title: Collagen intermingled chitosan-tripolyphosphate nano/micro fibrous scaffolds for tissue-engineering application.
abstract: Scaffolds comprising a nano- and micro-fibrous architecture are promising for tissue engineering, where nanofibers act as connecting network among microfibers and provide a 3D structural environment and mechanical stability for facilitating cell attachment, proliferation and migration. In this study, a novel structure was developed with polymeric micro and nano combined fibrous architecture, which aims to mimic the native extracellular matrix for tissue regeneration. Chitosan-tripolyphosphate (TPP) microfibers were prepared by wet spinning method, where collagen solution was allowed to self-assemble into nano/microfibers and subsequently freeze-dried for obtaining this combined architecture. To ensure prolonged mechanical stability, the scaffold was cross-linked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). This polymeric nano/micro combined scaffold revealed remarkable cellular activity and cytocompatibility towards both fibroblasts and osteoblast like cells and supported improved attachment and proliferation of cells than that of bare chitosan-TPP scaffolds owing to the presence of a bioactive molecule, collagen, in the intermingled form with chitosan-TPP microfiber.
authors.affiliation: a School of Medical Science and Technology, Indian Institute of Technology , Kharagpur , 721302 , India.
authors.forename: Falguni
authors.initials: F
authors.lastname: Pati
authors.forename: Basudam
authors.initials: B
authors.lastname: Adhikari
authors.forename: Santanu
authors.initials: S
authors.lastname: Dhara
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D003094:Collagen / Q000737:chemistry; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005347:Fibroblasts / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry; D010006:Osteoblasts / Q000502:physiology; D011122:Polyphosphates / Q000737:chemistry; D016062:Porosity; D012996:Solutions; D013388:Succinimides / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D011122:Polyphosphates; D012996:Solutions; D013388:Succinimides; D003094:Collagen; D048271:Chitosan; C001426:N-hydroxysuccinimide; C005692:triphosphoric acid; D005022:Ethyldimethylaminopropyl Carbodiimide