title: Size separation of proteins by capillary zone electrophoresis with cationic hitchhiking.
abstract: The paper describes a method of size separation of proteins by capillary sieving electrophoresis with cationic surfactant. Proteins are separated within 12 min with repeatability of migration times better than 0.2%. Some proteins achieve the separation efficiency of 200,000 theoretical plates. The method can be used for determination of protein relative molecular masses. The accuracy of the determined relative molecular masses and the limitation of the method were investigated by the analysis of more than 60 proteins. The method also allows separation of protein oligomers. Proteins can be quantitated after the electrokinetic injection in the concentration range 0.07-0.43 g/L. The average detection limit is about 2 mg/L.
authors.affiliation: Alcor BioSeparations LLC, Palo Alto, CA, USA. ladia@dolnik.net
authors.forename: Vladislav
authors.initials: V
authors.lastname: Dolnik
authors.forename: William A
authors.initials: WA
authors.lastname: Gurske
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D002412:Cations; D011506:Proteins; D012967:Sodium Dodecyl Sulfate