title: The intake of high fat diet with different trans fatty acid levels differentially induces oxidative stress and non alcoholic fatty liver disease (NAFLD) in rats.
abstract: BACKGROUND
  Trans-fatty acids (TFA) are known as a risk factor for coronary artery diseases, insulin resistance and obesity accompanied by systemic inflammation, the features of metabolic syndrome. Little is known about the effects on the liver induced by lipids and also few studies are focused on the effect of foods rich in TFAs on hepatic functions and oxidative stress. This study investigates whether high-fat diets with different TFA levels induce oxidative stress and liver dysfunction in rats.
  
  
  METHODS
  Male Wistar rats were divided randomly into four groups (n = 12/group): C receiving standard-chow; Experimental groups that were fed high-fat diet included 20% fresh soybean oil diet (FSO), 20% oxidized soybean oil diet (OSO) and 20% margarine diet (MG). Each group was kept on the treatment for 4 weeks.
  
  
  RESULTS
  A liver damage was observed in rats fed with high-fat diet via increase of liver lipid peroxidation and decreased hepatic antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase). The intake of oxidized oil led to higher levels of lipid peroxidation and a lower concentration of plasma antioxidants in comparison to rats fed with FSO. The higher inflammatory response in the liver was induced by MG diet. Liver histopathology from OSO and MG groups showed respectively moderate to severe cytoplasm vacuolation, hypatocyte hypertrophy, hepatocyte ballooning, and necroinflammation.
  
  
  CONCLUSIONS
  It seems that a strong relationship exists between the consumption of TFA in the oxidized oils and lipid peroxidation and non alcoholic fatty liver disease (NAFLD). The extent of the peroxidative events in liver was also different depending on the fat source suggesting that feeding margarine with higher TFA levels may represent a direct source of oxidative stress for the organism. The present study provides evidence for a direct effect of TFA on NAFLD.
authors.affiliation: Laboratory of Biochemistry, UR: "Human Nutrition and Metabolic Disorder" Faculty of Medicine of Monastir 5019, Tunisia. madiha.dhibi@hotmail.fr.
authors.forename: Madiha
authors.initials: M
authors.lastname: Dhibi
authors.forename: Faten
authors.initials: F
authors.lastname: Brahmi
authors.forename: Amira
authors.initials: A
authors.lastname: Mnari
authors.forename: Zohra
authors.initials: Z
authors.lastname: Houas
authors.forename: Issam
authors.initials: I
authors.lastname: Chargui
authors.forename: Linda
authors.initials: L
authors.lastname: Bchir
authors.forename: Noureddine
authors.initials: N
authors.lastname: Gazzah
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Alsaif
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammami