title: Evaluation of the immunogenicity and in vivo toxicity of the antimicrobial peptide P34.
abstract: Immunogenicity and toxicity of antimicrobial peptide P34 were evaluated in vivo. BALB/c mice were inoculated intraperitoneally with peptide P34 alone and associated with Freund's adjuvant. For acute toxicity testing, different concentrations of the peptide P34 (82.5, 165.0, 247.5 and 330.0mg/kg) were orally administered. To evaluate the sub-chronic toxicity the tested dose of 0.825 mg/kg/day of the peptide P34 or nisin were administered for 21 days. There were no hypersensitivity reactions or significant increase in antibody titer during the immunogenicity experiment or death of animals during the acute or sub-chronic toxicity tests. The LD(50) was higher than 332.3 ± 0.76 mg/kg. No significant changes in serum biochemical parameters were observed in the animals treated with the peptide P34 unlike nisin-treated group showed a significant increase in alanine transaminase levels in comparison to controls. The group treated with 0.825 mg/kg/day of nisin showed histological changes in the spleen, skin and liver. In the group treated with peptide P34 histological changes in the spleen were observed, with the presence of megakaryocytes. Few studies report the use of animal models to evaluate the in vivo toxicity of antimicrobial peptides and such investigation is an essential step to ensure it safe use in foods.
authors.affiliation: Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: Rodrigo de Almeida
authors.initials: Rde A
authors.lastname: Vaucher
authors.forename: Camila de Campos Velho
authors.initials: Cde C
authors.lastname: Velho Gewehr
authors.forename: Ana Paula Folmer
authors.initials: AP
authors.lastname: Correa
authors.forename: Voltaire
authors.initials: V
authors.lastname: Sant'Anna
authors.forename: Juliano
authors.initials: J
authors.lastname: Ferreira
authors.forename: Adriano
authors.initials: A
authors.lastname: Brandelli
mesh_terms: D000818:Animals; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009561:Nisin / Q000633:toxicity*; D010455:Peptides / Q000008:administration & dosage / Q000633:toxicity*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013154:Spleen / Q000187:drug effects / Q000473:pathology
chemical_list: C549460:BLS P34 peptide, Bacillus; D010455:Peptides; D009561:Nisin; D005620:Freund's Adjuvant