title: A molecular mechanism of direction switching in the flagellar motor of Escherichia coli.
abstract: The direction of flagellar rotation is regulated by a rotor-mounted protein assembly, termed the "switch complex," formed from multiple copies of the proteins FliG, FliM, and FliN. The structures of major parts of these proteins are known, and the overall organization of proteins in the complex has been elucidated previously using a combination of protein-binding, mutational, and cross-linking approaches. In Escherichia coli, the switch from counterclockwise to clockwise rotation is triggered by the signaling protein phospho-CheY, which binds to the lower part of the switch complex and induces small movements of FliM and FliN subunits relative to each other. Direction switching also must produce movements in the upper part of the complex, particularly in the C-terminal domain of FliG (FliG(C)), which interacts with the stator to generate the torque for flagellar rotation. In the present study, protein movements in the middle and upper parts of the switch complex have been probed by means of targeted cross-linking and mutational analysis. Switching induces a tilting movement of the FliM domains that form the middle part of the switch and a consequent rotation of the affixed FliG(C) domains that reorients the stator interaction sites by about 90°. In a recently proposed hypothesis for the motor mechanism, such a reorientation of FliG(C) would reverse the direction of motor rotation.
authors.affiliation: Department of Biology, University of Utah, Salt Lake City, UT 84112, USA.
authors.forename: Koushik
authors.initials: K
authors.lastname: Paul
authors.forename: Duncan
authors.initials: D
authors.lastname: Brunstetter
authors.forename: Sienna
authors.initials: S
authors.lastname: Titen
authors.forename: David F
authors.initials: DF
authors.lastname: Blair
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005407:Flagella / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D000072236:Methyl-Accepting Chemotaxis Proteins; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D008958:Models, Molecular; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009068:Movement / Q000502:physiology; D017434:Protein Structure, Tertiary; D012399:Rotation
chemical_list: D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D029968:Escherichia coli Proteins; C077389:FliN protein, Bacteria; C075660:Flig protein, Bacteria; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; D020409:Molecular Motor Proteins; C000607606:cheY protein, E coli; C066821:FliM protein, Bacteria