title: Uterobrush method in the detection of endometrial pathology.
abstract: BACKGROUND
  Endometrial brush cytology is a widely accepted method for the detection of endometrial lesions. The aim of this study was to evaluate the role of cytological sampling using Uterobrush in the screening of endometrial pathology.
  
  
  METHODS
  This is a prospective double-blind study evaluating the efficacy of the Uterobrush method (Cooper Surgical, Trumbull, USA) in the detection of endometrial abnormalities. Endometrial cytology was performed during the period January 2009 to April 2010 in all symptomatic patients that underwent dilatation and curettage. The collected samples were firstly smeared directly onto a glassslide and consequently into Thin-Prep buffer. Cytologic features were evaluated according to the criteria of Tao. The main objective was to evaluate the efficacy of Uterobrush method comparing the results of cytologic and histopathologic examination.
  
  
  RESULTS
  The sample of the study consisted of 100 women aged 55.8 years (range 38-78 years) with recorded data regarding Uterobrush test and classic histologic examination. Fifty-five patients were postmenopausal. A total of 92% of the samplings were performed by trainees. Endometrial carcinoma was cytologically diagnosed in 8/9 patients, whereas endometrial polyps were diagnosed in 5/34 patients (14.7%). All the patients with simple hyperplasia were correctly diagnosed with the Uterobrush method, whereas the diagnosis of complex hyperplasia with or without atypia was correct in 85.7% and 100% of patients, respectively. Regarding endometrial carcinoma, the sensitivity, specificity, positive and negative predictive values were 88.9%, 100%, 100% and 98.9%, respectively. On the other hand, regarding endometrial polyps, the sensitivity, specificity, positive and negative predictive values were 14.7%, 100%, 100% and 69.5%, respectively.
  
  
  CONCLUSIONS
  Uterobrush is a reliable direct intrauterine sampling for detecting endometrial abnormalities especially endometrial carcinoma and hyperplasia, but not endometrial polyps. It is a well-tolerated, easy to use method, which provides generous endometrial sampling without contamination from the endocervix or the vagina.
authors.affiliation: Second Department of Obstetrics and Gynecology, University of Athens, Aretaieio Hospital, Athens, Greece. christosiavazzo@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Iavazzo
authors.forename: G
authors.initials: G
authors.lastname: Vorgias
authors.forename: G
authors.initials: G
authors.lastname: Mastorakos
authors.forename: G
authors.initials: G
authors.lastname: Stefanatou
authors.forename: A
authors.initials: A
authors.lastname: Panoussi
authors.forename: A
authors.initials: A
authors.lastname: Alexiadou
authors.forename: S
authors.initials: S
authors.lastname: Plyta
authors.forename: C
authors.initials: C
authors.lastname: Lekka
authors.forename: N
authors.initials: N
authors.lastname: Kalinoglou
authors.forename: V
authors.initials: V
authors.lastname: Dertimas
authors.forename: T
authors.initials: T
authors.lastname: Akrivos
authors.forename: S
authors.initials: S
authors.lastname: Fotiou
mesh_terms: D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000473:pathology; D003581:Cytodiagnosis / Q000379:methods*; D003710:Demography; D016889:Endometrial Neoplasms / Q000175:diagnosis / Q000473:pathology; D004717:Endometrium / Q000473:pathology*; D005260:Female; D006801:Humans; D008875:Middle Aged