title: Evaluation of a wound dressing composed of hyaluronic acid and collagen sponge containing epidermal growth factor in diabetic mice.
abstract: This study investigated the effect of a wound dressing composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF) on wound healing in diabetic mice. High-molecular-weight (HMW) HA aqueous solution, hydrolyzed low-molecular-weight (LMW) HA aqueous solution and heat-denatured Col aqueous solution were mixed, followed by freeze-drying to obtain a spongy sheet. Cross-linkage between Col molecules was induced by UV irradiation to the spongy sheet (Type-I wound dressing). In a similar manner, a spongy sheet containing EGF (Type-II wound dressing) was prepared by freeze-drying the mixed solution of HMW-HA, LMW-HA and Col containing EGF. The efficacy of these products was evaluated in type-II diabetic BKS.Cg-+Lepr(db)/+Lepr(db) (db/db) mice. Wound dressings were applied to a full-thickness, dorsal skin defect measuring 1.5 cm × 2.0 cm, showing adipose tissue. In the control group, a commercially available artificial dermis composed of collagen spongy sheet (TERUDERMIS(®)) was used. A commercially available polyurethane film dressing (Bioclusive(®)) was applied over each wound dressing. After 1 week of application, wound conditions were evaluated based on their gross and histological appearances. Type-I and -II wound dressings promoted a decrease in wound size associated with angiogenesis and granulation tissue formation, compared with the artificial dermis. In particular, Type-II wound dressings promoted sufficient re-epithelialization. These findings indicate that the combination of HA, Col and EGF promotes wound healing by stimulating cell activity including cell migration and proliferation on the adipose tissue in a diabetic wound. Type-I and -II wound dressings would be useful to prepare a well-vascularized wound bed acceptable for split-thickness auto-skin grafting.
authors.affiliation: a R&D Center for Artificial Skin, School of Allied Health Sciences, Kitasato University , 1-15-1 Kitasato, Minami-ku , Sagamihara , Kanagawa , 252-0373 , Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kondo
authors.forename: Hayato
authors.initials: H
authors.lastname: Niiyama
authors.forename: Akane
authors.initials: A
authors.lastname: Yu
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Kuroyanagi
mesh_terms: D000818:Animals; D001458:Bandages; D003094:Collagen / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D005612:Freeze Drying; D006820:Hyaluronic Acid / Q000737:chemistry; D008297:Male; D008822:Mice, Transgenic; D008970:Molecular Weight; D011140:Polyurethanes; D020011:Protective Agents / Q000008:administration & dosage*; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000473:pathology / Q000503:physiopathology; D012996:Solutions; D016896:Treatment Outcome; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D011140:Polyurethanes; D020011:Protective Agents; D012996:Solutions; D014867:Water; D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid; D003094:Collagen