title: Adipose tissue gene expression of factors related to lipid processing in obesity.
abstract: BACKGROUND
  Adipose tissue lipid storage and processing capacity can be a key factor for obesity-related metabolic disorders such as insulin resistance and diabetes. Lipid uptake is the first step to adipose tissue lipid storage. The aim of this study was to analyze the gene expression of factors involved in lipid uptake and processing in subcutaneous (SAT) and visceral (VAT) adipose tissue according to body mass index (BMI) and the degree of insulin resistance (IR).
  
  
  RESULTS
  VLDL receptor (VLDLR), lipoprotein lipase (LPL), acylation stimulating protein (ASP), LDL receptor-related protein 1 (LRP1) and fatty acid binding protein 4 (FABP4) gene expression was measured in VAT and SAT from 28 morbidly obese patients with Type 2 Diabetes Mellitus (T2DM) or high IR, 10 morbidly obese patients with low IR, 10 obese patients with low IR and 12 lean healthy controls. LPL, FABP4, LRP1 and ASP expression in VAT was higher in lean controls. In SAT, LPL and FABP4 expression were also higher in lean controls. BMI, plasma insulin levels and HOMA-IR correlated negatively with LPL expression in both VAT and SAT as well as with FABP4 expression in VAT. FABP4 gene expression in SAT correlated inversely with BMI and HOMA-IR. However, multiple regression analysis showed that BMI was the main variable contributing to LPL and FABP4 gene expression in both VAT and SAT.
  
  
  CONCLUSIONS
  Morbidly obese patients have a lower gene expression of factors related with lipid uptake and processing in comparison with healthy lean persons.
authors.affiliation: Laboratorio de Investigaciones Biomédicas del Hospital Virgen de la Victoria, Málaga (Fundación IMABIS), Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Clemente-Postigo
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Queipo-Ortuño
authors.forename: Diego
authors.initials: D
authors.lastname: Fernandez-Garcia
authors.forename: Ricardo
authors.initials: R
authors.lastname: Gomez-Huelgas
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Tinahones
authors.forename: Fernando
authors.initials: F
authors.lastname: Cardona
mesh_terms: D000328:Adult; D015992:Body Mass Index; D003176:Complement C3; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007333:Insulin Resistance; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009767:Obesity, Morbid / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism*; D055815:Young Adult
chemical_list: C038245:C3 protein, human; D003176:Complement C3; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C475476:LRP1 protein, human; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008071:Lipoprotein Lipase