title: Optimization of CO2 laser-based pretreatment of corn stover using response surface methodology.
abstract: CO2 laser pretreatment to improve the enzymatic hydrolysis of corn stover for production of monosaccharide, was investigated. Response surface methodology (RSM), at a three-variable, three-level experiment Box-Behnken design (BBD) established the following optimum pretreatment parameters: time, 67.53 min; power, 264.33 W; and liquid-to-solid ratio, 21.29:1 (mL/g). Under these conditions, the reducing sugars produced was 4.941 mg/mL for cellulase hydrolysis. This amount matched the predicted value and increased cellulase hydrolysis from 14.47% to 30.84%. Scanning electron microscopy (SEM) showed that CO2 laser pretreatment converted the smooth into a rough and porous surface, which promoted the enzyme access and resulted in a high specific reducing sugars production rate.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Shuang-Qi
authors.initials: SQ
authors.lastname: Tian
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Zi-Luan
authors.initials: ZL
authors.lastname: Fan
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zuo
mesh_terms: D000704:Analysis of Variance; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D006868:Hydrolysis; D054020:Lasers, Gas; D010084:Oxidation-Reduction; D013499:Surface Properties; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D014866:Waste Products