title: iTRAQ-based quantitative secretome analysis of Phanerochaete chrysosporium.
abstract: The basidiomycete fungi such as Phanerochaete chrysosporium secrete large amount of hydrolytic and oxidative enzymes and degrade lignocellulosic biomass. The lignin depolymerizing proteins were extensively studied, but cellulose, hemicellulose and pectin hydrolyzing enzymes were poorly explored. In this study P. chrysosporium was grown in cellulose, lignin and mixture of cellulose and lignin, and secretory proteins were quantified by isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics using liquid chromatography tandem mass spectrometry (LC-MS/MS). An iTRAQ quantified 117 enzymes comprising cellulose hydrolyzing endoglucanases, exoglucanases, beta-glucosidases; hemicelluloses hydrolyzing xylanases, acetylxylan esterases, mannosidases, mannanases; pectin-degrading enzymes polygalacturonase, rhamnogalacturonase, arabinose and lignin degrading protein belonging to oxidoreductase family. Under cellulose and cellulose with lignin culture conditions, enzymes such as endoglucanases, exoglucanases, β-glucosidases and cellobiose dehydrogenase were significantly upregulated and iTRAQ data suggested hydrolytic and oxidative cellulose degradation. When lignin was used as a major carbon source, enzymes such as copper radical oxidase, isoamyl oxidase, glutathione S-transferase, thioredoxin peroxidase, quinone oxidoreductase, aryl alcohol oxidase, pyranose 2-oxidase, aldehyde dehydrogenase, and alcohol dehydrogenase were expressed and significantly regulated. This study explored cellulose, hemicellulose, pectin and lignin degrading enzymes of P. chrysosporium that are valuable for lignocellulosic bioenergy.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551 Singapore.
authors.forename: Arulmani
authors.initials: A
authors.lastname: Manavalan
authors.forename: Sunil S
authors.initials: SS
authors.lastname: Adav
authors.forename: Siu Kwan
authors.initials: SK
authors.lastname: Sze
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D002853:Chromatography, Liquid; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000235:genetics; D008031:Lignin / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology* / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D020543:Proteome; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D002480:Cellulase