title: Quantum dot-enhanced chemiluminescence detection for simultaneous determination of dopamine and epinephrine by capillary electrophoresis.
abstract: A sensitive method based on quantum dot (QD)-enhanced capillary electrophoresis-chemiluminescence (CE-CL) detection was developed for simultaneous determination of dopamine (DA) and epinephrine (E). In this work, CdTe QD was added into the running buffer of CE to catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving higher CL emission. Negative peaks were produced due to the inhibitory effects on CL emission from DA and E eluted from the electrophoretic capillary. The decrease in CL intensity was proportional to the concentration of DA and E in the range of 8.0 × 10(-8)-5.0 × 10(-6)M and 4.0 × 10(-8)-5.0 × 10(-6)M, respectively. Detection limits for DA and E were 2.3 × 10(-8)M and 9.3 × 10(-9)M, respectively. Using this method, the levels of DA and E in human urine from healthy donors were determined.
authors.affiliation: Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004, China.
authors.forename: Yunsha
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhao
authors.forename: Junming
authors.initials: J
authors.lastname: Huang
authors.forename: Fanggui
authors.initials: F
authors.lastname: Ye
mesh_terms: D004298:Dopamine / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004837:Epinephrine / Q000032:analysis*; D006801:Humans; D049449:Luminescence; D045663:Quantum Dots
chemical_list: D004298:Dopamine; D004837:Epinephrine