title: An alternative paper based tissue washing method for mass spectrometry imaging: localized washing and fragile tissue analysis.
abstract: Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging.
authors.affiliation: FOM Institute AMOLF, Science Park 104, 1098 XG, Amsterdam, The Netherlands.
authors.forename: Erika R Amstalden
authors.initials: ER
authors.lastname: van Hove
authors.forename: Donald F
authors.initials: DF
authors.lastname: Smith
authors.forename: Lara
authors.initials: L
authors.lastname: Fornai
authors.forename: Kristine
authors.initials: K
authors.lastname: Glunde
authors.forename: Ron M A
authors.initials: RM
authors.lastname: Heeren
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D005260:Female; D016591:Histocytological Preparation Techniques / Q000379:methods*; D008297:Male; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D009206:Myocardium / Q000737:chemistry; D010209:Paper; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D051381:Rats; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013499:Surface Properties
chemical_list: D010455:Peptides; D011506:Proteins