title: Dopamine 2 (DA-2) receptor assays using sympathetic nerve terminals.
abstract: In vitro experiments using isolated atria with and without superfusion can be used to quantify the dopamine-2 (DA-2) receptor stimulating activity of compounds. This receptor mechanism decreases the amount of norepinephrine released from sympathetic nerve terminals by neuronal impulses. DA-2 receptor agonists (stimulants) are very active and induce several physiological changes, such as bradycardia, hypotension, lower intraocular pressure, etc. In this unit, tissue preparation and dopamine receptor assays are described for isolated cat atria with field stimulation or with superfusion, and isolated guinea pig atria with field stimulation.
authors.affiliation: University of Iowa, Iowa City, Iowa, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Long
mesh_terms: D000320:Adrenergic Fibers / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002415:Cats; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D065127:Dopamine D2 Receptor Antagonists; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D009411:Nerve Endings / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods; D017448:Receptors, Dopamine D2 / Q000819:agonists / Q000502:physiology*; D013045:Species Specificity
chemical_list: D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D065127:Dopamine D2 Receptor Antagonists; D017448:Receptors, Dopamine D2