title: Proteomic profiling of the influence of iron availability on Cryptococcus gattii.
abstract: Iron is essential and ubiquitous in living organisms. The competition for this micronutrient between the host and its pathogens has been related to disease establishment. Cryptococcus gattii is an encapsulated yeast that causes cryptococcosis mainly in immunocompetent individuals. In this study, we analyzed the proteomic profile of the C. gattii R265 Vancouver Island isolate under iron-depleted and -repleted conditions by multidimensional protein identification technology (MudPIT) and by 2D-GE. Proteins and key mechanisms affected by alteration of iron levels such as capsule production, cAMP-signaling pathway, response to stress, and metabolic pathways related to mitochondrial function were identified. Our results also show both proteomic methodologies employed to be complementary.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 43421, Caixa Postal 15005, Porto Alegre, RS 91501-970, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Crestani
authors.forename: Paulo Costa
authors.initials: PC
authors.lastname: Carvalho
authors.forename: Xuemei
authors.initials: X
authors.lastname: Han
authors.forename: Adriana
authors.initials: A
authors.lastname: Seixas
authors.forename: Leonardo
authors.initials: L
authors.lastname: Broetto
authors.forename: Juliana de Saldanha da Gama
authors.initials: Jde S
authors.lastname: Fischer
authors.forename: Charley Christian
authors.initials: CC
authors.lastname: Staats
authors.forename: Augusto
authors.initials: A
authors.lastname: Schrank
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Marilene Henning
authors.initials: MH
authors.lastname: Vainstein
mesh_terms: D053898:Biosynthetic Pathways; D056285:Cryptococcus gattii / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015966:Gene Expression Regulation, Fungal; D007501:Iron / Q000378:metabolism / Q000502:physiology*; D058977:Molecular Sequence Annotation; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics
chemical_list: D005656:Fungal Proteins; D020543:Proteome; D007501:Iron