title: Chromatographic separation and spectroscopic characterization of the E/Z isomers of acrivastine.
abstract: A reverse phase high performance liquid chromatography (HPLC) method has been developed for the separation of two geometric isomers of Acrivastine using crude reaction mixture. The resolution between two isomers was found more than 2.9. The geometric isomers have been isolated by preparative HPLC and characterized by spectroscopic techniques, such as NMR, infrared, and MS. The developed method has been validated for the determination of Z-isomer in Acrivastine. The limit of detection and limit of quantification of the Z-isomer were 0.05 and 0.2 μg/ml, respectively. The developed method is precise, linear, accurate, rugged and robust for its intended use.
authors.affiliation: Department of Biopharmaceutics, Zydus Research Centre, Ahmedabad, Gujarat, India. prakashdavadra@zyduscadila.com or prakashdavadra@yahoo.co.in.
authors.forename: Prakash M
authors.initials: PM
authors.lastname: Davadra
authors.forename: Batuk
authors.initials: B
authors.lastname: Dabhi
authors.forename: Manoj K
authors.initials: MK
authors.lastname: Singh
authors.forename: Mukul R
authors.initials: MR
authors.lastname: Jain
authors.forename: Hitendra S
authors.initials: HS
authors.lastname: Joshi
authors.forename: Atul H
authors.initials: AH
authors.lastname: Bapodra
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015203:Reproducibility of Results; D013057:Spectrum Analysis; D013237:Stereoisomerism; D014311:Triprolidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D014311:Triprolidine; C043648:acrivastine