title: Aldosterone increases kidney tubule cell oxidants through calcium-mediated activation of NADPH oxidase and nitric oxide synthase.
abstract: Chronic hyperaldosteronism has been associated with an increased cancer risk. We recently showed that aldosterone causes an increase in cell oxidants, DNA damage, and NF-κB activation. This study investigated the mechanisms underlying aldosterone-induced increase in cell oxidants in kidney tubule cells. Aldosterone caused an increase in both reactive oxygen and reactive nitrogen (RNS) species. The involvement of the activation of NADPH oxidase in the increase in cellular oxidants was demonstrated by the inhibitory action of the NADPH oxidase inhibitors DPI, apocynin, and VAS2870 and by the migration of the p47 subunit to the membrane. NADPH oxidase activation occurred as a consequence of an increase in cellular calcium levels and was mediated by protein kinase C. The prevention of RNS increase by BAPTA-AM, W-7, and L-NAME indicates a calcium-calmodulin activation of NOS. A similar pattern of effects of the NADPH oxidase and NOS inhibitors was observed for aldosterone-induced DNA damage and NF-κB activation, both central to the pathogenesis of chronic aldosteronism. In summary, this paper demonstrates that aldosterone, via the mineralocorticoid receptor, causes an increase in kidney cell oxidants, DNA damage, and NF-κB activation through a calcium-mediated activation of NADPH oxidase and NOS. Therapies targeting calcium, NOS, and NADPH oxidase could prevent the adverse effects of hyperaldosteronism on kidney function as well as its potential oncogenic action.
authors.affiliation: Department of Toxicology, University of Würzburg, Würzburg, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Queisser
authors.forename: Nicole
authors.initials: N
authors.lastname: Schupp
authors.forename: Helga
authors.initials: H
authors.lastname: Stopper
authors.forename: Reinhard
authors.initials: R
authors.lastname: Schinzel
authors.forename: Patricia I
authors.initials: PI
authors.lastname: Oteiza
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004249:DNA Damage; D004285:Dogs; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D016877:Oxidants / Q000378:metabolism*; D026361:Reactive Nitrogen Species / Q000378:metabolism; D013552:Swine
chemical_list: D016328:NF-kappa B; D016877:Oxidants; D026361:Reactive Nitrogen Species; D000450:Aldosterone; D019001:Nitric Oxide Synthase; D019255:NADPH Oxidases; D002118:Calcium