title: Site-specific modification of proteins by the staudinger-phosphite reaction.
abstract: Chemoselective reactions are important tools for the modification of peptides and proteins. Thereby the modification is desired to be site specific and bioorthogonal. Here we describe the site-specific modification of azido-proteins via a Staudinger-type phosphite ligation. The reaction was carried out in aqueous system on proteins containing p-azido-phenylalanine in a single position introduced by the amber codon technique. A selective introduction of branched polyethylene scaffolds can be achieved with the application of the methodology reported herein.
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Majkut
authors.forename: Verena
authors.initials: V
authors.lastname: Böhrsch
authors.forename: Remigiusz
authors.initials: R
authors.lastname: Serwa
authors.forename: Michael
authors.initials: M
authors.lastname: Gerrits
authors.forename: Christian P R
authors.initials: CP
authors.lastname: Hackenberger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D017905:Phosphites / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D017905:Phosphites; D011506:Proteins; D011092:Polyethylene Glycols; D004247:DNA