title: [Effect of merocianina-540 in the fertilizing capacity of sperm correlated with the expression of 3-OH terminal fragments].
abstract: BACKGROUND
  The packaging of heterochromatin during spermatogenesis has been correlated with the expression of residual apoptotic bodies (which stain with merocyanine A) that will impact on sperm function in the fertilization process; as well as the joint expression of the transmembrane translocation phosphatidyl serine and oligonucleosomes.
  
  
  OBJECTIVE
  To evaluate the expression of bodies stained with merocyanine in the functional processes of sperm and their level of agreement with apoptotic Annexin V and TUNEL biomarkers.
  
  
  METHODS
  We performed a prospective, cross, including 11,000 cells belonging to semen samples from infertile men, were evaluated according to WHO criteria (1999), bounded by the lines of Tygerberg. The biomolecular transformation processing of the membrane and the expression of oligonucleosomes in the terminal cascade of apoptosis were quantified by cytometry flow, using an argon lasser as a reading source of 480 nm, discriminating the degree of cellularity, both negative and positive for each indicators.
  
  
  RESULTS
  Because of the study design was found low quantification in semen parameters, motility, morphology and sperm concentration. The average expression of cells [DNA-PI(+) / dUTP-FITC(+)] (quantification of TUNEL) and [Annexin-V(+) / PI(-)] was 36.5 +/- 17.4% and 31.2 +/- 17.4%, respectively. By comparing the expression of TUNEL without the effect of M540 bodies (36.3 +/- 1.7% vs. 36 +/- 1.7%) a significant difference was not determined.
  
  
  CONCLUSIONS
  This study shows that there is a remnant of the primary processes of spermiation, which can take an important role in apoptotic and functional processes of the sperm. However, its expression does not affect measurement of biomarkers of apoptosis seminal, whose determination changed the diagnosis and functional perception of reproductive parameters in the sperm.
authors.affiliation: Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes. barrosog@me.com
authors.forename: Gerardo
authors.initials: G
authors.lastname: Barroso-Villa
authors.forename: Alinne
authors.initials: A
authors.lastname: Colin-Valenzuela
authors.forename: Nardhy
authors.initials: N
authors.lastname: Gómez-López
authors.forename: Mauricio
authors.initials: M
authors.lastname: Velázquez-Magaña
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D016477:Artifacts; D001363:Awards and Prizes; D003430:Cross-Sectional Studies; D053938:DNA Fragmentation; D005189:False Positive Reactions; D005306:Fertilization / Q000187:drug effects*; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000032:analysis / Q000494:pharmacology*; D005470:Fluorometry; D006176:Gynecology; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods*; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008800:Mexico; D009707:Nucleosomes / Q000187:drug effects* / Q000648:ultrastructure; D009774:Obstetrics; D010718:Phosphatidylserines / Q000378:metabolism; D011446:Prospective Studies; D011744:Pyrimidinones / Q000032:analysis / Q000494:pharmacology*; D013081:Sperm Motility; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D013194:Staining and Labeling
chemical_list: D017304:Annexin A5; D005456:Fluorescent Dyes; D006570:Heterochromatin; D008563:Membrane Lipids; D009707:Nucleosomes; D010718:Phosphatidylserines; D011744:Pyrimidinones; C003954:merocyanine dye; D016650:Fluorescein-5-isothiocyanate