title: [Assessment of the distribution of nucleic acid intercalators in yeast cells by the pseudospectral image analysis].
abstract: The intracellular location of nucleic acid intercalators (NAI) in live (not fixed) Saccharomyces cerevisiae cells has been studied using fluorescence microscopy combined with computer pseudospectral image analysis. Three NAI: the anthracycline anticancer drug doxorubicin and the nucleic acid dyes ethidium bromide (E) and 4',6-diamidino-2-phenylindole (DAPI) were used. All three NAI were shown to be localized in nuclei and mitochondria. In contrast to DAPI, which interacted only with DNA, a large fraction of doxorubicin and ethidium bromide apparently bound to mitochondrial membranes. Upon combined application, a competition between these intercalators for binding sites in the nuclear and mitochondrial DNA occurred. It was concluded that this approach may be used in designing new DNA-targeted drugs and in preliminary studies of their interaction with eukaryotic cells.
authors.forename: E O
authors.initials: EO
authors.lastname: Puchkov
authors.forename: M
authors.initials: M
authors.lastname: McCarren
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D007211:Indoles / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D007211:Indoles; D007364:Intercalating Agents; C007293:DAPI; D004317:Doxorubicin; D004996:Ethidium