title: [The effect of diet induced obesity on testicular tissue and serum oxidative stress parameters].
abstract: OBJECTIVE
  The aim of this study was to evaluate the effects of diet induced obesity on semen parameters and serum antioxidant enzyme levels.
  
  
  METHODS
  Six-week-old male rats were randomized into three groups are as follows: group 1 (n=10) received a control diet, group 2 (n=9) received a high-fat diet and group 3 (n=11) received high-fat diet plus anastrozole. At the completion of a 10-week period, testicular tissues were obtained and spermatogenesis was evaluated with Johnsen Score System. The normal Johnsen Score was accepted as >9.39. In addition, serum antioxidant enzyme levels, triglyceride, cholesterol, testosterone, luteinizing hormone (LH), follicle stimilating hormone (FSH) and estradiol levels were measured in serum.
  
  
  RESULTS
  Body weight were significantly increased in mice fed with a high-fat diet compared to normal diet (P<.05). The mean triglyceride levels was 64.00±20.48 mg/dl, 98.89±27.80 mg/dl and 95.27±15.02 mg/dl in group 1, group 2 and group 3, respectively (P<.05). Male rats fed with a high-fat diet had significantly lower levels of testosterone compared with the control diet male rats (P=.005). Testicular pathology revealed that Johnsen Score System were 9.60±0.15, 8.72±1.81 and 9.29 in group 1, group 2 and group 3, respectively (P=.169). In addition serum nitric oxide (NO) levels were higher in group 2 and group 3 compared to group 1 (P<.05).
  
  
  CONCLUSIONS
  As a result it may be concluded that obesity may induce oxidative stress and decrease testosterone levels. These changes may alter testicular functions and consequently it may be speculated that obesity can be important causative factor in the etiology of the male infertility.
authors.affiliation: Departamento de Urología, Universidad Gaziosmanpasa, Tokat, Turquía. fikreterdemir@mynet.com
authors.forename: F
authors.initials: F
authors.lastname: Erdemir
authors.forename: D
authors.initials: D
authors.lastname: Atilgan
authors.forename: F
authors.initials: F
authors.lastname: Markoc
authors.forename: O
authors.initials: O
authors.lastname: Boztepe
authors.forename: B
authors.initials: B
authors.lastname: Suha-Parlaktas
authors.forename: S
authors.initials: S
authors.lastname: Sahin
mesh_terms: D000077384:Anastrozole; D000818:Animals; D047072:Aromatase Inhibitors / Q000627:therapeutic use; D015415:Biomarkers; D001835:Body Weight; D002374:Catalase / Q000097:blood; D059305:Diet, High-Fat / Q000009:adverse effects*; D005978:Glutathione / Q000097:blood; D012739:Gonadal Steroid Hormones / Q000097:blood; D007248:Infertility, Male / Q000097:blood / Q000209:etiology; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D009570:Nitriles / Q000627:therapeutic use; D009765:Obesity / Q000209:etiology / Q000378:metabolism*; D018384:Oxidative Stress; D010908:Pituitary Hormones, Anterior / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013091:Spermatogenesis; D013482:Superoxide Dismutase / Q000097:blood; D000072105:Superoxide Dismutase-1; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D014230:Triazoles / Q000627:therapeutic use
chemical_list: D047072:Aromatase Inhibitors; D015415:Biomarkers; D012739:Gonadal Steroid Hormones; D008055:Lipids; D009570:Nitriles; D010908:Pituitary Hormones, Anterior; D014230:Triazoles; D000077384:Anastrozole; D009569:Nitric Oxide; D002374:Catalase; C000606291:Sod1 protein, mouse; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D005978:Glutathione