title: Oxidation of polyaromatic hydrocarbons in systems containing water miscible organic solvents by the lignin peroxidase of Gleophyllum striatum MTCC-1117.
abstract: Lignin peroxidase has been purified to homogeneity using a process of concentration by ultrafiltration and anion exchange chromatography on diethylaminoethyl (DEAE) cellulose from the liquid culture filtrate of the brown rot fungi Gleophyllum striatum MTCC-1117. The molecular mass of the purified enzyme is 43 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The K(m) values for the enzyme using veratryl alcohol, hydrogen peroxide and n-propanol were 66 microM, 82 microM and 476 microM, respectively. The pH and temperature optima of the enzyme were 2.8 and 25 degrees C, respectively. The enzyme is completely inhibited by 20% of the water miscible organic solvents acetone dioxane, diethylether, acetonitrile and dimethylformamide. The lignin peroxidase oxidizes polycyclic aromatic hydrocarbons pyrene, acenaphthene, anthracene, dibenothiophene and 9-methyl anthracene.
authors.affiliation: Department of Chemistry, DDU Gorakhpur University, Gorakhpur 273009, India. drmeerayadav@rediffmail.com
authors.forename: M
authors.initials: M
authors.lastname: Yadav
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.forename: J K
authors.initials: JK
authors.lastname: Sharma
authors.forename: K D S
authors.initials: KD
authors.lastname: Yadav
mesh_terms: D001487:Basidiomycota / Q000201:enzymology* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry* / Q000378:metabolism; D012997:Solvents; D013696:Temperature; D014462:Ultrafiltration
chemical_list: D005656:Fungal Proteins; D007220:Industrial Waste; D011084:Polycyclic Aromatic Hydrocarbons; D012997:Solvents; D010544:Peroxidases; C042858:lignin peroxidase