title: Q(γ-H2AX), an analysis method for partial-body radiation exposure using γ-H2AX in nonhuman primate lymphocytes.
abstract: We previously used the γ-H2AX assay as a biodosimeter for total-body-irradiation (TBI) exposure (γ-rays) in a rhesus macaque (Macaca mulatta) model. Utilizing peripheral blood lymphocytes and plucked hairs, we obtained statistically significant γ-H2AX responses days after total-body exposure to 1-8.5 Gy ((60)Co γ-rays at 55 cGy min(-1)). Here, we introduce a partial-body exposure analysis method, Q(γ-H2AX), which is based on the number of γ-H2AX foci per damaged cells as evident by having one or more γ-H2AX foci per cell. Results from the rhesus monkey - TBI study were used to establish Q(γ-H2AX) dose-response calibration curves to assess acute partial-body exposures. γ-H2AX foci were detected in plucked hairs for several days after in vivo irradiation demonstrating this assay's utility for dose assessment in various body regions. The quantitation of γ-H2AX may provide a robust biodosimeter for analyzing partial body exposures to ionizing radiation in humans.
authors.affiliation: NIH, NCI, CCR, Laboratory of Molecular Pharmacology, Bethesda, MD 20892, U.S.A.
authors.forename: Christophe E
authors.initials: CE
authors.lastname: Redon
authors.forename: Asako J
authors.initials: AJ
authors.lastname: Nakamura
authors.forename: Ksenia
authors.initials: K
authors.lastname: Gouliaeva
authors.forename: Arifur
authors.initials: A
authors.lastname: Rahman
authors.forename: William F
authors.initials: WF
authors.lastname: Blakely
authors.forename: William M
authors.initials: WM
authors.lastname: Bonner