title: [Expression and significance of IL-10 in human chorionic villi of recurrent spontaneous abortion].
abstract: OBJECTIVE
  To investigate the correlation between interleukin-10(IL-10) and recurrent spontaneous abortion as well as the significance of them through researching the comparison of histological localization of IL-10,  expression comparison of IL-10 mRNA in human chorionic villi of pregnant women with recurrent spontaneous abortion and normal abortion.
  
  
  METHODS
  To detect expression comparison of IL-10 mRNA and histological localization of IL-10 in human chorionic villi of 35 pregnant women with recurrent spontaneous abortion(experimental group) and 35 normal abortion women(control group) by reverse transcription-polymerase chain reaction and immunohistochemistry. To analysis expression of IL-10 in serum of the two groups by ELISA.
  
  
  RESULTS
  The detection of immunohistochemistry showed that there are expressions of IL-10 both in the two groups,  the expressions of IL-10 in the experimental group were lower than the control group(P<0.05).The results of the RT-PCR showed that the expressions of IL-10 of experimental group were lower than the control group(P<0.05). ELISA analysis showed that the expressions of IL-10 of the experimental group were lower than the control group(P<0.05).
  
  
  CONCLUSIONS
  The results of detection of immunohistochemistry and the expression of mRNA of IL-10 in human chorionic villi as well as its expressions in serum of experimental group were lower than the control group. IL-10 would play an important role in maintaining normal pregnancy.
authors.affiliation: Xi'an Medical University, Obstetrics and Gynecology Nursing Staff Room, Xi'an, China. lijing071015@126.com
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yong-ai
authors.initials: YA
authors.lastname: Zhang
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Chu
authors.forename: Li-qin
authors.initials: LQ
authors.lastname: Wang
mesh_terms: D000026:Abortion, Habitual / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D002824:Chorionic Villi / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10