title: Immunological effects of taxol and adryamicin in breast cancer patients.
abstract: Antineoplastic chemotherapy still consists in the major first-line therapeutics against cancer. Several reports have described the immunomodulatory effects of these drugs based on in vitro treatment, but no previous data are known about these effects in patients and its association with immunological-mediated toxicity. In this study, we first characterize the immunological profile of advanced breast cancer patients treated with doxorubicin and paclitaxel protocols, immediately after chemotherapy infusion. Our findings included an immediate plasmatic reduction in IL-1, IL-10, and TNF-α levels in doxorubicin-treated patients, as well as high levels of IL-10 in paclitaxel patients. Further, it was demonstrated that both drugs led to leukocytes oxidative burst impairment. In vitro analysis was performed exposing healthy blood to both chemotherapics in the same concentration and time of exposition of patients, resulting in low IL-10 and high IL-1β in doxorubicin exposition, as low TNF-α and high IL-1 in paclitaxel treatment. Nitric oxide levels were not altered in both in vivo and in vitro treatments. In conclusion, our data revealed for the first time that the immediate effects of chemotherapy could be mediated by cytokines signaling in patients and that the results observed in patients could be a resultant of host immune cells activation.
authors.affiliation: Laboratory of Physiopathology and Free Radicals, Department of General Pathology-Center of Biological Science, State University of Londrina, Londrina, 86051-990, Brazil. carolpanis@sercomtel.com.br
authors.forename: C
authors.initials: C
authors.lastname: Panis
authors.forename: L G T
authors.initials: LG
authors.lastname: Lemos
authors.forename: V J
authors.initials: VJ
authors.lastname: Victorino
authors.forename: A C S A
authors.initials: AC
authors.lastname: Herrera
authors.forename: F C
authors.initials: FC
authors.lastname: Campos
authors.forename: A N
authors.initials: AN
authors.lastname: Colado Simão
authors.forename: P
authors.initials: P
authors.lastname: Pinge-Filho
authors.forename: A L
authors.initials: AL
authors.lastname: Cecchini
authors.forename: R
authors.initials: R
authors.lastname: Cecchini
mesh_terms: D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002277:Carcinoma / Q000175:diagnosis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000097:blood; D004317:Doxorubicin / Q000008:administration & dosage* / Q000009:adverse effects; D005260:Female; D006801:Humans; D056747:Immunomodulation; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017239:Paclitaxel / Q000008:administration & dosage* / Q000009:adverse effects
chemical_list: D016207:Cytokines; D009569:Nitric Oxide; D004317:Doxorubicin; D017239:Paclitaxel