title: Quantification of polyacetylenes in apiaceous plants by high-performance liquid chromatography coupled with diode array detection.
abstract: Polyacetylenes are known for their biofunctional properties in a wide range of organisms. In the present study, the most frequently occurring polyacetylenes, i.e. falcarinol, falcarindiol, and falcarindiol-3-acetate, were determined in six genera of the Apiaceae family. For this purpose, a straightforward and reliable method for the screening and quantification of the polyacetylenes using high-performance liquid chromatography coupled with diode array and mass spectrometric detection without tedious sample clean-up has been developed. Peak assignment was based on retention times, UV spectra, and mass spectral data. Quantification was carried out using calibration curves of authentic standards isolated from turnip-rooted parsley and Ligusticum mutellina, respectively. The references were unambiguously identified by Fourier transform-IR (FT-IR) spectroscopy, GC-MS, HPLC-MSn in the positive ionization mode, and 1H NMR and 13C NMR spectroscopy. To the best of our knowledge, the occurrence of falcarindiol-3-acetate in Anthriscus sylvestris and Pastinaca sativa has been reported for the first time. The data revealed great differences in the polyacetylene contents and varying proportions of individual compounds in the storage roots of Apiaceous plants. The results of the present study may be used as a suitable tool for authenticity control and applied to identify novel sources devoid or particularly rich in polyacetylenes, thus facilitating breeding programs for the selective enrichment and depletion of these plant secondary metabolites, respectively.
authors.affiliation: Institute of Food Science and Biotechnology, Chair of Plant Foodstuff Technology, Hohenheim University, Garbenstrasse 25, D-70599 Stuttgart, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Kramer
authors.forename: Andrea
authors.initials: A
authors.lastname: Mühleis
authors.forename: Jürgen
authors.initials: J
authors.lastname: Conrad
authors.forename: Martin
authors.initials: M
authors.lastname: Leitenberger
authors.forename: Uwe
authors.initials: U
authors.lastname: Beifuss
authors.forename: Reinhold
authors.initials: R
authors.lastname: Carle
authors.forename: Dietmar R
authors.initials: DR
authors.lastname: Kammerer
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D053279:Polyynes / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D053279:Polyynes