title: Identification of essential subunits in the plastid-encoded RNA polymerase complex reveals building blocks for proper plastid development.
abstract: The major RNA polymerase activity in mature chloroplasts is a multisubunit, Escherichia coli-like protein complex called PEP (for plastid-encoded RNA polymerase). Its subunit structure has been extensively investigated by biochemical means. Beside the "prokaryotic" subunits encoded by the plastome-located RNA polymerase genes, a number of additional nucleus-encoded subunits of eukaryotic origin have been identified in the PEP complex. These subunits appear to provide additional functions and regulation modes necessary to adapt transcription to the varying functional situations in chloroplasts. However, despite the enormous progress in genomic data and mass spectrometry techniques, it is still under debate which of these subunits belong to the core complex of PEP and which ones represent rather transient or peripheral components. Here, we present a catalog of true PEP subunits that is based on comparative analyses from biochemical purifications, protein mass spectrometry, and phenotypic analyses. We regard reproducibly identified protein subunits of the basic PEP complex as essential when the corresponding knockout mutants reveal an albino or pale-green phenotype. Our study provides a clearly defined subunit catalog of the basic PEP complex, generating the basis for a better understanding of chloroplast transcription regulation. In addition, the data support a model that links PEP complex assembly and chloroplast buildup during early seedling development in vascular plants.
authors.affiliation: Junior Research Group, Department of Plant Physiology, Institute of General Botany and Plant Physiology, Friedrich-Schiller-University Jena, D-07743 Jena, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Steiner
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Schröter
authors.forename: Jeannette
authors.initials: J
authors.lastname: Pfalz
authors.forename: Thomas
authors.initials: T
authors.lastname: Pfannschmidt
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D055786:Gene Knockout Techniques; D006720:Homozygote; D008954:Models, Biological; D008969:Molecular Sequence Data; D009149:Mustard Plant / Q000201:enzymology; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018087:Plastids / Q000201:enzymology*; D021122:Protein Subunits / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D021122:Protein Subunits; D012321:DNA-Directed RNA Polymerases