title: Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples.
abstract: BACKGROUND
  A recent study reported that an 18-analyte multiplexed plasma panel of signaling proteins differentiated Alzheimer's disease (AD) from controls. This study measured mRNA expression for nine of these promising bio-markers in 23 AD patients and 23 age- and sex-matched controls.
  
  
  METHODS
  Total RNA was isolated from PaxGene RNA tubes. Relative mRNA expression levels of CCL5 [RANTES], CSF1, ICAM1, IGFBP6, IL1A, IL3, IL8, PDGFB and TNF were determined by Q-RT-PCR, with GAPDH as housekeeping gene.
  
  
  RESULTS
  A panel of five markers (CCL5, CSF1, ICAM1, IL8, TNF) with detectable expression levels in all individuals differed between AD patients and controls (p interaction <0.10). Especially, the relative expression level of CCL5 was lower in AD patients than in controls (p<0.005). Across groups, levels of both CCL5 and TNF were correlated to CSF levels of τ (r=0.39, r=0.32), pτ-181 (r=0.38, r=0.33), and MMSE (r=-0.31, r=-0.33, all p<0.05).
  
  
  CONCLUSIONS
  The measured panel, and especially CCL5, could aid in the differentiation of AD from controls.
authors.affiliation: Alzheimer Center and Department of Neurology, VU University Medical Center, Amsterdam, The Netherlands. m.kester@vumc.nl
authors.forename: Maartje I
authors.initials: MI
authors.lastname: Kester
authors.forename: Wiesje M
authors.initials: WM
authors.lastname: van der Flier
authors.forename: Allerdien
authors.initials: A
authors.lastname: Visser
authors.forename: Marinus A
authors.initials: MA
authors.lastname: Blankenstein
authors.forename: Philip
authors.initials: P
authors.lastname: Scheltens
authors.forename: Cees B
authors.initials: CB
authors.lastname: Oudejans
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D018946:Chemokine CCL5 / Q000097:blood* / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018946:Chemokine CCL5; D012333:RNA, Messenger