title: [Identification of proteins overexpressed in malignant gastric tumors: comparison of the results of 2-De and bioinformatics search].
abstract: Comparison of protein expression in intestinal and diffuse stomach tumors by 2D gel electrophoresis led to identification of three proteins (SOD2, S100A6, and TXN), which are overexpressed in tumors as compared to normal controls. It was shown, that overexpression of proteins SOD2 and TXN occurs much more frequently in diffuse tumors than in intestinal ones. A control panel of eleven proteins overexpressed in stomach tumors has been selected based on the data of comparative 2D analysis described in the literature. Bioinformatics search for mRNAs encoding proteins from the control panel in Oncomine database (which contains the results of determination of mRNA transcription level in tumor vs. normal samples) demonstrated the coincidence of proteomic and transcriptomic data for seven out of 11 proteins.
authors.forename: E S
authors.initials: ES
authors.lastname: Grigor'eva
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Bukurova
authors.forename: G S
authors.initials: GS
authors.lastname: Krasnov
authors.forename: S G
authors.initials: SG
authors.lastname: Afanas'ev
authors.forename: N V
authors.initials: NV
authors.lastname: Cherdyntseva
authors.forename: S A
authors.initials: SA
authors.lastname: Tuzikov
authors.forename: E L
authors.initials: EL
authors.lastname: Choĭnzonov
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
authors.forename: N A
authors.initials: NA
authors.lastname: Lisitsyn
authors.forename: S F
authors.initials: SF
authors.lastname: Beresten'
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D040901:Proteomics; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D013879:Thioredoxins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D000099156:Superoxide Dismutase 2
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D009363:Neoplasm Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D013482:Superoxide Dismutase; D013879:Thioredoxins; D000099156:Superoxide Dismutase 2; C491203:TXN protein, human; C055351:S100A6 protein, human