title: [Location of Dctn1 in the mouse testis and sperm and its role in spermiogenesis].
abstract: OBJECTIVE
  To investigate the role of dynactin 1 (Dctn1) in the process of mouse spermiogenesis.
  
  
  METHODS
  Western blot and indirect immunofluorescence were used to analyze the expression and location of Dctn1 in the mouse testis and spermatozoa. The highest efficiency of small interference RNA (siRNA) was verified by GC2-spd cell line in vitro and in vivo studies, respectively. Dctn1 siRNA mixed with the indicator (0.4% trypan blue) was injected into the seminiferous tubules of 3-week-old ICR mice through rete testis microinjection, and negative control siRNA injected into the control testes. The normal group included 3-week-old ICR mice that did not receive any treatment. Spermatozoa were collected from the cauda epididymis 3 weeks after siRNA injection for morphological analysis.
  
  
  RESULTS
  Dctn1 was mainly localized in the tail of spermatozoa. After interference, the sperm tail abnormality in the Dctn1 siRNA group was (23.57 +/- 0.55)%, significantly higher than (12.35 +/- 2.29)% in the control (P < 0.01, n = 3), and it was (3.37 +/- 0.69)% in the normal group.
  
  
  CONCLUSIONS
  Dctn1 plays an important role in mouse spermiogenesis, and mainly affects the formation of the tail of spermatozoa.
authors.affiliation: Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu 210029, China. mansnoopy@163.com
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Su-Ying
authors.initials: SY
authors.lastname: Li
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Zuo-Min
authors.initials: ZM
authors.lastname: Zhou
authors.forename: Jia-Hao
authors.initials: JH
authors.lastname: Sha
mesh_terms: D000818:Animals; D000072159:Dynactin Complex; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008845:Microinjections; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering; D012152:Rete Testis / Q000378:metabolism; D012671:Seminiferous Tubules / Q000378:metabolism; D013076:Sperm Count; D013081:Sperm Motility; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
chemical_list: C000606364:Dctn1 protein, mouse; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering