title: Modeling of influenza-specific CD8+ T cells during the primary response indicates that the spleen is a major source of effectors.
abstract: The biological parameters that determine the distribution of virus-specific CD8(+) T cells during influenza infection are not all directly measurable by experimental techniques but can be inferred through mathematical modeling. Mechanistic and semimechanistic ordinary differential equations were developed to describe the expansion, trafficking, and disappearance of activated virus-specific CD8(+) T cells in lymph nodes, spleens, and lungs of mice during primary influenza A infection. An intensive sampling of virus-specific CD8(+) T cells from these three compartments was used to inform the models. Rigorous statistical fitting of the models to the experimental data allowed estimation of important biological parameters. Although the draining lymph node is the first tissue in which Ag-specific CD8(+) T cells are detected, it was found that the spleen contributes the greatest number of effector CD8(+) T cells to the lung, with rates of expansion and migration that exceeded those of the draining lymph node. In addition, models that were based on the number and kinetics of professional APCs fit the data better than those based on viral load, suggesting that the immune response is limited by Ag presentation rather than the amount of virus. Modeling also suggests that loss of effector T cells from the lung is significant and time dependent, increasing toward the end of the acute response. Together, these efforts provide a better understanding of the primary CD8(+) T cell response to influenza infection, changing the view that the spleen plays a minor role in the primary immune response.
authors.affiliation: Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Hulin
authors.initials: H
authors.lastname: Wu
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
authors.forename: Hongyu
authors.initials: H
authors.lastname: Miao
authors.forename: Jeanne
authors.initials: J
authors.lastname: Holden-Wiltse
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Mosmann
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Livingstone
authors.forename: Gabrielle T
authors.initials: GT
authors.lastname: Belz
authors.forename: Alan S
authors.initials: AS
authors.lastname: Perelson
authors.forename: Martin S
authors.initials: MS
authors.lastname: Zand
authors.forename: David J
authors.initials: DJ
authors.lastname: Topham
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology / Q000821:virology*; D002465:Cell Movement / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology / Q000821:virology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018448:Models, Immunological; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000473:pathology / Q000821:virology; D013154:Spleen / Q000276:immunology* / Q000473:pathology / Q000821:virology*