title: Antitussive, expectorant and anti-inflammatory alkaloids from Bulbus Fritillariae Cirrhosae.
abstract: The primary objective of this study is to evaluate the antitussive, expectorant and anti-inflammatory effects of alkaloids imperialine (I), chuanbeinone (II), verticinone (III), and verticine (IV), which were isolated from the Bulbus Fritillariae Cirrhosae (BFC) using phytochemical method. The results showed that all the alkaloids significantly inhibited cough frequency and increased latent period of cough in mice induced by ammonia. Besides, imperialine(I), verticinone(III) and verticine(IV) markedly enhanced mice's tracheal phenol red output in expectorant evaluation, and imperialine(I), chuanbeinone(II) significantly inhibited the development of ear edema in a dose-dependent manner in anti-inflammatory assessment. Moreover, important differences were found among the structure-activity relationships for the four alkaloids. These results confirmed that the four alkaloids imperialine, chuanbeinone, verticinone and verticine may be the active ingredients of the Bulbus F. Cirrhosae (BFC).
authors.affiliation: Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, PR China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.forename: Jingyi
authors.initials: J
authors.lastname: Zhu
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Ou
authors.forename: Dandan
authors.initials: D
authors.lastname: Wei
authors.forename: Xueping
authors.initials: X
authors.lastname: Li
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000641:Ammonia; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000996:Antitussive Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D003371:Cough / Q000139:chemically induced / Q000188:drug therapy*; D004487:Edema / Q000188:drug therapy; D005100:Expectorants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005260:Female; D031410:Fritillaria / Q000737:chemistry*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D018517:Plant Roots; D013329:Structure-Activity Relationship; D014132:Trachea / Q000187:drug effects
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000996:Antitussive Agents; D005100:Expectorants; D010936:Plant Extracts; D000641:Ammonia