title: Nano LC-MS/MS: a robust setup for proteomic analysis.
abstract: Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel electrophoresis). Technical challenges, associated with low flow rates of the chromatographic separation, make this technology still difficult to run routinely. Here, we describe a nano LC-MS/MS setup that allows several weeks of continuous operation for the analysis of peptides derived by enzymatic digestion of either purified proteins or moderately complex protein mixtures.
authors.affiliation: Laboratory of Proteomics and Mass Spectrometry, Department of Experimental and Clinical Medicine, "Magna Græcia" University of Catanzaro, Catanzaro, Italy. gaspari@unicz.it
authors.forename: Marco
authors.initials: M
authors.lastname: Gaspari
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cuda
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D016622:Silver Staining; D053719:Tandem Mass Spectrometry / Q000379:methods*