title: [Breast density and its association to polimorphism of the estrogen-receptor alpha gene Pvull and Xbal].
abstract: OBJECTIVE
  To study the influence of ER-alpha on mammographic density.
  
  
  METHODS
  We selected 87 healthy women (age 49.8 +/- S.D. 6.12 years). We obtained the body mass index (BMI), and a fasting blood sample for hormone determinations and DNA extraction. ER-alpha genotyping was carried out by PCR and digestion with a Pvull and Xbal restriction endonucleases. Mammographic density was assigned by the radiologist used three categories of fatty, average and dense.
  
  
  RESULTS
  Mammographic density was significantly associated with estradiol (p = 0.04), estrone (p = 0.04), and FSH (p = 0.02). The BMI was not associated with Pvull and Xbal genotypes and marginally with mammographic breast density (p = 0.06).
  
  
  CONCLUSIONS
  We did not observe compelling evidence of an association between variant alleles of genotypes estrogen receptors alpha and breast density.
authors.affiliation: Instituto de Investogaciones Médicas, Universidad de Guanajuato, México. bomo907@hotmail.com
authors.forename: Blanca
authors.initials: B
authors.lastname: Murillo Ortiz
authors.forename: Elva L
authors.initials: EL
authors.lastname: Pérez Luque
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Malacara
mesh_terms: D000328:Adult; D001940:Breast / Q000166:cytology*; D003720:Densitometry; D015252:Deoxyribonucleases, Type II Site-Specific; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008327:Mammography; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C025580:endodeoxyribonuclease XBAI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific