title: Simultaneous measurement of ¹H-¹⁵N and methyl ¹Hm-¹³Cm residual dipolar couplings in large proteins.
abstract: A two-dimensional TROSY-based SIM-(13)C(m)-(1)H(m)/(1)H-(15)N NMR experiment for simultaneous measurements of methyl (1)                         D                         (CH) and backbone amide (1)                         D                         (NH) residual dipolar couplings (RDC) in {U-[(15)N,(2)H]; Ileδ1-[(13)CH(3)]; Leu,Val-[(13)CH(3)/(12)CD(3)]}-labeled samples of large proteins is described. Significant variation in the alignment tensor of the 82-kDa enzyme Malate synthase G is observed as a function of only slight changes in experimental conditions. The SIM-(13)C(m)-(1)H(m)/(1)H-(15)N data sets provide convenient means of establishing the alignment tensor characteristics via the measurement of (1)                         D                         (NH) RDCs in the same protein sample.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Xinli
authors.initials: X
authors.lastname: Liao
authors.forename: Raquel
authors.initials: R
authors.lastname: Godoy-Ruiz
authors.forename: Chenyun
authors.initials: C
authors.lastname: Guo
authors.forename: Vitali
authors.initials: V
authors.lastname: Tugarinov
mesh_terms: D002247:Carbon Isotopes; D007930:Leucine / Q000235:genetics; D008292:Malate Synthase / Q000737:chemistry; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D014633:Valine / Q000235:genetics
chemical_list: D002247:Carbon Isotopes; D011506:Proteins; D008292:Malate Synthase; D007930:Leucine; D014633:Valine