title: Therapeutic effect of Saraca asoca (Roxb.) Wilde on lysosomal enzymes and collagen metabolism in adjuvant induced arthritis.
abstract: Rheumatoid arthritis is a chronic, progressive and systemic inflammatory disorder mainly affecting the synovial joints. In the present study, we evaluated the anti-arthritic effect of the methanol extract of Saraca asoca (Roxb.) Wilde., (Fabaceae) on adjuvant induced arthritis by assessing paw swelling, body weight, the levels of lysosomal enzymes, protein bound carbohydrates, serum cytokines, urinary collagen and histopathology of joints. It was found that S. asoca methanol extract at doses of 50, 100 and 200 mg/kg reduced the paw thickness and elevated the mean body weight of arthritic rats. The treatment of S. asoca showed a significant reduction in the levels of both plasma and liver lysosomal enzymes. The protein bound carbohydrates and urinary collagen contents were also decreased at a significant level by the treatment of S. asoca methanol extract. The histopathological study of the joints showed the anti-arthritic property of S. asoca which nearly normalized the histological architecture of the joints. Further, we established the anti-arthritic activity of S. asoca methanol extract by measuring the levels of cytokines in both arthritic and treated rats. The treatment of S. asoca reduced the levels of pro-inflammatory cytokines. In conclusion, S. asoca methanol extract was capable of ameliorating the conditions of arthritis in adjuvant induced arthritic rats.
authors.affiliation: Division of Ethnopharmacology, Entomology Research Institute, Loyola College, Chennai, 600 034 Tamilnadu, India. biotech.saravanan@gmail.com
authors.forename: Subramanian
authors.initials: S
authors.lastname: Saravanan
authors.forename: Narasimhan Prakash
authors.initials: NP
authors.lastname: Babu
authors.forename: Perumal
authors.initials: P
authors.lastname: Pandikumar
authors.forename: Savarimuthu
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D001835:Body Weight / Q000187:drug effects; D002241:Carbohydrates / Q000097:blood; D003094:Collagen / Q000097:blood / Q000378:metabolism* / Q000652:urine; D004487:Edema / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004798:Enzymes / Q000097:blood / Q000378:metabolism*; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D007375:Interleukin-1 / Q000097:blood; D007596:Joints / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D007375:Interleukin-1; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen