title: Molecular cloning, sequencing, and expression of the outer membrane protein P2 gene of Haemophilus parasuis.
abstract: Haemophilus parasuis is the etiological agent of Glässer's disease characterized by fibrinous polyserositis, polyarthritis, and meningitis in young pigs. But it is difficult to develop universal serological diagnostic tools and effective vaccines against this disease because of the serovar diversity of the isolates. In this study, enterobacterial repetitive intergenic consensus-polymerase chain reaction, were performed to investigate the gene profile of 111 isolates of H. parasuis from China. And a specific common gene of H. parasuis was cloned and identified as the outer-membrane protein (OMP) P2 gene. Sequencing results of OMP P2 genes of 22 isolates showed that they had high homology and could be divided into 2 genetic types. Moreover, the OMPP2 protein was expressed in Escherichia coli expressing system. And the purified recombinant protein provided partial protection against H. parasuis infection in mice. It suggested the OMP P2 was an immunogenic protein and had great potential to serve as a vaccine and diagnostic antigen.
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.forename: Jun-xing
authors.initials: JX
authors.lastname: Li
authors.forename: Guo-long
authors.initials: GL
authors.lastname: Zhang
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Song
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Li
authors.forename: Xian-wei
authors.initials: XW
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D014644:Genetic Variation; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044137:Haemophilus parasuis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial