title: [Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application].
abstract: OBJECTIVE
  To develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.
  
  
  METHODS
  A double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.
  
  
  RESULTS
  The sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.
  
  
  CONCLUSIONS
  The TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.
authors.affiliation: Institute of Antibody Engineering, Southern Medical University, Guangzhou, China. mqqqm2006@163.com
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: Guan-feng
authors.initials: GF
authors.lastname: Lin
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Zou
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Ying-song
authors.initials: YS
authors.lastname: Wu
mesh_terms: D003429:Cross Reactions; D015200:Fluoroimmunoassay / Q000379:methods*; D037342:Pertussis Toxin / Q000032:analysis*; D010567:Pertussis Vaccine / Q000737:chemistry / Q000592:standards; D011786:Quality Control; D012680:Sensitivity and Specificity
chemical_list: D010567:Pertussis Vaccine; C083388:pertussis toxin, S1 subunit; D037342:Pertussis Toxin