title: Overexpression of megsin induces mesangial cell proliferation and excretion of type IV collagen in vitro.
abstract: Over-expression of megsin is associated with mesangial cell (MC) proliferation and extracellular matrix (ECM) accumulation. The underlying pathogenesis is unknown. This study demonstrate that over-expression of megsin induced incorporation of [(3)H]thymidine in MCs and PDGF-BB, TGF-β1 upregulation. Concentrations of PDGF-BB, TGF-β1 and type IV collagen in the culture medium of MCs transfected with megsin were higher than controls. Anti-PDGF-BB suppressed incorporation of [(3)H]thymidine in MCs transfected with megsin and mRNA expression of TGF-β1 in stable transformant MCs, suggesting that over-expression of megsin induces cell proliferation and ECM accumulation in MCs, upregulation of PDGF-BB and TGF-β1 is probably the main route involved in pathogenesis.
authors.affiliation: Department of Nephrology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, PR China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Xia
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.forename: Shuangxin
authors.initials: S
authors.lastname: Liu
authors.forename: Yuanhan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xinling
authors.initials: X
authors.lastname: Liang
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D001483:Base Sequence; D000077214:Becaplermin; D002460:Cell Line; D049109:Cell Proliferation; D024141:Collagen Type IV / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics; D050527:Mesangial Cells / Q000276:immunology / Q000378:metabolism* / Q000473:pathology*; D010982:Platelet-Derived Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015843:Serpins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D024141:Collagen Type IV; D016207:Cytokines; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; C491937:Serpinb7 protein, rat; D015843:Serpins; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin