title: Sleep loss alters synaptic and intrinsic neuronal properties in mouse prefrontal cortex.
abstract: Despite sleep-loss-induced cognitive deficits, little is known about the cellular adaptations that occur with sleep loss. We used brain slices obtained from mice that were sleep deprived for 8h to examine the electrophysiological effects of sleep deprivation (SD). We employed a modified pedestal (flowerpot) over water method for SD that eliminated rapid eye movement sleep and greatly reduced non-rapid eye movement sleep. In layer V/VI pyramidal cells of the medial prefrontal cortex, miniature excitatory post synaptic current amplitude was slightly reduced, miniature inhibitory post synaptic currents were unaffected, and intrinsic membrane excitability was increased after SD.
authors.affiliation: Program in Neuroscience, Washington State University, Pullman, WA 99164-6520, USA. bwinters@vetmed.wsu.edu
authors.forename: Bradley D
authors.initials: BD
authors.lastname: Winters
authors.forename: Yanhua H
authors.initials: YH
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.forename: James M
authors.initials: JM
authors.lastname: Krueger
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D004558:Electric Stimulation; D004569:Electroencephalography; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D005133:Eye Movements; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018408:Patch-Clamp Techniques; D017397:Prefrontal Cortex / Q000473:pathology*; D017966:Pyramidal Cells / Q000187:drug effects / Q000503:physiopathology*; D012892:Sleep Deprivation / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors