title: Stem cells in burn eschar.
abstract: This study compares mesenchymal cells isolated from excised burn wound eschar with adipose-derived stem cells (ASCs) and dermal fibroblasts in their ability to conform to the requirements for multipotent mesenchymal stem cells (MSCs). A population of multipotent stem cells in burn eschar  could be an interesting resource for tissue engineering approaches to heal burn wounds. Cells from burn eschar, dermis, and adipose tissue were assessed for relevant CD marker profiles using flow cytometry and for their trilineage differentiation ability in adipogenic, osteogenic, and chondrogenic  conditions. Although the different cell types did not differ significantly in their CD marker expression, the eschar-derived cells and ASCs readily differentiated into adipocytes, osteoblasts, and chondrocytes, while dermal fibroblasts only exhibited some chondrogenic potential. We conclude  that the eschar-derived mesenchymal cells represent a population of multipotent stem cells. The origin of the cells from burn eschar remains unclear, but it is likely they represent a population of adult stem cells mobilized from other parts of the body in response to the burn injury. Their  resemblance to ASCs could also be cause for speculation that in deep burns the subcutaneous adipose tissue might be an important stem cell source for the healing wound.
authors.affiliation: Association of Dutch Burn Centers, Beverwijk, The Netherlands.
authors.forename: Vincent C
authors.initials: VC
authors.lastname: van der Veen
authors.forename: Marcel
authors.initials: M
authors.lastname: Vlig
authors.forename: Florine J
authors.initials: FJ
authors.lastname: van Milligen
authors.forename: Sharon I
authors.initials: SI
authors.lastname: de Vries
authors.forename: Esther
authors.initials: E
authors.lastname: Middelkoop
authors.forename: Magda M W
authors.initials: MM
authors.lastname: Ulrich
mesh_terms: D000199:Actins / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000166:cytology; D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D002056:Burns / Q000473:pathology*; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D019902:Chondrocytes / Q000166:cytology; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology; D012867:Skin / Q000166:cytology*; D023822:Tissue Engineering; D014945:Wound Healing; D055815:Young Adult
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D015703:Antigens, CD; D024043:Collagen Type II