title: Chemical profiling of the cytotoxic triterpenoid-concentrating fraction and characterization of ergostane stereo-isomer ingredients from Antrodia camphorata.
abstract: Antrodia camphorata (AC), also known as Antrodia cinnamomea, an endemic species in Taiwan, is one of the treasured medicinal mushrooms. AC is traditionally used for its chemopreventive biofunctions. In this investigation, we report a convenient method for concentrating the antiproliferative active triterpenoid-rich fraction (FEA), from ethanolic extract of AC (EEAC). A series of stereo-isomers of zhankuic acids (1-8) from the FEA was purified by HPLC using an efficient acidic solvent system. The structures of compounds 1-8 were elucidated based on spectroscopic data analysis, and the absolute configuration of α-chiral carboxylic acid at C-25 in the structures was assigned based on reaction with (R)- and (S)-1-(9-anthryl)-2,2,2-trifluoroethanol. Major ingredients of FEA (eight ergostanes 1-8 and two lanostanes 9-10) were further characterised by high-performance liquid chromatography-photodiode array detection/mass spectrometry (HPLC-PDA/MS). Compounds 1-8 and their pair mixture forms (antcin K, antcin C, zhankuic acid C, and zhankuic acid A) were subjected to anti-proliferative assay against three human leukemia cell lines. Among them, the derivatives with carbonyl group at C-3 showed cytotoxicity with IC(50) values ranging from 16.44 to 77.04 μg/ml.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Du
authors.forename: Tung-Ying
authors.initials: TY
authors.lastname: Wu
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Wan-Yu
authors.initials: WY
authors.lastname: Lin
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Hsu
authors.forename: Fu-Ting
authors.initials: FT
authors.lastname: Cheng
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Lu
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Ya-Ting
authors.initials: YT
authors.lastname: Tsui
authors.forename: Hsuan-Lun
authors.initials: HL
authors.lastname: Chen
authors.forename: Ming-Feng
authors.initials: MF
authors.lastname: Hou
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Lu
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000363:Agaricales / Q000737:chemistry; D055491:Antrodia / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004875:Ergosterol / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D013624:Taiwan; D014270:Trifluoroethanol / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; D014270:Trifluoroethanol; D004875:Ergosterol