title: Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins.
abstract: Production of recombinant protein in mammalian cells is time-consuming, labor-intensive and costly. While seeking to overcome these limitations, we discovered that Vaccinia virus has the innate ability to transfer exogenous plasmid DNA into mammalian cells during the infection process. Parameters influencing the efficiency of this event were characterized and a quick, simple and inexpensive way to produce eukaryotic proteins was established.
authors.affiliation: Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15, rue René Descartes, F-67084 Strasbourg, France. jester_brian@hotmail.com
authors.forename: Brian C
authors.initials: BC
authors.lastname: Jester
authors.forename: Robert
authors.initials: R
authors.lastname: Drillien
authors.forename: Marc
authors.initials: M
authors.lastname: Ruff
authors.forename: Catherine
authors.initials: C
authors.lastname: Florentz
mesh_terms: D000818:Animals; D001709:Biotechnology / Q000379:methods; D004247:DNA / Q000235:genetics; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection; D014615:Vaccinia / Q000235:genetics / Q000821:virology; D014616:Vaccinia virus / Q000502:physiology*; D016662:Virus Integration
chemical_list: D011994:Recombinant Proteins; D004247:DNA