title: Catalytic specificity of human protein tyrosine kinases revealed by Peptide substrate profiling.
abstract: Out of the 90 human protein tyrosine kinases, 81 were assayed with short peptides derived from well-characterized [CDK1(Tyr15), IRS1(Tyr983), and JAK1(Tyr1023)] or generic [polyGlu:Tyr(4:1) and poly-Glu:Ala:Tyr(1:1:1)] substrates. As expected, the CDK1 peptide is a substrate for all Src family kinases. On the other hand, some of the activities are novel and lead to a better understanding of the function of certain kinases. Specifically, the CDK1 peptide is a substrate for many of the Eph family members. Interestingly, profiling of nearly all the human protein tyrosine kinases revealed a distinct pattern of selectivity towards the CDK1 and IRS1 peptides.
authors.affiliation: PerkinElmer Bio-discovery, 1744 William Street, Montreal, Quebec, H3J 1R4, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Blouin
authors.forename: Philippe
authors.initials: P
authors.lastname: Roby
authors.forename: Mathieu
authors.initials: M
authors.lastname: Arcand
authors.forename: Lucille
authors.initials: L
authors.lastname: Beaudet
authors.forename: Francesco
authors.initials: F
authors.lastname: Lipari