title: High-speed multineuron calcium imaging using Nipkow-type confocal microscopy.
abstract: Conventional confocal and two-photon microscopy scan the field of view sequentially with single-point laser illumination. This raster-scanning method constrains video speeds to tens of frames per second, which are too slow to capture the temporal patterns of fast electrical events initiated by neurons. Nipkow-type spinning-disk confocal microscopy resolves this problem by the use of multiple laser beams. We describe experimental procedures for functional multineuron calcium imaging (fMCI) based on Nipkow-disk confocal microscopy, which enables us to monitor the activities of hundreds of neurons en masse at a cellular resolution at up to 2000 fps.
authors.affiliation: Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Takahashi
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Oba
authors.forename: Naoto
authors.initials: N
authors.lastname: Yukinawa
authors.forename: Sakiko
authors.initials: S
authors.lastname: Ujita
authors.forename: Mika
authors.initials: M
authors.lastname: Mizunuma
authors.forename: Norio
authors.initials: N
authors.lastname: Matsuki
authors.forename: Shin
authors.initials: S
authors.lastname: Ishii
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ikegaya
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods*; D009474:Neurons / Q000378:metabolism*
chemical_list: D002118:Calcium