title: MS3 eliminates ratio distortion in isobaric multiplexed quantitative proteomics.
abstract: Quantitative mass spectrometry-based proteomics is highly versatile but not easily multiplexed. Isobaric labeling strategies allow mass spectrometry-based multiplexed proteome quantification; however, ratio distortion owing to protein quantification interference is a common effect. We present a two-proteome model (mixture of human and yeast proteins) in a sixplex isobaric labeling system to fully document the interference effect, and we report that applying triple-stage mass spectrometry (MS3) almost completely eliminates interference.
authors.affiliation: Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Ting
authors.forename: Ramin
authors.initials: R
authors.lastname: Rad
authors.forename: Steven P
authors.initials: SP
authors.lastname: Gygi
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Haas
mesh_terms: D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000737:chemistry