title: Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis.
abstract: The efficient and timely resolution of DNA recombination intermediates is essential for bipolar chromosome segregation. Here, we show that the specialized chromosome segregation patterns of meiosis and mitosis, which require the coordination of recombination with cell-cycle progression, are achieved by regulating the timing of activation of two crossover-promoting endonucleases. In yeast meiosis, Mus81-Mms4 and Yen1 are controlled by phosphorylation events that lead to their sequential activation. Mus81-Mms4 is hyperactivated by Cdc5-mediated phosphorylation in meiosis I, generating the crossovers necessary for chromosome segregation. Yen1 is also tightly regulated and is activated in meiosis II to resolve persistent Holliday junctions. In yeast and human mitotic cells, a similar regulatory network restrains these nuclease activities until mitosis, biasing the outcome of recombination toward noncrossover products while also ensuring the elimination of any persistent joint molecules. Mitotic regulation thereby facilitates chromosome segregation while limiting the potential for loss of heterozygosity and sister-chromatid exchanges.
authors.affiliation: London Research Institute, Cancer Research UK, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK.
authors.forename: Joao
authors.initials: J
authors.lastname: Matos
authors.forename: Miguel G
authors.initials: MG
authors.lastname: Blanco
authors.forename: Sarah
authors.initials: S
authors.lastname: Maslen
authors.forename: J Mark
authors.initials: JM
authors.lastname: Skehel
authors.forename: Stephen C
authors.initials: SC
authors.lastname: West
mesh_terms: D002453:Cell Cycle; D003434:Crossing Over, Genetic; D045566:DNA, Cruciform; D006367:HeLa Cells; D045565:Holliday Junction Resolvases / Q000378:metabolism; D006801:Humans; D008540:Meiosis; D008938:Mitosis; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*
chemical_list: D045566:DNA, Cruciform; D045565:Holliday Junction Resolvases