title: Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA.
abstract: Several investigators have shown that CpG-DNA has outstanding effects as a Th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. In this study, we showed that encapsulation with phosphatidyl-Β-oleoyl-γ-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) complex enhances the immunostimulatory activity of CpG DNA and the binding of CpG-DNA to TLR9. We also examined involvement of myeloid differentiation protein (MyD88) and NF-κB activation in liposome-encapsulated CpG-DNA-induced IL-8 promoter activation. In this manuscript, the natural phosphodiester bond CpG-DNA encapsulated by DOPE : CHEMS complex is designated as Lipoplex(O). Importantly, we successfully screened B cell epitopes of envelope protein (E protein) of hepatitis C virus (HCV-E) and attachment glycoprotein G of human respiratory syncytial virus (HRSV-G) by immunization with complexes of several peptides and Lipoplex(O) without carriers. Therefore, Lipoplex(O) is potentially applicable as a universal adjuvant for peptide-based epitope screening and antibody production.
authors.affiliation: Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Dongbum
authors.initials: D
authors.lastname: Kim
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Younghee
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000276:Adjuvants, Immunologic; D000906:Antibodies / Q000276:immunology / Q000378:metabolism*; D002788:Cholesterol Esters / Q000737:chemistry; D018899:CpG Islands / Q000276:immunology*; D004247:DNA / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology*; D016174:Hepacivirus / Q000378:metabolism; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry*; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051217:Toll-Like Receptor 9 / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000276:Adjuvants, Immunologic; D000906:Antibodies; D002788:Cholesterol Esters; D000939:Epitopes; D016209:Interleukin-8; D008081:Liposomes; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D010455:Peptides; D010714:Phosphatidylethanolamines; D051217:Toll-Like Receptor 9; D014759:Viral Envelope Proteins; D004247:DNA; C013440:cholesteryl succinate