title: Remote exosites of the catalytic domain of matrix metalloproteinase-12 enhance elastin degradation.
abstract: How does matrix metalloproteinase-12 (MMP-12 or metalloelastase) degrade elastin with high specific activity? Nuclear magnetic resonance suggested soluble elastin covers surfaces of MMP-12 far from its active site. Two of these surfaces have been found, by mutagenesis guided by the BINDSIght approach, to affect degradation and affinity for elastin substrates but not a small peptide substrate. Main exosite 1 has been extended to Asp124 that binds calcium. Novel exosite 2 comprises residues from the II-III loop and β-strand I near the back of the catalytic domain. The high degree of exposure of these distal exosites may make them accessible to elastin made more flexible by partial hydrolysis. Importantly, the combination of one lesion each at exosites 1 and 2 and the active site decreased the catalytic competence toward soluble elastin by 13-18-fold to the level of MMP-3, homologue and poor elastase. Double-mutant cycle analysis of conservative mutations of Met156 (exosite 2) and either Asp124 (exosite 1) or Ile180 (active site) showed they had additive effects. Compared to polar substitutions observed in other MMPs, Met156 enhanced affinity and Ile180 the k(cat) for soluble elastin. Both residues detracted from the higher folding stability with polar mutations. This resembles the trend in enzymes of an inverse relationship between folding stability and activity. Restoring Asp124 from combination mutants enhanced the k(cat) for soluble elastin. In elastin degradation, exosites 1 and 2 contributed in a manner independent of each other and Ile180 at the active site, but with partial coupling to Ala182 near the active site. The concept of weak, separated interactions coalescing somewhat independently can be extended to this proteolytic digestion of a protein from fibrils.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia, Missouri 65211, United States.
authors.forename: Yan G
authors.initials: YG
authors.lastname: Fulcher
authors.forename: Steven R
authors.initials: SR
authors.lastname: Van Doren
mesh_terms: D001224:Aspartic Acid / Q000737:chemistry / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D020134:Catalytic Domain; D015536:Down-Regulation / Q000235:genetics; D004549:Elastin / Q000378:metabolism*; D004795:Enzyme Stability / Q000235:genetics; D006801:Humans; D006868:Hydrolysis; D053508:Matrix Metalloproteinase 12 / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D017510:Protein Folding; D012995:Solubility; D013379:Substrate Specificity / Q000235:genetics
chemical_list: D002135:Calcium-Binding Proteins; D001224:Aspartic Acid; D004549:Elastin; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12