title: Residues in domain III of the dengue virus envelope glycoprotein involved in cell-surface glycosaminoglycan binding.
abstract: The dengue virus (DENV) envelope (E) protein mediates virus entry into cells via interaction with a range of cell-surface receptor molecules. Cell-surface glycosaminoglycans (GAGs) have been shown to play an early role in this interaction, and charged oligosaccharides such as heparin bind to the E protein. We have examined this interaction using site-directed mutagenesis of a recombinant form of the putative receptor-binding domain III of the DENV-2E protein expressed as an MBP (maltose-binding protein)-fusion protein. Using an ELISA-based GAG-binding assay, cell-based binding analysis and antiviral-activity assays, we have identified two critical residues, K291 and K295, that are involved in GAG interactions. These studies have also demonstrated differential binding between mosquito and human cells.
authors.affiliation: Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Watterson
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia.|Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Bostjan
authors.initials: B
authors.lastname: Kobe
authors.affiliation: Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia.|Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Young
mesh_terms: D000595:Amino Acid Sequence; D002460:Cell Line; D003715:Dengue / Q000378:metabolism* / Q000821:virology; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D014759:Viral Envelope Proteins