title: Proteomic analysis of sera from common variable immunodeficiency patients undergoing replacement intravenous immunoglobulin therapy.
abstract: Common variable immunodeficiency is the most common form of symptomatic primary antibody failure in adults and children. Replacement immunoglobulin is the standard treatment of these patients. By using a differential proteomic approach based on 2D-DIGE, we examined serum samples from normal donors and from matched, naive, and immunoglobulin-treated patients. The results highlighted regulated expression of serum proteins in naive patients. Among the identified proteins, clusterin/ApoJ serum levels were lower in naive patients, compared to normal subjects. This finding was validated in a wider collection of samples from newly enrolled patients. The establishment of a cellular system, based on a human hepatocyte cell line HuH7, allowed to ascertain a potential role in the regulation of CLU gene expression by immunoglobulins.
authors.affiliation: Dipartimento di Medicina Clinica, Scienze Cardiovascolari e Immunologiche e Centro Interdipartimentale di Ricerca in Immunologia di Base e Clinica, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy. spadaro@unina.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Spadaro
authors.forename: Concetta
authors.initials: C
authors.lastname: D'Orio
authors.forename: Arturo
authors.initials: A
authors.lastname: Genovese
authors.forename: Antonella
authors.initials: A
authors.lastname: Galeotafiore
authors.forename: Chiara
authors.initials: C
authors.lastname: D'Ambrosio
authors.forename: Stefano
authors.initials: S
authors.lastname: Di Giovanni
authors.forename: Monica
authors.initials: M
authors.lastname: Vitale
authors.forename: Mario
authors.initials: M
authors.lastname: Capasso
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lamberti
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.forename: Gianni
authors.initials: G
authors.lastname: Marone
authors.forename: Nicola
authors.initials: N
authors.lastname: Zambrano
mesh_terms: D000328:Adult; D000906:Antibodies / Q000378:metabolism; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D051152:Clusterin / Q000235:genetics / Q000378:metabolism; D017074:Common Variable Immunodeficiency / Q000097:blood* / Q000628:therapy*; D005260:Female; D015870:Gene Expression; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D016756:Immunoglobulins, Intravenous / Q000627:therapeutic use*; D008297:Male; D040901:Proteomics
chemical_list: D000906:Antibodies; D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous