title: Isolation and characterization of tetranucleotide repeat polymorphic microsatellite loci in Larus saundersi (Aves, Laridae).
abstract: Nine polymorphic microsatellite loci were isolated, using  tetranucleotide repeat oligonucleotide probes from an enriched DNA library of  the globally "vulnerable" Saunders's gull (Larus saundersi), collected  from the Yancheng coastal wetland, one of the three remaining breeding sites in  China. Six breast muscle tissues and 16 blood samples from 22 gulls and eight  eggshell membrane tissues were collected for this analysis. The number of  alleles per locus ranged from 4 to 15, with a mean of 8.9. The observed and  expected heterozygosities ranged from 0.58 to 0.89 and 0.58 to 0.9, with means  of 0.77 and 0.81, respectively. No significant linkage disequilibrium and no  divergence from Hardy-Weinberg equilibrium were detected among these loci.  Based on Micro-Checker tests, no null alleles are present at any of the loci.  The microsatellite loci described here will be valuable for exploring  population genetic structure and for other relevant genetic studies of  Saunders's gull.
authors.affiliation: Key Laboratory of Forestry Protection of State Forestry Administration, Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China.
authors.forename: H-X
authors.initials: HX
authors.lastname: Jiang
authors.forename: S L
authors.initials: SL
authors.lastname: Ren
authors.forename: Y-Q
authors.initials: YQ
authors.lastname: Hou
authors.forename: M
authors.initials: M
authors.lastname: Yang
authors.forename: X-B
authors.initials: XB
authors.lastname: Wu
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D046091:Charadriiformes / Q000235:genetics*; D017931:DNA Primers; D015723:Gene Library; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers