title: Molecular characterization of a novel 14-3-3 protein gene (Hb14-3-3c) from Hevea brasiliensis.
abstract: The cDNA encoding a 14-3-3 protein, designated as Hb14-3-3c, was isolated from Hevea brasiliensis. Hb14-3-3c was 1,269 bp long containing a 795 bp open reading frame encoding a putative protein of 264 amino acids, flanked by a 146 bp 5'UTR and a 328 bp 3' UTR. The predicted molecular mass of Hb14-3-3c is 29.67 kDa, with an isoelectric point of 4.52 and the deduced protein showed high similarity to the 14-3-3 protein from other plant species. Expression analysis revealed more significant accumulation of Hb14-3-3c transcripts in latex than in leaves, buds and flowers. The transcription of Hb14-3-3c in latex was induced by jasmonate and ethephon. Overproduction of recombinant Hb14-3-3c protein gave the Escherichia coli cells more tolerance on Co(2+), Cu(2+) and Zn(2+). Through yeast two-hybrid screening, 11 interaction partners of the Hb14-3-3c, which are involved in rubber biosynthesis, stress-related responses, defence etc., were identified in rubber tree latex. Taking these data together, it is proposed that the Hb14-3-3c may participate in regulation of rubber biosynthesis. Thus, the results of this study provide novel insights into the 14-3-3 signaling related to rubber biosynthesis, stress-related responses in rubber tree.
authors.affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China. yangziping302@163.com
authors.forename: Zi-Ping
authors.initials: ZP
authors.lastname: Yang
authors.forename: Hui-Liang
authors.initials: HL
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Guo
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Tian
authors.forename: Shi-Qing
authors.initials: SQ
authors.lastname: Peng
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D028482:Hevea / Q000187:drug effects / Q000235:genetics*; D007840:Latex / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000235:genetics; D009943:Organophosphorus Compounds / Q000494:pharmacology; D054883:Oxylipins / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D020033:Protein Isoforms / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048948:14-3-3 Proteins; D003517:Cyclopentanes; D007840:Latex; D009943:Organophosphorus Compounds; D054883:Oxylipins; D010940:Plant Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C011006:jasmonic acid; C005073:ethephon