title: [The preparation of rabbit antiserum against gekko japonicus Hoxc10  and it's property identification].
abstract: OBJECTIVE
  Prepare the rabbit antiserum against gecko japonicus Hoxc10 and to identify its properties.
  
  
  METHODS
  Prokaryotic expression vector of g-Hoxc10 were constructed and then transform into E.coli (BL21). To make GST-g-Hoxc10 fusion protein in E.coli (BL21) under the optimized induction of Isopropyl β-D-1-thiogalactopyranoside(IPTG). The recombination proteins were purified using affinity chromatography. The purified fusion protein was inoculated into adult rabbits to develop antiserum. Western blot and immunohistochemistry staining were then performed to evaluate the feature of the prepared antiserum.
  
  
  RESULTS
  Prokaryotic expression vectors of g-Hoxc10 were successfully constructed. The soluble recombinant protein was highly expressed in E.coli BL21 and inoculated into adult rabbits to obtain high titer antiserum. Western blot and immunohistochemistry staining were then performed to evaluate the specificity of the prepared antiserum.
  
  
  CONCLUSIONS
  We successfully amplified and expressed the g-Hoxc10 in E.coli BL21. The purified fusion protein was inoculated into adult rabbits to develop antiserum. The obtained antiserum of g-Hoxc10 showed a high titer against Hoxc10 proteins. The protein and antiserum prepared in this study can be used for further research of the function investigation of Hoxc10.
authors.affiliation: Key Laboratory of Neuroregeneration, Nantong University, Nantong, China.
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Zhou
authors.forename: Ren-fei
authors.initials: RF
authors.lastname: Lu
authors.forename: Qian
authors.initials: Q
authors.lastname: Chen
authors.forename: Yu-dan
authors.initials: YD
authors.lastname: Chu
authors.forename: Xue-qian
authors.initials: XQ
authors.lastname: Wang
authors.forename: Tian-mei
authors.initials: TM
authors.lastname: Qian
authors.forename: Xing-xing
authors.initials: XX
authors.lastname: Gu
mesh_terms: D000818:Animals; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology* / Q000302:isolation & purification; D008116:Lizards; D008297:Male; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins