title: TWIST expression in hypopharyngeal cancer and the mechanism of TWIST-induced             promotion of metastasis.
abstract: The transcription factor TWIST is an important factor in regulating epithelial-mesenchymal             transition (EMT) and tumor metastasis. To explore the functions of TWIST in hypopharyngeal             cancer, we investigated if overexpression of TWIST has an effect on FaDu cell             morphology, and if alteration of TWIST has an effect on E-cadherin, N-cadherin,             c-fos, MMP-9, as well as in cell migration, and the invasion ability of FaDu cells.             Moreover, we also studied the relationship between TWIST overexpression and clinicopathological             characteristics in hypopharyngeal cancer tissue samples by immunohistochemical             assays. The results showed that overexpression of TWIST-induced morphological             changes, such as occurrence of EMT. TWIST overexpression also increased cell migration             and invasion ability, accompanied by an alteration of E-cadherin, N-cadherin,             c-fos and MMP-9 expression. Furthermore, immunohistochemical assays showed that             TWIST overexpression was related with tumor differentiation (P=0.038), tumor size             (P=0.048) and lymph node metastasis (P=0.044). The data presented reveal that             overexpression of TWIST plays a significant role in the metastasis of hypopharyngeal             tumors, and alteration of TWIST has an effect on the EMT, c-fos and MMP-9 expression             in FaDu cells. We conclude that TWIST promotes hypopharyngeal carcinoma metastasis,             and the TWIST/c-fos/MMP-9 signaling pathway may play an important role in the             metastasis of FaDu cells.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Sumei
authors.initials: S
authors.lastname: Lu
authors.forename: Jiajun
authors.initials: J
authors.lastname: Tian
authors.forename: Juke
authors.initials: J
authors.lastname: Ma
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D015820:Cadherins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D016762:Genes, fos; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D035683:MicroRNAs / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D014162:Transfection; D047368:Tumor Burden; D051879:Twist-Related Protein 1 / Q000235:genetics*
chemical_list: D015820:Cadherins; D035683:MicroRNAs; D051879:Twist-Related Protein 1; D020780:Matrix Metalloproteinase 9