title: Adenylyl and guanylyl cyclase assays.
abstract: This unit presents two basic protocols to determine adenylyl cyclase and guanylyl cyclase activity in tissue and cell homogenates, permeabilized cells, or subcellular fractions. Each method is divided into two parts: the enzyme reaction that causes the formation of the labeled cyclic nucleotide, and the separation of cyclic nucleotide products from unreacted nucleotide triphosphates and metabolites using Dowex 50 resin and aluminum oxide chromatographies. In the case of guanylyl cyclase, alternative separation protocols are also provided. Additionally, protocols are provided that describe preparation of both the columns used in the assays and the tissue or cells to be assayed.
authors.affiliation: College of Medicine, University of South Alabama, Mobile, AL, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Kelly
authors.forename: Troy
authors.initials: T
authors.lastname: Stevens
authors.forename: W Joseph
authors.initials: WJ
authors.lastname: Thompson
authors.forename: Roland
authors.initials: R
authors.lastname: Seifert
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D006162:Guanylate Cyclase / Q000032:analysis* / Q000378:metabolism; D006801:Humans
chemical_list: D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase