title: Assay of amino acid racemases.
abstract: D-Amino acids play important physiological roles in the mammalian body. Recent investigations revealed that, in mammals, D-amino acids are synthesized from their corresponding L-enantiomers via amino acid racemase. This article describes a method used to measure amino acid racemase activity by high-performance liquid chromatography (HPLC). The assay involves fluorogenic chiral derivatization of amino acids with a newly developed reagent, and enantioseparation of D- and L-amino acid derivatives by HPLC. The method is accurate and reliable, and can be automated using a programmable autosampling injector.
authors.affiliation: Department of Pharmaceutical Life Sciences, Kitasato University, Minatoku, Tokyo, Japan.
authors.forename: Masumi
authors.initials: M
authors.lastname: Katane
authors.forename: Masae
authors.initials: M
authors.lastname: Sekine
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Homma
mesh_terms: D000591:Amino Acid Isomerases / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D000591:Amino Acid Isomerases