title: Hypermethylated DNA as potential biomarkers for gastric cancer diagnosis.
abstract: OBJECTIVE
  To demonstrate the diagnostic significance of methylation, an important molecular event in gastric carcinogenesis.
  
  
  METHODS
  We used methylation microarray to determine candidate genes, and performed MSP to evaluate the methylation status of them in tissues and sera. The effect of demethylation on mRNA expression was investigated by rt-PCR after gastric cancer cell lines were treated with 5-Aza-dC for 96 h.
  
  
  RESULTS
  In tissues and sera of gastric cancer patients, a higher prevalence of methylation was observed for BX141696, WT1, CYP26B1, and KCNA4, compared to healthy people (p<0.05, respectively). Detection of the methylation prevalence of KCNA4 and CYP26B1 together in serum demonstrated the good sensitivity (91.3%) and specificity (92.1%). After 5-Aza-dC treatment in gastric cancer cell lines, the mRNA expression level of these genes was restored.
  
  
  CONCLUSIONS
  This study underscores the potential application of measurement of serum DNA methylation of these genes, as promising tool for gastric cancer detection.
authors.affiliation: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Zheng
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Jianfang
authors.initials: J
authors.lastname: Li
authors.forename: Beiqin
authors.initials: B
authors.lastname: Yu
authors.forename: Liping
authors.initials: L
authors.lastname: Su
authors.forename: Xuehua
authors.initials: X
authors.lastname: Chen
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D002277:Carcinoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D003577:Cytochrome P-450 Enzyme System / Q000097:blood / Q000235:genetics; D004247:DNA / Q000097:blood* / Q000235:genetics; D019175:DNA Methylation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D006801:Humans; D051665:Kv1.4 Potassium Channel / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D000072516:Retinoic Acid 4-Hydroxylase; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D046888:Tissue Array Analysis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C496977:KCNA4 protein, human; D051665:Kv1.4 Potassium Channel; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; C000607079:CYP26B1 protein, human; D000072516:Retinoic Acid 4-Hydroxylase