title: Decrease in CD8+ lymphocyte number and altered cytokine profile in human prostate cancer.
abstract: The tumor microenvironment is comprised of multiple cell types arranged in a three-dimensional structure. Interactions amongst the various cell components play an important role in neoplasia, including the inflammatory reaction that occurs as part of the host response. In this study, the regional lymphocyte subpopulations and cytokine profiles associated with prostate cancer were examined using a quantitative imaging approach and expression microarray analysis. Lymphocytes were measured in four different epithelial phenotypes in prostate cancer specimens: carcinoma; prostatic intraepithelial neoplasia (PIN); benign prostate hyperplasia (BPH); and normal epithelium. The data indicate that CD8 positive, cytotoxic T lymphocytes are significantly decreased in regions adjacent to hyperplasia and carcinoma as compared to normal epithelium and PIN. In contrast the relative number of CD4 positive and CD20 positive lymphocytes did not change markedly. Parallel mRNA expression array analysis of the normal and tumor microenvironments identified a distinct cytokine profile in cancer, with 24 dysregulated genes in tumor epithelium and nine altered in tumor-associated stroma. Overall, these data indicate that the spatial distribution of CD8 positive, cytotoxic T lymphocytes is dysregulated in human prostate glands that contain cancer, and cytokine profiles are altered at the mRNA level.
authors.forename: Gallya
authors.initials: G
authors.lastname: Gannot
authors.forename: Annely M
authors.initials: AM
authors.lastname: Richardson
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodriguez-Canales
authors.forename: Peter A
authors.initials: PA
authors.lastname: Pinto
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Merino
authors.forename: Rodrigo F
authors.initials: RF
authors.lastname: Chuaqui
authors.forename: John W
authors.initials: JW
authors.lastname: Gillespie
authors.forename: Michael R
authors.initials: MR
authors.lastname: Emmert-Buck