title: Experimental methods for scanning unnatural amino acid mutagenesis.
abstract: Site-specific incorporation of unnatural amino acids into proteins in vivo relies on the genetic reassignment of nonsense or quadruplet codons. Here, we describe a general procedure for the random introduction of these codons into open reading frames resulting in protein libraries that are scanned with unnatural amino acid residues. These libraries can enable large-scale mutagenesis experiments aimed at understanding and improving protein function.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: T Ashton
authors.initials: TA
authors.lastname: Cropp
mesh_terms: D000596:Amino Acids / Q000235:genetics*; D001483:Base Sequence; D004247:DNA; D004251:DNA Transposable Elements; D016296:Mutagenesis; D016366:Open Reading Frames; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004251:DNA Transposable Elements; D011506:Proteins; D004247:DNA