title: Camelus dromedarius putative cytochrome P450 enzyme CYP2E1: complete coding sequence and phylogenetic tree.
abstract: This study determined the full-length sequence of CYP2E1, one of six cytochrome P450 genes previously examined in camel tissues by western blotting and semi-quantitative PCR. The Camelus dromedarius CYP2E1 has an open reading frame of 1,473 bp, and the cDNA encodes a protein of 490 amino acid residues with a molecular weight of 54.8 kDa. The deduced amino acid sequence showed the highest identity with Bos taurus (88%), Sus scrofa (87%), and Homo sapiens (83%). In a phylogenetic analysis, the C. dromedarius CYP2E1 isoform was located beside cattle and pigs. The deduced amino acid sequence of camel CYP2E1 showed the conserved proline-rich amino terminus and the heme-binding signature localized near the carboxy terminus of the protein.
authors.affiliation: Biochemistry Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammad Saud
authors.initials: MS
authors.lastname: Alanazi
authors.forename: Hesham Mahmoud
authors.initials: HM
authors.lastname: Saeed
authors.forename: Zainul Ariffen
authors.initials: ZA
authors.lastname: Abduljaleel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002162:Camelus / Q000235:genetics*; D003001:Cloning, Molecular; D019392:Cytochrome P-450 CYP2E1 / Q000737:chemistry / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein
chemical_list: D019392:Cytochrome P-450 CYP2E1