title: A novel targeted multi-functional fusion protein possesses inhibitory activities against bacteria, thrombin and platelet aggregation.
abstract: In the present study, we designed a novel targeted multi-functional fusion protein LHAD composed of LL-37, FXa recognition peptide, hirudin-12-residue, AAP, and RGD peptide. It was expressed in the Pichia pastoris GS115 strain and purified by affinity chromatography. The in vitro studies suggested that the novel designed protein exhibited antibacterial activity, anti-platelet aggregation and anti-thrombin activities. Moreover, the capability of anti-thrombin was significantly increased compared to that of natural hirudin. Our study may provide a potential approach to design multi-functional drugs for the prevention and management of thrombosis.
authors.affiliation: Bioengineering College, Chongqing University, Chongqing, People's Republic of China.
authors.forename: Jiange
authors.initials: J
authors.lastname: Gao
authors.forename: Zongli
authors.initials: Z
authors.lastname: Hu
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhao
authors.forename: Guanglei
authors.initials: G
authors.lastname: Liu
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Ren
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D000991:Antithrombins / Q000378:metabolism / Q000494:pharmacology*; D015195:Drug Design; D015870:Gene Expression; D006801:Humans; D010455:Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors; D013927:Thrombosis / Q000188:drug therapy
chemical_list: D000991:Antithrombins; D010455:Peptides; D010975:Platelet Aggregation Inhibitors; D011993:Recombinant Fusion Proteins; D013917:Thrombin