title: Antiophidic solanidane steroidal alkaloids from Solanum campaniforme.
abstract: Three new solanidane alkaloids bearing a 22,23-epoxy ring (1-3) and four known compounds were isolated from leaves of Solanum campaniforme. The structures were determined using spectroscopic techniques, including 1D and 2D NMR, and HRESIMS experiments. The antiophidic activity of the alkaloids was tested against Bothrops pauloensis venom. Compounds 1-3 completely inhibited myotoxicity without inhibiting phospholipase A2 activity of the venom, while hemorrhage and skin necrosis were significantly reduced in the presence of alkaloids 1 and 2.
authors.affiliation: Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, 12.200, Fortaleza-CE, 60.021-970, Brazil.
authors.forename: Maria Conceição M
authors.initials: MC
authors.lastname: Torres
authors.forename: Francisco
authors.initials: F
authors.lastname: das Chagas L Pinto
authors.forename: Raimundo
authors.initials: R
authors.lastname: Braz-Filho
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.forename: Otília Deusdênia L
authors.initials: OD
authors.lastname: Pessoa
authors.forename: Roberta Jeane Bezerra
authors.initials: RJ
authors.lastname: Jorge
authors.forename: Rafael Matos
authors.initials: RM
authors.lastname: Ximenes
authors.forename: Helena Serra Azul
authors.initials: HS
authors.lastname: Monteiro
authors.forename: Janaina Serra Azul
authors.initials: JS
authors.lastname: Monteiro Evangelista
authors.forename: Eduardo B S
authors.initials: EB
authors.lastname: Diz-Filho
authors.forename: Marcos Hikari
authors.initials: MH
authors.lastname: Toyama
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D017837:Bothrops / Q000502:physiology; D001938:Brazil; D003435:Crotalid Venoms / Q000097:blood / Q000378:metabolism / Q000633:toxicity*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018515:Plant Leaves / Q000737:chemistry; D032322:Solanum / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: C568799:22alpha,23alpha-epoxy-solanida-1,4,9-trien-3-one; C568800:22alpha,23alpha-epoxy-solanida-1,4-dien-3-one; C568806:3,9beta-hydroxy-22alpha,23alpha-epoxy-9(10)-seco-solanida-1,3,5(10)-triene; D000470:Alkaloids; D003435:Crotalid Venoms; D013256:Steroids