title: Molecular cloning, expression analysis and characterization of peroxiredoxin during WSSV infection in shrimp Fenneropenaeus indicus.
abstract: Innate immunity is the first line of defense in shrimps against invading pathogenic microorganisms. Peroxiredoxins (PRX) are the family of antioxidant proteins that play a crucial role in reduction of oxidative stress in host during viral infection. Peroxiredoxin from Fenneropenaeus indicus was identified, cloned and expressed in prokaryotic expression system. The Fi-PRX protein consists of two conserved Cys residues and belongs to typical 2-cys PRX family. The phylogenetic analysis revealed a close evolutionary relatedness of Fi-PRX with the PRX from Drosophila melanogaster PRX1 and distant origin with PRX sequences from other shrimp isolates Fenneropenaeus chinensis, Litopenaeus vannamei and Penaeus japonicus. Fi-PRX transcripts are constitutively expressed in hemocytes and tissues (gills, heart and muscle) and down regulated during 12 h, 24 h and 48 h of WSSV challenged shrimps. Fi-PRX protein levels correlated well with the corresponding levels of Fi-PRX transcripts in hemocytes and tissues of WSSV challenged shrimps. Recombinant Fi-PRX reduces insulin only in the presence of DTT suggesting that the antioxidant function of the protein is thiol dependent. These findings suggest that antioxidant activity of Fi-PRX play a significant role in neutralization of excessive free radicals and ROS generated during viral invasion.
authors.affiliation: Centre for Biotechnology, Anna University, Chennai 600 025, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Kiruthiga
authors.forename: S
authors.initials: S
authors.lastname: Rajesh
authors.forename: V
authors.initials: V
authors.lastname: Rashika
authors.forename: R
authors.initials: R
authors.lastname: Priya
authors.forename: R B
authors.initials: RB
authors.lastname: Narayanan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D004266:DNA Virus Infections / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D004229:Dithiothreitol / Q000378:metabolism; D019143:Evolution, Molecular; D015870:Gene Expression; D005880:Gills / Q000201:enzymology / Q000821:virology; D006321:Heart / Q000821:virology; D006434:Hemocytes / Q000201:enzymology / Q000821:virology; D007328:Insulin / Q000378:metabolism; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology / Q000821:virology; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D033561:Penaeidae / Q000201:enzymology / Q000276:immunology / Q000821:virology*; D054464:Peroxiredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000494:pharmacology; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000472:pathogenicity*
chemical_list: D000975:Antioxidants; D007328:Insulin; D011994:Recombinant Proteins; D054464:Peroxiredoxins; D004229:Dithiothreitol