title: Achieving broad molecular insights into dynamic protein interactions by integrated structural-kinetic approaches.
abstract: A network of dynamic protein interactions with their protein partners, substrates, and ligands is known to be crucial for biological function. Revealing molecular and structural-based mechanisms at atomic resolution and in real-time is fundamental for achieving a basic understanding of cellular processes. These technically challenging goals may be achieved by combining time-resolved spectroscopic and structural-kinetic tools, thus providing broad insights into specific molecular events over a wide range of timescales. Here we review representative studies utilizing such an integrated real-time structural approach designed to reveal molecular mechanisms underlying protein interactions at atomic resolution.
authors.affiliation: Departments of Structural Biology and Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Moran
authors.initials: M
authors.lastname: Grossman
authors.forename: Netta
authors.initials: N
authors.lastname: Sela-Passwell
authors.forename: Irit
authors.initials: I
authors.lastname: Sagi
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000378:metabolism; D008667:Metalloproteins / Q000378:metabolism*; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D011487:Protein Conformation; D017510:Protein Folding; D025941:Protein Interaction Mapping / Q000379:methods*; D012996:Solutions / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods*; D013329:Structure-Activity Relationship
chemical_list: D008565:Membrane Proteins; D008667:Metalloproteins; D015335:Molecular Probes; D012996:Solutions