title: Manganese sulfide formation via concomitant microbial manganese oxide and thiosulfate reduction.
abstract: The dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1 produced γ-MnS (rambergite) nanoparticles during the concurrent reduction of MnO₂ and thiosulfate coupled to H₂ oxidation. To investigate effect of direct microbial reduction of MnO₂ on MnS formation, two MR-1 mutants defective in outer membrane c-type cytochromes (ΔmtrC/ΔomcA and ΔmtrC/ΔomcA/ΔmtrF) were also used and it was determined that direct reduction of MnO₂ was dominant relative to chemical reduction by biogenic sulfide generated from thiosulfate reduction. Although bicarbonate was excluded from the medium, incubations of strain MR-1 with lactate as the electron donor produced MnCO₃ (rhodochrosite) as well as MnS in nearly equivalent amounts as estimated by micro X-ray diffraction (micro-XRD) analysis. It was concluded that carbonate released from lactate metabolism promoted MnCO₃ formation and that Mn(II) mineralogy was strongly affected by carbonate ions even in the presence of abundant sulfide and weakly alkaline conditions expected to favour the precipitation of MnS. Formation of MnS, as determined by a combination of micro-XRD, transmission electron microscopy, energy dispersive X-ray spectroscopy, and selected area electron diffraction analyses was consistent with equilibrium speciation modelling predictions. Biogenic manganese sulfide may be a manganese sink in the Mn biogeochemical cycle in select environments such as deep anoxic marine basins within the Baltic Sea.
authors.affiliation: Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Ji-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: David W
authors.initials: DW
authors.lastname: Kennedy
authors.forename: Alice
authors.initials: A
authors.lastname: Dohnalkova
authors.forename: Dean A
authors.initials: DA
authors.lastname: Moore
authors.forename: Ponnusamy
authors.initials: P
authors.lastname: Nachimuthu
authors.forename: Samantha B
authors.initials: SB
authors.lastname: Reed
authors.forename: James K
authors.initials: JK
authors.lastname: Fredrickson
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002254:Carbonates / Q000378:metabolism; D003574:Cytochrome c Group / Q000235:genetics; D007773:Lactates / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D017895:Manganese Compounds / Q000378:metabolism*; D009154:Mutation; D010084:Oxidation-Reduction; D010087:Oxides / Q000378:metabolism*; D020592:Shewanella / Q000235:genetics / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002254:Carbonates; D003574:Cytochrome c Group; D007773:Lactates; D017895:Manganese Compounds; D010087:Oxides; D013440:Sulfides; D013885:Thiosulfates; D008345:Manganese; C027424:manganese oxide; C041368:manganese sulfide; C045327:manganese carbonate