title: The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis.
abstract: In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L(-1) glycerol; 3 g L(-1) yeast ex tract 3 g L(-1) K(2)HPO(4); 1 g L(-1) KH(2)PO(4); temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.
authors.affiliation: Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil.
authors.forename: Janaina B
authors.initials: JB
authors.lastname: Costa
authors.forename: Daniele M
authors.initials: DM
authors.lastname: Rossi
authors.forename: Elisangela A
authors.initials: EA
authors.lastname: De Souza
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Samios
authors.forename: Fernanda
authors.initials: F
authors.lastname: Bregalda
authors.forename: Maria
authors.initials: M
authors.lastname: do Carmo Ruaro Peralba
authors.forename: Simone H
authors.initials: SH
authors.lastname: Flores
authors.forename: Marco Antônio Z
authors.initials: MA
authors.lastname: Ayub
mesh_terms: D001674:Bioelectric Energy Sources; D056804:Biofuels / Q000032:analysis*; D019149:Bioreactors; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D013696:Temperature
chemical_list: D056804:Biofuels; D006859:Hydrogen; D005990:Glycerol