title: [Serological detection of Cryptosporidium spp. infection in outpatients in Changchun].
abstract: CP23 gene of Cryptosporidium parvum was expressed in Escherichia coli, and the recombinant protein was purified. Its immunoreactivity was analyzed by Western blotting. Serum samples were collected from outpatients of different ages from August to November, 2010 in Changchun. Indirect ELISA was established to detect the anti-CP23 IgG in sera. Western blotting analysis indicated that the recombinant CP23 protein was recognized by sera from Cryptosporidium panum-infected calves and positive human sera, but not recognized by sera of mice infected with Schistosoma japonicum, sera from falciparum malaria patients and negative human sera. The overall anti-CP23 IgG positive rate was 3.2% (65/2 046). The seropositive rate was 2.7% (28/1 036) in men and 3.7% (37/1 010) in women (P > 0.05). The seropositive rates were significantly different among age groups (P < 0.05), and the age group of 71-80 had the highest positive rate (8.6%, 13/152).
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education Changchun 130062, China.
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Song
authors.forename: Ji-Xue
authors.initials: JX
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hong-Xia s
authors.initials: HX
authors.lastname: Yue
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Ji-Gang
authors.initials: JG
authors.lastname: Yin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000097:blood / Q000175:diagnosis*; D003458:Cryptosporidium / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012698:Serologic Tests; D055815:Young Adult
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G