title: Mechanism of collapse of endoplasmic reticulum cisternae during African swine fever virus infection.
abstract: Infection of cells with African swine fever virus (ASFV) can lead to the formation of zipper-like stacks of structural proteins attached to collapsed endoplasmic reticulum (ER) cisternae. We show that the collapse of ER cisternae observed during ASFV infection is dependent on the viral envelope protein, J13Lp. Expression of J13Lp alone in cells is sufficient to induce collapsed ER cisternae. Collapse was dependent on a cysteine residue in the N-terminal domain of J13Lp exposed to the ER lumen. Luminal collapse was also dependent on the expression of J13Lp within stacks of ER where antiparallel interactions between the cytoplasmic domains of J13Lp orientated N-terminal domains across ER cisternae. Cisternal collapse was then driven by disulphide bonds between N-terminal domains arranged in antiparallel arrays across the ER lumen. This provides a novel mechanism for biogenesis of modified stacks of ER present in cells infected with ASFV, and may also be relevant to cellular processes.
authors.affiliation: Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
authors.forename: Miriam
authors.initials: M
authors.lastname: Windsor
authors.forename: Philippa
authors.initials: P
authors.lastname: Hawes
authors.forename: Paul
authors.initials: P
authors.lastname: Monaghan
authors.forename: Erik
authors.initials: E
authors.lastname: Snapp
authors.forename: María L
authors.initials: ML
authors.lastname: Salas
authors.forename: Javier M
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: Thomas
authors.initials: T
authors.lastname: Wileman
mesh_terms: D000358:African Swine Fever Virus / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007425:Intracellular Membranes / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010957:Plasmids; D017434:Protein Structure, Tertiary; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000648:ultrastructure; D014764:Viral Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000648:ultrastructure; D019065:Virus Assembly
chemical_list: D014759:Viral Envelope Proteins; D014764:Viral Proteins; C112404:j13L protein, African swine fever virus