title: [Self-synchronization of the protein synthesis rhythm in HaCaT cultures of human keratinocytes].
abstract: In cultures of human keratinocytes HaCaT contained in a serum-free medium on glass, a circahoralian rhythm of protein synthesis was found similar to the one in hepatocytes in vitro. The intensity of the synthesis was determined by the inclusion of 3H-leucine corrected for the pool of free marked leucine. Rhythm was studied in washed 1- or 2-day cultures after the change of the medium. The medium conditioned with keratinocytes HaCaT synchronized the rarefied hepatocyte cultures nonsynchronous in the control. Therefore, the keratinocytes liberate synchronizing factors into the medium. A BAPTA-AM chelator of calcium ions eliminates the protein synthesis rhythm both in dense hepatocyte cultures synchronous in the control and in the HaCaT keratinocyte cultures. The effect of the H7 inhibitor of protein kinases was analogous. Thus, both in keratinocytes and hepatocytes, self-synchronization of fluctuations of the intensity of protein synthesis takes place. The mechanism of self-synchronization is the calcium-depending phosphorylation of cell proteins.
authors.forename: V Ia
authors.initials: VIa
authors.lastname: Brodskiĭ
authors.forename: V V
authors.initials: VV
authors.lastname: Terskhikh
authors.forename: A V
authors.initials: AV
authors.lastname: Vasil'ev
authors.forename: N D
authors.initials: ND
authors.lastname: Zvezdina
authors.forename: E A
authors.initials: EA
authors.lastname: Voroteliak
authors.forename: V I
authors.initials: VI
authors.lastname: Fateeva
authors.forename: L A
authors.initials: LA
authors.lastname: Mal'chenko
mesh_terms: D002460:Cell Line; D002614:Chelating Agents / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D017077:Culture Media, Conditioned; D011500:Protein Synthesis Inhibitors; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid