title: Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production.
abstract: The rabies Flury Low Egg Passage virus (LEP) has been widely used as a seed virus to generate inactive vaccine. Here, we established a reverse genetic system for LEP and generated a recombinant LEP virus (rLEP-G) that carries two identical G genes. This recombinant virus showed similar properties to those of LEP with respect to in vitro growth, neurotropism index, and virulence in mice. rLEP-G produced 4.3-fold more G protein than did LEP in BHK-21 cells. The inactivated vaccine generated from rLEP-G induced significantly higher virus neutralization titers in mice and dogs than those produced in response to LEP-derived vaccine. Our results suggest that rLEP-G is an improved seed virus candidate for inactivated rabies virus vaccine manufacture.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Tao
authors.forename: Jinying
authors.initials: J
authors.lastname: Ge
authors.forename: Xijun
authors.initials: X
authors.lastname: Wang
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wen
authors.forename: Hongyue
authors.initials: H
authors.lastname: Zhai
authors.forename: Tao
authors.initials: T
authors.lastname: Hua
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhao
authors.forename: Dongni
authors.initials: D
authors.lastname: Kong
authors.forename: Chinglai
authors.initials: C
authors.lastname: Yang
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D004285:Dogs; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D007114:Immunization; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011818:Rabies / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D011819:Rabies Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D011820:Rabies virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014161:Transduction, Genetic; D015164:Vaccines, Inactivated / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014774:Virulence / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D015164:Vaccines, Inactivated; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus