title: Identification and detection method for genetically modified papaya resistant to papaya ringspot virus YK strain.
abstract: Unauthorized genetically modified (GM) papaya (Carica papaya LINNAEUS) was detected in a commercially processed product, which included papaya as a major ingredient, in Japan. We identified the transgenic vector construct generated based on resistance to infection with the papaya ringspot virus (PRSV) YK strain. A specific detection method to qualitatively monitor papaya products for contamination with the GM papaya was developed using the real-time polymerase chain reaction.
authors.affiliation: National Institute of Health Science, Tokyo, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Ohmori
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Reona
authors.initials: R
authors.lastname: Takabatake
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kondo
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
mesh_terms: D029441:Carica / Q000235:genetics / Q000276:immunology / Q000821:virology*; D004247:DNA / Q000032:analysis; D005506:Food Contamination; D007564:Japan; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014780:Viruses / Q000276:immunology
chemical_list: D004247:DNA