title: Effect of muscle type, sire breed, and time of weaning on fatty acid composition of finishing steers.
abstract: Thirty-three steer calves were used to determine the effect of sire breed (Angus or Charolais), time of weaning [normal weaned at approximately 210 d of age (NW) or late weaned at approximately 300 d of age (LW)], and muscle type [LM and semitendinosus muscle (STN)] on fatty acid composition. The whole plot consisted of a 2 (sire breed) × 2 (time of weaning) treatment arrangement, and the subplot treatment was muscle type. Body weights were recorded at 28-d intervals to determine animal performance. Muscle biopsies were collected on d 127 and 128 of finishing. All calves were slaughtered on d 138, and carcass data were collected. Angus-sired steers had lighter initial BW (271 vs. 298 kg; P = 0.02), and LW steers were heavier (351 vs. 323 kg; P = 0.03) on d 28, but no other differences in BW were noted. Charolais-sired steers had larger LM area (P = 0.03), reduced yield grades (P = 0.01), less 12th-rib fat (P < 0.01), and less marbling (P < 0.01) than Angus-sired steers. Carcass measures overall indicate Angus-sired steers were fatter. Hot carcass weight was heavier (348 vs. 324 kg; P = 0.04) in LW steers than NW steers. No other differences (P > 0.05) were observed for feedlot performance or carcass characteristics. Total lipids were extracted from muscle biopsies, derivatized to their methyl esters, and analyzed using gas chromatography. The LM had greater SFA (43.94 vs. 35.76%; P < 0.01) and decreased unsaturated fatty acids (UFA; 56.90 vs. 66.19%; P < 0.01) compared with the STN. Percent total MUFA was greater in STN than LM (51.05 vs. 41.98%; P < 0.01). Total SFA, UFA, and MUFA did not differ due to sire breed or time of weaning. Total PUFA differed (P = 0.04) due to a sire breed × time of weaning interaction but did not differ due to muscle type, with greater PUFA in NW Charolais than any other sire breed × time of weaning combination. Observed changes in percent MUFA may be a result of greater Δ(9)-desaturase activity. The calculated desaturase index suggests STN has a greater Δ(9)-desaturase activity than LM, but no differences (P > 0.05) between sire breed or time of weaning were observed. These results indicate that sire breed, time of weaning, and muscle type all affect fatty acid composition in beef. This information provides insight into factors for manipulation of beef fatty acids. More research is needed to identify beef cuts based on fatty acid profile and healthfulness.
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506, USA.
authors.forename: A K
authors.initials: AK
authors.lastname: Sexten
authors.forename: C R
authors.initials: CR
authors.lastname: Krehbiel
authors.forename: J W
authors.initials: JW
authors.lastname: Dillwith
authors.forename: R D
authors.initials: RD
authors.lastname: Madden
authors.forename: C P
authors.initials: CP
authors.lastname: McMurphy
authors.forename: D L
authors.initials: DL
authors.lastname: Lalman
authors.forename: R G
authors.initials: RG
authors.lastname: Mateescu
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D001706:Biopsy / Q000662:veterinary; D001835:Body Weight / Q000502:physiology; D002417:Cattle / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016018:Least-Squares Analysis; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000378:metabolism*; D011897:Random Allocation; D014886:Weaning
chemical_list: D005227:Fatty Acids