title: Cadherin-integrated liposomes with potential application in a drug delivery system.
abstract: N-cadherin (CDH2) proteins were reconstituted with liposomes using a baculovirus expression-liposome fusion method. CDH2 budded viruses were fused with giant liposomes containing dioleoylphophogycerol/dioleoylphosphatidylcholine (DOPG/DOPC) at pH 4.5 and the localization of CDH2 on the liposome membrane was observed by confocal laser scanning microscopy. CDH2 liposomes showed Ca(2+)-dependent association. CDH2-mediated association/dissociation in CDH2 liposomes was specific to Ca(2+) and reversible. CDH2-expressing LN-229 cells (human glioblastoma cell) adhered to CDH2 liposomes and small CDH2 liposomes (diameter approximately 150 nm), in particular, were internalized by endocytosis and partly escaped endosomes. Cadherin-containing liposomes show high potential as a new cell-specific proteoliposome. The baculovirus expression-liposome fusion method is useful as a new enabling technology for biomedical applications of functional proteoliposomes.
authors.affiliation: Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Kamiya
authors.forename: Kanta
authors.initials: K
authors.lastname: Tsumoto
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D000268:Adhesiveness; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D002450:Cell Communication; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism; D008954:Models, Biological; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D015820:Cadherins; D008081:Liposomes; D011994:Recombinant Proteins