title: Anti-tissue transglutaminase antibodies (IgA and IgG) in both Crohn´s disease and autoimmune diabetes.
abstract: OBJECTIVE
  a strong association has been observed between celiac disease, generally its silent clinical form, and autoimmune disorders. A potential correlation with inflammatory bowel disease has also been suggested. Anti-tissue transglutaminase antibodies have been detected in Crohn´s disease. We investigated the prevalence of celiac disease in patients with autoimmune diabetes and in Crohn´s disease patients and also evaluated the correlation between anti-transglutaminase antibody positivity and the clinical status of these diseases.
  
  
  METHODS
  anti-tissue transglutaminase and anti-endomysium antibodies were assessed by enzyme-linked immunosorbent assay and indirect immunofluorescence, respectively. Upper digestive endoscopy and duodenal biopsy were indicated for cases with positive serology.
  
  
  RESULTS
  anti-transglutaminase antibodies were detected in five diabetic patients (prevalence of 11.1%), only one serum sample was positive for IgG isotypes. Nine of thirty-three patients with Crohn´s disease had low positive levels for IgA anti-transglutaminase. Antiendomysium antibodies were detected only in celiac patients. Celiac disease was confirmed in all diabetic patients submitted to duodenal biopsies who presented both anti-transglutaminase and anti-endomisyum antibodies positivity. In Crohn´s disease, its clinical status and the diagnosis of celiac disease were not associated with positiveanti-transglutaminase result.
  
  
  CONCLUSIONS
  the prevalence of celiac disease was high in diabetic patients. Anti-tissue transglutaminase antibodies were sensitive and specific markers of celiac disease in this diabetic group, while these antibodies were of limited value for celiac disease screening in patients with Crohn´s disease.
authors.affiliation: Department of Internal Medicine, Division of Gastroenterology, Federal University of São Paulo, São Paulo, Brazil. virginiacabral@terra.com.br
authors.forename: Virgínia Lúcia
authors.initials: VL
authors.lastname: Ribeiro-Cabral
authors.forename: Francy Reis
authors.initials: FR
authors.lastname: da-Silva-Patrício
authors.forename: Orlando
authors.initials: O
authors.lastname: Ambrogini-Junior
authors.forename: Sender
authors.initials: S
authors.lastname: Jankiel-Miszputen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000150:complications* / Q000276:immunology / Q000473:pathology; D003424:Crohn Disease / Q000150:complications* / Q000276:immunology / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000150:complications* / Q000276:immunology / Q000473:pathology; D004384:Duodenoscopy; D004386:Duodenum / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011503:Transglutaminases / Q000276:immunology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011503:Transglutaminases