title: The effect of auto-vaccination therapy on the phenotypic variation of one clonal type of Staphylococcus aureus isolated from cows with mastitis.
abstract: The aim of this study was to demonstrate the effect of auto-vaccine therapy on selected properties of Staphylococcus aureus strains, isolated from milk of cows with subclinical mastitis. The experiment was based on auto-vaccines which were prepared from S. aureus strains isolated from 16 cows. S. aureus strains isolated from cows on the 7th, 21st and 35th day following auto-vaccination, were analyzed phenotypically and genotypically. The isolated strains represented 17 biotypes all belonging to one clonal type. Increases of new biotypes of S. aureus were detected on the 35th day of therapy. Among 48 re-isolated strains, 18.75% (9/48) revealed single and 12.50% (6/48) multiple phenotypical changes. The present study demonstrated that during auto-vaccine therapy, S. aureus strains can change phenotypically, pointing out the necessity for using precise diagnostic methods, that would make possible a better assessment of the used therapy.
authors.affiliation: Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Doktora Judyma Street 24, 71-466 Szczecin, Poland. pawel.nawrotek@zut.edu.pl
authors.forename: Paweł
authors.initials: P
authors.lastname: Nawrotek
authors.forename: Danuta
authors.initials: D
authors.lastname: Czernomysy-Furowicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Borkowski
authors.forename: Karol
authors.initials: K
authors.lastname: Fijałkowski
authors.forename: Anna
authors.initials: A
authors.lastname: Pobucewicz
mesh_terms: D000818:Animals; D054991:Autovaccines / Q000627:therapeutic use*; D002417:Cattle; D005260:Female; D008414:Mastitis, Bovine / Q000188:drug therapy / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D010641:Phenotype; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D013209:Staphylococcal Vaccines / Q000627:therapeutic use*; D013211:Staphylococcus aureus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D054991:Autovaccines; D013209:Staphylococcal Vaccines