title: Angiogenesis in oral lichen planus: an in vivo and immunohistological evaluation.
abstract: Oral lichen planus (OLP) is an autoimmune disease with an inflammatory pathogenesis. The angiogenetic phenomenon is a mechanism at the base of the pathogenesis of chronic inflammatory processes. The aim of this research is to evaluate the angiogenetic phenomenon, comparing an in vitro method with an in vivo one. Thirty OLP patients and 30 healthy subjects were enrolled in the study. Immunohistochemical analysis of the vascular-endothelial growth factor (VEGF) and vascular-endothelial adhesion molecules were carried out by the means of primary antibodies and anti-CD34, anti-VEGF, anti-CD106 antigen (VCAM-1) and anti-CD54 antigen (ICAM-1). Capillary density and others capillaroscopic parameters were tested in vivo using oral videocapillaroscopy. The results reveal the presence of a significant angiogenesis in OLP patients through the immunoexpression of VEGF, CD34, CD106 and CD54 (p < 0.001). Capillaroscopic analysis demonstrates significant value for the following parameters: density, tortuosity, loop diameter, afferent and efferent capillary loop diameter. The in vivo and in vitro investigation in OLP reveals a significant angiogenesis.
authors.affiliation: Department of Oral Sciences "G. Messina", University of Palermo, 129 90127, Palermo, Italy. scardina@odonto.unipa.it
authors.forename: Giuseppe Alessandro
authors.initials: GA
authors.lastname: Scardina
authors.forename: Alessia
authors.initials: A
authors.lastname: Ruggieri
authors.forename: Emiliano
authors.initials: E
authors.lastname: Maresi
authors.forename: Pietro
authors.initials: P
authors.lastname: Messina
mesh_terms: D002196:Capillaries / Q000276:immunology / Q000473:pathology; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017676:Lichen Planus, Oral / Q000276:immunology / Q000473:pathology; D008297:Male; D020678:Microscopic Angioscopy / Q000379:methods; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000276:immunology / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; D042461:Vascular Endothelial Growth Factor A