title: Functional state of the respiratory and immune system in children-residents of the radioactive contaminated territories.
abstract: More then 25 years after the Chernobyl accident, a higher prevalence of bronchial hyperreactivity, reduced lung function, and increased levels of free radicals in exhaled breath condensates (EBC) were observed in children residing in radioactive contaminated territories. Comparing children with different residential radiation background, this study investigated fatty acids of EBC using gas liquid chromatography, counts of B-lymphocyte antigen CD19 in T-cell (CD3) and phagocytotic activity of neutrophils in blood samples. Regarding EBC, we demonstrate that lipid peroxidation was activated, antioxidant properties of pulmonary surfactant were decreased, were detected metabolic disorders of essential fatty acids at the stage of bioregulators-eicosanoids formation. Regarding the immune function of blood cells, we found a decrease of the proportions of CD3+ 19- and CD3- 19+ lymphocyte subpopulations and an unbalance of their numbers. Also the phagocytotic activity of neutrophils was reduced in higher exposed children. Children living in the radioactive contaminated territories have more alterations of surfactant properties and immune activities, which may contribute to an increased risk of respiratory problems. This research was supported by grants from the U.S. Civilian Research and Development Foundation (UKB1-2929-KV-08).
authors.affiliation: SI Research Center for Radiation Medicine of AMS of Ukraine, University of South Carolina.
authors.forename: I Ye
authors.initials: IY
authors.lastname: Kolpakov
authors.forename: V N Parkhomenko V Yu
authors.initials: VN
authors.lastname: Vdovenko
authors.forename: Ye I
authors.initials: YI
authors.lastname: Stepanova
authors.forename: D A
authors.initials: DA
authors.lastname: Bazyka
authors.forename: W J
authors.initials: WJ
authors.lastname: Karmaus
authors.forename: E R
authors.initials: ER
authors.lastname: Svendsen
mesh_terms: D000293:Adolescent; D018941:Antigens, CD19 / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D001826:Body Fluids / Q000737:chemistry / Q000166:cytology; D016535:Bronchial Hyperreactivity / Q000097:blood / Q000453:epidemiology / Q000276:immunology* / Q000503:physiopathology; D017252:CD3 Complex / Q000032:analysis / Q000276:immunology; D016022:Case-Control Studies; D047852:Chernobyl Nuclear Accident; D002648:Child; D045853:Exhalation; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000276:immunology*; D009688:Nuclear Reactors; D010587:Phagocytosis / Q000276:immunology; D015995:Prevalence; D012129:Respiratory Function Tests; D012137:Respiratory System / Q000276:immunology / Q000503:physiopathology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014455:Ukraine
chemical_list: D018941:Antigens, CD19; D017252:CD3 Complex; D005227:Fatty Acids; D005609:Free Radicals