title: Carboxymethylation of polysaccharides from Auricularia auricula and their antioxidant activities in vitro.
abstract: The chemical carboxymethylated polysaccharide (CMAAP), which is derived from the poorly water-soluble, crude polysaccharide from Auricularia auricula (AAP), was prepared. The degree of substitution of CMAAP was 0.857, and its solubility in water reached 0.6 mg/mL, much higher than that of AAP (0.1 mg/mL). One fraction, CMAAP22, was purified. Chemical analysis indicated that CMAAP22 was composed of mannose and glucose in a ratio of 1.06:1 with a molecular weight of 3.4 × 10(6)Da. IR spectra and (13)C NMR spectra indicated that the C2, C4 and C6 positions were partially substituted by -CH(2)COOH. AFM revealed that CMAAP22 was spherical particle structure with a diameter of 80-300 nm. Moreover, the antioxidant activity of CMAAP22 was improved, nearly twice as much as that of the AAP, especially in the scavenging of DPPH and ABTS+.
authors.affiliation: School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.
authors.forename: Liuqing
authors.initials: L
authors.lastname: Yang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ye
authors.initials: Y
authors.lastname: Zou
authors.forename: Guanghua
authors.initials: G
authors.lastname: Mao
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000378:metabolism; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D005609:Free Radicals / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D008745:Methylation; D018625:Microscopy, Atomic Force; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D010316:Particle Size; D010851:Picrates / Q000037:antagonists & inhibitors / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology / Q000648:ultrastructure; D012995:Solubility; D012996:Solutions; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D014867:Water
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005609:Free Radicals; D010851:Picrates; D011134:Polysaccharides; D012996:Solutions; D014867:Water; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose; D008358:Mannose