title: Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus                      subtilis chitinase gene chi113.
abstract: Burkholderia                         vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N(2)-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P < 0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type.
authors.affiliation: Shandong Provincial Key Laboratory of Applied Microbiology, Biotechnology Center of Shandong Academy of Sciences, Jinan, 250014, China. zhxjemail@gmail.com
authors.forename: Xinjian
authors.initials: X
authors.lastname: Zhang
authors.forename: Yujie
authors.initials: Y
authors.lastname: Huang
authors.forename: Paul R
authors.initials: PR
authors.lastname: Harvey
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Zhang
authors.forename: Hongzi
authors.initials: H
authors.lastname: Zhou
authors.forename: Hetong
authors.initials: H
authors.lastname: Yang
mesh_terms: D000898:Antibiosis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005658:Fungi / Q000254:growth & development; D015870:Gene Expression; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation; D030781:Organisms, Genetically Modified; D010710:Phosphates / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D011188:Potassium / Q000378:metabolism
chemical_list: D004251:DNA Transposable Elements; D010710:Phosphates; D002688:Chitinases; D011188:Potassium