title: Genetic and  epigenetic status of triple exotic consanguinity cotton introgression lines.
abstract: Introgression  lines are some of the most important germplasm for breeding applications and  other research conducted on cotton crops. The DNA methylation level among 10  introgression lines of cotton (Gossypium hirsutum) and three exotic  parental species (G. arboreum, G. thurberi and G. barbadense)  were assessed by methylation-sensitive amplified polymorphism (MSAP)  technology. The methylation level in the introgression lines ranged from 33.3  to 51.5%. However, the lines PD0111 and PD0113 had the lowest methylation level  (34.6 and 33.3%, respectively) due to demethylation of most non-coding  sequences. Amplified fragment length polymorphism (AFLP) was used to evaluate  the genetic polymorphism in the cotton introgression lines. A high degree of  polymorphism was observed in all introgression lines (mean 47.2%) based on AFLP  and MSAP analyses. This confirmed the effects of genetic improvement on cotton  introgression lines. The low methylation varieties, PD0111 and PD0113  (introgression lines), clustered outside of the introgression lines based on  MSAP data, which was incongruent with an AFLP-based dendrogram. This phenomenon  could be caused by environmental changes or introgression of exotic DNA  fragments.
authors.affiliation: State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
authors.forename: S P
authors.initials: SP
authors.lastname: He
authors.forename: J L
authors.initials: JL
authors.lastname: Sun
authors.forename: X M
authors.initials: XM
authors.lastname: Du
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D019175:DNA Methylation; D018744:DNA, Plant / Q000378:metabolism; D057890:Epigenomics; D014644:Genetic Variation; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development*; D007178:Inbreeding; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant