title: Maspin and MCM2 immunoprofiling in salivary gland carcinomas.
abstract: BACKGROUND
  The pathogenesis of salivary gland carcinomas is very complex and prognostic markers are difficult to find in these carcinomas of which the different subtypes have varying malignant potential. The study was conducted to examine the cellular distribution of maspin and MCM2 in salivary gland carcinomas and their value to predict lymph node metastasis.
  
  
  METHODS
  Fifty three paraffin blocks of different lesions (15 muco-epidermoid carcinoma, 14 adenoid cystic carcinoma, 3 epi-myoepithelial carcinoma, 5 salivary duct carcinoma, 5 malignant pleomorphic adenoma, 6 polymorphous low grade adenocarcinoma and 5 acinic cell carcinoma) were prepared for immunohistochemical staining with maspin and MCM2 antibodies. ANOVA and Pearson correlation tests were used for the statistical analysis of the results.
  
  
  RESULTS
  All salivary gland carcinomas express maspin and MCM2 with variable cellular localization. There was a significant difference in the expression of each antibody between mucoepidermoid carcinoma, adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. No association was found between examined markers and lymph node metastasis.
  
  
  CONCLUSIONS
  Salivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization, consisting important markers of biological behavior in these tumors. The level of MCM2 expression can be used in the differential diagnosis of adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. Further study with large sample size is recommended to assess their value in prediction of lymph node metastasis.
authors.affiliation: Oral Pathology Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
authors.forename: Shaimaa E
authors.initials: SE
authors.lastname: Ghazy
authors.forename: Iman M
authors.initials: IM
authors.lastname: Helmy
authors.forename: Houry M
authors.initials: HM
authors.lastname: Baghdadi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D008949:Adenoma, Pleomorphic / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis / Q000473:pathology; D064111:Minichromosome Maintenance Complex Component 2; D009687:Nuclear Proteins / Q000032:analysis* / Q000096:biosynthesis; D012468:Salivary Gland Neoplasms / Q000378:metabolism*; D015843:Serpins / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; C085344:SERPIN-B5; D015843:Serpins; C084859:MCM2 protein, human; D064111:Minichromosome Maintenance Complex Component 2