title: Inhibition of Lassa virus and Ebola virus infection in host cells treated with the kinase inhibitors genistein and tyrphostin.
abstract: Arenaviruses and filoviruses are capable of causing hemorrhagic fever syndrome in humans. Limited therapeutic and/or prophylactic options are available for humans suffering from viral hemorrhagic fever. In this report, we demonstrate that pre-treatment of host cells with the kinase inhibitors genistein and tyrphostin AG1478 leads to inhibition of infection or transduction in cells infected with Ebola virus, Marburg virus, and Lassa virus. In all, the results demonstrate that a kinase inhibitor cocktail consisting of genistein and tyrphostin AG1478 is a broad-spectrum antiviral that may be used as a therapeutic or prophylactic against arenavirus and filovirus hemorrhagic fever.
authors.affiliation: Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555, USA.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Kolokoltsov
authors.forename: Shramika
authors.initials: S
authors.lastname: Adhikary
authors.forename: Jennifer
authors.initials: J
authors.lastname: Garver
authors.forename: Lela
authors.initials: L
authors.lastname: Johnson
authors.forename: Robert A
authors.initials: RA
authors.lastname: Davey
authors.forename: Eric M
authors.initials: EM
authors.lastname: Vela
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D029043:Ebolavirus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D019833:Genistein / Q000494:pharmacology*; D019142:Hemorrhagic Fever, Ebola / Q000188:drug therapy / Q000821:virology*; D006801:Humans; D007835:Lassa Fever / Q000188:drug therapy / Q000821:virology*; D007836:Lassa virus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011799:Quinazolines / Q000494:pharmacology*; D020032:Tyrphostins / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D047428:Protein Kinase Inhibitors; D011799:Quinazolines; D020032:Tyrphostins; C101044:RTKI cpd; D019833:Genistein