title: Different forms of administration of biotherapy 7dH in mice experimentally infected by Trypanosoma cruzi produce different effects.
abstract: OBJECTIVE
  To evaluate the effects of different forms of administration of the blood trypomastigotes biotherapy 7dH in mice experimentally infected with Trypanosoma cruzi.
  
  
  METHODS
  Male swiss mice were inoculated with 1400 blood trypomastigotes of the Y strain of T. cruzi and allocated into 5 treatment groups: IC (distilled water); TCBZ (benznidazole); TBA(7dH) (biotherapy 7dH 20 days after infection); TBB(7dH)7 (biotherapy 7dH seven days before infection); TBB(7dH)30 (biotherapy 7dH 30 days before infection). Parasitological parameters assessed included pre-patent and patent periods, parasitemia peak, total parasitemia, mortality and survival rates. Cure index was obtained by fresh blood examination, hemoculture and polymerase chain reaction (PCR).
  
  
  RESULTS
  The TBB(7dH)7 group showed a reduction in parasitemia peak, parasitemia area under the curve and total parasitemia. TBB(7dH)30 showed a tendency to increased pre-patent and survival periods, peak parasitemia was increased without increased total parasitemia. TBA(7dH) did not present significant alterations in the parasitological parameters analyzed.
  
  
  CONCLUSIONS
  Biotherapy 7dH given before infection (7 or 30 days) produces different effects suggesting modulation of the host's immune system. The effects range from reduced parasitemia to its effective increase. The use of biotherapy to treat T. cruzi infection including dose, potency and schedule deserves further investigation.
authors.affiliation: Basic Health Science Department, Universidade Estadual de Maringá, Paraná, Brazil. fabiana_nabarro@hotmail.com
authors.forename: Fabiana Nabarro
authors.initials: FN
authors.lastname: Ferraz
authors.forename: Geysa Karla
authors.initials: GK
authors.lastname: Simoni
authors.forename: Anélio
authors.initials: A
authors.lastname: do Nascimento
authors.forename: Carolina Sundin
authors.initials: CS
authors.lastname: de Melo
authors.forename: Denise Lessa
authors.initials: DL
authors.lastname: Aleixo
authors.forename: Mônica Lúcia
authors.initials: ML
authors.lastname: Gomes
authors.forename: Miguel
authors.initials: M
authors.lastname: Spack
authors.forename: Silvana Marques
authors.initials: SM
authors.lastname: de Araújo
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy* / Q000469:parasitology; D004195:Disease Models, Animal; D006705:Homeopathy; D008297:Male; D051379:Mice; D009593:Nitroimidazoles / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C009999:benzonidazole