title: Glycan microarrays.
abstract: Glycan microarrays are presentations of multiple glycans or glycoconjugates printed on a single slide for screening with glycan-binding proteins (GBPs), which include lectins, antibodies, bacteria, and viruses. Glycans derivatized with functional groups can be immobilized onto appropriately activated glass slides to generate glycan microarrays where each glycan is printed at similar concentrations. Here we describe a method for fluorescently and functionally derivatizing free reducing glycans, printing microarrays, and interrogating the microarrays with GBPs.
authors.affiliation: Department of Biochemistry and The Glycomics Center, Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Xuezheng
authors.initials: X
authors.lastname: Song
authors.forename: Jamie
authors.initials: J
authors.lastname: Heimburg-Molinaro
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
authors.forename: Richard D
authors.initials: RD
authors.lastname: Cummings
mesh_terms: D001549:Benzamides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011327:Printing; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: C539333:2-amino-N-(2-aminoethyl)benzamide; D001549:Benzamides; D005456:Fluorescent Dyes; D011134:Polysaccharides; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins