title: Effects of capsaicin on nitric oxide synthase isoforms in prepubertal rat ovary.
abstract: Nitric oxide (NO) has emerged as an important intra-ovarian regulatory factor. We investigated effects of low dose capsaicin (CAP) treatment on the different NOS isoforms in prepubertal rat ovaries. Fifteen 21-day-old female Sprague-Dawley rats were divided randomly into three groups. The first group received no treatment, the second group received 0.5 mg/kg/day CAP dissolved in the vehicle, and the third group was treated with the vehicle only. The animals were euthanized by ether inhalation after 15 days and their ovaries were excised. Ovaries were fixed in 10% neutral buffered formalin and embedded in paraffin. Sections were processed for standard immunohistochemistry using the labeled streptavidin-biotin technique for expression of nNOS, eNOS and iNOS. We demonstrated that CAP induced expression of NOS isotypes including eNOS, iNOS and nNOS in prepubertal rat ovaries. CAP may lead to release of NO either directly from nerves or indirectly by evoking release from other cells via the action of neuropeptides that are released from afferent terminals and are involved in regulating female reproductive function.
authors.affiliation: Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Uludag, Bursa, Turkey. bzik@uludag.edu.tr
authors.forename: B
authors.initials: B
authors.lastname: Zik
authors.forename: K
authors.initials: K
authors.lastname: Altunbas
authors.forename: S
authors.initials: S
authors.lastname: Tutuncu
authors.forename: O
authors.initials: O
authors.lastname: Ozden
authors.forename: C G
authors.initials: CG
authors.lastname: Ozguden Akkoc
authors.forename: S
authors.initials: S
authors.lastname: Peker
authors.forename: A
authors.initials: A
authors.lastname: Sevimli
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D009411:Nerve Endings / Q000187:drug effects; D009475:Neurons, Afferent / Q000187:drug effects; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010053:Ovary / Q000033:anatomy & histology / Q000187:drug effects* / Q000201:enzymology* / Q000294:innervation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496322:Nos1 protein, rat; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D002211:Capsaicin