title: Investigating Mithramycin deoxysugar biosynthesis: enzymatic total synthesis of TDP-D-olivose.
abstract: Mix'n'match: Enzymatic total synthesis of TDP-D-olivose was achieved, starting from TDP-4-keto-6-deoxy-D-glucose, by combining three pathway enzymes with one cofactor-regenerating enzyme. The results also revealed that MtmC is a bifunctional enzyme that can perform a 4-ketoreduction necessary for D-olivose biosynthesis besides the previously found C-methyltransfer for D-mycarose biosynthesis.
authors.affiliation: Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY 40536-0596, USA.
authors.forename: Guojun
authors.initials: G
authors.lastname: Wang
authors.forename: Madan K
authors.initials: MK
authors.lastname: Kharel
authors.forename: Pallab
authors.initials: P
authors.lastname: Pahari
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
mesh_terms: D003837:Deoxy Sugars / Q000096:biosynthesis* / Q000737:chemistry; D004798:Enzymes / Q000378:metabolism; D005947:Glucose / Q000031:analogs & derivatives / Q000737:chemistry; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis* / Q000737:chemistry; D010084:Oxidation-Reduction; D008926:Plicamycin / Q000096:biosynthesis* / Q000737:chemistry; D013942:Thymine Nucleotides / Q000737:chemistry
chemical_list: D003837:Deoxy Sugars; D004798:Enzymes; D009702:Nucleoside Diphosphate Sugars; D013942:Thymine Nucleotides; C112447:dTDP-6-deoxy-4-ketoglucose; C407877:olivose; D005947:Glucose; D008926:Plicamycin