title: Immunohistochemical expression and prognostic significance of CCND3, MCM2 and MCM7 in Hodgkin lymhoma.
abstract: BACKGROUND
  Increased proliferation rate may be associated with inferior outcome in patients with Hodgkin lymphoma (HL). Minichromosome maintenance proteins (MCMs) and D-type cyclins are essential for DNA replication.
  
  
  METHODS
  Lymph node sections from 138 HL patients were immunohistochemically stained for cyclin D3 (CCND3), MCM2 and MCM7 aiming to investigate clinical outcome.
  
  
  RESULTS
  Higher MCM2 expression was observed in patients in early stage disease and normal albumin levels; higher MCM7 was found for asymptomatic patients, early stage disease, <5 involved sites, no anemia and normal albumin levels; higher CCND3 expression was found for older patients and normal lactate dehydrogenase (LDH). Univariate analysis revealed no correlation with failure-free (FFS) or overall survival (OS). Multivariate analysis revealed that high MCM7 expression was an adverse prognostic factor for OS, along with older age and advanced stage, while it was of borderline significance for FFS when adjusted for stage.
  
  
  CONCLUSIONS
  These results suggest that MCM7 deserves further evaluation as a potential independent prognostic factor in larger patient series.
authors.affiliation: Department of Pathology, Laikon General Hospital, National and Kapodistrian University of Athens, Greece.
authors.forename: Andreas
authors.initials: A
authors.lastname: Marnerides
authors.forename: Theodoros P
authors.initials: TP
authors.lastname: Vassilakopoulos
authors.forename: Evmorfia
authors.initials: E
authors.lastname: Boltetsou
authors.forename: Georgia
authors.initials: G
authors.lastname: Levidou
authors.forename: Maria K
authors.initials: MK
authors.lastname: Angelopoulou
authors.forename: Irene
authors.initials: I
authors.lastname: Thymara
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Kyrtsonis
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Pappi
authors.forename: Olga
authors.initials: O
authors.lastname: Tsopra
authors.forename: Panayiotis
authors.initials: P
authors.lastname: Panayiotidis
authors.forename: Gerassimos A
authors.initials: GA
authors.lastname: Pangalis
authors.forename: Photis
authors.initials: P
authors.lastname: Beris
authors.forename: Efstratios
authors.initials: E
authors.lastname: Patsouris
authors.forename: Penelope
authors.initials: P
authors.lastname: Korkolopoulou
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism*; D049109:Cell Proliferation; D056743:Cyclin D3 / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018572:Disease-Free Survival; D005260:Female; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7; D015999:Multivariate Analysis; D009687:Nuclear Proteins / Q000378:metabolism*; D011379:Prognosis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C534696:CCND3 protein, human; D018797:Cell Cycle Proteins; D056743:Cyclin D3; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C084859:MCM2 protein, human; C101624:MCM7 protein, human; D064111:Minichromosome Maintenance Complex Component 2; D064168:Minichromosome Maintenance Complex Component 7