title: [Molecular cloning and functional characterization of a novel gene encoding human prostaglandin carrier, hPrC].
abstract: In the present study, we isolated and determined the pharmacological characteristics of a novel gene encoding the human prostaglandin carrier (hPrC). The isolated cDNA consisted of 1431 base pairs that encoded a 477-amino acid protein, and we found that isolated hPrC does not belong to any drug transporter families. RT-PCR analysis revealed that the hPrC mRNA is expressed in various human tissues ubiquitously. When expressed in Xenopus laevis oocytes, hPrC mediated the transport of [(3)H]prostaglandin E(2) (PGE(2)) in a sodium-independent manner. The uptake of [(3)H] PGE(2) was not trans-stimulated by PG analogous. Although there are several PG transporters such as multidrug resistance-associated protein 4 (MRP4), organic cation transporter 1 (OCT1) [solute carrier (SLC) 22A1], organic anion transporter 1-3 (OAT1-3) [SLC22A6-8], OAT4 [SLC11], OATP-1 (LST-1) [SLCO1B1], OATP2B1 [SLCO2B1], OATP2A1 (PGT) [SLCO2A1], OATP4A1 (OATP-E) [SLCO4A1] have been isolated and well characterized, our findings suggest that hPrC functions as a novel transport peptide responsible for PG uptake. Our results should provide insight into the novel mechanism of the PG transport in the human body.
authors.affiliation: Department of Clinical Pharmacy, School of Pharmacy, Showa University, Tokyo, Japan.
authors.forename: Yasuna
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Junko
authors.initials: J
authors.lastname: Nojima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohbayashi
authors.forename: Noriko
authors.initials: N
authors.lastname: Kohyama
authors.forename: Toshinori
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D015723:Gene Library; D006801:Humans; D027361:Organic Anion Transporters / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D010920:Placenta; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013997:Time Factors; D014982:Xenopus laevis
chemical_list: D018076:DNA, Complementary; D027361:Organic Anion Transporters; C503526:SLCO2A1 protein, human; D015232:Dinoprostone