title: Evaluation of the effects of gonadotropin-releasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis.
abstract: OBJECTIVE
  To investigate the effects of GnRH antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative pelvic adhesions by a visual scoring system and immunohistochemical methods in a rat uterine horn model.
  
  
  METHODS
  Controlled experimental animal study.
  
  
  METHODS
  Animal laboratory at an academic research environment.
  
  
  METHODS
  Twenty-one Wistar albino rats.
  
  
  METHODS
  Rats were randomized into three groups. One week before the operation the rats received either GnRH-ant or GnRH-a or saline solution; they then underwent surgical laparotomy, and both uterine horns were traumatized by a scalpel. Three weeks later, all rats were sacrificed and extension and severity of the adhesions in each group were scored by a visual scoring system. Adhesion tissues were evaluated immunohistochemically for vitronectin and u-PAR.
  
  
  METHODS
  Scores of extend and severity of adhesions and staining of vitronectin and u-PAR.
  
  
  RESULTS
  The extent of adhesion scores were 1.85 ± 0.86, 0.78 ± 1.05, and 0.42 ± 0.64, and the severity of adhesion scores were 1.71 ± 0.91, 0.57 ± 0.85, 0.50 ± 0.75 for control, GnRH-ant, and GnRH-a groups, respectively. The extent and severity of adhesions were significantly lower in both GnRH-ant and GnRH-a groups when compared with the control group. Adhesion extent scores in the GnRH-a group were lower than in the GnRH-ant group, but this difference was not significant. vitronectin and u-PAR staining were significantly greater in both the GnRH-ant and GnRH-a groups than in the control group.
  
  
  CONCLUSIONS
  GnRH-ant as well as GnRH-a reduced postoperative adhesion formation in a rat model. This finding was supported immunohistochemically by vitronectin and u-PAR staining.
authors.affiliation: Celal Bayar University School of Medicine, Department of Obstetrics and Gynecology, Manisa, Turkey. asligoker@gmail.com
authors.forename: Asli Goker
authors.initials: AG
authors.lastname: Tamay
authors.forename: Tevfik
authors.initials: T
authors.lastname: Guvenal
authors.forename: Serap Cilaker
authors.initials: SC
authors.lastname: Micili
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Yildirim
authors.forename: Candan
authors.initials: C
authors.lastname: Ozogul
authors.forename: Faik Mumtaz
authors.initials: FM
authors.lastname: Koyuncu
authors.forename: Semra Oruc
authors.initials: SO
authors.lastname: Koltan
mesh_terms: D000818:Animals; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006727:Hormone Antagonists / Q000494:pharmacology*; D007150:Immunohistochemistry; D016729:Leuprolide / Q000494:pharmacology*; D011183:Postoperative Complications / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D055293:Receptors, Urokinase Plasminogen Activator / Q000378:metabolism; D012720:Severity of Illness Index; D013997:Time Factors; D000267:Tissue Adhesions / Q000517:prevention & control; D014591:Uterine Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery; D019096:Vitronectin / Q000378:metabolism
chemical_list: D005300:Fertility Agents, Female; D006727:Hormone Antagonists; D055293:Receptors, Urokinase Plasminogen Activator; D019096:Vitronectin; D007987:Gonadotropin-Releasing Hormone; D016729:Leuprolide; C062876:cetrorelix