title: Dual- and triple-color fluorospot.
abstract: Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The method is limited, however, in that only one cytokine at a time is assessed. Fluorospot is based on the principle of ELISPOT, but facilitates the analysis of single cells secreting several cytokines, e.g., polyfunctional T cells, suggested to be of protective importance in various infectious diseases. By detecting each cytokine with a specific fluorophore and analyzing differentially colored spots by fluorophore--specific filter systems, cells producing single or multiple cytokines are identified. Fluorospot maintains the simplicity and sensitivity of the ELISPOT while taking the analysis a step forward toward multiplex analysis.
authors.affiliation: MABTECH AB, Nacka Strand, Sweden. niklas@mabtech.com
authors.forename: Niklas
authors.initials: N
authors.lastname: Ahlborg
authors.forename: Bernt
authors.initials: B
authors.lastname: Axelsson
mesh_terms: D016207:Cytokines / Q000032:analysis* / Q000276:immunology; D058501:Enzyme-Linked Immunospot Assay / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D016207:Cytokines