title: Differences in protein binding and cytokine release from monocytes on commercially sourced tissue culture polystyrene.
abstract: Tissue culture polystyrene (TCPS) is a ubiquitous substrate used by many researchers in the biomedical and biological sciences. Different parameters involved in the production of TCPS, including the treatment time and the use of reactive gases and chemical agents, can have a significant influence on the ultimate surface properties achieved. The assumption that they will all yield a consistent and controlled product has not proven to be true. To provide a better insight into the bioactivity differences in TCPS supplied by different manufacturers, TCPS from three different companies (Sarstedt, Wisent Corp., and Becton Dickinson (BD)) were analyzed for their surface properties, protein adsorption characteristics, and interactions with human monocytes. Marked differences were observed in terms of surface wettability and surface chemistry. Furthermore, Wisent TCPS adsorbed more than twice the amount of serum proteins compared with BD and Sarstedt TCPS. Sarstedt showed significantly more cell retention (more DNA) compared with both BD and Wisent TCPS brands over a 7 day culture period. Cytokine release from monocytes adherent on the three different TCPS also differed significantly, suggesting that the differences in the surface properties were sufficient to differentially mediate monocyte activation. These results have important implications for TCPS research use, in terms of appreciating the interpretation of the data when TCPS is used as a control substrate as well as when it is used where a pre-conditioned state would influence the outcome of the study.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Kyle G
authors.initials: KG
authors.lastname: Battiston
authors.forename: Joanne E
authors.initials: JE
authors.lastname: McBane
authors.forename: Rosalind S
authors.initials: RS
authors.lastname: Labow
authors.forename: J
authors.initials: J
authors.lastname: Paul Santerre
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D056951:Photoelectron Spectroscopy; D011137:Polystyrenes / Q000737:chemistry*; D011485:Protein Binding; D013499:Surface Properties; D017155:Wettability
chemical_list: D001798:Blood Proteins; D016207:Cytokines; D011137:Polystyrenes