title: [Different action of the APETALA1 gene on the development of reproductive organs in flowers of the abruptus mutant of Arabidopsis thaliana (L.) Heynh].
abstract: The APETALA1 (AP1) gene of A. thaliana codes type II MADS protein with domains MADS, I, K, and C. The role of K- and C-domains in the functioning of AP1 protein is poorly investigated. The analysis of phenotypic manifestation of mutations disrupting the activity of various domains of the protein product allows us to obtain information on the function of domains and, thereby, on the structural-functional organization of the gene. We investigated the action of mutant alleles of the AP1 gene whose protein products are probably lacking the functionally active domains K (ap1-20), K- and C-domains (ap1-1 and ap1-6), and C-domain (ap 1-3) on the flower morphology in abr mutant (the ABRUPTUS/PINOID gene allele). It was detected that, unlike the ap 1-20 allele, the presence of ap 1-3, ap1-6, and ap 1-1 alleles results in reduction of a number of the generative organs in the flowers of the double mutants abr ap1-3, abr ap1-6, and abr ap1-1. It was suggested that C-domain of the AP1 protein prevents the alteration of determination of the type of reproductive organs at ectopic expression of the AP1 gene in the inner whorls of a flower in the abr mutant.
authors.forename: U N
authors.initials: UN
authors.lastname: Kavaĭ-ool
authors.forename: E V
authors.initials: EV
authors.lastname: Kupriianova
authors.forename: T A
authors.initials: TA
authors.lastname: Ezhova
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000166:cytology / Q000196:embryology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis* / Q000235:genetics; D035264:Flowers / Q000196:embryology* / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D026161:MADS Domain Proteins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D017434:Protein Structure, Tertiary
chemical_list: C091535:AP1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins