title: Characterization of calcium channel binding.
abstract: Voltage-dependent calcium channels are expressed in a variety of tissues including heart, muscles and brain. Saturation binding of a radioligand to the calcium channel is commonly used to characterize the expression level of the channel protein. Compound competition binding assay is a conventional screening method to determine the affinity of unlabeled compounds for the channel protein. This unit provides detailed experimental methods for two types of radioligand binding assays using [³H]PN200-100 and [¹²⁵I](conotoxin MVIIA. Voltage-dependent calcium channels are expressed in a variety of tissues including heart, muscles and brain.
authors.affiliation: Merck and Company, North Wales, Pennsylvania, USA.
authors.forename: W
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding / Q000502:physiology; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels