title: Analysis of native biological surfaces using a 100 kV massive gold cluster source.
abstract: In the present work, the advantages of a new, 100 kV platform equipped with a massive gold cluster source for the analysis of native biological surfaces are shown. Inspection of the molecular ion emission as a function of projectile size demonstrates a secondary ion yield increase of ~100× for 520 keV Au(400)(4+) as compared to 130 keV Au(3)(1+) and 43 keV C(60). In particular, yields of tens of percent of molecular ions per projectile impact for the most abundant components can be observed with the 520 keV Au(400)(4+) probe. A comparison between 520 keV Au(400)(4+) time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) data showed a similar pattern and similar relative intensities of lipid components across a rat brain sagittal section. The abundant secondary ion yield of analyte-specific ions makes 520 keV Au(400)(4+) projectiles an attractive probe for submicrometer molecular mapping of native surfaces.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, United States.
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Fernandez-Lima
authors.forename: Jeremy
authors.initials: J
authors.lastname: Post
authors.forename: John D
authors.initials: JD
authors.lastname: DeBord
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Eller
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Verkhoturov
authors.forename: Serge
authors.initials: S
authors.lastname: Della-Negra
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods
authors.forename: Emile A
authors.initials: EA
authors.lastname: Schweikert
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D006046:Gold / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018629:Spectrometry, Mass, Secondary Ion / Q000295:instrumentation / Q000379:methods*; D013499:Surface Properties
chemical_list: D008055:Lipids; D006046:Gold