title: Ghrelin potentiates TSH-induced expression of the thyroid tissue-specific genes thyroglobulin, thyroperoxidase and sodium-iodine symporter, in rat PC-Cl3 Cells.
abstract: Ghrelin is a 28-amino-acid peptide that stimulates pituitary growth-hormone secretion and modulates food-intake and energy metabolism in mammals. It is mainly secreted by the stomach, but it is also expressed in many other tissues such as cartilage or the thyroid gland. In the present study we have analyzed by RT-PCR and using immunohistochemistry and immunofluorescence the expression and tissue distribution of ghrelin and its functional receptor (GHS-R type 1α) in thyroid cell-lines and in normal and pathological rat thyroid tissue. Additionally, by measuring the incorporation of BrdU, we have investigated if, as previously noted for FRTL-5 cells, ghrelin enhances the proliferation rate in the PC-Cl3 rat-thyrocyte cell-line. Finally, we have determined the stimulatory effect of ghrelin on TSH-induced expression of the tissue-specific key genes involved in the synthesis of thyroid hormone: thyroglobulin, thyroperoxidase and sodium-iodine symporter. Our data provide direct evidence that C-cell secreted ghrelin may be involved in the paracrine regulation of the thyroid follicular cell function.
authors.affiliation: Department of Cytology and Histology, School of Medicine, University of Seville, 41009 Sevilla, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Morillo-Bernal
authors.forename: J M
authors.initials: JM
authors.lastname: Fernández-Santos
authors.forename: M
authors.initials: M
authors.lastname: De Miguel
authors.forename: R
authors.initials: R
authors.lastname: García-Marín
authors.forename: F
authors.initials: F
authors.lastname: Gordillo-Martínez
authors.forename: E
authors.initials: E
authors.lastname: Díaz-Parrado
authors.forename: J C
authors.initials: JC
authors.lastname: Utrilla
authors.forename: I
authors.initials: I
authors.lastname: Martín-Lacave
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000032:analysis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D054439:Ghrelin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D007150:Immunohistochemistry; D007453:Iodide Peroxidase / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D054440:Receptors, Ghrelin / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters / Q000096:biosynthesis* / Q000235:genetics; D013954:Thyroglobulin / Q000096:biosynthesis* / Q000235:genetics; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology; D000099209:Sodium-Iodide Symporters
chemical_list: D001973:Bromodeoxyuridine; D054439:Ghrelin; D007453:Iodide Peroxidase; D012333:RNA, Messenger; D054440:Receptors, Ghrelin; D027981:Symporters; D013954:Thyroglobulin; D013972:Thyrotropin; D000099209:Sodium-Iodide Symporters