title: Target cell-mediated editing of HIV-1 cDNA by APOBEC3 proteins in human macrophages.
abstract: Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins are encapsidated by assembling HIV-1 virions and edit viral cDNA in the next round of infection. Using alpha interferon (IFN-α)-treated monocyte-derived macrophages, we show that infrequent editing of HIV-1 reverse transcripts can also be mediated by APOBEC3 proteins supplied by the targets of infection. Based on the local sequence contexts of these mutations and the established characteristics of APOBEC3 protein expression in myeloid cells, we speculate that APOBEC3A may be responsible for a substantial proportion of this activity.
authors.affiliation: Department of Infectious Diseases, King's College London School of Medicine, 2nd Floor, Borough Wing, Guy's Hospital, London Bridge, London SE1 9RT, United Kingdom.
authors.forename: Fransje A
authors.initials: FA
authors.lastname: Koning
authors.forename: Caroline
authors.initials: C
authors.lastname: Goujon
authors.forename: Hélène
authors.initials: H
authors.lastname: Bauby
authors.forename: Michael H
authors.initials: MH
authors.lastname: Malim
mesh_terms: D000071478:APOBEC Deaminases; D003564:Cytidine Deaminase; D043525:Cytosine Deaminase / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D008264:Macrophages / Q000201:enzymology* / Q000821:virology*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D043525:Cytosine Deaminase; D000071478:APOBEC Deaminases; C502889:APOBEC3 proteins, human; D003564:Cytidine Deaminase