title: Characterization of thermophilic halotolerant Aeribacillus pallidus TD1 from Tao Dam Hot Spring, Thailand.
abstract: The bacterial strain TD1 was isolated from Tao Dam hot spring in Thailand. Strain TD1 was Gram positive, rod-shaped, aerobic, motile, and endospore forming. The cell was 2.0-40 μm in length and about 0.4 μm in diameter. The optimum growth occurred at 55-60 °C and at pH 7-8. Strain TD1 was able to grow on medium containing up to 10% NaCl. The DNA G+C content was 38.9 mol%. The cellular fatty acid content was mainly C(16:0), which comprised 25.04% of the total amount of cellular fatty acid. 16S rDNA showed 99% identity to Aeribacillus pallidus DSM 3670(T). Bayesian tree analysis strongly supported the idea that strain TD1 is affiliated with genus Aeribacillus, as Aeribacillus pallidus strain TD1. Although the 16S rDNA of A. pallidus strain TD1 is similar to that of A. pallidus DSM 3670(T), some physiological properties and the cellular fatty acid profiles differ significantly. A. pallidus strain TD1 can produce extracellular pectate lyase, which has not been reported elsewhere for other bacterial strains in the genus Aeribacillus. A. pallidus strain TD1 may be a good candidate as a pectate lyase producer, which may have useful industrial applications.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand; E-Mails:  vimandin@hotmail.com  (M.Y.);  jeedkha@hotmail.com  (S.A.);  oom_arda@hotmail.com  (A.P.).
authors.forename: Montri
authors.initials: M
authors.lastname: Yasawong
authors.forename: Supatra
authors.initials: S
authors.lastname: Areekit
authors.forename: Arda
authors.initials: A
authors.lastname: Pakpitchareon
authors.forename: Somchai
authors.initials: S
authors.lastname: Santiwatanakul
authors.forename: Kosum
authors.initials: K
authors.lastname: Chansiri
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology* / Q000648:ultrastructure; D001482:Base Composition; D005227:Fatty Acids / Q000378:metabolism; D045482:Hot Springs / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013785:Thailand
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S