title: Biogenic hydrogen and methane production from Chlorella vulgaris and Dunaliella tertiolecta biomass.
abstract: BACKGROUND
  Microalgae are a promising feedstock for biofuel and bioenergy production due to their high photosynthetic efficiencies, high growth rates and no need for external organic carbon supply. In this study, utilization of Chlorella vulgaris (a fresh water microalga) and Dunaliella tertiolecta (a marine microalga) biomass was tested as a feedstock for anaerobic H2 and CH4 production.
  
  
  RESULTS
  Anaerobic serum bottle assays were conducted at 37°C with enrichment cultures derived from municipal anaerobic digester sludge. Low levels of H2 were produced by anaerobic enrichment cultures, but H2 was subsequently consumed even in the presence of 2-bromoethanesulfonic acid, an inhibitor of methanogens. Without inoculation, algal biomass still produced H2 due to the activities of satellite bacteria associated with algal cultures. CH4 was produced from both types of biomass with anaerobic enrichments. Polymerase chain reaction-denaturing gradient gel electrophoresis profiling indicated the presence of H2-producing and H2-consuming bacteria in the anaerobic enrichment cultures and the presence of H2-producing bacteria among the satellite bacteria in both sources of algal biomass.
  
  
  CONCLUSIONS
  H2 production by the satellite bacteria was comparable from D. tertiolecta (12.6 ml H2/g volatile solids (VS)) and from C. vulgaris (10.8 ml H2/g VS), whereas CH4 production was significantly higher from C. vulgaris (286 ml/g VS) than from D. tertiolecta (24 ml/g VS). The high salinity of the D. tertiolecta slurry, prohibitive to methanogens, was the probable reason for lower CH4 production.
authors.affiliation: Department of Chemistry and Bioengineering, Tampere University of Technology, PO Box 541, FI-33101 Tampere, Finland. aino-maija.lakaniemi@tut.fi.
authors.forename: Aino-Maija
authors.initials: AM
authors.lastname: Lakaniemi
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hulatt
authors.forename: David N
authors.initials: DN
authors.lastname: Thomas
authors.forename: Olli H
authors.initials: OH
authors.lastname: Tuovinen
authors.forename: Jaakko A
authors.initials: JA
authors.lastname: Puhakka