title: Expression of Semaphorin 4F in neurons and brain oligodendrocytes and the regulation of oligodendrocyte precursor migration in the optic nerve.
abstract: Semaphorins are secreted or membrane-anchored proteins that play critical roles in neural development and adult brain plasticity. Sema4F is a transmembrane semaphorin found on glutamatergic synapses, in which it is attached to the PSD-95-scaffolding protein. Here we further examined the expression of Sema4F by raising specific antibodies. We show that Sema4F protein is widely expressed by neurons during neural development and in the adult brain. We also demonstrate a preferential localization of this protein in postsynaptic dendrites. Moreover, Sema4F is expressed not only by neurons but also by oligodendrocyte precursors in the optic nerve and along the migratory pathways of oligodendroglial cells, and also by subsets of postnatal oligodendroglial cells in the brain. Finally, in vitro experiments demonstrate that endogenous Sema4F expressed by brain cells of oligodendroglial lineage regulates the outgrowth migration of oligodendrocyte precursors and promotes their differentiation. The present data extend our knowledge about the expression of Sema4F and uncover a novel function in the control of oligodendrocyte precursor migration in the developing brain.
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac, 10, 08028, Barcelona, Spain; CIBERNED (ISCIII), Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Armendáriz
authors.affiliation: Institute for Bioengineering of Catalonia, IBEC-PCB, Baldiri Reixac, 9, 08028, Barcelona, Spain; Developmental Neurobiology Lab., Hospital Nacional de Parapléjicos, 45005 Toledo, Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Bribian
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac, 10, 08028, Barcelona, Spain; CIBERNED (ISCIII), Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Pérez-Martínez
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac, 10, 08028, Barcelona, Spain; CIBERNED (ISCIII), Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Martínez
authors.affiliation: Developmental Neurobiology Lab., Hospital Nacional de Parapléjicos, 45005 Toledo, Spain; Instituto Cajal, CSIC, Dr. Arce, 37, 28002, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de Castro
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac, 10, 08028, Barcelona, Spain; CIBERNED (ISCIII), Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Soriano
authors.affiliation: Institute for Research in Biomedicine (IRB Barcelona), Baldiri Reixac, 10, 08028, Barcelona, Spain; CIBERNED (ISCIII), Spain; Cell Biology Department, Faculty of Biology, Universitat de Barcelona, Diagonal, 645, 08028, Barcelona, Spain. Electronic address: ferran.burgaya@irbbarcelona.org.
authors.forename: Ferran
authors.initials: F
authors.lastname: Burgaya
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000648:ultrastructure; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D009900:Optic Nerve / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C485552:Sema4f protein, mouse