title: Sonic hedgehog signaling promotes growth of oral squamous cell carcinoma cells associated with bone destruction.
abstract: Sonic hedgehog (Shh) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the role of Shh in bone destruction associated with oral squamous cell carcinomas, which frequently invade the maxilla or the mandible, is still unclear. In this study we show that the use of siRNA for Shh to block SHH secreted by SAS oral squamous cell carcinoma cells suppressed the tumor growth and tumor angiogenesis of subcutaneous SAS xenografts in vivo. Moreover, blockade of Shh in SAS cells decreased tumor growth and osteoclast number in a tibial metaphysis mouse model. Significantly, we clearly show that SHH stimulated osteoclast formation in a co-culture system consisting of murine bone stromal ST2 cells and murine CD11b(+) bone marrow cells. These findings suggest that Shh signaling is a potential target for the treatment of oral squamous cell carcinoma associated with bone destruction.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Kita-ku, Okayama 700-8525, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Honami
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Shimo
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Okui
authors.forename: Naito
authors.initials: N
authors.lastname: Kurio
authors.forename: Nur Mohammad Monsur
authors.initials: NM
authors.lastname: Hassan
authors.forename: Masahiro
authors.initials: M
authors.lastname: Iwamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D001862:Bone Resorption / Q000378:metabolism* / Q000473:pathology; D039481:CD11b Antigen; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009374:Neoplasms, Experimental; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D013977:Tibia / Q000473:pathology*
chemical_list: D039481:CD11b Antigen; D053823:Hedgehog Proteins; D034741:RNA, Small Interfering; C509239:SHH protein, human