title: Optimization of medium compositions favoring butanol and 1,3-propanediol production from glycerol by Clostridium pasteurianum.
abstract: Medium compositions favoring butanol and 1,3-propanediol (1,3-PDO) production from glycerol by Clostridium pasteurianum DSM525 were investigated using statistical experimental designs. Medium components affecting butanol and 1,3-PDO production were screened using a fractional factorial experimental design. Among the six tested variables (phosphate buffer, MnSO4·H2O, MgSO4·7H2O, FeSO4·7H2O, (NH4)2SO4, and yeast extract), FeSO4·7H2O, (NH4)2SO4, and yeast extract were found to be significant variables for further optimization of medium using a Box-Behnken design. Optimal butanol (0.98 g/L/h) and 1,3-PDO (1.19 g/L/h) productivities were predicted by the corresponding quadratic model for each product and the models were validated experimentally under optimized conditions. The optimal medium composition for butanol production was significantly different from that for 1,3-PDO production (0.06 vs. 0 g/L for FeSO4·7H2O, 7.35 vs. 0 g/L for (NH4)2SO4, and 5.08 vs. 8.0 g/L for yeast extract), suggesting that the product formation from glycerol by C. pasteurianum DSM525 can be controlled by changing medium compositions.
authors.affiliation: Clean Energy Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Chuloo
authors.initials: C
authors.lastname: Moon
authors.forename: Chang Hwan
authors.initials: CH
authors.lastname: Lee
authors.forename: Byoung-In
authors.initials: BI
authors.lastname: Sang
authors.forename: Youngsoon
authors.initials: Y
authors.lastname: Um
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000187:drug effects* / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D008954:Models, Biological; D011409:Propylene Glycols / Q000378:metabolism*; D012044:Regression Analysis; D015203:Reproducibility of Results
chemical_list: D000440:Butanols; D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D005990:Glycerol