title: Tumor endothelial marker 8 expression in triple-negative breast cancer.
abstract: OBJECTIVE
  Tumor endothelial marker 8 (TEM8) is a tumor endothelial-associated antigen that is having an increasingly recognized role in tumor biology. The expression of TEM8 in triple-negative breast cancer (TNBC) has not yet been characterized.
  
  
  METHODS
  We hypothesize that TEM8 is overexpressed in TNBC and in metastatic TNBC in lymph nodes (LN) compared to normal breast tissue and normal lymphatic tissue, respectively. We studied expression of TEM8 in cases of primary (n=17) and metastatic (n=2) TNBC using immunohistochemical analyses.
  
  
  RESULTS
  All cases demonstrated increased expression of TEM8 in tumor tissue compared to non-cancerous breast tissue. TEM8 was expressed at a higher level in the stroma adjacent to the TNBC in all cases, with focal immunoreactive areas within the tumor. TEM8 was not expressed in normal lymphoid tissue, but showed expression at sites of LN metastases.
  
  
  CONCLUSIONS
  TEM8 would appear to represent a new biologic target for designing novel diagnostic or therapeutic approaches for TNBC.
authors.affiliation: Department of Surgery, University of Florida, P.O. Box 100109, 1600 S.W. Archer Rd., Room 6164, Gainesville, Florida 32610, U.S.A.
authors.forename: Luke G
authors.initials: LG
authors.lastname: Gutwein
authors.forename: Samer Z
authors.initials: SZ
authors.lastname: Al-Quran
authors.forename: Stephen
authors.initials: S
authors.lastname: Fernando
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Fletcher
authors.forename: Edward M
authors.initials: EM
authors.lastname: Copeland
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Grobmyer
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000378:metabolism*
chemical_list: C473287:ANTXR1 protein, human; D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface