title: Choice of resident costimulatory molecule can influence cell fate in human naïve CD4+ T cell differentiation.
abstract: With antigen stimulation, naïve CD4+ T cells differentiate to several effector or memory cell populations, and cytokines contribute to differentiation outcome. Several proteins on these cells receive costimulatory signals, but a systematic comparison of their differential effects on naïve T cell differentiation has not been conducted. Two costimulatory proteins, CD28 and ICAM-1, resident on human naïve CD4+ T cells were compared for participation in differentiation. Under controlled conditions, and with no added cytokines, costimulation through either CD3+CD28 or CD3+CAM-1 induced differentiation to T effector and T memory cells. In contrast, costimulation through CD3+ICAM-1 induced differentiation to Treg cells whereas costimulation through CD3+CD28 did not.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
authors.forename: Kelli M
authors.initials: KM
authors.lastname: Williams
authors.forename: Abby L
authors.initials: AL
authors.lastname: Dotson
authors.forename: Amber R
authors.initials: AR
authors.lastname: Otto
authors.forename: Jacob E
authors.initials: JE
authors.lastname: Kohlmeier
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Benedict
mesh_terms: D017951:Antigen Presentation; D018106:CD28 Antigens / Q000037:antagonists & inhibitors / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000378:metabolism; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000378:metabolism; D008213:Lymphocyte Activation; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508609:IL10 protein, human; C508594:IL2 protein, human; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10