title: A versatile and highly sensitive probe for Hg(II), Pb(II) and Cd(II) detection individually and totally in water samples.
abstract: The detection of heavy metal ions using enzyme-linked immunosorbent assays (ELISA) has been reported by several research groups. However, highly sensitive and selective detection of total heavy metal ions using ELISA is a major technical limitation. Here we describe the development of a versatile and highly sensitive probe combining goat anti-mice IgG, colloidal gold nanoparticles (AuNPs) and horseradish peroxidase (HRP). We demonstrate the utility of this probe using three kinds of heavy metal complete antigens and three monoclonal antibodies (McAbs) in one ELISA system to establish a high-throughput screening protocol. The procedure was successfully applied to analysis of Hg(II), Pb(II) and Cd(II) individually and totally from different water samples. The sensitivities for the detection of Hg(II), Pb(II) and Cd(II) individually and totally are 27.4, 3.9, 15.8 and 18.2 nM, respectively. And all limit of detection (LODs) are lower than 1.2 nM. The recovery results obtained from the developed technique showed a good correlation (R(2)=0.983) with those from ICP-MS. The major advantage of the probe is the versatility and high sensibility. The probe could be potentially used, upon demand, as a sensitive and versatile detector for a broad range of applications.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. zhouyu69@sina.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Tian
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Li
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xin-Rui
authors.initials: XR
authors.lastname: Wang
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Ren
authors.forename: Zeng-Shan
authors.initials: ZS
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019216:Metals, Heavy / Q000032:analysis*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D014867:Water