title: Analysis of HDAC1-mediated regulation of Runx2-induced osteopontin gene expression in C3h10t1/2 cells.
abstract: Histone deacetylases (HDACs) deacetylate lysine residues of histone and non-histone proteins and thereby regulate the cell-cycle, gene expression, and several other processes. We have analyzed the effects of HDAC1 on Runx2-mediated regulation of osteopontin (OPN) promoter activation and gene expression in mesenchymal progenitor C3h10t1/2 cells and show that co-expression of HDAC1 along with Runx2 results in down-regulation of Runx2-induced OPN mRNA expression during both the proliferation and differentiation stages of C3h10t1/2 cells. Luciferase assay results revealed that HDAC1 efficiently down-regulated Runx2-stimulated OPN promoter activity in a dose-dependent manner whereas TSA relieved the HDAC1-mediated repression and up-regulated the Runx2-induced OPN promoter activity and mRNA expression. In vivo HDAC1 co-localized and physically interacted with Runx2 and associated with the OPN promoter. Thus, HDAC1 not only plays a critical role in regulation of Runx2-stimulated expression of osteogenic genes, like OPN, but also regulate the proliferation and differentiation stages of mesenchymal progenitor cells, such as C3h10t1/2.
authors.affiliation: Institute of Genetics and Cell Biology, Northeast Normal University, Changchun, 130024, People's Republic of China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Zhang
authors.forename: Vishwa
authors.initials: V
authors.lastname: Deepak
authors.forename: Lingyi
authors.initials: L
authors.lastname: Meng
authors.forename: Linghui
authors.initials: L
authors.lastname: Wang
authors.forename: Yushan
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.forename: Xianlu
authors.initials: X
authors.lastname: Zeng
authors.forename: Wenguang
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D019677:Artificial Gene Fusion; D002460:Cell Line; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D056284:Histone Deacetylase 1 / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D053495:Osteopontin / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D025941:Protein Interaction Mapping; D014158:Transcription, Genetic
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493716:Runx2 protein, mouse; C506896:Spp1 protein, mouse; D053495:Osteopontin; D008156:Luciferases; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1