title: Bradykinin and des-Arg10-kallidin enhance the adhesion of polymorphonuclear leukocytes to extracellular matrix proteins and endothelial cells.
abstract: Bradykinin-related peptides (kinins) are well known to contribute to leukocyte recruitment to inflammatory foci; however, a role of these universal pro-inflammatory mediators in the first step of this process, i.e. the leukocyte adhesion to endothelial cells, is not well understood. In this work we found that bradykinin and des-Arg10-kallidin enhance the adhesion of polymorphonuclear bloods cells (PMN) to fibrinogen and fibronectin. Also, the PMN adherence to endothelial cells of HMEC-1 line strongly increased after stimulation by kinins, particularly des-Arg10-kallidin, or when PMN were co-stimulated with bradykinin and interleukin-1β. These effects were attenuated after PMN treatment with a specific inhibitor of carboxypeptidases, which convert kinins to their des-Arg metabolites. The kinin peptides were also able to change the Mac-1 integrin expression on the PMN surface. These results suggest a regulatory effect of kinins on leukocyte adhesion to endothelial wall, providing new aspects of the leukocyte infiltration into inflamed tissues.
authors.affiliation: Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University, Kraków, Poland. ibeth.guevara-lora@uj.edu.pl
authors.forename: Ibeth
authors.initials: I
authors.lastname: Guevara-Lora
authors.forename: Anna
authors.initials: A
authors.lastname: Labedz
authors.forename: Joanna
authors.initials: J
authors.lastname: Skrzeczynska-Moncznik
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kozik
mesh_terms: D001920:Bradykinin / Q000494:pharmacology*; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000494:pharmacology; D007609:Kallidin / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D016326:Extracellular Matrix Proteins; D053583:Interleukin-1beta; D016177:Macrophage-1 Antigen; D014665:Vasodilator Agents; D007609:Kallidin; D002268:Carboxypeptidases; D001920:Bradykinin