title: [Therapeutic effects of bone marrow transplantation on ovarian injury in mice].
abstract: OBJECTIVE
  To evaluate the therapeutic effects of bone marrow transplantation (BMT) on ovarian injury induced by chemotherapy in mice.
  
  
  METHODS
  Forty-eight mice were randomized equally into normal control group (A), cyclophosphamide and BMT group (B), and cyclophosphamide group (C). The mice in groups B and C were treated with intraperitoneal injection of cyclophosphamide at the daily dose of 150 mg/kg for 3 consecutive days, and allogeneic bone marrow cell transplantation was performed in group B. The ovary coefficient and the amount of follicles were compared to evaluate the function of ovaries. For cell tracking, the bone marrow cells were labeled with Hoechst 33342 and detected through fluorescence microscope after transplantation.
  
  
  RESULTS
  On days 21 and 50 after cyclophosphamide treatment, the ovary coefficient and the amount of follicles were significantly lowered in groups B and C (P<0.05), but the reduction was obviously ameliorated in group B (P<0.05). Cell tracking showed the presence of the donor bone marrow cells in the ovaries of the recipients mice after BMT.
  
  
  CONCLUSIONS
  BMT can improve the ovarian function impaired by chemotherapy in mice.
authors.affiliation: Infertility Center, Qilu Hospital, Shandong University, Ji nan, China. llx0110@126.com
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Liang
authors.forename: Lan
authors.initials: L
authors.lastname: Chao
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Deng
authors.forename: Hong-ling
authors.initials: HL
authors.lastname: Yu
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Liu
authors.forename: Xu-ping
authors.initials: XP
authors.lastname: Wang
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D003520:Cyclophosphamide / Q000009:adverse effects*; D005260:Female; D051379:Mice; D010053:Ovary / Q000473:pathology / Q000503:physiopathology*
chemical_list: D003520:Cyclophosphamide