title: HPLC methods for determination of D-aspartate and N-methyl-D-aspartate.
abstract: D-Amino acids are stereoisomers or optical isomers of naturally occurring L-amino acids and thus possess the same chemical structure, but may differ in their biological/physiological properties. Until a half century ago, D-amino acids had been considered to be unnatural substances found only in microorganisms. However, improvements in analytical instruments and methods have revealed that D-amino acids are present in invertebrates and vertebrates, including humans, and that they possess important physiological functions. D-Aspartate (D-Asp) and its methylated form N-methyl-D-aspartate (NMDA) possess neuroendocrine properties in many species. Several methods have been developed for determination of D- and L-enantiomers of amino acids by high performance liquid chromatography (HPLC). We report here improved HPLC methods for the specific determination of D-Asp and NMDA in biological tissues.
authors.affiliation: Department of Chemistry, Barry University, Miami Shores, FL, USA. gfisher@mail.barry.edu
authors.forename: George H
authors.initials: GH
authors.lastname: Fisher
authors.forename: Mara
authors.initials: M
authors.lastname: Tsesarskaia
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D026603:D-Aspartic Acid / Q000032:analysis*; D016202:N-Methylaspartate / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D026603:D-Aspartic Acid; D016202:N-Methylaspartate