title: Isolation of a gastroprotective arabinoxylan from sugarcane bagasse.
abstract: After industrial processing, one-third of sugarcane culms is converted into residual bagasse. The xylan-rich hemicellulose components of the bagasse were extracted with hot aqueous alkali (AX-CRUDE). Approximately 82% of the extracted hemicelluloses was precipitated with ethanol (AX-PET). Both AX-CRUDE and AX-PET contained an arabinoxylan as confirmed by 13C NMR and methylation analysis. Fraction AX-PET was fed to female Wistar rats with ethanol-induced gastric lesions. Oral administrations of 30, 100, and 300 mg/kg reduced the gastric lesion area by over 50%, and replenished ethanol-induced depletion of glutathione. The polysaccharide also increased mucus production by over 70%, indicating its cytoprotective action on experimentally induced gastric ulcers. These findings are significant, since a biologically active compound can be extracted in high yields from an abundant, readily available residue.
authors.affiliation: Embrapa Agroindústria de Alimentos, Av. das Américas, 29501, 23.020-470 Rio de Janeiro, RJ, Brazil. caroline@ctaa.embrapa.br
authors.forename: Caroline
authors.initials: C
authors.lastname: Mellinger-Silva
authors.forename: Fernanda F
authors.initials: FF
authors.lastname: Simas-Tosin
authors.forename: Daniele N
authors.initials: DN
authors.lastname: Schiavini
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Werner
authors.forename: Cristiane H
authors.initials: CH
authors.lastname: Baggio
authors.forename: Isabela T
authors.initials: IT
authors.lastname: Pereira
authors.forename: Luisa M
authors.initials: LM
authors.lastname: da Silva
authors.forename: Philip A J
authors.initials: PA
authors.lastname: Gorin
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D011232:Chemical Precipitation / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D005260:Female; D008745:Methylation / Q000187:drug effects; D009005:Monosaccharides / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D031786:Saccharum / Q000737:chemistry*; D013276:Stomach Ulcer / Q000188:drug therapy*; D013402:Sugar Alcohols / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014990:Xylans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000897:Anti-Ulcer Agents; D009005:Monosaccharides; D013402:Sugar Alcohols; D014990:Xylans; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; C085118:arabinoxylan; D002374:Catalase; D013482:Superoxide Dismutase