title: Bubble cell for magnetic bead trapping in capillary electrophoresis.
abstract: A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case with magnetic beads trapped in a standard capillary, thereby having less effect on the experimental conditions. Using numerical simulations and microscopic visualizations, the capture of beads inside a bubble cell was investigated with two magnet configurations. Pressure-driven and electro-osmotic flow velocities were measured for different amounts of protein-A-coated beads or C18-functionalized beads (RPC-18). Solid-phase extraction of a model antibody on protein-A beads and preconcentration of fluorescein on RPC-18 beads were performed as proof of concept experiments.
authors.affiliation: Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: Gassner
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Proczek
authors.forename: Hubert H
authors.initials: HH
authors.lastname: Girault
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D008280:Magnetics; D011485:Protein Binding; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000737:chemistry
chemical_list: D000906:Antibodies; D013205:Staphylococcal Protein A