title: Immunohistochemical comparison of the expression of p53 and MDM2 proteins in ameloblastomas and keratocystic odontogenic tumors.
abstract: OBJECTIVE
  Ameloblastoma and keratocystic odontogenic tumor (KOT) is characterized by a benign but locally invasive behavior with a high risk of recurrence. MDM2 (murine double minute 2), an amplifier of cell proliferation, and p53, a tumor suppressor gene, are overexpressed in some odontogenic lesions. The aim of this study was to compare the expression of MDM2 and p53 in ameloblastoma and KOT as 2 lesions with similar biologic behavior, by immunohistochemistry.
  
  
  METHODS
  The expressions of MDM2 and p53 proteins were determined in 39 ameloblastomas (15 follicular types, 15 plexiform types, and 9 unicystic types) and 15 KOTs.
  
  
  RESULTS
  P53 protein was expressed in 100% of KOTs and 77.8% of ameloblastomas, and MDM2 was detected in 74.8% of ameloblastomas and 80% of KOTs. There was no statistical difference between MDM2 and p53 expressions in different subtypes of ameloblastomas and also when KOTs were compared with them (P > 0.05). There was a significant difference between immunohistochemical reactivity of MDM2 among subtypes of ameloblastomas (P < 0.05). MDM2 and p53 expressions were positively correlated.
  
  
  CONCLUSIONS
  Overexpression of p53 and MDM2 is associated with the pathogenesis and oncogenesis of ameloblastomas and KOT. Overexpression of these markers can contribute to similar biologic behavior of these lesions.
authors.affiliation: Anatomoclinical Pathology Department, Quaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Noorieh
authors.initials: N
authors.lastname: Sharifi-Sistani
authors.forename: Hamed
authors.initials: H
authors.lastname: Zartab
authors.forename: Shahab
authors.initials: S
authors.lastname: Babakoohi
authors.forename: Nasrollah
authors.initials: N
authors.lastname: Saghravanian
authors.forename: Shokoofeh
authors.initials: S
authors.lastname: Jamshidi
authors.forename: Habibollah
authors.initials: H
authors.lastname: Esmaili
authors.forename: Nooshin
authors.initials: N
authors.lastname: Mohtasham
authors.forename: Maryam
authors.initials: M
authors.lastname: Zamanzadeh
authors.forename: Hamid
authors.initials: H
authors.lastname: Abbaszadeh-Bidokhty
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D003430:Cross-Sectional Studies; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008207:Lymphatic Metastasis; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D009808:Odontogenic Tumors / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2