title: Purification and characterization of a lectin with antiproliferative activity toward cancer cells from the dried fruit bodies of Lactarius flavidulus.
abstract: The isolation of a dimeric 29.8-kDa lectin (LFL) from dried Lactarius flavidulus fruit bodies is reported herein. The chromatographic procedure utilized comprised anion-exchange chromatography on DEAE-cellulose, cation-exchange chromatography on CM-cellulose, anion-exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The hemagglutinating activity of LFL was inhibited by a variety of simple sugars, such as lactose, p-nitrophenyl α-D-glucopyranoside, p-nitrophenyl β-D-glucopyranoside and inositol, and by the polysaccharide inulin. The activity of LFL was stable up to 40°C. There was a precipitous drop in activity when the temperature was elevated to 50°C. Hemagglutinating activity was retained when LFL was exposed to 6.25-12.5 mM HCl and NaOH. The activity was potently inhibited by Fe(2+) and Fe(3+) ions, and slightly inhibited by Al(3+) and Mn(2+) ions. LFL suppressed the proliferation of hepatoma (HepG2) and leukemic (L1210) cells with an IC(50) of 8.90 and 6.81 μM, respectively. It inhibited the activity of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC(50) of 5.68 μM. However, LFL did not exhibit antifungal activity.
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.forename: Hexiang
authors.initials: H
authors.lastname: Wang
authors.forename: Tzi Bun
authors.initials: TB
authors.lastname: Ng
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D004912:Erythrocytes / Q000187:drug effects; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011817:Rabbits; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D006388:Hemagglutinins; D037102:Lectins; D054303:HIV Reverse Transcriptase