title: The effect of IGF-1 and FSH on the in vitro development of caprine secondary follicles and on the IGF-1, IGFR-I and FSHR mRNA levels.
abstract: This study verified the in vitro effects of IGF-1, FSH or both on caprine preantral follicle development and mRNA levels encoding IGF-1, IGFR-1 and FSHR. Secondary follicles were cultured for six days with FSH, IGF-1 or IGF-1+FSH. The results showed that IGF-1 and/or FSH addition did not influence follicular development for six days. The interaction between IGF-1 and FSH increased the mRNA levels of IGF-1 and FSHR, and FSH increased the expression of the IGFR-1 mRNA. Thus, IGF-1 and/or FSH increased IGF-1, IGFR-1 and FSHR mRNA levels in in vitro cultured caprine secondary follicles, but they did not influence their development after six days of in vitro culture.
authors.affiliation: LAMOFOPA, Universidade Estadual do Ceará, Avenida Paranjana, 1700, Itaperi, CEP: 60740-930, Fortaleza, CE, Brazil. ivinabrito@yahoo.com.br
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.forename: M V A
authors.initials: MV
authors.lastname: Saraiva
authors.forename: V R
authors.initials: VR
authors.lastname: Araújo
authors.forename: J J H
authors.initials: JJ
authors.lastname: Celestino
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.forename: I M T
authors.initials: IM
authors.lastname: Lima
authors.forename: R
authors.initials: R
authors.lastname: Van den Hurk
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000502:physiology; D006041:Goats / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017526:Receptor, IGF Type 1 / Q000235:genetics / Q000378:metabolism*; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011962:Receptors, FSH; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone; D017526:Receptor, IGF Type 1