title: Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland.
abstract: The production, preliminary characterisation and applications of monoclonal antibodies (mAbs) against two novel swine bocaviruses isolated in cell culture from swine in Northern Ireland are described. Of the 17 stable final clones produced, four were characterised. All were of the IgG2a isotype and showed no cross-reactivity with either bocavirus strain. Partial neutralisation was observed with PBoV4 mAbs and homologous virus. The two mAbs selected for use in antigen-detecting ELISAs were successful in highlighting those fractions containing infectious virus within sucrose gradients. This is the first report of the production of specific reagents that will prove useful in the study of the biology of these viruses and swine bocavirus-associated diseases.
authors.affiliation: Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stormont, Belfast, Northern Ireland, UK. Irene.mcnair@afbini.gov.uk
authors.forename: I
authors.initials: I
authors.lastname: McNair
authors.forename: F
authors.initials: F
authors.lastname: McNeilly
authors.forename: C
authors.initials: C
authors.lastname: Duffy
authors.forename: J
authors.initials: J
authors.lastname: McKillen
authors.forename: M
authors.initials: M
authors.lastname: McMenamy
authors.forename: M
authors.initials: M
authors.lastname: Welsh
authors.forename: G
authors.initials: G
authors.lastname: Allan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000032:analysis; D052660:Bocavirus / Q000276:immunology* / Q000302:isolation & purification* / Q000472:pathogenicity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D009660:Northern Ireland; D010322:Parvoviridae Infections / Q000662:veterinary / Q000821:virology; D034421:Sus scrofa / Q000821:virology*; D013552:Swine; D013553:Swine Diseases / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G