title: Identification and subsequent removal of an interference by FAIMS in the bioanalysis of dianicline in animal plasma.
abstract: BACKGROUND
  The determination of pharmacokinetic parameters requires accurate and reliable bioanalytical methods. Even using highly selective MS/MS, interferences can occur. This paper describes the source of some of these interferences with an example discussed involving the problem of a ketamine interference in a plasma assay.
  
  
  RESULTS
  The introduction of field asymmetric waveform ion mobility spectrometry (FAIMS) removed the interference, enhanced signal-to-background and met GLP acceptance criteria. Relative to the non-FAIMS method, assay calibration characteristics were improved. The FAIMS source gave optimal performance following the introduction of a split in order to reduce the inlet flow to approximately 0.4 ml/min.
  
  
  CONCLUSIONS
  The introduction of ion-mobility separation into a bioanalytical LC-MS/MS method can remove unexpected isobaric interferences without the need to redevelop the chromatography.
authors.affiliation: Bioanalytical Services, Covance Laboratories, Willowburn Avenue, Alnwick, Northumberland, UK. michael.blackburn@covance.com
authors.forename: Michael A
authors.initials: MA
authors.lastname: Blackburn
authors.forename: James
authors.initials: J
authors.lastname: Kapron
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mackie
authors.forename: John
authors.initials: J
authors.lastname: Firth
authors.forename: Mark
authors.initials: M
authors.lastname: Harrison
authors.forename: Stuart
authors.initials: S
authors.lastname: McDougall
mesh_terms: D000818:Animals; D001381:Azepines / Q000097:blood* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006576:Heterocyclic Compounds, 4 or More Rings / Q000097:blood* / Q000737:chemistry; D007649:Ketamine / Q000737:chemistry; D008251:Macaca; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards
chemical_list: D001381:Azepines; D006576:Heterocyclic Compounds, 4 or More Rings; D007649:Ketamine; C475773:dianicline