title: [Rapid detection of chromosome number aberration in spontaneously aborted fetuses using fluorescence in situ hybridization].
abstract: OBJECTIVE
  To assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of common chromosome number aberration in spontaneously aborted fetuses.
  
  
  METHODS
  A total of 100 spontaneously aborted fetuses were analyzed by G-banding and by FISH to test chromosome number aberration mainly for chromosomes 13, 18, 21, X and Y, and the results of FISH test was assessed according to those by G-banding test.
  
  
  RESULTS
  FISH results were well consistent with those by G-banding test. FISH test identified trisomy in 32 samples and polyploidy in 7 samples. Two samples with cell culture failure were found to have trisomy 16 by FISH. Discrepancies in the results between the two tests occurred in 3 samples, but the results of FISH were verified by other methods. Kappa test between FISH technology and G-banding showed a good consistency between FISH and karyotyping (P<0.05).
  
  
  CONCLUSIONS
  FISH is an effective and rapid method for detecting chromosome number aberration in spontaneously aborted fetuses, and the combination of FISH and karyotyping provides more reliable diagnostic evidence.
authors.affiliation: Department of Obstetrics and Gynecology, Southern Medical University, Guangzhou 510515, China.
authors.forename: Lan-lin
authors.initials: LL
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Xiong
authors.forename: Si-ping
authors.initials: SP
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Gao
authors.forename: Rui-feng
authors.initials: RF
authors.lastname: Wu
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Yu
mesh_terms: D037881:Aborted Fetus; D002869:Chromosome Aberrations; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D011247:Pregnancy