title: Laser microstructuring of photomodified fluorinated ethylene propylene surface for confined growth of Chinese hamster ovary cells and single cell isolation.
abstract: We present a method for laser lithography of cell-adhesive arrays on a fluoropolymer surface. The method is based on 172 nm excimer-lamp photomodification in ammonia atmosphere followed by microstructuring by laser ablation. The improved wettability of the polymer is caused by new chemical groups on the surface after the UV treatment that we proved by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. The cell adhesion properties of micropatterned structures were tested by cultivation of mammalian cells. We show that single elongated cells can grow confined to lines with sharply defined boundaries of the cell-covered areas. In preliminary experiments, we also demonstrate that the described technique allows the production of single-cell arrays with variable cell shape.
authors.affiliation: Institute of Applied Physics, Johannes Kepler University Linz, Linz, Austria. sergii.yakunin@jku.at
authors.forename: S
authors.initials: S
authors.lastname: Yakunin
authors.forename: M
authors.initials: M
authors.lastname: Fahrner
authors.forename: B
authors.initials: B
authors.lastname: Reisinger
authors.forename: H
authors.initials: H
authors.lastname: Itani
authors.forename: C
authors.initials: C
authors.lastname: Romanin
authors.forename: J
authors.initials: J
authors.lastname: Heitz
mesh_terms: D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D002469:Cell Separation; D006224:Cricetinae; D003412:Cricetulus; D005461:Fluorine / Q000737:chemistry*; D007834:Lasers; D046228:Microarray Analysis; D010777:Photochemistry / Q000379:methods; D020959:Polyethylene / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D014466:Ultraviolet Rays; D017155:Wettability
chemical_list: D011126:Polypropylenes; D005461:Fluorine; D020959:Polyethylene