title: Multi-host ectomycorrhizal fungi are predominant in a Guinean tropical rainforest and shared between canopy trees and seedlings.
abstract: The diversity of ectomycorrhizal (ECM) fungi on adult trees and seedlings of five species, Anthonotha fragrans, Anthonotha macrophylla, Cryptosepalum tetraphyllum, Paramacrolobium coeruleum and Uapaca esculenta, was determined in a tropical rain forest of Guinea. Ectomycorrhizae were sampled within a surface area of 1600 m(2), and fungal taxa were identified by sequencing the rDNA Internal Transcribed Spacer region. Thirty-nine ECM fungal taxa were determined, of which 19 multi-hosts, 9 single-hosts and 11 singletons. The multi-host fungi represented 92% (89% when including the singletons in the analysis) of the total abundance. Except for A. fragrans, the adults of the host species displayed significant differentiation for their fungal communities, but their seedlings harboured a similar fungal community. These findings suggest that there was a potential for the formation of common mycorrhizal networks in close vicinity. However, no significant difference was detected for the δ(13)C and δ(15)N values between seedlings and adults of each ECM plant, and no ECM species exhibited signatures of mixotrophy. Our results revealed (i) variation in ECM fungal diversity according to the seedling versus adult development stage of trees and (ii) low host specificity of ECM fungi, and indicated that multi-host fungi are more abundant than single-host fungi in this forest stand.
authors.affiliation: Laboratoire des Symbioses Tropicales et Méditerranéennes, UMR113 - INRA/AGRO-M/CIRAD/IRD/UM2 - TA10/J, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. fungalmolecol@gmail.com
authors.forename: Abdala Gamby
authors.initials: AG
authors.lastname: Diédhiou
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Selosse
authors.forename: Antoine
authors.initials: A
authors.lastname: Galiana
authors.forename: Moussa
authors.initials: M
authors.lastname: Diabaté
authors.forename: Bernard
authors.initials: B
authors.lastname: Dreyfus
authors.forename: Amadou Moustapha
authors.initials: AM
authors.lastname: Bâ
authors.forename: Sergio Miana
authors.initials: SM
authors.lastname: De Faria
authors.forename: Gilles
authors.initials: G
authors.lastname: Béna
mesh_terms: D044822:Biodiversity; D002247:Carbon Isotopes / Q000032:analysis; D004271:DNA, Fungal / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*; D016701:Guinea; D038821:Mycorrhizae / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D009587:Nitrogen Isotopes / Q000032:analysis; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*; D013045:Species Specificity; D014197:Trees / Q000235:genetics / Q000254:growth & development / Q000382:microbiology*
chemical_list: D002247:Carbon Isotopes; D004271:DNA, Fungal; D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D009587:Nitrogen Isotopes