title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans.
abstract: Changes in glycosylation have long been associated with disease. While there are many methods to detect changes in glycosylation, plant derived lectins are often used to determine changes on specific proteins or molecules of interest. One change in glycosylation that has been observed by us and by others is a disease or antigen associated increase in fucosylation on N-linked glycans. To measure this change, the fucose binding Aleuria aurantia lectin (AAL) is often utilized in plate and solution based assays. AAL is a mushroom derived lectin that contains five fucose binding sites that preferentially bind fucose linked (α-1,3, α-1,2, α-,4, and α-1,6) to N-acetyllactosamine related structures. Recently, several reports by us and by others have indicated that specific fucose linkages found on certain serum biomarker glycoprotein's are more associated with disease than others. Taking a site-directed mutagenesis approach, we have created a set of recombinant AAL proteins that display altered binding affinities to different analytes containing various fucose linkages.
authors.affiliation: Drexel University College of Medicine, Department of Microbiology and Immunology and Drexel Institute for Biotechnology and Virology, 3805 Old Easton Avenue, Doylestown, PA 18901, USA.
authors.forename: Patrick R
authors.initials: PR
authors.lastname: Romano
authors.forename: Andrew
authors.initials: A
authors.lastname: Mackay
authors.forename: Minh
authors.initials: M
authors.lastname: Vong
authors.forename: Johann
authors.initials: J
authors.lastname: DeSa
authors.forename: Anne
authors.initials: A
authors.lastname: Lamontagne
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Comunale
authors.forename: Julie
authors.initials: J
authors.lastname: Hafner
authors.forename: Timothy
authors.initials: T
authors.lastname: Block
authors.forename: Ryszard
authors.initials: R
authors.lastname: Lec
authors.forename: Anand
authors.initials: A
authors.lastname: Mehta
mesh_terms: D000606:Amino Sugars / Q000737:chemistry; D005643:Fucose / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D011134:Polysaccharides / Q000737:chemistry; D011485:Protein Binding; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D000606:Amino Sugars; D037102:Lectins; D011134:Polysaccharides; D011994:Recombinant Proteins; C064549:lectin, Aleuria aurantia; D005643:Fucose; C000458:N-acetyllactosamine