title: L-amino acid oxidase-induced apoptosis in filamentous Botrytis cinerea.
abstract: As opposed to single-cell yeast and mammalian cell lines, apoptosis has not been greatly investigated in filamentous fungi because antibodies to the relevant fungal apoptosis-related proteins are not available commercially and because multicellular organisms cannot be studied using flow cytometry. Here we demonstrate how antibodies from a nonfungal source could be used to investigate this pathway. We show that apoptosis in the filamentous fungus Botrytis cinerea is triggered by the mitochondria-mediated caspase pathway, with release of the apoptotic factors cytochrome c, caspase 3, and caspase 9, on treatment with Trichoderma harzianum-derived L-amino acid oxidase.
authors.affiliation: Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, Taiwan, ROC.
authors.forename: Chi-Hua
authors.initials: CH
authors.lastname: Cheng
authors.forename: Chia-Ann
authors.initials: CA
authors.lastname: Yang
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Liu
authors.forename: Chaur-Tsuen
authors.initials: CT
authors.lastname: Lo
authors.forename: Kou-Cheng
authors.initials: KC
authors.lastname: Peng
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D020171:Botrytis / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism / Q000494:pharmacology; D050830:L-Amino Acid Oxidase / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology
chemical_list: D005656:Fungal Proteins; D045304:Cytochromes c; D050830:L-Amino Acid Oxidase; D053148:Caspase 3; D053453:Caspase 9