title: [Determination of the specificity of seric IgA produced in response to antigens of Leishmania (Leishmania) mexicana in murine leishmaniasis].
abstract: In experimental leishmaniasis, the role of antibodies is not entirely clear, as some authors consider that these proteins are not involved in protection against infection. However, histopathological studies in human and experimental leishmaniasis lesions, show plasma cell infiltrates positive for IgA and secretion of IgM, IgG and IgA could mediate the formation of immune complexes with parasite antigens or self components, favoring necrosis leading to the elimination of the parasite. In this study, we determined if the serum IgA in the murine model has specific reactivity against antigens of Leishmania (Leishmania) mexicana of diagnostic utility. To do this, we used mice either susceptible or resistant to cutaneous leishmaniasis, and demonstrated by indirect ELISA that serum IgA is elevated in susceptible mice compared with that produced by resistant mice. Although other studies in murine models show that the serum IgG from mice infected with L. (L) mexicana present cross reactivity with unrelated parasite antigens derived from Trypanosoma cruzi, the analysis of the specificity of IgA by antigens of L. (L) mexicana and T. cruzi, by Western Blot, showed that the IgA serum of mice infected with T. cruzi reacts too with antigens of L. (L) mexicana. These findings suggest that IgA may be useful for the clinical management and prognosis of the disease.
authors.affiliation: Laboratorio de Inmunología de Parasitosis (LABINPAR), Facultad de Ciencias, Universidad de los Andes, Mérida, Venezuela.
authors.forename: Mary Carmen
authors.initials: MC
authors.lastname: Pérez-Aguilar
authors.forename: Oskarina
authors.initials: O
authors.lastname: Hernández
authors.forename: Zulay
authors.initials: Z
authors.lastname: Maizo de Segnini
authors.forename: Carmen Haydee
authors.initials: CH
authors.lastname: Rojas
authors.forename: Silverio
authors.initials: S
authors.lastname: Díaz
authors.forename: Maritza
authors.initials: M
authors.lastname: Alarcón
authors.forename: Loredana
authors.initials: L
authors.lastname: Goncalves
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D060467:Disease Resistance; D004198:Disease Susceptibility; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007070:Immunoglobulin A / Q000097:blood* / Q000276:immunology; D007894:Leishmania mexicana / Q000254:growth & development / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007070:Immunoglobulin A