title: Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts.
abstract: As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1-3, 3-5, and 5-10 kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3-5 kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3-5 kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3-5 kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.
authors.affiliation: Department of Chemistry, Pukyoung National University, Busan, 608-737, Republic of Korea.
authors.forename: Byul-Nim
authors.initials: BN
authors.lastname: Ahn
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: S W A
authors.initials: SW
authors.lastname: Himaya
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Bak
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives*; D003094:Collagen / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology; D014466:Ultraviolet Rays
chemical_list: D009844:Oligosaccharides; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D003094:Collagen; D048271:Chitosan; D007770:L-Lactate Dehydrogenase; D020928:Mitogen-Activated Protein Kinases; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1