title: Oversampling method to extract excitatory and inhibitory conductances from single-trial membrane potential recordings.
abstract: Variations of excitatory and inhibitory conductances determine the membrane potential (V(m)) activity of neurons, as well as their spike responses, and are thus of primary importance. Methods to estimate these conductances require clamping the cell at several different levels of V(m), thus making it impossible to estimate conductances from "single trial" V(m) recordings. We present here a new method that allows extracting estimates of the full time course of excitatory and inhibitory conductances from single-trial V(m) recordings. This method is based on oversampling of the V(m). We test the method numerically using models of increasing complexity. Finally, the method is evaluated using controlled conductance injection in cortical neurons in vitro using the dynamic-clamp technique. This conductance extraction method should be very useful for future in vivo applications.
authors.affiliation: Unité de Neurosciences, Information et Complexité, CNRS, 91198 Gif-sur-Yvette, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Bédard
authors.forename: Sebastien
authors.initials: S
authors.lastname: Béhuret
authors.forename: Charlotte
authors.initials: C
authors.lastname: Deleuze
authors.forename: Thierry
authors.initials: T
authors.lastname: Bal
authors.forename: Alain
authors.initials: A
authors.lastname: Destexhe
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000465:Algorithms; D000818:Animals; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology*; D006801:Humans; D053444:Inhibitory Postsynaptic Potentials / Q000502:physiology*; D008959:Models, Neurological; D009433:Neural Inhibition / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods*