title: Comparison of the course of biomarker changes and kidney injury in a rat model of drug-induced acute kidney injury.
abstract: OBJECTIVE
  To aid in evaluating the performance of biomarkers, we measured kidney injury biomarkers in rat models of drug-induced acute kidney injury.
  
  
  RESULTS
  Rats were treated with site-specific nephrotoxins, puromycin, gentamicin, cisplatin, or 2-bromoethylamine. Fifteen biomarkers (β-2-microglobulin, calbindin, clusterin, cystatin-C, KIM-1, GST-α, GST-μ, NGAL, osteopontin, EGF, TIMP-1, VEGF, albumin, RPA-1, and urinary total protein) were examined in comparison with BUN, serum creatinine, and NAG. Some biomarkers, which were different depending in each nephrotoxin, showed ability to detect the prodromal stage of drug-induced kidney injury. Characteristic changing patterns of biomarkers were also found depending on the specific lesion site in the kidney.
  
  
  CONCLUSIONS
  These data suggested that establishment of a suitable biomarker panel would facilitate detection of site-specific kidney injury with high sensitivity.
authors.affiliation: Drug Safety Research Labs., Astellas Pharma Inc. , Kashima, Yodogawa-ku, Osaka , Japan. daisuke.sasaki@jp.astellas.com
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasaki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamada
authors.forename: Hitomi
authors.initials: H
authors.lastname: Umeno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurihara
authors.forename: Shunji
authors.initials: S
authors.lastname: Nakatsuji
authors.forename: Shiro
authors.initials: S
authors.lastname: Fujihira
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Tsubota
authors.forename: Mihoko
authors.initials: M
authors.lastname: Ono
authors.forename: Akira
authors.initials: A
authors.lastname: Moriguchi
authors.forename: Kouji
authors.initials: K
authors.lastname: Watanabe
authors.forename: Jiro
authors.initials: J
authors.lastname: Seki
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000175:diagnosis* / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000652:urine*; D001806:Blood Urea Nitrogen; D064026:Calbindins; D015815:Cell Adhesion Molecules / Q000652:urine; D002945:Cisplatin; D051152:Clusterin / Q000652:urine; D003404:Creatinine / Q000097:blood; D055316:Cystatin C / Q000652:urine; D005021:Ethylamines; D005839:Gentamicins; D007668:Kidney / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D008297:Male; D011691:Puromycin; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000652:urine; D012680:Sensitivity and Specificity; D001613:beta 2-Microglobulin / Q000652:urine
chemical_list: D015415:Biomarkers; D064026:Calbindins; D015815:Cell Adhesion Molecules; D002945:Cisplatin; D051152:Clusterin; D003404:Creatinine; D055316:Cystatin C; D005021:Ethylamines; D005839:Gentamicins; D011691:Puromycin; D064030:S100 Calcium Binding Protein G; D001613:beta 2-Microglobulin; C110936:Havcr1 protein, rat; C004504:2-bromoethylamine