title: Production of methionine γ- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene.
abstract: The production of antileukemic enzyme methionine γ-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine γ- liyase production. Methionine γ- liyase production by Citrobacter freundii and its vgb(-) and vgb(+) bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30 °C in a time-course manner. The vgb(+) and especially the carbon type had a dramatic effect on methionine γ- liyase production. The vgb(+) strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb(-) strain, respectively.
authors.affiliation: Department of Biology, Faculty of Art and Science, Inonu University, Malatya 44280, Turkey. huseyin.kahraman@inonu.edu.tr
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kahraman
authors.forename: Emel
authors.initials: E
authors.lastname: Aytan
authors.forename: Ash Giray
authors.initials: AG
authors.lastname: Kurt
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002244:Carbon / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000096:biosynthesis* / Q000235:genetics; D016971:Citrobacter freundii / Q000201:enzymology* / Q000235:genetics*; D005285:Fermentation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D002244:Carbon; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase