title: Immunohistochemical study of arginase 1 and 2 in various tissues of rats.
abstract: Arginase 1 and arginase 2 catalyze the hydrolysis of arginine to ornithine and urea. The localization of these enzymes was studied in various tissues in Sprague-Dawley rats by immunohistochemistry and Western blotting. Western blot analysis showed that both arginase 1 and 2 were differentially expressed in the various organs examined. Arginase 1 was expressed at high levels in the liver, at moderate levels in the pancreas, and at low levels in the cerebrum, cerebellum, spinal cord, stomach, small and large intestines, kidneys, lungs, and spleen. The levels of arginase 2 immunoreactivity were high in the kidneys and pancreas, and moderate in the cerebrum, spinal cord, stomach, small intestine, large intestine, and lungs; the levels were very low in the liver and spleen compared with that in the cerebellum. Immunohistochemical analysis largely confirmed the results of the Western blot analysis. These findings indicate that the levels of arginase 1 and 2 varied among organs, suggesting that the arginase isoforms may play organ-specific roles in the urea cycle.
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Republic of Korea.
authors.forename: Sungyoung
authors.initials: S
authors.lastname: Choi
authors.forename: Changnam
authors.initials: C
authors.lastname: Park
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.forename: Jun Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D001119:Arginase / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D002531:Cerebellum / Q000737:chemistry; D054022:Cerebrum / Q000737:chemistry; D007150:Immunohistochemistry; D007420:Intestine, Large / Q000737:chemistry; D007421:Intestine, Small / Q000737:chemistry; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D010179:Pancreas / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry; D013154:Spleen / Q000737:chemistry; D013270:Stomach / Q000737:chemistry
chemical_list: C113162:Arg2 protein, rat; D001119:Arginase