title: High-level production of 1,3-propanediol from crude glycerol by Clostridium butyricum AKR102a.
abstract: The aim of this study was to optimize a biotechnological process for the production of 1,3-propanediol (1,3-PD) based on low-quality crude glycerol derived from biodiesel production. Clostridium butyricum AKR102a was used in fed-batch fermentations in 1-L and 200-L scale. The newly discovered strain is characterized by rapid growth, high product tolerance, and the ability to use crude glycerol at the lowest purity directly gained from a biodiesel plant side stream. Using pure glycerol, the strain AKR102 reached 93.7 g/L 1,3-PD with an overall productivity of 3.3 g/(L*h). With crude glycerol under the same conditions, 76.2 g/L 1,3-PD was produced with a productivity of 2.3 g/(L*h). These are among the best results published so far for natural producers. The scale up to 200 L was possible. Due to the simpler process design, only 61.5 g/L 1,3-PD could be reached with a productivity of 2.1 g/(L*h).
authors.affiliation: Johann Heinrich von Thünen-Institut (vTI), Institute of Agricultural Technology and Biosystems Engineering (AB), Bundesallee 50, 38116 Braunschweig, Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Wilkens
authors.forename: Anne Katrin
authors.initials: AK
authors.lastname: Ringel
authors.forename: Diana
authors.initials: D
authors.lastname: Hortig
authors.forename: Thomas
authors.initials: T
authors.lastname: Willke
authors.forename: Klaus-Dieter
authors.initials: KD
authors.lastname: Vorlop
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D046972:Clostridium butyricum / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol