title: Predicting peptide binding affinities to MHC molecules using a modified semi-empirical scoring function.
abstract: The Major Histocompatibility Complex (MHC) plays an important role in the human immune system. The MHC is involved in the antigen presentation system assisting T cells to identify foreign or pathogenic proteins. However, an MHC molecule binding a self-peptide may incorrectly trigger an immune response and cause an autoimmune disease, such as multiple sclerosis. Understanding the molecular mechanism of this process will greatly assist in determining the aetiology of various diseases and in the design of effective drugs. In the present study, we have used the Fresno semi-empirical scoring function and modify the approach to the prediction of peptide-MHC binding by using open-source and public domain software. We apply the method to HLA class II alleles DR15, DR1, and DR4, and the HLA class I allele HLA A2. Our analysis shows that using a large set of binding data and multiple crystal structures improves the predictive capability of the method. The performance of the method is also shown to be correlated to the structural similarity of the crystal structures used. We have exposed some of the obstacles faced by structure-based prediction methods and proposed possible solutions to those obstacles. It is envisaged that these obstacles need to be addressed before the performance of structure-based methods can be on par with the sequence-based methods.
authors.affiliation: Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia.
authors.forename: Webber W P
authors.initials: WW
authors.lastname: Liao
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Arthur
mesh_terms: D000465:Algorithms; D000483:Alleles; D018360:Crystallography, X-Ray / Q000379:methods; D015789:HLA-A2 Antigen / Q000378:metabolism; D059810:HLA-DR Serological Subtypes / Q000378:metabolism; D015798:HLA-DR1 Antigen / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D007107:Immune System; D008285:Major Histocompatibility Complex; D009103:Multiple Sclerosis / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D012016:Reference Values; D012044:Regression Analysis
chemical_list: D015789:HLA-A2 Antigen; D059810:HLA-DR Serological Subtypes; D015798:HLA-DR1 Antigen; C106559:HLA-DR15 antigen; D000949:Histocompatibility Antigens Class II; D010455:Peptides