title: Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.
abstract: Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.
authors.affiliation: Department of Early Discovery Biochemistry, Genentech, Inc., South San Francisco, CA, USA. hannoush.rami@gene.com
authors.forename: Rami N
authors.initials: RN
authors.lastname: Hannoush
mesh_terms: D000818:Animals; D001386:Azides / Q000378:metabolism; D001710:Biotin / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D011232:Chemical Precipitation; D057930:Click Chemistry; D004586:Electrophoresis; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D054878:Lipoylation; D019814:Myristic Acid / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D001386:Azides; D005227:Fatty Acids; D012235:Rhodamines; D019814:Myristic Acid; D001710:Biotin