title: Immunofluorescence localization of nuclear proteins.
abstract: Many nuclear proteins have been successfully localized using immunofluorescence microscopy. These proteins span all nuclear domains, including the nuclear envelope, nuclear lamina, nucleolus, chromatin-associated proteins, and proteins associated with RNA metabolism and nuclear bodies. This article describes a general method for localizing nuclear proteins. Cells grown on coverslips are fixed in either formaldehyde or methanol and permeabilized in Triton X-100. Incubating the cells with primary antibody and fluorescently conjugated secondary antibody allows visualization of the target antigen.
authors.forename: David L
authors.initials: DL
authors.lastname: Spector
mesh_terms: D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005557:Formaldehyde / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D009687:Nuclear Proteins / Q000378:metabolism*; D017830:Octoxynol / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D009687:Nuclear Proteins; D013501:Surface-Active Agents; D005557:Formaldehyde; D017830:Octoxynol; D000432:Methanol