title: Constitutive heat shock protein 70 interacts with α-enolase and protects cardiomyocytes against oxidative stress.
abstract: Constitutive heat shock protein 70 (Hsc70) is a molecular chaperone that has been shown to protect cardiomyocytes against oxidative stress. However, the molecular mechanism responsible for this protection remains uncertain. To understand the mechanism associated with the myocardial protective role of Hsc70, we have embarked upon a systematic search for Hsc70-interacting proteins. Using adenosine diphosphate (ADP) affinity chromatography and mass spectrometry, we have identified α-enolase, a rate-limiting enzyme in glycolysis, as a novel Hsc70-interacting protein in the myocardium of both sham and myocardial ischemia-reperfused Sprague-Dawley rat hearts. This interaction was confirmed by co-immunoprecipitation (IP) assays in the myocardial tissues and H9c2 cardiomyocytes and protein overlay assay (POA). It was further shown that Hsc70-overexpression alleviated the H(2)O(2)-induced decrease of α-enolase activity and cell damage, and Hsc70 deficiency aggravated the decrease of α-enolase activity and cell damage in H(2)O(2) treated H9c2 cells. Our research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with α-enolase.
authors.affiliation: Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Feng
authors.forename: Qinglan
authors.initials: Q
authors.lastname: Lv
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Shunlin
authors.initials: S
authors.lastname: Qu
authors.forename: Honglin
authors.initials: H
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000244:Adenosine Diphosphate / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D002846:Chromatography, Affinity; D015870:Gene Expression; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000378:metabolism*; D018384:Oxidative Stress; D010751:Phosphopyruvate Hydratase / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D050883:HSC70 Heat-Shock Proteins; C494373:Hspa8 protein, rat; D000244:Adenosine Diphosphate; D006861:Hydrogen Peroxide; D010751:Phosphopyruvate Hydratase