title: Two ways to use imaging: focusing directly on mechanism, or indirectly via behaviour?
abstract: Recent developments in mesoscopic imaging--imaging at the level of tissues and organs, rather than the subcellular or molecular scale--are proving to be powerful for developmental biology. At the same time, these developments are also helping to emphasize an important distinction between two quite different approaches of how imaging is used. In the more traditional approach, images provide a direct insight into how a systems works-suggesting a mechanism or part of a mechanism. However an alternative approach is gaining ground, in which imaging is used to quantify the behaviour of a system, rather than directly assessing the mechanism. In this case the causal relationships of a system are inferred in a more indirect way-by comparing quantitative measurements with mathematical models of the system in question. Although indirect, this approach is powerful for addressing more complex biological systems--especially multiscale problems. It is tempting to distinguish the latter approach with the label 'quantitative biology', but this term only emphasizes the use of numbers, and therefore obscures the more fundamental difference, which is the powerful but indirect nature of the approach. Here I will discuss the distinction between the two imaging approaches, particularly in the context of recent improvements to tissue-level imaging techniques.
authors.affiliation: ICREA Research Professor, EMBL-CRG Systems Biology Program, Centre for Genomic Regulation (CRG), UPF, Dr. Aiguader 88, Barcelona 08003, Spain. james.sharpe@crg.es
authors.forename: James
authors.initials: J
authors.lastname: Sharpe
mesh_terms: D000818:Animals; D015509:Developmental Biology / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological