title: Tiam1, overexpressed in most malignancies, is a novel tumor biomarker.
abstract: T lymphoma invasion and metastasis 1 (Tiam1) is a guanine nucleotide exchange factor (GNEF) family member, and is considered to be involved in many important cellular processes and oncogenesis. In this study, we investigated Tiam1 expression differences between normal tissue and malignant tissue using tissue microarray (TMA), and further studied the Tiam1 mRNA and protein level in 9 hepatoma lines. Forty-nine tumor tissue and 47 normal tissue samples were detected via TMA by immunohistochemistry with polyclonal antibody. Tiam1 expression in 9 human hepatoma cell lines, namely Huh-7, HepG2, Hep3B, SMMC-7721, QGy-7701, QGy-7703, BEL-7402, BEL-7404 and BEL-7405, and 1 normal primary human hepatocyte, HL-7702, was compared by means of fluorescence quantitative PCR, immunocytochemistry assay and Western blotting. We found that Tiam1 was significantly expressed in various malignancies. Tiam1 mRNA and protein levels were significantly elevated in the 9 human hepatoma cell lines compared to the normal primary human hepatocyte. Our results suggest that Tiam1 overexpression in malignant neoplasms could be a novel effective supplementary biomarker for tumors, including hepatocellular carcinoma.
authors.affiliation: General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510515, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Feiye
authors.initials: F
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Ruan
authors.forename: Jian
authors.initials: J
authors.lastname: Guan
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Xianghua
authors.initials: X
authors.lastname: Ye
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Gong
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liang
authors.forename: Rongcheng
authors.initials: R
authors.lastname: Luo
authors.forename: Longhua
authors.initials: L
authors.lastname: Chen
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000378:metabolism; D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000175:diagnosis / Q000378:metabolism; D046228:Microarray Analysis; D012333:RNA, Messenger / Q000378:metabolism; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1
chemical_list: D014408:Biomarkers, Tumor; D020662:Guanine Nucleotide Exchange Factors; D012333:RNA, Messenger; D000075684:T-Lymphoma Invasion and Metastasis-inducing Protein 1; C087334:TIAM1 protein, human