title: Linking specific heterotrophic bacterial populations to bioreduction of uranium and nitrate in contaminated subsurface sediments by using stable isotope probing.
abstract: Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [¹³C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.
authors.affiliation: Florida State University, Tallahassee, Florida 32306, USA.
authors.forename: Denise M
authors.initials: DM
authors.lastname: Akob
authors.forename: Lee
authors.initials: L
authors.lastname: Kerkhof
authors.forename: Kirsten
authors.initials: K
authors.lastname: Küsel
authors.forename: David B
authors.initials: DB
authors.lastname: Watson
authors.forename: Anthony V
authors.initials: AV
authors.lastname: Palumbo
authors.forename: Joel E
authors.initials: JE
authors.lastname: Kostka
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001711:Biotransformation; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D004785:Environmental Pollutants / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D052836:Heterotrophic Processes; D007553:Isotope Labeling / Q000379:methods; D009566:Nitrates / Q000378:metabolism*; D010084:Oxidation-Reduction; D012150:Polymorphism, Restriction Fragment Length; D014501:Uranium / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004785:Environmental Pollutants; D005290:Ferric Compounds; D009566:Nitrates; D000431:Ethanol; D014501:Uranium