title: Affinity ligands from chemical combinatorial libraries.
abstract: The above discussion provides examples of how to utilize the possibilities arising from different scenarios, related to the level of information available, to identify low molecular weight organic molecule affinity ligands to target proteins. In Table 10.1 the different published results are summarized in terms of the structure of the ligand, the target protein, and a reference to the relevant publication. Common to all reported cases of small molecule affinity ligands is a considerably lower selectivity and affinity compared to natural protein ligands. This lower affinity has to be compensated with more thorough work in the optimization of binding and elution conditions to obtain significant recoveries and purification factors.
authors.affiliation: GE Healthcare Bio-Sciences AB, SE-751 84 Uppsala, Sweden.
authors.forename: Enrique
authors.initials: E
authors.lastname: Carredano
authors.forename: Herbert
authors.initials: H
authors.lastname: Baumann
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D054852:Small Molecule Libraries
chemical_list: D008024:Ligands; D009930:Organic Chemicals; D011506:Proteins; D054852:Small Molecule Libraries