title: Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag-induced reporter fluorescence.
abstract: An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.
authors.affiliation: Lehrbereich Mikrobielle Genetik, Waldhäuser Str. 70/8, Eberhard Karls Universität Tübingen, Germany.
authors.forename: Tina
authors.initials: T
authors.lastname: Gauger
authors.forename: Felix
authors.initials: F
authors.lastname: Weihs
authors.forename: Sonja
authors.initials: S
authors.lastname: Mayer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Krismer
authors.forename: Jan
authors.initials: J
authors.lastname: Liese
authors.forename: Melanie
authors.initials: M
authors.lastname: Kull
authors.forename: Ralph
authors.initials: R
authors.lastname: Bertram
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D013752:Tetracycline