title: Comparison of beer quality attributes between beers brewed with 100% barley malt and 100% barley raw material.
abstract: BACKGROUND
  Brewing with 100% barley using the Ondea® Pro exogenous brewing enzyme product was compared to brewing with 100% barley. The use of barley, rather than malt, in the brewing process and the consequences for selected beer quality attributes (foam formation, colloidal stability and filterability, sensory differences, protein content and composition) was considered.
  
  
  RESULTS
  The quality attributes of barley, malt, kettle-full-wort, cold wort, unfiltered beer and filtered beer were assessed. A particular focus was given to monitoring changes in the barley protein composition during the brewing process and how the exogenous OndeaPro® enzymes influenced wort protein composition. All analyses were based on standard brewing methods described in ASBC, EBC or MEBAK. To monitor the protein changes two-dimensional polyacrylamide gel electrophoresis was used.
  
  
  CONCLUSIONS
  It was shown that by brewing beer with 100% barley and an appropriate addition of exogenous Ondea® Pro enzymes it was possible to efficiently brew beer of a satisfactory quality. The production of beers brewed with 100% barley resulted in good process efficiency (lautering and filtration) and to a final product whose sensory quality was described as light, with little body and mouthfeel, very good foam stability and similar organoleptic qualities compared to conventional malt beer. In spite of the sensory evaluation differences could still be seen in protein content and composition.
authors.affiliation: Lehrstuhl für Brau- und Getränketechnologie, Technische Universität München, Freising, Germany. elisabeth.steiner@wzw.tum.de
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Steiner
authors.forename: Andrea
authors.initials: A
authors.lastname: Auer
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
authors.forename: Martina
authors.initials: M
authors.lastname: Gastl
mesh_terms: D001515:Beer / Q000032:analysis*; D055598:Chemical Phenomena; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism; D005285:Fermentation; D005858:Germany; D018525:Germination; D001467:Hordeum / Q000737:chemistry* / Q000201:enzymology / Q000254:growth & development; D006801:Humans; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D010865:Pilot Projects; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D011786:Quality Control; D012639:Seeds / Q000737:chemistry* / Q000254:growth & development; D012677:Sensation; D013649:Taste; D014783:Viscosity
chemical_list: D004044:Dietary Proteins; D010446:Peptide Fragments; D010940:Plant Proteins; D006867:Hydrolases