title: Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture.
abstract: Three Gram-negative bacterial strains were isolated from the biofilter of a recirculating marine aquaculture. They were non-pigmented rods, mesophiles, moderately halophilic, and showed chemo-organoheterotrophic growth on various sugars, fatty acids, and amino acids, with oxygen as electron acceptor; strains D9-3(T) and D11-58 were in addition able to denitrify. Phototrophic or fermentative growth could not be demonstrated. Phylogenetic analysis of the 16S rRNA gene sequences placed D9-3(T) and D11-58, and D1-19(T) on two distinct branches within the alpha-3 proteobacterial Rhodobacteraceae, affiliated with, but clearly separate from, the genera Rhodobacter, Rhodovulum, and Rhodobaca. Based on morphological, physiological, and 16S rRNA-based phylogenetic characteristics, the isolated strains are proposed as new species of two novel genera, Defluviimonas denitrificans gen. nov., sp. nov. (type strain D9-3(T)=DSM 18921(T)=ATCC BAA-1447(T); additional strain D11-58=DSM19039=ATCC BAA-1448) and Pararhodobacter aggregans gen. nov., sp. nov (type strain D1-19(T)=DSM 18938(T)=ATCC BAA-1446(T)).
authors.affiliation: Department of Ecological Microbiology, University of Bayreuth, Bayreuth, Germany.
authors.forename: Bärbel U
authors.initials: BU
authors.lastname: Foesel
authors.forename: Harold L
authors.initials: HL
authors.lastname: Drake
authors.forename: Andreas
authors.initials: A
authors.lastname: Schramm
mesh_terms: D017756:Aquaculture; D001482:Base Composition; D050260:Carbohydrate Metabolism; D058440:Denitrification; D005227:Fatty Acids / Q000502:physiology; D005798:Genes, Bacterial; D020459:Genes, rRNA; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D021521:Ribotyping; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S