title: Functional screening in the melanophore bioassay.
abstract: The melanophore bioassay is a robust, sensitive, and versatile procedure for screening G protein-coupled receptors in a variety of formats. Because melanophores contain a wide variety of G proteins, they can be employed as a sensitive, real-time response system for studying transfected receptors and for defining equilibria for drug effects. This assay can be run in 96-well microtiter plates or in open-lawn 1536 format, and can yield conventional agonist-antagonist as well as constitutive assays.
authors.affiliation: GlaxoSmithKline, Research Triangle Park, North Carolina, USA.
authors.forename: Channa
authors.initials: C
authors.lastname: Jayawickreme
authors.forename: Howard
authors.initials: H
authors.lastname: Sauls
authors.forename: Chris
authors.initials: C
authors.lastname: Watson
authors.forename: David
authors.initials: D
authors.lastname: Moncol
authors.forename: Thomas
authors.initials: T
authors.lastname: Rimele
authors.forename: Terry
authors.initials: T
authors.lastname: Kenakin
mesh_terms: D000818:Animals; D002460:Cell Line; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008547:Melanophores / Q000187:drug effects* / Q000502:physiology; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D043562:Receptors, G-Protein-Coupled