title: Antihyperglycemic activity and antidiabetic effect of methyl caffeate isolated from Solanum torvum Swartz. fruit in streptozotocin induced diabetic rats.
abstract: Natural remedies from medicinal plants are considered to be effective and safe alternatives to treat diabetes mellitus. Solanum torvum Swartz. fruit is widely used in the traditional system of medicine to treat diabetes. In the present study methyl caffeate, isolated from S. torvum fruit, was screened for its efficacy in controlling diabetes in animal models. Antihyperglycemic effect of methyl caffeate was studied in normal glucose-fed rats. The effects of oral administration of methyl caffeate (10, 20 and 40 mg/kg) for 28 days on body weight, fasting blood glucose, plasma insulin, hemoglobin, glycated hemoglobin, total protein, hepatic glycogen and carbohydrate metabolism enzymes in streptozotocin induced diabetic rats were investigated. Histological observations in the pancreas and GLUT4 expression in skeletal muscles were also studied. Methyl caffeate at 40 mg/kg significantly prevented the increase in blood glucose level after glucose administration at 60 min in comparison to the hyperglycemic control group. In streptozotocin induced diabetic rats, methyl caffeate produced significant reduction in blood glucose and increased body weight. The levels and/or activities of other biochemical parameters were near normal due to treatment with methyl caffeate. Methyl caffeate treated diabetic rats showed upregulation of GLUT4 and regeneration of β-cells in the pancreas. These results substantiated that methyl caffeate possessed hypoglycemic effect, and it could be developed into a potent oral antidiabetic drug.
authors.affiliation: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai 600 034, India.
authors.forename: Gopalsamy Rajiv
authors.initials: GR
authors.lastname: Gandhi
authors.forename: Savarimuthu
authors.initials: S
authors.lastname: Ignacimuthu
authors.forename: Michael Gabriel
authors.initials: MG
authors.lastname: Paulraj
authors.forename: Ponnusamy
authors.initials: P
authors.lastname: Sasikumar
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D005215:Fasting / Q000097:blood; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D005951:Glucose Tolerance Test; D051275:Glucose Transporter Type 4 / Q000235:genetics / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D032322:Solanum / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D001786:Blood Glucose; D002109:Caffeic Acids; D006597:Fructose-Bisphosphatase; D051275:Glucose Transporter Type 4; D005952:Glucose-6-Phosphatase; D006442:Glycated Hemoglobin; D006003:Glycogen; D006593:Hexokinase; D007004:Hypoglycemic Agents; D007328:Insulin; D012333:RNA, Messenger; C042405:methyl caffeate