title: Membrane labeling and immobilization via copper-free click chemistry.
abstract: Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion via cyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization.
authors.affiliation: Department of Chemistry, The University of Tennessee, Knoxville, TN, 37996, USA.
authors.forename: Heidi E
authors.initials: HE
authors.lastname: Bostic
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Smith
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Poloukhtine
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Popik
authors.forename: Michael D
authors.initials: MD
authors.lastname: Best
mesh_terms: D001386:Azides / Q000737:chemistry; D057930:Click Chemistry; D034242:Cyclooctanes / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D015394:Molecular Structure; D013194:Staining and Labeling / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry
chemical_list: D001386:Azides; D034242:Cyclooctanes; D008055:Lipids; D008081:Liposomes; D019809:Streptavidin; C474479:cyclooctane