title: Estimation of the lipophilic character of flavonoids from the retention behavior in reversed phase liquid chromatography on different stationary phases: A comparative study.
abstract: The retention behavior of some flavonoids in reversed phase liquid chromatography (RPLC) was investigated using different chemistries of the modified silicagel based stationary phases. Highly end-capped octadecyl silicagel (ODS), polar embedded linker octadecyl silicagel (SB-18 Aqua), phenyl silicagel and pentafluorophenyl modified silicagel (PFP) were used as stationary phases. The mobile phase consisted in acetonitrile/acidified water mixtures, at different fractions of volume. The lipophilicity was estimated through different chromatographic descriptors, as it follows: log k(w), m log k, S, φ(0) and PC1/log k. The chromatographic behavior observed on the mentioned stationary phases was evaluated by means of various graphical profiles and correlation matrices. Additionally, new information about the characteristics of the stationary phases and their (dis)-similarities were provided through lipophilicity charts and by scatterplots of loadings obtained by applying principal component analysis (PCA) to retention data. Furthermore, the experimental lipophilicity indices estimated from retention data were correlated with the computed descriptors, at a high level of statistical significance.
authors.affiliation: University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, Panduri Av. # 90, Bucharest 050663, Romania.
authors.forename: Florentin
authors.initials: F
authors.lastname: Tache
authors.forename: Rodica Domnica
authors.initials: RD
authors.lastname: Naşcu-Briciu
authors.forename: Costel
authors.initials: C
authors.lastname: Sârbu
authors.forename: Florina
authors.initials: F
authors.lastname: Micăle
authors.forename: Andrei
authors.initials: A
authors.lastname: Medvedovici
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry*; D025341:Principal Component Analysis
chemical_list: D005419:Flavonoids