title: [Effects of phytohormones on hairy root growth and tanshinone biosynthesis of Salvia miltiorrhiza].
abstract: OBJECTIVE
  To study the effects of different phytohormones on the growth of hairy root and biosynthesis of tanshinone II A in Salvia miltiorrhiza.
  
  
  METHODS
  Salvia miltiorrhiza hairy root was induced by organizational culture method. Tanshinone was extracted with ultrasonic method and detected by high performance liquid chromatography (HPLC).
  
  
  RESULTS
  The phytohormone combination of GA3 and 6-BA at different concentrations adding to 1/2 MS medium had obviously inhibitory effect on the hairy root growth of Salvia miltiorrhiza. And the highest inhibition rate was up to 100% (hairy roots were death). However, the growth of hairy roots could be obviously promoted by the combination of KT (1.0 mg/L) and IBA (1.0 mg/L), increasing 3. 251 times than that of the control. Total tanshinone II A content in hairy roots bodies was the highest under the combination of 0.2 mg/L NAA and 3.0 mg/L 6-BA. 6-BA of 2.0 mg/L played a significant role in promoting tanshinone II A biosynthesis, and the tanshinone II A concentration was 3.012 times higher than that of the control.
  
  
  CONCLUSIONS
  Different phytohormones and its combination adding to 1/2 MS medium has significant influence on the hairy root growth and the content of tanshinone II A in Salvia miltiorrhiza.
authors.affiliation: School of Life Science, Anhui Agricultural University, Hefei 230036, China. pipi8421@163.com
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Fang
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Wang
authors.forename: Zhi-Fen
authors.initials: ZF
authors.lastname: Wang
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Shan
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Ni
authors.forename: Yu-Qiong
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D045784:Abietanes / Q000096:biosynthesis*; D019149:Bioreactors; D003470:Culture Media; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology*; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D010946:Plants, Medicinal / Q000254:growth & development* / Q000378:metabolism; D027561:Salvia miltiorrhiza / Q000254:growth & development* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D045784:Abietanes; D003470:Culture Media; D010937:Plant Growth Regulators; C021751:tanshinone