title: Human yeast-specific CD8 T lymphocytes show a nonclassical effector molecule profile.
abstract: Pathogenic yeast and fungi represent a major group of human pathogens. The consequences of infections are diverse and range from local, clinically uncomplicated mycosis of the skin to systemic, life-threatening sepsis. Despite extensive MHC class I-restricted frequencies of yeast-specific CD8 T lymphocytes in healthy individuals and the essential role of the cell-mediated immunity in controlling infections, the characteristics and defense mechanisms of antifungal effector cells are still unclear. Here, we describe the direct analysis of yeast-specific CD8 T lymphocytes in whole blood from healthy individuals. They show a unique, nonclassical phenotype expressing granulysin and granzyme K in lytic granules instead of the major effector molecules perforin and granzyme B. After stimulation in whole blood, yeast-specific CD8 T cells degranulated and, upon cultivation in the presence of IL-2, their granula were refilled with granulysin rather than with perforin and granzyme B. Moreover, yeast-specific stimulation through dendritic cells but not by yeast cells alone led to degranulation of the effector cells. As granulysin is the only effector molecule in lytic granules known to have antifungal properties, our data suggest yeast-specific CD8 T cells to be a nonclassical effector population whose antimicrobial effector machinery seems to be tailor-made for the efficient elimination of fungi as pathogens.
authors.affiliation: Junior Research Group for Virology/Immunology, Saarland University, 66421, Homburg, Germany. breinig@cs.uni-saarland.de
authors.forename: Tanja
authors.initials: T
authors.lastname: Breinig
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Scheller
authors.forename: Birgit
authors.initials: B
authors.lastname: Glombitza
authors.forename: Frank
authors.initials: F
authors.lastname: Breinig
authors.forename: Andreas
authors.initials: A
authors.lastname: Meyerhans
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D015550:Cell Degranulation; D003594:Cytoplasmic Granules / Q000201:enzymology*; D003603:Cytotoxins / Q000032:analysis*; D053804:Granzymes / Q000032:analysis; D006805:Human Experimentation; D006801:Humans; D054353:Perforin / Q000032:analysis; D015003:Yeasts / Q000276:immunology*
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D003603:Cytotoxins; C075005:GNLY protein, human; D054353:Perforin; D053804:Granzymes