title: Lack of an association between a single nucleotide polymorphism in the bovine myogenic determination 1 (MyoD1) gene and meat quality traits in indigenous Chinese cattle breeds.
abstract: The  myogenic determination 1 (MyoD1) gene is a member of the MyoD gene family. It  encodes for skeletal muscle-specific transcription factors containing highly  conserved basic helix-loop-helix regions that perform important roles in the  initiation, maintenance, and regulation of phenotypic traits. We investigated a  new single nucleotide polymorphism (SNP) in the MyoD1 gene to evaluate whether  this polymorphism affects meat quality traits in five Chinese indigenous cattle  breeds, namely Qinchuan (QC), Xia-Nan (XN), Nan-yang (NY), Luxi (LX), and  Jia-xian red (JXR). A C→G transversion at position 624 was detected in exon 1  of the MyoD1 gene; it causes an amino acid substitution ((624)serine/(624)cysteine).  Least squares analysis showed that this SNP is not significantly associated  with back fat thickness, eye muscle area, intramuscular fat, or marbling. The  A/B allelic frequencies in the five breeds were 0.810/0.189, 0.779/0.220,  0.768/0.231, 0.820/0.180, and 0.801/0.198, respectively. Based on the χ(2) test,  the genotype distributions of four cattle breeds (LX, NY, QC, and XN) did not  agree with Hardy-Weinberg equilibrium (P < 0.05); one breed (JXR) did not  deviate significantly from Hardy-Weinberg equilibrium (P > 0.05). The  genotypic frequencies among all five cattle breeds showed moderate diversity  (0.25 < polymorphism information content < 0.5). We concluded that the  C624G SNP of the MyoD1 gene does not influence meat quality traits in  indigenous Chinese cattle breeds; however, this SNP could be included in breed  composition and population admixture analyses due to the marked differences in  allelic frequencies among these five breeds.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, PR China. javed.asrl_nwsuaf@live.com
authors.forename: J A
authors.initials: JA
authors.lastname: Ujan
authors.forename: L S
authors.initials: LS
authors.lastname: Zan
authors.forename: H B
authors.initials: HB
authors.lastname: Wang
authors.forename: S A
authors.initials: SA
authors.lastname: Ujan
authors.forename: C
authors.initials: C
authors.lastname: Adoligbe
authors.forename: H C
authors.initials: HC
authors.lastname: Wang
authors.forename: S F
authors.initials: SF
authors.lastname: Biao
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001823:Body Composition / Q000235:genetics*; D002417:Cattle; D002681:China; D005787:Gene Frequency; D014644:Genetic Variation; D005838:Genotype; D008460:Meat; D018482:Muscle, Skeletal; D017570:MyoD Protein / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein