title: Preparation and immunological effectiveness of a swine influenza DNA vaccine encapsulated in chitosan nanoparticles.
abstract: Preparation conditions of a DNA vaccine against swine influenza encapsulated in chitosan nanoparticles were determined. The nanoparticles were prepared according to a complex coacervation method using chitosan as a biodegradable matrix forming polymer. Under the preparation conditions, chitosan nanoparticles containing the DNA vaccine were produced with good morphology, high encapsulation rate and high stability. Transfection test indicated that the vaccine could be expressed as an antigen in cells, and maintained good bioactivity. In addition, better immune responses of mice immunized with the chitosan nanoparticles containing the DNA vaccine were induced and prolonged release of the plasmid DNA was achieved compared to the DNA vaccine alone. These results laid a foundation for further development of DNA vaccines in nanoparticles before ultimate industrial application.
authors.affiliation: College of Life Science, Heilongjiang University, Harbin, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Xingming
authors.initials: X
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Haixia
authors.initials: H
authors.lastname: Wei
authors.forename: Qingshen
authors.initials: Q
authors.lastname: Sun
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000284:Administration, Oral; D054366:Agouti Signaling Protein; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation; D048271:Chitosan / Q000008:administration & dosage* / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D006385:Hemagglutination Inhibition Tests; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology* / Q000493:pharmacokinetics
chemical_list: D054366:Agouti Signaling Protein; D000914:Antibodies, Viral; D004337:Drug Carriers; D007252:Influenza Vaccines; D019444:Vaccines, DNA; C515183:a protein, mouse; D048271:Chitosan