title: Three-dimensional nonlinear microspectroscopy and imaging of soft condensed matter.
abstract: We report on the realization of a sensitive microspectroscopy and imaging approach based on a three-color femtosecond coherent anti-Stokes Raman scattering (CARS) technique with high spectral, time, and spatial resolution. Independently tunable, high-repetition rate optical parametric oscillators were used to attain a dynamic range of 5 orders of magnitude for time-domain CARS signal. The attained sensitivity permitted tracing the decay of weak and structurally complex Raman active modes in soft condensed matter. Application of this approach to imaging of the biological specimen shows a great potential in quantitative characterization of live biological media with an ability to access inter- and intra-molecular interactions.
authors.affiliation: Biomedical Research Center, West Virginia University, Morgantown, West Virginia 26506, USA.
authors.forename: Shan
authors.initials: S
authors.lastname: Yang
authors.forename: Robert B
authors.initials: RB
authors.lastname: Wysolmerski
authors.forename: Feruz
authors.initials: F
authors.lastname: Ganikhanov
mesh_terms: D000465:Algorithms; D004912:Erythrocytes / Q000166:cytology*; D004926:Escherichia coli / Q000166:cytology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D011137:Polystyrenes / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods
chemical_list: D011137:Polystyrenes