title: Genes differentially regulated by NKX2-3 in B cells between ulcerative colitis and Crohn's disease patients and possible involvement of EGR1.
abstract: Ulcerative colitis (UC) and Crohn's disease (CD) are two related yet different forms of chronic intestinal inflammation. We investigated the genes regulated by NKX2-3 in B cells from a UC patient by cDNA microarray and compared the results to those genes regulated by NKX2-3 in B cells from a CD patient. Genes regulated by NKX2-3 in B cells from UC were mainly involved in cell growth, inflammation, and immune response. Among the genes regulated by NKX2-3 in both UC and CD, expression of 145 genes was similarly altered and 34 genes was differentially affected by NKX2-3 knockdown. EGR1 was up-regulated in NKX2-3 knockdown B cells from UC while down-regulated in NKX2-3 knockdown B cells from CD. mRNA expressions of NKX2-3 and EGR1 were increased in diseased intestinal tissues from 19 CD patients. NKX2-3 may play different roles in UC and CD pathogenesis by differential regulation of EGR1.
authors.affiliation: Department of Colon and Rectal Surgery, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Zhenwu
authors.initials: Z
authors.lastname: Lin
authors.forename: John P
authors.initials: JP
authors.lastname: Hegarty
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Ashley A
authors.initials: AA
authors.lastname: Kelly
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Wang
authors.forename: Lisa S
authors.initials: LS
authors.lastname: Poritz
authors.forename: Walter A
authors.initials: WA
authors.lastname: Koltun
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D003093:Colitis, Ulcerative / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D003424:Crohn Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015536:Down-Regulation; D051766:Early Growth Response Protein 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D008875:Middle Aged; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D018398:Homeodomain Proteins; C534957:NKX2-3 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D014157:Transcription Factors