title: Induction, regulation and physiological role of IL-17 secreting helper T-cells isolated from PBMC, thymus, and lung lymphocytes of young pigs.
abstract: Interleukin-17 (IL-17) producing cells, referred to as Th17, have recently emerged as a third subset of the T helper (Th) cell family. Studies in mice have demonstrated that Th17 cells and their associated cytokines are involved in several autoimmune diseases and host defense against infection. Murine Th17 cells differentiate from naïve CD4(+) T-cells in the presence of TGFβ and IL-6, however, there are contradicting reports as to the role of TGFβ in the differentiation of human Th17 cells and very little is known about these cells in other animals. We report here the presence of IL-17 secreting lymphocytes in the lung and peripheral blood of pigs. The cDNA of porcine IL-17 gene was cloned and sequenced from activated lung lymphocytes and PBMC from piglets. A 17kDa recombinant protein was expressed and purified both under denaturing and native conditions from E. coli BL21 cells. Furthermore, we demonstrate that TGFβ in the presence of IL-6 and/or IL-1β induces in vitro differentiation of Th17 cells from naïve porcine CD4(+) thymocytes.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
authors.forename: Tadele G
authors.initials: TG
authors.lastname: Kiros
authors.forename: Jill
authors.initials: J
authors.lastname: van Kessel
authors.forename: Lorne A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: Volker
authors.initials: V
authors.lastname: Gerdts
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D053583:Interleukin-1beta / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D008168:Lung / Q000166:cytology* / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000502:physiology
chemical_list: D020381:Interleukin-17; D053583:Interleukin-1beta; D015850:Interleukin-6; D016212:Transforming Growth Factor beta