title: Production of (2S,3S)-2,3-butanediol and (3S)-acetoin from glucose using resting cells of Klebsiella pneumonia and Bacillus subtilis.
abstract: Production of highly pure (2S,3S)-2,3-butanediol ((2S,3S)-2,3-BD) and (3S)-acetoin ((3S)-AC) in high concentrations is desirable but difficult to achieve. In the present study, glucose was first transformed to a mixture of (2S,3S)-2,3-BD and meso-2,3-BD by resting cells of Klebsiella pneumoniae CICC 10011, followed by biocatalytic resolution of the mixture by resting cells of Bacillus subtilis 168. meso-2,3-BD was transformed to (3S)-AC, leaving (2S,3S)-2,3-BD in the reaction medium. Using this approach, 12.5 g l(-1) (2S,3S)-2,3-BD and 56.7 g l(-1) (3S)-AC were produced. Stereoisomeric purity of (2S,3S)-2,3-BD and enantiomeric excess of (3S)-AC was 96.9 and 96.2%, respectively.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Jiayang
authors.initials: J
authors.lastname: Qin
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Dongliang
authors.initials: D
authors.lastname: Hua
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000093:Acetoin / Q000737:chemistry / Q000378:metabolism*; D001412:Bacillus subtilis / Q000166:cytology* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000737:chemistry / Q000378:metabolism*; D002849:Chromatography, Gas; D005240:Feasibility Studies; D005947:Glucose / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000166:cytology* / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; C026978:2,3-butylene glycol; D000093:Acetoin; D005947:Glucose