title: The effects of iloprost on colonic anastomotic healing in rats.
abstract: OBJECTIVE
  The purpose of this experimental study is to investigate the effects of iloprost on colonic anastomotic healing in rats, after intraperitoneal administration.
  
  
  METHODS
  Forty male Albino-Wistar rats were randomized into two groups of twenty animals each. They all underwent colonic resection followed by an inverted anastomosis. The rats of Group A (control) received 3 ml of NaCl intraperitoneally, while those of Group B (iloprost) received iloprost (2 μg/kg body weight), immediately postoperatively and daily until killed. Each group was further divided into two equal subgroups, depending on the day of killing. The animals of subgroups 1 were killed on the fourth postoperative day, while those of subgroups 2 on the eighth. Macroscopical and histological assessments were performed. Besides, anastomotic bursting pressures and the tissue concentrations in hydroxyproline and collagenase I were also evaluated.
  
  
  RESULTS
  No anastomotic dehiscence was noted. The mean bursting pressure was higher in the iloprost group compared with the control group, but a significant difference was revealed only on the fourth postoperative day. Furthermore, iloprost significantly increased the new vessel formation on the fourth, as well as on the eighth postoperative day.
  
  
  CONCLUSIONS
  Iloprost enhances the early phase of colonic anastomotic healing in rats.
authors.affiliation: 4th Surgical Department, Aristotle University of Thessaloniki, 2 Macedonias Str, PO 55535, Pilea, Thessaloniki, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Galanopoulos
authors.forename: M G
authors.initials: MG
authors.lastname: Pramateftakis
authors.forename: D
authors.initials: D
authors.lastname: Raptis
authors.forename: I
authors.initials: I
authors.lastname: Mantzoros
authors.forename: D
authors.initials: D
authors.lastname: Kanellos
authors.forename: S
authors.initials: S
authors.lastname: Angelopoulos
authors.forename: G
authors.initials: G
authors.lastname: Koliakos
authors.forename: T
authors.initials: T
authors.lastname: Zaraboukas
authors.forename: C
authors.initials: C
authors.lastname: Lazaridis
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D017364:Collagenases / Q000032:analysis / Q000187:drug effects; D003106:Colon / Q000098:blood supply / Q000737:chemistry / Q000473:pathology / Q000601:surgery*; D006909:Hydroxyproline / Q000032:analysis / Q000187:drug effects; D016285:Iloprost / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011312:Pressure / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012421:Rupture / Q000209:etiology; D013997:Time Factors; D000267:Tissue Adhesions / Q000209:etiology; D014665:Vasodilator Agents / Q000009:adverse effects / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014665:Vasodilator Agents; D017364:Collagenases; C417249:collagenase 1; D016285:Iloprost; D006909:Hydroxyproline