title: Effect of point-of-care CD4 cell count tests on retention of patients and rates of antiretroviral therapy initiation in primary health clinics: an observational cohort study.
abstract: BACKGROUND
  Loss to follow-up of HIV-positive patients before initiation of antiretroviral therapy can exceed 50% in low-income settings and is a challenge to the scale-up of treatment. We implemented point-of-care counting of CD4 cells in Mozambique and assessed the effect on loss to follow-up before immunological staging and treatment initiation.
  
  
  METHODS
  In this observational cohort study, data for enrolment into HIV management and initiation of antiretroviral therapy were extracted retrospectively from patients' records at four primary health clinics providing HIV treatment and point-of-care CD4 services. Loss to follow-up and the duration of each preparatory step before treatment initiation were measured and compared with baseline data from before the introduction of point-of-care CD4 testing.
  
  
  RESULTS
  After the introduction of point-of-care CD4 the proportion of patients lost to follow-up before completion of CD4 staging dropped from 57% (278 of 492) to 21% (92 of 437) (adjusted odds ratio [OR] 0·2, 95% CI 0·15-0·27). Total loss to follow-up before initiation of antiretroviral treatment fell from 64% (314 of 492) to 33% (142 of 437) (OR 0·27, 95% CI 0·21-0·36) and the proportion of enrolled patients initiating antiretroviral therapy increased from 12% (57 of 492) to 22% (94 of 437) (OR 2·05, 95% CI 1·42-2·96). The median time from enrolment to antiretroviral therapy initiation reduced from 48 days to 20 days (p<0·0001), primarily because of a reduction in the median time taken to complete CD4 staging, which decreased from 32 days to 3 days (p<0·0001). Loss to follow-up between staging and antiretroviral therapy initiation did not change significantly (OR 0·84, 95% CI 0·49-1·45).
  
  
  CONCLUSIONS
  Point-of-care CD4 testing enabled clinics to stage patients rapidly on-site after enrolment, which reduced opportunities for pretreatment loss to follow-up. As a result, more patients were identified as eligible for and initiated antiretroviral treatment. Point-of-care testing might therefore be an effective intervention to reduce pretreatment loss to follow-up.
  
  
  BACKGROUND
  Absolute Return for Kids and UNITAID.
authors.affiliation: Instituto Nacional da Saúde, Maputo, Mozambique. ilesh.jani@gmail.com
authors.forename: Ilesh V
authors.initials: IV
authors.lastname: Jani
authors.forename: Nádia E
authors.initials: NE
authors.lastname: Sitoe
authors.forename: Eunice R
authors.initials: ER
authors.lastname: Alfai
authors.forename: Patrina L
authors.initials: PL
authors.lastname: Chongo
authors.forename: Jorge I
authors.initials: JI
authors.lastname: Quevedo
authors.forename: Beatriz M
authors.initials: BM
authors.lastname: Rocha
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Lehe
authors.forename: Trevor F
authors.initials: TF
authors.lastname: Peter
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000553:Ambulatory Care / Q000379:methods*; D023241:Antiretroviral Therapy, Highly Active / Q000379:methods*; D001294:Attitude to Health; D018791:CD4 Lymphocyte Count; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D016001:Confidence Intervals; D003906:Developing Countries; D005260:Female; D005500:Follow-Up Studies; D015658:HIV Infections / Q000188:drug therapy* / Q000276:immunology; D006801:Humans; D007223:Infant; D008297:Male; D009073:Mozambique; D016017:Odds Ratio; D010349:Patient Compliance; D019095:Point-of-Care Systems; D012189:Retrospective Studies; D018570:Risk Assessment; D012737:Sex Factors; D012959:Socioeconomic Factors; D016896:Treatment Outcome; D055815:Young Adult