title: Protonating and determining myosmine intactly by association with citrate anion.
abstract: Myosmine can not be separated from nornicotine, nicotine and anabasine intactly by capillary zone electrophoresis (CZE) with a phosphate buffer. Using citrate solution at pH 6.5 as a CZE buffer, myosmine is protonated intactly by H(+), charged positively and then separated from other tobacco alkaloids on the baseline. Its sensitivity is ten times higher than gas chromatography (GC) with a nitrogen-phosphorus detector (NPD). The mechanism for protonating myosmine intactly is discussed and the utility of the new method is testified, too.
authors.affiliation: College of Chemistry and Chemical Engineering, Hunan Normal University, P. R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xu
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D002021:Buffers; D002951:Citrates / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D055772:Green Chemistry Technology; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000737:chemistry; D011522:Protons; D015203:Reproducibility of Results; D012996:Solutions; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry
chemical_list: D000470:Alkaloids; D002021:Buffers; D002951:Citrates; D010710:Phosphates; D011522:Protons; D012996:Solutions; C002398:myosmine