title: Structural insights into the rhabdovirus transcription/replication complex.
abstract: The rhabdoviruses have a non-segmented single stranded negative-sense RNA genome. Their multiplication in a host cell requires three viral proteins in addition to the viral RNA genome. The nucleoprotein (N) tightly encapsidates the viral RNA, and the N-RNA complex serves as the template for both transcription and replication. The viral RNA-dependent RNA polymerase is a two subunit complex that consists of a large subunit, L, and a non-catalytic cofactor, the phosphoprotein, P. P also acts as a chaperone of nascent RNA-free N by forming a N(0)-P complex that prevents N from binding to cellular RNAs and from polymerizing in the absence of RNA. Here, we discuss the recent molecular and structural studies of individual components and multi-molecular complexes that are involved in the transcription/replication complex of these viruses with regard to their implication in viral transcription and replication.
authors.affiliation: UMI 3265 UJF-EMBL-CNRS Unit of Virus Host Cell Interactions, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.
authors.forename: Ivan
authors.initials: I
authors.lastname: Ivanov
authors.forename: Filip
authors.initials: F
authors.lastname: Yabukarski
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Marc
authors.initials: M
authors.lastname: Jamin
mesh_terms: D000818:Animals; D001665:Binding Sites; D005814:Genes, Viral; D006801:Humans; D008958:Models, Molecular; D009698:Nucleoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010944:Plants; D011485:Protein Binding; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012209:Rhabdoviridae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018353:Rhabdoviridae Infections / Q000821:virology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics
chemical_list: D009698:Nucleoproteins; D021122:Protein Subunits; D012367:RNA, Viral; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase