title: [Scaling of the process of biosynthesis of surfactants by Rhodococcus erythropolis EK-1 on hexadecane].
abstract: Peculiarities of synthesis of surface-active substances (SAS) are studied at periodical cultivation of Rhodococcus erythropolis EK-1 in the AK-210 fermenter on medium containing n-hexadecane. Maximum indicators of SAS synthesis (concentration of extra cellular SAS is 7.2 g/l; factor of emulsification of the cultural liquid 50%; SAS yield from the substrate 50%) have been observed at 60-70% concentration of dissolved oxygen from the saturation level with aerial oxygen (pH 8.0) fractional supply of the substrate by portions each being 0.3-0.4% every 5-6 h to a final volume concentration of 2.4% and with the use of 10% inoculate grown until mid-exponential phase on the medium with 1.0 vol % of n-hexadecane. Implementation of the process of SAS biosynthesis with the fermentation equipment provided the possibility to increase almost two-fold the amount of the synthesized SAS and reduce 3.5-fold the time of cultivation of the producer strain compared with the growth in flasks at shake-flask propagator.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: S V
authors.initials: SV
authors.lastname: Ignatenko
mesh_terms: D000473:Alkanes / Q000378:metabolism*; D019149:Bioreactors; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000379:methods*; D008823:Micelles; D010100:Oxygen / Q000378:metabolism; D012240:Rhodococcus / Q000737:chemistry / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism
chemical_list: D000473:Alkanes; D003470:Culture Media; D008823:Micelles; D013501:Surface-Active Agents; C007932:n-hexadecane; D010100:Oxygen