title: Characterization of metabolite quantitative trait loci and metabolic networks that control glucosinolate concentration in the seeds and leaves of Brassica napus.
abstract: • Glucosinolates are a major class of secondary metabolites found in the Brassicaceae, whose degradation products are proving to be increasingly important for human health and in crop protection. • The genetic and metabolic basis of glucosinolate accumulation was dissected through analysis of total glucosinolate concentration and its individual components in both leaves and seeds of a doubled-haploid (DH) mapping population of oilseed rape/canola (Brassica napus). • The quantitative trait loci (QTL) that had an effect on glucosinolate concentration in either or both of the organs were integrated, resulting in 105 metabolite QTL (mQTL). Pairwise correlations between individual glucosinolates and prior knowledge of the metabolic pathways involved in the biosynthesis of different glucosinolates allowed us to predict the function of genes underlying the mQTL. Moreover, this information allowed us to construct an advanced metabolic network and associated epistatic interactions responsible for the glucosinolate composition in both leaves and seeds of B. napus. • A number of previously unknown potential regulatory relationships involved in glucosinolate synthesis were identified and this study illustrates how genetic variation can affect a biochemical pathway.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Feng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Life Sciences, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK.
authors.forename: Guy
authors.initials: G
authors.lastname: Barker
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Meng
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology; D004843:Epistasis, Genetic; D017343:Genes, Plant / Q000235:genetics; D008040:Genetic Linkage; D005961:Glucosinolates / Q000378:metabolism*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D005961:Glucosinolates