title: Molecular characterization, polymorphism of the ACOX1 gene and association with ultrasound traits in Bos taurus.
abstract: Acyl-coenzyme  A oxidase 1 (ACOX1) is the first enzyme in peroxisomal fatty acid β-oxidation;  it is rate-limiting and plays a key role in fatty acid metabolism and fat  deposition. ACOX1 is an important candidate gene for meat quality selection  through marker-assisted selection. Genomic structural analysis showed that  bovine ACOX1 shares 86% identity with human ACOX1. Using PCR-SSCP technology,  we discovered a single nucleotide polymorphism (SNP) (A1865C) in exon 13 of the  ACOX1 gene. Allele frequencies of this SNP were investigated and evaluated with  the χ(2) test in 641 cattle  populations; only the Jiaxian red population was not in Hardy-Weinberg  equilibrium. Gene heterozygosity, effective allele numbers and polymorphism  information content of the bovine ACOX1 locus in seven populations varied from  0.2778 to 0.4954, 1.3846 to 1.9817 and 0.2392 to 0.3727, respectively. We also  looked for a potential association of this SNP with ultrasound traits in 327  individuals and found a significant effect on ultrasound backfat thickness and  ultrasound marbling score (P < 0.05). Meat quality traits were analyzed in  another 71 Qinchuan individuals to determine associations with genotype. Animals  with genotype AA had higher mean values of backfat thickness than those with  genotypes AC and CC. A represents the base before mutation and C represents the  base after mutation. We conclude that this SNP of the ACOX1 gene has potential  as a genetic marker for meat quality traits in cattle reproduction and breeding.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiao
authors.forename: L S
authors.initials: LS
authors.lastname: Zan
authors.forename: Y F
authors.initials: YF
authors.lastname: Liu
authors.forename: H B
authors.initials: HB
authors.lastname: Wang
mesh_terms: D042962:Acyl-CoA Oxidase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D005227:Fatty Acids / Q000378:metabolism; D005787:Gene Frequency; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D006579:Heterozygote; D008460:Meat; D008461:Meat Products; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014463:Ultrasonography
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D042962:Acyl-CoA Oxidase