title: Elimination of chloramphenicol in rainbow trout receiving medicated feed.
abstract: Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg-1d-1 of chloramphenicol for four days. Samples were taken one day before treatment and for 43 days after the treatment was over. Chloramphenicol was analysed using an in-house enzyme linked immunoassay (ELISA) validated against the criteria of the Commission Decision 2002/657/EC. Validation parameters confirmed that the method was appropriate for the detection of chloramphenicol at levels below the minimum required performance limit (MRPL) of 0.3 μg kg-1. The highest chloramphenicol levels were observed on the first day after the treatment had ended (144.3 μg kg-1). Elimination was significant over the first seven days; significant differences were detected between days 1 and 3 (p<0.001), 3 and 5 (p<0.001), and 5 and 7 (p<0.05). Chloramphenicol levels dropped below MRPL to 0.17 μg kg-1 on day 9 after the end of treatment. From day 11 to 43, chloramphenicol residues were detectable in a range from 0.091 μg kg-1 (highest) to 0.011 μg kg-1 (lowest). Our results indicate that trout muscle tissue could be compliant with health requirements for consumption 10 days after withdrawal from chloramphenicol treatment.
authors.affiliation: Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb. bilandzic@veinst.hr
authors.forename: Nina
authors.initials: N
authors.lastname: Bilandžić
authors.forename: Ivana
authors.initials: I
authors.lastname: Varenina
authors.forename: Sanin
authors.initials: S
authors.lastname: Tanković
authors.forename: Božica Solomun
authors.initials: BS
authors.lastname: Kolanović
mesh_terms: D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002701:Chloramphenicol / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004350:Drug Residues / Q000032:analysis; D018482:Muscle, Skeletal / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol