title: Cyclin B1 expression and p53 status in squamous cell carcinomas of the head and neck.
abstract: BACKGROUND
  The cyclin B1/CDC2 complex governs entry into mitosis by regulating the G(2)/M checkpoint, and it can be repressed by the tumor suppressor p53. We aimed to determine cyclin B1 expression in squamous cell carcinomas of the head and neck (SCCHN) and correlate it with p53 status and clinicopathological parameters.
  
  
  METHODS
  Cyclin B1 and p53 protein expression was analyzed by immunohistochemistry, and p53 mutation analyses were performed.
  
  
  RESULTS
  Cytoplasmic expression of cyclin B1 was found in all 26 SCCHN studied. In contrast, nuclear staining was seen in the basal layers of normal mucosa. A total of 46% of tumors showed high cyclin B1 expression. p53 was overexpressed in 53.8% of cases, and of these 79% carried a p53 gene mutation. High cyclin B1 expression significantly correlated with the high tumor grade, but not with gender, tumor size, nodal status, local tumor recurrence or p53 expression.
  
  
  CONCLUSIONS
  Cyclin B1 is frequently overexpressed in SCCHN, and its high expression is significantly associated with a high tumor grade. These data suggest that cyclin B1 may serve as a potential prognostic biomarker in SCCHN.
authors.affiliation: Department of Otorhinolaryngology, University of Duisburg-Essen, Hufelandstrasse 55, 45147 Essen, Germany.  thomas.hoffmann@uk-essen.de.
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Hoffmann
authors.forename: Sokratis
authors.initials: S
authors.lastname: Trellakis
authors.forename: Kornelia
authors.initials: K
authors.lastname: Okulicz
authors.forename: Patrick
authors.initials: P
authors.lastname: Schuler
authors.forename: Jens
authors.initials: J
authors.lastname: Greve
authors.forename: Judith
authors.initials: J
authors.lastname: Arnolds
authors.forename: Christoph
authors.initials: C
authors.lastname: Bergmann
authors.forename: Murat
authors.initials: M
authors.lastname: Bas
authors.forename: Stephan
authors.initials: S
authors.lastname: Lang
authors.forename: Götz
authors.initials: G
authors.lastname: Lehnerdt
authors.forename: Sven
authors.initials: S
authors.lastname: Brandau
authors.forename: Stefan
authors.initials: S
authors.lastname: Mattheis
authors.forename: Kathrin
authors.initials: K
authors.lastname: Scheckenbach
authors.forename: Oliviera J
authors.initials: OJ
authors.lastname: Finn
authors.forename: Theresa L
authors.initials: TL
authors.lastname: Whiteside
authors.forename: Enikö
authors.initials: E
authors.lastname: Sonkoly
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D056744:Cyclin B1 / Q000378:metabolism*; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C534699:CCNB1 protein, human; D056744:Cyclin B1; D016159:Tumor Suppressor Protein p53