title: Mutation of OsALDH7 causes a yellow-colored endosperm associated with accumulation of oryzamutaic acid A in rice.
abstract: Aldehyde dehydrogenase proteins consist of a superfamily and the family 7 (ALDH7) is a typical group with highly conserved proteins across species. It catalyzes oxidation of α-aminoadipic semialdehyde (AASA) in lysine degradation, participates in protection against hyperosmotic stress, and detoxifies aldehydes in human; however, its function in plants has been much less documented. Here we reported a mutant with yellow-colored endosperm in rice, and showed that the yellow endosperm was caused by mutation of OsALDH7. OsALDH7 is expressed in all tissues detected, with the highest level in mature seeds. We found that oryzamutaic acid A accumulated during late seed development and after a year-long storage in the colored endosperm, whereas it was undetectable in the wild type endosperm. Moreover, lysine degradation was enhanced in yeast over-expressing OsALDH7 and as a result, content of lysine, glutamate and saccharopine was changed, suggesting a role of OsALDH7 in lysine catabolism.
authors.affiliation: State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. shenyi1202@gmail.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Lan
authors.forename: Linglong
authors.initials: L
authors.lastname: Liu
authors.forename: Shijia
authors.initials: S
authors.lastname: Liu
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Chen
authors.forename: Guixin
authors.initials: G
authors.lastname: Ren
authors.forename: Jianmin
authors.initials: J
authors.lastname: Wan
mesh_terms: D000143:Acids / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D000470:Alkaloids / Q000378:metabolism; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D003001:Cloning, Molecular; D056625:Endosperm / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D010858:Pigmentation / Q000235:genetics* / Q000502:physiology; D010860:Pigments, Biological / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D000143:Acids; D000470:Alkaloids; D010860:Pigments, Biological; C571934:oryzamutaic acid A; D018698:Glutamic Acid; D000444:Aldehyde Dehydrogenase; D008239:Lysine; C100169:saccharopine