title: A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.
abstract: A key obstacle to understanding neural circuits in the cerebral cortex is that of unraveling the diversity of GABAergic interneurons. This diversity poses general questions for neural circuit analysis: how are these interneuron cell types generated and assembled into stereotyped local circuits and how do they differentially contribute to circuit operations that underlie cortical functions ranging from perception to cognition? Using genetic engineering in mice, we have generated and characterized approximately 20 Cre and inducible CreER knockin driver lines that reliably target major classes and lineages of GABAergic neurons. More select populations are captured by intersection of Cre and Flp drivers. Genetic targeting allows reliable identification, monitoring, and manipulation of cortical GABAergic neurons, thereby enabling a systematic and comprehensive analysis from cell fate specification, migration, and connectivity, to their functions in network dynamics and behavior. As such, this approach will accelerate the study of GABAergic circuits throughout the mammalian brain.
authors.affiliation: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Taniguchi
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Priscilla
authors.initials: P
authors.lastname: Wu
authors.forename: Sangyong
authors.initials: S
authors.lastname: Kim
authors.forename: Raehum
authors.initials: R
authors.lastname: Paik
authors.forename: Ken
authors.initials: K
authors.lastname: Sugino
authors.forename: Duda
authors.initials: D
authors.lastname: Kvitsiani
authors.forename: Duda
authors.initials: D
authors.lastname: Kvitsani
authors.forename: Yu
authors.initials: Y
authors.lastname: Fu
authors.forename: Jiangteng
authors.initials: J
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Lin
authors.forename: Goichi
authors.initials: G
authors.lastname: Miyoshi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Shima
authors.forename: Gord
authors.initials: G
authors.lastname: Fishell
authors.forename: Sacha B
authors.initials: SB
authors.lastname: Nelson
authors.forename: Z Josh
authors.initials: ZJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology* / Q000378:metabolism*; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D017930:Genes, Reporter; D019426:Integrases / Q000378:metabolism*; D007395:Interneurons / Q000166:cytology / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid; C045073:Cre recombinase; D019426:Integrases