title: Influence of Pleurotus ostreatus inoculation on wood degradation and fungal colonization.
abstract: The influence of Pleurotus ostreatus inoculation on wood degradation and on fungal community structure was studied. The experiments were performed on an organically poor fly ash deposit covered with a 10 cm layer of beech wood chips inoculated with P. ostreatus isolate ZIM76. Compared to non-inoculated wood chips, inoculation increased the temperatures and relative humidities and, in the first 6 months, accelerated Klason lignin degradation by 9% and also, after 17 months, increased iron translocation into wood chips by 30%. After 6 months, PCR-DGGE showed 22-28 and 13-21 fungal taxa in non-inoculated and P. ostreatus-inoculated beech chips, respectively. The differences in number of taxa and in the fungal community structure (based on Dice coefficient) between non-inoculated and inoculated wood chips diminished with time. The results indicate that the naturally occurring processes of wood degradation are as efficient as those occurring in sites inoculated with P. ostreatus.
authors.affiliation: Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia. barbara.piskur@gozdis.si
authors.forename: Barbara
authors.initials: B
authors.lastname: Piškur
authors.forename: Marko
authors.initials: M
authors.lastname: Bajc
authors.forename: Robert
authors.initials: R
authors.lastname: Robek
authors.forename: Miha
authors.initials: M
authors.lastname: Humar
authors.forename: Iztok
authors.initials: I
authors.lastname: Sinjur
authors.forename: Aleš
authors.initials: A
authors.lastname: Kadunc
authors.forename: Primož
authors.initials: P
authors.lastname: Oven
authors.forename: Gregor
authors.initials: G
authors.lastname: Rep
authors.forename: Samar
authors.initials: S
authors.lastname: Al Sayegh Petkovšek
authors.forename: Hojka
authors.initials: H
authors.lastname: Kraigher
authors.forename: Dušan
authors.initials: D
authors.lastname: Jurc
authors.forename: Franc
authors.initials: F
authors.lastname: Pohleven
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon / Q000032:analysis; D060729:Coal Ash / Q000737:chemistry; D015169:Colony Count, Microbial; D058645:Denaturing Gradient Gel Electrophoresis; D029964:Fagus; D005658:Fungi / Q000235:genetics / Q000254:growth & development*; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000032:analysis; D020076:Pleurotus / Q000254:growth & development / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D013696:Temperature; D014934:Wood / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D060729:Coal Ash; D002244:Carbon; D009584:Nitrogen