title: Polyelectrolyte complex of carboxymethyl starch and chitosan as protein carrier: oral administration of ovalbumin.
abstract: A novel carboxymethyl starch (CMS)/chitosan polyelectrolyte complex (PEC) was proposed as an excipient for oral administration of ovalbumin. The dissolution of ovalbumin from monolithic tablets (200 mg, 2.1 × 9.6 mm, 50% loading) obtained by direct compression was studied. When CMS was used as an excipient, more than 70% of the loaded ovalbumin remained undigested after 1 h of incubation in simulated gastric fluid (SGF) with pepsin. The complete dissolution, after transfer of tablets into simulated intestinal fluid (SIF) with pancreatin, occurred within a total time of about 6 h. Higher protection (more than 90% stability in SGF) and longer dissolution (more than 13 h) were obtained with 50% CMS/50% chitosan physical mixture or with PEC excipients. A lower proportion of chitosan was needed for PEC than for the CMS/chitosan mixture to obtain a similar dissolution profile. The high protection against digestion by pepsin, the various release times and the mucoadhesion properties of these excipients based on CMS favor the development of suitable carriers for oral vaccinations.
authors.affiliation: a Department of Chemistry and Pharmaqam Center , Université du Québec à Montréal (UQAM) , CP 8888, Succ. Centre-ville , Montréal , QC , Canada , H3C 3P8.
authors.forename: Elias
authors.initials: E
authors.lastname: Assaad
authors.forename: Lindsay
authors.initials: L
authors.lastname: Blemur
authors.forename: Martin
authors.initials: M
authors.lastname: Lessard
authors.forename: Mircea Alexandru
authors.initials: MA
authors.lastname: Mateescu
mesh_terms: D000284:Administration, Oral; D015153:Blotting, Western; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry; D003101:Collodion / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005079:Excipients / Q000737:chemistry; D005750:Gastric Juice / Q000737:chemistry; D008567:Membranes, Artificial; D009077:Mucins / Q000737:chemistry; D010047:Ovalbumin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D010194:Pancreatin / Q000737:chemistry; D010434:Pepsin A / Q000737:chemistry; D013213:Starch / Q000031:analogs & derivatives* / Q000737:chemistry; D013607:Tablets / Q000737:chemistry; D013997:Time Factors
chemical_list: D005079:Excipients; D008567:Membranes, Artificial; D009077:Mucins; D013607:Tablets; D010194:Pancreatin; D003101:Collodion; D013213:Starch; D010047:Ovalbumin; D048271:Chitosan; C034848:carboxymethyl starch; D010434:Pepsin A