title: Electrophysiological analysis of ATP-sensitive potassium channels in mammalian cells and Xenopus oocytes.
abstract: This unit describes general methodologies for the characterization of ATP-sensitive K+ channels and the study of ligand-channel interactions in native tissues and clonal cell lines by electrophysiological techniques. Detailed protocols on how to establish patch-clamp single-channel and whole-cell current recording are presented. Two-electrode voltage clamp techniques for studying ATP-sensitive K+ channels expressed in Xenopus oocytes are also included.
authors.affiliation: Abbott Laboratories, Abbott Park, Illinois, USA.
authors.forename: Char-Chang
authors.initials: CC
authors.lastname: Shieh
authors.forename: Murali
authors.initials: M
authors.lastname: Gopalakrishnan
mesh_terms: D000818:Animals; D004594:Electrophysiology / Q000379:methods*; D005260:Female; D006801:Humans; D054086:KATP Channels / Q000187:drug effects / Q000502:physiology*; D009865:Oocytes; D018408:Patch-Clamp Techniques; D014981:Xenopus
chemical_list: D054086:KATP Channels