title: Establishment and characterization of human non-small cell lung cancer             cell lines.
abstract: Non-small cell lung cancer (NSCLC), a highly malignant tumor, is common             in China and is associated with a very poor 5-year survival rate. To better understand             the cancer biology of this disease, we report here the establishment of three             new NSCLC cell lines, SCC210011, SCC211441 and ACC212102, from the tumor tissue             of three NSCLC patients. By histological analysis, we found that all three cell             lines displayed the typical features of endothelial cancer cells. The population             doubling times of SCC210011, SCC211441 and ACC212102 cells were 42, 38 and 25             h, respectively. Our cytogenetic studies indicated that these cell lines exhibit             structural and numerical chromosomal abnormalities. Furthermore, the tumorigenicity             in nude mice was confirmed, and H&E staining results revealed that they resembled             the primary tissue. These newly established cell lines may serve as useful models             for studying the molecular pathogenesis of NSCLC.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Sun Yat-Sen University Cancer Center, Guangzhou 510060, PR China.
authors.forename: Jiangchao
authors.initials: J
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Dai
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Jiaoyu
authors.initials: J
authors.lastname: Ai
authors.forename: Tingting
authors.initials: T
authors.lastname: Zeng
authors.forename: Xueying
authors.initials: X
authors.lastname: Mao
authors.forename: Lulu
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.forename: Xin-Yuan
authors.initials: XY
authors.lastname: Guan
mesh_terms: D000368:Aged; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured