title: Fine mapping the number of corpora lutea quantitative trait loci on SSC3: analysis of the porcine follicle-stimulating hormone receptor gene.
abstract: In females, follicle-stimulating hormone (FSH) targets a FSH receptor (FSHR) expressed only on granulose cells, inducing maturation of the ovarian follicles. We hypothesized that genetic variants in the FSHR gene influence litter size by affecting the number of corpora lutea. We fine-mapped a region of Sus Scrofa chromosome 3 that contains quantitative trait loci for corpora lutea. Polymorphisms were detected in the exons and 5' flanking region of the porcine FSHR gene, a positional candidate for the statistically most significant of the quantitative trait loci. Finally, 248 F(2) animals from a Duroc and Meishan cross were genotyped for three FSHR SNPs at positions 74, 532 and 1166, and these were correlated with the phenotypes of litter size and corpus luteum number. Three haplotypes were identified: M1 (G/G/C), M2 (C/A/T) and D (C/A/C). In the F(2) population, the M1 haplotype was associated with a greater number of corpora lutea (P < 0.01) and also seemed to be associated with increased litter size, although the association was not significant (P = 0.2571). Some polymorphisms resulting in amino acid substitutions in these genes were excluded from the polymorphisms possibly responsible for the number of corpora lutea.
authors.affiliation: National Livestock Breeding Center, Nishigo, Fukushima National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan. s0sato@nlbc.go.jp
authors.forename: Shuji
authors.initials: S
authors.lastname: Sato
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hayashi
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003338:Corpus Luteum; D005260:Female; D005787:Gene Frequency; D006239:Haplotypes; D008098:Litter Size / Q000235:genetics*; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci; D011962:Receptors, FSH / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics* / Q000502:physiology*
chemical_list: D011962:Receptors, FSH