title: Helichrysum gymnocephalum essential oil: chemical composition and cytotoxic, antimalarial and antioxidant activities, attribution of the activity origin by correlations.
abstract: Helichrysum gymnocephalum essential oil (EO) was prepared by hydrodistillation of its leaves and characterized by GC-MS and quantified by GC-FID. Twenty three compounds were identified. 1,8-Cineole (47.4%), bicyclosesquiphellandrene (5.6%), γ-curcumene (5.6%), α-amorphene (5.1%) and bicyclogermacrene (5%) were the main components. Our results confirmed the important chemical variability of H. gymnocephalum. The essential oil was tested in vitro for cytotoxic (on human breast cancer cells MCF-7), antimalarial (Plasmodium falciparum: FcB1-Columbia strain, chloroquine-resistant) and antioxidant (ABTS and DPPH assays) activities. H. gymnocephalum EO was found to be active against MCF-7 cells, with an IC(50) of 16 ± 2 mg/L. The essential oil was active against P. falciparum (IC(50) = 25 ± 1 mg/L). However, the essential oil exhibited a poor antioxidant activity in the DPPH (IC(50) value > 1,000 mg/L) and ABTS (IC(50) value = 1,487.67 ± 47.70 mg/L) assays. We have reviewed the existing results on the anticancer activity of essential oils on MCF-7 cell line and on their antiplasmodial activity against the P. falciparum. The aim was to establish correlations between the identified compounds and their biological activities (antiplasmodial and anticancer). β-Selinene (R² = 0.76), α-terpinolene (R² = 0.88) and aromadendrene (R² = 0.90) presented a higher relationship with the anti-cancer activity. However, only calamenene (R² = 0.70) showed a significant correlation for the antiplasmodial activity.
authors.affiliation: Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique UMR CNRS 5623, University of Toulouse, University of Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, France.
authors.forename: Samia
authors.initials: S
authors.lastname: Afoulous
authors.forename: Hicham
authors.initials: H
authors.lastname: Ferhout
authors.forename: Emmanuel Guy
authors.initials: EG
authors.lastname: Raoelison
authors.forename: Alexis
authors.initials: A
authors.lastname: Valentin
authors.forename: Béatrice
authors.initials: B
authors.lastname: Moukarzel
authors.forename: François
authors.initials: F
authors.lastname: Couderc
authors.forename: Jalloul
authors.initials: J
authors.lastname: Bouajila
mesh_terms: D000962:Antimalarials / Q000737:chemistry* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D036562:Helichrysum / Q000737:chemistry*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000962:Antimalarials; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils