title: Molecular epidemiology of human herpesvirus 8 variants in Kaposi's sarcoma from Iranian patients.
abstract: Kaposi's sarcoma (KS) is a rare cancer in Iran and there is no epidemiological and molecular information about HHV-8 variants circulating among the Iranian population. In this study HHV-8 sequences have been analyzed in 43 cutaneous KS biopsies from Iranian patients mainly affected by classic KS. DNA samples were subjected to PCR amplification of HHV-8 ORF26, T0.7 and K1 followed by direct nucleotide sequencing and phylogenetic analysis. The analysis of ORF26 showed that 30 (69.8%) and 13 (30.2%) samples belonged to subtypes A/C and K, respectively. In general, the clustering of HHV-8 T0.7 variants paralleled that of ORF26. Genotyping of K1 sequences showed that the majority of samples (39 out of 41) fall into the large C clade with only 2 belonging to the A clade. In conclusion, HHV-8 variants identified among classic Iranian KS are largely related to Eurasian genotypes previously identified in KS from Mediterranean, Middle East, and East Asian regions.
authors.affiliation: School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Jalilvand
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Tornesello
authors.forename: Franco M
authors.initials: FM
authors.lastname: Buonaguro
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
authors.forename: Zahra Safaie
authors.initials: ZS
authors.lastname: Naraghi
authors.forename: Zabihollah
authors.initials: Z
authors.lastname: Shoja
authors.forename: Abed-Ali
authors.initials: AA
authors.lastname: Ziaee
authors.forename: Rasool
authors.initials: R
authors.lastname: Hamkar
authors.forename: Shohreh
authors.initials: S
authors.lastname: Shahmahmoodi
authors.forename: Rakhshandeh
authors.initials: R
authors.lastname: Nategh
authors.forename: Talat
authors.initials: T
authors.lastname: Mokhtari-Azad
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005838:Genotype; D019288:Herpesvirus 8, Human / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012514:Sarcoma, Kaposi / Q000453:epidemiology* / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D012867:Skin / Q000821:virology
chemical_list: D004279:DNA, Viral