title: HPLC determination of antilipoxygenase activity of a water infusion of Ligustrum vulgare L. leaves and some of its constituents.
abstract: The aim of the study was a HPLC evaluation of the lipoxygenase activity inhibiting activity of a water infusion of Ligustrum vulgare L. leaves and selected isolates from it. The antiradical activity of the water infusion was determined using DPPH, ABTS and FRAP tests. Oleuropein and echinacoside concentrations in the water infusion were determined by HPLC. Water infusion, echinacoside and oleuropein were used for an antilipoxygenase activity assay using lipoxygenase isolated from rat lung cytosol fraction. Activity of 8-LOX, 12-LOX and 15-LOX were monitored through formation of 8-HETE, 12-HETE and 15-HETE, respectively. The water infusion exhibited the highest activity against all lipoxygenases, followed by oleuropein. Echinacoside was ineffective against LOXs in lower concentrations, while higher concentration showed similar inhibition on 8-LOX and 12-LOX. 15-LOX was affected more and the presence of echinacoside remarkably decreased its activity.
authors.affiliation: Department of Pharmacognosy and Botany, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovak Republic. mucaji@fpharm.uniba.sk
authors.forename: Pavel
authors.initials: P
authors.lastname: Mučaji
authors.forename: Anna
authors.initials: A
authors.lastname: Záhradníková
authors.forename: Lýdia
authors.initials: L
authors.lastname: Bezáková
authors.forename: Mária
authors.initials: M
authors.lastname: Cupáková
authors.forename: Drahomíra
authors.initials: D
authors.lastname: Rauová
authors.forename: Milan
authors.initials: M
authors.lastname: Nagy
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D006027:Glycosides / Q000032:analysis / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D057888:Iridoid Glucosides; D039823:Iridoids; D031659:Ligustrum; D008084:Lipoxygenase / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D008168:Lung; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011714:Pyrans / Q000032:analysis / Q000494:pharmacology; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D006027:Glycosides; D006893:Hydroxyeicosatetraenoic Acids; D057888:Iridoid Glucosides; D039823:Iridoids; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; D011714:Pyrans; C002769:oleuropein; C047628:8-hydroxyeicosatetraenoic acid; D008084:Lipoxygenase; C060297:echinacoside