title: Serological characterizations of tandem repeat proteins for detection of African trypanosome infection in cattle.
abstract: Serological diagnosis is a useful method to detect African trypanosome infection in livestock animals. Currently available serological tests utilize whole parasites or crude antigens, and recombinant antigens may improve reproducibility/standardization and reduce production costs. With a goal of identifying such recombinant proteins, we computationally identified proteins with tandem repeat (TR) domain from the parasite proteomes and evaluated their potential for serological diagnosis of African trypanosome infections in cattle. Among those tested, Tbg4 demonstrated the best performance with 92% sensitivity, followed by TbbGM6 (85%), TcoGM6 (85%), Tbg2 (65%) and Tbg5 (65%). Although further evaluations such as investigating cross-reactivity to other infections are needed, our data indicate the potential of these antigens for detection of African trypanosome infection in cattle.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan. aygoto@mail.ecc.u-tokyo.ac.jp
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Goto
authors.forename: Malcolm S
authors.initials: MS
authors.lastname: Duthie
authors.forename: Thu-Thuy
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Masahito
authors.initials: M
authors.lastname: Asada
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Kawazu
authors.forename: Darrick
authors.initials: D
authors.lastname: Carter
authors.forename: Noboru
authors.initials: N
authors.lastname: Inoue
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015203:Reproducibility of Results; D012698:Serologic Tests; D020080:Tandem Repeat Sequences; D014353:Trypanosomiasis, African / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins