title: [Translation termination factor of eRFI of the ciliate Blepharisma japonicum recognizes all three stop codons].
abstract: We have determined the type of stop codon specificity of Blepharisma japonicum translation termination factor eRF1 in an in vitro reconstituted eukaryotic translation system and in in vivo assay (the dual reporter system). We have shown that B. japonicum eRF1 retained specificity towards all three stop codons although efficiency of peptydyl-tRNA hydrolysis in the presence of UGA is reduced in an in vitro assay. We suggest that since the heterotrich B. japonicum represents the earliest diverged lineage on phylogenetic tree of ciliates, B. japonicum has the universal genetic code as ancestor group for all ciliates.
authors.forename: B D
authors.initials: BD
authors.lastname: Eliseev
authors.forename: E Z
authors.initials: EZ
authors.lastname: Alkalaeva
authors.forename: P N
authors.initials: PN
authors.lastname: Kriuchkova
authors.forename: S A
authors.initials: SA
authors.lastname: Lekomtsev
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ai-hua
authors.initials: AH
authors.lastname: Liang
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Frolova
mesh_terms: D000595:Amino Acid Sequence; D016798:Ciliophora / Q000235:genetics*; D018388:Codon, Terminator / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010443:Peptide Chain Termination, Translational / Q000235:genetics*; D010454:Peptide Termination Factors / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D018388:Codon, Terminator; D010454:Peptide Termination Factors; D012343:RNA, Transfer