title: A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas.
abstract: Plant-specific DNA-binding transcription factors with one finger (Dof) perform important roles in several biological processes. A yeast one-hybrid cDNA library of Jatropha curcas was used to identify Dof-type transcription factors. JcDof3, isolated from the library as a full-length cDNA, encoded a protein of 518 amino acids and contained a highly conserved Dof domain. Yeast one-hybrid systems and subcellular localization assays confirmed that JcDof3 was a typical transcription factor. In contrast to arrhythmic expression at basal level in etiolated cotyledons under continuous dark conditions, the circadian oscillations of JcDof3 transcripts were observed under long day, short day or continuous light regimes. A phylogenetic analysis showed that JcDof3 was clustered into the same clade with CYCLING DOF FACTOR (CDF), which interacts with F-box protein to regulate photoperiodic flowering. Moreover, a yeast two-hybrid assay showed that JcDof3 also interacted with F-box proteins. Our results suggest that JcDof3 is a circadian clock regulated gene, and might be involved in the flowering time regulation of J. curcas.
authors.affiliation: Institute of Botany, the Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Yang
authors.forename: Wen-Peng
authors.initials: WP
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.forename: Shi-Hua
authors.initials: SH
authors.lastname: Shen
mesh_terms: D000595:Amino Acid Sequence; D056804:Biofuels; D057906:Circadian Clocks; D018744:DNA, Plant / Q000378:metabolism; D035264:Flowers / Q000502:physiology*; D031286:Jatropha / Q000235:genetics*; D008969:Molecular Sequence Data; D017440:Photoperiod; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques
chemical_list: D056804:Biofuels; D018744:DNA, Plant; D014157:Transcription Factors