title: Cytoskeletal heart-enriched actin-associated protein (CHAP) is expressed in striated and smooth muscle cells in chick and mouse during embryonic and adult stages.
abstract: We recently identified a new Z-disc protein, CHAP (Cytoskeletal Heart-enriched Actin-associated Protein), which is expressed in striated muscle and plays an important role during embryonic muscle development in mouse and zebrafish. Here, we confirm and further extend these findings by (i) the identification and characterization of the CHAP orthologue in chick and (ii) providing a detailed analysis of CHAP expression in mouse during embryonic and adult stages. Chick CHAP contains a PDZ domain and a nuclear localization signal, resembling the human and mouse CHAPa. CHAP is expressed in the developing heart and somites, as well as muscle precursors of the limb buds in mouse and chick embryos. CHAP expression in heart and skeletal muscle is maintained in adult mice, both in slow and fast muscle fibers. Moreover, besides expression in striated muscle, we demonstrate that CHAP is expressed in smooth muscle cells of aorta, carotid and coronary arteries in adult mice, but not during embryonic development.
authors.affiliation: Leiden University Medical Center, Department of Anatomy and Embryology, Leiden, The Netherlands.
authors.forename: Willemijn
authors.initials: W
authors.lastname: van Eldik
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Beqqali
authors.forename: Jantine
authors.initials: J
authors.lastname: Monshouwer-Kloots
authors.forename: Christine
authors.initials: C
authors.lastname: Mummery
authors.forename: Robert
authors.initials: R
authors.lastname: Passier
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000378:metabolism; D030161:Avian Proteins / Q000737:chemistry / Q000378:metabolism; D002339:Carotid Arteries / Q000378:metabolism; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D003331:Coronary Vessels / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D006321:Heart / Q000196:embryology / Q000502:physiology; D018878:Limb Buds / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D024510:Muscle Development; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000196:embryology; D009130:Muscle, Smooth / Q000196:embryology / Q000378:metabolism*; D054792:Muscle, Striated / Q000196:embryology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D011247:Pregnancy; D020033:Protein Isoforms; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D019170:Somites / Q000196:embryology / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; C570518:Synpo2l protein, mouse