title: Impact of anaerobiosis strategy and bioreactor geometry on the biochemical response of Clostridium butyricum VPI 1718 during 1,3-propanediol fermentation.
abstract: The impact of anaerobiosis strategy on 1,3-propanediol production during cultivation of Clostridium butyricum VPI 1718 in different size bioreactors was studied. In batch trials with N2 gas infusion, the fermentation was successfully accomplished, regardless of initial glycerol concentration imposed and bioreactor geometry. However, in the absence of N2 continual sparging, significant variations concerning the biochemical response of the strain were observed. Specifically, at 1-L bioreactor, the absence of N2 infusion at high initial glycerol concentration induced lactate dehydrogenase activity and thus lactic acid synthesis, probably due to partial blockage of phosphoroclastic reaction caused by insufficient self-generated anaerobiosis environment. During fed-batch cultivation with continual N2 sparging, the strain produced ∼71 g L(-1) of 1,3-propanediol, whereas under self-generated anaerobiosis, 1,3-propanediol pathway was evidently restricted, as only 30.5 g L(-1) of 1,3-propanediol were finally produced. Apparently, N2 infusion strategy paired with bioreactor geometry can alter the biochemical behavior of the particular strain.
authors.affiliation: Department of Food Science and Technology, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece.
authors.forename: Afroditi
authors.initials: A
authors.lastname: Chatzifragkou
authors.forename: George
authors.initials: G
authors.lastname: Aggelis
authors.forename: Michael
authors.initials: M
authors.lastname: Komaitis
authors.forename: An-Ping
authors.initials: AP
authors.lastname: Zeng
authors.forename: Seraphim
authors.initials: S
authors.lastname: Papanikolaou
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D020148:Butyric Acid / Q000032:analysis; D046972:Clostridium butyricum / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000032:analysis; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D011409:Propylene Glycols / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011409:Propylene Glycols; D020148:Butyric Acid; D019344:Lactic Acid; C041787:1,3-propanediol; D007770:L-Lactate Dehydrogenase; D005990:Glycerol