title: WT1 expression as a marker of minimal residual disease predicts outcome in acute myeloid leukemia when measured post-consolidation.
abstract: WT1 levels may be a useful predictor of leukemia free survival (LFS) following treatment of acute myeloid leukemia (AML). We report a retrospective study in which levels of WT1 expression from patients with de novo AML were measured from bone marrow and peripheral blood at diagnosis, post-induction, post-consolidation and relapse. We demonstrate that higher levels of WT1 in peripheral blood at diagnosis are associated with poorer LFS independent of age and cytogenetic risk-group (n=85, p=0.028). When measured at post-consolidation, the presence of detectable WT1 is associated with poorer LFS in univariate analysis of both peripheral blood (p=0.024) and bone marrow (p=0.019). In a multivariate analysis including age and cytogenetic risk, the association remained significant for bone marrow (p=0.016) with a trend observed for peripheral blood (p=0.06). These findings have formed the basis for ongoing research.
authors.affiliation: Department of Haematology, The Queen Elizabeth Hospital, Woodville South, SA, Australia.
authors.forename: James X
authors.initials: JX
authors.lastname: Gray
authors.forename: Lyle
authors.initials: L
authors.lastname: McMillen
authors.forename: Peter
authors.initials: P
authors.lastname: Mollee
authors.forename: Sanjoy
authors.initials: S
authors.lastname: Paul
authors.forename: Steven
authors.initials: S
authors.lastname: Lane
authors.forename: Robert
authors.initials: R
authors.lastname: Bird
authors.forename: Devinder
authors.initials: D
authors.lastname: Gill
authors.forename: Russell
authors.initials: R
authors.lastname: Saal
authors.forename: Paula
authors.initials: P
authors.lastname: Marlton
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis*; D060830:Consolidation Chemotherapy; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual / Q000188:drug therapy / Q000378:metabolism* / Q000401:mortality; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025721:WT1 Proteins / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D025721:WT1 Proteins