title: Linoleic acid induces an EMT-like process in mammary epithelial cells MCF10A.
abstract: Epidemiological studies and animal models suggest an association between high levels of dietary fat intake and an increased risk of developing breast cancer. Epithelial-mesenchymal-transition (EMT) is a process, by which epithelial cells are transdifferentiated to a mesenchymal state, and it has been implicated in cancer progression, including invasion and metastasis. Linoleic acid (LA) induces proliferation and invasion in breast cancer cells. However, the role of LA on the EMT process in human mammary epithelial cells remains to be studied. In the present study, we demonstrate that LA induces a transient down-regulation of E-cadherin expression, accompanied with an increase of Snail1, Snail2, Twist1, Twist2 and Sip1 expressions. Furthermore, LA induces FAK and NFκB activation, MMP-2 and -9 secretions, migration and invasion. In summary, our findings demonstrate, for the first time, that LA promotes an EMT-like process in MCF10A human mammary epithelial cells.
authors.affiliation: Departamento de Biologia Celular, Cinvestav-IPN, Av. IPN # 2508, San Pedro Zacatenco, Mexico, DF 07360, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Espinosa-Neira
authors.forename: Janini
authors.initials: J
authors.lastname: Mejia-Rangel
authors.forename: Pedro
authors.initials: P
authors.lastname: Cortes-Reynosa
authors.forename: Eduardo Perez
authors.initials: EP
authors.lastname: Salazar
mesh_terms: D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002465:Cell Movement; D054338:Cell Transdifferentiation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology*; D042361:Mammary Glands, Human / Q000166:cytology* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism
chemical_list: D015820:Cadherins; D016328:NF-kappa B; D019787:Linoleic Acid; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9