title: Molecular identification of a new begomovirus associated with yellow mosaic disease of Jatropha gossypifolia in India.
abstract: Yellow mosaic disease was observed on Jatropha gossypifolia plants growing in Kathaupahadi, Madhya Pradesh, India, and whiteflies (Bemisia tabaci) were found in the vicinity. Association of a new begomovirus with yellow mosaic disease of J. gossypifolia has been detected by PCR using begomovirus DNA-A-specific primers. The complete DNA-A genome (~2.7 kb) of this virus isolate was amplified by rolling-circle amplification (RCA) followed by digestion with Bam HI. The ~2.7-kb amplicons was cloned and sequenced, and the data obtained were submitted to GenBank under accession numbers FJ177030. The genome of the virus isolate consisted of six open reading frames (ORFs): V2 (pre-coat protein) and V1 (coat protein) in the virion sense and C3 (REn protein), C2 (TrAP protein), C1 (replication-associated protein) and C4 (C4 protein) in the complementary sense. BLASTn analysis of the nucleotide sequence (2757 nt) of the viral genome (FJ177030) showed 84-85% identity and a distinct phylogenetic relationship with DNA-A of tomato leaf curl virus-Bangalore II (U38239) and tomato leaf curl Karnataka virus (AY754812). Based on its 85% sequence identity to all other begomoviruses known to date and ICTV species demarcating criteria (< 88% identity), the name Jatropha yellow mosaic India virus (JYMIV) is proposed. JYMIV is considered to be monopartite, as neither DNA-B nor DNA-β components associated with begomoviruses were detected.
authors.affiliation: Plant Molecular Virology, CSIR-National Botanical Research Institute, Lucknow, UP, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Snehi
authors.forename: S K
authors.initials: SK
authors.lastname: Raj
authors.forename: M S
authors.initials: MS
authors.lastname: Khan
authors.forename: V
authors.initials: V
authors.lastname: Prasad
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006430:Hemiptera / Q000821:virology; D007194:India; D007303:Insect Vectors / Q000821:virology; D031286:Jatropha / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*
chemical_list: D004279:DNA, Viral