title: [Prognostic value on recovery rates for the application of sperm preparation techniques and their evaluation in sperm function].
abstract: OBJECTIVE
  To evaluate sperm recovery and total sperm motility in three different sperm preparation techniques (density gradient, simple washing and swim-up).
  
  
  METHODS
  A total of 290 subjects were randomly evaluated from November 2001 to March 2003. The density gradient method required Isolate (upper and lower layers). Centrifugation was performed at 400 g for 10 minutes and evaluation was done using the Makler counting chamber. The simple washing method included the use of HTF-M complemented with 7.5% of SSS, with centrifugation at 250 g, obtaining at the end 0.5 mL of the sperm sample. The swim-up method required HTF-M complemented with 7.5% of SSS, with an incubation period of 60 minutes at 37 degrees C.
  
  
  RESULTS
  The demographic characteristics evaluated through their standard error, 95% ICC, and 50th percentile were similar. The application of multiple comparison tests and analysis of variance showed significant differences between the sperm preparations before and after capacitation. It was observed a superior recovery rate with the density gradient and swim-up methods; nevertheless, the samples used for the simple washing method showed a diminished sperm recovery from the original sample.
  
  
  CONCLUSIONS
  Sperm preparation techniques have become very useful in male infertility treatments allowing higher sperm recovery and motility rates. The seminal parameters evaluated from the original sperm sample will determine the best sperm preparation technique in those patients who require it.
authors.affiliation: Unidad de Reproducción Asistida, Departamento de Ginecología y Obstetricia, Clínica Lomas Altas, Ciudad de México. gbarroso@clinicalomas.com.mx
authors.forename: Gerardo
authors.initials: G
authors.lastname: Barroso
authors.forename: Miguel
authors.initials: M
authors.lastname: Chaya
authors.forename: Rubén
authors.initials: R
authors.lastname: Bolaños
authors.forename: Yadira
authors.initials: Y
authors.lastname: Rosado
authors.forename: Fernando
authors.initials: F
authors.lastname: García León
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ibarrola
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D012494:Sampling Studies; D013048:Specimen Handling / Q000379:methods; D013075:Sperm Capacitation; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*; D020858:Tissue and Organ Harvesting / Q000379:methods*