title: New aspects of RNA processing in prokaryotes.
abstract: The pivotal role of posttranscriptional gene regulation is strongly underlined by genome-wide analyses showing strikingly low correlation between mRNA and protein levels in bacterial and archaeal cells. The stability of an mRNA and its availability for translation contribute to posttranscriptional gene regulation, and are determined by the following factors: i) the cell-specific set of ribonucleases and related proteins, ii) regulatory RNAs, and iii) the sequence and structural features of the RNA molecule itself. High-resolution analyses of whole prokaryotic transcriptomes allow comprehensive mapping of processed transcripts, detection of essentially all expressed regulatory RNAs, and monitoring of the global impact of ribonucleases and other processing factors. This opens new perspectives for the understanding of the molecular mechanisms responsible for mRNA decay in prokaryotes.
authors.affiliation: Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany. Elena.Evguenieva-Hackenberg@mikro.bio.uni-giessen.de
authors.forename: Elena
authors.initials: E
authors.lastname: Evguenieva-Hackenberg
authors.forename: Gabriele
authors.initials: G
authors.lastname: Klug
mesh_terms: D015870:Gene Expression; D019848:Gene Expression Regulation, Archaeal; D015964:Gene Expression Regulation, Bacterial; D011387:Prokaryotic Cells / Q000378:metabolism / Q000502:physiology*; D012323:RNA Processing, Post-Transcriptional; D019642:RNA, Archaeal / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D019642:RNA, Archaeal; D012329:RNA, Bacterial