title: [High fat diet induces obesity and alters the expression  of MCHR1 and OB-Rb in the adipose tissue].
abstract: OBJECTIVE
  To investigate the effect of high-fat (HF) diet on the body weight and the mRNA expression of melanin concentrating hormone receptor 1 (MCHR1) and leptin receptor (OB-Rb) in the adipose tissue in rats, the two important and opposite factors in regulating the body weight.
  
  
  METHODS
  Post-weaning rats were divided into 3 groups: the NC group were fed a normal-chow diet (NC) (13% calories from fat), the HF group with a HF-diet (47% calories from fat) and the PHF group pair-fed a HF-diet (47% calories from fat). At the end of 8th week, the gained bodyweight, the plasma melanin concentrating hormone (MCH) and leptin, and the expression levels of MCHR1 and OB-Rb in the adipose tissue were measured.
  
  
  RESULTS
  Both the HF-diet and pair-fed HF-diet enhanced the body weight (P<0.01), plasma MCH (P<0.01) and leptin concentrations (P<0.05). In the adipose tissue, HF-diet resulted in significant increase in MCHR1 (PHF group,P<0.05) and decrease in OB-Rb mRNA levels (HF group,P<0.01; PHF group,P<0.05). No statistical difference was found between the HF group and the PHF group in terms of the aforementioned data (P>0.05).
  
  
  CONCLUSIONS
  Chronic intake of iso-caloric HF-diet and ad libitum HF-diet obviously results in increase in the body weight, serum leptin, and MCH concentration. Diet-induced obesity and related metabolic disorders are possibly correlated with up-regulated expression of MCHR1 and down-regulated expression of OB-Rb in the adipose tissue.
authors.affiliation: Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Jinrong
authors.initials: J
authors.lastname: Li
authors.forename: Jianqun
authors.initials: J
authors.lastname: Yan
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats / Q000008:administration & dosage; D007028:Hypothalamic Hormones / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D008543:Melanins / Q000097:blood; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D010907:Pituitary Hormones / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism*; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D007028:Hypothalamic Hormones; D020738:Leptin; C491208:MCHR1 protein, rat; D008543:Melanins; D010907:Pituitary Hormones; D012333:RNA, Messenger; D054411:Receptors, Leptin; D017481:Receptors, Somatostatin; C019463:melanin-concentrating hormone