title: Cloning of an endoglycanase gene from Paenibacillus cookii and characterization of the recombinant enzyme.
abstract: An endoglycanase gene of Paenibacillus cookii SS-24 was cloned and sequenced. This Pgl8A gene had an open reading frame of 1,230 bp that encoded a putative signal sequence (31 amino acids) and mature enzyme (378 amino acids: 41,835 Da). The enzyme was most homologous to a β-1,3-1,4-glucanase of Bacillus circulans WL-12 with 84% identity. The recombinant enzyme hydrolyzed carboxymethyl cellulose, swollen celluloses, chitosan and lichenan but not Avicel, chitin powder or xylan. With chitosan as the substrate, the optimum temperature and hydrolysis products of the recombinant enzyme varied at pH 4.0 and 8.0. This is the first report that characterizes chitosanase activity under different pH conditions.
authors.affiliation: Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan. gr09803-5337@sti.chubu.ac.jp
authors.forename: Suguru
authors.initials: S
authors.lastname: Shinoda
authors.forename: Shin
authors.initials: S
authors.lastname: Kanamasa
authors.forename: Motoo
authors.initials: M
authors.lastname: Arai
mesh_terms: D002480:Cellulase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D048271:Chitosan / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D005936:Glucans / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics*; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D005936:Glucans; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D002482:Cellulose; D048271:Chitosan; D002480:Cellulase; C015968:lichenin