title: Transcriptional activation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) gene by TGF-β1 in porcine kidney PK-15 cells.
abstract: In this study we investigated for the first time the transcriptional regulation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) in response to TGF-β1 in porcine kidney PK-15 cells. The pST3Gal I gene was found to span about 90kb and to be composed of 8 exons including 2 exons in the 5'-untranslated region. RT-PCR analysis indicated that the induction of pST3Gal I by TGF-β1 is regulated at the transcriptional level. Functional analysis of the 5'-flanking region of the pST3Gal I gene revealed the -1257 to -976 region functions as the TGF-β1-inducible promoter and that the Smad-binding site at -1020 is crucial for TGF-β1-induced expression of pST3Gal I in PK-15 cells. In addition, the transcriptional activity of pST3Gal I induced by TGF-β1 in PK-15 cells was strongly inhibited by SIS3, which is a specific Smad-3 inhibitor. In summary, our results identified the core promoter region in the pST3Gal I promoter and demonstrated that Smad-3 binding to the Samd-3 binding site at -1020 is essential for transcriptional activation of pST3Gal I in TGF-β1-induced PK-15 cells.
authors.affiliation: Department of Biotechnology and Brain Korea 21, Center for Silver-Bio Industrialization, Dong-A University, Busan 604-714, South Korea.
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Son
authors.forename: Kwon-Ho
authors.initials: KH
authors.lastname: Song
authors.forename: Haw-Young
authors.initials: HY
authors.lastname: Kwon
authors.forename: Kyoung-Sook
authors.initials: KS
authors.lastname: Kim
authors.forename: Cheorl-Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Keon-Bong
authors.initials: KB
authors.lastname: Oh
authors.forename: Young-Kug
authors.initials: YK
authors.lastname: Choo
authors.forename: Young-Choon
authors.initials: YC
authors.lastname: Lee
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D005786:Gene Expression Regulation; D007668:Kidney / Q000201:enzymology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012799:Sialyltransferases / Q000235:genetics*; D051785:Smad Proteins / Q000378:metabolism*; D013552:Swine / Q000235:genetics*; D015533:Transcriptional Activation; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D051785:Smad Proteins; D016211:Transforming Growth Factor alpha; D012799:Sialyltransferases; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase