title: Uptake studies for evaluating activity of efflux transporters in a cell line representative of the blood-brain barrier.
abstract: In this unit, protocols are presented for performing uptake studies with bovine brain microvessel endothelial cells (BBMECs) using either radiolabeled compounds, or rhodamine 123 as a marker for a P-glycoprotein substrate. The protocols can easily be modified for the investigation of substrate uptake in other cell lines.
authors.affiliation: University of Kansas, Lawrence, KS, USA.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Silverstein
authors.forename: D Nedra
authors.initials: DN
authors.lastname: Karunaratne
authors.forename: Kenneth L
authors.initials: KL
authors.lastname: Audus
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000818:Animals; D001812:Blood-Brain Barrier; D002417:Cattle; D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D017239:Paclitaxel / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017239:Paclitaxel