title: Platelet-rich plasma induces increased expression of G1 cell cycle regulators,             type I collagen, and matrix metalloproteinase-1 in human skin fibroblasts.
abstract: Platelet-rich plasma (PRP) is derived from fresh whole blood, which contains a high concentration of platelets. Recently, PRP has been used for skin wound healing and rejuvenation. However, the molecular mechanisms underlying PRP-inducing wound healing processes are still largely unknown. The aim of this study is to evaluate the effect of PRP on the expression of G1 cell cycle regulatory proteins, type I collagen, matrix metalloproteinase-1 (MMP-1), and MMP-2 in human skin fibroblasts (HSF). We performed a cell proliferation and a migration assay, immunoblotting, and a chloramphenicol acetyltransferase (CAT) assay in PRP-treated human skin fibroblasts. PRP treatment induced increased rates of cell proliferation and cell migration. Expression of cyclin A protein was increased by a low concentration (0.5%) of PRP-treated HSF. In addition, expression of Rb, cyclin E, and cyclin-dependent kinase 4 proteins was increased by a high concentration (5%) of PRP-treated HSF. High concentration of PRP induced an up-regulation of type I collagen, MMP-1, and MMP-2 expression in HSF. Taken together, PRP treatment induced an increase in expression of G1 cell cycle regulators, type I collagen and MMP-1, thereby accelerating the wound healing process.
authors.affiliation: Department of Dermatology, Keimyung University School of Medicine, Daegu, Republic of Korea.
authors.forename: Jae-We
authors.initials: JW
authors.lastname: Cho
authors.forename: Sung-Ae
authors.initials: SA
authors.lastname: Kim
authors.forename: Kyu-Suk
authors.initials: KS
authors.lastname: Lee
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D016160:Retinoblastoma Protein / Q000378:metabolism; D014945:Wound Healing
chemical_list: D018797:Cell Cycle Proteins; D024042:Collagen Type I; D016160:Retinoblastoma Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1