title: Efficient bioconversion of compactin to pravastatin by the quinoline-degrading microorganism Pseudonocardia carboxydivorans isolated from petroleum-contaminated soil.
abstract: Pravastatin is one of the first available statins on the market. The purpose of this study was to isolate and identify the quinoline-degrading microorganism from petroleum-contaminated soil that could bioconvert compactin to pravastatin. There were 10,011 microorganism colonies isolated; five strains showed a higher capability for quinoline biodegradation. These five strains were evaluated for their pravastatin bioconversion ability; Pseudonocardia sp. had the highest efficiency for conversion of compactin to pravastatin. The strain was further identified as Pseudonocardia carboxydivorans PAH4. The bioconversion rates were studied under difference incubation conditions. Pre-incubation in medium containing 0.005% compactin sodium, resulted in the compactin utilization rate of almost 100% in a 1mg/ml compactin-containing medium. The rate of conversion of pravastatin was up to 68% after 6 days of incubation. In conclusion, the results of this study suggest that P. carboxydivorans PAH4 could be considered a candidate for the production of pravastatin on an industrial scale.
authors.affiliation: Refining & Manufacturing Research Institute, CPC Corporation, Taiwan, No. 217, Min-Sheng S. Rd, Chiayi 60051, Taiwan, ROC.
authors.forename: Chung-Liang
authors.initials: CL
authors.lastname: Lin
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Tang
authors.forename: Shyh-Mirn
authors.initials: SM
authors.lastname: Lin
mesh_terms: D000192:Actinomycetales / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D004275:DNA, Ribosomal / Q000235:genetics; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000378:metabolism; D008969:Molecular Sequence Data; D059392:Petroleum Pollution; D010802:Phylogeny; D017035:Pravastatin / Q000378:metabolism*; D011804:Quinolines / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D003470:Culture Media; D004275:DNA, Ribosomal; D011804:Quinolines; C012258:mevastatin; D008148:Lovastatin; D017035:Pravastatin