title: Genome wide expression analysis of the effect of the Chinese patent medicine Zilongjin tablet on four human lung carcinoma cell lines.
abstract: Zilongjin (ZLJ) tablet, which is a traditional Chinese medicine, has been approved as a new anti-tumor drug by the State Food and Drug Administration of China; however, its anti-cancer mechanisms remain elusive. The goal of this study was to investigate the underlying anti-cancer activities of ZLJ tablet in vitro. In this study, four lung cancer cell lines, A549, H446, H460 and H520, were treated with 2.2 mg/mL of ZLJ solution for 24 h at 37 °C under 5% CO(2) . RNA was isolated and a microarray experiment using the Affymetrix Human Genome U133 plus 2.0 Array was employed to differentiate the expression patterns of cancer-related genes after drug treatment. Of 483 genes in 63 functional categories and 25 different pathways that showed at least a 2-fold change of expression level in the four cancer cell lines, 170 genes were upregulated, and 313 genes were downregulated. Eleven of the 483 genes were cancer-related and belong to the three known pathways: apoptosis, cell cycle regulation and mitogen-activated protein kinase (MAPK) cascade. The microarray data were validated by real-time RT-PCR. The results of this investigation suggest possible anti-cancer mechanisms of the ZLJ tablet, and lay a foundation to further analyse its therapeutic roles.
authors.affiliation: Tianjin Zhongxin Pharmaceutical Group Co., Ltd, Nankai District, Tianjin, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Songjin
authors.initials: S
authors.lastname: Xiong
authors.forename: Shaoping
authors.initials: S
authors.lastname: Wen
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Boyang
authors.initials: B
authors.lastname: Cao
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D052138:Genes, Neoplasm / Q000187:drug effects*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000235:genetics*; D019684:Magnoliopsida / Q000737:chemistry*; D046228:Microarray Analysis / Q000379:methods; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics; D008517:Phytotherapy; D012333:RNA, Messenger / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C558114:zilongjin; D020928:Mitogen-Activated Protein Kinases