title: Preparation of photo-cross-linked small molecule affinity matrices for affinity selection of protein targets for biologically active small molecules.
abstract: Small molecule-immobilized affinity matrices are indispensable tools in chemical genomics to screen and purify protein targets for biologically active small molecules. Usually, prior to immobilization, small -molecules have to be derivatized at a position that does not significantly abrogate the intrinsic biological activity, or chemically synthesized to have an appropriate functional group for the immobilization chemistry. Here, we describe a photo-cross-linking technique to immobilize biologically active small molecules for protein target screening, without the need for chemical derivatization.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takayama
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriya
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanoh
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D016572:Cyclosporine / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D008697:Methane / Q000031:analogs & derivatives / Q000737:chemistry; D008863:Microspheres; D055668:Photochemical Processes; D011506:Proteins / Q000302:isolation & purification*; D012685:Sepharose / Q000737:chemistry; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D011506:Proteins; D054852:Small Molecule Libraries; C030011:carbene; D016572:Cyclosporine; D012685:Sepharose; D008697:Methane