title: Site-specific incorporation of unnatural amino acids as probes for protein conformational changes.
abstract: Site-specific in vivo incorporation of unnatural amino acids provides powerful tools for the study of protein interaction and dynamics. Here, we provide a protocol for the incorporation of six such UAA probes into a GFP reporter system, expressed in Escherichia coli from both arabinose and lactose-inducible expression plasmids using an autoinduction media.
authors.affiliation: Department of Chemistry, Franklin & Marshall College, Lancaster, PA, USA.
authors.forename: Jennifer C
authors.initials: JC
authors.lastname: Peeler
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Mehl
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D015335:Molecular Probes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D015335:Molecular Probes; D049452:Green Fluorescent Proteins; D000604:Amino Acyl-tRNA Synthetases