title: A discussion of molecular biology methods for protein engineering.
abstract: A number of molecular biology techniques are available to generate variants from a particular start gene for eventual protein expression. We discuss the basic principles of these methods in a repertoire that may be used to achieve the elemental steps in protein engineering. These include site-directed, deletion and insertion mutagenesis. We provide detailed case studies, drawn from our own experiences, packaged together with conceptual discussions and include an analysis of the techniques presented with regards to their uses in protein engineering.
authors.affiliation: Gene Expression and Biophysics Group, Synthetic Biology-ERA, CSIR Biosciences, Brummeria, Pretoria 0001, South Africa. azawaira@gmail.com
authors.forename: Alexander
authors.initials: A
authors.lastname: Zawaira
authors.forename: Anil
authors.initials: A
authors.lastname: Pooran
authors.forename: Samantha
authors.initials: S
authors.lastname: Barichievy
authors.forename: Denis
authors.initials: D
authors.lastname: Chopera
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D015202:Protein Engineering / Q000379:methods*
chemical_list: D017931:DNA Primers