title: Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration.
abstract: A bi-layered silicon-releasable membrane consisting of a siloxane-poly(lactic acid) (PLA)-vaterite hybrid material (Si-PVH) microfiber mesh and a PLA microfiber mesh has been developed by an electrospinning method for guided bone regeneration (GBR) application. The bi-layered membrane was modified to a three-laminar structure by sandwiching an additional PLA microfiber mesh between the Si-PVH and PLA microfiber meshes (Si-PVH/PLA membrane). In this study, the influence of gamma irradiation, used for sterilization, on biological properties of the Si-PVH/PLA membrane was evaluated with osteoblasts and fibroblasts. After gamma irradiation, while the average molecular weight of the Si-PVH/PLA membrane decreased, the Si-PVH/PLA membrane promoted cell proliferation and differentiation (alkaline phosphatase activity and calcification) of osteoblasts, compared with the poly(lactide-co-glycolide) membrane. These results suggest that the gamma-irradiated Si-PVH/PLA membrane is biocompatible with both fibroblasts and osteoblasts, and may have an application for GBR.
authors.affiliation: Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.
authors.forename: Naoshi
authors.initials: N
authors.lastname: Takeuchi
authors.forename: Miho
authors.initials: M
authors.lastname: Machigashira
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamashita
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kasuga
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Noguchi
authors.forename: Seiji
authors.initials: S
authors.lastname: Ban
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D001861:Bone Regeneration / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology; D002119:Calcium Carbonate / Q000737:chemistry* / Q000528:radiation effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004867:Equipment Design; D005347:Fibroblasts / Q000502:physiology; D005720:Gamma Rays; D005881:Gingiva / Q000166:cytology; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry* / Q000528:radiation effects; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010006:Osteoblasts / Q000502:physiology; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry / Q000528:radiation effects; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry* / Q000528:radiation effects; D012833:Siloxanes / Q000737:chemistry* / Q000528:radiation effects; D013242:Sterilization / Q000379:methods; D013526:Surgical Mesh; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D012833:Siloxanes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D000469:Alkaline Phosphatase; D002119:Calcium Carbonate