title: Production of carbonyl reductase by Metschnikowia koreensis.
abstract: A new strain of the yeast Metschnikowia koreensis was grown in shake flasks and a stirred bioreactor for the production of carbonyl reductase. The optimal conditions in the bioreactor for maximizing the biomass specific activity of the enzyme were found to be: a medium composed of glucose (20 g/L), peptone (5 g/L), yeast extract (5 g/L) and zinc sulfate (0.3g/L); the pH controlled at 7; the temperature controlled at 25 °C; an agitation speed of 500 rpm; and an aeration rate of 0.25 vvm. In the bioreactor, a biomass specific enzyme activity of 115.6 U/gDCW was obtained and the maximum biomass concentration was 15.3 gDCW/L. The biomass specific enzyme activity obtained in the optimized bioreactor culture was 11-fold higher than the best result achieved in shake flasks. The bioreactor culture afforded a 2.7-fold higher biomass concentration than could be attained in shake flasks.
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160 062, Punjab, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Singh
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Chisti
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D004777:Environment; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007477:Ions; D008670:Metals / Q000494:pharmacology; D055163:Metschnikowia / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D009584:Nitrogen / Q000494:pharmacology
chemical_list: D007477:Ions; D008670:Metals; D002244:Carbon; D000429:Alcohol Oxidoreductases; D005947:Glucose; D009584:Nitrogen