title: [Fragment of the gene encoding chymotrypsin and trypsin inhibitor protein of potato tubers].
abstract: Product of polymerase chain reaction designated as PKPIJ-B was isolated after amplification from genomic DNA of potato (Solarium tuberosum L., Zhukov Jubilee cultivar) using the designed primers. Nucleotide sequence of PKPIJ-B was determined and amino acid sequence of protein was restored. Analysis of this sequence has allowed us to suggest that isolated gene fragment encodes chymotrypsin and trypsin inhibitor protein (PKCI-23 potato Kunitz-type chymotrypsin inhibitor) of potato tubers.
authors.forename: I A
authors.initials: IA
authors.lastname: Parfenov
authors.forename: T A
authors.initials: TA
authors.lastname: Revina
authors.forename: P P
authors.initials: PP
authors.lastname: Pashkovskiĭ
authors.forename: N L
authors.initials: NL
authors.lastname: Radiukina
authors.forename: T A
authors.initials: TA
authors.lastname: Valueva
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002918:Chymotrypsin / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D011110:Polymorphism, Genetic; D020033:Protein Isoforms / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D017931:DNA Primers; D010940:Plant Proteins; D020033:Protein Isoforms; D014361:Trypsin Inhibitors; D004247:DNA; D002918:Chymotrypsin; D014357:Trypsin