title: Interaction of CCND1 genotype and smoking habit in Taiwan lung cancer patients.
abstract: OBJECTIVE
  Cyclin D1 (CCND1) is critical in the transition of the cell cycle from G1 to S phase and unbalanced cell cycle regulation is a hallmark of carcinogenesis. The study aimed at investigating the association of CCND1 genotypes with lung cancer risk in Taiwan and examining the interaction between CCND1 genotype and smoking habit.
  
  
  METHODS
  CCND1 A870G (rs9344) and C1722G (rs678653) genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of DNA from the blood of 358 lung cancer patients and 716 cancer-free healthy controls.
  
  
  RESULTS
  The results showed that there were significant differences between lung cancer and control groups in the distribution of the genotypes (p=0.0003) and allelic frequency (p=0.0007) in the CCND1 rs9344 genotype. Individuals who carried AG or GG genotype had 0.59- and 0.52-fold risk, respectively, of developing lung cancer compared to those who carried the AA genotype (95% CI=0.44-0.78 and 0.35-0.79, respectively). There was also an obvious interaction of CCND1 rs9344 genotype with personal smoking habit on lung cancer risk (p=0.0009).
  
  
  CONCLUSIONS
  These findings support the conclusion that cell cycle regulation may play a role in lung cancer development and that CCND1 rs9344 polymorphism together with smoking habit maybe a useful biomarker for lung cancer prediction.
authors.affiliation: Department of Pulmonary and Critical Care Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.
authors.forename: Te-Chun
authors.initials: TC
authors.lastname: Hsia
authors.forename: Chin-Jung
authors.initials: CJ
authors.lastname: Liu
authors.forename: Chih-Hsueh
authors.initials: CH
authors.lastname: Lin
authors.forename: Wen-Shin
authors.initials: WS
authors.lastname: Chang
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Chu
authors.forename: Liang-Wen
authors.initials: LW
authors.lastname: Hang
authors.forename: Hong-Zin
authors.initials: HZ
authors.lastname: Lee
authors.forename: Woei-Chung
authors.initials: WC
authors.lastname: Lo
authors.forename: Da-Tian
authors.initials: DT
authors.lastname: Bau
mesh_terms: D016022:Case-Control Studies; D019938:Cyclin D1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012907:Smoking / Q000235:genetics*; D013624:Taiwan
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1