title: Implementing photodissociation in an Orbitrap mass spectrometer.
abstract: We modified a dual pressure linear ion trap Orbitrap to permit infrared multiphoton dissociation (IRMPD) in the higher energy collisional dissociation (HCD) cell for high resolution analysis. A number of parameters, including the pressures of the C-trap and HCD cell, the radio frequency (rf) amplitude applied to the C-trap, and the HCD DC offset, were evaluated to optimize IRMPD efficiency and maintain a high signal-to-noise ratio. IRMPD was utilized for characterization of phosphopeptides, supercharged peptides, and N-terminal modified peptides, as well as for top-down protein analysis. The high resolution and high mass accuracy capabilities of the Orbitrap analyzer facilitated confident assignment of product ions arising from IRMPD.
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Vasicek
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Ledvina
authors.forename: Jared
authors.initials: J
authors.lastname: Shaw
authors.forename: Jens
authors.initials: J
authors.lastname: Griep-Raming
authors.forename: Michael S
authors.initials: MS
authors.lastname: Westphall
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Coon
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Brodbelt
mesh_terms: D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D055668:Photochemical Processes; D040901:Proteomics / Q000295:instrumentation / Q000379:methods
chemical_list: D010455:Peptides