title: Characterization of melanocortin receptors.
abstract: This unit describes a Scintillation Proximity Assay (SPA) for the measurement of ligand binding to melanocortin receptors (MCRs) using membranes prepared from cell lines stably expressing recombinant MCRs. It provides a facile method for determining the affinity of compounds at MC1R, MC3R, MC4R, or MC5R.
authors.affiliation: GlaxoSmithKline, Research Triangle Park, North Carolina, USA
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Goetz
authors.forename: Diane M
authors.initials: DM
authors.lastname: Ignar
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D007457:Iodine Radioisotopes; D044102:Receptor, Melanocortin, Type 1 / Q000032:analysis / Q000378:metabolism; D044104:Receptor, Melanocortin, Type 3 / Q000032:analysis / Q000378:metabolism; D044105:Receptor, Melanocortin, Type 4 / Q000032:analysis / Q000378:metabolism; D044101:Receptors, Melanocortin / Q000032:analysis* / Q000378:metabolism; D000521:alpha-MSH / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D007457:Iodine Radioisotopes; D044102:Receptor, Melanocortin, Type 1; D044104:Receptor, Melanocortin, Type 3; D044105:Receptor, Melanocortin, Type 4; D044101:Receptors, Melanocortin; C086971:melanocortin 5 receptor; D000521:alpha-MSH; C027756:MSH, 4-Nle-7-Phe-alpha-