title: Tick cell lines for study of Crimean-Congo hemorrhagic fever virus and other arboviruses.
abstract: Continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. Their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. A preliminary study of propagation of the human pathogen Crimean-Congo hemorrhagic fever virus (CCHFV) in tick cell lines is reported; CCHFV replicated in seven cell lines derived from the ticks Hyalomma anatolicum (a known vector), Amblyomma variegatum, Rhipicephalus (Boophilus) decoloratus, Rhipicephalus (Boophilus) microplus, and Ixodes ricinus, but not in three cell lines derived from Rhipicephalus appendiculatus and Ornithodoros moubata. This indicates that tick cell lines can be used to study growth of CCHFV in arthropod cells and that there may be species-specific restriction in permissive CCHFV infection at the cellular level.
authors.affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom. l.sakyi@ed.ac.uk
authors.forename: Lesley
authors.initials: L
authors.lastname: Bell-Sakyi
authors.forename: Alain
authors.initials: A
authors.lastname: Kohl
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Bente
authors.forename: John K
authors.initials: JK
authors.lastname: Fazakerley
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000166:cytology / Q000821:virology*; D001102:Arbovirus Infections / Q000276:immunology / Q000635:transmission / Q000821:virology; D001103:Arboviruses / Q000254:growth & development* / Q000502:physiology; D002460:Cell Line; D003225:Hemorrhagic Fever Virus, Crimean-Congo / Q000254:growth & development* / Q000502:physiology; D006479:Hemorrhagic Fever, Crimean / Q000276:immunology / Q000635:transmission / Q000821:virology; D006801:Humans; D007113:Immunity, Innate; D026863:Ixodidae / Q000166:cytology / Q000821:virology*; D026861:Ornithodoros / Q000166:cytology / Q000821:virology*; D013045:Species Specificity; D017282:Tick-Borne Diseases / Q000276:immunology / Q000635:transmission / Q000821:virology; D010948:Viral Plaque Assay; D014779:Virus Replication