title: Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi.
abstract: Infestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. Of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. The importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. For the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion.
authors.affiliation: Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Diogo V
authors.initials: DV
authors.lastname: Lovato
authors.forename: Rogerio
authors.initials: R
authors.lastname: Amino
authors.forename: Yamile
authors.initials: Y
authors.lastname: González
authors.forename: Antonio
authors.initials: A
authors.lastname: Miranda
authors.forename: Sergio
authors.initials: S
authors.lastname: Schenkman
authors.forename: Aparecida S
authors.initials: AS
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D014355:Chagas Disease / Q000276:immunology / Q000517:prevention & control*; D004847:Epithelial Cells / Q000469:parasitology; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007303:Insect Vectors / Q000378:metabolism* / Q000469:parasitology; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D020860:Subtilisin / Q000037:antagonists & inhibitors*; D014224:Triatoma / Q000378:metabolism* / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D019476:Insect Proteins; D012333:RNA, Messenger; C467902:infestin protein, Triatoma infestans; D020860:Subtilisin