title: Microdrilled cartilage defects treated with thrombin-solidified chitosan/blood implant regenerate a more hyaline, stable, and structurally integrated osteochondral unit compared to drilled controls.
abstract: This study analyzed the long-term cartilage and subchondral bone repair of microdrilled defects treated with chitosan glycerol-phosphate/blood implant, using thrombin (Factor IIa) to accelerate in situ solidification. We also evaluated the cartilage repair response to six smaller microdrill holes compared with two larger holes. Bilateral knee trochlear cartilage defects were created in n=8 skeletally mature rabbits, drilled with six proximal 0.5 mm and two distal 0.9 mm holes, then covered with in situ-solidified IIa-implants (treated) or with IIa-alone (control). After 6.5 months of repair, cartilage repair tissues were analyzed by histological scoring and histomorphometry for hyaline matrix characteristics and osseous integration. Subchondral repair bone was analyzed by 3D microcomputed tomography and compared to acute defects (n=6) and intact trochlea (n=8). Implant-treated cartilage repair tissues had higher structural integrity through the entire defect (p=0.02), twofold higher percent staining for glycosaminoglycan (p=0.0004), and ~24% more collagen type II staining over the smaller drill holes (p=0.008) compared with controls. Otherwise, hole diameter had no specific effect on cartilage repair. The subchondral bone plate was partially restored in treated and control defects but less dense than intact trochlea, with evidence of incomplete regeneration of the calcified cartilage layer. More residual drill holes (p=0.054) were detected in control versus treated defects, and control defects with more than 40% residual holes presented abnormally thicker trabeculae compared with treated defects. Low osteoclast numbers after 6.5 months repair suggested that bone was no longer remodeling. The subchondral bone plate surrounding the defects exhibited a significant thickening compared with age-matched intact trochlea. These data suggest that debridement and drilling can lead to long-term subchondral bone changes outside the cartilage defect. Compared with drilled controls, chitosan implants solidified with thrombin elicited a more hyaline and structurally integrated osteochondral unit, features needed for long-term durability.
authors.affiliation: Institute of Biomedical Engineering, École Polytechnique de Montréal, Montréal, Quebec, Canada.
authors.forename: Catherine
authors.initials: C
authors.lastname: Marchand
authors.forename: Gaoping
authors.initials: G
authors.lastname: Chen
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tran-Khanh
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.forename: Michael D
authors.initials: MD
authors.lastname: Buschmann
authors.forename: Caroline D
authors.initials: CD
authors.lastname: Hoemann
mesh_terms: D000818:Animals; D001705:Bioprosthesis; D002356:Cartilage / Q000293:injuries*; D048271:Chitosan / Q000737:chemistry*; D006818:Hyalin / Q000737:chemistry*; D016348:Osseointegration; D011817:Rabbits; D013917:Thrombin / Q000737:chemistry*; D013997:Time Factors; D014132:Trachea / Q000293:injuries*
chemical_list: D048271:Chitosan; D013917:Thrombin