title: Immune-related genes expression profile in orange-spotted grouper during exposure to Cryptocaryon irritans.
abstract: Cryptocaryon irritans is one of the most important ectoparasites of marine fish. To identify the potential role of immune-related genes in antiparasitic immune responses in fish, we monitored the expression change of IL-8, COX-2, C-type lectin and transferrin in local and systemic immune organs of orange-spotted grouper post-C. irritans infection. IL-8 expression was up-regulated during the course of infection in the skin, while COX-2 and transferrin expression was up-regulated in the gill. COX-2 expression was significantly down-regulated in the spleen (0·7-5% of its control) and head kidney (0·5-4% of its control) post-primary infection. Transferrin expression was also down-regulated in the spleen and head kidney from 6 h to 5 days post-primary infection. However, C-type lectin expression was up-regulated in all tested organs post-infection, with the exception of day 7 in the spleen post-primary infection where the expression level was slightly down-regulated (44% of its control). These results suggest that these four immune-related genes play an important role in grouper anti-C. irritans infection and that local immune organs as the active organs contribute more than systemic immune organs to this course.
authors.affiliation: Key Laboratory for Aquatic Products Safety of Ministry of Education/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Li
authors.forename: X M
authors.initials: XM
authors.lastname: Dan
authors.forename: T W
authors.initials: TW
authors.lastname: Zhang
authors.forename: X C
authors.initials: XC
authors.lastname: Luo
authors.forename: A X
authors.initials: AX
authors.lastname: Li
mesh_terms: D000818:Animals; D001492:Bass / Q000276:immunology* / Q000469:parasitology*; D016798:Ciliophora / Q000276:immunology* / Q000472:pathogenicity*; D016770:Ciliophora Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology*; D020869:Gene Expression Profiling; D016209:Interleukin-8 / Q000096:biosynthesis; D037181:Lectins, C-Type / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D014168:Transferrin / Q000096:biosynthesis
chemical_list: D016209:Interleukin-8; D037181:Lectins, C-Type; D014168:Transferrin; D051546:Cyclooxygenase 2