title: Screening for microbial metabolites affecting phenotype of Caenorhabditis elegans.
abstract: Microbial samples, including our library of known microbial compounds (ca. 300) and microbial culture broths (ca. 9000), were screened for small molecules affecting the phenotype of Caenorhabditis elegans. As a result, seven known compounds were found to induce phenotypic abnormality of C. elegans. Staurosporine exhibited morphological defects in the vulva and tail of C. elegans, avermectin B1a exhibited hatching inhibition of starting eggs on day 1 at 25-100 µM and growth inhibition at 0.01-12.5 µM, siccanin and antimycin A inhibited the growth of C. elegans, and fluorouracil inhibited hatching of eggs newly spawned by adult C. elegans. Toromycin induced morphological defects in the intestine. 5-(4-Methoxyphenyl)-oxazole, isolated as a fungal metabolite for the first time, inhibited the hatching of eggs newly spawned by adult C. elegans.
authors.affiliation: Graduate School of Pharmaceutical Science, Kitasato University, Tokyo 108–8641, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamamuro
authors.forename: Ryuji
authors.initials: R
authors.lastname: Uchida
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Rokuro
authors.initials: R
authors.lastname: Masuma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology; D005260:Female; D005472:Fluorouracil / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007559:Ivermectin / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D023421:Models, Animal; D009154:Mutation; D010641:Phenotype; D054852:Small Molecule Libraries / Q000032:analysis / Q000378:metabolism; D013623:Tail / Q000033:anatomy & histology; D014844:Vulva / Q000033:anatomy & histology; D015053:Zygote / Q000378:metabolism / Q000473:pathology / Q000502:physiology*
chemical_list: D000969:Antinematodal Agents; D029742:Caenorhabditis elegans Proteins; D054852:Small Molecule Libraries; C022120:avermectin B(1)a; D007559:Ivermectin; D005472:Fluorouracil