title: Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.
abstract: The tandem Tudor-like domain-containing protein Spindlin1 has been reported to be a meiotic spindle-associated protein. Here we report that Spindlin1 is not associated with the spindle in mouse embryonic fibroblast cells during mitotic divisions. In interphase cells, Spindlin1 specifically localizes to the nucleoli. Moreover, Spindlin1 is a histone methylation effector protein that specifically recognizes H3K4 methylation. Finally, Spindlin1 localizes to the active ribosomal DNA (rDNA) repeats, and Spindlin1 facilitates the expression of rRNA genes.
authors.affiliation: Chromatin Lab, National Institute of Biological Sciences, 7 Science Park Road, Zhong Guan Cun Life Science Park, Beijing 102206, China.
authors.forename: Weixiang
authors.initials: W
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Chen
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Mao
authors.forename: Huihui
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ding
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Ruiming
authors.initials: R
authors.lastname: Xu
authors.forename: Bing
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D056426:Genetic Loci / Q000235:genetics; D006367:HeLa Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D012335:RNA, Ribosomal / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D006657:Histones; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D010750:Phosphoproteins; D021122:Protein Subunits; D012335:RNA, Ribosomal; C104434:spindlin; D000090243:Nucleophosmin; D008239:Lysine