title: MicroRNA profile of polyunsaturated fatty acid treated glioma cells reveal apoptosis-specific expression changes.
abstract: BACKGROUND
  Polyunsaturated fatty acids (PUFAs) such as γ-linolenic acid (GLA), arachidonic acid (AA) and docosahexaenoic acid (DHA) have cytotoxic action on glioma cells.
  
  
  RESULTS
  We evaluated the cytotoxic action of GLA, AA and DHA on glioma cells with specific reference to the expression of miRNAs. Relative expression of miRNAs were assessed by using high throughput nanocapillary real-time PCR. Most of the miRNA target genes that showed altered expression could be classified as apoptotic genes and were up-regulated by PUFA or temozolomide treatment, while similar treatments resulted in repression of the corresponding mRNAs, such as cox2, irs1, irs2, ccnd1, itgb3, bcl2, sirt1, tp53inp1 and k-ras.
  
  
  CONCLUSIONS
  Our results highlight involvement of miRNAs in the induction of apoptosis in glioma cells by fatty acids and temozolomide.
authors.affiliation: Functional Genomics Laboratory, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary. nora@avidinbiotech.com
authors.forename: Nóra
authors.initials: N
authors.lastname: Faragó
authors.forename: Liliána Z
authors.initials: LZ
authors.lastname: Fehér
authors.forename: Klára
authors.initials: K
authors.lastname: Kitajka
authors.forename: Undurti N
authors.initials: UN
authors.lastname: Das
authors.forename: László G
authors.initials: LG
authors.lastname: Puskás
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism*; D045744:Cell Line, Tumor; D003606:Dacarbazine / Q000031:analogs & derivatives / Q000494:pharmacology; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects; D005909:Glioblastoma / Q000188:drug therapy / Q000378:metabolism; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077204:Temozolomide; D015854:Up-Regulation / Q000187:drug effects; D017965:gamma-Linolenic Acid / Q000378:metabolism*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D017965:gamma-Linolenic Acid; D003606:Dacarbazine; D000077204:Temozolomide