title: [Screening PIAS2-interacting proteins in the mouse using the yeast two-hybrid system].
abstract: OBJECTIVE
  To screen and identify PIAS2-interacting proteins from the mouse spermatogonial cDNA library using the yeast two-hybrid system, and to investigate the action mechanism of PIAS2 in spermatogenesis.
  
  
  METHODS
  With pGBKT7-PIAS2 as a bait plasmid, the positive clones interacting with pGBKT7-PIAS2 were screened from the mouse spermatogonial cDNA library, the inserted fragments were sequenced and underwent bioinformatic analysis, and their interaction was verified using the yeast two-hybrid system.
  
  
  RESULTS
  Through screening, sequencing, homology analysis and yeast two-hybrid verification, we obtained 8 different candidate proteins interacting with PIAS2, including Cyfip2, Psmb3, Nmel, nischarin, Ints10, Nsun5, Gnb211 and Ndufaf3.
  
  
  CONCLUSIONS
  Eight different genes were successfully obtained using the yeast two-hybrid system, and their encoding proteins interacted with PIAS2, which might be related with male fertility regulation. Our findings have offered some new clues to the action mechanism of PIAS2 in spermatogenesis.
authors.affiliation: Department of Histology and Embryology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, China. yzzkl@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Jia
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Yan-Qiu
authors.initials: YQ
authors.lastname: Hu
mesh_terms: D000818:Animals; D001483:Base Sequence; D015723:Gene Library; D008297:Male; D051379:Mice; D011485:Protein Binding; D050828:Protein Inhibitors of Activated STAT / Q000235:genetics*; D054730:Protein Interaction Domains and Motifs; D013091:Spermatogenesis / Q000235:genetics*; D013093:Spermatogonia / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D050828:Protein Inhibitors of Activated STAT