title: Decreased cytochrome c oxidase subunit VIIa in aged rat heart mitochondria: immunocytochemistry.
abstract: Aging decreases oxidative phosphorylation through cytochrome oxidase (COX) in cardiac interfibrillar mitochondria (IFM) in 24-month old (aged) rats compared to 6-month old adult Fischer 344 rats, whereas subsarcolemmal mitochondria (SSM) located beneath the plasma membrane remain unaffected. Immunoelectron microscopy (IEM) reveals in aged rats a 25% reduction in cardiac COX subunit VIIa in cardiac IFM, but not in SSM. In contrast, the content of subunit IV remains unchanged in both SSM and IFM, irrespective of age. These subunits are localized mainly on cristae membranes. In contrast, semi-quantitative immunoblotting, which detects denatured protein, indicates that the content of COX VIIa is similar in IFM and SSM from both aged and adult hearts. IEM provides a sensitive method for precise localizing and quantifying specific mitochondrial proteins. The lack of immunoreaction of COX VIIa subunit by IEM in aged IFM is not explained by a reduction in protein, but rather by a masking phenomenon or by an in situ change in protein structure affecting COX activity.
authors.affiliation: Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA. hisashi.fujioka@case.edu
authors.forename: Hisashi
authors.initials: H
authors.lastname: Fujioka
authors.forename: Shadi
authors.initials: S
authors.lastname: Moghaddas
authors.forename: Deborah G
authors.initials: DG
authors.lastname: Murdock
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Lesnefsky
authors.forename: Bernard
authors.initials: B
authors.lastname: Tandler
authors.forename: Charles L
authors.initials: CL
authors.lastname: Hoppel
mesh_terms: D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D015536:Down-Regulation; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000648:ultrastructure; D010085:Oxidative Phosphorylation; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D003576:Electron Transport Complex IV