title: Evaluation of nonpolar metabolites in plant extracts by 13C NMR spectroscopy.
abstract: (13)C nuclear magnetic resonance (NMR) spectroscopy was explored as a simple and efficient technique for the quantitative analysis of nonpolar metabolites in plants. The method was first optimized with a mixture of known metabolites and then applied to the nonpolar leaf extracts of plants harvested in the Valencian community (eastern Spain) belonging to three different genera: Euphorbia (Euphorbiaceae), Araujia (Apocynaceae), and Morus (Moraceae). Furthermore, an exhaustive analysis of  Euphorbia characias  leaf and stem extracts from different geographic locations allowed that quantitative (13)C NMR spectroscopy is a suitable tool for metabolic profiling purpose.
authors.affiliation: Servicio de Resonancia Magnética Nuclear, Centro de Investigación Príncipe Felipe, Avenida Autopista del Saler 16, 46012 Valencia, Spain.
authors.forename: Martina
authors.initials: M
authors.lastname: Palomino-Schätzlein
authors.forename: Pablo V
authors.initials: PV
authors.lastname: Escrig
authors.forename: Herminio
authors.initials: H
authors.lastname: Boira
authors.forename: Jaime
authors.initials: J
authors.lastname: Primo
authors.forename: Antonio
authors.initials: A
authors.lastname: Pineda-Lucena
authors.forename: Nuria
authors.initials: N
authors.lastname: Cabedo
mesh_terms: D029062:Apocynaceae / Q000737:chemistry* / Q000378:metabolism; D028481:Euphorbia / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D031625:Morus / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D013030:Spain
chemical_list: D010936:Plant Extracts