title: Preferential loading of bergmann glia with synthetic acetoxymethyl calcium dyes.
abstract: The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. This protocol outlines a method for in vivo calcium imaging in cerebellar astrocytes of mice which have undergone a cerebellar craniotomy. Multicell bolus loading (MCBL) is performed using the synthetic calcium indicators Fluo-5F AM and Fluo-4 AM. In the cerebellum, a degree of cell-type specificity can be achieved by varying the depth of injection. This protocol describes a loading procedure following craniotomy which allows preferential labeling of Bergmann glia.
authors.forename: Tycho M
authors.initials: TM
authors.lastname: Hoogland
authors.forename: Bernd
authors.initials: B
authors.lastname: Kuhn
authors.forename: Samuel S-H
authors.initials: SS
authors.lastname: Wang
mesh_terms: D001253:Astrocytes / Q000737:chemistry; D002118:Calcium / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D009457:Neuroglia / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D002118:Calcium