title: The behavior of kinetic parameters in production of pectinase and xylanase by solid-state fermentation.
abstract: Solid-state fermentation (SSF) is defined as the growth of microbes without a free-flowing aqueous phase. The feasibility of using a citrus peel for producing pectinase and xylanase via the SSF process by Aspergillus niger F3 was evaluated in a 2 kg bioreactor. Different aeration conditions were tested to optimize the pectinase and xylanase production. The best air flow intensity was 1 V kg M (volumetric air flow per kilogram of medium), which allowed a sufficient amount of O2 for the microorganism growth producing 265 U/g and 65 U/g pectinases and xylanases, respectively. A mathematical model was applied to determine the different kinetic parameters related to SSF. The specific growth rate and biomass oxygen yield decreased during fermentation, whereas an increase in the maintenance coefficient for the different employed carbon sources was concurrently observed.
authors.affiliation: Biotechnology and Bioengineering Division, Federal University of Paraná (UFPR), CEP 81.531-970 Curitiba, PR, Brazil. danernesto72@yahoo.com
authors.forename: D E
authors.initials: DE
authors.lastname: Rodríguez-Fernández
authors.forename: J A
authors.initials: JA
authors.lastname: Rodríguez-León
authors.forename: J C
authors.initials: JC
authors.lastname: de Carvalho
authors.forename: W
authors.initials: W
authors.lastname: Sturm
authors.forename: C R
authors.initials: CR
authors.lastname: Soccol
mesh_terms: D000332:Aerobiosis; D000388:Air; D001234:Aspergillus niger / Q000201:enzymology*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000378:metabolism; D002957:Citrus / Q000737:chemistry; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D005285:Fermentation; D007700:Kinetics; D010101:Oxygen Consumption; D011096:Polygalacturonase / Q000096:biosynthesis*
chemical_list: D002245:Carbon Dioxide; D011096:Polygalacturonase; D043364:Endo-1,4-beta Xylanases