title: [SUMO-2/3 can covalently bind to progesterone receptor B to regulate its transcriptional activity].
abstract: OBJECTIVE
  To investigate whether progesterone receptor B (PRB) can be sumoylated by SUMO-2/3 and the effect of sumoylation on PRB transcriptional activity.
  
  
  METHODS
  SUMO-2/3 cDNA was amplified from MCF-7 cDNA and cloned into the eukaryotic expression vector pcDNA3-FLAG. The plasmid pXJ40-myc-PRB was cotransfected with pcDNA3FLAG-SUMO2, pcDNA3FLAG-SUMO3 or the mock control into 293T cells, and PRB sumoylation was detected by immunoprecipitation and Western blotting. The effect of PRB sumoylation on its transcriptional activity was determined using reporter luciferase assay.
  
  
  RESULTS
  pcDNA3FLAG-SUMO2 and pcDNA3FLAG-SUMO3 vectors were successfully constructed. SUMO-2/3 could bind covalently to PRB and increase its transcriptional dependent on the presence of progesterone.
  
  
  CONCLUSIONS
  PRB can be sumoylated by SUMO-2/3 and its function is regulated by this modification.
authors.affiliation: Department of Endocrinology, General Hospital of PLA, Beijing 100853, China. bzy629@yahoo.cn
authors.forename: Bai-yu
authors.initials: BY
authors.lastname: Han
authors.forename: Fa-ceng
authors.initials: FC
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Xu
authors.forename: Kai
authors.initials: K
authors.lastname: Jiang
authors.forename: Jie
authors.initials: J
authors.lastname: Fu
authors.forename: Yong-jian
authors.initials: YJ
authors.lastname: Han
authors.forename: Zhao-hui
authors.initials: ZH
authors.lastname: Lv
authors.forename: Jing-tao
authors.initials: JT
authors.lastname: Dou
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Qi-nong
authors.initials: QN
authors.lastname: Ye
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011980:Receptors, Progesterone / Q000235:genetics* / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D054875:Ubiquitination; D014452:Ubiquitins / Q000235:genetics* / Q000378:metabolism
chemical_list: D011980:Receptors, Progesterone; C117909:SUMO2 protein, human; C492189:SUMO3 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014452:Ubiquitins; C097314:progesterone receptor B