title: The presence of three repeats in the 5' UTR region of thymidylate synthase             (TS) is associated with increased TS mRNA expression in cultured human cancer             cell lines in vitro.
abstract: Thymidylate synthase (TS) gene contains 28-bp polymorphic sequence and 6-bp             insert at the 5'- and 3'-untranslated region, respectively. We investigated the             role of these two polymorphic traits on TS mRNA expression in nine different cultured             human cancer cell lines in vitro. Three cell lines each were 2R/2R, 2R/3R and             3R/3R genotypes. Six of the nine cell lines tested homozygous for the presence             of 6-bp insert (+6/+6) and the rest three lacked this insert (-6/-6). TS expression             analyses associated homozygous three repeats (3R/3R) to higher TS expression.
authors.affiliation: Department of Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors.forename: Hiba
authors.initials: H
authors.lastname: Hammad
authors.forename: Malancha
authors.initials: M
authors.lastname: Sarkar
authors.forename: Nikhil
authors.initials: N
authors.lastname: Gupta
authors.forename: Bach
authors.initials: B
authors.lastname: Ardalan
authors.forename: Pochi R
authors.initials: PR
authors.lastname: Subbarayan
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics*; D001483:Base Sequence; D045744:Cell Line, Tumor; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000235:genetics*; D005838:Genotype; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D013940:Thymidylate Synthase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger; D013940:Thymidylate Synthase