title: Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.
abstract: In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.
authors.affiliation: Manchester Centre for Integrative Systems Biology, The University of Manchester, Manchester, United Kingdom.
authors.forename: Kieran
authors.initials: K
authors.lastname: Smallbone
authors.forename: Naglis
authors.initials: N
authors.lastname: Malys
authors.forename: Hanan L
authors.initials: HL
authors.lastname: Messiha
authors.forename: Jill A
authors.initials: JA
authors.lastname: Wishart
authors.forename: Evangelos
authors.initials: E
authors.lastname: Simeonidis
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D057075:Enzyme Assays; D015966:Gene Expression Regulation, Fungal; D005956:Glucose-6-Phosphate Isomerase / Q000737:chemistry / Q000502:physiology; D005964:Glucosyltransferases / Q000737:chemistry / Q000502:physiology; D006019:Glycolysis; D007700:Kinetics; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D010733:Phosphoglucomutase / Q000737:chemistry / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000502:physiology; D013312:Stress, Physiological; D049490:Systems Biology; D014199:Trehalose / Q000096:biosynthesis*; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000737:chemistry / Q000502:physiology
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase