title: High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection.
abstract: A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.
authors.affiliation: Genos Ltd, Laboratory for Glycobiology, Zagreb, Croatia.
authors.forename: Ana
authors.initials: A
authors.lastname: Knežević
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bones
authors.forename: Stjepan Krešimir
authors.initials: SK
authors.lastname: Kračun
authors.forename: Olga
authors.initials: O
authors.lastname: Gornik
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D006801:Humans; D011134:Polysaccharides / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D011134:Polysaccharides