title: Dynamic instability of central airways and peripheral airspace in rat lungs perfused with cold preservation solutions.
abstract: OBJECTIVE
  For lung preservation, one of two types of solutions is commonly employed: Euro-Collins (EC) or low potassium dextran glucose (LPDG). These two solutions have been compared regarding biological, morphometrical and physiological outcomes in many experiments. However, the dynamic mechanics of perfused lung are not well understood because the dynamic characteristics cannot be assessed under static conditions; hence, the primary goal of the present study was to assess this in perfused rat lungs during the preservation period, comparing EC with LPDG at 0 or 9 h at 4°C.
  
  
  METHODS
  Lung impedance was measured using a forced oscillation technique. Lung resistance and elastance values were obtained by the fast Fourier transform algorithm. The instability of central airways and heterogeneity of ventilation were estimated.
  
  
  RESULTS
  In the EC group, airway resistance and instability were high after perfusion, and the lung elastance was high and more heterogeneous after cold storage. In contrast, those parameters were stable in the LPDG group during cold storage.
  
  
  CONCLUSIONS
  Such dynamic stability might facilitate the handling of lung grafts and eliminate injurious cyclic ventilation stress after reperfusion. Thus, we conclude that the impedance frequency characteristic represents a novel informative parameter for investigating lung preservation techniques.
authors.affiliation: Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hamakawa
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Aoyama
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Fujinaga
authors.forename: H
authors.initials: H
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Date
authors.forename: T
authors.initials: T
authors.lastname: Bando
mesh_terms: D000403:Airway Resistance; D000818:Animals; D003080:Cold Temperature; D003911:Dextrans; D005947:Glucose; D006982:Hypertonic Solutions; D008168:Lung / Q000503:physiopathology*; D016040:Lung Transplantation / Q000502:physiology; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D051381:Rats; D017208:Rats, Wistar; D015656:Respiratory Mechanics
chemical_list: D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; C083290:low potassium dextran glucose solution; D005947:Glucose