title: The effect of probiotics on faecal microbiota and genotoxic activity of faecal water in patients with atopic dermatitis: a randomized, placebo-controlled study.
abstract: BACKGROUND
  Colonic microbiota is involved in the etiology of colon cancer according to several reports. Studies also indicate that the microbiota differs between atopic patients and healthy subjects.
  
  
  OBJECTIVE
  To evaluate whether a probiotic mix containing Lactobacillus paracasei Lpc-37, Lactobacillus acidophilus 74-2, and Bifidobacterium animalis subsp. lactis DGCC 420 can affect the microbiota and its genotoxic activity in healthy subjects and patients with atopic dermatitis (AD).
  
  
  METHODS
  A placebo-controlled cross-over study was conducted. Fifteen healthy adults and 15 adult AD patients consumed 2×100 ml/d of either a probiotic or a placebo drink for 8 weeks followed by a wash out period of 2 weeks before crossing the intervention. Faecal water was isolated from stool samples collected at the end of each period. HT29c19a cells incubated with faecal water were measured for DNA damage using single-cell gel electrophoresis ("comet assay"). Bacterial species were determined by qPCR and concentrations of short-chain fatty acids were measured by means of gas chromatography.
  
  
  RESULTS
  Probiotic supplementation resulted in a significant increase in lactobacilli, whereas numbers of Bifidobacteria and Bacteroidetes remained unchanged. Clostridium perfringens cluster I-II was significantly reduced in healthy subjects. Genotoxic potential (expressed as tail intensity) of faecal water, was not affected. However, tail intensity decreased significantly in the probiotic period compared to placebo (23.5 vs. 16.7%) in AD patients. Although faecal concentrations of short-chain fatty acids were not affected, faecal pH was significantly reduced (7.0 vs. 6.6) in AD patients after probiotics.
  
  
  CONCLUSIONS
  The results indicate that probiotics lower the genotoxic potential of faecal water in AD patients. The faecal C. perfringens cluster I-II levels remained unaffected suggesting either a change in their activity, or the fact that other bacterial species are responsible for the reduced genotoxic activity of faecal water.
authors.affiliation: Institute of Nutrition, Department of Nutritional Physiology, Friedrich Schiller University, Dornburger Str. 24, 07743 Jena, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Roessler
authors.forename: S D
authors.initials: SD
authors.lastname: Forssten
authors.forename: M
authors.initials: M
authors.lastname: Glei
authors.forename: A C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: G
authors.initials: G
authors.lastname: Jahreis
mesh_terms: D000293:Adolescent; D000328:Adult; D001644:Bifidobacterium / Q000378:metabolism; D020552:Comet Assay / Q000379:methods; D018592:Cross-Over Studies; D004249:DNA Damage; D017931:DNA Primers / Q000235:genetics; D003876:Dermatitis, Atopic / Q000235:genetics / Q000382:microbiology*; D004311:Double-Blind Method; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007779:Lactobacillus acidophilus / Q000378:metabolism; D008297:Male; D054892:Metagenome; D019936:Probiotics / Q000008:administration & dosage*; D060888:Real-Time Polymerase Chain Reaction; D014867:Water / Q000502:physiology*; D055815:Young Adult
chemical_list: D017931:DNA Primers; D005232:Fatty Acids, Volatile; D014867:Water