title: Apolipoprotein A-I peptide models as probes to formulate potential inhibitors of the low-density lipoprotein oxidation.
abstract: Apolipoprotein A-I (apoA-I), which constitutes the principal protein component of high-density lipoprotein, is responsible for its major antiatherogenic functions. Aiming at contributing to the development of potent inhibitors of low-density lipoprotein (LDL) peptide models of helices 4,6 and 9,10 of apoA-I were designed and synthesized. Specific amino acid substitutions, resulting in transformation of the original helix class A and Y to G according to the Schiffer and Edmundson helical wheel representation, were introduced in order to validate the contribution of these modifications in the inhibitory activity of the synthesized peptide models against the LDL oxidation. The role of Met at positions 112 (helix 4) and 148 (helix 6) as oxidant scavenger was also investigated. The helical characteristics of all the peptide models were studied by CD in membrane-mimicking microenvironments and compared with the original helices.
authors.affiliation: Department of Medicine, Division of Cardiology, New York University School of Medicine, USA.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Darvari
authors.forename: Maria P
authors.initials: MP
authors.lastname: Petraki
authors.forename: Constantinos
authors.initials: C
authors.lastname: Tellis
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Harilogis
authors.forename: Alexandros D
authors.initials: AD
authors.lastname: Tselepis
authors.forename: Maria
authors.initials: M
authors.lastname: Sakarellos-Daitsiotis
mesh_terms: D000595:Amino Acid Sequence; D016632:Apolipoprotein A-I / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors* / Q000737:chemistry; D008956:Models, Chemical; D015335:Molecular Probes; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D016632:Apolipoprotein A-I; D008077:Lipoproteins, LDL; D015335:Molecular Probes; D010455:Peptides