title: Isolation of DNA aptamers using micro free flow electrophoresis.
abstract: A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55414, USA.
authors.forename: Meng
authors.initials: M
authors.lastname: Jing
authors.forename: Michael T
authors.initials: MT
authors.lastname: Bowser
mesh_terms: D052157:Aptamers, Nucleotide / Q000302:isolation & purification* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D019075:Electrophoresis, Capillary; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D011485:Protein Binding; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D007073:Immunoglobulin E