title: Immunohistochemical analysis of the proteolytic enzyme subtilase in the digestive organs of the sea urchin Strongylocentrotus intermedius.
abstract: Subtilase, a major protease in the short-spined sea urchin (Strongylocentrotus intermedius), was isolated and used as antigen for the subsequent production of a specific polyclonal antibody. Immunoreactive cells were observed by immunohistochemical analysis in granules in the anterior and posterior stomach and the anterior intestine. These granules, which were most numerous in the anterior stomach, also stained intensely with methylene blue-Azure II. However, granules in cells of the esophagus, posterior intestine, and rectum were not stained by this antibody. We conclude that subtilase mainly localizes in the stomach and anterior intestine of the sea urchin.
authors.affiliation: Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University, Hakodate, Japan. kazu@fish.hokudai.ac.jp
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ura
authors.forename: Syuto
authors.initials: S
authors.lastname: Hasegawa
authors.forename: Eri
authors.initials: E
authors.lastname: Tanaka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Gotoh
authors.forename: Takao
authors.initials: T
authors.lastname: Ojima
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Takagi
mesh_terms: D000818:Animals; D004064:Digestive System / Q000033:anatomy & histology / Q000201:enzymology*; D007150:Immunohistochemistry / Q000379:methods; D007422:Intestines / Q000033:anatomy & histology / Q000201:enzymology; D057057:Serine Proteases / Q000737:chemistry / Q000502:physiology*; D013270:Stomach / Q000033:anatomy & histology / Q000201:enzymology; D047269:Strongylocentrotus / Q000033:anatomy & histology / Q000201:enzymology*; D013381:Subtilisins / Q000737:chemistry* / Q000276:immunology / Q000502:physiology*
chemical_list: D057057:Serine Proteases; D013381:Subtilisins