title: BRAF(V600E) mutation analysis from May-Grünwald Giemsa-stained cytological samples as an adjunct in identification of high-risk papillary thyroid carcinoma.
abstract: The BRAF(V600E) mutation is specific for thyroid papillary cancer (PTC) and correlates with PTCs invasiveness. This study investigated whether detection of BRAF(V600E) mutation can be performed on routinely stained FNABs. We also examined if establishment of the BRAF(V600E) mutation could help in identification of patients at higher risk for metastatic disease. DNA was isolated from 134 FNABs samples (20 follicular neoplasm, ten suspicious for malignancy, and 104 malignant) using Pinpoint Slide DNA Isolation System. BRAF(V600E) mutation was detected by PCR followed by sequencing. DNA was successfully extracted from all examined FNABs samples. In follicular neoplasm, suspicious for malignancy and malignant FNABs, BRAF(V600E) mutation was found in 0/20 (0%), 2/10 (20%), and 47/104 (45.2%) of cases, respectively. Extra-thyroidal extension was detected in 35/47 (74.4%) BRAF(V600E) positive and in 24/57 (42.1%) wild-type BRAF cases (p = 0.001). Metastases were detected in 37/47 (78.7%) BRAF(V600E) positive and in 28/57 (49.1%) wild-type BRAF cases (p = 0.002). Our results showed that stained FNAB specimens can be used for DNA extraction and assessment of BRAF(V600E) mutation. Detection of BRAF(V600E) mutation had limited value in diagnoses of malignancy in follicular neoplasms but can ascertain malignancy in subset of suspicious for malignancy FNABs. In malignant FNABs, BRAF(V600E) mutation was significantly associated with presence of extra-thyroidal extension and metastases after surgery.
authors.affiliation: Department of Pediatrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814-4712, USA.
authors.forename: Aneeta
authors.initials: A
authors.lastname: Patel
authors.forename: Joanna
authors.initials: J
authors.lastname: Klubo-Gwiezdzinska
authors.forename: Victoria
authors.initials: V
authors.lastname: Hoperia
authors.forename: Alexander
authors.initials: A
authors.lastname: Larin
authors.forename: Kirk
authors.initials: K
authors.lastname: Jensen
authors.forename: Andrew
authors.initials: A
authors.lastname: Bauer
authors.forename: Vasyl
authors.initials: V
authors.lastname: Vasko
mesh_terms: D044963:Biopsy, Fine-Needle / Q000379:methods; D018265:Carcinoma, Papillary, Follicular / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D014644:Genetic Variation; D006801:Humans; D008751:Methylene Blue / Q000737:chemistry; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm; C043260:May-Grunwald Giemsa; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)