title: Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.
abstract: BACKGROUND
  The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.
  
  
  METHODS
  Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.
  
  
  CONCLUSIONS
  The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.
authors.affiliation: Division of Radiation Medicine, Department of Naval Medicine, 2nd Military Medical University, Shanghai, China.
authors.forename: Ming-juan
authors.initials: MJ
authors.lastname: Li
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Shi-wei
authors.initials: SW
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.forename: Rui
authors.initials: R
authors.lastname: Min
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000378:metabolism; D003037:Cobalt Radioisotopes / Q000008:administration & dosage; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007962:Leukocytes / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011874:Radiometry / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: C493957:Cdkn1a protein, mouse; D018797:Cell Cycle Proteins; D003037:Cobalt Radioisotopes; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D064007:Ataxia Telangiectasia Mutated Proteins; C576325:Atm protein, mouse; D017346:Protein Serine-Threonine Kinases