title: Response of Lactobacillus casei BL23 to phenolic compounds.
abstract: OBJECTIVE
  To determine the inhibitory effect of phenolic compounds on Lactobacillus casei BL23, the role of two component signal transduction systems (TCS) and the response of Lact. casei BL23 to p-coumaric acid.
  
  
  RESULTS
  Growth of Lact. casei BL23 and 17 derivative strains defective in each TCS harboured by this strain in the presence of p-coumaric acid, ferulic acid, caffeic acid or methyl gallate was monitored. Furthermore, changes in the protein content of Lact. casei BL23 when exposed to p-coumaric acid were evaluated by 2D-SDS-PAGE. Eleven proteins differentially expressed in the presence of p-coumaric acid were detected. Six of them could be identified: ClpP and HtrA, involved in protein turnover and folding, acetyl-CoA carboxylase, involved in lipid metabolism, and an arginyl-tRNA synthetase were more abundant, whereas PurL and PurN, involved in purine biosynthesis, were less abundant.
  
  
  CONCLUSIONS
  No significant differences were observed between the parental strain and the TCS-defective mutants. p-Coumaric acid elicited a response against membrane and cytoplasmic damages.
  
  
  CONCLUSIONS
  The inhibitory effect of phenolic compounds on Lact. casei BL23 has been determined. For the first time, cytoplasmic proteins presumably involved in the response of Lact. casei BL23 against p-coumaric acid have been identified.
authors.affiliation: Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), C/Catedrático Agustín Escardino, Paterna, Valencia, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rivas-Sendra
authors.forename: J M
authors.initials: JM
authors.lastname: Landete
authors.forename: C
authors.initials: C
authors.lastname: Alcántara
authors.forename: M
authors.initials: M
authors.lastname: Zúñiga
mesh_terms: D002109:Caffeic Acids / Q000494:pharmacology; D003373:Coumaric Acids / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D007780:Lacticaseibacillus casei / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology*; D011422:Propionates; D020543:Proteome / Q000032:analysis; D015398:Signal Transduction
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D003470:Culture Media; D010636:Phenols; D011422:Propionates; D020543:Proteome; C052082:methyl gallate; D005707:Gallic Acid; C004999:ferulic acid; C495469:p-coumaric acid; C040048:caffeic acid