title: Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging.
abstract: We describe a mass spectrometry method, QuantMode, which improves accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference, simultaneous isolation and fragmentation of impurities, through gas-phase purification. QuantMode analysis of a yeast sample 'contaminated' with interfering human peptides showed substantially improved quantitative accuracy compared to a standard scan, with a small loss of spectral identifications. This technique enables large-scale, multiplexed quantitative proteomics using isobaric tagging.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
authors.forename: Craig D
authors.initials: CD
authors.lastname: Wenger
authors.forename: M Violet
authors.initials: MV
authors.lastname: Lee
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Hebert
authors.forename: Graeme C
authors.initials: GC
authors.lastname: McAlister
authors.forename: Douglas H
authors.initials: DH
authors.lastname: Phanstiel
authors.forename: Michael S
authors.initials: MS
authors.lastname: Westphall
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Coon
mesh_terms: D005740:Gases; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D020543:Proteome; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005740:Gases; D011506:Proteins; D020543:Proteome