title: Mariprofundus ferrooxydans PV-1 the first genome of a marine Fe(II) oxidizing Zetaproteobacterium.
abstract: Mariprofundus ferrooxydans PV-1 has provided the first genome of the recently discovered Zetaproteobacteria subdivision. Genome analysis reveals a complete TCA cycle, the ability to fix CO(2), carbon-storage proteins and a sugar phosphotransferase system (PTS). The latter could facilitate the transport of carbohydrates across the cell membrane and possibly aid in stalk formation, a matrix composed of exopolymers and/or exopolysaccharides, which is used to store oxidized iron minerals outside the cell. Two-component signal transduction system genes, including histidine kinases, GGDEF domain genes, and response regulators containing CheY-like receivers, are abundant and widely distributed across the genome. Most of these are located in close proximity to genes required for cell division, phosphate uptake and transport, exopolymer and heavy metal secretion, flagellar biosynthesis and pilus assembly suggesting that these functions are highly regulated. Similar to many other motile, microaerophilic bacteria, genes encoding aerotaxis as well as antioxidant functionality (e.g., superoxide dismutases and peroxidases) are predicted to sense and respond to oxygen gradients, as would be required to maintain cellular redox balance in the specialized habitat where M. ferrooxydans resides. Comparative genomics with other Fe(II) oxidizing bacteria residing in freshwater and marine environments revealed similar content, synteny, and amino acid similarity of coding sequences potentially involved in Fe(II) oxidation, signal transduction and response regulation, oxygen sensation and detoxification, and heavy metal resistance. This study has provided novel insights into the molecular nature of Zetaproteobacteria.
authors.affiliation: Geomicrobiology Group, Department of Earth Sciences, University of Southern California, Los Angeles, California, United States of America.
authors.forename: Esther
authors.initials: E
authors.lastname: Singer
authors.forename: David
authors.initials: D
authors.lastname: Emerson
authors.forename: Eric A
authors.initials: EA
authors.lastname: Webb
authors.forename: Roman A
authors.initials: RA
authors.lastname: Barco
authors.forename: J Gijs
authors.initials: JG
authors.lastname: Kuenen
authors.forename: William C
authors.initials: WC
authors.lastname: Nelson
authors.forename: Clara S
authors.initials: CS
authors.lastname: Chan
authors.forename: Luis R
authors.initials: LR
authors.lastname: Comolli
authors.forename: Steve
authors.initials: S
authors.lastname: Ferriera
authors.forename: Justin
authors.initials: J
authors.lastname: Johnson
authors.forename: John F
authors.initials: JF
authors.lastname: Heidelberg
authors.forename: Katrina J
authors.initials: KJ
authors.lastname: Edwards
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism*; D016680:Genome, Bacterial / Q000235:genetics / Q000502:physiology*; D010084:Oxidation-Reduction; D020560:Proteobacteria / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D005296:Ferrous Compounds