title: RLIM interacts with Smurf2 and promotes TGF-β induced U2OS cell migration.
abstract: TGF-β (transforming growth factor-β), a pleiotropic cytokine that regulates diverse cellular processes, has been suggested to play critical roles in cell proliferation, migration, and carcinogenesis. Here we found a novel E3 ubiquitin ligase RLIM which can directly bind to Smurf2, enhancing TGF-β responsiveness in osteosarcoma U2OS cells. We constructed a U2OS cell line stably over-expressing RLIM and demonstrated that RLIM promoted TGF-β-driven migration of U2OS cells as tested by wound healing assay. Our results indicated that RLIM is an important positive regulator in TGF-β signaling pathway and cell migration.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Huang
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.forename: Xianmei
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Yan
authors.forename: Chenji
authors.initials: C
authors.lastname: Wang
authors.forename: Sirui
authors.initials: S
authors.lastname: Jiang
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D047369:Chromatin Immunoprecipitation; D006801:Humans; D016212:Transforming Growth Factor beta / Q000494:pharmacology / Q000502:physiology*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*
chemical_list: D016212:Transforming Growth Factor beta; C471641:SMURF2 protein, human; C492370:RLIM protein, human; D044767:Ubiquitin-Protein Ligases