title: Synthesis and characterization of poly(N-hydroxyethylacrylamide) for long-term antifouling ability.
abstract: Development of biomaterials with long-term biocompatibility, durability, and stability remains a critical challenge for biomedical devices. Here, we synthesize, characterize, and graft poly(N-(2-hydroxyethyl)acrylamide) (polyHEAA) onto both gold surfaces and gold nanoparticles (AuNPs) via surface-initiated atom transfer radical polymerization (SI-ATRP) to form a stable antifouling coating to resist nonspecific protein adsorption and bacterial attachment. Surface plasmon resonance (SPR) results demonstrate that all of polyHEAA brushes coated on the gold substrate at a wide range of film thickness of ~10-40 nm can achieve almost zero protein adsorption from undiluted blood plasma and serum for 1 h, while static bacteria assay results show that polyHEAA brushes prohibit long-term bacterial colonization by Staphylococcus epidermidis and Escherichia coli RP437 up to 3 days. Moreover, the polyHEAA-coated AuNPs with different diameters remain their hydrodynamic sizes unchanged in human blood plasma and serum for up to 7 days. All these data indicate that polyHEAA can serve as promising biomaterials with long-term biocompatibility and durability suitable for applications in complex biological media.
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, Ohio 44325, United States.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D000327:Adsorption; D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry; D018441:Biofilms; D001798:Blood Proteins / Q000737:chemistry; D004926:Escherichia coli / Q000502:physiology; D006046:Gold / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013212:Staphylococcus epidermidis / Q000502:physiology; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D001798:Blood Proteins; D011108:Polymers; C074962:poly-N-(2-hydroxyethyl)acrylamide; D006046:Gold