title: The role of 14-3-3β in transcriptional activation of estrogen receptor α and its involvement in proliferation of breast cancer cells.
abstract: The estrogen receptor (ER) functions as a transcription factor that mediates the effects of estrogen. ERα, which plays a crucial role in the development and progression of breast cancer, is activated by estrogen binding, leading to receptor phosphorylation, dimerization, and recruitment of co-activators and chaperons to the estrogen-bound receptor complex. The 14-3-3 proteins bind to target proteins via phosphorylation and influence many cellular events by altering their subcellular localization or acting as a chaperone. However, regulation of ERα expression and transactivation by the 14-3-3 proteins has not been reported. We demonstrate that 14-3-3β functions as a positive regulator of ERα through a direct protein-protein interaction in an estrogen-dependent manner. Ectopic expression of 14-3-3β stimulated ERα-mediated transcriptional activity in MCF-7 breast cancer cells. Enhanced ERα transcriptional activity due to 14-3-3β increased the expressions of the endogenous ERα target genes, leading to proliferation of breast cancer cells. We suggest that 14-3-3β has oncogenic potential in breast cancer via binding to ERα and activation of the transcriptional activity of ERα.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, South Korea.
authors.forename: Yoonseo
authors.initials: Y
authors.lastname: Kim
authors.forename: Hyungjin
authors.initials: H
authors.lastname: Kim
authors.forename: Sung-Wuk
authors.initials: SW
authors.lastname: Jang
authors.forename: Jesang
authors.initials: J
authors.lastname: Ko
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015533:Transcriptional Activation
chemical_list: D048948:14-3-3 Proteins; D047628:Estrogen Receptor alpha