title: Effects of marmin, a compound isolated from Aegle marmelos Correa, on contraction of the guinea pig-isolated trachea.
abstract: Marmin or 7-(6',7'-dihydroxygeranyl-oxy)coumarin is a compound isolated from Aegle marmelos Correa. In the study, we examined the effects of marmin on the contraction of guinea pig-isolated trachea stimulated by several inducers, namely histamine, metacholine, compound 48/80. We also evaluated its action against contraction induced by extracellular or intracellular calcium ion. The possibility of marmin to potentiate the relaxation effect of isoprenaline was also studied. Marmin added in the organ bath at 10 min prior to the agonist inhibited the contraction elicited by histamine and metacholine in a concentration-dependent manner. Moreover, marmin antagonized the histamine-induced contraction in competitive manner. Marmin mildly potentiated the relaxation effect of isoprenaline. In the study, marmin abrogated the contraction of tracheal smooth muscle induced by compound 48/80, an inducer of histamine release. Besides, marmin successfully inhibited CaCl(2)-induced contraction in Ca(2+)-free Krebs solution. Marmin also inhibited two phases of contraction which were consecutively induced by metacholine and CaCl(2) in Ca(2+)-free Krebs solution. Based on the results we concluded that marmin could inhibit contraction of the guinea-pig tracheal smooth muscle, especially by interfering histamine receptor, inhibiting the histamine release from mast, inhibiting intracellular Ca(2+) release from the intracellular store and the Ca(2+) influx through voltage-dependent Ca(2+) channels.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia. agungendronugroho@yahoo.com
authors.forename: Agung Endro
authors.initials: AE
authors.lastname: Nugroho
authors.forename: Yance
authors.initials: Y
authors.lastname: Anas
authors.forename: Puguh Novi
authors.initials: PN
authors.lastname: Arsito
authors.forename: Joko Tri
authors.initials: JT
authors.lastname: Wibowo
authors.forename: Sugeng
authors.initials: S
authors.lastname: Riyanto
authors.forename: Mohamad Aspollah
authors.initials: MA
authors.lastname: Sukari
mesh_terms: D045471:Aegle / Q000737:chemistry*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D003374:Coumarins / Q000302:isolation & purification / Q000494:pharmacology*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects* / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D014132:Trachea / Q000187:drug effects* / Q000502:physiology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D003374:Coumarins; D016210:Methacholine Chloride; C092088:marmin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D007545:Isoproterenol; D002122:Calcium Chloride