title: Assessing mitochondrial redox status by flow cytometric methods: vascular response to fluid shear stress.
abstract: Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular diseases. Vascular oxidative stress is intimately linked with pro-inflammatory states and atherosclerosis. Oxidized low-density lipoprotein (OxLDL) modulates intracellular redox status and induces apoptosis in endothelial cells. Hemodynamic, specifically, fluid shear stress imparts both biomechanical and metabolic effects on vasculature. Mitochondria are an important source of superoxide production contributing to vascular oxidative stress with relevance to cardiovascular diseases. We hereby present biophysical and biochemical approaches, including fluorescence-activated cell sorting, to assess the dynamics of vascular redox status.
authors.affiliation: Cardiovascular Engineering Research, Department of Biomedical Engineering and Cardiology, School of Medicine and School of Engineering, University of Southern California, Los Angeles, California.
authors.forename: Rongsong
authors.initials: R
authors.lastname: Li
authors.affiliation: Cardiovascular Engineering Research, Department of Biomedical Engineering and Cardiology, School of Medicine and School of Engineering, University of Southern California, Los Angeles, California.
authors.forename: Nelson
authors.initials: N
authors.lastname: Jen
authors.affiliation: Cardiovascular Engineering Research, Department of Biomedical Engineering and Cardiology, School of Medicine and School of Engineering, University of Southern California, Los Angeles, California.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.affiliation: Cardiovascular Engineering Research, Department of Biomedical Engineering and Cardiology, School of Medicine and School of Engineering, University of Southern California, Los Angeles, California.
authors.forename: Tzung K
authors.initials: TK
authors.lastname: Hsiai
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology; D002417:Cattle; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry / Q000379:methods*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D033081:Shear Strength
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein