title: Reliable protein production in a Pseudomonas fluorescens expression system.
abstract: A bottleneck to product development can be reliable expression of active target protein. A wide array of recombinant proteins in development, including an ever growing number of non-natural proteins, is being expressed in a variety of expression systems. A Pseudomonas fluorescens expression platform has been developed specifically for recombinant protein production. The development of an integrated molecular toolbox of expression elements and host strains, along with automation of strain screening is described. Examples of strain screening and scale-up experiments show rapid development of expression strains producing a wide variety of proteins in a soluble active form.
authors.affiliation: Pfenex Inc., 10790 Roselle Street, San Diego, CA 92121, United States. dretallack@pfenex.com
authors.forename: Diane M
authors.initials: DM
authors.lastname: Retallack
authors.forename: Hongfan
authors.initials: H
authors.lastname: Jin
authors.forename: Lawrence
authors.initials: L
authors.lastname: Chew
mesh_terms: D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D000941:Antigens / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004789:Enzyme Activation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility; D014158:Transcription, Genetic; D014612:Vaccines / Q000235:genetics / Q000378:metabolism
chemical_list: D000906:Antibodies; D000941:Antigens; D011994:Recombinant Proteins; D014612:Vaccines