title: Differential expression of microRNA-2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm.
abstract: MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate a gene expression at the post-transcriptional level. Using in silico screening, we found that the 3'-untranslated regions of the P25 gene mRNA are perfectly complementary to nucleotides 2-8 at the 5' end of the miRNA-2b (miR-2b). The expression of miR-2b and the P25 gene in posterior silk gland of the fifth instar larval silkworm was investigated using real-time PCR detection method. The results indicated that expression of the P25 gene was very high in the posterior silk gland during the fifth instar larvae, whereas a level of miR-2b sharply decreased until reaching the lowest one on the 8th day. The expression patterns of miR-2b and P25 gene indicate that miR-2b might act as a fine-tuning regulator of expression of the P25 gene at the post-transcriptional level.
authors.affiliation: The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Jiang Su University of Science and Technology, 212018 Jiangsu Province, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Quan
authors.initials: Q
authors.lastname: Zou
authors.forename: Xing Jia
authors.initials: XJ
authors.lastname: Shen
authors.forename: Shun Ming
authors.initials: SM
authors.lastname: Tang
authors.forename: Sheng Peng
authors.initials: SP
authors.lastname: Wang
authors.forename: Qiao Ling
authors.initials: QL
authors.lastname: Zhao
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development; D019295:Computational Biology; D005349:Fibroins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000254:growth & development; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D034741:RNA, Small Interfering; C418613:fhx protein, Bombyx mori; D005349:Fibroins