title: A new ion source and procedures for atmospheric pressure-electron capture dissociation of peptides.
abstract: We introduce a new atmospheric pressure-electron capture dissociation (AP-ECD) source in which conventional nanospray emitters are coupled with the source block and photoionization lamp of a PhotoSpray APPI source. We also introduce procedures for data collection and processing, aimed at maximizing the signal-to-background ratio of ECD products. Representative data from Substance P are presented to demonstrate the performance of the technique. Further, we demonstrate the effects of two important experimental variables, source temperature and vacuum-interface declustering potential (DP), on the method. Last, we show that even when a high source temperature is used to maximize efficiency, AP-ECD fragments of a model phosphorylated peptide retain the modification.
authors.affiliation: Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1, Canada. robb@chem.ubc.ca
authors.forename: Damon B
authors.initials: DB
authors.lastname: Robb
authors.forename: Jason C
authors.initials: JC
authors.lastname: Rogalski
authors.forename: Juergen
authors.initials: J
authors.lastname: Kast
authors.forename: Michael W
authors.initials: MW
authors.lastname: Blades
mesh_terms: D004583:Electrons; D004867:Equipment Design; D006358:Hot Temperature; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry; D013373:Substance P / Q000737:chemistry
chemical_list: D007477:Ions; D010455:Peptides; D010748:Phosphopeptides; D013373:Substance P