title: Differential induction, purification and characterization of cold active lipase from Yarrowia lipolytica NCIM 3639.
abstract: The production, purification and characterization of cold active lipases by Yarrowia lipolytica NCIM 3639 is described. The study presents a new finding of production of cell bound and extracellular lipase activities depending upon the substrate used for growth. The strain produced cell bound and extracellular lipase activity when grown on olive oil and Tween 80, respectively. The organism grew profusely at 20 °C and at initial pH of 5.5, producing maximum extracellular lipase. The purified lipase has a molecular mass of 400 kDa having 20 subunits forming a multimeric native protein. Further the enzyme displayed an optimum pH of 5.0 and optimum temperature of 25 °C. Peptide mass finger printing reveled that some peptides showed homologues sequence (42%) to Yarrowia lipolytica LIP8p. The studies on hydrolysis of racemic lavandulyl acetate revealed that extracellular and cell bound lipases show preference over the opposite antipodes of irregular monoterpene, lavandulyl acetate.
authors.affiliation: NCIM Resource Center, National Chemical Laboratory (CSIR), Maharashtra, Pune 411008, India.
authors.forename: K N Sathish
authors.initials: KN
authors.lastname: Yadav
authors.forename: M G
authors.initials: MG
authors.lastname: Adsul
authors.forename: K B
authors.initials: KB
authors.lastname: Bastawde
authors.forename: D D
authors.initials: DD
authors.lastname: Jadhav
authors.forename: H V
authors.initials: HV
authors.lastname: Thulasiram
authors.forename: D V
authors.initials: DV
authors.lastname: Gokhale
mesh_terms: D000085:Acetates / Q000378:metabolism; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000201:enzymology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007477:Ions; D007700:Kinetics; D008049:Lipase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D013058:Mass Spectrometry; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D039821:Monoterpenes / Q000378:metabolism; D009596:Nitrophenols / Q000378:metabolism; D000069463:Olive Oil; D009943:Organophosphorus Compounds / Q000378:metabolism; D010449:Peptide Mapping; D010938:Plant Oils / Q000737:chemistry; D011136:Polysorbates / Q000494:pharmacology; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D025062:Yarrowia / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000085:Acetates; D007477:Ions; D008670:Metals; D039821:Monoterpenes; D009596:Nitrophenols; D000069463:Olive Oil; D009943:Organophosphorus Compounds; D010938:Plant Oils; D011136:Polysorbates; C008644:nitrophenylphosphate; D008049:Lipase; C492922:lavandulyl acetate