title: Nitrophenylboronic acids as highly chemoselective probes to detect hydrogen peroxide in foods and agricultural products.
abstract: Hydrogen peroxide is commonly used in the food processing industry as a chlorine-free bleaching and sterilizing agent, but excessive amounts of residual hydrogen peroxide have led to cases of food poisoning. Here we describe the development of a novel nonenzymatic colorimetric method for the determination of residual hydrogen peroxide in foods and agricultural products. Nitrophenylboronic acids chemoselectively react with hydrogen peroxide under alkaline conditions to produce yellow nitrophenolates. Of the three nitrophenylboronic acid isomers tested, the p-isomer displayed the highest sensitivity for hydrogen peroxide and the fastest reaction kinetics. The reaction product, p-nitrophenolate, has an absorption maximum at 405 nm and a good linear correlation between the hydrogen peroxide concentration and the A(405) values was obtained. We successfully applied this convenient and rapid method for hydrogen peroxide determination to samples of dried bean curds and disposable chopsticks, thereby demonstrating its potential in foods and agricultural industries.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Lu
authors.forename: Chieh-Ti
authors.initials: CT
authors.lastname: Lin
authors.forename: Ching-Ming
authors.initials: CM
authors.lastname: Chang
authors.forename: Shih-Hsiung
authors.initials: SH
authors.lastname: Wu
authors.forename: Lee-Chiang
authors.initials: LC
authors.lastname: Lo
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D001897:Boronic Acids; D005520:Food Preservatives; C017562:3-nitrobenzeneboronic acid; D006861:Hydrogen Peroxide