title: Proteome studies on liver tissue in a phenobarbital-induced rat model.
abstract: Many drugs may affect the activity of cytochrome P450 (CYP), which is a major source of adverse drug interactions (ADR). Phenobarbital (PB) is the typical inducer of cytochrome P450. The aim of our study was to determine the changes in the cytosolic proteins expression in rat liver at a protein level following induction of cytochrome P450. Firstly, we made a phenobarbital-induced cytochrome P450 rat model. The total cytosolic proteins were then extracted from rat liver tissue and separated by 2-D gel electrophoresis (2-DE). Differentially expressed spots were identified by MALDI-TOF/TOF tandem mass spectrometry followed by database searching. Eight differentially expressed proteins were identified and these proteins were found to be involved in protein degradation, oxidative stress, energy metabolism, biotransformation, and the synthesis of quinolinic acid (QUIN). These findings should provide useful information for research into the regulation of cytochrome P450 gene expression, drug metabolism and drug interaction.
authors.affiliation: National Key Research laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.forename: Yong-He
authors.initials: YH
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Wan-Lu
authors.initials: WL
authors.lastname: Shi
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004569:Electroencephalography / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D010634:Phenobarbital / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital