title: [Expression of aldolase of Toxoplasma gondii and preparation of the polyclonal antibodies against this protein].
abstract: OBJECTIVE
  To produce the aldolase protein and its polyclonal antibody.
  
  
  METHODS
  Aldolase gene was obtained from cDNA library by PCR amplification and subcloned to vector pET30a. The recombinant protein aldolase-His(6); was expressed in E.coli upon IPTG induction and then purified with affinity chromatography. The purified protein mixed with adjuvant was used to immunize SD rats to produce the polyclonal antibodies.
  
  
  RESULTS
  The recombinant plasmid aldolase/pET30a was constructed successfully and expressed as a fusion protein aldolase-His(6);;  Polyclonal antibody against aldolase-His(6); was obtained from rat,  and antibody titer was 1:4 000.
  
  
  CONCLUSIONS
  The purified protein aldolase-His(6); and its polyclonal antibodies were obtained,  which may provide the foundation for the further studies on the function of aldolase.
authors.affiliation: College of Pharmacy, Xinxiang Medical University, Xinxiang, China.
authors.forename: Zhi-kui
authors.initials: ZK
authors.lastname: Yin
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Deng
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D014122:Toxoplasma / Q000235:genetics / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D011993:Recombinant Fusion Proteins; D005634:Fructose-Bisphosphate Aldolase