title: Preclinical pharmacokinetics and biodistribution of human papillomavirus DNA vaccine delivered in human endogenous retrovirus envelope-coated baculovirus vector.
abstract: OBJECTIVE
  Test pharmacokinetics and biodistribution of a human papillomavirus(HPV)16L1 DNA vaccine delivered in human endogenous retrovirus envelope protein (HERV)-expressing baculovirus (AcHERV) and those of naked plasmid vaccine.
  
  
  METHODS
  HPV16L1 gene was administrated as a naked plasmid or in AcHERV to mice via intravenous and intramuscular routes. HPV16L1 gene was extracted and assayed by quantitative real-time polymerase chain reaction, which was determined to have a detection limit of 50 copies/µg genomic DNA..
  
  
  RESULTS
  Mean residence times of HPV16L1 in AcHERV were 4.8- and 272.2-fold higher than naked HPV16L1 DNA vaccines after intramuscular and intravenous administration, respectively. Naked HPV16L1 DNA levels 1 month after injection were >3 orders of magnitude lower in each tissue tested than AcHERV-delivered HPV16L1, which was retained in most tissues without specific tissue tropism. AcHERV-delivered HPV16L1 administered intramuscularly persisted at the injection sites. However, the levels of copy numbers in muscle were low (1,800/μg genomic DNA) after 1 month, and undetectable after 6 months.
  
  
  CONCLUSIONS
  HPV16L1 delivered via AcHERV resides longer in the body than HPV16L1 in naked form. The lack of tissue tropism ensures the safety of AcHERV vectors for further development.
authors.affiliation: College of Pharmacy & Research Institute of Pharmaceutical Sciences, Seoul National University, Daehak-dong, Gwanank-gu, Seoul, 151-742, South Korea.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Soondong
authors.initials: S
authors.lastname: Lee
authors.forename: Saewon
authors.initials: S
authors.lastname: Im
authors.forename: Mi-Gyeong
authors.initials: MG
authors.lastname: Kim
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Lee
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Keyong Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Sujeong
authors.initials: S
authors.lastname: Kim
authors.forename: Young Bong
authors.initials: YB
authors.lastname: Kim
authors.forename: Yu-Kyoung
authors.initials: YK
authors.lastname: Oh
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000517:prevention & control*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000493:pharmacokinetics*
chemical_list: D053918:Papillomavirus Vaccines; D019444:Vaccines, DNA