title: Molecular imaging of a cancer-targeting theragnostics probe using a nucleolin aptamer- and microRNA-221 molecular beacon-conjugated nanoparticle.
abstract: MicroRNAs (miRNA, miR) have been reported as cancer biomarkers that regulate tumor suppressor genes. Hence, simultaneous detecting and inhibiting of miRNA function will be useful as a cancer theragnostics probe to minimize side effects and invasiveness. In this study, we developed a cancer-targeting therangostics probe in a single system using an AS1411 aptamer - and miRNA-221 molecular beacon (miR-221 MB)-conjugated magnetic fluorescence (MF) nanoparticle (MFAS miR-221 MB) to simultaneously target to cancer tissue, image intracellularly expressed miRNA-221 and treat miRNA-221-involved carcinogenesis. AS1411 aptamer-conjugated MF (MFAS) nanoparticles displayed a great selectivity and delivery into various cancer cell lines. The miR-221 MB detached from the MFAS miR-221 MB in the cytoplasm of C6 cells clearly imaged miRNA-221 biogenesis and simultaneously resulted in antitumor therapeutic effects by inhibiting miRNA function, indicating a successful astrocytoma-targeting theragnostics. MFAS miRNA MB can be easily applied to other cancers by simply changing a targeted miRNA highly expressed in cancers.
authors.affiliation: Department of Pharmacy, College of Pharmacy, CHA University, Gyeonggi-do 487-010, South Korea.
authors.forename: Jin Kyeoung
authors.initials: JK
authors.lastname: Kim
authors.forename: Kyung-Ju
authors.initials: KJ
authors.lastname: Choi
authors.forename: Minhyung
authors.initials: M
authors.lastname: Lee
authors.forename: Mi-hee
authors.initials: MH
authors.lastname: Jo
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000737:chemistry*; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use*; D009369:Neoplasms / Q000188:drug therapy*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000627:therapeutic use*; D010750:Phosphoproteins / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D000097796:Nucleolin
chemical_list: D052157:Aptamers, Nucleotide; D035683:MicroRNAs; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C513936:AGRO 100