title: Nanocarriers for cytoplasmic delivery: cellular uptake and intracellular fate of chitosan and hyaluronic acid-coated chitosan nanoparticles in a phagocytic cell model.
abstract: The cellular uptake of hyaluronic-acid-coated, negatively charged chitosan/triphosphate nanoparticles and that of uncoated, positively charged ones is investigated by studying cellular localization, uptake kinetics and mechanism of internalization in J774.2 macrophages, using non-phagocytic L929 fibroblasts as a control for uncoated nanoparticles. Both kinds of nanoparticles undergo endosomal escape and adopt a similar clathrin-based endocytic mechanism. The surface decoration with HA profoundly influences the kinetics of cellular uptake, with an at least two orders of magnitude slower kinetics, but also the nature of the binding on the cellular surface.
authors.affiliation: Department of Pharmaceutics, Faculty of Pharmacy, Ain Shams University, Monazamet El Wehda El Afrikia St., El Abbassia, Cairo, Egypt.
authors.forename: Noha M
authors.initials: NM
authors.lastname: Zaki
authors.forename: Alessandro
authors.initials: A
authors.lastname: Nasti
authors.forename: Nicola
authors.initials: N
authors.lastname: Tirelli
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D011992:Endosomes / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D010316:Particle Size; D055672:Static Electricity
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D006820:Hyaluronic Acid; D048271:Chitosan