title: Osteogenic gene expression of canine bone marrow stromal cell and bacterial adhesion on titanium with different nanotubes.
abstract: Bacterial infection and osseointegration of implant-biomaterials all play important roles in the success of an orthopedic prosthesis or a dental-implant. In this work, we evaluated the osteogenic gene expression of canine bone marrow stromal cells (CBMSCs) and the adhesion of Staphylococcus aureus (ATCC 12598) on different diameter TiO(2) nanotube layers. The CBMSCs cultured on 30 and 70 nm nanotubes displayed polygon shape, but obviously elongated when the diameter of nanotubes turned to 120 nm. A significant increase in CBMSCs proliferation by as much as about ∼300%, and osteogenic gene (RUNX-2, OPN, COL-1, and OCN) expression were observed on the 120 nm diameter nanotubes when compared to the smooth Ti. However, the adhesion of bacteria also increased with an increased tube diameter and reached highest value on 120 nm nanotubes after 4 h of incubation. ∼300-400% increase in bacterial attached to 120 nm nanotubes in contract to the smooth Ti. These data suggested reducing bacteria colonization should be considered when larger diameter nanotubes with better osteogenic property would be used as orthopedic implants or dental implants.
authors.affiliation: Department of Prosthodontics, School of Stomatology and Affiliated Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Research Institute of Stomatology, Shanghai 200011, China.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Yu
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Jiang
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhao
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D004285:Dogs; D005786:Gene Expression Regulation; D008297:Male; D043942:Nanotubes / Q000737:chemistry*; D010012:Osteogenesis; D019736:Prostheses and Implants; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology*; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D014025:Titanium