title: Microarray analysis reveals increased expression of ΔNp63α in seborrhoeic keratosis.
abstract: BACKGROUND
  Seborrhoeic keratoses (SKs) are very common benign epidermal lesions without malignant potential. Ultraviolet radiation, old age and viruses are well-known risk factors for disease development. However, the pathomechanisms of SK are not fully understood.
  
  
  OBJECTIVE
  To detect and characterize the genes that are involved in the pathogenesis of SK.
  
  
  METHODS
  We performed a gene expression study using paired lesional and nonlesional skin samples from patients with SK.
  
  
  RESULTS
  We identified and validated 19 differentially expressed genes in SK. Of these 19 genes, we focused on p63 transcription factor, which plays a pivotal role in epidermal development by regulating its transcriptional programme. We found by immunofluorescence that the expression of ΔNp63α, the most abundantly expressed p63 isoform, was significantly increased in SK as compared with normal skin. Moreover, siRNA-mediated knockdown of ΔNp63 led to the downregulation of 11 genes, including a member of the tensin family TNS4. Chromatin immunoprecipitation assay revealed that TNS4 was a target gene of p63.
  
  
  CONCLUSIONS
  We identified upregulated genes in SK using genome-wide cDNA microarray and elucidated the functional contribution of p63 to the disease transcriptome by gene-silencing assay. Taken together, these data may provide a novel insight into the molecular basis of these benign skin lesions.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, 28 Yongon-dong, Jongno-gu, Seoul 110-744, Korea.
authors.forename: E Y
authors.initials: EY
authors.lastname: Seo
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Cho
authors.forename: H C
authors.initials: HC
authors.lastname: Eun
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D020868:Gene Silencing / Q000502:physiology; D006801:Humans; D017492:Keratosis, Seborrheic / Q000235:genetics* / Q000378:metabolism; D008297:Male; D046228:Microarray Analysis / Q000379:methods; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D000070876:Tensins; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015854:Up-Regulation
chemical_list: D008840:Microfilament Proteins; C000603371:TNS4 protein, human; C115051:TP63 protein, human; D000070876:Tensins; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins