title: Microscope objectives.
abstract: The objective is the most crucial image-forming component of a microscope. A knowledge of the many types of objectives available and their characteristics is critical to the selection of appropriate objectives for image cytometry. This unit discusses aberrations in image formation and their correction, construction, and types of objectives, and objectives for other microscopy applications, explaining the advantages and limitations of each one.
authors.affiliation: Nikon Instruments, Melville, New York.
authors.forename: Joseph
authors.initials: J
authors.lastname: LoBiondo
authors.affiliation: Olympus America, Central Valley, Pennsylvania.
authors.forename: Mortimer
authors.initials: M
authors.lastname: Abramowitz
authors.affiliation: AccuMed International, Chicago, Illinois.
authors.forename: Marc M
authors.initials: MM
authors.lastname: Friedman
mesh_terms: D000818:Animals; D006801:Humans; D021621:Imaging, Three-Dimensional; D008027:Light; D008853:Microscopy / Q000295:instrumentation*; D012031:Refractometry