title: Acute phase response and oxidative stress status in familial Mediterranean fever (FMF).
abstract: We aimed to determine acute phase response (APR) and oxidative stress in patients with familial Mediterranean fever (FMF) and compare these characteristics with those in healthy controls; 20 patients with FMF and 15 healthy controls were enrolled in the study. The erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP), fibrinogen, and leukocyte levels were determined as markers of APR. Thiobarbituric acid reactive substances (TBARS), conjugated diene, and lipid hydroperoxide levels were measured as markers of lipid peroxidation. Carbonyl group and thiol (T-SH) levels were analyzed to determine the oxidative damage to proteins, and 8-hydroxy-2-deoxyguanosine (8-OHdG) was measured to reflect DNA oxidation. The erythrocyte glutathione (GSH) level, and glutathione peroxidase (GSH-Px), CuZn superoxide dismutase (CuZn SOD), and catalase activities were measured as markers of antioxidant status. Conjugated diene (p < 0.001) and carbonyl group (p < 0.05) levels were significantly higher and GSH-Px activity (p < 0.01) was significantly lower in FMF patients compared with controls. FMF patients in the attack period (n = 8) had significantly higher CRP, ESR, fibrinogen, and leukocyte levels (p < 0.001) than patients in the attack-free period (n = 12). The T-SH level (p < 0.05) was significantly higher and CuZn SOD activity was significantly lower (p < 0.05) in FMF patients in the attack period. The findings revealed upregulated APR during the attack period in FMF patients and enhanced oxidative stress in the FMF patients as compared to controls.
authors.affiliation: Department of Biochemistry, Medical Faculty, Namik Kemal University, Tekirdag, Turkey. savasguzel@yahoo.com
authors.forename: Savas
authors.initials: S
authors.lastname: Guzel
authors.forename: Gulnur
authors.initials: G
authors.lastname: Andican
authors.forename: Arzu
authors.initials: A
authors.lastname: Seven
authors.forename: Mahmure
authors.initials: M
authors.lastname: Aslan
authors.forename: Murat
authors.initials: M
authors.lastname: Bolayirli
authors.forename: Eda Celik
authors.initials: EC
authors.lastname: Guzel
authors.forename: Vedat
authors.initials: V
authors.lastname: Hamuryudan
mesh_terms: D000210:Acute-Phase Reaction / Q000209:etiology* / Q000378:metabolism; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D010505:Familial Mediterranean Fever / Q000150:complications* / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D015415:Biomarkers; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D002097:C-Reactive Protein; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase