title: Construction of a xylanase A variant capable of polymerization.
abstract: The aim of our work is to furnish enzymes with polymerization ability by creating fusion constructs with the polymerizable protein, flagellin, the main component of bacterial flagellar filaments. The D3 domain of flagellin, exposed on the surface of flagellar filaments, is formed by the hypervariable central portion of the polypeptide chain. D3 is not essential for filament formation. The concept in this project is to replace the D3 domain with suitable monomeric enzymes without adversely affecting polymerization ability, and to assemble these chimeric flagellins into tubular nanostructures. To test the feasibility of this approach, xylanase A (XynA) from B. subtilis was chosen as a model enzyme for insertion into the central part of flagellin. With the help of genetic engineering, a fusion construct was created in which the D3 domain was replaced by XynA. The flagellin-XynA chimera exhibited catalytic activity as well as polymerization ability. These results demonstrate that polymerization ability can be introduced into various proteins, and building blocks for rationally designed assembly of filamentous nanostructures can be created.
authors.affiliation: Bio-Nanosystems Laboratory, Faculty of Information Technology, Research Institute of Chemical and Process Engineering, University of Pannonia, Veszprém, Hungary.
authors.forename: Veronika
authors.initials: V
authors.lastname: Szabó
authors.forename: Adél
authors.initials: A
authors.lastname: Muskotál
authors.forename: Balázs
authors.initials: B
authors.lastname: Tóth
authors.forename: Marko D
authors.initials: MD
authors.lastname: Mihovilovic
authors.forename: Ferenc
authors.initials: F
authors.lastname: Vonderviszt
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology; D043364:Endo-1,4-beta Xylanases / Q000235:genetics / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D005408:Flagellin; D043364:Endo-1,4-beta Xylanases