title: Optimization of the mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-).
abstract: The mated fermentation process for the production of lycopene by Blakeslea trispora NRRL 2895 (+) and NRRL 2896 (-) was systematically optimized in shake flasks. The ratio of the (+) to (-) strains, the lycopene cyclase inhibitors piperidine and creatinine, the trisporic acid structural analog abscisic acid, the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) precursor leucine, and the mevalonate kinase enhancer penicillin were all identified as key factors affecting lycopene biosynthesis. With an optimal ratio of 5:1 for the (+) to (-) strains and the addition of 6 g/L creatinine on day 3, the highest lycopene production was 98.1 ± 15.5 mg/L. Based on the above result, the addition of 0.1 g/L penicillin on day 4, 150 μmol/L abscisic acid on day 3 or 0.5 g/L leucine on day 4 enhanced lycopene production to 119.7 ± 17.2, 120.6 ± 12.3 and 135.2 ± 7.0 mg/L, respectively. Finally, an integrated strategy by combining the above key factors was developed, and the highest lycopene production of 156.2 ± 15.4 mg/L was obtained, which was enhanced by 134.9% comparing with its production of 66.5 ± 3.6 mg/L before the optimization process of this work. The results obtained in this study may be useful for large-scale industrial lycopene production.
authors.affiliation: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, China.
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Xiu-Ji
authors.initials: XJ
authors.lastname: Liu
authors.forename: Rui-Sang
authors.initials: RS
authors.lastname: Liu
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Tang
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis* / Q000302:isolation & purification; D018920:Coculture Techniques / Q000379:methods*; D003198:Computer Simulation; D005285:Fermentation; D000077276:Lycopene; D008954:Models, Biological; D013045:Species Specificity; D013149:Spiroplasma / Q000145:classification* / Q000378:metabolism*
chemical_list: D002338:Carotenoids; D000077276:Lycopene