title: Experimental infection of cattle, sheep and pigs with 'Hobi'-like pestivirus.
abstract: To date, limited information is available on the ability of 'Hobi'-like pestiviruses (putative bovine viral diarrhoea 3) to infect and cause disease in animal species traditionally affected by pestiviruses. In order to obtain new insights into host range and pathogenic potential of this atypical pestivirus, BVDV-seronegative calves (n=5), lambs (n=5) and piglets (n=5) were experimentally infected with the European 'Hobi'-like strain Italy-1/10-1, whereas two animals per species served as uninfected controls. Appearance of clinical signs, leukopenia, viremia, viral shedding and seroconversion were monitored for 28 days post-infection. Calves and lambs were successfully infected, displaying respiratory signs (nasal discharge), moderate hyperthermia and leukopenia, viremia and viral shedding through the nasal and faecal routes. Antibody responses were observed in both animal species by ELISA and virus neutralisation assays. In contrast, inoculated piglets did not display any clinical signs nor leukopenia and viral RNA was not detected in any biological samples. Nevertheless, the presence of detectable antibodies by virus neutralisation accounted for a successful, albeit limited infection of these animals.
authors.affiliation: Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy. n.decaro@veterinaria.uniba.it
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Viviana
authors.initials: V
authors.lastname: Mari
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Lucente
authors.forename: Rossana
authors.initials: R
authors.lastname: Sciarretta
authors.forename: Ana
authors.initials: A
authors.lastname: Moreno
authors.forename: Carlo
authors.initials: C
authors.lastname: Armenise
authors.forename: Michele
authors.initials: M
authors.lastname: Losurdo
authors.forename: Michele
authors.initials: M
authors.lastname: Camero
authors.forename: Eleonora
authors.initials: E
authors.lastname: Lorusso
authors.forename: Paolo
authors.initials: P
authors.lastname: Cordioli
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D007558:Italy; D010576:Pestivirus / Q000276:immunology / Q000472:pathogenicity*; D018182:Pestivirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D014766:Viremia / Q000662:veterinary; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral