title: Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow.
abstract: OBJECTIVE
  The aim of this study is to compare the gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow for characterization of dental stem cells.
  
  
  METHODS
  We employed GeneChip analysis to the expression levels of approximately 32,321 kinds of transcripts in 5 samples of bone-marrow-derived mesenchymal stem cells (BMSCs) (n=1), periodontal ligament stem cells (PDLSCs) (n=2), and dental pulp stem cells (DPSCs) (n=2). Each cell was sorted by a FACS Vantage Sorter using immunocytochemical staining of the early mesenchymal stem cell surface marker STRO-1 before the microarray analysis.
  
  
  RESULTS
  We identified 379 up-regulated and 133 down-regulated transcripts in BMSCs, 68 up-regulated and 64 down-regulated transcripts in PDLSCs, and 218 up-regulated and 231 down-regulated transcripts in DPSCs. In addition, anatomical structure development and anatomical structure morphogenesis gene ontology (GO) terms were over-represented in all three different mesenchymal stem cells and GO terms related to blood vessels, and neurons were over-represented only in DPSCs.
  
  
  CONCLUSIONS
  This study demonstrated the genome-wide gene expression patterns of STRO-1(+) mesenchymal stem cells derived from dental tissues and bone marrow. The differences among the expression profiles of BMSCs, PDLSCs, and DPSCs were shown, and 999 candidate genes were found to be definitely up- or down-regulated. In addition, GOstat analyses of regulated gene products provided over-represented GO classes. These data provide a first step for discovering molecules key to the characteristics of dental stem cells.
authors.affiliation: Department of Periodontics, Asan Medical Center, Seoul, Korea.
authors.forename: Su-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Young-Sung
authors.initials: YS
authors.lastname: Kim
authors.forename: Su-Yeon
authors.initials: SY
authors.lastname: Lee
authors.forename: Kyoung-Hwa
authors.initials: KH
authors.lastname: Kim
authors.forename: Yong-Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Kim
authors.forename: Young-Kyoo
authors.initials: YK
authors.lastname: Lee