title: Cellular migration to electrospun poly(lactic acid) fibermats.
abstract: Nonwoven fabrics prepared via an electrospinning method, so-called electrospun fibermats, are expected to be promising scaffold materials for bone tissue engineering. In the present work, poly(L-lactic acid) (PLLA) fibermats, consisting of fibers with diameters ranging from 1 to 10 μm, were prepared by electrospinning. Mouse osteoblast-like cells (MC3T3-E1) were seeded on the fibermats with various fiber diameters (10, 5 and 2 μm; they are denoted by samples A, B and C, respectively) and cultured in two different directions in order to compare the migration behaviours into the scaffold of the normal condition and the anti-gravity condition. The cells in/on the fibermats were observed by laser confocal microscopy to estimate the cellular migration ability into them. When the MC3T3-E1 cells were cultured in the normal direction, the thickness of their layer increased to approx. 90 μm in the sample A, consisting of 10-μm fibers after 13 days of culture, while that in the sample C, consisting of 2-μm fibers, did not increase. When the MC3T3-E1 cells were cultured in the anti-gravity condition, the thickness of the cell layer in the sample A increased to approx. 60 μm. These results mean that the MC3T3-E1 cells migrated into the inside of sample A in either the normal direction or the anti-gravity one. The cellular proliferation showed no significant difference among the fibermats with three different fiber diameters; MC3T3-E1 cells on the fibermat with 2 μm fiber diameter grew two-dimensionally, while they grew three-dimensionally in the fibermat with 10 μm fiber diameter.
authors.affiliation: a Department of Frontier Materials , Graduate School of Engineering, Nagoya Institute of Technology , Gokiso-cho , Showa-ku , Nagoya , 466-8555 , Japan.
authors.forename: Kie
authors.initials: K
authors.lastname: Fujikura
authors.forename: Akiko
authors.initials: A
authors.lastname: Obata
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kasuga
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry; D002465:Cell Movement / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D019344:Lactic Acid / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D013997:Time Factors; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)