title: Quality control of Citri reticulatae pericarpium: exploratory analysis and discrimination.
abstract: Extracts of Citri reticulatae pericarpium (PCR) are commonly used in the Traditional Chinese Medicine. The quality control of PCR is currently performed by single marker analysis, which can hardly describe the complexity of such natural samples. In this study, a fingerprint methodology for PCR based on high-performance liquid chromatography (HPLC) was developed and validated. A total of 69 fingerprints of authenticated PCR samples, commercial PCR samples, mixed peel samples, and other Citrus peels were recorded. Exploratory data analysis allowed optimizing the extraction procedure and detecting mixed peel samples. Once the optimizations were performed and the method validated, discrimination between the authentic PCR samples and all other samples was performed by p-Discriminant Partial Least Squares. The established model was able to differentiate between classes with a high reliability for each sample. Furthermore, evaluation of the score and loading plots of the model indicated nobiletin, tangeretin, naringin and hesperidin as important markers for the quality control of PCR.
authors.affiliation: Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium.
authors.forename: Christophe
authors.initials: C
authors.lastname: Tistaert
authors.forename: Line
authors.initials: L
authors.lastname: Thierry
authors.forename: Andrzej
authors.initials: A
authors.lastname: Szandrach
authors.forename: B
authors.initials: B
authors.lastname: Dejaegher
authors.forename: Guorong
authors.initials: G
authors.lastname: Fan
authors.forename: Michel
authors.initials: M
authors.lastname: Frédérich
authors.forename: Yvan
authors.initials: Y
authors.lastname: Vander Heyden
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D016002:Discriminant Analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis; D047309:Flavones / Q000032:analysis; D006569:Hesperidin / Q000032:analysis; D016018:Least-Squares Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal; D044950:Flavanones; D047309:Flavones; C008661:nobiletin; D006569:Hesperidin; C059006:tangeretin; C005274:naringin