title: One step immobilization of peptides and proteins by using modified parylene with formyl groups.
abstract: One-step immobilization method for peptides and proteins is developed by using modified parylene film with formyl groups which is suitable for microplate-based immunoassay and SPR biosensor application. The immobilization of peptides and proteins is achieved through the covalent bonding of the formyl group with the primary amine groups of peptides and proteins, which no additional activation step is required. In this work, the immobilization efficiency of parylene-H is estimated in comparison with parylene-A and physical adsorption, using biotinylated-cyclic citrullinated peptide (biotinylated-CCP), human chorionic gonadotropin (hCG) and horseradish peroxidase (HRP) as model proteins. The applicability of parylene-H film to SPR biosensor is demonstrated by estimating the detection range and sensitivity of SPR biosensor at various thicknesses. The immobilization efficiency of parylene-H film for SPR biosensor was compared with physical adsorption by using HRP as a model protein.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Ko
authors.forename: Eun-Hang
authors.initials: EH
authors.lastname: Lee
authors.forename: Ga-Yeon
authors.initials: GY
authors.lastname: Lee
authors.forename: Joil
authors.initials: J
authors.lastname: Kim
authors.forename: Byung-Jin
authors.initials: BJ
authors.lastname: Jeon
authors.forename: Moo-Hwan
authors.initials: MH
authors.lastname: Kim
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D014992:Xylenes / Q000737:chemistry*
chemical_list: D011108:Polymers; D014992:Xylenes; C011055:parylene; D006735:Horseradish Peroxidase