title: Transcriptome analysis of an oil-rich race B strain of Botryococcus braunii (BOT-22) by de novo assembly of pyrosequencing cDNA reads.
abstract: To gain genetic insights into the biosynthesis of botryococcene oils in Botryococcus braunii race B, a transcriptome dataset of the BOT-22 strain containing 27,427 non-redundant sequences assembled from 209,429 complementary DNA reads was obtained via high-throughput 454 sequencing. Relatively reliable prediction of the gene product was feasible for 725 non-redundant sequences based on homology to previously characterized database sequences. Regarding the botryococcene oil biosynthesis, genes putatively associated with the mevalonate-independent isoprenoid biosynthesis pathway were retrieved, while no genes were found for the mevalonate pathway, suggesting that botryococcenes are biosynthesized through the mevalonate-independent pathway in B. braunii. All transcriptome sequences have been deposited in the GenBank/EMBL/DDBJ database.
authors.affiliation: Environmental Biology Division, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-8506, Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Ioki
authors.forename: Masato
authors.initials: M
authors.lastname: Baba
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Nakajima
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shiraiwa
authors.forename: Makoto M
authors.initials: MM
authors.lastname: Watanabe
mesh_terms: D001483:Base Sequence; D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013696:Temperature
chemical_list: D018076:DNA, Complementary; D010938:Plant Oils