title: Recovery of as-yet-uncultured soil acidobacteria on dilute solid media.
abstract: A growing number of Acidobacteria strains have been isolated from environments worldwide, with most isolates derived from acidic samples and affiliated with subdivision 1. We recovered 18 Acidobacteria strains from an alkaline soil, among which 11 belonged to the previously uncultured subdivision 6. Various medium formulations were tested for their effects on Acidobacteria growth.
authors.affiliation: Laboratoire d'Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Campus de la Plaine CP 221, Boulevard du Triomphe, 1050 Brussels, Belgium. igeorge@ulb.ac.be
authors.forename: Isabelle F
authors.initials: IF
authors.lastname: George
authors.forename: Manuela
authors.initials: M
authors.lastname: Hartmann
authors.forename: Mark R
authors.initials: MR
authors.lastname: Liles
authors.forename: Spiros N
authors.initials: SN
authors.lastname: Agathos
mesh_terms: D061271:Acidobacteria / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S