title: Interference by the activated sludge matrix on the analysis of soluble microbial products in wastewater.
abstract: The objective of this study was to demonstrate the effects of complex matrix effects caused by chemical materials on the analysis of key soluble microbial products (SMP) including proteins, humics, carbohydrates, and polysaccharides in activated sludge samples. Emphasis was placed on comparison of the commonly used standard curve technique with standard addition (SA), a technique that differs in that the analytical responses are measured for sample solutions spiked with known quantities of analytes. The results showed that using SA provided a great improvement in compensating for SMP recovery and thus improving measurement accuracy by correcting for matrix effects. Analyte recovery was found to be highly dependent on sample dilution, and changed due to extraction techniques, storage conditions and sample composition. Storage of sample extracts by freezing changed SMP concentrations dramatically, as did storage at 4°C for as little as 1d.
authors.affiliation: University of Guelph, School of Engineering, Guelph, Ontario, Canada. cpotvin@uoguelph.ca
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Potvin
authors.forename: Hongde
authors.initials: H
authors.lastname: Zhou
mesh_terms: D001419:Bacteria / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D003080:Cold Temperature; D005615:Freezing; D006812:Humic Substances / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012722:Sewage / Q000032:analysis* / Q000382:microbiology*; D014865:Waste Disposal, Fluid
chemical_list: D002241:Carbohydrates; D006812:Humic Substances; D011134:Polysaccharides; D011506:Proteins; D012722:Sewage