title: Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra.
abstract: Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.
authors.affiliation: Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. f.a.a.mulder@rug.nl
authors.forename: Frans A A
authors.initials: FA
authors.lastname: Mulder
authors.forename: Renee
authors.initials: R
authors.lastname: Otten
authors.forename: Ruud M
authors.initials: RM
authors.lastname: Scheek
mesh_terms: D016477:Artifacts; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results