title: Species-specific myoglobin oxidation.
abstract: The effect of the lipid oxidation product, 4-hydroxy-2-nonenal (HNE), on oxidation of oxymyoglobin (OxyMb) from seven different meat-producing species was investigated. Relative to controls, HNE increased OxyMb oxidation within all species (p < 0.05) at both 25 and 4 °C, pH 5.6. The relative effect of HNE was greater for myoglobins (Mbs) that contained 12 ± 1 histidine (His) residues than for those that contained 9 His residues (p < 0.05); HNE efficacy in all species except chicken and turkey decreased with time. Mono-HNE adducts were detected in all species except chicken and turkey. In general, HNE alkylation increased the Mbs' ability to accelerate lipid oxidation in a microsome model. However, neither an HNE nor a Mb species dependent effect was observed. Results suggested that microsome model system associated lipid oxidation overshadowed HNE and species effects on OxyMb oxidation observed in lipid-free systems.
authors.affiliation: Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269-4040, United States.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yin
authors.forename: Cameron
authors.initials: C
authors.lastname: Faustman
authors.forename: Nantawat
authors.initials: N
authors.lastname: Tatiyaborworntham
authors.forename: Ranjith
authors.initials: R
authors.lastname: Ramanathan
authors.forename: Naveena B
authors.initials: NB
authors.lastname: Maheswarappa
authors.forename: Richard A
authors.initials: RA
authors.lastname: Mancini
authors.forename: Poulson
authors.initials: P
authors.lastname: Joseph
authors.forename: Surendranath P
authors.initials: SP
authors.lastname: Suman
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D010084:Oxidation-Reduction; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014422:Turkeys
chemical_list: D009211:Myoglobin