Trusov
authors.forename: Daisuke
authors.initials: D
authors.lastname: Urano
authors.forename: Bastian
authors.initials: B
authors.lastname: Welter
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Ralph
authors.initials: R
authors.lastname: Panstruga
authors.forename: Joachim F
authors.initials: JF
authors.lastname: Uhrig
authors.forename: Alan M
authors.initials: AM
authors.lastname: Jones
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D030541:Databases, Genetic; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D005816:Genetic Complementation Test; D005838:Genotype; D054794:Glycomics; D047468:Immunoprecipitation; D009024:Morphogenesis / Q000235:genetics; D010641:Phenotype; D025941:Protein Interaction Mapping; D040901:Proteomics; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins