title: Turnover of nonessential fatty acids in cardiolipin from the rat heart.
abstract: Cardiolipin (CL) is a unique phospholipid (PL) found in the mitochondria of mammalian cells. CL remodeling is accompanied by turnover of its fatty acid acyl groups. Abnormalities in CL remodeling have been found in Barth's syndrome, diabetes, and obesity. The objective of this study was to determine nonessential fatty acid turnover in CL and phosphatidylethanolamine (PE) in the rat heart in vivo. Sprague-Dawley rats were fed either a regular chow or a high-fat diet for 15 weeks, and consumed 6% deuterium-enriched drinking water as a tracer for 14 days. CL and PE were extracted from cardiac tissue and isolated by TLC. Fatty acids from CL, PE, and plasma were analyzed by GC/MS for deuterium incorporation. Results showed oleate and vaccenate turnover were the highest in CL whereas palmitate and stearate turnover were low. Among the nonessential fatty acids in PE, turnover of stearate and vaccenate were the highest. The high turnover rate in vaccenate was unexpected, because vaccenate previously had no known metabolic or physiologic function. In conclusion, the similarly high turnover rates of both oleate and vaccenate readily suggest that remodeling is an important functional aspect of PL metabolism in CL.
authors.affiliation: LA Biomedical Research Institute, Torrance, CA.
authors.forename: Paulin N
authors.initials: PN
authors.lastname: Wahjudi
authors.affiliation: LA Biomedical Research Institute, Torrance, CA; Division of Endocrinology, Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: K Yee
authors.affiliation: LA Biomedical Research Institute, Torrance, CA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Martinez
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA.
authors.forename: Michael
authors.initials: M
authors.lastname: Teitell
authors.affiliation: LA Biomedical Research Institute, Torrance, CA; Division of Endocrinology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA and.
authors.forename: Liana
authors.initials: L
authors.lastname: Nikolaenko
authors.affiliation: LA Biomedical Research Institute, Torrance, CA; Division of Endocrinology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA and.
authors.forename: Ronald
authors.initials: R
authors.lastname: Swerdloff
authors.affiliation: LA Biomedical Research Institute, Torrance, CA; Division of Endocrinology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA and.
authors.forename: Christina
authors.initials: C
authors.lastname: Wang
authors.affiliation: LA Biomedical Research Institute, Torrance, CA; Division of Endocrinology, Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA. Electronic address: lee@labiomed.org.
authors.forename: W N Paul
authors.initials: WNP
authors.lastname: Lee
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D002308:Cardiolipins / Q000737:chemistry* / Q000378:metabolism*; D003903:Deuterium / Q000737:chemistry; D059305:Diet, High-Fat; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000085:Acetates; D002308:Cardiolipins; D005230:Fatty Acids, Nonesterified; D010714:Phosphatidylethanolamines; C483858:phosphatidylethanolamine; D003903:Deuterium