title: Early Cerebral Hemodynamic, Metabolic, and Histological Changes in Hypoxic-Ischemic Fetal Lambs during Postnatal Life.
abstract: The hemodynamic, metabolic, and biochemical changes produced during the transition from fetal to neonatal life may be aggravated if an episode of asphyxia occurs during fetal life. The aim of the study was to examine regional cerebral blood flow (RCBF), histological changes, and cerebral brain metabolism in preterm lambs, and to analyze the role of oxidative stress in the first hours of postnatal life following severe fetal asphyxia. Eighteen chronically instrumented newborn lambs were randomly assigned to either a control group or the hypoxic-ischemic (HI) group, in which case fetal asphyxia was induced just before delivery. All the animals were maintained on intermittent positive pressure ventilation for 3 h after delivery. During the HI insult, the injured group developed acidosis, hypoxia, hypercapnia, lactic acidosis, and tachycardia (relative to the control group), without hypotension. The intermittent positive pressure ventilation transiently improved gas exchange and cardiovascular parameters. After HI injury and during ventilatory support, there continued to be an increased RCBF in inner regions among the HI group, but no significant differences were detected in cortical flow compared to the control group. Also, the magnitude of the increase in TUNEL positive cells (apoptosis) and antioxidant enzymes, and decrease of ATP reserves was significantly greater in the brain regions where the RCBF was not higher. In conclusion, our findings identify early metabolic, histological, and hemodynamic changes involved in brain damage in premature asphyxiated lambs. Such changes have been described in human neonates, so our model could be useful to test the safety and the effectiveness of different neuroprotective or ventilation strategies applied in the first hours after fetal HI injury.
authors.affiliation: Research Unit on Experimental Respiratory Physiology, Cruces Hospital Bizkaia, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rey-Santano
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Mielgo
authors.forename: Elena
authors.initials: E
authors.lastname: Gastiasoro
authors.forename: Xabier
authors.initials: X
authors.lastname: Murgia
authors.forename: Hector
authors.initials: H
authors.lastname: Lafuente
authors.forename: Estibaliz
authors.initials: E
authors.lastname: Ruiz-Del-Yerro
authors.forename: Adolf
authors.initials: A
authors.lastname: Valls-I-Soler
authors.forename: Enrique
authors.initials: E
authors.lastname: Hilario
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Alvarez