title: Preparation of ciprofloxacin-coated zinc oxide nanoparticles and their antibacterial effects against clinical isolates of Staphylococcus aureus and Escherichia coli.
abstract: In the present research study, ciprofloxacin-coated zinc oxide nanoparticles were prepared using a precipitation method. The nature of interactions between zinc oxide nanoparticles and ciprofloxacin (CAS 85721-33-1) was studied by Fourier transform infrared spectroscopy. The results show that the carbonyl group in ciprofloxacin is actively involved in forming chemical--rather than physical--bonds with zinc oxide nanoparticles. Also the antibacterial activity of free zinc oxide nanoparticles and ciprofloxacin-coated zinc oxide nanoparticles have been evaluated against different clinical isolates of Staphylococcus aureus and Escherichia coli. The free zinc oxide nanoparticles did not show potent antibacterial activity against all test strains. In contrast, only the low concentrations of ciprofloxacin-coated zinc oxide nanoparticles (equivalent to the sub-minimum inhibitory concentrations of pure ciprofloxacin) considerably enhanced the antibacterial activity of zinc oxide nanoparticles against different isolates of Staphylococcus aureus and Escherichia coli (4 to 32 fold increase). The result is of particular value, since it demonstrates that, by using biocompatible zinc oxide nanoparticles in combination therapy, lower amounts of antibiotics may be needed.
authors.affiliation: Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Seif
authors.forename: Zahra Bahri
authors.initials: ZB
authors.lastname: Kazempour
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Pourmand
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Shahverdi
authors.forename: Massoud
authors.initials: M
authors.lastname: Amanlou
authors.forename: Roya
authors.initials: R
authors.lastname: Bazl
authors.forename: Zeynab Esmail
authors.initials: ZE
authors.lastname: Nazari
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Shahverdi
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000494:pharmacology*; D004339:Drug Compounding; D004926:Escherichia coli / Q000187:drug effects*; D005079:Excipients; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects*; D013499:Surface Properties; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D005079:Excipients; D002939:Ciprofloxacin; D015034:Zinc Oxide