title: A novel hemoglobin-based oxygen carrier, polymerized porcine hemoglobin, inhibits H₂O₂-induced cytotoxicity of endothelial cells.
abstract: Hemoglobin-based oxygen carriers (HBOCs), with their capacity for delivering oxygen, could potentially function as red blood cell substitutes or primary resuscitation solutions. However, there has been some concern regarding redox-related safety issues of HBOCs. The present study describes a novel function of polymerized porcine hemoglobin (pPolyHb) in protecting a human umbilical vein endothelial cell line from H₂O₂-induced cytotoxicity. Through the examination of H₂O₂ consumption and ferrylhemoglobin formation, we found that pPolyHb exhibits antioxidant activity, suggesting that pPolyHb may protect cells from free radical-induced cell damage. Additionally, we investigated the effect of pPolyHb on H₂O₂-induced cell cytotoxicity, and found that pPolyHb significantly inhibits H₂O₂-mediated endothelial cell damage as well as apoptosis. Thus, pPolyHb may be developed as a new HBOC in the future.
authors.affiliation: College of Life Science, Northwest University, Xi'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Kunping
authors.initials: K
authors.lastname: Yan
authors.forename: Penggao
authors.initials: P
authors.lastname: Dai
authors.forename: Jingjing
authors.initials: J
authors.lastname: Tian
authors.forename: Hongli
authors.initials: H
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001802:Blood Substitutes / Q000737:chemistry* / Q000494:pharmacology*; D003603:Cytotoxins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D058105:Polymerization; D013552:Swine
chemical_list: D001802:Blood Substitutes; D003603:Cytotoxins; D006861:Hydrogen Peroxide