title: Cutaneous hypersensitivity test to evaluate phage display anti-tick borne vaccine antigen candidates.
abstract: Early experiments performed by our group with the phage display technique revealed the potential for using epitope-displaying phages (mimotopes) as a tool for tick antigen discovery. Thus, as a preliminary study, inflammatory reactions induced by phage display tick-borne candidates were investigated by using the cutaneous hypersensitivity test. The profile of selected Rhipicephalus microplus mimotopes was assessed on tick field-exposed cattle and our data indicated a pattern similar to immediate hypersensitivity reaction and not a delayed immune response as expected. However, the wild-type phage inoculation surprisingly induced a strong immediate response on its own. Such reactions indicate that the wild-type phage may have hidden many of the potential reactions raised by the mimotopes. The study of the inflammatory reactions to these phage mimotopes in tick-infested hosts may provide basic information about the immune reaction. Finally, this work is of relevance for when considering research alternatives for finding and characterization of antigens by the phage display technique.
authors.affiliation: Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia-UFU, Campus Umuarama 2E-248, 38400-902 Uberlândia, MG, Brazil. crprudencio@usp.br
authors.forename: Carlos Roberto
authors.initials: CR
authors.lastname: Prudencio
authors.forename: Aline Aparecida
authors.initials: AA
authors.lastname: Rezende Rodrigues
authors.forename: Rone
authors.initials: R
authors.lastname: Cardoso
authors.forename: Guilherme Rocha Lino
authors.initials: GR
authors.lastname: de Souza
authors.forename: Matias Pablo Juan
authors.initials: MP
authors.lastname: Szabó
authors.forename: Luiz Ricardo
authors.initials: LR
authors.lastname: Goulart
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000469:parasitology*; D060467:Disease Resistance; D004198:Disease Susceptibility / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D005260:Female; D007428:Intradermal Tests / Q000662:veterinary*; D048494:Rhipicephalus / Q000276:immunology* / Q000821:virology; D013984:Tick Infestations / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D014612:Vaccines / Q000276:immunology*
chemical_list: D000939:Epitopes; D014612:Vaccines