title: MtDNA variability in whitefly  (Bemisia tabaci) populations in Brazil.
abstract: Bemisia  tabaci (Hemiptera: Aleyrodidae) consists of a complex of  morphologically indistinct biotypes that vary mainly in their capacity to  transmit plant viruses and to induce physiological disorders in plants of  economic importance. The adaptability of B. tabaci to many regions of  the world has fostered the appearance of various biotypes and has resulted in a  broad spectrum of host plants. Our goal was to identify which biotypes were  present in four B. tabaci populations in Brazil. We quantified genetic  variability between and within populations. Three individuals were collected  from three host plant species: two populations on soybean (Campinas and  Rondonópolis), one on pumpkin (Barreiras) and one on tomato (Cruz das Almas) in  three States of Brazil (São Paulo, Mato Grosso, and Bahia). We chose one  sequence of the B biotype, obtained from GenBank; the Campinas population,  which had been previously characterized as biotype B, was used as a control for  this biotype. We also included one sequence of the Q biotype, obtained from  GenBank, as an outgroup. The COI region of the mtDNA gene was partially  amplified with the CI-J-2195 and L2-N-3014 pair of primers, and the reaction  products were sequenced. Based on distance-based algorithm analyses, we found  that all haplotypes belong to biotype B, which was confirmed by the haplotype  network. Genetic structure analyses showed that the host plant species does not  influence population structuring of this pest; only the geographic location  mattered.
authors.affiliation: Centro de Fitossanidade, Instituto Agrônomico de Campinas, Campinas, SP, Brasil. gevalle@yahoo.com.br
authors.forename: G E
authors.initials: GE
authors.lastname: Valle
authors.forename: A L
authors.initials: AL
authors.lastname: Lourenção
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.forename: A G
authors.initials: AG
authors.lastname: Abreu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D051456:Gene Flow; D017344:Genes, Insect; D005819:Genetic Markers; D014644:Genetic Variation; D006430:Hemiptera / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D005819:Genetic Markers