title: The ribosomal protein L19 mRNA is induced by copper exposure in the swordtail fish, Xiphophorus helleri.
abstract: Teleosts are useful vertebrate model species for understanding copper toxicity due to the dual entry route for copper intake via the gills and intestine. In this present study, we utilized the differential display reverse transcription-polymerase chain reaction to isolate potential novel hepatic genes induced by sublethal copper exposure in the freshwater swordtail fish, Xiphophorus helleri. Full length cloning of a cDNA fragment induced by copper exposure to 1 μg/ml during 24 h resulted in the positive identification of a hepatic ribosomal protein L19 (RPL19) gene. Further characterization of this gene revealed that its transcriptional expression was dependent on dosage and time of copper exposure. This study describes for the first time the involvement of RPL19 in copper toxicity, probably as a result of increase in ribosome synthesis rate to support activities such as cellular protein translation, transcriptional activation and mRNA stabilization during sublethal copper exposure.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Minden, Malaysia.
authors.forename: Dwinna
authors.initials: D
authors.lastname: Aliza
authors.forename: Chiew-Lian
authors.initials: CL
authors.lastname: Tey
authors.forename: Ida Shazrina
authors.initials: IS
authors.lastname: Ismail
authors.forename: Meng-Kiat
authors.initials: MK
authors.lastname: Kuah
authors.forename: Alexander Chong
authors.initials: AC
authors.lastname: Shu-Chien
authors.forename: Tengku Sifzizul Tengku
authors.initials: TS
authors.lastname: Muhammad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003300:Copper / Q000633:toxicity*; D003532:Cyprinodontiformes / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004781:Environmental Exposure; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D003300:Copper