title: LC-ESI-MS/MS determination of paclitaxel on dried blood spots.
abstract: A simple and rapid high-performance liquid chromatography-tandem mass spectrometric assay for determination of paclitaxel on rat dried blood spots was developed and validated. The extracted sample was chromatographed without further treatment using a reverse-phase Oyster ODS3, 4.6 × 50 mm, 3 µm column with mass spectrometry detection. The mobile phase comprised of acetonitrile-water, 60:40 v/v, with a flow rate of 0.4 mL/min was used. The calibration was linear over the range 0.2-20 ng/mL. The limits of detection and quantification were 0.08 and 0.2 ng/mL, respectively. The intra- and inter-day precision (CV%) and accuracy (relative error %) were less than 10 and 12%, respectively.
authors.affiliation: Analytical Chemistry Division, Indian Institute of Chemical Technology, Tarnaka, Hyderabad-500607, India. rnrao55@yahoo.com
authors.forename: R
authors.initials: R
authors.lastname: Nageswara Rao
authors.forename: S
authors.initials: S
authors.lastname: Satyanarayana Raju
authors.forename: R
authors.initials: R
authors.lastname: Mastan Vali
authors.forename: V U M
authors.initials: VU
authors.lastname: Sarma
authors.forename: G
authors.initials: G
authors.lastname: Girija Sankar
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D000077143:Docetaxel; D059788:Dried Blood Spot Testing / Q000379:methods*; D004355:Drug Stability; D057230:Limit of Detection; D016014:Linear Models; D017239:Paclitaxel / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D043823:Taxoids / Q000097:blood
chemical_list: D043823:Taxoids; D000077143:Docetaxel; D017239:Paclitaxel