title: Electrochemical synthesis of gold nanostructure modified electrode and its development in electrochemical DNA biosensor.
abstract: In this article, gold nanostructure modified electrodes were achieved by a simple one-step electrodeposition method. The morphologies of modified electrodes could be easily controlled by changing the pH of HAuCl(4) solution. The novel nanoflower-like particles with the nanoplates as the building blocks could be interestingly obtained at pH 5.0. The gold nanoflower modified electrodes were then used for the fabrication of electrochemical DNA biosensor. The DNA biosensor fabrication process was characterized by cyclic voltammetry and electrochemical impedance spectroscopy with the use of ferricyanide as an electrochemical redox indicator. The DNA immobilization and hybridization on gold nanoflower modified electrode was studied with the use of [Ru(NH(3))(6)](3+) as a hybridization indicator. The electrochemical DNA biosensor shows a good selectivity and sensitivity toward the detection of target DNA. A detection limit of 1 pM toward target DNA could be obtained.
authors.affiliation: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Han
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.forename: Changzhi
authors.initials: C
authors.lastname: Zhao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*
chemical_list: D006046:Gold; D004247:DNA