title: Cell-printing and transfer technology applications for bone defects in mice.
abstract: Bone regeneration therapy based on the delivery of osteogenic factors and/or cells has received a lot of attention in recent years since the discovery of pluripotent stem cells. We reported previously that the implantation of capillary networks engineered ex vivo by the use of cell-printing technology could improve blood perfusion. Here, we developed a new substrate prepared by coating glass with polyethylene glycol (PEG) to create a non-adhesive surface and subsequent photo-lithography to finely tune the adhesive property for efficient cell transfer. We examined the cell-transfer efficiency onto amniotic membrane and bone regenerative efficiency in murine calvarial bone defect. Cell transfer of KUSA-A1 cells (murine osteoblasts) to amniotic membrane was performed for 1 h using the substrates. Cell transfer using the substrate facilitated cell engraftment onto the amniotic membrane compared to that by direct cell inoculation. KUSA-A1 cells transferred onto the amniotic membrane were applied to critical-sized calvarial bone defects in mice. Micro-computed tomography (micro-CT) analysis showed rapid and effective bone formation by the cell-equipped amniotic membrane. These results indicate that the cell-printing and transfer technology used to create the cell-equipped amniotic membrane was beneficial for the cell delivery system. Our findings support the development of a biologically stable and effective bone regeneration therapy.
authors.affiliation: Department of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Tsugawa
authors.forename: Motohiro
authors.initials: M
authors.lastname: Komaki
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshida
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Nakahama
authors.forename: Teruo
authors.initials: T
authors.lastname: Amagasa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
mesh_terms: D000650:Amnion / Q000166:cytology / Q000637:transplantation; D000818:Animals; D001842:Bone and Bones / Q000473:pathology*; D002448:Cell Adhesion; D002452:Cell Count; D002460:Cell Line; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009929:Organ Size; D010006:Osteoblasts / Q000166:cytology* / Q000637:transplantation*; D010012:Osteogenesis; D012886:Skull / Q000000981:diagnostic imaging / Q000473:pathology; D023822:Tissue Engineering / Q000379:methods*; D055114:X-Ray Microtomography