title: Biomarkers of exposure and effect-interpretation in human risk assessment.
abstract: The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), 142 20 Prague, Czech Republic.
authors.forename: Radim J
authors.initials: RJ
authors.lastname: Sram
authors.forename: Blanka
authors.initials: B
authors.lastname: Binkova
authors.forename: Olena
authors.initials: O
authors.lastname: Beskid
authors.forename: Alena
authors.initials: A
authors.lastname: Milcova
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.forename: Andrea
authors.initials: A
authors.lastname: Rossnerova
authors.forename: Ivo
authors.initials: I
authors.lastname: Solansky
authors.forename: Jan
authors.initials: J
authors.lastname: Topinka