title: Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells.
abstract: Retinoic acid (RA) is able to induce the differentiation of embryonic stem cells into neuronal lineages. The mechanism of this effect is unknown but it has been evidenced to be dependent on the formation of floating spheroids called embryoid bodies. Results presented here show that the inhibition of phosphoinositide 3-kinase signaling pre-determines mouse embryonic stem cells to RA induced neurogenesis in monolayer culture with no need of embryoid bodies formation.
authors.affiliation: Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Kotasová
authors.forename: Iva
authors.initials: I
authors.lastname: Veselá
authors.forename: Jan
authors.initials: J
authors.lastname: Kučera
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Houdek
authors.forename: Jiřina
authors.initials: J
authors.lastname: Procházková
authors.forename: Milena
authors.initials: M
authors.lastname: Králičková
authors.forename: Jiří
authors.initials: J
authors.lastname: Pacherník
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017930:Genes, Reporter; D053533:Keratin-8 / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009025:Morpholines / Q000494:pharmacology*; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D000943:Antigens, Differentiation; D015820:Cadherins; D002867:Chromones; D053533:Keratin-8; D009025:Morpholines; D019006:Neural Cell Adhesion Molecules; D000081082:Phosphoinositide-3 Kinase Inhibitors; D014404:Tubulin; C462941:beta3 tubulin, mouse; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D014212:Tretinoin; D008156:Luciferases; D058539:Phosphatidylinositol 3-Kinase