title: [Cloning and expression of a outer membrance protein gene(OmpS(2);) of Edwardsiella tarda HB01 and its immunogenicity].
abstract: OBJECTIVE
  To construct a recombinant strain BL21(DE3)(pET-28a-OmpS(2);),  and obtain a genetic engineering vaccine to provide protective immunity against diseases caused by Edwardsiella tarda.
  
  
  METHODS
  According to the GenBank sequences (GenBank Accession No. AY078509), one pair of primers was designed and the outer membrance protein gene (OmpS(2);) of Edwardsiella tarda HB01 was amplified by PCR.  The OmpS(2); gene was cloned in pET-28a vector and transformed into Escherichia coli BL21(DE3). The OmpS(2); protein was highly expressed when the recombinant strain BL21 (DE3) (pET-28a-OmpS(2);) was induced by IPTG. The expressed protein was 47 kD as estimated by 150 g/L SDS-polyacrylamide gel electrophoresis (SDS-PAGE).
  
  
  RESULTS
  The immunogenicity of the expressed OmpS(2); protein was confirmed by Western blotting. Mice and Paralichthys olivaceus were immunized with the genetic engineering vaccines of Edwardsiella tarda and Aeromonas hydrophila,  showing promise that all these vaccines have a high protective ability.  And the protective ability to Edwardsiella tarda and Aeromonas hydrophila in Paralichthys olivaceus respectively reached  70% and 80%.
  
  
  CONCLUSIONS
  The recombinant strain BL21 (DE3)(pET-28a-OmpS(2);) could be candidate of genetic engineering vaccine to provide protective immunity against diseases caused by Edwardsiella tarda.
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang, China.
authors.forename: Ya-ning
authors.initials: YN
authors.lastname: Zhang
authors.forename: Xiao-yue
authors.initials: XY
authors.lastname: Li
authors.forename: Xiao-na
authors.initials: XN
authors.lastname: Geng
authors.forename: Bao-hua
authors.initials: BH
authors.lastname: Zhao
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003001:Cloning, Molecular; D020609:Edwardsiella tarda / Q000235:genetics* / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000517:prevention & control; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000517:prevention & control; D051379:Mice
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines