title: Efficient RNA synthesis by in vitro transcription of a triazole-modified DNA template.
abstract: A DNA strand containing a triazole phosphodiester mimic is an efficient template for in vitro transcription. This is the first demonstration of transcription through a heavily modified DNA backbone linkage and it suggests that click-ligated DNA could be useful for the direct synthesis of biologically active RNA and proteins.
authors.affiliation: School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, UK.
authors.forename: Afaf H
authors.initials: AH
authors.lastname: el-Sagheer
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D057930:Click Chemistry; D004247:DNA / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis*; D014158:Transcription, Genetic; D014230:Triazoles / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D014230:Triazoles; D014764:Viral Proteins; D012313:RNA; D004247:DNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases