title: Taming influenza viruses.
abstract: Plasmid-based reverse genetics systems allow the artificial generation of viruses with cloned cDNA-derived genomes. Since the establishment of such systems for influenza virus, numerous attempts have been made to tame this pathogenic agent. In particular, several types of viruses expressing foreign genes have been generated and used to further our knowledge of influenza virus replication and pathogenicity and to develop novel influenza vaccines. Here, we review these achievements and discuss future perspectives.
authors.affiliation: Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo, Japan. mozawa@vetmed.wisc.edu
authors.forename: Makoto
authors.initials: M
authors.lastname: Ozawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000096:biosynthesis; D007251:Influenza, Human / Q000276:immunology / Q000517:prevention & control* / Q000821:virology*; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis*; D059386:Reverse Genetics / Q000379:methods*; D019076:Transgenes; D014613:Vaccines, Attenuated / Q000008:administration & dosage* / Q000096:biosynthesis; D014779:Virus Replication / Q000235:genetics / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D007252:Influenza Vaccines; D012367:RNA, Viral; D014613:Vaccines, Attenuated