title: Genomic and physiological footprint of the Deepwater Horizon oil spill on resident marsh fishes.
abstract: The biological consequences of the Deepwater Horizon oil spill are unknown, especially for resident organisms. Here, we report results from a field study tracking the effects of contaminating oil across space and time in resident killifish during the first 4 mo of the spill event. Remote sensing and analytical chemistry identified exposures, which were linked to effects in fish characterized by genome expression and associated gill immunohistochemistry, despite very low concentrations of hydrocarbons remaining in water and tissues. Divergence in genome expression coincides with contaminating oil and is consistent with genome responses that are predictive of exposure to hydrocarbon-like chemicals and indicative of physiological and reproductive impairment. Oil-contaminated waters are also associated with aberrant protein expression in gill tissues of larval and adult fish. These data suggest that heavily weathered crude oil from the spill imparts significant biological impacts in sensitive Louisiana marshes, some of which remain for over 2 mo following initial exposures.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA. andreww@lsu.edu
authors.forename: Andrew
authors.initials: A
authors.lastname: Whitehead
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dubansky
authors.forename: Charlotte
authors.initials: C
authors.lastname: Bodinier
authors.forename: Tzintzuni I
authors.initials: TI
authors.lastname: Garcia
authors.forename: Scott
authors.initials: S
authors.lastname: Miles
authors.forename: Chet
authors.initials: C
authors.lastname: Pilley
authors.forename: Vandana
authors.initials: V
authors.lastname: Raghunathan
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Roach
authors.forename: Nan
authors.initials: N
authors.lastname: Walker
authors.forename: Ronald B
authors.initials: RB
authors.lastname: Walter
authors.forename: Charles D
authors.initials: CD
authors.lastname: Rice
authors.forename: Fernando
authors.initials: F
authors.lastname: Galvez
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D017753:Ecosystem; D054750:Ecotoxicology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D023781:Fundulidae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D060851:Gulf of America; D059392:Petroleum Pollution / Q000009:adverse effects* / Q000032:analysis; D043922:Toxicogenetics; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity
chemical_list: D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1