title: Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development.
abstract: Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein-trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full-length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm-derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development.
authors.affiliation: Division of Biology 139-74, California Institute of Technology, Pasadena, California 91125, USA.
authors.forename: Chathurani S
authors.initials: CS
authors.lastname: Jayasena
authors.forename: Le A
authors.initials: le A
authors.lastname: Trinh
authors.forename: Marianne
authors.initials: M
authors.lastname: Bronner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D058948:Cell Tracking / Q000379:methods; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D021621:Imaging, Three-Dimensional; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters / Q000235:genetics / Q000378:metabolism; D017385:Sequence Homology; D014741:Video Recording / Q000379:methods; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D027361:Organic Anion Transporters; D029961:Zebrafish Proteins; C570686:periodic tryptophan protein 2 gene homologue, zebrafish; C008822:citrin