title: Biological performance of hydroxyapatite-biopolymer foams: in vitro cell response.
abstract: Uncoated and biopolymer-coated nanocrystalline hydroxyapatite (HA) macroporous foams are presented as promising candidates as scaffolds for bone tissue regeneration. To this end, foam degradability, the cytotoxic effects on osteoblast-like cells of foam degradation by-products and biocompatibility with osteoblast-like cells were assayed on the three-dimensional (3-D) foam surface. The results show that the 3-D interconnected architectural design of these HA foams allows excellent osteoblast internalization, proliferation and differentiation, exhibiting adequate colonization over the entire scaffold surface with an appropriate degradation rate without any cytotoxic effects.
authors.affiliation: Departamento de Química Inorgánica y Bioinorgánica, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Cicuéndez
authors.forename: Isabel
authors.initials: I
authors.lastname: Izquierdo-Barba
authors.forename: Sandra
authors.initials: S
authors.lastname: Sánchez-Salcedo
authors.forename: Mercedes
authors.initials: M
authors.lastname: Vila
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001704:Biopolymers / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D016062:Porosity / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase