title: Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro.
abstract: Previous data demonstrate that JMJD2A is a cancer-associated gene and may be involved in human breast cancer by demethylation of H3K9me3. The aim of this study was to investigate depressive effects on JMJD2A by transfection with JMJD2A-sepcific siRNA in human breast cancer cell line MDA-MB-231 and effects on cell proliferation, invasion and migration. JMJD2A-specific siRNA was chemically synthesised and transfected into human breast cancer cell line MDA-MB-231. Expression levels of JMJD2A were detected by quantitative real-time PCR and Western blot analysis. Cells proliferation was evaluated by using flow cytometric anlysis and MTT assay. The abilities of invasion and migration were evaluated by cell migration and invasion assay with Boyden chambers. The results showed that the transfection was successful and expression levels of JMJD2A mRNA and protein in siRNA group were both down-regulated. By MTT assay, the mean actual absorbance in siRNA group was significantly lower than that in blank control group (P < 0.05) and negative control group (P < 0.05). In addition, the percentage of cells in G0/G1 phase in siRNA group was significantly more than that in blank control group (P < 0.05) and negative control group (P < 0.05). Furthermore, by cell invasion and migration assay, the decreased number of migrated cells in siRNA group was observed (P < 0.05). These data imply that silencing JMJD2A gene could result in cell cycle change and proliferation inhibition, and lead to suppress tumor cell invasion and migration. It provides a new perspective in understanding the pleiotropic functions of JMJD2A and its contribution to human breast cancer.
authors.affiliation: Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, PR China.
authors.forename: Bei-Xu
authors.initials: BX
authors.lastname: Li
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Zhang
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Luo
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Xu
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xu
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Shen
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Xue
authors.forename: Zi-Qin
authors.initials: ZQ
authors.lastname: Zhao
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D056484:Jumonji Domain-Containing Histone Demethylases / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; D056484:Jumonji Domain-Containing Histone Demethylases; C494424:KDM4A protein, human