title: [Heat shock protein 90--modulator of TNFalpha-induced apoptosis of Jurkat tumor cells].
abstract: rTNFalpha-induced programmed death of Jurkat tumor cells cultured with 17-AAG, a selective inhibitor of heat shock protein (Hsp90), was studied by fluorescent microscopy with the use of FITC-labeled annexin V and propidium iodide. Caspase-3 and -8 activities were determined by spectrophotometry using a caspase- 3 and -8 colorimetric assay kit. It was shown that inhibition of Hsp90 leads to activation of Jurkat cell apoptosis while Hsp90 itself suppresses this process. 17-AAG enhances rTNFa-induced apoptosis of tumor cells.
authors.forename: E V
authors.initials: EV
authors.lastname: Kaĭgorodova
authors.forename: N V
authors.initials: NV
authors.lastname: Riazantseva
authors.forename: V V
authors.initials: VV
authors.lastname: Novitskiĭ
authors.forename: A N
authors.initials: AN
authors.lastname: Moroshkina
authors.forename: M V
authors.initials: MV
authors.lastname: Belkina
authors.forename: V D
authors.initials: VD
authors.lastname: Iakushina
mesh_terms: D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D016227:Benzoquinones / Q000378:metabolism*; D053148:Caspase 3 / Q000032:analysis / Q000378:metabolism; D053181:Caspase 8 / Q000032:analysis / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D047029:Lactams, Macrocyclic / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D011419:Propidium; D013053:Spectrophotometry; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D016227:Benzoquinones; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D014409:Tumor Necrosis Factor-alpha; D011419:Propidium; C112765:tanespimycin; D053148:Caspase 3; D053181:Caspase 8; D016650:Fluorescein-5-isothiocyanate