title: PRNP genetic variability and molecular typing of natural goat scrapie isolates in a high number of infected flocks.
abstract: One hundred and four scrapie positive and 77 negative goats from 34 Greek mixed flocks were analysed by prion protein gene sequencing and 17 caprine scrapie isolates from 11 flocks were submitted to molecular isolate typing. For the first time, the protective S146 variant was reported in Greece, while the protective K222 variant was detected in negative but also in five scrapie positive goats from heavily infected flocks. By immunoblotting six isolates, including two goat flockmates carrying the K222 variant, showed molecular features slightly different from all other Greek and Italian isolates co-analysed, possibly suggesting the presence of different scrapie strains in Greece.
authors.affiliation: National Agricultural Research Foundation, Veterinary Research Institute, Thessaloniki, Greece. eirfrag@gmail.com.
authors.forename: Eirini G
authors.initials: EG
authors.lastname: Fragkiadaki
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vaccari
authors.forename: Loukia V
authors.initials: LV
authors.lastname: Ekateriniadou
authors.forename: Umberto
authors.initials: U
authors.lastname: Agrimi
authors.forename: Nektarios D
authors.initials: ND
authors.lastname: Giadinis
authors.forename: Barbara
authors.initials: B
authors.lastname: Chiappini
authors.forename: Elena
authors.initials: E
authors.lastname: Esposito
authors.forename: Michela
authors.initials: M
authors.lastname: Conte
authors.forename: Romolo
authors.initials: R
authors.lastname: Nonno
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D060005:Genotyping Techniques / Q000662:veterinary; D015511:Goat Diseases / Q000453:epidemiology / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D006041:Goats; D008969:Molecular Sequence Data; D058889:Molecular Typing / Q000662:veterinary; D011328:Prions / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012608:Scrapie / Q000453:epidemiology / Q000209:etiology / Q000235:genetics* / Q000378:metabolism; D020539:Sequence Analysis, Protein / Q000662:veterinary
chemical_list: D011328:Prions