title: Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges.
abstract: Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.
authors.affiliation: The Physiological Laboratory; University of Liverpool; Liverpool, UK.
authors.forename: Liam P
authors.initials: LP
authors.lastname: Cheeseman
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Booth
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Hood
authors.forename: Ian A
authors.initials: IA
authors.lastname: Prior
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Royle