title: Overexpression of the immunoreceptor CD300f has a neuroprotective role in a model of acute brain injury.
abstract: It is well known that cell surface immune receptors play a critical role in regulating immune and inflammatory processes in the central nervous system (CNS). We have analyzed the function of cluster of differentiation (CD)300f immunoreceptor in a model of excitotoxic rat brain damage. First, to explore the presence of endogenous ligand(s) for this receptor we used a human CD300f-Ig soluble protein and confocal microscopy, showing specific staining mainly in CNS white matter and on the surface of oligodendrocytes and certain astrocytes. Next, we demonstrated in a model of in vivo rat brain excitotoxic damage that the overexpression of human CD300f induced a significant reduction in the lesion volume. To validate these results, we cloned the rat ortholog of CD300f protein (rCD300f). The overexpression of rCD300f receptor had a comparable neuroprotective effect after the acute brain injury and a similar CNS staining pattern when stained with the rCD300f-Ig soluble protein. Interestingly, when we analyzed the expression pattern of rCD300f in brain cells by quantitative polymerase chain reaction and immunohistochemistry, we detected the expression of CD300f as expected in microglial cells, but also in oligodendrocytes and neurons. These data suggest that the neuroprotective role of CD300f would be the result of a complex network of cell interactions.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, UDELAR, Montevideo, Uruguay. hugo.peluffo@pasteur.edu.uy
authors.forename: Hugo
authors.initials: H
authors.lastname: Peluffo
authors.forename: Daniela
authors.initials: D
authors.lastname: Alí-Ruiz
authors.forename: Aroa
authors.initials: A
authors.lastname: Ejarque-Ortíz
authors.forename: Victor
authors.initials: V
authors.lastname: Heras-Alvarez
authors.forename: Emma
authors.initials: E
authors.lastname: Comas-Casellas
authors.forename: Agueda
authors.initials: A
authors.lastname: Martínez-Barriocanal
authors.forename: Andres
authors.initials: A
authors.lastname: Kamaid
authors.forename: Damiana
authors.initials: D
authors.lastname: Alvarez-Errico
authors.forename: Maria Luciana
authors.initials: ML
authors.lastname: Negro
authors.forename: Natalia
authors.initials: N
authors.lastname: Lago
authors.forename: Simó
authors.initials: S
authors.lastname: Schwartz
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
authors.forename: Joan
authors.initials: J
authors.lastname: Sayós
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D006801:Humans; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009836:Oligodendroglia / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*
chemical_list: C519443:CD300LF protein, human; D011971:Receptors, Immunologic