title: Highlighting anatomical sub-structures in rat brain tissue using lipid imaging.
abstract: Cell membranes are made up of a mixture of glycerolipids, sphingolipids, gangliosides and cholesterol. Lipids play important roles in a cell's life. However many of their functions have still to be discovered. In the present work, we describe an efficient, easy and rapid methodology to accurately localize phosphatidylcholines and sphingomyelins from a single coronal rat brain section in the cerebrum area. Matrix assisted laser desorption/ionization (MALDI) mass spectrometry was used to profile and image lipids. The best resolved structure was 25-50 μm in the hippocampus.
authors.affiliation: Cellular Neurobiology, NIDA IRP, NIH, 333 Cassell Drive, Room 1120, Baltimore, MD, 21224, USA.
authors.forename: Alice M
authors.initials: AM
authors.lastname: Delvolve
authors.forename: Benoit
authors.initials: B
authors.lastname: Colsch
authors.forename: Amina S
authors.initials: AS
authors.lastname: Woods