title: Gene expression analysis of demineralized bone matrix-induced osteogenesis in human periosteal cells using cDNA array technology.
abstract: Demineralized  bone matrix (DBM) has been widely investigated as a biomaterial to promote new  bone formation and is utilized clinically for bone repair and regeneration. We  investigated gene expression patterns of osteogenic differentiation in human  periosteal (HPO) cells cultured with demineralized bone matrix, using cDNA  array technology. Osteogenic differentiation of HPO cells was determined using  alkaline phosphatase assay. In order to examine differential gene expression  during osteogenic differentiation, total RNA was isolated from HPO cells in the  absence or presence of DBM on day seven and analyzed using osteogenesis cDNA  gene array. The selected genes were verified using reverse transcriptase  (RT)-PCR analysis. Human periosteal cells differentiated along an osteogenic  lineage after treatment of DBM. The alkaline phosphatase activity assay showed  that HPO cells differentiated into an osteogenic lineage. Gene expression of  HPO cells treated with DBM for seven days was analyzed with cDNA array and  RT-PCR analyses. Expression of biglycan, TGF-β1, and TGF-βR1 was upregulated, whereas collagen14A1  expression was downregulated, as confirmed by RT-PCR. Human periosteal cells expressed  osteogenesis genes when treated with DBM. These findings provide new insight  into the capability of demineralized bone matrix to modulate the osteogenic  differentiation of human periosteal cells.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, King Chulalongkorn Memorial Hospital, Chulalongkorn University, Thai Red Cross Society, Bangkok, Thailand. Sittisak.H@chula.ac.th
authors.forename: S
authors.initials: S
authors.lastname: Honsawek
authors.forename: P
authors.initials: P
authors.lastname: Bumrungpanichthaworn
authors.forename: T
authors.initials: T
authors.lastname: Thitiset
authors.forename: L
authors.initials: L
authors.lastname: Wolfinbarger
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D058578:Biglycan / Q000096:biosynthesis; D018775:Bone Demineralization Technique; D001846:Bone Development / Q000235:genetics; D001857:Bone Matrix / Q000502:physiology*; D001861:Bone Regeneration; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D018076:DNA, Complementary; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010012:Osteogenesis / Q000235:genetics*; D010521:Periosteum / Q000166:cytology* / Q000378:metabolism; D012313:RNA / Q000032:analysis; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
chemical_list: D058578:Biglycan; D018076:DNA, Complementary; D053773:Transforming Growth Factor beta1; D012313:RNA; D003094:Collagen; D000469:Alkaline Phosphatase