title: Foot-and-mouth disease virus (FMDV) leader proteinase negatively regulates the porcine interferon-λ1 pathway.
abstract: Foot-and-mouth disease is a highly contagious viral disease caused by foot-and-mouth disease virus (FMDV) of wild and domestic cloven-hoofed animals, and causes an economically important disease in the swine industry. In this study, we found that the replication of FMDV in IBRS-2 cells could be significantly inhibited after treatment with the purified recombinant porcine interferon lambda 1 (IFN-λ1), a newly identified type III interferon. However, FMDV could not activate the IFN-λ1 promoter and IFN-λ1 mRNA expression in infected IBRS-2 cells, suggesting that FMDV has evolved mechanisms to interrupt the antiviral function of IFN-λ1. The cause of this inhibition was determined by screening all structural and non-structural proteins of FMDV, and the leader proteinase (L(pro)) was found to exhibit the highest potential to inhibit poly(I:C)-induced IFN-λ1 promoter activity. Further study revealed that the catalytic activity and a SAP (SAF-A/B, Acinus, and PIAS) domain of L(pro) were required for suppressing poly(I:C)-induced IFN-λ1 production. These data suggest that FMDV replication could be inhibited by porcine IFN-λ1, but that the virus has evolved specific mechanisms to inhibit this action.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Dang
authors.initials: D
authors.lastname: Wang
authors.forename: Liurong
authors.initials: L
authors.lastname: Fang
authors.forename: Lizhi
authors.initials: L
authors.lastname: Liu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhong
authors.forename: Quangang
authors.initials: Q
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Luo
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Liu
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Zhang
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000818:Animals; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology* / Q000276:immunology; D007372:Interferons / Q000276:immunology*; D010447:Peptide Hydrolases / Q000276:immunology* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
chemical_list: D011994:Recombinant Proteins; D007372:Interferons; D010447:Peptide Hydrolases