title: A new ion-exchange adsorbent with paramagnetic properties for the separation of genomic DNA.
abstract: A new ion-exchange adsorbent (IEA) derived from Fe(3)O(4)/SiO(2)-GPTMS-DEAE with paramagnetic properties was prepared. Fe(3)O(4) nanoparticles were firstly prepared in water-in-oil microemulsion. The magnetic Fe(3)O(4) particles were modified in situ by hydrolysis and condensation reactions with tetraethoxysilane (TEOS) to form the core-shell Fe(3)O(4)/SiO(2). The modified particles were further treated by 3-glycidoxypropyltrimethoxysilane (GPTMS) to form Fe(3)O(4)/SiO(2)-GPTMS nanoparticles. Fe(3)O(4)/SiO(2)-GPTMS-DEAE nanoparticles (IEA) were finally obtained through the condensation reaction between the Cl of diethylaminoethyl chloride-HCl (DEAE) and the epoxy groups of GPTMS in the Fe(3)O(4)/SiO(2)-GPTMS. The obtained IEA has features of paramagnetic and ion exchange properties because of the Fe(3)O(4) nanoparticles and protonated organic amine in the sample. The intermediates and final product obtained in the synthesis process were characterized. The separation result of genomic DNA from blood indicated that Fe(3)O(4)/SiO(2)-GPTMS-DEAE nanoparticles have outstanding advantages in operation, selectivity, and capacity.
authors.affiliation: College of Chemistry & Materials Science, Shaanxi Normal University, Xi'an, P.R. China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Feng
authors.forename: Luan
authors.initials: L
authors.lastname: Jiang
authors.forename: Puhong
authors.initials: P
authors.lastname: Wen
authors.forename: Yali
authors.initials: Y
authors.lastname: Cui
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Daodao
authors.initials: D
authors.lastname: Hu
mesh_terms: D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004247:DNA / Q000097:blood / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004983:Ethanolamines / Q000737:chemistry; D016678:Genome / Q000235:genetics*; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: C403136:3-glycidoxypropyltrimethoxysilane; D004983:Ethanolamines; D058185:Magnetite Nanoparticles; D012821:Silanes; D012822:Silicon Dioxide; D004247:DNA; C007369:2-diethylaminoethanol