title: Characterization of the lipopolysaccharide O-antigen of Cronobacter turicensis HPB3287 as a polysaccharide containing a 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (legionaminic acid) residue.
abstract: Cronobacter turicensis, previously known as Enterobacter sakazakii, is a Gram-negative opportunistic food-borne pathogen that has been reported as a cause of life-threatening neonatal infections. From chemical and physical analyses involving composition analysis, methylation, two-dimensional high-resolution nuclear magnetic resonance, and mass spectrometry methods, the antigenic O-polysaccharide in the smooth-type lipopolysaccharide of C. turicensis (strain HPB 3287) was determined to be a high molecular mass polymer of a repeating pentasaccharide unit composed of D-galactose, D-glucose, 2-acetamido-2-deoxy-D-galactose, and 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-galacto-non-2-ulosonic acid (legionaminic acid), in a molar ratio 2:1:1:1, and having the structure: [see formula in text].
authors.affiliation: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6.
authors.forename: Leann L
authors.initials: LL
authors.lastname: MacLean
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Franco
authors.initials: F
authors.lastname: Pagotto
authors.forename: Malcolm B
authors.initials: MB
authors.lastname: Perry
mesh_terms: D002240:Carbohydrate Sequence; D044083:Cronobacter sakazakii / Q000737:chemistry*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012794:Sialic Acids / Q000737:chemistry*
chemical_list: C110570:5,7-diacetamido-8-O-acetyl-3,5,7,9-tetradeoxy-glycero-talo-nonulosonic acid; D008070:Lipopolysaccharides; D019081:O Antigens; D012794:Sialic Acids