title: Inhibitory effect of two Indian medicinal plants on aldose reductase of rat lens in vitro.
abstract: OBJECTIVE
  To assesse the inhibitory effect of alcoholic extract of two Indian medicinal plants namely Ceasalpinia digyna Rottler and, Alangium lamarckii Thwaits on aldose reductase (AR) of rat lens.
  
  
  METHODS
  Rats lens were enucleated through posterior approach and their homogenate was prepared and centrifuged to obtain a clear supernatant for the determination of AR activity and protein content.
  
  
  RESULTS
  The alcoholic extract of Ceasalpinia digyna and Alangium lamarckii had a potent inhibitory effect on the lens AR enzyme. The IC(50) values of alcoholic extract of the selected plants were calculated and were (46.29±11.17) and (106.00±5.11) μg/mL, respectively. Quercetin was used as a positive control and its IC(50) value was (2.95±1.53) μg/mL.
  
  
  CONCLUSIONS
  Thus, it is concluded that alcoholic extracts of the selected plant exhibit significant inhibitory effects on AR in the rat lens in vitro.
authors.affiliation: Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Dinesh Kumar
authors.initials: DK
authors.lastname: Patel
authors.forename: Damiki
authors.initials: D
authors.lastname: Laloo
authors.forename: Krishnamurthy
authors.initials: K
authors.lastname: Sairam
authors.forename: Siva
authors.initials: S
authors.lastname: Hemalatha
mesh_terms: D032604:Alangiaceae / Q000737:chemistry*; D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D002386:Cataract / Q000188:drug therapy / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007887:Fabaceae / Q000737:chemistry*; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D000449:Aldehyde Reductase