title: Microparticle enlargement and altered surface proteins after air decompression are associated with inflammatory vascular injuries.
abstract: Studies in a murine model have shown that decompression stress triggers a progressive elevation in the number of circulating annexin V-coated microparticles derived from leukocytes, erythrocytes, platelets, and endothelial cells. We noted that some particles appeared to be larger than anticipated, and size continued to increase for ≥24 h postdecompression. These observations led to the hypothesis that inert gas bubbles caused the enlargement and particle size could be reduced by hydrostatic pressure. After demonstrating pressure-induced particle size reduction, we hypothesized that annexin V-positive particle changes associated with decompression contributed to their proinflammatory potential. Intravenous injection of naive mice with particles isolated from decompressed mice, but not control mice, caused intravascular neutrophil activation; perivascular neutrophil sequestration and tissue injuries were documented as elevations of vascular permeability and activated caspase-3. These changes were not observed if mice were injected with particles that had been subjected to hydrostatic recompression or particles that had been emulsified by incubation with polyethylene glycol telomere B surfactant. Hydrostatic pressure and surfactant incubation also altered the pattern of proteins expressed on the surface of particles. We conclude that proinflammatory events and vascular damage are due to enlargement of annexin V-coated particles and/or changes in surface marker protein pattern associated with provocative decompression. Injection of annexin V-coated particles from decompressed mice will recapitulate the pathophysiological vascular changes observed following decompression stress.
authors.affiliation: Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104-6068, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Tatyana N
authors.initials: TN
authors.lastname: Milovanova
authors.forename: Marina
authors.initials: M
authors.lastname: Bogush
authors.forename: Günalp
authors.initials: G
authors.lastname: Uzun
authors.forename: Veena M
authors.initials: VM
authors.lastname: Bhopale
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Thom
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D003664:Decompression / Q000009:adverse effects* / Q000379:methods; D007249:Inflammation / Q000097:blood / Q000209:etiology; D008565:Membrane Proteins / Q000097:blood*; D051379:Mice; D010316:Particle Size; D057772:Vascular System Injuries / Q000097:blood* / Q000209:etiology
chemical_list: D017304:Annexin A5; D015415:Biomarkers; D008565:Membrane Proteins