title: Application of quantitative 19F and 1H NMR for reaction monitoring and in situ yield determinations for an early stage pharmaceutical candidate.
abstract: Quantitative NMR spectrometry (qNMR) is an attractive, viable alternative to traditional chromatographic techniques. It is a fast, easy, accurate, and nondestructive technique which allows an analyst to gain quantitative information about a component mixture without the necessity of authentic reference materials, as is the case with most other analytical techniques. This is ideal for the synthesis of active pharmaceutical ingredients (API) that are in the early stages of development where authentic standards of the analytes may not be available. In this paper, the application of (19)F and (1)H qNMR for reaction monitoring and in situ potency determinations will be discussed for an early stage pharmaceutical candidate with several analytical challenges. These challenges include low UV absorption, low ionization, thermal instability, and lack of authentic reference standards. Quantitative NMR provided quick, fit-for-purpose solutions for process development where conventional separation techniques were limited.
authors.affiliation: Chemical Research and Development, Pfizer Global Research and Development, Groton, Connecticut 06340, United States.
authors.forename: Nga M
authors.initials: NM
authors.lastname: Do
authors.forename: Mark A
authors.initials: MA
authors.lastname: Olivier
authors.forename: John J
authors.initials: JJ
authors.lastname: Salisbury
authors.forename: Carrie B
authors.initials: CB
authors.lastname: Wager
mesh_terms: D005461:Fluorine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000138:chemical synthesis; D011522:Protons
chemical_list: D004364:Pharmaceutical Preparations; D011522:Protons; D005461:Fluorine