title: Disconnected Interacting Protein 1 binds with high affinity to pre-tRNA and ADAT.
abstract: Disconnected Interacting Protein 1 (DIP1), a member of the double-stranded RNA-binding protein family based on amino acid sequence, was shown previously to form complexes with multiple transcription factors in Drosophila melanogaster. To explore this protein further, we have undertaken sedimentation equilibrium experiments that demonstrate that DIP1-c (longest isoform of DIP1) is a dimer in solution, a characteristic common to other members of the dsRNA-binding protein family. The closest sequence identity for DIP1 is found within the dsRBD sequences of RNA editase enzymes. Consistent with this role, we demonstrate binding of DIP1-c to a potential physiological RNA target: pre-tRNA. In addition, DIP1-c was shown to interact with ADAT, a tRNA deaminase that presumably modifies pre-tRNAs. From these data, we hypothesize that DIP1 may serve an integrator role by binding its dsRNA ligand and recruiting protein partners for the appropriate metabolism of the bound RNA.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, 6100 Main St., Houston, TX 77005, USA. catanese@bcm.edu
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Catanese
authors.forename: Kathleen S
authors.initials: KS
authors.lastname: Matthews
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D006801:Humans; D051379:Mice; D011485:Protein Binding; D012322:RNA Precursors / Q000378:metabolism*; D016601:RNA-Binding Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C410062:DISCO Interacting Protein 1, Drosophila; D029721:Drosophila Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase