title: [Construction of eukaryotic expression vector encoding ACRBP and its expression in hepatocarcinoma cells].
abstract: OBJECTIVE
  To construct the eukaryotic expression vector pEGFP-N1/ACRBP and stably express ACRBP in human hepatocarcinoma cells,  providing functional clues for ACRBP.
  
  
  METHODS
  A recombinant plasmid pMAL-C2/ACRBP was used as a template to amplify ACRBP cDNA. The PCR product was ligated into an eukaryotic expression vector pEGFP-N1 to construct a recombinant plasmid pEGFP-N1/ACRBP. Then the pEGFP-N1/ACRBP was transfected by Fugene HD into ACRBP-negative HepG2 cells. The stably transfected clones were selected by G418. RT-PCR and immunohistochemistry were used to detect the expression of ACRBP in HepG2 cells.
  
  
  RESULTS
  The eukaryotic expression vector pEGFP-N1/ACRBP was constructed and confirmed by sequencing. The stably transfected HepG2 cells expressed ACRBP.
  
  
  CONCLUSIONS
  The eukaryotic expression vector pEGFP-N1/ACRBP has been successfully constructed and transfected into HepG2 cells,  resulting in stable expression of ACRBP.
authors.affiliation: Department of Histology and Embryology, Guangxi Medical University, Nanning, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Luo
authors.forename: Xiang
authors.initials: X
authors.lastname: Yun
authors.forename: Yong-da
authors.initials: YD
authors.lastname: Lin
authors.forename: Shao-wen
authors.initials: SW
authors.lastname: Xiao
authors.forename: Guang-hua
authors.initials: GH
authors.lastname: Yan
authors.forename: Shao-jian
authors.initials: SJ
authors.lastname: He
authors.forename: Shu-jia
authors.initials: SJ
authors.lastname: He
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.forename: Xiao-xun
authors.initials: XX
authors.lastname: Xie
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors / Q000235:genetics*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
chemical_list: C485651:ACRBP protein, human; D002352:Carrier Proteins; D012333:RNA, Messenger