title: Effect of surgical castration on expression of TRPM8 in urogenital tract of male rats.
abstract: Trpm8 (melastatin-related transient receptor potential member 8), a member of the transient receptor potential (TRP) superfamily, encoding a cation channel named TRPM8, has been shown to be a primary androgen-responsive gene and play an important role in prostate physiology. To investigate the expression feature of TRPM8 in urogenital tract of male rats, and whether TRPM8 was also regulated by androgen receptor in these organs, male Sprague-Dawley rats were divided into three groups of 35 animals as follows: sham-operated (SHAM), orchidectomized (ORX), orchidectomized plus DHT treatment (O + D). Organs in urogenital tract, including kidney, prostate, seminal vesicle (SV), testis, epididymis and penis, were collected at different post-castration periods. RT-PCR, real-time PCR and Western blotting were used to detect the expression of androgen receptor (AR) and trpm8 in these tissue. As a result, AR and trpm8 can be detected in all these organs at mRNA or/and protein level. The mRNA expression of trpm8 in kidney, prostate, SV and penis decreased 24 or 72 h after castration and kept decreasing in a time-dependant manner. However, treatment of dihydrotestosterone (DHT) could reverse the effect of surgical castration. Collectively, our data provide evidence that TRPM8 and AR were expressed generally in urogenital tract of male rats, and in these organs, expression of trpm8 was regulated by serum androgen.
authors.affiliation: Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Yang
authors.forename: Xinghuan
authors.initials: X
authors.lastname: Wang
authors.forename: Guangbin
authors.initials: G
authors.lastname: Zhu
authors.forename: Zhangyan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yongzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Meng
mesh_terms: D000818:Animals; D002369:Castration; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006416:Hematoxylin / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D050053:TRPM Cation Channels / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014566:Urogenital System / Q000378:metabolism* / Q000601:surgery*
chemical_list: D012333:RNA, Messenger; D011944:Receptors, Androgen; D050053:TRPM Cation Channels; C498947:Trpm8 protein, rat; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin