title: [An efficient and accurate method for counting target molecules in phage-display peptide library].
abstract: The phage titer of samples representing the low, intermediate and high phage number was respectively determined by the double-layer agar plate (DLAP) method and real-time PCR assay. The two methods accurately measured the titer of samples. The plaques from about 1/3 double-agar layer plates could be used to determine the phage titer. The DLAP experiment should repeat 10 times with 10 microl sample each time, while the within-assay coefficient variation (CV) was 4.93%-30.38%. At the same time, the real-time PCR assay only repeated 3 times with 1 microl phage each time, while CV for within-assay ranged from 0.02% to 0.25%. Results indicated that real-time PCR is a simple and quick method for determining bacteriophage titer.
authors.affiliation: Department of Parasitology, School of Medicine, University of South China, Hengyang 421001, China.
authors.forename: Ke-Geng
authors.initials: KG
authors.lastname: Wang
authors.forename: Qing-Ren
authors.initials: QR
authors.lastname: Zeng
authors.forename: Zheng-Yang
authors.initials: ZY
authors.lastname: Yu
authors.forename: Tie-Bing
authors.initials: TB
authors.lastname: Zeng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D019151:Peptide Library; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D019151:Peptide Library