title: Cross-talk between human mesenchymal stem/progenitor cells (MSCs) and rat hippocampal slices in LPS-stimulated cocultures: the MSCs are activated to secrete prostaglandin E2.
abstract: Mesenchymal stem/progenitor cells (MSCs) improve functional outcome in a number of disease models through suppression of inflammation. However, their effects on neuroinflammation are unknown. In this study, we show that MSCs suppress endotoxin-induced glial activation in organotypic hippocampal slice cultures (OHSCs). Lipopolysaccharide-stimulated OHSCs activated MSCs to increase the expression of cyclo-oxygenase-2 and produce prostaglandin E2. MSC-derived prostaglandin E2, then suppressed pro-inflammatory cytokine production by the OHSCs. Together, the results suggest the potential anti-inflammatory mechanism of MSCs in models of disease and support earlier observations that MSCs may offer a therapy for neuroinflammation produced by trauma or disease.
authors.affiliation: Institute for Regenerative Medicine, Texas A&M Health Science Center College of Medicine at Scott & White, Module C, Temple, Texas, USA.
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Foraker
authors.forename: Joo Youn
authors.initials: JY
authors.lastname: Oh
authors.forename: Joni H
authors.initials: JH
authors.lastname: Ylostalo
authors.forename: Ryang Hwa
authors.initials: RH
authors.lastname: Lee
authors.forename: Jun
authors.initials: J
authors.lastname: Watanabe
authors.forename: Darwin J
authors.initials: DJ
authors.lastname: Prockop
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002450:Cell Communication / Q000502:physiology*; D018920:Coculture Techniques; D051546:Cyclooxygenase 2 / Q000378:metabolism / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis / Q000502:physiology; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D005911:Gliosis / Q000378:metabolism* / Q000473:pathology*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D006801:Humans; D018836:Inflammation Mediators / Q000502:physiology*; D008070:Lipopolysaccharides / Q000502:physiology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D051546:Cyclooxygenase 2; D015232:Dinoprostone