title: Association of MSX1 799 G>T variant with nonsyndromic cleft lip/palate in South Indian adolescent patients.
abstract: BACKGROUND
  Nonsyndromic cleft lip/palate (NSCLP) is a common congenital anomaly with significant medical, psychological, social, and economic ramifications. It is an example of complex genetic trait. There is sufficient evidence to hypothesise that disease locus for this condition can be identified by candidate genes. The purpose of this study was to test whether MSX1 (799 G>T) gene variant was involved in the aetiology of NSCLP.
  
  
  METHODS
  Blood samples were collected with informed consent from 25 subjects having NSCLP and 25 controls. Genomic DNA was extracted from the blood samples, polymerase chain reaction was performed (PCR), and digestion products were evaluated.
  
  
  RESULTS
  The results showed a positive correlation between MSX1 (799 G>T) gene variant and NSCLP patients.
  
  
  CONCLUSIONS
  MSX1 (799 G>T) gene variants may be a good screening marker for NSCLP.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, BP Koirala Institute of Health Sciences, Dharan, Nepal. varundc@gmail.com
authors.forename: Varun P
authors.initials: VP
authors.lastname: Singh
authors.forename: Dinesh
authors.initials: D
authors.lastname: Ramu
mesh_terms: D000293:Adolescent; D020029:Base Pairing / Q000235:genetics; D002874:Chromosome Mapping; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D006147:Guanine; D006801:Humans; D051957:MSX1 Transcription Factor / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D017422:Sequence Analysis, DNA; D013941:Thymine
chemical_list: D005819:Genetic Markers; D051957:MSX1 Transcription Factor; C484083:MSX1 protein, human; D006147:Guanine; D013941:Thymine