title: Magnolol-loaded core-shell hydrogel nanoparticles: drug release, intracellular uptake, and controlled cytotoxicity for the inhibition of migration of vascular smooth muscle cells.
abstract: Encapsulation and release behavior of a water-insoluble drug, magnolol, using a core-shell polysaccharide-based nanoparticle, manipulating the cellular internalization and controlled cytotoxic effect of magnolol-loaded nanoparticles over the A10 vascular smooth muscle cells (VSMCs) was reported. A magnolol-polyvinylpyrrolidone (PVP) core phase was prepared, followed encapsulating by an amphiphilic carboxymethyl-hexanoyl chitosan (CHC) shell to form a magnolol-loaded core-shell hydrogel nanoparticles (termed magnolol-CHC nanoparticles). The resulting magnolol-CHC nanoparticles were employed for evaluation of drug release and controlled cytotoxic inhibition of VSMCs migration in vitro. A sustained release of the magnolol from the nanoparticles was determined. The magnolol-CHC nanoparticles exhibited outstanding cellular uptake efficiency, and under a cytotoxic evaluation, an increased antiproliferative effect and effective inhibition of VSMC migration as a result of efficient intracellular delivery of the encapsulated magnolol in comparison to free magnolol was achieved. We then envision a potential intracellular medication strategy with improved biological and therapeutic efficacy using the magnolol-CHC nanoparticles illustrated in this work.
authors.affiliation: Nano-Bioengineering Laboratory, Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu 300, Taiwan.
authors.forename: Yen-Jen
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yin-Chih
authors.initials: YC
authors.lastname: Chien
authors.forename: Chieh-Hsi
authors.initials: CH
authors.lastname: Wu
authors.forename: Dean-Mo
authors.initials: DM
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000493:pharmacokinetics / Q000494:pharmacology*; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D017705:Lignans / Q000493:pharmacokinetics / Q000494:pharmacology*; D018613:Microscopy, Confocal; D032389:Myocytes, Smooth Muscle / Q000187:drug effects*; D053758:Nanoparticles / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D003692:Delayed-Action Preparations; D017705:Lignans; C005498:magnolol; D014867:Water; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate