title: Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation.
abstract: Mulberroside A was isolated from the ethanol extract of Morus alba roots. The enzymatic hydrolysis of mulberroside A with Pectinex produced oxyresveratrol and oxyresveratrol-3-O-glucoside. We tested oxyresveratrol, oxyresveratrol-3-O-glucoside, and mulberroside A to determine whether they could inhibit ultraviolet B (UVB) irradiation-induced melanogenesis in brown guinea pig skin. Topical application of mulberroside A, oxyresveratrol, and oxyresveratrol-3-O-glucoside reduced the pigmentation in guinea pig skin. These compounds suppressed the expression of melanogenic enzymes tyrosinase, tyrosinase-related protein-1, and microphthalmia transcription factor. The anti-melanogenesis effect was highest with oxyresveratrol, intermediate with oxyresveratrol-3-O-glucoside, and lowest with mulberroside A. Mulberroside A is a glycosylated stilbene of oxyresveratrol; thus, the deglycosylation of mulberroside A resulted in enhanced inhibition of melanogenesis. Histological analysis with Fontana-Masson staining confirmed that these compounds significantly reduced the melanin content in the epidermis of UVB-irradiated guinea pig skin compared to the vehicle control. Thus, these compounds effectively reduced pigmentation and may be suitable cosmetic agents for skin whitening.
authors.affiliation: Department of Biomedical Science, College of Health Science, Korea University, Seoul 136-703, South Korea.
authors.forename: Keun-Tae
authors.initials: KT
authors.lastname: Park
authors.forename: Jeong-Keun
authors.initials: JK
authors.lastname: Kim
authors.forename: Dahyun
authors.initials: D
authors.lastname: Hwang
authors.forename: Yeongmin
authors.initials: Y
authors.lastname: Yoo
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000818:Animals; D004187:Disaccharides / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006168:Guinea Pigs; D008543:Melanins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051739:Microphthalmia-Associated Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D031625:Morus / Q000737:chemistry; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013267:Stilbenes / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D004187:Disaccharides; D005960:Glucosides; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010936:Plant Extracts; D013267:Stilbenes; C420606:mulberroside A; C570979:oxyresveratrol-3-O-glucoside; C034912:puag-haad; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase