title: Proteomic analysis of four Brazilian MON810 maize varieties and their four non-genetically-modified isogenic varieties.
abstract: Profiling techniques have been suggested as a nontargeted approach to detect unintended effects in genetically modified (GM) plants. Seedlings from eight Brazilian maize varieties, four MON810 GM varieties and four non-GM isogenic varieties, were grown under controlled environmental conditions. Physiological parameters (aerial part weight, main leaf length, and chlorophyll and total protein contents) were compared, and some differences were observed. Nevertheless, these differences were not constant among all GM and non-GM counterparts. Leaf proteomic profiles were analyzed using two-dimensional gel electrophoresis (2DE) coupled to mass spectrometry, using six 2DE gels per variety. The comparison between MON810 and its counterpart was limited to qualitative differences of fully reproducible protein spot patterns. Twelve exclusive proteins were observed in two of four maize variety pairs; all of these leaf proteins were variety specific. In this study, MON810 leaf proteomes of four varieties were similar to non-GM counterpart leaf proteomes.
authors.affiliation: Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga 1346, 88034-001 Florianópolis-SC, Brazil.
authors.forename: Geisi M
authors.initials: GM
authors.lastname: Balsamo
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Cangahuala-Inocente
authors.forename: Jean B
authors.initials: JB
authors.lastname: Bertoldo
authors.forename: Hernán
authors.initials: H
authors.lastname: Terenzi
authors.forename: Ana C M
authors.initials: AC
authors.lastname: Arisi
mesh_terms: D001938:Brazil; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins