title: Bioactive C15 acetogenins from the red alga Laurencia obtusa.
abstract: The petroleum ether extract of the red alga Laurencia obtusa afforded three new C(15) acetogenins (cyclic ether enyne): (12Z)-cis-maneonene-D (1), (12E)-cis-maneonene-E (2), and (12Z)-trans-maneonene-C (3), along with one known cis-maneonene-A (4). Blood neutrophils were prepared, cultured, and incubated for 24, 48, and 72 h in medium with and without isolated compounds. Blood neutrophils were prepared, cultured, and incubated for 24, 48 and 72 h in medium with and without the isolated compounds. Both morphology and DNA fragmentation methods assessed the percentage of neutrophils apoptosis in each culture. In the present study, several observations have been made concerning the apoptosis-inducing or inhibiting effect of 1 and 2. Both compounds had no inhibition of apoptosis but apoptosis was enhanced significantly by aging. However, 1 stimulated apoptosis of normal only at the initial 24 h. After that there was no significant difference in apoptosis with or without compound 1, while 2 stimulated apoptosis at all the times. The apoptosis induced by these two compounds was demonstrated by DNA fragmentation assay and microscopic observation. These observations suggest that compounds 1 and 2 may be involved in regulation of programmed death in the initiation and propagation of inflammatory responses.
authors.affiliation: Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, KSA, Saudi Arabia. snayyad2@yahoo.com
authors.forename: Seif-Eldin Nasr
authors.initials: SE
authors.lastname: Ayyad
authors.forename: Khalid Omar
authors.initials: KO
authors.lastname: Al-Footy
authors.forename: Walied Mohamed
authors.initials: WM
authors.lastname: Alarif
authors.forename: Tariq Rashad
authors.initials: TR
authors.lastname: Sobahi
authors.forename: Salim Ahmed
authors.initials: SA
authors.lastname: Bassaif
authors.forename: Mohamed Saleh
authors.initials: MS
authors.lastname: Makki
authors.forename: Abdullah Mohamed
authors.initials: AM
authors.lastname: Asiri
authors.forename: Amani Yahya
authors.initials: AY
authors.lastname: Al  Halwani
authors.forename: Amani Yahya
authors.initials: AY
authors.lastname: Al Halawani
authors.forename: Adel Farid
authors.initials: AF
authors.lastname: Badria
authors.forename: Farid Abd Al-raheem
authors.initials: FA
authors.lastname: Badria
mesh_terms: D054378:Acetogenins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D004988:Ethers, Cyclic / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D044722:Laurencia; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013997:Time Factors
chemical_list: D054378:Acetogenins; D004988:Ethers, Cyclic; D018836:Inflammation Mediators; D010936:Plant Extracts