title: miR-191 down-regulation plays a role in thyroid follicular tumors through CDK6 targeting.
abstract: BACKGROUND
  Well-differentiated thyroid carcinomas include papillary (PTC) and follicular (FTC) carcinomas. FTC is usually a more aggressive form of cancer than the more common papillary type. miR-191 expression is frequently altered in several neoplasias, being up-regulated in some cases, such as pancreatic carcinomas, and down-regulated in other carcinomas, such as melanomas.
  
  
  OBJECTIVE
  The objective was to evaluate the expression and the role of miR-191 in thyroid carcinogenesis.
  
  
  METHODS
  The expression of miR-191 was analyzed in tissues from patients with follicular adenoma (n = 24), FTC (n = 24), PTC (n = 15), anaplastic thyroid carcinoma (n = 8), and the follicular variant of PTC (n = 6) compared with normal thyroid tissues by quantitative RT-PCR. miR-191 expression was restored in the follicular thyroid cell line WRO, and the effects on cell proliferation, migration, and target expression were evaluated.
  
  
  RESULTS
  miR-191 is down-regulated in follicular adenoma, FTC, and follicular variant of PTC. We identified CDK6, a serine-threonine kinase involved in the control of cell cycle progression, as a novel target of miR-191. Restoration of miR-191 expression in WRO cells reduces cell growth and migration rate on vitronectin. CDK6 overexpression, correlated with miR-191 down-regulation, was found in follicular adenoma and FTC, suggesting a role of miR-191 down-regulation in the generation of these neoplasias.
  
  
  CONCLUSIONS
  Our results suggest that miR-191 down-regulation plays a role in thyroid neoplasias of the follicular histotype, likely by targeting CDK6.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare, Istituto di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli Federico II, Napoli, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Colamaio
authors.forename: Eleonora
authors.initials: E
authors.lastname: Borbone
authors.forename: Lucia
authors.initials: L
authors.lastname: Russo
authors.forename: Mimma
authors.initials: M
authors.lastname: Bianco
authors.forename: Antonella
authors.initials: A
authors.lastname: Federico
authors.forename: Daniela
authors.initials: D
authors.lastname: Califano
authors.forename: Gennaro
authors.initials: G
authors.lastname: Chiappetta
authors.forename: Pierlorenzo
authors.initials: P
authors.lastname: Pallante
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Troncone
authors.forename: Sabrina
authors.initials: S
authors.lastname: Battista
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fusco
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000235:genetics / Q000378:metabolism*; D000236:Adenoma / Q000235:genetics / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D051361:Cyclin-Dependent Kinase 6 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013961:Thyroid Gland / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D051361:Cyclin-Dependent Kinase 6