title: Laser speckle contrast imaging of cerebral blood flow in freely moving animals.
abstract: We designed a miniature laser speckle imager that weighs ∼20 g and is 3.1-cm high for full-field high-resolution imaging of cerebral blood flow (CBF) in freely moving animals. Coherent laser light illuminates the cortex through a multimode optical fiber bundle fixed onto the supporting frame of the imager. The reflected lights are then collected by a miniature macrolens system and imaged by a high-resolution CMOS camera at a high frame rate (50 fps). Using this miniature imager, we achieve high spatiotemporal resolution laser speckle contrast imaging of CBF in freely moving animals in real time.
authors.affiliation: Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai, 200240, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.forename: Hongyang
authors.initials: H
authors.lastname: Lu
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.forename: Shanbao
authors.initials: S
authors.lastname: Tong
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D003952:Diagnostic Imaging / Q000379:methods*; D005336:Fiber Optic Technology; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008297:Male; D009068:Movement; D051381:Rats; D017207:Rats, Sprague-Dawley