title: Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models.
abstract: Identifying the gene expression alterations that occur in both the tumor and stroma is essential to understanding tumor biology. We have developed a dual-species microarray analysis method that allows the dissection of both tumor and stromal gene expression profiles from xenograft models, based on limited interspecies cross-hybridization on Illumina gene expression beadchips. This methodology allows for simultaneous genome-wide analysis of gene expression profiles of both tumor cells and the associated stromal tissue.
authors.affiliation: Department of Medicine, University of Miami Miller School of Medicine, 1501 NW 10th Ave, BRB 701, Miami, FL 33136, USA. eiorns@med.miami.edu
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Iorns
authors.forename: Jennifer
authors.initials: J
authors.lastname: Clarke
authors.forename: Toby
authors.initials: T
authors.lastname: Ward
authors.forename: Sonja
authors.initials: S
authors.lastname: Dean
authors.forename: Marc
authors.initials: M
authors.lastname: Lippman
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D051379:Mice; D009362:Neoplasm Metastasis / Q000235:genetics; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology*; D059016:Tumor Microenvironment / Q000235:genetics