title: Parametric optimization of feruloyl esterase production from Aspergillus terreus strain GA2 isolated from tropical agro-ecosystems cultivating sweet sorghum.
abstract: A fungal strain, Aspergillus terreus strain GA2, isolated from an agricultural field cultivating sweet sorghum, produced feruloyl esterase using maize bran. In order to obtain maximum yields of feruloyl esterase, the solid state fermentation (SSF) conditions for enzyme production were standardized. Effective feruloyl esterase production was observed with maize bran as substrate followed by wheat bran, coconut husk, and rice husk among the tested agro-waste crop residues. Optimum particle size of 0.71- 0.3 mm and moisture content of 80% favored enzyme production. Moreover, optimum feruloyl esterase production was observed at pH 6.0 and a temperature of 30 degrees C. Supplementation of potato starch (0.6%) as the carbon source and casein (1%) as the nitrogen source favored enzyme production. Furthermore, the culture produced the enzyme after 7 days of incubation when the C:N ratio was 5. Optimization of the SSF conditions revealed that maximum enzyme activity (1,162 U/gds) was observed after 7 days in a production medium of 80% moisture content and pH 6.0 containing 16 g maize bran [25% (w/v)] of particle size of 0.71-0.3 mm, 0.6% potato starch, 3.0% casein, and 64 ml of formulated basal salt solution. Overall, the enzyme production was enhanced by 3.2-fold as compared with un-optimized conditions.
authors.affiliation: Chemical Biology Laboratory, Indian Institute of Chemical Technology, Hyderabad 500 607, India. cgkumar@iict.res.in
authors.forename: C Ganesh
authors.initials: CG
authors.lastname: Kumar
authors.forename: Avijeet
authors.initials: A
authors.lastname: Kamle
authors.forename: Poornima
authors.initials: P
authors.lastname: Mongolla
authors.forename: Joveeta
authors.initials: J
authors.lastname: Joseph
mesh_terms: D001230:Aspergillus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D017753:Ecosystem; D005285:Fermentation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007194:India; D045868:Sorghum / Q000254:growth & development / Q000382:microbiology*; D014329:Tropical Climate
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase