title: Reinforced nanohydroxyapatite/polyamide66 scaffolds by chitosan coating for bone tissue engineering.
abstract: High porosity of scaffold is always accompanied by poor mechanical property; the aim of this study was to enhance the strength and modulus of the highly porous scaffold of nanohydroxyapatite/polyamide66 (n-HA/PA66) by coating chitosan (CS) and to investigate the effect of CS content on the scaffold physical properties and cytological properties. The results show that CS coating can reinforce the scaffold effectively. The compress modulus and strength of the CS coated n-HA/PA66 scaffolds are improved to 32.71 and 2.38 MPa, respectively, being about six times and five times of those of the uncoated scaffolds. Meanwhile, the scaffolds still exhibit a highly interconnected porous structure and the porosity is approximate about 78%, slightly lower than the value (84%) of uncoated scaffold. The cytological properties of scaffolds were also studied in vitro by cocultured with osteoblast-like MG63 cells. The cytological experiments demonstrate that the reinforced scaffolds display favorable cytocompatibility and have no significant difference with the uncoated n-HA/PA66 scaffolds. The CS reinforced n-HA/PA66 scaffolds can meet the basic mechanical requirement of bone tissue engineering scaffold, presenting a potential for biomedical application in bone reconstruction and repair.
authors.affiliation: Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zuo
authors.forename: Qin
authors.initials: Q
authors.lastname: Zou
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.forename: Shibo
authors.initials: S
authors.lastname: Gao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Haohuai
authors.initials: H
authors.lastname: Liu
authors.forename: Yubao
authors.initials: Y
authors.lastname: Li
mesh_terms: D001861:Bone Regeneration; D001842:Bone and Bones; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D009757:Nylons; D048271:Chitosan; D017886:Durapatite