title: Is low-intensity pulsed ultrasound effective for revitalizing a severely necrotic small bone? An experimental rabbit model.
abstract: Previously, we successfully applied a new method composed of drilling, bone marrow transplantation (BMT), external fixation and low-intensity pulsed ultrasound (LIPUS) for the clinical treatment of Kienböck's disease. The purpose of this study was to investigate whether bone regeneration can be induced by LIPUS and/or multiple drilling and/or BMT within a severely necrotic small-bone rabbit model. Eighteen rabbits were divided into three groups (BMT, drilling and control) and LIPUS stimulation was introduced daily for 8 weeks post-transplantation. Next, 12 additional rabbits were produced for the BMT group and LIPUS stimulation was introduced daily for 4 and 12 weeks (n = 6 for each). Histopathologically, new bone formations were rarely observed in the drilling and control groups. In the BMT group, the mineralizing surface areas of LIPUS(+) showed a significant increase compared with LIPUS(-) for 8 weeks. LIPUS treatment alone did not accelerate the revitalization of necrotic bones. However, LIPUS combined with BMT tended to promote new bone formation.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. oga-take@pg7.so-net.ne.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Tomoo
authors.initials: T
authors.lastname: Ishii
authors.forename: Hajime
authors.initials: H
authors.lastname: Mishima
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Nishino
authors.forename: Arata
authors.initials: A
authors.lastname: Watanabe
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Ochiai
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology* / Q000528:radiation effects*; D004195:Disease Models, Animal; D005260:Female; D019220:High-Energy Shock Waves; D006801:Humans; D010020:Osteonecrosis / Q000175:diagnosis / Q000628:therapy*; D011817:Rabbits; D011829:Radiation Dosage; D016896:Treatment Outcome; D014464:Ultrasonic Therapy / Q000379:methods*; D018617:Ultrasonography, Doppler, Pulsed