title: Origin of dissolved-phase hyperpolarized 129Xe signal in the mouse chest based on experimental evidence from extensive magnetic resonance measurements.
abstract: Pulmonary study using hyperpolarized (HP) (129)Xe gas as an imaging medium must focus on dissolved-phase signals to make the most of the characteristic affinity of xenon for biological tissues, including blood. However, the spectral pattern of these signals differs between mice and other animals, including rats, canines, and humans. Dissolved-phase study has been reported only scarcely in mice, so spectral assignment has been an important subject for HP (129)Xe magnetic resonance (MR) spectroscopy (MRS) and MR imaging for its wider application. We performed MRS, including magnetization transfer experiments, and MR imaging studies to confirm the origin of dissolved-phase signals of mice ex vivo and in vivo and obtained evidence to assign dissolved-phase signals at 192 ppm for epicardial fat, 196 ppm for lung parenchyma, and 200 ppm for blood. These results were the first to show the possibility of fast exchange of xenon between plasma and red blood cells.
authors.affiliation: Department of Medical Physics and Engineering, Division of Health Sciences, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Narazaki
authors.forename: Atsuomi
authors.initials: A
authors.lastname: Kimura
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Wakayama
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Imai
authors.forename: Hideaki
authors.initials: H
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D051381:Rats; D013909:Thorax; D014979:Xenon Isotopes / Q000493:pharmacokinetics*
chemical_list: D014979:Xenon Isotopes