title: In vitro lipopolysaccharide treatment alters regulatory T cell properties in chickens.
abstract: The objective of this study was to identify the effect of in vitro lipopolysaccharide (LPS) treatment on regulatory T cells (Tregs) from chickens. Tregs had approximately 30-fold higher TLR 2-type 2 and six-fold higher TLR 4 mRNA content than CD4+CD25- cells. Tregs were treated with either 0 or 1 μg/ml LPS for 0, 2, and 4d. LPS treatment increased the IL-2 mRNA amount in Tregs at 2 and 4d post-LPS treatment. LPS treatment increased the IL-10 mRNA amount in Tregs at 4d post-LPS treatment. The total live cell numbers were approximately two-fold higher at 2d and three-fold higher at 4d in the 1 μg/ml LPS-treated groups than in the 0 μg LPS-treated controls. LPS treatment abrogated suppressive properties of Tregs at 2d post-LPS treatment. At 4d post-LPS treatment, Tregs became supersuppressive. In conclusion, chicken Tregs are differentially activated to facilitate immune response during the early stage of inflammation and to facilitate immune suppression at a later stage of inflammation.
authors.affiliation: Department of Animal Sciences, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States.
authors.forename: Revathi
authors.initials: R
authors.lastname: Shanmugasundaram
authors.forename: Ramesh K
authors.initials: RK
authors.lastname: Selvaraj
mesh_terms: D000818:Animals; D002645:Chickens / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects; D016753:Interleukin-10 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count / Q000662:veterinary; D012333:RNA, Messenger / Q000187:drug effects; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D007376:Interleukin-2; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D016753:Interleukin-10