title: [Conjugation of Plasmodium falciparum Pfs25 to Pseudomonas aeruginosa ExoProtein A with different chemical linkers].
abstract: OBJECTIVE
  To conjugate Plasmodium falciparum Pfs25 to recombinant Pseudomonas aeruginosa Exo-Protein A (rEPA) and test the effect of conjugation using different chemical linkers.
  
  
  METHODS
  The Pfs25 was thiolated by NAHT (DL-N-acetylhomocysteine thiolactone), whereas the carrier protein rEPA was modified by Sulfo-EMCS, EMCH, SBAP and Sulfo-SIAB respectively. The Pfs25-rEPA conjugates were formed by incubating the thiolated Pfs25 with modified rEPA under certain conditions. Coomassie blue stained SDS-PAGE was performed to examine the results of the conjugation.
  
  
  RESULTS
  The Pfs25 was successfully conjugated to the carrier protein rEPA by the linkers used in this study. For the addition of a chemical group onto the protein, the reaction between the groups of primary amine and NHS (N-hydroxysuccinimide) ester was more efficient than the reaction between the groups of carboxyl and hydrazide with EDC as a crosslinker, for the formation of a conjugate, the reaction between the groups of maleimide and free sulfhydryl was more efficient than the reaction between the groups of haloacetyl and free sulfhydryl.
  
  
  CONCLUSIONS
  The Pfs25 can be conjugated to the rEPA by the chemical linkers with different conjugation efficiency and coupling proteins.
authors.affiliation: Department of Rheumatology and Immunology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
mesh_terms: D036002:ADP Ribose Transferases / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D005098:Exotoxins / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*; D037521:Virulence Factors / Q000737:chemistry*; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D015800:Protozoan Proteins; D013876:Thiophenes; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C071284:Pfs25 protein, Plasmodium falciparum; C000486:citiolone