title: Effects of ischemic preconditioning associated to different preservation solutions in protecting the intestinal graft.
abstract: OBJECTIVE
  To evaluate the effects of ischemic preconditioning (IPC) associate with different preservation solutions, in the protecting of gut.
  
  
  METHODS
  Four groups of 14 rats underwent laparotomy and collecting 20 cm of ileum, for preservation, at 4ºC, in Belzer (Belz), Ringer (RL), Celsior (Cs) and Custodiol (Cust) solutions, for 24 hours. Prior to collection, half of the animals in each group were subjected to IPC. During preservation, in the periods of zero, 12, 18 and 24 hours, were conducted evaluating the degree of mucosal injury and dosage of malondialdehyde acid (MDA).
  
  
  RESULTS
  In all periods the RL group, with and without IPC, presented MDA values higher than the Belz and Cs. The degree of mucosal injury in the non-ipc RLgroup with 12h preservation was higher than the others; with 18 and 24h, the RL and Cust had higher degrees of damage than Cs and Belz. With IPC, in all periods, the group Cs and Belz had lower degrees of injury.
  
  
  CONCLUSIONS
  The Celsior and Belzer solutions had better protective effects on the gut and these effects were enhanced by IPC.
authors.affiliation: Department of Surgery, University of Mato Grosso, Brazil. jneves@terra.com.br
authors.forename: José de Souza
authors.initials: Jde S
authors.lastname: Neves
authors.forename: Marcos de Souza
authors.initials: Mde S
authors.lastname: Abrahão
authors.forename: Alcides Augusto
authors.initials: AA
authors.lastname: Salzedas Netto
authors.forename: Edna Frasson de Souza
authors.initials: EF
authors.lastname: Montero
authors.forename: Adriano Miziara
authors.initials: AM
authors.lastname: Gonzalez
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D004187:Disaccharides / Q000494:pharmacology; D004573:Electrolytes / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007413:Intestinal Mucosa; D007421:Intestine, Small / Q000098:blood supply*; D019194:Ischemic Preconditioning; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008353:Mannitol / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000077331:Ringer's Solution; D013997:Time Factors
chemical_list: C039984:Bretschneider cardioplegic solution; C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D008353:Mannitol; D006639:Histidine; D008315:Malondialdehyde; D011343:Procaine; D000493:Allopurinol; D011189:Potassium Chloride; D000077331:Ringer's Solution; D005978:Glutathione; D005947:Glucose; D000241:Adenosine; D011887:Raffinose