title: Study of the adhesion of neurodegenerative proteins on plasma-modified and coated polypropylene surfaces.
abstract: The inner polymeric surface of an ELISA titration well is plasma-modified and coated with different surfactant molecules. The titration of neurodegenerative proteins markers (prion, Tau and β-synuclein), previously demonstrated as more efficient with such modified tubes, is related to the adhesion behaviour of these proteins and their corresponding capture antibodies. The adhesion process is studied in terms of anchoring and specific mechanisms. The proteins and antibodies binding onto such modified surfaces is related to the substrate hydrophilic character calculated from the angle contact measure, to the polymer surface charge measured through the streaming potential determination at different pH and the inner surface roughness determined from AFM images. Furthermore, the influence of the blocking agent used during the ELISA titration is also studied.
authors.affiliation: a LUNAM Universite, Laboratoire Polymères, Colloides et Interfaces, UMR CNRS 6120, Université du Maine , av. O. Messiaen , 72085 , Le Mans , France.
authors.forename: F
authors.initials: F
authors.lastname: Poncin-Epaillard
authors.forename: C
authors.initials: C
authors.lastname: Mille
authors.forename: D
authors.initials: D
authors.lastname: Debarnot
authors.forename: W
authors.initials: W
authors.lastname: Zorzi
authors.forename: B
authors.initials: B
authors.lastname: El Moualij
authors.forename: A
authors.initials: A
authors.lastname: Coudreuse
authors.forename: G
authors.initials: G
authors.lastname: Legeay
authors.forename: I
authors.initials: I
authors.lastname: Quadrio
authors.forename: A
authors.initials: A
authors.lastname: Perret-Liaudet
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D011126:Polypropylenes / Q000737:chemistry*; D011328:Prions / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D051844:alpha-Synuclein / Q000737:chemistry*; D016875:tau Proteins / Q000737:chemistry*
chemical_list: D011126:Polypropylenes; D011328:Prions; D011994:Recombinant Proteins; D013501:Surface-Active Agents; D051844:alpha-Synuclein; D016875:tau Proteins