title: Gene expression profiles are altered in human papillomavirus-16 E6 D25E-expressing cell lines.
abstract: Previously, we have reported that the human papillomavirus (HPV) type 16 E6 D25E is the most prevalent variant in Korean women at high risk for cervical cancers. Several studies have identified an association between the increased frequency of this variant and the elevated risk of cervical intraepithelial neoplasia and invasive cervical carcinoma. To investigate whether the HPV-16 E6 D25E variant might influence cervical cancer progression, we used an oligonucleotide microarray approach to identify transcriptionally altered gene expression patterns in recombinant wild-type E6 or E6 D25E variant-expressing HPV-negative cancer cells. We found that 211 genes were significantly up- or down-regulated (at least 1.5-fold, p < 0.05). We identified 14 genes, nine down-regulated and five up-regulated upon E6 D25E expression, compared with wild-type E6 expression. These results further emphasize the unique biological activity of the HPV-16 E6 D25E variant.
authors.affiliation: Division of AIDS, Center for Immunology and Pathology, Korea National Institute of Health, Seoul, Republic of Korea.
authors.forename: Mi
authors.initials: M
authors.lastname: Jang
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Rhee
authors.forename: Dai-Ho
authors.initials: DH
authors.lastname: Jang
authors.forename: Sung Soon
authors.initials: SS
authors.lastname: Kim
mesh_terms: D000328:Adult; D045744:Cell Line, Tumor; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D005260:Female; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics* / Q000473:pathology / Q000821:virology; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014161:Transduction, Genetic; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins