title: Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma.
abstract: OBJECTIVE
  Mitogen-activated protein kinases (MAPKs) are correlated with a more malignant phenotype in many cancers. This study was designed to evaluate the predictive value of the expression of MAPK phosphatase-1 (MKP-1) and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK(1/2)), as the key regulatory mechanism of the MAPKs, in lung squamous cell carcinoma (SCC).
  
  
  METHODS
  We assessed the expressions of MKP-1 and p-ERK(1/2) in twenty subjects at different differentiation degree of SCC and five normal lungs by immunohistochemistry and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis.
  
  
  RESULTS
  Immunohistochemistry and real-time RT-PCR assay showed that the expression of MKP-1 was gradually decreased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was negatively correlated with tumor differentiation (P<0.01). However, the expression of p-ERK(1/2) or ERK(1/2) was gradually increased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was positively correlated with tumor differentiation (P<0.01).
  
  
  CONCLUSIONS
  Our data indicates the relevance of MKP-1 and p-ERK(1/2) in SCC as a potential positive and negative prognostic factor. The imbalanced expression of MKP-1 and p-ERK(1/2) may play a role in the development of SCC and these two molecules may be new targets for the therapy and prognosis of SCC.
authors.affiliation: Department of Respiratory Medicine, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ying-ying
authors.initials: YY
authors.lastname: Qian
authors.forename: Zhe-yuan
authors.initials: ZY
authors.lastname: Ding
authors.forename: Jun-hui
authors.initials: JH
authors.lastname: Lv
authors.forename: Hua-hao
authors.initials: HH
authors.lastname: Shen
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000209:etiology*; D054638:Dual Specificity Phosphatase 1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry / Q000209:etiology*; D008297:Male; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D048052:Mitogen-Activated Protein Kinase 3 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D016133:Polymerase Chain Reaction
chemical_list: D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; C517019:DUSP1 protein, human; D054638:Dual Specificity Phosphatase 1