title: The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke.
abstract: Although several risk factors for stroke have been identified, one-third remain unexplained. Here we show that infection with Streptococcus mutans expressing collagen-binding protein (CBP) is a potential risk factor for haemorrhagic stroke. Infection with serotype k S. mutans, but not a standard strain, aggravates cerebral haemorrhage in mice. Serotype k S. mutans accumulates in the damaged, but not the contralateral hemisphere, indicating an interaction of bacteria with injured blood vessels. The most important factor for high-virulence is expression of CBP, which is a common property of most serotype k strains. The detection frequency of CBP-expressing S. mutans in haemorrhagic stroke patients is significantly higher than in control subjects. Strains isolated from haemorrhagic stroke patients aggravate haemorrhage in a mouse model, indicating that they are haemorrhagic stroke-associated. Administration of recombinant CBP causes aggravation of haemorrhage. Our data suggest that CBP of S. mutans is directly involved in haemorrhagic stroke.
authors.affiliation: Department of Pediatric Dentistry, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nakano
authors.forename: Kazuya
authors.initials: K
authors.lastname: Hokamura
authors.forename: Naho
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Koichiro
authors.initials: K
authors.lastname: Wada
authors.forename: Chiho
authors.initials: C
authors.lastname: Kudo
authors.forename: Ryota
authors.initials: R
authors.lastname: Nomura
authors.forename: Ayuchi
authors.initials: A
authors.lastname: Kojima
authors.forename: Shuhei
authors.initials: S
authors.lastname: Naka
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Muranaka
authors.forename: Min
authors.initials: M
authors.lastname: Thura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Masuda
authors.forename: Ichiro
authors.initials: I
authors.lastname: Nakagawa
authors.forename: Pietro
authors.initials: P
authors.lastname: Speziale
authors.forename: Nobumitsu
authors.initials: N
authors.lastname: Shimada
authors.forename: Atsuo
authors.initials: A
authors.lastname: Amano
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kamisaki
authors.forename: Tokutaro
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
authors.forename: Takashi
authors.initials: T
authors.lastname: Ooshima
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D051379:Mice; D010974:Platelet Aggregation; D051381:Rats; D011918:Rats, Inbred SHR; D013295:Streptococcus mutans / Q000378:metabolism* / Q000472:pathogenicity; D020521:Stroke / Q000378:metabolism*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D003094:Collagen