title: Preparation and evaluation of easy energy supply property of medium-chain fatty acids liposomes.
abstract: To develop an easy-energy-supply agent, medium-chain fatty acids (MCFAs) liposomes were prepared by thin-layer dispersion, freeze-thawing and dynamic high pressure microfluidization (DHPM)-freeze-thawing methods. Results showed that MCFAs nanoliposomes obtained by the novel method (DHPM-freeze-thawing) exhibited a smaller size (72.6 ± 4.9 nm), narrower size distribution (PDI = 0.175 ± 0.005), higher zeta potential (-41.27 ± 1.16 mV) and entrapment efficiency (45.9 ± 6.0%) compared to the other two methods. In the weight-loaded swimming test of the mice, the high-dose group of MCFAs nanoliposomes indicated a significantly longer swimming time (105 ± 31 min, p < 0.05), a lower serum urea nitrogen (839.5 ± 111.9 mg/L, p < 0.05) and blood lactic acid (5.7 ± 1.0 mmol/L, p ≤ 0.001), and a higher hepatic glycogen (15.0 ± 3.6 mg/g, p ≤ 0.001) than those of the control group (53 ± 13 min, 1153.6 ± 102.5 mg/L, 12.5 ± 1.9 mmol/L and 8.8 ± 3.3 mg/g, respectively). However, no significant difference was found between the high-dose group and MCFAs group. The results suggested that MCFAs nanoliposomes could be used as a potential easy-energy-supply agent.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Chengmei
authors.initials: C
authors.lastname: Liu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liu
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zheng
authors.forename: Shuibing
authors.initials: S
authors.lastname: Yang
authors.forename: Jiahong
authors.initials: J
authors.lastname: Su
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight; D004734:Energy Metabolism; D005227:Fatty Acids / Q000008:administration & dosage* / Q000378:metabolism*; D005615:Freezing; D006003:Glycogen / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008081:Liposomes / Q000737:chemistry*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013550:Swimming
chemical_list: D005227:Fatty Acids; D008081:Liposomes; D019344:Lactic Acid; D006003:Glycogen