title: The characteristics of VIM-1-producing Klebsiella pneumoniae from South Africa.
abstract: A study was designed to characterize a carbapenem-resistant Klebsiella pneumoniae (KPSA01) isolated from a patient in Gauteng, South Africa without recent travel outside South Africa. Molecular characterization was done using isoelectric focusing, polymerase chain reaction and sequencing for bla(VIM), bla(IMP), bla(NDM), bla(CTX-Ms), bla(OXAs), bla(TEMs), and bla(SHV), plasmid-mediated quinolone resistance determinants, multilocus sequencing typing, plasmid replicon typing, and addiction factors. KPSA01 produced VIM-1 and belonged to the newly described sequence type ST569. The plasmid that harboured bla(VIM) typed within the narrow host range IncF replicon group, contained the aadA1 gene cassette, and tested positive for the vagCD and ccdAB addiction systems. This is the first report of VIM-1-producing K. pneumoniae outside Europe. It is important that surveillance studies be undertaken in Africa to determine if VIM-1-producing K. pneumoniae are present in significant numbers.
authors.affiliation: Division of Microbiology, Calgary Laboratory Services, University of Calgary, Calgary, AB, Canada.
authors.forename: Gisele
authors.initials: G
authors.lastname: Peirano
authors.forename: Johan
authors.initials: J
authors.lastname: Moolman
authors.forename: André
authors.initials: A
authors.lastname: Pitondo-Silva
authors.forename: Johann D D
authors.initials: JD
authors.lastname: Pitout
mesh_terms: D000368:Aged; D015780:Carbapenems; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D016133:Polymerase Chain Reaction; D013019:South Africa; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D015780:Carbapenems; C417264:VIM-1 metallo-beta-lactamase; D001618:beta-Lactamases