title: Suppression of vascular endothelial growth factor via siRNA interference modulates the biological behavior of human nasopharyngeal carcinoma cells.
abstract: OBJECTIVE
  The aim was to study the effect of vascular endothelial growth factor (VEGF) down-regulation by small interfering (si)RNA-mediated interference (RNAi) on the biological features of nasopharyngeal carcinoma cell line CNE-2.
  
  
  METHODS
  The combined plasmids pU-siVEGF and pU-siCONT were transfected into CNE-2 cells with lipofectamine. The transfected cells were placed in fresh medium containing G418. Expression of VEGF mRNA and protein were measured by reverse transcriptase-polymerase chain reaction and Western blot, respectively. The transwell chamber model was employed to test the ability of cell invasion in vitro. The distribution of cell cycle phases was determined by flow cytometry. Cell survival was assessed by clonogenic assays.
  
  
  RESULTS
  Both VEGF mRNA and protein expression were significantly decreased in the pU-siVEGF group compared with controls (P < 0.05). The cell cycle was arrested in the G(1) phase (P < 0.05). A higher apoptotic ratio and lower invasion ability were seen in the pU-siVEGF group. The D(0) (mean lethal dose) and SF(2) values were significantly lower than those in the control group (P < 0.05).
  
  
  CONCLUSIONS
  Delivery of siRNA targeting VEGF seems efficient in down-regulating VEGF expression and diminishing the growth, proliferation, and invasiveness of CNE-2 cells. It also enhanced the sensitivity of CNE-2 cells to radiation.
authors.affiliation: Department of Radiation and Medical Oncology, Second Hospital of Yichang, 4 Tiyuchang Road, Yichang, Hubei 443000, China. yczhbnn@yahoo.com.cn
authors.forename: Hai B
authors.initials: HB
authors.lastname: Zhou
authors.forename: Yi F
authors.initials: YF
authors.lastname: Yin
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Li Y
authors.initials: LY
authors.lastname: Zou
authors.forename: Yong J
authors.initials: YJ
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Gu
authors.forename: Bao Q
authors.initials: BQ
authors.lastname: Ou
authors.forename: Juan
authors.initials: J
authors.lastname: Fu
authors.forename: Jun H
authors.initials: JH
authors.lastname: Du
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D002277:Carcinoma; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D016009:Chi-Square Distribution; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000532:radiotherapy; D010957:Plasmids; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A