title: Measurement of amide hydrogen exchange rates with the use of radiation damping.
abstract: A simple method for measuring amide hydrogen exchange rates is presented, which is based on the selective inversion of water magnetization with the use of radiation damping. Simulations show that accurate exchange rates can be measured despite the complications of radiation damping and cross relaxation to the exchange process between amide and water protons. This method cannot eliminate the contributions of the exchange-relayed NOE and direct NOE to the measured exchange rates, but minimize the direct NOE contribution. In addition, the amides with a significant amount of such indirect contributions are possible to be identified from the shape of the exchange peak intensity profiles or/and from the apparent relaxation rates of amide protons which are extracted from fitting the intensity profiles to an equation established here for our experiment. The method was tested on ubiquitin and also applied to an acyl carrier protein. The amide exchange rates for the acyl carrier protein at two pHs indicate that the entire protein is highly dynamic on the second timescale. Low protection factors for the residues in the regular secondary structural elements also suggest the presence of invisible unfolded species. The highly dynamic nature of the acyl carrier protein may be crucial for its interactions with its substrate and enzymes.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
authors.forename: Jing-Song
authors.initials: JS
authors.lastname: Fan
authors.forename: Jackwee
authors.initials: J
authors.lastname: Lim
authors.forename: Binhan
authors.initials: B
authors.lastname: Yu
authors.forename: Daiwen
authors.initials: D
authors.lastname: Yang
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry*; D000577:Amides / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017510:Protein Folding; D011522:Protons; D014867:Water / Q000737:chemistry
chemical_list: D000213:Acyl Carrier Protein; D000577:Amides; D011522:Protons; D014867:Water; D006859:Hydrogen