title: Combined in vivo multiphoton and CARS imaging of healthy and disease-affected human skin.
abstract: We present combined epi-coherent anti-Stokes Raman scattering (CARS) and multiphoton imaging with both chemical discrimination and subcellular resolution on human skin in vivo. The combination of both image modalities enables label-free imaging of the autofluorescence of endogenous fluorophores by two-photon excited fluorescence, as well as imaging of the distribution of intercellular lipids, topically applied substances and water by CARS. As an example for medical imaging, we investigated healthy and psoriasis-affected human skin with both image modalities in vivo and found indications for different lipid distributions on the cellular level.
authors.affiliation: JenLab GmbH, Campus D1.2, 66123 Saarbrücken, and Schillerstr 1, 07745, Jena, Germany. breunig@jenlab.de
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Breunig
authors.forename: Rainer
authors.initials: R
authors.lastname: Bückle
authors.forename: Marcel
authors.initials: M
authors.lastname: Kellner-Höfer
authors.forename: Martin
authors.initials: M
authors.lastname: Weinigel
authors.forename: Jürgen
authors.initials: J
authors.lastname: Lademann
authors.forename: Wolfram
authors.initials: W
authors.lastname: Sterry
authors.forename: Karsten
authors.initials: K
authors.lastname: König
mesh_terms: D005542:Forearm / Q000033:anatomy & histology / Q000473:pathology; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008297:Male; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011565:Psoriasis / Q000473:pathology; D012867:Skin / Q000033:anatomy & histology* / Q000737:chemistry / Q000166:cytology; D012871:Skin Diseases / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014867:Water / Q000737:chemistry
chemical_list: D008055:Lipids; D014867:Water