title: Effects of desipramine on the antioxidant status in rat tissues at carrageenan-induced paw inflammation.
abstract: The pathogenesis of many diseases and different pathological conditions, including inflammation, is associated with excess production of reactive oxygen species (ROS). The present study aimed to investigate the effects of the antidepressant desipramine (DES) on carrageenan (CG)-induced inflammation, as well as on the endogenous levels of cell enzyme and non-enzyme antioxidants in rat liver and spleen, 4 and 24 h after CG injection. The intra-plantar CG injection into the right hind paw resulted in a time-dependent increase in the paw volume; the maximum of CG-induced edema peak was in 2-4 h. A single DES dose of 20 mg · kg(-1) , administered 30 min before CG, had no effect on paw edema, whereas the higher drug dose used (50 mg · kg(-1) ) suppressed the edematous response to CG. The latter drug dose protected CG-induced decrease of glutathione (non-enzyme antioxidant) in the liver; it did not affect CG-unchanged activities of superoxide dismutase, glutathione peroxidase (enzyme antioxidants) and glucose-6-phosphate dehydrogenase (enzyme, important for the activity of glutathione-conjugated antioxidant enzymes) in both liver and spleen. The drug showed an efficient antioxidant capacity in ROS-generating chemical systems; it was higher than that of fluoxetine (another type of antidepressant). The present results suggest that the good antioxidant activity of DES might contribute to its beneficial effects in liver injuries.
authors.affiliation: Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria. stefany18@abv.bg.
authors.forename: Stefani
authors.initials: S
authors.lastname: Vircheva
authors.forename: Galina
authors.initials: G
authors.lastname: Nenkova
authors.forename: Almira
authors.initials: A
authors.lastname: Georgieva
authors.forename: Albena
authors.initials: A
authors.lastname: Alexandrova
authors.forename: Elina
authors.initials: E
authors.lastname: Tzvetanova
authors.forename: Polina
authors.initials: P
authors.lastname: Mateeva
authors.forename: Rositsa
authors.initials: R
authors.lastname: Zamfirova
authors.forename: Margarita
authors.initials: M
authors.lastname: Kirkova
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism* / Q000627:therapeutic use*; D002351:Carrageenan; D003891:Desipramine / Q000627:therapeutic use*; D005121:Extremities; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
chemical_list: D000928:Antidepressive Agents; D000975:Antioxidants; D017382:Reactive Oxygen Species; D002351:Carrageenan; D003891:Desipramine