title: Identification of the bleomycin hydrolase gene as a methylated tumor suppressor gene in hepatocellular carcinoma using a novel triple-combination array method.
abstract: In the present study, we sought to identify novel suppressor genes of hepatocellular carcinoma (HCC) using our newly designed triple-combination array. Using this method, the bleomycin hydrolase gene (BLMH) was detected as a candidate suppressor gene. We found that 28 of 48 (58.3%) tumor tissues showed BLMH promoter hypermethylation, and its expression level was significantly reduced in tumor tissues (P=0.001). The present study suggests that our new method can detect novel genes of interest and that BLMH is a suppressor gene in HCC.
authors.affiliation: Department of Surgery II, Graduate School and Medicine, University of Nagoya, Showa-ku, Japan.
authors.forename: Yukiyasu
authors.initials: Y
authors.lastname: Okamura
authors.forename: Shuji
authors.initials: S
authors.lastname: Nomoto
authors.forename: Masamichi
authors.initials: M
authors.lastname: Hayashi
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Hishida
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Suguru
authors.initials: S
authors.lastname: Yamada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujii
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugimoto
authors.forename: Shin
authors.initials: S
authors.lastname: Takeda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kodera
authors.forename: Akimasa
authors.initials: A
authors.lastname: Nakao
mesh_terms: D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics*; D045744:Cell Line, Tumor; D003546:Cysteine Endopeptidases / Q000235:genetics*; D019175:DNA Methylation; D017931:DNA Primers; D016147:Genes, Tumor Suppressor; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D003546:Cysteine Endopeptidases; C031262:bleomycin hydrolase