title: An improved non-enzymatic "DNA ladder assay" for more sensitive and early detection of apoptosis.
abstract: Conventional DNA ladder assay has certain shortcomings such as loss of DNA fragments during sample processing, involvement of multiple steps and requirement of expensive reagents. The present study demonstrates a rapid, easy-to-perform cost-effective method for detection of apoptotic DNA fragments with considerable improvement in the sensitivity by avoiding loss of DNA fragments. It involves a few minutes of procedure involving direct lysis of cells with dimethyl sulphoxide (DMSO), brief vortexing, addition of 2% SDS-TE buffer, and a single step of centrifugation. This cost- and time-efficient method reduces the assay time considerably and can be used for a large number of samples with excellent sensitivity.
authors.affiliation: Natural Radiation Response Mechanisms (NRRM) Group, Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences (INMAS), Brig. S. K. Majumdar Road, Timarpur, Delhi, 110054, India.
authors.forename: Shubhankar
authors.initials: S
authors.lastname: Suman
authors.forename: Akshay
authors.initials: A
authors.lastname: Pandey
authors.forename: Sudhir
authors.initials: S
authors.lastname: Chandna