title: [Research of the essential oil of Plumeria rubra var. actifolia from Laos by supercritical carbon dioxide extraction].
abstract: The orthogonal test and the supercritical carbon dioxide fluid extraction were used for optimizing the extraction of the essential oil from Plumeria rubra var. actifolia for the first time. Compared with the steam distillation, the optimal operation parameter of extraction was as follows: extraction pressure 25 MPa, extraction temperature 45 degrees C; separator I pressure 12 MPa, separator I temperature 55 degrees C; separator II pressure 6 MPa, separator II temperature 30 degrees C. Under this condition the yield of the essential oil was 5.8927%. The components were separated and identified by GC-MS. 53 components of Plumeria rubra var. actifolia measured by SFE method were identified and determined by normalization method. The main components were 1, 6, 10-dodecatrien-3-ol, 3, 7, 11-trimethyl, benzoic acid, 2-hydroxy-, phenylmethyl ester, 1, 2-benzenedicarboxylic acid, bis(2-methylpropyl) ester,etc.. 1, 2-Benzenedicarboxylic acid, bis (2-methylpropyl) este. took up 66.11% of the total amount, and there was much difference of the results from SD method.
authors.affiliation: Guangzhou University of TCM, Guangzhou 510405, China.
authors.forename: Xin-Yu
authors.initials: XY
authors.lastname: Xiao
authors.forename: Long-Hai
authors.initials: LH
authors.lastname: Cui
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Fa-Huan
authors.initials: FH
authors.lastname: Ge
mesh_terms: D000438:Alcohols / Q000032:analysis / Q000737:chemistry; D029062:Apocynaceae / Q000737:chemistry*; D002245:Carbon Dioxide; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004952:Esters / Q000032:analysis / Q000737:chemistry; D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D007812:Laos; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D011312:Pressure; D013696:Temperature
chemical_list: D000438:Alcohols; D004952:Esters; D009822:Oils, Volatile; D002245:Carbon Dioxide