title: Site-Specific Insertion Polymorphism of the MITE Alex-1 in the Genus Coffea Suggests Interspecific Gene Flow.
abstract: Miniature Inverted-repeat Transposable Elements (MITEs) are small nonautonomous class-II transposable elements distributed throughout eukaryotic genomes. We identified a novel family of MITEs (named Alex) in the Coffea canephora genome often associated with expressed sequences. The Alex-1 element is inserted in an intron of a gene at the CcEIN4 locus. Its mobility was demonstrated by sequencing the insertion site in C. canephora accessions and Coffea species. Analysis of the insertion polymorphism of Alex-1 at this locus in Coffea species and in C. canephora showed that there was no relationship between the geographical distribution of the species, their phylogenetic relationships, and insertion polymorphism. The intraspecific distribution of C. canephora revealed an original situation within the E diversity group. These results suggest possibly greater gene flow between species than previously thought. This MITE family will enable the study of the C. canephora genome evolution, phylogenetic relationships, and possible gene flows within the Coffea genus.
authors.affiliation: IRD, UMR DIADE, Centre IRD de Montpellier, BP 64501, 34394 Montpellier Cedex 5, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Dubreuil-Tranchant
authors.forename: Romain
authors.initials: R
authors.lastname: Guyot
authors.forename: Amira
authors.initials: A
authors.lastname: Guellim
authors.forename: Caroline
authors.initials: C
authors.lastname: Duret
authors.forename: Marion
authors.initials: M
authors.lastname: de la Mare
authors.forename: Norosoa
authors.initials: N
authors.lastname: Razafinarivo
authors.forename: Valérie
authors.initials: V
authors.lastname: Poncet
authors.forename: Serge
authors.initials: S
authors.lastname: Hamon
authors.forename: Perla
authors.initials: P
authors.lastname: Hamon
authors.forename: Alexandre
authors.initials: A
authors.lastname: de Kochko