title: A sensitive choline biosensor using Fe3O4 magnetic nanoparticles as peroxidase mimics.
abstract: A sensitive choline biosensor using Fe(3)O(4) magnetic nanoparticles and a choline oxidase modified gold electrode was developed. Fe(3)O(4) magnetic nanoparticles as peroxidase mimics used in the choline biosensor can not only improve the sensitivity of the response signal, but also possess the favorable properties of stability, magnetic separation and easy preparation, etc. When using the reduction currents of square wave voltammetry as the detection signals, the interferences of ascorbic acid and uric acid to the choline biosensor can be reduced effectively. The reduction currents of the square wave voltammetry were increased with the logarithm values of the choline chloride concentration (from 10(-9) to 10(-2) M), the detection limit was estimated to be 0.1 nM (S/N = 3). This choline biosensor also exhibited favorable selectivity and stability in the determination of choline chloride.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors.forename: Zhanxia
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Wang
authors.forename: Xiurong
authors.initials: X
authors.lastname: Yang
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001205:Ascorbic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002794:Choline / Q000032:analysis*; D005290:Ferric Compounds; D006046:Gold; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000379:methods; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance; D014527:Uric Acid / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D014527:Uric Acid; D006046:Gold; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002794:Choline; D001205:Ascorbic Acid