title: Osteoanagenesis after transplantation of bone marrow-derived mesenchymal stem cells using polyvinylidene chloride film as a scaffold.
abstract: The aim of this study was to develop a new cell transplantation technique for osteoanagenesis at bone defect sites. Polyvinylidene chloride (PVDC) film was evaluated because of its good biocompatibility and flexibility. We used this film as both a cell scaffold and a barrier membrane. Initially, the cell compatibility of the PVDC film for fibroblast-like cells and osteoblast-like cells was confirmed. Subsequently, bone marrow cells were obtained from rats and cultured on PVDC films in two kinds of medium. The PVDC films with bone marrow-derived mesenchymal stem cells (MSCs) were then applied to critical-sized bone defects in the calvarial bone of rats. After the transplantation, the surgical sites were dissected out and evaluated by soft X-ray radiography, micro-CT analysis and histological examinations. The bone marrow-derived MSC-transplanted rats showed greater bone regeneration than the control rats. Therefore, PVDC film is considered to be useful as a scaffold for bone regeneration.
authors.affiliation: Department of Dental Materials Science, School of Dentistry, Aichi Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. hamajima@dpc.aichi-gakuin.ac.jp
authors.forename: Soichiro
authors.initials: S
authors.lastname: Hamajima
authors.forename: Tatsuhide
authors.initials: T
authors.lastname: Hayashi
authors.forename: Yamato
authors.initials: Y
authors.lastname: Sato
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sasaki
authors.forename: Tatsushi
authors.initials: T
authors.lastname: Kawai
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials; D001843:Bone Cements / Q000737:chemistry; D001847:Bone Diseases / Q000601:surgery; D001854:Bone Marrow Cells / Q000166:cytology*; D001861:Bone Regeneration / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D005347:Fibroblasts / Q000502:physiology; D008297:Male; D008567:Membranes, Artificial; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D011138:Polytetrafluoroethylene / Q000737:chemistry; D011143:Polyvinyl Chloride / Q000031:analogs & derivatives*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000601:surgery; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D001672:Biocompatible Materials; D001843:Bone Cements; C498353:G-Bond; D008567:Membranes, Artificial; D008689:Methacrylates; D011138:Polytetrafluoroethylene; C029547:polyvinylidene chloride; D011143:Polyvinyl Chloride; D000469:Alkaline Phosphatase