title: Quantum dot-based immunochromatography test strip for rapid, quantitative and sensitive detection of alpha fetoprotein.
abstract: Rapid, quantitative detection of tumor markers with high sensitivity and specificity is critical to clinical diagnosis and treatment of cancer. We describe here a novel portable fluorescent biosensor that integrates quantum dot (QD) with an immunochromatography test strip (ICTS) and a home-made test strip reader for detection of tumor markers in human serum. Alpha fetoprotein (AFP), which is valuable for diagnosis of primary hepatic carcinoma, is used as a model tumor marker to demonstrate the performance of the proposed immunosensor. The principle of this sensor is on the basis of a sandwich immunoreaction that was performed on an ICTS. The fluorescence intensity of captured QD labels on the test line and control line served as signals was determined by the home-made test strip reader. The strong luminescence and robust photostability of QDs combined with the promising advantages of an ICTS and sensitive detection with the test strip reader result in good performance. Under optimal conditions, this biosensor is capable of detecting as low as 1 ng/mL AFP standard analyte in 10 min with only 50 μL sample volume. Furthermore, 1000 clinical human serum samples were tested by both the QD-based ICTS and a commercial electrochemiluminescence immunoassay AFP kit simultaneously to estimate the sensitivity, specificity and concordance of the assays. Results showed high consistency except for 24 false positive cases (false positive rate 3.92%) and 17 false negative cases (false negative rate 4.38%); the error rate was 4.10% in all. This demonstrates that the QD-based ICTS is capable of rapid, sensitive, and quantitative detection of AFP and shows a great promise for point-of-care testing of other tumor markers.
authors.affiliation: Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Yang
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Gong
authors.forename: Tao
authors.initials: T
authors.lastname: Song
authors.forename: Jiumin
authors.initials: J
authors.lastname: Yang
authors.forename: Shengjiang
authors.initials: S
authors.lastname: Zhu
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Cui
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Li
authors.forename: Bingbo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation; D002846:Chromatography, Affinity / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D009363:Neoplasm Proteins / Q000032:analysis*; D045663:Quantum Dots; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D011934:Reagent Strips; D000509:alpha-Fetoproteins