title: Intraperitoneal oxygen/ozone treatment decreases the formation of experimental postsurgical peritoneal adhesions and the levels/activity of the local ubiquitin-proteasome system.
abstract: We have investigated whether an oxygen/ozone (95%O2/5%O3) mixture would have potential against the formation of experimental postsurgical peritoneal adhesions. In two groups of rats, one control intraperitoneally injected with 3 mL/rat of O2 and one intraperitoneally injected with oxygen/ozone mixture (3 mL/rat equivalent to 300 μg/kg ozone), we induced a midline laparotomy and an enterotomy at the level of the ileum to encourage the formation of peritoneal adhesions. Samples were taken from the parietal peritoneal tissue to assess the formation of adhesions 0 and 10 days after the surgical procedure and to assess the levels of ubiquitin and 20S proteasome. We found decreased formation of postsurgical peritoneal adhesions after treatment of the rats with 300 μg/kg ozone associated with a decreased levels of ubiquitin and 20S proteasome subunit within the adhered tissue. Oxygen/ozone mixture is potentially useful for approaching the post-surgical peritoneal adhesions, and the UPS system is involved in this.
authors.affiliation: Department of Experimental Medicine, Section of Pharmacology L. Donatelli, 2nd University of Naples, 80138 Naples, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Di Filippo
authors.forename: Annalisa
authors.initials: A
authors.lastname: Capuano
authors.forename: Barbara
authors.initials: B
authors.lastname: Rinaldi
authors.forename: Margherita
authors.initials: M
authors.lastname: Luongo
authors.forename: Biagio
authors.initials: B
authors.lastname: Lettieri
authors.forename: Francesco
authors.initials: F
authors.lastname: Rossi
authors.forename: Michele
authors.initials: M
authors.lastname: D'Amico
mesh_terms: D000818:Animals; D007813:Laparotomy / Q000009:adverse effects; D008297:Male; D010100:Oxygen / Q000627:therapeutic use*; D010126:Ozone / Q000627:therapeutic use*; D010532:Peritoneal Diseases / Q000188:drug therapy*; D011183:Postoperative Complications / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000188:drug therapy*
chemical_list: D010126:Ozone; D010100:Oxygen