title: Sauchinone suppresses pro-inflammatory mediators by inducing heme oxygenase-1 in RAW264.7 macrophages.
abstract: Sauchinone, a biologically active lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on bone resorption. In this study, we investigated the effect of sauchinone in suppressing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to a reduction in COX-2-derived prostaglandin E(2) (PGE(2)) and iNOS-derived nitric oxide (NO) production in lipopolysaccharide (LPS) stimulated RAW264.7 macrophages. Present study also demonstrates the effects of sauchinone in inducing heme oxygenase-1 (HO-1) expression and an increase in heme oxygenase (HO) activity in RAW264.7 macrophages. The effects of sauchinone on LPS-induced PGE(2), NO, tumor necrosis factor-α (TNF-α) and interlukine-1β (IL-1β) production were partially reversed by the HO-1 inhibitor Tin protoporphyrin was also seen in this study. In addition, we found that treatment with extracellular signal-regulated kinase (ERK) inhibitor (PD98059) reduced sauchinone-induced HO-1 expression. Sauchinone also increased ERK phosphorylation. These results suggest that sauchinone inhibits pro-inflammatory mediators through expression of anti-inflammatory HO-1 via ERK pathway.
authors.affiliation: Standardized Material Bank for New Botanical Drugs, College of Pharmacy, Wonkwang University, Iksan 570–749, Republic of Korea.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Hyun-Gyu
authors.initials: HG
authors.lastname: Choi
authors.forename: Kyoung-Su
authors.initials: KS
authors.lastname: Kim
authors.forename: Dae-Gil
authors.initials: DG
authors.lastname: Kang
authors.forename: Ho-Sub
authors.initials: HS
authors.lastname: Lee
authors.forename: Gil-Saeng
authors.initials: GS
authors.lastname: Jeong
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D001578:Benzopyrans / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000276:immunology / Q000378:metabolism; D004149:Dioxoles / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000378:metabolism / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology; D051547:Heme Oxygenase-1 / Q000276:immunology / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000503:physiopathology; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008665:Metalloporphyrins; D051379:Mice; D058990:Molecular Targeted Therapy; D009569:Nitric Oxide / Q000096:biosynthesis / Q000276:immunology; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D008517:Phytotherapy; D028321:Plant Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D011524:Protoporphyrins; D032134:Saururaceae; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D004149:Dioxoles; D004791:Enzyme Inhibitors; D005419:Flavonoids; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D008665:Metalloporphyrins; D028321:Plant Preparations; D011524:Protoporphyrins; D014409:Tumor Necrosis Factor-alpha; C410958:sauchinone; D009569:Nitric Oxide; C032628:tin protoporphyrin IX; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; D051547:Heme Oxygenase-1; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one