title: Effects of topical application of EGCG on testosterone-induced hair loss in a mouse model.
abstract: We investigated the effect of topical epigallocatechin-3-gallate (EGCG) on testosterone (T)-induced hair loss in mice. Marked hair loss was observed at the T-injected site, and topical EGCG significantly reduced the hair loss (P < 0.05). TUNEL staining showed apoptosis of follicular epithelial cells in the T-injected groups where topical EGCG was found to significantly diminish T-induced apoptosis (P < 0.05). Topical EGCG down-regulated the T-induced expression of androgen receptor but did not down-regulate 17β-hydroxysteroid dehydrogenase (HSD) and three β-HSD expression. Analysis using liquid chromatography tandem mass spectrometry (LC-MS/MS) on serum and tissue samples revealed no significant difference in T and dihydrotestosterone concentrations between the T-injected and T + EGCG groups. Thus, we found that T injection in a mouse model induces hair loss by apoptosis of the hair follicles rather than through the androgen metabolic pathway and also saw that T-induced apoptosis of hair follicles was reduced by topical EGCG.
authors.forename: Yoon Young
authors.initials: YY
authors.lastname: Kim
authors.forename: Sun
authors.initials: S
authors.lastname: Up No
authors.forename: Min Ho
authors.initials: MH
authors.lastname: Kim
authors.forename: Hei Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Hoon
authors.initials: H
authors.lastname: Kang
authors.forename: Hyung Ok
authors.initials: HO
authors.lastname: Kim
authors.forename: Young Min
authors.initials: YM
authors.lastname: Park
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000505:Alopecia / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017373:Cyproterone Acetate / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013196:Dihydrotestosterone / Q000378:metabolism; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006197:Hair / Q000187:drug effects / Q000378:metabolism; D018859:Hair Follicle / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D011944:Receptors, Androgen / Q000378:metabolism; D012627:Sebaceous Glands / Q000187:drug effects / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D013739:Testosterone; D017373:Cyproterone Acetate; D002392:Catechin; C045651:epigallocatechin gallate; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases