title: Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria.
abstract: A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (α-hemolysin) and lipases (phospholipase A(2)). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5α in a prototype dressing.
authors.affiliation: Department of Chemistry, University of Bath, Bath BA2 7AY, United Kingdom.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
authors.forename: Thet Naing
authors.initials: TN
authors.lastname: Tun
authors.forename: Sung-ha
authors.initials: SH
authors.lastname: Hong
authors.forename: June D
authors.initials: JD
authors.lastname: Mercer-Chalmers
authors.forename: Maisem
authors.initials: M
authors.lastname: Laabei
authors.forename: Amber E R
authors.initials: AE
authors.lastname: Young
authors.forename: A Tobias A
authors.initials: AT
authors.lastname: Jenkins
mesh_terms: D058491:Bacterial Load / Q000295:instrumentation* / Q000379:methods; D001458:Bandages; D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D010865:Pilot Projects; D014946:Wound Infection / Q000175:diagnosis / Q000382:microbiology* / Q000628:therapy*