title: Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture.
abstract: Effects of different pulse fed-batch methods on production of the anti-cancer compound 1403C by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403) in a 5-L bioreactor were investigated. Since high glucose concentrations improved mycelial growth but inhibited 1403C production, the cultures were pulse fed with glucose solutions to keep the residual glucose lower than 4 g/L but higher than 0.5 g/L during rapid growth phase (0-50 h). In this way, a maximum dry biomass, 1403C production and yield coefficient (Y1403C/X) of up to 4.5 g/L, 2.64 g/L and 0.59 g/g dry cell weight, respectively were achieved. These values are 22.7%, 98.0% and 61.4%, respectively higher than those obtained with batch cultures. This strategy is valuable for fermentation scale-up of Halorosellinia sp. (No. 1403) for 1403C production, and might also be applicable to other marine fungi cultures.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.forename: Menghao
authors.initials: M
authors.lastname: Cai
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.forename: Yuanxing
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangshan
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000378:metabolism*; D001203:Ascomycota / Q000187:drug effects* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques / Q000379:methods*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D005947:Glucose / Q000494:pharmacology*; D007700:Kinetics; D012623:Seawater / Q000382:microbiology*
chemical_list: D000880:Anthraquinones; D000970:Antineoplastic Agents; C569938:SZ 685C; D005947:Glucose