title: Aquaporin-3 positively regulates matrix metalloproteinases via PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells.
abstract: BACKGROUND
  matrix metalloproteinases (MMPs) are produced by tumor cells, so they may be associated with tumor progression including invasion, migration, angiogenesis and metastasis. Aquaporin-3 (AQP3) also plays a critical role in gastric cancer cell migration and proliferation.
  
  
  METHODS
  In this study, AQP3 was silenced or over-expressed in SGC7901 cells.
  
  
  RESULTS
  We found a significant decrease in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 knockdown, and a significant increase in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 over-expression in SGC7901 cells. We also found that AQP3 silence led to a significant decrease of phosphorylation of ser473 in AKT in SGC7901 cells.
  
  
  CONCLUSIONS
  Our findings showed that AQP3 might positively regulate MMPs proteins expression through PI3K/AKT signal pathway in human gastric carcinoma SGC7901 cells.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Lizong
authors.initials: L
authors.lastname: Shen
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Zekuan
authors.initials: Z
authors.lastname: Xu
mesh_terms: D051400:Aquaporin 3 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C496027:AQP3 protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051400:Aquaporin 3; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C507086:MMP14 protein, human; D053511:Matrix Metalloproteinase 14