title: Molecular detection and cloning of thermostable hemolysin gene from Aeromonas hydrophila.
abstract: Aeromonas hydrophila is a major bacterial pathogen associated with hemorrhagic septicemia in aquatic and terrestrial animals including humans. There is an urgent need to develop molecular and immunological assays for rapid, specific and sensitive diagnosis. A new set of primers has been designed for detection of thermostable hemolysin (TH) gene (645 bp) from A. hydrophila, and sensitivity limit for detection of TH gene was 5 pg. The TH gene was cloned, sequenced and analyzed. The G+C content was 68.06%; and phylogeny was constructed using TH protein sequences which had significant homology with those for thermostable and other hemolysins present in several bacterial pathogens. In addition, we have predicted the four and eight T-cell epitopes for MHC class I and II alleles, respectively. These results provide new insight for TH protein containing antigenic epitopes that can be used in immunoassays and also designing of thermostable vaccines.
authors.affiliation: Programme d'Epigénomique, Institute of Systems and Synthetic Biology, Universite d'Evry Val d'Essonne-Genopole-CNRS UPS3201, 5 rue Henri Desbrueres, 91030 Evry, France. vijaisingh15@gmail.com
authors.forename: Vijai
authors.initials: V
authors.lastname: Singh
authors.forename: Indra
authors.initials: I
authors.lastname: Mani
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Chaudhary
authors.forename: Pallavi
authors.initials: P
authors.lastname: Somvanshi
mesh_terms: D016980:Aeromonas hydrophila / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D003001:Cloning, Molecular; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000175:diagnosis / Q000382:microbiology; D005399:Fishes / Q000382:microbiology; D005805:Genes, MHC Class I / Q000276:immunology; D005802:Genes, MHC Class II / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000662:veterinary; D006460:Hemolysin Proteins / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D010802:Phylogeny; D055550:Protein Stability; D017386:Sequence Homology, Amino Acid
chemical_list: D018984:Epitopes, T-Lymphocyte; D006460:Hemolysin Proteins