title: A consolidated bio-processing of ethanol from cassava pulp accompanied by hydrogen production.
abstract: A biphasic fermentation approach was undertaken for the production of ethanol and hydrogen from cassava pulp. The glucose generated by co-culture of Clostridium thermocellum and Thermoanaerobacterium aotearoense was 13.65±0.45 g L(-1), which was 1.75 and 1.17-fold greater than that produced by mono-cultures of C. thermocellum and T. aotearoense, respectively. The accumulated glucose could be utilised rapidly by subsequently inoculated Saccharomyces cerevisiae. An inoculum ratio of 1:1, a thermophilic fermentation of 84 h, and a pulp concentration of 4% proved optimal for ethanol production, fermentation efficiency, and productivity. With these conditions, the ethanol level reached 8.83±0.31 g L(-1) with a fermentation efficiency of 64.95±2.71%. Hydrogen production of 4.06 mmol by the co-culture system was 1.54 and 2.09-fold greater than that produced by mono-cultures of C. thermocellum and T. aotearoense, respectively. This sequential co-culture approach provided a consolidated bio-processing means to produce ethanol and hydrogen from cassava pulp.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Panyu, Guangzhou 510006, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Mingjun
authors.initials: M
authors.lastname: Zhu
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D048013:Acetivibrio thermocellus / Q000378:metabolism; D018920:Coculture Techniques; D000431:Ethanol / Q000138:chemical synthesis*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D002365:Manihot / Q000737:chemistry*; D046950:Thermoanaerobacterium / Q000378:metabolism; D013997:Time Factors; D014866:Waste Products / Q000032:analysis*
chemical_list: D014866:Waste Products; D002245:Carbon Dioxide; D000431:Ethanol; D006859:Hydrogen