title: Modeling growth, lipid accumulation and lipid turnover in submerged batch cultures of Umbelopsis isabellina.
abstract: The production of lipids by oleaginous yeast and fungi becomes more important because these lipids can be used for biodiesel production. To understand the process of lipid production better, we developed a model for growth, lipid production and lipid turnover in submerged batch fermentation. This model describes three subsequent phases: exponential growth when both a C-source and an N-source are available, carbohydrate and lipid production when the N-source is exhausted and turnover of accumulated lipids when the C-source is exhausted. The model was validated with submerged batch cultures of the fungus Umbelopsis isabellina (formerly known as Mortierella isabellina) with two different initial C/N-ratios. Comparison with chemostat cultures with the same strain showed a significant difference in lipid production: in batch cultures, the initial specific lipid production rate was almost four times higher than in chemostat cultures but it decreased exponentially in time, while the maximum specific lipid production rate in chemostat cultures was independent of residence time. This indicates that different mechanisms for lipid production are active in batch and chemostat cultures. The model could also describe data for submerged batch cultures from literature well.
authors.affiliation: Bioprocess Engineering, Wageningen University, Wageningen, The Netherlands.
authors.forename: Petra
authors.initials: P
authors.lastname: Meeuwse
authors.forename: Payman
authors.initials: P
authors.lastname: Akbari
authors.forename: Johannes
authors.initials: J
authors.lastname: Tramper
authors.forename: Arjen
authors.initials: A
authors.lastname: Rinzema
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods; D019149:Bioreactors / Q000382:microbiology*; D049109:Cell Proliferation; D003198:Computer Simulation; D050356:Lipid Metabolism / Q000502:physiology*; D008055:Lipids / Q000096:biosynthesis*; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D009090:Mucorales / Q000502:physiology*
chemical_list: D008055:Lipids