title: Zn-binding AZUL domain of human ubiquitin protein ligase Ube3A.
abstract: Ube3A (also referred to as E6AP for E6 Associated Protein) is a E3 ubiquitin-protein ligase implicated in the development of Angelman syndrome by controlling degradation of synaptic protein Arc and oncogenic papilloma virus infection by controlling degradation of p53. This article describe the solution NMR structure of the conserved N-terminal domain of human Ube3A (residues 24-87) that contains two residues (Cys44 and Arg62) found  to be mutated in patients with Angelman syndrome. The structure of this domain  adopts a novel Zn-binding  fold we called AZUL (Amino-terminal Zn-finger of Ube3a Ligase). The AZUL  domain has a helix-loop-helix architecture with a Zn ion coordinated by four Cys residues arranged in Cys-X(4)-Cys-X(4)-Cys-X(28)-Cys motif. Three of the Zn-bound residues are located in a 23-residue long and well structured loop that connects two α-helicies.
authors.affiliation: Ontario Cancer Institute, Campbell Family Cancer Research Institute and Department of Medical Biophysics, University of Toronto, and Northeast Structural Genomics Consortium, 101 College Street, Toronto, ON M5G 1L7, Canada.
authors.forename: Alexander
authors.initials: A
authors.lastname: Lemak
authors.forename: Adelinda
authors.initials: A
authors.lastname: Yee
authors.forename: Irina
authors.initials: I
authors.lastname: Bezsonova
authors.forename: Sirano
authors.initials: S
authors.lastname: Dhe-Paganon
authors.forename: Cheryl H
authors.initials: CH
authors.lastname: Arrowsmith
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000378:metabolism; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism
chemical_list: C471639:UBE3A protein, human; D044767:Ubiquitin-Protein Ligases; D015032:Zinc