title: Chrysin protects mice from Staphylococcus aureus pneumonia.
abstract: OBJECTIVE
  To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model.
  
  
  RESULTS
  Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The efficacy of chrysin against human alveolar epithelial cell injury by α-haemolysin was tested using live/dead staining or by measuring lactate dehydrogenase activity. Furthermore, we determined the protective effect of chrysin against Staph. aureus pneumonia through histopathology experiments in a mouse model. The production of α-haemolysin by Staph. aureus was inhibited when presented with an increasing subinhibitory concentration of chrysin in vitro. Consistent with this result, chrysin prevented α-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia.
  
  
  CONCLUSIONS
  Chrysin is a potent inhibitor of α-haemolysin expression by Staph. aureus, and it conferred a significant degree of protection against Staph. aureus pneumonia.
  
  
  CONCLUSIONS
  The chrysin-mediated inhibition of α-haemolysin production and protection against Staph. aureus pneumonia may offer a new strategy in combating pathogen infections.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Qiu
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Luo
authors.forename: X
authors.initials: X
authors.lastname: Dai
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Leng
authors.forename: X
authors.initials: X
authors.lastname: Niu
authors.forename: S
authors.initials: S
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Deng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006461:Hemolysis; D006801:Humans; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008826:Microbial Sensitivity Tests; D011023:Pneumonia, Staphylococcal / Q000517:prevention & control*; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
chemical_list: D005419:Flavonoids; D006460:Hemolysin Proteins; C043561:chrysin