title: Structure of the vesicular stomatitis virus N⁰-P complex.
abstract: Replication of non-segmented negative-strand RNA viruses requires the continuous supply of the nucleoprotein (N) in the form of a complex with the phosphoprotein (P). Here, we present the structural characterization of a soluble, heterodimeric complex between a variant of vesicular stomatitis virus N lacking its 21 N-terminal residues (N(Δ21)) and a peptide of 60 amino acids (P(60)) encompassing the molecular recognition element (MoRE) of P that binds RNA-free N (N(0)). The complex crystallized in a decameric circular form, which was solved at 3.0 Å resolution, reveals how the MoRE folds upon binding to N and competes with RNA binding and N polymerization. Small-angle X-ray scattering experiment and NMR spectroscopy on the soluble complex confirms the binding of the MoRE and indicates that its flanking regions remain flexible in the complex. The structure of this complex also suggests a mechanism for the initiation of viral RNA synthesis.
authors.affiliation: UMI 3265 UJF-EMBL-CNRS Unit of Virus Host Cell Interactions, Grenoble, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Leyrat
authors.forename: Filip
authors.initials: F
authors.lastname: Yabukarski
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tarbouriech
authors.forename: Euripedes A
authors.initials: EA
authors.lastname: Ribeiro
authors.forename: Malene Ringkjøbing
authors.initials: MR
authors.lastname: Jensen
authors.forename: Martin
authors.initials: M
authors.lastname: Blackledge
authors.forename: Rob W H
authors.initials: RW
authors.lastname: Ruigrok
authors.forename: Marc
authors.initials: M
authors.lastname: Jamin
mesh_terms: D018360:Crystallography, X-Ray; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D019590:Nucleocapsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D012367:RNA, Viral / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D038621:Regulatory Sequences, Ribonucleic Acid / Q000502:physiology; D018116:Vesiculovirus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D046912:Multiprotein Complexes; D019590:Nucleocapsid Proteins; C081250:P protein, Vesicular stomatitis virus; D010750:Phosphoproteins; D012367:RNA, Viral; D038621:Regulatory Sequences, Ribonucleic Acid; D015678:Viral Structural Proteins