title: [Role of protein kinases in prolactin signaling in bovine oocyte-cumulus complexes].
abstract: Mechanisms of prolactin signal transduction in generative and somatic cells of mammalian ovarian follicles have been studied only to a small extent. In the present work, the involvement oftyrosine kinases and protein kinase C in mediating of the previously revealed modulating effects of prolactin on the nuclear maturation of bovine oocytes and the morphologic-functional state of surrounding cumulus cells was investigated in vitro. Tyrosine kinase inhibitor, genistein, was found to suppress the stimulating action of prolactin on the completion of oocyte nuclear maturation and cumulus expansion, whereas protein kinase C inhibitor, calpostin C, did not affect these hormonal effects. Furthermore, both genistein and calpostin C inhibited the inducing influence of prolactin on the proliferative activity of cumulus cells. At the same time the retarding action ofprolactin on destructive processes in cumulus cells was blocked only in the presence of calpostin C. The results of the study suggest that the stimulatory influence of prolactin on oocyte nuclear maturation and attendant cumulus expansion is achieved with the participation of tyrosine kinases, whereas the modulating action of the hormone on the functional state of cumulus cells depends on activation of not only tyrosine kinases, but also protein kinase C.
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Lebedeva
authors.forename: G N
authors.initials: GN
authors.lastname: Singina
authors.forename: T E
authors.initials: TE
authors.lastname: Taradaĭnik
authors.forename: N A
authors.initials: NA
authors.lastname: Zinov'eva
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques; D003470:Culture Media; D054885:Cumulus Cells / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D019833:Genistein / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism* / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism / Q000502:physiology*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
chemical_list: D003470:Culture Media; D047428:Protein Kinase Inhibitors; D011388:Prolactin; D019833:Genistein; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C