title: Phytochemical and Bioactivity Studies on Constituents of the Leaves of Vitex Quinata.
abstract: A phytochemical investigation of the leaves of Vitex quinata (Lour.) F.N. Williams (Verbenaceae), guided by the MCF-7 human breast cancer cell line, led to the isolation of a new δ-truxinate derivative (1) and a new phytonoic acid derivative (2), together with 12 known compounds. The structures of the new compounds were determined by spectroscopic methods as dimethyl 3,4,3',4'-tetrahydroxy-δ-truxinate (1) and methyl 10R-methoxy-12-oxo-9(13),16E-phytodienoate (2), respectively. In a cytotoxicity assay, (S)-5-hydroxy-7,4'-dimethoxyflavanone (3) was found to be the sole active principle, with ED(50) values of 1.1-6.7 μM, respectively, when tested against a panel of three human cancer cells. Methyl 3,4,5-O-tricaffeoyl quinate (4) showed activity in an enzyme-based ELISA NF-κB p65 assay, with an ED(50) value of 10.3 μM.
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Ye
authors.initials: Y
authors.lastname: Deng
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
authors.forename: Hee-Byung
authors.initials: HB
authors.lastname: Chai
authors.forename: Esperanza Carcache
authors.initials: EC
authors.lastname: de Blanco
authors.forename: Leonardus B S
authors.initials: LB
authors.lastname: Kardono
authors.forename: Soedarsano
authors.initials: S
authors.lastname: Riswan
authors.forename: Djaja D
authors.initials: DD
authors.lastname: Soejarto
authors.forename: Norman R
authors.initials: NR
authors.lastname: Farnsworth
authors.forename: A Douglas
authors.initials: AD
authors.lastname: Kinghorn