title: Lipid peroxidation, antioxidant activities and stress protein (HSP72/73, GRP94) expression in kidney and liver of rats under lithium treatment.
abstract: The present work was aimed at studying the effects of a subchronic lithium treatment on rat liver and kidneys, paying attention to the relationship between lithium toxicity, oxidative stress, and stress protein expression. Male rats were submitted to lithium treatment by adding 2 g of lithium carbonate/kg of food for different durations up to 1 month. This treatment led to serum concentrations ranging from 0.5 mM (day 7) to 1.34 mM (day 28) and renal insufficiency highlighted by an increase of blood creatinine and urea levels and a decrease of urea excretion. Lithium treatment was found to trigger an oxidative stress both in kidney and liver, leading to an increase of lipid peroxidation level (TBARS) and of superoxide dismutase and catalase activities. Conversely, glutathione peroxidase activity was reduced. Constitutive HSP73 (heat shock protein 73) expression was not modified by lithium treatment, whereas inducible HSP72 was down-regulated in kidney. GRP94 (glucose regulated protein 94) appeared as two isoforms of 92 and 98 kDa: the 98-kDa protein being overexpressed in kidney by lithium treatment whereas 92-kDa protein was underexpressed both in kidney and liver.
authors.affiliation: Laboratoire d'écophysiologie, Sfax, PB 802, 3018, Tunisia. riadhsv@yahoo.fr
authors.forename: Riadh
authors.initials: R
authors.lastname: Nciri
authors.forename: Mohamed Salah
authors.initials: MS
authors.lastname: Allagui
authors.forename: Ezzedine
authors.initials: E
authors.lastname: Bourogaa
authors.forename: Monji
authors.initials: M
authors.lastname: Saoudi
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Murat
authors.forename: Françoise
authors.initials: F
authors.lastname: Croute
authors.forename: Abdelfettah
authors.initials: A
authors.lastname: Elfeki
mesh_terms: D000818:Animals; D018692:Antimanic Agents / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000378:metabolism*; D003404:Creatinine / Q000097:blood; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation; D016651:Lithium Carbonate / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D051437:Renal Insufficiency / Q000139:chemically induced; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014508:Urea / Q000097:blood
chemical_list: D018692:Antimanic Agents; D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D008565:Membrane Proteins; D017392:Thiobarbituric Acid Reactive Substances; C018551:glucose-regulated proteins; D016651:Lithium Carbonate; D014508:Urea; D003404:Creatinine