title: Altered PLCβ-1 expression in the gerbil hippocampal complex following spontaneous seizure.
abstract: Although the phospholipase C (PLC)β-1 isoform is associated with spontaneous seizure and distinctively expressed in the telencephalon, the distribution of PLCβ-1 expression in the epileptic gerbil hippocampus remains controversial. Therefore, we determined whether PLCβ-1 is associated with spontaneous seizure in an animal model of genetic epilepsy. In the present study, PLCβ-1 immunoreactivity was down-regulated in seizure-sensitive (SS) gerbils more than in seizure-resistant (SR) gerbils. The expression of PLCβ-1 within calretinin (CR)- positive neurons was rarely detected within the dentate hilar region of SS gerbils. PLCβ-1 immunoreactivity in the hippocampus was significantly elevated as compared to that in pre-seizure SS gerbil 3 h post-ictal. These findings suggest that alterations in PLCβ-1 immunoreactivity in the SS gerbil hippocampus may be closely related to the epileptic state of the gerbil brain and transiently elevated PLCβ-1 protein levels following seizure episodes. Such alterations may be compensatory responses in the SS gerbil hippocampus.
authors.affiliation: Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan 330-090, Korea.
authors.forename: Saet-Byeol
authors.initials: SB
authors.lastname: Lee
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Oh
authors.forename: Jae-Kwang
authors.initials: JK
authors.lastname: Chung
authors.forename: Ji-Heon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Sang-Duk
authors.initials: SD
authors.lastname: Lee
authors.forename: Dae-Kyoon
authors.initials: DK
authors.lastname: Park
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Park
authors.forename: Jeong-Sik
authors.initials: JS
authors.lastname: Ko
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000818:Animals; D064032:Calbindin 2; D004195:Disease Models, Animal; D015536:Down-Regulation; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism*; D054799:Phospholipase C beta / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012640:Seizures / Q000378:metabolism / Q000473:pathology*
chemical_list: D064032:Calbindin 2; D064030:S100 Calcium Binding Protein G; D054799:Phospholipase C beta