title: Antiulcer activity of anthocyanins from Rubus coreanus via association with regulation of the activity of matrix metalloproteinase-2.
abstract: Anthocyanins were extracted from the fruits of Rubus coreanus. Whether their antioxidant properties and antiulcer activity in gastric ulceration have been accompanied by the activation of matrix metalloproteainse-2 (MMP-2) was investigated. To assess the effect of anthocyanins on gastric ulcer, the rats were administered with anthocyanins (20, 50, and 80 mg/kg of body weight) before treatment with naproxen (80 mg/kg of body weight) to induce gastric ulceration. Lipid peroxidation and the activities of radical scavenging enzymes such as catalase, superoxide dismutase, and glutathione peroxidase were determined. The MMP-2 level was tested by zymography and Western blot. Anthocyanins of R. coreanus exhibit possible antiulcer activity in acute ulcer in a rat model by preventing lipid peroxidation and a significant increase in the activities of antioxidant enzymes such as catalase, superoxide dismutase, and glutathione peroxidase. Also, anthocyanins induce activation of MMP-2 and attenuate the activity of the proinflammatory molecules, such as tumor necrosis factor-α and interleukin-1β.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 136-701, Republic of Korea.
authors.forename: Sun-Joong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Bum-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Yoo
authors.forename: Hyo Ihl
authors.initials: HI
authors.lastname: Chang
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry*; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts; D020778:Matrix Metalloproteinase 2