title: Triterpenoid constituents from the roots of Paeonia rockii ssp. rockii.
abstract: An investigation of a chloroform-soluble extract from the roots of Paeonia rockii ssp. rockii yielded three new noroleanane triterpenoids (1-3) together with 19 known compounds. Their structures were established by analysis of the spectroscopic data. The effects of this chloroform-soluble extract and its major constituents on cell proliferation and apoptosis of a panel of human cancer cell lines (melanoma M-14, colon cancer HT-29, breast cancer MCF-7) were evaluated by the MTT bioassay and propidium iodide staining, respectively, in comparison with normal human embryonic kidney cells (HEK-293). Two of the triterpenoids, betulinic acid (4) and oleanolic acid (5), and the crude extract were cytotoxic and induced apoptosis selectively in the M-14 melanoma cell line. This effect was reversed by the caspase-inhibitor z-VAD-fmk, suggesting that such action is mediated by caspase-3 activation.
authors.affiliation: Dipartimento di Scienze Farmaceutiche e Biomediche, Università di Salerno, Via Ponte Don Melillo, 84084, Fisciano (SA), Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Mencherini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Picerno
authors.forename: Michela
authors.initials: M
authors.lastname: Festa
authors.forename: Paola
authors.initials: P
authors.lastname: Russo
authors.forename: Anna
authors.initials: A
authors.lastname: Capasso
authors.forename: Rita
authors.initials: R
authors.lastname: Aquino
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds / Q000494:pharmacology; D061945:Caspase Inhibitors; D004354:Drug Screening Assays, Antitumor; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D007558:Italy; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029596:Paeonia / Q000737:chemistry*; D053978:Pentacyclic Triterpenes; D010851:Picrates / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000094062:Betulinic Acid
chemical_list: C568750:23-deoxypaeonenoide A; C568751:3beta,23-dihydroxy-30-nor-olean-12,20-dien-28-oic acid; C568752:3beta,4beta,23-trihydroxy-24-nor-olean-12-en-28-oic acid; D000590:Amino Acid Chloromethyl Ketones; D000972:Antineoplastic Agents, Phytogenic; D001713:Biphenyl Compounds; D061945:Caspase Inhibitors; D053978:Pentacyclic Triterpenes; D010851:Picrates; D014315:Triterpenes; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D009828:Oleanolic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D000094062:Betulinic Acid