title: [Significance of the expression of lymphocytes and cytokines infiltrating in HCC].
abstract: OBJECTIVE
  To reveal the classification of lymphocytes and expression of cytokines infiltrating in HCC,  and investigate the significance of changes of immune microenvironment in HCC.
  
  
  METHODS
  76 tumor and non-tumor tissues of HCC were collected to detect the amount of immune cells in the tissues by FCM and immunohistochemistry (IHC). Th1/Th2 cytokines in tissues were detected by Cytometric bead array (CBA),  and TGF-1,  VEGF detected by ELISA.
  
  
  RESULTS
  There were more CD3(+); T cells,  CD4(+); Th cells,  Treg cells,  and CD45RO(+); memory T cells in tumors than in non-tumor tissues. On the contrary there were less CD8(+); CTLs in tumors. There was negative correlation between Treg and Th or CTL cells. CD69,  which is the early activating factor,  expressed less on CD3(+); T cells and NK cells in tumors than non-tumor tissues. Whereas HLA-DR,  which is the late activating factor,  expressed more on CD3(+); T cells in tumors than non-tumor tissues. More IL-10,  TGF-1 and VEGF were secreted in tumors than non-tumor tissues.
  
  
  CONCLUSIONS
  Along with the decrease of effective immune cells and increase of suppressor immune cells and cytokines,  the lymphocytes infiltrating in the tumor were immune incompitence,  which contributed to the tumorigenesis.
authors.affiliation: Fujian Key Laboratory of Translational Cancer Medicine, Fuzhou, China. yunbin_ye@yahoo.com
authors.forename: Yun-bin
authors.initials: YB
authors.lastname: Ye
authors.forename: Feng
authors.initials: F
authors.lastname: Peng
authors.forename: Jie-yu
authors.initials: JY
authors.lastname: Li
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Lin
authors.forename: Xiong-wei
authors.initials: XW
authors.lastname: Zheng
authors.forename: Dong
authors.initials: D
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Qiao-lian
authors.initials: QL
authors.lastname: Li
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Cai
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D055815:Young Adult
chemical_list: D016207:Cytokines