title: Selective capture of phosphopeptides by zirconium phosphonate-magnetic nanoparticles.
abstract: Immobilized metal affinity chromatography is a widely used method for the enrichment of phosphopeptides from proteolytic digests prior to mass spectrometric analysis. Here, we describe the selective enrichment of phosphopeptides from tryptic digests of proteins (α- and β-caseins) by zirconium phosphonate-magnetic Fe(3)O(4)/SiO(2) (core/shell) nanoparticles for phosphoproteome analysis with MALDI-TOF mass spectrometry.
authors.affiliation: National Chromatographic R & A Center, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Ren'an
authors.initials: R
authors.lastname: Wu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D063065:Organophosphonates / Q000737:chemistry*; D010748:Phosphopeptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles; D063065:Organophosphonates; D010748:Phosphopeptides; D011437:Propylamines; D012821:Silanes; D012997:Solvents; D012822:Silicon Dioxide; D015040:Zirconium; C477625:amino-propyl-triethoxysilane