title: Isolation and characterization of 16 novel  microsatellite loci in two inbred strains of the Chinese hamster (Cricetulus griseus).
abstract: The  Shanyi inbred A and E strains of the Chinese hamster are widely used in  biomedical research, but detailed genetic characterization has been lacking. We  developed microsatellite markers that could be used for genetic diversity  analysis and linkage map construction. We isolated and characterized 16 novel  microsatellite loci from a microsatellite-enriched genomic DNA library. These  loci were genotyped in 48 animals from the two strains, and the polymorphic  information content was determined. In the Shanyi A and E populations, 14 and  15 loci were found to be polymorphic, respectively, with polymorphic information  content ranging from 0.1393 to 0.8082 and from 0.1109 to 0.7397, respectively.  A total of 115 alleles were found for the 16 microsatellite loci in the two  populations; the mean observed heterozygosity (H(O)) was  0.5191 and 0.4333 for the A and E populations, respectively, indicating marked  genetic variation within the two populations. Correspondingly, the F(ST) values  ranged from 0.002 to 0.9253, with an overall mean of 0.1935, indicating  significant genetic difference between the two strains. The population  differentiation levels were substantiated by Nei's genetic distance and full  Bayesian analyses computed with STRUCTURE. Despite the genetic diversity and  differentiation within and between the two inbred populations, the 48  individuals were correctly allocated into their original populations with high statistical  confidence based on these 16 microsatellite loci. These novel microsatellite  loci should be useful genetic markers for these two Chinese hamster inbred  strains.
authors.affiliation: Laboratory Animal Center, Shanxi Medical University, Taiyuan, China.
authors.forename: G-H
authors.initials: GH
authors.lastname: Song
authors.forename: J-N
authors.initials: JN
authors.lastname: Geng
authors.forename: R-Y
authors.initials: RY
authors.lastname: Jia
authors.forename: W-B
authors.initials: WB
authors.lastname: Yue
authors.forename: T-F
authors.initials: TF
authors.lastname: Liu
authors.forename: S-N
authors.initials: SN
authors.lastname: Hu
mesh_terms: D000483:Alleles; D000818:Animals; D006224:Cricetinae / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D016678:Genome; D005838:Genotype; D006579:Heterozygote; D007178:Inbreeding; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers; D004247:DNA