title: [Chemiluminescence analysis of oil oxidizing bacteria Actinetobacter calcoaceticus extracts: effects of the extracts on pSoxS-lux biosensor].
abstract: A comparative H2O2-luminol- and Fe(II)-induced chemiluminescence analysis of extracts of two strains of marine oil oxidizing bacteria Actinetobacter calcoaceticus cultivated either in the presence or absence of oil was carried out. Effects of these extracts on E. coli MG1655 biosensor (pSoxS-lux) were studied. Activation of H2O2-induced chemiluminescence in the presence of oil was observed. This suggests activation of free radical lipid peroxidation. Aqueous extracts of microorganisms cultivated in the presence of oil were shown to activate reactive oxygen species production (ROS) in Fe(II)-induced chemiluminescence reaction mixture. Acetone-ethanol extracts induced antioxidative systems of both strains. Chemiluminescence analysis in a biological system carried utilizing E. coli MG1655 (pSoxS-lux) revealed that aqueous extracts of the strains cultivated in the absence of oil contained potential antioxidants.
authors.forename: I S
authors.initials: IS
authors.lastname: Sazykin
authors.forename: V N
authors.initials: VN
authors.lastname: Prokof'ev
authors.forename: V A
authors.initials: VA
authors.lastname: Chistiakov
authors.forename: M A
authors.initials: MA
authors.lastname: Sazykina
authors.forename: V V
authors.initials: VV
authors.lastname: Vnukov
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D015374:Biosensing Techniques; D002457:Cell Extracts / Q000737:chemistry* / Q000494:pharmacology; D005296:Ferrous Compounds / Q000378:metabolism; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D002457:Cell Extracts; D005296:Ferrous Compounds; D005609:Free Radicals; D010578:Petroleum; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C070724:SoxR protein, Bacteria; D008165:Luminol; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; C055198:LuxC protein, Bacteria