title: Reactions of metallodrugs with proteins: selective binding of phosphane-based platinum(II) dichlorides to horse heart cytochrome c probed by ESI MS coupled to enzymatic cleavage.
abstract: Reactions of cytotoxic platinum drugs with proteins are attracting growing attention for their relevant biological implications. We report here on the reactions of two cis-diphosphane platinum(II) dichlorides (namely cis-bis(trimethylphosphane) platinum(II) dichloride and cis-bis(triethylphosphane) platinum(II) dichloride) with horse heart cytochrome c (cyt c) monitored through advanced ESI MS methods coupled to enzymatic digestion. A remarkable selectivity in terms of adduct stoichiometry is highlighted and the specific metal binding sites are localised on the protein surface.
authors.affiliation: Institute of Inorganic and Analytical Chemistry, Friedrich-Schiller-University Jena, Humboldstrasse 8, 07743 Jena, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Mügge
authors.forename: Elena
authors.initials: E
authors.lastname: Michelucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Boscaro
authors.forename: Chiara
authors.initials: C
authors.lastname: Gabbiani
authors.forename: Luigi
authors.initials: L
authors.lastname: Messori
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Weigand
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001665:Binding Sites; D002712:Chlorides / Q000737:chemistry / Q000494:pharmacology; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism*; D006736:Horses; D009206:Myocardium / Q000201:enzymology; D017671:Platinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D011485:Protein Binding; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000970:Antineoplastic Agents; D002712:Chlorides; D017671:Platinum Compounds; D045304:Cytochromes c