title: Immunohistochemical analysis of NaPi2b protein (MX35 antigen) expression and subcellular localization in human normal and cancer tissues.
abstract: OBJECTIVE
  To study the expression profile of the NaPi2b protein and its localization in breast, ovarian and lung cancer cells in relation to normal tissues adjacent to tumor.
  
  
  METHODS
  Immunohistochemical analysis with monoclonal antibody MX35 was applied for investigation of NaPi2b protein expression in breast, lung and ovarian carcinomas. Intensity of NaPi2b protein expression was calculated with semiquantitative scores.
  
  
  RESULTS
  NaPi2b (MX35) protein expression was detected in breast, lung and ovarian cancer cells and adjacent normal tissue. We have shown that in contrast to ovarian tumors in breast and lung tumors NaPi2b expression is down regulated comparing to correspondent normal tissues.
  
  
  CONCLUSIONS
  This study provides the data on the pattern of NaPi2b expression and cellular localization in breast, lung and ovarian cancers, which might be useful for understanding the mechanism of transport and maintenance of inorganic phosphate in cancer and normal cells, as well as for developing novel immunotherapeutic approaches based on MX35 monoclonal antibody.
authors.affiliation: Department of Cell Signaling, Institute of Molecular Biology and Genetics NAS of Ukraine, Kyiv, 03680, Ukraine.
authors.forename: R
authors.initials: R
authors.lastname: Kiyamova
authors.forename: M
authors.initials: M
authors.lastname: Shyian
authors.forename: V V
authors.initials: VV
authors.lastname: Lyzogubov
authors.forename: V S
authors.initials: VS
authors.lastname: Usenko
authors.forename: T
authors.initials: T
authors.lastname: Gout
authors.forename: V
authors.initials: V
authors.lastname: Filonenko
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000737:chemistry*; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb / Q000032:analysis*
chemical_list: C493557:SLC34A2 protein, human; D050614:Sodium-Phosphate Cotransporter Proteins, Type IIb