title: EGFR and KRAS mutations detection on lung cancer liquid-based cytology: a pilot study.
abstract: In advanced non-small-cell lung carcinomas epidermal growth factor receptor (EGFR) and KRAS testing is often performed on cytology. Liquid-based cytology (LBC), which eliminates the need for slide preparation by clinicians, may be very useful. In 42 LBC DNA was extracted twice. One sample was obtained directly from CytoLyt solution, whereas the other DNA sample was derived after smear preparation and laser capture microdissection (LCM) of Papanicolaou-stained cells. EGFR and KRAS mutational analyses were performed by direct sequencing. On CytoLyt-derived DNA four EGFR (9%) and five KRAS (12%) gene mutations were found. When direct sequencing was performed after LCM, the rate of cases that displayed either EGFR or KRAS mutations increased from 21% to 40%. Although time-consuming, LCM makes direct sequencing highly sensitive even on LBC preparations containing only a few cells.
authors.affiliation: Scienze Biomorfologiche e Funzionali, University Federico II of Naples, Naples, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Malapelle
authors.forename: Nicla
authors.initials: N
authors.lastname: de Rosa
authors.forename: Danilo
authors.initials: D
authors.lastname: Rocco
authors.forename: Claudio
authors.initials: C
authors.lastname: Bellevicine
authors.forename: Carlo
authors.initials: C
authors.lastname: Crispino
authors.forename: Alfonso
authors.initials: A
authors.lastname: Illiano
authors.forename: Franco Vito
authors.initials: FV
authors.lastname: Piantedosi
authors.forename: Oscar
authors.initials: O
authors.lastname: Nappi
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D007558:Italy; D061346:Laser Capture Microdissection; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods; D018631:ras Proteins / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C117307:KRAS protein, human; D011518:Proto-Oncogene Proteins; C512478:EGFR protein, human; D066246:ErbB Receptors; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins