title: Economic comparison of diagnostic antibody production in perfusion stirred tank and in hollow fiber bioreactor processes.
abstract: The total operating costs of small-scale monoclonal antibody production were calculated for two different upstream options and general downstream procedure based on protein A chromatography. The upstream options were a spin-filter equipped stirred-tank bioreactor (STR) and a hollow fiber bioreactor (HFB). Both the bioreactors were operated in perfusion mode. The total operating costs of the processes were 6,900 €/g for STR option and 6,400 €/g for the HFB option. In the both systems, the costs were dominated by expenses derived from the downstream section (almost 80%) that was almost identical in the both systems. In the upstream section, the investment depreciation was the largest cost item. The lower total costs of the HFB option were a result of lower investment costs and more concentrated product that led into savings also in downstream section. This study brings out the HFB as on viable alternative for stirred-tank bioreactor, especially in small-scale diagnostic monoclonal antibody production.
authors.affiliation: Dept. of Biotechnology and Chemical Technology, Aalto University School of Chemical Technology, Aalto, Finland. raisa.vermasvuori@aalto.fi
authors.forename: Raisa
authors.initials: R
authors.lastname: Vermasvuori
authors.forename: Markku
authors.initials: M
authors.lastname: Hurme
mesh_terms: D000911:Antibodies, Monoclonal / Q000191:economics* / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D019149:Bioreactors / Q000191:economics*; D001709:Biotechnology / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal