title: The abnormal phenotypes of cartilage and bone in calcium-sensing receptor deficient mice are dependent on the actions of calcium, phosphorus, and PTH.
abstract: Patients with neonatal severe hyperparathyroidism (NSHPT) are homozygous for the calcium-sensing receptor (CaR) mutation and have very high circulating PTH, abundant parathyroid hyperplasia, and severe life-threatening hypercalcemia. Mice with homozygous deletion of CaR mimic the syndrome of NSHPT. To determine effects of CaR deficiency on skeletal development and interactions between CaR and 1,25(OH)(2)D(3) or PTH on calcium and skeletal homeostasis, we compared the skeletal phenotypes of homozygous CaR-deficient (CaR(-/-)) mice to those of double homozygous CaR- and 1α(OH)ase-deficient [CaR(-/-)1α(OH)ase(-/-)] mice or those of double homozygous CaR- and PTH-deficient [CaR(-/-)PTH(-/-)] mice at 2 weeks of age. Compared to wild-type littermates, CaR(-/-) mice had hypercalcemia, hypophosphatemia, hyperparathyroidism, and severe skeletal growth retardation. Chondrocyte proliferation and PTHrP expression in growth plates were reduced significantly, whereas trabecular volume, osteoblast number, osteocalcin-positive areas, expression of the ALP, type I collagen, osteocalcin genes, and serum ALP levels were increased significantly. Deletion of 1α(OH)ase in CaR(-/-) mice resulted in a longer lifespan, normocalcemia, lower serum phosphorus, greater elevation in PTH, slight improvement in skeletal growth with increased chondrocyte proliferation and PTHrP expression, and further increases in indices of osteoblastic bone formation. Deletion of PTH in CaR(-/-) mice resulted in rescue of early lethality, normocalcemia, increased serum phosphorus, undetectable serum PTH, normalization in skeletal growth with normal chondrocyte proliferation and enhanced PTHrP expression, and dramatic decreases in indices of osteoblastic bone formation. Our results indicate that reductions in hypercalcemia play a critical role in preventing the early lethality of CaR(-/-) mice and that defects in endochondral bone formation in CaR(-/-) mice result from effects of the marked elevation in serum calcium concentration and the decreases in serum phosphorus concentration and skeletal PTHrP levels, whereas the increased osteoblastic bone formation results from direct effects of PTH.
authors.affiliation: State Key Laboratory of Reproductive Medicine, The Research Center for Bone and Stem Cells, Department of Anatomy, Histology, and Embryology, Nanjing Medical University, Nanjing, China.
authors.forename: Jingning
authors.initials: J
authors.lastname: Liu
authors.forename: Fangqiao
authors.initials: F
authors.lastname: Lv
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Chunxiang
authors.initials: C
authors.lastname: Tao
authors.forename: Guoxian
authors.initials: G
authors.lastname: Ding
authors.forename: Andrew
authors.initials: A
authors.lastname: Karaplis
authors.forename: Edward
authors.initials: E
authors.lastname: Brown
authors.forename: David
authors.initials: D
authors.lastname: Goltzman
authors.forename: Dengshun
authors.initials: D
authors.lastname: Miao
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000002:abnormalities* / Q000378:metabolism; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002356:Cartilage / Q000002:abnormalities* / Q000378:metabolism; D049109:Cell Proliferation; D019902:Chondrocytes / Q000378:metabolism; D006720:Homozygote; D006961:Hyperparathyroidism / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D010006:Osteoblasts / Q000378:metabolism*; D010012:Osteogenesis / Q000235:genetics; D010281:Parathyroid Hormone / Q000097:blood / Q000235:genetics*; D010758:Phosphorus / Q000097:blood / Q000378:metabolism*; D044169:Receptors, Calcium-Sensing / Q000172:deficiency / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics / Q000378:metabolism
chemical_list: D010281:Parathyroid Hormone; D044169:Receptors, Calcium-Sensing; D010758:Phosphorus; D013250:Steroid Hydroxylases; C406322:vitamin D 1-alpha hydroxylase; D002118:Calcium