title: In vitro characterization of EHV-4 gG-deleted mutant.
abstract: Equine herpesvirus 4 (EHV-4) is an important pathogen that causes respiratory tract disease in horse populations worldwide. Glycoprotein G (gG) homologs have been identified in several alphaherpesviruses as minor non-essential membrane-anchored glycoproteins. In this study, EHV-4 gG deletion mutant has been generated by using bacterial artificial chromosome technology to investigate the role of gG in viral pathogenesis. Our findings reported here revealed no significant difference between parental EHV-4 and gG-negative strain in their replication cycle in cell culture. Furthermore, virus titers and plaque formation were comparable in both viruses. It is noteworthy that these findings disagree with the previously published study describing gG deletion in another EHV-4 strain.
authors.affiliation: Department of Virology, Zagazig University, Zagazig, Egypt. wfazab@zedat.fu-berlin.de
authors.forename: Walid
authors.initials: W
authors.lastname: Azab
authors.forename: Abuelyazeed
authors.initials: A
authors.lastname: El-Sheikh
authors.forename: Azza
authors.initials: A
authors.lastname: Abdel-Gawad
mesh_terms: D000818:Animals; D006566:Herpesviridae Infections / Q000662:veterinary / Q000821:virology*; D023141:Herpesvirus 4, Equid / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D017384:Sequence Deletion; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism; D014774:Virulence; D014779:Virus Replication
chemical_list: D014759:Viral Envelope Proteins; C076629:glycoprotein G, Equid herpesvirus 4