title: CTCF and cohesin cooperatively mediate the cell-type specific interchromatin interaction between Bcl11b and Arhgap6 loci.
abstract: CCCTC-binding factor (CTCF) is a master organizer of genome spatial organization and plays an important role in mediating extensive chromatin interactions. Circular chromosome conformation capture (4C) is a high-throughput approach that allows genome-wide screening for unknown potential interaction partners. Using a conserved CTCF binding site on the Bcl11b locus as bait, an interaction partner at the Arhgap6 locus on a different chromosome was identified by 4C. Additional experiments verified that the interchromatin interaction between the Bcl11b and Arhgap6 loci was cell-type specific, which was cooperatively mediated by CTCF and cohesin. Functional analysis showed that the interchromatin interaction partners were repressing regulatory elements. These results indicate that interaction chromatin loops regulate the expression of the relevant genes.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Ren
authors.forename: Minglei
authors.initials: M
authors.lastname: Shi
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Yang
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Wang
authors.forename: Zhihu
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D000076246:CCCTC-Binding Factor; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004843:Epistasis, Genetic; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D056426:Genetic Loci; D006657:Histones / Q000378:metabolism; D006801:Humans; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D049410:Luciferases, Renilla / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D037941:Silencer Elements, Transcriptional; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D000097722:Cohesins
chemical_list: D000076246:CCCTC-Binding Factor; D018797:Cell Cycle Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D020690:GTPase-Activating Proteins; D006657:Histones; D049409:Luciferases, Firefly; D049410:Luciferases, Renilla; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D000097722:Cohesins; C109971:ARHGAP6 protein, human; C479740:BCL11B protein, human; C000619697:CTCF protein, human