title: Cyclophilin A-EMMPRIN interaction induces invasion of head and neck             squamous cell carcinoma.
abstract: Extracellular matrix remodeling crucial to tumorigenesis involves proteolytic             enzymes, primarily matrix metalloproteinases (MMPs). MMP production is stimulated             by multiple factors, including the extracellular matrix metallo-proteinase inducer             EMMPRIN/CD147. Overexpression of EMMPRIN, a member of the immunoglobulin superfamily,             promotes invasion, metastasis, growth and survival of malignant cells. Cyclophilin             A (CypA) is a multifunctional protein that promotes cancer progression in various             cancer types. CypA can interact with and activate EMMPRIN; however, the role of             CypA-EMMPRIN interaction in oncogenicity is not completely understood. To investigate             tumorigenicity induced by the CypA-EMMPRIN interaction, we stimulated EMMPRIN-expressing             head and neck squamous cell carcinoma (HNSCC) cells with CypA. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium             bromide dye assay revealed that HNSCC cell proliferation increased upon stimulation             of the cells with CypA, whereas cisplatin-induced cell death decreased after stimulation.             Gelatin zymography showed that CypA also induced MMP-9 up-regulation. Moreover,             HNSCC cell invasion through Matrigel™-coated membranes was increased upon stimulation             of cells with CypA. This elevated invasive potential was abrogated by an EMMPRIN             function-blocking antibody. These findings suggest that CypA, through its interaction             with EMMPRIN, contributes to HNSCC tumorigenesis.
authors.affiliation: Department of Otorhinolaryngology and Head and Neck Surgery,                     Akita University Graduate School of Medicine, Akita 010-8543, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Suzuki
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ishikawa
mesh_terms: D051926:Basigin / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D021984:Cyclophilin A / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015151:Immunoblotting; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness
chemical_list: C497886:BSG protein, human; D051926:Basigin; D020780:Matrix Metalloproteinase 9; D021984:Cyclophilin A