title: Rapid high-performance liquid chromatographic method for determination of adefovir in plasma using UV detection: application to pharmacokinetic studies.
abstract: A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of adefovir (CAS 106941-25-7) in human plasma. The separation was achieved on a monolithic silica column (Chromolith Performance RP-18e, 100 x 4.6 mm) using acetonitrile-ammonium dihydrogen phosphate buffer (6:94, v/v), pH 5.2, as the mobile phase at a flow rate of 1.5 ml min(-1). The wavelength was set at 260 nm. The assay enables the measurement of adefovir for therapeutic drug monitoring with a minimum quantification limit of 1 ng ml(-1). The method involves a simple protein precipitation procedure. Analytical recovery was complete. The calibration curve was linear over the concentration range 1-40 ng ml(-1). The coefficients of variation for inter-day and intra-day assay were found to be less than 5%. The method was applied to the determination of adefovir in plasma from 12 subjects dosed with adefovir 2 x 10 mg tablets and pharmacokinetic parameters were evaluated.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. mforoutan@excite.com
authors.forename: Seyed Mohsen
authors.initials: SM
authors.lastname: Foroutan
authors.forename: Afshin
authors.initials: A
authors.lastname: Zarghi
authors.forename: Alireza
authors.initials: A
authors.lastname: Shafaati
authors.forename: Hooman
authors.initials: H
authors.lastname: Movahed
authors.forename: Arash
authors.initials: A
authors.lastname: Khoddam
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D000328:Adult; D000998:Antiviral Agents / Q000097:blood* / Q000493:pharmacokinetics*; D019540:Area Under Curve; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D063065:Organophosphonates / Q000097:blood* / Q000493:pharmacokinetics*; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000998:Antiviral Agents; D007202:Indicators and Reagents; D063065:Organophosphonates; D012996:Solutions; C053001:adefovir; D000225:Adenine