title: [Laboratory diagnosis of HIV/AIDS patients complicated with Pneumocystis jirovecii pneumonia].
abstract: Pneumocystis jirovecii was detected in sputum samples and bronchoalveolar lavage fluid (BALF) obtained from HIV/AIDS patients complicated with Pneumocystis jirovecii pneumonia by Giemsa staining. CD4+ T lymphocytes of 500 patients were counted by flow cytometer. P. jirovecii positive rate in sputum samples (46.8%, 845/1 806) significantly lower than that of BALF (55.8%, 10(6)/190) (P < 0.05). The proportion of patients developing clinical symptoms in P. jirovecii positive cases (96.6%, 816/845) was higher than that of P. jirovecii negative cases (64.0%, 615/961) (P < 0.05). P. jirovecii positive rate increased with the decrease of CD4+ T lymphocyte number. P. jirovecii positive rates in cases with CD4+ > 200 x 10(6)/L, CDC 200 x 10(6)/L-100 x 10(6)n/L, and CD4+ < 100x10(6)/12.0% (6/50), 39.0%( 39/100), 54.6% (191/350), respectively (P < 0.05). Giemsa staining is an efficient, simple and feasible method for P. jirovecii detection, relying on the experience and skill of the operator.
authors.affiliation: Department of Clinical Laboratory, Longtan Hospital, Liuzhou 545005, China.
authors.forename: Jing-Jie
authors.initials: JJ
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Han
authors.initials: H
authors.lastname: He
authors.forename: Ling-Song
authors.initials: LS
authors.lastname: Su
authors.forename: Song-Shu
authors.initials: SS
authors.lastname: Qin
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: Hu
authors.forename: Liu-Sheng
authors.initials: LS
authors.lastname: Tang
authors.forename: Shi-Li
authors.initials: SL
authors.lastname: Li
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000175:diagnosis* / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D015496:CD4-Positive T-Lymphocytes; D060085:Coinfection / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D045363:Pneumocystis carinii; D011020:Pneumonia, Pneumocystis / Q000150:complications / Q000175:diagnosis*; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling; D055815:Young Adult