title: Increased circulating levels of SDF-1 (CXCL12) in type 2 diabetic patients are correlated to disease state but are unrelated to polymorphism of the SDF-1β gene in the Iranian population.
abstract: Several environmental and genetic factors are believed to influence the onset of diabetes and its complications. It has also been established that cytokines play a key role in the pathogenesis of type 2 diabetes. Previous studies have revealed that the polymorphism at the stromal-derived factor 1β (SDF-1β) 3'A regulates the expression of SDF-1 (CXCL12). This study was aimed to explore this polymorphism in parallel with SDF-1 serum levels in type 2 diabetic patients. In this assessment, peripheral blood samples were collected from 200 type 2 diabetic patients and 200 healthy controls. DNA was extracted, and a PCR-RFLP screening was applied to examine the SDF-1β 3'A polymorphism. We also applied the ELISA technique to measure serum levels of SDF-1. Our results showed that there were no significant correlations between SDF-1β 3'Α polymorphism in type 2 diabetic patients when compared to controls. However, our results showed that the serum levels of SDF-1 were significantly increased in the patients when compared to controls. Based on the results of this study, we concluded that SDF-1β 3'Α polymorphism does not play a role in the pathogenesis of type 2 diabetes but that elevated serum levels of SDF-1 may be important for the etiology of type 2 diabetes but are unrelated to the SDF-1β 3'Α polymorphism.
authors.affiliation: Department of Pediatrics, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Derakhshan
authors.forename: Mohammad Kazemi
authors.initials: MK
authors.lastname: Arababadi
authors.forename: Zahra
authors.initials: Z
authors.lastname: Ahmadi
authors.forename: Mojgan Noroozi
authors.initials: MN
authors.lastname: Karimabad
authors.forename: Vajihe Akbarpour
authors.initials: VA
authors.lastname: Salehabadi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Abedinzadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Khorramdelazad
authors.forename: Parisa
authors.initials: P
authors.lastname: Balaei
authors.forename: Derek
authors.initials: D
authors.lastname: Kennedy
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Hassanshahi
mesh_terms: D000328:Adult; D054377:Chemokine CXCL12 / Q000097:blood* / Q000235:genetics*; D004247:DNA / Q000032:analysis; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran; D008297:Male; D020641:Polymorphism, Single Nucleotide
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D004247:DNA