title: Na+/H+ exchanger 1 gene expression in tissues of yellow chicken.
abstract: The Na(+)/H(+) exchanger 1 (NHE1) transmembrane protein regulates intracellular pH, cell survival, cell growth, cell differentiation and plays a critical role in the progression of some diseases, including the pathogenesis of J avian leukosis. The chicken is an ideal model to study the function of NHE1 because it has developed highly efficient Na(+)-absorptive mechanisms in its small and large intestines. To date, there has been no detailed expression analysis to determine NHE1 expression in various tissues of the chicken. We determined the mRNA and protein expression levels of avian NHE1 by real-time quantitative PCR and immunohistochemical analysis. NHE1 mRNA was detected in all chicken tissues examined. Protein expression levels varied widely among tissues and did not always correlate with mRNA expression. Determining the mRNA and protein of NHE1 expression patterns in chicken should help to delineate the NHE1 role in different tissues and its contribution to physiological and pathological processes. These data provide the basis for examining the distinct function of chicken NHE1 compared with its mammalian counterpart.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
authors.forename: Zhang-yong
authors.initials: ZY
authors.lastname: Ning
authors.forename: Yu-fu
authors.initials: YF
authors.lastname: An
authors.forename: Wen-bao
authors.initials: WB
authors.lastname: Qi
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Jia-qiang
authors.initials: JQ
authors.lastname: Pan
authors.forename: Xin-tao
authors.initials: XT
authors.lastname: Wu
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002060:Bursa of Fabricius / Q000166:cytology / Q000378:metabolism*; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers