title: European 2--a clonal complex of Mycobacterium bovis dominant in the Iberian Peninsula.
abstract: Mycobacterium bovis isolates from the Iberian Peninsula are dominated by strains with spoligotype patterns deleted for spacer 21. Whole-genome sequencing of three Spanish strains with spacer 21 missing in their spoligotype pattern revealed a series of SNPs and subsequent screening of a selection of these SNPs identified one in gene guaA that is specific to these strains. This group of strains from the Iberian Peninsula missing spoligotype spacer 21 represents a new clonal complex of M. bovis, defined by the SNP profile with a distinct spoligotype signature. We have named this clonal complex European 2 (Eu2) and found that it was present at low frequency in both France and Italy and absent from the British Isles.
authors.affiliation: Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, 28040 Madrid, Spain. sabrina.rodriguez@visavet.ucm.es
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rodriguez-Campos
authors.forename: Anita C
authors.initials: AC
authors.lastname: Schürch
authors.forename: James
authors.initials: J
authors.lastname: Dale
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: Lohan
authors.forename: Mónica V
authors.initials: MV
authors.lastname: Cunha
authors.forename: Ana
authors.initials: A
authors.lastname: Botelho
authors.forename: Krystel
authors.initials: K
authors.lastname: De Cruz
authors.forename: M Laura
authors.initials: ML
authors.lastname: Boschiroli
authors.forename: M Beatrice
authors.initials: MB
authors.lastname: Boniotti
authors.forename: Maria
authors.initials: M
authors.lastname: Pacciarini
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Garcia-Pelayo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Romero
authors.forename: Lucía
authors.initials: L
authors.lastname: de Juan
authors.forename: Lucas
authors.initials: L
authors.lastname: Domínguez
authors.forename: Stephen V
authors.initials: SV
authors.lastname: Gordon
authors.forename: Dick
authors.initials: D
authors.lastname: van Soolingen
authors.forename: Brendan
authors.initials: B
authors.lastname: Loftus
authors.forename: Stefan
authors.initials: S
authors.lastname: Berg
authors.forename: R Glyn
authors.initials: RG
authors.lastname: Hewinson
authors.forename: Alicia
authors.initials: A
authors.lastname: Aranaz
authors.forename: Noel H
authors.initials: NH
authors.lastname: Smith
mesh_terms: D000818:Animals; D002417:Cattle; D060965:Clonal Evolution; D005602:France; D016680:Genome, Bacterial; D023281:Genomics; D007558:Italy; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D058974:Phylogeography; D020641:Polymorphism, Single Nucleotide; D011174:Portugal; D013030:Spain