title: Screening of differentially expressed genes and predominant expression of β variable region of T cell receptor in peripheral T cells of psoriatic patients.
abstract: Psoriasis is a skin disease featuring epithelial cell hyper-proliferation and T cell infiltration. Abnormal T cell immune responses play an important role in psoriatic pathogenesis. To screen differentially expressed genes in T cells of patients with plaque psoriasis, analyze the predominant expression of the β variable region of T cell receptors and discuss the role of T cells in the pathogenesis of psoriasis. High throughput RNA sequencing and Real-time PCR were used.
  
  
  RESULTS
  A total of 907 genes were differentially expressed in peripheral T cells of patients with psoriasis. Among them, 695 genes were mapped to the Gene Ontology database, 14 gene terms were significantly enriched, and 418 genes were involved in signaling pathways such as apoptosis, B cell receptor signaling and T cell receptor signaling. TRBV2, TRBV5-7, TRBV6-6/6-9, TRBV12, TRBV24 and TRBV29 were significantly up-regulated in psoriatic patients compared to healthy subjects, among which, TRBV6-6/6-9, TRBV12 and TRBV29 are predominantly expressed in psoriatic patients. Many genes were differentially expressed in T cells of psoriatic patients, especially the TRBV gene family, which were predominantly expressed in T cells and might play an important role in abnormal immune responses of T cells in psoriatic patients.
authors.affiliation: Department of Dermatology, Taiyuan Center Hospital, Shanxi Province, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yin
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.forename: Junqin
authors.initials: J
authors.lastname: Li
authors.forename: Hongzhou
authors.initials: H
authors.lastname: Cui
authors.forename: Ruixia
authors.initials: R
authors.lastname: Hou
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Dong
authors.forename: Honggang
authors.initials: H
authors.lastname: Liang
authors.forename: Kaiming
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D019674:Genes, T-Cell Receptor beta / Q000502:physiology*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000235:genetics* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*; D055815:Young Adult
chemical_list: D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell