title: Development and evaluation of a selective medium for Brucella suis.
abstract: A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.
authors.affiliation: Instituto Nacional de Recursos Biológicos (INRB), I.P./LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal. Cristina.ferreira@lniv.min-agricultura.pt
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Ferreira
authors.forename: Cláudia
authors.initials: C
authors.lastname: Almendra
authors.forename: Regina
authors.initials: R
authors.lastname: Cardoso
authors.forename: Marta Silva
authors.initials: MS
authors.lastname: Pereira
authors.forename: Albano
authors.initials: A
authors.lastname: Beja-Pereira
authors.forename: Gordon
authors.initials: G
authors.lastname: Luikart
authors.forename: Maria Inácia
authors.initials: MI
authors.lastname: Corrêa de Sá
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000592:standards / Q000662:veterinary*; D041021:Brucella suis / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D002006:Brucellosis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D003470:Culture Media / Q000737:chemistry* / Q000592:standards; D024881:Drug Resistance, Bacterial; D008826:Microbial Sensitivity Tests; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media