title: Proteomic profiling of human respiratory epithelia by iTRAQ reveals biomarkers of exposure and harm by tobacco smoke components.
abstract: Historically, it has been challenging to go beyond epidemiology to investigate the pathogenic changes caused by tobacco smoking. The EpiAirway-100 (MatTek Corp., Ashland, MA) was employed to investigate the effects of cigarette smoke components. Exposure at the air-liquid-interface represented particle and vapour phase components of cigarette smoke. A proteomic study utilising iTRAQ labelling compared expression profiles. The correlative histopathology revealed focal regions of hyperplasia, hypertrophy, cytolysis and necrosis. We identified 466 proteins, 250 with a parameter of two or more peptides. Four of these proteins are potential markers of lung injury and three are related to mechanistic pathways of disease.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, UK. Sextonk@cardiff.ac.uk
authors.forename: Keith
authors.initials: K
authors.lastname: Sexton
authors.forename: Dominique
authors.initials: D
authors.lastname: Balharry
authors.forename: Paul
authors.initials: P
authors.lastname: Brennan
authors.forename: James
authors.initials: J
authors.lastname: McLaren
authors.forename: Ian A
authors.initials: IA
authors.lastname: Brewis
authors.forename: Kelly A
authors.initials: KA
authors.lastname: BéruBé
mesh_terms: D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D020545:Respiratory Mucosa / Q000378:metabolism* / Q000473:pathology; D012907:Smoking; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D020543:Proteome