title: A 10,000-fold nuclear hyperpolarization of a membrane protein in the liquid phase via a solid-state mechanism.
abstract: Several techniques rely on electron-nuclear interactions to boost the polarization of nuclear spins in the solid phase. Averaging out of anisotropic interactions as a result of molecular tumbling strongly reduces the applicability of such hyperpolarization approaches in liquids. Here we show for the first time that anisotropic electron-nuclear interactions in solution can survive sufficiently long to generate nuclear spin polarization by the solid-state photo-CIDNP mechanism. A 10,000-fold NMR signal increase in solution was observed for a giant biomolecular complex of a photosynthetic membrane protein with a tumbling correlation time in the submicrosecond regime, corresponding to a molecular weight close to 1 MDa.
authors.affiliation: Leiden Institute of Chemistry, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
authors.forename: Eugenio
authors.initials: E
authors.lastname: Daviso
authors.forename: Geertje Jacoba
authors.initials: GJ
authors.lastname: Janssen
authors.forename: A
authors.initials: A
authors.lastname: Alia
authors.forename: Gunnar
authors.initials: G
authors.lastname: Jeschke
authors.forename: Jörg
authors.initials: J
authors.lastname: Matysik
authors.forename: Marco
authors.initials: M
authors.lastname: Tessari
mesh_terms: D004123:Dimethylamines / Q000737:chemistry; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D012242:Rhodobacter sphaeroides / Q000737:chemistry
chemical_list: D004123:Dimethylamines; D008565:Membrane Proteins; C014518:dodecyldimethylamine oxide