title: N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars.
abstract: The main aim of our study was to determine the physiological function of NagA enzyme in the Listeria monocytogenes cell. The primary structure of the murein of L. monocytogenes is very similar to that of Escherichia coli, the main differences being amidation of diaminopimelic acid and partial de-N-acetylation of glucosamine residues. NagA is needed for the deacetylation of N-acetyl-glucosamine-6 phosphate to glucosamine-6 phosphate and acetate. Analysis of the L. monocytogenes genome reveals the presence of two proteins with NagA domain, Lmo0956 and Lmo2108, which are cytoplasmic putative proteins. We introduced independent mutations into the structural genes for the two proteins. In-depth characterization of one of these mutants, MN1, deficient in protein Lmo0956 revealed strikingly altered cell morphology, strongly reduced cell wall murein content and decreased sensitivity to cell wall hydrolase, mutanolysin and peptide antibiotic, colistin. The gene products of operon 150, consisting of three genes: lmo0956, lmo0957, and lmo0958, are necessary for the cytosolic steps of the amino-sugar-recycling pathway. The cytoplasmic de-N-acetylase Lmo0956 of L. monocytogenes is required for cell wall peptidoglycan and teichoic acid biosynthesis and is also essential for bacterial cell growth, cell division, and sensitivity to cell wall hydrolases and peptide antibiotics.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland. magdapop@biol.uw.edu.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Popowska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Osińska
authors.forename: Magdalena
authors.initials: M
authors.lastname: Rzeczkowska
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D000581:Amidohydrolases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000201:enzymology; D003091:Colistin / Q000494:pharmacology; D010450:Endopeptidases / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D008089:Listeria monocytogenes / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D016254:Mutagenesis, Insertional; D009876:Operon; D010457:Peptidoglycan / Q000096:biosynthesis; D017434:Protein Structure, Tertiary; D013682:Teichoic Acids / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D010457:Peptidoglycan; D013682:Teichoic Acids; C001293:glucosamine 6-phosphate; D019298:Glucose-6-Phosphate; D010450:Endopeptidases; C021816:mutanolysin; D000581:Amidohydrolases; C018717:N-acetylglucosamine-6-phosphate deacetylase; D005944:Glucosamine; D000117:Acetylglucosamine; D003091:Colistin