title: Establishment of a novel human myeloid leukemia cell line, AMU-AML1, carrying t(12;22)(p13;q11) without chimeric MN1-TEL and with high expression of MN1.
abstract: In this study, we established and analyzed a novel human myeloid leukemia cell line, AMU-AML1, from a patient with acute myeloid leukemia with multilineage dysplasia before the initiation of chemotherapy. AMU-AML1 cells were positive for CD13, CD33, CD117, and HLA-DR by flow cytometry analysis and showed a single chromosomal abnormality, 46, XY, t(12;22)(p13;q11.2), by G-banding and spectral karyotyping. Fluorescent in situ hybridization analysis indicated that the chromosomal breakpoint in band 12p13 was in the sequence from the 5' untranslated region to intron 1 of TEL and that the chromosomal breakpoint in band 22q11 was in the 3' untranslated region of MN1. The chimeric transcript and protein of MN1-TEL could not be detected by reverse-transcriptase polymerase chain reaction or Western blot analysis. However, the MN1 gene was amplified to three copies detected by array comparative genomic hybridization analysis, and the expression levels of the MN1 transcript and protein were high in AMU-AML1 cells when compared with other cell lines with t(12;22)(p13;q11-12). Our data showed that AMU-AML1 cells contain t(12;22)(p13;q11.2) without chimeric fusion of MN1 and TEL. The AMU-AML1 cells gained MN1 copies and had high expression levels of MN1. Thus, the AMU-AML1 cell line is useful for studying the biological consequences of t(12;22)(p13;q11.2) lacking chimeric MN1-TEL.
authors.affiliation: Division of Hematology, Department of Internal Medicine, Aichi Medical University, Aichi, Japan.
authors.forename: Mayuko
authors.initials: M
authors.lastname: Gotou
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hanamura
authors.forename: Hisao
authors.initials: H
authors.lastname: Nagoshi
authors.forename: Motohiro
authors.initials: M
authors.lastname: Wakabayashi
authors.forename: Natsumi
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tsunekawa
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Horio
authors.forename: Mineaki
authors.initials: M
authors.lastname: Goto
authors.forename: Shohei
authors.initials: S
authors.lastname: Mizuno
authors.forename: Miyuki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kazuto
authors.initials: K
authors.lastname: Suganuma
authors.forename: Hidesuke
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Akihito
authors.initials: A
authors.lastname: Hiramatsu
authors.forename: Masaya
authors.initials: M
authors.lastname: Watarai
authors.forename: Masato
authors.initials: M
authors.lastname: Shikami
authors.forename: Akira
authors.initials: A
authors.lastname: Imamura
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Mihara
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Taki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miwa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Taniwaki
authors.forename: Masakazu
authors.initials: M
authors.lastname: Nitta
mesh_terms: D045744:Cell Line, Tumor; D002871:Chromosome Banding; D056905:Chromosome Breakpoints; D002881:Chromosomes, Human, Pair 12; D002892:Chromosomes, Human, Pair 22; D055028:Comparative Genomic Hybridization; D015870:Gene Expression; D015973:Gene Expression Regulation, Leukemic; D023061:Gene Order; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D032681:Spectral Karyotyping; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C506524:MN1 protein, human; C417770:MN1-TEL fusion protein, human; D015514:Oncogene Proteins, Fusion; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins