title: Expression of amnionless in mouse testes and Leydig cells.
abstract: Vitamin B(12) (cobalamin) deficiency results in atrophy of seminiferous tubules and aplasia of spermatozoa and spermatid. The transmembrane protein amnionless (AMN) directs endocytosis of cubilin with its ligand, contributing to intrinsic factor-vitamin B(12) absorption. To understand vitamin B(12) transport in testis, we analysed AMN expression in developing mouse testes and in Leydig cells and speculated the possible role of AMN in testis. In testes, Amn mRNA levels were low until 14 days post partum (pp) and markedly increased from puberty onwards. In the interstitium, Amn mRNA levels were low at 14 days pp and increased at puberty (28 days pp) together with 3-beta-hydroxysteroid dehydrogenase type 6 mRNA. Strong AMN immunoreactivity was observed in early spermatocytes from 7 days pp, suggesting that AMN participates in meiosis. In Leydig cells, AMN was not observed until 14 days pp but was strongly expressed after 28 days pp, suggesting a positive relationship between AMN expression and functional differentiation of adult Leydig cells. Together, AMN may participate in meiosis in early spermatocytes and in functional differentiation of adult Leydig cells through the mediation of vitamin B(12) transport in the mouse testes. This is the first report on AMN expression in the germ cells and soma of mammalian testes.
authors.affiliation: Department of Life Science and Institute for Natural Sciences, Hanyang University, Seoul, South Korea.
authors.forename: Y S
authors.initials: YS
authors.lastname: Oh
authors.forename: H Y
authors.initials: HY
authors.lastname: Park
authors.forename: M C
authors.initials: MC
authors.lastname: Gye
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000378:metabolism*
chemical_list: C424167:Amn protein, mouse; D017931:DNA Primers; D008565:Membrane Proteins; D012333:RNA, Messenger; D015096:3-Hydroxysteroid Dehydrogenases