title: Cutting edge: expression of XCR1 defines mouse lymphoid-tissue resident and migratory dendritic cells of the CD8α+ type.
abstract: Subsets of dendritic cells (DCs) have been described according to their functions and anatomical locations. Conventional DC subsets are defined by reciprocal expression of CD11b and CD8α in lymphoid tissues (LT), and of CD11b and CD103 in non-LT (NLT). Spleen CD8α(+) and dermal CD103(+) DCs share a high efficiency for Ag cross-presentation and a developmental dependency on specific transcription factors. However, it is not known whether all NLT-derived CD103(+) DCs and LT-resident CD8α(+) DCs are similar despite their different anatomical locations. XCR1 was previously described as exclusively expressed on mouse spleen CD8α(+) DCs and human blood BDCA3(+) DCs. In this article, we showed that LT-resident CD8α(+) DCs and NLT-derived CD103(+) DCs specifically express XCR1 and are characterized by a unique transcriptional fingerprint, irrespective of their tissue of origin. Therefore, CD8α(+) DCs and CD103(+) DCs belong to a common DC subset which is unequivocally identified by XCR1 expression throughout the body.
authors.affiliation: Centre d'Immunologie Marseille-Luminy, Université de la Méditerranée, 13288 Marseille, France. crozat@ciml.univ-mrs.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Crozat
authors.forename: Samira
authors.initials: S
authors.lastname: Tamoutounour
authors.forename: Thien-Phong
authors.initials: TP
authors.lastname: Vu Manh
authors.forename: Even
authors.initials: E
authors.lastname: Fossum
authors.forename: Hervé
authors.initials: H
authors.lastname: Luche
authors.forename: Laurence
authors.initials: L
authors.lastname: Ardouin
authors.forename: Martin
authors.initials: M
authors.lastname: Guilliams
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Azukizawa
authors.forename: Bjarne
authors.initials: B
authors.lastname: Bogen
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.forename: Sandrine
authors.initials: S
authors.lastname: Henri
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis; D016827:CD8 Antigens / Q000096:biosynthesis*; D002465:Cell Movement / Q000235:genetics / Q000276:immunology*; D016172:DNA Fingerprinting; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology / Q000276:immunology*; D005819:Genetic Markers / Q000276:immunology; D006801:Humans; D039001:Integrin alpha Chains / Q000096:biosynthesis; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000235:genetics; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000276:immunology
chemical_list: D015703:Antigens, CD; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D005819:Genetic Markers; D039001:Integrin alpha Chains; D019707:Receptors, Chemokine; D043562:Receptors, G-Protein-Coupled; C551408:XC chemokine receptor 1, mouse; C091671:XCR1 protein, human; C091367:alpha E integrins