title: Improvement of the detection of neurodegenerative Alzheimer's disease through a specific surface chemistry applied onto the inner surface of the titration well.
abstract: The main objective of this paper was to illustrate the enhancement of the sensitivity of the ELISA titration of Tau proteins while reducing other non-specific adsorptions that could increase the optical densities and could lead to false positives. This goal was obtained thanks to the association of cold plasma and wet chemistries of the inner surface of the titration well. The PP surface was cold plasma-activated, then coated with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The support treated and coated with hexatrimethylammonium bromide improves the signal detection of proteins while reducing the background due to non-specific associations of biomolecules such as hyperphosphorylated Tau protein. However, coating with 3-butenylamine hydrochloride could also be suitable.
authors.affiliation: a LUNAM Universite, Laboratoire Polymeres, Colloides et Interfaces, UMR CNRS 6120 - Universite du Maine , Avenue O. Messiaen , 72085 , Le Mans , France.
authors.forename: C
authors.initials: C
authors.lastname: Mille
authors.forename: D
authors.initials: D
authors.lastname: Debarnot
authors.forename: W
authors.initials: W
authors.lastname: Zorzi
authors.forename: B
authors.initials: B
authors.lastname: El Moualij
authors.forename: A
authors.initials: A
authors.lastname: Coudreuse
authors.forename: G
authors.initials: G
authors.lastname: Legeay
authors.forename: I
authors.initials: I
authors.lastname: Quadrio
authors.forename: A
authors.initials: A
authors.lastname: Perret-Liaudet
authors.forename: F
authors.initials: F
authors.lastname: Poncin-Epaillard
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis* / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D066128:Gray Matter / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force; D010766:Phosphorylation; D013997:Time Factors; D017155:Wettability; D016875:tau Proteins / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D016875:tau Proteins