title: Effect of probiotic fermented milk and chlorophyllin on gene expressions and genotoxicity during AFB₁-induced hepatocellular carcinoma.
abstract: The aim of this study was to investigate the chemopreventive effect of probiotic fermented milk and chlorophyllin on aflatoxin B₁ (AFB₁) induced hepatocellular carcinoma. In vivo trials were conducted on 200 Wistar rats allocated to eight groups. Rats in the positive control group were given intraperitoneal injection of aflatoxin B₁ at 450 μg/kg body weight twice a week for 6 weeks. The rats were sacrificed and dissected at 25th week of the experiment, and comet assay was carried out in hepatic cells to assess the genotoxicity or DNA damage. The tumour incidence was decreased by approximately one-third than AFB₁ control group. The expression of c-myc bax, bcl-2, cyclin D1, p53 and rasp-21 genes was also studied. A significant (P<0.05) reduction in DNA damage was observed in probiotic fermented milk with chlorophyllin group as compared to aflatoxin B₁ control group. The c-myc, bcl-2, cyclin D1 and rasp-21 level was found to be highest in AFB₁ control group as compared to the treatment group. The results advocate the enhanced protective potential of probiotic fermented milk and chlorophyllin against AFB₁-induced molecular alterations in hepatic cells during carcinogenesis.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.forename: Vinod
authors.initials: V
authors.lastname: Verma
authors.forename: Ravinder
authors.initials: R
authors.lastname: Nagpal
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Gautam
authors.forename: Pradip V
authors.initials: PV
authors.lastname: Behare
authors.forename: Chand R
authors.initials: CR
authors.lastname: Grover
authors.forename: Praveen K
authors.initials: PK
authors.lastname: Aggarwal
mesh_terms: D016604:Aflatoxin B1; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000517:prevention & control; D002735:Chlorophyllides / Q000494:pharmacology*; D020552:Comet Assay; D043302:Cultured Milk Products; D004249:DNA Damage; D015870:Gene Expression; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000517:prevention & control; D008297:Male; D009153:Mutagens; D019936:Probiotics / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002735:Chlorophyllides; D009153:Mutagens; D016604:Aflatoxin B1; C007020:chlorophyllin