title: Expression of zona pellucida B proteins in juvenile rare minnow (Gobiocypris rarus) exposed to 17α-ethinylestradiol, 4-nonylphenol and bisphenol A.
abstract: Zona pellucida (ZP) containing proteins are glycoproteins in teleost chorion and are encoded by several gene subfamilies, mainly including ZPA, ZPB, ZPC and ZPX genes. In teleost species, ZP genes are expressed either in liver under regulation of estrogen or in ovary. In the present study, five ZP gene isoforms were isolated and characterized in Gobiocypris rarus. The putative amino acid sequences of these ZP gene isoforms contain the typical trefoil motif and a ZP domain. These five G. rarus ZP gene isoforms were named as grZPB.1, grZPB.2, grZPB.3, grZPB.4 and grZPB.5. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis indicated that all these ZP mRNA isoforms were exclusively expressed in ovary. G. rarus juveniles at the age of 21 days postfertilization were exposed to 17α-ethinylestradiol (EE2; 0.01, 0.1 and 1 nM), 4-nonylphenol (4-NP; 10, 100 and 1000 nM) or bisphenol A (BPA; 0.1, 1 and 10nM) for 3 days. mRNA expressions of ZPB isoforms following the exposure to xenoestrogen were detected by RT-qPCR. Data were analyzed by the 2(-△△Cq) method. The results indicate that induction by 0.1-1nM EE2 on mRNA expression of the grZPB isoforms is weaker than for vitellogenin. 4-NP exposures at three concentrations had differential effects on the grZPBs. BPA at three concentrations weakly induced mRNA expression of the grZPB isoforms.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Wu
authors.forename: Houpeng
authors.initials: H
authors.lastname: Wang
authors.forename: Fang
authors.initials: F
authors.lastname: Qin
authors.forename: Shaozhen
authors.initials: S
authors.lastname: Liu
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001559:Benzhydryl Compounds; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000196:embryology / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004527:Egg Proteins / Q000145:classification / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D004967:Estrogens / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000633:toxicity; D005260:Female; D029941:Fish Proteins / Q000235:genetics*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D010636:Phenols / Q000633:toxicity; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015262:Xenobiotics / Q000633:toxicity; D000072216:Zona Pellucida Glycoproteins; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004527:Egg Proteins; D004967:Estrogens; D004997:Ethinyl Estradiol; D029941:Fish Proteins; D008562:Membrane Glycoproteins; D010636:Phenols; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D015262:Xenobiotics; D000072216:Zona Pellucida Glycoproteins; C041594:4-nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds