title: Mesenchymal stem cell like (MSCl) cells generated from human embryonic stem cells support pluripotent cell growth.
abstract: Mesenchymal stem cell like (MSCl) cells were generated from human embryonic stem cells (hESC) through embryoid body formation, and isolated by adherence to plastic surface. MSCl cell lines could be propagated without changes in morphological or functional characteristics for more than 15 passages. These cells, as well as their fluorescent protein expressing stable derivatives, efficiently supported the growth of undifferentiated human embryonic stem cells as feeder cells. The MSCl cells did not express the embryonic (Oct4, Nanog, ABCG2, PODXL, or SSEA4), or hematopoietic (CD34, CD45, CD14, CD133, HLA-DR) stem cell markers, while were positive for the characteristic cell surface markers of MSCs (CD44, CD73, CD90, CD105). MSCl cells could be differentiated toward osteogenic, chondrogenic or adipogenic directions and exhibited significant inhibition of mitogen-activated lymphocyte proliferation, and thus presented immunosuppressive features. We suggest that cultured MSCl cells can properly model human MSCs and be applied as efficient feeders in hESC cultures.
authors.affiliation: Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Varga
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Veréb
authors.forename: Eva
authors.initials: E
authors.lastname: Rajnavölgyi
authors.forename: Katalin
authors.initials: K
authors.lastname: Német
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
authors.forename: Balázs
authors.initials: B
authors.lastname: Sarkadi
authors.forename: Agota
authors.initials: A
authors.lastname: Apáti
mesh_terms: D050156:Adipogenesis; D015415:Biomarkers / Q000378:metabolism; D001709:Biotechnology; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D020219:Chondrogenesis; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007108:Immune Tolerance; D007165:Immunosuppression Therapy; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D010012:Osteogenesis; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers