title: Multienzymatic sucrose conversion into fructose and gluconic acid through fed-batch and membrane-continuous processes.
abstract: Multienzymatic conversion of sucrose into fructose and gluconic acid was studied through fed-batch and continuous (in a membrane reactor) processes. The law of substrate addition (sucrose or glucose) for the fed-batch process which led to a yield superior to 80% was the decreasing linear type, whose feeding rate (ϕ; L/h) was calculated through the equation: ϕ = ϕ(o) - k.t, where ϕ(o) (initial feeding rate, L/h), k (linear addition constant, L/h (2)), and t (reaction time, h). In the continuous process, the yield of conversion of sucrose (Y) was superior to 70% under the following conditions: dilution rate = 0.33 h(-1), total duration of 15 h, pH 5.0, 37 °C and initial sucrose concentration of 64 g/L (Y = 92%), 100 g/L (Y = 83%), or 150 g/L (Y = 76%).
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences, Av. Prof. Lineu Prestes, 580, B.16, 05508-900 São Paulo, SP, Brazil. fadijr@usp.br
authors.forename: Fadi Antoine
authors.initials: FA
authors.lastname: Taraboulsi
authors.forename: Ester Junko
authors.initials: EJ
authors.lastname: Tomotani
authors.forename: Michele
authors.initials: M
authors.lastname: Vitolo
mesh_terms: D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology*; D001709:Biotechnology / Q000379:methods*; D002374:Catalase / Q000737:chemistry*; D002417:Cattle; D005632:Fructose / Q000737:chemistry*; D005942:Gluconates / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D013395:Sucrose / Q000737:chemistry; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D005942:Gluconates; D029701:Saccharomyces cerevisiae Proteins; D005632:Fructose; D013395:Sucrose; D005949:Glucose Oxidase; D002374:Catalase; D043324:beta-Fructofuranosidase; C030691:gluconic acid