title: Serum levels of IL-33 is increased in patients with ankylosing spondylitis.
abstract: Interleukin-33 (IL-33) is implicated in rheumatoid arthritis with effects of promoting tumor necrosis factor-alpha (TNF-α) and interleukin-17 (IL-17) productions, which have been demonstrated to play a pivotal role in ankylosing spondylitis (AS). However, changes of IL-33 levels and its effects in AS have not been investigated. Eighty-nine and 178 healthy controls were included in the current study. Erythrocyte sedimentation rate, serum levels of C-reactive protein, IL-17, and IL-33 were determined. Effects of IL-33 on TNF-α and IL-6 productions were investigated. Effects of IL-33 on neutrophil migration were also evaluated. Serum levels of IL-33 were elevated in AS patients. Moreover, IL-33 was significantly higher in active AS patients according to Bath Ankylosing Spondylitis Disease Activity Index. IL-33 concentrations in serum were positively correlated with TNF-α and IL-17 levels (IL-33 and TNF-α, r = 0.54, P < 0.01; IL-33 and IL-17, r = 0.47, P < 0.01). IL-33 dose-dependently enhanced TNF-α and IL-6 productions by peripheral blood mononuclear cells (PBMCs) responding to lipopolysaccharide. IL-33 induced neutrophil migration only in higher doses (≥10 ng/ml). Serum levels of IL-33 were elevated in AS patients. IL-33 may play a role in AS development via enhancing TNF-α production by PBMCs and inducing neutrophil migration.
authors.affiliation: Department of Spinal Surgery, Huangpu Division of First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Han
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Zeng
authors.forename: Chun-Xiang
authors.initials: CX
authors.lastname: Liang
authors.forename: Bai-Ling
authors.initials: BL
authors.lastname: Cheng
authors.forename: Bing-Sheng
authors.initials: BS
authors.lastname: Yu
authors.forename: Hao-Miao
authors.initials: HM
authors.lastname: Li
authors.forename: Fang Fang
authors.initials: FF
authors.lastname: Zeng
authors.forename: Shao-Yu
authors.initials: SY
authors.lastname: Liu
mesh_terms: D000328:Adult; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002465:Cell Movement; D005260:Female; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D000067596:Interleukin-33; D007378:Interleukins / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D013167:Spondylitis, Ankylosing / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: C506536:IL33 protein, human; D020381:Interleukin-17; D000067596:Interleukin-33; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D002097:C-Reactive Protein