title: Persistence of TEM-52/TEM-92 and SHV-12 extended-spectrum β-lactamases in clinical isolates of Enterobacteriaceae in Italy.
abstract: Extended-spectrum β-lactamases (ESBLs) belonging to the TEM and SHV families were investigated in 583 ESBL-producing Enterobacteriaceae collected at the clinical microbiology laboratories of 11 teaching Italian hospitals. By molecular analysis TEM-type and SHV-type ESBLs were confirmed on 154 and 74 isolates, respectively. High variability was found among TEM-types β-lactamases with the following variants: TEM-5, TEM-6, TEM-12, TEM-15, TEM-24, TEM-26, TEM-29, TEM-52, TEM-92, TEM-134, and TEM-149. Among SHV variants, SHV-2a, SHV-5, SHV-12, and SHV-28 have been detected. The most widespread variants are TEM-52/92 and SHV-12.
authors.affiliation: Department of Sciences and Biomedical Technologies, University of L'Aquila, L'Aquila, Italy.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Perilli
authors.forename: Bernardetta
authors.initials: B
authors.lastname: Segatore
authors.forename: Claudia
authors.initials: C
authors.lastname: Mugnaioli
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Celenza
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
authors.forename: Francesco
authors.initials: F
authors.lastname: Luzzaro
authors.forename: Beatrice
authors.initials: B
authors.lastname: Pini
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicosante
mesh_terms: D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology* / Q000382:microbiology; D006784:Hospitals, Teaching / Q000706:statistics & numerical data; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D047090:beta-Lactams; C445691:TEM-52 beta-lactamase; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases