title: Hepatoprotective activity of the Phyllanthus species on tert-butyl hydroperoxide (t-BH)-induced cytotoxicity in HepG2 cells.
abstract: BACKGROUND
  Phyllanthus (Euphorbiaceae) species have long been used in folk medicine to treat various pathological conditions including liver diseases. Some species of Phyllanthus were found to exhibit hepatoprotective activity against drugs or toxins and this property was majorly attributed to phyllanthin and hypophyllanthin. In this study, we examined the hepatoprotective activity of five different species of Phyllanthus, namely, Phyllanthus amarus, Phyllanthus fraternus, Phyllanthus maderaspatensis, Phyllanthus urinaria, and Phyllanthus Rotundifolius. The extracts were also evaluated for the presence of key phytoconstituents, phyllanthin and hypophyllanthin.
  
  
  METHODS
  The extracts were evaluated for hepatoprotective activity against tert-butyl hydroxide (t-BH)-induced cytotoxicity using human hepatocarcinoma cells (HepG2 cell line).
  
  
  RESULTS
  Only P. urinaria and P. maderaspatensis exhibited significant hepatoprotective activity as evident from increased cell viability. The HPLC profile revealed that except P. amarus, the other extracts did not contain phyllanthin and hypophyllanthin.
  
  
  CONCLUSIONS
  P. urinaria and P. maderaspatensis demonstrated dose-dependent hepatoprotective activity and hence, can provide promising therapeutic interventions against chemical-induced liver damage.
authors.affiliation: Department of Pharmaceutical Sciences, Gurujambeshwar University, Hissar, Haryana, India.
authors.forename: Surendra Kumar
authors.initials: SK
authors.lastname: Sharma
authors.forename: Sheela Meruga
authors.initials: SM
authors.lastname: Arogya
authors.forename: Deepak Hiraganahalli
authors.initials: DH
authors.lastname: Bhaskarmurthy
authors.forename: Amit
authors.initials: A
authors.lastname: Agarwal
authors.forename: Chandrasekaran Chinampudar
authors.initials: CC
authors.lastname: Velusami