title: Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins.
abstract: Budding yeast cell cycle oscillates between states of low and high cyclin-dependent kinase activity, driven by association of Cdk1 with B-type (Clb) cyclins. Various Cdk1-Clb complexes are activated and inactivated in a fixed, temporally regulated sequence, inducing the behaviour known as "waves of cyclins". The transition from low to high Clb activity is triggered by degradation of Sic1, the inhibitor of Cdk1-Clb complexes, at the entry to S phase. The G(1) phase is characterized by low Clb activity and high Sic1 levels. High Clb activity and Sic1 proteolysis are found from the beginning of the S phase until the end of mitosis. The mechanism regulating the appearance on schedule of Cdk1-Clb complexes is currently unknown. Here, we analyse oscillations of Clbs, focusing on the role of their inhibitor Sic1. We compare mathematical networks differing in interactions that Sic1 may establish with Cdk1-Clb complexes. Our analysis suggests that the wave-like cyclins pattern derives from the binding of Sic1 to all Clb pairs rather than from Clb degradation. These predictions are experimentally validated, showing that Sic1 indeed interacts and coexists in time with Clbs. Intriguingly, a sic1Δ strain looses cell cycle-regulated periodicity of Clbs, which is observed in the wild type, whether a SIC1-0P strain delays the formation of Clb waves. Our results highlight an additional role for Sic1 in regulating Cdk1-Clb complexes, coordinating their appearance.
authors.affiliation: Institute for Biology, Theoretical Biophysics, Humboldt University Berlin, Berlin, Germany. matteo.barberis@biologie.hu-berlin.de
authors.forename: Matteo
authors.initials: M
authors.lastname: Barberis
authors.forename: Christian
authors.initials: C
authors.lastname: Linke
authors.forename: Miquel À
authors.initials: MÀ
authors.lastname: Adrover
authors.forename: Alberto
authors.initials: A
authors.lastname: González-Novo
authors.forename: Hans
authors.initials: H
authors.lastname: Lehrach
authors.forename: Sylvia
authors.initials: S
authors.lastname: Krobitsch
authors.forename: Francesc
authors.initials: F
authors.lastname: Posas
authors.forename: Edda
authors.initials: E
authors.lastname: Klipp
mesh_terms: D001683:Biological Clocks; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002453:Cell Cycle / Q000502:physiology*; D003198:Computer Simulation; D019926:Cyclin B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000378:metabolism*; D025461:Feedback, Physiological; D008954:Models, Biological; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D019926:Cyclin B; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; C086926:SIC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D016203:CDC2 Protein Kinase