title: The 1,4-naphthoquinone derivative from Pyrola rotundifolia activates AMPK phosphorylation in C2C12 myotubes.
abstract: An aqueous ethanol extract of Pyrola rotundifolia L. induced AMP-activated protein kinase (AMPK) phosphorylation in C2C12 myotubes. The bioassay-guided fractionation of the extract led to the isolation a 2-methyl-7-hydroxymethyl-1,4-naphthoquinone, or a 7'-hydroxy-chimaphilin, which showed concentration-dependent AMPK phosphorylation activity at 2.5-20 μg/ml. At a concentration of 10 μg/ml (50 μM), an approximately four-fold increase in the AMPKα(Thr¹⁷²) phosphorylation level was observed. The stimulatory effect of naphthoquinone on AMPK activity was comparable to that of known compounds found in natural sources that activate the AMPK signaling pathway.
authors.affiliation: Ajinomoto-Genetika Research Institute, 1st Dorozhny Pr. 1-1, Moscow 117545, Russian Federation. leonid_ptitsyn@agri.ru
authors.forename: Leonid R
authors.initials: LR
authors.lastname: Ptitsyn
authors.forename: Kenzo
authors.initials: K
authors.lastname: Nomura
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Sklyar
authors.forename: Anna B
authors.initials: AB
authors.lastname: Ravcheeva
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D020536:Enzyme Activators / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009285:Naphthoquinones / Q000302:isolation & purification / Q000494:pharmacology*; D010766:Phosphorylation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D031828:Pyrola / Q000737:chemistry*
chemical_list: C571582:2-methyl-7-hydroxymethyl-1,4-naphthoquinone; D020536:Enzyme Activators; D009285:Naphthoquinones; D010936:Plant Extracts; D055372:AMP-Activated Protein Kinases; C035342:1,4-naphthoquinone