title: Correlation of synovial fluid HMGB-1 levels with radiographic severity of knee osteoarthritis.
abstract: OBJECTIVE
  This study measured high-mobility group box 1 (HMGB-1) levels in serum and synovial fluid (SF) in patients with primary knee osteoarthritis (OA) and correlated these levels with radiographic disease severity.
  
  
  METHODS
  Seventy-eight OA patients and 30 controls were enrolled in this study. All OA patients were scored according to the Kellgren-Lawrence (KL) grading system. HMGB-1 levels were measured by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  SF HMGB-1 levels were significantly higher in knee OA patients, compared with controls (P < 0.01). Moreover, SF HMGB-1 levels were positively associated with KL scores (P < 0.01). Multinomial logistic regression demonstrated that the SF HMGB-1 level was an independent factor for radiographic severity of OA (P=0.002); however, serum HMGB-1 levels did not differ significantly between OA patients and controls and did not correlate with KL scores (P > 0.05).
  
  
  CONCLUSIONS
  These results demonstrate that HMGB-1 levels in SF of knee OA patients are independently associated with radiographic disease severity.
authors.affiliation: Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiaotong University School of Medicine, China. shliuzude@126.com
authors.forename: Zhan-Chun
authors.initials: ZC
authors.lastname: Li
authors.forename: Guang-Qi
authors.initials: GQ
authors.lastname: Cheng
authors.forename: Kong-Zu
authors.initials: KZ
authors.lastname: Hu
authors.forename: Mao-Qiang
authors.initials: MQ
authors.lastname: Li
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zang
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Dong
authors.forename: Wei-Li
authors.initials: WL
authors.lastname: Wang
authors.forename: Zu-De
authors.initials: ZD
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D024243:HMGB1 Protein / Q000097:blood* / Q000378:metabolism*; D006801:Humans; D007719:Knee Joint / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000097:blood* / Q000000981:diagnostic imaging* / Q000378:metabolism*; D011859:Radiography; D013582:Synovial Fluid / Q000737:chemistry*
chemical_list: D024243:HMGB1 Protein