title: Bifunctionalized SBA-15 as a novel micropipette tip sorbent for selective removal and enrichment of biomolecules.
abstract: Selective enrichment or removal of specific proteins prior to analysis can minimize nonspecific interferences as well as information loss, which has been an important issue in current proteomics fields. In this work, two kinds of mesoporous silica SBA-15 (mean pore diameter of 5 nm and 7 nm), bifunctionalized with quaternary ammonium and C18 groups via silylanization reaction, was used to investigate the adsorption behavior for different proteins (bovine serum albumin (BSA), ovalbumin (OVA), hemoglobin (Hb), lysozyme (Lys) and cytochrome c (cyt c)). As possessing anion exchange (quaternary ammonium) groups, the bifunctionalized SBA-15 showed selective adsorption of the negative charged proteins, BSA and OVA. Based on these properties, sequential fractionation based on different SBA-15 materials as micropipette tip sorbents was developed for sample preparation prior to protein analysis. As expected, after the sequential treatment of the sample, the detection signal of the high abundant BSA was significantly decreased and that of the low abundant cyt c was obviously enlarged, which solved the problem that low abundant protein was suppressed by adjacent high abundant protein. The sample preparation technique significantly improved protein identification and this sequential fractionation approach could be potentially applied to extend information on the protein identification for biological samples with a wide dynamic range.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Lan
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Wenfu
authors.initials: W
authors.lastname: Yan
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D006838:Hydrocarbons / Q000737:chemistry*; D016062:Porosity; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006838:Hydrocarbons; D011506:Proteins; D000644:Quaternary Ammonium Compounds; C509969:SBA-15; D012822:Silicon Dioxide