title: Pre-mRNA 3'-end processing complex assembly and function.
abstract: The 3'-ends of almost all eukaryotic mRNAs are formed in a two-step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly-adenosine or poly(A) tail). These reactions take place in the pre-mRNA 3' processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre-mRNA 3' processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA.
authors.forename: Serena
authors.initials: S
authors.lastname: Chan
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Choi
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D010944:Plants / Q000235:genetics / Q000378:metabolism; D039102:Poly(A)-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D011062:Polynucleotide Adenylyltransferase / Q000737:chemistry / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D026721:RNA 3' End Processing / Q000502:physiology*; D039104:RNA 3' Polyadenylation Signals; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D039221:mRNA Cleavage and Polyadenylation Factors / Q000737:chemistry / Q000378:metabolism
chemical_list: D046911:Macromolecular Substances; D039102:Poly(A)-Binding Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D039221:mRNA Cleavage and Polyadenylation Factors; D011062:Polynucleotide Adenylyltransferase; D012321:DNA-Directed RNA Polymerases