title: Diagnosis of paratuberculosis in goats by cell mediated immune response, conventional and molecular diagnostic techniques.
abstract: In the present study efficacy of single intradermal Johnin test, acid fast staining of faecal smear and IS 900 faecal polymerase chain reaction tests was evaluated in 200 goats for detection of Mycobacterium avium subsp paratuberculosis. Two hundred goats comprising 150 goats from an organised farm in Trichur district and 50 goats reared under field condition at farmers premise from Malappuram district of Kerala state formed the study population. Faecal smear from all the 200 goats was stained by Ziehl-Neelsen acid fast stain and faecal polymerase chain reaction (PCR) specific for M. avium subsp paratuberculosis (MAP); IS 900 was performed on all samples. All the animals were subjected to single intradermal Johnin test. Out of 200 goats screened for paratuberculosis, six goats (3%), 11 goats (5.5%) and 42 goats (21%) were found positive by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS 900 PCR respectively. Results of the present study indicate that amplification of IS 900 insertion element was the most specific and sensitive diagnostic detection method. Single intradermal Johnin test and Ziehl-Neelsen acid fast staining did not show any significant difference.
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Trichur, Kerala, India. drsulficars@gmail.com
authors.forename: Sulficar Shamsudeen
authors.initials: SS
authors.lastname: Rawther
authors.forename: Mapranath Raghavan
authors.initials: MR
authors.lastname: Saseendranath
authors.forename: Govinda Pilla Krishnan
authors.initials: GP
authors.lastname: Nair
authors.forename: Palathingal Vareid
authors.initials: PV
authors.lastname: Tresamol
authors.forename: Usha Narayana
authors.initials: UN
authors.lastname: Pillai
authors.forename: John
authors.initials: J
authors.lastname: Abraham
authors.forename: Tuticorin Maragatham Alagesan
authors.initials: TM
authors.lastname: Senthilkumar
authors.forename: Shrine
authors.initials: S
authors.lastname: Nagalakshmy
authors.forename: Krishna Kripa Narayanan
authors.initials: KK
authors.lastname: Nimisha
mesh_terms: D000818:Animals; D005243:Feces / Q000382:microbiology; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D006041:Goats; D007194:India; D007428:Intradermal Tests / Q000662:veterinary; D008297:Male; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000662:veterinary