title: Er,CR:YSGG lasers induce fewer dysplastic-like epithelial artefacts than CO2 lasers: an in vivo experimental study on oral mucosa.
abstract: Our aim was to assess wounds made by lasers (CO(2) and Er,Cr:YSGG) for their epithelial architectural changes and width of damage. We allocated 60 Sprague-Dawley(®) rats into groups: glossectomy by CO(2) laser at 3 different wattages (n=10 in each); glossectomy by Er,Cr:YSGG laser at two different emissions (n=10 in each), and a control group (n=10). Histological examination assessed both prevalence and site of thermal artefacts for each group. Both lasers (CO(2) and Er,Cr:YSGG) caused the same type of cytological artefacts. The 3W Er,Cr:YSGG laser produced the fewest cytological artefacts/specimen, and was significantly different from the other experimental groups: 3W CO(2) laser (95% CI=0.8 to 1.0); the 6W CO(2) laser (95% CI=0.1 to 2.0) and the 10W CO(2) laser (95% CI=1.1 to 3.0). CO(2) lasers (3-10W) generate epithelial damage that can simulate dysplastic changes with cytological atypia that affects mainly the basal and suprabasal layers. Irradiation with Er,CR:YSGG laser (2-4W) produces significantly fewer cellular artefacts and less epithelial damage, which may be potentially useful for biopsy of oral mucosa.
authors.affiliation: Stomatology Department, School of Medicine and Dentistry, University of Santiago de Compostela, Entrerríos s/n, 15782 Santiago de Compostela, Spain. antonio.gonzalez@usc.es
authors.forename: A
authors.initials: A
authors.lastname: González-Mosquera
authors.forename: J
authors.initials: J
authors.lastname: Seoane
authors.forename: L
authors.initials: L
authors.lastname: García-Caballero
authors.forename: P
authors.initials: P
authors.lastname: López-Jornet
authors.forename: T
authors.initials: T
authors.lastname: García-Caballero
authors.forename: P
authors.initials: P
authors.lastname: Varela-Centelles
mesh_terms: D000818:Animals; D016477:Artifacts; D002448:Cell Adhesion; D002466:Cell Nucleolus / Q000473:pathology; D002467:Cell Nucleus / Q000473:pathology; D002843:Chromatin / Q000473:pathology; D003593:Cytoplasm / Q000473:pathology; D004847:Epithelial Cells / Q000473:pathology; D004848:Epithelium / Q000473:pathology / Q000601:surgery; D005927:Glossectomy / Q000295:instrumentation / Q000379:methods*; D006358:Hot Temperature; D007365:Intercellular Junctions / Q000473:pathology; D007633:Keratins; D053685:Laser Therapy / Q000379:methods*; D054020:Lasers, Gas / Q000627:therapeutic use*; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D008853:Microscopy; D008938:Mitosis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014059:Tongue / Q000473:pathology
chemical_list: D002843:Chromatin; D007633:Keratins