title: Engineering topology and kinetics of sucrose metabolism in Saccharomyces cerevisiae for improved ethanol yield.
abstract: Sucrose is a major carbon source for industrial bioethanol production by Saccharomyces cerevisiae. In yeasts, two modes of sucrose metabolism occur: (i) extracellular hydrolysis by invertase, followed by uptake and metabolism of glucose and fructose, and (ii) uptake via sucrose-proton symport followed by intracellular hydrolysis and metabolism. Although alternative start codons in the SUC2 gene enable synthesis of extracellular and intracellular invertase isoforms, sucrose hydrolysis in S. cerevisiae predominantly occurs extracellularly. In anaerobic cultures, intracellular hydrolysis theoretically enables a 9% higher ethanol yield than extracellular hydrolysis, due to energy costs of sucrose-proton symport. This prediction was tested by engineering the promoter and 5' coding sequences of SUC2, resulting in predominant (94%) cytosolic localization of invertase. In anaerobic sucrose-limited chemostats, this iSUC2-strain showed an only 4% increased ethanol yield and high residual sucrose concentrations indicated suboptimal sucrose-transport kinetics. To improve sucrose-uptake affinity, it was subjected to 90 generations of laboratory evolution in anaerobic, sucrose-limited chemostat cultivation, resulting in a 20-fold decrease of residual sucrose concentrations and a 10-fold increase of the sucrose-transport capacity. A single-cell isolate showed an 11% higher ethanol yield on sucrose in chemostat cultures than an isogenic SUC2 reference strain, while transcriptome analysis revealed elevated expression of AGT1, encoding a disaccharide-proton symporter, and other maltose-related genes. After deletion of both copies of the duplicated AGT1, growth characteristics reverted to that of the unevolved SUC2 and iSUC2 strains. This study demonstrates that engineering the topology of sucrose metabolism is an attractive strategy to improve ethanol yields in industrial processes.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Kluyver Centre for Genomics of Industrial Fermentation, Delft, The Netherlands.
authors.forename: Thiago O
authors.initials: TO
authors.lastname: Basso
authors.forename: Stefan
authors.initials: S
authors.lastname: de Kok
authors.forename: Marcelo
authors.initials: M
authors.lastname: Dario
authors.forename: Júlio Cézar A
authors.initials: JC
authors.lastname: do Espirito-Santo
authors.forename: Gabriela
authors.initials: G
authors.lastname: Müller
authors.forename: Paulo S
authors.initials: PS
authors.lastname: Schlölg
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Silva
authors.forename: Aldo
authors.initials: A
authors.lastname: Tonso
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Daran
authors.forename: Andreas K
authors.initials: AK
authors.lastname: Gombert
authors.forename: Antonius J A
authors.initials: AJ
authors.lastname: van Maris
authors.forename: Jack T
authors.initials: JT
authors.lastname: Pronk
authors.forename: Boris U
authors.initials: BU
authors.lastname: Stambuk
mesh_terms: D005075:Biological Evolution; D000431:Ethanol / Q000378:metabolism*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis; D011401:Promoter Regions, Genetic; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics*; D013395:Sucrose / Q000378:metabolism*; D027981:Symporters / Q000096:biosynthesis; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D000431:Ethanol; D013395:Sucrose; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase