title: Comparative transcriptomes profiling of photoperiod-sensitive male sterile rice Nongken 58S during the male sterility transition between short-day and long-day.
abstract: BACKGROUND
  Photoperiod-sensitive genic male sterile (PGMS) rice, Nongken 58S, was discovered in 1973. It has been widely used for the production of hybrid rice, and great achievements have been made in improving rice yields. However, the mechanism of the male sterility transition in PGMS rice remains to be determined.
  
  
  RESULTS
  To investigate the transcriptome during the male sterility transition in PGMS rice, the transcriptome of Nongken 58S under short-day (SD) and long-day (LD) at the glume primordium differentiation and pistil/stamen primordium forming stages was compared. Seventy-three and 128 differentially expressed genes (DEGs) were identified at the glume primordium differentiation and pistil/stamen primordium forming stages, respectively. Five and 22 genes were markedly up-regulated (≥ 5-fold), and two and five genes were considerably down-regulated (≥ 5-fold) under SD during the male sterility transition. Gene ontology annotation and pathway analysis revealed that four biological processes and the circadian rhythms and the flowering pathways coordinately regulated the male sterility transition. Further quantitative PCR analysis demonstrated that the circadian rhythms of OsPRR1, OsPRR37, OsGI, Hd1, OsLHY and OsDof in leaves were obviously different between Nongken 58S and Nongken 58 under LD conditions. Moreover, both OsPRR37 and Hd1 in the inflorescence displayed differences between Nongken 58S and Nongken 58 under both LD and SD conditions.
  
  
  CONCLUSIONS
  The results presented here indicate that the transcriptome in Nongken 58S was significantly suppressed under LD conditions. Among these DEGs, the circadian rhythm and the flowering pathway were involved in the male sterility transition. Furthermore, these pathways were coordinately involved in the male sterility transition in PGMS rice.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, North Road of Luoshi, Wuhan, Hubei Province, China. dyang@whu.edu.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Liu
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Guo
authors.forename: Gaoyuan
authors.initials: G
authors.lastname: Song
authors.forename: Qin
authors.initials: Q
authors.lastname: Cheng
authors.forename: Daiming
authors.initials: D
authors.lastname: Jiang
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu
authors.forename: Daichang
authors.initials: D
authors.lastname: Yang
mesh_terms: D002940:Circadian Rhythm / Q000235:genetics; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D017440:Photoperiod; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins