title: The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation.
abstract: RNA polymerase (pol) II establishes many protein-protein interactions with transcriptional regulators to coordinate different steps of transcription. Although some of these interactions have been well described, little is known about the existence of RNA pol II regions involved in contact with transcriptional regulators. We hypothesize that conserved regions on the surface of RNA pol II contact transcriptional regulators. We identified such an RNA pol II conserved region that includes the majority of the "foot" domain and identified interactions of this region with Mvp1, a protein required for sorting proteins to the vacuole, and Spo14, a phospholipase D. Deletion of MVP1 and SPO14 affects the transcription of their target genes and increases phosphorylation of Ser5 in the carboxy-terminal domain (CTD). Genetic, phenotypic, and functional analyses point to a role for these proteins in transcriptional initiation and/or early elongation, consistent with their genetic interactions with CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme.
authors.affiliation: Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071 Jaén, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: García-López
authors.forename: Vicent
authors.initials: V
authors.lastname: Pelechano
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Mirón-García
authors.forename: Ana I
authors.initials: AI
authors.lastname: Garrido-Godino
authors.forename: Alicia
authors.initials: A
authors.lastname: García
authors.forename: Olga
authors.initials: O
authors.lastname: Calvo
authors.forename: Michel
authors.initials: M
authors.lastname: Werner
authors.forename: José E
authors.initials: JE
authors.lastname: Pérez-Ortín
authors.forename: Francisco
authors.initials: F
authors.lastname: Navarro
mesh_terms: D000595:Amino Acid Sequence; D047369:Chromatin Immunoprecipitation; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D012319:RNA Polymerase II