title: Anti-inflammatory and immunomodulatory efficacy of indigenous probiotic Lactobacillus plantarum Lp91 in colitis mouse model.
abstract: Probiotics can affect the immune homeostasis by altering the gut microbial balance and enhancing the immune system of gut, thus benefits in Inflammatory Bowel Disease, including Crohn's disease and Ulcerative colitis. Relative gene expression of pro, anti-inflammatory cytokines and other molecules in 2,4,6-trinitrobenzene sulfonic acid-induced colitis mouse model against Lactobacillus plantarum Lp91 (L. plantarum Lp91) was investigated by reverse transcription-quantitative PCR (RT-qPCR) using relative expression software tool (REST 2008 V2.0.7). L. plantarum Lp91 evoked significant down regulation of TNF-α and COX2 to 0.026 and 0.077 fold in colitis mouse model. No significant difference in expression of IL-12a cytokine in colitis mouse challenged with L. plantarum Lp91 was also observed. IL-10 was significantly up-regulated to 37.813 and 1.327 fold in colitis and non-colitis mouse challenged with L. plantarum Lp91. While, other anti-inflammatory markers i.e. COX1, IL-4 and IL-6 were significantly up regulated in colitis mouse challenged with L. plantarum Lp91. MUC2 gene was significantly up regulated to 2.216 fold in non-colitis group. L. plantarum Lp91, an indigenous probiotic culture, the main subject of this project exhibited strong immunemodulatory properties under in vivo conditions in mouse colitis model.
authors.affiliation: Department of Food Engineering and Technology, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Duary
authors.forename: Mache Amit
authors.initials: MA
authors.lastname: Bhausaheb
authors.forename: Virender Kumar
authors.initials: VK
authors.lastname: Batish
authors.forename: Sunita
authors.initials: S
authors.lastname: Grover
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003092:Colitis / Q000188:drug therapy* / Q000235:genetics / Q000382:microbiology*; D003106:Colon / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007155:Immunologic Factors / Q000494:pharmacology / Q000627:therapeutic use*; D018836:Inflammation Mediators / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D050296:Microbial Viability / Q000187:drug effects; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism; D019936:Probiotics / Q000494:pharmacology / Q000627:therapeutic use*; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007155:Immunologic Factors; D018836:Inflammation Mediators; D055262:Mucin-2