title: Antibacterial nanofiber materials activated by light.
abstract: Electrospun polymeric nanofiber materials doped with 5,10,15,20-tetraphenylporphyrin (TPP) photosensitizer were prepared from four different polymers and were characterized with microscopic methods, steady-state, and time-resolved fluorescence and absorption spectroscopy. The polymers used included polyurethane Larithane™ (PUR), polystyrene (PS), polycaprolactone (PCL), and polyamide 6 (PA6). The antibacterial activity of all nanofiber materials against E. coli was activated by visible light and it was dependent on oxygen permeability/diffusion coefficients and the diameter of the polymeric nanofibers. This activity is based on oxidation ability of singlet oxygen O₂(¹Δ(g)) that is generated upon irradiation. All tested nanofiber materials exhibited prolonged antibacterial properties, even in the dark after long-duration irradiation. The post-irradiation effect was explained by the photogeneration of H₂O₂, which provided the material with long-lasting antibacterial properties.
authors.affiliation: Faculty of Sciences, Charles University in Prague, 128 43 Prague 2, Czech Republic.
authors.forename: Soňa
authors.initials: S
authors.lastname: Jesenská
authors.forename: Lukáš
authors.initials: L
authors.lastname: Plíštil
authors.forename: Pavel
authors.initials: P
authors.lastname: Kubát
authors.forename: Kamil
authors.initials: K
authors.lastname: Lang
authors.forename: Libuše
authors.initials: L
authors.lastname: Brožová
authors.forename: Stěpán
authors.initials: S
authors.lastname: Popelka
authors.forename: Lórant
authors.initials: L
authors.lastname: Szatmáry
authors.forename: Jiří
authors.initials: J
authors.lastname: Mosinger
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D002209:Caprolactam / Q000031:analogs & derivatives / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D008027:Light; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D016877:Oxidants / Q000737:chemistry; D017319:Photosensitizing Agents / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry
chemical_list: C509964:5,10,15,20-tetraphenylporphyrin; D000900:Anti-Bacterial Agents; D016877:Oxidants; D017319:Photosensitizing Agents; D011091:Polyesters; D011108:Polymers; D011137:Polystyrenes; D011140:Polyurethanes; D011166:Porphyrins; D026082:Singlet Oxygen; C016240:polycaprolactone; C009916:nylon 6; D002209:Caprolactam; D006861:Hydrogen Peroxide