title: The gain in brain: novel imaging techniques and multiplexed proteomic imaging of brain tissue ultrastructure.
abstract: The rapid accumulation of neuroproteomics data in recent years has prompted the emergence of novel antibody-based imaging methods that aim to understand the anatomical and functional context of the multitude of identified proteins. The pioneering field of ultrastructural multiplexed proteomic imaging now includes a number of high resolution methods, such as array tomography, stimulated emission depletion microscopy, stochastic optical reconstruction microscopy and automated transmission electron microscopy, which allow a detailed molecular characterization of individual synapses and subsynaptic structures within brain tissues for the first time. While all of these methods still face considerable limitations, a combined complementary approach building on the respective strengths of each method is possible and will enable fascinating research into the proteomic diversity of the nervous system.
authors.affiliation: Stanford University School of Medicine, Department of Molecular and Cellular Physiology, Stanford, CA 94305, USA. kmicheva@stanford.edu
authors.forename: Kristina D
authors.initials: KD
authors.lastname: Micheva
authors.forename: Marcel P
authors.initials: MP
authors.lastname: Bruchez
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D009488:Neurosciences / Q000379:methods*; D040901:Proteomics / Q000379:methods*