title: Distinct In Vitro Myelopoiesis is Dependent on the Self-Renewal of Hematopoietic Progenitors.
abstract: Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic-like antigen-presenting cell type (L-DC). These progenitors are also maintained in both long-term spleen cultures and co-cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L-DC progenitors by testing their capacity to colonize STX3 and produce L-DC. E14.5 FL from wild-type C57BL/6J mice was found to colonize STX3 and produce L-DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short-term production of low numbers of L-DC between 7 and 14 days of co-culture. The transient and weak production of L-DC by FL from Plastic E14.5 mice maps to the loss of self-renewal capacity amongst HSC. L-DC progenitors are, therefore, closely aligned with a subset of self-renewing HSC/HPC in FL.
authors.affiliation: Immunology and Stem Cell Laboratory, Research School of Biology, Australian National University, Acton, ACT, AustraliaJohn Curtin School of Medical Research, Australian National University, Acton, ACT, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Hinton
authors.affiliation: Immunology and Stem Cell Laboratory, Research School of Biology, Australian National University, Acton, ACT, AustraliaJohn Curtin School of Medical Research, Australian National University, Acton, ACT, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Papathanasiou
authors.affiliation: Immunology and Stem Cell Laboratory, Research School of Biology, Australian National University, Acton, ACT, AustraliaJohn Curtin School of Medical Research, Australian National University, Acton, ACT, Australia.
authors.forename: H C
authors.initials: HC
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000276:immunology; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D016130:Immunophenotyping; D008099:Liver / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D022423:Myeloid Cells / Q000166:cytology* / Q000276:immunology; D038042:Myelopoiesis / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D017154:Stromal Cells / Q000166:cytology / Q000276:immunology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers