title: Differences in tissue expression of HBV markers in children with HBV-associated glomerulonephritis.
abstract: OBJECTIVE
  Hepatitis B virus-associated glomerulonephritis (HBV-GN) is recognized as one of the major secondary nephropathies in HBV high-risk areas. To determine possible differences in the expression of HBV immune markers in tissues, we retrospectively examined HBV immune markers in the serum, renal tissues, and liver tissues in 132 HBV-GN children.
  
  
  METHODS
  All 132 patients had biopsy-proven HBV-GN including the presence of positive HBV antigens in the kidney. Serum-HBV immune markers were tested by an enzyme-linked immunosorbent assay. Renal and liver biopsies were done in 26 patients. All renal tissues were examined for HBV immune markers by immunofluorescence, and liver tissues were examined by immunohistochemistry.
  
  
  RESULTS
  Among the 132 patients, all showed varying degrees of kidney injury. Serum hepatitis B envelope antigen (HBeAg) was positive in 80 patients and negative in 52 patients. The positivity rate of Hepatitis B core antigen in renal tissue was statistically higher in serum HBeAg (-) than in serum HBeAg (+) patients (96.2% vs. 55.0%). Furthermore, there was no relationship between the presence of hepatitis B surface antigen and HBcAg in liver and renal tissue.
  
  
  CONCLUSIONS
  HBV markers are not consistently present in serum, renal tissues, and liver tissues in children with HBV-GN.
authors.affiliation: Laboratory of Pediatric Nephrology, Institute of Pediatrics, The Second Xiangya Hospital, Central South University and Hunan Province Clinical Center of Pediatric Nephrology, Changsha, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Aiwen
authors.initials: A
authors.lastname: Huang
authors.forename: Pin
authors.initials: P
authors.lastname: Zhou
authors.forename: Xiqiang
authors.initials: X
authors.lastname: Dang
authors.forename: Shuanghong
authors.initials: S
authors.lastname: Mo
authors.forename: Zhuwen
authors.initials: Z
authors.lastname: Yi
authors.forename: Qingnan
authors.initials: Q
authors.lastname: He
mesh_terms: D000293:Adolescent; D000367:Age Factors; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D001707:Biopsy, Needle; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005921:Glomerulonephritis / Q000209:etiology / Q000473:pathology / Q000821:virology*; D006512:Hepatitis B Core Antigens / Q000032:analysis / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D012189:Retrospective Studies; D012307:Risk Factors; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens