title: Phylogenetic and pathotypical analysis of two virulent Newcastle disease viruses isolated from domestic ducks in China.
abstract: Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to genotype IX. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that both isolates contained the virulent motif (112)RRQK/RRF(117) at the cleavage site. The two NDVs had severe pathogenicity in fully susceptible chickens, resulting in 100% mortality. One of the isolates also demonstrated some pathogenicity in domestic ducks. The present study suggests that more than one genotype of NDV circulates in domestic ducks in China and viral transmission may occur among chickens and domestic ducks.
authors.affiliation: Key Laboratory of Animal Epidemiology and Zoonosis, College of Veterinary Medicine, Ministry of Agriculture, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Wang
authors.forename: Changguang
authors.initials: C
authors.lastname: Zhao
authors.forename: Dehua
authors.initials: D
authors.lastname: Liu
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Hu
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhao
authors.forename: Guozhong
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics / Q000821:virology; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D004372:Ducks / Q000235:genetics / Q000821:virology*; D009521:Newcastle Disease / Q000453:epidemiology / Q000473:pathology* / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger