title: Role of 5-HT(1A) and 5-HT(1B) receptors in the antidepressant-like effect of piperine in the forced swim test.
abstract: Our previous studies have showed that treating mice with piperine significantly decreased the immobility time of the animals in the forced swim test and tail suspension test, which was related to up-regulation of serotonin (5-HT) level in the brain. The purpose of this study is to explore the contribution of 5-HT receptors in the antidepressant-like effect of piperine. The results showed that pre-treating mice with methiothepin (a non-selective 5-HT receptor antagonist, 0.1mg/kg, intraperitoneally), 4-(2'-methoxy-phenyl)-1-[2'-(n-2″-pyridinyl)-p-iodobenzamino-]ethyl-piperazine (a selective 5-HT(1A) receptor antagonist, 1mg/kg, subcutaneously) or 1-(2-(1-pyrrolyl)-phenoxy)-3-isopropylamino-2-propanol (a 5-HT(1B) receptor antagonist, 2.5mg/kg, intraperitoneally) was found to abolish the anti-immobility effect of piperine (10mg/kg, intraperitoneally) in the forced swim test. On the other hand, a sub-effective dose of piperine (1mg/kg, intraperitoneally) produced a synergistic antidepressant-like effect with (+)-8-hydroxy-2-(di-n-propylamino)tetralin (a 5-HT(1A) receptor agonist, 1mg/kg, intraperitoneally) or anpirtoline (a 5-HT(1B) receptor agonist, 0.25mg/kg, intraperitoneally). Taken together, these results suggest that the antidepressant-like effect of piperine in the mouse forced swim test may be mediated, at least in part, by the activation of 5-HT(1A) and 5-HT(1B) receptors.
authors.affiliation: School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong; College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou, 310053 Zhejiang, China.
authors.forename: Qing-Qiu
authors.initials: QQ
authors.lastname: Mao
authors.affiliation: College of Pharmacy, Zhejiang Chinese Medicine University, Hangzhou, 310053 Zhejiang, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong. Electronic address: paulip@cuhk.edu.hk.
authors.forename: Siu-Po
authors.initials: SP
authors.lastname: Ip
authors.affiliation: School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Xian
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, Chicago, IL 60612, USA.
authors.forename: Chun-Tao
authors.initials: CT
authors.lastname: Che
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000494:pharmacology; D000470:Alkaloids / Q000494:pharmacology*; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D000928:Antidepressive Agents; D052117:Benzodioxoles / Q000494:pharmacology*; D003627:Data Interpretation, Statistical; D008297:Male; D008719:Methiothepin / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009043:Motor Activity / Q000187:drug effects; D010879:Piperazines / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology*; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D011412:Propanolamines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D044282:Receptor, Serotonin, 5-HT1A / Q000187:drug effects*; D044302:Receptor, Serotonin, 5-HT1B / Q000187:drug effects*; D058825:Serotonin 5-HT1 Receptor Agonists / Q000494:pharmacology; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000494:pharmacology; D013550:Swimming / Q000523:psychology*
chemical_list: D000470:Alkaloids; D000631:Aminopyridines; D000928:Antidepressive Agents; D052117:Benzodioxoles; D010879:Piperazines; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D011412:Propanolamines; D011725:Pyridines; D044302:Receptor, Serotonin, 5-HT1B; D058825:Serotonin 5-HT1 Receptor Agonists; D058829:Serotonin 5-HT1 Receptor Antagonists; D044282:Receptor, Serotonin, 5-HT1A; C087205:4-(2'-methoxyphenyl)-1-(2'-(N-(2''-pyridinyl)-4-iodobenzamido)ethyl)piperazine; C075631:anpirtoline; D008719:Methiothepin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; C058392:isamoltane; C008922:piperine