title: Quasispecies spatial models for RNA viruses with different replication modes and infection strategies.
abstract: Empirical observations and theoretical studies suggest that viruses may use different replication strategies to amplify their genomes, which impact the dynamics of mutation accumulation in viral populations and therefore, their fitness and virulence. Similarly, during natural infections, viruses replicate and infect cells that are rarely in suspension but spatially organized. Surprisingly, most quasispecies models of virus replication have ignored these two phenomena. In order to study these two viral characteristics, we have developed stochastic cellular automata models that simulate two different modes of replication (geometric vs stamping machine) for quasispecies replicating and spreading on a two-dimensional space. Furthermore, we explored these two replication models considering epistatic fitness landscapes (antagonistic vs synergistic) and different scenarios for cell-to-cell spread, one with free superinfection and another with superinfection inhibition. We found that the master sequences for populations replicating geometrically and with antagonistic fitness effects vanished at low critical mutation rates. By contrast, the highest critical mutation rate was observed for populations replicating geometrically but with a synergistic fitness landscape. Our simulations also showed that for stamping machine replication and antagonistic epistasis, a combination that appears to be common among plant viruses, populations further increased their robustness by inhibiting superinfection. We have also shown that the mode of replication strongly influenced the linkage between viral loci, which rapidly reached linkage equilibrium at increasing mutations for geometric replication. We also found that the strategy that minimized the time required to spread over the whole space was the stamping machine with antagonistic epistasis among mutations. Finally, our simulations revealed that the multiplicity of infection fluctuated but generically increased along time.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, València, Spain. josep.sardanes@upf.edu
authors.forename: Josep
authors.initials: J
authors.lastname: Sardanyés
authors.forename: Santiago F
authors.initials: SF
authors.lastname: Elena
mesh_terms: D001483:Base Sequence; D003198:Computer Simulation; D004843:Epistasis, Genetic; D016679:Genome, Viral / Q000235:genetics; D008957:Models, Genetic; D059645:Mutation Rate; D012327:RNA Virus Infections / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D013997:Time Factors; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*