title: Carbon nanotube-based ultrasensitive multiplexing electrochemical immunosensor for cancer biomarkers.
abstract: A multiplexing electrochemical immunosensor was developed for ultrasensitive detection of cancer related protein biomarkers. We employed disposable screen-printed carbon electrode (SPCE) array as the detection platform. A universal multi-labeled nanoprobe was developed by loading HRP and goat-anti-rabbit IgG (secondary antibody, Ab(2)) onto multiwalled carbon nanotube (MWNT). This universal nanoprobe was available for virtually any sandwich-based antigen detection and showed superiority in several areas. By using the SPCE array and the universal nanoprobe, we could detect as low as 5 pg mL(-1) of prostate specific antigen (PSA) and 8 pg mL(-1) of Interleukin 8 (IL-8) with the electrochemical immunosensor. We also demonstrated simultaneous detection of two protein biomarkers with this platform. With these attracted features, our immunoassay system shows promising applications for in-field and point-of-care test in clinical diagnostics.
authors.affiliation: School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wan
authors.forename: Wangping
authors.initials: W
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhu
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.forename: Haiyan
authors.initials: H
authors.lastname: Hu
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Peng
authors.forename: Shiping
authors.initials: S
authors.lastname: Song
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry; D009363:Neoplasm Proteins / Q000032:analysis*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D045424:Complex Mixtures; D037742:Nanotubes, Carbon; D009363:Neoplasm Proteins