title: Isolation, expansion, and differentiation of goat adipose-derived stem cells.
abstract: A goat adipose-derived stem cell (ADSC) line was established and compared to a rat line. Goat ADSC cells had normal diploidy after subculture. Proliferation of goat ADSCs was faster than rat cells in the same conditions. Both rat and goat ADSCs stained positively for vimentin, CD49d, CD44 and CD13, but stained negatively for CD34 and CD106. Bone nodules were apparent, and alizarin staining was positive after osteogenic induction. Cells expressing osteocalcin were positive by alkaline phosphatase (ALP) staining. After osteogenic induction, ossification nodules of goat ADSCs were larger than in rats, with dense ALP staining. Adipogenic induction resulting in lipid droplets and peroxisome proliferator-activated receptor (PPARγ2) expression were observed. Cartilage lacunae were formed and COL2A1 was expressed. More cartilage lacunae with better morphology were seen following differentiation of goat ADSC's using the hang-drop method. For goat ADSCs, results with both adherent-induced and hanging-drop induced cultures were better than for three-dimensional cultures.
authors.affiliation: Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University, Inner Mongolia, Hohhot 010021, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ren
authors.forename: Haiqing
authors.initials: H
authors.lastname: Wu
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Jianxun
authors.initials: J
authors.lastname: Wen
authors.forename: Muzi
authors.initials: M
authors.lastname: Jin
authors.forename: Ming
authors.initials: M
authors.lastname: Cang
authors.forename: Xudong
authors.initials: X
authors.lastname: Guo
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Wang
authors.forename: Dongjun
authors.initials: D
authors.lastname: Liu
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002875:Chromosomes / Q000502:physiology / Q000648:ultrastructure; D004396:Coloring Agents; D006041:Goats / Q000502:physiology; D008297:Male; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure
chemical_list: D004396:Coloring Agents