title: Development of lysolipid-based thermosensitive liposomes for delivery of high molecular weight proteins.
abstract: The purpose of the present investigation was to determine the feasibility of using lysolipid-based thermal sensitive liposomes (145nm) for high molecular weight molecule delivery. Fluorescein isothiocyanate conjugate-albumin was used as a model drug (MW 66kDa). Thermal sensitive liposomes, which encapsulated the protein were prepared using a passive encapsulation methodology involving freeze-thawing cycles followed by extrusion. In vitro release studies at various temperatures indicated rapid release behaviour of the encapsulated protein at 42 and 44.5°C but a good stability at 37.5°C. The current findings suggest that lysolipid-based thermal sensitive liposomes can be used to deliver high molecular weight molecules.
authors.affiliation: Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, UK. xin.zhang09@imperial.ac.uk
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Paul F
authors.initials: PF
authors.lastname: Luckham
authors.forename: Alun D
authors.initials: AD
authors.lastname: Hughes
authors.forename: Simon
authors.initials: S
authors.lastname: Thom
authors.forename: Xiao Yun
authors.initials: XY
authors.lastname: Xu
mesh_terms: D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D006358:Hot Temperature; D008081:Liposomes / Q000737:chemistry*; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D008081:Liposomes; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate