title: Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells.
abstract: OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) was used to identify OCT4 binding sites in glioblastoma cancer cells. The results showed that 5438 OCT4 binding sites were localized in the glioblastoma cancer genome and that these sites contained a consensus sequence TTTkswTw (k=T or G, s=C or G, w=A or T), which occurred 3931 times in 2312 OCT4 binding regions. Furthermore, binding motifs of some other transcription factors were identified in OCT4 binding regions. Our results provide a valuable dataset for understanding gene regulation mechanisms underlying the function of OCT4 in glioblastoma cancer.
authors.affiliation: Key Laboratory of Cancer Prevention and Intervention of Ministry of Education, Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Fang
authors.forename: Wei-yi
authors.initials: WY
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Dong
authors.initials: D
authors.lastname: Ding
authors.forename: Xu
authors.initials: X
authors.lastname: Hong
authors.forename: Li-sha
authors.initials: LS
authors.lastname: Li
authors.forename: Hua-rong
authors.initials: HR
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
authors.forename: Biao-yang
authors.initials: BY
authors.lastname: Lin
mesh_terms: D000918:Antibody Specificity; D001665:Binding Sites; D001932:Brain Neoplasms / Q000209:etiology* / Q000235:genetics; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D015894:Genome, Human; D005909:Glioblastoma / Q000209:etiology* / Q000235:genetics; D006801:Humans; D050814:Octamer Transcription Factor-3 / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D014157:Transcription Factors