title: Oxidation of Akt2 kinase promotes cell migration and regulates G1-S transition in the cell cycle.
abstract: Phosphorylation has long been recognized as the key mediator of protein signaling. New modes of signaling regulation are emerging with the development of specific chemical probes and application of high-throughput mass spectrometry technologies. Using biotin-tagged chemical probes for protein oxidation, mass spectrometry and functional assays, our group has recently reported isoform-specific oxidation of Akt2 in response to PDGF signaling. The studies included here investigate the functional consequence of oxidation on Akt2-mediated cell migration and cell cycle. Akt2-KO MEFs transduced with WT and Cys124Ser Akt2 were used as the model system for these studies. The implications of these findings on disease pathology are discussed.
authors.affiliation: Section on Molecular Medicine, Department of Internal Medicine, Wake Forest School of Medicine, Winston-Salem, NC, USA.
authors.forename: Revati
authors.initials: R
authors.lastname: Wani
authors.forename: N Sharmila
authors.initials: NS
authors.lastname: Bharathi
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Field
authors.forename: Allen W
authors.initials: AW
authors.lastname: Tsang
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Furdui
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement; D005347:Fibroblasts / Q000166:cytology* / Q000201:enzymology* / Q000378:metabolism; D016193:G1 Phase; D051379:Mice; D010084:Oxidation-Reduction; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016196:S Phase; D015398:Signal Transduction
chemical_list: D010982:Platelet-Derived Growth Factor; D020033:Protein Isoforms; D051057:Proto-Oncogene Proteins c-akt