title: [Proteomics of the colonic mucosa in sub-healthy  people with shapeless stool].
abstract: OBJECTIVE
  To establish the 2-dimensional electrophoresis (2-DE) map in colonic mucosa in sub-healthy people with shapeless stool and healthy people, to identify the differential proteins by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), and to provide theoretical basis for the pathogenesis of intestinal mucosa in sub-healthy people with shapeless stool.
  
  
  METHODS
  Two-DE was used to separate the total proteins from the intestinal mucosa in sub-healthy people (the sub-health group) with the shapeless stool and healthy volunteers (the control group). ImageMaster 2D Elite soft was applied to analyze the 2-DE images, and the differentially expressed protein spots between the 2 groups were identified by MALDI-TOF-MS, protein bank and information technique.
  
  
  RESULTS
  We analyzed the average maps and obtained 517 protein spots in the sub-healthy group and 535 protein spots in the control group. Between the sub-healthy group and the control group, the mean of 366 protein spots was matched, and the matching rate was 70.79%. Ten differential protein spots were screened by MALDI-TOF-MS, and 8 were identified. Five out of the 8 spots were significantly decreased, while 3 out of the 8 were significantly increased.
  
  
  CONCLUSIONS
  The proteomic expression in colonic mucosa of people with shapeless stool is significantly different from that of healthy people. Eight differential proteins such as aldehyde dehydrogenase 1A1 isoform 1, 3-hydroxy-3-methylglutaryl-coenzyme A synthase 2 (mitochondrial), γ-actin, annexin A5 possibly involve in the pathogenesis of sub-healthy people with shapeless stool.
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008, China. zbycsu@hotmail.com
authors.forename: Baiyun
authors.initials: B
authors.lastname: Zhong
authors.forename: Hui
authors.initials: H
authors.lastname: Deng
authors.forename: Jingzhong
authors.initials: J
authors.lastname: Liao
authors.forename: Yunli
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weimin
authors.initials: W
authors.lastname: Xie
authors.forename: Weidong
authors.initials: W
authors.lastname: Liu
authors.forename: Sisi
authors.initials: S
authors.lastname: Feng
mesh_terms: D000199:Actins / Q000378:metabolism; D000444:Aldehyde Dehydrogenase / Q000378:metabolism; D000080924:Aldehyde Dehydrogenase 1 Family; D017304:Annexin A5 / Q000378:metabolism; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism / Q000503:physiopathology; D004415:Dyspepsia / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D006904:Hydroxymethylglutaryl-CoA Synthase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D050697:Retinal Dehydrogenase
chemical_list: D000199:Actins; D017304:Annexin A5; D011506:Proteins; D020543:Proteome; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; D006904:Hydroxymethylglutaryl-CoA Synthase