title: Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering.
abstract: A scaffold harboring the desired features such as biodegradation, biocompatibility, porous structure could serve as template for bone tissue engineering. In the present study, chitosan (CS), nano-scaled silicon dioxide (Si) and zirconia (Zr) were combined by freeze drying technique to fabricate a bio-composite scaffold. The bio-composite scaffold (CS/Si/Zr) was characterized by SEM, XRD and FT-IR studies. The scaffold possessed a porous nature with pore dimensions suitable for cell infiltration and colonization. The presence of zirconia in the CS/Si/Zr scaffold decreased swelling and increased biodegradation, protein adsorption and bio-mineralization properties. The CS/Si/Zr scaffold was also found to be non-toxic to rat osteoprogenitor cells. Thus, we suggest that CS/Si/Zr bio-composite scaffold is a potential candidate to be used for bone tissue engineering.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur-603 203, Tamilnadu, India.
authors.forename: Soumitri
authors.initials: S
authors.lastname: Pattnaik
authors.forename: Sricharan
authors.initials: S
authors.lastname: Nethala
authors.forename: Anjali
authors.initials: A
authors.lastname: Tripathi
authors.forename: Sekaran
authors.initials: S
authors.lastname: Saravanan
authors.forename: Ambigapathi
authors.initials: A
authors.lastname: Moorthi
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Selvamurugan
mesh_terms: D000327:Adsorption; D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D001673:Biodegradation, Environmental; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D005612:Freeze Drying; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D012822:Silicon Dioxide; D048271:Chitosan; D015040:Zirconium; D009113:Muramidase; C028541:zirconium oxide