title: Detection of growth-related QTL in turbot (Scophthalmus maximus).
abstract: BACKGROUND
  The turbot (Scophthalmus maximus) is a highly appreciated European aquaculture species. Growth related traits constitute the main goal of the ongoing genetic breeding programs of this species. The recent construction of a consensus linkage map in this species has allowed the selection of a panel of 100 homogeneously distributed markers covering the 26 linkage groups (LG) suitable for QTL search. In this study we addressed the detection of QTL with effect on body weight, length and Fulton's condition factor.
  
  
  RESULTS
  Eight families from two genetic breeding programs comprising 814 individuals were used to search for growth related QTL using the panel of microsatellites available for QTL screening. Two different approaches, maximum likelihood and regression interval mapping, were used in order to search for QTL. Up to eleven significant QTL were detected with both methods in at least one family: four for weight on LGs 5, 14, 15 and 16; five for length on LGs 5, 6, 12, 14 and 15; and two for Fulton's condition factor on LGs 3 and 16. In these LGs an association analysis was performed to ascertain the microsatellite marker with the highest apparent effect on the trait, in order to test the possibility of using them for marker assisted selection.
  
  
  CONCLUSIONS
  The use of regression interval mapping and maximum likelihood methods for QTL detection provided consistent results in many cases, although the high variation observed for traits mean among families made it difficult to evaluate QTL effects. Finer mapping of detected QTL, looking for tightly linked markers to the causative mutation, and comparative genomics are suggested to deepen in the analysis of QTL in turbot so they can be applied in marker assisted selection programs.
authors.affiliation: Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Sánchez-Molano
authors.forename: Alex
authors.initials: A
authors.lastname: Cerna
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Toro
authors.forename: Carmen
authors.initials: C
authors.lastname: Bouza
authors.forename: Miguel
authors.initials: M
authors.lastname: Hermida
authors.forename: Belén G
authors.initials: BG
authors.lastname: Pardo
authors.forename: Santiago
authors.initials: S
authors.lastname: Cabaleiro
authors.forename: Jesús
authors.initials: J
authors.lastname: Fernández
authors.forename: Paulino
authors.initials: P
authors.lastname: Martínez
mesh_terms: D000818:Animals; D001835:Body Weight; D001947:Breeding; D002874:Chromosome Mapping; D005412:Flatfishes / Q000235:genetics* / Q000254:growth & development; D008040:Genetic Linkage; D016013:Likelihood Functions; D018895:Microsatellite Repeats; D010641:Phenotype; D040641:Quantitative Trait Loci; D012044:Regression Analysis