title: Targeting mucosal immunity in the battle to develop a mastitis vaccine.
abstract: The mucosal immune system encounters antigens that enhance and suppress immune function, and serves as a selective barrier against invading pathogens. The mammary gland not only encounters antigens but also produces a nutrient evolved to protect and enhance mucosal development in the neonate. Efforts to manipulate antibody concentrations in milk to prevent mastitis, an infection of the mammary gland, have been hampered both by complexity and variation in target pathogens and limited knowledge of cellular immunity in the gland. Successful vaccination strategies must overcome the natural processes that regulate types and concentrations of milk antibodies for neonatal development, and enhance cellular immunity. Furthermore, the need to overcome dampening of immunity caused by non-pathogenic encounters to successfully prevent establishment of infection is an additional obstacle in vaccine development at mucosal sites. A significant mastitis pathogen, Staphylococcus aureus, not only resides as a normal flora on a multitude of species, but also causes clinical disease with limited treatment options. Using the bovine model of S. aureus mastitis, researchers can decipher the role of antigen selection and presentation by mammary dendritic cells, enhance development of central and effector memory function, and subsequently target specific memory cells to the mammary gland for successful vaccine development. This brief review provides an overview of adaptive immunity, previous vaccine efforts, current immunological findings relevant to enhancing immune memory, and research technologies that show promise in directing future vaccine efforts to enhance mammary gland immunity and prevent mastitis.
authors.affiliation: Immunology, Human Therapeutic Division, Intrexon Corporation, Germantown, MD, USA.
authors.forename: Mini
authors.initials: M
authors.lastname: Bharathan
authors.forename: Isis K
authors.initials: IK
authors.lastname: Mullarky
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal / Q000276:immunology*; D008413:Mastitis / Q000276:immunology* / Q000517:prevention & control*; D014611:Vaccination