title: Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks.
abstract: A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.
authors.affiliation: Laboratoire de Phytotechnologie EA 3900-BioPI, University of Picardie Jules Verne, Amiens, France.
authors.forename: Ophélie
authors.initials: O
authors.lastname: Fliniaux
authors.forename: Gwenaelle
authors.initials: G
authors.lastname: Gaillard
authors.forename: Antoine
authors.initials: A
authors.lastname: Lion
authors.forename: Dominique
authors.initials: D
authors.lastname: Cailleu
authors.forename: François
authors.initials: F
authors.lastname: Mesnard
authors.forename: Fotini
authors.initials: F
authors.lastname: Betsou
mesh_terms: D000328:Adult; D018070:Biological Specimen Banks; D015415:Biomarkers; D002498:Centrifugation; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D055442:Metabolome; D008875:Middle Aged; D019906:Nuclear Magnetic Resonance, Biomolecular; D044967:Serum / Q000737:chemistry* / Q000378:metabolism; D013048:Specimen Handling / Q000379:methods*; D055815:Young Adult
chemical_list: D015415:Biomarkers