title: Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli.
abstract: Two genes, MGFc                         (40) and MGFc                         (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatography using His60 Ni Superflow. Recombinant MGFc(24) protein was purified using a glutathione-Sepharose 4B column. After enzymatic cleavage of the GST-tail, 1 mg MGFc(24) protein l(-1) was obtained. MGFc(40) and MGFc(24), which are involved in proliferation and differentiation, stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.
authors.affiliation: Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, China. lsong@sxu.edu.cn
authors.forename: Li
authors.initials: L
authors.lastname: Song
authors.forename: Wen-Si
authors.initials: WS
authors.lastname: Zhu
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Dong
authors.forename: Zhuo-Yu
authors.initials: ZY
authors.lastname: Li
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; C523997:mechano-growth factor E, human; D007334:Insulin-Like Growth Factor I