title: Composition analysis and material characterization of an emulsifying extracellular polysaccharide (EPS) produced by Bacillus megaterium RB-05: a hydrodynamic sediment-attached isolate of freshwater origin.
abstract: OBJECTIVE
  This work was aimed to isolate, purify and characterize an extracellular polysaccharide (EPS) produced by a freshwater dynamic sediment-attached micro-organism, Bacillus megaterium RB-05, and study its emulsifying potential in different hydrocarbon media.
  
  
  RESULTS
  Bacillus megaterium RB-05 was found to produce EPSs in glucose mineral salts medium, and maximum yield (0.864 g l(-1) ) was achieved after 24-h incubation. The recovery rates of the polysaccharide material by ion-exchange and gel filtration chromatography were around 67 and 93%, respectively. As evident from HPLC and FT-IR analyses, the polysaccharide was found to be a heteropolymer-containing glucose, galactose, mannose, arabinose, fucose and N-acetyl glucosamine. Different oligosaccharide combinations namely hexose(3), hexose(4), hexose(5) deoxyhexose(1) and hexose(5) deoxyhexose(1) pentose(3) were obtained after partial hydrolysis of the polymer using MALDI-ToF-MS. The polysaccharide with an average molecular weight of 170 kDa and thermal stability up to 180°C showed pseudoplastic rheology and significant emulsifying activity in hydrocarbon media.
  
  
  CONCLUSIONS
  Isolated polysaccharide was found to be of high molecular weight and thermally stable. The purified EPS fraction was composed of hexose, pentose and deoxyhexose sugar residues, which is a rare combination for bacterial polysaccharides. Emulsifying property was either better or comparable to that of other commercially available natural gums and polysaccharides.
  
  
  CONCLUSIONS
  This is probably one of the few reports about characterizing an emulsifying EPS produced by a freshwater sediment-attached bacterium. The results of this study contribute to understand the influence of chemical composition and material properties of a new microbial polysaccharide on its application in industrial biotechnology. Furthermore, this work reconfirms freshwater dynamic sediment as a potential habitat for bioprospecting extracellular polymer-producing bacteria. This study will improve our knowledge on the exploitation of a nonconventional renewable resource, which also seems to be ecologically significant.
authors.affiliation: Materials Science Centre, Indian Institute of Technology Kharagpur, Kharagpur, WB, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Chowdhury
authors.forename: S
authors.initials: S
authors.lastname: Manna
authors.forename: P
authors.initials: P
authors.lastname: Saha
authors.forename: R K
authors.initials: RK
authors.lastname: Basak
authors.forename: R
authors.initials: R
authors.lastname: Sen
authors.forename: D
authors.initials: D
authors.lastname: Roy
authors.forename: B
authors.initials: B
authors.lastname: Adhikari
mesh_terms: D001410:Bacillus megaterium / Q000737:chemistry* / Q000302:isolation & purification; D003470:Culture Media / Q000737:chemistry; D045703:Emulsifying Agents / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D005110:Extracellular Space / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology; D005947:Glucose / Q000032:analysis; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D012212:Rheology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D045703:Emulsifying Agents; D011135:Polysaccharides, Bacterial; D005947:Glucose