title: Identification, structure and function of a novel tetraspaninhomologue from Spirometra erinaceieuropaei.
abstract: OBJECTIVE
  To identify a full length c DNA sequence of a novel tetraspanin (TSP) homologue from Spirometra erinaceieuropaei and to predict the structure and function of its encoding protein using bioinformatics methods.
  
  
  METHODS
  Using the NCBI, EMBI, Expasy and other online sites, the open reading frame (ORF), conserved domain, physical and chemical parameters, signal peptide, transmembrane domain, epitope, topological structures of the protein sequences were predicted. And Vector NTI software was used for multiple sequence alignment and phylogenetic tree construction.
  
  
  RESULTS
  The target sequence was 1132 bp length with a 681 bpbiggest ORF encoding 226 amino acids protein with typical TSP conserved domain. It was confirmed as full length c DNA of TSP16 from Spirometra erinaceieuropaei and named as SeTSP16 (GenBank accession number: JF728872). The predicted molecular weight and isoelectric point of the deduced protein were 24 750.5 Da and 7.88 Da, respectively. Compared with TSP16s from Schistosoma japonicum and Schistosoma mansoni, it showed similarity of 59% and 59%, respectively. SeTSP16 contained four transmembrane domains (TM1-4), intracellular N and C-termini, one short small extracellular loop and one large extracellular loop. Four major epitopes that were significant different from the corresponding epitope regions of TSP16 from Schistosoma mansoni and Schistosoma japonicum were predicted.
  
  
  CONCLUSIONS
  The full length c DNA sequences of SeTSP16 are identified. It encodes a transmembrane protein which might be an ideal diagnosis antigen and target molecule for antiparasitic drugs.
authors.affiliation: Department of Clinical Laboratoy, Hainan Medical College, Haikou, PR China.
authors.forename: Fu
authors.initials: F
authors.lastname: Qiongyao
authors.forename: Lu
authors.initials: L
authors.lastname: Yajun
authors.forename: Rao
authors.initials: R
authors.lastname: Langyu
authors.forename: Chen
authors.initials: C
authors.lastname: Jinglong
authors.forename: Wu
authors.initials: W
authors.lastname: Qiang
authors.forename: Cai
authors.initials: C
authors.lastname: Qunfang
authors.forename: Li
authors.initials: L
authors.lastname: Lihua
authors.forename: Wu
authors.initials: W
authors.lastname: Lixian
authors.forename: Lu
authors.initials: L
authors.lastname: Gang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D016415:Sequence Alignment; D013146:Spirometra / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D059470:Tetraspanins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D015801:Helminth Proteins; D059470:Tetraspanins