title: Plasma proteome changes associated with refractory cytopenia with multilineage dysplasia.
abstract: BACKGROUND
  Refractory cytopenia with multilineage dysplasia (RCMD) is a subgroup of myelodysplastic syndrome (MDS), which belongs to oncohematological diseases, occurring particularly in elderly patients, and represents a heterogeneous group of bone marrow diseases. The goal of this study was to look for plasma proteins that changed quantitatively or qualitatively in RCMD patients.
  
  
  RESULTS
  A total of 46 plasma samples were depleted, proteins were separated by 2D SDS-PAGE (pI 4-7), and proteomes were compared using Progenesis SameSpots statistical software. Proteins were identified by nanoLC-MS/MS. Sixty-one unique, significantly (p < 0.05, ANOVA) different spots were found; proteins in 59 spots were successfully identified and corresponded to 57 different proteins. Protein fragmentation was observed in several proteins: complement C4-A, complement C4-B, inter-alpha-trypsin inhibitor heavy chain H4, and endorepellin.
  
  
  CONCLUSIONS
  This study describes proteins, which change quantitatively or qualitatively in RCMD patients, and represents the first report on significant alterations in C4-A and C4-B complement proteins and ITIH4 fragments in patients with MDS-RCMD.
authors.affiliation: Department of Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic. pavel.majek@uhkt.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Májek
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Reicheltová
authors.forename: Jiří
authors.initials: J
authors.lastname: Suttnar
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Cermák
authors.forename: Jan E
authors.initials: JE
authors.lastname: Dyr