title: Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes.
abstract: Cultivated chickpea is the third most important legume after field bean and garden pea worldwide. Despite considerable breeding towards improved yield and resistance to biotic and abiotic stresses, the production of chickpea remained stagnant, but molecular tools are expected to increase the impact of current improvement programs. As a first step towards this goal, various genetic linkage maps have been established and markers linked to resistance genes been identified. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. This approach confirmed the assignment of LG8 to the smallest chromosome H. For the first time, LG5 was linked to the largest chromosome A, LG4 to a medium-sized chromosome E, while LG3 was anchored to the second largest chromosome B. Chromosomes C and D could not be flow-sorted separately and were jointly associated to LG6 and LG7. By the same token, chromosomes F and G were anchored to LG1 and LG2. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones were mapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.
authors.affiliation: Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Sokolovská 6, 77200 Olomouc, Czech Republic.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Zatloukalová
authors.forename: Eva
authors.initials: E
authors.lastname: Hřibová
authors.forename: Marie
authors.initials: M
authors.lastname: Kubaláková
authors.forename: Pavla
authors.initials: P
authors.lastname: Suchánková
authors.forename: Hana
authors.initials: H
authors.lastname: Simková
authors.forename: Cabrera
authors.initials: C
authors.lastname: Adoración
authors.forename: Günter
authors.initials: G
authors.lastname: Kahl
authors.forename: Teresa
authors.initials: T
authors.lastname: Millán
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D022202:Chromosomes, Artificial, Bacterial; D032461:Chromosomes, Plant / Q000235:genetics*; D029863:Cicer / Q000235:genetics*; D003582:Cytogenetics / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D005434:Flow Cytometry; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers