title: Postmortem changes in pork muscle protein phosphorylation in relation to the RN genotype.
abstract: Postmortem changes in pork muscle protein phosphorylation in relation to the RN(-) genotype were investigated using one-dimensional gel electrophoresis and a phosphor specific staining. The phosphorylation levels of several protein bands were found to be affected by the RN(-) genotype and to change during postmortem development. Glycogen phosphorylase, phosphofructokinase, and pyruvate kinase were found in protein bands affected by the RN(-) genotype, and the phosphorylation profile indicates that part of the increased rate and extended pH decline of the RN(-) genotype could be a consequence of phosphorylation of these key enzymes during the postmortem metabolism. The results illustrate that the protein phosphorylation level of the muscle proteins could be interpreted as a global metabolic fingerprint containing information about the activity status of the enzymes in the postmortem metabolism.
authors.affiliation: Department of Food Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. rla@life.ku.dk
authors.forename: René
authors.initials: R
authors.lastname: Lametsch
authors.forename: Martin R
authors.initials: MR
authors.lastname: Larsen
authors.forename: Birgitta
authors.initials: B
authors.lastname: Essén-Gustavsson
authors.forename: Marianne
authors.initials: M
authors.lastname: Jensen-Waern
authors.forename: Kerstin
authors.initials: K
authors.lastname: Lundström
authors.forename: Gunilla
authors.initials: G
authors.lastname: Lindahl
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D005838:Genotype; D024981:Glycogen Phosphorylase / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology* / Q000378:metabolism; D009154:Mutation; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010766:Phosphorylation; D011180:Postmortem Changes; D011770:Pyruvate Kinase / Q000378:metabolism; D013552:Swine / Q000235:genetics / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D024981:Glycogen Phosphorylase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D055372:AMP-Activated Protein Kinases