title: Overexpression of minichromosome maintenance 2 predicts poor prognosis             in patients with gastric cancer.
abstract: We examined the expression of minichromosome maintenance 2 (MCM2) in gastric             cancer and adjacent normal tissues and estimated the possible value of MCM2 as             a novel prognostic marker. Using real-time PCR, western blotting and immunohistochemistry,             we examined the expression of MCM2 in gastric carcinoma and paired normal gastric             mucosa. Statistical analysis of the expression of MCM2 mRNA and protein in gastric             cancer and normal tissues was performed to evaluate the relationship between MCM2             expression and clinicopathological characteristics in gastric cancer. The expression             of MCM2 mRNA and protein in gastric carcinomas was significantly higher compared             to that in normal gastric mucosa (P=0.04). Immunohistochemistry analysis showed             that MCM2 expression was significantly up-regulated in tumor and metastastic lymph             node tissues compared with the corresponding non-cancerous mucosa (P<0.05).             Positive expression of MCM2 was significantly associated with patient age, T category             and the presence of lymph node metastasis (P<0.05). There were no differences             between MCM2 expression and gender, tumor size, tumor location, M category, International             Union Against Cancer (UICC) stage, vessel invasion and tumor differentiation.             Patients with negative tumor MCM2 expression displayed a better survival time             than those with positive MCM2 expression (P<0.05). Survival analysis showed             that positive MCM2 expression (P<0.05), T stage (P<0.05) and N stage (P<0.05)             were independent prognostic factors for disease-free survival (DFS) and overall             survival (OS). Our data suggest that MCM2 could serve as a novel prognostic biomarker             in gastric carcinoma.
authors.affiliation: Department of Anal-Colorectal Surgery, Affiliated Hospital of Ningxia Medical University, Yinchuan 750004, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Yugang
authors.initials: Y
authors.lastname: Wen
authors.forename: Hai
authors.initials: H
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
authors.forename: Ningmei
authors.initials: N
authors.lastname: Zhang
authors.forename: Xinrong
authors.initials: X
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Huamei
authors.initials: H
authors.lastname: Tang
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Peng
authors.forename: Yinxue
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2