title: Comparative assessment of essential and toxic metals in the blood of rheumatoid arthritis patients and healthy subjects.
abstract: The present study deals with the comparative evaluation of essential and toxic metals in rheumatoid arthritis and healthy donors. Blood samples collected from rheumatoid arthritis patients and healthy subjects were analysed for selected essential and toxic metals (Ca, Mg, Fe, Zn, Cu, Co, Mn, Cr, Cd and Pb). The samples were digested in nitric acid and perchloric acid mixture, followed by quantification of the metals using atomic absorption spectrometry. Mean levels of Ca, Mg, Fe and Zn were significantly higher in the blood of healthy donors; however, elevated levels of Cd, Co, Cr, Cu and Pb were observed in blood of the patients. The correlation coefficients among the selected metals in the blood of arthritis patients were significantly different compared with the healthy counterparts. Multivariate cluster analysis revealed mutual apportionment of the essential and toxic metals in blood of the patients, whereas, in controls, the essential and toxic metals revealed diverse apportionment. Variations in the metal levels with gender, residence and smoking habits were also evaluated in both donor groups. Relative distribution, correlation and apportionment of the essential and toxic metals in the blood of the patients were significantly different than of controls.
authors.affiliation: Department of Chemistry, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
authors.forename: G M Mujtba
authors.initials: GM
authors.lastname: Hashmi
authors.forename: Munir H
authors.initials: MH
authors.lastname: Shah
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008670:Metals / Q000032:analysis* / Q000633:toxicity; D008875:Middle Aged; D015999:Multivariate Analysis; D014131:Trace Elements / Q000032:analysis* / Q000097:blood* / Q000633:toxicity
chemical_list: D008670:Metals; D014131:Trace Elements