title: Effect of bacoside extract from Bacopa monniera on physical fatigue induced by forced swimming.
abstract: The antifatigue effect of bacoside extract (BME) from Bacopa monniera (L.) Wettst. was investigated. Rats were subjected to weight-loaded forced swim test (WFST) every alternate day for 3 weeks. The BME at a dosage of 10 mg/kg body weight was administered orally to rats for 2 weeks in order to evaluate the following biomarkers of physical fatigue: swimming time, change in body weight, lipid peroxidation, lactic acid (LA), glycogen, antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) and blood parameters, namely blood urea nitrogen (BUN) and creatine kinase (CK). The exhaustive swimming time was increased by 3-fold in the BME supplemented group compared with that of the control group on day 13. The BME treatment lowered malondialdehyde (MDA) levels in brain, liver and muscle tissues by 11.2%, 16.2% and 37.7%, respectively, compared with the control exercised group (p < 0.05). The BME also reduced the LA, serum BUN and CK activities significantly compared with that of the control. Administration of BME significantly protected the depletion of SOD and CAT activities. The HSP-70 expression studies by western blot also confirmed the antifatigue property of BME. The present study thus indicates that BME ameliorates the various impairments associated with physical fatigue.
authors.affiliation: Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore 570011, India. ananddfrl@yahoo.com
authors.forename: T
authors.initials: T
authors.lastname: Anand
authors.forename: G
authors.initials: G
authors.lastname: Phani Kumar
authors.forename: M D
authors.initials: MD
authors.lastname: Pandareesh
authors.forename: M S L
authors.initials: MS
authors.lastname: Swamy
authors.forename: Farhath
authors.initials: F
authors.lastname: Khanum
authors.forename: A S
authors.initials: AS
authors.lastname: Bawa
mesh_terms: D000818:Animals; D034681:Bacopa / Q000737:chemistry*; D015415:Biomarkers / Q000737:chemistry; D001806:Blood Urea Nitrogen; D015153:Blotting, Western; D001835:Body Weight; D001921:Brain / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation / Q000187:drug effects; D005221:Fatigue / Q000188:drug therapy* / Q000473:pathology; D006003:Glycogen / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013550:Swimming; D013997:Time Factors
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D010936:Plant Extracts; D019344:Lactic Acid; D008315:Malondialdehyde; D006003:Glycogen; D002374:Catalase; D013482:Superoxide Dismutase