title: Type I and III collagen degradation products in serum predict patient survival in head and neck squamous cell carcinoma.
abstract: Cancer invasion induces extracellular matrix remodeling and collagen degradation. The aim of this study was to assess whether serum levels of type I and III collagen degradation products were associated with patient survival in head and neck squamous cell carcinoma (HNSCC). A novel enzyme immunoassay was developed for measuring type III collagen N-terminal telopeptide (IIINTP) in human serum samples. In addition, type I collagen C-terminal telopeptide (ICTP), matrix metalloprotease-8 (MMP-8) and tissue inhibitor of metalloproteases-1 (TIMP-1) were assessed in 205 blood samples from HNSCC patients. High levels of serum ICTP and IIINTP and plasma TIMP-1 were associated with poor survival. The concentration of ICTP was associated with levels of IIINTP and TIMP-1. The plasma concentration of MMP-8 was associated with tumor stage, but not with survival or levels of ICTP, IIINTP or TIMP-1 suggesting that other collagenases/proteases are responsible for the cleavage of type I and type III collagens. The rate of type I and type III collagen degradation is associated with patient survival and can be used as a prognostic marker in HNSCC.
authors.affiliation: Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, Oulu University Central Hospital, FIN-90014 Oulu, Finland.
authors.forename: Sini
authors.initials: S
authors.lastname: Nurmenniemi
authors.forename: Marja-Kaisa
authors.initials: MK
authors.lastname: Koivula
authors.forename: Pia
authors.initials: P
authors.lastname: Nyberg
authors.forename: Taina
authors.initials: T
authors.lastname: Tervahartiala
authors.forename: Timo
authors.initials: T
authors.lastname: Sorsa
authors.forename: Petri S
authors.initials: PS
authors.lastname: Mattila
authors.forename: Tuula
authors.initials: T
authors.lastname: Salo
authors.forename: Juha
authors.initials: J
authors.lastname: Risteli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001681:Biological Assay / Q000379:methods; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000401:mortality; D024042:Collagen Type I / Q000097:blood* / Q000378:metabolism; D024061:Collagen Type III / Q000097:blood / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000401:mortality; D006801:Humans; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000097:blood; D008875:Middle Aged; D010455:Peptides / Q000097:blood*; D011379:Prognosis; D015996:Survival Rate; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000097:blood
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D010455:Peptides; D019715:Tissue Inhibitor of Metalloproteinase-1; C052929:collagen type I trimeric cross-linked peptide; D020784:Matrix Metalloproteinase 8