title: Antiinflammatory effect of low-level laser therapy on Staphylococcus epidermidis endophthalmitis in rabbits.
abstract: A rabbit model of endophthalmitis was established to evaluate the antiinflammatory effect of low-level laser therapy (LLLT) as an adjunct to treatment for Staphylococcus epidermidis endophthalmitis. Rabbits were randomly divided into three groups to receive intravitreal injections into their left eye: group A received 0.5 mg vancomycin (100 μl), group B received 0.5 mg vancomycin + 0.2 mg dexamethasone (100 μl), and group C received 0.5 mg vancomycin (100 μl) and continuous wave semiconductor laser irradiation (10 mW, λ = 632 nm) focused on the pupil. Slit lamp examination and B-mode ultrasonography were conducted to evaluate the symptoms of endophthalmitis. Polymorphonuclear cells and tumour necrosis factor alpha (TNF-α) in aqueous fluid were measured at 0 h, and 1, 2, 3, 7 and 15 days. A histology test was conducted at 15 days. B-mode ultrasonography and histology revealed that groups B and C had less inflammation than group A at 15 days. Groups B and C had fewer polymorphonuclear cells and lower levels of TNF-α in aqueous fluid than group A at 2, 3 and 7 days (P < 0.05). There was no significant difference between groups B and C (P > 0.05). There was no significant difference between groups A, B and C at 15 days (P > 0.05). As an adjunct to vancomycin therapy to treat S. epidermidis endophthalmitis, LLLT has an antiinflammatory effect similar to that of dexamethasone.
authors.affiliation: Tianjin Medical University Eye Centre, Tianjin, 300070, China.
authors.forename: Wen-jiang
authors.initials: WJ
authors.lastname: Ma
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Li
authors.forename: Ying-xin
authors.initials: YX
authors.lastname: Li
authors.forename: Zhi-xiao
authors.initials: ZX
authors.lastname: Xue
authors.forename: Hui-juan
authors.initials: HJ
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Ma
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy; D003907:Dexamethasone / Q000008:administration & dosage; D009877:Endophthalmitis / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D011817:Rabbits; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy*; D013212:Staphylococcus epidermidis; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014463:Ultrasonography; D014640:Vancomycin / Q000008:administration & dosage
chemical_list: D014409:Tumor Necrosis Factor-alpha; D014640:Vancomycin; D003907:Dexamethasone