title: Functional and phenotypical alterations of polymorphonuclear cells in Sézary syndrome patients.
abstract: Sézary syndrome (SS), the leukemic variant of cutaneous T-cell lymphoma (CTCL), has a poor prognosis and infections represent the most frequent cause of death. Polymorphonucleate granulocytes (PMNs) constitute an essential part of the innate immune system: their phagocytic and killing activity against pathogens is mediated by the interactions between Toll-like receptors (TLRs) and the Pathogen-associated molecular patterns (PAMPs). The aim of this study was to investigate PMN functional activity and phenotype in SS patients and their correlation with the onset of infectious complications. This prospective study enrolled 18 consecutive SS patients; PMN functional activity was evaluated by phagocytosis and intracellular killing tests towards Klebsiella pneumoniae. Flow-cytometry was applied to analyze PMN phenotype. PMNs from SS patients displayed a reduced phagocytic activity and intracellular killing against K. pneumoniae at 30 min and 60 min, more pronounced in SS patients with recurrent infections. CD11b and CD66b median fluorescence intensity (MFI) was significantly higher in SS than in healthy subjects, whereas CD62L MFI was decreased. No significant differences in TLR2, 4, 8 and 9 percentage expression or MFI were found. An increased TLR5 percentage expression was documented. The impairment in PMN functional activities in SS could favour the immune-suppression and raise infection risk.
authors.affiliation: Department of Dermatology, Biomedical Sciences and Human Oncology, Turin University, Via Cherasco 23, 10126 Turin, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Fierro
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Cuffini
authors.forename: Mauro
authors.initials: M
authors.lastname: Novelli
authors.forename: Giuliana
authors.initials: G
authors.lastname: Banche
authors.forename: Valeria
authors.initials: V
authors.lastname: Allizond
authors.forename: Alessandra
authors.initials: A
authors.lastname: Comessatti
authors.forename: Matteo
authors.initials: M
authors.lastname: Brizio
authors.forename: Daniela
authors.initials: D
authors.lastname: Scalas
authors.forename: Chiara
authors.initials: C
authors.lastname: Merlino
authors.forename: Pietro
authors.initials: P
authors.lastname: Quaglino
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bernengo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007711:Klebsiella pneumoniae / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000473:pathology* / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology; D010641:Phenotype; D011446:Prospective Studies; D012751:Sezary Syndrome / Q000276:immunology / Q000401:mortality / Q000473:pathology* / Q000503:physiopathology*; D051198:Toll-Like Receptor 5 / Q000378:metabolism; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D051198:Toll-Like Receptor 5; D051193:Toll-Like Receptors