title: [Comparative analysis of the informativeness of ISSR markers for estimating genetic diversity of horse breeds].
abstract: The informativeness of six different types of ISSR markers was compared in order to evaluate genetic diversity of Mongolian, Buryat and Tuvinian horse breeds (Equus caballus). The dinucleotide-based ISSR primers ((GA)9C, (AG)9C, and (CA)9G) proved to be of little use to examine genetic polymorphism among horses due to the low number of amplified and polymorphic fragments. The polymorphism information content (PIC) for the dinucleotide-based ISSR primers was 2.88 times lower than that for trinucleotide-based ISSR markers. Among the trinucleotide-based ISSR markers, CAC ISSR marker was also found to be noninformative, due to the low number of polymorphic loci identified. The highest values of the polymorphism information content (PIC) were obtained for two types of ISSR markers, based on (GAG)6C (PIC, 3.61) and on (ACC)6G (PIC, 2.50). Thus, the results of the present study showed that GAG and ACC ISSR markers were most informative for evaluation of genetic diversity among horses.
authors.forename: V N
authors.initials: VN
authors.lastname: Voronkova
authors.forename: Tsendsuren
authors.initials: T
authors.lastname: Tsedev
authors.forename: G E
authors.initials: GE
authors.lastname: Sylimova
mesh_terms: D000818:Animals; D001947:Breeding; D018900:Dinucleotide Repeats / Q000235:genetics*; D005819:Genetic Markers; D006736:Horses / Q000235:genetics*; D008986:Mongolia; D011110:Polymorphism, Genetic; D012800:Siberia
chemical_list: D005819:Genetic Markers