title: Calcium phosphate bioceramics fabricated from extracted human teeth for tooth tissue engineering.
abstract: Bioceramic tooth powders were prepared via heat treatment of extracted human teeth using sintering temperatures between 600°C and 1200°C, and their properties were investigated for potential tooth tissue engineering. The sintered human tooth powders were characterized using thermal analysis (thermogravimetric analysis (TG) and differential thermal analysis (DTA)), field emission scanning electron microscopy, X-ray diffraction, energy dispersive X-ray spectroscopy, and Fourier transformed infrared (FTIR) spectroscopy. Additionally, the phase constitutions and chemical homogeneities of the composite samples were examined using a quantitative chemical analysis with inductively coupled plasma spectroscopy. The results revealed that the annealing process produced useful hydroxyapatite-based bioceramic biomaterials when annealed above 1000°C. The FTIR spectra and the TG/DTA thermograms of the tooth powders indicated the presence of organic compounds, which were completely removed after annealing at temperatures above 1000°C. The tooth powders annealed between 1000°C and 1200°C had good characteristics as bioceramic biomaterials. Furthermore, the biocompatibility of each tooth powder was evaluated using in vitro and in vivo techniques; our results indicate that the prepared human tooth powders have great potential for tooth tissue engineering applications.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Ki-Taek
authors.initials: KT
authors.lastname: Lim
authors.forename: Je Duck
authors.initials: JD
authors.lastname: Suh
authors.forename: Jangho
authors.initials: J
authors.lastname: Kim
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Choung
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D008297:Male; D008422:Materials Testing; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011208:Powders / Q000737:chemistry; D012038:Regeneration; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013234:Stem Cells / Q000166:cytology; D013696:Temperature; D013818:Thermogravimetry / Q000379:methods; D023822:Tissue Engineering / Q000379:methods*; D014070:Tooth / Q000502:physiology*; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011208:Powders; C020243:calcium phosphate