title: Effect of vardenafil on nitric oxide synthase expression in the paraventricular nucleus of rats without sexual stimulation.
abstract: Vardenafil hydrochloride (HCl) is a potent and selective phosphodiesterase type-5 (PDE-5) inhibitor that enhances nitric oxide (NO)-mediated relaxation of human corpus cavernosum and NO-induced rabbit penile erection, and enhances erectile function in patients. In the present study, the effect of vardenafil on nitric oxide synthase (NOS) and neuronal NOS expressions in the paraventricular nucleus (PVN) of rats without sexual stimulation was investigated using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and neuronal NOS (nNOS) immunohistochemistry and western blot analysis. The present results showed that NOS and nNOS expression in the PVN was increased by vardenafil treatment as the dose- and duration-dependently without sexual stimulation. The phosphodiesterase type-5 inhibitor, vardenafil, augmented NOS expression in the brain without sexual stimulation. The present study suggests that sexual behaviour can be directly modulated by neurotransmitters such as nitric oxide.
authors.affiliation: Department of Urology, Gachon University Gil Hospital, Gachon University of Medicine and Science, Incheon, Korea.
authors.forename: M-S
authors.initials: MS
authors.lastname: Shin
authors.forename: I-G
authors.initials: IG
authors.lastname: Ko
authors.forename: S-E
authors.initials: SE
authors.lastname: Kim
authors.forename: B-K
authors.initials: BK
authors.lastname: Kim
authors.forename: C-J
authors.initials: CJ
authors.lastname: Kim
authors.forename: D-H
authors.initials: DH
authors.lastname: Kim
authors.forename: S-J
authors.initials: SJ
authors.lastname: Yoon
authors.forename: K-H
authors.initials: KH
authors.lastname: Kim
mesh_terms: D000818:Animals; D015153:Blotting, Western; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5 / Q000187:drug effects*; D007093:Imidazoles / Q000494:pharmacology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects* / Q000201:enzymology; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013450:Sulfones / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology; D000069058:Vardenafil Dihydrochloride
chemical_list: D007093:Imidazoles; D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D013450:Sulfones; D014227:Triazines; D000069058:Vardenafil Dihydrochloride; D052248:Nitric Oxide Synthase Type I; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5