title: Optical sectioning microscopy with planar or structured illumination.
abstract: A key requirement for performing three-dimensional (3D) imaging using optical microscopes is that they be capable of optical sectioning by distinguishing in-focus signal from out-of-focus background. Common techniques for fluorescence optical sectioning are confocal laser scanning microscopy and two-photon microscopy. But there is increasing interest in alternative optical sectioning techniques, particularly for applications involving high speeds, large fields of view or long-term imaging. In this Review, I examine two such techniques, based on planar illumination or structured illumination. The goal is to describe the advantages and disadvantages of these techniques.
authors.affiliation: Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA. jmertz@bu.edu
authors.forename: Jerome
authors.initials: J
authors.lastname: Mertz
mesh_terms: D000818:Animals; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*