title: Effects of alcohol consumption on biomarkers of oxidative damage to DNA and lipids in ethanol-fed pigs.
abstract: Chronic alcohol consumption is known to result in tissue injury, particularly in the liver, and is considered a major risk factor for cancers of the upper respiratory tract. Here we assessed the oxidative effects of subchronic ethanol consumption on DNA and lipids by measuring biomarkers 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and malondialdehyde (MDA), respectively. Physiological responses of pigs (n = 4) administered ethanol in drinking water for 39 days were compared with those of water-fed pigs (n = 4). Alcoholisation resulted in serum ethanol concentration of 1.90 g L(-1) and in a moderate but significant increase in alanine aminotransferase activity, an index of liver injury. However, between the alcoholised and control groups there were no significant differences in the levels of 8-oxodG (8-oxodG per 10(6) 2'deoxyguanosine) from leucocytes (2.52 ± 0.42 Vs 2.39 ± 0.34) or from target organs, liver, cardia and oesophagus. Serum MDA levels were also similar in ethanol-fed pigs (0.33 ± 0.04 μM) and controls (0.28 ± 0.03 μM). Interestingly, levels of 8-oxodG in cardia were positively correlated with those in oesophagus (Spearman correlation coefficient R = 1, P < 0.0001). Our results suggest that alcohol consumption may not cause oxidative damage to DNA and lipids as measured by 8-oxodG and MDA, respectively. The duration of alcoholisation and the potential alcohol-induced nutritional deficiency may be critical determinants of ethanol toxicity. Relevant biomarkers, such as factors involved in sensitization to ethanol-induced oxidative stress are required to better elucidate the relationship between alcohol consumption, oxidative stress and carcinogenesis.
authors.affiliation: ANSES, Laboratoire de Fougères, BP 90203, 35302 Fougères cedex, France.
authors.forename: F
authors.initials: F
authors.lastname: Petitpas
authors.forename: F
authors.initials: F
authors.lastname: Sichel
authors.forename: B
authors.initials: B
authors.lastname: Hébert
authors.forename: S
authors.initials: S
authors.lastname: Lagadu
authors.forename: M
authors.initials: M
authors.lastname: Beljean
authors.forename: D
authors.initials: D
authors.lastname: Pottier
authors.forename: M
authors.initials: M
authors.lastname: Laurentie
authors.forename: V
authors.initials: V
authors.lastname: Prevost
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000410:Alanine Transaminase / Q000097:blood; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000097:blood; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects*; D013552:Swine
chemical_list: D015415:Biomarkers; D000431:Ethanol; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002097:C-Reactive Protein; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003849:Deoxyguanosine