title: Protein trafficking rates assessed by quantum dot quenching with bromocresol green.
abstract: Quantum dots are bright, photostable fluorophores used extensively to investigate biological processes. In this study, we report that bromocresol green (BCG) at low micromolar concentrations rapidly, efficiently and reversibly quenches the fluorescence of commercial quantum dots having a wide range of functionalities. The broad utility of BCG quenching of quantum dots in cell biology is showed in quantitative assays of trafficking of the β(2) -adrenergic receptor (β(2) AR) and the cystic fibrosis transmembrane conductance regulator (CFTR).
authors.affiliation: Department of Medicine, University of California-San Francisco, CA 94143, USA.
authors.forename: Cathleen D
authors.initials: CD
authors.lastname: Valentine
authors.forename: A S
authors.initials: AS
authors.lastname: Verkman
authors.forename: Peter M
authors.initials: PM
authors.lastname: Haggie
mesh_terms: D000818:Animals; D001961:Bromcresol Green / Q000737:chemistry*; D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis; D005089:Exocytosis; D000882:Haplorhini; D006801:Humans; D007202:Indicators and Reagents; D008856:Microscopy, Fluorescence / Q000379:methods; D015394:Molecular Structure; D010316:Particle Size; D021381:Protein Transport; D045663:Quantum Dots; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D020349:Surface Plasmon Resonance / Q000379:methods; D014162:Transfection
chemical_list: D007202:Indicators and Reagents; D018343:Receptors, Adrenergic, beta-2; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D001961:Bromcresol Green