title: Infrared spectroscopic imaging of renal tumor tissue.
abstract: Fourier transform infrared (FTIR) spectroscopic imaging has been used to probe the biochemical composition of human renal tumor tissue and adjacent normal tissue. Freshly resected renal tumor tissue from surgery was prepared as a thin cryosection and examined by FTIR spectroscopic imaging. Tissue types could be discriminated by utilizing a combination of fuzzy k-means cluster analysis and a supervised classification algorithm based on a linear discriminant analysis. The spectral classification is compared and contrasted with the histological stained image. It is further shown that renal tumor cells have spread in adjacent normal tissue. This study demonstrates that FTIR spectroscopic imaging can potentially serve as a fast and objective approach for discrimination of renal tumor tissue from normal tissue and even in the detection of tumor infiltration in adjacent tissue.
authors.affiliation: Vilnius University, Faculty of Physics, Saulėtekio av. 9, LT-10222 Vilnius, Lithuania. valdas.sablinskas@ff.vu.lt
authors.forename: Valdas
authors.initials: V
authors.lastname: Sablinskas
authors.forename: Vidita
authors.initials: V
authors.lastname: Urboniene
authors.forename: Justinas
authors.initials: J
authors.lastname: Ceponkus
authors.forename: Arvydas
authors.initials: A
authors.lastname: Laurinavicius
authors.forename: Darius
authors.initials: D
authors.lastname: Dasevicius
authors.forename: Feliksas
authors.initials: F
authors.lastname: Jankevicius
authors.forename: Vaiva
authors.initials: V
authors.lastname: Hendrixson
authors.forename: Edmund
authors.initials: E
authors.lastname: Koch
authors.forename: Gerald
authors.initials: G
authors.lastname: Steiner
mesh_terms: D000465:Algorithms; D016000:Cluster Analysis; D016002:Discriminant Analysis; D017143:Fuzzy Logic; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000145:classification; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*