title: IgE recognition patterns of profilin, PR-10, and tropomyosin panallergens tested in 3,113 allergic patients by allergen microarray-based technology.
abstract: BACKGROUND
  IgE recognition of panallergens having highly conserved sequence regions, structure, and function and shared by inhalant and food allergen sources is often observed.
  
  
  METHODS
  We evaluated the IgE recognition profile of profilins (Bet v 2, Cyn d 12, Hel a 2, Hev b 8, Mer a 1, Ole e 2, Par j 3, Phl p 12, Pho d 2), PR-10 proteins (Aln g 1, Api g 1, Bet v 1.0101, Bet v 1.0401, Cor a 1, Dau c 1 and Mal d 1.0108) and tropomyosins (Ani s 3, Der p 10, Hel as 1, Pen i 1, Pen m 1, Per a 7) using the Immuno-Solid phase Allergen Chip (ISAC) microarray system. The three panallergen groups were well represented among the allergenic molecules immobilized on the ISAC. Moreover, they are distributed in several taxonomical allergenic sources, either close or distant, and have a route of exposure being either inhalation or ingestion.
  
  
  RESULTS
  3,113 individuals (49.9% female) were selected on the basis of their reactivity to profilins, PR-10 or tropomyosins. 1,521 (48.8%) patients were reactive to profilins (77.6% Mer a 1 IgE(+)), 1,420 (45.6%) to PR-10 (92.5% Bet v 1 IgE(+)) and 632 (20.3%) to tropomyosins (68% Der p 10 IgE(+)). A significant direct relationship between different representative molecules within each group of panallergens was found. 2,688 patients (86.4%) recognized only one out of the three distinct groups of molecules as confirmed also by hierarchical clustering analysis.
  
  
  CONCLUSIONS
  Unless exposed to most of the allergens in the same or related allergenic sources, a preferential IgE response to distinct panallergens has been recorded. Allergen microarray IgE testing increases our knowledge of the IgE immune response and related epidemiological features within and between homologous molecules better describing the patients' immunological phenotypes.
authors.affiliation: Center for Molecular Allergology, IDI-IRCCS, Rome, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Scala
authors.forename: Claudia
authors.initials: C
authors.lastname: Alessandri
authors.forename: Paola
authors.initials: P
authors.lastname: Palazzo
authors.forename: Debora
authors.initials: D
authors.lastname: Pomponi
authors.forename: Marina
authors.initials: M
authors.lastname: Liso
authors.forename: Maria Livia
authors.initials: ML
authors.lastname: Bernardi
authors.forename: Rosetta
authors.initials: R
authors.lastname: Ferrara
authors.forename: Danila
authors.initials: D
authors.lastname: Zennaro
authors.forename: Mario
authors.initials: M
authors.lastname: Santoro
authors.forename: Chiara
authors.initials: C
authors.lastname: Rasi
authors.forename: Adriano
authors.initials: A
authors.lastname: Mari
mesh_terms: D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D000368:Aged; D000369:Aged, 80 and over; D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007223:Infant; D008875:Middle Aged; D010940:Plant Proteins / Q000276:immunology*; D051304:Profilins / Q000276:immunology*; D040081:Protein Array Analysis / Q000379:methods*; D011506:Proteins / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C096695:Api g 1 protein, Apium graveolens; C061277:Cor a I protein, hazel; C119925:Dau c 1 protein, Daucus carota; D010940:Plant Proteins; D051304:Profilins; D011506:Proteins; D014335:Tropomyosin; D007073:Immunoglobulin E