title: [Effects of multiwall carbon nano-onions on platelet aggregation and hemostatic function].
abstract: OBJECTIVE
  To observe the effects of multiwall carbon nano-onions (MWCNOs) on platelet aggregation and hemostatic function.
  
  
  METHODS
  The platelet aggregation was determined with Born's method at different concentration of MWCNOs (0, 0.2, 2.0, 20.0 microg/ml) in vitro. Twenty male SD rats were randomly divided into 4 groups which were exposed to 0, 2, 4 and 8 mg/kg MWCNOs, respectively. Then platelet count, platelet aggregation, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), bleeding time (BT) and platelet count (PC) were measured at 12 h after receiving tail intravenous injection of MWCNOs. The effects of MWCNOs (4 mg/kg) on platelet aggregation and platelet count at different time points were observed.
  
  
  RESULTS
  In vitro, MWCNOs exhibited the potent inhibitory effects on rat platelet aggregation caused by ADP in a concentration-dependent manner. The platelet aggregation in the highest dosage of 20.0 microg/ml group was 50.0% +/- 6.9% which was significantly lower than that (73.2% +/- 4.3%) in control group (P<0.01). In vivo, the highest inhibitory was up to 20.4%, but there was no significant difference, as compared with control group. MWCNOs did not affect the APTT, PT, TT, BT and PC.
  
  
  CONCLUSIONS
  Under this experimental condition, MWCNOs might inhibit platelet aggregation but not affect hemostatic function.
authors.affiliation: Department of Toxicology, Zhejiang University School of Public Health, Hangzhou 310058, China.
authors.forename: Gui-li
authors.initials: GL
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yu-ying
authors.initials: YY
authors.lastname: Xu
authors.forename: Qiao-hui
authors.initials: QH
authors.lastname: Wei
authors.forename: Xiang-yu
authors.initials: XY
authors.lastname: Sun
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Gu
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qiu-ping
authors.initials: QP
authors.lastname: Ding
authors.forename: Yi-fan
authors.initials: YF
authors.lastname: Zheng
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Xin-qiang
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects; D002244:Carbon / Q000008:administration & dosage / Q000494:pharmacology*; D006487:Hemostasis / Q000187:drug effects*; D008297:Male; D049329:Nanostructures; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation / Q000187:drug effects*; D010976:Platelet Count; D011517:Prothrombin Time; D051381:Rats; D017207:Rats, Sprague-Dawley; D013918:Thrombin Time
chemical_list: D002244:Carbon