title: Illumination of Arabidopsis roots induces immediate burst of ROS production.
abstract: Arabidopsis roots are routinely exposed to light both during their cultivation within transparent Petri dishes and during their confocal microscopy analysis. Here we report that illumination of roots which naturally grow in darkness, even for a few seconds, induces an immediate and strong burst of reactive oxygen species (ROS). Plant scientists studying roots should pay great attention to the environment of living roots, and keep them in darkness as long as possible. Results obtained using illuminated roots during in vivo microscopic analysis should also be interpreted with great caution.
authors.affiliation: Laboratory of Chemical Biology and Environmental Engineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Yokawa
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kagenishi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kawano
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancuso
authors.forename: František
authors.initials: F
authors.lastname: Baluška
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects*; D003624:Darkness; D008027:Light; D008954:Models, Biological; D018517:Plant Roots / Q000378:metabolism* / Q000528:radiation effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000254:growth & development / Q000528:radiation effects; D013194:Staining and Labeling
chemical_list: D017382:Reactive Oxygen Species