title: Spermatozoa DNA damage measured by sperm chromatin structure assay (SCSA) and birth characteristics in children conceived by IVF and ICSI.
abstract: High levels of spermatozoa DNA damage hinder fertility in vivo but not in vitro. It is a source of worry that following in vitro fertilization (IVF) spermatozoa DNA damage, if not repaired by the oocyte, might have a negative impact on the offspring. The aim of this study was to assess if a high spermatozoa DNA Fragmentation Index (DFI) is associated with alterations in birthweight (BW) and/or gestational length in IVF children. One hundred and thirty-one singleton pregnancies established by standard IVF or intracytoplasmic sperm injection (ICSI) were included in the study. DFI was measured by sperm chromatin structure assay (SCSA) in semen samples used for fertilization. DFI was categorized as low and high, using 20, 30, 40 and 50% as cut-off levels. Birthweight, gestational age, as well as gestational age adjusted BW score were used in a linear regression model as end points For none of the tested birth characteristics, statistically significant differences between the groups with low and high DFI were seen regardless of whether 20, 30, 40 or 50% were used as cut-off levels, both when the IVF and ICSI data were merged or analysed separately. Spermatozoa DNA damage as assessed by SCSA is not associated with BW or gestational length in IVF and ICSI children.
authors.affiliation: Reproductive Medicine Centre, Skanes University Hospital, Malmö, Sweden. mona.bungum@med.lu.se
authors.forename: M
authors.initials: M
authors.lastname: Bungum
authors.forename: L
authors.initials: L
authors.lastname: Bungum
authors.forename: K-F
authors.initials: KF
authors.lastname: Lynch
authors.forename: L
authors.initials: L
authors.lastname: Wedlund
authors.forename: P
authors.initials: P
authors.lastname: Humaidan
authors.forename: A
authors.initials: A
authors.lastname: Giwercman
mesh_terms: D000328:Adult; D001724:Birth Weight / Q000235:genetics; D002843:Chromatin; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro; D005865:Gestational Age; D006801:Humans; D008297:Male; D008875:Middle Aged; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000166:cytology*; D055815:Young Adult
chemical_list: D002843:Chromatin