title: Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness.
abstract: A new regulatory pathway involved in plant response to oxidative stress was revealed using the iron-induced Arabidopsis ferritin AtFER1 as a model. Using pharmacological and genetic approaches, the DownSTream (DST) cis-acting element in the 3'-untranslated region of the AtFER1 mRNA was shown to be involved in the degradation of this transcript, and oxidative stress triggers this destabilization. In the two previously identified trans-acting mutants (dst1 and dst2), AtFER1 mRNA stability is indeed impaired. Other iron-regulated genes containing putative DST sequences also displayed altered expression. Further physiological characterization identified this oxidative stress-induced DST-dependent degradation pathway as an essential regulatory mechanism to modulate mRNA accumulation patterns. Alteration of this control dramatically impacts plant oxidative physiology and growth. In conclusion, the DST-dependent mRNA stability control appears to be an essential mechanism that allows plants to cope with adverse environmental conditions.
authors.affiliation: Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Montpellier 2, Montpellier Cedex 1, France.
authors.forename: Karl
authors.initials: K
authors.lastname: Ravet
authors.forename: Guilhem
authors.initials: G
authors.lastname: Reyt
authors.forename: Nicolas
authors.initials: N
authors.lastname: Arnaud
authors.forename: Gabriel
authors.initials: G
authors.lastname: Krouk
authors.forename: El-Batoul
authors.initials: el-B
authors.lastname: Djouani
authors.forename: Jossia
authors.initials: J
authors.lastname: Boucherez
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Briat
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gaymard
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D005293:Ferritins / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism*; D018384:Oxidative Stress; D020871:RNA Stability; D018749:RNA, Plant / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; D005293:Ferritins; D007501:Iron