title: [Effect of willed movement therapy on GFAP and SYP expression in rats with cerebral ischemia-reperfusion].
abstract: OBJECTIVE
  To determine the effect of willed movement on the expression of glial fibrillary acidic protein (GFAP) and synaptophysin (SYP) in adult rats with cerebral ischemia-reperfusion, and explore the mechanism of willed movement in promoting nerve repair and regeneration.
  
  
  METHODS
  Adult rat models of cerebral ischemia-reperfusion injury were established by middle cerebral artery occlusion (MCAO) for 2 h followed by a 24-h reperfusion. The models were then divided randomly into 3 groups, namely the model group, environmental modification (EM) group, and willed movement (WM) group. In each group, neurological deficits were evaluated at 3, 7 and 15 days after reperfusion. Immunohistochemistry and immunofluorescence assay were employed to examine the expression of GFAP and SYP in the brain tissue near the ischemic foci.
  
  
  RESULTS
  The rats in WM group showed lessened neurological deficits at 15 days and lowered expression of GFAP and SYP at 7 and 15 days after reperfusion compared with the model and EM groups (P<0.05). No significant difference was found in the expression of GFAP or SYP between the model group and EM group at any time points.
  
  
  CONCLUSIONS
  Willed movement can promote the functional recovery of neurological deficits following cerebral ischemia-reperfusion probably in relation to enhanced GFAP and SYP expressions in the ischemic brain tissues.
authors.affiliation: Department of Neurology, Central South University, Changsha, China. Hou0718@xy3yy.com
authors.forename: De-ren
authors.initials: DR
authors.lastname: Hou
authors.forename: Shadike
authors.initials: S
authors.lastname: Shawuti
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Liu
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Zhu
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Deng
authors.forename: Zhong-yang
authors.initials: ZY
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Yuan-xin
authors.initials: YX
authors.lastname: Liu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy; D004195:Disease Models, Animal; D005081:Exercise Therapy / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy*; D016708:Synaptophysin / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D016708:Synaptophysin; C569016:Syp protein, rat