title: Salvianolic acid A protects against vascular endothelial dysfunction in high-fat diet fed and streptozotocin-induced diabetic rats.
abstract: Salvianolic acid A (SalA) is one of the main active ingredients of Salvia miltiorrhizae. The objective of this study was to evaluate the effect of SalA on the diabetic vascular endothelial dysfunction (VED). The rats were given a high-fat and high-sucrose diet for 1 month followed by intraperitoneal injection of streptozotocin (30 mg/kg). The diabetic rats were treated with SalA (1 mg/kg, 90% purity) orally for 10 weeks after modeling, and were given a high-fat diet. Contractile and relaxant responses of aorta rings as well as the serum indications were measured. Our results indicated that SalA treatment decreased the level of serum Von Willebrand factor and ameliorated acetylcholine-induced relaxation and KCl-induced contraction in aorta rings of the diabetic rats. SalA treatment also reduced the serum malondialdehyde, the content of aortic advanced glycation end products (AGEs), and the nitric oxide synthase (NOS) activity as well as the expression of endothelial NOS protein in the rat aorta. Exposure of EA.hy926 cells to AGEs decreased the cell viability and changed the cell morphology, whereas SalA had protective effect on AGEs-induced cellular vitality. Our data suggested that SalA could protect against vascular VED in diabetes, which might attribute to its suppressive effect on oxidative stress and AGEs-induced endothelial dysfunction.
authors.affiliation: Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Yang
authors.forename: Gui-Fen
authors.initials: GF
authors.lastname: Qiang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zhu
authors.forename: Shou-Bao
authors.initials: SB
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Sun
authors.forename: Hai-Guang
authors.initials: HG
authors.lastname: Yang
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Du
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002109:Caffeic Acids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000378:metabolism*; D004032:Diet; D059305:Diet, High-Fat / Q000009:adverse effects; D004730:Endothelium, Vascular / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000378:metabolism; D007773:Lactates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D015394:Molecular Structure; D052250:Nitric Oxide Synthase Type III / Q000032:analysis / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D027561:Salvia miltiorrhiza / Q000737:chemistry; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D002109:Caffeic Acids; D017127:Glycation End Products, Advanced; D007773:Lactates; D014280:Triglycerides; C066201:salvianolic acid A; D052250:Nitric Oxide Synthase Type III