title: An improved method for whole protein extraction from yeast Saccharomyces cerevisiae.
abstract: A new method for protein extraction from yeast Saccharomyces cerevisiae cells is described. This method involves the use of LiAc and NaOH to enhance the permeability of yeast cell wall prior to protein extraction with SDS-PAGE sample buffer. It was safe and efficient compared to other methods reported so far in the literature. The proteins extracted with this new method retained their immunoreactive properties and are suitable for most applications in molecular biology studies.
authors.affiliation: College of Animal Science and Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, YangLing, Shaan'xi, 712100, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Lei
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D029701:Saccharomyces cerevisiae Proteins