title: Binding of Sudan II and Sudan IV to bovine serum albumin: comparison studies.
abstract: In this paper, we report the interaction of Sudan II and Sudan IV to bovine serum albumin (BSA). Structural analysis showed that both Sudan II and Sudan IV interact mainly with BSA at the hydrophobic pocket and via Van der Waals forces. The number of bound Sudan molecule for each protein molecule was approximately 1. The overall binding constants at 293 K (20°C) estimated for Sudan II and Sudan IV were 1.22 × 10(4)M(-1) and 1.48 × 10(4)M(-1), respectively. BSA backbone structure was damaged by the dyes with more severe phenomenon observed for Sudan IV. For two Sudan dyes with the same concentration, Sudan IV could cause more alterations on CD spectra of BSA with slight decrease of α-helical content and increase of β-sheet content, suggesting a partial protein unfolding.
authors.affiliation: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Shandong Province, 27# Shanda South Road, Jinan 250100, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Lu
authors.forename: Xingchen
authors.initials: X
authors.lastname: Zhao
authors.forename: Yingcan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bingcong
authors.initials: B
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Mengyang
authors.initials: M
authors.lastname: Geng
authors.forename: Rutao
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D001391:Azo Compounds / Q000737:chemistry* / Q000633:toxicity*; D002417:Cattle; D002942:Circular Dichroism / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics
chemical_list: D001391:Azo Compounds; D012710:Serum Albumin, Bovine; C033003:sudan red; C009213:Scarlet Red