title: ELISPOT assays to enumerate bovine IFN-γ-secreting cells for the development of novel vaccines against bovine tuberculosis.
abstract: Enumeration of antigen-specific cells after vaccination is one of the prime immunological parameters determined when developing vaccines. Due to their exquisite sensitivity (limits of detection can be below 1/100,000 cells), ELISPOT assays are therefore an important tool in vaccine development programs. This is particularly the case for vaccines against diseases that require protective cell-mediated immunity, such as tuberculosis. This chapter describes ELISPOT assays detecting bovine IFN-γ.
authors.affiliation: Department of Bacteriology, TB Research Group, Veterinary Laboratories Agency, WeybridgeAddlestone, Surrey, UK. m.vordermeier@vla.defra.gsi.gov.uk
authors.forename: Martin
authors.initials: M
authors.lastname: Vordermeier
authors.forename: Adam O
authors.initials: AO
authors.lastname: Whelan
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D058501:Enzyme-Linked Immunospot Assay; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014380:Tuberculosis, Bovine / Q000276:immunology* / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
chemical_list: D032581:Tuberculosis Vaccines; D007371:Interferon-gamma