title: Acute pulmonary injury induced by experimental muscle trauma.
abstract: OBJECTIVE
  To develop an easily reproducible model of acute lung injury due to experimental muscle trauma in healthy rats.
  
  
  METHODS
  Eighteen adult Wistar rats were randomized in 3 groups (n=6): G-1- control, G-2 - saline+trauma and G-3 - dexamethasone+trauma. Groups G-1 and G-2 were treated with saline 2,0 ml i.p; G-3 rats were treated with dexamethasone (DE) (2 mg/kg body weight i.p.). Saline and DE were applied 2h before trauma and 12h later. Trauma was induced in G-2 and G-3 anesthetized (tribromoethanol 97% 100 ml/kg i.p.) rats by sharp section of anterior thigh muscles just above the knee, preserving major vessels and nerves. Tissue samples (lung) were collected for myeloperoxidase (MPO) assay and histopathological evaluation.
  
  
  RESULTS
  Twenty-four hours after muscle injury there was a significant increase in lung neutrophil infiltration, myeloperoxidase activity and edema, all reversed by dexamethasone in G-3.
  
  
  CONCLUSIONS
  Trauma by severance of thigh muscles in healthy rats is a simple and efficient model to induce distant lung lesions.
authors.affiliation: Department of Surgery, Federal University of Ceara, Fortaleza-CE, Brazil.
authors.forename: Márcia Andréa da Silva Carvalho
authors.initials: MA
authors.lastname: Sombra
authors.forename: Marcelo Pinho Pessoa de
authors.initials: MP
authors.lastname: Vasconcelos
authors.forename: Sergio Botelho
authors.initials: SB
authors.lastname: Guimarães
authors.forename: Rodrigo Dornfeld
authors.initials: RD
authors.lastname: Escalante
authors.forename: José Huygens Parente
authors.initials: JH
authors.lastname: Garcia
authors.forename: Paulo Roberto Leitão de
authors.initials: PR
authors.lastname: Vasconcelos
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy / Q000209:etiology* / Q000473:pathology; D000818:Animals; D002452:Cell Count; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D005938:Glucocorticoids / Q000627:therapeutic use; D008168:Lung / Q000473:pathology*; D018482:Muscle, Skeletal / Q000293:injuries*; D020556:Neutrophil Infiltration / Q000502:physiology; D009504:Neutrophils / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013848:Thigh; D013997:Time Factors
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone; D009195:Peroxidase