title: Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments.
abstract: Hydroxyapatite (HA) is a material of choice for bone grafts owing to its chemical and structural similarities to the mineral phase of hard tissues. The combination of osteogenic proteins with HA materials that carry and deliver the proteins to the bone-defective areas will accelerate bone regeneration. The study investigated the treatment of HA particles with different amino acids such as serine (Ser), asparagine (Asn), aspartic acid (Asp) and arginine (Arg) to enhance the adsorption ability of HA carrier for delivering therapeutic proteins to the body. The crystallinity of HA reduced when amino acids were added during HA preparation. Depending on the types of amino acid, the specific surface area of the amino acid-functionalized HA particles varied from 105 to 149 m(2) g(-1). Bovine serum albumin (BSA) and lysozyme were used as model proteins for adsorption study. The protein adsorption onto the surface of amino acid-functionalized HA depended on the polarities of HA particles, whereby, compared with lysozyme, BSA demonstrated higher affinity towards positively charged Arg-HA. Alternatively, the binding affinity of lysozyme onto the negatively charged Asp-HA was higher when compared with BSA. The BSA and lysozyme adsorptions onto the amino acid-functionalized HA fitted better into the Freundlich than Langmuir model. The amino acid-functionalized HA particles that had higher protein adsorption demonstrated a lower protein-release rate.
authors.affiliation: Advanced Drug Delivery Group, Faculty of Pharmacy, University of Sydney, Sydney, New South Wales 2006, Australia.
authors.forename: Wing-Hin
authors.initials: WH
authors.lastname: Lee
authors.forename: Ching-Yee
authors.initials: CY
authors.lastname: Loo
authors.forename: Kim Linh
authors.initials: KL
authors.lastname: Van
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Zavgorodniy
authors.forename: Ramin
authors.initials: R
authors.lastname: Rohanizadeh
mesh_terms: D000327:Adsorption; D000596:Amino Acids / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000596:Amino Acids; D011506:Proteins; D012710:Serum Albumin, Bovine; D017886:Durapatite