title: Transposon Ac/Ds-induced chromosomal rearrangements at the rice OsRLG5 locus.
abstract: Previous studies have shown that pairs of closely-linked Ac/Ds transposable elements can induce various chromosomal rearrangements in plant genomes. To study chromosomal rearrangements in rice, we isolated a line (OsRLG5-161) that contains two inversely-oriented Ds insertions in OsRLG5 (Oryza sativa Receptor like kinase Gene 5). Among approximately 300 plants regenerated from OsRLG5-161 heterozygous seeds, 107 contained rearrangements including deletions, duplications and inversions of various sizes. Most rearrangements were induced by previously identified alternative transposition mechanism. Furthermore, we also detected a new class of rearrangements that contain juxtaposed inversions and deletions on the same chromosome. We propose that these novel alleles were generated by a previously unreported type of alternative transposition reactions involving the 5' and 3' termini of two inversely-oriented Ds elements located on the same chromatid. Finally, 11% of rearrangements contained inversions resulting from homologous recombination between the two inverted Ds elements in OsRLG5-161. The high frequency inheritance and great variety of rearrangements obtained suggests that the rice regeneration system results in a burst of transposition activity and a relaxation of the controls which normally limit the transposition competence of individual Ds termini. Together, these results demonstrate a greatly enlarged potential of the Ac/Ds system for plant chromosome engineering.
authors.affiliation: Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 660-701, Korea.
authors.forename: Yuan Hu
authors.initials: YH
authors.lastname: Xuan
authors.forename: Hai Long
authors.initials: HL
authors.lastname: Piao
authors.forename: Byoung Il
authors.initials: BI
authors.lastname: Je
authors.forename: Soon Ju
authors.initials: SJ
authors.lastname: Park
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Zhang
authors.forename: Thomas
authors.initials: T
authors.lastname: Peterson
authors.forename: Chang-deok
authors.initials: CD
authors.lastname: Han
mesh_terms: D002842:Chromatids / Q000235:genetics; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D032461:Chromosomes, Plant; D004251:DNA Transposable Elements; D017343:Genes, Plant; D056426:Genetic Loci; D059765:Homologous Recombination; D012275:Oryza / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements