title: Preliminary proteomic analysis of human serum from patients with laryngeal carcinoma.
abstract: To better evaluate the serum protein alterations in patients with laryngeal carcinoma during surgical treatment process, a comparative proteomic analysis of human serum from patients with laryngeal carcinoma between pre- and post-operation group was performed using two-dimensional gel electrophoresis, in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database searching. Statistical analysis indicated that 16 gel spots corresponding to 12 proteins altered their expression significantly between the two groups of patients with laryngeal carcinoma. Of these 12 proteins, 6 proteins were up-regulated in the pre-operation group. The subsequent Western blot confirmed the results of proteome analysis. Measurement of MDA and SOD levels in serum combining with bioinformatics analysis indicated the potential roles of the oxidant stress and immune response as target in monitoring and treating laryngeal carcinoma. The study provides new insight into the laryngeal carcinoma development and treatment, and the identified proteins are warranted to be further studied.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Yantai Yu-huang-ding Hospital, No. 20, Yu-huang-ding Dong Road, Yantai, 264000, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Xicheng
authors.initials: X
authors.lastname: Song
authors.forename: Xuexia
authors.initials: X
authors.lastname: Liu
authors.forename: Qingquan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiumei
authors.initials: X
authors.lastname: Chen
authors.forename: Chenglu
authors.initials: C
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery; D007825:Laryngectomy; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D009367:Neoplasm Staging; D018384:Oxidative Stress / Q000502:physiology; D040901:Proteomics / Q000379:methods*; D013482:Superoxide Dismutase / Q000097:blood; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D008315:Malondialdehyde; D013482:Superoxide Dismutase