title: Screening and determination for potential α-glucosidase inhibitors from leaves of Acanthopanax senticosus harms by using UF-LC/MS and ESI-MS(n).
abstract: BACKGROUND
  Acanthopanax senticosus Harms is a typical Chinese herb with flavonoids existing in all parts of the plant but with the largest content in leaves. However, leaves have been neglected in past research. To investigate the potential use of leaves of A. senticosus Harms for discovering lead compounds to treat type 2 diabetes, the herb leaves were selected for screening the potential of α-glucosidase inhibitors.
  
  
  OBJECTIVE
  To screen for candidates of α-glucosidase inhibitors from leaves of A. senticosus Harms and evaluate the structure-activity relationship of the α-glucosidase inhibitors.
  
  
  METHODS
  Ultrafiltration liquid chromatography/mass spectrometry (UF-LC/MS) assay was developed for screening candidates of α-glucosidase inhibitors from leaves of A. senticosus Harms. The interesting compounds were identified by using reversed-phase high performance liquid chromatography with diode array detector and electrospray ionisation multiple-stage tandem mass spectrometry (RP-HPLC-DAD-ESI-MS(n) ), and confirmed by using electrospray ionisation Fourier transform ion cyclotron resonance multiple-stage tandem mass spectrometry (ESI-FT-ICR-MS(n)). Furthermore, the α-glucosidase inhibitory activity of the compounds detected was estimated using in vitro assays.
  
  
  RESULTS
  Eight compounds that might bind to α-glucosidase were screened and seven of them were identified successfully. The α-glucosidase inhibitory activity of the related compounds in leaves of A. senticosus Harms was determined.
  
  
  CONCLUSIONS
  The results obtained provided new information for the discovery of potential α-glucosidase inhibitors and the potential anti-diabetic application of the leaves of A. senticosus Harms.
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R., China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Junpeng
authors.initials: J
authors.lastname: Xing
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.forename: Fengrui
authors.initials: F
authors.lastname: Song
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Pi
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Shuying
authors.initials: S
authors.lastname: Liu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D028001:Eleutherococcus / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification; D008024:Ligands; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry*; D011794:Quercetin / Q000737:chemistry / Q000302:isolation & purification; D012431:Rutin / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013329:Structure-Activity Relationship; D014462:Ultrafiltration / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D008024:Ligands; D012431:Rutin; D011794:Quercetin