title: Lectin activity of the nucleocytoplasmic EUL protein from Arabidopsis thaliana.
abstract: The Euonymus lectin (EUL) domain was recognized as the structural motif for a novel class of putative carbohydrate binding proteins. Confocal microscopy demonstrated that the lectin from Euonymus europaeus (EEA) as well as the EUL protein from Arabidopsis thaliana (ArathEULS3) are located in the nucleocytoplasmic compartment of the plant cell. ArathEULS3 as well as its EUL domain were successfully expressed in Pichia pastoris and purified. The EUL domain from Arabidopsis interacts with glycan structures containing Lewis Y, Lewis X and lactosamine, indicating that it can be considered a true lectin domain. Despite the high sequence identity between the EUL domains in EEA and ArathEULS3, both domains recognize different carbohydrate structures.
authors.affiliation: Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.
authors.forename: Jonas
authors.initials: J
authors.lastname: Van Hove
authors.forename: Elke
authors.initials: E
authors.lastname: Fouquaert
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.forename: Paul
authors.initials: P
authors.lastname: Proost
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D000595:Amino Acid Sequence; D000606:Amino Sugars / Q000737:chemistry / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000378:metabolism; D016256:Lewis X Antigen / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia; D037121:Plant Lectins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D000606:Amino Sugars; D023181:Antimicrobial Cationic Peptides; D029681:Arabidopsis Proteins; C568478:EULS3 protein, Arabidopsis; C047161:Evonymous europa lectin; D037102:Lectins; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; D037121:Plant Lectins; C032233:lactosamine