title: Development of an assay of seven biochemical items, HbA1c, and hematocrit using a small amount of blood collected from the fingertip.
abstract: BACKGROUND
  Lifestyle-related diseases in Japan account for 30% of the entire medical expenditure of the country and cause 60% of all deaths. For the prevention of lifestyle-related diseases, medical examination by laboratory tests on metabolic syndrome is important.
  
  
  METHODS
  To undertake examination by collection of blood from a fingertip, we developed the "Well Kit". About 65 μl of blood collected from a fingertip was diluted with buffer solution, which contained two internal standard materials. The kit also separated corpuscles and diluted plasma with a special filter. It measured the obtained diluted plasma using the JCA-BM2250.
  
  
  RESULTS
  This measurement system was evaluated for the quantitative analysis of 8 items. The uncertainties of tested items of this measurement system were 1.7% to 6.4%. The coefficients of correlation of all tested items between this measurement value and the venous plasma sample value were 0.876-0.991, and hematocrit was 0.958.
  
  
  CONCLUSIONS
  This system for testing blood collected from a fingertip is simple to use and can be applied in testing for metabolic syndrome. In addition, this testing system is useful in the medical examination of the personal healthcare and inhabitants.
authors.affiliation: Physical Screening, Inc., Tokyo, Japan. sugimoto@pcl-g.co.jp
authors.forename: Sugimoto
authors.initials: S
authors.lastname: Shinya
authors.forename: Akimoto
authors.initials: A
authors.lastname: Masaru
authors.forename: Hayakawa
authors.initials: H
authors.lastname: Akira
authors.forename: Hokazono
authors.initials: H
authors.lastname: Eisaku
authors.forename: Osawa
authors.initials: O
authors.lastname: Susumu
mesh_terms: D002138:Calibration; D002794:Choline / Q000097:blood*; D019963:Clinical Chemistry Tests; D005385:Fingers; D006442:Glycated Hemoglobin / Q000032:analysis*; D006400:Hematocrit; D006801:Humans; D014439:Tyramine / Q000097:blood*; D035501:Uncertainty
chemical_list: D002794:Choline; D006442:Glycated Hemoglobin; D014439:Tyramine