title: Direct liquid chromatography method for the simultaneous quantification of hydroxytyrosol and tyrosol in red wines.
abstract: A validated HPLC method with fluorescence detection for the simultaneous quantification of hydroxytyrosol and tyrosol in red wines is described. Detection conditions for both compounds were optimized (excitation at 279 and 278 and emission at 631 and 598 nm for hydroxytyrosol and tyrosol, respectively). The validation of the analytical method was based on selectivity, linearity, robustness, detection and quantification limits, repeatability, and recovery. The detection and quantification limits in red wines were set at 0.023 and 0.076 mg L(-1) for hydroxytyrosol and at 0.007 and 0.024 mg L(-1) for tyrosol determination, respectively. Precision values, both within-day and between-day (n = 5), remained below 3% for both compounds. In addition, a fractional factorial experimental design was developed to analyze the influence of six different conditions on analysis. The final optimized HPLC-fluorescence method allowed the analysis of 30 nonpretreated Spanish red wines to evaluate their hydroxytyrosol and tyrosol contents.
authors.affiliation: IFAPA Rancho de la Merced, Carretera de Trebujena, Km. 2.2, Apdo. 589, Jerez de la Frontera, 11471 Spain. zulema.pineiro@juntadeandalucia.es
authors.forename: Zulema
authors.initials: Z
authors.lastname: Piñeiro
authors.forename: Emma
authors.initials: E
authors.lastname: Cantos-Villar
authors.forename: Miguel
authors.initials: M
authors.lastname: Palma
authors.forename: Belen
authors.initials: B
authors.lastname: Puertas
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis; D014920:Wine / Q000032:analysis*
chemical_list: C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; D010626:Phenylethyl Alcohol