title: DNA sexing of Nipponia nippon by duplex polymerase chain reaction.
abstract: The aim of this study was to develop a rapid, simple, sensitive, and accurate duplex polymerase chain reaction (PCR) to sex Nipponia nippon, a monomorphic bird. Amplification by duplex PCR of a sex-related gene on the female chromosome and the 12S rRNA gene yielded good results using genomic DNA extracted from a feather follicle or the membranes of eggshell samples. To simplify the DNA extraction procedure, a simple boiling method was used. Our simple boiling DNA extraction method produced similar PCR amplification results as when using DNA extracted using TRIzol. Sex determination in the endangered Nipponia nippon is of crucial value to breeding programs. The duplex PCR protocol that we developed provides a simple sex identification method that is based on amplification of a sex-related gene, and we anticipate that it will facilitate effective conservation and management of Nipponia nippon.
authors.affiliation: Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, Korea.
authors.forename: Jie Yeun
authors.initials: JY
authors.lastname: Park
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Seon A
authors.initials: SA
authors.lastname: Choi
authors.forename: Keun Jung
authors.initials: KJ
authors.lastname: Kim
authors.forename: Il Beum
authors.initials: IB
authors.lastname: Lee
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
mesh_terms: D000818:Animals; D001717:Birds / Q000235:genetics* / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics; D004247:DNA / Q000235:genetics*; D005260:Female; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*
chemical_list: D004247:DNA