title: [The expression and significance of Notch1 in the human kidney tubular cell transdifferentiation induced by TGF-β(1);].
abstract: OBJECTIVE
  To explore the dynamic expression and significance of Notch1 in the human kidney tubular epithelial cell transdifferentiation (KTECT)induced by TGF-β(1);.
  
  
  METHODS
  Normal human kidney epithelial cell line (HK-2) was cultured and then divided into blank group,  and TGF-β(1);(10 ng/mL) induced group. At the 12 h,  24 h, 48 h and 72 h, the morphologic changes of HK-2 cells were observed under an inverted phase contrast microscope. The expression of α-SMA, E-cadherin and Notchl as well as their mRNA were examined by immunohisto-chemistry staining and RT-PCR respictively.
  
  
  RESULTS
  Compared with the  normal control group,  the expression levels ofα-SMA and Notchl markedly increased in TGF-β(1); induced group (P<0.05), but the expression of E-cadherin obviously reduced(P<0.05). The expression of Notch1 protein and its mRNA was positively correlated to the expression ofα-SMA(r(protein);=0.958; r(mRNA);=0.944; P<0.05), and was negatively correlated to the expression of E-cadherin(r(protein);=-0.937; r(mRNA);=-0.921; P<0.05).
  
  
  CONCLUSIONS
  Notch1 may participate in the KTECT induced by TGF-β(1);.
authors.affiliation: Department of Nephrology, Dujiangyan Medical Center, Dujiangyan, China.
authors.forename: Lin-feng
authors.initials: LF
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Li-xin
authors.initials: LX
authors.lastname: Jin
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Ming-yong
authors.initials: MY
authors.lastname: Wang
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015820:Cadherins; D012333:RNA, Messenger; D051881:Receptor, Notch1; D053773:Transforming Growth Factor beta1