title: Immunohistochemical characterisation of dorsal root ganglia neurons supplying the porcine mammary gland.
abstract: The present study investigated the chemical coding of mammary gland-projecting dorsal root ganglia (DRG) neurons using double-labelling immunohistochemistry. Earlier investigations revealed the presence of Fast blue - positive (FB+) neurons in Th9-Th12 DRG after injection of the tracer into the second, right thoracic mamma. Neurons projecting to the last right abdominal mamma were found in L1-L3 DRG. In the present study, the cryostat sections from these ganglia were stained for calcitonin gene-related peptide (CGRP), substance P (SP), nitric oxide synthase (NOS), galanin (GAL) and pituitary adenylate cyclase activating polypeptide (PACAP). Immunohistochemistry revealed that the vast majority of FB+ mammary gland-projecting neurons contained immunoreactivity to CGRP (68.87±0.7%), SP (63.4±0.9%), NOS (32.47±0.9%), GAL (16.28±0.8%) and less numerous nerve cells stained for PACAP (5.87±0.5%). The present results largely correspond with findings dealing with immunohistochemical characterization of nerve fibres supplying porcine mammary gland structures described earlier.
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. ameliaf@uwm.edu.pl
authors.forename: Amelia
authors.initials: A
authors.lastname: Franke-Radowiecka
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005260:Female; D019004:Galanin / Q000032:analysis; D005727:Ganglia, Spinal / Q000737:chemistry* / Q000166:cytology; D007150:Immunohistochemistry; D008321:Mammary Glands, Animal / Q000294:innervation*; D009474:Neurons / Q000737:chemistry*; D019001:Nitric Oxide Synthase / Q000032:analysis; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000032:analysis; D013373:Substance P / Q000032:analysis; D013552:Swine
chemical_list: D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D013373:Substance P; D019004:Galanin; D019001:Nitric Oxide Synthase; D015740:Calcitonin Gene-Related Peptide