title: The effects of nicotine on vascular smooth muscle cell chemotaxis induced by thrombospondin-1 and fibronectin.
abstract: BACKGROUND
  Vascular smooth muscle cell (VSMC) migration is an important process in many vascular disorders. Nicotine, thrombospondin-1 (TSP-1) and fibronectin (Fn) separately induce VSMC migration. The hypothesis of this study was that nicotine treatment of vascular cells would augment TSP-1-induced and Fn-induced VSMC migration.
  
  
  METHODS
  VSMCs or endothelial cells (ECs) were treated with serum-free medium or nicotine. Migration of VSMCs was assessed using a modified Boyden chemotaxis chamber to serum-free medium, TSP-1, Fn, EC basal medium, and conditioned EC medium or nicotine-treated conditioned EC medium alone or with supplemented TSP-1 or Fn.
  
  
  RESULTS
  Nicotine treatment increased VSMC chemotaxis to serum-free medium, but TSP-1 or Fn had no further effect on chemotaxis. Conditioned EC and nicotine-treated conditioned EC enhanced VSMC chemotaxis, which was further augmented by Fn supplementation.
  
  
  CONCLUSIONS
  Nicotine-stimulated EC derived factors induce VSMC migration, which is augmented by the addition of Fn.
authors.affiliation: Department of Surgery, SUNY Upstate Medical University, Syracuse, NY, USA.
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Stein
authors.forename: Keri A
authors.initials: KA
authors.lastname: Seymour
authors.forename: Kristopher G
authors.initials: KG
authors.lastname: Maier
authors.forename: Vivian
authors.initials: V
authors.lastname: Gahtan
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D005731:Ganglionic Stimulants / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D009538:Nicotine / Q000494:pharmacology*; D019700:Thrombospondin 1 / Q000502:physiology
chemical_list: D005353:Fibronectins; D005731:Ganglionic Stimulants; D019700:Thrombospondin 1; D009538:Nicotine