title: Modulation of CYP1A and genotoxic effects in European seabass (Dicentrarchus labrax) exposed to weathered oil: a mesocosm study.
abstract: The aim of this study was to assess medium-term toxicity of weathered oil on European seabass. A mesocosm system reproducing an oil spill at sea was applied. Fish were collected after 48 h, 7, 30 and 60 days. Cyp1a gene transcription, EROD and UDPGT activities, bile PAHs metabolites and micronuclei frequency were investigated. A progressive disappearance of low molecular weight n-alkanes and PAHs in the water of the mesocosm occurred during the experimentation. Fishes exposed to oil displayed a significant increase of cyp1a expression and EROD activity during the entire experiment as well as higher concentrations of PAHs metabolites in bile. Micronulei frequency resulted significantly higher during all experiment in oil exposed sea bass compared to controls. The results highlight the environmental risk associated with the release of oil products at sea and confirm the adopted parameters as useful tools for studying the impact of accidental oil spills on fish.
authors.affiliation: Department of Environmental Sciences "G. Sarfatti", University of Siena, Siena, Italy. dellatorre2@unisi.it
authors.forename: Camilla
authors.initials: C
authors.lastname: Della Torre
authors.forename: Andrea
authors.initials: A
authors.lastname: Tornambè
authors.forename: Simone
authors.initials: S
authors.lastname: Cappello
authors.forename: Michela
authors.initials: M
authors.lastname: Mariottini
authors.forename: Guido
authors.initials: G
authors.lastname: Perra
authors.forename: Silvia
authors.initials: S
authors.lastname: Giuliani
authors.forename: Ezio
authors.initials: E
authors.lastname: Amato
authors.forename: Carla
authors.initials: C
authors.lastname: Falugi
authors.forename: Antonella
authors.initials: A
authors.lastname: Crisari
authors.forename: Michail M
authors.initials: MM
authors.lastname: Yakimov
authors.forename: Erika
authors.initials: E
authors.lastname: Magaletti
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001492:Bass / Q000235:genetics* / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects*; D004781:Environmental Exposure; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D014453:Glucuronosyltransferase / Q000378:metabolism; D010578:Petroleum / Q000378:metabolism / Q000633:toxicity*; D013997:Time Factors; D000090264:UDP-Glucuronosyltransferase 1A9; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D010578:Petroleum; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1; D014453:Glucuronosyltransferase; D000090264:UDP-Glucuronosyltransferase 1A9