title: Association between IGF2 and CYP21  gene polymorphisms and characteristics of economic interest in Nellore cattle.
abstract: We  analyzed two single nucleotide polymorphisms (SNPs) of the IGF2 and CYP21 genes in Nellore cattle participating in the Brazilian Animal Breeding  Program. The SNPs were found in exon 6 of the IGF2 (insulin-like growth  factor 2) gene (RFLP/MboII) as well as in the promoter region of the CYP21 (steroid 21-hydroxylase) gene (RFLP/HpaII) of these animals. The TC  heterozygotes were significantly more frequent than CC and TT homozygotes in  the RFLP/MboII polymorphism. The T allele was significantly more  frequent than the C allele in RFLP/HpaII polymorphism. This population  was found to be in Hardy-Weinberg equilibrium for these SNPs. Association of  these polymorphisms with expected progeny differences of reproductive and  productive traits was investigated, but proved to be significant only for DP550  (expected progeny differenced for weight at 365 days - IGF2 - RFLP/MboII)  and DP450 (expected progeny differenced for weight at 450 days - CYP21 -  RFLP/HpaII). This is the first study on the occurrence of these two  polymorphisms in this Zebu breed of cattle. A total of 147 Nellore animals participating  in the Breeding Program of the Nellore Breed (PMGRN) under the management of  the National Association of Breeders and Researchers (ANCP) in the city of  Ribeirão Preto were analyzed.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Martins da Silva
authors.forename: A F L
authors.initials: AF
authors.lastname: Rios
authors.forename: E S
authors.initials: ES
authors.lastname: Ramos
authors.forename: R B
authors.initials: RB
authors.lastname: Lôbo
authors.forename: H N
authors.initials: HN
authors.lastname: Oliveira
authors.forename: M A R
authors.initials: MA
authors.lastname: de Freitas
mesh_terms: D000383:Agriculture; D000483:Alleles; D000822:Animal Husbandry; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D001835:Body Weight / Q000235:genetics; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency; D005819:Genetic Markers; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D013255:Steroid 21-Hydroxylase / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D007335:Insulin-Like Growth Factor II; D013255:Steroid 21-Hydroxylase