title: Molecular structure and characterization of the cytokine TWEAK and its receptor Fn14 in bovine.
abstract: Full-length cDNA encoding TWEAK and Fn14 from bovine was isolated. We used bioinformatics to analyze the gene structure, function, evolutionary relationships, and predicted three-dimensional structure of proteins and binding sites. Real-time quantitative PCR (Q-PCR) analysis revealed that both TWEAK and Fn14 are constitutively expressed in various tissues in bovine. Bovine TWEAK and Fn14 interaction analysis by yeast two-hybrid. Our results suggest that the TWEAK-Fn14 pathway is evolutionarily highly conserved. It will be helpful for investigation on the biological role of the TWEAK/Fn14 system in this important animal model. Furthermore, it provides insight into the molecular evolution of the emerging TWEAK and Fn14 families.
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210097, PR China.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Sang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Ma
authors.forename: Cui
authors.initials: C
authors.lastname: Min
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Hu
authors.forename: Ming-Xiang
authors.initials: MX
authors.lastname: Du
authors.forename: Shuang-Quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D060888:Real-Time Polymerase Chain Reaction; D018124:Receptors, Tumor Necrosis Factor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000074050:TWEAK Receptor; D014018:Tissue Distribution; D048069:Tumor Necrosis Factors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D018124:Receptors, Tumor Necrosis Factor; D000074050:TWEAK Receptor; D048069:Tumor Necrosis Factors