title: Comparison of two commercial extenders for cryopreservation of goat semen without sperm washing.
abstract: The objective of this study was to evaluate the effects of two commercially available semen extenders on the motility of cryopreserved goat sperm and to simplify the cryopreservation protocol. Individual goat ejaculates were split and processed in parallel for freezing in either commercially available soy-based extender (Bioxcell®) or egg yolk-based extender (Irvine TYB). Sperm quality was assessed using total and progressive sperm motility, measured by computer-assisted sperm analysis (CASA). Total motility was higher for samples processed in soy-based extender, both at pre-freeze (P = 0.002) and at post-thaw (P < 0.0001). Progressive motility was higher for semen processed in soy extender at post-thaw (P < 0.0001). Approximately 10% of samples processed in egg yolk-based extender had a large (> 50%) reduction in total motility prior to freezing. However, this type of extreme reduction in pre-freeze motility did not occur in semen samples processed in soy extender. In addition, the use of soy-based extender eliminated the need for a time-consuming sperm washing protocol. We concluded that a commercially available soy-based extender was superior to an egg yolk-based extender in preserving motility of cryopreserved goat sperm, using a two-step method.
authors.affiliation: SVF Foundation, Newport, Rhode Island, USA. droof@svffoundation.org
authors.forename: D J
authors.initials: DJ
authors.lastname: Roof
authors.forename: S
authors.initials: S
authors.lastname: Bowley
authors.forename: L L
authors.initials: LL
authors.lastname: Price
authors.forename: D J
authors.initials: DJ
authors.lastname: Matsas
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents; D004530:Egg Yolk; D006041:Goats; D008297:Male; D055101:Semen Analysis / Q000379:methods / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013025:Glycine max; D013081:Sperm Motility
chemical_list: D003451:Cryoprotective Agents