title: Linkage mapping of the Mediterranean cypress, Cupressus sempervirens, based on molecular and morphological markers.
abstract: Gene  mapping for a Cupressus species is presented for the first time. Two  linkage maps for the Mediterranean cypress (Cupressus sempervirens)  varieties, C. sempervirens var. horizontalis and C.  sempervirens var. pyramidalis, were constructed following the  pseudo-testcross mapping strategy and employing RAPD, SCAR and morphological  markers. A total of 427 loci (425 RAPDs, two SCARs) representing parents and F(1) progeny  were screened for polymorphism with 32 random decamer and two SCAR primers. A  morphological marker defined as "crown form" was also included. Of 274  polymorphic loci, the 188 that presented Mendelian inheritance formed the  mapping dataset. Of these loci, 30% were mapped into seven linkage groups for  the horizontalis (maternal) and four linkage groups for the pyramidalis (paternal) map. The putative "crown form" locus was included in a linkage  group of both maps. The horizontalis and the pyramidalis maps  covered 160.1 and 144.5 cM, respectively, while genome length was estimated to  be 1696 cM for the former variety and 1373 cM for the latter. The four RAPD  markers most tightly linked to crown form were cloned and converted to SCARs.  Each of the cloned RAPD markers yielded two to three different sequences  behaving as co-migrating fragments. Two SCAR markers, SC-D05(432) and  SC-D09(667), produced amplified bands of the expected sizes and  maintained linkage with the appropriate phenotype, but to a lesser extent  compared to their original RAPD counterparts. These linkage maps represent a  first step towards the localization of QTLs and genes controlling crown form  and other polygenic traits in cypress.
authors.affiliation: Mediterranean Agronomic Institute of Chania, Chania, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Manescu
authors.forename: N
authors.initials: N
authors.lastname: Hamamouch
authors.forename: C
authors.initials: C
authors.lastname: Maios
authors.forename: A
authors.initials: A
authors.lastname: Harfouche
authors.forename: A G
authors.initials: AG
authors.lastname: Doulis
authors.forename: F A
authors.initials: FA
authors.lastname: Aravanopoulos
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D029781:Cupressus / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D060005:Genotyping Techniques; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D040641:Quantitative Trait Loci / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers