title: Identification and characterization of feline UBE1L gene.
abstract: Interferon-stimulated gene 15 (ISG15) is one of the type I interferon-inducible proteins. Addition of ISG15 known as ISGylation is an ubiquitin-like posttranslational modification. Coexpression of ISG15 and ubiquitin-activating enzyme E1-like protein (UBE1L) is required to induce ISGylation. Previously, we identified feline ISG15 gene and found that the capsid protein of feline immunodeficiency virus was ISGylated in vitro by treatment with feline interferon-ω. In this study, we cloned feline UBE1L (FeUBE1L) gene to further study the mechanism of the antiviral activities induced by ISGylation. Sequencing analysis revealed that active sites of FeUBE1L were highly conserved. These data suggest that FeUBE1L has an enzymatic activity. Further, expression of FeUBE1L was induced in feline cell lines by treatment with feline interferon-ω and ovine interferon-τ.
authors.affiliation: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606–8501, Japan.
authors.forename: Sayumi
authors.initials: S
authors.lastname: Shimode
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Sato
authors.forename: Taishi
authors.initials: T
authors.lastname: Tanabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D016181:Feline Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D044764:Ubiquitin-Activating Enzymes / Q000235:genetics* / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
chemical_list: D014452:Ubiquitins; D004247:DNA; D044764:Ubiquitin-Activating Enzymes