title: Species-dependent sensitivity to contaminants: an approach using primary hepatocyte cultures with three marine fish species.
abstract: There is limited knowledge about the sensitivity of different fish species to environmental pollutants. Such information is pivotal in risk assessment and to understand why some species appear to be more tolerant to contaminants than others. The aim of the current study was to evaluate whether primary hepatocyte cultures of three marine fish species could be established in the field and whether their sensitivity to selected contaminants would differ. Primary hepatocyte cultures of three marine fish species (plaice, long rough dab, Atlantic cod) were established and exposed for 24 h to copper (20-2500 mg L⁻¹) and statins (1-200 mg L⁻¹). Endpoints were esterase activity, metabolic activity and reduced glutathione (GSH) content, all using fluorescent probes. Flatfish hepatocytes were more susceptible to copper and statin exposure than hepatocytes from cod. This study has shown that species-dependent differences in contaminant sensitivity can be investigated using primary hepatocyte cultures.
authors.affiliation: Department of Biology, University of Oslo, P.O. Box 1066 Blindern, N-0316 Oslo, Norway.
authors.forename: Kathrin Sabine
authors.initials: KS
authors.lastname: Ellesat
authors.forename: Mazyar
authors.initials: M
authors.lastname: Yazdani
authors.forename: Tor Fredrik
authors.initials: TF
authors.lastname: Holth
authors.forename: Ketil
authors.initials: K
authors.lastname: Hylland
mesh_terms: D000818:Animals; D000069059:Atorvastatin; D002478:Cells, Cultured; D003300:Copper / Q000737:chemistry / Q000633:toxicity*; D005260:Female; D005399:Fishes / Q000235:genetics; D022781:Hepatocytes / Q000187:drug effects*; D006538:Heptanoic Acids / Q000737:chemistry / Q000633:toxicity*; D008297:Male; D011758:Pyrroles / Q000737:chemistry / Q000633:toxicity*; D019821:Simvastatin / Q000737:chemistry / Q000633:toxicity*; D013045:Species Specificity; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D006538:Heptanoic Acids; D011758:Pyrroles; D014874:Water Pollutants, Chemical; D003300:Copper; D000069059:Atorvastatin; D019821:Simvastatin