title: Single-step fabrication of patterned gold film array by an engineered multi-functional peptide.
abstract: This study constitutes a demonstration of the biological route to controlled nano-fabrication via modular multi-functional inorganic-binding peptides. Specifically, we use gold- and silica-binding peptide sequences, fused into a single molecule via a structural peptide spacer, to assemble pre-synthesized gold nanoparticles on silica surface, as well as to synthesize nanometallic particles in situ on the peptide-patterned regions. The resulting film-like gold nanoparticle arrays with controlled spatial organization are characterized by various microscopy and spectroscopy techniques. The described bio-enabled, single-step synthetic process offers many advantages over conventional approaches for surface modifications, self-assembly and device fabrication due to the peptides' modularity, inherent biocompatibility, material specificity and catalytic activity in aqueous environments. Our results showcase the potential of artificially-derived peptides to play a key role in simplifying the assembly and synthesis of multi-material nano-systems in environmentally benign processes.
authors.affiliation: GEMSEC - Genetically Engineered Materials Science and Engineering Center, Materials Science and Engineering Department, University of Washington, Seattle, WA 98195-2120, USA.
authors.forename: Marketa
authors.initials: M
authors.lastname: Hnilova
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Khatayevich
authors.forename: Alisa
authors.initials: A
authors.lastname: Carlson
authors.forename: Ersin Emre
authors.initials: EE
authors.lastname: Oren
authors.forename: Carolyn
authors.initials: C
authors.lastname: Gresswell
authors.forename: Sam
authors.initials: S
authors.lastname: Zheng
authors.forename: Fumio
authors.initials: F
authors.lastname: Ohuchi
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sarikaya
authors.forename: Candan
authors.initials: C
authors.lastname: Tamerler
mesh_terms: D006046:Gold / Q000737:chemistry*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D015202:Protein Engineering; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D008567:Membranes, Artificial; D010455:Peptides; D006046:Gold; D012822:Silicon Dioxide