title: Overexpression of GRP78 protects glial cells from endoplasmic reticulum stress.
abstract: Endoplasmic reticulum (ER) stress induces apoptotic cell death by causing the accumulation of structurally abnormal proteins. The 78-kDa glucose-regulated protein (GRP78) is an ER chaperone that regulates protein folding in the ER and has been suggested to contribute to cell survival. Using the rat C6 glioma cell line and flow cytometry, we assessed GRP78 expression following tunicamycin- and glutamate-induced ER stress. The results showed that GRP78 expression is upregulated following ER stress and has protective effects on injured glial cells. Annexin V and propidium iodide labeling revealed cells transiently expressing GRP78 prior to injury were protected against high-concentrations of tunicamycin and glutamate within 72 h. Our findings support the hypothesis that GRP78 inhibits cell death associated with ER stress.
authors.affiliation: Department of Anatomy and Cellular Biology, Basic Medical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Suyama
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Kou
authors.initials: K
authors.lastname: Sakabe
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Okada
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsuyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kuroiwa
authors.forename: Joji
authors.initials: J
authors.lastname: Mochida
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor / Q000187:drug effects / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005813:Genes, Synthetic; D005910:Glioma / Q000473:pathology; D018698:Glutamic Acid / Q000633:toxicity; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009498:Neurotoxins / Q000633:toxicity; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014415:Tunicamycin / Q000633:toxicity; D000091342:Endoplasmic Reticulum Chaperone BiP
chemical_list: D018698:Glutamic Acid; D006360:Heat-Shock Proteins; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D011993:Recombinant Fusion Proteins; D014415:Tunicamycin; C051684:GRP78 protein, rat; D000091342:Endoplasmic Reticulum Chaperone BiP