title: Bilayer matrix composed of polycation/DNA complex and sodium alginate gel as a tumor cell catcher.
abstract: A bilayer matrix consisting of TABP-SS/DNA complexes and sodium alginate gel is formed via electrostatic interaction. In vitro cell adhesion, proliferation and transfection of the bilayer matrix are investigated in HepG2, HeLa and COS7 cells. Results show that this matrix can only promote tumor cell attachment and growth. Compared with normal cells, the bilayer matrix exhibits significantly higher transfection efficacy in tumor cells. Cell co-culture competitive transfection assay shows that the cell uptake of TABP-SS/DNA complexes is significantly enhanced in tumor cells rather than normal cells under the co-culture competitive condition, which confirms that TABP-SS/DNA complexes have strong tumor cell selectivity and tumor targeting transfection ability.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zeng
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D001360:Avidin / Q000737:chemistry / Q000378:metabolism; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D019556:COS Cells; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000494:pharmacology; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006367:HeLa Cells; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008156:Luciferases / Q000032:analysis; D058990:Molecular Targeted Therapy / Q000379:methods*; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011094:Polyethyleneimine / Q000737:chemistry; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism; D055672:Static Electricity; D014162:Transfection / Q000379:methods*
chemical_list: D000464:Alginates; D001360:Avidin; D001710:Biotin; D004247:DNA; D004337:Drug Carriers; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008156:Luciferases; D011073:Polyamines; D000071228:Polyelectrolytes; D011094:Polyethyleneimine; D011990:Receptors, Transferrin