title: A proteome reference map of the causative agent of melioidosis Burkholderia pseudomallei.
abstract: Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial gene and environmental regulation and facilitate comparative proteomics with its sibling species.
authors.affiliation: Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. pexcotung@gmail.com
authors.forename: Patompon
authors.initials: P
authors.lastname: Wongtrakoongate
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Sukkid
authors.initials: S
authors.lastname: Yasothornsrikul
authors.forename: Sumalee
authors.initials: S
authors.lastname: Tungpradabkul
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D008554:Melioidosis / Q000382:microbiology*; D011499:Protein Processing, Post-Translational / Q000235:genetics; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012015:Reference Standards; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D020543:Proteome