title: Longitudinal field study on bovine Babesia spp. and Anaplasma phagocytophilum infections during a grazing season in Belgium.
abstract: Anaplasmosis and babesiosis are major tick-borne diseases with a high economic impact but are also a public health concern. Blood samples collected in the spring, summer, and autumn of 2010 from 65 cows in seven different farms in Belgium were monitored with an indirect immunofluorescence antibody test to assess seroprevalence against these pathogens. Seroprevalences to Babesia spp. were measured as 10.7%, 20%, and 12.3% in spring, summer, and autumn, respectively, whereas seroprevalences to Anaplasma phagocytophilum were 30.8%, 77%, and 56.9%, respectively. A total of 805 Ixodes ricinus ticks were collected at the same time from both cattle (feeding ticks) and grazed pastures (questing ticks). The infection level of ticks, assessed by PCR assay, for Babesia spp. DNA was 14.6% and 7.9% in feeding and questing ticks, respectively, whereas 21.7% and 3% of feeding and questing ticks were found be positive for A. phagocytophilum cDNA. Fifty-five PCR-positive samples were identified by sequencing as Babesia sp. EU1, of which five from feeding ticks were positive for both A. phagocytophilum and Babesia sp. EU1. The high density of wild cervids in the study area could explain these observations, as deer are considered to be the main hosts for adults of I. ricinus. However, the absence of Babesia divergens both in feeding and questing ticks is surprising, as the study area is known to be endemic for cattle babesiosis. Increasing cervid populations and comorbidity could play an import role in the epidemiology of these tick-borne diseases.
authors.affiliation: Laboratory of Parasitology and Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium. l.lempereur.1@research.gla.ac.uk
authors.forename: Laetitia
authors.initials: L
authors.lastname: Lempereur
authors.forename: Maude
authors.initials: M
authors.lastname: Lebrun
authors.forename: Pascale
authors.initials: P
authors.lastname: Cuvelier
authors.forename: Géraldine
authors.initials: G
authors.lastname: Sépult
authors.forename: Yannick
authors.initials: Y
authors.lastname: Caron
authors.forename: Claude
authors.initials: C
authors.lastname: Saegerman
authors.forename: Brian
authors.initials: B
authors.lastname: Shiels
authors.forename: Bertrand
authors.initials: B
authors.lastname: Losson
mesh_terms: D041081:Anaplasma phagocytophilum / Q000302:isolation & purification / Q000472:pathogenicity*; D000818:Animals; D001403:Babesia / Q000302:isolation & purification / Q000472:pathogenicity*; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D001530:Belgium / Q000453:epidemiology; D002417:Cattle / Q000469:parasitology*; D003670:Deer / Q000469:parasitology; D005260:Female; D018884:Ixodes / Q000254:growth & development / Q000472:pathogenicity; D008137:Longitudinal Studies; D012621:Seasons; D016036:Seroepidemiologic Studies; D017282:Tick-Borne Diseases / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D013987:Ticks / Q000254:growth & development / Q000472:pathogenicity