title: Purification and identification of antioxidant peptides from egg white protein hydrolysate.
abstract: Egg white proteins were hydrolysed separately using five different proteases to obtain antioxidant peptides. The antioxidant activity of egg white protein hydrolysates was influenced by the time of hydrolysis and the type of enzyme. Of the various hydrolysates produced, papain hydrolysate obtained by 3-h hydrolysis (PEWPH) displayed the highest DPPH radical scavenging activity. PEWPH could also quench the superoxide anion and hydroxyl radicals, effectively inhibit lipid peroxidation and exhibit reducing power. Then, PEWPH was purified sequentially by ultrafiltration, gel filtration, RP-HPLC and two fractions with relatively strong antioxidant activity were subsequently subjected to LC-MS/MS for peptide sequence identification. The sequences of the two antioxidant peptides were identified to be Tyr-Leu-Gly-Ala-Lys (551.54 Da) and Gly-Gly-Leu-Glu-Pro-Ile-Asn-Phe-Gln (974.55 Da), and they were identified for the first time from food-derived protein hydrolysates. Last, the two purified peptides were synthesized and they showed 7.48- and 6.02-fold higher DPPH radical scavenging activity compared with the crude PEWPH, respectively. These results indicate that PEWPH and/or its isolated peptides may be useful ingredients in food and nutraceutical applications.
authors.affiliation: College of Food Science, Northeast Agricultural University, 59 Mucai road, Xiangfang District, Harbin, 150030, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Chi
authors.forename: Ming-Yang
authors.initials: MY
authors.lastname: Zhao
authors.forename: Lei
authors.initials: L
authors.lastname: Lv
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D004527:Egg Proteins / Q000737:chemistry*; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D010206:Papain / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D011492:Protein Hydrolysates; D020539:Sequence Analysis, Protein; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004527:Egg Proteins; D010446:Peptide Fragments; D011492:Protein Hydrolysates; D013481:Superoxides; D010206:Papain