title: A novel glycosaminoglycan-like polysaccharide from abalone Haliotis discus hannai Ino: purification, structure identification and anticoagulant activity.
abstract: A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (M(w)) was 56.2 kDa in a TSK G4000 column. Further IR, 1D, 2D NMR spectroscopic analysis of the correlation signals of different sugar units gave a proposal repeating structure, as follows: [chemical structure: see the text] In vitro anticoagulant assay indicated AAP prolonged both the activated partial thromboplastin time (APTT) and thrombin time (TT), with a 22.5 U/mg and 72.0 U/mg compared with standard heparin, respectively. The anticoagulant property of AAP was mainly attributed to powerful potentiation thrombin by anti-thrombin III.
authors.affiliation: College of Food Science and Technology, Ocean University of China, 5 Yu Shan Road, Qingdao, Shandong 266003, China.
authors.forename: Guoyun
authors.initials: G
authors.lastname: Li
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Xue
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Chang
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Li
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D002240:Carbohydrate Sequence; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D015951:Factor Xa / Q000378:metabolism; D005643:Fucose / Q000737:chemistry; D049851:Gastropoda / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013431:Sulfates / Q000737:chemistry; D013917:Thrombin / Q000378:metabolism; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates; D005643:Fucose; D020723:Glucuronic Acid; D006493:Heparin; D013917:Thrombin; D015951:Factor Xa