title: Resveratrol modulates MED28 (Magicin/EG-1) expression and inhibits epidermal growth factor (EGF)-induced migration in MDA-MB-231 human breast cancer cells.
abstract: Resveratrol and pterostilbene exhibit diverse biological activities. MED28, a subunit of the mammalian Mediator complex for transcription, was also identified as magicin, an actin cytoskeleton Grb2-associated protein, and as endothelial-derived gene (EG-1). Several tumors exhibit aberrant MED28 expression, whereas the underlying mechanism is unclear. Triple-negative breast cancers, often expressing epidermal growth factor (EGF) receptor (EGFR), are associated with metastasis and poor survival. The objective of this study is to compare the effect of resveratrol and pterostilbene and to investigate the role of MED28 in EGFR-overexpressing MDA-MB-231 breast cancer cells. Pretreatment of resveratrol, but not pterostlbene, suppressed EGF-mediated migration and expression of MED28 and matrix metalloproteinase (MMP)-9 in MDA-MB-231 cells. Moreover, overexpression of MED28 increased migration, and the addition of EGF further enhanced migration. Our data indicate that resveratrol modulates the effect of MED28 on cellular migration, presumably through the EGFR/phosphatidylinositol 3-kinase (PI3K) signaling pathway, in breast cancer cells.
authors.affiliation: Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan, Taiwan, ROC. leemf@mail.cjcu.edu.tw
authors.forename: Ming-Fen
authors.initials: MF
authors.lastname: Lee
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Pan
authors.forename: Yi-Siou
authors.initials: YS
authors.lastname: Chiou
authors.forename: An-Chin
authors.initials: AC
authors.lastname: Cheng
authors.forename: Han
authors.initials: H
authors.lastname: Huang
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D015536:Down-Regulation / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D056892:Mediator Complex / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: C496877:MED28 protein, human; D056892:Mediator Complex; D010936:Plant Extracts; D013267:Stilbenes; D004815:Epidermal Growth Factor; D000077185:Resveratrol