title: MiR-143 enhances adipogenic differentiation of 3T3-L1 cells through targeting the coding region of mouse pleiotrophin.
abstract: Adipogenic differentiation of preadipocytes is a complex process regulated by various factors including miRNAs and cytokines. MiR-143 is a well known miRNA that enhances adipogenesis. Pleiotrophin (PTN), a heparin-binding growth factor, plays a negative role in adipogenesis. In this investigation, we demonstrate that PTN is a target gene of miR-143 during adipogenic differentiation in 3T3-L1 preadipocytes. MiR-143 down regulates PTN expression through interaction with a target site of miR-143 in the coding region of mouse PTN. The rare codons upstream of the target site regulate miR143-induced translational knockdown of PTN, which provides more insight into the mechanism of adipogenic differentiation.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Yi
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Xie
authors.forename: Fu
authors.initials: F
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Wu
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.forename: Naihan
authors.initials: N
authors.lastname: Xu
authors.forename: Yaou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D015536:Down-Regulation / Q000502:physiology*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D016366:Open Reading Frames / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D016207:Cytokines; D035683:MicroRNAs; C532884:MIRN143 microRNA, mouse; C066845:pleiotrophin