title: Structure of the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582: identification of 4-deoxy-D-xylo-hexose as a component of bacterial polysaccharides.
abstract: A novel constituent of bacterial polysaccharides, 4-deoxy-D-xylo-hexose (D-4dxylHex), was found in the major O-specific polysaccharide from the lipopolysaccharide of Pseudomonas fluorescens BIM B-582. D-4dxylHex was isolated in the free state by paper chromatography after full acid hydrolysis of the polysaccharide and identified by GLC-mass spectrometry, 1H and 13C NMR spectroscopy, and specific rotation. It occurs as a lateral substituent in ∼40% of the oligosaccharide repeating units, making the polysaccharide devoid of strict regularity. The structure of the polysaccharide was established by sugar analysis, Smith degradation, and two-dimensional 1H and 13C NMR spectroscopy. In addition, a minor polysaccharide was isolated from the same lipopolysaccharide and found to contain 4-O-methylrhamnose.
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky Prospect, 119991 Moscow, Russian Federation. olga.valueva@gmail.com
authors.forename: Olga A
authors.initials: OA
authors.lastname: Valueva
authors.forename: Dzianis
authors.initials: D
authors.lastname: Rakhuba
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Evelina L
authors.initials: EL
authors.lastname: Zdorovenko
authors.forename: Elena
authors.initials: E
authors.lastname: Kiseleva
authors.forename: Galina
authors.initials: G
authors.lastname: Novik
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D003837:Deoxy Sugars / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000737:chemistry; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011551:Pseudomonas fluorescens / Q000737:chemistry / Q000276:immunology
chemical_list: D003837:Deoxy Sugars; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; C025557:4-deoxy-D-xylo-hexose