title: Gene expression in swine  granulosa cells and ovarian tissue during the estrous cycle.
abstract: The  components of the insulin-like growth factor (IGF) system appear to be involved  in regulation of ovarian follicular growth and atresia in the pig. We  investigated the expression pattern of mRNAs for IGF1 (IGF1), its  binding proteins (IGFBP1, IGFBP2, IGFBP3, and IGFBP5),  and epidermal growth factor in swine follicle cells and ovarian tissue  throughout the estrous cycle using the real-time quantitative PCR technique.  The results of gene expression were analyzed using linear regression with gene  expression as a dependent variable and days of estrous cycle as an independent  variable. Additionally, an analysis was made of the correlation of expression  levels with plasma concentration of follicle-stimulating hormone, luteinizing  hormone, estradiol-17β,  progesterone, and prolactin. Expression of mRNA of all of these genes was  detected in granulosa cells and ovarian tissue. IGFBP3 mRNA showed a  quadratic expression pattern (P ≤ 0.001) and was significantly and positively  correlated with progesterone (r = 0.81; P ≤ 0.01) but negatively correlated  with prolactin (r = -0.596; P ≤ 0.05). Expression of the other genes was  unaffected by the stage of the estrous cycle. Real-time quantitative PCR  effectively detected all transcripts, including the very low levels of IGFBP1 transcripts, and could be used for studies of follicle dynamics.
authors.affiliation: Departamento de Zootecnia, Universidade Federal de Viçosa, Viçosa, MG, Brasil.
authors.forename: P V
authors.initials: PV
authors.lastname: Silva
authors.forename: S E F
authors.initials: SE
authors.lastname: Guimarães
authors.forename: J D
authors.initials: JD
authors.lastname: Guimarães
authors.forename: J B
authors.initials: JB
authors.lastname: Neto
authors.forename: P S
authors.initials: PS
authors.lastname: Lopes
authors.forename: C S
authors.initials: CS
authors.lastname: do Nascimento
authors.forename: C F
authors.initials: CF
authors.lastname: de Campos
authors.forename: M M C A
authors.initials: MM
authors.lastname: Weller
authors.forename: M E
authors.initials: ME
authors.lastname: Botelho
authors.forename: V R
authors.initials: VR
authors.lastname: Faria
mesh_terms: D000818:Animals; D004815:Epidermal Growth Factor / Q000096:biosynthesis / Q000235:genetics; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000235:genetics*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D015870:Gene Expression; D006107:Granulosa Cells / Q000166:cytology / Q000502:physiology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000096:biosynthesis / Q000235:genetics; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000096:biosynthesis / Q000235:genetics; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000096:biosynthesis / Q000235:genetics; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000096:biosynthesis / Q000235:genetics; D007986:Luteinizing Hormone / Q000097:blood; D006080:Ovarian Follicle / Q000254:growth & development; D010053:Ovary / Q000166:cytology* / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013002:Somatomedins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics
chemical_list: D018970:Insulin-Like Growth Factor Binding Protein 1; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D018975:Insulin-Like Growth Factor Binding Protein 5; D012333:RNA, Messenger; D013002:Somatomedins; D011374:Progesterone; D004958:Estradiol; D004815:Epidermal Growth Factor; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone