title: [Advances in two-photon imaging technology].
abstract: As a new kind of advanced nonlinear imaging approach, two-photon fluorescence microscopy technology is wildly used in the field of live cell and tissue imaging, especially focusing on long-term dynamic three-dimensional cell imaging. This paper firstly presents the principle and characteristic of two-photon fluorescence microscopy. Then, the paper focuses on the three key aspects of the viability of the specimen, sensitivity of detection, as well as the speed of acquisition. In the end, the future prospect of development and application of two-phonon imaging technology are predicted.
authors.affiliation: School of Instrumentation Science and Optoelectronics Engineering, Beijing University of Aeronautics and Astronautics, Beijing 100191, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Xia
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Ming
authors.initials: M
authors.lastname: Shi
mesh_terms: D003952:Diagnostic Imaging; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*