title: The differentiation of humane adult mesenchimal stem cells of bone marrow (hMSC) into urothelial cells on bio-engineering support (scaffold): preliminary experience of tissue engineering.
abstract: OBJECTIVE
  Tissue Engineering can develop scaffolds of Poly-L-Lactic Acid (PLLA) for tissue regeneration. The purpose of the present job is to test the possibility to seed human adult mesenchymal stem cells on a scaffold supplemented with specific grow factors to differentiate them into urothelium.
  
  
  METHODS
  The Electrospinning technique was used to realize three scaffolds. The first one was seeded with urothelial cells, of a primary culture, and Keratinocyte serum free medium (KSFM); the second one was seeded with human mesenchymal stem cells (hMSC) and a minimum essential medium (aMEM); the third one was seeded with hMSC and conditioned medium.
  
  
  RESULTS
  Electron microscopy showed scaffolds with cellular vitality (>90%) and their cellular proliferation. Moreover, the differentiation of hMSC, seeded in conditioned medium, into urothelial cells was demonstrated through immunofluorescence assays.
  
  
  CONCLUSIONS
  Tissue Engineering can develop PLLA scaffolds thanks to the Electrospinning technique. The scaffold is a perfect environment for cellular culture and proliferation; a protocol for the differentiation of hMSC into urothelial cells is now available. Immunofluorescence assays can demonstrate the hMSC differentiation into urothelial cells.
authors.affiliation: Dipartimento di Urologia Università Campus Biomedico Roma, Italy. crisfalavolti@libero.it
authors.forename: Cristina
authors.initials: C
authors.lastname: Falavolti
authors.forename: Alberto
authors.initials: A
authors.lastname: Rainer
authors.forename: Matteo
authors.initials: M
authors.lastname: Centola
authors.forename: Marcella
authors.initials: M
authors.lastname: Trombetta
authors.forename: Franca
authors.initials: F
authors.lastname: Abbruzzese
authors.forename: Stefano
authors.initials: S
authors.lastname: Gidaro
authors.forename: Federico
authors.initials: F
authors.lastname: Sergi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Buscarini
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D019459:Urothelium / Q000166:cytology*