title: Diosmin ameliorates intestinal injury induced by hepatic ischemia reperfusion in rats.
abstract: BACKGROUND
  Hepatic ischemia-reperfusion causes histologic injury to the intestinal mucosa. We investigated the effects of diosmin, a phelobotrophic drug with antioxidant and antiinflammatory effects, on intestinal injury in the experimental liver ischemia-reperfusion model.
  
  
  METHODS
  Fourty rats were divided into four groups: sham group (Group 1), control group (Group 2), perop diosmin group (50 mg/kg) treatment group (Group 3) and preop 10-day diosmin (50 mg/kg) treatment group (Group 4). Ischemia-reperfusion model was carried out by clamping the hepatic pedicle for 60 min and then reperfusing the liver for 90 min. At the end of procedures, blood and ileum tissue samples were obtained for biochemical and histopathological assessments.
  
  
  RESULTS
  According to the results of liver function tests (AST, ALT and LDH) there was a significant difference between the control and other groups (p < 0.001 for all). According to the plasma and ileum oxidative stress parameters (MDA, GSH-Px and XO), there was a significant difference between the control and other groups (p < 0.05 for all). Histopathologically; the specimens in Group 2 showed specific morphological abnormalities (the epithelial lining of the apical surface of villi was degenerated and desquamated to the lumen). Group 3 and 4 showed ileal histomorphology similar to the sham group. Pathological scores were significantly different between Group 2 and other groups.
  
  
  CONCLUSIONS
  Diosmin can be administered for protection from destructive effects of hepatic ischemia-reperfusion injury on intestine in both emergent and elective hepatic surgical operations in which the possible ischemic periods are expected (Tab. 4, Fig. 1, Ref. 39).
authors.affiliation: Ankara Training and Research Hospital Department of 4th General Surgery, Ankara, Turkey. drtanrikulu@hotmail.com
authors.forename: Y
authors.initials: Y
authors.lastname: Tanrikulu
authors.forename: K
authors.initials: K
authors.lastname: Kismet
authors.forename: S
authors.initials: S
authors.lastname: Serin Kilicoglu
authors.forename: E
authors.initials: E
authors.lastname: Devrim
authors.forename: S
authors.initials: S
authors.lastname: Erel
authors.forename: C
authors.initials: C
authors.lastname: Sen Tanrikulu
authors.forename: S
authors.initials: S
authors.lastname: Dinc
authors.forename: O H
authors.initials: OH
authors.lastname: Edebal
authors.forename: E
authors.initials: E
authors.lastname: Erdemli
authors.forename: M A
authors.initials: MA
authors.lastname: Akkus
mesh_terms: D000818:Animals; D004145:Diosmin / Q000627:therapeutic use*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D007082:Ileum / Q000378:metabolism / Q000473:pathology*; D008099:Liver / Q000098:blood supply*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D004145:Diosmin; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase