title: MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients.
abstract: Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n=182) recovered from CF patients over a 15 year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database.
authors.affiliation: Servicio de Microbiología and CIBER en Epidemiología y Salud Pública (CIBERESP), Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), 28034 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Fernández-Olmos
authors.forename: María
authors.initials: M
authors.lastname: García-Castillo
authors.forename: María-Isabel
authors.initials: MI
authors.lastname: Morosini
authors.forename: Adelaida
authors.initials: A
authors.lastname: Lamas
authors.forename: Luis
authors.initials: L
authors.lastname: Máiz
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
mesh_terms: D042402:Achromobacter / Q000302:isolation & purification; D039903:Actinobacteria / Q000302:isolation & purification; D016956:Burkholderia cepacia / Q000302:isolation & purification; D045247:Chryseobacterium / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology*; D006088:Gram-Negative Aerobic Bacteria / Q000302:isolation & purification*; D006801:Humans; D040901:Proteomics; D011549:Pseudomonas / Q000302:isolation & purification; D049920:Ralstonia pickettii / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization