title: An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells.
abstract: Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K(D) of 77nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer.
authors.affiliation: Molecular Imaging & Therapy Branch, Division of Convergence Technology, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 410-769, Republic of Korea.
authors.forename: Yun-Hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Ho Jin
authors.initials: HJ
authors.lastname: Sung
authors.forename: Sukyoung
authors.initials: S
authors.lastname: Kim
authors.forename: Eun-Ok
authors.initials: EO
authors.lastname: Kim
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Kyungho
authors.initials: K
authors.lastname: Choi
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Jung
authors.forename: Yangsoon
authors.initials: Y
authors.lastname: Lee
authors.forename: Sang Seok
authors.initials: SS
authors.lastname: Koh
authors.forename: Sue Goo
authors.initials: SG
authors.lastname: Rhee
authors.forename: Kyun
authors.initials: K
authors.lastname: Heo
authors.forename: In-Hoo
authors.initials: IH
authors.lastname: Kim
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D013936:Thymidine / Q000031:analogs & derivatives / Q000737:chemistry; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D052157:Aptamers, Nucleotide; D036341:Intercellular Signaling Peptides and Proteins; D037102:Lectins; C517340:ZG16B protein, human; C068636:2'-fluorothymidine; D013936:Thymidine