title: Plant steroid hormones produced under Ni stress are involved in the regulation of metal uptake and oxidative stress in Brassica juncea L.
abstract: Brassinosteroids (BRs) are involved in the amelioration of various biotic and abiotic stresses. With an aim to explore the role of BRs under heavy metal stress, plants of Brassica juncea L. were grown in pots. The plants were subjected to various concentrations of Nickel metal (0.0, 0.2, 0.4 and 0.6 mM) and harvested on 60th day in order to observe the expression of these hormones. The isolated BRs from the leaves of Brassica plants characterized by GC-MS include 24-Epibrassinolide (24-EBL), Castasterone, Dolicholide and Typhasterole. The effect of isolated 24-EBL was studied on Ni metal uptake and antioxidative defense system in 60 d old plants of Brassica. It was observed that 24-EBL significantly increased the activities of stress ameliorating enzymes and lowered the metal uptake in plants. This is the first report in B. juncea L. plants showing the expression of BRs under metal treatments and effect of the isolated 24-EBL on metal uptake and in oxidative stress management.
authors.affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143 005, Punjab, India.
authors.forename: Mukesh Kumar
authors.initials: MK
authors.lastname: Kanwar
authors.forename: Renu
authors.initials: R
authors.lastname: Bhardwaj
authors.forename: Priya
authors.initials: P
authors.lastname: Arora
authors.forename: Sikandar Pal
authors.initials: SP
authors.lastname: Chowdhary
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sharma
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
mesh_terms: D060406:Brassinosteroids / Q000378:metabolism* / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D008401:Gas Chromatography-Mass Spectrometry; D009149:Mustard Plant / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000378:metabolism* / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D060406:Brassinosteroids; D010937:Plant Growth Regulators; D013260:Steroids, Heterocyclic; D009532:Nickel; C023623:brassinolide