title: The tropism of neurally differentiated bone marrow stromal cells towards C6 glioma.
abstract: Recent studies have indicated that bone marrow stromal cells (BMSCs) have significant tropism towards glioma which makes them play an important role in carrying genes/drugs to inhibit the growth of glioma as cell vehicles. But BMSCs may differentiate into neural cells under entocranial environment and few researches support the idea that neurally differentiated bone marrow stromal cells (N-D-BMSCs) still hold the capacity of migrating to the tumor sites. The aim of our study was to investigate the tropism of N-D-BMSCs towards C6 glioma. In vitro migration assay was employed by transwell co-culture system and Student's t-test analysis indicated that N-D-BMSCs had the significant tropism towards C6 glioma-conditioned medium (GCM) (P<0.01). Furthermore, the vascular endothelial growth factor (VEGF) bioactivity of the C6 GCM was neutralized by the anti-rat VEGF antibody and our data suggested that the VEGF from C6 GCM hold chemoattraction for N-D-BMSCs and some other cytokines from the C6 GCM may be responsible for the chemoattraction for N-D-BMSCs. In vivo migration assay was carried out with cells transplantation and one way ANOVA analysis indicated that the tropism of N-D-BMSCs towards C6 glioma sites presented time variation (P-value=2.9E-20). Moreover, multiple comparisons for the time variables with the Student's t-test and the results suggested that the migration capacity of N-D-BMSCs towards C6 glioma sites reach the peak on the 7th day after transplantation. These results demonstrate that N-D-BMSCs as well as BMSCs have significant tropism towards C6 glioma.
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Qianfa
authors.initials: Q
authors.lastname: Long
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China. Electronic address: liuwp@fmmu.edu.cn.
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Xicai
authors.initials: X
authors.lastname: Yi
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Yuanyang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Neurology, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.affiliation: Xijing Institute of Clinical Neuroscience, Fourth Military Medical University, 17 Chang-le West Road, Xi'an 710032, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fei
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000502:physiology*; D001932:Brain Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D017690:Cell Transplantation; D018920:Coculture Techniques; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000502:physiology*; D018523:Tropism / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000502:physiology
chemical_list: D019138:Antibodies, Blocking; D042461:Vascular Endothelial Growth Factor A