title: Measurement of carbonic anhydrase isozyme VI (CA-VI) in swine sera, colostrums, saliva, bile, seminal plasma and tissues.
abstract: Swine secretory carbonic anhydrase VI (CA-VI) was purified from swine saliva and an antibody to CA-VI was generated. A specific and sensitive enzyme-linked immunosorbent assay (ELISA) has been developed for the measurement of swine CA-VI. The assay can detect as little as 5 ng/mL of swine CA-VI. Typical standard curves were determined for a range of CA-VI solutions (7.8 to 500 ng/mL). The coefficients of variation for these solutions were less than 5%. When 500, 250 or 100 ng/mL of swine CA-VI was added to swine sera, the recoveries were 102.0%, 109.7% and 100.2%, respectively. The concentrations of CA-VI in the saliva (26.2 ± 30.4 µg/mL), sera (3.3 ± 4.9 ng/mL), bile (153.0 ± 114.0 ng/mL), seminal plasma (124.0 ± 39.0 ng/mL) and parotid gland (441.3 ± 90.0 µg/g wet tissue), submaxillary gland (88.1 ± 124.4 µg/g wet tissue), sublingual gland (58.6 ± 24.6 µg/g wet tissue) and gallbladder (2.4 ± 1.3 µg/1g wet tissue) were determined by ELISA. The concentration of CA-VI in colostrum was 163.3 ± 101.4 ng/mL and did not decrease within 10 days following parturition. An immunohistochemical reaction to anti-CA-VI antiserum was observed in the columnar epithelial cells lining the gallbladder. These data suggest that secretory CA-VI plays various roles in pH regulation and the maintenance of ion and fluid balance.
authors.affiliation: School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan. nishida@azabu-u.ac.jp
authors.forename: Toshiho
authors.initials: T
authors.lastname: Nishita
authors.forename: Seigo
authors.initials: S
authors.lastname: Itoh
authors.forename: Sachiko
authors.initials: S
authors.lastname: Arai
authors.forename: Nobutune
authors.initials: N
authors.lastname: Ichihara
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Arishima
mesh_terms: D000818:Animals; D001646:Bile / Q000201:enzymology*; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification / Q000502:physiology; D003126:Colostrum / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification / Q000502:physiology; D011247:Pregnancy; D012463:Saliva / Q000201:enzymology*; D012661:Semen / Q000201:enzymology*; D013552:Swine / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D002256:Carbonic Anhydrases; C477051:carbonic anhydrase VI