title: Study of the interaction between bovine serum albumin and ZnS quantum dots with spectroscopic techniques.
abstract: The interaction between bovine serum albumin (BSA) and ZnS quantum dots (QDs) was studied by fluorescence, UV-vis spectroscopic techniques. The results showed that the fluorescence of BSA was strongly quenched by ZnS QDs and the quenching mechanism was discussed to be a static quenching procedure, which was proved by quenching rate constant K(q.) The recorded UV-vis data and the fluorescence data quenching by the QDs demonstrated that the interaction between them leads to the formation of QDs-BSA complex. Furthermore, the temperature effects on the structural and spectroscopic properties of individual QDs and protein and their bioconjugates (QDs-BSA) were also researched. It was found that, compared to the monotonically decrease of the individual QDs fluorescence intensity, the temperature dependence of the QDs-BSA emission had a much more complex behavior, highly sensitive to the conformational changes of the protein.
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan 523808, PR China.
authors.forename: Dudu
authors.initials: D
authors.lastname: Wu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D002417:Cattle; D005453:Fluorescence; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D045663:Quantum Dots; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides / Q000378:metabolism*; D013696:Temperature; D014961:X-Ray Diffraction; D017967:Zinc Compounds / Q000378:metabolism*
chemical_list: D013440:Sulfides; D017967:Zinc Compounds; D012710:Serum Albumin, Bovine; C031238:zinc sulfide