title: Association of B3GNT5 polymorphisms with susceptibility to ETEC F4ab/ac in the white Duroc × Erhualian intercross and 15 outbred pig breeds.
abstract: The B3GNT5 gene is a candidate for the F4ab/ac receptor conferring susceptibility to enterotoxigenic Escherichia coli (ETEC) F4ab/ac in pigs. In this study, we screened mutations in the complete coding region of the porcine B3GNT5 gene and identified four SNPs in the 3' untranslated regions. We genotyped the four SNPs across a large-scale White Duroc × Chinese Erhualian F2 resource population (total F2 = 755) and 292 purebred piglets representing 15 Chinese and Western breeds. We found that the g.1476G→A locus and haplotypes [A;T;G;T] and [A;G;G;T] had significant association with susceptibility to ETEC F4ac in the resource population. None of the B3GNT5 polymorphisms and haplotypes was associated with susceptibility to ETEC F4ab/ac in outbred piglets. This result, together with other reports, supports the conclusion that B3GNT5 is not the responsible gene encoding the ETEC F4ab/ac receptors.
authors.affiliation: Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ouyang
authors.forename: Weihong
authors.initials: W
authors.lastname: Zeng
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.forename: Xueming
authors.initials: X
authors.lastname: Yan
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Han
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.forename: Huashui
authors.initials: H
authors.lastname: Ai
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D021461:Animals, Outbred Strains; D054307:Enterotoxigenic Escherichia coli / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000235:genetics / Q000662:veterinary*; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D034421:Sus scrofa / Q000235:genetics*; D013552:Swine / Q000235:genetics; D013553:Swine Diseases / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D017351:N-Acetylglucosaminyltransferases