title: Engineering cyclic amidases for non-natural amino acid synthesis.
abstract: Hydantoinases/dihydropyrimidinases are important biotechnological enzymes involved in the production of α- and β-amino acids. Their isolation from new sources with different substrate specificities, improved activity, enantioselectivity, or higher stability continues to be of great industrial interest. Here, we provide a detailed description of how to produce high quantities of the recombinant hydantoinase/dihydropyrimidinase enzyme from Sinorhizobium meliloti CECT4114 (SmeDhp). Several techniques are combined to obtain this goal, from cloning to activity measurement by HPLC.
authors.affiliation: Departamento de Química-Física, Bioquímica y Química Inorgánica, Universidad de Almería, Almería, Spain. fjheras@ual.es
authors.forename: Francisco Javier Las
authors.initials: FJ
authors.lastname: Heras-Vázquez
authors.forename: Josefa María
authors.initials: JM
authors.lastname: Clemente-Jiménez
authors.forename: Sergio
authors.initials: S
authors.lastname: Martínez-Rodríguez
authors.forename: Felipe
authors.initials: F
authors.lastname: Rodríguez-Vico
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000596:Amino Acids / Q000096:biosynthesis*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003500:Cyclization; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D015202:Protein Engineering
chemical_list: D000596:Amino Acids; D017931:DNA Primers; D000581:Amidohydrolases