title: [Detection of human papillomavirus types 16 and 18 in semen samples from patients in an assisted reproduction program].
abstract: BACKGROUND
  The infection with human papillomavirus (HPV) represents a serious health problem in the world. This is because are associated with cervical cancer development in when HPV of high-risk type 16 and 18 are involved. A genital infection by these virus types are normally asymptomatic in the male and an infection to sexual partner is possible.
  
  
  OBJECTIVE
  The objective of the present is the detection of HPV-16 and HPV-18 in semen samples from patients included in an assisted reproduction protocol.
  
  
  METHODS
  Semen samples were obtained from 149 patients that are included in an assisted reproduction protocol in our institution. Semen was examined according with WHO criteria and detection of HPV-16 and HPV-18 was realized with real time PCR protocol with commercial kits. Also, conventional histology techniques were used to assess spermatozoo morphology and leukocyte count.
  
  
  RESULTS
  149 semen samples were analyzed from patients with an age average 37.27 (22-56 age). The 56.18% present oligozoospermic and take into account all patients, 59.73% was positive for HPV-16 (29.56%), HPV-18 (16.11%) or both (14.09%). The infection with HPV-16 was more frequent in both oligozoospermic and normozoospermic patients. In this latter, a minus abnormal spermatic cells and leukocytes were found in regard to oligozoospermic patients.
  
  
  CONCLUSIONS
  There is an important percentage off human asymptomatic male subjects that present in your semen a positive result for high risk papillomavirus. This is very important for the sexual partner because this represent a problem for public health that most be in attention.
authors.affiliation: Centro Médico Nacional 20 de Noviembre, ISSSTE, México, DF. drignacioflores@yahoo.com.mx
authors.forename: Ignacio
authors.initials: I
authors.lastname: Flores-Sánchez
authors.forename: José
authors.initials: J
authors.lastname: Gutiérrez-Salinas
authors.forename: Evangelina
authors.initials: E
authors.lastname: Enriquez-Alvarado
authors.forename: Sergio
authors.initials: S
authors.lastname: Hernández-Rodríguez
authors.forename: Claudia
authors.initials: C
authors.lastname: Ramos-Barragán
authors.forename: Alejandra
authors.initials: A
authors.lastname: Salamanca-Ceciliano
authors.forename: Leticia
authors.initials: L
authors.lastname: Cruz-Tovar
authors.forename: Sigrit
authors.initials: S
authors.lastname: Suástegui-Domínguez
mesh_terms: D000328:Adult; D001363:Awards and Prizes; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D006176:Gynecology; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000302:isolation & purification*; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D009774:Obstetrics; D009845:Oligospermia / Q000821:virology; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000635:transmission / Q000821:virology*; D016133:Polymerase Chain Reaction; D027724:Reproductive Techniques, Assisted; D012661:Semen / Q000821:virology*; D012747:Sexual Partners; D013094:Spermatozoa / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D004279:DNA, Viral