title: Synthesis and properties of novel 2'-O-alkoxymethyl-modified nucleic acids.
abstract: Novel 2'-O-modified oligoribonucleotides with alkoxymethyl skeletons were synthesized, and their ability to hybridize complementary nucleic acids and their nuclease resistance were analyzed. The hybridization ability was improved by introducing electron-withdrawing groups and the increases in melting temperature (T(m) value) was particularly high for chlorine-substituted compounds. Nuclease resistance of these 2'-O-alkoxymethylated oligomers was lower than expected, but cyano substitution resulted in a higher nuclease resistance than 2'-O-methylation.
authors.affiliation: Department of Medical Genome Sciences, The University of Tokyo, Bioscience Building 702, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Arai
authors.forename: Naoki
authors.initials: N
authors.lastname: Uchiyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wada
mesh_terms: D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D009696:Nucleic Acids