title: Biodiesel synthesis and conformation of lipase from Burkholderia cepacia in room temperature ionic liquids and organic solvents.
abstract: Biodiesel synthesis and conformation of Burkholderia cepacia lipase (BCL) were studied in 19 different room temperature ionic liquids (RTLLs) with a range of cation and anion structures. Overall, anion selection had a greater influence on biodiesel conversion than cation choice. RTILs containing Tf2N- and PF6- anions were suitable reaction media, while RTIL of [OmPy][BF4] was the best reaction medium with a biodiesel yield of 82.2±1.2%. RTILs with strong water miscible properties showed very low biodiesel yields. Conformational analysis by FT-IR revealed that higher biodiesel conversion in RTILs was correlated with a low tendency in α-helix content of BCL. An ultrasound-assisted biocatalysis process in RTILs was used to improve mass transfer rate, leading to 83% reduction of the reaction time for biodiesel production.
authors.affiliation: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China. liuyunprivate@sina.com
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.forename: Yunjun
authors.initials: Y
authors.lastname: Yan
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D000838:Anions; D055162:Biocatalysis; D056804:Biofuels / Q000032:analysis*; D016956:Burkholderia cepacia / Q000201:enzymology*; D002412:Cations; D004951:Esterification; D052578:Ionic Liquids / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry* / Q000378:metabolism*; D009930:Organic Chemicals / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014465:Ultrasonics
chemical_list: D000838:Anions; D056804:Biofuels; D002412:Cations; D052578:Ionic Liquids; D009930:Organic Chemicals; D008049:Lipase