title: Laser-induced carotid artery injury model in the rat for therapeutic agent screening.
abstract: The aims of this study were to establish a rat model of carotid artery injury and to evaluate its suitability for evaluating therapeutic agents active against endothelial proliferation. Wistar-Kyoto rats were injected intravenously with the photochemically reactive dyes rose bengal or Evans blue, and the carotid artery was then focally irradiated with laser light of the appropriate wavelength. Histological sections of the carotid artery were analyzed to determine the appropriate parameters for this model. Ferulic acid was used to assess the suitability of this model for drug screening. No animal died as a result of the photochemical treatment. Endothelial proliferation in the carotid artery was observed in rats injected with rose Bengal and exposed to green laser light. Ferulic acid (400 mg/kg per day) significantly (p<0.05) reduced endothelial proliferation in the carotid artery 28 days after injury in dye-treated animals compared with vehicle-treated animals. This simple experimental rat model is suitable for studying factors inhibiting endothelial thickening after vessel damage and for developing therapeutic strategies active against endothelial proliferation.
authors.affiliation: Center for Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan.
authors.forename: Chin-Hsien
authors.initials: CH
authors.lastname: Chang
authors.forename: Kuo-Ti
authors.initials: KT
authors.lastname: Chen
authors.forename: Chang-Jer
authors.initials: CJ
authors.lastname: Wu
authors.forename: Shih-Liang
authors.initials: SL
authors.lastname: Chang
authors.forename: Ya-Huang
authors.initials: YH
authors.lastname: Chiu
authors.forename: Pei-Hsiu
authors.initials: PH
authors.lastname: Hung
authors.forename: Jih-Chao
authors.initials: JC
authors.lastname: Yeh
authors.forename: Yen-Lin
authors.initials: YL
authors.lastname: Chang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D020212:Carotid Artery Injuries / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology; D005070:Evans Blue / Q000008:administration & dosage; D053844:Lasers, Solid-State / Q000009:adverse effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000473:pathology; D058426:Neointima / Q000188:drug therapy / Q000209:etiology / Q000473:pathology; D017319:Photosensitizing Agents / Q000008:administration & dosage; D051381:Rats; D011921:Rats, Inbred WKY; D012395:Rose Bengal / Q000008:administration & dosage
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003373:Coumaric Acids; D017319:Photosensitizing Agents; D012395:Rose Bengal; D005070:Evans Blue; C004999:ferulic acid