title: Crude polysaccharide from an anti-UVB cell clone of Bupleurum scorzonerifolium protect HaCaT cells against UVB-induced oxidative stress.
abstract: Bupleurum                         scorzonerifolium Willd has been found to have a wide range of immunopharmacologic functions. We isolated an anti-UVB B. scorzonerifolium cell clone and found elevated level of polysaccharides. In this study, we investigated the ability of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone to inhibit UVB-induced photodamage using a human skin keratinocyte cell line, HaCaT. Cells were UVB irradiated and then incubated in presence of different concentrations of CP. MTT assay showed that the CP did not induce cytotoxic effect under 10 mg/mL and after UVB irradiation, CP can inhibit UVB-induced HaCaT cell death. Decreased reactive oxygen species and lipid peroxidation and increased superoxide dismutase activity showed that CP can act as a free radical scavenger. Furthermore, CP had a strong protective ability against UVB-induced DNA damage. These effects were compared to the crude polysaccharide (CP') from normal B. scorzonerifolium callus at concentration of 20 mg/mL. The portion of crude polysaccharide (CP) from the anti-UVB B. scorzonerifolium cell clone was more than 2.5-fold higher than crude polysaccharide (CP') from normal B. scorzonerifolium callus. Taken together, the protective mechanisms of crude polysaccharide from the anti-UVB B. scorzonerifolium cell clone against UVB-induced photodamage occur by the inhibition of UVB-induced reactive oxygen species production, lipid peroxidation and DNA damage.
authors.affiliation: Plant Science Institute, School of Life Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Jinran
authors.initials: J
authors.lastname: Dai
authors.forename: Haiyin
authors.initials: H
authors.lastname: Ma
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Li
authors.forename: Jianguang
authors.initials: J
authors.lastname: Wang
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ni
authors.forename: Guangmin
authors.initials: G
authors.lastname: Xia
authors.forename: Suiyun
authors.initials: S
authors.lastname: Chen