title: Characterisation and natural variation of a dehydrin gene in Quercus petraea (Matt.) Liebl.
abstract: For the first time in sessile oak [Quercus petraea (Matt.) Liebl.], the isolation and characterisation of a full-length dehydrin gene and its promoter region, as well as its allelic variation in natural populations, is reported. Dehydrins (Dhn) are stress-related genes important for the survival of perennial plants in a seasonal climate. A full-length dehydrin gene (Dhn3) was characterised at the nucleotide level and the protein structure was modelled. Additionally, the allelic variation was analysed in five natural populations of Quercus petraea (Matt.) Liebl. sampled along an altitudinal gradient in the French Pyrenees. The analysed sequences contain typical domains of the K(n)  class of dehydrins in the coding region. Also, the 5'untranslated region (promoter) of the gene was amplified, which shows typical motifs essential for drought- and cold-responsive gene expression. Single nucleotide substitutions and indels (insertions/deletions) within the coding region determine large biochemical differences at the protein level. However, only low levels of genetic differentiation between populations from different altitudes were detectable.
authors.affiliation: Buesgen-Institute, Department of Forest Genetics and Forest Tree Breeding, University of Göttingen, Germany. bvornam1@gwdg.de
authors.forename: B
authors.initials: B
authors.lastname: Vornam
authors.forename: O
authors.initials: O
authors.lastname: Gailing
authors.forename: J
authors.initials: J
authors.lastname: Derory
authors.forename: C
authors.initials: C
authors.lastname: Plomion
authors.forename: A
authors.initials: A
authors.lastname: Kremer
authors.forename: R
authors.initials: R
authors.lastname: Finkeldey
mesh_terms: D000483:Alleles; D000531:Altitude; D019943:Amino Acid Substitution; D001483:Base Sequence; D003080:Cold Temperature; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D055864:Droughts; D005602:France; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D029963:Quercus / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; C070556:dehydrin proteins, plant