title: Electrophysiological analysis of tetrodotoxin-resistant sodium channel pharmacology.
abstract: This unit describes essential procedures to record the activity of and the effects of pharmacological manipulation upon tetrodotoxin-resistant (TTXr) sodium channels. Readers are guided through the process of assembling a functional |patch-clamp| electrophysiology apparatus and construction of software-controlled voltage protocols that address various aspects of channel gating. Guidelines are also presented for analysis and display of the data typically generated by these experiments. For investigators wishing to study native channels in their appropriate cellular environment, protocols describing the isolation of viable cells from the main tissues that commonly express TTXr channels are also included.
authors.affiliation: Schering-Plough Research Institute, Kenilworth, New Jersey.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Dong
authors.forename: Tony
authors.initials: T
authors.lastname: Priestley
mesh_terms: D000818:Animals; D004351:Drug Resistance; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels / Q000187:drug effects* / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D015222:Sodium Channels; D013779:Tetrodotoxin