title: [Determination of geniposide, crocin and crocetin in different processing products of fructus gardeniae by HPLC-ELSD].
abstract: OBJECTIVE
  To develop a HPLC- ELSD method for determination the contents of geniposide, crocin and crocetin in different processing products of Fructus Gardeniae.
  
  
  METHODS
  The separation was performed in the HyperClone ODS C18 column (250 mm x 4. 6 mm, 5 microm) with linear gradient elution using methanol-water and 0.05% phosphoric acid in water, the flowing rate was 0.8 mL/min, the column temperature was 30 degrees C, and the ELSD parameter was as follow: 70 degrees C as atomization temperature and 2.0 L/min as the gas flowing rate.
  
  
  RESULTS
  The contents of geniposide and crocin in raw, yellowish, carbocoal and scorched Fructus Gardeniae decreased with the deepening of processing degree. However, the content of crocetin in carbocoal and scorched Fructus Gardeniae increased comparing with the raw one.
  
  
  CONCLUSIONS
  This is a simple and credible quality control method, and can be used for the quality control and comprehensive evaluation for different processed products of Fructus Gardeniae.
authors.affiliation: Key Laboratory of Modem Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China. laurayan.125@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhong-Lin
authors.initials: ZL
authors.lastname: Yang
authors.forename: Lei-Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Su-Juan
authors.initials: SJ
authors.lastname: Liu
authors.forename: Xian-Tao
authors.initials: XT
authors.lastname: Zhang
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D032062:Gardenia / Q000737:chemistry*; D039823:Iridoids / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D013678:Technology, Pharmaceutical / Q000379:methods; D014801:Vitamin A / Q000031:analogs & derivatives
chemical_list: D004365:Drugs, Chinese Herbal; D039823:Iridoids; C487773:trans-sodium crocetinate; D014801:Vitamin A; C007835:geniposide; D002338:Carotenoids; C029036:crocin