title: Chitosan-based ultrathin films as antifouling, anticoagulant and antibacterial protective coatings.
abstract: Ultrathin antifouling and antibacterial protective nanocoatings were prepared from ionic derivatives of chitosan using layer-by-layer deposition methodology. The surfaces of silicon, and glass protected by these nanocoatings were resistant to non-specific adsorption of proteins disregarding their net charges at physiological conditions (positively charged TGF-β1 growth factor and negatively charged bovine serum albumin) as well as human plasma components. The coatings also preserved surfaces from the formation of bacterial (Staphylococcus aureus) biofilm as shown using microscopic studies (SEM, AFM) and the MTT viability test. Moreover, the chitosan-based films adsorbed onto glass surface demonstrated the anticoagulant activity towards the human blood. The antifouling and antibacterial actions of the coatings were correlated with their physicochemical properties. The studied biologically relevant properties were also found to be dependent on the thickness of those nanocoatings. These materials are promising for biomedical applications, e.g., as protective coatings for medical devices, anticoagulant coatings and protective layers in membranes.
authors.affiliation: a Faculty of Chemistry, Jagiellonian University , Ingardena 3 , 30-060 , Krakow , Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bulwan
authors.forename: Kinga
authors.initials: K
authors.lastname: Wójcik
authors.forename: Szczepan
authors.initials: S
authors.lastname: Zapotoczny
authors.forename: Maria
authors.initials: M
authors.lastname: Nowakowska
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D000925:Anticoagulants / Q000737:chemistry; D057219:Biofouling / Q000517:prevention & control*; D001769:Blood / Q000187:drug effects / Q000382:microbiology; D002119:Calcium Carbonate; D002417:Cattle; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005898:Glass; D006801:Humans; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012825:Silicon; D013211:Staphylococcus aureus / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D053773:Transforming Growth Factor beta1; D012710:Serum Albumin, Bovine; D048271:Chitosan; D002119:Calcium Carbonate; D012825:Silicon