title: [Filamentous fungi's cell-wall extraction at different stages of ontogenesis and exploration of their carbohydrate composition].
abstract: Methods of obtaining cell walls (CW) for specimens of mucoraceous molds and ascomycetic affined fungi are developed at the stage of mycelium and resting cells, or spores. CW purity was assessed by electron microscopy, specific staining methods, scourage control, presence of ribose and desoxyribose, and the comparison of chitin content in whole cells and CW of fungi (a new criteria). The authors discuss the significance of the proposed methods of obtaining pure fractions of CW and of the study of their carbohydrate content for the chemotaxonomy of filamentous fungi.
authors.forename: D A
authors.initials: DA
authors.lastname: Andrianova
authors.forename: Ia É
authors.initials: IaÉ
authors.lastname: Sergeeva
authors.forename: G A
authors.initials: GA
authors.lastname: Kochkina
authors.forename: L A
authors.initials: LA
authors.lastname: Galanina
authors.forename: A I
authors.initials: AI
authors.lastname: Usov
authors.forename: E P
authors.initials: EP
authors.lastname: Feofilova
mesh_terms: D005075:Biological Evolution; D002458:Cell Fractionation / Q000379:methods; D002473:Cell Wall / Q000737:chemistry* / Q000648:ultrastructure; D002686:Chitin / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D005658:Fungi / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009005:Monosaccharides / Q000032:analysis*; D009024:Morphogenesis / Q000502:physiology*; D025282:Mycelium / Q000737:chemistry* / Q000648:ultrastructure; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D013172:Spores, Fungal / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D009005:Monosaccharides; D010743:Phospholipids; D002686:Chitin