title: Mouse mammary tumor virus-like gene sequences are present in lung patient specimens.
abstract: BACKGROUND
  Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like gene sequences in lung diseases including carcinomas specimens from a Mexican population. This study was based on our previous study reporting that the INER51 lung cancer cell line, from a pleural effusion of a Mexican patient, contains MMTV-like env gene sequences.
  
  
  RESULTS
  The MMTV-like env gene sequences have been detected in three out of 18 specimens studied, by PCR using a specific set of MMTV-like primers. The three identified MMTV-like gene sequences, which were assigned as INER6, HZ101, and HZ14, were 99%, 98%, and 97% homologous, respectively, as compared to GenBank sequence accession number AY161347. The INER6 and HZ-101 samples were isolated from lung cancer specimens, and the HZ-14 was isolated from an acute inflammatory lung infiltrate sample. Two of the env sequences exhibited disruption of the reading frame due to mutations.
  
  
  CONCLUSIONS
  In summary, we identified the presence of MMTV-like gene sequences in 2 out of 11 (18%) of the lung carcinomas and 1 out of 7 (14%) of acute inflamatory lung infiltrate specimens studied of a Mexican Population.
authors.affiliation: Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ave, Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, 66451, Mexico. laura.trejoav@uanl.edu.mx
authors.forename: Laura M
authors.initials: LM
authors.lastname: Trejo-Avila
authors.forename: Pablo
authors.initials: P
authors.lastname: Zapata-Benavides
authors.forename: Raúl
authors.initials: R
authors.lastname: Barrera-Rodríguez
authors.forename: Isaías
authors.initials: I
authors.lastname: Badillo-Almaráz
authors.forename: Santiago
authors.initials: S
authors.lastname: Saavedra-Alonso
authors.forename: Diana E
authors.initials: DE
authors.lastname: Zamora-Avila
authors.forename: Karla
authors.initials: K
authors.lastname: Morán-Santibañez
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Garza-Sáenz
authors.forename: Reyes
authors.initials: R
authors.lastname: Tamez-Guerra
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodríguez-Padilla
mesh_terms: D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D002277:Carcinoma / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics; D030541:Databases, Genetic; D005260:Female; D015752:Genes, env; D005820:Genetic Testing; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000302:isolation & purification; D008800:Mexico / Q000453:epidemiology; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016066:Pleural Effusion, Malignant / Q000737:chemistry; D011014:Pneumonia / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D016133:Polymerase Chain Reaction; D012192:Retroviridae Infections / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000235:genetics / Q000473:pathology / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral