title: Electrophysiological analysis of G protein-coupled receptors in mammalian neurons.
abstract: This unit describes general techniques that are useful for recording electrophysiological responses that are mediated via the activation of G-protein coupled receptors (GPCRs). It includes a brief description of preparations, but focuses primarily on experiments using hippocampal brain slice preparations. Techniques for the preparation of brain slices, electrodes, filling solutions, and the recording protocols that are suitable for assessing the activity of GPCRs using electrophysiological techniques are summarized, and various protocols for the activation of these receptors are discussed.
authors.affiliation: Veterans Administration Medical Research Service, Denver, Colorado, USA.
authors.forename: W R
authors.initials: WR
authors.lastname: Proctor
authors.forename: T V
authors.initials: TV
authors.lastname: Dunwiddie
mesh_terms: D000818:Animals; D055724:Electrophysiological Phenomena / Q000502:physiology*; D005260:Female; D008564:Membrane Potentials / Q000502:physiology; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*; D014981:Xenopus
chemical_list: D043562:Receptors, G-Protein-Coupled