title: Differential gene expression between inbred Roman high- (RHA-I) and low- (RLA-I) avoidance rats.
abstract: Microarray technology was used to explore differences in brain gene expression under basal conditions in two strains of psychogenetically selected rats which differ in anxiety/stress responses, the inbred Roman High-(RHA-I) and Roman Low-(RLA-I) Avoidance rats. Microarray analysis detected 14 up-regulated and 24 down-regulated genes in RLA-I vs. RHA-I rats functionally related to neurobiological processes. The differentially expressed genes CAMKK2, CRHBP, EPHX2, HOMER3, NDN, PRL and RPL6 were selected for microarray validation using qRT-PCR. EPHX2, CAMKK2 (both up-regulated in RLA-I vs. RHA-I rats) and HOMER3 (down-regulated in RLA-I vs. RHA-I rats) showed a similar tendency and fold-change both in microarray and RT-PCR analyses; PRL (up-regulated in RLA-I vs. RHA-I rats), CRHBP and RPL6 (both down-regulated in RLA-I vs. RHA-I animals) showed a similar tendency but a different order of magnitude of change among experiments; finally, NDN was validated neither in tendency nor in magnitude of change.
authors.affiliation: Department of Psychology, University of Jaén, Campus Las Lagunillas s/n Building C-5, 23071 Jaén, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Sabariego
authors.forename: M José
authors.initials: MJ
authors.lastname: Gómez
authors.forename: Ignacio
authors.initials: I
authors.lastname: Morón
authors.forename: Carmen
authors.initials: C
authors.lastname: Torres
authors.forename: Alberto
authors.initials: A
authors.lastname: Fernández-Teruel
authors.forename: Adolfo
authors.initials: A
authors.lastname: Tobeña
authors.forename: Toni
authors.initials: T
authors.lastname: Cañete
authors.forename: José A
authors.initials: JA
authors.lastname: Martínez-Conejero
authors.forename: José A
authors.initials: JA
authors.lastname: Horcajadas
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Esteban
mesh_terms: D000818:Animals; D001007:Anxiety / Q000235:genetics; D001362:Avoidance Learning; D001921:Brain / Q000378:metabolism*; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D004851:Epoxide Hydrolases / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D000072233:Homer Scaffolding Proteins; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D011388:Prolactin / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics; D013315:Stress, Psychological / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D000072233:Homer Scaffolding Proteins; C000606628:Homer3 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C031249:ribosomal protein L6; C067133:corticotropin releasing factor-binding protein; D011388:Prolactin; D054737:Calcium-Calmodulin-Dependent Protein Kinase Kinase; C517702:Camkk2 protein, rat; D004851:Epoxide Hydrolases; C529022:EPHX2 protein, rat