title: UHPLC-PDA-ESI/HRMS/MS(n) analysis of anthocyanins, flavonol glycosides, and hydroxycinnamic acid derivatives in red mustard greens (Brassica juncea Coss variety).
abstract: An UHPLC-PDA-ESI/HRMS/MS(n) profiling method was used for a comprehensive study of the phenolic components of red mustard greens ( Brassica juncea  Coss variety) and identified 67 anthocyanins, 102 flavonol glycosides, and 40 hydroxycinnamic acid derivatives. The glycosylation patterns of the flavonoids were assigned on the basis of direct comparison of the parent flavonoid glycosides with reference compounds. The putative identifications were obtained from tandem mass data analysis and confirmed by the retention time, elution order, and UV-vis and high-resolution mass spectra. Further identifications were made by comparing the UHPLC-PDA-ESI/HRMS/MS(n) data with those of reference compounds in the polyphenol database and in the literature. Twenty-seven acylated cyanidin 3-sophoroside-5-diglucosides, 24 acylated cyanidin 3-sophoroside-5-glucosides, 3 acylated cyanidin triglucoside-5-glucosides, 37 flavonol glycosides, and 10 hydroxycinnamic acid derivatives were detected for the first time in brassica vegetables. At least 50 of them are reported for the first time in any plant materials.
authors.affiliation: Food Composition and Methods Development Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, US Department of Agriculture, Building-161, BARC-East, 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States. Longze.lin@are.usda.gov
authors.forename: Long-Ze
authors.initials: LZ
authors.lastname: Lin
authors.forename: Jianghao
authors.initials: J
authors.lastname: Sun
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.forename: James
authors.initials: J
authors.lastname: Harnly
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis*; D044948:Flavonols / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D009149:Mustard Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000872:Anthocyanins; D003373:Coumaric Acids; D044948:Flavonols; D006027:Glycosides; D010936:Plant Extracts