title: Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization.
abstract: Swertia tetraptera Maxim. is an important source of secoiridoid glucosides. To produce these pharmacologically valuable compounds heterologously in somatic hybrid cell lines, S. tetraptera protoplasts were irradiated with various doses of UV light and fused with protoplasts from a long-term cell line of Bupleurum scorzonerifolium Willd. This recipient was chosen as the cell line is cytogenetically stable and fast growing; furthermore, protoplasts isolated from the cell line are readily regenerable. From a set of 86 putative hybrid calli, only two were able to regenerate viable green plants. The hybridity of the 19 of the 86 selections was revealed by a combined isozyme and RAPD analysis, supported by a karyotypic study based on genomic in situ hybridization (GISH). Clone I-3 contained 0.014% swertiamarin while the regenerants had 0.069% swertiamarin and 0.409% gentiopicroside while the III-4 plants contained only 0.015% gentiopicroside.
authors.affiliation: Anhui Medical University, Hefei, 230061, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Cai
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
authors.forename: Fengning
authors.initials: F
authors.lastname: Xiang
mesh_terms: D029972:Bupleurum / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D032461:Chromosomes, Plant / Q000378:metabolism; D046508:Culture Techniques; D004950:Esterases / Q000235:genetics; D051456:Gene Flow; D054628:Genome, Chloroplast; D005838:Genotype; D006824:Hybridization, Genetic; D057888:Iridoid Glucosides / Q000302:isolation & purification / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D059785:Karyotype; D018895:Microsatellite Repeats; D010940:Plant Proteins / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism; D011753:Pyrones / Q000302:isolation & purification / Q000378:metabolism; D029967:Swertia / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
chemical_list: D057888:Iridoid Glucosides; D007527:Isoenzymes; D010940:Plant Proteins; D011753:Pyrones; C012997:gentiopicroside; C013270:swertiamarin; D004950:Esterases