title: Interaction of human serum album and C₆₀ aggregates in solution.
abstract: An important property of C(60) in aquatic ecotoxicology is that it can form stable aggregates with nanoscale dimensions, namely nC(60). Aggregation allows fullerenes to remain suspended for a long time, and the reactivity of individual C(60) is substantially altered in this aggregate form. Herein, we investigated the interaction of nC(60) and human serum album (HSA) using the methods of fluorescence, fluorescence dynamics, circular dichroism (CD), and site marker competitive experiments. We proposed a binding model consistent with the available experimental results for the interactions of nC(60) with HSA. During the interaction process, the structure and conformation of HSA were affected, leading to functional changes of drug binding sites of HSA.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China; E-Mails:  smsong@rcees.ac.cn  (M.S.);  liushufang405@163.com  (S.L.);  jfyin@rcees.ac.cn  (J.Y.).
authors.forename: Maoyong
authors.initials: M
authors.lastname: Song
authors.forename: Shufang
authors.initials: S
authors.lastname: Liu
authors.forename: Junfa
authors.initials: J
authors.lastname: Yin
authors.forename: Hailin
authors.initials: H
authors.lastname: Wang
mesh_terms: D002942:Circular Dichroism; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D037741:Fullerenes; D012709:Serum Albumin; D012996:Solutions; C069837:fullerene C60