title: A flow-through microarray cell for the online SERS detection of antibody-captured E. coli bacteria.
abstract: We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 10(3) to 4.3 × 10(5) cells/mL. The method has potential for routine application, e.g. for drinking water control.
authors.affiliation: Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, 81377 Munich, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Knauer
authors.forename: Natalia P
authors.initials: NP
authors.lastname: Ivleva
authors.forename: Reinhard
authors.initials: R
authors.lastname: Niessner
authors.forename: Christoph
authors.initials: C
authors.lastname: Haisch
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D009862:Online Systems; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized