title: Methyl jasmonate elicitation enhances glycyrrhizin production in Glycyrrhiza inflata hairy roots cultures.
abstract: Hairy roots were induced by infecting stems and leaves of Glycyrrhiza inflata with Agrobacterium rhizogenes ATCC 15834. The optimization of growth and glycyrrhizin accumulation of G. inflata hairy roots was studied. Sucrose (6%, w/v) was optimal for growth and glycyrrhizin accumulation in G. inflata hairy roots. Effects of elicitors like chitosan, methyl jasmonate, and yeast extract on glycyrrhizin production were studied. Methyl jasmonate (100 microM) was most efficient in enhancing glycyrrhizin production up to almost 109 microg/g dry weight on day 5 of elicitation. These results indicate that application of elicitors can enhance the capacity of G. inflata hairy roots to produce glycyrrhizin.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors.forename: Winida
authors.initials: W
authors.lastname: Wongwicha
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D000085:Acetates / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism*; D006035:Glycyrrhiza / Q000378:metabolism*; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D019695:Glycyrrhizic Acid; C072239:methyl jasmonate