title: Biochemical characterization of the very long-chain fatty acid elongase ELOVL7.
abstract: Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated K(m) values toward C18:3(n-3)-CoA and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.
authors.affiliation: Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-ku, Sapporo, Japan.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Naganuma
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Sato
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sassa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ohno
authors.forename: Akio
authors.initials: A
authors.lastname: Kihara
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002384:Catalysis; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008316:Malonyl Coenzyme A / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism
chemical_list: C000630949:ELOVL1 protein, human; C000630957:ELOVL7 protein, human; D005227:Fatty Acids; D008316:Malonyl Coenzyme A; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases