title: The alteration of components in the fermented Hwangryunhaedok-tang and its neuroprotective activity.
abstract: BACKGROUND
  Hwangryunhaedok-tang is a traditional herbal prescription that has sedative activity, hypotensive and anti-bacterial effects.
  
  
  OBJECTIVE
  In this study, we investigated the alteration of contents of components in Hwangryunhaedok-tang, antioxidant activity and neuroprotective activity by fermentation with Lactobacillus acidophilus KFRI 128.
  
  
  METHODS
  Contents of three marker compounds (geniposide, berberine and palmatine) and unknown compounds in the Hwangryunhaedok-tang (HR) and the fermented Hwangryunhaedok-tang (FHR) were measured and compared using the established high-performance liqued chromatograph coupled with a photodiode (HPLC-DAD) method. The antioxidant activity of HR and FHR were determined by DPPH free radical and hydrogen peroxide (H(2)O(2)) scavenging assay. Also, the neuroprotective activities of HR and FHR against glutamate-induced oxidative stress in a mouse hippocampal cell line (HT22) were evaluated by MTT assay.
  
  
  RESULTS
  The contents of geniposide and palmatine were decreased but the content of berberine was increased in the FHR. And the contents of unknown compounds (1), (2), (3), (4) and (5) in the HR were altered by fermentation. Electron donating activity (EDA, %) value of FHR was higher than HR for DPPH radical scavenging activity and H2O2 scavenging activity, respectively. In the MTT assay, FHR showed more potent neuroprotective activity than HR by 513.90%.
  
  
  CONCLUSIONS
  The FHR using microorganism could convert compounds in HR and enhance the antioxidant and neuroprotective activity.
authors.affiliation: Department of Biomaterials Engineering, Division of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yang
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma