title: Gibberellin A1 metabolism contributes to the control of photoperiod-mediated tuberization in potato.
abstract: Some potato species require a short-day (SD) photoperiod for tuberization, a process that is negatively affected by gibberellins (GAs). Here we report the isolation of StGA3ox2, a gene encoding a GA 3-oxidase, whose expression is increased in the aerial parts and is repressed in the stolons after transfer of photoperiod-dependent potato plants to SD conditions. Over-expression of StGA3ox2 under control of constitutive or leaf-specific promoters results in taller plants which, in contrast to StGA20ox1 over-expressers previously reported, tuberize earlier under SD conditions than the controls. By contrast, StGA3ox2 tuber-specific over-expression results in non-elongated plants with slightly delayed tuber induction. Together, our experiments support that StGA3ox2 expression and gibberellin metabolism significantly contribute to the tuberization time in strictly photoperiod-dependent potato plants.
authors.affiliation: Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB, Bellaterra, Spain. jordi.bou@cragenomica.es
authors.forename: Jordi
authors.initials: J
authors.lastname: Bou-Torrent
authors.forename: Jaime F
authors.initials: JF
authors.lastname: Martínez-García
authors.forename: José Luis
authors.initials: JL
authors.lastname: García-Martínez
authors.forename: Salomé
authors.initials: S
authors.lastname: Prat
mesh_terms: D000595:Amino Acid Sequence; D053898:Biosynthetic Pathways; D002940:Circadian Rhythm; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism*; D007527:Isoenzymes / Q000145:classification / Q000235:genetics / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000145:classification / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D017440:Photoperiod; D010802:Phylogeny; D035261:Plant Components, Aerial / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D035281:Plant Tubers / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005875:Gibberellins; D007527:Isoenzymes; C422660:gibberellin A1; D006899:Mixed Function Oxygenases; C108186:gibberellin 3beta-hydroxylase