title: In-vitro acetylcholine release is not a straightforward model to study hippocampal 5-HT₄ receptors.
abstract: 5-HT₄ receptor (5-HT₄R) activation induces procognitive effects. This might be related to stimulation of hippocampal acetylcholine release, which has been shown for 5-HT₄R agonists in in-vivo models. We investigated the influence of the 5-HT₄R agonists, prucalopride and BIMU-8, on acetylcholine release in rat hippocampal brain slices. In contrast to the report by Siniscalchi et al., no facilitating effect of 5-HT₄R agonists on electrically evoked acetylcholine could be shown. The in our hands absence of an effect by 5-HT₄R agonists illustrates that an in-vitro evaluation of 5-HT₄R agonists on hippocampal acetylcholine release is not a straightforward model to study the relationship between hippocampal 5-HT₄Rs and hippocampal acetylcholine release.
authors.affiliation: Heymans Institute of Pharmacology, Ghent University, Ghent, Belgium. filip.devin@ugent.be
authors.forename: Filip
authors.initials: F
authors.lastname: de Vin
authors.forename: Joris H
authors.initials: JH
authors.lastname: De Maeyer
authors.forename: Romain A
authors.initials: RA
authors.lastname: Lefebvre
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D001572:Benzofurans / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D006168:Guinea Pigs; D006624:Hippocampus / Q000378:metabolism / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009924:Organ Culture Techniques; D017729:Presynaptic Terminals / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D044422:Receptors, Serotonin, 5-HT4 / Q000502:physiology*; D058828:Serotonin 5-HT4 Receptor Agonists / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
chemical_list: D001562:Benzimidazoles; D001572:Benzofurans; D019086:Bridged Bicyclo Compounds, Heterocyclic; D058828:Serotonin 5-HT4 Receptor Agonists; C406662:prucalopride; C070189:(endo-N-8-methyl-8-azabicyclo-(3.2.1)oct-3-yl)-2,3-dihydro-3-isopropyl-2-oxo-1H-benzimidazol-1-carboxamide; D044422:Receptors, Serotonin, 5-HT4; D000109:Acetylcholine