title: Monitoring mRNA in living cells in a 3D in vitro model using TAT-peptide linked molecular beacons.
abstract: There is a growing need for the development of in vitro 3D cell culture models for assessing newer therapeutics for clinical applications and mechanisms of human pathology. Molecular beacons have been successfully delivered in two-dimensional (2D) systems to monitor, detect, and localize specific mRNA expression in living cells at the single cell level. However, to date the use of molecular beacons in three-dimensional (3D) systems has not been reported. To translate this technology into specific clinical targeted applications, it is critical to develop and demonstrate efficacy in a 3D system. For the first time the use of TAT-peptide conjugated molecular beacons to monitor mRNA in a 3D in vitro system has been reported.
authors.affiliation: Network of Excellence for Functional Biomaterials (NFB), National University of Ireland, Galway, Ireland.
authors.forename: Jennifer Claire
authors.initials: JC
authors.lastname: Alexander
authors.forename: Abhay
authors.initials: A
authors.lastname: Pandit
authors.forename: Gang
authors.initials: G
authors.lastname: Bao
authors.forename: David
authors.initials: D
authors.lastname: Connolly
authors.forename: Yury
authors.initials: Y
authors.lastname: Rochev
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D018929:Cell Culture Techniques; D002470:Cell Survival; D024042:Collagen Type I / Q000378:metabolism; D005786:Gene Expression Regulation; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054457:Tissue Scaffolds; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D012333:RNA, Messenger; D054322:tat Gene Products, Human Immunodeficiency Virus; C497137:tat peptide (47-57), Human immunodeficiency virus 1; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)