title: Allelic polymorphism in the second exon of Ovar-DRB1 in fat-tailed sheep.
abstract: Ovar-DRB1 is one of the most important response genes in the major histocompatibility complex (MHC) class II region of sheep. Gene polymorphism in the second exon of Ovar-DRB1 in three different Iranian fat-tailed sheep breeds (Lori-Bakhtiari, Shaul and Zandi) was analyzed by either restriction fragment length polymorphisms (RFLP) or direct sequencing. A total of 92 Lori-Bakhtiari, 40 Shaul, and 47 Zandi sheep were examined. PCR-RFLP identified 17 genotypes in Lori-Bakhtiari sheep, 12 in Shaul sheep and 11 in Zandi sheep. Collectively, 24 different genotypes could be found for Iranian fat-tailed sheep. Using direct sequencing, seven new sequences in exon 2 of the Ovar-DRB1 gene were identified. Generalized linear modeling with a multinomial error structure showed that the sheep populations had significantly different allele frequencies.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. nikbakht@ut.ac.ir
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Nikbakht
authors.forename: Hossein
authors.initials: H
authors.lastname: Rezaii
authors.forename: Michel J
authors.initials: MJ
authors.lastname: Stear
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Talebi
authors.forename: Homayoun
authors.initials: H
authors.lastname: Mahmoudzadeh
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics; D005802:Genes, MHC Class II / Q000235:genetics*; D005838:Genotype; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment / Q000662:veterinary; D012756:Sheep / Q000145:classification / Q000235:genetics*