title: Increase of the activity of phase II antioxidant enzymes in rats after a single dose of coffee.
abstract: This study evaluated the acute effect of the administration of coffee brew in the activity of phase II antioxidant enzymes in the hepatic tissue of rats. A single dose of this beverage increased the activity of the enzymes SOD, CAT, and GPx; the maximum increase occurred 1 h after administration (19.1, 22.1, and 25.1%, respectively). These changes were statistically significant (p < 0.05), the response was shown to be dose-dependent (p < 0.05), and the return to basal levels took >4 h from the intervention, suggesting a long-term effect. The total antioxidant capacity of the hepatic tissue also exhibited a peak 1 h after the intervention (6.5%), but the increase was not statistically significant and the response was not dose-dependent due to the low exposure to coffee. These results indicate that coffee increases the activities of antioxidant enzymes, improving protection against oxidative stress.
authors.affiliation: Department of Nutrition, School of Public Health, University of São Paulo, São Paulo/SP, Brazil.
authors.forename: Silvio José Valadão
authors.initials: SJ
authors.lastname: Vicente
authors.forename: Emília Yasuko
authors.initials: EY
authors.lastname: Ishimoto
authors.forename: Robison José
authors.initials: RJ
authors.lastname: Cruz
authors.forename: Camilo Dias Seabra
authors.initials: CD
authors.lastname: Pereira
authors.forename: Elizabeth Aparecida Ferraz Da Silva
authors.initials: EA
authors.lastname: Torres
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism*; D002934:Cinnamates / Q000032:analysis; D003069:Coffee / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D003069:Coffee; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase