title: Development and characterization of a xylose-inducible gene expression system for Clostridium perfringens.
abstract: A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo kinase and xylose isomerase. pXCH allows tightly regulated expression of the chloramphenicol acetyltransferase reporter and the α-toxin genes in response to the inducer concentration. Thus, pXCH could constitute a new valuable genetic tool for study of C. perfringens.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. nariya@med.kagawa-u.ac.jp
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nariya
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyata
authors.forename: Tomomi
authors.initials: T
authors.lastname: Kuwahara
authors.forename: Akinobu
authors.initials: A
authors.lastname: Okabe
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D016360:Clostridioides difficile / Q000235:genetics; D003016:Clostridium perfringens / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D009875:Operator Regions, Genetic; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics; D014994:Xylose / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D002135:Calcium-Binding Proteins; D012097:Repressor Proteins; D014994:Xylose; D015500:Chloramphenicol O-Acetyltransferase; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase; D010738:Type C Phospholipases; C040649:alpha toxin, Clostridium perfringens; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase