title: Non-invasive monitoring of cytotoxicity based on kinetic changes of cellular autofluorescence.
abstract: A quantitative, non-destructive cellular autofluorescence based in vitro imaging assay has been developed and applied to study the cytotoxicity of Sodium Lauryl Sulfate (SLS) and HgCl2 on Balb/c 3T3 cells. A phenomenological double logistic model was proposed to quantify and relate the observed kinetic changes of fluorescence to the toxic potency of chemical compounds. This work forms the basis for cellular autofluorescence measurements in in vitro toxicity screening assays.
authors.affiliation: Institute for Health and Customer Protection, European Commission Joint Research Centre, 21-027 Ispra (VA), Italy. a.bednarkiewicz@int.pan.wroc.pl
authors.forename: Artur
authors.initials: A
authors.lastname: Bednarkiewicz
authors.forename: Robim M
authors.initials: RM
authors.lastname: Rodrigues
authors.forename: Maurice P
authors.initials: MP
authors.lastname: Whelan
mesh_terms: D000818:Animals; D041702:BALB 3T3 Cells; D001681:Biological Assay; D002470:Cell Survival; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005453:Fluorescence; D007700:Kinetics; D008627:Mercuric Chloride / Q000633:toxicity*; D051379:Mice; D009243:NAD / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013501:Surface-Active Agents / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D013501:Surface-Active Agents; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D012967:Sodium Dodecyl Sulfate; D008627:Mercuric Chloride