title: Oxidative changes of lipids monitored by MALDI MS.
abstract: Oxidation processes of lipids are of paramount interest from many viewpoints. For instance, oxidation processes are highly important under in vivo conditions because molecules with regulatory functions are generated by oxidation of lipids or free fatty acids. Additionally, many inflammatory diseases are accompanied by lipid oxidation and, therefore, oxidation products are also useful disease (bio)markers. Thus, there is also considerable interest in methods of (oxidized) lipid analysis. Nowadays, soft ionization mass spectrometric (MS) methods are regularly used to study oxidative lipid modifications due to their high sensitivities and the extreme mass resolution. Although electrospray ionization (ESI) MS is so far most popular, applications of matrix-assisted laser desorption and ionization (MALDI) MS are increasing. This review aims to summarize the so far available data on MALDI analyses of oxidized lipids. In addition to model systems, special attention will be paid to the monitoring of oxidized lipids under in vivo conditions, particularly the oxidation of (human) lipoproteins. It is not the aim of this review to praise MALDI as the "best" method but to provide a critical survey of the advantages and drawbacks of this method.
authors.affiliation: University of Leipzig, Faculty of Medicine, Institute of Medical Physics and Biophysics, Härtelstrasse16/18, Leipzig, Germany.
authors.forename: Beate
authors.initials: B
authors.lastname: Fuchs
authors.forename: Kristin
authors.initials: K
authors.lastname: Bresler
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schiller
mesh_terms: D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D010084:Oxidation-Reduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008055:Lipids