title: The gene expression profile of phosphoantigen-specific human γδ T lymphocytes is a blend of αβ T-cell and NK-cell signatures.
abstract: Global transcriptional technologies have revolutionised the study of lymphoid cell populations, but human γδ T lymphocytes specific for phosphoantigens remain far less deeply characterised by these methods despite the great therapeutic potential of these cells. Here we analyse the transcriptome of circulating TCRVγ(+)  γδ T cells isolated from healthy individuals, and their relation with those from other lymphoid cell subsets. We report that the gene signature of phosphoantigen-specific TCRVγ(+)  γδ T cells is a hybrid of those from αβ T and NK cells, with more 'NK-cell' genes than αβ T cells have and more 'T-cell' genes than NK cells. The expression profile of TCRVγ(+)  γδ T cells stimulated with phosphoantigen recapitulates their immediate physiological functions: Th1 cytokine, chemokine and cytotoxic activities reflect their high mitotic activity at later time points and do not indicate antigen-presenting functions. Finally, such hallmarks make the transcriptome of γδ T cells, whether resting or clonally expanding, clearly distinctive from that of NK/T or peripheral T-cell lymphomas of the γδ subtype.
authors.affiliation: INSERM UMR1037, Cancer Research Center of Toulouse, Toulouse, France.
authors.forename: Fréderic
authors.initials: F
authors.lastname: Pont
authors.forename: Julien
authors.initials: J
authors.lastname: Familiades
authors.forename: Sébastien
authors.initials: S
authors.lastname: Déjean
authors.forename: Séverine
authors.initials: S
authors.lastname: Fruchon
authors.forename: Delphine
authors.initials: D
authors.lastname: Cendron
authors.forename: Mary
authors.initials: M
authors.lastname: Poupot
authors.forename: Rémy
authors.initials: R
authors.lastname: Poupot
authors.forename: Fatima
authors.initials: F
authors.lastname: L'faqihi-Olive
authors.forename: Nais
authors.initials: N
authors.lastname: Prade
authors.forename: Bernard
authors.initials: B
authors.lastname: Ycart
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Fournié
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D057225:Data Mining; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology*; D020411:Oligonucleotide Array Sequence Analysis; D010750:Phosphoproteins / Q000276:immunology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D010750:Phosphoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012313:RNA