title: Interaction of porcine conventional dendritic cells with swine influenza virus.
abstract: Swine influenza virus (SwIV) causes sub-acute or acute respiratory infections on swine farms and pigs can act as "mixing vessels" for new influenza strains. Knowledge of the immune response of SwIV in its natural host, pigs, is very limited. Dendritic cells (DCs) mediate the induction of immunity to pathogens, but their interaction with SwIV has not been fully characterized. Thus, porcine bone marrow derived DCs (poBMDCs) were exposed to a circulating strain of H3N2 SwIV in vitro. Infection of poBMDCs resulted in structures resembling influenza virus inside poBMDCs in vesicles and also free in cytoplasm. Viral progeny was undetectable in supernatant but limited replication was detected in the first 8h after infection. However, viral particles from infected-poBMDCs were able to induce cytopathic effect in susceptible cells only when cell-to-cell interaction was favoured. The data generated in our studies reveal the particular interaction of H3N2 SwIV with conventional DCs.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Tufária
authors.initials: T
authors.lastname: Mussá
authors.forename: Carolina
authors.initials: C
authors.lastname: Rodriguez-Cariño
authors.forename: Myriam
authors.initials: M
authors.lastname: Pujol
authors.forename: Lorena
authors.initials: L
authors.lastname: Córdoba
authors.forename: Núria
authors.initials: N
authors.lastname: Busquets
authors.forename: Elisa
authors.initials: E
authors.lastname: Crisci
authors.forename: Javier
authors.initials: J
authors.lastname: Dominguez
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fraile
authors.forename: María
authors.initials: M
authors.lastname: Montoya
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001854:Bone Marrow Cells / Q000821:virology; D002450:Cell Communication; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000821:virology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000472:pathogenicity*; D013552:Swine
chemical_list: D000914:Antibodies, Viral