title: The effects of the intraperitoneal administration of oxaliplatin and 5-FU on the healing of colonic anastomoses: an experimental study.
abstract: BACKGROUND
  The purpose of this experimental study was to assess the effects of the immediate postoperative intraperitoneal administration of oxaliplatin and 5-FU on the healing of colonic anastomoses in rats.
  
  
  METHODS
  Sixty rats were randomized into 4 groups of 15 rats each and were subjected to colonic anastomoses. To the 1st group, saline solution was administered immediately postoperatively, intraperitoneally. To the 2nd group, 5-FU was administered, to the 3rd group oxaliplatin and to the 4th group 5-FU and oxaliplatin were administered immediately postoperatively, intraperitoneally. After killing the rats on the 8th postoperative day, the anastomoses were examined macroscopically and the anastomotic bursting pressures were measured. The anastomoses were also examined histologically and the hydroxyproline contents were determined.
  
  
  RESULTS
  Rupture of the anastomosis was observed in no rats of the 1st group, in 3 rats of the 2nd group, in 4 rats of the 3rd group and in 7 rats of the 4th group (P = 0.016). The bursting pressure (P < 0.001), the hydroxyproline content (P < 0.001) and the concentration of collagen (P < 0.001) and fibroblasts (P < 0.001) were significantly lower in the 2nd, 3rd and 4th group in comparison with the 1st group. The formation of adhesions and the leukocytosis on the anastomoses were significantly higher in the 2nd, 3rd and 4th group than in the 1st group (P < 0.001).
  
  
  CONCLUSIONS
  The immediate postoperative, intraperitoneal administration of oxaliplatin, 5-FU or the combination of 5-FU and oxaliplatin impairs the healing of colonic anastomoses in rats.
authors.affiliation: Fourth Surgical Department, Aristotle University of Thessaloniki, 1 Antheon str, Panorama, 55236 Thessaloniki, Greece.
authors.forename: D
authors.initials: D
authors.lastname: Kanellos
authors.forename: M G
authors.initials: MG
authors.lastname: Pramateftakis
authors.forename: I
authors.initials: I
authors.lastname: Mantzoros
authors.forename: E
authors.initials: E
authors.lastname: Zacharakis
authors.forename: D
authors.initials: D
authors.lastname: Raptis
authors.forename: K
authors.initials: K
authors.lastname: Despoudi
authors.forename: Th
authors.initials: T
authors.lastname: Zaraboukas
authors.forename: G
authors.initials: G
authors.lastname: Koliakos
authors.forename: H
authors.initials: H
authors.lastname: Lazaridis
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000009:adverse effects / Q000494:pharmacology*; D003106:Colon / Q000098:blood supply / Q000737:chemistry / Q000473:pathology / Q000601:surgery*; D005472:Fluorouracil / Q000009:adverse effects / Q000494:pharmacology*; D006909:Hydroxyproline / Q000032:analysis / Q000187:drug effects; D007964:Leukocytosis / Q000209:etiology; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009944:Organoplatinum Compounds / Q000009:adverse effects / Q000494:pharmacology*; D000077150:Oxaliplatin; D011312:Pressure / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012421:Rupture / Q000209:etiology; D000267:Tissue Adhesions / Q000209:etiology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D006909:Hydroxyproline; D005472:Fluorouracil